LOCUS       DADJYG010000001        68412 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.001, whole genome shotgun sequence.
ACCESSION   DADJYG010000001 DADJYG010000000
VERSION     DADJYG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.001"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            86..967
                     /locus_tag="JZ382_000001"
     CDS             86..967
                     /locus_tag="JZ382_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426216.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            980..2590
                     /locus_tag="JZ382_000002"
     CDS             980..2590
                     /locus_tag="JZ382_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAY2426217.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2667..3326)
                     /locus_tag="JZ382_000003"
     CDS             complement(2667..3326)
                     /locus_tag="JZ382_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAY2426218.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3609..4274)
                     /locus_tag="JZ382_000004"
     CDS             complement(3609..4274)
                     /locus_tag="JZ382_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426219.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4421..5026)
                     /gene="dhaL"
                     /locus_tag="JZ382_000005"
     CDS             complement(4421..5026)
                     /gene="dhaL"
                     /locus_tag="JZ382_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAY2426220.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5054..6037)
                     /gene="dhaK"
                     /locus_tag="JZ382_000006"
     CDS             complement(5054..6037)
                     /gene="dhaK"
                     /locus_tag="JZ382_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY2426221.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(6039..6434)
                     /gene="dhaM"
                     /locus_tag="JZ382_000007"
     CDS             complement(6039..6434)
                     /gene="dhaM"
                     /locus_tag="JZ382_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAY2426222.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6588..6908
                     /locus_tag="JZ382_000008"
     CDS             6588..6908
                     /locus_tag="JZ382_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY2426223.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7020..7889)
                     /locus_tag="JZ382_000009"
     CDS             complement(7020..7889)
                     /locus_tag="JZ382_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAY2426224.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7985..9289)
                     /locus_tag="JZ382_000010"
     CDS             complement(7985..9289)
                     /locus_tag="JZ382_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY2426225.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9286..9795)
                     /locus_tag="JZ382_000011"
     CDS             complement(9286..9795)
                     /locus_tag="JZ382_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426226.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9970..12105)
                     /locus_tag="JZ382_000012"
     CDS             complement(9970..12105)
                     /locus_tag="JZ382_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2426227.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12188..13843)
                     /locus_tag="JZ382_000013"
     CDS             complement(12188..13843)
                     /locus_tag="JZ382_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY2426228.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13845..14159)
                     /locus_tag="JZ382_000014"
     CDS             complement(13845..14159)
                     /locus_tag="JZ382_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY2426229.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14329..14589
                     /locus_tag="JZ382_000015"
     CDS             14329..14589
                     /locus_tag="JZ382_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAY2426230.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14603..14917
                     /locus_tag="JZ382_000016"
     CDS             14603..14917
                     /locus_tag="JZ382_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAY2426231.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15647..16201)
                     /locus_tag="JZ382_000017"
     CDS             complement(15647..16201)
                     /locus_tag="JZ382_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY2426232.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16327..18255)
                     /locus_tag="JZ382_000018"
     CDS             complement(16327..18255)
                     /locus_tag="JZ382_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAY2426233.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18561..19277)
                     /locus_tag="JZ382_000019"
     CDS             complement(18561..19277)
                     /locus_tag="JZ382_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAY2426234.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19278..21101)
                     /gene="glpO"
                     /locus_tag="JZ382_000020"
     CDS             complement(19278..21101)
                     /gene="glpO"
                     /locus_tag="JZ382_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAY2426235.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21115..22611)
                     /gene="glpK"
                     /locus_tag="JZ382_000021"
     CDS             complement(21115..22611)
                     /gene="glpK"
                     /locus_tag="JZ382_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY2426236.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22747..24207)
                     /locus_tag="JZ382_000022"
     CDS             complement(22747..24207)
                     /locus_tag="JZ382_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2426237.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24197..25492)
                     /locus_tag="JZ382_000023"
     CDS             complement(24197..25492)
                     /locus_tag="JZ382_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY2426238.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25824..26501)
                     /locus_tag="JZ382_000024"
     CDS             complement(25824..26501)
                     /locus_tag="JZ382_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAY2426239.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26531..27622)
                     /locus_tag="JZ382_000025"
     CDS             complement(26531..27622)
                     /locus_tag="JZ382_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAY2426240.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27723..28157)
                     /locus_tag="JZ382_000026"
     CDS             complement(27723..28157)
                     /locus_tag="JZ382_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2426241.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28741..30198)
                     /gene="ascB"
                     /locus_tag="JZ382_000027"
     CDS             complement(28741..30198)
                     /gene="ascB"
                     /locus_tag="JZ382_000027"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY2426242.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30195..32090)
                     /locus_tag="JZ382_000028"
     CDS             complement(30195..32090)
                     /locus_tag="JZ382_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2426243.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32148..33923)
                     /locus_tag="JZ382_000029"
     CDS             complement(32148..33923)
                     /locus_tag="JZ382_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY2426244.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33973..35469)
                     /locus_tag="JZ382_000030"
     CDS             complement(33973..35469)
                     /locus_tag="JZ382_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2426245.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35663..37285)
                     /locus_tag="JZ382_000031"
     CDS             complement(35663..37285)
                     /locus_tag="JZ382_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY2426246.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37734..39047)
                     /locus_tag="JZ382_000032"
     CDS             complement(37734..39047)
                     /locus_tag="JZ382_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY2426247.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39292..39516)
                     /locus_tag="JZ382_000033"
     CDS             complement(39292..39516)
                     /locus_tag="JZ382_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426248.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39536..39904)
                     /locus_tag="JZ382_000034"
     CDS             complement(39536..39904)
                     /locus_tag="JZ382_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAY2426249.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(40101..40811)
                     /locus_tag="JZ382_000035"
     CDS             complement(40101..40811)
                     /locus_tag="JZ382_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426250.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40829..41326)
                     /locus_tag="JZ382_000036"
     CDS             complement(40829..41326)
                     /locus_tag="JZ382_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426251.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41363..41815)
                     /locus_tag="JZ382_000037"
     CDS             complement(41363..41815)
                     /locus_tag="JZ382_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2426252.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41950..42555)
                     /locus_tag="JZ382_000038"
     CDS             complement(41950..42555)
                     /locus_tag="JZ382_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAY2426253.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(43005..43625)
                     /locus_tag="JZ382_000039"
     CDS             complement(43005..43625)
                     /locus_tag="JZ382_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2426254.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43627..44496)
                     /locus_tag="JZ382_000040"
     CDS             complement(43627..44496)
                     /locus_tag="JZ382_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAY2426255.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44519..44926)
                     /locus_tag="JZ382_000041"
     CDS             complement(44519..44926)
                     /locus_tag="JZ382_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY2426256.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44945..46234)
                     /locus_tag="JZ382_000042"
     CDS             complement(44945..46234)
                     /locus_tag="JZ382_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426257.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46261..46563)
                     /locus_tag="JZ382_000043"
     CDS             complement(46261..46563)
                     /locus_tag="JZ382_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2426258.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46588..48681)
                     /locus_tag="JZ382_000044"
     CDS             complement(46588..48681)
                     /locus_tag="JZ382_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAY2426259.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49170..49934)
                     /locus_tag="JZ382_000045"
     CDS             complement(49170..49934)
                     /locus_tag="JZ382_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAY2426260.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50045..50914)
                     /gene="mdcB"
                     /locus_tag="JZ382_000046"
     CDS             complement(50045..50914)
                     /gene="mdcB"
                     /locus_tag="JZ382_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAY2426261.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50939..52015)
                     /locus_tag="JZ382_000047"
     CDS             complement(50939..52015)
                     /locus_tag="JZ382_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY2426262.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(52039..52632)
                     /locus_tag="JZ382_000048"
     CDS             complement(52039..52632)
                     /locus_tag="JZ382_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAY2426263.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52636..54312)
                     /gene="mdcD"
                     /locus_tag="JZ382_000049"
     CDS             complement(52636..54312)
                     /gene="mdcD"
                     /locus_tag="JZ382_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAY2426264.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54302..54601)
                     /locus_tag="JZ382_000050"
     CDS             complement(54302..54601)
                     /locus_tag="JZ382_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAY2426265.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54703..56349)
                     /gene="mdcA"
                     /locus_tag="JZ382_000051"
     CDS             complement(54703..56349)
                     /gene="mdcA"
                     /locus_tag="JZ382_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAY2426266.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56356..57267)
                     /locus_tag="JZ382_000052"
     CDS             complement(56356..57267)
                     /locus_tag="JZ382_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY2426267.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57387..58244
                     /locus_tag="JZ382_000053"
     CDS             57387..58244
                     /locus_tag="JZ382_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2426268.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58431..58793)
                     /locus_tag="JZ382_000054"
     CDS             complement(58431..58793)
                     /locus_tag="JZ382_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426269.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(58982..59164)
                     /locus_tag="JZ382_000055"
     CDS             complement(58982..59164)
                     /locus_tag="JZ382_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426270.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            59139..60578
                     /locus_tag="JZ382_000056"
     CDS             59139..60578
                     /locus_tag="JZ382_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY2426271.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60585..63137)
                     /locus_tag="JZ382_000057"
     CDS             complement(60585..63137)
                     /locus_tag="JZ382_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAY2426272.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63251..64582)
                     /locus_tag="JZ382_000058"
     CDS             complement(63251..64582)
                     /locus_tag="JZ382_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2426273.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64548..66026)
                     /locus_tag="JZ382_000059"
     CDS             complement(64548..66026)
                     /locus_tag="JZ382_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2426274.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66331..68166)
                     /gene="tdc"
                     /locus_tag="JZ382_000060"
     CDS             complement(66331..68166)
                     /gene="tdc"
                     /locus_tag="JZ382_000060"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAY2426275.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19466 a        14586 c        11706 g        22654 t
ORIGIN      
        1 agttttataa aaaggtataa aaccttttta ttaaactatt taatcttgtt tagtatatat
       61 ttttagaaaa aggagtgggg tttttatggc agaaatcgtc aaagtcacac atttgaaaaa
      121 gagctttggt cgtttcgaag ctttaaaaga tgtctctttc accgtacatt caggtgaagt
      181 cgttggattt atcggaccaa atggttctgg aaaatccacg acgattcgga cacttttagg
      241 gattataaaa aaagacggcg gaacggcaac gatttttggg caagatgtat gggaagatag
      301 tttatctatc catgaacgtc tttcttatgt tccaggcgat atctctttgt ggggaaattt
      361 gactggagga gcgatcatcg atctcttcat gaagcttcat ggaaaaggga gtgctcaaaa
      421 aaaagatgaa ctgattcaac gtttctctct tgatcctaag aaaaaagcaa aaaactattc
      481 aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg gctgttgatt cggatctcta
      541 tttatttgat gagcctactt ctggtttaga tcctttgatg gaaacgattt tccaagaaga
      601 agttgaaaaa ttgaaagaac agggcaaatc catcctactt tcttctcata ttctcagtga
      661 agtcgagcga ctagcggata aagtcgtgat catacgcgaa ggaacactcg atgaagaagg
      721 aacactcgat gaattgcgtc atttgactcg ttcgaccatc actatagaaa cagaagcaga
      781 gaatagtcac ttagcacaat tgaacggtgt ccatgacttt gtgcaaaaag gtcacctcgc
      841 tcaatttgct gctgataacg aagctttcga tgtgatttta gctgaagcgt ccaaattagg
      901 tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat ttattcatga gacattatga
      961 aggatgagga ggaagcgaaa tgactgataa attttcacgc tggtccaccc ttttttctca
     1021 aaaccttagc cgtgactgga aaaagatcct gatatggcta gtcggtgtca ctgctttttc
     1081 tggtggtttt gtccctgcgt ttgaagaatt gacttccgga caaggattag ctggattata
     1141 cgaaaccttg caaaacccgg caatgattgc aatggtcgga ccaactccag cttcgaatgc
     1201 tgctgagtat actttaggag cgatgtatgc aaatgaaatg ttgctttttt gcagtttgtt
     1261 tgctgctgtg atctctgttt tacacgtggt gagccaaacg agaaaaaaag aagaacttgg
     1321 tctgattgaa cttattcgtt cctttcgagt cggacgtcaa gcaaactctt tagctgtcat
     1381 cgctgaaagt attctgatca atttgctttt agcaatatcg atcagctgta cgatgatttt
     1441 tttccaagcg gattcgatca ctgcagaagg tgcttggctt tttgggctat ctgtaggtag
     1501 tatggggatt ttggggagtg ccgttgcact gtttgctgca caactgatgc caagttcagc
     1561 cagttcgaat ggtctgtctt tagcgttttt aggtggactt taccttctac gagcaataac
     1621 agatgtagct gattcctccc tttcgatgtg gaatccttta ggctggtctt atttgaccta
     1681 tccatttaca actaacaact ggattccttt gctttatttg tttttgttca gtctaatctt
     1741 gataatcagt tcatttgttt tagaggaaca tcgagatttg aacaccggct atatccccga
     1801 acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc ttgcgtggtt tattattcta
     1861 tctaaataaa gggatgacaa tcagttggct tatcactttt ggtttgatgg gggcggcata
     1921 cggctctatc tacgggaata tgcaacagtt tttagaaagt aacgacttaa tgaaacagat
     1981 gtttatccaa tctggcgtct caattgaaaa aaactttaca agcacgatca tggtagtctt
     2041 gatttgtcta gctgctattt taccaattgc tagcatcaat cgtttattta cagaagaaac
     2101 ccacggacgt atgaatcagc tgttcgcaac aaaaatttct aggaaccaac tttattggac
     2161 caatatgtta ctagctattc tatttgctat attggcaatc ctgattagtt ctggtgcact
     2221 agggctttct gctttggctt cgatgaagga tgcgaaagac atggatctat tgatgtttct
     2281 taaagccggg ttcaatttcc tgcctgccgt tttagtgttt atcggaatta gtggctttct
     2341 attcggatgg cttcctcgaa tcggtaaact tgcttatatc tacttagctt actcctttgc
     2401 actcaattac tttggcggaa ttttggattt accagaatgg ttttccaaaa cagctgtccc
     2461 tagctgggtt ccacggatgc caaacgattc ttttgatctt tccgtgttcc ttacgttatc
     2521 atgtatcagc cttgcactgt tcgtccttgg ttctattggt tataggcgaa gagacttact
     2581 agaaggataa taaaaaagca tgtacaattt catgctagcc tccaaatctg ggtcaaaaat
     2641 ccaacatttg gaggtcattt ttttatttac aagtcttcta actgctcttt atcaatgatc
     2701 cagacttctc gattctttct ttttacataa ccattctcct ctaataattt aaattttcta
     2761 gataaagttt cgggggtcgt accgataaat gcagcaagtt ctttcatttt cattggcagg
     2821 aaaaagtgat ccttccctgt cgctttagct aagtcaagca gatagacagc gactcgttct
     2881 tctacttttt ccatcatcaa aaaagttgtc tgctgctgta cttttgttaa tttctcaacg
     2941 ttgattgcta aaagtttcag actaagggcg ggataatgca gtaagagttc cgagaaatct
     3001 ttttgtctca atatgcaaat ttccgttttt tctagtgctt cgccaaataa agactcattt
     3061 ggaacgccta gcaactggct ctctccctca tatccgcccg gctctattac tcgtaaaagc
     3121 tgttcctttc cgctagcaga taattgatac actttcatac tgccggaggc cacgatcact
     3181 aattgaggtt ctccattcgg cgcaagaatc tgttctcctt tttccacata gtgatgtttg
     3241 acaagtgcgt gtactttttc ttgatcttgc tgtactaagt gattgaacaa aggaacaaat
     3301 gaaacacata aatgatcatg actcataatc tctcctcctt ctaaacagct atttctaata
     3361 aagaaacata acttccctta ttccaactga taattttctt ttatcatagc ataaacgtca
     3421 agcgaaccag ataccagcaa atagagtgga tttctttttg tcccttgctt tattttcctt
     3481 cttttgagac cttaagtaat tgattcctat caatttttta aatatttact ttttgaataa
     3541 tcaaaaacaa accttcactc gcaagccgag agtaaggttt gtttttcgca ttgcttattt
     3601 cgtgttgtct attgaaattt ttccgcacct agcttttgat atagtggtgc tagttcttgt
     3661 aaggtttcag cggttatttg ctcaacgtgt tcgatttctt gcggatagaa gaatagttta
     3721 cccatttgaa aggttcggtc tctcatcgct tttttcaact cttcttgttt ctttgtatcg
     3781 gacaaatagt cagacaaagc taagtggtcg tcaagttcat gttctggttg cggccatatg
     3841 taataatcct tgatatcgac tgttttgctc cactctttca gtatcgcaag caactggtta
     3901 tacgctgcaa tgctaccata tcgttcttcg cttttataat gttggtacat caaatagatt
     3961 tggtattgtt tttgattcaa caaaactgct agacaggcat tctcgtcttg acgagattcg
     4021 cttcggtacg cgctccaaaa atgactgcgt aaattccagc cattcgtcca gctttcgatt
     4081 ttaggtttgc tcatgccgta catatcaggc aaaagtgcag ctgtctggag ctgaatctct
     4141 ttccattttt gccagtgttc tttatattct ttcttgattt gttcaatctt atctgttgga
     4201 tggctctctt tgagctgttt gaatgcgtag attggtcgat caaacaactg ataatctgat
     4261 tttccaagtt ccattgtttc gcctctttcc gttatctttt gtattctacc tgaattttgc
     4321 gatatggaca aatagaggtt gtgaaaaagc tgttctgcat caagtcataa gaacagccaa
     4381 acaaaaaact gcgatgagca tcaccgcagt tttattgaac ttaaggttga ttgatcacat
     4441 ctaaaagcgt tgaaaataaa atggcactag aagcagctcc tgggtcgata tgtccgattg
     4501 cacgctctcc tagataggaa gctcgtcctt ttttagctac caagactttg gtgtgttctt
     4561 ttgcctggtc aatgatttct tttgttaaat tccctgcctt caatgcctct gtgactggca
     4621 gccaaacatc taacatcgtt ttatcgtcag cttctgcctt tcctcggctt tgaatacccg
     4681 ctgttccctg ctcgatcact tcgcctaatt tttcttgaga atcaatagaa tcgacctctt
     4741 ttgtcgcttt catcatgttc atgaatgcag atccgtataa cggaccagaa gccccaccga
     4801 ctttcgatat catcgccata gagaatactt tgaatgtatc gctgattgtt tcaggatgat
     4861 cggcttgaaa agcttcttca taagctgaaa cccctcttcc catgttattt ccatggtctc
     4921 catcgccaat cggggtatct agatcactga gatattgttt gttctgattg attgcctctg
     4981 caaattgcgt gagccattct tggatttctt gaacggttag tttcatatta tcaccctttt
     5041 atcttgagat tttttaccat gaaatcgtct gtacattgct ttctaaagaa tctttccatt
     5101 gtgtctccgt caagtcgatc agtgttagag aaagaccttg catatctagt gaggtcataa
     5161 aattaccgac ttttcggaaa actacattga cgcctctttc ttccaacaga gctaaaacat
     5221 cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc cattccgttg actagtacac
     5281 ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt agtaacaagt tcctttgcca
     5341 gtactttaga aggctgcatc ttttctcgac gatagcctgg ctcaccgtgg ataccaacac
     5401 catactcgat ctcatcttct cctagttcga atcctggttt ccctacttct ggtacagttg
     5461 ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac gaccttttct cccagttctt
     5521 tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc cacgattttg tgtacaagga
     5581 cagtacctgc aactccgcgt tttccagcag tatacgtact attttcaacg gcgatatcat
     5641 catctacgat cacactttct acttcaatat cgtccatagc agctaattcc tttgccatat
     5701 cgaagttcaa tgcatcccct gtataattct taacgatcag caaaactcct tttccactat
     5761 cggcttcctt aatagccgtc tgaatctgat ctggagttgg agaagtaaac acatcaccaa
     5821 gcaccgctgc acttaacatc ccttccccta caaacccggc atgggatggt tcatgtccgc
     5881 ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg attcttggct acaacacggg
     5941 aagattctac acgatgaaca agttctggat agctttttac taatccttcc agcatttctt
     6001 cgacaatatt gcttggatca ttcatgattt ttttcaattt attcccatcc tttctttccg
     6061 gcattttgtg cttctgttaa gatttgctga gtatcatctg ttataccggc agtgatggct
     6121 gctgcaaaag ctccttccac taaaggcgca tccaccagat gataacgttg ctgctgctct
     6181 tcttccaaca tgtcttttgc cagttccgag ttcaataccg cgctaccgat atccgcaaaa
     6241 acaaaataac tagctgcatc gcttgattca ttgactgctt ctacgatttt catcgggtct
     6301 gatccgatct cgccatttat tccgccgaga gaaaaaattt gcacctcttc ggatttttgc
     6361 atctgctcaa tcatttcttt aataccatct gttacttttt gactatgaga aataagcaaa
     6421 atactttttt tcacaagaat cttcctttct tttacatagt tgctaacgat tggtttcgct
     6481 ttcattaatg aacaattcaa gtatataata aatttctaat tttgggaaaa tatgtgtagt
     6541 gcttcttgaa gtttgttata ctttaattaa aagagaggag tgatcgaatg attacaacaa
     6601 caacgaacag tgtagaggga aaagccgtag tagcttataa agggatcgtt tttggtgagg
     6661 tcattactgg gatccatgca tttaaagact tggaggcggg gttgcgaaat atctttggcg
     6721 gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag agaagaagca cttgccgaaa
     6781 tggccgaacg tgcaaaagca ttaggagctg atgcgatcat tggcgtaaaa atggactatg
     6841 aagtattagg cagcgacaat ggcatgctga tggtcacatg tagtggaaca gctgtctcca
     6901 cgagataagt aggggacatc tgttttaata actaaagaga gatgggacat gtagtggcac
     6961 tcaagaatta gatttttcag tctaacttcg aggtactcta ccttcccatc ctctcttttt
     7021 tatttctttt tgcggatgat tccgtaagct gctgcgacta acacacccgc ccctgctaag
     7081 gaataactga cagaagctgt tttttcattt gtttttggca aagtctgact tgttgatttg
     7141 gatggctctg tcgtttgctg aactgtttct gtcgtgcttc cagaggtcac tgatttttct
     7201 cctgccgaat gcaaaatatc ttttattcgt tggaaactag ctgctaagtc agcttttttt
     7261 gcttcaaaat cattagcaac tgtttgtgct tcgctgatct ttccgtggat tgcttgactg
     7321 gccgctgtga tatccgcttg agtttgactt agctttccag aagctgtttg agcaacctga
     7381 tttaattgct gattgaattc ttttgcttgt tggatcttcc cgtcaagatc tgtttggaca
     7441 gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa gttgtgcacg gtcatttccc
     7501 actttatttt taagatcagc taaatcttga tcgattgctg cttggtcttc tttcaatttg
     7561 ttttttgcag cttctaatgc ttcttcgagt tcttttcttt tattttccaa gtcttgcaaa
     7621 tgatcgattt ggtcagaaga ggtagaacta tttattgatg tatcttctga agatgaaatc
     7681 gtttctcctg aattatctgt cggtacattg gaagtcattt ctgtttccga ggcaccgttt
     7741 tcagcaccag aagagcttat ggttgttgaa ggttctgttt gagcaggttc agaagtttcc
     7801 gcaaaagctg tctgtcccaa taagaaaata ctggaaacag caattggcca gattaacggc
     7861 ttcattcttt ttttcatcac ttttcccatc cttactactt tttaactaat ttcttattat
     7921 aagcaaaaat gtttggaaga accataaaca caacaggctg tttgtctaaa aaacggtgca
     7981 tcttttattt ttttgtacgt tccgggcttt tccatgtccc gcttccataa cctaaaatcg
     8041 ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc gttcactatc cagaacaaaa
     8101 gcatatatag tggtgcgaaa agaaggtatt tcaattttct tcgtcgatca tctacaatca
     8161 gcgaagctag caactggaaa aatcctgcga ccatttcaaa gcagatgaac acaaaactga
     8221 atgtaaaagt aatataaatc tgttcgtagt ttccttgata tacataatgc ccgattaatc
     8281 cagcaaaaag aactacggaa atacaaaaga atattgacca gacaatactc aatgtttgat
     8341 caatgaagat gatcgttctt ccaatatgtt taaaaggatg gagcatcact tttttgaaat
     8401 tcgtcagcca gacttctgtt ccaccttttg cccaacgttt tctgtgtcga tatagcatct
     8461 tcaacgtcac aggtacttcc atgaaaaaga taatctcagg agcaaaaaca gataaccagt
     8521 catttaactg atgatcccaa gcaatactga tgtcttctgt cgctcgatct tgtctgaatc
     8581 cgcccacatc gatcaaggct tcttttcgat acatcgtatt tgctccactg tacgcataaa
     8641 gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc cacgatactt gagaattcaa
     8701 ccgtttgaga ttttgcaatc aatttggtac gattttggac atccatattt gcagtgaccg
     8761 cagcaatatg tcttgcacca gggcgaatga aataattcac atacctaatc agtgcatctg
     8821 gctccggtac cgtgtctgca tcattgctta aaatcaattt cccttttgca aaggcaagtc
     8881 cgatattaaa cgcgtgggct tttcctttat ttttttcgat ccgaaccaca cgaagattag
     8941 cgtatttttt catcagtcta gctaatattt ctggtgtttg gtccgttgaa ccatcgtctg
     9001 tcactaatac ctcatagttg tgataattca atttcgtcat caaatactcg attgtatatt
     9061 cgatcacaat ttcttcatta tgggctggca ccatgatcgt gatgaacggt tcgacactca
     9121 agggaacatc tacccattct ttttgcttat gtttgtataa aaacacataa caccagacac
     9181 caataaacca tacaaatccg cctaaaatcg gataagaaac gagaaaaaaa tacataatag
     9241 acaaacaagt atctaagaat aaattacctg tatgggtgat tgagatcatt tcagttccac
     9301 cccattttct ttatacaaat ctgcaatgaa tgtcgtatct agatttttct cttcggagac
     9361 agaataaaaa cggaccgttt ctctctcttc ttttgttcca aaacgttccg tatacacttc
     9421 ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca tattcaaaag atttatttac
     9481 ttttttgtat agatttcgat tgttccacct agtcaataca aatgaggtaa tcaggaaact
     9541 gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc attttctttt ctgataaaaa
     9601 agcgtacaag gaaaaatcct tggctctttc tggtctcgtt aatgggaaaa agacccatat
     9661 aaaaggaaga acaacgatca tccatcctaa aagagccact aacgtttgcc aaatctttaa
     9721 ccagtaatta ccttttgaaa aatacttatc tttcaccaaa attgatgcaa cttcttctct
     9781 tgttactttt ttcatatcct cacctcacgt ttttttgtta cgacgtagga gtttaccata
     9841 catttcattt cttttcacat aaagtgttcc taaatttttc ctaagaaaaa gaacaaaaca
     9901 tttctcatca gcaaatgaaa aatattttgt tctttttatc agttgttctt tttatttttt
     9961 atctcttaat tatgttgctc tttccaatac gacaatgctt ttcattcctc tagatatcgc
    10021 cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg ttttcaaatg gatataagat
    10081 tacatgatca aattccagtc cttttgctag actcaaagga atgatttggc agttttctaa
    10141 tccatatgaa ttttttttaa tcgattcttg tagcgtttgg gtttcctctt ctgtttgggt
    10201 gataactgcc gctttttctc cttttagaga aaccaaaatc tcagctagta ttttcaacaa
    10261 ttcttcagag gatcgaaaag agcggtattc gggttctttt ccttgcggtc ggatcgatac
    10321 gatgtcgatc ttctcttgat ccacagcata ggttttgaaa agctctgtga ttgctccgct
    10381 agaacggtaa ctatttctaa gatcataccg atggatcggc agatggtagt cgtcgaaaca
    10441 acgcataata tcagcaaaag tagtggaaga attaaagatt gcctgattct cgtcacctac
    10501 taaagtaaag tgagtttttg gaaataattc gatcaataga cctaattgtg ccagggtata
    10561 atcttgtact tcatcaatca atagatagcg aaatgctggt acatttacct tttcaacaag
    10621 taaatgacga atagctaata taatgactgc ttcatcaaca gtacgtgtgg gttgccaagc
    10681 ataagctgag ccatatcgtt tttcataaat tctttcaaac aactgatgct catccaccca
    10741 tgccatttct tcgatttgtc tggttatttt tcgatatttt ttttgtaaaa gtttccttgc
    10801 ataagctaca atactttgtt ccgaatcatc agaaatcaat ttaccgaata attccaactg
    10861 cctatcttct gtcaacgaga gtacctgatt ctgcacagca ggactttttg cttgtttcaa
    10921 aagatgattc ttccattcgc tcatcagtgc ctttttcgtt gcttgaagcc tatcgatcaa
    10981 tttaggatga gcaggaactt tctcatatat tttttcgatc gtttttttag aaaggactgt
    11041 ttttccattt ttagtaagtc cacggataaa attcggatga tctaaaaaca aaggatctga
    11101 ttgttttaga ttatcgataa actttttgct acgcaaacgt tctgtctgct ccgatacatt
    11161 ttctcccgtg atacgtttga aataagcctc ttcaccttct atttcctctg ctgaaagctg
    11221 tgaaaccagc tgtatgatgg ttagattcag tggatttctt tcacctaatg atggtaggac
    11281 attcgaaata tagtcgataa accgatgatt aggtgataga atcagcaagt catcggatgt
    11341 catcgtatct cgatataaat agagcagata cgcaatacgc tgcatgatag cagaggtttt
    11401 accacttcct gctacgccat ttaccaagat attttgactt ttcacatccc gaatgattgt
    11461 gttttgttcc gattggatcg tagcagtgat agccttcatt tcattggtct catcttggcc
    11521 taagacttct aataatacgt catcttgaat agctacagac gtatcaaaaa acttgaccaa
    11581 acgatctctt tccaaaagaa attgtcgtct ttctttaata accgtatcga ttttattttg
    11641 acgaaccgta taagaagatg gaccaatcat gttattgtaa aaaagttcgg caattggtga
    11701 acgccaatca taaacaagat tttcatctgc atcatctgtg aaatttgctg taccgatata
    11761 aaaggattct tttggctcat tatccagaaa gtctacaacg acttttccaa agtagggact
    11821 tttcaataaa cgttcgatct ttgctagctg attttctgcg tttttctgtt tgatattcat
    11881 ctgatcgatt tctctatttt tcatttcaat catcgaaaaa gtatccaggt tatctaagta
    11941 actgtcaaaa ttcaatcgaa gctcgccagc catttctttc aacgactggt ttccttctat
    12001 ttttgccgta tccatcgatt cccgcagttc gtttttttta tcaattagct tttgatacac
    12061 gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg accatacaca ttctcctctt
    12121 tttttaaagt ggtggaaccg attagtttac ctcttttaag agtcgaatgc aagcttttta
    12181 acgagtatca tttgttagga tatatcagtt cttcaggtcg gacagtttga tataaagaaa
    12241 atgcaccatc aaggtttttt acgtggtagc cagactgctt caagattctt tccgctagat
    12301 agctacgtaa tccgctatga cagctgacga tatattcttg ttggctgtct aattcattta
    12361 atcggtcccg aagttcattc aaagggatat gtttggcttc cttgaatttg cccttttcca
    12421 attcttctgc tgttcgcaca tctaataaaa tttttccttt tgctagttct gtcggcaatt
    12481 catgccattg tacggtttca ctaagccctt ctgcaatgtt cattgctgca tatcctagca
    12541 tgtttactgg atctttggct gagccaaatg gcggtgcata cgtcaattct aattccggta
    12601 aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa aatatcgatc cgtttatcca
    12661 caccttttgc cccaacacct tgtgcaccat aaatctcgcc agtctctgga tggaagatca
    12721 acttcagtaa aatatctgtt gcttctggat aatagcttgc atgatctttt cctgatacat
    12781 gaatgacttc aaaagacagt tcgttttctt tggctgttct ttcacttaaa ccggtggatg
    12841 cggcagtcat tccaaacgtt cgtataattg ctgttccaat accgccttga tttcttcgag
    12901 aaattccgga aatcgtgtcc gcaacttgtc taccttgacg attggctggt gaagcaagag
    12961 aaataagtgc atcttttcca gtgatttgct gttttacaac gatagcatcc cccaccgcat
    13021 aaatatcagg aatattcgtt tgataacgtt cgtctactag gatacccccg cgtatgccta
    13081 attcgattcc agcggtcact gctagctggt tatcaggacg aacaccaatg gataaaataa
    13141 ctaaatcagc agggatggta cgcccatcat ccagtcgcaa tcgtttcccg tggtcttcaa
    13201 atccagcgac agcatgagaa gtaatcactt ctactccttg atgagacaat tctttttcta
    13261 taaaagctgc catttcttca tccagtgttg gaagaatatg tggtgcttgt tcgatcacca
    13321 tgacttctaa acctcttctt tttaggtttt ctgccatttc caatccgatg aatcctgcac
    13381 caacgatcac ggcacgcttt ggctgttttt ctaatgcatg catcacttta tcgatatctg
    13441 gtacatttct aatagaaaaa acattgtcgg cttctgttat ccctggaatc gatggaacaa
    13501 ctggttttgc accaggagat aaaaccaatg catcgtactg ttcaatgtgt ttttgaccct
    13561 catgtacgac ttctaccgtt ttgttttcgg gaaagatggc tgtcacttca tgatttggac
    13621 gcacatctaa acaaaaacga gcggataatg attctggcgt ttggacaaga agattttccc
    13681 gttcactgat ttctcccgaa agataataag gcaagccgca atttgcaaaa gaaacatacg
    13741 gtcctttttc aaaaacaacg atttctgcat cttccattaa tcggcgaagc cgtgtagcag
    13801 cagacattcc acctgctaca cctccgacaa taacgatttt catcttattt ccctcctact
    13861 gttcgatcaa accattgatt catgccacca cgtacattca ctacatcata accagatttc
    13921 tttaattctt tagctgcttg tttactcctc atccctgatt gacaaatcac atagacggtc
    13981 ttttctttgt ctccatcata tctgtttata ctttgtaatg gaacattttt agctcctttt
    14041 atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa gtaacgctgg attttccaaa
    14101 agcttttcct ttaattctgg gactgttatc gattttattg attgaaatag caaattcatt
    14161 ttctgcacct catttacatt tttataaata cctcatgggg tatattaaac aggagcttta
    14221 ttaatatgtc aaccagaatg actcttcatt tttttcacta ctttttatat ttgcataaaa
    14281 taccgataag gggtattatc taattataaa aatagaggag aatgtgttat ggagattgat
    14341 aataaaaata tcctgaaccg tttgaaacgt acagagggac aaattcgtgg tatccaaaaa
    14401 atgatcgaag acgaaaaaga atgtatggat gtcatcactc aattaagcgc tgttcgttct
    14461 agtattgatc gcgtaatggg catgatcgtt gcagataatc tgaaaaattg ctttgagaaa
    14521 cctgatagta atccagaaga acaagcggct aaactagaac aagcgatcaa gatgattatt
    14581 aaaaagtaaa ggaatgattt caatgaaagt cgttttccat attgatgaat tagaaaaatg
    14641 gtcagaaaca gggaaaaacg taaaaaacct gataaaagct tctccagaaa cgacgattgt
    14701 cgtatctgta aatggcatcg cgattaccgg ttatctagac tctgccaatg cagaattctt
    14761 agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct atgcgtgcta atcatatttc
    14821 agaaagttct ttacctgaac aagtaatcgt tgtaccagca ggtgtccttg atttagttga
    14881 actgcaatcc caaggttatg cttatatcaa gccttagtca gtagaggttg cgacagaagt
    14941 gatcagcttc aagaaatttc ggcttatttt tcgaagaagc tacttctgga aaaccgtgaa
    15001 tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag aacagccaaa caaaaatagc
    15061 ttctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    15121 taaggaagag gttgtgaaat cagcggtttg acctaagtat tagagaaaaa aacgggaaaa
    15181 tagctcctca tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg
    15241 tttaaggaag agattgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
    15301 aatggatttc tacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaata
    15361 ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg agtattagag aaacaaacgg
    15421 aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    15481 caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc
    15541 aaaaacaact tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga
    15601 gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa
    15661 acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga
    15721 cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca
    15781 tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga
    15841 tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata
    15901 gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt
    15961 gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc
    16021 tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg
    16081 ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga
    16141 atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca
    16201 ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa
    16261 aaccaatgat ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg
    16321 agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc
    16381 ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg
    16441 tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc
    16501 tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac
    16561 atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac
    16621 aaatatccga tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt
    16681 aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta
    16741 acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc
    16801 ttggctttct cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc
    16861 gaaagccata gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc
    16921 ataatgggtc gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat
    16981 cgctagtgca gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa
    17041 cgtattcctt tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt
    17101 actcaacgca ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac
    17161 agcaagctct gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa
    17221 taaaccgact gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa
    17281 gcctcgatag tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg
    17341 atgtctgcct tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt
    17401 gatttctcgg ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat
    17461 caataaatcg atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg
    17521 atattcttca ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat
    17581 cacagttggt tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc
    17641 tattttattt ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat
    17701 cattgcttct tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga
    17761 ctggatttct ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc
    17821 gtcgtcactg actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa
    17881 atcaggaaga tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa
    17941 aaactttgtg atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg
    18001 ctgcagaatc aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat
    18061 aaatggaaca tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg
    18121 atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata
    18181 gtttccaatt gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat
    18241 catttcgttg tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag
    18301 ggcctagaga taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt
    18361 agaatacaaa gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt
    18421 tgtttattgg ctattcgact tactgattag ataccagcta cactcacaaa acatacccca
    18481 ttcttctagg tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa
    18541 ctttagtatg ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct
    18601 ccgatcattg gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta
    18661 gctattggca ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca
    18721 tatcctgttg gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat
    18781 acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca
    18841 aatgtaccaa ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt
    18901 gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa
    18961 agccaaacaa gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg
    19021 acaagattcc aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa
    19081 tgagctgggc ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca
    19141 aatgcgatga caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca
    19201 cacacaccat ctccaagcaa aactaggatc agtgtcccaa taaactctcc catgatttga
    19261 gtcatatcac tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc
    19321 aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca
    19381 gcaattgcat cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga
    19441 tgatttgtac gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca
    19501 taacgtaacc gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct
    19561 aattcaaaga tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct
    19621 tcctctaaac cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta
    19681 gttggatcga agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct
    19741 attccgtatt cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta
    19801 cggtaatcgg tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa
    19861 gaactacctc tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc
    19921 aacacgtctt ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg
    19981 acaactttat tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa
    20041 ccagaattac caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat
    20101 gtgatatttg cttctggata gcgatgattg atcacatcca atagataatc tacatcttct
    20161 tgtgtgactt tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa
    20221 gtcttgtttt ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag
    20281 tatgtgggtt gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt
    20341 ggacgcattt taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa
    20401 ggaccactcg tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg
    20461 tcacgagctt tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt
    20521 ttacttacta gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg
    20581 gcatcattgt tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa
    20641 aacggttctc tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct
    20701 gtcacatttg ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag
    20761 gttgttgctc cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt
    20821 ggggcaatac tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca
    20881 aatgtcttca gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct
    20941 tctgcaaaat cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact
    21001 gccacaccgg caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc
    21061 attttttgaa tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc
    21121 aaatgcttgg gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc
    21181 ttcctccatt tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt
    21241 atctttccaa taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc
    21301 caagttttct gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata
    21361 ttcattgttt gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc
    21421 ttgcatcgta tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat
    21481 gatatcttct tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc
    21541 ccaataagga gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt
    21601 agagcgtttc gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct
    21661 cagccattga atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt
    21721 tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc
    21781 accggtattc atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc
    21841 gaatgccatt tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc
    21901 ttcgcttcca taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac
    21961 ttttggtaac atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag
    22021 ctgatggata ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg
    22081 tccatcggtc agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt
    22141 ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga
    22201 gaaataggca tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt
    22261 ttttaagtta tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat
    22321 tggacgtcct gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat
    22381 cccagcaatc tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg
    22441 tacagaattc cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg
    22501 cgtgaactct ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg
    22561 tgagctagtt gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc
    22621 ctccattata tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag
    22681 aaactgtcaa aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt
    22741 ttttaatcaa ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc
    22801 gtaaggtgac gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa
    22861 tgtgatcacc aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat
    22921 tcccctcagt tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc
    22981 taaatcgatt ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt
    23041 ggcatactgg atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg
    23101 ctgcaccgct gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt
    23161 ctgctgctgt tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa
    23221 ccattcattg ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa
    23281 acgacgagga cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt
    23341 tggtaacttt ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga
    23401 aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt
    23461 tcgactaaga tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata
    23521 gagtaatttc ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt
    23581 cgtaatcccg agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa
    23641 tacacggtcc aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg
    23701 tcgctgatct tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc
    23761 ttctccgtat aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa
    23821 ttctttgatc tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac
    23881 gatggatact tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg
    23941 caataaaaac aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg
    24001 atactcattt cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc
    24061 ttccaaatat tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat
    24121 agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt
    24181 tctttctaat aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat
    24241 ctggcacacg gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc
    24301 atccttcttc tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt
    24361 ggagctgtgc tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc
    24421 tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa
    24481 aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc
    24541 cgactggacg ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat
    24601 ttcgtgctgt acggacagcg ttatttacta gagaaacata cattttttca tttgttggac
    24661 gaaaagttac ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt
    24721 agtcatctac ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat
    24781 tgtcggggcg agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg
    24841 tctctacaat agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg
    24901 aaagcccttg atttttcatt tcctttacta acggttctgt catttcagga tctagataac
    24961 gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc
    25021 cttcagctat ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca
    25081 tgatcttttg ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg
    25141 attctgcaga aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa
    25201 aatccaattt ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct
    25261 tttctgtgta aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa
    25321 gttgtttttc actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc
    25381 ctaacttcga ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat
    25441 aaatagccgc atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc
    25501 ctctttctta taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac
    25561 tcgctttcaa ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt
    25621 gttctaggtc tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga
    25681 agagatgttt caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct
    25741 gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt
    25801 tagaatagac acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat
    25861 tgatgagcag attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct
    25921 gaaaaagctg ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag
    25981 aatggtactt cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact
    26041 gtacgtccag ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca
    26101 ccgtgacaaa cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca
    26161 agatctattg cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct
    26221 gcataatcat ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg
    26281 aaatcttcat cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt
    26341 tctaaaggta catatccgcc tttaggactg acaaaatctg catcgatccc attttttact
    26401 aatacatcat aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt
    26461 tcgtactttt ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat
    26521 aaaaattgcg ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa
    26581 gctctttata tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata
    26641 atttctctgc ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat
    26701 atttcaatac ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga
    26761 tctttccttg aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag
    26821 aacgagctat tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac
    26881 gtaatcgttc aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt
    26941 ctggtgagaa acgataccct acgatgaatg gttttttagc atattgttca accgctgaga
    27001 aaaccgttgt taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt
    27061 gatcctctcg tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc
    27121 catgcaactc gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg
    27181 cttggattgt ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac
    27241 gttttgctgc aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg
    27301 tcattcgtcc accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt
    27361 tcgctagctc acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc
    27421 agccttttcc gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag
    27481 aacgttgttt ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt
    27541 gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa
    27601 ttgattgatc aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg
    27661 agaatcatct atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa
    27721 ttttattttt tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt
    27781 gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact
    27841 tccttagcaa attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta
    27901 actgttttga cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg
    27961 gcggaataga gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc
    28021 aataaaataa aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat
    28081 ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt
    28141 tcaattggct tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat
    28201 tttttgtcaa aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag
    28261 tagccatctt caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa
    28321 tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc
    28381 tttccacatt tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt
    28441 atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    28501 agttcctcat attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt
    28561 ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa
    28621 cagcttctca tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact
    28681 gtttattcgt aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta
    28741 ttataggatt tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt
    28801 taaccgtttc tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg
    28861 ttttgacatt tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc
    28921 aaataaatct actccatctt tgatagcttc cttcatctgc tcaatatgct gtttcaaata
    28981 gtcaatgcgg taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag
    29041 tccgttttca gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat
    29101 tctcaaaccc ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt
    29161 aattcctcca gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac
    29221 tgcactagac atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc
    29281 ttcatcacct acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata
    29341 cgctggataa tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc
    29401 aattgttttt tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat
    29461 catacaaccg acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact
    29521 tgctataaac tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc
    29581 ttcaattact cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt
    29641 catccaatat tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta
    29701 aaactgaata agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat
    29761 ttcataatgg gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac
    29821 ttgatcataa aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat
    29881 tcttgtccat gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat
    29941 gtcttcttta aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct
    30001 gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca
    30061 tttttcttta ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc
    30121 atctacatcc catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc
    30181 ttctggaaat tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt
    30241 ttcttatgac tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg
    30301 gtcggatcat atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct
    30361 ttacgtactt tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact
    30421 gtatcaatac cgatatggat cagtatctct gctccttctt cactgactac cccaattgca
    30481 tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc
    30541 gttggaatga tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact
    30601 tcagataaat caattatttc acctgctgca ggagtactta acactatatc ctttgaggat
    30661 tcttcttcga tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc
    30721 gctaagaaat aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac
    30781 aaaatacttc ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca
    30841 aatatagctc cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca
    30901 taaacagcag gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt
    30961 tttactttct tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct
    31021 acggcagtca tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc
    31081 attactggta caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg
    31141 ataacagcac cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc
    31201 agctgacttg cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg
    31261 atgatacaaa tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc
    31321 ttaggctcaa tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta
    31381 taccttgcta gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca
    31441 ctgacaatcg taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta
    31501 ttgaactttt ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca
    31561 gctgctgcat tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta
    31621 cataaggcga gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca
    31681 aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc
    31741 tttccactag atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca
    31801 tttactgcat ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca
    31861 ccttctagtt gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt
    31921 aaccttgttg cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca
    31981 atatctttcg ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta
    32041 gcaatccttc ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta
    32101 ccccttataa tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa
    32161 tcactcactg cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa
    32221 aaaacacgct tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg
    32281 atttttgttt ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga
    32341 agcataacta ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg
    32401 atttttattt gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct
    32461 tcaactatgg tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct
    32521 agaacactag gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc
    32581 aaaattgcat catattccgt tattttgttt gattgatttt tgaaaagata cactggttct
    32641 atccggcatt ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc
    32701 aaaatactaa ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc
    32761 aacgcttcgg caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga
    32821 aaatgtattg ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc
    32881 ttttgtacaa aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg
    32941 atatgggtta gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa
    33001 cgaataatcg tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca
    33061 ttaatcatca aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg
    33121 gaaaaagaat agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata
    33181 aataatgtat acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg
    33241 agataccgag aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga
    33301 acctctttta atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt
    33361 tgaggcaacc ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt
    33421 tttgaatttt cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat
    33481 ttaggattgc gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt
    33541 gaaagaaaga aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag
    33601 tacgtcacaa tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc
    33661 tcttgtatga tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt
    33721 ttatttgact ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt
    33781 tggatcgttt tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga
    33841 ttcttatttc ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta
    33901 cacctcctct ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca
    33961 gaggtttaaa aatcatttga agaaagatct atattcaagg tttttaccat tagactggat
    34021 aatttgttta taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc
    34081 attttgtcta tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga
    34141 tacgatatcg attggtcccc accataagta accaatgatt tggcaaccat cttttattgc
    34201 ctcagcaatt tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg
    34261 attcaattca tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa
    34321 ttcataacgg tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca
    34381 actccaaggt tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc
    34441 agtatctacg cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc
    34501 aatataatct gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc
    34561 acagtcttct tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac
    34621 atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc
    34681 acagtcatat gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt
    34741 tggatcgatt ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca
    34801 ggcttgataa atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg
    34861 gaatgtaacc aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa
    34921 ggtcatccaa tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc
    34981 aaaaaagtcg attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg
    35041 ggtttcataa tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat
    35101 tagtctgtca taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa
    35161 tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg
    35221 aatatcctct tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga
    35281 ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg
    35341 gttcatgatc ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc
    35401 accttcacat tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc
    35461 ctgatccata actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt
    35521 catcaaaaca agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact
    35581 cttcattttt aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc
    35641 gttaaccatc taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta
    35701 aatcaacttt gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag
    35761 cttcacgtgg aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt
    35821 gctggtcttc atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt
    35881 catagtctcc ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca
    35941 tataaaaaac agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg
    36001 gattgactcg aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt
    36061 gcataggcgt cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt
    36121 ttgtaaatcc agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt
    36181 ccgcgatatc atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag
    36241 gggtgccttt catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca
    36301 catcgtcatt tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac
    36361 tattccatcc ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag
    36421 agatactcaa gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt
    36481 gttcgaattg gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga
    36541 tttcggtagt tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg
    36601 cttgattcat ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat
    36661 ccggttcttt gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga
    36721 tccagaaatt catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat
    36781 caggttgacg cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc
    36841 aagcagaacc actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac
    36901 gccaaatata atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg
    36961 gatgttcatc agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct
    37021 ttgcttcttc aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt
    37081 gataattact gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc
    37141 aaattgcatc aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat
    37201 ctccaatacc atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg
    37261 aatggtgcca ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt
    37321 gcttaatcat ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg
    37381 caccttgtta tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt
    37441 tttttgaaag gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg
    37501 gtgttcactc agctgtccct tggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    37561 ttttcgtttt tttctctaat actcaggcca gaacactgat ttcacaacct ctttataaac
    37621 ggtgttcact cagctgtccc tcggtattag ctcaaaaaac ggcaaaatat aagaaactat
    37681 tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc
    37741 acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat
    37801 acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca
    37861 atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc
    37921 gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc
    37981 ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac
    38041 aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg
    38101 gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc
    38161 gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct
    38221 ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc
    38281 acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt
    38341 tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc
    38401 attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc gatgaagatg
    38461 ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac
    38521 caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg
    38581 atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca
    38641 tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc
    38701 aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc
    38761 attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca
    38821 attggataac gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc
    38881 gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat
    38941 aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg
    39001 gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct
    39061 aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt
    39121 tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc
    39181 gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta
    39241 caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa atcaatagtg
    39301 tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg tatcgacagg
    39361 attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc cttttgtctt
    39421 cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg ctcgccgata
    39481 cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt tttattcaat
    39541 cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa aaattgaaag
    39601 tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg tagatagttt
    39661 caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct ggtgaaaact
    39721 tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg ctgagtacaa
    39781 tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca attttttcta
    39841 caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt gttttttgta
    39901 tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact gattccatta
    39961 tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa agcaatagtt
    40021 tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaaacggca atgaatgccg
    40081 ctcttcttat ttcgattctt ttataattct gcttctgatt catctttggt tagtgtgtgt
    40141 ctaacactag ttacgccaaa tgtttgatcg attttttcct tcaacgcttc gattgcctgt
    40201 gcgccacgtg ccgcttgttc agcattgatg gattcaatga ccagcaccgc tgcttgtgtt
    40261 tcttccgtca gcattgttct ttgggcttca cgccagttga gacagcgaca gatcgctcct
    40321 tgttcatcat agtagatgat ttcttccgga agtgccggag catcggaatc tgcacctaaa
    40381 ggcaggaatg attcgccacc ttgggctttt cctaaatgca gatttcctgc gatcttttcg
    40441 atatcttctc cgccgcaagg tacgccaaaa gacaaggaaa tactattata gatatctaca
    40501 agcggattga ttgggaaaaa ttcccgttct tgatttgcac gtttcagtaa agcttcaata
    40561 gaagaacgtg cacctttttt agtcttgaat gtggtaaatg cctgacgcca ctgagcaatg
    40621 acttcgtttt ggctgaatgt ttcttctgta aggtaatttc ttgcttcttt tgctgcctca
    40681 ttcaataatt gatgatacgt ttcttcactt tcttttgtta cgtggttatc gataccattt
    40741 acggataaga tttcgatggt tgcttcagga aatacttccc agaaattggt atccactatt
    40801 acttttttca tctgttcaca cctcttgatt agaataattg cttgatgaat agatagtctg
    40861 tttggggatc atctcccata taaaaaacat gttcgctgta tttgataaat ccttttttac
    40921 gataaaaagc gagcgcaaca tcgttgtgtt cccaaacacc gagccaaatg gctgtttttt
    40981 gcttttcttt tgctttttca atagctgtat ctagtaaaaa tgtaccgata ccttgatgtt
    41041 taaatttttt tcgtacatag attcgctcga tttctaaaaa atgatcagcc atttcctcag
    41101 attgactcgt tcctgtattt agtttcaggt aacccgcagg ctcttgatcg atactcacta
    41161 ggaaaaaatc ggtttcttta tcttttagct cttctgtaag tttttctagt gcataagctt
    41221 gtgtcaagta atcagctaaa tgctccggtg aattatcttt tccaaatgta tctgtaaatg
    41281 tttcgatact aatttgttgt agtaagtcta catctgaagg cgtcattttt cgaatttcgt
    41341 atttattcac ggcttcttcc tttcaataga tccttttatt gccttttttg acaaaattcc
    41401 aatcttcgct aacattcttg catactttat ttaaaagata ttccaattgt tttgcttctt
    41461 cgtcagaaag accatttaat gcgactgcat tggagtagtt gttttctcga atgatgaaag
    41521 gatagatttc tgctcctttt ttagtcggat agagtttctt gattttttta ttttctttat
    41581 cttccaaacg ttcgatcatc ccattggatt ctaatttctt gattgctcgg gcagtcgttg
    41641 tacgatctac tttgatcatt tctgctagtt tttcttggat gattccagga ttttcgcaaa
    41701 tacgtactaa ataaagatac tgtcctcttg ttaaatcgac ttctttgaat tcgatattgc
    41761 taatagaatc tagcgctcgg gcaatcattc cgatgtctcg aaggatttca gccatttcat
    41821 cccaccttta ttttgtttgt aagactagtt tacctctttt tgttgcaaat gcaactattt
    41881 tcaataaaaa aatcaagttc ttttttaata actagtaaag tatattcttt tcttctttta
    41941 cgaaaaagct caatcacaat agatctggtt tttgttttct aaaataagta actgtttctt
    42001 cgttgttcgc ttttctgata gctttggcaa ctcttctata ctaaaaaatc ccatgttgga
    42061 agtttcatta ttctcaataa acttggcctc ttcaccgtgg atcgcacaag caaaaacaag
    42121 tttataatac tgaaaaagct gtggaatatc tttttgttta ttcgtatcaa aaactgctcg
    42181 caactgagta gtttctactg ttaatcctgt ttcttcatag acttctttac gcacattttc
    42241 ttcaggagac aatcctattt cagcaaatcc accaggaagt gaccattctt tcgtccgttt
    42301 atcttcgatt agtaggacct tgccatcttt tttgatatag gcacgaacat ctacttttgg
    42361 tgttggatgt ccttctccaa tcgttgtgag cgcttttatt tcttcgaaag tttcttttcc
    42421 tatttcactg atcagttcca atgaaatcgt ccggatctcc tcaaaacgct ctttatcaaa
    42481 tacatctttt ccataatata gaccagcttg agaaagtgcg agtagtcttt tgtattgagc
    42541 caacttcttg ttcattctct tcccttcttt tttagcaaat gtatgactat gtaatcaagt
    42601 atagaagact ggtttaatta cggcaaataa atataatgaa atctgcaata aataaaagaa
    42661 tgttaaaaaa ctcgagaaca aaagacattc gacttctctg gggctgtgaa taaatggcgt
    42721 tcaaaagctt gcatctgcgg taataagccc caccaaacga aaaatatgac gagctattct
    42781 tgtctggctg ttcttattaa ttgaggcagg acagcttttt cacaacctcc ccactaaacg
    42841 gtgttctcct accaactcct cggtcacaag tcacaatatt catcaaacat gagaagctgt
    42901 ttttttgaaa ttgctccttg tgactcggag ctgaacgaca ggctcacgac ctctcggtaa
    42961 acagcgttca aaagccaatt tttcgatatt aagccatttc tttgttactt attgtcattg
    43021 atttcttttt ctaatttatc caatagtgga gtgtaatcca cttgagtata gtctgttaca
    43081 tcaaattcat atagtgttcc taactggaat ttgtcatatt ttcggtcggc gatgatcttt
    43141 ttgaatgctt ctttcgtaat atgattggtc cctttcgttc gatcttccaa tacgtcacca
    43201 tagtgataat gatccatgat atagctcaaa tgacgatctg attcacgatc acgtttgtac
    43261 cgacgttgac ataacacttc aaagtctgct gttaggcgaa tcgtgatcac ctgatatccg
    43321 tatctatctg ctaattcttg gagtcgtgct ttttgcttat cactaaaagg atactcggac
    43381 acaatcactt tctttcctgc ttccatatac aaatcaagtg ctttgtaata gaaaggataa
    43441 actttcgtcc tttcttgttc cgcccgttct tccaaattgt taaacccgat ggattctgca
    43501 tagtattcct tgatttcatc gagcgtgatg gcatacatat ctggaaatcg ttcccgtagt
    43561 ttattcataa gatatgtttt tcctgtacca ggactaccag ccagaagaat gaaatacttt
    43621 ttcatctcat ttatttccta agttaattgg actttccaag ttcataaaca gtccaatctt
    43681 atgcttgaca atttctttta ctgcttcatt tgctggaatc acattatggc gaagagactt
    43741 gttgatttct gtttcactaa aggtttcttt agctactttc gtccagttga ctaaaagctc
    43801 cgttccaaca ttgaatttgc ggatcccgtt attgatcaat tcaggataat cttcttcttt
    43861 cacacctgtt cctccatgga taactagggg gacatcaact actgcatcga tttctttcag
    43921 caagggaatg tttacttctg tttttgattt gaattgtcca tgattcgtac cgatagcaat
    43981 agctaatgca tccacgcctg tagcttctac aaatttcact gcgtcttccg gtcttgtata
    44041 taccttgtca ttttcagcga catggattcc ttcttctgta ccgccgattg ttccaagttc
    44101 tccttctacg gaaacaccgt gcgcatgggc atattctact actgcttttg tcttcaggat
    44161 attttctttg aaaggcagat gggacccatc atacatgaca gaagtatatc cgctgtcgat
    44221 tgcttctttg atatcatcaa agtctcgggc gtggtctaag tgcaagactg catcaaccat
    44281 ttcttcttct gcaatagctg tcattacatt gactaacaca tgcatgccga tatatttagc
    44341 ggtatcgaca cttgtttgaa taatgatcgg cgaacccatt tcttttgctg cgcctagcat
    44401 agcaggaagc atttctaggt tatgcgtatt aaaagctcca actgtaaaat tcaactcttc
    44461 tgctgttttt gttacttctt tcaatgttgt atacatcttt tattgccacc ttatcttttt
    44521 atttgtttcc tttgatcgtt acttgttttg caatttgtcg catctcatcc attttgccca
    44581 tgtagtaatc gatcccttga tcatcaccag cacgagcagc atctgcacgc ttctggttat
    44641 agaggctcat cagtttttta tacccatctc cgatcgtctg tttattttcc aaactctttt
    44701 ctaaatattg gatggcttga tcgttcacat acaattcaga aaatttaagt ccaacttgtt
    44761 cataaagttt cgctaattta tgcttatctt gggtagtgga aatctcttgt ttcaattgtg
    44821 taatctctga ttgaatcttt tcggtcgctt ctggcgatag ggctttttct tcttttgcca
    44881 gaactttttt cggttctttt tcttttttct taaaaaatcc aaacatcatt ttccctactt
    44941 tcaattaaaa tgctttcaaa actgggctta agatccaggc atataagagt gctgcggcta
    45001 ctgtatctac atcggttgct gtagcattga tgaatcccat cgaattaaag atcgttacta
    45061 gcaaagcggg caataatgta ataaagaaac catgagcaat accgccaatg atcgctcctc
    45121 ggcgtccgcc aactgcgttg ccaaatatcc ctgctgtccc tccggcaaag aaattggtta
    45181 gcataccagg taaaatcatc gctaagccga acatgggcaa agtaaacatg gcaataatcg
    45241 taccaatcgt tgtcgtaatg aaacctaaaa tgacagcatt tgggctataa ggaaagaaaa
    45301 ctggacaatc taaggcaggg atcgcatcag gaactaattt catagctatc cctcgaaatg
    45361 ccggcacgat ttcacctaac agtagacgta caccagctag caatacatac acacccacta
    45421 cgaattggat ggcttgtaaa aaggcaaaca tgatatagtt ttgatctcct gataattcag
    45481 aagcgaatgg ttcgcctgca aataaaacag tgatgatata tagcgggacc atcacgacca
    45541 tgacagacaa ataagtatct tgcagaaatt cgaaatgtgt cggcaacttg atatcttcga
    45601 tcgacttttt attttcgcct ttctcaccaa aaagcttagc aacaccggct tcaaataagt
    45661 acccgatcgt acaaaaatga cctaaagcaa tatcatttga tccagtgatt ttacgaatga
    45721 tcggttgcgc tactgccggc atagcaatgg cgaaacaagc tccgataaaa ccaccgacca
    45781 agatcaatac gatccctcgc agaccggcaa aatagccaaa gactgtcgtc atcgttgcca
    45841 tccataaaat tgcttgacca gttaaaaaaa tgtacttcca ttttgtgaaa cgagccaaaa
    45901 taatattgaa tacaaaaatg gctaagaacg tcagtgcaat atctctacct aaacccaaat
    45961 cattcattgc ttgaccattg atagattcaa tagaagggat gatcccttgc atatgaaatc
    46021 cttcagtaaa gattttcccg aaataagtta aacttccaac gatgatgctc gaaccggcac
    46081 tcaacacttg aaatcctagg agtgtcttta acgtgccaga gatgacttgt cctgctgatt
    46141 tcttctgcag tagtagaccc aacatagcaa tcaatgcaat ggtgatcgat gcctgagtca
    46201 aaatattttc gatgataaaa ttgataacgc ccatgttttt accctctttt ctttttgtat
    46261 ttagataact cccttatctt gcagaactgg ggtgattttt tcttcaattt cattttttga
    46321 aacaatattt ttcaaataga tcatcgccgt tttaggatca actgcgaact tttcgaactg
    46381 cgaacgaaaa ttttctgctg tgatgatcac atctggtttc atggaaactg cggaagaaat
    46441 atcacaatgg tccagtttgg cttctactcc taatttgttc aaaacatcct cagcagacat
    46501 ctgtgctgca aaactacttc ctaaacctgc accgcatacg aataaaatgt tcaatttgtt
    46561 catctttgtt ctcccccgta tcatttatta tttctcaact actgaaaaaa gcagttgttt
    46621 gaaatcttcg atatttgtgc attcaatcaa tcgatccaac ttctcttcgt tgttgatcaa
    46681 ttcaattagt tctttcatga tgttcagatg cgagtaggag tctactgctg ccaaacaaaa
    46741 aataatcttt actggatcca tttcaggatt accgaaatcg attggttgct caatcgtagc
    46801 tacgctgact cctaatttat tgaccccatc ttctggtcgg gcatgggcta gtgccatgtg
    46861 tttgccaatc acgatatacg gtccgtaatc attcactgca cgaatcatcg cttcgacata
    46921 gcgctcttcg acaatgtttt ctttaaccaa tggtctagca gctagttcaa tcgattcctc
    46981 ccaagtagtg gctttttctt tgatcatgat attgctgtcc gtcaaaatat cttttaacat
    47041 aggctggatc tcccttttgt tgatttctaa attgtttgtt tcgaataatt tttctaactt
    47101 gccatagatg attcgatcta ctcggccacc actttctttc atgacatcta ttatttgagt
    47161 gaataactcc gtcgattcat gactgttagg ctctaaccgc tgtagttctt catttctttt
    47221 caaaaagtct tctataacca agatattcgt ctcagttaaa atcggatcga caactaaaag
    47281 cggcttattt cgataattca attgaaaggt gctgaaaacc agattgacat ccaatttatc
    47341 tactaattcg atttcttttg aacttaaaac tgcagtaatc tcgatctgag gaaacttttc
    47401 tttcagattc tctgctaaca gatttccagt agccatccca tggttacaga caacgactga
    47461 tttataaaca taattcaagt ctcgcttgat tacactggcg atcgtcgaaa agtggatgac
    47521 aagaaaagct atctcgtcct ccagtatctt gccaccaatt acttcttcga tcgtaggaac
    47581 aaaatgagtg actgcgcggt agattgggaa ataatttgtt cggatgtttt ctttcaatgg
    47641 attcatgact tggatatgat tcttgattct tacaatcagt gcagccatgt gcttatagag
    47701 attttcgaat aaagcttctt cctttagatg gaaaggtacg tgtgtctcgt tctctacaaa
    47761 ttggatcaat tggatcgtta gtaattgagc ttgtacccac tcgattgaat tggagatatc
    47821 tctagcattg aacgtctcaa tagtgaaacg aagataattt ctttctactt gcgggacatt
    47881 gtccaattga aattcctcga tcatgtggtc aataaatgca gaaaagctac tgtcttttat
    47941 ttctgtattt ttccaattaa tggcagagat aaattcatgt ttcctgattc gctggatcca
    48001 gatcaatgta aaaagcagga tttgtttttt ataaatatca tcttcttgtt tcaacaactt
    48061 attggaatag atctcctgta tttttttgat ttccaccaaa ggaaaataat ggagaaagat
    48121 tttttgagcc ggtgttaatt gtgcatcttg attcataaag aagtcggtca ttcccagatt
    48181 ctgattgata aaatcataga tcatcgtacg gatagatcgt tctgaacctt tataaaccag
    48241 tccttgcttt ccataactga cgatctctat cccataactg accagtgttg ctcgtaaccg
    48301 tctcatatct tcatctaatg tacttttaga gatttgaaat tcttcttctt ttttgtttag
    48361 atggatcggg attcggctgt aagcaacggc taaaactaga tcaaaaatac gttcgcgccg
    48421 attcaaaaag ccgtctttca catcttttat ttcagtttct aatcgagctt gcttttcctt
    48481 ttccagcttc aacaaaaaaa ctttgttgcg aattgtacgg atctccggaa gctttttact
    48541 gctcaaccag atattcgctt caagaacttc ctgacggatc gtccgctgac tgacattgaa
    48601 ctcacttgct agatgttttg cttttactgg atattgggct gtcatgattc tgtgaagtat
    48661 ttcaattgtt cttgttttca tagaaatgat ctccttctga tttcaagtat atggttccgc
    48721 ttacataata aaaagtagat atttctgcca cttgaagtgg aaagaaagta agcaaagcaa
    48781 acaaaaaata agccatatgc tttttcatct cctcaacgaa aaagacatac gcctattatt
    48841 acaaaaattt tgcccatcta tacaaaaaag aggttgtgaa aaagctgtcc tgcatcaagt
    48901 aataagaaca actaaactaa aatagtgctt cctatttttc gtttggttgt tcttattatc
    48961 gcagatgcaa gcttttaaac accatttagt ggagaggttg tgacagagat gttcagcctt
    49021 gagcgataag aagcgatttt gaaaaatcgc ttcccatatt ttacaaaatc gtgccttatt
    49081 gccgataggc tatctctggc acactgttta ttcagaaaaa ggagctgcga caaggctttt
    49141 gtcacaactc cgttcataaa tatttcctgt caatccagca taatcggctg aataaaatcg
    49201 gcttgccatc ttttttcttt ctcagaaaaa tcaaaatacc atatctgaga aaaatactgt
    49261 cgttgtgtct tacttcctga gatcaatcgc ccatcttttc gatcgactga ataatcacag
    49321 catctgaaat caattcggat ggcaaaacta ttttcgtgga tctgtcgata atttcctact
    49381 tcctgaatag ttattttttt agtatgattt cttacaccgt ctgctttgtt tttttgcaga
    49441 actttctgat gttcggcaaa cagcttgtct gtataacagc ttctggcttt ttcaggagtt
    49501 tcttcttccc aagcggattg gatcttcatg aatatctttt ggatttccag aaactgtgct
    49561 ttcttttctt tagccgttcc tgacatgcgg gacaatatct ccgctttcag ccttttcttt
    49621 tgtcttttat tcttgacgat agaaaaagca gaaacccctc ctactgacaa aacagctaat
    49681 gcaagaaatc ccgtttgctc ataactagaa ggacctgtac ctggaccata atacccataa
    49741 gaatctctgc cgtaagaacg actgccaaaa taactgcccc catatgaacg accaccaaat
    49801 gaacctctgc ccgaacttcg accgctagat gtacctccac catgaccacc cgcgacagcg
    49861 aatccttggg tcggcagagc aaacaaacat aggcccacta acaaaatgat tgctaaataa
    49921 ttgatttttt tcattgtctt cctcgttatc ttttattaca aaaaactatt tttatgatag
    49981 ccttgctaaa aggaagagtc aataaccaga tgcataaact gtttttttag caattttgtc
    50041 ttatttagtc attgagtaac atcggacaaa tgtatgtgtc ggcagtccat aattcctcta
    50101 tcttcagcat gaacaagctt gcagacaaga gatctgcgct tcctccagga gagatttggt
    50161 tttcctgaca aaagtgcaac agtgctgtaa atggacgatt tggtaaagca ctttgactag
    50221 cagcatgagc aagtttttgc atttccacta acgttttctg atcactgcga tgcaatacgc
    50281 atgtgtcatc tagcgaactc atcaaaacta acaacatatg catttgtttc tgtacatatg
    50341 aatcattatc tagctcctta gctgcttgga ttgcaagcct gatatgcgga taccctaata
    50401 tcgcttcttc atatgcacca tttacgccat atttttgctt caccctatga ccatgagtgg
    50461 tttttttatg acgatatttt gtatcaggaa aagcagcaag cttgctgaca tcatcaagta
    50521 ttttcgataa agaaaaaggc tgttccatac tgattttttg tgcaatgaca ctgatcaata
    50581 gccccattgc ccaaatcgct cctttatggg tattgatgcc atttgtagca atcagcattg
    50641 ccttttccgc ttttcgccca atctcagcaa ttctttctct taactcctga tcaatagcat
    50701 gatgccatgc tatttgagcc atttcttcaa aaaaaggagt tagcgtctta gcagacttaa
    50761 gaaataaagc atagtccata tcggaatgtg cacccgcatc cagtgcatcc actaatccag
    50821 gttttggact taatgttact tctgcgatca atgcttcctc taccaacgca gcgagttgct
    50881 gagtcactgc tttttttcgt tcgtcattta tcatgacact cactccttct tcctactatt
    50941 acttgctgaa accttttttg atagctaccc aaatgatatg aagtaaccag cttaaagcaa
    51001 atgcacaact tgccgtcatt agataagcta caagaatcat gactcctgaa ggtaactgca
    51061 gccaactatc tttaaacaat aataacagta cttggaaaac aaatacattg gctaaatacg
    51121 tacgataagc ataaacagac aaccatttga acaatggcaa ttttggcgaa tcgtttacaa
    51181 tcattttgga agcgcctatg aagacagcaa agatgatgac taaactgtat aaactttgga
    51241 tcgtattcaa gtaaggtgca ttcgcaaagc tcaagtctcc tggatgtgcg agcaatgaac
    51301 ggacagaaaa gaacgcgata actaaggcaa caggtaagaa aagataacgt actctgtcca
    51361 aatattgata gactttttcg tgataaacaa atgcggctac cccataaatc atgaaactag
    51421 caacaaaccg atctaataag taccagctgt catgataagg cccagtcaat acttgtgttt
    51481 gatagaacag ataccatgcg acatataaaa tcgttgaacc aatcaatact ggccatacat
    51541 attttttatt agcaaatact ttgtaaccga gccaaacaaa aaacggcatg aacaattgga
    51601 tctgcagcat cataacggta taccataaat gaggcgctcc atcacctgtg acatatttca
    51661 gtaaaaagct tgcagtatcc gtatacggag tttgttgttg gactgctgga aatagccaaa
    51721 gataagccgt cgtccataga agatacggaa cgactagttc aaaaaacttt tctttcaaaa
    51781 attcccaata agacaaatgc tgacttgttt ttaccatatt ataaacaatt gcaaaaataa
    51841 acattggtgc tgtgtattta acgatgacgt aaaaggtacc aagaaaagct gtatttgttg
    51901 cgctgtggct gtttgccaaa gcaaagctca atatcgtttg catcataaca gccgttacag
    51961 ctgatacttt ctggaagtca cttacgttga ttttgatttt tttgttttca aacatctctt
    52021 tctctctctt tttctttatt accaaagttc tttggttaat ttaggtcctt cgactgtttt
    52081 caatagaatt tttttctctg gctctctcaa atactctttt agtgaaaaac cgccttgccg
    52141 agtcactact tgggtatcga attgctcaaa aaaagcagga tgcgattgaa ttacctgaat
    52201 tggcaattca atcggtgaat ttgcgtacag cagcaaatca aaatcactgg tttcttttac
    52261 tgccggaatc ccagtcgata attcaaatcc taagctgccg ccgattcccc atttacatcc
    52321 tgataatatg tttctagcag catgaaattg ctgataaaca ggataatcca tgattttctc
    52381 ttgtctatac gattcttgtt cgatgatgtc ccacggatga atgacttgtt tgacggcatg
    52441 ttttgagatt tctgttgacc atctctgatt gcgggcaggt ccacgtaaac caatcggtag
    52501 tatttctgcg tgatccgtcg acgcacgacg aactgttccg aacatctttt cagttagaac
    52561 gacccattca ggcaaggtga acccttctat ttcatgttta tcaaaaagga caatatcatg
    52621 tgcttgaatc attttttatg catcccattc ttcgtccatt ttggcacgga ctttatttgt
    52681 ttcgacacgt ccgaatgttt tggctgtttc gtttgtgtat cgatttgtca gcatcgtgtc
    52741 atttgtattg cggacatctt gaatggcttc attgattgct tcgatgactt tgctgacatt
    52801 ttgttctgaa gcagcttggt cggtgatatc ttctaggaaa tgatagatcg ctcctagttt
    52861 tgtataatta ttgatatcgt aagcgatagc tggaactttt tcagcagctg cttcgatttc
    52921 agcaaccgaa cgttttgtca cacgtgcagc actttcttta gacatcgctt ggacggtaat
    52981 ttctttattg tttaaagcaa tcaagcggct tgattgtaat ccatgtgcta aaaatgcacc
    53041 tgaaatagca tttcctgcaa tcaatccaat aactggatgt ccagcttgac ggagttttgc
    53101 ataagcagca gcactattag ccaaagccac atggataccg atcaattctt cgttatagcc
    53161 gtatgcttgg cttggtacat cgatcacaac gatgattggc cgtttcactt ttttgtcttt
    53221 atcttcttcg taaacgtgat ccaaaatttg tcccatgcgg aaaccttcaa ctaaaccaac
    53281 ttcaccgttt cgtacacggt acatcgggtt tttaggatca ggtacgattg atgtcacgat
    53341 ggcttcttct atgaatttac ttgatttacc ataatacaca gtactaatag aagaagttgg
    53401 atttttcatg ccagttagtg ccgcaaacca atcgtaacca acacttgctg cagctggttc
    53461 atttccttct gctacttctg gtaagtcaat tgattttgct tgatggttag caattgcttc
    53521 attgtaatcg tcaatggcca ttggttttga aagatctaaa gaatcaagta atgataaata
    53581 aaatgctgag ctttcactgc gatgcgtgtc ttttcgttcg tccattgctg cgatgacagc
    53641 agctttgatt gcttctacac tatcagtcac taattcatca atgatttttg attctacccg
    53701 ttgacgagat cctaatgtat tccaaatcaa gtctttgtca ctagaatcaa attctcgaac
    53761 gcctgcttct tgttcaataa cttctggtcc attcaaaccg actctcgctt tttttgttgc
    53821 gattaaataa ctcattaatg ctgaagtaat cgacataccg ccaaatgatc ctacacgtcc
    53881 tggaatcaaa ccgatgactg gtacatattg ttttagagca atgattacat ttccgatttc
    53941 tgaaatagat aacagtccgt agtttgcttc ttgcaaacga acaccacctg tatctagtac
    54001 aatgactgga aagatctcat ttcctttttt attttcttct aacacatgtt caagtgcagc
    54061 aacgattttt gcaccggaaa cttcaccgat accgccacct tggaattttc cttccatagc
    54121 gattactact gcttgtttgc cattgatcgt gccacggctg acaacgatcc cgtcatcact
    54181 ttcagggacg attccttgtg ctggtaaact tggcgcgatg atttgatcga atggatcaat
    54241 caattcacgt ccattgtcaa gtagagcgtt cactcgttct ctggcgttta attctacaaa
    54301 actatttttc atctgaaagt tcctccattg cttgcgtcaa tcgtaggtac acaactcctg
    54361 gtgtagcacc gaagtcattg atcacgatat ctgctgaaat tggataacga tcaaaaaatc
    54421 tcgttaatac attgttccat acttcgccga agccatcgct tcctgtacaa atttttacag
    54481 tagattctgt tttttctgtt ggaaacatca agatttccaa atctcctgaa ccgaccactc
    54541 ctacatggat tttcttattg atcggctgtt tcgttggata tttataagct aattgttcca
    54601 tggatctctc ccctttttgt aaaacagagc atacacgttc tgcttattcg tctttcttct
    54661 tccatctggg taaatggcga caaaaggttt ttctcgccaa ttttatgacc aagttctgaa
    54721 tttcattggt ggttcataca ggccatctga ccattcgatc aaatcatcga tggtttgtgc
    54781 agctaataat gaacgtttcg cttcactacg tttgatgcct agatcttctg gtaatttcac
    54841 gatgccggct gcacgcaagc tatctagtga ttctttcgtg ctagtcattc caagaggtgt
    54901 aacaccgcca attgctgcaa ttgctgcttg tcgttcttct tttgtttttg ctttgtacaa
    54961 ataagcaatt ccttcttctg tcacgatatg tgtagtatct tcgctataaa tcatcactgg
    55021 tgcattggct aagcctgctt gtttttgaac agctagtgca tctaattctt cgacaaagac
    55081 tggtttttta tttgaaccat atgtttcaac catttgaaca actaattttt ggcctttccc
    55141 taatggatca tcattgtcgc gaagtgattg ccatgctggt gtagaatgac gacgaccgcc
    55201 aggattatgt cccatgttag gcgcaccgcc aaaaccagat aaacgtcctt ttgttacggt
    55261 agaagagttt ccttcgtaat ctaattgcaa ggttgaaccg ataaacatat cgatggcata
    55321 ttgtcctgca gcttgtgata atgttcggtt actgcgcatc gtgccgtctt taccgacaaa
    55381 aaagacatct gggcgagcag caatatattt ttccatacca acttcgccac caaaactatg
    55441 gatgctttca acccaacctg attcgatggc tggaatcatc gttggatgtg ggttcaatac
    55501 ccagttcttc gcgattttcc cttttaaccc tagtgattca ccataagttg gcagtaacaa
    55561 ttcgatagct gctgtattga atccaatacc gtgattcaaa gattgtacgc cgtgttctgc
    55621 gtagattcct ttgattgcca tcattgccat tagaatttgc aattctgtga tgttttgagg
    55681 atctcgtgtg aataaagctt ctaattgata aggtttatct gctttcacga caacgtctac
    55741 ccagtcacca gggatatcta cacgtggtaa agaatcgaca acttcgttga cttgaacgat
    55801 cacgatacca tctttgaaag ctgcagcttc gatgattgtt ggtgtttctt ccgtattgaa
    55861 acctgtgtac aagttgccgt ctttatctgc tttatctgca gctactaaaa caacatttgg
    55921 aatcaaatcg atgaataagc gtccataaag ttccaaataa gtatggatat cacctaattt
    55981 cattgtgccg tcttcaatca tttgggcgac acgcaagctt tgtgcaccag cataagagaa
    56041 atcgatcttg cttgcgattc ctttttcaaa aatattcaaa tgttctggtc gtgagatact
    56101 agacatgatc aaatgcagat cattcacttt ttctgcatct actttttcaa gactttctga
    56161 aagaaaactt gcttgttttt gattgtcgcc ttctaataca actttgtcgc cgggagcaat
    56221 cagtttttcc attacttcta cgattttttc agtttctaca tacttgccat tgagcaagtt
    56281 gctgattttt tccaatcgct gcgctttttt ttcgcgttta gaagcccagt tgcggtttgg
    56341 tttttccata attagtcatc tccatttttg atatagaaac gtggcgtcat caataccatg
    56401 ttggtctaga ttgatcgtac ggatcccttt atctacacca aattttgtat gaatcagctt
    56461 tgtcaaagta ttgccggggg gaaattcaat cgtcagatcc attcctaatt cttttgccaa
    56521 gtccatcatt tggttccact gcacagggaa agctaggttt tccaacaaat ctttacgaat
    56581 ctcttctgcg tcctgagtgg ctcttccatc cgtatttttt atatacaaac atctagcatc
    56641 agatagatgg acagactgca tatacggctt gaatttcttg accgtatcat tcatcaagag
    56701 acagtgagat ggcacaggga ctttcagcag cttcgctgtt tgcgcttggt gccgtttggc
    56761 ttctgccaac gtaacggata aaccttcgat actcccagat aacgtatgtt gcatcggaca
    56821 attttcattt gacagataaa ctggatggtc aggatcaaat gtttttgcta caatttcctc
    56881 agcttcgctt ctcgtcacac ctactactac gcccattgca taaccgcttg gataagcttc
    56941 tttcattaag actgcgcggt gatagactag ttccgctgca ttctcaaatc tcaaagatcg
    57001 acaagcaaca gctgcacaaa aagcaccaat cgaatgtcct gctgcaatat caggctgaat
    57061 accgtttttt tccatttcat ctaaataaaa tactgctttt gtcagtaatg ctagctggat
    57121 aaaaaccgta tcttgatagt tttcttcttt atcaactaac cgataccccg tcacattttc
    57181 taaacgttcc atgtacattt cagggatatc cgctaacatc cctaacttct gactgccttg
    57241 tccaggaaat aagaaacctg tactcatgag cctcgcctcc tcagcactta ctttttccac
    57301 tgctatctta gtcaatttct gctataataa aaaatgccaa aaaaagaaaa aaccataaca
    57361 aaaaggtatg gatgggatgg ataattatga aattgcttca tctgcagtat tttaaaaaag
    57421 tattcgaaac aaaaaatgtt acccaagcag ccaaagaatt atttatttct caacctgcct
    57481 tgagccgtgc gatcaaacat ttggaaaacg aattaggtgt ccctttgttt taccatagcg
    57541 gcagaaacat tgagacgacc gtttatgcag aagaatttta tccatatgca gtcaagacat
    57601 tagacacatt agaagaagga atcaaattat tgtcttcggt caatgagaag atcgttactt
    57661 ccgtcatatt gtatttagag gtagcctctg tttctattcc gaacttggtc cgaattttta
    57721 tggaaaagca tcctgatatc caattaacga tcatgcaaca tcatttacca gatgatatta
    57781 ccgatcctgt actgtacatc acttcggaga tgaagcctgg catgaccaat attcctatca
    57841 tgaaagaacc tgtttatgtc gccattccta agaaacatcc tcttgctaaa aaagaacatc
    57901 tgcaattaga agatatccaa cgcaccccca tcttgatgct gtcaaagagc aacgccttcc
    57961 gtcatacact ggatgcagca cttgaagcga aaagtatcga attgacgatc agttcgacga
    58021 cagatgatcc tgcgacatta cgttctattg tcaagcaagg attagctgtt agctttttcc
    58081 caaaagtttc ttggtcttac gataaaaatg atcctttcgt cttgcgggaa atcaaagatt
    58141 tgcccttgac acgaacaatt tatttatctt ctagcttcac agaagatcat cctttgatca
    58201 aaatgattgc aaaaacgatg cgggaatttt atttaggaaa ataaaaaaaa aagagctggg
    58261 acaaaattct tgtcgcagct cttttttcga acaaacggtg ttccagaagt aatttcttag
    58321 aaaataagcc gaaatttccc aaaaatttga gaagcaattt tcgtaaattc cttcttattt
    58381 cttgaagcta aacatttctg tcacaacctc ttctctattg tcctagacat tcattgcgtt
    58441 gatttaaaac ttccatcaat caaatctgct agttcatcta gcttttctat tttacgtaga
    58501 tcaatagaga tccaatgttc tttattcatg tgataggctg gaaaataccc ctcttttcct
    58561 cgcaaaattc cgccaagttc aggatcgacc ttcaaatcaa gaatattgat gatcctatgg
    58621 tcacttgcat catcatagat tttttcttct ggaacagaca tcaggacccc aaaccatttt
    58681 ttgctttttt gatgtctgaa tacgcaataa tcaggaaatt ttttaaaaat atattctgga
    58741 gacaaattgt atgtttcatt gatgtgatcg attagttctt ttttcgttag catctgcttc
    58801 catctctttc ttattgattc gctttattca aagataccac gtaagaatag aaaaatgaat
    58861 acaagtagtt gctaatcaaa tacttcgata tttttctttt ttatcaactc ttaaatgttc
    58921 aatccgcctg tgttggtctt gctaccatct actattaaaa tgtttattat tgtttgccca
    58981 cctatttttt gattttagtg gtatcatttt tcaagttcat gatgaaattc ttatcatcag
    59041 taaatgctcc agaaaaataa acaatgattg aaaaatcagt tgaaacttga ccaccgttta
    59101 ttttctccgt attcttagaa tagaagggag tctgattgat gtattcacta atgaaaaaaa
    59161 tcattaccga aaaagatatt cgacgtcatg tttctttagt agaacaattg ctgaatcata
    59221 caaagataac cgtaaatgaa ttagctgaaa tcatagggac aaccgaacga acgatttttt
    59281 ctgaccttca atctattcgc tctcagctgc ccgaaggctg ggacatcatc agcgatcaag
    59341 caggaatcag tttgcaaaac cagcaaaatt tattaacaaa tgacctttgg gagatttttt
    59401 tcaaacaatc agtcagtgtc gaactgctga aaaatctgct ttttacaaaa aaagttgctg
    59461 tccctgattt tctagccgac catgggcttt cttatgggac attgaaaaga catgtgacta
    59521 aaatcaatca gcgtttagca agttacgact tgcagatcga tttgacgaaa tatacagctt
    59581 gtattcttgg aaaagagcgt gcgattcgta ctttttatca tcgattgctg attcctttca
    59641 ctcataacaa ctattttttc gaggattatt cgatccatga atcccattat tttcaatttt
    59701 taagaaacct cagtcaaact gaactagcgg ttgaaacaga agagattttc ggaacatgtt
    59761 ggttctttat caatacgatc cgtataaaag cgaattgccg attagattct tctatccata
    59821 ccaacagcac actctcctcc ctgtatgact ctgcattgaa aaaattgtat ttaacggaag
    59881 gtatttatct taaagacact gagctttctt ttgcttcttt ctgctttctg gaaagttgga
    59941 attacaacaa taactatgga caagaaaccg ctcgctgcct gcatcattct ccatttttag
    60001 aggtcctcga aacgtttgta gaggaactag cttcagagct gtctcttgat caactaaaaa
    60061 aaacatctct gactgataac ttggctctcc tcatattaaa atatcatgaa tcgccgatat
    60121 tgattgaagc attggaccgt caataccatc gttttttaga aacatatgat cagcaatatg
    60181 cacacttgta tcaacagaaa gagaatttac tgaacgatct aaaaaaagaa atagaaatca
    60241 atgaaccaaa ttactttctc caactcttgt ctctactgat ccaacaaacg attttttcta
    60301 tcaaaccgaa tttgttcaat gtctatttta ttttccaagg agaaccttca tggaaggcgt
    60361 tcttgcagca ggaattaaaa gattacttag gaaaacgtgt ctgcttctta cctatcgaac
    60421 taaccgatct ttccactatt tcttttgaaa aaacagatat cttgatttcc aatttccctc
    60481 tagattctct agatatccct attgtataca tttcttctat tccaacgaaa aatgagctca
    60541 atcgtttgac tgaactcact ttcaaaactt ttttatagta acggctagcg actgtattct
    60601 ctagctgttt tttctttttt gcgaaacagt ttgttcatga tccctttttc tatttctaca
    60661 actaagaata gaagaatcac atttgctcca atcaataact gttgtgtcat cgttaaactt
    60721 actgtaccga tcagctgctg gccgactggt agaaaaataa ccatcccttg caataaagca
    60781 aggataccta atgaaagaaa aagcgctttg ttttgcaaaa gaccttggtt caatgacggc
    60841 ttggacaact ctctgcaatt gatcatgtaa acagcttgag ctaagacgat attttgcaga
    60901 agaatggttt gctgtaagct ttgcccttca aactgcatag ccaataaata ggcaggaacc
    60961 atgatcagta gggagacata gatgatccga aacaaatgat aaccagatag gattccttgc
    61021 tttggatctc ttggtgggcg cttcatgatt tctggatctg gcgactcgaa ccctagtgca
    61081 taagataaag tgattgtcgt taccatattg acccataaaa tctgtactgg ggacaaaggt
    61141 agcgggcgat tcagcataag tgcccaaatc aagatcaatc cttgagctaa tgcagttggc
    61201 aagaagaaat tgatggtttt ttgcaaatta tccaaaatcc gccggccctc ttttacggct
    61261 tttgcaatcg tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact
    61321 tcactacctt tgattcccat agcaattcct acatcggatc ttttcaaagc aggtgcatca
    61381 tttacgccgt ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttaacaatt
    61441 cgcagtttat gttctggtgt cgttcgagca aagacatcga ctattggcac ttgctgaatc
    61501 aactcttcat cagacataag atccaaatcg atcccttcta aaactttggc agtatgtttt
    61561 aagccaattt gttccgcaat cgcttttgct gtgtctttgt gatcccctgt aatcattttc
    61621 actgagatcc ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga
    61681 tcgatcaatc cggcgatccc tgcaaaagtt aattgggtta aagtctcatg cgtgagttcc
    61741 tgtttcgaat ctactttttt ataagcaaat cctaaaacac gctgaccttt tctcgcaaat
    61801 tctgcagctg tctgactcca tgcttcttgt tctgcagaag aaagagttga tttagcaaat
    61861 aatacttctg gtgcaccttt gacgtaaatc accgctcctt tttcctccgc atgcatcgtt
    61921 gccatatatt tgtattcaga actgaacgga atctttgctt ctattttttt caaaggtaga
    61981 ttggctgttt ctgtgtgtcg aatcagcgca acttctgtcg gattgccact taaattttct
    62041 gttttctgtt cttcttttgt ttttaattcc tgacagtttt tcataatgga caacacgcag
    62101 gcttcttctt ttgcagctac atccacaact gtcatttcat tttttgtcaa tgttcctgtc
    62161 ttatctgaac agatcactgt catagaacct aatgtttcta ccgaaggaag tcctttgatg
    62221 atcgcctttt cttttgccat ttcatgtact ccaacggata gaatcatggt caaaacagct
    62281 ggcaatcctt cgggtaccat tgacaccacc aaagcaatcg tagccgaaag aaggatatgc
    62341 caatccatac catatcgaaa tgtcgtaaaa ataactaaga ataggctcaa acaaagaatg
    62401 ccttgaaaaa tttgtttgtt taatcggttg atttttttaa tcagaggcgt cgtttgttgt
    62461 tcgaccgatt gcaatgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca
    62521 acgacaccga ctgcagaacc agattgtaca agcgttcctg aaaaagccat atttttctgg
    62581 tcgccgatcg attcttcttc tgtcaaaacg gcatgacttt tttcagatgc ttctgattct
    62641 cccgtcaaaa tcgattcttc cactaataaa tcatgtgctt cgattaatcg gatatctgct
    62701 ggaacgacat ctccggcacg taagacaatg acatctcctt ttaccagttc tttcgcaggg
    62761 attttttctt gcatcccgtt tgatagtact atcgcttctt gtcccatcag ctgcttcaaa
    62821 ccattcaatg attcttccgc ttttctttct tgccagtaac taacgagtcc attgacgatc
    62881 actacgaata aaataatact tccttcaata tattcactag aagcaaattt caaaaaagag
    62941 gcaatgatca acacgatcat cagcagatca gtaaaatgct ttgctatttt tttccatcgt
    63001 tttgtctgct gccttgcttt tatttcatta tatccttgat cagtcaggcg tttctgtcgt
    63061 tcttcttgcg acaagccttc tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc
    63121 aatctgtacc agttcatcct atcccctcct gtttaattga tactgttatt atacgcaggg
    63181 aagttttttc aggcgtatcg attatttcag gggggtctga aataattcgt tttgcaaaat
    63241 gtatctgggc ctaactgata tttctattgc ttattctttt atatacaaaa tttaagaata
    63301 caaaccagat aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat aacaagctga
    63361 aagcaattga agtaaaaaac accaacaaag caagatcttt atatctttgt gtagtgttcc
    63421 gtttcttttc tttaataaat tgaacatgtg cacacaagat cgaagaccac acaaatagaa
    63481 aacaactcgt tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa
    63541 aatgtagaca aagaccggca aaaaataaca ttcctgtaat taaaataccg tgtaaaggta
    63601 cgcctttctt agaaagcgca caaaaccaat ggggcaaact tttttctttt gctgtttgat
    63661 ataaaatcct gctagtactg tagactgcac tatttgctga ggacaaagaa gcaaccaaaa
    63721 tcattagatt gacaattgct gctccgcctt ggcaatggat cacttgcagc atagtcacta
    63781 aaggactttc ttttaaatct aaacaatgcc atggaacaac aaataaaatc gacaaaatcg
    63841 ttcctatata gaaaaagaga atacgccaag gcagacagtt gattgctttt ttcaaattct
    63901 tttgcggatc acttgtctcg ccaacggtta agccgatcat ttcaattcca gcaaaactga
    63961 ataaaaccat aggaaatgag actaccattc cagcaaagcc aaacggtagt aattcatcga
    64021 tctgtccaaa attttctttg attccgatag actgggtatg aaaatctgag acactcaatc
    64081 ctatacccaa taagataaaa aggaaaatcc ccaagatctt gatgattgag aaaaaagatt
    64141 ccaattcacc aaaccacttg actgcaacaa ggttgactgc cattaagatc attagtgcgc
    64201 tacctccgct tatcaaagct ggtacatgta caaaccagaa atggatatac aatcctattg
    64261 ctgttaattc cgcaagtcca gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa
    64321 atgcccactt tttgcctaaa taatagtcaa cgaaatcact gatagagtta aatgtactat
    64381 ttgctaaaaa taaatcaccc atggcccgca tcaaaccata aatagccaaa ccgcatatca
    64441 aataaacgag aataacagct ggccctgctg atgcaattgc ctttgctgaa cctaaaaata
    64501 atcctgttcc aattgcgcca cctaatgcga ttaactggat atttcgatta gttagagtcc
    64561 gtttcattgc cggtttttcc atcaagttca cctttttctt tttcaagcag catggcgact
    64621 tcctgttccc aagaaggttt ctttattttt tcaatacaga gtggtactac aaacaaaatg
    64681 acactgatgg ctgtgactgc aattagattg atccatgttc cagctttcat cgtcagtccg
    64741 acagaaaatc cgattgccgc aaataaaaca ataacgacag tccaaactcc aagattgctc
    64801 tttttctctt tttttccaat tctaaatggt cgattcaact ttgcatgtgt ataacgcaat
    64861 ctcaaaactg cgatccccat gatgacatag acgatacagt agataacagt agttgaattt
    64921 acgagcatca aaaatgcttg attgacgcca ggaatcagca aataaaaaag tgcaaagatt
    64981 gagataatga cagactgagt caaaataacg gatttggata cgtcaaattg attcgtcttc
    65041 caaaaattga attttggtgg atacgcacct tttctagcac tttctgtcac tgtctttgct
    65101 ggtcctgagg cccaagcaga taattggact gctacaccaa tgaagacaag taaactaaac
    65161 agattcacaa taagatgtgg caaaccaaga atttggcaat aaatcgaaat agattgcgcg
    65221 atattattca attccatttg tccttttggt acgacatttg ctacaactaa agcattcaaa
    65281 atattgacga aaaatataaa aattaacgcg gcaaatactc cgcgaatata cgttttaact
    65341 ggattttcca atcgagtgat atacacagag gacatttcga taccagtaaa aataaacatg
    65401 atcggcgcaa aataaacaaa tgaggatgca gtcgtagtat ctggtattaa tttgtcccat
    65461 gaaaaactgc ctaatgttcc ggttggtacg atcccaactt tgatccacgc agcaaaccct
    65521 aataagagca tcatcgtcaa aggcacgtac aatcctaacc aaacaccgac ttttccgccg
    65581 atttttgcca tatcaaattt caaattcaaa agagtgatga tccaataaac gactaaaatg
    65641 acaactaatg taaatttgct gttcaaacca agcggcacat tatcgataat attaccaaac
    65701 aacggagcga gtgctgaagc gaccatcacc atcccaggaa acatttggac ccataacagc
    65761 catgagacaa cgaaccccca tttttttcct agagcattcg atacccaaag ttctggacca
    65821 cctttttggg gaaacattcc agcaaattca ccagagatca atgagattgg aaaagcgtac
    65881 aataatactg caaccagcat gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga
    65941 atatttcgta cacttgctac aagggcgcag gtcattccga taaaggtcat ggtcgtgatt
    66001 tgctttgatg aaccagtatc tttcatcttt attccttctt tcaatagcga catatgtttt
    66061 tgttttcttt tatttttgtt caagaacaga aatgacaagg tttcatcgat tcagtacaaa
    66121 acccagtatc accgaaacat ccattgaacg atcttagtga actgggtttg atacatgaaa
    66181 cgaacgatcc aacttataga acgttttata aaaaagaata tcttggcgag gaatatatga
    66241 aaaacagggg gaaatacaat tgttttgatg aagcaaataa ttgtctgttc tccatatgtc
    66301 tcttgttatt ctttcacaca aaaatcagtt ctattggatg atttcgttta gtttcttttc
    66361 cattgctttg gcgatttttt ttgtataaaa attgaaaatt cgatcatcat tcaaataagg
    66421 agtcatgata gccgcacgta gcagagtgat tttctgttct tttttccatt cttctttcgt
    66481 tagacccatt ctttcaacaa aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt
    66541 agtatgagaa gtaataaacc gttcaccata aacatcgcca tccatataag aagaccgatc
    66601 gaacattttt tcatttaatt cattgatagc gttcaaatct gtacagcctt gttctttgaa
    66661 tacccaattg accatattaa aatcaggatg attcaatgga tagacttcga ttgtttttcc
    66721 tttaacagta aaagtcagat gatcgagaaa ttctctgaat ctttgagctg cctcgattga
    66781 agccccaatc aattgaccat atccggtcac attcaatggc aatacacgat gtgctgtcca
    66841 gactgcggcg gcggttgctc cagcttttga tccttccaaa atataagctc ctaacatatc
    66901 tggagccttt actgattttt caaacacata tggcgcgaaa taagaaatga tatttctcat
    66961 atttttatgt ttgattacga ttcctccagc agcataaggg acatagccca tcttatgagg
    67021 atctatcgtg acagaatccg cttctgaaat agctctgaac ccttcatata cctctttgtc
    67081 aatcgttacg cagtggtgga aaacatgatg ttcttcaaag aattcagcta aggaagcata
    67141 cggcacaaat tctcctgctt cgtttagaaa cagtgaacgc gcatagccgc catatgcggc
    67201 atcaacatgt aaatagaaat agatcccttc atcttttaac ctttctctta attggacgat
    67261 tttatctaca ctgtctactt gaccttcttc tgtcgttcct acgacagcaa ccacacctag
    67321 aattgggatt ttttgtccag ctaattcacg aattgttttt tccaaagcat taatatccat
    67381 tcgataatcc tcttgaactg ggatagcaac catctgatct aagccgactc cgcaaatatc
    67441 taacgccttc atccaagaat agtgttttgt ttgagggacc agccatttcc ctaatctttg
    67501 gatatttttt ccgctacggg aagaagctgc tttgacctca tccatttctt cgtccgtaaa
    67561 gctttcggtc atttccaaaa tttcttcaac agataaattc aaaagttccc actcagacat
    67621 ttttttcact ttttcaggca gcacttcctt gacagctaaa ggaatcgatt tgatgcaacg
    67681 ggcgtaccat aatccttcaa gattagcaat agaaccatct gctgcaatgt gtccccatcc
    67741 gtctgtcata ttgaataatg aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt
    67801 tgccatagaa gattccaatg caacattatt tgggttccac agcatcgcat agttgtaagc
    67861 taataaagca ggcatcaatg tttcagcatt catttgtccc cagtaacggc ctgctgaatg
    67921 ccatgggatc gatcctgcgc gtaatcgctg actgacttca tccaagacct ctatcatatg
    67981 gtcgcgggtg gctagataat ctggagtgag cttatctcct tcgctaattg caggcatatc
    68041 tgaaggaata taattttctc tccatccaag atgttcatct accatcttgt tcaacaacat
    68101 tttataaact ggtccatttt cagctttgtc gcctataaag acggccttga tatccatatc
    68161 tttcattttt cttccctcca tattttgttg gtcaatgtct tgcagataca tgtgtttatc
    68221 tgtaacacct cttcactata gcgccaaaaa tgagaaattt ttttttataa catcaaatcc
    68281 acgtagattt ttacaaacaa aaataaataa agtcggattt ttctgataat tcaacgcaaa
    68341 aaagcgaaca atttttcaat aaatcagtct tctaaaatag ataacctaaa aaaagcgaac
    68401 aaacaaataa aa
//
LOCUS       DADJYG010000002        65861 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.002, whole genome shotgun sequence.
ACCESSION   DADJYG010000002 DADJYG010000000
VERSION     DADJYG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.002"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            447..1703
                     /locus_tag="JZ382_000061"
     CDS             447..1703
                     /locus_tag="JZ382_000061"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY2426276.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            1994..3871
                     /gene="tdc"
                     /locus_tag="JZ382_000062"
     CDS             1994..3871
                     /gene="tdc"
                     /locus_tag="JZ382_000062"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAY2426277.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4114..5535
                     /locus_tag="JZ382_000063"
     CDS             4114..5535
                     /locus_tag="JZ382_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2426278.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5644..7011
                     /gene="nhaC"
                     /locus_tag="JZ382_000064"
     CDS             5644..7011
                     /gene="nhaC"
                     /locus_tag="JZ382_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAY2426279.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7292..8311)
                     /locus_tag="JZ382_000065"
     CDS             complement(7292..8311)
                     /locus_tag="JZ382_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2426280.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8333..9340)
                     /locus_tag="JZ382_000066"
     CDS             complement(8333..9340)
                     /locus_tag="JZ382_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAY2426281.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9333..9998)
                     /gene="pgmB"
                     /locus_tag="JZ382_000067"
     CDS             complement(9333..9998)
                     /gene="pgmB"
                     /locus_tag="JZ382_000067"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY2426282.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(9991..12285)
                     /locus_tag="JZ382_000068"
     CDS             complement(9991..12285)
                     /locus_tag="JZ382_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY2426283.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12545..14710
                     /locus_tag="JZ382_000069"
     CDS             12545..14710
                     /locus_tag="JZ382_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2426284.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15014..15847
                     /locus_tag="JZ382_000070"
     CDS             15014..15847
                     /locus_tag="JZ382_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY2426285.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(15949..17649)
                     /locus_tag="JZ382_000071"
     CDS             complement(15949..17649)
                     /locus_tag="JZ382_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426286.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            18156..19316
                     /locus_tag="JZ382_000072"
     CDS             18156..19316
                     /locus_tag="JZ382_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAY2426287.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19332..19775
                     /locus_tag="JZ382_000073"
     CDS             19332..19775
                     /locus_tag="JZ382_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426288.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20258..20788)
                     /locus_tag="JZ382_000074"
     CDS             complement(20258..20788)
                     /locus_tag="JZ382_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAY2426289.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            20893..21444
                     /locus_tag="JZ382_000075"
     CDS             20893..21444
                     /locus_tag="JZ382_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY2426290.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21597..22070)
                     /locus_tag="JZ382_000076"
     CDS             complement(21597..22070)
                     /locus_tag="JZ382_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY2426291.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22208..23680
                     /locus_tag="JZ382_000077"
     CDS             22208..23680
                     /locus_tag="JZ382_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY2426292.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23677..24387
                     /locus_tag="JZ382_000078"
     CDS             23677..24387
                     /locus_tag="JZ382_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAY2426293.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            24739..25023
                     /locus_tag="JZ382_000079"
     CDS             24739..25023
                     /locus_tag="JZ382_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAY2426294.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(25304..25723)
                     /locus_tag="JZ382_000080"
     CDS             complement(25304..25723)
                     /locus_tag="JZ382_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAY2426295.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(25725..26309)
                     /locus_tag="JZ382_000081"
     CDS             complement(25725..26309)
                     /locus_tag="JZ382_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY2426296.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(26356..27831)
                     /locus_tag="JZ382_000082"
     CDS             complement(26356..27831)
                     /locus_tag="JZ382_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY2426297.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            28012..28323
                     /locus_tag="JZ382_000083"
     CDS             28012..28323
                     /locus_tag="JZ382_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426298.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(28438..30774)
                     /locus_tag="JZ382_000084"
     CDS             complement(28438..30774)
                     /locus_tag="JZ382_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2426299.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGNI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            30916..31584
                     /locus_tag="JZ382_000085"
     CDS             30916..31584
                     /locus_tag="JZ382_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426300.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            31587..32630
                     /locus_tag="JZ382_000086"
     CDS             31587..32630
                     /locus_tag="JZ382_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2426301.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(32867..33748)
                     /locus_tag="JZ382_000087"
     CDS             complement(32867..33748)
                     /locus_tag="JZ382_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2426302.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            33907..34635
                     /locus_tag="JZ382_000088"
     CDS             33907..34635
                     /locus_tag="JZ382_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY2426303.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            34791..35108
                     /locus_tag="JZ382_000089"
     CDS             34791..35108
                     /locus_tag="JZ382_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY2426304.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            35101..35709
                     /locus_tag="JZ382_000090"
     CDS             35101..35709
                     /locus_tag="JZ382_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAY2426305.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            35725..36555
                     /locus_tag="JZ382_000091"
     CDS             35725..36555
                     /locus_tag="JZ382_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAY2426306.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            36693..37181
                     /locus_tag="JZ382_000092"
     CDS             36693..37181
                     /locus_tag="JZ382_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAY2426307.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            37232..38104
                     /locus_tag="JZ382_000093"
     CDS             37232..38104
                     /locus_tag="JZ382_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAY2426308.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            38280..38642
                     /locus_tag="JZ382_000094"
     CDS             38280..38642
                     /locus_tag="JZ382_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAY2426309.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            38651..39403
                     /locus_tag="JZ382_000095"
     CDS             38651..39403
                     /locus_tag="JZ382_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426310.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            39417..41219
                     /locus_tag="JZ382_000096"
     CDS             39417..41219
                     /locus_tag="JZ382_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426311.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            41496..42536
                     /locus_tag="JZ382_000097"
     CDS             41496..42536
                     /locus_tag="JZ382_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAY2426312.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(42588..44705)
                     /locus_tag="JZ382_000098"
     CDS             complement(42588..44705)
                     /locus_tag="JZ382_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY2426313.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIPETTTRTVKA
                     ATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(45062..46435)
                     /locus_tag="JZ382_000099"
     CDS             complement(45062..46435)
                     /locus_tag="JZ382_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2426314.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(46435..46830)
                     /gene="rbsD"
                     /locus_tag="JZ382_000100"
     CDS             complement(46435..46830)
                     /gene="rbsD"
                     /locus_tag="JZ382_000100"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY2426315.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(46858..47775)
                     /gene="rbsK"
                     /locus_tag="JZ382_000101"
     CDS             complement(46858..47775)
                     /gene="rbsK"
                     /locus_tag="JZ382_000101"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY2426316.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(47883..48896)
                     /locus_tag="JZ382_000102"
     CDS             complement(47883..48896)
                     /locus_tag="JZ382_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2426317.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(48977..49576)
                     /locus_tag="JZ382_000103"
     CDS             complement(48977..49576)
                     /locus_tag="JZ382_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY2426318.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            49762..50463
                     /locus_tag="JZ382_000104"
     CDS             49762..50463
                     /locus_tag="JZ382_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426319.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            50473..53184
                     /locus_tag="JZ382_000105"
     CDS             50473..53184
                     /locus_tag="JZ382_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAY2426320.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            53581..55209
                     /gene="treC"
                     /locus_tag="JZ382_000106"
     CDS             53581..55209
                     /gene="treC"
                     /locus_tag="JZ382_000106"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY2426321.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(55269..56450)
                     /locus_tag="JZ382_000107"
     CDS             complement(55269..56450)
                     /locus_tag="JZ382_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY2426322.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKCLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            56702..58225
                     /locus_tag="JZ382_000108"
     CDS             56702..58225
                     /locus_tag="JZ382_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAY2426323.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(58294..59022)
                     /locus_tag="JZ382_000109"
     CDS             complement(58294..59022)
                     /locus_tag="JZ382_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426324.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(59035..60315)
                     /locus_tag="JZ382_000110"
     CDS             complement(59035..60315)
                     /locus_tag="JZ382_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2426325.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(60456..61058)
                     /locus_tag="JZ382_000111"
     CDS             complement(60456..61058)
                     /locus_tag="JZ382_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAY2426326.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            61303..61509
                     /locus_tag="JZ382_000112"
     CDS             61303..61509
                     /locus_tag="JZ382_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426327.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            61821..62540
                     /locus_tag="JZ382_000113"
     CDS             61821..62540
                     /locus_tag="JZ382_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAY2426328.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(62621..63304)
                     /locus_tag="JZ382_000114"
     CDS             complement(62621..63304)
                     /locus_tag="JZ382_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAY2426329.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            63522..65708
                     /locus_tag="JZ382_000115"
     CDS             63522..65708
                     /locus_tag="JZ382_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAY2426330.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        21053 a        11736 c        13381 g        19691 t
ORIGIN      
        1 aataattgaa aataaaatgt tcggaattag agcggattca aaaaagaaaa aatgaggaat
       61 ttaaaaaaaa tatccttttt ctgttgacaa agaaaaagtt gcgctctatg atagacacat
      121 attaataata acaagacgaa gatcgaaacg aagtagttgt taaagccaat gtttcagaaa
      181 gctggtggtc gctgcaaacc agtacatgct ttaaagcgaa ttacatttca ggagtctttg
      241 gcagtgattg ccacgcgaca gcgttaaatg ttcgagaggt atgttggata tctagacata
      301 caaattgggt ggtaccgcga gatttcgtcc cttggcttat atgccaaggg acttttttta
      361 tttcctatcc tatcctgaag aaataattaa ttacatatgc tagcaggttc ctgctttcat
      421 aaaaaaacta cacggaggtc agtaaaatga atattattga tgaattaact tggcgcgacg
      481 ccattaatca gcaaacaaac gaagaaagac ttcgcgagct cgttgaagaa aagagcattt
      541 cactttattg cggcgttgat ccaactggtg attcgatgca tatcggacat ttgattccat
      601 ttatgatgat gaaaagattc caattggcag gccatcatcc atatattctg attggcggcg
      661 gaacaggaac gatcggcgac ccaagcggcc gcaaaacaga acgtgtgttg caaacgatgg
      721 aacaagtgca acataacgtg gacgcacttt ccaatcaaat gagaaaattg tttggtaaag
      781 atgcaaatat cacatttgtg aacaactacg actggttgtc aaaaatctct ttacttgaat
      841 ttttgagaga ctacggtaaa aacttcaaca tcaacacgat gttagcaaaa gatatcgttg
      901 ccagccgttt ggaagtagga atctcattta cagagtttac ttaccaaatc ctgcaatcta
      961 tcgacttttt gcatctgcat aaaacatacg atgtgcaatt gcaaatcggt ggagcagacc
     1021 aatggggaaa tatcacagct ggattagacc taatccgaaa attagaagga ccagaagcag
     1081 aagcgttcgg tttaacgatc ccattgatgc tgaaggctga cggaacgaaa ttcggaaaaa
     1141 ctgcaggcgg tgccgtttgg cttgatccga agaaaacttc accatttgag ttctaccaat
     1201 tctggttgaa tcaagatgat cgcgatgtag tgaaatactt gaaattcttt actttcttgt
     1261 ctcaagaaga aatcgaagac ttagcgaaaa aagtagagac agaacctgag aaacgtgaag
     1321 cacaacgccg tttggcagaa gaagttacaa gatttgtgca tagtgaagaa gacttgaaag
     1381 aagcgcaaaa aataacgcaa gcattgttct ctggaaatat taaagaacta aatgcggaag
     1441 aaatcgcgca aggattcggt aaaatgccga acgttgaaat ttcaagcaca cctgaaaata
     1501 tcgtggaact gcttgtttcc acaaaaattg aaccatctaa acgtcaagca cgtgaagatg
     1561 tttcaaacgg agctataagt attaacggtg accgcgttac cgatttaaat tttgtcataa
     1621 atccatccga tgaattcgat ggtaagtttg tggttattcg aaaaggtaag aaaaattact
     1681 ttttggccaa agtaattgat tagaatttag taagttttta ccttaaaaaa gggaaatact
     1741 attacaacat tccaattttg aaacccatcc taaaatagaa cgctggttgt cgttaataca
     1801 atctttgcga gggagtttac atttgtaaat tccctcgttt tttttaatta tttcaaaagt
     1861 gtgaacaatt cggataattg atacatatta aaatatgatt aaaaatcagg ccaaaactta
     1921 gtttacagaa tagaagaaca ggtgtaatgt agggctcgac aaaatttaaa tattttttta
     1981 ggagggtttc actatgagtg aatcattgtc gaaagatcta aacctaaatg ccctattcat
     2041 tggggacaaa gctgagaacg gccaaatcta taaagctttg ttgaatgaat tggtggatga
     2101 acatttaggc tggcgtcaaa actacatgcc tcaagacatg cctattatta cgccagaaga
     2161 aaaaagcagt gctagttttg aacacactgt gaataaaaca aaggatgttc tatcagaaat
     2221 ttcagcgcgg atgcgtaccc attccgttcc ttggcataat gccggacgtt actggggtca
     2281 tatgaactct gaaacattga tgccatcttt actagcgtac aattttgcta tgctatggaa
     2341 tggtaataac gttgcctatg aatcttctcc tgcaacaagc caaatggaag aagaagtagg
     2401 aatggaattt gcaaaattaa tgagctataa agatggctgg ggccacatcg ttgctgatgg
     2461 ttctttagca aacttagaag gactttggta tgcccgcaac atcaaatcat tgccgcttgc
     2521 aatgaaagaa gtgacacctg aactagttgc tggaaaaagc gattgggaat taatgaactt
     2581 gtcaacagaa gaaatcatga atctgttaga cagcgtccca gaaaaaatcg acgaaatcaa
     2641 agctcactca gcacgtagcg gaaaacattt agaaaaatta ggaaaatggt tggttccaca
     2701 aactaaacac tattcatggc taaaagctgc cgacatcatc ggtatcggtt tagaccaagt
     2761 gattccagtg ccagttgacc acaactatcg catggacatc aacgaacttg aaaaaatcgt
     2821 tcgtggtcta gcagctgaaa aaacaccaat cctaggtgta gtcggtgttg taggatcaac
     2881 agaagaagga gcaatcgacg gtatcgataa aatcgttgca ttgcgtcgcg tattagaaaa
     2941 agacggtatt tacttctact tacacgtaga tgctgcttat ggcggatacg gccgtgcgat
     3001 tttcttagac gaagacaaca acttcattcc atttgaagat ttaaaagatg ttcattacaa
     3061 atacaatgtc tttacagaaa acaaagatta tatcttagaa gaagtacaca gtgcatataa
     3121 agcaatcgaa gaagcagaat ctgtaacgat tgacccacat aaaatgggct atgttccata
     3181 ttctgccggt ggtatcgtca tcaaagatat ccgcatgaga gacgttatct cttactttgc
     3241 gacatatgta tttgaaaaag gcgcagatat cccagcatta cttggggctt atatcttgga
     3301 aggttcaaaa gcaggggcaa cagcagccag cgtttgggca gcacaccatg tcttaccttt
     3361 gaatgtcaca ggatatggta aattaatggg tgcatcaatc gaaggtgcac atcgtttcta
     3421 caacttcttg aatgacttgt catttaaagt aggagacaaa gaaatcgaag ttcatccatt
     3481 aacttatcca gatttcaata tggtagacta tgtattcaaa gaaaaaggca acgatgactt
     3541 agttgcaatg aataaattga accatgacgt atatgattat tcttcatacg tgaaaggaag
     3601 catttacggt aatgagttct taacttcaca tactgacttt gctatcccag actatggcaa
     3661 cagcccatta caatttgtga accaactagg attctcagat gaagaatgga accgtgccgg
     3721 aaaagtaact gtattgcgtg ccagcgtaat gacaccatac atgaacaaag aagaacactt
     3781 tgaagaatat gctgaaaaaa tcaaagcagc tcttcaagaa aaattggaaa aaatctacgc
     3841 agaccaatta ttggcaagcg aagcaaaata agcaaggtaa tcatgttaga aaacagcttg
     3901 aatactaact gtttctgacg catgtttgga tctgaagagc aaggggagac ctaagctctt
     3961 cgatcccttt gttttgaggt tgtctttgaa gaaacccttc aagcaataaa ctcaaagaat
     4021 cccctcaaat attcgctgaa ttgtttcgtt tattgctgaa actacttcaa agacaccgtt
     4081 attttatcca aaaaatcaag gagagtgtca acggtgagta aatctgaagt aagtaaaatg
     4141 ggtcttggga cctttatcgg tctgacaatg gcactgtgtg caacggttcg gagtattcct
     4201 acgttagccg cagtcggttg gacattgatt ttttattcaa tttttgcagt tttgtttttt
     4261 gccggtccga tatcaatggt ttccggtgag ctttccacga tgcttcctca agaaggcggt
     4321 cctcagttat gggtaaaaac agcactgggc agcaaatggg gatttgtcgt tgcatggcta
     4381 ttatgggtgc aaatgttccc aggtatggta atggtcgctt ctactttagg accattgtta
     4441 ggaaacacat ttggtaatgt agaacttggc aataatcact ggttcgtttt aggttgtatt
     4501 ttagttatct actggatcat cacgatcttg aacttgaaat tcgatatggc aaaagtcggc
     4561 gggaacatcg gtgtatggct aggggtatat atcccagtag tcatcatgtt cgttttaggt
     4621 gttttggcag ccttcaaagt cggactagtc tcaaacggct atctaggtga cttttcatgg
     4681 tcaaaagctt tccctgactt ggaacatatt gattcattga aatacttagc tggtatcaca
     4741 tttatttttg ttgggatcga aatgagttct gtttacatgc ctcgtttgaa agacgcaaca
     4801 aaaaattata caaaaggcgt atttatcgct ttgatcggat tagttttatt gaatgtcatc
     4861 aatgcgatgc ttgtagcaaa tgtcgtacca gatggaaaaa tggaattagc aaatatcacg
     4921 cagcctatct tgattgactg tcaaatccta ggcttaccag aagtgatcgg aaatatcttc
     4981 agcttcatgg tatttattgg tgtgctttta caattatcag cttgggttac tggaccatca
     5041 aaaacaatca tccaagtagc tcgtgaagga ttccttccac caaaattcgg attccataaa
     5101 gaaaacaaat acggcgtatc tagaaatgtc gttctaacac aatcaatcgt tatttcatta
     5161 tttgctttgc tttatggtgt aatggatgac gtaagtgctg tattcttgac acttacaaat
     5221 gctacaactg ttatttattg tatcgtgtac attttgattg ctgtttcatt gcttaaaatg
     5281 cgtaagaaac atccagaatt cgaacgtcct taccgaattg gtaaaaacgg aaacggatta
     5341 gcttgggtag tcagctgcat gctgatcttc tctatcatcg ttgtcgtatt tgctacatta
     5401 ggtacagcga cattgagtga tgcattatta gtagcagcta tcacagtggt catgttcgtt
     5461 attccattga tcatcaatca cttcaagaaa gattcttggg ggattgaggt agaaaaaagt
     5521 ttagaagaaa aataaaaaaa taagtctttc agccgacgtt ttgtcgctga tttagtagga
     5581 ggtcctgcca agtgaatagt tttttcatgg cagggccttt tcatttgaaa taggagatcg
     5641 acgatgaata aaaatatttc tttaaaagaa agtatcgctg tattgatcgt gttgttggca
     5701 ctcctaggag tactgatcat cggattccag ctctcgccac atatcccgat ccttgcagct
     5761 ttcatgtgtt tgttgtttta tggcagattt aaaaagttca catgggatga gatccatgac
     5821 gggatcatca aaggaatcac tccagggatc attccaatcc tgatttttct tttgattggt
     5881 gtcttagttg cttcatggat tttatctgga accatcccta cgattatggt ttatggattt
     5941 aaacttgttt ctgtgcgttt ctttttaccg acagcatttc ttgtttgtgc aattgtcgga
     6001 gtgactgtcg gcagttcatt tacaacgatt tcgacgattg gaattgcttt tttggggatc
     6061 ggtcatttac ttggattcaa tgatgcaatg actacaggcg cagtcgtttc cggtgccttt
     6121 ttagggaata actgctcgcc tttatcagat acgaccaact tggcagcggg tattggagga
     6181 gttaatctat tcgaacatat cgctggtatg cgttatacgg atttcccagc attcgtcctt
     6241 tcgttgattt tttatacgat attaggacat aacagtcatt cagccgatct agctagtatc
     6301 aatgaaatga tccaaaacat gaaagcgagc ttttggatct ctccttggac attgattcca
     6361 ctagttattt tatttggggg ggcaatcaaa aaaattccgg ctatccctac attactagcg
     6421 ggatcgacag ctgctctcgt tctttctttt atccatgaga aaagtttgac ggtcggaaaa
     6481 gcagcagaca tcttgatgaa tggttatgtc gcaaatacac ctgatccaaa gctagatgaa
     6541 ctgctttctc gaggcgggat catgagtatg ttaggttctg ctagtttgat tttacttgca
     6601 ttggcattag gtgggttact gatcaaatat ctgatcgttg ataccatcgt tcaagaatta
     6661 aaagaaaaga tggaccgacc ttctcgttta gtcgggttta ctgccttgag ctgtatcgga
     6721 atcaatctga tcgttggaga acagtatctg tccatcattc ttccaggaga aacattcaag
     6781 cgttcttttg atgacgttgg cttatcaaaa acctatttga cacgaacact agcggattca
     6841 ggtgcgaccg tcaattctct cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg
     6901 atgaaagtta gcgcgttgca ttacttgcca ttcgcctttt tctcgattct tgcacctgtc
     6961 tttacaatat taggcggttt tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa
     7021 aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaactc aagaaaattg
     7081 ctttttaaat ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt
     7141 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttttt
     7201 acagataaac aatgtgttag aagtagtatc ttcttttaca taaaaagctg accataggat
     7261 aaatggtcag cgtatttagt gagtcagcta tttagtatgg gttttctaaa ggaacgactg
     7321 tctctctttt gatcaagcga tgagggatga ccaaacgtac gccactgctc aattcttcat
     7381 ggatcaattc gcgcagcttt tgcatagcga cacgtcctaa ttcggagata tcgatatcga
     7441 tacttgtaag gtaagggtgt gttaatgttg agaagatcga gttgttgaag ctaatgacgg
     7501 agagtgtctc tggaatgaca tagccataca tttgcgccaa ctgcatcgtg cgaagggcga
     7561 agatatcgtc aatgaccacc aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg
     7621 acagataatc ctccggatgc tctagtacta cactggataa cgcaggaaga ccagccatca
     7681 tcattgcttt ttgataaccg aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg
     7741 tcacaaacag gatattctta tgtccattct ggatcaaaaa ttctgttgct tgtttaccta
     7801 gaagctggtt atcattatct acataaacga tttcgttctc atgatgataa ggctgtccga
     7861 tcaaagtgaa agggatgtgg ttttggtata aataatcaat gacagtatct tgactatctg
     7921 aatacactag aataaaacca tctacttgtt tttgtaaatg catacgctgg acgttctcta
     7981 acagcaattc aaaattttta gcagtagcga ttgctgtagt catatcataa ttccgcgctt
     8041 cttcgttgat tgcttccatg atctcaagat aaaatgggtt accgatccgc tcttttgaat
     8101 caagaggggg taagattacg ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag
     8161 tcatgttagg aacataacct agttcatcca taatttttcg gactttcttc tttgttgtct
     8221 cggaaatact aggatgatcg ttgatgacac gagagacggt cgaggtggca acaccggctt
     8281 ttttcgccac atcttttacg gtaatagcca tgataaaaac acctttctct tgttattgtg
     8341 cagagaaccg ataaatcgtc tgactgatcc gttcttgacc aggatggagg ttgatcgatc
     8401 caaatttggg gaaatgaacg atatcaggga tttcttgcgg ttcgattgct aatccgtagt
     8461 ttgaatgcat ctgttttcca ttgacagaga aatcagcttc gaatccagtc gtagaaaata
     8521 ataccatact tttattagaa gttgctatag tcatttggcg accagaatgc ttctcgaata
     8581 attgtaagga agttttggaa agcctaggat gatgcaaaag aaatacatca tctagtccat
     8641 ctggataaca tgcaaggatc tcttgcagtg ttggggattt tctaaagtcg attggcagtt
     8701 tttctttttt tgtccaattt ccagtaggca gtttttcttg atctaattca agcacaccat
     8761 ctaaaaagac cgttaactga tgttcataga tgtctcttgt tccatttcct gaaaggttga
     8821 agtaggcatg gttggtcgga ttgcagatgg tttcaacaag gctactagca gaggttgtca
     8881 tctctaattc gttttttgtt aaacggtaag tgatcgtagc agtgattggt cctggatagg
     8941 tagaaaattc atcttttaag taaaagacca cacctattga tgatggagtt ttgaaaactt
     9001 ctacttccca aaattggaaa gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc
     9061 agtttttctc caatctatgc tcaccccaag aagcatcccg gatccttccg gctacaggtc
     9121 cgacagttgc tccaaaaaag cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca
     9181 ataaaacatt ttctgatttt ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac
     9241 cgtaattgag aagaacgact tctaagtagt cgttcttcaa tgtgattttc tttatttcag
     9301 tctctggaac tggtacgacg gagatctctg gattatccat ggtcatgcca cttttctttc
     9361 aagtaatcga tcgtaagaaa agtcgtatca gggacaagcg ggatctcctc tccaaggtct
     9421 tcagcacgtc ccacgccaat tggttgagcg ccacttgaga ggatggcttg gataccggct
     9481 tgcgcgtctt cgatcccgat acattcttct gcttttagtc cgacagcttt tgctgcaagg
     9541 aggaagatat ctggcgcagg ttttccgttt gttgcttctg ccgggtcggc aatagcatca
     9601 aagtaaggtg taagacccat tttttcaagt aggaatggcc cgtttttact tgcagaagca
     9661 agagcaatct tgattttttc ttcttttaaa gaatccagta aaggcacgac cccggggaag
     9721 acatcttttg gttcgatcgt ctggatcatc tctaaataat aatcgttttt tcttttcgct
     9781 aattctgcaa attcttcctt tgaaaaagag tgttcttttc cgccataagc taaaagcaaa
     9841 gagagtgagt cttctcggct gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta
     9901 atgccgattt cctgaccaag ctttcgccaa gcacgataat ggaattcagc agtatcggta
     9961 atgacaccgt ctaaatcaaa caatacccct ttaaacattt gcttcctcct ttcttagcgt
    10021 tatttctaga cgatccgcta aagaataaga ctgttcatag acgtaaattt ctagaggaga
    10081 accttctaat aactcgaaag taacttgttc atctgtgact gagatgaaca ataaacgtcc
    10141 gcgataattg atatggaaag aataagcttt ccaagttttt ggcagaaatg gtgcgaatct
    10201 caactgatca tgggcagttt tcatttgagc aaatccttgg acaatcgaca accagctacc
    10261 agtcatggaa gtgatgtgca agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa
    10321 gcgagctgtt cgctggtaca tttctacagc tttgctttcc attcctaatt ctgctgctaa
    10381 aatcgaatgg atacttggcg aaagggaaga ctcatggaca gtcattggtt catagaaatc
    10441 aaagtttttt tgcttttctt cttttgtaaa agtatcattg aagaaataaa tcccctgtag
    10501 tacatctgcc tgcttgatga agcaagatct aaggattttg tcccaagacc aatgctgatt
    10561 cagaggaaga tcgcttgtgg gaatatcgga tacaggcatg agatctttat ctaagaatgt
    10621 gtcatgctgg acaaagattc ctaattcttc gtcaactggg aagtacatgt tctctgcgat
    10681 atctttccat ttggctagtt cttcttcgct gatagtgata gcagcttctt tttgataagt
    10741 taaataattt tctcgcgtgt aacgcaagac ccaagctgcg atcgtatttg tataccaatt
    10801 gttattgata ttgttctcat attcgtttgg gccggtcaca ccgtggatca tgtatttatt
    10861 tgctcgtttt gaaaaatgga cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc
    10921 cagtccttca tttttcaaat attccgtatc gccggtgtaa ttcgtataat tgtagattgc
    10981 ataggcaatc gcgccatttc ggtgaatctc ctcgaatgtg atttcccact cgttatggca
    11041 ttcgacacca gtgaaggtga ccataggata aagcgcgcct tttaatcctt gttgctgagc
    11101 gttgtggatt gcttgcggca gttgattatg ccgatatttc agcagattct tagttacttc
    11161 aggtttggct aaagcaagat aaagagggac agcatacgct tctgtatccc agtaagtggc
    11221 tccgccgtat ttttctcctg tgaatccttt ggggccgata ttcaatcgtt catcttcgcc
    11281 gtagtaagta gagaatagtt ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc
    11341 tccttcgatg accacatcgg caagcttcca tcgttctgcc caagccgctg tttgctcttc
    11401 ttttgcttgg atataattgt ttgtctggtt cgttaattct gagacagctt tttctgcttg
    11461 ctgcgcttct ggaatatctc tactagtaag aacgataatt tctttttcca ctgaccatgt
    11521 ctcaccttga gataattctg cagacaagca accatgtaat gctaaagtat cttcgtgata
    11581 gtcagggatg actgattttc cattaacgaa atggcgcatt gctgcagtaa cagtaaattg
    11641 ttcaatccca aagttgtttg gaatagtttt agtcgtcaaa tagccgattt cttctatagt
    11701 tccacgatca atctcattcc aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt
    11761 tccatccaat tttgatacga gtttgactgt tccgctacct tcaagcattt ctatagttaa
    11821 atagatataa gctgattctt ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac
    11881 tttattagta gaagtagtga ttgtgaatgt acgagataat acaccatttt tcatatctaa
    11941 ttcccagtaa aagtcttctg gttccaaaga agccaagtcg atcagctgat cgttgatcga
    12001 aatatctatc gcgataaagt tcaaggcgtt gatcactttt ccaaagtatt ctggataacc
    12061 atttttccac cagccgacac gtgtcttatc gggataccag acacccgcca aataagtacc
    12121 ttgatggtgg tcccctgagt aagtttcttc aaagttcccg cgcatcccca tataaccgtt
    12181 tccaatactt gttaaagatt cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct
    12241 gattttccat ggatcaatag caaatagtcg tttgatctgt ttcatttttc tcctcctaat
    12301 gagctgtttt atacgttcat aataaacgca accgattgca caagtcaata gacaaaagaa
    12361 aatatttcat agaaaaaagc ttaaaaaaat aaatttttag gagaaaatta tagaagggaa
    12421 cacacgtatt ataggtttta tagaatggta tagacagaaa aataaaaaat aaagcattta
    12481 caaaaaagaa aacgcacgct ataatacgtt taacgaaacc gattgcgtaa aggagctaaa
    12541 cggaatgaaa aagatattta gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt
    12601 tgtggtagca gttatgccgg cagctgggtt gatgatcagt atagggaaat caattccctt
    12661 gatcaatcct gattggacgc cgcttgtcac aacgggcgga gtaattgaaa atattggttg
    12721 ggcaattatt ggtaatcttc atattttatt tgctttagca ataggaggaa gttgggcaaa
    12781 agaaagagca ggcggagcat ttgcagctgg gatctcattt attttaatca atcggatcac
    12841 tggggctatc tttggtgtca caagtgacat gcttgcgaat gaagatgcat ttacccacac
    12901 tctgttcggt acgaagatca tggttaaagg attttttaca agtgtattgg aagcaccagc
    12961 tttgaatatg ggagtattcg tagggatcat cgctggattt gtcggggcaa tggcttataa
    13021 taagtactat aattatagaa aattgccgga tgctttgtca tttttcaatg gaaagcgctt
    13081 tgttccgttt gttgttatct tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg
    13141 gccaaacgta caggctggaa tcaataattt tggtttatgg attgcagaat cacaagaaag
    13201 cgcaccgatt ctagcaccgt ttttatttgg aacattagaa cgtttactat tgccatttgg
    13261 attgcatcat atgctgacga ttcctatcaa ctatacacag cttggcggat cgtatgagat
    13321 tttatcaggc gcacaggcgg ggacacaagt atttgggcaa gatccgttat ggttggcttg
    13381 ggccactgat ttagtgaatc ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt
    13441 gcataactgg acgccggctc gtttcaaagt aggacaaatg attggttctt caggtatctt
    13501 gatgggaatg gcttttgcga tgtatcgtaa tgtcgatcct gataagaaag caagatataa
    13561 atcaatgtat ttttcagcag cgttggctgt ctttctaaca ggtgtaacag aaccattgga
    13621 gttcatgttt atgtttgctg ctgtgccatt atatgtcatt tattcagtga tccaaggggc
    13681 tgcatttgcg atggcagata tcttgccatt gcgggtccat tcatttggga atatcgaatt
    13741 gctgactcgg acaccgttag ctatcaaagc gggtctaggt ggagatctta tcaacttcgt
    13801 gttgtgtgtg atcgcttttg gtgttgttac gtatttctta gcaaatttct tgatcaaaaa
    13861 attcaacttt gctacaccgg gacgtaacgg aaattacgac aatgacagtg aagaaaccgt
    13921 tgtatctaac agcggaacag gaacagcaga tcagcaagtg gtccagatca ttcatttgct
    13981 gggaggaaaa gaaaatatca aagatgtcga tgcttgtatg actcggttac gcgtaagtgt
    14041 aaatgatcga gaaaaagtag ggactgaaga agcctggaaa cgagcagggg cgatgggatt
    14101 gatcgtcaaa gacaatggag tccaagcagt ttatggacca aaagcagatg tcttgaaatc
    14161 agatatcgaa gatctgctgc aatcaggagc agagattcca gagccagaaa tggtttctac
    14221 aggaaatgga caagcaggta gtaagcaata cttaggtata gaacaagaac tagtttccgc
    14281 agcttctgga gaagtgattc ctttatctga agtaaaagat ccggtattct cacaaaaaat
    14341 gatgggtgac ggctttgctg ttattccaac atcaagagaa gttgttgcgc cgatcgctgg
    14401 aaaagtgaca agtattttcc ctagcaaaca tgcaattggg ctggaaacga aagatggtat
    14461 cgaagttttg atccatatgg gaatcgatac agtccaaatg aatcagccag cttttgagat
    14521 tgctgtcaaa gaaggccaag aagtaggaac aggaacgaaa ttagctgaga tggatcttga
    14581 tgtgatccaa aacgaagaaa aagatccaac gatcatgatc gtatttacaa acgataaagt
    14641 agaagaagta gtgatcaagc aattaggtac agtgacagct ggtaaagtga tcggctccat
    14701 taaaatgtaa ggagaggttg tgaaaaagct gtcctgcatc aagtcataag aacagccaaa
    14761 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgagaag cagcagtttg
    14821 aatactgttt agtggagagg tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc
    14881 aaacaaaaaa tagctcctgg tccattgagc ttattactga ggagttagca ggagaacact
    14941 atttgctgga gaggttgtga caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac
    15001 aaggagaaaa ccaatgaaat tcatgacact caacacgcat agctggcttg agccagaacc
    15061 agagaaaaaa ttgcaagaat tagcagataa gatcctgtta gaagattatg aagtgattgc
    15121 tttacaagaa atcaaccaat tactggaatc tgaagaagta gaacctgcga agctaatgaa
    15181 gttctgtccg gtaaagaatc aagtacctat ccgtaaagat aattttgcct accgtttagt
    15241 tcagctttta aaggaacacg gaaaagagta tttttggagt tgggaaatga gccacatcgg
    15301 ttatgataaa tatgaagaag gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt
    15361 ccttaccgtc tcccaatttc aaaagaaaga agattatcag acacgcaaga tcttgattgg
    15421 gaaaacaaag atagatgatc aggacatttt tgtctcgagc tgccactttt cttggtggac
    15481 agataaaaaa agtggctttt attttgagtg gaaaaacttg gaaaactact ttttggaaac
    15541 tcgcgtacca ctttttttcc taggtgattt caataatcca gtagactctc aaggttatta
    15601 tactgtaaga gaaagttgcc ttcttcttca agattcgtat gttgtcgcga atgaaaaagg
    15661 aaaagcagct actgtggaaa agaaaataga tggttgggaa caaaatacag aaaaattgcg
    15721 tatagatttt attttcgtcc ctgaaggaat gcaggttaaa aaatatcaac ggatttttga
    15781 tgggatcgat agtccaatca tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa
    15841 cgaataaaaa atataacata taacataaaa aaactgatta aaataaaact ctccaaacga
    15901 ttataccgtt tggagagttt tttgatcgga tgtatttatc ccttttgcct aagcacgtaa
    15961 aagcttgtat ctatgataaa taaataaaca tagcaaggta aaccagccaa tcaaagtaag
    16021 agaaagaatg aaatagaaat agattggcgt ttgataatga agcgtgagct cgttgattcc
    16081 tttttgctgc ttcacgattg gggtcccgat atctgttact tcataatcag tcaatgtttt
    16141 tccattttgc tgtagcacag tccgatcgta gacgataaca gggatatgaa cagttttgtc
    16201 cccagtgttt ttccacttga ttgtcaaaag accgcctgga tgtgttttta taaaagttgt
    16261 ttcgttttct agtatttctt cgttataccg gtcgtattta ttgtcttttg tctccttata
    16321 gattggcaga tagtcaggtg tggttttttg gaaaacgaac aagctggtag acatatcttt
    16381 gctgaagaat gttttccttg cttcttccgg cgtatcgaat agataagtat gtcgactgac
    16441 aaatgtttca ttttcccact tttccagatg ctggtataag ttctggatcg tctgtgctga
    16501 aaaaataaga atgccaaata tacttaaaag acggaaccac ttttgttcag accactggtt
    16561 cagtgaaagg gcaaggtaaa gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg
    16621 aaactggatc aaactaataa aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa
    16681 gctagtagaa agaaaaaaga aaacaagact tgtgtacaac acgatttttt ggaataaggt
    16741 gttcttttgt tgaaagaaaa taaaaagcaa aaaacctgat aatatcaata taggaaaggc
    16801 cttagggtaa tggagccaat aactgccatc cagatctatc gccttttgac tcatttctct
    16861 gttaacaaag ggaggaagaa tcttattccc actgtaaaca atagctaatc ctgcccaaat
    16921 atttgctgtc agaacaaaat aaagcaagac cgacttaatc agttgaagca gtgcttttcc
    16981 tttttcagtc tggttaaaaa agtaataaag ataaaaaggg atatacatca aagctaacat
    17041 gatcgaagac agtaaatgga tctgtgtcat aagtcccact gagacagcga ctttgataac
    17101 agggaacttt ttatgaagga cgaaatcagc aatagggatc atacataaag gataaaaaca
    17161 agtcccccag ctagtgaatc cttggttgat agaccagtat tgaatagaat acgtactcat
    17221 gaataaaacg gctactggta cacttaccca atttcgaaca ttgacatatc tcagtaaaat
    17281 cagcatagaa aatcctgaaa taagataaag aatgaaattt gaaagaatct gataatgaaa
    17341 ccagctacct gcaagcaaca cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac
    17401 ggcattcacg atcctgccac tttgctggaa tccataaagg gaaggaaaat aatggaaatc
    17461 cccatattta atttgttgcg cggcatcata gaaccgattg taatggaatg gggcatcagc
    17521 acccaaaatt agtttatgtg tgaataaatg aggcatgaga aatgccaaag caatgaaaaa
    17581 aattgctaaa aatgggccat attttttcaa ttgtcgaact cctttgcaaa ctttagataa
    17641 attcatcata tatcctatgc gagaggtagg caagaaatta gaaaattcat gaaaatattt
    17701 atgaatttta caagaaaaac ataagtgaaa gttctaagga gagaggatgc gacataagtt
    17761 ttgttgcatc tccttgtttt gaaacagaat ttttcttaat actcgaaaca aagtagcttt
    17821 ttcatgacct ctcggtaaac ggtgttcaat cagctgcttc tcggtaataa gctcactagc
    17881 ctagaaaata tgagaagcta ttttctgact agcgctctta ttactcaaag cataacgcag
    17941 ttttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    18001 cccaacaaaa aatatgagta gctatttttt tgggctgtac ttattacttg atgcaggaca
    18061 gctttttcac aacctcatcg gctttcattt tctatttttt tcctgaaccc atataataaa
    18121 agaaagaaca aaaaaacttg caagggagga cacatgtgaa gcagagtaag caaaaacagt
    18181 ctcgtcgact tttgtttcgt ttgtttttat taatatgtat cgtcgtaggg ttactgatcg
    18241 ctttatatcc attttatgtg gattctttaa atagttttat tgatcaaaaa cgtatcgaac
    18301 aagtacaaaa acggaccgca gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa
    18361 accaacgatt aactgaccaa ggattcaatc ctggtactga tcctttcaat gaaaagaaca
    18421 gaaatgaaag tacaacatcc agccaattag aggaatggct gatcggaagt atcaatatac
    18481 ctaagatcca attaaatatt tcactttatg atcggctcaa tggtaggatc cttgaaaatg
    18541 gagcaggcgt cttgcaagga acctctttcc cgcttggtgg aaattcgacg cattcagtga
    18601 tctctgctca cagcggttta cctaatcgac ggttatttac cgaattagat cgtttagaaa
    18661 acggagacac gtttattttg actgttttgg gggagaaatt ggcttatcaa gttaaaaata
    18721 tacaagtggt actgccagat gacacgtctg ttctaacgat ttcagaagga gaagacttag
    18781 ttaccttgct tacatgtacg ccgtatatga tcaacacgca tcgtttgcta gtgacaggac
    18841 atcggattcc ttataacgaa tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa
    18901 ccatgcggca atggtttatt cttgcaggaa cgataatagc tgtagtcata ctgttagtgt
    18961 ttatcggacg actaatctat cagtatagat taagtaaaaa agtgcttgat ttttcattta
    19021 ttattagtga ttcgtcaggg aaaccggtaa atggtagttc attcattttg aaacataaga
    19081 aaaaaacact tactagaaat ggtgtgccgt tttcagtaca aagcgaccat catgggaaag
    19141 tcaagctgga tcaattgcct ggtggaactt atcgaatcgt ttcagctgat gatcctaaag
    19201 ttgctgtttc ttttggtatt agaaaagtaa aacaagagaa gatatacttt tttgaggggc
    19261 gaaaacttgt aaaagagctt caaaaaaatg gtttttattt taaaatcaat gaataagtaa
    19321 ggagagaaaa aatgatccgc acaacaacaa aattgtctgc caaagaactg gaatctgttc
    19381 tatcgatttg gctgcatgct aacttggatt ctcatgaatt cattccatca acttattggg
    19441 agaatcaagt agaatctgtt aggaaagctt taccagatgc agtgatttac ttagcagaaa
    19501 tagatcaaga aattattggt tttgctgggg tgattgatca gtttatcgca ggaatctttg
    19561 tgaaaaggga ataccggcaa caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa
    19621 ccaataaaac actgactttg acagtctatc aaaaaaatca atcagctatt catttctatc
    19681 tttcacaagg ttttacaatt caaacacagc aaaaagatga agcagtaaat gaggaggaac
    19741 ttgtgatgaa atggaatcaa aacgctgatt tttgatgaac agaataacga aataatgctc
    19801 ccagcctaat tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg
    19861 aatatgcagt tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag
    19921 ctcaaaaaac ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc
    19981 aaaacgctga tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata
    20041 agcccactag cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa
    20101 gcataacgct gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat
    20161 tagctcaaaa aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag
    20221 gtcagaacgc tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt
    20281 catcgaagta ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa
    20341 taacatcttc attgtctata cctgcatcac ccatataagt gatcgtagca aattcaggaa
    20401 caagtaattt tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt
    20461 cgttttggaa agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca
    20521 ttccgtgaag tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct
    20581 ctgtccaagt gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt
    20641 ttacccaacg gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt
    20701 tggcatacca ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa
    20761 aatcttccaa ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt
    20821 aataaactac atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag
    20881 gagggaaaac tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt
    20941 aagaagaaga tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt
    21001 tggcaagcaa aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc
    21061 attcagcatc aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca
    21121 gaagaaggga aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt
    21181 aatcgagacg aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta
    21241 gtagaaatta ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag
    21301 cgaaaaaaaa cgatctttcc tactgaaaaa gaaaaacatg aaaattacgg acactacctg
    21361 attttagcgc atgcgatcaa tcgggaaaca gaatatgcaa attggctaac agatgtatta
    21421 gaagaaagta accgaaaaaa ataaaaaaat atccgaacag tgatggagaa ttcctttcta
    21481 tatgaaagaa acgcagatca ccgttcggat attcttatgt tcaaacaggc cttttttcaa
    21541 gtatttatgt agcatctact tattaaaaag cttggtactt aggagattag tggcgtttag
    21601 agttctttta acaactgttc gtagtcagct ttttcatcga caggaaggag cttgtaagtt
    21661 aattcgtctg tctcgttgtc atgaatgatg tacatcttag cattctcttc tttgaagtta
    21721 ccgatttgta aaatcgtata gcgttcgtcg atcaattcga tatctttgat tgcttttgaa
    21781 aaaactttac ggacatggtg cattttcttt tggcggattt ctgccttttc tgcagctttt
    21841 gacaaagtaa atccgtcatc taaatatttt ttgatcagtt ctacttttat gactgtaggt
    21901 aaacgatatt ttctaggaca attcgcatca gcttcgatcg aacgaatgaa tcccttttgt
    21961 tcccaatacc ttaattgacg tggagacaca cctacgatat cacttaattc actgatgcca
    22021 acaaccaatt gatcattttc caacaaacgt ttaagggaag ccccaaccat ttttgtcact
    22081 ccttttgtcc tcttgcttcc cttaattata tccgtattaa cttatgtgtc aagtaggttg
    22141 acaaatagcg aatttaggac taaagtattc tagtagtttc agaaaaaatt cagaaagaag
    22201 gagaaatatg agtaacaacc aacccgtaga tatttacgga aaaccatata atcggacact
    22261 tttagttgtt gtcttattaa ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac
    22321 gacagctttt cctacattga tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt
    22381 aacgactggt ttcttgctag tcaacgggat catgatccca ataacagcat ggctgatcaa
    22441 taaattcagt tcaagaaatt tatatctttc tgccatgacg atctttttga tcggaacgat
    22501 tacttgcttt acagcaccta attttagtac attactaatc ggtcgtttga tccaagcttg
    22561 cggtgtggga gtatcgatgc cattattgca aaatatcatg ctatctattt tcccgccaga
    22621 aaaaagagga tcagcaatgg ggatgtcagg gatcgttatt ggacttgctc ctgctttagg
    22681 ccctacatta tctggttata tcattgataa ctaccattgg cgtgacttgt ttggtatggt
    22741 actgccaatc gttgttcttg tcttggtttt agcattcttc ctaatgaaaa gtgttattcc
    22801 attgtcaaat cctagcattg atattttatc cgctatttta tcaacgattg gattcggtag
    22861 tttactttac ggattttcta gtgtaggaga tgacggctgg ggaagcttga aagtaattgg
    22921 tttcttagtt gttggtgtga ttttcatcgc cttgttctca tggcgtcaat tacatttgga
    22981 acatccattc ttggaacttc gtgtattcaa atcatctaca ttcacgattg cagctgtttt
    23041 agctggtgta acaaatatgg cgatggtagg ggcagaaatg gtcgtaccat tgtatattca
    23101 aaatattcgt ggagaatcag cattccattc tggcttgatg ttgttgccgg gtgcattgat
    23161 catgggggct atgatgcctg tgacgggtgc gattttcgat aaatacggcg cgaaacgttt
    23221 agccatcaca gggatgttca ttttgacagc ggcaactgca ccatttgctt tcttgacaaa
    23281 aacgactcca gttttatata tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat
    23341 ggtcatgatg ccggtaacga cttctggtat gaatgcattg ccaaataact tgatcagtca
    23401 tgggacagcg gtaaataata cattccgtca aatcgcaagc tcgattggta cagctatttt
    23461 gatcagtgtg ttgacgaatg tgacaaaagc aaatatccca agcaaatctg tattggataa
    23521 tacgccatta gcttataaag atcaggcaat caatgccaca ctgtcaggtt accatgccgc
    23581 gtttatcgta gcgatcatct ttgggattct tggctttatt attgcctttt tattgaagaa
    23641 aaaagaacct gttctaatga aagcggggga taatgcatga ttattggagt tttgattgtc
    23701 agcttgcttg cttttgcttt aacgaacgtg ttcgctaaaa aaacatggca aaccttttta
    23761 tctttgattt tcggactaat cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc
    23821 aatcactttg gaatggaaaa agtaacggaa acaaaaacag aaaaaattgt ttccagtgct
    23881 gattcccaag gagcagatat gcttttgtat aaagctttag ggaatggaaa agaaaaagtg
    23941 tatctttacc gaacaaatga aaaacaagaa aaaccaaaag caactggtac ggataacgaa
    24001 acaaataaag tagaaaagac agacggcgat gctgaaaaag tgacgaaaac tacttattgg
    24061 gaatacaaaa acgacatgta taaattctgg ttcaatattg ctgataacaa tcacgaatat
    24121 gacagccgag tgaatacatt caaaatccca gagacatggg tagaattaag tacggaccaa
    24181 gcagccaagt tagctgaatt agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa
    24241 gcttatgttc aagacggtat ggtaaaagca atgactgaaa atccaaaaat gagcaaagca
    24301 gagcaagaac aacgtacgaa agccttagct gctgaattcc aacaacaagc attcgcaaaa
    24361 ttagtgaaag aagcaaaagg cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa
    24421 gctgctctgc atcaagcagt aagaacaatc agataaaaat agcactccat atttttcatc
    24481 tgattgggct tagtgccgta gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    24541 gcctgccgtt taactctgag tcacaaggag caatttcaaa aaacagcttc tcatgtttgt
    24601 tgactattgt gacttgtgac cgaggagttg gcaggagaac accgtttatt cgaaaaaagg
    24661 aactgcgaca agacttttgt cacagttcct tctttttttt gctatgctat actagatgta
    24721 aaaaatagga gaaataaaat gagggaaatt tattcttttg aatcaaaaat ctatgcttcc
    24781 gaagtcggca agggcggggc atatattatt tttccgtatg atataagaaa agaattcggt
    24841 gctggaagag ttaaagtcca agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg
    24901 aatatgggcg tgaaaaatcc agatggttca gtctgctaca tccttggact acgaaaagat
    24961 attcgtaaaa acatcggaaa agaaatcggt gatattgtag cagttacagt gaagcaaaaa
    25021 taaaagaggt tgtgacaaaa gacgtgtcac aacctcttga taaacggtgt tcaaaagcca
    25081 gtttctcggt agtaagtcaa atttatgcaa aaatgtggaa acccatgttt acaaaatttt
    25141 tcttactact cgaaacgaag cggctttatc acaacctctt gataaacggc gttcaatcag
    25201 ctgtccctcg gtattagctc aaaaacggca aaatatgaga agctattttt ccattttttt
    25261 ctctcatact caggtcataa cgctgatttc acaacctctt ctttcataat ggtttttcac
    25321 catctttata ataaactggt tctttgatat ggaaatctat tttttccaac caatctaaaa
    25381 tcttgtaacc taactcttct gacatcggaa aagaggcgtt gatttgtgca ttggagatat
    25441 ggtcaacatg gataccggaa gtgatcgtac aacttcccgg aacggcagtt ttgatgatca
    25501 cagctatttt ttcggctaag acatcgtctt tatggaaccg accatcgtgg ctagggaaac
    25561 gtacagtttg catctctgta tcacgagaaa atgtagtgac tgttccgata tgtggtgtat
    25621 ctccgcctgt taaagtgatc aataagtctt taccgattac tttaacagca gcttggatcg
    25681 tgaaacctag tttggttact tcaactatcg tttctttttc catattaatc accctgccaa
    25741 cgttttgaat gctcatttac aatttcaaat tgatccaaaa gtttcattgt atggtgatta
    25801 acaagatctt ggatcgtttg aggctgatta taaaaagcag gaattggtgg tatgatttgc
    25861 acacctagtc gtgctaattt agtcaagttt tccaagtgaa tggcatttaa tggcgtttca
    25921 cgaggaacaa tgactaactt tcgttgctcc ttcattgtta catcagcagc acgtgcaatc
    25981 aggttgtcgc ctaaaccaac agagatacct gcgactgttt tcatacttgc cggtacgatg
    26041 atcattccat cggtcaaaaa tgatccgctg gcgacagcag cacctaaatc tttgtttgaa
    26101 taatggtaat caaacaggct ttcgaattca gttagtgata agtcggattc cagctttaag
    26161 ttctgttttg cccatggact gatgattccg tgtgtttcta tcatcggata ttcttttagt
    26221 ttcttcacta ggttgatggc ataaatggtg cctgaagcgc cacttacacc aatcacgatt
    26281 tttttcttcc gttgtgccaa ttgttccatt ttccgcactc ctttttatga tgtcaaaaca
    26341 gttgcagatc attcattatt tcaaatattt ttcccaatct tctacttctt ggaattttgc
    26401 acgtacaaaa tgttctttca tatcgaaagg aactgtaccg tcaaagatag tttttgcact
    26461 tatcccacgg aaacgaatag agccaggatt atattctggt aattctgatg ggtccaatgg
    26521 atggttcctc attcctggga tcacgatgat atctttatct ccttggaaac gagtgttgaa
    26581 agtccaaact acatcgttca tgtcgaaaat atcaacatct tcatcgacta aaatgactgt
    26641 cttcaattct ttgaaggctg ataaagcaag aatagctgct tgacgctgaa taccttcatc
    26701 cgcttcgttt tctttacgga tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg
    26761 attgtagaca tttactactt ttcctggaat agctttatcg accaaggcaa gaatacttgc
    26821 ttctgtagga ataccggcca tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat
    26881 gattggattg tcttttcggt gagtaactgc tttgactttc actacttgta cagctggatt
    26941 agcatcccca gtatagccag gaaattctgg cattgctttt cctgagttcg tgttgatatc
    27001 ttcttgcatt gtttcatttg gcatgatttc tgcttcaatg atgaattcag cacgagcaat
    27061 acccacttca tcgactgcta taccatcaac gatttgaata ggttcgttac gcaaagctcc
    27121 tgcacaccat agttcattgt agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat
    27181 ggcaattgct ggatctaaac caatattgat agtgataggc attggtttat tcaaggcttc
    27241 gaattgtttt tggaagtgac caatgtggcg tccgccaggc atgatgtaca taccgatcgt
    27301 atctttatct tctagaacca tacgatggat cgttacatcg ctcattgttt tttctggatc
    27361 tgatcctaag actacaccca ttgtgatgta tggaccagca tcttcaggag tatttgtagg
    27421 tgcaggcaaa atgttgcgaa tatcgaagtc tgcatcggta gctagatgaa cgacttcttg
    27481 tgctggagcg tctgctttgt ctactttcac aggtttgatt ggattttcta cagcgtcttt
    27541 caagaaacga ccaagtgttc gataatcatg gtgcaaaatt tcacctacac gtttacgact
    27601 agccattgtt ccgatgttca cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat
    27661 agctggtcct tcttgagtcg gacgttttac tgtaccgcca gcaccgatat aacggtaaac
    27721 accagataat tctgcttcag gatcgatttc aatatctgtt tcgtgatatt gacctggaag
    27781 tgtttttagt tcttcgatga cttttcttaa atcatacggt tgatctgtca tgagagattc
    27841 ctccttagtt gtctttacct atctatagta aaaggtgaaa aagaggaaaa caattcaact
    27901 tgttcggtaa agtattctac aatggaatgg atgcgttttt ttgataatag aataaactat
    27961 gatatactta tcactaaaag aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa
    28021 atggaggagt catttcctaa agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt
    28081 acatttgaaa acggagaagt aaaatacgtg aaatctcatt ggacagaaga gataaccgat
    28141 gccttgcaat ttggaaaaaa aggaagagga aaaagaaaaa atcttctcgc actttctcgt
    28201 aatatgtgga ttgggactga agtaacgatt gaagcagatg gaacagtatt cataaatgga
    28261 aaagacaggt atacgccaga agagttgtgg tacaaaggaa agaaaagtat accagaatta
    28321 taaaaaataa ttaaaaaaaa cagcgcgaaa caaggaacat attacttcct cgtttcttgc
    28381 tgttttctac tatttcagcg ctcttatgac tcaaatctaa atgtggttgt cacaacctta
    28441 ttctgcacgc aaagcgatag cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc
    28501 gccaagcatc gtcaatatag tagagacgat cactagaata atcgcatgga caggatttag
    28561 ttgagcaaca ttttttagat ccgtcatgtt gtataagatg ctattgatag ggaacgtagc
    28621 tagccatgca ataaacacac ctaatatccc agaagaaata cctaaaatac acgtttctgc
    28681 gtcaaatacg cgagtgatgt cttttttacg tgcacctaaa gcttttagta caccgatttc
    28741 ttttgtccgt tcaatcacag atgtataagt aatgatactg atcatgatca tacttgttac
    28801 taaagagatc cctgcaaaag caataaggac ataagtgata gcatccatca atccgccagt
    28861 cagttcagtc atcgttccag ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc
    28921 tttattgtaa tcatctagat aatccaagat cttttctttg tcttcaaaat tgttcggata
    28981 aatcatgata cttgaaggca agctgtctcc acctaaataa ctaagcaatg tttgtttggc
    29041 attttcgtca actttttcag tagttaagac atttacacca ctgtctcttt gagctttgac
    29101 gatttctgat tctttgtttt cgttgacgat ttgagtcgtt aattgatcgc tataagcgat
    29161 tcctggagat aagagattca ttgtagaaga agatttcacg cgtaggatcc ccgagatctt
    29221 caattcatca gaagcattct gatacatcgc atcataatct gtattaggga taaaattccc
    29281 agttggaagt tttgtatagt aagtattgtt gtttactaat ttgaatgttg taccaacgat
    29341 atcatcaaaa tcaagttttt cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt
    29401 gatattagta ttattattgc cgtctacgat cagcacgaca tctgttgctg aggctggata
    29461 acttccggca agtaaagaat aattgtcttt taaaaagttt tctttgctag tgtcaagctg
    29521 tgtagggaaa cttgacactc ccacacctgt cattgctgac atcgtactag ataaagaaag
    29581 agattccgta tcaggatttt gattcgagaa gctgacaggc tgtactttgc catttacatc
    29641 acgtaaaaga ttgattcctg tagtacgggt aaatccgata ttattactta agttaggatc
    29701 gatgtctgtt acgtaatcga cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc
    29761 ttcttcgctg acttttgcag taatcgtttt gctgtctgga taatccgctt tgcttgcacc
    29821 caatccggca tcgtcacgag tctggttagt cgttacctta gaaatagtaa tcggaaattt
    29881 cgccatcgtt tcagcttgcg tgttatcgat ttgtttttgg aatccagaag aaagggacag
    29941 aacgatcgcg attccgatga tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc
    30001 tttttttgtt cggatattgt tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt
    30061 tctgcgtaag ttaaattgat catcttttgg tcgttcgatg tgaggatggg aatctgttaa
    30121 gatattacca tcagaaaatt caatgattcg atctgcgtat tgatgggcca gttcaggatt
    30181 atgtgtgacc atgatcacta atttttcttt cgataactct tggatcaatt ccatgatctg
    30241 gatacttgtt tctgtatcta acgctccggt tggttcatcg cacaaaagga tatctgggtc
    30301 attggctagt gctcgagcga tggccactcg ctgcatttgt ccaccagata actgatttgg
    30361 ctttttatgc atatgatttg tcaagccgac tcggtgcaat gcatcttctg cccgttttcg
    30421 tttttcgtct ttagaaacac cacttaatgt catcccaagt tcaacgtttt caataatgcc
    30481 tagatgacca atcaagttgt aactttgaaa aatgaaaccg atagagttgt ttcggtaggc
    30541 atcccagtca ctgtccttaa agtctttagt agactttccg ttgattacca tatcaccgga
    30601 atcataatta tctagtccac caatcacatt cagcatagtc gttttaccag aaccactagg
    30661 acctaaaatc gctacgaatt ctttttgtct gaaagcgaca gacacaccat ccaatgcttt
    30721 ggtggtggtt tcgccgactt tataatattt ttttatatct tttaattgaa gcataatcac
    30781 ccttctcccg tattgaaata ttcggtatac taataagtat atcagaacaa tgatcgtagt
    30841 atacgaaata aatgttaact gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa
    30901 aacagaggga gatcaatgaa tcgtatttta gtagtagaag atgatccgca attacagttg
    30961 ctttaccgtt ctgtgttgga acgagcgggc ttttcagtca tcaccgcatt gaatggagag
    31021 gatgcgctga aaaaactaga agcgacacgt atagaattaa tcattacaga tatcatgatg
    31081 ccgaaaatgg atggctatga cttgctagac tcattacgta gcaccagatt tgacacacct
    31141 gttctaatga ttacggcaaa ggccgatttt gaggataaga aacgcgggtt caaattagga
    31201 gcagatgatt atatgacaaa acccatcgat gtcaatgaga tgatcctacg tgtggaagca
    31261 ttgcttcgac gttcaaagat caattacagc agtagcttga taatcggaca aacaaaatta
    31321 gagctagatg cttatacagt gttacaagat cagcattcgt tgattctgcc tcaaaaagaa
    31381 tttctgcttc tgtataaatt actgtcttat cccaacaaga tcttcactcg ccaacagttg
    31441 atggatgata tttggggatt agatacgaat acagaagaac gaacgatcga tgtacatatc
    31501 aaacgtttaa ggacccgttt tgaaaagacg gaagattttc aaatcatcac agtacgggga
    31561 ctaggctata aggcggttat ttaaagatga aaaaaaagct attttcacaa ttatggatct
    31621 attttgctag tattgtcttc atttcgatct tagtgacact gctgtgtttt ctcggtttta
    31681 ttttcttatt gtctcgacat tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat
    31741 acccgttatt cgtctttgcg gggttcagta tggtcgtggg tacaggaata tcgatttttg
    31801 ttgggcggcg tatcctgcat ccaatcagtg cgctagggac aaatatgagt ctggtagcaa
    31861 cgggagattt ttcgattcga atggatgaac agcagaaagt agcagaagta cagcaactgt
    31921 ataaagattt caacgttatg gtccaagaac tcaatagtat cgagacactg aggaatgact
    31981 ttgtgtccag tgtatcccat gaattcaaaa caccactagc aacaatccaa ggatatgttc
    32041 aattgttaca agccccaaat ctctctgacg aagaacgcca aatattttta tatcggatta
    32101 tcgaaagtat cacgcagctt tcacaattaa cagagaacat cttaaaattg aacaaactgg
    32161 aaaaccagcg tatccaactg gaaaagaaag agtatcgatt agatgaacaa atcagagaag
    32221 taatcgtttt ttttcagcct aaatgggaga aagaacagct agaattggat atcgaacttg
    32281 ctgcagtaaa ttacacaggc aatgaagagt ttttgtacca agtgtggctg aatatcatgg
    32341 ataacgccat aaagtacaat caaatcaatg gacagattca tatcaaacta tttgagacag
    32401 ccacagagat tgtactggaa gtgaccgatt caggtgtagg aatgaatgaa gagaccagag
    32461 atcgaatgtt tgaaaaattt tatcaaggag atacaagtcg tcaaatttct gggaatgggc
    32521 taggactttc actggttaaa aaaattctag aactgcatga tggacgtatt gattatagca
    32581 gtatcgaagg agtaggaacg acagcaatga tacggttaag taaacaatag gaaaaagaat
    32641 aaaaccccta aaaattctat tgaaggtttt taggggtttt tagtcgtttt tttgtgatta
    32701 gggaaatgtt cttacggttc ttttggcata aaaatcgctg ctttcttatt gaactacgag
    32761 aaatgttaga ggtgtcattg aaacaaggac atgatgcaag aaagagagcg aaagtcatgc
    32821 gtcgctctct ttttttgggt ggatagatgg acagaaaatg tagggcttag ttttcttctt
    32881 gatttaaata ctcccaaaat gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa
    32941 atgcaagata actctctgtt gttggagaaa tagggagaat cacagtattt tcttttaata
    33001 gattcttgac tgttttttcc ataacaatag caatccccat gtcatttgca atcatattga
    33061 tgatcaagtc gattcttgct cctttataag agatttttgg ttcaaatcca gcttcgcgac
    33121 acagatctag tacaggttga tacaagttcg tagtggggcc aaggagcaaa aagttctcct
    33181 ttttcagttc gcttagatcc aatttctttt tactagctaa aggatgattt ttaggtagaa
    33241 ctgctacaaa atcgtctgtt tcagtaggca accactcaat cgaacttggt ggggcctgga
    33301 aacttctagc aaagtggatc gtgtcactat gttcattggc gtcattgtaa gttaattcga
    33361 cactttccac ttctttcaac tggactgtaa attcaggatg gcgtttcaaa aactcggtga
    33421 ttttggaaaa acctttataa ttgatcattg tcggtatcgt taagatattt aatgaaagat
    33481 tgttgtcttt cgtataggtt tcaagcactt cttgcatagc atggtattgt tctacgacat
    33541 tttttacata tgggagcaat aagcttccag cttcggtcaa ttcgattttt ctatgggcgc
    33601 gttcgaataa tttgacgtct aattctgttt ctaacgaaag aatctgttta gaaatatttc
    33661 cttgggtggt gaattgtcga tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa
    33721 gaaaaacttc gaatttatgg atatccatca tattcctcct acttattcca ataaagaata
    33781 ctttaacaca aaaaatgaat tggtgtaatc ttctctgtct gttacaatct ttcttgcagc
    33841 gaaagcaata attacagaaa tttttctgat ttttttcgct tcgtagaaaa agagaggagt
    33901 tttttgatgc caactggagt tttgatcaat gtaggttccg ttttgtttgg cggactaatt
    33961 ggcggattag taggaaataa attaagtgaa cattttaaag cacagctgac gatggttttt
    34021 ggtgtgtgtt cgatggggat gggaatctat tccattgcgc cgatgaaaaa tatgccggct
    34081 gttatttttg ctttagtgat aggaacagcg attggtttga ttgttcattt aggtaatggg
    34141 ataaataagg gggctgcgct gatgcaagta cctatttcta aaatcttccc tagtgaaaag
    34201 cttggtatga cccacgatga atttatcagc acattagtga cggtcattgt tctattttgt
    34261 gcaagtggta cggggatcta tggaagttta gattctggga tgacaggaga cagtacgatt
    34321 ttgatttcta aatctgtcct tgattttttc acagcagcta tttttgcgtg taatttaggg
    34381 tatgtcgttt ctgtgatagc aattccccaa ttcattattt tttatatttt gttcttattg
    34441 gcaaaattta tttatccatt gactacacca gacatgatct tagattttaa agcttgcggc
    34501 ggattcttga tggtcgctac cggttttaga atgatcaacg tgaaaatgtt cccagtagct
    34561 gacatgatac ctgcaatgat cgttatcatg ccactgagct ggatgtggac aaattggatt
    34621 atgccactgt tataataaaa atcaaagaga agccattttt tgctgactaa aaaggatcaa
    34681 atcttttagt cagttttttt gtatttgaca aactgatttt gaaactatat tatatgttgt
    34741 ataatataga ttgaaataaa atattacact tgagaataga aggtgacgta atgcacatcc
    34801 agattccaac tgttcttctt gatggtactg tgctagcagc attaagcaag gaagatatgt
    34861 atgggtatgc cttaacaaaa atcgtacaag aaagtctctc tgtcagtgaa tctacactat
    34921 atccggtttt gagaagattg aaaaaagaag gatatcttga gacctatgat gaaccttttg
    34981 aaggcagaaa ccgtcgatat tactcgttga caccaatggg aagtcgtcat ttagaagaaa
    35041 ttattgatga atggattgtt tttcgagatt ctttagaccg gttattggag gagagcacaa
    35101 atgaatgaat atttgaatga agttgtggat catcttcaag aaattcctga agacgatcgt
    35161 tttgatttga tccagtatta tgaggaatat tttttagatt ctggaaaaac attagatcag
    35221 atcattgatg aatatggtac accaaagcag tttgccttaa aattaaaaat cagttacttt
    35281 tcagaagaag aacaagcaga aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg
    35341 atttggttaa tcattatcgg tttatttgct tctcctgtcc tcattccact agcactgggg
    35401 tttatattgg ttattggggg actattggtt gctttactag cgctcatttt tagtatctat
    35461 gttttatgta tcagtatctt aggtgttggc ctattcatga tcatctcagg aatcagtgtg
    35521 tttggacaat cgattctgag tggattattc tttatcggtg tcggtctgtt cgccacagga
    35581 agcgcagtat ttatttcgcc actgttgctg aaaggaacaa aagggctttt ttatgtcatg
    35641 atgaactttg ttaaatggat gggacgacgt tttattacaa aaagagatct gcattcagcg
    35701 ggcgtttgaa ggaggacaca taaattgaga ttgaaacatt ttttgattac cggtattggt
    35761 ctgatgctta ctggtggcct cctagcaggt gcaacttata cattaggcgc tcaaaagagt
    35821 ctgacttggg ataatggtcc tcggttgatc gatatggtta ctgagacaaa aaaaatcaag
    35881 gatgcaaaca aaatcgtcat ccatgcagaa aatcaaacag tttcggtcat acgcggattg
    35941 gattttgaga taaaaacgac ttatgataag acagaaaagc cagaaataaa ggagaaaaat
    36001 ggcgtgttga ctgtgaatgc agtcaaaaaa tcaacaggtg caatcattag tttagaagac
    36061 acaagtgcac aaatcactat tgttgtccct gcagatcatg aattatctga attgacaatc
    36121 gaagggaata atagtgcaat tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag
    36181 acacatgatg gctgggtatc gttcaatgaa tttacaggag gaacaataac tgcgaaagat
    36241 gaattaggct cacttcgatt agatgaggcc aatgtggata acttgatcgt cacaggtgaa
    36301 aacagcaact tccatctaga agacagtttg atcagcaaag aaggaaaagt catcctaaac
    36361 ggtggcaatt tatctttgca tgagatggaa aacagtggct atactatcca aacgtctggt
    36421 gaatcttata ttgaaaaaga ctaccagcca ataacaaata ctttgttaga acaaggacag
    36481 aaaaagctaa agatcactgc taaagacaca tcgatcaata ttacgacgga tcaagaggac
    36541 aatgaaacag attgatcctg tactcaactg gatattttaa gaaaaaacag caaagatcct
    36601 tccaatcttt gctgtttttc atcgcttaac gcacataact atagtaaaat aagaaagaaa
    36661 atatctaaaa agaaaacagg gtgaaagagt atatgaaggt agaagaaggt caaagagaaa
    36721 agttaaaaac agaaatggaa agattacata cttatatcac tcaactaagc caaaccttct
    36781 atgatccaga caaagaaaaa gtgatggtta attacccgaa caattcagaa ggtcgccaat
    36841 tagagcaagt gtaccatgaa gttttcaaac atttattgac ggtgaagaaa gagctggatt
    36901 attattcttt accgattatc gatacaggaa tattgaagta tgatgggaag aaggaacgat
    36961 ttatctttaa atctgttcgg gaagatttgc ctctttcagc agggatggat ctagaggtct
    37021 tggtagaaga ctattttaca gaagaaaagc actgggttcg aacaaaattg gattatttac
    37081 cgcaggcagc cggagggaca caggcaagcg gttggtacat cactgaagac aaagagttag
    37141 aactagaagg tgtaatggcg cgtattcgaa aaaaaaatta aaaaagtaac attagatatt
    37201 tattgaagga taaaggagcg caggaagtag catgagagaa aaaagagcat tttatatcaa
    37261 tggttatgca gggattgtcg gattggtcgt attagcttta gttggattgt ttttcttcta
    37321 tttaggtatg tggcaagcga aagtttgggc acttttttta agtatttttc tttggttgat
    37381 cacgttattg ttgttaagtt cggcaactgt agtcagtcct aatcaagcaa aagtaatttt
    37441 gttttttgga caatatttag gtacgattcg agaaaatggc tttttcctta cgataccttt
    37501 ggcacaaaaa atgactgttt cgttgaaagt tcggaatttc aatagttctg ttttaaaagt
    37561 caatgatttg gatggaaacc ccatcgaaat ctcagcagta gtagtcttta aagtcgttga
    37621 tacagcaaag gcattattcg atgtagcgta ttatcaagat tttgtagaaa tccaaagtga
    37681 gacagccatt cggcatatcg ctagccaata cccgtatgat acattcaatg agaatgattt
    37741 gacgcttcga ggaaacacga cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg
    37801 tttagcagta gcaggtgtcg aagtaatcga aacacgcttg aaccatttag cctatgcaac
    37861 agaaatcgct agtgccatgc tccaacgtca acaagcaaaa gccattttgt ctgctcgaca
    37921 aaccattgtt gaaggagctg tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg
    37981 acaagaaatc aatttcaccg atgatcggaa agtccaattg atcaataatt tacttgtctc
    38041 aatcattaca gacaaaggaa cgcaacctgt tatcaataca ggcgacatga aagaccgtaa
    38101 gtgacatact ttttgtaaga taggaagaag gagtttagga cggattattg tcttgaactc
    38161 tttttttagt tgttttaaag aaaatgacaa aattgaaaga agaatgatag ttgattcgaa
    38221 ggcagaattg ttcgcttttt tctataatca gtatataaag taagagagaa ggggaaaaag
    38281 tgaaaaaact tctgatcggt ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa
    38341 tcgcagatta cgtagttatg ggtggagaag aatactatgt acaaattacg acagatggta
    38401 caaaaacagt ttcaaaatca gactctggac aagaaacagt caactataga tatatcctgc
    38461 ctggctatac gaaagagggt aaggaaaaag aactggactt caagggacaa ttgactcgac
    38521 cgcttcgtaa aggagcatat ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt
    38581 atgaagaagt cgaaaaaaaa gacgtgccga aaacagcact ggaaaaacta attaaagggt
    38641 agggaaagaa atgaacaaaa tagtcgaagt acagaatgta acaaaagtat acggaaaaaa
    38701 caatgagaaa aagacgcaag cattaagcgg catcagcttc gatgtagaaa aaggcgaatt
    38761 tattgggatc atgggcgctt ctggttctgg taagtcgaca cttttgaata ttttatccac
    38821 attagataaa ccaacagatg ggcatatccg aatcaatcaa aaagatgtga ccacattaaa
    38881 aggaaatcaa ctagcagatt ttcgtgcgaa tgagattggc tttattttcc aagatttcaa
    38941 tttattagaa agcttgacag cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt
    39001 cagaccaaaa gaaatcaagc aacgtgtcca aaaaattgca gaacgtttat ccatctctca
    39061 tattttggaa agctatcctt ctgagatttc cggtggacaa aaacaacggg tcgccgccgc
    39121 acgtgcattg atcacacaac caacgattct attaggggat gagcctacag gtgcgctgga
    39181 ttccaaaagt gcccgggatc ttttagatac gatggacgaa ttgaatacaa aagatcatgt
    39241 atcgattctt cttgtgacgc atgatccgtt ttctgcaagt tattgccaac gtattttatt
    39301 catcaaggat ggcggtatcc atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt
    39361 ctatcgagaa atactatcta ttcttggaaa cttggaacag tagggggaga ttgactatgc
    39421 tttggaaatt atcacttact gggatcaaga gccgtttaag agactacttg gtgttattct
    39481 caggattagt tatggcatct gctatttttt acatgtttga atcaatggca agcaacgaag
    39541 cttttttgaa aagtaatacg atcgttgcgg ctacagttat cattttccga ttcggttctg
    39601 ttttactagg gatcatcaca ttcgtttata ttttatatgc aaattccttt ttgatgacga
    39661 tgcggcaaaa agactatgca atgtttatga tgctgggagc aaaaggcaga aaaatcgcac
    39721 agatgatttt ttctgaaaca ttcttagtag ggatcgcagc tactttagtg ggttcagctg
    39781 tcggtgtagg attgacaagt atcgtccacc gattattagt ggatcagtta gatctcaata
    39841 tttcgcattt tacacctttt agtattcacg ggttgttgat cactgttttg ttttttgctg
    39901 ttttgttttt attagctgct gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag
    39961 cattgattcg tgcggatcag acaccaacaa gaatcaagca gcataaattc ttgtttttcc
    40021 ttgaagtagt actgggaatt gcttttctag gctgcggtta ctatatgatg aaacacgtgg
    40081 ggacctatca agtctttggt ataggggtag ccttagttac gatcgtatta ggaacttatt
    40141 tcattttcca ttctgtgatc attttctttt tatcattact aaagaaatca gaacagattt
    40201 cattgaaaaa attgaacaat ttcacattgt cacagttaaa ctttcgtatt cgtgaatata
    40261 cacaaatgct ctcaatggtc gctatgctgt ttgccttggc actgggcgct ttgacggtag
    40321 gattaggttt taaaaatgaa attgcagact tgacagaaaa caattctgga tatgatctag
    40381 tgatgaataa tcctcgggca gaagatcagc aaaaaatcaa agaactttca ccaacacttt
    40441 ctgcttctta tactcaaaaa gaagatgcgg gaacgattta ctataatgca agtgaatttg
    40501 cggctcaacc attgatcatg ataaaacatg aagatggaac accgccgaag atcacgaaag
    40561 aaaaagttcc tgctaaaaaa atgaatgaac tgactgtaca agaggaactt cgaagctatg
    40621 aattgccgga acagcaagaa aaagaaatca aggtagtcag tcaatcagaa tttgatcaac
    40681 tgaacttgcc acaatccact ttacagctag ttcaggtaaa agacttccaa gcaaacctga
    40741 aaccaatcga aacacttgtt gctcaaaata aaatgaacaa tccatctttg caaaaagtag
    40801 agtacagcaa ccagaaatat gaaatgtatg aagtgtataa tggtctattc tctggttttg
    40861 aatttatggg attcttccta ggtttagctt tcttgaccat gcttgctagc tgtttgatgt
    40921 tcaagatctt gtcaggaagc aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag
    40981 gaacacgacg cgggttgctt aaacaatcga tccgtagaga agtaggcgtg ttattcttag
    41041 caccaggaat cttaggtgta gtccatgtct tattcggatt acaaatgttt ggtctattca
    41101 tgcaaaatcc atacaaagat atttgggttc cgtttacgat cttcttcgta ttgtatttca
    41161 tctactatgt actgacgaca tggttatata ctggaatcgt attgaaaaag agattgtgaa
    41221 aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa aatagctcct catatttttc
    41281 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga
    41341 ggttgtgaca atatttttgt cacaaccttt ttttcacggt cttttattag aatatagtaa
    41401 attttttctc gttactcacg gaataagctg ttaagaaaac ggtttccatg tatactggaa
    41461 tcagtgcaaa tgaaagaaaa gagggtgcaa ggaacatgga aatcaatgaa caaaattttg
    41521 gcgaaaacta tcacttgatt acgatcacga atcaaaatgg gacatctttg tctgtcagtg
    41581 atcttggggc acgggttgtt cattttcaaa caataatcga taatgaaaaa cgagagttga
    41641 tcttaggttt tgatgaagca aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa
    41701 tcggacgtat ggctggacgg attacgaatg gcacattcaa attagatgat gaaacgttcg
    41761 aagtaaaaac agacccggac actggtcatt gtttacatgg tggtgcacct agctttgaag
    41821 ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc attttctatc
    41881 agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa gtacggtata
    41941 cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac cgcaaaacag
    42001 tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca cagtcaatcg
    42061 acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa gatacaacgc
    42121 ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt cctaaaaaat
    42181 taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc atcgatcatc
    42241 catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca agcccagatg
    42301 aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt acagcaaatt
    42361 ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc ggtatcacat
    42421 ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat gtcactctgg
    42481 aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag aaatagaata
    42541 aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa
    42601 ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca
    42661 gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa
    42721 tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa
    42781 aattctaatc ctgtgattgg cacatcgaat cgcttagaca tattgaggat ttcagcgttt
    42841 gcttcttcag ttgttgctgc ttcttttttc ggttcatcat attttgtcag gtcgtacgct
    42901 tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg tagcataacg tccttccaaa
    42961 tatttggttg cttctttgta gtcttttgtt tccgacttcc aagttggttt gtagaaatca
    43021 ggattgccag tcagtccatt tttgatcaag tcggcatagt cttccaaact ttccttgtaa
    43081 gaaggatatt tacggaattg tgcgcggatt tggtagcttt gtccggaact atcttgttca
    43141 agtgtattga aggtcacgct gcttccttta tatgatcctt tgataccaaa aaggttatga
    43201 tttggttcgc ttgataataa gctgtttcca gatccacttt caaggatagc ttgagccatc
    43261 atgactgatg cgtagattcc ttctttttgc ccgatttcct gtgcatcatc tgcaatcttt
    43321 ttcaagaact gatcagttgt ttggttttga ctgaaatgga acgagccatc taatccatct
    43381 aaaatagatg agccgcctgt cagcgaacct gaagcagcag gtaaacctgc taagtttact
    43441 cggacagcaa aatcaggcgt gaaatctgaa atagaagtta ctcgaacttt atctcctggt
    43501 tgaggcgcat ggataaattg tccgttccca atatagatac ctacatggtg agtaacaccg
    43561 gaagaaggtc cccagaagac gagatcgcca ggttgggcct gacttactgg aatctgcgta
    43621 cctgcatatt gctgttcgcc tgtccagcca ccgatataat gaccggttgc tttcatatat
    43681 acgtaataag tcaaaccaga acagtcaaaa gtatttggac cttttgctcc ccatacatat
    43741 ggttttccaa gatgcttgaa tgcttctcga acgattgctt gttggacatt cgttgtattt
    43801 gtaggcaata cagcatcaat tggtgatttt acaacaaaat cagaagaagc agaagatgga
    43861 ttctctatac cgctttgatt tgctgtattt ggacgagatg aagcattgtt cgttccgcca
    43921 ttcgatgaac cattgtttgg atggtttggt tctctctgag tatttccatt gttattcgat
    43981 ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg aattattgtt attattgttt
    44041 gaattatttg aaccattatt attgttgtta ttacccacat ctggtttgtt tggcgtttcc
    44101 ggatctggtg tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc
    44161 ggtccagctt taaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt
    44221 gcatcataat ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca
    44281 gctggcgtaa tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt
    44341 tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc
    44401 ttaccaatca aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct
    44461 gatggatcag ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt
    44521 ccagttgggt cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc
    44581 gtatctgttg tgactgtttg atcagctatt tgattatcta cggcaaatac ggtattggtc
    44641 gacaaaacag acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt
    44701 ttcataataa actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag
    44761 acattttttg gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg
    44821 tattctaaat aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt
    44881 tcattttatt gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta
    44941 tgcatatgta aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat
    45001 cctaaaaaga aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta
    45061 actattcata agacattagg tcttcatcaa acccgctaac gagagtagtg atcattgcta
    45121 cgactagact aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag
    45181 gcaatgtggt cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta
    45241 cagctccacc agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg
    45301 caattccgta aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac
    45361 cggagatcga acgggttttt gaacgtttac ttcgcagaaa tacacctaaa gctgcaccag
    45421 ccattccaaa attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga
    45481 tattactgat catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc
    45541 aaccaccagc gatgattgcg ccactgatcg gtccgttaat atccatcatc caagtaattg
    45601 cggcagctaa accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag
    45661 gaaccgtgat aactaatgaa agtaaaggta caaatactaa ttgcatattt tcgcgaatat
    45721 attttttgag gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga
    45781 cagtagacgt atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc
    45841 cactgtctga aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg
    45901 caatatatcc attcacttta aacgttttgg cagcagaaaa agcaagaaag attggcagaa
    45961 aataaaagac ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa
    46021 gccagccaat cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca
    46081 gcggaatgat cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta
    46141 gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca
    46201 atccctgatc catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag
    46261 ggatttgctg ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa
    46321 gccgagtcgc acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca
    46381 gcatatgaat ctgctcggac agtaccatct gttcaggatt cattgtcgtt tccattagaa
    46441 gaccactcca ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa
    46501 tgcatgacag ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt
    46561 atctggtaac aataatttga ttttttccaa tatttccggg ttcattgtct ttatttcttc
    46621 cgccaattca atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa
    46681 acttggaaga ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat
    46741 gcctgcatcc ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat
    46801 atctgaattg attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta
    46861 gactgctgat tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg
    46921 cacagcaaca gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc
    46981 tggctgaagt gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac
    47041 ggcatccact ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc
    47101 ggcactgcct agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc
    47161 tgcgaccaaa ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc
    47221 atttggtata atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg
    47281 tgcaggattc aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt
    47341 cgcatctaag ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt
    47401 gttttttagg acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact
    47461 attttctcct gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc
    47521 agctatccca gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc
    47581 gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac
    47641 tgcttggttg gcacctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc
    47701 gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac
    47761 tgtaaccgtg ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaattat
    47821 tttttaaaac gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa
    47881 agctatttca agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct
    47941 tcccattctt tttctgggaa ttggatccga tctaacaata acttggcaga tgtttgtccc
    48001 aattcgaaga ttggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct
    48061 atattatcat agccaataat gctgtagtct tcagggatgc tttttccagc ttcctctaat
    48121 ccgcgatata atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa
    48181 tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg agaactgtgt aggtaagagg
    48241 atcagttggt cgtgagggat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga
    48301 gcgtggacat tttccggtgg attttcagga tagacaaggg cgatcgtttg atgtcctaga
    48361 ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca
    48421 ctaaatcctt cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa
    48481 ttttcgttga ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct
    48541 cgacgtgtga gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat
    48601 aatatcgtga cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg
    48661 aagaatggat tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc
    48721 atttgtctag caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc
    48781 tgaacagttt tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg
    48841 attgaaacgc cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc
    48901 atactcctat ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa
    48961 tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc
    49021 gtactcgtgc agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac
    49081 gatcactgca agcatcttca gcaagcacga tatggtaccc gtgttgaaag gcatctaatg
    49141 cagtagcata tacaccgttt gttgtcgcta caccgcttaa aatgatagtt tcgattccac
    49201 gccggcgaag ttgaagatca agagaagtac caaaaaaagc acttggattg tattttgtga
    49261 tagtcaacat attatctgta tctttcaaag aatccttcaa aagtaaatca gtgaattctg
    49321 gaggtacaga agcagtcatt ccacccatat cagtttgttg actcaaataa gggaaagtgg
    49381 agatgtctac gttcactaaa actttcaagg cagcggtacg tttcaatgtt cttactaaca
    49441 aatcattgtt tgtcagaata gatgctgcag tgtgtggaga gagtgtaccg ttatttacga
    49501 tctctttttg catgtcgatc gcgacaaaag cagtttttga taaatcaaaa atcatatttt
    49561 cctttaaatc attcatgatg ttccttcttt caaataacaa tgttttttat gtaagtaaat
    49621 cataaatcgt ttatttgtca actaaattaa aggattcctt tctttagtct agagaaattc
    49681 agtggtaaaa taaataagtg tgagaaaacg cattcgatgc atagatatag cgagaaatat
    49741 cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac
    49801 aaaatgggag aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc
    49861 gaattagtga ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta
    49921 ggtgggatgg atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa
    49981 ttttcagata aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc
    50041 tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct
    50101 cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat
    50161 aactttccgt cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta
    50221 gctaaaaatc ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg
    50281 ggcaagcaag tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg
    50341 atcattacac ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat
    50401 gctaaagtaa ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg
    50461 tagggggatt ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa
    50521 tctaaaggac gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta
    50581 ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca
    50641 ggactttctg atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa
    50701 gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact
    50761 ggcaaaaacg aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta
    50821 caattaactg atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg
    50881 gaagatgaag ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa
    50941 aaagagtata ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga
    51001 ggatacgcga acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt
    51061 tttaaaacag atatttattc tgtcttatat cttgatttta caaacgtaaa gaatttgaat
    51121 acgtacagca aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat
    51181 ttacaagatc gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta
    51241 gaaccggcac agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg
    51301 ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa
    51361 gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa
    51421 gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga
    51481 aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa
    51541 aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt
    51601 tttcctgttt tctttttctt tattgccgca ttgatcacct ttacgacaat gacacgtatg
    51661 gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa
    51721 atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag ggataggtat tgttttagga
    51781 acgatattag gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat
    51841 gatattggga gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca
    51901 ttcttcattg ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg
    51961 cctgctgcat tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac
    52021 atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc
    52081 cgatataagt caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg
    52141 gttgcaggcg tgggattgaa agattctcta agttctgttt cagccaaaca atttggaccg
    52201 atcattgact atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa
    52261 gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa
    52321 accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt
    52381 aaaacagctg atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc
    52441 ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag aactatttga cttatctaca
    52501 ggagatcata tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa
    52561 atcgtgaaaa actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt
    52621 actgatcaat cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa
    52681 gaagaagaaa tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc
    52741 atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta
    52801 atctttgtcg tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc
    52861 aatatttccg agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa
    52921 gaagtcacga tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga
    52981 ggatttggtg taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat
    53041 gtcatctttc cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc
    53101 gtatttacgg ttattgtgat gattgttacc catttcaaat taaagcatat cgatatgatt
    53161 gatgcgttga aatcgaatga ataaaaacag aggttgtgaa atcagcgttt tgagctaata
    53221 acgagggtca gctgctaatt gaacaccatg aatcagaggt tgtaaaaaag ccgtcctgca
    53281 tcgagcaaaa agaatactca aataaaatta gctcctcatg ttttacattt gagaagactg
    53341 attgccgtag ataaaagctt ttgaacacca tttatagaag aaacaagaat attttgctat
    53401 tttaaacaag aggatagaca aatacatgtc tgatctcttg ttttttattg ggcaacagac
    53461 ctcttagaat gtataaagat aaattttaca aacgctttcg gttcagttat aataataaat
    53521 gataacttgt atatataagt tgtggtaggt aataaatcag ttgaatggag gtaaatatcc
    53581 atgacattta aagacaaagt catttaccaa atctatccca aatcttacaa agacagtaat
    53641 ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac tcccgtatct gaatgatcta
    53701 ggcatcgaca tgatttggct aaaccctatt tatccaagcc ctcaaaaaga caacggttat
    53761 gatatctctg attacacagc catcgatcct atctttggta cgatggagga attcgaacag
    53821 ctaatagccg aagcaaagaa ataccggata gaaatcatgc tcgatatggt gctgaatcac
    53881 gtttcgacag aacatgaatg gttcaaaaaa gcactagcag gggaccagca ttatcaggac
    53941 ttcttcattt taagagaaga gccaacggat tgggtatcaa aatttggtgg aagtgcttgg
    54001 tcgccatttg gagatacggg gaaatattat ttgcacttat atgatcggac acaagctgac
    54061 ttgaactggc ggaaccaagc agtaagagag gaactattca aagttgttcg tttttggatg
    54121 gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg ttatcggtaa agacgaacaa
    54181 ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata cggacaaacc aatcacccat
    54241 acgtatctaa aagaattgaa ccaagcaacg tttgggacgg attccactat catcacagtc
    54301 ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt atacagaacc tagtagagaa
    54361 gaattatcaa tggtctttaa tttccatcat ttgaaagtcg attatgaaaa tgggaataaa
    54421 tggtctactc ctccatttga ttttgcagaa ttaaagcgac tatttcatac atggggagaa
    54481 aagatgagtg agggtaacgg ctggaatgct ctatttttta ataatcatga tcagccacgt
    54541 gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac aaggagcaga gatgctagca
    54601 accatgatcc atttgaaccg tgggacgccc tatatttata tgggagaaga aatcggcatg
    54661 atcgacccag actattcatc gatttccgac tatattgacg tagaaagttt gaatgcttat
    54721 aagttactcc ttgaacaagg attcacagaa aaagaagcgt ttcgtcggat caaagcaaaa
    54781 tctcgcgata actctcgaac acccatgcaa tggtcagaca cagaacaagc aggatttact
    54841 acagggaaac cttggttaaa agttggaggc aagctagaag agattaatgt agaaaaagaa
    54901 ctatcttctg aagattctat tttgtcctat tacaagaaat tgattcggct gagaaaaaca
    54961 tatccaatcg ttgcacaagg agattatcat gcttatgaag caaatcatcc ggaagtttat
    55021 ggctttcttc gccaatttga aggacagcag ctgcttgtat taacaaattt ctatgataaa
    55081 gatacaagga tcgccatccc agaaggattc acagaagcca atattcttat cgataattat
    55141 tccgatactg ttcttcatca aagtctggag ctaaaaccat accaggcaat tgctttgctg
    55201 aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc tattctcgta agatcgacgt
    55261 tttgtgtttt atttaaaata atcttcttca gaaaatgaag agttttcata ctctccttta
    55321 tcgccataaa atttattgta gcgttgtttg aatttacgga aaataaaagt aacgagtaat
    55381 ttgactaacg tatagatagg aataccaaaa ataacgccca caattcccat tagatcaccc
    55441 atgacaagta aaacgattaa gatcgtaatc ggatggatct gtaaacgatc acccattact
    55501 cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa accagacaat gacaaatttg
    55561 acagccataa aagtagagtc ttggaatgca atgatcaaac ctggaaagaa ggcgataaac
    55621 ggcccaatat atggaatgat acataacaac ccagcagaaa tagctaaaac actagcaaat
    55681 tctaatccga tcagtaagaa acaaacccag tagactgctc caaggataaa tgaagcaatg
    55741 atttgcccct ttagaaatga acccaattgt tgattagcaa tctttgtcaa atttttaaaa
    55801 tctgtacgaa atgctggtgg gacaatcgct aaaactgact gataaaattt ttgaggatct
    55861 ctcaaaagga aaaaagcgac aattggacca gtaaataaag taataaaaat agctgtaaca
    55921 gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc aataatttcc aataaatcca
    55981 gctatatcag acgtcacgtc gtcaatagaa gcaaaaaatt gattaaatga atcggcaaaa
    56041 ggtgttttct ccaaaaattg tcggagactc tcttgaaaat cttgaaacaa atctggcaaa
    56101 ctactgatca atgtctttgc ctgttcttca agaaaaggaa taagccatat gcttccaagc
    56161 cccaataaaa ataaaataat cacataaacg ataatgactg acaagttcct cgaaaggtgc
    56221 ttgctcaaac atttgatgac tggttccaaa agataataca agatcagtgc aaacaataat
    56281 gggggcaaga tagtagcgat aatgattccg acaggcttga atataaacga gatctgatgg
    56341 aaaaaataaa tgatcagcgc aagtaagata actattccta aaacatagta agaggttttt
    56401 cctcctaaaa agcggatcaa acgattcgtt gtatctgttt cttttttcac tctttttcct
    56461 cctttatcta taacttcatt atagacaaaa gcgggtataa aaggaaaatg atagaggttg
    56521 attgcaaaaa tatcgtcaat cattcatata aactttctca tcaattaaaa taagaaaacg
    56581 cttgacagtt cttttaacca tatgataaat taaatcctgt tgctaaaacg taactattac
    56641 ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag atgagaagag gagtgtcata
    56701 aatgaaacga ttgataggta tattggccat gctggtttta gcaggtatgt taactgcttg
    56761 cggggcaagt gggaaagccg aggacagtaa agaaaaattg agcgtgatga cgacctttta
    56821 tcccatgtat gattttacaa aagcaatcgt cggggatgaa ggagaagtag agttattgat
    56881 tccagcaggg actgattccc acgattatga accttctgct aaagacatgg caaagatcca
    56941 agatacagat atctttgttt ataatgatga aaacatggaa acatgggtcc ccgcaatcca
    57001 aaaaacatta caagaaggca atgtccatac gataaaagcg acagaaggta tgcttttact
    57061 tccgggaagt gaagaaggac atgatcatga ccatgaacac ggggaagaag gccatacaca
    57121 tgagctagac ccacatgtat ggctagctcc aagtttagca atcaagcagg tagcaaacat
    57181 acgtgatcag ctgattgaag cttacccaga aaaacaggaa gtatggacaa aaaatgcagc
    57241 agcctatacc gagaaacttc aagcactcca tcagctttat caagaaacct ttaagcaagc
    57301 aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac tatctagcgt tagaatatgg
    57361 attgaatcaa gtatcgattg ctggtctttc ttctagtgaa gaaccgtctg cggcgagaat
    57421 tgctgaactg aaacactttg ttaaagaaca tggaatcaac tatatttatt ttgaagaaaa
    57481 cgccaaagat agtattgcca gaacgttagc aaacgaagca ggtgtatctt tagaagtatt
    57541 gaatcctttg gaaggattga cgaatgaaca aatagaaaat ggagaaaact atctttctat
    57601 catggaagca aacctcgaag cgttgaaaaa aacgacagag acagaaaacc cgctagaaga
    57661 tactttgacc cccaaaaaag aaaagagcgt ctacaacggt tattttgaag acgaggatgt
    57721 acaagatcgc tctctttcag attgggcagg aacttggcaa acagtagata cgtatgtaga
    57781 agatgggact tttgatcctg tttttgaata taaagaaaaa ttgaatcagg ataagacagc
    57841 acaagaatat aaagaatatt atacgaaggg ctatcaaaca gatattgctt caatcaagat
    57901 cagtgagaaa gagatggcat ttacgtttaa ggatggaaca acgaaacgtt caaattatcg
    57961 ttatgtaggg aaagaaatct tgacctacga ggcaggaaac cgtggcgtaa ggtatctatt
    58021 cgaagcagaa gataaggaaa gtggcgcttt tagatatatt caattcagtg atcacacgat
    58081 tgcaccggct gactccgaac attttcattt atatgcagga aatgaaagtc aagaagtatt
    58141 gctgaaagaa atggagaatt ggccaacatt ttatccagat acaatggatg gacgtgaaat
    58201 tgcacaggaa atgcttttgc actgataggc aaaaaagaac cgaagggttt ggatcggctg
    58261 gtcatttagt cggtccaaac tattcggttc tttttatgaa atcaatttct ttaatccacg
    58321 gatagaggct gggatggtct cgccgtttgc caacgtgact tctctggttt ttttattaat
    58381 cgaccgcaca ttttgtgtat tgataacata ggatttatga caacggataa aattccgatg
    58441 cattttctcg atttctttga tcttctcata gaattgtaat tgggcatgag tcaaatggac
    58501 ttccagttta tgtggcgctc ctagcgaagt agaaaaatat tggatgtctt tgatcggtaa
    58561 aaatttgatc tcatcattaa actctatttt gaagatatct ttttggattt gggacatttg
    58621 aaaatcttcg aaaattttgc tcaggatatg ctcgacattt tcttttaaat cataaagatc
    58681 ttctttgacg atataatcag tcggttcgat gttgcttttt aagattttca tcgacatttc
    58741 cgtatgggaa gtcacataga ttatttttcc taaaggatct aattcacgga tcaatttacc
    58801 gatcgctaca ccatccattt cactgtcttc tagttcgata tctaaaaagt aaataccaaa
    58861 agaagaagtt ctgatcttgg ctgcattgag aagttcttcc ggatcagagg tagaaatatg
    58921 gacataactg tctaagtttt caatcatgat tctatttttg atcaccgttg ataaaacttc
    58981 taattgtttt ggatcgtctt cacaaatata aatgggaatc atatcattac tcctttattt
    59041 tatttgtata taaatcgttt gagaaaaacg ctggttttca attgatgttt caaggaaaag
    59101 ataaggattg gtctttgtca attcatccaa gataaacaag ccaaggcctt cgttttttgg
    59161 atttgttttt gttgagaacc ctttttgttt caactggttt aacggtaatg tatttgttgt
    59221 tgtattgatc acacgaatca tcacggaatg attcttcttg agtatggtca attcgatttt
    59281 tttcttcttt gcagcactag ctccttcaat cgcattgtcc aagataatac caagcatacg
    59341 tacaaggttt accgtatgtt tcgtatccag ttcgattggc tcaggaaaat gaagctggac
    59401 gatcaaccct ttttcctgtg ccatcatgag ttttaatgac agtaaactac ggatctcagg
    59461 aactttcata ttatccaaca tacttagatt ggccggaaaa aggttcatca tctggcgtgt
    59521 aggtaatatc gtttgatcat aataagaccg taatccttct aagtcgcctt ctgaaatata
    59581 gctgtgaaga gaaaaaagca gattttggta atcatgtttg aattctctca agtcttgata
    59641 gtttttttct agatctttga tataatcctg ttgcatcctg acttgttgct ctaataactg
    59701 atcagctaat ttcttattag agattttcca ataataaacc aagatgatca ggccaacaca
    59761 cagatataga atgaaaaaac tcgtattcaa agctaagaca tcaggtgttt caccagaaaa
    59821 acgggtataa aaaataatga tatagtatgt aaataatatc aatgtcccga tcgtaccgat
    59881 catacggcgg tccacagtgt gaaaccaagt ctctaacgat ttttttaaac aaaaagctaa
    59941 taaagcagat aaaaaagcgg ataagacaat gtgaaaatac tgcaatgaat ctcctggttc
    60001 aataaccgaa gatttcaaaa caaaaaaatc gtcgatgctt gttaagtgat ctcctaaaag
    60061 cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt tgacgcgaag tcgaaaagaa
    60121 gaaaaaagta agcatcagca ttcctatagc aattagagca gtaaaaatcc ctaaaatcga
    60181 atagacccat ccaatcaaaa tacaagaaat cagaatgact cccgcaaaca gatatttgta
    60241 tcggatgtga tggtcaatca ataagataca aattgtgtaa atcaaatatt gcaacgctat
    60301 ggaccataaa aacataaagt acagctccca ttgattttta ccaaattata gcacaattag
    60361 cgtattattt ttttaatttt tcagggattt tgaattcaga actcatacca aagcagcagc
    60421 cagatacaaa aaagtcagat aagttagtta attgtttaat gattgttttt ttcatgaaag
    60481 attcctccaa aattcatcaa gtagtttgta ataggaagca acgtagctat ttctaaaaca
    60541 atcccaagta gcaaataggt agaaaagtaa gaagctgaga aaaataaagt aatgagagaa
    60601 aaaattagaa acatgctgat cgccttcttt aataatgggg gacgtttctc gggttcaatg
    60661 ggattgattg ctgtacctct tgatccatag cgaagcagta acaaaaagca gctgattaac
    60721 aaaaagacca tcattgggaa ggagatagtc aattgcagat gtttcatcac caagggaacg
    60781 ccccataaat aaagtagtgt ccacagcaga caaacgaatt cagagtcgga atgcaatcca
    60841 taagcatatc tgcggatgca gatataagaa agaaaggtga ttcctgtttc cgcccatgag
    60901 cctgtcacca gagaaaagac tagaacgacc gctagttttc caagattatt caataaaact
    60961 tctaaggcaa attttacttg gacatacacc atatcttctt ccgatttgtc tccgctgaat
    61021 aaggcttcta ataatttgtt cacaacttta tctttcatct catacgtacc tccaccacaa
    61081 attttatgct aaaaaaaata aaaaacgtct tttgtttcat aaaaagtggt tttgacatca
    61141 aaaccgtcat gatctcctaa aaaaatcttc gttttccaag aaccttacca aaaaaagtga
    61201 cagttttgat acgaaaagta ctttttatga caagtttgtg ctttttcgag tgatttatgt
    61261 taaaaaatta ctgaccttat aaaaggtgat ggagggattc ttatggaaaa tgaacaattg
    61321 tttgaagtcg gcgaaacata tgcatttaaa tctgatttat ttgaatcacc aataaaagga
    61381 aaaatagaaa aaatctatga aaatagtgtg atgatacgtg tcatgaaatg cgcagtagaa
    61441 gacaatgata ccgctcataa tctgcactgg cagttagttg tacgcaaatc aagtacaata
    61501 gaagaataaa aacagataaa atgttgagag cataaagagg ttgtgagtct cccgtttagc
    61561 tctgagttac aaggaataat gttaaaaaac agcttttcat gttttatgaa tattgccttg
    61621 tgaccgaggt gttggcagga gaatactgtt tatttgaaaa aagggctgcg acaagacttt
    61681 tgtcacagcc tttattttca tgaataggag ttacttttgg ctacttttag agaagtagct
    61741 tttaaggagc agataaagtc aaggctttta tttctataaa tgaagtagta aaatgaagaa
    61801 cagattttat tacaggaagg atgccgataa ttgtgatcat tgttgcagga acgatcggtg
    61861 cagggaaaag cacactaact gaaatgcttg cccaagattt agaaacaaaa cctttttatg
    61921 agaacgtgga agataatgaa gtattgccgc tattttattc taatccagaa aagtatactt
    61981 ttttattaca gattttcttt ttgaacaaac gctttttggc aatcaaagat gctttcagcc
    62041 atgatgacaa cgtactagac cgctccatct acgaagatag tatgctgttt catttgaatg
    62101 cagatttagg gcgtgtttct gaagtagaag taaagcagta tgaaggtctt ttagaaacga
    62161 tgctgaagga gttagaagaa atttcgccac aaaagaaacc agatctactg gtttatattc
    62221 gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag aggccgggaa tatgaacagc
    62281 tagaacaaga tcctgaactc tattcctact ataaagaact caatcgacga tacgaagagt
    62341 ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga tggggacaaa tatgatttcg
    62401 tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat caagatgcgg gcaaagaaaa
    62461 tgacagaaga ggcagatgcc cagtcgatcc gctacactac aaataacccg aaagcaaagg
    62521 gaatctttac aactaaataa tataaaaact aagcaaattg ctcactcgca gatatttctc
    62581 tgtgagcagc atcttgctta gtttttttga ttcattgaga tcaaatagtg aaccgattcg
    62641 tatgataaaa ttgccataaa tgaaatgtct gagtgagggc catgatcaaa aataaaatgg
    62701 aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca gaaaacaagg acaatagctc
    62761 ctgcgataaa ggcaagaacg acacttagat agtggaaaaa tcgatcaaaa tctgcttttt
    62821 ctttagaaat acaataatca tataagttcg ttgctacccc ttttaaattt ccagttgtaa
    62881 ataaattagt gtaggcacgc ccgttgattt tatcaaaagc tacccactgg atcgatgcag
    62941 taaaagcaat caaaatcgtt actaatgcgg gatgctcatg gacgggtgtc aaactaacaa
    63001 agaaaaaaat cactgcttca taatatagaa caaacaagcg ccagaaatgt ttttcttttt
    63061 ttctaaaata atcaattaga tactttgtta gaagaatccc taagaaaaag aaaagggtag
    63121 acaatacttt tttaccaaca cttagccatt ctccgtcaaa tgcttggatg atcgatagaa
    63181 tgatattgcc agtttgagca gaggcaaaag caccatattg gatataagta taactgtcca
    63241 tggctcctcc gataaatgtc agcaaaagtc ctattgtacg cccctcgtga aaggaagtct
    63301 gcattttttt catcaccttt tatgaatatt tttgtcttcc ttatcttagc ataaacgatt
    63361 gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg cttcccattt tcatttttag
    63421 aaaggtctgt tagtataaag aaaaaataga caacgaaact ttaaataacc aacttgaaat
    63481 aaacaaattc aaagaagaaa gatgggaagg aaatcaatca gttgaatgaa aaagaaattc
    63541 tccgattaca aaaagaattg aaacgctggg agaacaagta taaaaagctt caacaagtct
    63601 ttcggctaca agaagaaatc attgctacct atgaagcggc ttatggcgaa gcacccggac
    63661 ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa cagctctttc tttaaaatga
    63721 tggagaaacg ttcctataat cgcttcaatc aagaacaaaa agcagctatc acatatgata
    63781 tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg caaaacgcaa acaatctgtg
    63841 caaaagctgt ttatctgatg acgcaaaaac aagtagacga agaacgcata ctaatgatca
    63901 cttttacaag gaacgctgca aatgaactaa aaaaacgagt agataatttc agccagagaa
    63961 aaaccgctgt ccatatcgga acattccata gtatcttttt taggatatac aatgaaattt
    64021 gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg agacttttca aaggatactg
    64081 cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa atataatctt tatatatttg
    64141 atcaatatgg agaaaaaacg attgcttctc ggttagacta ttggcagaat atgaaccttt
    64201 cagtagaaga aatggttcag ttagttaaag aaaaatatga ttccattgat aagaatgcac
    64261 gacagccgat atcagaacga ctgtatggat tgctcacgga actccaagaa caaaaaagaa
    64321 gccagcaatt gctagagttc aatgatgtac tccaaaatct gaaaagcgct ttggagaatg
    64381 aggagatcca gcattatatc ggccaaaaat atgattatct attcattgat gaatttcaag
    64441 atactaatcc acttcaatgg gaaatcgtga aacggatgac aaaggaaaac aggatcaagt
    64501 tgattgttgt tggagatgat gatcagagta tctatggatt cagaggatca gagccagcat
    64561 acatcaagaa ctttgaaaaa gaatatccta ccaaaactct tttcttatta actaattatc
    64621 gttctcgggc agctattgtc caaggagcaa atagattgat cagctataat aaaaatgatc
    64681 ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg tgttatggaa gcctatttgt
    64741 tttctgatac taaagcagaa agtcagtggc taatcaaaca aattcagaca tttattcaaa
    64801 aaaatgggaa atataaggaa tcaatcatat tataccgatc agcttcacaa acgcagcagc
    64861 ttgtccaatc tctgctgaaa acgaatattc cttttgtact ggaagcagat agtccatatg
    64921 aaggtatctt tggtatcaag tctttccaac atttctatca aaaaattgtt cattggcaaa
    64981 cagcactgaa tctgcaaaag aaaaatcagg cctaccagca attgctccgg cagctgatgg
    65041 cagactgcta cctgaaaaaa agtgaatgcg atcaatactt ttctcctaaa tatcaaaata
    65101 tagccgttgc agattatatt ttgtcgataa gccccaattt aaaatcgaaa tctgcagaaa
    65161 tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca tcaagaaatc tattcgcttg
    65221 ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat cgaccagagt gatcaagaat
    65281 ggatacgaga agaagtaaag cagcatgcta ctttcagaag actccaagcc ttatcgcaag
    65341 aaacccaagc gacagctaaa gaattaaaaa cccgcttgat tgcttacaga caaggaaaac
    65401 ttgatgcact atgtatccag agcattcaca aaagtaaagg tcttagttac cgaaatgtct
    65461 ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg agcgacagaa aagaaagtaa
    65521 tcgactttaa tgaaataaaa gcagaaccag tgacgacgat agaagaagaa cgaagactat
    65581 tttatgttgc gatgactaga gcaagaaatc gtctgtatct atgtgtacct caaatgaaaa
    65641 attcaagaaa attgaaagta tcgagattta tcaaagaaac cgggagtcgt gtgaaaaaag
    65701 gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt tcaagaaaga agaaggaatg
    65761 tacgaaaatt gcttctcaaa tttttgtgaa atttcagctt attttcgaag aagctatttc
    65821 tggaacacag tttattcgga aaagggaggt gtgacaagaa t
//
LOCUS       DADJYG010000003        62076 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.003, whole genome shotgun sequence.
ACCESSION   DADJYG010000003 DADJYG010000000
VERSION     DADJYG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.003"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            119..2902
                     /locus_tag="JZ382_000116"
     CDS             119..2902
                     /locus_tag="JZ382_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426331.1"
                     /translation="MKKTKTVSHYGSVVFMVAVLFLSQLAGLSQITFAKTESEKVVAD
                     EHANIVIDYEMDEELKQIQWSMTYEKHAKDEAEYQMQFKLEIVEHEGLLLRESIQEIE
                     RTFGKVGPVDEEWYYAGDFSSENERIEIVFKSHIVEEYNHYHLKITPRLMKQTEDELV
                     EVLIGDQEEYTVVVTLPENITASETQETSEPSPEISKEVESAEIPIDDSIEESKAEAE
                     EVGVYAAPNMSDPTLNGAYDDAIPLNSLFTIPDYLTIVRNVPNFVDNNKSIQMTPNHI
                     LTALYNRGHMVSKSQNTIRFSNLTRDIRFTSYMYFDTDTEGLSFFLHDGNPTSLLHSR
                     RGIHNKYDPTVVMNHDAKALGAYKHKDPIGLFDIDRHMANAMVLEFDNQVSTGPLGNR
                     DKFGDQGLSGAHIAVAPSDSPATKTLIKDFETNNAFVTGSWWQLDVNITPTGILRFLM
                     TDLRDGGYGTTTGEININDYFSNLQSKNLYWGFAATATYTKDNYTTLFMTEPNFDIQG
                     ELVHEITMQGQGGAENVSGQTVAADTELEHTVTFYNEEGDGSIFLTPSNDNGLSILTS
                     DLEESKSNPFKFTDMVPISEYQPYFTYQTISNNDEIIEHRVNAMYDDSLQRFYPIETI
                     EIEVGTVLKLNYKTKVKAEFPGYEDHDVYERVSFRGRSLSPTGSYYDTQVEQAVQYAI
                     QREPNTPPELANLRTVDGQMAEKTTFTDFKERFRFAFDYGDGDLDRLYYSVWINGEVL
                     TENQMLLNAREELQTLRSAAFTIDIKDLEGVFQRGENTITVTLTDNRIDEPLILEESF
                     QVEGYLGFEFVTEEYSWKYARSQLPMNRQAQAREERMRVVTRNTLNTSDSYKVTVSAE
                     ELNHVDMQHAISEGYLVFKNEMGEIALSALTLEIDKVYEFSQEEGLLLRLNSQDRTGV
                     YSGTITWKIEDVL"
     gene            2977..3273
                     /locus_tag="JZ382_000117"
     CDS             2977..3273
                     /locus_tag="JZ382_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426332.1"
                     /translation="MKSKNRYWMISVLLLLLFSLSMPVVNAATQAGETHAVIEINERE
                     IGKQTEKGEEKQKSSFPEANEAKAGLISFVGTLLIIGVILVYRHQIRGGEKHEK"
     gene            3263..3835
                     /locus_tag="JZ382_000118"
     CDS             3263..3835
                     /locus_tag="JZ382_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426333.1"
                     /translation="MKNRFSLALWSALFIGMFSCSSAVHGQTEGATSLKIVPLNEQFP
                     RMLYIDDLLFVSRYTDEHNQIHRESNDLSITFLDSRKESTEWQLQLKTEDFRCKKDGH
                     LLTADYQLGRGMFSGKNEEAVKGLEAFEYDHQDAGKEYRTIIQSTGNKERGIVTYTIP
                     GEQSTLTFPEGTSSGHYYSKHYWRFVNAEF"
     gene            3835..4482
                     /locus_tag="JZ382_000119"
     CDS             3835..4482
                     /locus_tag="JZ382_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426334.1"
                     /translation="MRKKGGGNRKKLVGVLSTSAMLFTCFAPTVMAAEFGEPMDGTTR
                     VEITDFLVVDDLPTYPGIFTLDDVPDIDFGEHTVAQISANTTVNGTFDGALQASDFRP
                     LEASKTEALELISEYTGVEDTTELTAAWENAVVAGDWVLTVSATNLTDDIASALTING
                     ENVLESATTLPISYVSGTFDHINELTSEAPTLTVKANTPVGEYTGTVTYTAVDSI"
     gene            4589..5617
                     /locus_tag="JZ382_000120"
     CDS             4589..5617
                     /locus_tag="JZ382_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAY2426335.1"
                     /translation="MRRHLVLVLITLLIQLVFPGIVVFADENHGADYSVHPILPDNQR
                     EGRSFFDLRVEPGTKQTLQIQINNFSDQEQTYFINVNTAQTNGNIVIDYGQNELPEES
                     IVQQPICTLVDYPKEAVIPGNKAGVVTLEVQAPDTSFDGILLGGIQVKKDFSAEKEES
                     NQPFFSEYAYVVGLMMSANDKEVVPDLKLNEVRSEVITNNAGVVAVLENPQPINIKGV
                     TVEATVHSKGQEEPIMTRKIENGGIAPESVFAMPIYHGEAGNTKPLESGDYQLLVEAQ
                     DETGQKWLLTKDFTITKEQANSVNHEVFTVENNTNSLLYIIIGALAAVLLVGGFYVYR
                     RGKKFRRR"
     gene            5926..7398
                     /locus_tag="JZ382_000121"
     CDS             5926..7398
                     /locus_tag="JZ382_000121"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY2426336.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFSHGYAVFAGLERLVNYLENLTFSDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDSEGNMVDTIKLSSNAEKVTTPGKKQVWRITRNSDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPVLNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMATMERMKKSVAELHTEA"
     gene            7410..8234
                     /gene="nadE"
                     /locus_tag="JZ382_000122"
     CDS             7410..8234
                     /gene="nadE"
                     /locus_tag="JZ382_000122"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY2426337.1"
                     /translation="MSLQTEIIHELGVKPTIDPTEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDESYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVEISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(8309..9199)
                     /locus_tag="JZ382_000123"
     CDS             complement(8309..9199)
                     /locus_tag="JZ382_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAY2426338.1"
                     /translation="MQLQINEESLPVYEALASKTRIRIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKIGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFVDPRRMDARILWFTSGFVEYQSPNFLNAE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSDITINDIPKDQDTWHLRIEVKEDAEHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            9396..10913
                     /locus_tag="JZ382_000124"
     CDS             9396..10913
                     /locus_tag="JZ382_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAY2426339.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NESGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIASRPKRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRCSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAAETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTIK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAMYGQGEVLSPQIHSA
                     NYATEDFEEVPYLESVAVINDKELVIFAVNRGEEEMNLTVRLSELSVAGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCYLSDEGGTKNER"
     gene            10903..12174
                     /locus_tag="JZ382_000125"
     CDS             10903..12174
                     /locus_tag="JZ382_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2426340.1"
                     /translation="MKGKKWLIGSLLVVATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAVELGQVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPQLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAIYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPEKLMNDADDAINQLLQK"
     gene            12349..13245
                     /locus_tag="JZ382_000126"
     CDS             12349..13245
                     /locus_tag="JZ382_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2426341.1"
                     /translation="MNGFTKYWNRPERAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCVLTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            13259..14122
                     /locus_tag="JZ382_000127"
     CDS             13259..14122
                     /locus_tag="JZ382_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2426342.1"
                     /translation="MAPESSITVTVNKRKAVSGKKIIRTISMLIILFVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            14128..14691
                     /locus_tag="JZ382_000128"
     CDS             14128..14691
                     /locus_tag="JZ382_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAY2426343.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGKESGHIIYFFFQGMKQHFGKNLFLSMLTTVIYFLVNQLLEEQTIGLSISGY
                     VVSLIYSMFLLSWVYVISENKDTSFLQIFEQSTWKVFFDFLRNILCCALLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            14701..16224
                     /locus_tag="JZ382_000129"
     CDS             14701..16224
                     /locus_tag="JZ382_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAY2426344.1"
                     /translation="MDEEIKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPSHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWLDTIGRKERRPKKIDLAWRGIE
                     PNSFGIHEFFKWISHVQAEPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEIGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWKNPTYYVMQHLSKYAK
                     GQVLEQQIICPVYDCEKIKKVPYVDSVVVDNGNQLTIFLINRTNEQQNVTIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            16236..17195
                     /locus_tag="JZ382_000130"
     CDS             16236..17195
                     /locus_tag="JZ382_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAY2426345.1"
                     /translation="MMKTLYNNPIVQQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAESLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHMDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEIAGNS
                     NLYLSEMANPWTLKGEQLLLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLNESWHKLSEPVFVSSEKNKQFGPGHNSFTISEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYDL"
     gene            17281..18360
                     /locus_tag="JZ382_000131"
     CDS             17281..18360
                     /locus_tag="JZ382_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2426346.1"
                     /translation="MAKYQDIADEIKQKILHEEYEVNTTIPSEFKLQEIYAVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REKGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTKDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LLLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQVQPNILYEPVLVERETVKENEK"
     gene            complement(18519..19415)
                     /locus_tag="JZ382_000132"
     CDS             complement(18519..19415)
                     /locus_tag="JZ382_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY2426347.1"
                     /translation="MIIVYFCTYMSSGYFQEYNKKRLFCTYISYRMRLLTKSDQPPSR
                     NSRRREYEAEQEFEQIQLNVKQEKTTVTSWHTLFLEKYRSLVIAGIGVAVFQQFQGAN
                     AIFYYIPLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSV
                     MGLSFILPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGR
                     ASGLASSFNWIGSFLVSLLFPVMTASMSQEIVFSIFGIICFLGVLFIQEIVPETRGKS
                     LEEIEQSASKKTYPKRISIHHS"
     gene            19479..21140
                     /locus_tag="JZ382_000133"
     CDS             19479..21140
                     /locus_tag="JZ382_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAY2426348.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQAILNKEEHVREI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLVEKEHVAID
                     LDHILPKVLVAGKEAGKLSESGAYLIDPTGNLKPGIPICPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSEDVQIDRLVGHGGIFKTPEAGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMIDQHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMSRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            21097..21795
                     /locus_tag="JZ382_000134"
     CDS             21097..21795
                     /locus_tag="JZ382_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY2426349.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQHK"
     gene            21811..23235
                     /gene="araA"
                     /locus_tag="JZ382_000135"
     CDS             21811..23235
                     /gene="araA"
                     /locus_tag="JZ382_000135"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY2426350.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVEQLFEEYKELYTFDYGDYKEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            23542..24702
                     /locus_tag="JZ382_000136"
     CDS             23542..24702
                     /locus_tag="JZ382_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAY2426351.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSTYVGRELQGKTIGLLGLGAIGTKVALSCYGLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLINQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYVTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNKNELV
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERS"
     gene            24820..26679
                     /locus_tag="JZ382_000137"
     CDS             24820..26679
                     /locus_tag="JZ382_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY2426352.1"
                     /translation="MKRYISRLSTKQKKILKGTVAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACFVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVLGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKAKIKEITEKYQKIADEKNKAVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQILGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYNGKYDRLGYSAEISDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGKLEKKTTQDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            26789..27223
                     /locus_tag="JZ382_000138"
                     /pseudo
     CDS             26789..27223
                     /locus_tag="JZ382_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27660..28682)
                     /locus_tag="JZ382_000139"
     CDS             complement(27660..28682)
                     /locus_tag="JZ382_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAY2426353.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELSSLSLAVEENSPTY
                     LAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDETRQLLYGANY
                     HKGEVNVYHLLGDGELKAADSVFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYDKET
                     GDFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVSEDGSHIQN
                     KQIISTEGDFPRDFALDPSNGFVVCANQNTDNLTLYRRHPETGLLELLQKDVYAPECV
                     CVYFDR"
     gene            28810..29163
                     /locus_tag="JZ382_000140"
     CDS             28810..29163
                     /locus_tag="JZ382_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY2426354.1"
                     /translation="MIQATITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTITFDDQSYTIDYVGPMANGNLETVAHVSLIFDTCPEEGQIANGIYLHP
                     HELPAIGLGSQIIYN"
     gene            29175..30341
                     /locus_tag="JZ382_000141"
     CDS             29175..30341
                     /locus_tag="JZ382_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY2426355.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIVW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKRLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAIIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIVGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(30430..31050)
                     /locus_tag="JZ382_000142"
     CDS             complement(30430..31050)
                     /locus_tag="JZ382_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY2426356.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKETHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTEQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGINQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(31240..31536)
                     /locus_tag="JZ382_000143"
     CDS             complement(31240..31536)
                     /locus_tag="JZ382_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY2426357.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKKHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTASGFRAVYIIGGMEALQSSVA"
     gene            31741..32391
                     /locus_tag="JZ382_000144"
     CDS             31741..32391
                     /locus_tag="JZ382_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2426358.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKSPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            32407..34134
                     /locus_tag="JZ382_000145"
     CDS             32407..34134
                     /locus_tag="JZ382_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426359.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKEATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISEMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVETADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            34131..36023
                     /locus_tag="JZ382_000146"
     CDS             34131..36023
                     /locus_tag="JZ382_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426360.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMVVAPRSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQVIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            36228..37097
                     /locus_tag="JZ382_000147"
     CDS             36228..37097
                     /locus_tag="JZ382_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAY2426361.1"
                     /translation="MRDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGKIKKLNRFSLIIPSTEELADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREVPETMDQEALLRKSEEKFSKLSA
                     GSYFEKIHLNVTDLAKERQFLNRVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            37305..38330
                     /locus_tag="JZ382_000148"
     CDS             37305..38330
                     /locus_tag="JZ382_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAY2426362.1"
                     /translation="MYPQTKQLILKKMEEGFYPGVAAAFIKENQVESFTAGFAQVVPE
                     KQPMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLSEFENQTITLRHLL
                     THTSDIKTWIPNRDELSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEKIYQE
                     TAVEIFQEQVLEPLEMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLNDSITFVQMYLNKGKISNGRFLEEETIDSLLQDQTPERNGYRSLGWDLKK
                     DVGDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLQK"
     gene            38369..39319
                     /gene="manA"
                     /locus_tag="JZ382_000149"
     CDS             38369..39319
                     /gene="manA"
                     /locus_tag="JZ382_000149"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAY2426363.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEQVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKNGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGIASFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPSGVSEWTVQGELAIIASEPGK"
     gene            39412..41115
                     /locus_tag="JZ382_000150"
     CDS             39412..41115
                     /locus_tag="JZ382_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY2426364.1"
                     /translation="MEYKWKANSNSRVFTKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     VMKGVSGDLHATLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSD
                     APQIVGAMIIGPLAGISYEQFVSRFHAKFKGGYEMLVSNLLVGLIGSIICIIGIVFIE
                     PALNSFHLLLVSVVSWLVKMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSYLFLMEANPGPGLGILLAILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMVGGASGTLIFQWLDAGLATPVSPGSLLVIFANTPYPQLAGVSIGIAISTFV
                     TFICATLLLKLPKKEEGDNTVKERGEILELSTIHSVVFACDSGMGSSAMGAALLKKKL
                     AAEKIDLAVGYSSVYELNNQVNQLVIVQHELADLAREKVPQSQIFVLNHFLETDILLS
                     RIKKAIHEPNWKEKKERVEVIEQKKSPVNVVFLYKNNRRGSQTMGMAILSQLAKKEKK
                     QFVILKEPIEQLKIQEETIYIAQRELAVNEQLKEKIPQLVLVEHWVATNEYEKLVKGE
                     K"
     gene            41117..43150
                     /locus_tag="JZ382_000151"
     CDS             41117..43150
                     /locus_tag="JZ382_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAY2426365.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVTIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKVARGSFVIEASEEVMEQLKENTEGKSDDLPTAKRQHAILAELLLT
                     TEPLSLAHFMELYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIVGPEKYRRLLTA
                     NVLELEINEYELFHSIPFDSSLNYFFRFVDPQHLSLARQVVGEELIQKKTNLSDRKLE
                     HLVLMLTLTMDRVTKNHLLTNETYNGLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEIANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESDISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIVDLKKIDLQE
                     EYDIVISTSILPGYDGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKNTYNSNNEWML
                     EDTYEETMHFMEQINQLLDRFFIQELDNPNTVSETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLVSKPIFCIFNLKQPLTIDGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            43161..43601
                     /locus_tag="JZ382_000152"
     CDS             43161..43601
                     /locus_tag="JZ382_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2426366.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(43711..44838)
                     /locus_tag="JZ382_000153"
     CDS             complement(43711..44838)
                     /locus_tag="JZ382_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426367.1"
                     /translation="MTLFTFERNLLLKNKVNFLFPLLLIVLFAFPLFFDHELAYTEFD
                     ELNHNYEETQRLIETLKEDENEKEFVESLEKSNKLIEAILRAKNTGNVQEMVEATYHY
                     EKDILGRLISGQRQGLPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTVYYGAAMLS
                     LVIPFLFVSILVVIKNGLGDFRYPVGTIIGQEIRILPMYAYLFQSFLFLLLWVFFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIVGG
                     DIFSPLASEQVTFMNGILTLGTWSIVLLFIGMCAIYIKKSY"
     gene            complement(44841..45518)
                     /locus_tag="JZ382_000154"
     CDS             complement(44841..45518)
                     /locus_tag="JZ382_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426368.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLDLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLTAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNRLIRQWQEEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRQLYEV"
     gene            45782..47548
                     /locus_tag="JZ382_000155"
     CDS             45782..47548
                     /locus_tag="JZ382_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY2426369.1"
                     /translation="MERMIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDTLTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVADAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKVAKQASKGAQGIPQGTALSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPNARF
                     VSVENFLNSPRYDEIIQELSSKQEPSTSETKNEPTEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQELKGEPDSLIVAKESLAEKAQMAAPQSIHL
                     SVNSMVNPQKFESIAQYLKQTA"
     gene            47563..48000
                     /locus_tag="JZ382_000156"
     CDS             47563..48000
                     /locus_tag="JZ382_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2426370.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQKLVEAGCVDEEYIDAMV
                     ERDNMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVNFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            48013..49164
                     /locus_tag="JZ382_000157"
     CDS             48013..49164
                     /locus_tag="JZ382_000157"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY2426371.1"
                     /translation="MRAVHFGAGNIGRGFIGEILATNGFEITFVDVNHTIIDALNERK
                     EYEIELADESKERITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQAREMEGNTTPLDVIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKEIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDSEQHHAYIEKII
                     HRFQNKYISDAITRVARTPLRKLGYQERFTRPMRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(49226..49924)
                     /locus_tag="JZ382_000158"
     CDS             complement(49226..49924)
                     /locus_tag="JZ382_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HAY2426372.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYVAEKMGVSCHTEIV
                     SIHEPFDANKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     KGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNEKLAARLIRLALEQRY
                     GEELDLPYLEGQTA"
     gene            complement(49917..51269)
                     /locus_tag="JZ382_000159"
     CDS             complement(49917..51269)
                     /locus_tag="JZ382_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAY2426373.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNHG
                     "
     gene            complement(51492..52892)
                     /locus_tag="JZ382_000160"
     CDS             complement(51492..52892)
                     /locus_tag="JZ382_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAY2426374.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     LPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSSAKLPFRFSLFTKLAVLAFVILFVSGTILIYLLEKDHLF
                     QDSSMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIVEVTSAFGTTGLSLGITGDLSVVGKITIAALMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            53053..53790
                     /locus_tag="JZ382_000161"
     CDS             53053..53790
                     /locus_tag="JZ382_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAY2426375.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYADITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPSEFTKMVLTNHLEDEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFDEN"
     gene            53915..54394
                     /locus_tag="JZ382_000162"
     CDS             53915..54394
                     /locus_tag="JZ382_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAY2426376.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTSS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            54391..55158
                     /locus_tag="JZ382_000163"
     CDS             54391..55158
                     /locus_tag="JZ382_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426377.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEETINNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKINELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLSSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEQVVYSRSQRRRHS"
     gene            55332..55901
                     /gene="lepB"
                     /locus_tag="JZ382_000164"
     CDS             55332..55901
                     /gene="lepB"
                     /locus_tag="JZ382_000164"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY2426378.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDKFKKEFSEDKLQGEYAYSSGFQAQAESASTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKII"
     gene            complement(56023..56625)
                     /locus_tag="JZ382_000165"
     CDS             complement(56023..56625)
                     /locus_tag="JZ382_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2426379.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMVEEKIQAADLKNVRTIHADASVEFPAI
                     QADVILLSLVLLHIPDTKTILIKLFELLAPGGQLIIVDFDKNEQISHPKVHNGFIQEE
                     LNDRLKKTGFVSTASHTFHRGEKLFMNKNASLFLSISQKA"
     gene            56946..57530
                     /locus_tag="JZ382_000166"
     CDS             56946..57530
                     /locus_tag="JZ382_000166"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY2426380.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKREAMPIFEETDVFDVIQNMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGK
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            57533..58606
                     /gene="prfA"
                     /locus_tag="JZ382_000167"
     CDS             57533..58606
                     /gene="prfA"
                     /locus_tag="JZ382_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY2426381.1"
                     /translation="MYDQLQSIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGENVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            58599..59438
                     /gene="prmC"
                     /locus_tag="JZ382_000168"
     CDS             58599..59438
                     /gene="prmC"
                     /locus_tag="JZ382_000168"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY2426382.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQQLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPDVPLEVIDIGTGTGAIAISLKAARKNWYVSAVDLSEEALEVAKENAQHEG
                     TKISFYHGNTLEPVQDRRFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     VYEKIAVEAPSVLKPNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYS"
     gene            59632..60657
                     /locus_tag="JZ382_000169"
     CDS             59632..60657
                     /locus_tag="JZ382_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY2426383.1"
                     /translation="METKRYDETELKEAAKALKAGELVAFPTETVYGLGANALLPNTV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPETEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRMPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVFHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVREGDWSTAVQWAKNKKIRVGVIASPEISDQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60823..62067
                     /locus_tag="JZ382_000170"
     CDS             60823..62067
                     /locus_tag="JZ382_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY2426384.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDETLAKKINSAVFPGIQGGPLEHVIAGKAAAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGLKEEDAVKVAELVVKALQAKGDN
                     AQLDEVKTGVRELTEKFPLHKK"
BASE COUNT        20619 a        10816 c        13044 g        17597 t
ORIGIN      
        1 gtgtttgcaa tcttatttta attaagttaa attttagtta tcggataaaa gatgagaacg
       61 actatatcgg agctttttga aaaaatctca ctaatattca agaaggggaa gagaggaaat
      121 gaaaaaaacg aaaactgtct cgcattatgg cagtgtagtt tttatggttg ctgtcctatt
      181 tttatcgcaa cttgcgggac tgtcgcagat cacttttgcc aagactgagt ctgaaaaagt
      241 agtagctgat gaacatgcga atattgtgat cgattacgaa atggatgagg aactgaagca
      301 gatacaatgg agtatgacat atgagaagca tgcaaaagat gaagctgaat atcaaatgca
      361 atttaagctg gaaatagttg aacacgaagg attattactt agagaaagca ttcaggaaat
      421 cgaacgtacc tttggaaaag taggaccagt tgatgaggaa tggtactacg caggtgattt
      481 ttcttcggag aatgagagaa ttgaaatcgt ttttaaaagc cacatagtag aagagtataa
      541 tcattatcat ctaaaaatca cgccaaggct aatgaaacaa acagaggatg aattagtaga
      601 agtcttgatc ggagaccaag aagaatatac tgtcgtggta actttaccag aaaatatcac
      661 agcatctgaa acacaagaaa caagtgagcc ttctccagag atttctaaag aagtagaatc
      721 agcagaaatc ccaatagacg attcaataga agaatcaaaa gcagaagcgg aagaagtcgg
      781 agtctatgca gctcctaata tgtcagatcc tactctaaat ggggcctacg atgatgcgat
      841 tccgctaaat agtttgttta cgatccctga ctatttaacg attgtacgaa atgttccaaa
      901 ttttgtggac aataacaaaa gtatccagat gacgccaaat catattttaa ctgcacttta
      961 caatcgagga catatggttt caaagagtca aaatacgatc agattttcaa acttgacaag
     1021 agatatccgg ttcactagct atatgtattt tgatacggac acagaagggc tctccttttt
     1081 cttacatgat gggaatccta cgagtttgtt acacagccga cgaggaatcc acaacaaata
     1141 tgatcccact gtggttatga accatgacgc aaaagcactg ggtgcctata aacataaaga
     1201 tcctattggt ctatttgaca ttgatcggca catggcaaat gcaatggtcc ttgagttcga
     1261 taatcaagta tctacaggtc cattaggaaa tcgagataaa tttggagatc aaggtctttc
     1321 tggtgcacat attgcagtgg ctccttccga tagtcctgct accaaaactc ttataaaaga
     1381 ttttgaaaca aataatgcct ttgtgacagg tagttggtgg caattggatg tcaacattac
     1441 tccaacaggg atccttcgct ttttgatgac agatttacga gacggcggat atggaacaac
     1501 aacaggcgag atcaatatca atgactattt ttccaatctc caatcaaaaa atctttattg
     1561 gggatttgct gcgacggcaa cttatactaa agataattac acgacattgt ttatgacaga
     1621 gccaaatttt gatattcaag gtgaactagt ccatgagata acgatgcaag gacaaggggg
     1681 agcagaaaat gtgagtggtc agacagttgc tgcggataca gagttggaac atacggttac
     1741 tttttacaac gaagagggag acggtagtat attccttact cctagtaatg ataatggtct
     1801 ctcgatttta acttctgatc tggaagaaag taaatcgaac cctttcaaat ttacggatat
     1861 ggtcccgata agtgagtatc agccttattt tacttatcaa actataagta ataatgacga
     1921 gatcatagag catcgagtaa atgcgatgta tgatgactct ctacaacgct tttatccaat
     1981 agaaactatc gagatagaag taggtacagt attgaagttg aattataaaa caaaagtaaa
     2041 agcagaattc cctgggtatg aggatcatga tgtctatgaa cgagtatcct tcaggggaag
     2101 aagtctttct ccaacaggca gttactatga tacacaggtg gaacaagccg ttcaatatgc
     2161 gatccagcgt gagcccaata ccccaccgga attagcgaat ctaagaacgg tagatggaca
     2221 aatggctgag aaaactacgt ttactgattt caaagagcga tttcgttttg cttttgatta
     2281 tggtgacggt gatttggacc gtttgtatta tagtgtgtgg atcaatggag aagttcttac
     2341 cgaaaatcaa atgctcctca atgcacgaga agaacttcaa acacttagat cagcagcatt
     2401 cacgatcgac ataaaagatc tagagggggt ctttcaacga ggtgaaaata cgattactgt
     2461 aacgctcact gataatcgca ttgatgaacc gcttattttg gaagagagtt ttcaggtgga
     2521 aggttatctg ggatttgaat tcgtgacaga agaatacagc tggaagtatg ctcgatcaca
     2581 attgccaatg aacagacaag cacaagcacg tgaagaaagg atgcgtgttg ttactagaaa
     2641 cacattgaat acatcggatt catataaagt aaccgtatct gctgaagaac taaatcatgt
     2701 ggatatgcaa catgcgatta gtgaagggta tcttgtgttc aaaaatgaaa tgggagaaat
     2761 cgcactttct gctttaacac tggaaattga taaggtttat gaattttcac aagaagaagg
     2821 cttgttactg cgcctaaaca gtcaagatag aacaggagta tattctggaa cgatcacgtg
     2881 gaaaattgaa gatgtgttat agcaagaagc tagctttgaa agggggggat cgtatggctg
     2941 gctagatact agaaaaaaag tggaaaggag aaaacaatga aatcaaaaaa ccgatactgg
     3001 atgatttctg ttcttcttct actcctgttc tcgttatcga tgccagtggt gaatgctgct
     3061 acccaagctg gtgaaacgca tgcagtcata gaaatcaatg aacgggagat aggaaagcag
     3121 actgaaaagg gagaagaaaa acagaaaagc agttttccag aggcaaatga agcaaaggca
     3181 ggattgattt catttgttgg gacgctgttg attatcggcg ttattttagt ctatcgtcat
     3241 caaatacgtg gaggcgaaaa acatgaaaaa tagattttcg ctagcgttat ggagtgcatt
     3301 attcatagga atgtttagct gttcttctgc tgttcacgga cagaccgagg gtgcgacttc
     3361 tctgaaaatc gttcctttga atgaacaatt tccacggatg ttatacattg atgatttact
     3421 gttcgtatcg cgctatacag atgaacacaa tcaaatccat cgagagagca acgatttatc
     3481 gattaccttt cttgattctc gaaaagagag cacagaatgg caactacaac tgaaaacaga
     3541 agacttccgt tgtaaaaaag atggccatct tttgactgcc gattatcaat taggcagagg
     3601 aatgttcagc ggaaagaacg aagaagctgt caaaggattg gaagctttcg aatatgatca
     3661 ccaagatgca ggaaaagagt atcgtacgat catccagtca acaggaaaca aagaacgtgg
     3721 aatcgttaca tatacgattc ctggcgagca gtcaacactc acttttcctg aggggacatc
     3781 tagtggtcat tactactcga aacattattg gcgatttgtg aatgcagaat tttgatgaga
     3841 aaaaaagggg gaggaaatag gaaaaaatta gtaggggttt taagtacaag tgcgatgtta
     3901 tttacatgtt ttgcaccaac agtcatggca gcagaatttg gtgaaccaat ggatggaacg
     3961 actagagtgg agattactga ctttttagta gtggatgatc ttccaactta cccagggatt
     4021 tttacactcg atgatgttcc tgatatcgat tttggtgaac atacggtggc gcaaatcagc
     4081 gcaaatacaa ctgtcaatgg tacttttgat ggcgcattgc aagcttccga ttttcgtccg
     4141 ctagaagcca gcaaaacaga agctttagaa ttgatcagcg aatacactgg tgttgaagat
     4201 acaacagagc tgacagcagc ttgggagaat gctgtagttg cgggagactg ggtattgaca
     4261 gtcagtgcaa caaacttaac agatgatatt gcttctgctc ttacgatcaa tggagaaaat
     4321 gtattagaat ctgcaacaac tcttccgata tcttatgttt ccggtacatt tgatcatata
     4381 aatgagctca catcagaagc gccaacacta acagtcaaag cgaatacacc ggttggagag
     4441 tatactggta cagttactta cacagcagtg gattcgatct aacagcagta tttcaagtaa
     4501 aataatttgt tttacttgta aacaaccgca aaaggatgga gcggataagc taactccatc
     4561 ctttttacat aaaatggagg gatgaaaagt gagacgacat ttagtattgg ttttgataac
     4621 tttattaata cagctagttt ttcctggaat cgtcgttttt gctgatgaaa atcatggagc
     4681 agactatagt gtccatccta ttttaccgga caatcagcga gaaggacgtt cattctttga
     4741 tttacgagta gaacctggaa caaaacagac attacagatc cagatcaata atttttctga
     4801 tcaggagcaa acgtatttta tcaatgtcaa tacagctcag acaaacggaa acatagtgat
     4861 tgactacgga caaaatgaat taccagagga gtcaatcgtt cagcaaccca tctgcacatt
     4921 ggtcgattat ccaaaagaag cagtgatccc agggaataaa gcaggagtcg ttactttaga
     4981 agtacaagca cctgatactt cctttgatgg aatcttatta ggcgggatcc aagtgaaaaa
     5041 agatttttca gctgaaaaag aagaaagtaa tcagcctttt tttagcgaat acgcttatgt
     5101 ggttgggttg atgatgagcg caaatgacaa ggaagttgtg ccggatctga agttgaatga
     5161 ggtacgttcc gaagtgatca ccaacaatgc tggtgtggtt gctgtgctag aaaatcctca
     5221 accaatcaat atcaagggag tgaccgttga agcaactgtt cattcaaaag gacaggaaga
     5281 accgataatg acacgaaaaa tcgaaaatgg tggtattgct cctgaatcag tttttgctat
     5341 gcctatttat catggggaag ctggaaatac gaaaccttta gaatcggggg attatcaact
     5401 tttagtcgaa gcacaagatg agacagggca aaagtggttg ctgacaaaag actttacgat
     5461 tacgaaagaa caagcaaact cagtcaatca tgaggttttt actgtagaaa ataatacaaa
     5521 ttctttgttg tatatcatta tcggggccct agcagcggtt cttttagtcg gtggtttcta
     5581 tgtatatcgc agaggaaaga aattccgcag aaggtgattc atatcgaata ttaagtgaaa
     5641 tctggtttat aagaagtagc aaaattaaga gtaggggctg tgacaaaagt cttgtcgcag
     5701 cccttttttc cggataaacg gtgctcaaaa gcttgcatct gcggtaataa gcccaaccaa
     5761 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct
     5821 ttttcacaac ctctttttca gaaaataatg aagatttctt ttcctatcaa ttttttttgt
     5881 tataatgggt aggaaatgaa aacttgccaa gaggagtaat acaacatgaa taatacttac
     5941 ccagacgaca gtttgacgct gcatacggat ctatatcaaa tcaatatgat gcagacatac
     6001 tgggaattag gtcgagctga tttacatgct gtttttgaat gttattttcg agaaatgcct
     6061 tttagccacg ggtacgcggt ttttgcagga ttggaacggt tagtcaatta tttagagaat
     6121 ctgacgttta gtgattccga tattgcttac ttacgtggct tagaagtcta tcctgagggt
     6181 tttttggagt atttacaaaa ttttgaattt aaagcaacgg ttcgttctgc tcgtgaaggt
     6241 gaattagttt ttgcaaatga accattgatc caagtggaag gtcctttggc acattgtcag
     6301 ttagtagaaa cagcactgtt gaatatggtc aatttccaaa cgttgatcgc gacaaaagca
     6361 gctcgcatca aatctgtgat cggagaagat ccgctcttgg aatttgggac tagacgagcg
     6421 caagaattgg atgcggcagt ttggggaact agagcggcat atatcggcgg agcagatgca
     6481 acaagcaatg tacgtgctgg aaagatattt gggataccag ctagtgggac tcatgcgcat
     6541 tcgttagtcc aatcttatgg aaatgattat gaagcattta tggcgtatgc taaaacccat
     6601 aaagactgtg tgttcttagt cgatacttat gatacgttga aatcaggtgt gccaagtgca
     6661 atccgcgtag caaaagaatt aggtgataaa atcaacttcc aaggcgtacg tatcgacagt
     6721 ggagatatgg cctatatatc taagcgtgtt cgggaacaat tagatgcagc tggatttacg
     6781 gaagcaaaaa tttatgcttc gaatgactta gatgaagcaa cgattttgaa tttaaagatg
     6841 caaaaagcta aaattgatgt atggggtgtc ggaacaaaac tgatcaccgc ttatgatcag
     6901 ccagcccttg gtgcagtatt caaactagta tcaatcgaag atagcgaagg aaatatggtg
     6961 gatacgatca agttatcaag caatgcggaa aaagtgacaa ctccaggaaa aaaacaagta
     7021 tggcgtatta ctcgaaactc tgatgggaaa tctgaaggag actatgtcac attatgggat
     7081 gaggatcctc gagaagaaga agctatcttt atgtttcatc cagtgcatac gtttatcaat
     7141 aaaactgttc gggattttac cgctcgcccc gttttacaag atatctttat cgaaggaaaa
     7201 agagtatacg aactacccgt gctaaacgaa atcaaagaat atacaaaaga aaatctagat
     7261 tcactgtggg aagagtacaa acgagacttg aatccgcaaa aatatccggt cgatctatct
     7321 actgaatgct ggaaccataa aatggctacg atggaacgaa tgaaaaaaag tgtagcagaa
     7381 cttcatacag aagcatagga ggagaataaa tgagcctgca aacagaaatc atccatgaat
     7441 taggtgtcaa accaacaatt gatccaacag aagaaattcg taaaagtatt gattttatga
     7501 aagcttattt gaaaaaatat ccgtttctta aaacctttgt attaggtatt agcggtggtc
     7561 aagattctac tttagcaggt cgtttggcgc aactaacgat ggaagaaatg cgtgaagaaa
     7621 cgaaagatga aagctatcaa tttatcgctg ttcgtcttcc ttatggtgaa caagcagatg
     7681 aagaagatgc aaaagctgct cttgacttta tccagcccga tgttagtctg cgagtaaata
     7741 tcaaacctgc tgttgatgca caagtacaag tattaagtga agcaggtgtg gaaatcagcg
     7801 attttaacaa aggaaatatc aaagcacgac aacgaatgat tacacaatat gccgtagccg
     7861 gagaaagagc aggagctgta ttagggacag atcatgctgc agaaaacatc actggttttt
     7921 tcacgaaatt tggtgatgga ggagccgata tcttaccatt gttccgtttg aacaaacggc
     7981 aagggaaaca attgcttcaa gcattgaatg caccggaaaa gctatacaca aaaattccga
     8041 ctgcagatct tgaagatgga aaaccaatga ttgcggatga agtcgcacta ggtgttacgt
     8101 ataacgaaat cgatgattat ctagaaggaa aagaagttcc cgctcaagca caagaaaaaa
     8161 tcgaagcgtg gtggaacaag acgcagcata aacgtcattt gccaatttct gttttggatg
     8221 atttttggaa ataacaataa aataaaaaaa cagccgtccc ttcataggga ttggctgttt
     8281 ttgctttata aaatagaaaa gaaaactatc agctgtagta aacttttaat ttgatatctt
     8341 ggtcgtaatt accgaatcct ttaccaaata aagtacagcc acctacatgt tcggcatctt
     8401 ctttcacttc gattcgcaaa tgccaagtat cttgatcttt agggatgtcg ttgatcgtga
     8461 tgtctgacaa aggttcccca ttcatatttg tcaaatgttt ggtgattcgg atcgtcttca
     8521 gcaagccgta ttgattaaga ttatctggat accaatcagg cgtatatttc ccgcggatat
     8581 cagcaaaatc tccagggctc gtccatgttc ctagctctac gccatttagg ctgaaagtga
     8641 tatcggaagg ccaattgtca tttgaaaaag gaaattcaga agaaatttcc acagaaactt
     8701 ccaacatttc taatgtatcc tctgcattca aaaaattagg tgactgatac tcgacaaacc
     8761 cgctggtaaa ccagaggata cgagcatcca tccttcgagg atccacaaaa tagcgtggtt
     8821 catccacttt tccgataaag tcatggatcg tcgctaaacc acatgtcggt tcaattgcat
     8881 aattcgtata gtgaccaata ggtatcgatg tttctttcgt gtcaaaagca ttgaatattt
     8941 tttctgggaa actgatatct attttatcga cacgaaggct cgaaattttt tgttgtccta
     9001 ttttctctgt tttgatgatg ttcgcttctt ctaacttttt cacgtgcatc gtcgtgatcg
     9061 cgctgcttac tccaagttct tttgccaaat ctttgacatt catcttcttt ttggaaagca
     9121 actgaatgat cctgattctt gtcttgctag ctaatgcttc atataccggt aaggattcct
     9181 cgtttatttg tagttgcata tatatcaccg cttaaaaggt ttttcttcta tattaacata
     9241 tttattaatg aaagctatat tgaattattt gatttaatca tgattaatat ttacattaat
     9301 tataatgggt aaaaataaat agatttaaca tttttattat tgacaacgct ttcaacgggg
     9361 tgtattattc atgtataaat taaaggaggt cagttatgga agccacaatg ttgattagta
     9421 gtgtcaagcg tgtaggcaaa gttgatccgc ggttgtatag ttcatttatt gaacatatgg
     9481 gacgagcggt atatgaaggg atttatcagc cagatcatcc atcagctaat gaaagtggat
     9541 ttcgccaaga cgtcattcac ttagtaaaag aattgaacat tcccatgatt agatatcctg
     9601 gtggaaattt tgtgtcagga tttcgttggg aagacagtat tggtcccata gcaagtagac
     9661 ccaaaaggtt ggatttagct tggcggacaa ttgaaacgaa tgaagttggg attcatgaat
     9721 tttataactg gtgtcagcat gtaggggccg aaatcaatat ggctgtcaat ctgggtactc
     9781 gtggggtaga tgcagctcgt aatttagtcg aatattgtaa ttttcctaaa ggaacttatt
     9841 ggagtgattt aaggtgttcg aatggtcaag agagcccttt tggaattaag acatggtgtt
     9901 tagggaatga aatggacggt ccttggcaga ttggtcataa aacagcagaa gaatatggac
     9961 ggttagcagc ggaaacggct aaggcgatga aactcgtaga tgattcaatt gaggtggttt
    10021 tatgcggaag ttctaatcgg caaatgccta catttggtga ttgggagtta acagtccttg
    10081 atcaagcata tgatcaaata gattatttgt ctcttcatca atattatggc aaccaaaaaa
    10141 atgatctttc tcattatctt gcacgttcct tggacatgga tcggttcatt tctgaagtga
    10201 cagcgatgtg cgatacaatc aaggcgaaga aacatagtaa gaagcagatc aacctgtcct
    10261 ttgatgaatg gaatatttgg tatcattcag cggaacaaga tgagcaagca gcgccttggc
    10321 agattgctcc tcctttacta gaagataact ataattttga agatgcatta ctattaggct
    10381 gtctattaat cactttattg aaacatgcag acagagtaaa gatagcttgt cttgctcaat
    10441 tggtaaatgt tattgcacca atcaggacag aaacaaacgg agaagcttgg agacagacga
    10501 tttattatcc atttatgcaa gctgctatgt atggacaagg agaggtgtta agcccacaaa
    10561 ttcattcagc aaactatgcg acggaagatt ttgaagaagt gccgtattta gaaagtgtag
    10621 cagtaataaa tgacaaagaa cttgtcatat ttgcagttaa ccgtggagaa gaagaaatga
    10681 atctaactgt tcgcttgtct gaattatcag tagcaggagt gattgatttt tctgaaatga
    10741 gtgggtttga tacgaaagca accaatcttc ctaaaagtga acaagtaaaa ccaagtcatt
    10801 caacgaatgt aaaagctgaa gatgggaaaa tcaattgttt gttgaaaccg ctatcttgga
    10861 atgtagtcag atgttattta tcagatgaag gaggaacaaa aaatgaaagg taaaaaatgg
    10921 ttaattggct ctttattggt agttgcaaca ggtattttag gtgcttgtgg tggcggaagt
    10981 tcagcagaat ctgattctga taaagaagtg actttgactt tttggaatgg gtttactgca
    11041 tctgatgggg aaatattgca agaaatcgtt aatgaattta ataaaacaaa tgataaaaac
    11101 atcaaaattg aaatggatat catgacttgg gccaatttga atgaaaaact tcctacagcg
    11161 atttcttcta aaaaggcacc tgactttatt gcattaaatt atccagattt tgcacagtat
    11221 gtagaaaacg gagcggtaca accattagac gatttctggg aatatgatgg agtagataaa
    11281 tctgatttta aagaaacggc tgttgaatta ggtcaagtag aaggtaaaca gtattttgtc
    11341 cccatgcagg tacaaggaat gtatatgtat tggaataatg atttgtttaa acaagctggt
    11401 ttagacactg aacaaccacc tactacttgg gaagaattga cagaaatggc accacaatta
    11461 actgatagta gtaaaaatgt atcaggtttt gtcttcaata aggatggaac tgcaccatta
    11521 tataactgga tgttagcaaa tggcggtaaa ttagtgaacg atgattatac taagtctgaa
    11581 tttgcctctc ctgagaacct taaaacgtta aaagcgatcc aaaaaatgat tcatgtagac
    11641 aagactggcc cagaatcgat ttctggcgcc gaaatggaca atttaatgaa tgctggtcaa
    11701 ctagccattg aaatcaatgg gccatggtta aataacggat tgaaagcaaa cgagattgat
    11761 tataatgtga ctaccttacc acaagggagt gactcggaca aaaaaacagc tattttagat
    11821 ggcgtaggat atgccattcc ggcaagtaca gataataata aaaaagaagc tatctatgag
    11881 tttttgaaat attggaatac aactgaaatt ggtaaaaaat ggagtatcga aaatgggttt
    11941 cctgcctact tggattcagt agcaaaagat gaagaagtca aaaatgatcc tattgtttct
    12001 gaattatcga aacaaatgga ctatgcagag ccatatttac caggttttac ccaaatctct
    12061 gcgattaata acgatatcat caacccattg attgaaaaat tattagcggg agaagaccct
    12121 gagaaactca tgaacgatgc agatgatgcc atcaatcaat tattgcaaaa atagtacaga
    12181 cctttatata ggatagactg ttaacaagat acagatatct gaatgatcga cgtagtgttt
    12241 gcagataggt gttattgcag aaatcacagc gatcgttcgg atatcttatt cagattcgat
    12301 taaaccaagc tcgtatattt tatcaaaatg aaagatgagg tggactatat gaacggattc
    12361 accaaatatt ggaatcgccc tgaacgtgct gcgtatttgt ttttgactcc atctttttta
    12421 gttttattat tatttactgt cataccgctt ttaggaacat ttgctattag ttttacagat
    12481 ttgaatattt tctttacatc aagaaatttt gtaagttttg aaaatttcac acgtatattt
    12541 agtgatgaac gagcagttaa ttcgttgatt cattctttat attttaccgt acttgaaacg
    12601 ccaacacaaa ttattgttgg tttattagca gcagtagcat tatctaaaaa taccagattc
    12661 aatcggtttt gtcgttcaac gttttacatt ccagttattt gttcattgac gtcaattgcg
    12721 attatttttt ctatgttact tgatccgaat gtaggggcga ttccttactt gttttcacaa
    12781 gtcggcttgc cgattcctca attttttcgc gatccaactc ttgccatgcc aactgtagcc
    12841 atcatgactg tttggaagaa ttttggtgtt actatgacga ttctcttgac tgctattcag
    12901 ggaatctctc cctctttgta tgaatctgct caaatggatg gtgcaacaaa tcggcagcag
    12961 ttttttaaca ttacactacc tcagatcgtt ccctccttag gtttttgtgt tttaacgaat
    13021 ctgattggtt cgatgcaagt atttgaccaa gtatatgttg caaccgctgg tggcccacaa
    13081 tttaagacag aaacagctgt tcaatatatt tacagtcgag gatttacagc accttatgaa
    13141 ttgggctatg catcagcatt gtcttcggtt ttgtttatca ttgttgcaac tatagctatc
    13201 tccactaatg tgtacatgtc acggaaagaa aaacaaatga aataggaggg aactctgtat
    13261 ggcaccagaa agctcgatta cagttactgt gaataagagg aaagcagtta gtggtaaaaa
    13321 aatcattcga acgattagta tgctaattat attgtttgtt attgcttttc cttttttgtg
    13381 gctgattatt tcttctttta aacacgaaaa agacattatc tcatttccgc ctagaatatt
    13441 tgcagacagc tataccctag ataactatat taaggtttgg acaacgatcc ctttattaga
    13501 ttacatcaaa aatacagtaa tttttgctgg agggacagtg attacttctg tattctttga
    13561 ttctcttgcg gggtacgcat ttgctcgtat gcgtttcaaa gggaaatcag ttttgttcta
    13621 tttcgtttta ctgacaatga tgatcccatt ccaagtcttc atgattcctc tatttattga
    13681 agtcaatttg cttggcatgc ttgatactta tgcaggttta atcattcctc gtatgacaac
    13741 tgcatttggt atcttcatga tgcgctcctt ttttatcact ttaccagatt ctttggaaga
    13801 ggcagcacgc attgatggac taagtgagtt taatattttt ttgaaaatca tgttgccatt
    13861 gagcaaacct actcttttat cattagggat tttcacactg atgaatagtt ggaatgactt
    13921 gctttatcca ttgatattga caagtagttc aaaaatgaga actttaccag cagggctagc
    13981 tttatttacg gggcagaaca tttctttcta tggacctgta atggctggaa cggttatttc
    14041 aatgttgcca ttactagtgg tttatatttt tgcacagaaa tattttgttc aaggaacagc
    14101 tatgtctgga atgaaagagt gaggaagatg aaaatagttg agacaaaatt ttatggacag
    14161 ttgattgtgg tttctgatta catcttatta ggaattctct gggtaatcgt gtgtttgccg
    14221 gttattacta tcgtgcctgc tacatgtgct gttttttatg taatggcgca atggaaaggt
    14281 aaggaatcag gacacatcat ttattttttc tttcaaggaa tgaagcaaca ttttggtaag
    14341 aacttgttcc ttagtatgtt aacgaccgtt atttattttc ttgttaatca attattagaa
    14401 gaacagacaa tcggactttc aattagtgga tatgtagttt cacttattta ttccatgttt
    14461 cttttatcct gggtatatgt gatttctgaa aataaagata cttcgtttct acaaattttt
    14521 gaacaaagta catggaaagt attttttgat tttttgagaa atattctttg ttgcgctttg
    14581 ttattaggtt ttgtactact catatttctt tttcctccgt ttatttttat ctttgctggt
    14641 ggcgtttgga aacttgtatc tattatcttg actataggaa atgaaaggta gggaaagctg
    14701 ttggacgaag aaataaaagt aaaaatcaga gaggatttta cagtaggaga gatcagttct
    14761 cggctgtttg gctcatttgt tgaacatatg ggaagtgtcg tctatacagg gatttatgaa
    14821 ccgagccatg aaacagctga tggtgacggg tttagacaag atgtcctctc ttatgtaaag
    14881 gaattggaat taggtgtcat tcgctatcct ggagggaact ttacatctgg ttatgattgg
    14941 ttggatacta taggtaggaa agaacggcga ccaaaaaaga ttgatttagc atggcgaggt
    15001 atagaaccaa attcttttgg aatccacgaa ttttttaaat ggatcagtca tgtacaagcg
    15061 gaaccgatat tcacagtgaa tttaggaaca aaaggaatcg atgaagcaaa aaatttagta
    15121 gaatattgta attttccaaa aggaaccttc tggagtgatt tgagaataaa aaacgggcaa
    15181 aaaaaacctt ttggcgttaa attatggtgt ttagggaatg agctagatgg gccatggcaa
    15241 gtgggtgcta agagtgctta tgaatatggt aggttagcaa atgaagcctc gaaagtcatg
    15301 aagttagtag atgattcaat tgaaacaatc ttagttggaa gctctacgcc acgtttacca
    15361 tcgtaccctg aatgggatcg tatcgcatta gaagaagcat atgagaacgt agattattta
    15421 gctttgcata actatattga caagtatgat gaagaagact tgactaaacc tcctaaaaat
    15481 gagcgtgatg atacagcgac atacctggca aaagcttatc gattcgatcg tcaaattgaa
    15541 gagatcgttg ctacttgtga ttatgtaaaa ggatatttga gaagtgagaa aacaatgtat
    15601 ctagcgttcg atgaatggaa cgtacatgcc caaccggaaa aaagacacag ggattttgaa
    15661 atcggtagtc ccattgattg gtgttacttt actatggagg atacattatt atttggttca
    15721 ttaggattgg ctattttacg acatgcagat cgcgtaaaga ttagttgtca atcattgtta
    15781 gtaaacacaa tacctttaat actaacagat gctaaaggcc aagcatggaa gaatccaact
    15841 tactatgtga tgcaacactt atcgaaatat gctaaaggtc aagtattgga acaacaaatc
    15901 atttgccctg tttatgattg tgaaaagata aaaaaagttc cttatgtaga cagtgtggtc
    15961 gtagataacg gcaatcaact gactattttc ttgattaatc gcactaatga acaacaaaat
    16021 gtaacgattg aacatgattt taatttgcta gatcaaggag agcactacac attgaccagt
    16081 agtgatctat tggacaaaaa tacacgtgaa gaaccagaaa agataaaacc tgaaataaac
    16141 tatttttcaa tagaaaaaca tgacgagtta aatgtaggga tagaaaaata ctcttggaat
    16201 gtaattagaa taaagaaaat gtgaggaaat ttgttatgat gaagacactt tataataatc
    16261 ctatcgttca gcaacgggca gatccttggg tatataaaca tacagatggt tattattatt
    16321 ttacaggatc ggttcccggc taccaagtga ttgaattaag aagggcaaag tcactgaatg
    16381 aattagaaca tgctgaaagc ctgattgtat ggcaggctca tgaagaagga ccgatgagtg
    16441 aattgatctg ggcacctgaa gtccattata tcaatggaaa atggtacatt tactttgctg
    16501 caagcaatca taaaaccata cgtgatgaat cccatcatca ccgcatgttc gtacttgaaa
    16561 atcagcatat ggatccaatg aatacagatt ggatagaaaa gggacaaata aaaacgaaaa
    16621 gggaaacttt cagtttagat ggaacaactt tctcttctca gggaaagcta tattacgtgt
    16681 gggctcaaca agatcctgaa attgctggga attctaatct ttatctatcc gaaatggcta
    16741 atccttggac gttaaaggga gagcaactcc tcctttctat acctgagtat gaatgggaaa
    16801 aacggggttt ttcagtaaat gaaggtccag cagtccttac tcgtaatggg aaaatattca
    16861 ttacgtattc tggtagtgca acggatgaaa actatgccat gggtctgctg tgggctgatg
    16921 agcacaagaa tcttttatta aatgaatcat ggcataaatt atcggagcca gtattcgttt
    16981 cttctgaaaa aaataagcaa tttgggcctg gtcacaactc gtttacgatt tcagaagatg
    17041 gatgctttga tattttgatt tatcatgctc gaccagagaa gaaccaaaaa ggggatccat
    17101 tagacaatcc caatcggcac gcgaacgcgc aatgctttac gtggaatgag caaggatttc
    17161 cagaatttgg tgaacctgta ccgtatgact tatgagaaca ataaaccttg atttttgctt
    17221 gaataaacct tacagactcg ttatgatata tgtacgtaca aataaaaagg ggcaaggtaa
    17281 atggcaaaat atcaagacat tgcagatgag ataaaacaaa agatccttca tgaggaatac
    17341 gaagtaaata caaccattcc ttcagaattc aagctgcaag aaatatatgc cgtcagcaga
    17401 catacgattc ggcaagcaat cgctgtgttg gtaaatgagg gctatctgcg taaagaaaaa
    17461 ggatcgggca catatgtaga tgacagctat aagaaagaaa aaacaccatt gaacagaaca
    17521 aaaacgatcg gtgtcatcac cacatatctc tctgattata tttttccgtc gattatccga
    17581 ggaatcgaaa acgaattacg agaatctggg tattcattgc ttttagccag tacgaataac
    17641 gatcacgaat tggagaaaga gtgtttgaag aaaatgattg cttatggagt agatggttta
    17701 atcgttgagc ctacgaaaag taatcagtat aacccaaact tagcacttta tgttgctttg
    17761 agagaaaaag gcattccgat ggtgatgatc aatgctgctt atgaagagtt aaatgcctct
    17821 tatatttgtt tagatgatgt gaaagtcggt tttatggcag cagaatattt gataaaaaaa
    17881 ggacatcaac gtctgttgtt catcacaaaa atcgacgatc ttcaaggaaa atacaggatg
    17941 aaaggtttta tcagcgcgtg tgaacagtat aatgtgcgat ttttgacaaa agatatcatt
    18001 actttcacca cggaaacacg agaaacaatt gctgaaaaaa cagtacaacg gttgaaagaa
    18061 gaacaagctt tagatggcat catttgctat aacgatcaaa tagctagttt attgctagat
    18121 aagttgacag aaaatggcta tcatattcca gatgacctat cgatcgtagg aacagacgat
    18181 tcttctttaa gccatttagg aaataaaaaa ttgacaacat tgactcatcc aaaagaaaag
    18241 ttgggacaag atgccgcacg gtggatcgtc acaacgattg aaaaaggaca ggtacagccg
    18301 aatattcttt atgagcctgt actagttgaa cgggaaacag taaaagaaaa cgaaaaataa
    18361 gagacgggtt tttaccgcct cttatttttt atatcaattt ggggctgtga caaaaatcat
    18421 caaacatgag aaactatttt ttgaacttgg tccttgtgac tcgaaactaa gcggttcctt
    18481 cttatttctt gaagctgacc acttctgtca cgaccacttt aagaatgatg gatagaaata
    18541 cgtttaggat aagttttttt agatgcactt tgttcgattt cctctaaaga tttcccacga
    18601 gtctccggta cgatctcttg aataaataaa actcctaaaa agcagatgat cccaaaaata
    18661 ctgaaaacga tttcttgaga catagaagca gtcatcacag gaaaaagcaa gctgactagg
    18721 aaagaaccaa tccaattaaa agaagaggct aggccagatg ctcgcccgcg gacagctaat
    18781 gggaatattt cgcctacgat tacccatgtc agtggagccc atgtgcaaga ataaaatgcg
    18841 acataaatac ataaaaacaa gagaattaac aacgaatttg tatgagcatc cagtacagtt
    18901 cctaaaacag ctggtaggat gaaagaaagt cccatcacgc taccgcctat tttcaataaa
    18961 gttcgccgat tgaacttgtc tgcaataacc aaaaagagca aagaaccagc aactaaaatg
    19021 attccttgga tgatcggcca catcaatgca tcgctcgctg catgcccagt cgctttttct
    19081 acgatcagcg gaatatagta aaaaatagca ttagctcctt gaaattgctg gaatactgct
    19141 acaccaatac cggcaataac taacgaacga tacttttcta aaaacaaagt atgccaagaa
    19201 gttacggttg tcttttcttg tttgacattc agctggattt gttcgaattc ctgttcagct
    19261 tcgtattctc tccttctaga atttcgagaa ggcggctgat cagattttgt aagcaatctc
    19321 attctataac ttatgtacgt acaaaacaag cgttttttat tatattcttg aaaataacca
    19381 cttgacatgt acgtacaaaa gtatacaata atcattgtaa gcgctactaa aaaagacggt
    19441 ttgatcaggt ttgacgagaa ggagggagtt aaatcattat gaaggaagta aatgcgcaaa
    19501 gtatgttaga tgtcatagat ggtcgtacat ctttaggaat tgagtttgga tcaactagaa
    19561 tcaaagctgt attgattgat tcatctttta ctcctattgc cgctggtagt tatgagtggg
    19621 aaaacaaact agaaaatggt atctggacct attcattaga agatatttgg aaaggactgc
    19681 gcgtcagtta ccgaaaaatg gcagcagaag tattcgaaat ctatggcgag aatcttacaa
    19741 cgatcgggtc gattgggttc agtgcaatga tgcacggtta tctagctttt gatagcaagg
    19801 aacagttact agtaccattt cgtacttggc ggaatacaat cactgaagaa gcagaacgaa
    19861 tattaacgga aaaattccaa tacaatattc cgcagagatg gagtatcgct catttgtatc
    19921 aagctatctt gaataaagaa gaacatgtga gagaaattaa ttttttgaca acgcttgcag
    19981 gatatgttca ttggcagctt actggtgaga aagtactagg cgtaggagat gcatcaggca
    20041 tgtttcctat cgatccttca acgaaaacct atcatcaaga aatgaaaaat atttttggtc
    20101 agttagtcga aaaagagcat gtagcgattg atttggatca cattttgcct aaagttctag
    20161 tagcaggaaa agaagctggc aagttaagcg aatcaggggc ttatctgatt gaccctacgg
    20221 gaaacctgaa gccaggaatc ccgatttgtc cgccagaagg agatgcagga accggtatgg
    20281 tcgctacgaa tagtgtagcc aaacgcacag ggaacatttc ggctggtact tccgccttcg
    20341 ctatgatcgt attagaaaaa gaactagaaa atgtccatcc ggaaatcgac atggtcacta
    20401 caccttcagg agaactagta ggcatggttc atacgaataa ctgctcttcg gatatcaatg
    20461 cttgggtcag tatttttgaa gaatttacca attctttggg tatggagata agcagagata
    20521 aattattcac ggtattattc aataaagcgc tagaaggcga tacggattgc gggggattac
    20581 tttcttatgg ttatttttcc ggtgaaaaca ttacaggagt aaatgaagga aggccattat
    20641 ttgtacgtac accaggtagc cgttttgatt tagcgaattt catgcgaatc catttagcca
    20701 gtgcttttgg cgcaatgaga atcggaatgg atatattgaa atctgaggat gtccaaattg
    20761 atcgtttagt ggggcatggg gggattttta aaacaccaga agctggacag cggattctag
    20821 catctgctat ggaggcacca gttacagtga tggacacagc cggagaaggt ggagcgtggg
    20881 ggatcgcgtt attagcagcc tatatgatag atcagcatgg gaaatcttta gaaaacttct
    20941 tggcagaaga cgtgtttgga caagaccaag gaatcaccat cacaccttca gcagaagaaa
    21001 tagcaggcta tcagatattt atgagtcgtt accgtgaggg gatcgacatt gaaaaaacag
    21061 cgattcatca attgcaagaa aaaggagtga tagaaaatgc tggaacaact gaaagaagaa
    21121 gtataccaag caaatcttga cttgcctaaa cacggacttg tcaaatatac atggggaaat
    21181 gtgagcgctt ttgatcctga aacacgatat ttcgttatca aacctagcgg ggtcagttat
    21241 gaagagatga cagcagacga catggtcgtt gtggacttgg acaatcatat cattgaaggt
    21301 aaactcaatc cctcttcgga tacgcctact catgctgttt tatatcgttc atttcctcaa
    21361 atcggaggaa tcgtccatac tcactcgaca tgggcgacga tctgggcaca agcaggattg
    21421 gatgttcctg cgatggggac gacgcatgca gatacttttt atggatcggt accttgtgct
    21481 cgatttttga cacaacagga aatcgacagc gggtacgaat atgaaacagg aaaagttatc
    21541 atagaaacat tcaaagaacg caaaattgat cccttagcaa ttccaggggt acttttacac
    21601 ggacacggtc catttacttg ggggaaagat gcgcaaagtg cagtaatgaa cgcagtcgta
    21661 ctagatgaag tatgcaagat gaatttattt accagacagc ttaattcatt ttcagaagag
    21721 ctgcctcaac ggattttaga taaacattat ttacgaaaac acggaaaaaa tgcgtattac
    21781 ggacaacaca aataaaagga gtggctaatt atgttgaata ttggagaaaa agaattttgg
    21841 tttgttgtag gatcacagca tttatatggg gaagaagcac tgcgagaagt aaagaaacat
    21901 gcacaagaaa tggttgatga attgaatgaa aacggccaat tgccttatcc tatccgcctt
    21961 caagaattag cagttaccgc tgacacgatc acaaaaatca tgaaagaggt caattatcga
    22021 gaagaagtag caggtgtgat cacttggatg catactttct caccagcaaa aatgtggatc
    22081 cgtgggacaa agcttctaca aaaaccattg cttcatttag ctacgcaata taacgaaagc
    22141 attccttgga aaacgatcga tatggatttc atgaatttaa atcaatccgc tcatggtgac
    22201 cgcgaatatg ggttcatcaa tgctcgatta aataaacaaa ataaaatcgt cgttggctac
    22261 tggaaacgac ctgagattca aaaagagatt gctgattgga tggatgttgc agtagcctat
    22321 aatgaaagtt ttggaataaa agtggctcgt tttggtgaca acatgagaaa cgtgggtgtc
    22381 acagaaggag acaaagtgga agcacagatt caatttggtt ggactgttga ttattttggt
    22441 atcggagatc ttgtacaagt aatcgatcgc gtatcagacg aagaagtcga acaattattt
    22501 gaagagtaca aggaattgta taccttcgac tatggtgatt ataaagaaaa gacatgggaa
    22561 gaacatgtaa aagtacaagc tcagcaggaa atcggtattc gccgtttctt agaagaagga
    22621 ggctataacg cttttacgac taattttgaa gacctttatg gaatgaaaca actgcctgga
    22681 ctagctgtac aacgtttgat ggcagaagga tacggttttg ctggggaagg cgactggaaa
    22741 actgcagcga ttgatcggtt gctgaaaatc atggctcgag gaaaagatac aggattcatg
    22801 gaagattata cttacgagct agctagtggc caagaagcaa tcttggaatc ccatatgatg
    22861 gaagtagatc cgacgcttgc tgcgacgaaa ccaagaatcg ttgtttctcc tttatcaatg
    22921 ggagatagag aagatccggc gcgtcttgta tttgatggga aagcaggaga aggggtagtc
    22981 gtatcgatgg cagacttcgg tacgcattat aaactgctga tcaatgaagt agaagccttt
    23041 gaacccacaa ctgaagcacc taatttgcct gttgcacgtg tgctatggaa aactaaaccg
    23101 aatttccatg aaggtgtcca ttcatggatt caggcaggtg gcggtcatca taccgttgtc
    23161 tcgctaaatc taacaacaga tcaaattgaa acatgggcaa aattagttga gttggaaacc
    23221 gttgttatta gataaaaaga ggttgtgaaa aagccgctta gttttaagaa aaaagaagag
    23281 attgacgaaa tttgcttttc aacttttttg aaattttgtc ttattatcaa tgttagctaa
    23341 ctgaacacca tttatcaaga aatctcaccc acaatatttt gctctcaaaa ataaggctta
    23401 tggcagtaac ttgccatggc cttattttta tcgctaagcc tactctgctc tagctgctat
    23461 ttttgcttct aggaagtcaa atggtaaaca gttgtctaat tgcagtaatt tcgagatact
    23521 tgttagtaag gagggggttt tatgtatcac gttcatttga ttgatgctat tccagaacaa
    23581 gagatagaaa agttacaagg ggaatatttc aaaataatag atgatttgcc agccgaagga
    23641 atagtcattc gtagtacgaa agtgaaagat gaatggctta caccagattt gctagcaatt
    23701 tcaagagctg gtgttggggt gaacacgatc aacgttgaaa aagcttctga aaacgggacg
    23761 atcgtcatga atacaccagg cgtaaatgct aatgcagtaa aagagcttgt tttgtgctgt
    23821 ttgctcttat cttcccgtcc gataatcgaa gcaagtcgta tggtacaaac actaacaggt
    23881 cctaatattt tagaacaggc agaaaacaaa agaagtactt atgtgggacg agaattgcaa
    23941 ggcaaaacca tcggtcttct tggtcttggc gcgataggca caaaagtagc gctaagttgc
    24001 tatggcttag ggatggatgt cttgggttat tctatccgcg atgcccagtt ggattatgtt
    24061 cggcaagcag atttagaaac agttctttct acctcggatt acatcgtagt catgctgccg
    24121 ttgacagaag atacaaaagg attgatcaat caggcgaata tagaaaaaat gaaaaaagat
    24181 gctgtactta tcaatgtagg aagaagtgaa atcgttgata aatatgctgt tatgcaagca
    24241 ttggaaaaac aacatctggc caaatacgtg actgatttcc cagaagaaga gttcttagaa
    24301 aatgaccgta tacttatgtt gcctcatcta gggggatcga ctcaggaagc tttggcagac
    24361 agcggacgtt tggcagttga agccttaaaa gattatcttt tgttcggtac ggttagagaa
    24421 gcggtcaatt atccatccgc acgaatgttt ttccaagcgc cgtttcgatt cactatattt
    24481 tatcaaaaga aaaccaatat attagcagag atattttctc tgctgaataa gaacgagcta
    24541 gtgatcgcag atatatcgcg taatcataag aatgagcatg tctacatatt gattgacata
    24601 gacagtactg attttgaaca gctcaatcaa gtgaaacaac agctagaaaa aatttctgga
    24661 gtacgaaaag tccgcttatt aaagaaacca gaaaggtcat aaagaaagag aatatttgga
    24721 tttcattaat tcttcttttc gttctttact ttcgaatttc gtacaactaa aataagagaa
    24781 acaacattct tttgttgata gaaatgaagg agcaagcatt tgaaaagata tataagcaga
    24841 ctttcaacca aacagaaaaa gattttaaaa ggaacagtag cgatcatcag tatctttttt
    24901 cttgttgttt ttagtaattt atttttgcag tggtgtcaaa atgatctatc cgtagacctt
    24961 gcgttgaaat ttgctttttc ttggcatacg gaaaaattct tcttagcctg tttcgtcttg
    25021 ctgattattc ttatttttct gattgcacta gcaggatctg tccctttagg ttcccttacg
    25081 tatgtcgtag ccatcggtgt attaggtttt gctaattata tgaaaatgag ttatcggcaa
    25141 gaaccgattt atccagacga tttgaagatg atcactgaaa tcggattgct gaaagatatg
    25201 accggcacta tgctgtttac agtgatctta gctgccgcag gaaccgtgct aggattgttc
    25261 tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg
    25321 acactgcttg tggcaattgg actgctaggg tatatcagta atttcaacaa tcccgataat
    25381 ttgttgcgaa aagcatataa taaaacggca ctatggatac cttacagcca gaaaatgaac
    25441 tactataaca ctggttttat cggtgggttt ttgtacaatc tgaaggtaga accaatggat
    25501 gaacctgaag gatattcgaa agcaaagatc aaagaaatca ctgaaaaata ccagaagata
    25561 gccgatgaaa aaaataaagc tgtagaggaa gagtcgccga acatcgtgtt cgtaatgagt
    25621 gaaagtttct cagatcctag tcgattaaac ggcgtagaag tatctggtga gccattggca
    25681 gattattatg aagtagctga tcaaacatat agtggcaata tgctttctca aaactatggc
    25741 ggtggaacag caaatatcga attcgaagca ttgactggtt tttctatggc gttgtttaat
    25801 gcgcaactca ccactcctta cacgatgcta gtgccaaaaa tggatcagct tccttctatc
    25861 gtttcagcgt taaacgcaca atcataccag acgacagcga tccatccata caatacttcg
    25921 atgtataaaa gagaggatgt ttatcagata ttaggatttg accagttcat cagcgagcgt
    25981 acgatgacct acacggatac gatagagaat aatccatata tttctgatga gtcagcttac
    26041 aaagaagtaa tgacacttct caaggaagaa aaaacacctc agttcattca tttagtcacg
    26101 atgcagacac atatgcctta caatggaaag tatgatagac tgggctattc tgcagagatt
    26161 tcagatggaa gtgggacgct tgacttagag aattatctgc aagatatttc ctatagcagc
    26221 acggcactga agcaattcac ggaagaactg aagaatcttt ctagaaggac tctagttgtt
    26281 ttttgggggg atcatctgcc agggatctat tcagatacca tccaagccaa aaatgataaa
    26341 caaacattgc atgaaacaca attcttgatg tttgacagta aaggaaaatt agaaaagaaa
    26401 acgactcaag atgcaataac cagtcctttt tattttgcag cgaatctaat ggaacagacg
    26461 aatcagacaa cgaatggttt ttatcagctg cttttatcat tagaacaaga acttccggcg
    26521 tttgaacgag aactgtacta tcaaaacgga cagtggtata aagaagcgca attcaatcgc
    26581 tcgcaacaag agatttacga cgaatatcaa ttgatccaat atgacattgt cgcagggaaa
    26641 caatatagtt tagcagatgg tttctttgag cacgaataag aatggcgagc tgtatagaaa
    26701 ccattaaatt ttttcaagta cttgtcattt gaggataaaa acaagtagaa taaataataa
    26761 caacaatgga gaaagtagga aaacaaatat gttaccgaat attggctatt atgtgcagtc
    26821 catcgatcaa atcgtcaagg aaaccgaagc aatcggagaa ggaatgcatc cgaaatatga
    26881 agtgatcaga aacgcaatcg atgaaggaaa aacaagtgaa ctggacagat cgactcttga
    26941 agagaccgtc cagttatttg aagaaggaac aaaaaaatat cgggcaatgc ttaagaaagt
    27001 cagtacattg cgcccaccag caaaagcaat gggcattcat aaaaattttg aaaaagctta
    27061 tctttcatat gtagcaggct gtgaagaaat gattcaaagt attgatcttg agtcaggggt
    27121 agataaagat ttattcaata catctgaagc aaaataagac caagcgactg accaattgac
    27181 agctgccatc caaaaaatga ctaatttgtt attgaaaaaa taaaagaaaa atttgatgac
    27241 aataaaaact agagttatct aatataaatg taatattgtc tttatgatag agtgaacaaa
    27301 atcctttatg ataagaacat acctataacc aacaaccttg tacttactca aaattgagta
    27361 aatactcctt ttccgcagag tctaaccaac tcggcggttt ttttatttat agaaaaggac
    27421 atcagaacag acgaaaaagt cttgcgctga tgtccttttc agatagtatg gaaggttgat
    27481 tagttttcta cattccgtac atattgttca aagtagttct gtaaatcggc tttgagtttc
    27541 tgatattcct cttcgctata ctctttatct tcgattttcc catcaaagaa aggaatattc
    27601 agttcttccc gcaacctgtc taatacgtct tgctgattca ttttattctc cttttcttgc
    27661 taacgatcaa aataaacaca gacacattct ggtgcataga catctttttg caataattcc
    27721 agtaatcctg tttcaggatg acggcggtat aatgtcagat tatcggtatt ctgattagca
    27781 catacgacaa aaccattgct tggatctagt gcgaaatcgc gagggaaatc gccttctgtt
    27841 gagatgattt gtttattttg gatgtgagaa ccatcttcgg aaactgcgaa tacggcaatc
    27901 gagttgtgtc cccgattaga agcgtaaagg aacttgccat cattggaaat gcgaatagca
    27961 gcccctccat tttcaccgtc gaattcttct ggcaaggtcg atattttctg taattcttca
    28021 aaatcacctg tttctttatc ataagaaagc actcggactg tgctgtctaa ttcaccaaat
    28081 aaataggcga attgtccatt cggatgaaag acgagatggc gagggcctgt tcctggctct
    28141 gcagtgaagg tagttacaag attcaatttg ccgttttcgg acacatcata agtatacaca
    28201 cgatctgtac caagatcaca cacgactaat cgttggtcag gagtcagatc cgtataatgg
    28261 acatgcgcat gatcttggtt tttatgaggg cctgtcgctt cttgatggaa tactgaatcc
    28321 gctgctttca gctcgccatc tcctaatagg tgatagacat ttacttcgcc tttatggtag
    28381 ttggccccat agagcaattg ccgtgtttca tcaaccgcta cataacataa aggagcccca
    28441 gactctgtta ccgcattcaa aaaatgaaaa tccttgtcgt acgcaccggc accacctaat
    28501 ccgtctacac tggtaaccgt gtaaagattg ccagctttgc tttttgctag gtaagtaggg
    28561 ctgttctctt caacagccaa tgaaagactg gataattctt ctttttctgt atcaagatcg
    28621 atcgtataga ttcctttgct ttcgcggcgt gtataggttc ctaaaattat cttttcgatc
    28681 atatgagctg attcctccta atggtaatga aatagttttc gtttttattc tatcacataa
    28741 ttattcccca cggctaaaga atgagaatgt gttatactgt ttttgaaagc aagtaaagga
    28801 gagagtgcga tgattcaggc aacgattaca gaaattggaa cagaagcaat aaacaaggaa
    28861 gaaccgatgc tgattttgtt cgataaaaca gcaacagctg ccttgcgaaa atattcggtg
    28921 attcaagagg tgtcgaaaga cgaacctttt tctttgaaag caggcggaac gattactttt
    28981 gatgatcagt cgtatacgat cgattatgtc ggtccgatgg ccaatggaaa tttagagacg
    29041 gtagctcacg tatcattgat ttttgatacc tgtccagaag aaggacaaat tgcaaacgga
    29101 atctatctcc atccgcatga actgcctgct attggtttgg gaagccaaat tatttataac
    29161 taacaggagg acttatggaa gggaaaaata agaagaacag accggtgtct tggttttggc
    29221 gctggttttt aaataatcaa gtggtcacag ctttacttgt tgtattgtta atattgctga
    29281 tcgtcttttt atttacaaaa gtttcttatt tgtttgcacc gatctggcaa tttttagcaa
    29341 tcgtaggact acctattatt ctggcaggca tcttgtatta cttgatgaat ccggtagttg
    29401 attactttga gaagcgaaaa gtaccaaggc tctatagtat cattgggctg tttgttttgg
    29461 tcgttgcatt aatcgtatgg ggaagcgtag tgatcatacc taagatccag gaacaaacag
    29521 tcagctttat cagtaacttt cctcaatacg ttgatacgat cgataacaaa ttgaccgaga
    29581 tcttgagaga cccgctgttc aaccaatttc gtgaacagtt agaaacagca ggtgataagt
    29641 tcatgaattc agcaggtgat atgatccaag acatatctaa gagtacagtt cagagtcttg
    29701 ggagttttgt tggtgcggta gcgactattt tagtagcttt gctgacgatg ccgtttatct
    29761 tattttattt attgaaagat ggtaagcgat tagcccctta tttcgtcaag tttttaccaa
    29821 cgcgcatgca aaaaccgacg ttgaatgtgc tgagtgaaat gaatagtcaa gtttcttcat
    29881 atataagagg gcaactaact gtggcttttg ctgtggcaat catgtttatg attggttttg
    29941 caatcattgg tctagaatat gctgtcacgt tggggatcat tgcagggttc ttaaatctga
    30001 tcccatactt aggttcattt ttagcaatga ttccagccat ctttttagct attgtggcag
    30061 gtccattcat gatcatcaag gtactaatcg tttttgcagt agaacagaca atagaaggac
    30121 gcttgatctc tccgttagta ttgggaaatc agttagcaat ccatccagta acgatcttat
    30181 tagttctgct gacgtcaggg aaattgtttg ggatcgttgg tgtgatctta gggattcctg
    30241 tttatgcagc agctaaagta gtgattactc atgtttttga atggtacaca acgatttctt
    30301 ctctttatga agaaaatccg ttgaaaaagc agaaagaata acaaaaagag acagaggaaa
    30361 atttccaaag cacaaatgtt gctttgaaga tttttccctc tgtctctttt atatacattt
    30421 acctcaattt taaaatgatt tacctaaagc agcagcttta tctaatgcat tttgcatcaa
    30481 ctcatctgca cggtcagggt cataatcgat tccttcgatg aatagttgat ctacttgatt
    30541 gatgcctaca aagttcaaaa taccttttac atattgagag gcaaagtctt gtccgttgta
    30601 tacaccgccg ttagattgga tgtgcagcgc ttttttacct gtcactaatc cttctggccc
    30661 gttttctgta tagcggaacg tttttccagc tacattgaca gtatcgaacc aagcttttaa
    30721 ttttgttggg atatttagat tccataaggc gtttgcgata acgattttat cagcagcaag
    30781 gaactgctct gtcaattcat tgaaacgagc gattttttgt tgttgacttt ctgataagga
    30841 agtgaattct gcaccagcac gtaatgcttc ccaacctgat aataattctt catcgatttc
    30901 tggaatatca tcgcgataaa gttccaaaac agtcacttca tcatctggat gagtttcttt
    30961 ataacttgtt aaaaaagacg tcaacgcctt aactgtacgt gattcttctg cagttagggg
    31021 atgacctttg actactaaaa cagatgccat atgcttacct tctcctttta ttgctatgat
    31081 tgttataact atgactaaca aaatcagttt actcgaaaaa gaaaaggcta tcaagtcata
    31141 ttgataaggt tgaagaataa ataactaaaa aagacaggat ataaaagagg ttgtgacacg
    31201 atttttgtca cagcctcttt tgaatctaac aagcaatgtt caagcaacgc ttgactgaag
    31261 agcttccatc ccaccaatga tataaacagc acgaaatccg ctagcagtaa gttgatcggc
    31321 taatgcttct gatcgtctgc ctgtatagct tagaatataa taaagttttt ttggatctag
    31381 attttttaat tgtcttggca agatagcagc ggggatagaa agcgagtggc ttaagtgttt
    31441 ttttgtgtaa tctaaagtat ttcttgtatc taaaacgatg ctgtcagcat cgagcggcaa
    31501 tttattgaag tcttggacac taattgattg ggccatttat ctcatccttt gtttagtatg
    31561 tggctatttt aatgggcagc tctcattaat tcaaatggaa ggattcctct tatcactttt
    31621 ttaatgtatc ggataataat gaaaaaaaac tatgcaaatg tcttgcgaaa caggtgggag
    31681 gtgttataat gacatagcgt catatgacaa tgtgtcatct tagaagaaag gaggatccat
    31741 atgccgacac agacattttt ccatttgccg aaagaaaaac aaaaaaggtt aattgaagcg
    31801 gcgagaatcg agttttctag agtacctttg aaagaagcct cgattgctaa tatcgtcaaa
    31861 ttagcggata tccctagagg cagtttctat cagtattttg aagataaaga ggatctttat
    31921 ttttattact ttgagacaat gagacgtgac agctctaaag atatgttgca actaatgaaa
    31981 gaatccgatg gtgatctatt caaaggattc gaatcctatt tttctaaaat gatttatgag
    32041 attttggaag gtgagaatgc ttctttttat cgtaacttgt ttatgaacat ggattaccgt
    32101 gcctcaagaa aagtcgctcc tcatacgagt cgccaaaaat cgcctcacga tccacataaa
    32161 gaacacaaac aagatgcaaa agagttgctt gaattgattg atcggacgaa gctgaaagta
    32221 aaaaatgatc atgaattgga attactgatc caattagtga tgaatactgt atttacaaca
    32281 attgcgcatt cgtatcgctt attttcaaca tctgaagatt actcagtaga gcaagcaatc
    32341 agtgaattca atttgaaact ttcttggctg aaaaacggag tatataaata gaaagaaggg
    32401 aactagatgc taaaaatcgt aaaacggatc tccttgactt cagcaattgc agctgcagtt
    32461 ttcatggtga tccaagtact ggcagattta tatttgccga cacttacctc caatatcatt
    32521 gataaagggg tagcgcaagg agatgtcgat tatatttggc atacaggttt tgtgatgatc
    32581 ggtttttctt tgatcagtat cttagctgct attgcaaata cattttttgc tactcgagaa
    32641 tcacagaaac tgggtaagca attgcgtacg gatgtctata aaaaatcaga gagcctgaca
    32701 aaagatgcgt ttgacaaata tggaacggcc tctttgatca caagaacaac caatgatgtg
    32761 actcaaatcc aaatggtcac acaaatgttt ttacgtatga tgatcaatgc accaatcacg
    32821 ttgattggcg caagcatact tgcttatcaa aaagatcatc agttgacaaa aatcttctta
    32881 gtcgtcatcc cagtaatgat tattttgatt ggtggaatca tgtattttgc tgttccatta
    32941 ttcaaaagca tgcaaaagaa aactgaccgt ttgaacttgg tatttcgaga agggttgaca
    33001 ggtgtcagag tcatccgcgc attcgataag acacgctttg aagaaaatcg ttttgattta
    33061 gcgaataaag attatacaaa tacagcaatc aaagttaata cgatcgtcgc attgatgatg
    33121 ccgatgatga cattgatcat gagcggtaca aacgtagcaa tcacttggtt tggtggacac
    33181 tacatagcag atatgacctt ggaagtcggt aatctgattg catttatgac ttatgcgatg
    33241 cagatcttga ttagctttat gatgctttcc gctattttta tcatggtacc tcgtgcacaa
    33301 gcttcagctg atcgtatcaa tgaggtgttg gatgaaaaaa tcggtatcca tgaccctgaa
    33361 aatcctaaaa ccgtttcttt tgctggaaaa gaagcaacat tggccttcaa tcatgtcaat
    33421 tatcgatatc atggagcaga aaaacttgca ttggaagata ttgattttca agctaagtca
    33481 ggcgaaatcg tcgcaatcat aggaggaact ggttcaggta aaacgacctt agttaatttg
    33541 atcccccgat tatatgacat cgaatctggt tcgatccaaa taaacggaac ggacatcagt
    33601 gaaatgactc aatataattt gcgggagcta atgggctttg ttcctcaaaa agcagttctt
    33661 ttctctggaa cgatccgaga caatatgcag tatggtaagc cggatgcaac agatgaaatg
    33721 atttggaaag cgttagaaat cgcacaagct aaagattttg tttcagagat ggaagatgga
    33781 ttagatagcc atgtggaaca aggaggagga aacttctccg gcggacaaag acagcgttta
    33841 gcaattgcac gggcattagt cgaaacagca gacatttatg tgtttgatga ttccttctca
    33901 gcattggatt tcaaaacgga tgccaatctt cgccaagcat tgaaaacgaa tatgaaagaa
    33961 agtatcacgg ttttagttgc tcaacgagta agtacagtaa tggatgcaga tatgatcctt
    34021 gtgctagatg aaggaaaact agttggcaag ggaacacatg aagaactatt ggcaacaaat
    34081 gaaacttatc aagaaatcgt tcattcacaa ctaagagagg aggacttagc atgagtaaaa
    34141 caaacacacg tccctctcac ggaccagcag gcggtccagg acacggcgga cctggcagga
    34201 atctcggtgt aaaaggggaa aagcctaaaa atttctgggg aacagtacgc cgattgtttg
    34261 gctatatgtc taaacgtatg gttgcaatca tcgctgtatt tgtactagca atagcagccg
    34321 tgattttcca aatccaaaca ccaaaagttt tggggaaagc aactacagaa atattcaaag
    34381 gtgtaatggc tggcgcgcaa cagatgcaac aagggcaaca agtaacaaaa tttccgatag
    34441 attttgataa aattgcgcaa atcattgcaa cagtaatcgt gatgtatttg atctcagctg
    34501 tattcaactt tttacagcag tttattatga cacgtgtttc acagcggaca gtttatgagt
    34561 tgcgtcatga cttggaagcc aaaatgaatc gtgtaccgat ttcctattat gatacacata
    34621 caaatggcga tatcatgtct cgagcaatca atgatatgga taacatcgca agcacgctgc
    34681 aacaaaattt gactcaattt gtaactagtg cagtcacatt gatcggggtc atcgtgatga
    34741 tgctttcaat cagttggcaa ttaacgttag tcgcgttatt gatgatccct ttaagtttga
    34801 tcatcgttat ggtcgttgcg ccaagatcac aagtgttttt cgcagatcag caaaaaagtt
    34861 taggtttatt gaataaccaa gtagaagaaa catatggtgg tcatacgatc gttaaaacat
    34921 tcaatcatac tgaaaaagat caggaagtat ttgaaaaaga aaatcagaat ctgtatgccg
    34981 ctggttggaa agctcaattt atttcggcaa tcatcatgcc gttgatgaat ttcgttaaaa
    35041 atctaggcta tgtgttcgtc gctgtactag gcggagtaaa agttgcaaat gggaacatga
    35101 cattagggga tgttcaagca ttcttgcaat atacgacaca gttctctcaa ccaatcactc
    35161 aacttgcaag tttgatgaac actatccaat caacggttgc ttcagccgaa cgggtatttg
    35221 aagtgttgga tgaagaagag atgtccaatg aaaaatctgg ccttccagaa gaaaaagata
    35281 caccatacaa agttcgtttt gaaaatgttc aattcggcta cacaccagat aacttgctaa
    35341 tgactgattt caacttggat gtcaaacctg gtgagatggt cgctattgta gggccaaccg
    35401 gtgcaggaaa gacgacactg atcaaccttc ttgaacggtt ctacgatgtt tctggaggca
    35461 gtattcgtta cgatggtgta gatacccgtg acctttcacg tgatgaattg cgcgcacagt
    35521 tttcaatggt actgcaagat acgtggctgt ttacaggaag tatctatgac aatatcaaat
    35581 atgggaacga cgatgcaaca gatgaacaag tcattgaagc tgctaaagcc gctcacgtag
    35641 atgattttgt tcgtaaattg ccagaaggat ataacactgt attaaacgag gatgcaagca
    35701 atatttcgca agggcaacgt cagctgatca ctattgcgcg ggcattctta gcaaatccgg
    35761 atgtcctgat ccttgatgaa gcaacatcta gtgtagatac acgaacagaa atactgattc
    35821 aaaaagcgat gaaccgccta ttggaaaacc gaacaagttt tgttgtcgct caccgcttgt
    35881 ctacaatacg cgatgcagac aacattattg tgatgaatca tggttcgatc gtagaaactg
    35941 gaaaccacga tacattgatg gaaaaagacg gattctatgc agatctttat aacagtcagt
    36001 tttctgaaga agaagcttca tagaaacgta caaatttatt atttttcttt accgcatgaa
    36061 gcacatttca agaaaatgag tttcatgtgg ttttttactc atttatttac aaaaaacaag
    36121 taaaatgcga agtttctcac acttttttta acaaaaacaa agttattgct tgaaagaaga
    36181 agaatccatc gttaaaataa agcttgacgt agaggggagc aagaaatatg agagattttc
    36241 aattgggggc tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa
    36301 tgattgattt ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag
    36361 ccattttagg aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag
    36421 cgaatgatca catggggaag atcaaaaagc tgaaccgttt ctcattgatt atccctagca
    36481 cagaagaact ggcagatatt ctttgccgta ttcgtaaaat gaattatcca atcaaagaag
    36541 ctttagaaga tcatggacgt atggggattt tactcgtaga cccagaagag aatgaattag
    36601 aaatttacca tgaagagaat aagaatgacc aaagagaagt tccagagacg atggatcaag
    36661 aagcgttgtt aagaaaatct gaagaaaaat tcagtaaact atcagcagga tcttattttg
    36721 aaaaaatcca tttaaatgtt actgatttag caaaagaacg ccaatttctt aatcgtgtcc
    36781 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagacttcc
    36841 atgttggatt gacagaagca agtggtggtg ccatcgattt gcctacagat gaagtgattg
    36901 gacttgattt tctgaaattc atgattgata ctgaaagcat ggaaggactg attgctcact
    36961 tgcagcaatt atcagtcgat ttcttcttgg acaaaaagca tacagtactc acaatctatg
    37021 atgccatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
    37081 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
    37141 aacggtcata ctatcacttg ttctagagat agaaagagca aacagtatac cgaatgaaac
    37201 tgcgtggttt tccgcgcagt ttttttgtgt aaaaaggttg attcctcata gattaagctt
    37261 taagcttgga ctgtaagaat ctgacagaaa agcaggagaa aaagatgtac ccacaaacga
    37321 aacagcttat tttgaaaaaa atggaagaag gattttatcc aggtgtagca gccgccttca
    37381 tcaaagaaaa tcaagtagaa tcatttacag ctggctttgc ccaagtcgtt cctgaaaaac
    37441 agccgatgac agaagatctg ttgtttgatg cagcatcact gacgaaagtc gttgcaacca
    37501 cttcattagt tttgcgtcta ttagaagatg gaaaaataga actcgatcaa ccactgcata
    37561 aatatctttc tgaattcgaa aatcagacaa tcactttgcg tcatttactg acacatactt
    37621 ctgatatcaa aacatggata ccaaacagag atgagctatc tcaggaagaa ttgattgccg
    37681 cttatttgaa gctagaacca ggtgagcaac taggaaaaga agtaaaatat acagatgccg
    37741 gaacgattct tcttggattg atgctggaaa aaatttacca agaaactgca gtggaaatct
    37801 ttcaagaaca agtattggaa ccattagaaa tgatgaacag cttatttctt ccttttcctt
    37861 ccaaaaaaat cgttcccaca gaacaactgc cttcgggtga ggttttgcgt ggacaaactc
    37921 atgatccgaa agcaagagtt ttagctgaac atgcaggaaa tgctgggctc tttatcgatt
    37981 tgaacgacag cattactttt gtccaaatgt atttgaacaa gggaaaaatc agtaatggtc
    38041 ggtttttaga agaagaaacg attgattctt tgctacaaga tcaaactcca gaaagaaatg
    38101 gctatcgttc tctagggtgg gatttaaaaa aagatgtagg agacggtcat gcgattcttt
    38161 ttcatactgg ttatacaggt acgtttttct tgattgatat aaaaacacaa gaagcgttga
    38221 tatttttgtc taatcgcgta catcctgtcg atcatcgtgc ggagtatctc atatgtcgag
    38281 atgaaattgt tgaaaatttt ttgaaagaga aagcggtatt acagaaataa tgctataata
    38341 gctatgaaaa atgagaggag tttgaattat gcaagaacct atgtttttaa aaccagtttt
    38401 tcaagaaaaa atttggggag gaagccgctt acgttcagtc ttcggttttg atatccctaa
    38461 cgataaaatc ggagaagatt gggcgatcag tgcgcatcct catggtgtca gtgtagtaga
    38521 aaatggcgaa ttcaaaggga aaagattaga tgaactatgg tcagaacaca aggaattgtt
    38581 tggccatcct tcagaacaag tgtttccatt attgatcaag atcttagatg cagaagatga
    38641 tctatctgta caagtacatc cagatgatgc ctatgggatg aaacatgaag gtgaattagg
    38701 aaaaactgag tgctggtata tcatcgatgc tgaacctggt gcagaaatca tctacggtca
    38761 tcatgcaaag acaagagaag aactagctga gatgatcaaa aatgggcgct gggatgatct
    38821 tttgaagaaa gtaccagtta aaaaaggtga tttcttttat gtaccaagcg gaacgatcca
    38881 tgctattggt aaagggatca tgattttaga aacacaacaa agctccgata cgacctatcg
    38941 cgtgtatgac tacgacagaa aagatgcgca aggacaaaca agagagctgc atatccaaca
    39001 atcgatcgat gtaacaactg tccctgccaa aacaccagaa ctacaaatca aagaagttcg
    39061 aaaagggaat tcatcgatcg tcacttattt agaaactgag tttttcaatg tatatgagtg
    39121 ggatattaaa ggaatcgcat ctttcaaaaa acaagcccct tatacgttga tgacagtgat
    39181 tgaaggagca ggagaccttg ttgttgacgg aaaaacatat ccattagaaa aaggaacaag
    39241 ctgtatcatt ccaagtggcg tttctgagtg gacggtccaa ggtgaactag caatcattgc
    39301 gtctgaacca ggcaaatagt tcccagatag ctgaaagcgg cagacggttt tcgtttgctg
    39361 ctttttctac ataaaatccg tctatcaata aatagaagta tggtgaaaaa gatggaatac
    39421 aaatggaaag caaacagcaa cagtcgagtg tttacgaaac aactgagaaa agccggacac
    39481 cacttgaatg agatgatcat gccaaaccta agtctgttta ttgcttgggg aatcttgtca
    39541 ttagtaatga aaggtgtgtc tggcgatttg cacgccacgt tggaagaggt atgccactgg
    39601 atgattcaac tgcttttacc aatcttgatc agctattcag gcggaaaatc ggtaggggga
    39661 caaaaaggag cagtcgcggg cgcaatcgct tctttagggt tgattgctcg ttccgatgcc
    39721 ccgcaaatcg taggagcgat gattataggg ccgcttgccg gcatctcgta tgaacaattt
    39781 gtttctagat ttcatgcgaa attcaaaggt ggttatgaaa tgcttgtcag caacttacta
    39841 gtagggctga ttggtagtat tatttgtata attggaatcg tttttattga gccagcttta
    39901 aatagtttcc acctgttgct tgtctccgtc gtgtcttggc tagtcaaaat gaatctgttg
    39961 ccgcttgtca gtttgatcct agaacctcta aaagtactat tttttaataa tgcaatcaac
    40021 catggtgtgt tgacaccttt aggaatcgaa ttctcacaaa cagtcgggta ctcttacctg
    40081 tttttgatgg aggcaaatcc gggacctggt ttagggatct tacttgctat cctttgtttt
    40141 gcagaaaaac aggaaaaagt aaatgcgagt ggagcattga tgatccatgc gattggcggg
    40201 attcacgaaa tctattttcc atttgtcttg ctgcgaccgt atctattttt agctgtaatg
    40261 gttggtggag caagtggcac gttgatattc caatggttag atgcgggatt agctactcct
    40321 gtatcgcccg ggtcactact cgtgattttt gccaatacgc cttatccgca actagctggg
    40381 gtaagtatag ggatagcaat cagtacattt gtgaccttca tttgtgcaac gctgctattg
    40441 aaattaccga aaaaagaaga aggagacaac acggtcaaag aaaggggaga aattttggaa
    40501 ctatctacca ttcattctgt cgtttttgcg tgtgactcag gcatgggatc aagcgctatg
    40561 ggagctgccc tgctgaagaa aaaattagca gcggaaaaaa ttgatctcgc tgttggatac
    40621 agctctgtct atgaattgaa caatcaagta aatcagcttg ttatcgtcca acatgaatta
    40681 gcggatcttg caagagaaaa agtacctcaa agtcaaattt tcgtattgaa tcactttttg
    40741 gaaacagata tcttgctgtc acgaataaaa aaagcaatcc acgaaccaaa ttggaaagaa
    40801 aaaaaagaga gagtagaagt aatagaacag aaaaagtcgc ccgtcaacgt tgtgtttttg
    40861 tataaaaata atagacgtgg ttcacaaacc atgggcatgg cgattttaag tcaattagca
    40921 aaaaaagaga aaaaacagtt tgttatctta aaggaaccaa tcgaacagct caaaatccaa
    40981 gaagaaacga tttatatcgc ccaaagagaa ctagctgtaa atgaacagct aaaagaaaag
    41041 atccctcagc tagtcctagt ggaacattgg gtagctacga atgaatacga aaaacttgtg
    41101 aagggagaaa aataagatgt acttatcgcc cagagaaaaa caattattag cagaatttct
    41161 cagcagtccc tcacctgtga ctatacaaaa aatgatgaat ttattgaaag tcagcaagcg
    41221 gaccatttac cgcgaattag ataatcttga agtctcattg aaatcggtaa atgcttcgtt
    41281 gaaaaaagta gcaagaggtt cgtttgtcat tgaggcatcg gaagaagtaa tggagcaatt
    41341 aaaagaaaat acagagggga aaagcgatga tttgcctact gctaaacgcc aacacgcaat
    41401 tttagccgag ttgttactaa cgacagaacc gttgagtctt gctcatttta tggaattata
    41461 ccgcatcagt aatactactt tttatgcaga tatcaaacag ttggaagaaa gtatccgcca
    41521 actgcctttg gaaatcattc ggaaccaagg atatgagatc gtaggaccgg aaaaataccg
    41581 tcggcttcta acagcgaatg tattagaatt agaaatcaat gaatatgaac tttttcatag
    41641 tattcctttt gattcatcgc tgaattactt ttttcgattc gttgatccgc aacatctgtc
    41701 tcttgcacgc caagtagtcg gggaagaatt gatccaaaaa aagactaatc taagtgatag
    41761 aaaacttgaa catttggttt tgatgctgac attgacgatg gatcgtgtaa cgaaaaatca
    41821 tttactgaca aatgagacgt ataatggtct ggcaaacaaa gaattgcttc agaccgctaa
    41881 acgattattc tcacggattg cttctgagac aaaacaactt tacccagtaa atgagatcgt
    41941 cttttttgca ggattattaa atgacttcac gaactcattt gatcagaatt tctttgaaga
    42001 aacgttcgat acacagcttg cttatttagt aaaacaactg attcaagaag taagtgaaga
    42061 gacgaatgtc catttttatg aagatgaaac cttgtacaaa atgcttttga cccatttgtc
    42121 tggtgtgttt tcaagagccg ttttacaaga agagacactg actaatccga ttttagaaaa
    42181 aatcatggtt caatatgagg aaattgccaa tgcaatccgc aaagcagtag agcggatttt
    42241 tcaagaaaag aaattatcag aagaagagat cgcatatatg gtccttcatt ttgcaaattc
    42301 attggaaaga agcccgaaag aaagcgatat cagtatcgca ggattctcac caagcggctt
    42361 agcttctact agcatgttgg aactgcgttt gaaacggtat tttcctttca ttcaacagat
    42421 tcatttctat cggatcgttg atttgaaaaa aattgattta caagaagaat atgatattgt
    42481 tatttctaca tctattctac caggatatga tggaaaatat attttagtct ccccgctact
    42541 gctagaagat gaagtcaagc agctaaaaga agctttttcg catattgaga agaaaaaaag
    42601 acaaaagaat acgtataaca gcaataacga gtggatgcta gaagatactt atgaggaaac
    42661 gatgcatttc atggagcaaa tcaatcaatt acttgatcgt ttctttattc aagaactaga
    42721 taatcctaat acggtctctg aaacgattcg tagtttattt ccttattttc ctgaaggact
    42781 agtaacagat gaagaggtgg ttcatcgccg actgcttaag cgatatgaac aagcacctat
    42841 cggaattcct catacaaata tggggttatt ccatacatct agctcattgg tttctaaacc
    42901 gatcttttgt attttcaatt tgaaacaacc gttgacaatt gacgggatgg atagacagcc
    42961 agtcgatttg tcccgtatgt tagttatgct ggcgccttcg ccaatccaag aaaaagacgg
    43021 gattctttta ggaaagatca gtggagcaat catcatgaac gatttgaaca ccgagatctt
    43081 ccgctcagga aataaagaaa tcgtttatca attgttagcg aaaatcctga ttgaagaaat
    43141 aaagaaatga gggtgaaaag atgatccagc taccagcaga aaaaatattg ctcgatcaat
    43201 cctatcaaaa ttggaaagaa gcgttgaccg agatcggcca gtggatgacc caacaagggg
    43261 gaatcaaacc ggcatatatc caatcaatga tcgaaagaca agaaaaagcg agtgtctata
    43321 tcgggaattt cgtagccttg ccacatggga ctgacaaaga tgaagaagta gtagaagaag
    43381 ggatctattt agtccaagta cctgatggtg tggactttgg aacaaaagag gaaccaaaaa
    43441 tcgcgacact cttatttgta gtggctttaa aaaaagaaca tcagttagct gtgctacaag
    43501 atttagcctt tttttgttca gacatcgacc aagtaatggc actcagtgat gcggaaacca
    43561 ttgaagaagt ccaaatgatt tttgaacatg gacatatata accaatgttc tcagtaaaaa
    43621 agatgccttg catcaagtaa taacttcaac caagtgaaaa ataggagacg gtgacaagat
    43681 ttttgtcacc gctcctatta tttttgtgtt ttaataactc ttttttatat agatagcgca
    43741 catacctatg aataatagaa caatactcca tgtaccaagt gtcaagatac cattcatgaa
    43801 agtaacctgt tctgaagcta gtggagagaa aatatctcct cctacgatca cattttgaaa
    43861 atcaacataa gaagttggca agtaatgact gattgattct atccatccaa tcgaagaaaa
    43921 caagcgatac tctgctaaga aaagacaaag aagtgtacct aaaagattga ctagagaatg
    43981 ttcaaataaa gcagatagaa gaaaactgat cgtacttaaa aagaacaccc acaacaacaa
    44041 gaacagaaaa ctctggaaaa gatatgcata catcggaagg attcgtatct cctgaccaat
    44101 gatcgttcct acaggataac ggaagtcgcc caaaccattt ttgattacta ctagtataga
    44161 gacgaacaaa aatggaatca cgagacttag catagctgca ccataataaa cggtgaaacg
    44221 gatgctatgt ttctttacca tactatccgg taatagattg actagagaga ttacctgctt
    44281 tcttttttca taagagatga tcgaggaaag aaacaaggcg gttatacata aaatcaaaaa
    44341 actagaaatc attcctgaaa aaatattttc gtagtaattc gctaaaggga ggtgcgctgg
    44401 caagtcaaaa attgaatatc tttggatctg atgttctttc atataacgga gcaactctac
    44461 acgtttttgt tgttcaatga ttggaagtcc ttgtctttga ccagaaatca gccggcccag
    44521 tatatctttt tcataatgat aagttgcttc gaccatttcc tggacattcc ctgtgttttt
    44581 tgcacgtaaa attgcttcga tcagtttgtt gcttttctct aaactttcca caaattcttt
    44641 ctcattctca tcttctttca acgtttcaat caaacgttgc gtctcttcat agttatgatt
    44701 cagttcatcg aattctgtat aagcaagttc atgatcaaaa aaaagtggga atgcaaagag
    44761 cacgatcaac aatagaggga ataaaaagtt tactttgttt tttagtaaga gattgcgttc
    44821 aaatgtaaat aaagtcatag ttacacctca tacaattgac gataaatagc taaaatctct
    44881 tccatatttg aggattctgt gacgatttct ccatttttta gaaaacatgc tttagaacaa
    44941 atcgattgga tattcgcgat agcgtgggaa gaaagaagaa tcgtcttccc ctcttcctgc
    45001 cattgtctga tcagcctatt caagatatca atacttcctg ggtctaaccc gttcaacggt
    45061 tcatcgaata acatgacaga tgaatcactg atcaagtaag cagcaagcaa gacatgttgt
    45121 ttcattccta gagacatttt tccaatcggc aaagtaaggt actcagtcat cttcaatcgt
    45181 tggaaaacag tttggcattc tactttgctc tgccaatagt atttgatcaa agtcaaataa
    45241 tcaacagccg ttagatagtc ggataactga ttcgttgatt ctagaaaaaa gaaagaccgg
    45301 cgataatctg tataagcagt aggagagatg ttgtgaaggg acaattgatc ataagttgtt
    45361 ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc caaatccgtt cggggcaatc
    45421 aaagcagcta cttctcctga ttctactaaa agatccaatt gtcgaatcac acgtttttta
    45481 ccacgtttca tgttcatgtt tttgatcagt aatgtcatag atagatctcc aatataaatt
    45541 ttaatatcat aataaaacaa aaagcatcca aataaaatgc tgacaaaagt tattatttac
    45601 atattataaa aaagttaaaa taacatgttt atttaaaagt aaagtttgct gatcccaaaa
    45661 tttttttatt ttgctgatat cctcaaacct ttggcacaaa gtttcgtgcc aaagtacgac
    45721 tatttctctt tttagtaagc ggatacaatt agagtatcaa aaggagagag gaagagacaa
    45781 aatggaacgg atgattccca aaaacaacat ttcgctaaaa gcgagggtac aaaaattagg
    45841 cagtacctta tcaagcatgg tcatgccaaa cattggagca ctgattgctt ggggtgtgtt
    45901 aacggcttta tttatcccag atgggtattt gccaaatgaa gctttcgcta ccatggtaag
    45961 cccgatgcta acttatctga ttcctttatt gatcggctat actggcggaa aagtaattgc
    46021 tggtgaccgc ggatcagttg tgggagcaat tgcaacgatg ggtgtgattg tcggaacaga
    46081 tattccaatg atgctaggtg cgatgatcat gggacctctt ggtggatatg ctatcaagaa
    46141 atttgatcag ctgttccaaa aacggatcaa atccggtttt gaaatgttgg tcaataattt
    46201 ttcagcaggt cttatcggtt ttggattagc gttattaggt ttttctgcga ttggtccagt
    46261 tgtcgataca ttgactcaag caatggcaaa aggtgtagag atcattttat ctgcccattt
    46321 gattccattg acaagtattt ttattgaacc agcaaaaatc ttatttttga ataatgcaat
    46381 caatcatggg attttgacac cattaggaac tgaacaagta gcagatgcag gaaaatctat
    46441 tctattcttg cttgaagcaa atcctggtcc aggacttggc gtattacttg cttttacttt
    46501 gtttggcaaa ggagcagcaa aatcttcagc accaggtgct atgatcatcc actttttagg
    46561 tgggatccat gaaatctatt ttccttacgt catgatgaaa ccgttattat tcttatcagt
    46621 tataggcgga ggaatgtcag gaagtttcat tttccaattg atggatgctg gactaagagc
    46681 gccagcttct ccaggatcaa tcattgcgat tctagcgatg acaccgttca attcttacct
    46741 tcctgttatt ttgggcgtga ttgttgccgc agctgtttca ttcggtattt ctgcagtgat
    46801 tttgaaagcg gatgcgaaag atacaaatga agactttgaa aagtctgtgc aagagacaaa
    46861 agtagctaaa caagcatcta aaggtgccca aggtattcct caaggaactg cgctatctgg
    46921 cgtacaaaaa atcatttttg cttgtgatgc agggatggga tcaagtgcga tgggcgcttc
    46981 gattttgaga aaaaaagtac aggaagcaga cttgcctcaa acggtgacta accaagcaat
    47041 cagccaactg acagatgact cgaatacatt gatcgttact caggtcgaac tacaagaaag
    47101 agcaaaacaa aaagcaccga acgcgcgttt tgtttcagta gaaaactttt tgaattcacc
    47161 tcgatatgat gaaatcattc aagaattgag cagcaaacag gaaccatcaa cttctgaaac
    47221 taagaacgag ccaacagaag agtttctttc agcaaacatc aaacaaatca aagagattct
    47281 acttgtccac gatgatcgtt cgggatcagc aacaatggga atgactgtgc tcaaagatat
    47341 attagacaag aaccatttaa ctatcccgtt acgaaaagtc aacatccagg agttaaaagg
    47401 ggagcctgat tcactgatcg tagccaaaga aagtttagca gaaaaagcac agatggctgc
    47461 accgcaatcc attcatttat cggttaatag catggtcaac ccacaaaaat ttgaatcaat
    47521 tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca gcatgcaaag tttgccattc
    47581 gacatgatcg aactcaatcg atcatttaaa accaaagaag aagcgatccg ctattgcggt
    47641 caaaagcttg tagaagcagg atgcgttgat gaggaatata tcgatgccat ggtagaaaga
    47701 gacaacatgt tatctgttta catggggaat tttatcgcga ttcctcatgg aacagatgaa
    47761 gcaaaaaaat atgtgaaaaa atccggcatt tgtgtcgtac aggtgccaga cggtgtcaat
    47821 tttggtacgg aagaagaaga aaagattgcg actgttttgt tcggtatagc aggagtcggc
    47881 gatgagcatc tgcaacttgt acagcaaatc gcactttatt gcagtgatat ggacaatgtt
    47941 gtccaactag cagacgcgct cagcaaagaa gaagtaacgg aaaatctagc aattgcttaa
    48001 ggagagagaa aaatgagagc agtacatttt ggtgcaggaa atatcggtcg aggatttatt
    48061 ggagaaattt tggcaactaa tggatttgag attactttcg tagatgtgaa tcacacgatc
    48121 attgatgctt taaatgaaag aaaagaatac gaaatcgaat tagcagatga atcaaaagaa
    48181 agaatcactg tgaaaaatgt caaaggaatc aacaatcaaa caaatccaga aaaagtaatc
    48241 caagaaatcg ctgaagcaga tttagtcact accgccattg gcccgaatat cttacctttt
    48301 attgccgagt tgattgcaaa aggtatccaa gcaagagaaa tggaaggaaa cacaacacct
    48361 ttagatgtca ttgcctgtga aaacatgatt ggtggttcag catttctaga aaaagaagtg
    48421 tataaatatc ttccagaaac atcatttact gacaaatata tcggctttcc aaatgcagca
    48481 gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt tattcgtcca agtagaacct
    48541 ttcaaggaat gggtcattga cgatagccaa agaaaaaata aagaaatcca attaaaaggg
    48601 gttttatatg tagatgatct agaaccatat atcgaacgca aactatttag tgtcaatact
    48661 ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt accagaccat tgatgaagca
    48721 atgcaagatg cgttagttgt agcacaatta aaatcagttc tgcaggaaac aggaagtttg
    48781 ctgattgcca aatgggggtt tgattcagag cagcatcatg cgtatattga aaaaatcatc
    48841 catcgtttcc aaaacaaata tatctctgat gcgatcacac gtgtcgctcg gacaccatta
    48901 cgtaagttag gttatcaaga acggtttaca cggccaatga gagaactgca agaacataac
    48961 ttgacttgtc cacatttgac ggcaacaatg ggtattattt ttaattatta tgatcctgaa
    49021 gatgaacaaa gccgtcaact gcatgaaatg aagatccatg aaaacttaga acaattgatc
    49081 caagaagtca caggaatcaa tgatccgaaa acaatcggga atatcaagca aaacgtgaac
    49141 cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa ggggaatgtg acgcaacttt
    49201 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcaggtcca
    49261 actcttcacc gtaacgttgt tctaaagcta ggcgaatcaa gcgagctgct aatttctcat
    49321 ttcttgcaag gataggtccg tggaaataag aaccgaagac atttcggtaa atgacacctt
    49381 cggattgatc ttccccattg ttcccttgac cttgtaccac tttccccaaa ggccgttcac
    49441 ctttacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
    49501 cttcattatg gatgacaatg tcaccgataa accggttgtt ttcctgactt aatgtgtagt
    49561 gatccagggc actgatacct tggatctttt ctccgttggc tcccatgtag tagtgaccca
    49621 gcaattgata accaccgcaa atggctaaca tgacaccatc attttcgata tagttgatca
    49681 aagaatcttt ctttttctgg atgtcttttg aaatgatcag ttgctcaaag tcttgaccac
    49741 cgccaaagaa aaccaagtca tatttattag catcaaaagg ttcatgtata ctaacgatct
    49801 ccgtatggca agaaaccccc attttttctg ctacatattt caacatcaaa aggtttccgt
    49861 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
    49921 ccatgattca ttcctccttt gataaatcct tgagccgcta gttctttacg tagctgcaat
    49981 acagcagtgt aagtagctaa gatatagaca tgttcagttg gcaattcttt gattttttcg
    50041 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttc
    50101 agacgtaaag tcatatctgt atgacgatct cctccagaaa taacggctgg gatatccata
    50161 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc atctgcgtaa
    50221 ttggcattca ataaagctac taaagaaaat ggataaggag ctagaccaat catatcgatt
    50281 acttgattga tgcctacagg atttttgaca agtactaatg tacatttctt tccattgata
    50341 ttgatgactt cttgtcggcc gaacactttc tcgtcatatc ctaaaccttg tttgatttta
    50401 tcaggatcga cattgaagta ctccgccact gcagtagctg ctaaagcgtt gtagacgtta
    50461 tacataccac caacttcgat tccatattca tgaccatcaa tcacaaaatc tgctgaaaca
    50521 ttgtccatcc gtaccatatc agtcaatcgg taatccagtt caggacgttt gaagtcacaa
    50581 tttggacagt aatattttcc taagtttgca taagtgatca ttttataatg caggatgtga
    50641 tgacaattcg gacacaagac accgtctgta ttgtaatgcg ccatttgttc acgatcttct
    50701 tcatggtcaa agccataata ttttctagga ttaactgttt cgatcgaatt gaagatcggt
    50761 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
    50821 ttataggtcg tataaatttc tccataccga tccatttgat cacggaaaat atttgtaaat
    50881 aaaaatagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
    50941 aggacagcaa attttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
    51001 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
    51061 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtaac aacgacgact
    51121 tggtagtctt ttgccaagct atccaatata tgtggatcga ctttcagcgc tagtttacct
    51181 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt tttcccaact
    51241 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
    51301 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
    51361 aagaaaaggt tacggctgaa agtgcatgaa atcggagaaa accgcatgat ttaaaaaaaa
    51421 acagcttgaa acaaaatgat tgtttcaagc tggtgtgatt ttattattac tcttcatcgg
    51481 tcttttcatt attaaccaat aatgattttc tcacttggat attcatagcc cagatctcgg
    51541 gtttcttttg gtacgaacag catcaatgta tacagcattc caatccgccc aataaacatc
    51601 agggcagcaa tcgtgatttt ccctacaaca gacaagtcgc cggtaatccc taaggacaaa
    51661 ccagttgttc caaaagcaga agttacttcg acgatgatcg agatcagtgt ttgttcctct
    51721 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
    51781 actgactttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat attatcttca
    51841 ctcttgagga aggagtatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
    51901 cctcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtc
    51961 gtaatctgaa aatcacccaa atcgtgaatc tggagaccgg cattacgagt cgtaatcgaa
    52021 taaaacatcg aattgatcca tttgacactc atactggaat cttggaataa atggtccttt
    52081 tccaatagat aaattaagat agttcccgaa acaaacaaga tgacaaaagc caacacagcc
    52141 aatttggtaa acaaggaaaa acgaaatgga agctttgcac tggaacgttt atataatagc
    52201 cactcccgac actccatcaa tacaggaaaa ccaattccgc cgacaaaaat caaaaacatg
    52261 attaggatta agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
    52321 aatccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
    52381 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
    52441 tgaaaccaga ttaaaatagc aaaagtaatc cggatcagtc ggacgatacc agacaaacgc
    52501 ggttgattca tatccgtcat gatcagctgc cgctgtttca atgtgattcg tcgtttagaa
    52561 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
    52621 tccagcagta taatgccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
    52681 gtcacgctca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
    52741 ggttcgcgaa aaatcggcag ataaaataga acgagtgata agaccgtcat caatatataa
    52801 taaaaaacga tgatttggat cgatgagaaa tgattagcag caaacttctg tcctcgccgt
    52861 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
    52921 ctaccagtat acaaggggaa tcaggaatac tcaaagaaat tattgaacag ataacaatac
    52981 agctttcgat taaaatagaa atggcttata ataaagcaaa tcctagaaaa aagaaggagg
    53041 aggaataaca aattgatcaa tgcccaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
    53101 aattatgata cagttctaaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
    53161 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacgttgga attcatgtct
    53221 caatatgcag atatcacgtt atttttttca aattcgaata tccatcctaa aagtgagtat
    53281 ttgcggaggt tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
    53341 catgtagcgc tgattgttga tgaataccgt ccaagtgaat tcaccaaaat ggttttaaca
    53401 aaccatcttg aagacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
    53461 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
    53521 acgatctcac caaagaaaaa tgcagccttg atcaatcaaa tcggaatgga tatccaaaag
    53581 atttacggga cacaatattt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
    53641 ttgcaaatgt gcaaagagta tgatatctat cgccaatgtt attgtggttg cgtgttcgct
    53701 gccaagcagc aaggatgtga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
    53761 acatcgaccg tagtttttga tgaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
    53821 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagaa
    53881 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
    53941 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
    54001 ttccagctac cgtgcaccca gttcaagttc tagtactgta gaagaaacaa caccaactac
    54061 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
    54121 tacatgggac gacacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
    54181 aagcgaaaca agcaatacgg taccagatgg cacttcatcg gattcaacca atccaagtac
    54241 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
    54301 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
    54361 aacgtataac caacaagcag gtgattctca atgacatttt tactagacct tatcaatggg
    54421 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
    54481 attttaagtg tgatccccac gctttattta ttgagaattg tttatggact ttggcaaaac
    54541 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
    54601 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cacacagctt
    54661 tttgtgaagt atttgcctga tgaagcgttc aacatccaga cagaagaaac aatcaataat
    54721 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattac
    54781 caattgaaat tagccgagaa tatgcggtac ttgattggaa atggtgagat caaaataaat
    54841 gaactcggtc gcatggatgg atttcttgtc gatagaaata cgttgtatcc ttattactat
    54901 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
    54961 gaaaaagtag ggaccattcg gctgagctca acagaagaca cattagaacc tgtcggattg
    55021 ttcatcgttg gcggtgattt tgtcaaagac gggttgcgtt atcatgagcc ttatcgcctg
    55081 aaattaattg caaaaaaagc acaatcacaa gaagaacaag ttgtttatag tcggagccaa
    55141 agaagaagac attcataagg ggatgtgaca tgacttttgt catgccactt ttttatataa
    55201 cgagaaaaat agaacgaaca cgtcacttat tctttccttt catttataaa cattgtagaa
    55261 taaagagagt acaatgaaat atcgtaaaaa aatgtaataa ataacgagga aatataagta
    55321 ggaggtaagc tgtgaaagaa tttttgaaaa attgggggat tctgatcctt gtattagcgg
    55381 ctattttatt agctagagtg tacgtgttta caccagtcac tgtaaacgga cattcgatgg
    55441 accctacatt aagtgatgga caacgactga tttcatctaa aatctcgaac tatgaacgaa
    55501 tggacatcat tactacgaag gaaccagggg atgaagagcg aatgatcgtt aaacgaatca
    55561 ttggtatgcc tggggacaca gttaagatgg aaaatgacca gttgaccatt aatggcaaaa
    55621 agtatgatga gccttatcta gataagttca aaaaagaatt ttcagaagat aagttacaag
    55681 gagagtatgc ttatagttca gggttccaag cacaagcaga aagtgcctct acctttacga
    55741 gcgattttga atatactgtg ccaaaaggaa agtatcttgt tctgggtgat aaccgtttga
    55801 tttcaaaaga cagccgaatg tttgggttag tcgacaaaga tatgatccaa ggtaaagttg
    55861 ttttccgcta ctggcctctt tccgaaatca aaatcatttg atgaaaaaat aaaaagaaga
    55921 ggttgtgaca gaagtggtca gcttcaagaa ataagaaact gcttttggaa caccgtttaa
    55981 acgaaaaaag aagctgtgac atatttttgt cacagcttct ttttaagctt tttggctgat
    56041 ggataggaaa agcgaagcgt ttttgttcat aaatagcttt tctccacgat gaaaagtatg
    56101 actagcagta gagacaaagc cagttttttt caatcgatcg ttcaattctt cttggataaa
    56161 gccattatgg actttcggat gactgatttg ttcattcttg tcaaaatcta caataatcag
    56221 ttgaccgcca ggagctaaaa gttcgaaaag ttttatgaga atagtcttcg tatcaggaat
    56281 gtgcaaaagt acgagtgata gaaggatcac atctgcttgg atagcaggaa attcgacaga
    56341 agcatcagcg tggatcgttc ggacattttt cagatctgct gcttggatct tttcttctac
    56401 cattttcaac atagtttctg aagcatcggc aatgatcagt tctttgaatc tttccgcaag
    56461 cggtaaactc acaagcccag ttccgcctcc gtaatccagc aggacttttt ggtggctgtc
    56521 gcgcggtaga taagttgtta gttcttcgcg aataatcttg gctaatgctt gacggtcagg
    56581 ggaatcataa tgattagcga tggaatcaaa aatatttttt gtcatcaaat agctccttac
    56641 ataaaacttt gggattttaa cttattatag cacgtgaaaa gaaagaaaac atgctatact
    56701 caattcaaaa agaaagtatt tagcagatgt acttattttt ataaatccta agaattttca
    56761 aggacagaaa tttttctaga aagataagat aaatggatcg ccttatttag ttcttgtcaa
    56821 aaaattggaa caaaagtaag caagggattt gaacgataat ctgtgcttga tgaaaattct
    56881 cactgtaaaa aatgttgaaa atcaagtata ataccatagg aacaaactgg aaggtgaaaa
    56941 agagcatggc acaactattt tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga
    57001 tcctaaaagt cgcacataat tatgaagaac aaaataaacc agttgtcttg atgacaagcg
    57061 gtattgatac gagagatggt ttaggcgttg tttcaagccg aatcggactg aaacgtgaag
    57121 caatgccgat atttgaagaa acagacgtat ttgacgtgat tcagaacatg gaaagcaaac
    57181 cctattgtgt gctgatcgat gaagcgcagt tcttaaaaaa gaaacatgtc attgctttta
    57241 cgagaatcgt ggatgaattg gatattccgg ttatggcgtt tggtttgaaa aatgattttc
    57301 gaaacgagtt atttgaagga tcaaaatatt tattgcttta cgcagacaag attgaagaaa
    57361 tgaaaacgat ctgttggttc tgccacaaaa aagcaatcat gaatttacat tatattgatg
    57421 ggaaacctgt ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct tattaccccg
    57481 tttgtcgaaa acattacttt catccatcaa tgattacaga aggagaataa gcatgtacga
    57541 tcaattacaa tcaattgaag accgttatga agagcttgga gaacttttaa gtgacccaga
    57601 agtgatttct gatacaaagc gctttatgca actttcaaaa gaagaagcaa acacgcgcga
    57661 aacagtcgaa gtttatcgtc gttataaaga agtagtacaa gggatcaccg atacagaaga
    57721 actgcttggt gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag aattatctga
    57781 gttaaaaaaa gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt taccaaaaga
    57841 tccaaatgac gataaaaata tcatcatgga aatccgcgga gcagcaggtg gagacgaagc
    57901 agccttattc gcgggtgatc ttttcaatat gtaccaaaag tatgctgaag cacaaggttg
    57961 gaaaacagaa gtgatggaag caaatgttac cgggatcggt ggatacaaag aagtgattat
    58021 gatgattacc ggagagaatg tttattcgaa attaaaatat gaaagtggtg cacatcgtgt
    58081 ccaacgtgtt ccttcaactg aatcgcaagg gcgtatccat acttccacag cgacagttgt
    58141 cgtgatgcca gaagctgaag aagtagagat cgatatcgaa gacaaagata tccgcacaga
    58201 tatctatcat gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat ctgcggtacg
    58261 tttgactcac ttaccaacag ggattgtcgt tgctatgcag gatgaacgtt ctcagatcaa
    58321 gaaccgtgaa aaagcaatga agatcttgcg tgctcgtgtg tatgataaga tccaacaaga
    58381 agcacaaagt gaatatgatg ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga
    58441 acggattcgg acgtataatt tcccacaaaa tcgcgtgact gatcatcgta tcggattaac
    58501 tatccaaaaa ttagatcaga ttttagcagg gaaattggat gaaatcgttg atgcattgat
    58561 catgtatgat caaacatcaa aactggaagc tatgcaaaat gggtaaaatg acctatcgag
    58621 aagtcctgtc acgggcttct tcttttttag aggatcatgg aaaagaagca tacagtatcc
    58681 aatttttatt tttggaacga aaacaatgga aaaaacttga ttggctcctt catatgaacg
    58741 aagagatttc agaagaagaa caacagttga tcgaaacaga tctgcgactt ctgcttgcag
    58801 atcatccgcc tcagtatttg ctaggatatg cagattttta tgatcatcgg ttgaaggtaa
    58861 ccgaagcaac actgattcct cgaccagaaa cagaagagtt agtggagtgg tgtttagacg
    58921 agacgcctga tgtaccattg gaagtgatag atatcgggac agggactggg gcgattgcta
    58981 ttagtttgaa agcagcaaga aaaaattggt atgtatctgc cgtagatctt tctgaagaag
    59041 cattagaagt cgcgaaagaa aatgctcagc acgaaggaac gaaaatcagt ttttaccatg
    59101 ggaatacatt ggaaccagta caggatcggc gatttgatgt gattatttct aatccgccgt
    59161 acatcagtcg gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc gaacctaaga
    59221 tggccctttt tgcggagaac gatggattag ctgtttatga aaaaatagca gttgaagcac
    59281 caagcgtact aaaaccaaat ggaaagattt ttttagaaat cggatttcgc caaggtgaag
    59341 cagtaaaaaa aatattccag caagcatttc cagataaaaa agtggcgata aaaaaggatc
    59401 tttttggtaa tgaacgaatg atccaagtct acagttgaag ctgtggatga aaaaattttt
    59461 atttgttttt ttattataaa aaaacactaa tttgacaagg atgtaaacga ctttttggct
    59521 tatcaacaag tttatccact attgacaaac gaatgatcca cagtcagtgg aaaacttgag
    59581 gataaagttg gataaaacaa gaacgtatga aaaaggggtt gtggataaac tgtggaaacg
    59641 aaacgttatg atgaaactga attaaaagag gcagccaaag cactgaaagc aggagaactt
    59701 gttgcttttc ctactgaaac agtgtatgga ttgggcgcca atgctctatt gccaaatact
    59761 gtcaagaaag tgttttctgt aaaaggaaga cctcaagaca atccattgat cgtccatgtc
    59821 gcctcttttg aacaagtaaa agagtatgtg gacaactttc atccagaaac agagaagatt
    59881 gtgaagaact tctggcctgg accactaact ttgatattta aaatcaaaaa agataccttg
    59941 ccctcagttg taacaggagg attatccaca gctgcatttc gtatgccgga taacaaaaaa
    60001 acactagaag taatcgaact ttcaggggtt ccgcttgtcg gtccaagtgc aaatacgtcg
    60061 ggaaaaccaa gtccgacaac tgcagatcat gtattccatg atctacaagg aaaaattacc
    60121 gggatcattg atgatggagc tacgcgaatt ggagtagaat ccactgttct tgatttaagt
    60181 gatccaacag ccatgcctat gatcttacga ccaggtgcag taacgaaaga acaaatcgaa
    60241 gcagtcatcg aatctcctgt tgctattgat cagcatcttg taaaagaaaa tgaaacacca
    60301 aaagcgcctg ggatgaaata caagcactac tcacccgata cacgtgttct gatggtcaga
    60361 gagggcgatt ggtcaactgc agttcaatgg gcaaaaaata agaagatccg tgtaggtgtc
    60421 atagcaagcc ccgaaatttc ggatcaagtg cggacagata ctgcagctgt ttatatgtat
    60481 aacgacaact cagtagaagc ggcggctaaa ggattgtttg caggtctaag aggattagat
    60541 gaaccaacac ttgggttaga tttgattttt gtacaagtat atccagaaac tggattgggg
    60601 aatgcttata tgaatcgctt gaaaaaagca gctggacaaa attattttga aaaatgatca
    60661 attggttatt gccgcattct tttagtatga tacaatgcta tcaagtaaat cttctatcag
    60721 tgaacgaaaa acgaagtgta aatagaagag atacgaatat ttggcaattt gtttttgatg
    60781 ggaaaaataa aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg
    60841 tttgaccctg atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg
    60901 gagctgattg cctctgaaaa ctttgtctcc gaagcagtga tggctgcgca aggaagtatt
    60961 ttgacaaata aatacgcaga aggttatcca gggcatcgct attatggagg atgtgaattt
    61021 gtcgacatcg ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt
    61081 gcgaacgtcc aacctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta
    61141 gaaccagggg acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga
    61201 tcaccagtca acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca
    61261 acagaagtta ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg
    61321 attgtagcag gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc
    61381 gcagatgaag taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca
    61441 gcaggacttc atcctaatcc agttccttat gcagatatca caacgactac cactcacaaa
    61501 acattgcgcg gacctcgagg cggcatgatt ttgacaaacg atgaaacatt agcgaaaaaa
    61561 atcaatagtg cggtcttccc tggtattcaa ggcggacctc tagagcatgt gattgctggg
    61621 aaggcagcgg cattcaaaga agcactagat cctgcattta aagaatatag tgaacaaatc
    61681 attgccaatg caaaagcaat ggtcaaagta ttcaatcaag caatcggaac aagagtcatt
    61741 tcaggagcga cagataatca tctgatgctg atcgatgttc gtgagttagg tataaatgga
    61801 aaagaagcag agagtatttt ggacagtgtg aatatcacgg taaataaaaa ctcgattcct
    61861 tttgaaacgc taagtccatt caaaacaagc ggcatccgaa tcggtacacc agcaatcact
    61921 actcgcgggc ttaaagaaga agatgctgta aaagtggctg aattagttgt taaagcatta
    61981 caagcaaaag gcgacaatgc tcaattagac gaagtcaaaa caggtgtacg cgaattaact
    62041 gaaaaatttc cattgcataa aaaataaaaa tttaaa
//
LOCUS       DADJYG010000004        50942 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.004, whole genome shotgun sequence.
ACCESSION   DADJYG010000004 DADJYG010000000
VERSION     DADJYG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.004"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..478)
                     /locus_tag="JZ382_000171"
     CDS             complement(<1..478)
                     /locus_tag="JZ382_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HAY2426385.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIG"
     gene            1097..3931
                     /locus_tag="JZ382_000172"
     CDS             1097..3931
                     /locus_tag="JZ382_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2426386.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            4122..4613
                     /locus_tag="JZ382_000173"
     CDS             4122..4613
                     /locus_tag="JZ382_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAY2426387.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            4716..5705
                     /gene="manX"
                     /locus_tag="JZ382_000174"
     CDS             4716..5705
                     /gene="manX"
                     /locus_tag="JZ382_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY2426388.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            5738..6541
                     /locus_tag="JZ382_000175"
     CDS             5738..6541
                     /locus_tag="JZ382_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY2426389.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            6562..7476
                     /locus_tag="JZ382_000176"
     CDS             6562..7476
                     /locus_tag="JZ382_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY2426390.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            7559..7933
                     /locus_tag="JZ382_000177"
     CDS             7559..7933
                     /locus_tag="JZ382_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAY2426391.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8072..8317
                     /locus_tag="JZ382_000178"
     CDS             8072..8317
                     /locus_tag="JZ382_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY2426392.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            8489..9553
                     /locus_tag="JZ382_000179"
     CDS             8489..9553
                     /locus_tag="JZ382_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAY2426393.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9799..10224
                     /locus_tag="JZ382_000180"
     CDS             9799..10224
                     /locus_tag="JZ382_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAY2426394.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            10217..10666
                     /gene="nusB"
                     /locus_tag="JZ382_000181"
     CDS             10217..10666
                     /gene="nusB"
                     /locus_tag="JZ382_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY2426395.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10763..11617
                     /locus_tag="JZ382_000182"
     CDS             10763..11617
                     /locus_tag="JZ382_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAY2426396.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            11618..12955
                     /locus_tag="JZ382_000183"
     CDS             11618..12955
                     /locus_tag="JZ382_000183"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY2426397.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12960..13184
                     /locus_tag="JZ382_000184"
     CDS             12960..13184
                     /locus_tag="JZ382_000184"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY2426398.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            13186..14076
                     /locus_tag="JZ382_000185"
     CDS             13186..14076
                     /locus_tag="JZ382_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAY2426399.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            14085..14903
                     /locus_tag="JZ382_000186"
     CDS             14085..14903
                     /locus_tag="JZ382_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAY2426400.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            15010..15459
                     /locus_tag="JZ382_000187"
     CDS             15010..15459
                     /locus_tag="JZ382_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAY2426401.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            15477..17159
                     /gene="recN"
                     /locus_tag="JZ382_000188"
     CDS             15477..17159
                     /gene="recN"
                     /locus_tag="JZ382_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY2426402.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(17214..17330)
                     /locus_tag="JZ382_000189"
     CDS             complement(17214..17330)
                     /locus_tag="JZ382_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAY2426403.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(17403..18359)
                     /locus_tag="JZ382_000190"
     CDS             complement(17403..18359)
                     /locus_tag="JZ382_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAY2426404.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            18530..18898
                     /locus_tag="JZ382_000191"
     CDS             18530..18898
                     /locus_tag="JZ382_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAY2426405.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            19068..19499
                     /gene="mraZ"
                     /locus_tag="JZ382_000192"
     CDS             19068..19499
                     /gene="mraZ"
                     /locus_tag="JZ382_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY2426406.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            19520..20479
                     /gene="rsmH"
                     /locus_tag="JZ382_000193"
     CDS             19520..20479
                     /gene="rsmH"
                     /locus_tag="JZ382_000193"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY2426407.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            20484..20885
                     /gene="ftsL"
                     /locus_tag="JZ382_000194"
     CDS             20484..20885
                     /gene="ftsL"
                     /locus_tag="JZ382_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY2426408.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20886..23078
                     /locus_tag="JZ382_000195"
     CDS             20886..23078
                     /locus_tag="JZ382_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAY2426409.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            23104..24066
                     /locus_tag="JZ382_000196"
     CDS             23104..24066
                     /locus_tag="JZ382_000196"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY2426410.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            24080..25441
                     /locus_tag="JZ382_000197"
     CDS             24080..25441
                     /locus_tag="JZ382_000197"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY2426411.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            25467..26555
                     /gene="murG"
                     /locus_tag="JZ382_000198"
     CDS             25467..26555
                     /gene="murG"
                     /locus_tag="JZ382_000198"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY2426412.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            26570..27790
                     /locus_tag="JZ382_000199"
     CDS             26570..27790
                     /locus_tag="JZ382_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAY2426413.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27946..29271
                     /gene="ftsA"
                     /locus_tag="JZ382_000200"
     CDS             27946..29271
                     /gene="ftsA"
                     /locus_tag="JZ382_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY2426414.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            29294..30535
                     /gene="ftsZ"
                     /locus_tag="JZ382_000201"
     CDS             29294..30535
                     /gene="ftsZ"
                     /locus_tag="JZ382_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY2426415.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            30552..31229
                     /locus_tag="JZ382_000202"
     CDS             30552..31229
                     /locus_tag="JZ382_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAY2426416.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            31243..31842
                     /locus_tag="JZ382_000203"
     CDS             31243..31842
                     /locus_tag="JZ382_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAY2426417.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31857..32144
                     /locus_tag="JZ382_000204"
     CDS             31857..32144
                     /locus_tag="JZ382_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAY2426418.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            32193..32975
                     /locus_tag="JZ382_000205"
     CDS             32193..32975
                     /locus_tag="JZ382_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAY2426419.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32996..33703
                     /locus_tag="JZ382_000206"
     CDS             32996..33703
                     /locus_tag="JZ382_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAY2426420.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33965..36763
                     /gene="ileS"
                     /locus_tag="JZ382_000207"
     CDS             33965..36763
                     /gene="ileS"
                     /locus_tag="JZ382_000207"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY2426421.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36812..38338)
                     /locus_tag="JZ382_000208"
     CDS             complement(36812..38338)
                     /locus_tag="JZ382_000208"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY2426422.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(38353..39000)
                     /locus_tag="JZ382_000209"
     CDS             complement(38353..39000)
                     /locus_tag="JZ382_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAY2426423.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(39184..39513)
                     /locus_tag="JZ382_000210"
     CDS             complement(39184..39513)
                     /locus_tag="JZ382_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAY2426424.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            39690..40418
                     /gene="sfsA"
                     /locus_tag="JZ382_000211"
     CDS             39690..40418
                     /gene="sfsA"
                     /locus_tag="JZ382_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY2426425.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            40434..41447
                     /locus_tag="JZ382_000212"
     CDS             40434..41447
                     /locus_tag="JZ382_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2426426.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            41447..42724
                     /locus_tag="JZ382_000213"
     CDS             41447..42724
                     /locus_tag="JZ382_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY2426427.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42787..45489
                     /locus_tag="JZ382_000214"
     CDS             42787..45489
                     /locus_tag="JZ382_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2426428.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            45641..45958
                     /locus_tag="JZ382_000215"
     CDS             45641..45958
                     /locus_tag="JZ382_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2426429.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45988..46308
                     /locus_tag="JZ382_000216"
     CDS             45988..46308
                     /locus_tag="JZ382_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAY2426430.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            46416..47876
                     /locus_tag="JZ382_000217"
     CDS             46416..47876
                     /locus_tag="JZ382_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426431.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47944..48165
                     /locus_tag="JZ382_000218"
     CDS             47944..48165
                     /locus_tag="JZ382_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426432.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            48196..48378
                     /locus_tag="JZ382_000219"
     CDS             48196..48378
                     /locus_tag="JZ382_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAY2426433.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            48378..48791
                     /locus_tag="JZ382_000220"
     CDS             48378..48791
                     /locus_tag="JZ382_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAY2426434.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48914..50095
                     /locus_tag="JZ382_000221"
     CDS             48914..50095
                     /locus_tag="JZ382_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426435.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            50484..50557
                     /locus_tag="JZ382_000222"
     tRNA            50484..50557
                     /locus_tag="JZ382_000222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50518..50520,aa:Arg,seq:cct)
     gene            complement(50626..>50942)
                     /locus_tag="JZ382_000223"
     CDS             complement(50626..>50942)
                     /locus_tag="JZ382_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2426436.1"
                     /translation="KNPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLR
                     HTHASILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKN
                     DN"
BASE COUNT        16929 a         8606 c        10933 g        14474 t
ORIGIN      
        1 tgcctatcat tttttgttga gctacaatta aacgtttatt agttaatgcg tatgcataat
       61 tattgtcatg cttagttgat gagatataat tatgcaatcc tacaaagcag aagattacgt
      121 attcgtcact gttaagttga tctgatatta agttaaaatg atgagttgtc cattttttag
      181 tcattccttt accgtagccg tatttaagac aaaattctac catctcttca gtggttctca
      241 ttctatcatt acctatatca tgattgtttt gtgcagtatg atcagatatg tttaattttc
      301 ttttttctga tggatcctta tctaaatctt ctaaattcaa agatcttatt attttataag
      361 gagtagtcat accgtaacca gcttttttca tacaattgcc gcaaacaatt tgtttatctt
      421 tgattttata tgaagttaag ccaattttac ctccgcactt tgcacattgt ttactcacat
      481 tagttcctcc tagatatcag ctttctacta ctattaacat tctatcaata gtgaccatac
      541 tttgaccata ctttttagaa agaatagaaa attccaagat atttaataat agaagaaaag
      601 cactaacatc atgggttcag aagaattgag agcttatcag gaaacagtat aattgaagtg
      661 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
      721 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
      781 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
      841 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
      901 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
      961 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     1021 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     1081 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     1141 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     1201 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     1261 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     1321 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     1381 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     1441 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     1501 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     1561 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     1621 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     1681 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     1741 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     1801 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     1861 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     1921 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     1981 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     2041 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     2101 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     2161 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     2221 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     2281 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     2341 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     2401 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     2461 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     2521 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     2581 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     2641 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     2701 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     2761 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     2821 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     2881 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     2941 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     3001 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     3061 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     3121 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     3181 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     3241 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     3301 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     3361 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     3421 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     3481 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     3541 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     3601 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     3661 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     3721 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     3781 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     3841 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     3901 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     3961 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     4021 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     4081 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     4141 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     4201 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     4261 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     4321 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     4381 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     4441 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     4501 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     4561 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     4621 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     4681 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     4741 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     4801 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     4861 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     4921 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     4981 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     5041 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     5101 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     5161 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     5221 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     5281 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     5341 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     5401 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     5461 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     5521 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     5581 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     5641 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     5701 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     5761 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     5821 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     5881 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     5941 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     6001 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     6061 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     6121 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     6181 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     6241 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     6301 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     6361 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     6421 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     6481 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
     6541 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     6601 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     6661 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     6721 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     6781 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     6841 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     6901 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     6961 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     7021 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
     7081 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
     7141 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
     7201 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
     7261 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
     7321 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
     7381 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
     7441 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
     7501 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
     7561 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
     7621 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
     7681 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
     7741 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
     7801 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
     7861 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
     7921 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
     7981 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
     8041 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
     8101 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
     8161 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
     8221 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
     8281 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
     8341 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
     8401 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
     8461 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
     8521 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
     8581 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
     8641 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
     8701 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
     8761 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
     8821 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
     8881 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
     8941 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
     9001 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
     9061 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
     9121 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
     9181 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
     9241 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
     9301 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
     9361 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
     9421 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
     9481 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
     9541 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
     9601 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
     9661 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
     9721 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
     9781 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
     9841 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
     9901 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
     9961 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    10021 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    10081 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    10141 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    10201 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    10261 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    10321 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    10381 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    10441 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    10501 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    10561 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    10621 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    10681 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    10741 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    10801 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    10861 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    10921 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    10981 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    11041 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    11101 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    11161 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    11221 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    11281 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    11341 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    11401 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    11461 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    11521 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    11581 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    11641 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    11701 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    11761 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    11821 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    11881 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    11941 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    12001 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    12061 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    12121 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    12181 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    12241 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    12301 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    12361 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    12421 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    12481 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    12541 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    12601 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    12661 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    12721 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    12781 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    12841 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    12901 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    12961 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    13021 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    13081 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    13141 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    13201 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    13261 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    13321 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    13381 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    13441 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    13501 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    13561 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    13621 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    13681 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    13741 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    13801 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    13861 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    13921 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    13981 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    14041 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    14101 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    14161 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    14221 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    14281 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    14341 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    14401 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    14461 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    14521 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    14581 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    14641 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    14701 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    14761 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    14821 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    14881 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    14941 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    15001 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    15061 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    15121 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    15181 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    15241 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    15301 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    15361 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    15421 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    15481 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    15541 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    15601 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    15661 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    15721 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    15781 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    15841 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    15901 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    15961 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    16021 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    16081 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    16141 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    16201 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    16261 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    16321 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    16381 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    16441 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    16501 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    16561 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    16621 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    16681 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    16741 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    16801 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    16861 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    16921 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    16981 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    17041 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    17101 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    17161 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    17221 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    17281 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    17341 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    17401 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    17461 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    17521 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    17581 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    17641 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    17701 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    17761 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    17821 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    17881 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    17941 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    18001 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    18061 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    18121 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    18181 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    18241 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    18301 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    18361 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    18421 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    18481 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    18541 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    18601 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    18661 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    18721 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    18781 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    18841 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    18901 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    18961 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    19021 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    19081 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    19141 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    19201 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    19261 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    19321 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    19381 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    19441 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    19501 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    19561 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    19621 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    19681 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    19741 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    19801 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    19861 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    19921 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    19981 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    20041 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    20101 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    20161 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    20221 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    20281 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    20341 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    20401 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    20461 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    20521 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    20581 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    20641 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    20701 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    20761 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    20821 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    20881 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    20941 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    21001 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    21061 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    21121 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    21181 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    21241 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    21301 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    21361 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    21421 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    21481 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    21541 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    21601 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    21661 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    21721 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    21781 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    21841 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    21901 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    21961 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    22021 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    22081 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    22141 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    22201 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    22261 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    22321 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    22381 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    22441 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    22501 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    22561 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    22621 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    22681 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    22741 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    22801 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    22861 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    22921 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    22981 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    23041 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    23101 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    23161 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    23221 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    23281 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    23341 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    23401 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    23461 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    23521 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    23581 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    23641 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    23701 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    23761 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    23821 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    23881 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    23941 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    24001 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    24061 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    24121 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    24181 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    24241 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    24301 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    24361 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    24421 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    24481 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    24541 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    24601 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    24661 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    24721 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    24781 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    24841 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    24901 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    24961 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    25021 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    25081 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    25141 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    25201 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    25261 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    25321 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    25381 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    25441 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    25501 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    25561 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    25621 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    25681 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    25741 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    25801 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    25861 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    25921 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    25981 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    26041 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    26101 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    26161 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    26221 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    26281 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    26341 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    26401 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    26461 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    26521 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    26581 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    26641 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    26701 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    26761 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    26821 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    26881 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    26941 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    27001 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    27061 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    27121 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    27181 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    27241 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    27301 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    27361 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    27421 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    27481 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    27541 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    27601 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    27661 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    27721 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    27781 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    27841 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    27901 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    27961 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    28021 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    28081 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    28141 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    28201 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    28261 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    28321 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    28381 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    28441 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    28501 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    28561 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    28621 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    28681 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    28741 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    28801 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    28861 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    28921 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    28981 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    29041 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    29101 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    29161 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    29221 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    29281 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    29341 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    29401 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    29461 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    29521 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    29581 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    29641 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    29701 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    29761 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    29821 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    29881 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    29941 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    30001 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    30061 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    30121 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    30181 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    30241 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    30301 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    30361 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    30421 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    30481 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    30541 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    30601 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    30661 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    30721 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    30781 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    30841 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    30901 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    30961 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    31021 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    31081 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    31141 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    31201 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    31261 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    31321 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    31381 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    31441 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    31501 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    31561 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    31621 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    31681 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    31741 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    31801 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    31861 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    31921 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    31981 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    32041 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    32101 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    32161 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    32221 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    32281 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    32341 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    32401 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    32461 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    32521 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    32581 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    32641 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    32701 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    32761 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    32821 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    32881 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    32941 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    33001 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    33061 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    33121 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    33181 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    33241 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    33301 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    33361 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    33421 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    33481 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    33541 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    33601 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    33661 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    33721 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    33781 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    33841 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    33901 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    33961 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    34021 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    34081 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    34141 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    34201 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    34261 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    34321 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    34381 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    34441 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    34501 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    34561 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    34621 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    34681 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    34741 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    34801 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    34861 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    34921 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    34981 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    35041 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    35101 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    35161 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    35221 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    35281 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    35341 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    35401 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    35461 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    35521 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    35581 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    35641 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    35701 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    35761 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    35821 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    35881 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    35941 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    36001 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    36061 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    36121 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    36181 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    36241 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    36301 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    36361 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    36421 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    36481 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    36541 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    36601 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    36661 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    36721 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    36781 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    36841 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    36901 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    36961 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    37021 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    37081 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    37141 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    37201 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    37261 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    37321 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    37381 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    37441 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    37501 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    37561 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    37621 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    37681 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    37741 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    37801 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    37861 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    37921 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    37981 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    38041 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    38101 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    38161 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    38221 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    38281 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    38341 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    38401 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    38461 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    38521 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    38581 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    38641 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    38701 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    38761 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    38821 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    38881 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    38941 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    39001 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    39061 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    39121 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    39181 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    39241 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    39301 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    39361 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    39421 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    39481 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    39541 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    39601 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    39661 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    39721 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    39781 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    39841 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    39901 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    39961 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    40021 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    40081 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    40141 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    40201 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    40261 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    40321 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    40381 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    40441 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    40501 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    40561 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    40621 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    40681 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    40741 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    40801 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    40861 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    40921 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    40981 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    41041 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    41101 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    41161 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    41221 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    41281 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    41341 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    41401 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    41461 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    41521 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    41581 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    41641 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    41701 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    41761 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    41821 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    41881 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    41941 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    42001 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    42061 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    42121 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    42181 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    42241 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    42301 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    42361 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    42421 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    42481 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    42541 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    42601 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    42661 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    42721 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    42781 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    42841 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    42901 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    42961 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    43021 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    43081 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    43141 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    43201 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    43261 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    43321 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    43381 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    43441 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    43501 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    43561 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    43621 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    43681 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    43741 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    43801 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    43861 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    43921 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    43981 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    44041 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    44101 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    44161 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    44221 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    44281 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    44341 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    44401 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    44461 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    44521 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    44581 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    44641 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    44701 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    44761 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    44821 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    44881 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    44941 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    45001 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    45061 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    45121 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    45181 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    45241 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    45301 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    45361 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    45421 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    45481 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    45541 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    45601 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    45661 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    45721 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    45781 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    45841 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    45901 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    45961 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    46021 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    46081 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    46141 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    46201 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    46261 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    46321 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    46381 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    46441 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    46501 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    46561 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    46621 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    46681 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    46741 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    46801 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    46861 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    46921 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    46981 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    47041 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    47101 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    47161 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    47221 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    47281 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    47341 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    47401 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    47461 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    47521 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    47581 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    47641 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    47701 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    47761 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    47821 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    47881 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    47941 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    48001 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    48061 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    48121 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    48181 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    48241 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    48301 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    48361 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    48421 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    48481 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    48541 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    48601 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    48661 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    48721 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    48781 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    48841 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    48901 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    48961 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    49021 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    49081 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    49141 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    49201 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    49261 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    49321 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    49381 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    49441 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    49501 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    49561 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    49621 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    49681 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    49741 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    49801 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    49861 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    49921 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    49981 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    50041 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    50101 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    50161 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    50221 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    50281 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    50341 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    50401 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    50461 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    50521 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    50581 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    50641 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    50701 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    50761 gctaagtata tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg
    50821 caaagtaaat tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac
    50881 ggcgtttgat gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt
    50941 ga
//
LOCUS       DADJYG010000005        48031 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.005, whole genome shotgun sequence.
ACCESSION   DADJYG010000005 DADJYG010000000
VERSION     DADJYG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.005"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..465
                     /locus_tag="JZ382_000224"
     CDS             <1..465
                     /locus_tag="JZ382_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY2426437.1"
                     /translation="EQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFR
                     KAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQ
                     DYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            563..751
                     /locus_tag="JZ382_000225"
     CDS             563..751
                     /locus_tag="JZ382_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2426438.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(809..1162)
                     /locus_tag="JZ382_000226"
     CDS             complement(809..1162)
                     /locus_tag="JZ382_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2426439.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1693..2115
                     /locus_tag="JZ382_000227"
     CDS             1693..2115
                     /locus_tag="JZ382_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY2426440.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2112..2342
                     /locus_tag="JZ382_000228"
     CDS             2112..2342
                     /locus_tag="JZ382_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2426441.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2840..3040
                     /locus_tag="JZ382_000229"
     CDS             2840..3040
                     /locus_tag="JZ382_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAY2426442.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3067..4260
                     /locus_tag="JZ382_000230"
     CDS             3067..4260
                     /locus_tag="JZ382_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2426443.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4328..5893)
                     /gene="guaA"
                     /locus_tag="JZ382_000231"
     CDS             complement(4328..5893)
                     /gene="guaA"
                     /locus_tag="JZ382_000231"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY2426444.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5952..6875)
                     /locus_tag="JZ382_000232"
     CDS             complement(5952..6875)
                     /locus_tag="JZ382_000232"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY2426445.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7071..7676
                     /locus_tag="JZ382_000233"
     CDS             7071..7676
                     /locus_tag="JZ382_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2426446.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7984..9000
                     /locus_tag="JZ382_000234"
     CDS             7984..9000
                     /locus_tag="JZ382_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2426447.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9141..10442
                     /locus_tag="JZ382_000235"
     CDS             9141..10442
                     /locus_tag="JZ382_000235"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAY2426448.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10488..11150
                     /gene="deoC"
                     /locus_tag="JZ382_000236"
     CDS             10488..11150
                     /gene="deoC"
                     /locus_tag="JZ382_000236"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY2426449.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11171..11569
                     /locus_tag="JZ382_000237"
     CDS             11171..11569
                     /locus_tag="JZ382_000237"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY2426450.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11635..12717
                     /locus_tag="JZ382_000238"
     CDS             11635..12717
                     /locus_tag="JZ382_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2426451.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12929..14491
                     /locus_tag="JZ382_000239"
     CDS             12929..14491
                     /locus_tag="JZ382_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426452.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14484..15617
                     /locus_tag="JZ382_000240"
     CDS             14484..15617
                     /locus_tag="JZ382_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426453.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15617..16573
                     /locus_tag="JZ382_000241"
     CDS             15617..16573
                     /locus_tag="JZ382_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426454.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16672..17838
                     /gene="deoB"
                     /locus_tag="JZ382_000242"
     CDS             16672..17838
                     /gene="deoB"
                     /locus_tag="JZ382_000242"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY2426455.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17862..18680
                     /locus_tag="JZ382_000243"
     CDS             17862..18680
                     /locus_tag="JZ382_000243"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY2426456.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18697..19404
                     /gene="deoD"
                     /locus_tag="JZ382_000244"
     CDS             18697..19404
                     /gene="deoD"
                     /locus_tag="JZ382_000244"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY2426457.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19702..20388)
                     /gene="gpmA"
                     /locus_tag="JZ382_000245"
     CDS             complement(19702..20388)
                     /gene="gpmA"
                     /locus_tag="JZ382_000245"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY2426458.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20560..21141)
                     /locus_tag="JZ382_000246"
     CDS             complement(20560..21141)
                     /locus_tag="JZ382_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2426459.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21274..21957)
                     /gene="rpiA"
                     /locus_tag="JZ382_000247"
     CDS             complement(21274..21957)
                     /gene="rpiA"
                     /locus_tag="JZ382_000247"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY2426460.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22276..22689
                     /gene="rpsL"
                     /locus_tag="JZ382_000248"
     CDS             22276..22689
                     /gene="rpsL"
                     /locus_tag="JZ382_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY2426461.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22773..23243
                     /gene="rpsG"
                     /locus_tag="JZ382_000249"
     CDS             22773..23243
                     /gene="rpsG"
                     /locus_tag="JZ382_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY2426462.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23337..25424
                     /gene="fusA"
                     /locus_tag="JZ382_000250"
     CDS             23337..25424
                     /gene="fusA"
                     /locus_tag="JZ382_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY2426463.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25596..26783
                     /gene="tuf"
                     /locus_tag="JZ382_000251"
     CDS             25596..26783
                     /gene="tuf"
                     /locus_tag="JZ382_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY2426464.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            27190..27498
                     /gene="rpsJ"
                     /locus_tag="JZ382_000252"
     CDS             27190..27498
                     /gene="rpsJ"
                     /locus_tag="JZ382_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY2426465.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            27529..28158
                     /gene="rplC"
                     /locus_tag="JZ382_000253"
     CDS             27529..28158
                     /gene="rplC"
                     /locus_tag="JZ382_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY2426466.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            28186..28809
                     /gene="rplD"
                     /locus_tag="JZ382_000254"
     CDS             28186..28809
                     /gene="rplD"
                     /locus_tag="JZ382_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY2426467.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28809..29099
                     /gene="rplW"
                     /locus_tag="JZ382_000255"
     CDS             28809..29099
                     /gene="rplW"
                     /locus_tag="JZ382_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY2426468.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            29138..29971
                     /gene="rplB"
                     /locus_tag="JZ382_000256"
     CDS             29138..29971
                     /gene="rplB"
                     /locus_tag="JZ382_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY2426469.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            30014..30292
                     /gene="rpsS"
                     /locus_tag="JZ382_000257"
     CDS             30014..30292
                     /gene="rpsS"
                     /locus_tag="JZ382_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY2426470.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            30315..30662
                     /gene="rplV"
                     /locus_tag="JZ382_000258"
     CDS             30315..30662
                     /gene="rplV"
                     /locus_tag="JZ382_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY2426471.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30676..31332
                     /gene="rpsC"
                     /locus_tag="JZ382_000259"
     CDS             30676..31332
                     /gene="rpsC"
                     /locus_tag="JZ382_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY2426472.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            31335..31769
                     /gene="rplP"
                     /locus_tag="JZ382_000260"
     CDS             31335..31769
                     /gene="rplP"
                     /locus_tag="JZ382_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY2426473.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31759..31947
                     /gene="rpmC"
                     /locus_tag="JZ382_000261"
     CDS             31759..31947
                     /gene="rpmC"
                     /locus_tag="JZ382_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY2426474.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31972..32238
                     /gene="rpsQ"
                     /locus_tag="JZ382_000262"
     CDS             31972..32238
                     /gene="rpsQ"
                     /locus_tag="JZ382_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY2426475.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            32296..32664
                     /gene="rplN"
                     /locus_tag="JZ382_000263"
     CDS             32296..32664
                     /gene="rplN"
                     /locus_tag="JZ382_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY2426476.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32701..33009
                     /gene="rplX"
                     /locus_tag="JZ382_000264"
     CDS             32701..33009
                     /gene="rplX"
                     /locus_tag="JZ382_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY2426477.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            33037..33576
                     /gene="rplE"
                     /locus_tag="JZ382_000265"
     CDS             33037..33576
                     /gene="rplE"
                     /locus_tag="JZ382_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY2426478.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33595..33780
                     /locus_tag="JZ382_000266"
     CDS             33595..33780
                     /locus_tag="JZ382_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAY2426479.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33816..34214
                     /gene="rpsH"
                     /locus_tag="JZ382_000267"
     CDS             33816..34214
                     /gene="rpsH"
                     /locus_tag="JZ382_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY2426480.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            34246..34782
                     /gene="rplF"
                     /locus_tag="JZ382_000268"
     CDS             34246..34782
                     /gene="rplF"
                     /locus_tag="JZ382_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY2426481.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34943..35299
                     /gene="rplR"
                     /locus_tag="JZ382_000269"
     CDS             34943..35299
                     /gene="rplR"
                     /locus_tag="JZ382_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY2426482.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            35320..35820
                     /gene="rpsE"
                     /locus_tag="JZ382_000270"
     CDS             35320..35820
                     /gene="rpsE"
                     /locus_tag="JZ382_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY2426483.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35835..36014
                     /gene="rpmD"
                     /locus_tag="JZ382_000271"
     CDS             35835..36014
                     /gene="rpmD"
                     /locus_tag="JZ382_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY2426484.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            36058..36498
                     /gene="rplO"
                     /locus_tag="JZ382_000272"
     CDS             36058..36498
                     /gene="rplO"
                     /locus_tag="JZ382_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY2426485.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            36498..37793
                     /gene="secY"
                     /locus_tag="JZ382_000273"
     CDS             36498..37793
                     /gene="secY"
                     /locus_tag="JZ382_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY2426486.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37855..38502
                     /locus_tag="JZ382_000274"
     CDS             37855..38502
                     /locus_tag="JZ382_000274"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY2426487.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38693..38911
                     /gene="infA"
                     /locus_tag="JZ382_000275"
     CDS             38693..38911
                     /gene="infA"
                     /locus_tag="JZ382_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY2426488.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38944..39060
                     /gene="rpmJ"
                     /locus_tag="JZ382_000276"
     CDS             38944..39060
                     /gene="rpmJ"
                     /locus_tag="JZ382_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY2426489.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            39078..39443
                     /gene="rpsM"
                     /locus_tag="JZ382_000277"
     CDS             39078..39443
                     /gene="rpsM"
                     /locus_tag="JZ382_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY2426490.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            39471..39860
                     /gene="rpsK"
                     /locus_tag="JZ382_000278"
     CDS             39471..39860
                     /gene="rpsK"
                     /locus_tag="JZ382_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY2426491.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39940..40878
                     /locus_tag="JZ382_000279"
     CDS             39940..40878
                     /locus_tag="JZ382_000279"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY2426492.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40906..41286
                     /gene="rplQ"
                     /locus_tag="JZ382_000280"
     CDS             40906..41286
                     /gene="rplQ"
                     /locus_tag="JZ382_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY2426493.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41558..42397
                     /locus_tag="JZ382_000281"
     CDS             41558..42397
                     /locus_tag="JZ382_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY2426494.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            42373..43242
                     /locus_tag="JZ382_000282"
     CDS             42373..43242
                     /locus_tag="JZ382_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY2426495.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            43232..44029
                     /locus_tag="JZ382_000283"
     CDS             43232..44029
                     /locus_tag="JZ382_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY2426496.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            44232..44978
                     /gene="truA"
                     /locus_tag="JZ382_000284"
     CDS             44232..44978
                     /gene="truA"
                     /locus_tag="JZ382_000284"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY2426497.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(45311..47980)
                     /locus_tag="JZ382_000285"
     CDS             complement(45311..47980)
                     /locus_tag="JZ382_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAY2426498.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     NVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15488 a         8819 c        10262 g        13462 t
ORIGIN      
        1 gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta
       61 tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag
      121 caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat
      181 gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc
      241 gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt
      301 attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca
      361 gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat
      421 agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt
      481 tatataaata tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta
      541 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
      601 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
      661 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
      721 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
      781 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
      841 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
      901 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
      961 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
     1021 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
     1081 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
     1141 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
     1201 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
     1261 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
     1321 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
     1381 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
     1441 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
     1501 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     1561 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     1621 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     1681 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     1741 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     1801 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     1861 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     1921 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     1981 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     2041 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     2101 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     2161 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     2221 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     2281 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     2341 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     2401 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     2461 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     2521 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     2581 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     2641 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     2701 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     2761 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     2821 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     2881 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     2941 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     3001 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     3061 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     3121 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     3181 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     3241 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     3301 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     3361 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     3421 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     3481 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     3541 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     3601 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     3661 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     3721 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     3781 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     3841 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     3901 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     3961 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     4021 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     4081 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     4141 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     4201 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     4261 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     4321 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     4381 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     4441 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     4501 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     4561 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     4621 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     4681 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     4741 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     4801 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     4861 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     4921 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     4981 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     5041 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     5101 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     5161 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     5221 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     5281 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     5341 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     5401 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     5461 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     5521 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     5581 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     5641 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     5701 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     5761 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     5821 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     5881 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     5941 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     6001 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     6061 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     6121 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     6181 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     6241 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     6301 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     6361 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     6421 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     6481 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     6541 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
     6601 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
     6661 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
     6721 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
     6781 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
     6841 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
     6901 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
     6961 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
     7021 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
     7081 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
     7141 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
     7201 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
     7261 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
     7321 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
     7381 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
     7441 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
     7501 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
     7561 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
     7621 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
     7681 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
     7741 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
     7801 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
     7861 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
     7921 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
     7981 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
     8041 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
     8101 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
     8161 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
     8221 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
     8281 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
     8341 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
     8401 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
     8461 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
     8521 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
     8581 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
     8641 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
     8701 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
     8761 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
     8821 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
     8881 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
     8941 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
     9001 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
     9061 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
     9121 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
     9181 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
     9241 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
     9301 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
     9361 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
     9421 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
     9481 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
     9541 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
     9601 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
     9661 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
     9721 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
     9781 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
     9841 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
     9901 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
     9961 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
    10021 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
    10081 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
    10141 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
    10201 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
    10261 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
    10321 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
    10381 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
    10441 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
    10501 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    10561 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    10621 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    10681 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    10741 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    10801 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    10861 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    10921 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    10981 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    11041 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    11101 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    11161 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    11221 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    11281 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    11341 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    11401 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    11461 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    11521 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    11581 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    11641 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    11701 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    11761 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    11821 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    11881 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    11941 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    12001 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    12061 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    12121 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    12181 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    12241 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    12301 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    12361 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    12421 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    12481 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    12541 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    12601 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    12661 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    12721 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    12781 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    12841 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    12901 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    12961 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    13021 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    13081 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    13141 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    13201 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    13261 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    13321 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    13381 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    13441 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    13501 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    13561 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    13621 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    13681 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    13741 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    13801 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    13861 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    13921 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    13981 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    14041 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    14101 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    14161 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    14221 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    14281 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    14341 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    14401 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    14461 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    14521 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    14581 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    14641 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    14701 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    14761 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    14821 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    14881 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    14941 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    15001 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    15061 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    15121 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    15181 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    15241 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    15301 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    15361 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    15421 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    15481 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    15541 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    15601 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    15661 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    15721 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    15781 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    15841 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    15901 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    15961 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    16021 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    16081 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    16141 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    16201 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    16261 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    16321 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    16381 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    16441 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    16501 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    16561 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    16621 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    16681 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    16741 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    16801 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    16861 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    16921 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    16981 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    17041 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    17101 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    17161 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    17221 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    17281 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    17341 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    17401 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    17461 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    17521 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    17581 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    17641 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    17701 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    17761 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    17821 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    17881 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    17941 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    18001 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    18061 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    18121 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    18181 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    18241 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    18301 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    18361 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    18421 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    18481 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    18541 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    18601 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    18661 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    18721 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    18781 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    18841 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    18901 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    18961 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    19021 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    19081 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    19141 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    19201 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    19261 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    19321 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    19381 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    19441 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    19501 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    19561 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    19621 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    19681 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    19741 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    19801 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    19861 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    19921 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    19981 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    20041 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    20101 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    20161 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    20221 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    20281 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    20341 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    20401 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    20461 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    20521 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    20581 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    20641 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    20701 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    20761 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    20821 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    20881 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    20941 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    21001 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    21061 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    21121 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    21181 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    21241 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    21301 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    21361 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    21421 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    21481 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    21541 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    21601 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    21661 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    21721 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    21781 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    21841 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    21901 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    21961 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    22021 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    22081 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    22141 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    22201 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    22261 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    22321 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    22381 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    22441 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    22501 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    22561 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    22621 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    22681 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    22741 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    22801 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    22861 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    22921 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    22981 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    23041 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    23101 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    23161 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    23221 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    23281 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    23341 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    23401 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
    23461 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    23521 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    23581 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    23641 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    23701 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    23761 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    23821 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    23881 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    23941 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    24001 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    24061 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    24121 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    24181 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    24241 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    24301 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    24361 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    24421 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    24481 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    24541 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    24601 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
    24661 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    24721 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    24781 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    24841 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    24901 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    24961 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    25021 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    25081 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    25141 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    25201 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    25261 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    25321 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    25381 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
    25441 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    25501 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    25561 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    25621 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    25681 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    25741 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    25801 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    25861 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    25921 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    25981 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    26041 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    26101 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    26161 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    26221 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    26281 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    26341 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    26401 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    26461 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    26521 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    26581 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    26641 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    26701 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    26761 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    26821 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    26881 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
    26941 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
    27001 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
    27061 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
    27121 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
    27181 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
    27241 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
    27301 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
    27361 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
    27421 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
    27481 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
    27541 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
    27601 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
    27661 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
    27721 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
    27781 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
    27841 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
    27901 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
    27961 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
    28021 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
    28081 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
    28141 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
    28201 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
    28261 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
    28321 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
    28381 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
    28441 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
    28501 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
    28561 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
    28621 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
    28681 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
    28741 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
    28801 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
    28861 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
    28921 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
    28981 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
    29041 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
    29101 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
    29161 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
    29221 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
    29281 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
    29341 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
    29401 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
    29461 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
    29521 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
    29581 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
    29641 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
    29701 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
    29761 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
    29821 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
    29881 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
    29941 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
    30001 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
    30061 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
    30121 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
    30181 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
    30241 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
    30301 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
    30361 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
    30421 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
    30481 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
    30541 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
    30601 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
    30661 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
    30721 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
    30781 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
    30841 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
    30901 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
    30961 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
    31021 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
    31081 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
    31141 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
    31201 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
    31261 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
    31321 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
    31381 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
    31441 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
    31501 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
    31561 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
    31621 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
    31681 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
    31741 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
    31801 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
    31861 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
    31921 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
    31981 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
    32041 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
    32101 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
    32161 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
    32221 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
    32281 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
    32341 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
    32401 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
    32461 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
    32521 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
    32581 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
    32641 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
    32701 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    32761 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    32821 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    32881 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    32941 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    33001 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    33061 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    33121 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    33181 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    33241 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    33301 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    33361 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    33421 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    33481 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    33541 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    33601 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    33661 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    33721 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    33781 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    33841 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    33901 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    33961 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    34021 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    34081 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    34141 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    34201 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    34261 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    34321 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    34381 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    34441 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    34501 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    34561 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    34621 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    34681 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    34741 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    34801 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    34861 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    34921 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    34981 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    35041 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    35101 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    35161 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    35221 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    35281 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    35341 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    35401 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg
    35461 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    35521 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    35581 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    35641 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    35701 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    35761 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    35821 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    35881 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    35941 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    36001 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    36061 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    36121 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    36181 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    36241 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    36301 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    36361 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    36421 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    36481 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    36541 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    36601 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    36661 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    36721 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    36781 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    36841 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    36901 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    36961 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    37021 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    37081 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    37141 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    37201 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    37261 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    37321 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    37381 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    37441 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    37501 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    37561 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    37621 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    37681 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    37741 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    37801 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    37861 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    37921 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    37981 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    38041 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    38101 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    38161 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    38221 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    38281 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    38341 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    38401 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    38461 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    38521 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    38581 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    38641 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    38701 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    38761 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    38821 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    38881 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    38941 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    39001 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    39061 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    39121 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    39181 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    39241 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    39301 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    39361 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    39421 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    39481 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    39541 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    39601 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    39661 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    39721 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    39781 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    39841 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    39901 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    39961 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    40021 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    40081 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    40141 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    40201 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    40261 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    40321 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    40381 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    40441 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    40501 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    40561 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    40621 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    40681 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    40741 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    40801 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    40861 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    40921 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    40981 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    41041 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    41101 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    41161 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    41221 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    41281 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    41341 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    41401 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    41461 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    41521 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    41581 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    41641 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    41701 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    41761 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tgatttttca
    41821 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    41881 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    41941 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    42001 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    42061 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    42121 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    42181 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    42241 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    42301 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    42361 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    42421 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    42481 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    42541 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    42601 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    42661 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    42721 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    42781 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    42841 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    42901 gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    42961 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    43021 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    43081 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    43141 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    43201 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    43261 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    43321 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    43381 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    43441 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    43501 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    43561 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    43621 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    43681 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    43741 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    43801 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    43861 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    43921 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    43981 cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg
    44041 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga
    44101 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    44161 gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag
    44221 gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg
    44281 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    44341 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    44401 atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    44461 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    44521 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    44581 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg
    44641 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    44701 tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg
    44761 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    44821 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    44881 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc
    44941 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca
    45001 gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg
    45061 cttattttcg aagaagctac ttctggaaca ccgtttaaga aagaggtcgt gagcctgtca
    45121 tttaactctg agtcacaaag agcaatttca aaaaacagct tctcatcttt gatgaatatc
    45181 gtgacttgtg gacgaggcgt tgataagtga atcatctttt tgttacctat tttttctgta
    45241 accaaaaaac gatggaacgt tgttttgatt gcccttggtg cggacagtct gaacaggttc
    45301 catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt tcgtggttgt tcttctattg
    45361 ctggatcaat cgtgccataa aaggggcgat tgcggtcaag gtaccagaca ttaagaataa
    45421 acaatgcaat acataggaca aaaaggattg cacctatcgt aaatccttca ggcaagtaac
    45481 tgaaagtgac agtatgttta cctgctggaa cttttatcat caagaatgcg tctttgaaat
    45541 tgctgacctt gactggtttc ccatcaattt tagctttcca tcctttgtca taaggaatag
    45601 ttgtcaccaa catggaatct tcagcggtat ttacagtccc agaagctttt ctgccagtgg
    45661 cttccatttc gacacctttt tctttgatct gattcattgc ggcttgatag gctacaacat
    45721 ctagaccaac gactttaggc tccatgaaac ttatttcttt cgtcccataa aaacttgcag
    45781 taaatgtcac tgttgtctct tgaggataat agccaagatc atagtattga ccagaaatgt
    45841 tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt cgcactagaa ctttctaatt
    45901 gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt atttgcaggc acatggactt
    45961 cccaagtgag ctcttttggc tggttatctg ctgtttctga gtaagtgacg ccagtcgttg
    46021 tctttgtgat cgtcgtattc aactgattct tcaaagtagg tgtatagaat gtaaaatact
    46081 gttcatttag accagacaat gtattgatca agtttgtctg actggtcaaa ttatctgtca
    46141 ctggctgctc aacatcatta acggacaacg gtgcagtaaa tgctagtggc aatgcattga
    46201 cattctgata cagtgtatag tcgccgcttt gaccagcttg gcggaagcca tatttactga
    46261 acgtgctttc gtttttagaa atgttatatt tgattcctgt aaacccatcc atcagcaaag
    46321 tattattgtt ataacggata ttcaaatttg tccctcttga acggaaacct aatttatcca
    46381 ggtaagagga agaattacga tttcttatag aagaaaacaa actgatacca ctgtaaccat
    46441 agttgatcga atcgtttgca gatactggat ccagattttc caagcggtaa aacgagtcgg
    46501 aatctttttt tgtttcattt acaagagctt tcaagtcagg ataaggatca gtatataaac
    46561 tgcgggaagc ataattccaa tcatcaagaa tgccatggat catactgttc gtattgacca
    46621 gtgcttcagc agacatgatc aataataaca atatactcag atgattgaat gaaagttttt
    46681 tatgctgata aaatcccaca gcaaaagcat aaatccctaa gaaaaccact gtcaatacaa
    46741 aggaagtcat cgtcacatag gtgtagcttc cttcaggttt gatgccccat gccaccgcaa
    46801 agagagcagc tacgaccaaa atgattccta acaacagccc accatcgtct tgttctatct
    46861 tttcccatcc ttttcctgca agcaggagca ctaagaaaga gaacagatag gcataacgga
    46921 ataaaaacat attcggtgca tgcataccat gccagaacaa attcaatgga acaagataaa
    46981 aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa tttatttttc aacggtactt
    47041 tccgactggc aaaataaaac agacagagga ctaatggtag cagacctgcg taaacgaacg
    47101 gaatcgatcc gtattttgtc gtgtcataaa caccaatcat atttttcatg attagatcca
    47161 gataagaagt cgcttctgtt ttcaacgtag tgatctgact cagtgcctcg ccatttgacc
    47221 gaagatccag cactgctggc aatacaatga tcattgaagc gccgcctgcc agtaaagaag
    47281 tgataccata aggaacaatc gtttttttat attctgacca gtttgtcaat agacgggcga
    47341 taaaatataa gaaagaaaat acgccaatca taaaacccat ataaaaactg gataaaaaca
    47401 agagtaaata actgataaat aaaagtttcg gccgtttaag atccataaca cgatgaatcc
    47461 ccaaaatcac taatggaaga taaacaaatg catccagcca catgatgatc tccgaatgtg
    47521 cagtgataaa tgagatcaat gcgtaactga cgctaagtgc aacatgcccc cagcgaggaa
    47581 tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc acagccgacc ttaagcaaag
    47641 tcagaacata cagtgcatct ggcatcatct ggtttggaaa aaagaacacc agtggcgtga
    47701 agatcccgcc tagatagtaa gaaattaaag ataaataatt caatcctaga gaagcattcc
    47761 atgtataaaa aatactttgc tttccaagaa gaacatttcg gaagctggca tgaaaattag
    47821 aaaactgtga aaaggcatca cttgccaaaa tacttctgct gcttccagga tagattccga
    47881 tacttagata aacaagtgct aaaatcaaaa acggaatcag gaaactagct gctgcgtagc
    47941 gtccgttttc tctaaagaat gactttaaat tttttttcat atttattttc caccaaattt
    48001 ctatgaagtt gtattcagtt tatcatatcc t
//
LOCUS       DADJYG010000006        47852 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.006, whole genome shotgun sequence.
ACCESSION   DADJYG010000006 DADJYG010000000
VERSION     DADJYG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.006"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(47..346)
                     /locus_tag="JZ382_000286"
     CDS             complement(47..346)
                     /locus_tag="JZ382_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HAY2426499.1"
                     /translation="MKKFFNLANGIFFLSLFSVIFLTLNLQSGKMGVVIFRNLHLPVD
                     ASIVGWLLLLFSYLMSRKHQTKFFARLGGRVALFFLVVYLIFTCLGLLQSLGILP"
     gene            complement(568..2073)
                     /locus_tag="JZ382_000287"
     CDS             complement(568..2073)
                     /locus_tag="JZ382_000287"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAY2426500.1"
                     /translation="MSFSLEDIRHLLLKEHLLKEFVSTKGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMAEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLKETADLFQIPTITYGRSEESN
                     YQVIHSDKGTHGFTLSSRQDHLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQAGLKEARVPGRMDQLIQTNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADVAFLTADDPAFEDPQKIAEEINEAITNPELIVHYEMD
                     RPEAIRRALAESTPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(2165..2755)
                     /locus_tag="JZ382_000288"
     CDS             complement(2165..2755)
                     /locus_tag="JZ382_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2426501.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWIINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMLSSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGWLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2934..4580
                     /locus_tag="JZ382_000289"
     CDS             2934..4580
                     /locus_tag="JZ382_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAY2426502.1"
                     /translation="MDNQRKPALDQLSPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMNGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKVNIATKELLLDTIKEAIPFIIVGSGVTIFKLVDQF
                     TFMRIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENRAAISYLVVGFLVKLLLQYPAIRLFEVYGPLLATMLGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLFASWITKIIFGTFLNEDSKFQSLLL
                     ILAVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4629..5348
                     /locus_tag="JZ382_000290"
     CDS             4629..5348
                     /locus_tag="JZ382_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAY2426503.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKTQVNEF
                     EDTVTYLGEIIAYQKDFYYILHKPAGVISATQDNHDQTVIDLLKDQDYREDLFPVGRL
                     DKDTEGLLILTNDGIFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDCDEQ
                     TLPAKLQIDQVDQESETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGNFWLPK
                     DLNKGQYRPLTKEELEQVREK"
     gene            5827..6774
                     /locus_tag="JZ382_000291"
     CDS             5827..6774
                     /locus_tag="JZ382_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2426504.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVTFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTVARRFDSDSALF"
     gene            6786..7706
                     /locus_tag="JZ382_000292"
     CDS             6786..7706
                     /locus_tag="JZ382_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2426505.1"
                     /translation="MKKKKVERVQVRSFNKTTNFFSNIMIALFAISCILPFIFVIAIS
                     VTDESVLSTHGYSFWPEKFSLFGYTYLFDQMQDKIFQALYVTMLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7741..9201
                     /locus_tag="JZ382_000293"
     CDS             7741..9201
                     /locus_tag="JZ382_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2426506.1"
                     /translation="MKLWKKLAFTGVSALLVGTLAACGGSGSKEQAEASDSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGAKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSEDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIAEAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKEALDKAGYDKVLKEMQKQYDEFRQEQ"
     gene            9455..10069
                     /locus_tag="JZ382_000294"
     CDS             9455..10069
                     /locus_tag="JZ382_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAY2426507.1"
                     /translation="MLGKVLEELFIRIWVVIKLTLYFWIYTFVGGIIFGLGTAWKTVN
                     ELFYLYGFEYKEITLKRGWNIYKRNFLRGNLLFGLFLSSTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALFCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKTIIGSG
                     IIIAVTWQFKGLILFGVIGLLTVWNGTTTKQWREELDKQLESYE"
     gene            10062..11786
                     /locus_tag="JZ382_000295"
     CDS             10062..11786
                     /locus_tag="JZ382_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2426508.1"
                     /translation="MNRIRILFSKGTLLNRLLRKYAVIMIGVTMTATIIFSVHTWDQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGDSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYINYEELSAVYVVLNQLPEYYESSRENKGGEVKAGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDTILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFGRQETIISESLQKNSQLPIANLRKNNFIQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIVGIIVLDSILLIFLYKTFKRYSQQISIVMQAMEQTAAGNLETRIDT
                     TKTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEDKRVRIIVKD
                     NGRGMTEKRLKQVEEKLSHPKVELHESIGLQNVNERLRANFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11789..13234
                     /locus_tag="JZ382_000296"
     CDS             11789..13234
                     /locus_tag="JZ382_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426509.1"
                     /translation="MYQVLLVDDEYMILNGLKKIIDWKSLGFQIVATAENAKEGLSVL
                     EQQRIDLVVTDVTMPEINGLEFIEAAQKEHHNFEFMILSGYQEFDYLKEGMQLGAVNY
                     LMKPVNKFELIESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEASEEEIIGQ
                     ISEKQHRIVLIQLSRIHDPLINTWLKEHQQIFYYQRNYGDRILITLLLEADEVDRFCC
                     FVQENIPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAIQATIK
                     EQTIDFSSFRRVLQNKRLDDAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRELI
                     DLEDEEYLQGIEKIHQAKTVQELHQLLLSFVQKHQRQKKYSGNVEKVIEILHQHYQEP
                     LTLKEVSESLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNVNEIASE
                     IGYNNTTYFSKLFKKIVGRSPKEYREKRGES"
     gene            complement(13375..15528)
                     /locus_tag="JZ382_000297"
     CDS             complement(13375..15528)
                     /locus_tag="JZ382_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY2426510.1"
                     /translation="MNIGQIDTRHGTANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPEDRVFQGYRLTHQPSPWMGDFSHMLLTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSIRQLRYGIRSTLIPSMYGGILSIDYSRNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQIIRFGTS
                     FISSEQAAYNLELEQNQSKETYLANSQKQWQSYFDRIQLTHHDQQQLRTFYHNFYRVF
                     LFPQTFYEMDPSGQKIHYDTTSRSVKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRPDLMQEFLTAMKKG
                     ATVQSNHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLAEDETV
                     KKYQQRSLNYQNIFDYETGWMRSKDKNGKFREPFSAHRWGQDYAEGSAWQSSFAVYHD
                     FAGLIEKYGGSERFKEKLIDLCNQSPTFETGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFNDSSTGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGIGEYVIGMPLMDKAVVLLSNGKELTIEATPNRPQQQFIHKINRMDKEYTKLYFTH
                     QDLLKGGKISYQLGIVPHPKKYRSKDLPFSLSNDN"
     gene            complement(15530..16576)
                     /locus_tag="JZ382_000298"
     CDS             complement(15530..16576)
                     /locus_tag="JZ382_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY2426511.1"
                     /translation="MNQPLYQTIFNDLQMAILTGELPVDSQLPTEKELSHTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQMLPSPTKTRRILFLLPYVADLSVGDFT
                     KGLNPVMQDKQMDVMMTTHDFLQNKTAKELIHEFDGLIYYAFDTEQHLDLLFELSLKE
                     FPVIILDKKIYELPFPTVLSDNFQGGVLATSHLIEQGHSKIAYLFGAQTHPQSVRQRY
                     LGYLEALDKAGLAFHTSLDDKSATFDELLSYITKYEVTAFVCENDVVAIQTMNFLRQH
                     DYHVPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGEALITWLETNERPSDSK
                     FPVSFIKRQSTKEK"
     gene            16726..18015
                     /locus_tag="JZ382_000299"
     CDS             16726..18015
                     /locus_tag="JZ382_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAY2426512.1"
                     /translation="MVYKDIPKSVQKFMNDITQKCGKNHADWATNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNP
                     TFIEGIKKILGVFETEQDHEHSPYTFIRDTTRKEDTLINDGRGAKTTFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQKAQILKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGHATVMDDSNVPNLIAAPYLGYCSEKEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPKEYVWPIALAMEGMTTPDKSEKERLLNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            18028..20721
                     /locus_tag="JZ382_000300"
     CDS             18028..20721
                     /locus_tag="JZ382_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY2426513.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQALQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MMIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTEIFGLLFANWYSNGNEIPVEKEAARVFWKQKLADAEQY
                     ASTDHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVRQELPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVTGIYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLATAID
                     TTGFPKDSFPFVVVNTAGMDKTGEAEITIELERKRFAEGIPEHLYQELEQLPRRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARTIKVTLPIKNMPAFSWETFALVE
                     GEAKAKAEEKETMIRQSGRIVENAHLRLTIDKNGAITMEDRKNKRKWNNLHIFEDIGD
                     IGNEYIFKQPVYDQPILSSNQEDSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQ
                     RMVVEFRYRKAERSKEKRILQIETIMTVRKDSKKIDFETTLDNQMKDHRLRILFPTKL
                     HVEHHEADSIFEVVKRPNHVSRSWENPTNPQHQQAFVNIHDEQNGVTVGNFGLNEYEV
                     TADGQIAVTLLRCVGELGDWGYFPTPEAQCLGKHQFNYSVELHGPEETFSTYLHAYAA
                     QIPFSTQQIKHHEGTLASKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKL
                     EITKDNRKTVILNLLEEPTQQSVVPIIQPYEIRTIGFEEEN"
     gene            20722..21579
                     /locus_tag="JZ382_000301"
     CDS             20722..21579
                     /locus_tag="JZ382_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2426514.1"
                     /translation="MYGGIEAGGTKFVCAVSEDGEILEKTSIPTTTPEETLDQVFEFF
                     DRYELNGIGIGSFGPIGIDQTKDNYGYVLATPKKGWAQFDFLGTIRKRYNVPVAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DQYEGTCPYHKDCLEGLAAGPSIEARIGIKGQDLPQDHPIWDIQAFYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21646..22503)
                     /locus_tag="JZ382_000302"
     CDS             complement(21646..22503)
                     /locus_tag="JZ382_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAY2426515.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKKQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGKIAKDRTITNKIGRDRHDRRKRVVDDKNGQNALTHVTVDKVLSGKT
                     QVYCVLETGRTHQIRVHLAHIGHPIVGDPLYQNRPAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWK"
     gene            22714..24885
                     /locus_tag="JZ382_000303"
     CDS             22714..24885
                     /locus_tag="JZ382_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAY2426516.1"
                     /translation="MDFQSILGKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPANANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLHDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNNALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSVLKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPMIMAGAYS
                     AFANEGQMYTPHLITKIVDSTGAVIIDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24953..25294
                     /locus_tag="JZ382_000304"
     CDS             24953..25294
                     /locus_tag="JZ382_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAY2426517.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25568..27322)
                     /locus_tag="JZ382_000305"
     CDS             complement(25568..27322)
                     /locus_tag="JZ382_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426518.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMTCLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYRSPVVFLFFLLTVPLAALVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFENVSFHYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPSSGRIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLNEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEMG
                     TYEELLAQEGYFYRLKNE"
     gene            complement(27333..28169)
                     /locus_tag="JZ382_000306"
     CDS             complement(27333..28169)
                     /locus_tag="JZ382_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY2426519.1"
                     /translation="MFPLNLGIRAHDLEVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLNAMTLGSAAYFGKYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSVIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLVDEVASPNLKIILDCANLMHKENHTDQEKVIQGALDNLQGCITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28171..28854)
                     /locus_tag="JZ382_000307"
     CDS             complement(28171..28854)
                     /locus_tag="JZ382_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAY2426520.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKTFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVHMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKYHIPLLDVFSRSQ
                     EYFQTFSKEETKKFYLHLMPNSCENYPEGLKDNTHFSPEGADKVARIIAELIKESRLP
                     LADYLQKGV"
     gene            complement(29013..30407)
                     /gene="uxaC"
                     /locus_tag="JZ382_000308"
     CDS             complement(29013..30407)
                     /gene="uxaC"
                     /locus_tag="JZ382_000308"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY2426521.1"
                     /translation="MFLSDDFLLKDDWAKNLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFDIDLLINEENAEKIWTQANEILASPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNVWITLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDTLRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IRLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETNQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30446..31945)
                     /locus_tag="JZ382_000309"
     CDS             complement(30446..31945)
                     /locus_tag="JZ382_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY2426522.1"
                     /translation="MLETLQPTMKLNQRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALSDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYPFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAVENDQRIPVPLSTLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEKVFGSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31938..33359)
                     /locus_tag="JZ382_000310"
     CDS             complement(31938..33359)
                     /locus_tag="JZ382_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY2426523.1"
                     /translation="MNKLTKELVKKNEAPVKVLQFGEGNFMRGFVDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITIVEQMINPYHEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDERTDTPPQSFPGKLTAFLYRRFELNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFIEWLEKENVFCCSLVDRIVPGYPRED
                     ADVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLRFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFDLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEKNQVPPLISVSLAALL
                     LMYRGDQVKPHDDEKTISEFVDAWSDNETAILRLLQNAALWGEDLSQLPNVADTVQEM
                     ATKIDQKGVRAVLQEMKGTMVHA"
     gene            complement(33420..34412)
                     /locus_tag="JZ382_000311"
     CDS             complement(33420..34412)
                     /locus_tag="JZ382_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2426524.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YLPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDA
                     VDLTEINLQRYDGLIVMSQSDEDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLDSLKKASIPANPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHSAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVHKPLKEMCQVGTKLLLDLIEDRPIEQSKMELSTLLMLRESINYL"
     gene            34643..36841
                     /locus_tag="JZ382_000312"
     CDS             34643..36841
                     /locus_tag="JZ382_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2426525.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTNYSTYSEIRKELNFI
                     ETIVMDDNNYDLLSLAGKWGVINGSLTQLTEEEVERYQQKYINNKDNLFWQKKNKGIE
                     MVIPLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEDGKLFSTQKVKEEEQP
                     EALADVDLEYNQLKVIRENGTSYIVLKSDYNNWIYRLEVDSSAIGATIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKEKRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLKLTVSIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPVEEQNRLFEAIRSGNQEVRKLVHELINALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37194..38066)
                     /locus_tag="JZ382_000313"
     CDS             complement(37194..38066)
                     /locus_tag="JZ382_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2426526.1"
                     /translation="MKKRVSIGERSFTFFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIAYPFVQ
                     KYFVKGALVGSVKG"
     gene            38364..39338
                     /locus_tag="JZ382_000314"
     CDS             38364..39338
                     /locus_tag="JZ382_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2426527.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKSGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39360..40052
                     /locus_tag="JZ382_000315"
     CDS             39360..40052
                     /locus_tag="JZ382_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAY2426528.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRTKETFPLTKTYFSYFKENYRETLLMSWIYGVIGVVLLVDLSFIHNWYL
                     RIALYIAAFLYLLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSLVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40018..41523
                     /locus_tag="JZ382_000316"
     CDS             40018..41523
                     /locus_tag="JZ382_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2426529.1"
                     /translation="MKKATLVKLGLSFAGILALAACGNSSNNSSSETEDGKTKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRSALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPMQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGKTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDMYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDADPEKELSNQLIKENEEFAVMDPSVPLDSATNNEVG
                     SEIEKIVVDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAQ"
     gene            41597..42700
                     /locus_tag="JZ382_000317"
     CDS             41597..42700
                     /locus_tag="JZ382_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAY2426530.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     EQTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPETGLSHHFWSRSIGWYLMAAADT
                     YELLHAETDCSLLKEILEKTLTALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGAEQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42703..44040
                     /locus_tag="JZ382_000318"
     CDS             42703..44040
                     /locus_tag="JZ382_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAY2426531.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASAGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPIVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKTAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSKQGFYIGFA
                     DKVTFNQMTIENHEGPAFLLENTKEISIEKFRSTQSENMDELVVRKNFAYAVE"
     gene            44027..44905
                     /locus_tag="JZ382_000319"
     CDS             44027..44905
                     /locus_tag="JZ382_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAY2426532.1"
                     /translation="MQLNDFLGDPSSRLTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRIATIG
                     LSMGGLLSWWVAALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIKEHL"
     gene            44902..45825
                     /locus_tag="JZ382_000320"
     CDS             44902..45825
                     /locus_tag="JZ382_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAY2426533.1"
                     /translation="MRMKDKLIIGKNSIDTPTDFETIQEGIDYLADLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGTGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVLLKNCRLTGYQDTLCTGPLPDLQKDGTPFC
                     TPASVNPDYCRQTYEYCWIEGTVDFIFGGADAIFRQCRIHSLASKNPGYITAASTPKK
                     QKSGYRFESCTLTAEPDTRNVYLGRPWRPYANVVFEACKIGGHVHPTGWDNWDDKKNE
                     QTAKFSERNNNYETPIKRPEWIHLEKQGVLK"
     gene            45822..46232
                     /locus_tag="JZ382_000321"
     CDS             45822..46232
                     /locus_tag="JZ382_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAY2426534.1"
                     /translation="MKRKWELLLGMIGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHPSLSLENTFELLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGV
                     ILLVGTQFIAFPFAFFYFAAGAFSLYRVRIRKEA"
     gene            46236..47804
                     /locus_tag="JZ382_000322"
     CDS             46236..47804
                     /locus_tag="JZ382_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAY2426535.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRAKDRYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTSDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNIIGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGKWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTAPMEEIMIETNEEVQQERAYQFMDTFEGELKK
                     NWNARRILPRADWCDTKTRPGFLRLIAGESLQSTFGHHLLAIRQTDFTFDAETAFEYT
                     PNNFNQMAGLVLYLNESNYIYCYVTWDENKGKCLRMIESENGVAQIEEWRIPLTEKKT
                     FLKVAVKKETAQFYLKDTPSEEWKEVGLQKNMCILSGGFTGNFIGIVVHDLNRKRGSY
                     ADFEFFKYDGKDHE"
BASE COUNT        15415 a         8593 c         9847 g        13997 t
ORIGIN      
        1 tgtcccagcc tctatttatt atagtcaata tgattcgaac gaagtatcaa ggcagaatac
       61 cgagcgactg aagcaaacct agacatgtga atatcagata aacgactaga aaaaatagag
      121 caacccttcc gcctaagcgc gcgaaaaact tagtctgatg cttccgactc atcaagtaag
      181 aaaaaagtag cagtaaccat ccaacaatcg atgcatcaac tggcaaatgg agatttctaa
      241 aaataacgac ccccattttg ccactttgca aattcaaagt tagaaaaatg acagaaaaaa
      301 gagaaagaaa aaagataccg tttgctaagt tgaaaaattt tttcataata accaccttta
      361 tattattgaa aaaaaggaat aacaaactaa aagcaacgat agaagaacca gtaagcaaaa
      421 cggatgatag cgctatcttt aagaggcatg ataaagaaac aataaaaatt aacgcagttc
      481 cggcaacttt tcttgataat tccatagacg ggacaccatg aaaagttttc ttttcatagc
      541 gtcccgtcca ctagaacata aataacatta ctcttcaatc aaccgcttca cgattgcata
      601 atccccttca taaggttcat ctacaccatt gattttttga tagggatcaa ccccttttcc
      661 cgcaatgacg actgaatcct gcggagtact ttctgctaaa gcacgtcgaa tcgcttcagg
      721 acggtccatt tcataatgaa cgatcaattc tggattagta attgcttcat tgatttcttc
      781 cgcgattttt tgcggatctt caaatgctgg gtcatctgcc gtcaaaaagg caacgtcagc
      841 tgtttctgat agcacttttc caaaatcatg tcgacgggag attgctttgt ttcctgggct
      901 gcctaataca acaattacac gaccatctgg atgttcattt ttggcaaatt ctagcaatgt
      961 tttcaaactt agataattat gtgcgtagtc cacatagaca tgcgccccat ttgtttggat
     1021 caattgatcc attcgacctg gtacacgagc ttcttttaat ccggcttgtg catcttgact
     1081 ggaagcaccg gttagtgctg cggcaatgat cgcactggct gcattttctt gattgaatcc
     1141 tccggccagc aaaatatcat aagcagtatc gcagatagct aagtgatctt gacgagaaga
     1201 taaagtaaac ccgtgggtac ctttgtctga atggatcact tgataattgc tttcttccga
     1261 acggccataa gtgatcgttg ggatctggaa tagatcggcg gtttctttaa gcaaatcaaa
     1321 atagtcactt tcgtgattca aaacaactgt ttttgagttt aagatcaatt gacgcttgca
     1381 atagaaataa tcatcgaatg ttggatgttc gatcggactg atatggtcag gcgagatatt
     1441 caagaagatt cctacatcta aagtcaaccc gtacacacgt tgtgttttgt aagcttgaga
     1501 agaaacttcc atcactagat gagtcatacc attttcaact gcttctgcca tcatacgata
     1561 taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa tacgttttgc catctagcgt
     1621 tgtattcatc gtagaaagca atgcagtttt tttattcgtt gtgtgatcca agattgcttt
     1681 agtaaagtac gcagcagttg ttttaccttt tgttccagtg aatccaatca ctttcaattt
     1741 attttgtgga tagtcgtaaa aggccatagc aagaagtgcc attgctttac ggatatccgt
     1801 cacaatgatt cctactgctg aattttcgta tggctgctct gatacgtaat attggatacc
     1861 ttgttcgatc gctgaatcca aatattcttc tttgaaattc attcctttac agaaaaacag
     1921 agtagaagca tcagcttttc ttgaatcata acttaactga agaaattcag ctttttctgg
     1981 tacatcaaga tgccagcctt ttgttgaaac aaattctttt aatagatgtt cttttaatag
     2041 taaatgacgg atgtcttcta aagagaagct cattgattga tccctcgctt cattcaattt
     2101 cttgatttat tatagcacac tcttttcaat atatcgtttg attccgctat gacaaaatcc
     2161 agctttattc aaattttctt gacacagggg acaagcggat gagtattttc ataaacaatg
     2221 aactgactgt aatagatagg agaacgaaca gtgtctctaa ccatccaaat acataataat
     2281 tcaaaaagac gataaggcta ttcgtaagta acagtccttg cggcattgga agccgaaaaa
     2341 atttgtggaa aataatcggc ggtatcgtta tcccgccatt tgaggctcca acggtgtaca
     2401 atgtgtaaac cccaagactg aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg
     2461 agagcatttc cacttttgga acaatctcta aaacaacagg aaaaagcaga cttcccgcta
     2521 cagttttaaa aaatactgat tttcctaata gaacagcggc tagaagcagc atcataaggt
     2581 tgatgatcca aacagtaact gagcgctgga tagaaaaggc cgtttcaact aaatgtccga
     2641 ttgcgcccac tccggcggaa gctatatcat gagggagcaa gaaccaattc aatccaactg
     2701 tgataaagaa aagacctaaa aatataacgg gtagtttttt tatttcttgt gtcattgata
     2761 tctcctataa atgttagcca taaaaggctg tatatttctt tactttacca aaaaaacgaa
     2821 atcgaattca aaaaaacaat cgttcttata aaagttatat aaaatcgatt gccagtttct
     2881 gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt tggaatctga ccgatggata
     2941 atcaaagaaa accagcatta gatcaattat caccgcaaga aaaaatggca cgaggctctg
     3001 cctggatgac cgccagtaat atcatttcaa ggttgctagg agctgtctat atcattcctt
     3061 ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg attattcaat atggggtaca
     3121 atatatatgc attgtttcta ctgatctcta cagcaggaat acccgcggcg atcgcaaaac
     3181 aaaccgcgcg atataattcg ttgaatgaat atggcacttc acgacgatta ttcatccggg
     3241 cattacagat gatgggcggg ttaggtctgt tgttcgcttt gttcatgtac atcgcctctc
     3301 cttggctagc tcatgcttct ggtggcggag aagaactgat tccaacgatg cgctctttga
     3361 gtatcgctgt attggttttt ccttgtatga gtgtgatacg agggtatttt caagggaatc
     3421 aagagatgat gccttatgcg ctgtcgcaga tcgttgaaca agtggcacgt attttttata
     3481 tgctattggc tacattcatc atcatgaaag tcatgaatgg cgattatgtg acagcggtca
     3541 cgcaatcaac tttcgcggca tttatcggta tgctagcaag tatccttgtc cttttgtatt
     3601 ttttgaagaa acatcaagcg tatacatctg catttatcca ctacagcgaa aacaaagtaa
     3661 atattgcaac gaaggaattg ttgttagata ccattaaaga agcaataccg tttattatcg
     3721 ttggttcagg ggtaacaatc ttcaaattag tcgatcaatt cacttttatg aggatcatgt
     3781 ccgcatccac tgaatattcc aacgcacagt tgttagatct tttttcgatt ttcagtgcca
     3841 atccagataa gttgacgatg gtagttatcg cattggcaac atcgatcgcg tctaccgggt
     3901 taccgttgat tacagaagct gttacgttga aagatcgagt agggctggca aaattgacga
     3961 gcggaaattt acaactgttt tcatttttta tgtttccagc gacattcggt gtcatgttac
     4021 tcgcctatcc gctaaacaca ttattttata caccagattc tctaggaagc aatgtactga
     4081 tccaagcaag ttttgtagga ctcttcttag ggttatatat gctagtttcc aacatgctgc
     4141 aaggaatgtt tgaaaacagg gcagcaatca gttatttggt tgttgggttt ctggtgaaac
     4201 tacttcttca atatccagcc attcgtttat ttgaagtgta tggcccttta ttagcgacaa
     4261 tgctcgggtt tgctgtgtct tgtaccttaa tcttaaagag gatccatgaa gtagcccgct
     4321 ttaaaccaag ttttgtctgg cgtaggacat tactgatctt tattcttacc ttgattatgc
     4381 tttttgcttc gtggatcaca aagataattt ttgggacttt cttaaatgaa gatagcaaat
     4441 tccagtctct attattgatt cttgcagtag caggtatagg ggcattggtg tacggttatt
     4501 tagctttgaa aatccgtttg gcagaaagac tactaggacc aggaatagcg cgtttacgac
     4561 acaaattaaa aataaaataa gtgaatggga agagcagaat caatgctctt ttgaaatggg
     4621 ggaaacgcat gcgtttagat aaatttttag cagatatggg acttggtagc cgcaaagaag
     4681 tcaaaggatt gatcaaaaaa ggaacgattg aagtcaatgg gcaagtggtc aaaagcgata
     4741 aaacgcaagt aaacgagttt gaagatacgg ttacttattt aggagaaatc attgcgtatc
     4801 aaaaagattt ttattatatt ttgcataaac ctgcaggagt catttctgct actcaggata
     4861 atcatgacca aaccgtaatc gatttgttga aagatcaaga ttatcgggaa gatctttttc
     4921 cagttggccg attggataaa gacacggaag gcctcctgat tttaacgaat gatgggatat
     4981 ttgctcatca gttattatca ccaaaaaaac acgtggagaa agaatatcta gctgaggtta
     5041 gcggtataat gacagaagaa gatatccgtt tatttgcaga aggattaatt attgattgcg
     5101 atgagcagac attgccggca aaactccaaa ttgatcaagt agatcaagag tcagaaactt
     5161 caattatccg actgattttg catgaaggga agtttcacca agtcaagcga atggtcaaag
     5221 cagtaggaaa agaagtctta tatttaaaaa gaatccgtat ggggaatttt tggctgccta
     5281 aagatttgaa taaagggcag tatcgccccc tgaccaaaga agagctagaa caagtgagag
     5341 aaaaataagt gaatggacag ttaaaagaat aagtagcgta gttgtagcgc catctgaaaa
     5401 gcagacttat ccgtctgact tttggatggc ctttttgtga aatttcgact tatttccgta
     5461 gaagctactt ctggaacatt gtttgttcag aataagggac tgtgacaaga cttttgtcac
     5521 agtccccatt gctccttgtg actcggagtt agatagtaag gtcacgatct ccctaatatt
     5581 caggacaaaa cgctgatttc acaacctctg ataaacggtg tgcaatcagc tgaccctcgg
     5641 tattagctca aaaaacggca aaatatgaga aactattttt ccgttctttt ctctaatact
     5701 caggacaaaa cgctgatttc acaacctctc ctatactttg tttcattttc cctatacttt
     5761 gtttatttat tctcattatt gaaagcgcta taattgcgtt ggaaatgaat cgaaaggggt
     5821 ttttagatga aaaaacgaaa ggggttcaat cattttttta tgaatctatg gcgttataaa
     5881 gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt ttacatacct
     5941 gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg gttcctggct
     6001 agcctgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt ttcttcggac
     6061 caagcatttc tcatcactaa aaatacaatt ttatataatg tgacatttat ccttttaaat
     6121 ctgctcattt cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa acgacttgtt
     6181 aaagtttatc aaacgatgtc cttgctccct tattttctat cttgggtcat tatcagttac
     6241 ttcgtttacg cctttttaag tcccgacaaa ggaatattca accagtggat tactggacac
     6301 ggaggagaag ctgtcaattg gtacaatgaa ccgaaatatt ggccatttat cttagtgttc
     6361 atcggtacat ggaaagggat tggttataac agcatcattt actttgcatc tgttatggga
     6421 atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg gcagcaaatc
     6481 aaacatgtga cgataccaca actgattcca ttgatgacga ttttaacgat tttagccgta
     6541 ggaaacattt ttcgagctga ttttggcctg ttttataaca ttcctaggaa ttccggtgca
     6601 ttgtacgatg tgactcaagt tttggatacg tatatctaca atgggctgac atccacagga
     6661 gattttggta tgacggctgc tgcaggactt tatcaatcag ttgttggttt tgtcttattg
     6721 atgatcacaa atacggtcgc ccgtcgtttt gatagcgatt ctgcattatt ctaaggagga
     6781 aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ttctttcaat aagacaacca
     6841 attttttctc taatataatg attgcactct ttgctatttc ctgtattttg ccatttatat
     6901 ttgttattgc catctctgtt accgatgagt ccgtattgtc gacgcacggg tacagttttt
     6961 ggccagaaaa attcagtcta tttggttata cgtatttgtt cgaccagatg caggataaaa
     7021 ttttccaagc gctttatgtt actatgcttg tcactgtttt aggtacactg atcaatagca
     7081 cggctacttc tttatatgct tatgcgattt cgcggtcaaa ttttccattc cgtcgttttt
     7141 ttaccgtttt ttgtttgatt acgatgttgt tctcacccgg tatggtagct aattatcttg
     7201 ttatgacaaa tcttctccag ttaaaagata cgatttgggc attgatcttg ccaatggctg
     7261 tcagcccctt caatattatt gtgatgcgca cattctttaa acgttcggtc tctgattcga
     7321 ttattgaatc ggcgcggatc gacggagcta gcgaacttcg aatattcgtt caaatcgttt
     7381 taccgttagc tgttcctggt atcgcaacaa tcagcttatt tgccgcattg ggttactgga
     7441 atgactggtt caatgcttta ctatacattc aagatgataa gctagttcca ctacagtatc
     7501 ttttgatgaa aatccaaagt aatattcaat atctgacgca aaattcagga gcaggaagcc
     7561 aattgacagg tgggctagct tcagttccag gagaatccgc cagaatggcg atcgtagtta
     7621 tctctacgtt gcctattgcg atcagctatc catttttcca gaaacatttt gtcaaaggtc
     7681 taacgatcgg tggagtaaaa gaataaaaaa atataaatca taaaattagg aggaagaaaa
     7741 atgaagttat ggaagaaact agcctttaca ggtgtttcag cattattggt aggtacatta
     7801 gcggcatgcg gaggttctgg ttctaaagag caagcagaag ctagtgattc ggacactttg
     7861 cagatgtatc aaatcggcga taaaccggat aatttcgatc aattgatgga agtggcaaac
     7921 aaacgaatcg aaaaagagat cggtgcaaaa gtcaatatca attacattgg ttggggcgat
     7981 tacgaaaaga aaatgaacgt catcatttct tccggagaaa attacgatat cgcctttgcg
     8041 aacaactatg tttccaatgc tcaaaaagga gcctttgctg atttgactga attagcgcct
     8101 aagtacgctg aaaaagcgta taatgattta gatgaagctt atatcaaagg aaatttggtg
     8161 aatgggaaat tatatgcctt tccagtaaat ggaaatgtct ttgctcaaca agtacttact
     8221 tttaacaaag ctttactaga taaatatgat ctttcaattg atggaatcca atcttacgct
     8281 gatgcagaaa aagttttgaa ggaattccat gaaaaagaac caaacacggc cgcttttgcc
     8341 attggacaag gattcaaagt ccaaggaaac tttgactatc ctttagggaa cactttacca
     8401 tttgctattg atctggatgg agatgattca aaaatcatca atcaatacga caatgaaacg
     8461 tatatcggcc tgttgcgtac gatgcatagc tggtacaaac aaggactcat tccaagtgac
     8521 gctgcaacga gcaataagga ctatccacta gaaggaaata cttggctgat gcgtggagaa
     8581 acacaaggac cctatgatta cggcgatacg attttgacaa atgcagcaaa acaagaacta
     8641 gtttcaaaag caattactgt tccattgaaa tctacagctc aagcacaaat ggcaaatttt
     8701 gtcgtatcca atacttcaaa aaataaagaa aaatcagtag aacttctagg gctgcttaac
     8761 agtgatccag aattattgaa tggacttgtt tggggaatcg aaggagaagc atgggaaaaa
     8821 gtcgatggtt cagaggataa aatcaaattg ctagatggtt atcagccaaa cacacatatg
     8881 tctgcttgga atacaggaaa caacaagatt ctatatacac aagagtccat tacggatgac
     8941 atgattgcca aacgcgacca atcgattgct gaagcagaaa cttcaccgat tttaggattc
     9001 agcttcaaca cggacagtgt gaaaacagaa ttaagtaata tttcaaatgt catgaaccag
     9061 tatttggatg gattgaatac aggaactgtt gatccagatg aaacattgcc gaaattaaaa
     9121 gaagctttag ataaagctgg ttatgacaag gtattgaaag aaatgcaaaa acaatatgac
     9181 gagtttcgac aagaacaata atgaataaag acaaagaggt tgtgacaaaa acgtgtcgca
     9241 acctcttgtt ccgaataaac ggtgttcaaa accttgcatc tgcggcaatc agcccaaccg
     9301 aacgaaaaac gtagagagct atttttgttc ggctattctt attacttgat gcagggcagc
     9361 tcttttacaa cctttaatac tcaggccatg gtgctgattg cccaaccttt ttgtataatg
     9421 agaaagtata aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt
     9481 gtttatccga atctgggtcg ttataaaact aaccttatat ttctggatat acacgtttgt
     9541 cggcggaatc atctttggat taggtacggc atggaaaaca gtcaatgagt tattttattt
     9601 atatggattc gagtataaag aaatcacgtt aaaaagaggc tggaatatat ataaaagaaa
     9661 ttttttaaga ggaaatctgc tatttggttt atttttatct agtactgctt tattgagcta
     9721 caatctatat ctatctgttc aaatcaaagg actcattttt ttagctattg attttatttt
     9781 gctttttgcc ttattttgtc tatttgctac ttatcaatac agtatgattt tagacagtga
     9841 atatacgatc tctgttccta atctactaaa gctgagtttt atttcggttt tttccagctt
     9901 ttcgtctttt ctaaaaacaa ttattggtag cggtataatt atagctgtga cttggcaatt
     9961 caaaggattg atcttatttg gtgtcattgg tcttttgacg gtttggaatg gtacgacgac
    10021 gaaacagtgg cgtgaagagt tagataaaca gttggaaagt tatgaatagg atccggattc
    10081 tattttcaaa aggaacgtta ctgaatcggc tgttgcggaa atatgcggtt atcatgattg
    10141 gtgtaacgat gactgctact attattttta gtgtccatac gtgggatcaa aaccaaaagc
    10201 aagcagaaaa catgacaagt gatgccgttc aaagtacaag cagaatgctg aatgacaaaa
    10261 caactttgag taggatcatt aaaaaccaac tcgtcggtga ttccgaaaaa atcgaaaatg
    10321 tcactaccta cttaacaaaa cccatagatc agtatttgat gtatgtatat gagcagcaaa
    10381 acagtaccga cgaattagtt tctttcccaa atcaaatcaa agatctttat ataaattatg
    10441 aagaactaag tgcggtatat gtcgtactga atcagctacc ggagtattat gaatcttcta
    10501 gagaaaacaa aggcggagaa gtaaaagcag gaacgccaaa attactagac gccttttata
    10561 tcaagttgcc tatcacacaa ggaggagctg agtcaggcag tatttttatc ggtttccaaa
    10621 agaaagagct agatacgatt ttagagaatc taacgtcatt caatggactc tctttataca
    10681 tgatttctgg cacgacaaat cgcttgtata cgttccatga taataaaata gctaaagaaa
    10741 cgtttggaag acaagaaacg attatctcag agagtcttca gaaaaattca caattgccaa
    10801 ttgctaatct gagaaaaaat aattttatcc aacatcaaga actgtcggat gattaccgaa
    10861 tattagctgt attagataaa aaaagcgtaa aaatcgaaac gggaaaaaac cttgccccgc
    10921 tgattgtcgg tatcattgtt ttagacagta tattgcttat ctttctttat aaaacattca
    10981 aacgatattc acagcaaata tccatcgtca tgcaggcaat ggagcaaaca gcagcaggga
    11041 atttagaaac ccggattgat acaacaaaaa cggaatatga actaaaggaa ctttcaatcg
    11101 gtatcaacga aatgttggat agcatcaatc aatttgtcga agacatctac aaactagaaa
    11161 tcaaacagca agatgcacat atgcgggcgc ttcaagcaca gatcaatccg cattttttat
    11221 acaatacatt agaatatatc cgaatgtatg caatcagcga aggaagcgaa gaattagcag
    11281 atgttgtata tgctttttct gccttgctcc gaaacaacac aaatcaagag aagacgatca
    11341 ctttgaaaga agaactggac ttttgcgaaa aatatgtcta tttatatcaa atgcgttatc
    11401 ctaatcgagt ggcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta
    11461 aatttgtgat tcaaccatta gttgaaaatt attttaaaca tggtattgat tttactcgat
    11521 ttgataatgc cctaagtgtc aaagtcttac aggaagacaa gcgggtaaga atcatcgtca
    11581 aagataatgg aagagggatg actgaaaaac ggctaaaaca ggtggaagaa aaactgtctc
    11641 atcccaaagt ggaacttcac gaatcaatcg gtctgcaaaa cgtgaatgaa cgtttacgtg
    11701 ccaacttcgg cagcagttat tacatgtcgc ttgagaataa tgagacaggt ggactgactg
    11761 tttctattac attcaaggag gggtgaacat gtaccaggtg ttattggtag atgatgaata
    11821 catgattttg aatgggttaa aaaagataat tgattggaaa agtttagggt tccaaatagt
    11881 agcaacagcg gagaatgcca aagaaggact atctgtcctt gagcaacagc ggattgatct
    11941 agtggtaacg gatgtgacga tgccagaaat aaacggactg gaatttattg aagcggctca
    12001 aaaagaacat cataattttg aatttatgat tttgtccggg taccaagaat ttgattattt
    12061 gaaagaaggc atgcaattag gcgcagtgaa ttatttgatg aaacccgtga ataagttcga
    12121 attgattgaa agcctcaaga aaatcaaaac acggttagac cagcaaaatg aacaaaaaaa
    12181 tcagcaagaa atctatcagg aactattatt cagtcaatgg ttgaatgaag agttagatga
    12241 agcaagtgaa gaagaaataa taggacaaat cagtgagaaa cagcatagaa tagtattgat
    12301 ccaattatca agaattcatg atcctcttat caatacatgg ttgaaagaac accagcaaat
    12361 cttttattat caaagaaatt atggagaccg tatattgatt acgctgttgt tggaggcaga
    12421 tgaagttgat agattttgct gttttgtcca agaaaacatc cctgatcagg aatggctgat
    12481 cagtatcggg gaagagacag attcattgga caagatccct gaaacatttc aacaagcgaa
    12541 agacaatcta caattacatc aattttatgg agataaagac gagcatgttt tttacgcaat
    12601 acaagcaaca ataaaagagc aaaccatcga tttttcaagt ttcagacgtg tgttgcaaaa
    12661 taaacgatta gatgatgccc aaaaaatgat cactgatttt ttcgagcaat ttcagttagc
    12721 agccatgatg ccggaagaca tccggtactc cagcttttta ctgtttatgg agattcaacg
    12781 ggaattgatt gatctagaag atgaggaata tttacaagga atcgaaaaaa ttcatcaggc
    12841 aaaaacggtg caagaactcc atcagcttct tctgtcgttc gtccaaaagc accagagaca
    12901 gaaaaaatac agtggcaatg tagaaaaagt aatcgaaatc ctgcatcagc attatcaaga
    12961 accacttaca ctcaaagaag tgtcagaaag tttgcattta aatgtcatgt atttagggca
    13021 gttgttcaaa aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tgcgtatgga
    13081 aaaagcaaag cagctgcttt tgcacagtaa tcaaaacgtc aatgaaatag caagtgagat
    13141 tggctacaac aataccacgt atttctctaa attattcaag aagattgtcg gcagatcccc
    13201 taaggagtat cgggaaaaaa gaggagaatc ctaaaaaaag aagcgtatgg aatgaatcaa
    13261 tgttcaaaag tttgcatctg cggtaataag cccaaccaaa cgaaaatagg aggagctatt
    13321 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct tttgttagtt
    13381 atcgtttgat aaagaaaaag gcaaatcttt tgatcgatac ttcttaggat gagggacgat
    13441 acctaattgg taagaaatct tgccgccttt cagcaaatct tgatgggtaa aatataattt
    13501 cgtatattct ttgtccatcc gattgatttt atggataaat tgctgttgcg gccgattagg
    13561 agtggcttca atcgtcaatt cctttccatt tgacaaaagc acaacagcct tgtccattaa
    13621 aggcataccg ataacatatt caccgattcc tggtgtgaca ggatagaatc ccagactatt
    13681 gaagatatac caagcagcca tacttccgtt gtcctcatct ccagggtaac ccgttgaaga
    13741 atcgttgaaa gtttgagtca tcaattgttt aagcaatggc tgtgccattt cgggttgtcc
    13801 aatatagctg aacaagtatg ggtaatgaaa acttggctga ttggagatag ctaactgccc
    13861 aaattctatc gcagccatct cgctcatttc atgaatctca aaaccatatc ctcctgtttc
    13921 aaatgtcgga gattgattgc acaaatcaat gagtttttct ttgaatctct cactgccgcc
    13981 atatttttcg atcaaccctg caaaatcatg ataaacagca aagctgcttt gccaagcact
    14041 gccttctgca taatcttgtc cccaacgatg agcagaaaat ggttctctga atttgccatt
    14101 tttatctttg gaacgcatcc atcctgtctc atagtcaaaa atattttgat agttcaatga
    14161 tcgttgctga tattttttca ccgtttcgtc ctctgctagt actttggcaa cttgactaat
    14221 acaaaagtcg ctgtaagcat agtctagcgt atgattgacc gattcatggt agtgattagg
    14281 aacatagccg tatgtcaagt aatcctctgt cccttgtcgt ccataatttg gatgattact
    14341 ttgaactgta gcgcctttct tcattgctgt tagaaattcc tgcatcaagt ctggccgaat
    14401 atcttttaca gcagcatctg caatgactgc atcaattaat gtcccaggca ttagtccacg
    14461 ttcgtcagga gacagccatt ttggcaaata gccactttct ctatagctat tcaaaaaacc
    14521 ttccagcatt tcttcgtact tttctacagc aatcaaagaa taaagaggat aaaccgtttt
    14581 atacgtatcc caaaacccat tatttgtata gagaacacca ggtttcacag atcgactagt
    14641 cgtatcatag tggatttttt gcccactagg atccatttca taaaatgtct gcggaaataa
    14701 aaacactcga tagaagttat gataaaacgt ccgcaattgt tgttgatcat ggtgtgtgag
    14761 ctgtatacga tcaaaatagg attgccattg tttctggctg ttagcaaggt aagtctcttt
    14821 actttggttt tgttctagtt ccaggttata agctgcttgt tccgagctga taaaagatgt
    14881 cccgaatcgg atgatttgtt tttttgtctg ttcaaaatac aacgggatcg aatcgctatt
    14941 tttgttggaa acagctaact catcaattgt taaaggatat tcaaatctca atataaaata
    15001 aaaagaaaaa tcaggatctt ctgaaccagc aaaatgtgtg atttttcctt ccactgtgta
    15061 aggatctata acaaagagct gatgacgtcc cggaaaggat aaaagtaaac cgctatcatt
    15121 tctgctgtaa tcaatagata agattccgcc atacatactg ggtatcaatg tagagcggat
    15181 gccataacgc aattgacgga tcgacagatg agtcggacaa aatatacttt cttctgggcg
    15241 gtaagaactt tgagcatgaa aaagggtatt ttcctgtaat ttaccattga ttggcgtgag
    15301 cagcatatga ctaaaatcgc ccatccaagg acttggttgg tgggtaagtc gataaccttg
    15361 aaaaacacga tcctcaggat gaaaccacca gctgccgttt tgatcggtcg tttgcggtgc
    15421 aaaataattc atcccaaaag gaacgccagt gtaaggctgg cagttcccgt tagagaaatt
    15481 tggctgatta gctgtgccat gtctagtatc tatttgaccg atattcattt tatttctcct
    15541 ttgttgattg tcttttgata aatgaaactg gaaatttgct gtcggaagga cgttcgtttg
    15601 tctctaacca agtgatcaaa gcctctccag caagcttgcc taactgttca aaatcttgtg
    15661 caatggttgt cagcggtgga tcaacaagag cggctgcttg gatatcatca aatccgatga
    15721 ttgaaaaatc ttcaggaaca tgataatcat gctgtctcaa gaaattcatc gtctggatag
    15781 caacaacatc gttttcgcaa acaaaagcgg tcacttcata cttagtgata taagaaagca
    15841 attcatcgaa tgtcgcgctt ttgtcatcca gcgaagtgtg gaaagccaaa ccagctttgt
    15901 ctaatgcttc aaggtaacca aggtaacgct gacgtacgga ttgtggatga gtctgtgcac
    15961 caaataaata agcgattttt gaatgtccct gttcgatcaa atgagaagta gcgagcacac
    16021 caccttggaa gttgtcggaa agcacggtag gaaaaggcaa ttcatagatt tttttatcca
    16081 atatgataac agggaattcc tttaatgaaa gctcaaaaag caagtcaaga tgctgttctg
    16141 tatcaaaggc ataataaatc aatccatcaa attcatggat gagttctttt gccgttttat
    16201 tttgtaaaaa atcatgtgtc gtcatcatga catccatctg tttatcctgc atcacaggat
    16261 tcaatccctt ggtaaaatca ccaacagaca aatcagcgac atatggaaga agaaacagaa
    16321 tccgccgtgt ttttgtaggt gaaggaagca tctgctccgg agaagctgat ttaacaaaac
    16381 tgcctttccc acgaacgcga taaatcagcc ctaattgctc taattcagtc aatgcacgtt
    16441 ttgaagtgat tctgcttact ttataagtat gggaaagttc cttttcagtt ggtagttggc
    16501 tatctacagg taattcacca gttaaaatag ccatttgcaa atcattaaat attgtctggt
    16561 acaatggttg attcatataa acacctctta atgatatatc atatttaaat aataccataa
    16621 tagatgaaaa agtaaataaa aatttatttt tttgaaagcg tagacataaa atgacacaag
    16681 aggtataatc tttaaagata tatcaaatga aaagaggaaa tttatatggt ttataaagat
    16741 ataccaaagt cagtacaaaa atttatgaat gatatcacac aaaaatgcgg gaaaaaccat
    16801 gcggactggg caacgaattt tacagcagca tttgccaaca cattattgac aacagtcaaa
    16861 aagcaaaatg acggtactac ttttttattg acaggagaca ttcctgcaat gtggttgcga
    16921 gattctactg cacaagttcg tccttattta gtgattgcaa aagaagatga ggaactagct
    16981 gacatgatta gtgggttggt caaaaagcaa tttttttata tcaacatcga tccatatgcg
    17041 aatgccttca atgaagaagc gaacggtgcg ggacaccaaa cagatcacac aaaaatgaac
    17101 gattggatct gggaaagaaa atatgaaatt gattctttat gctacccgat acagttggcc
    17161 tatcttcttt ataaaaatac aggaaaaacg gaccaattca acccaacttt tattgaaggg
    17221 atcaaaaaaa tcttaggcgt gttcgaaaca gagcaggatc atgaacactc tccttataca
    17281 tttatacgag atacgacaag aaaagaagat acgctgatca atgacggacg aggagccaaa
    17341 acaactttta caggtatgac atggtctggg ttccgtccta gcgatgatgc ttgccgttac
    17401 ggatatctgg taccatccaa tatgtttgcg gtggtcgtgt taggttatgt ggaagaaatt
    17461 ttccaaacga tactcgatga taaagaaatt gtacagaaag cacaaatatt gaagaaagag
    17521 atccaagaag gtatccaaaa tcacggacgg acggttaatc aaaaagggca aacgatttat
    17581 gcatacgaag tagatggaat ggggcacgcg acggtcatgg atgacagcaa tgtgcccaat
    17641 ttgatcgcgg caccttattt gggttactgt tcggaaaaag aggaagatta tcttcaaaca
    17701 agaagaacat tgttgagtaa agagaatcca tatttttatg aaggaaagta cgcaaaagga
    17761 attggttctt cccatacgcc aaaagagtat gtttggccaa ttgcactagc tatggaagga
    17821 atgacaacac cagataaatc tgaaaaagaa cggctattga atttgcttgt agcaactgat
    17881 gcaggaacac atcttatgca cgaaggattt gatgtgaaca atcctgaaaa ttatacaaga
    17941 gaatggtttt cttgggcaaa catgatgttt tgcgaattag tcatggatta ctttgatatt
    18001 cgagtagaaa aataggaggt actgtaaatg aaaaagaaag tatatatcat ttctcatagt
    18061 cactgggatc gtgaatggta catggcttac gaacagcatc acatgcggtt gattgaattg
    18121 atagacgatt tattagagct atttgaagtt gatccgtcct tcaacagttt tcatttggat
    18181 gggcaaacga ttattttaga tgactatctt caagtaagac cagaaaaaag acaggcgctc
    18241 caacaagcaa tcaatgaagg aaaattgaga atcggaccat tttatatctt gcaagatgat
    18301 tttctgatca gttcggaatc aaacgtgaga aatatgatga ttggtatgga agaaagccgt
    18361 aaatggggaa cccccgtcat gttaggttat tttcctgata catttggaaa tatgggacag
    18421 acgccacaat taatgaaaca agctggtatt tcagcagcgg cgtttggccg tggggtaaaa
    18481 ccaattggct ttgacaatca agttctggaa gcagaaaact attcttctca atactcagag
    18541 atgtggtgga aaggacctga tcaaacagaa atttttggtc tgttatttgc caattggtat
    18601 agcaatggga acgagattcc cgtagaaaaa gaagctgctc gtgtcttttg gaagcagaaa
    18661 ttagcagatg cagaacaata tgcatctact gatcatctat taatgatgaa tggtgttgat
    18721 catcaaccag tacaaaaaga tatatcaaaa gcaattcatc tcgcaaatga gctgtttcca
    18781 gattacgagt tcatccattc taattttact gactatctgg aagcagttcg acaagagttg
    18841 ccagaagact taggttcagt agaaggagaa ttgacaagtc aagaaacgga tggctggtat
    18901 actttagcca atacagcatc tgctcgcgtt tatttgaaac aatggaatac caaggtccaa
    18961 cggcagctag aaaacatcac agaaccactt gcgacgatgg cttacgaagt tacagggatt
    19021 tatccccacg atcaattaga ttatgcttgg aagacattaa tgcaaaatca tcctcatgac
    19081 agtatttgcg gttgttcagt cgattctgtc catcgggaaa tgattcctcg ttttgaaaaa
    19141 gcagatgaag tcggaaagta tcttgcacaa gattctttag aacaactggc cacagcaatc
    19201 gatacaactg gatttccaaa agacagcttt ccatttgtcg ttgtaaatac tgcaggaatg
    19261 gataaaactg gcgaagcaga aataaccatc gaattagaaa gaaaaagatt cgctgaggga
    19321 ataccggaac atctgtatca agagttagaa cagttgccta gacggaagta tcacgtagaa
    19381 acaaaaagcg gggccactat accagctatt ttatctgaag aaactgtaca atttggttat
    19441 gatttgccga aagatcgttt ccgtgtgcca tatatggcaa ggacaatcaa ggtaacttta
    19501 ccaataaaaa acatgccagc gttttcttgg gaaacattcg cgcttgtaga aggggaagcg
    19561 aaagcgaaag cagaagaaaa ggaaacaatg atccgtcaga gtggacgaat cgtcgaaaac
    19621 gcccacctgc gtttgacgat tgacaaaaac ggtgccatta caatggaaga tcgtaaaaac
    19681 aaacgaaaat ggaataacct gcatattttt gaagatatcg gtgatatagg aaacgagtat
    19741 atctttaagc aacctgtcta tgatcaacca atcctatcca gtaatcaaga ggactctgaa
    19801 gtaaaagtat tagtagatac accagaaata gcaaaaataa gtattctaca ggaaatggag
    19861 atcccagttt ctgcagacga gcgtttagag aaagaacaac ggatggttgt agaatttcgt
    19921 tatcgaaaag cagaacgatc taaagaaaaa cggatccttc aaatagaaac gatcatgaca
    19981 gtcagaaaag atagtaaaaa aatcgacttt gaaacgactc ttgacaatca aatgaaagat
    20041 catcgattgc gtatcttgtt tcctacaaag ttacatgtgg aacatcacga agcagatagt
    20101 atttttgaag tagtaaagcg cccaaaccat gtatctagga gctgggaaaa tcccacgaat
    20161 ccgcaacatc agcaagcatt tgtcaatatt catgatgaac agaacggtgt aacagttggt
    20221 aactttggtt tgaatgaata cgaagtaaca gcagatggac aaatcgcggt gacgctactt
    20281 cgctgtgtgg gagaactagg agattggggg tattttccaa caccagaagc acagtgttta
    20341 ggtaagcatc agttcaatta cagcgttgaa ctacatggac cagaagaaac attctctact
    20401 tatttgcatg catacgcggc acagattcct ttttccactc aacaaatcaa acatcatgaa
    20461 ggaacattgg catcaaaaca acaatattta acgataaaaa gtgaaacgtt tgcaattaca
    20521 gctttgaaac ggagtaagtt tagtgataaa gtagtagtga gaggtttcaa tatgtctagt
    20581 catttagaaa aattagaaat aactaaagac aatcgaaaaa cggtcatatt gaatctgctt
    20641 gaagaaccaa cgcagcaatc ggttgtcccg ataatccaac cttatgaaat tcggacaatc
    20701 ggattcgagg aggaaaacta aatgtatggc ggaatagaag caggcggaac aaaatttgtg
    20761 tgtgcggttt cagaagatgg agagattttg gaaaaaacaa gcattcctac cacaacacca
    20821 gaagaaacat tggatcaagt atttgaattt tttgatcgtt atgaactgaa tggaatcggg
    20881 attggctcat tcggcccgat tggtatcgac caaacaaaag ataattacgg atacgttttg
    20941 gcaactccca aaaaaggatg ggcacaattt gattttctcg gcacaatcag gaaacgctac
    21001 aatgttcctg ttgcttggac gacggatgtc aatgcggctg catacgggga gctgaagcaa
    21061 ggggcggcac aaggaaaaaa tagttgtatt taccttaccg tgggaacagg aatcggtgca
    21121 ggcgttgtga tcaatgagga aattttttca ggaatcgcac atcctgaaat gggacatatt
    21181 tgggtaaaac gccatccaga tgatcaatat gaaggcactt gtccttatca taaagactgt
    21241 ttagaaggat tagcagcagg tccgtctatt gaggcacgta taggaatcaa agggcaagat
    21301 ctgccacaag atcatccgat ttgggatatt caagctttct atattgctca agcactgatc
    21361 aactatacgt tgacgttagc acctgaaaag attattttag gcggcggggt aatgaatcag
    21421 gatcatctcc ttcaaaaaat tcgtcagcaa tttgttgaat tgatgggtgg ctacatggaa
    21481 acacctcaag taagtgaata tatcgttcgt tggggactgc cgaatgaaag tgggattatc
    21541 ggtagtttgc tgttagcaga acacgaacat caaaagtaag cagacaagtt acacaaaaag
    21601 aacgtagtct tatcagcaga taatgatagg actacgttct tctttctatt tccacaatcc
    21661 gggtaaagct ttaccaacta ttttttcttg agtaaatgga tgaatcatgt gcagttctaa
    21721 tgcatggagc atcagtcgtt cagctggtct attttggtaa agagggtctc cgacgattgg
    21781 atgccctata tgagctaagt gaactctgat ctggtgcgta cgaccggttt ccaaaacaca
    21841 atacacttgt gtttttccag aaagtacttt gtcgacagtg acatgtgtca aggcgttctg
    21901 accattttta tcatctacga cccgttttct ccgatcatgg cgatcgcgtc cgattttatt
    21961 ggtaatagtc ctatctttgg cgattttccc ccatacgatg gcttgatatc tccgatagat
    22021 cattttttgt tccaacattc ttccaagtat cggcaagaca aatgggtttt ttgcaaataa
    22081 aacagcgccg cttgtttctt tgtccaaacg gtggacaaca taaggtcgtt gttttttcgt
    22141 atcgagataa gcagctaagt gattgagcaa ggtactgttt tctgttggtt cgtttggatg
    22201 agtcttcatt cctgcaggct tatccactat aatcaaatgt tcatcttcat ataaaacacg
    22261 aatgtgcttc gcatctccta attggacttc ttgataatta taatctgttt cttcgaataa
    22321 caatgtgatc tggtcattgc cttttacttc atgatggaac aaagcaggtt gttgattgat
    22381 ccaaacgttt ttacgtatac gcaaaaagtg ccgaaccttt ctaggaacta accattcctt
    22441 ttcaagcaag tcgcgaatcg ttgtttcagg gtggttcata ggtaaagtaa tcgtgatttt
    22501 cacaagtgta ctcctttcgg taaaattgca gactaattgt accattttta aaacatattt
    22561 aagaaaaaac ttacattttt ctgttcattc ttatgttact ctacttgcac tagcgaaaaa
    22621 aaacaaatcg aactacaact taagcaggaa gaaaacactc tttcattatg gtaaaataag
    22681 acgattgaat tgaatagaca ggagaagaag cgaatggatt ttcaaagcat tttaggaaag
    22741 ataaaagctg ctttgatcag tttttggcga tggatcagac catatctcgg acaattccat
    22801 cgatggcgaa agcgcatttg gaaaaaatat catgtgaaca aattgatttt gctgattggt
    22861 ttgatctgtg tcttagtgac aagtatctac ctttttattt tagccaaaca agcaaatgtc
    22921 gaaacgttaa aatctggatt aagccaatcg acagtcattt atgacaagaa aaatgaagaa
    22981 gctggcacac tgtacgccca aaaagggaca tacgtagaat taaacgcgat ttctccctat
    23041 atccaagatg cggtgatctc gacagaagat aggaattttt acgaacatca cggatttgat
    23101 atcaaaggaa tcgcccgtgc ggctgttcgg atggttttgt ctggcggaac atcagggggc
    23161 ggaggaagta cgatcactca gcagcttgcc aaaaatgctt atctgacatt agatcaaaca
    23221 tttgatcgaa aagcgaagga attatttttg gcaatcgaga tcgaaaagaa gtacagcaaa
    23281 gaagaaatct tgaccatgta tcttaacaat gcctattttg gcaacggggt atggggtgtc
    23341 caagacgctt ccagaaagta ttttggagta gatgcaacga atgttacgct atctgaggct
    23401 gctactttgg caggaatgct gaaaggtcct ggtatttata atccgattga tcacccagca
    23461 aatgcgaatg atcggaaaaa cacagtatta agcgtaatgg aagaaaacgg aaaaatcaca
    23521 aaagaacaag aacaagcaga aagccagaca gacatcagtg cttatcttca tgacacatac
    23581 gacaattcag attctggtta tcgttatcct tattattttg atgcagtgat cgatgaagca
    23641 gtgaacgagt atggtcttga tgaagaagat gtcatgaata aaggctataa aatctataca
    23701 gctttagatc aggattacca aaagcaatta gaagctactt atcaaaataa tgcattattc
    23761 ccagcaaatg cttcagatgg tgccatggtc caatctgctt cagtagcttt agatccgaat
    23821 acaggcggtg tacaagcatt agtaggacga agaggagatc atgtctttag aggattcagc
    23881 tttgctacac agatgcgccg ttctccagga tcaacgatca aaccattaag cgtgtataca
    23941 ccagcgttgg aagctggcta caagccttct agtgtcttga aagatgaacc gcaaagttat
    24001 tacgatgcgc ataattttga cgggacatac caaggagaag taccgatgta tcaggctgtt
    24061 gcacaaagtt tgaacttgcc tgctgtatgg cttttgcatg agatcggttt acaaaaagga
    24121 tatgataaag cagaagaatt cggcctgcct ttagcaaaat ctgataagta ttatggctta
    24181 gctcttggcg gattagaaaa aggagtatct ccaatgatca tggcgggtgc ttacagtgca
    24241 tttgccaatg aaggtcagat gtatacgccg catctcatca cgaaaatcgt tgattcaact
    24301 ggagcggtga tcatcgataa tacaaacaaa aagccaaaac aggtcatatc aaaggaaaca
    24361 gccgatgaaa tgactagcat gcttcttggt acattctcaa atgggacagc agctgctgcg
    24421 aatcctgcag gttatacgat agcagggaaa acaggaacca cagaaactaa tttcgatgcc
    24481 acgaaagcaa atgaccaatg gatgattggg tatacgccag acgtagtgat ttcgacatgg
    24541 atggggtatg aagaatccag caagctgcat tatttggaag ggacaagtgg aaatgttgtc
    24601 gggaaagtct tcaaatcagc ggcagaaggt atccttcctt atacttccaa aacaggattt
    24661 agtgtagcgg atgcctatgc cacaggaggg caagtagtag cagcagatca agttggaaat
    24721 acaggtaatt ctaatggaga gtctggaaat tggcaagata acttgaatca atatggtgaa
    24781 caagcaaaag aaggattgaa gaatttcggc gatatggtaa aagaaggcgt tcagggaatt
    24841 ggtgaagcgg ctaaagacct ctggcggaaa tatcaaggag agtagaccct ttgaaaacga
    24901 ataaccccat gttacaatag agagtaacta ctaaaagaaa agagggcata acatgtcaaa
    24961 catctacgat agcgcaaatc agatcgaacg tgagatccgt gaattaccag aattcaaagc
    25021 gttgaaagaa gcgtacgaga aagtacaagc aaacgaagaa gcacacaagc tattcaaaga
    25081 cttccaagcg atgcaaatcg aattacaaga aaaacaaatg agcggacaag aattcagcga
    25141 cgaagatgca atgaaagcac aagaaatggc tgtaaaaatt caatcagaag aagtaatcaa
    25201 tgatttaatg caaaaagaac aagggttcag tacgatcatt aatgacttga accggattat
    25261 catgacgcca gttcgcgacc tttacagcga atagaactat tttatgaaac aacagaggtt
    25321 gtgaaagagc tgttcagcct tgagcgatga caaaattgct tcttattgcc gatagactgt
    25381 cgcttgaaca ccatttacca agaggtcgtg agcctaccgt ttaactctga gttacaagga
    25441 acaatttcaa aaaaagcttc tcatgtttga tgactattgt gatttgtgac cgaggagttg
    25501 gcaggagaac accgtttatt cagaaaaagg agagacgaca aaactcttgt cgtctctcct
    25561 ttttttgtca ttcatttttc aaacggtaaa aatagccctc ttgagctaac agctcttcgt
    25621 aagtacccat ctcaacgatt tttccttcat cgatcacgac gattttatct gcctctttga
    25681 ttgttgaaag ccgatgagcc acaataaaag tagtgggtgt ttctgtcagg taattcaggg
    25741 cctgctggat ttttttctct gactggttgt ctaaagctga agttgcttca tctagcaaaa
    25801 tgattttcgg ctgacgtatc aatgctcgtg caatagagat acgttgtttt tggccaccag
    25861 acagcttgtt gccatgttcg cctactaagg tatccagacc atcaggaagc tggtcaatca
    25921 gtgaagaaag ttgcgcagct tcgatgactt catttaaacg ttcttttgat acgtttttca
    25981 agccataagt gatgttctct ttgatcgaag cggagaacaa caaagtggtt tgaggaacaa
    26041 cggctaaata attacgcaca cttcgcatat ccaattcatc caaaggctga tcatccaaga
    26101 aaatcctccc tgaagacggc tggataaatc caatcaataa gttaaggagt gtagattttc
    26161 cagaaccaga tggcccaaca aaagcaattg tttctccagc tttcacctct agagaaaaat
    26221 gatcgataat cggcttgagt gagtccgggt aatgaaaaga aacattctca aaacgaaaat
    26281 ttccatccaa atgttggatt tttttcttac cgtgataagt ttctacatcg ccagcaatca
    26341 acagttcacc aatagaagta atagattcca tgccttttga aatcgtagga atcagtgtga
    26401 tcattccaga aacggaattg acgatcgttg tgaaatatgt ctgatacatc acgacatcac
    26461 ctggttggat acgcccttgt aaagctaata tcccagtgaa aatcaaacaa atcagctgaa
    26521 atatctgaaa actagcccag ctgactgatc cgaaaacaga ttgcaggata tctaagtgga
    26581 atcctttttc atagatttgg gtaaaatgct tatttaagcg agtggtttcg tgtttttcta
    26641 acccatgagc tcgagcaatc ggtgctaatt cgatcacttc gatcactttt gcagaagttt
    26701 cttccatttc ttgccggaaa tctgaattgg actgtctgat ccttttccgg aaaaaaagag
    26761 taactaacgc ggcgagaggg acagtcaata agaaaaacaa gaaaacgaca ggacttctat
    26821 aaagcgtgat acctaaagcc acgaataaat tgacagcaat gttcaatccg ctgacaaata
    26881 cttgagagga caatgtttgg atggcttcta catcacggat gatctttgat tggatccggc
    26941 cagattgcaa ttctttttga tagggaatag aaagttcttg gattttcttg ataagggtcg
    27001 ctcttagtcc agcttctact tgccggatcg aagtgctatg gtattttaca tgaaaataat
    27061 tcgtcaatat attttgaccg atcagacaag tcatcaaaaa agcgttgaaa taaatcgcct
    27121 gcaattgaga ggaatctttt gatgaagctg cattgattac attggcaata gcgatcggca
    27181 ttaaccaaga tggaagatgt ttgataataa aaaagagtcc tgacatagca aagcgccaat
    27241 aattcccctg ataaagatgc agcaatgtct gaaaagcatt tgcatgatat ttttgataac
    27301 ttttctgtat tcgttcctcc ataatcttct ccttaacaat cttggtaaat aaactgcaag
    27361 tgttctattg cttcaggaac ataaatctca ggagtagctt caagagtggc gtacattaga
    27421 ggacgctcat atttgatata atgcagtacc ggtcggaaat ctaaacagcc tttgccaaca
    27481 ggaaccatgc ggatctttcc attttccatg atgtagtctt tgagatgaag cgctgtaata
    27541 caaccctgta aattgtccaa ggcaccttgg atcactttct cctgatcagt atgattttct
    27601 ttatgcatca aattcgcaca atctaaaata atttttaaat ttggtgaagc tacttcatcg
    27661 actaaacgtt tggctaactg atagttatag attggatgat tgatccctgc ttcaacagca
    27721 acagttacgc caaaattttc tgcatgagcg actaattcaa tgacagaatt tctagcgatc
    27781 tgataagctt cttcagtaaa attttctttt gtgtaaccga cagagacact accagtttct
    27841 gtagcgatca tcttggcttg ataggcagag cacagagcaa tttgctgttt gaaagttgct
    27901 aatgcttgtg cacgaacttc ttgatttttg ctggataggt tgatatagca tcctaaaatg
    27961 gaaagttcga tattgttttt ttggaagtat ttaccaaaat aggcagcact tccaagtgtc
    28021 atggcattta atttaagttg ttcaggaaat gctttttgcg gtgcaaattg gatatgggac
    28081 aagtgaagct gtgatacttt ttgaacgatt tcttcacgat ttcgaacctc tagatcatgg
    28141 gcacgaatgc ctaaatttaa tggaaacatt tcaaactcct ttttgtaaat aatcagcaag
    28201 aggcagacgg gattctttta tcaattcagc aattattcgg gcaactttat cagcgccttc
    28261 tggactaaag tgggtattat cctttagtcc ttctgggtaa ttttcacaac tattaggcat
    28321 aagatgcaga tagaattttt ttgtctcttc ttttgaaaag gtctggaaat attcttgtga
    28381 tcgtgagaaa acatctaaaa gcggaatatg gtatttctca gcaaatgcac gcatcgcttt
    28441 tggatagtct ccaagtatat ctggattcaa tgttccattt tctaagtaat ctcgtcgcgt
    28501 cacagatgtc agcaagacca tgtggacatt cgcttttttt gcgcattcaa aaaatatctg
    28561 cagatttttt aaatagctac cgaagggaag cgtacctctt tcttcttgga ttttctggtc
    28621 gttatgtcca aattgtacaa ataagaaatc atttggctga aactgtgaag ctaaacgggc
    28681 taaccttcct tcctggataa atgttttagt gctgcgtcca ttctttgctt ggttattaat
    28741 ctggatctca ggtgaaaaat aagcagatat tttttctccc caacctgtct cgggacgctt
    28801 gttttcatct ttttctgccg cagtagaatc accggcaata tggatagtag gcatgttatc
    28861 tcttctttca taaaggttta taggcaatga agcagaacag caagagaaga agacggagca
    28921 aatttggtcc gtcttcttct ctaaaacgtg gataaacgga ttccgttatt tcgctatcct
    28981 gtttttgatt cgttactgtt actattaatc aattaggcaa aaaagccaaa gtaattttta
    29041 gcatttgtat aactaatcgc ttgaacgaga cttcctaaaa gttgttcatc ctcaggtgct
    29101 tcccctcgca tgaccatttc gcctaaaaat tcacaaagaa cacgacggaa atattcatgt
    29161 ctagtgtagg aaaggaaact tcttgaatct gtcagcatgc cgacaaaatt ccccaaagca
    29221 ctaccatcag caaactgtgt caattgatcg cgcataccag ctcgagtatc gttgtaccac
    29281 catccagaac ctaactgcag ttttccattc tgttcttttt ggaaacagcc catcaaagct
    29341 aaaatcgcag ggtagtcatt tggattcagt gaatagagga tcgtctttgg caactgattc
    29401 gtttcttctg cacgattcaa taatttttgc agaggaatag tcaaagaaag atcattcatt
    29461 ccatcataac cggtatcggg tcctaatttt tccaacattt gtgtattaca attacgataa
    29521 gcatgaatat gcagctgcat tgtccaatta tgagaatgat agaagtgggt caaacgtatc
    29581 agtgtttctg tacgataagc atcgatttca tctgaagata atagtttgtt agctcgtgct
    29641 ttttgaaaaa tctcttctaa tactgtttca tccgcttctt tatagcgtaa ggtgtctaaa
    29701 gcgtggtcgg aaacacgaca tccatgttga tggaagtatt ctatccgctg ccctaaaact
    29761 tcgatcagtt cttgataggt ggtaatcgtt tggtcagctg cttcttccag tagtgtaatc
    29821 catacattaa atccttcttt ttcaatattg agtgctttgt ctggacggaa tgaagggaga
    29881 actttcaaat ctggttcttc atgagcaagc aattgatgat agtgtaagtc atctgctgga
    29941 tcatctgtcg tacaaatcac ttctacattc gattttttcg cgatttcccg ccgacggaaa
    30001 tcaggagatg ccaaaatttc atttgcttgt gtccaaattt tctctgcgtt ttcttcattt
    30061 atcaacaagt caatatcgaa gaaacgcttc aattcaagat gtgaccaact gtaaagcgga
    30121 ttgccgataa tttttggtac ggtttgacac caagctaaaa atttttcaaa atcagaagca
    30181 tttcctgtga ttttttcttc aggcacgccg caagctcgca tcaagcgcca tttgtaatga
    30241 tcgcctccta accaagcttc tgtcagattg ctgaaattgt tgttttcgta aatttctttt
    30301 ggatctaagt gacagtgata atcaataatg ggcatatttt ttgcgtagga atgataaaga
    30361 tttttcgccc aatcgtcttt taataaaaaa tcatcagata aaaacataaa tcttccttct
    30421 ttctttttat actgttttat gaaaatcaaa gtgttactcc gtttttaaaa atggcaattt
    30481 ctctaacgtc attttgttca tttttcgttt cttctccact agccacagcg atgatttttt
    30541 gaacaaatga ttctaagatc acttccattg gttgttctaa taagacgcct gcattaaagt
    30601 ccatccaatg acctttgcgt tcaaaaagag tcgtgttcgt agataccttg accgtaggga
    30661 catatgagcc aaacggagtc cctcttcctg tggtaaacaa gaccaactgg caatcggatg
    30721 aagcaagagc agaggcagct acaagatcat tcccgggagc ttgcaataaa ctaagtcctg
    30781 gttttctgat tttttcgcca tattgcagta catcaacgac aggagagctt cctgattttt
    30841 gcgtgcagcc taaagactta tcttctaatg tagtgatccc acctgcttta ttaccaggag
    30901 atgggttttc gtaaactggt tccccgtaag acaagaaata ctgtttgaag tcattgatca
    30961 agtcaacgat tgaaccaaaa actttttcat tttccgctcg tgccatcaat acttgttctg
    31021 caccaaacat ttctggcact tctgttaaga tcgtgctacc tccttgagca atcaagtaat
    31081 cagaaaatgc accaagcaaa ggattggcag ttattcccga tagaccatca gaaccgccgc
    31141 atttcaaacc gacattcaac gtagagagcg ggactggtat gcgctggtcg ttttcgaccg
    31201 cggttaaaag ttcttctaat agagctacgc cttgttcgat ctcatcatag acatcttgag
    31261 ccactagaaa tttcactcgt tcttcatcat attcacctaa aatttttttg aattcaggaa
    31321 cggtattgtt ctcgcaacct aatccaaaca ctaaaactcc gccagcattt ggatggttta
    31381 ccgcgtctgc taagatttct cgagtatttt gatgatcttt tccaagctgt gaacagccgt
    31441 agggatgctt taaaatcgta atatgatcaa actgtcccag atccggatgt ttttctttga
    31501 actgtttgac gatcagttca gcaattccat tcacacaccc aacggtaggg actaagaaca
    31561 agtcattacg aatccctact tttccatttt ttcgtaagta gccttgaaat gttcggttct
    31621 caaaaggata atgacttgga tggacatctt tttcatatcg ataatccaat tcaccagaaa
    31681 ggttggtttt tacattatga gtgtgcagcc attgaccggc agttacgtct gtcgtcacgt
    31741 gcccgattgg atagccatat ttgatcacgt tgtctccagc tttcaaatca ctcaatgcaa
    31801 tcttgtgacc ttgcgggata tcttcaagag tggtgatcgt ttgctgatca atcgtcagtt
    31861 gtgtttgctt tgggataggt gttaatgcga caacaacact gtctcgttga tttaatttca
    31921 ttgttggttg cagggtttca agcatggacc atcgttcctt tcatttcttg taatactgct
    31981 cggacacctt tttgatcgat ttttgtagcc atttcttgaa cagtatctgc cacattcggt
    32041 aattgagaca aatcttctcc ccaaagcgca gcgttttgca ataatcgaag aatagctgtt
    32101 tcattatcag accaagcatc aacaaattcg cttatcgtct tttcatcatc atggggcttt
    32161 acttgatcgc ctcgatacat aagaagtaac gcagctagtg aaacagagat tagcggtggt
    32221 acttgatttt tttcttcgat atacttcttg aagaccggaa gcagacgggt gctgaatttt
    32281 gaaacactat ttaatgaaat cgcttctaat ttgtgatggg caaaaggatt caagaaacgt
    32341 tctttgattt gatcagcata aatggctaat tcatctttag gtaaagaaag cataggaatc
    32401 aattctagat cgaatagctg atcaacataa gcggagaata aaggatcttt cattacttct
    32461 tctacagttt ccaattccgc caacaaagca agagggacca tcgctgtgtg cggtccatta
    32521 agcaaatgta ccttacgttc acgataagga gtcatatcat ctgtgatgat gacattcaag
    32581 ccagcctgtt tgaatcgtag ttgattttcc aactctttag gtccttcgat cacccaaagc
    32641 atgaatggtt ccgctttgac catcaattga tcttcatagt tatgtcgttg attgaaaaca
    32701 tctgcatcct cacgaggata acctggtacg atgcgatcaa ctaaactaca acaaaaaaca
    32761 ttttcttttt ctaaccactc aataaaagca ggttctaaat cccatttttc tgcatagcgt
    32821 aaaataattt cttttagacg ttcaccgttt ctgtcaatca attcacaagg aataatggta
    32881 aaaccagctt tgtttaattc aaaacgtcgg tataaaaagg ctgtcagttt gcccgggaag
    32941 ctctgtggtg gcgtatctgt tcgttcatca gtctccacaa attggattcc tgcctctgtt
    33001 gtattggaga cgatataggc taagtcgtca ttttctgcta gagaaagata agcatcccat
    33061 tcatgataag gattgatcat ctgttctact atggtgataa tctcggaagt atcaacgatt
    33121 tcgccattgc tgagtccttg taaaatgacc gtatataaat tgtcttgctg gttcatcata
    33181 ttcccaagac cttgttctaa aggctgaatg atggcgacat ttcctttaaa caattgttgg
    33241 ttattcagtt gctgaatctg ccaatcaaca aatccgcgca taaaattgcc ttcgccgaat
    33301 tgaagaactt ttacgggagc ctcgtttttt ttcactaatt cttttgtcag tttattcata
    33361 gattacttcc tttctgtgtt atgcacacgt taacattttt atataaaatt acttgcagtt
    33421 tacaagtaat tgatcgactc acgaagcatc agtaaggtgg agagctccat tttagattgt
    33481 tcgatcggcc tgtcttcaat caaatccaat aaaagctttg ttcctacttg acacatttct
    33541 ttcaacggtt tgtgtactgt agtaagcggt ggattaagat aggagacgac tggagaatca
    33601 tcgaacccaa tcagagaaat gttctgggga acacgaagac cagctgaatg tacagctctc
    33661 aatgcaccaa tcgctatgtc gtcgttgcca caaaaaacca gagtaggcag gtcagaaagc
    33721 gataataggt actccatgtt ttggaggccg ctttctaagc tgtagtcccc tgttaaaaaa
    33781 taggctggat tggcagggat gcttgctttt tttagactgt ccaatacacc tttttcccgt
    33841 tcttttgtcg aacgaaagtt ttttttccca ccaatataag caatcttcgt atgtccgcac
    33901 ccaataccgt aatctactgc ttgcataaca ccggccgcat catcggcagt aacattgata
    33961 accgctggat cattgatgtg acgattgaca actacgaagg gaaactcttt ttcttttaga
    34021 taatcgataa aaggctgatc ttcatcactt tgactcataa cgattagacc atcataacgt
    34081 tgcaaattga tttctgttaa atcaacagca tcgatacttt cgacagataa gctgtagttt
    34141 gtgtccaaca ctgaatggat acctgcaatg gaatctgcta ggaaatttga tgaagttcct
    34201 ttatgcaaac ttgagaaaaa cagtccgata agatattttt tttgatttac taaacctttt
    34261 gcactatagt tcggcaaata attcatttcc ttagctagtt gccgtatttt ttgttttgtt
    34321 tcatttttga ttaaactgct gttattcaaa gcacgagaaa ctgtcgtatg agaaacacca
    34381 gctgctttag cgatatcttt gatcgttacc attccatcat tttccttact ttttcacttt
    34441 ttgttaacgc attcatcata ttacgataac tacgataagt caatagatct ttggtatgaa
    34501 aagattgcac acgttaaaaa ttacttggct ttttattttt aattatgcta ttcttaaaaa
    34561 cggggaatgc tcatttaatt agatgagtat caattttaca gaaaaatgaa agccttttac
    34621 ttaaagggaa gagaggaaga aaatgaagaa aagtacagtg ttttataaaa tatttttacc
    34681 gatgttttta ttaggagccg tacttgtcat aggatttagt ttatttattt atcagaatac
    34741 ctatgagtcg atcgaatcga gttatttaac agataaaaag aacctactca ggcaagtcaa
    34801 aacgaacgtt gaatggaaaa tcagaacaat cgaatattcg ttttctacgt atggttcgac
    34861 caaaaatttc tcggatatat tcaagcatcc gctgacatat acaaattact ccacttattc
    34921 tgaaatcagg aaagagctaa actttataga aacaattgtt atggatgata ataattatga
    34981 tttactcagt ttagctggta aatggggagt aatcaatgga tcgttgactc aactgactga
    35041 agaggaagtt gaaaggtatc aacaaaagta cataaataac aaagataatt tattttggca
    35101 aaagaaaaat aaaggaatcg aaatggtgat tcctttacca atgtttgaaa aagaaaaata
    35161 tgcattggga attgctacga tagagaaaca tacaatagaa caagttgttg gggaaagtgg
    35221 agaagatata ctttctatcg aaaacgaaga tggaaagctt ttttctacac agaaagtaaa
    35281 agaagaagag cagccagaag cattagcgga tgtggatcta gagtacaacc agttgaaagt
    35341 gataagagaa aatgggacat cctatatcgt actgaaatca gattataata attggattta
    35401 cagattagaa gttgattcta gtgcaattgg agcaacgatt cgcaatctga gaatcgggtt
    35461 agttgcagtg tctttgacgc tggtaggact gatcggtctc ctttcctatt tattttcgga
    35521 cagatttgcc cgtccaattt ctcaaattca ggaacgtttg aatcttaaat caaaaggatt
    35581 cagtgggaaa gaactagatt tggtcgctca atcagtcagt cggatcatag gagaaaaaga
    35641 agcgttaagt gcacacttag ttacccaaaa gccacaacta gaaacgttat ttgtacttag
    35701 tttatttaga aatcgagtag aaaggcgaga actagaccag cggttgcagc aatttggtta
    35761 tagttctcaa aatgactgct attatacggc cttggttcaa atcgatctgt tagaagatag
    35821 tcacggtggg gagcgtgatt tgctactttt agcaatcaac aacatgatca cggaaattgt
    35881 gccaaaggaa aagcgaatgc tgccgatcgt tttgaatgaa gaaatgcaag ccactattta
    35941 tcgaatggat aaagacgatg cagaagcaag caaaaaagtc atggagtatt gccgactcat
    36001 tcaaaaacgt gtaaaagaat acttgaaatt aactgtgagt ataggtatca gtaaccgatt
    36061 cgaaacgctg aatgaaagca agcaatcggt tgaccgagca aaagaagccc tgtattatcg
    36121 tgtaaatact ggtccgagtt cgatcatatt ttatcaggaa attgttcctg ctgcgcatga
    36181 aaaaacgctt attagatatc cagtagaaga acagaatcga ttgtttgaag ctattcgttc
    36241 cggaaatcag gaagtaagga agctggtaca tgaattaatt aatgcattgt tcgcacaaaa
    36301 taaaaatcct ctgagccgag aagtagtttc ggtccgtttg atcaatgagc tcgttcaatt
    36361 agggcagtta ttgggagtag attcaaaaaa ttttgataac atgaaacaaa tctatgtaaa
    36421 agcattgaat aattatcatc ctaaagagct agaaaaactt gttttagaac aattagtctt
    36481 gccaatcaca gaatgttctc aagcaatcac tgatcatgaa tttaaaaatc tttcggagaa
    36541 tatcatgcac atcgtctata cagaatatga ccgtgatcta tctctagatg tgattgctga
    36601 taggttgcat tataacccta attatttaag caatgtattt aaaaaggaaa caggagaaaa
    36661 atttggtgat ttcgtccaaa atcatcgtct agaggtagcg aaaaagtggt tgaaagaaac
    36721 aaatctctct gtaaaagaga ttgcagaaag actgcaatat cgaaatccgc aaaattttat
    36781 tcgtttcttc aaaaagaaag aatcgatcac tccgggagaa tatcgaaaaa aatatttgtg
    36841 aatcaagagg ctgtttttct tgtgatctga ttcaagcaat aaaagaggac agcgaaaaac
    36901 agcatttcat gttttgtgct ggctgatttt attgccgcag aatcaacaag ataaacgccg
    36961 tttaatcaag aggctgtttt tcttgtgatc tgattcaagc aataaaagaa aggagaataa
    37021 ccttagtatt tgactttatg gatagttaaa tttcaaggct aagcacgcaa aaaggactgt
    37081 gactaaagct ttgtcacagt cctttttgcg aataaacggt gttcaagaga cagcttataa
    37141 acaataaggc tgaacgtctc tttcacaacc tccattttat gtcccataca caatcaacct
    37201 ttaacagaac ctacgagtgc gcctttgaca aagtatttct gaacaaaagg ataagcaatc
    37261 agcataggga ctgtggcaat aacgataaca gccattttaa ctgtttgtgc tggtggaatc
    37321 acatcaacgg cactactttc tgcttgcatc ccgcttgata caatgacgat ctgacgtaaa
    37381 aggatctgta tcggccattt agatgaatcg ttgatataca aaatagcggt catataagtg
    37441 ttccagtaag taacagcata aaaaagtgag atagtagcaa tagacggtaa cgctaatggt
    37501 agcatgattt tgaaaaatac accaaaatct gtgcaaccat ccatttttgc cgattcttcc
    37561 aaactatccg gcaaggcttg gaaaaagttt ctcataatga tcatgttcat tgcattaaca
    37621 gctacaggta agatcattgc ccaataagaa ttgaccagtc ccagagaacg aacaaccaag
    37681 aatgtgggaa tcattccccc gctgaacaac atagaaaata caacaagaaa gttgacgaat
    37741 cctcgaccaa acaagtactt tctggaaagc ccgtatgcca tcagtgaagt tgcgcacatg
    37801 ctgacgatcg ttccaacccc ggtaacgcca atcgaaacag caagtgctct aaaaatagtt
    37861 ggcgtagata gaatatagcg atacgcgtct agagaaaagg tagtagggaa aaggataaat
    37921 tttttcgcga cgacttcttg tgtagaagcg aaagatgtag cgatgatatt taaaaaaggc
    37981 acgatacaga tcaaagctag caagatcaga aacgtattgt tgaaaaatgt aaaacttctt
    38041 tctcctatag agactctctt tttcatatag ataggctcct cctcaaaata aattaaatgt
    38101 tctgtttcta tgtattcaat ttatcatcgg tgaaagatta cgtatataat catttcctac
    38161 gttaagtgcc agatagaaaa aaatagaata gttactaggc gtatcttatt tgtgaagaaa
    38221 tacactttta tattttgcga gtataaaaat cattgtcgtg taagcgtttt tacgtgcgat
    38281 aaaaatgatt atatacgtaa ttcggatagt tatctatcat aaagtcataa gcagcaaaca
    38341 ttcataaaac taaggagggg cttatggaaa gtgtaaagaa acaagaacca tttcaactga
    38401 cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa aataattgca ggaatcaaaa
    38461 cgaataaatt tttatacgtg atgattctgc cgggggtcct ttatttcctg atctttaggt
    38521 atcttcctat gggagggctc gttattgctt ttcaagatta tcaaccattt ttagggataa
    38581 tgggcagtcc ttgggtggga ttcaaacatt tcattcgttt gtttacagag ccgactttct
    38641 ttactttatt aaggaatact ttgatcttgt tcgggttaaa tatcttcgtg ttttttccga
    38701 tgccaattat tttgtcccta ctattaaacg aagtaacgaa caagcgactg aaaagtggga
    38761 ttcaaacgat catttatatc cctcatttca tgtcttgggt aatcatcgtt tcgattactt
    38821 atgtattttt gaacgttgac ggaggggtat tgaatgaatt ccttgcaacg atgggattcg
    38881 aaaaaatcag ttttctgaca tctcctgagt ggacacgaac agtctatatt ggacaagtaa
    38941 tctggaaaga gttgggctgg tctacaatca tctacctttc agcaatcact gcagtagata
    39001 cacagttata tgaagcagct gaaatggatg gcgcaggacg cttgagaaaa acatggcatg
    39061 ttacattgcc ggcgattcgt ccagtaatca ttaccttgct gattctaaaa atcggtcaga
    39121 cactggactt aggtttcgag catatgtact tgttattgaa ttcattgaat cgaagtgttg
    39181 cagaaatttt cgacacctac atttatacag ctggattgaa gaatggacaa ttaagtttca
    39241 gtacagcaat gggattgttt aaaggggtcg taggattatt catggtaatt ttagctaaca
    39301 agatcgccaa aaagctagga gaagacggcg tttactaatt tagaaagaga ggaaaagaca
    39361 tgctgcagtc attagataaa ataaatggaa ggctaaatcg tattttacag ttggtttatt
    39421 tgaattttct gtggggtggt ttagtcattg caggtctggg ggtttttact tttggtcctg
    39481 catcttatgc aatggttgca gtgattagaa aatggctgcg cacaaaagaa acatttcctt
    39541 tgacgaaaac gtacttctca tattttaaag aaaactatcg tgaaaccttg ctgatgagct
    39601 ggatatacgg cgtcatcgga gttgttttac ttgtggatct ttctttcata cacaattggt
    39661 atcttagaat cgctttgtat attgctgcgt tcttgtattt gctctcagca ctctatattt
    39721 ttccaattat ggcacattat gattggaaag gatatttttt caaaatgaaa atggcatttt
    39781 tgttcggatt ctctcatctt cattattcat tagtattaat actaagtctg gtgggtattt
    39841 acctgatgat catccaaaca atgccaggtg ttttaacctt tatcggtggc agtctcttct
    39901 tttccgcagt cacgtggaca gcaaattcta tattttgcca tttggaatgg aagaactcat
    39961 atacagaaaa aacagaagaa aacaaaaata tcattaaaac agtgaaggag aaaatttatg
    40021 aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag gaattttagc acttgccgca
    40081 tgtggcaaca gttcaaacaa cagttcttca gaaacagaag atgggaaaac caaaatcact
    40141 tggttgaata ttttacacca tgcttcccca ccaactgaca caatcgtgaa tctaatagaa
    40201 gaaaaaaccg atacggaact tacattcaat tggattcctg atgcttcaaa agaagaaaga
    40261 ctgacaactg cattggcttc tgacgaacta ggagatatcg ttacacttca aattgctatg
    40321 ctgaaaaact cttctgtccg aagtgcgttg aaatcaggcg tattctggga tgtatcggaa
    40381 tatttaaaag actataaaaa cttaagccaa atctctgagg atagaatcga agctgcaagt
    40441 atcgacggag cattgtatgg tgtgccaatg caaaaggact atgctcgtgc tggtttagtc
    40501 attcgaaaag actggttgga taatcttgga ttagatgttc caactacgat ggatgaactt
    40561 tacgaagtag ctaaacaatt tacagaaaac gatccagatg gaaatggtgt ggatgataca
    40621 gttggttttg gtgatcgtgc gcctagtgat attcgttata ccagctttaa gctgttcact
    40681 tcttattttg gagcaccgaa tggctgggct gtagatgata atggcaaatt tactccagaa
    40741 tttgatacag aagaatacat gaaagcgttg aacttctcaa gagatctatt tgagaatggc
    40801 tatttggcac aagattttgc ggttactcaa aagactgacc agcaagaaca atttgcacaa
    40861 ggaaaaacag gaatctacac tgggatggtg gatatcaaga acctgaagac aatgggacaa
    40921 ggtattcaag atgatatgga attagtccct gtaaacaaga tttcagatgg tgaaggcggc
    40981 ggttatcatg tttggtcaga aggaaatggt attggtggtt tgttagcatt ccctaaatca
    41041 caagtaaaag acgaagcaca attgaagaaa ttactgcaat ttgttgatga tcttctagaa
    41101 gaagatatgt ataaattgat gactggtggt attgagggaa cacattatga attgaatgat
    41161 gatggttcta ttgaatttat cgatatggac ctttggcaac aagaagtgca accgctatca
    41221 agttctcgcc ctagtgaagt gacgttctct ttcaaagatg cagatcctga aaaagaattg
    41281 tcgaatcaat tgatcaaaga aaacgaagaa ttcgctgtaa tggatccttc tgtaccattg
    41341 gattctgcaa cgaacaatga agtagggtcg gaaatcgaaa aaatcgttgt tgatgctaca
    41401 gtgcaataca tcatgggaca gcttgacgaa gacggattca agaaagctgt agaagactgg
    41461 aaagcacaag gtggcgaccg aatcacacaa gagtacgagg aagcttacaa agccgcacaa
    41521 tagtatagat gattgggaca aatacttctt ggtatttgtc ccaaatccta ttttaacttt
    41581 taaaggagag taaataatgg aaaactggtc aaagaaatta gcaaaaagca tcatggaaag
    41641 aacacctcgc ttatatgaag aaaagtggta taaaggaaaa tggtcttatg actatggcgt
    41701 cgtacttaaa gggttccaat tactttggga acaaacccaa gaaaaaattt attttgattt
    41761 cataaaagac aatatcgact atttcgtgca agaagatgga acaatccgtg gctacagcgt
    41821 tgaagaatac aatatcgacc atgtaaatac cggaaagctt ttctttttat tgtacaaaga
    41881 aacaggcgaa gaaaaatata aaaaagcagc agaactcctt tcccagcagt tggcaaacca
    41941 tccgcgtaca tccgaagggg ctttttggca taaagaaatt tatccttatc aaatttggtt
    42001 agatggatta tatatgggct caccatttta tgctgaattt atccagaatt ttagtgaaga
    42061 aaaagattac caagatgtct tgagacagtt tgaaatcagc tataaacatt tattcgatcc
    42121 aaaaacaaat ttattaatcc atgcatggga tgaaaagaaa gtccaaccat gggctgaccc
    42181 agaaacaggt ttatctcatc atttctggag tcgatcgatt ggctggtatc tgatggctgc
    42241 ggcagatacc tatgaattgc ttcatgcgga aacggattgt tctttgctga aggaaatttt
    42301 agaaaagaca ttgaccgcac tacttccgta tcaagaagaa aacagtggga catggtatca
    42361 agtaacaaca gaaacaaaac ggaaaggaaa ttatctggaa gcttctggca gcagtatgtt
    42421 tgtttacgca atggctaaag ggatacgctt aggcttgctg aacaaagaag agtggctgcc
    42481 gcaagtgaaa aaatctcatc aaggactact cgatgaattt gttcttgaaa caaaagaagg
    42541 ctggttgaac ttgaacaaaa actgtcaagt agcagggtta ggcggagcag aacaacgaga
    42601 tggttcatac gcttactaca tcagtgagcc gattatttgt aatgatcaaa aaggtgtcgg
    42661 tgcattcttg caagcattaa tcgaagtgga ggcattataa atatgtatga tattttgaca
    42721 tttggcgcat caattgatga attgaatact gaagcaatac aacaagcgat tgatgcagca
    42781 gcaagcgcag gaggcgggac cgtagttgtg ccagcaggtg aatttttaac aggggcttta
    42841 tttttaaaaa gcaatgtaga acttcatttg agcgcagggg cggttttgaa attctctgac
    42901 gatccaaaag attatcctat tgttcattca cgttgggaag gagtccatcg aaaagtctat
    42961 gcttcctgta tctatgctca aaatgtagag aacatctctg tcactggttt tggtacatta
    43021 gatggaaatg gaaaaaaatg gtggcatact ttccgaaacg aaccagataa tctggcttat
    43081 cctcgtccta aattgatgag tttccataac tgccatcgaa tcactgtgaa agatatcaaa
    43141 ctgatccagt caccaagctg gacgatcaac ccaattcttt gtagcaacgc gacgtttgac
    43201 aacttgacga ttttgaaccc agcagattca ccaaatacag acggaatcga tccagaatcc
    43261 tgcaaaaatg tgcgtattag taattgccat attgacgtgg gagatgactg tatcgcaatc
    43321 aaagcaggga ctgaagatac gtacgaaagg attgcttgtg aaaatatcac gatcacaaat
    43381 tgtacgatgg tccatggaca cggaggggtc gtgttaggaa gtgaaatgag tggtagtata
    43441 cgtaatatta ccatttcaaa ttgtatcttc caagaaactg atcgaggaat acgattgaaa
    43501 tctagacgag gtcgaggcgg gatcgttgaa gacattcgag tatccaatat agtcatggat
    43561 aatgtgatgt gtccatttat actgaatctt tattatttct gcggtccgcg aggaaaagaa
    43621 ccatatgtat gggaaaaaac ggcttatccg atagatgagc ggacaccagc attcagaagg
    43681 atccattttt cgaatatcac ggcaaggaat gtccatgctt ctgcaggatt tatttatgga
    43741 ttagcagaac agtttataca agaaatcaca tttgataata ttgatgtttc aatggcccaa
    43801 gatgcagttc ccggtgtccc tgctatgatg acagggatag aggaaatgag caagcaagga
    43861 ttttacattg gttttgcgga taaggttacc tttaatcaaa tgacgattga gaatcatgaa
    43921 ggaccagcat ttctattaga aaatacgaaa gaaataagta ttgagaaatt ccggtcaact
    43981 caatcggaaa atatggatga gctggttgtt cgtaaaaact ttgcttatgc agttgaatga
    44041 ctttttaggt gatccatcta gcagacttac tcctagtgga aagcgattat acagagaaaa
    44101 acaaaatggg tttatgcttg aatattatca actgtactta aatgaattgg aaacagtacc
    44161 agcactttat gcttatccgg agaaagaagg accgtttccg acggtccttt acctgcattc
    44221 tcacggcgga gatttttcag tcgggaaaag tgaattgatc catggtgctc cttacttagc
    44281 ttctccatct tttgcagaag tactgacggg aatgggatat gctgtttggt cgattgatgc
    44341 ttggggattt gaagaacgcg gaggtatttc cgaaagtgag ttgttcaaac aatttttatt
    44401 aacaggaaaa acgttatggg gaatgcgtat ttatgatgtg atcagcttga tcgattattt
    44461 ggagacaagg gaagacacag atactcaacg gattgcaacg attggtcttt ctatgggagg
    44521 cttattaagc tggtgggtgg cggcattgga ttctcgtgtc aaagtttgta ttgatctagc
    44581 agcacaggtg gacatcgaaa cacttttgct acatcgtcgc ctagatcatc atgggtttta
    44641 ttactatgtg ccaaacctac tgacagctta tacaacgtta gcaatccaag aaaaaatcgc
    44701 tccgcgccct cgtcttagct tagttggaaa aaacgatacg atgtgcttgg acgaaggcgt
    44761 actgaagctg agaaaagggc tggacaaaaa gtatcaatca atgggtagtc cagaaaactt
    44821 ttcttctttt tctttaacag gcggtcatat ggaaacacaa gaaatgagaa acatttggaa
    44881 acaattcata aaggagcatt tatgaggatg aaagacaaac tgataattgg aaaaaattct
    44941 atagataccc cgacggattt tgaaactatc caagaaggaa tcgattattt ggcagacctg
    45001 ccggatagcc atccaaaggt cttgatagta aaagaaggaa gatatgatga atatatagaa
    45061 agcagattat ccaattttaa acttgtggga acaggaacag tagtgatctc cggtaatcgt
    45121 tttgcaaaac aggagtatct tgacggtagc cagttaggga ctttccgaag tgctactttc
    45181 ttcttagaag gatcgaatgt cgtattagaa aatcttcaca tcgaaaatac agcaggtcca
    45241 ggagaaaaag taggtcaagc tgttgcttta ttcaattcag gagatcgcgt gttgttaaaa
    45301 aactgtcgac taactggtta tcaggatact ttatgcacgg gtccgttgcc tgacttgcaa
    45361 aaagatggta cacccttttg tacaccggct tctgtcaatc ctgattattg tcgtcagacc
    45421 tatgaatatt gctggattga aggaacggtg gatttcatat ttggtggggc agatgcaatt
    45481 tttcgccagt gccgtatcca tagcttagcc agtaaaaatc ctggatatat cacagcagca
    45541 tctacgccaa aaaaacaaaa atcagggtat cgttttgaaa gctgtacctt gactgcagag
    45601 cctgatacta gaaacgtcta tttaggaaga ccgtggcgtc cgtatgcaaa tgtcgtgttt
    45661 gaagcatgta agataggtgg gcatgttcac ccaactggtt gggataattg ggatgacaag
    45721 aaaaatgaac agactgccaa attctctgaa cgaaataaca attatgaaac accaatcaaa
    45781 agacctgaat ggatccattt agaaaagcag ggggttctga aatgaaaaga aaatgggaac
    45841 tattgctagg aatgatcggt ggcagcctat ctctgatttt ctttggcggg ttagcagtta
    45901 ctttaagtaa tatgtctgcg tcagagttta aaaaaagtta tcagtcgctt gctgtcgatc
    45961 atccatcttt gtctttagaa aatacatttg agctattaca ggacatgact ggtctgtttg
    46021 ccgttgtatt gtttatttca cttgcttttt tagcagtagc actatttttg acggctaaag
    46081 gaaaatactt aacaactgcc actggcttgt attttataac aggtgttata ttgttggtcg
    46141 gcactcagtt tattgcgttt ccttttgcat ttttttattt tgccgctggg gcattcagtt
    46201 tgtatcgagt aagaataaga aaggaagcct gaatcatgca gactatcaaa gtaaaaaatc
    46261 ctattttgag agggttcaat ccagatccta atattttaag agcgaaagac cgatattaca
    46321 ttattgtctc ctcttttgaa tggcttccag gaattcgagt atatgagtcg aaaaatttag
    46381 tcaattggtc acatacatcg gatattttga ccactcaagt atctttgcaa ggaaatccta
    46441 caaacggaag catatgggca ccgcaaatca gttatgctga tggattgttc tatcttgttt
    46501 acacagatgt caaaagtgca ggccgtccgt ttaaagatgt ccacaattac ttgattactg
    46561 catctgagat cacaggacct tggtccgaac cgatttattt aaatagtaac ggctttgatc
    46621 cttctttgtt tcatgatttg gaaacaggaa aaaaatggct tgtcaatgaa ttatgggatt
    46681 atcgtctgga cacaccgaat aagtccagcg gtatcgtatt gcaagagtat gatgcagaga
    46741 agaagaagct tgatggtccc atctataaaa tatttgcagg tacagaatta gcgaaaacgg
    46801 aggcaccgca tttgtatttt cgtaacgggt attattattt gatgacggca gaaggaggta
    46861 caggaagagg acattcagtc acagtctgcc gagcaaagaa tattattggt ccttacgagc
    46921 ttgatcctgg ttttcctatg ctgacagcta gtgacaaacc tgcttctaca ttgcagtgta
    46981 caggacatgg gagcttagta caaaccccgt caggaaaatg gtacatgaca tatctttgta
    47041 ccagaccatt aaacggtgcg gctattttgg gaagagaaac cgctatccaa gaagtgtatt
    47101 ggacgtccga cgattggtta agactaaaag atagccagac agctccgatg gaagaaataa
    47161 tgattgagac gaatgaagag gtacaacaag aaagagcgta tcagtttatg gatacattcg
    47221 aaggagagct aaaaaagaac tggaatgcca gacgtatttt gccaagggct gattggtgtg
    47281 atacaaaaac tagacctggt tttttacgac tgattgccgg tgaatctctt caatctacct
    47341 ttggccatca cttattagct attcggcaaa cggattttac ttttgatgcg gagacagcat
    47401 ttgaatatac acctaataat tttaatcaga tggctggact ggtattgtac ctaaacgaat
    47461 ccaattatat ttattgctat gttacttggg atgaaaacaa gggaaaatgt cttcgtatga
    47521 tagaatcaga aaacggagtt gcacaaatag aagagtggag gattccactc acagaaaaga
    47581 agactttctt gaaagtagcc gtaaagaaag agacagcaca gttttatttg aaagacacac
    47641 catctgaaga atggaaggaa gtaggtctac aaaaaaacat gtgtatactc tcaggtggat
    47701 ttacggggaa ttttatcggg atagtcgtac atgatttaaa tagaaaaaga ggaagttatg
    47761 ctgactttga attttttaag tatgatggaa aagatcatga atgatctatc tctctaataa
    47821 atagagattc actaacttgt ttttgctagt ta
//
LOCUS       DADJYG010000007        46333 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.007, whole genome shotgun sequence.
ACCESSION   DADJYG010000007 DADJYG010000000
VERSION     DADJYG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.007"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            61..879
                     /gene="purR"
                     /locus_tag="JZ382_000323"
     CDS             61..879
                     /gene="purR"
                     /locus_tag="JZ382_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAY2426536.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1352..2725
                     /gene="glmU"
                     /locus_tag="JZ382_000324"
     CDS             1352..2725
                     /gene="glmU"
                     /locus_tag="JZ382_000324"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY2426537.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDYIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGNTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGAHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2819..3790
                     /locus_tag="JZ382_000325"
     CDS             2819..3790
                     /locus_tag="JZ382_000325"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAY2426538.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3796..3898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3964..4533
                     /gene="thiT"
                     /locus_tag="JZ382_000326"
     CDS             3964..4533
                     /gene="thiT"
                     /locus_tag="JZ382_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAY2426539.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKSY
                     ADGNTKQIRKNIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTMIVLLGIQQTIPSLFVPDKRQNL"
     gene            complement(4615..5388)
                     /locus_tag="JZ382_000327"
     CDS             complement(4615..5388)
                     /locus_tag="JZ382_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426540.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDDEYRIPIYVIEDYIW
                     KLKGTRYREEEVVATLIYSVNDRRWQLVEDAGLSYLPLK"
     gene            5645..7345
                     /locus_tag="JZ382_000328"
     CDS             5645..7345
                     /locus_tag="JZ382_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY2426541.1"
                     /translation="MEIKKVYFSQKMSLYSRINQAKQPLPFFQSHQESREAFWRSAKK
                     QCAFEAVSKKYTIYFTIDKSREDVIFNNLLIEGETFKWQKLFQHMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTMKLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     NIRFEIITGTSVGALNGALILQGDMEKAVGLWKKLSTRQVLALPEMAAVEDLRERFYQ
                     ERRQMTKTALVEGGVSSAPLEKLVKENLDLSLLKYNSKIRLYTVSTKLPELAEVVTAI
                     QQTETEEIPDWILASASFYPAMAYRKIGKNKYADGGFRNKIPIDIAINEGATEAFVVD
                     VQGPGPVKRIRVPDTFIHWRCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGCYYG
                     NWYTFDSVKQAGTCWRRFLSYLIKELQLELSFFKTIKFWQKLRNIYKNRVEPETCGLA
                     MLELLAKKHFLLPNKVYEVGWMFQMICQQDRKSMPDDLLAQVGQLSTEEWRRYRKYQQ
                     KIQNERTKAVYFEKLLQEKCKDRLQRELLEQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7315..7533
                     /locus_tag="JZ382_000329"
     CDS             7315..7533
                     /locus_tag="JZ382_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426542.1"
                     /translation="MAQEFSYEIVEEIAVLSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7553..8443
                     /locus_tag="JZ382_000330"
     CDS             7553..8443
                     /locus_tag="JZ382_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY2426543.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFI
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSIRRLAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ASVLKKNTEEFKQVLKKLNSFVDVYEQEFQLRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8461..8745
                     /locus_tag="JZ382_000331"
     CDS             8461..8745
                     /locus_tag="JZ382_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426544.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9354..9968
                     /gene="gmk"
                     /locus_tag="JZ382_000332"
     CDS             9354..9968
                     /gene="gmk"
                     /locus_tag="JZ382_000332"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY2426545.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKHIIASEHFRVDRVIGKYIKMLKEM"
     gene            9973..10278
                     /locus_tag="JZ382_000333"
     CDS             9973..10278
                     /locus_tag="JZ382_000333"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY2426546.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10531..12939
                     /gene="priA"
                     /locus_tag="JZ382_000334"
     CDS             10531..12939
                     /gene="priA"
                     /locus_tag="JZ382_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY2426547.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEIQALFGQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKDKEISTAVLNQGKEKGWLEFVESERYRDPYQDRVFDQTTALELNTEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQSIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     VAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQDRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCRGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKPGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12954..13445
                     /gene="def"
                     /locus_tag="JZ382_000335"
     CDS             12954..13445
                     /gene="def"
                     /locus_tag="JZ382_000335"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY2426548.1"
                     /translation="MRYPILIHPNDKLKIPAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESMDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMVQQIPEEELEEYMEEH
                     LDD"
     gene            13438..14376
                     /locus_tag="JZ382_000336"
     CDS             13438..14376
                     /locus_tag="JZ382_000336"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY2426549.1"
                     /translation="MTKLVFMGTPTFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELAPDLIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEEETGVTIMEMIKKMDAGGIYAQESMPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILNGQKPVPQDESKATFSPNITREQEAVDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGTRWKLLSVEVLAESTDEAPGTIIRKDKKNLWIACGNQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14376..15734
                     /gene="rsmB"
                     /locus_tag="JZ382_000337"
     CDS             14376..15734
                     /gene="rsmB"
                     /locus_tag="JZ382_000337"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY2426550.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVASLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEISAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVIALDVHEHKVKLIEENATRLGVSSAVYAQKMDAREVSKQFEPEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPALKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIVEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15747..16487
                     /locus_tag="JZ382_000338"
     CDS             15747..16487
                     /locus_tag="JZ382_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAY2426551.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEEQEAADDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDCV"
     gene            16484..18553
                     /gene="pknB"
                     /locus_tag="JZ382_000339"
     CDS             16484..18553
                     /gene="pknB"
                     /locus_tag="JZ382_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAY2426552.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHAMDNETKVITPLAADTPMPDSFKSMPLPKDEPRNETLPVEEEDLPDPKK
                     KGQKKWWLILLAVLAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     SFVVSQGTEPKLNDISGYTKSEAQSYLASIGAEYIGHETSEYSSTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGSDPVSSSSEKDSSDSSSSSSSSSSTSSSSKEESSSTTSSSD
                     STSESSK"
     gene            18837..19736
                     /gene="rsgA"
                     /locus_tag="JZ382_000340"
     CDS             18837..19736
                     /gene="rsgA"
                     /locus_tag="JZ382_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAY2426553.1"
                     /translation="MALIKGQIRKAISGFYYVYADGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPLIFLTKTDLSDDPKKIEEIKQTYTKIGYPVIVPNHAGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELLPIYDGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASAHCKFRECMHAKEPGCEVKQRVETGDIAQTRYVN
                     YLQFLQEIEKRKPMYTKKEGKRK"
     gene            19749..20399
                     /locus_tag="JZ382_000341"
     CDS             19749..20399
                     /locus_tag="JZ382_000341"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY2426554.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKIAELADL
                     RKENNWSYAIEVDGGIVPETAKVCKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20400..21038
                     /locus_tag="JZ382_000342"
     CDS             20400..21038
                     /locus_tag="JZ382_000342"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAY2426555.1"
                     /translation="MKILLAAGGNPENWPQPLLTKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQEEVFAKAKEYARSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGLEPRFKPFAHQLIIRDRQNYLAYYLPGKHIVRQVPSMKYLAYCCL
                     TPVSDLTLKGSKYVLENKAVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            21194..22048
                     /gene="mreC"
                     /locus_tag="JZ382_000343"
     CDS             21194..22048
                     /gene="mreC"
                     /locus_tag="JZ382_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY2426556.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHSLFSTYAENERLKEKIDSYDDVAQQNKNYERE
                     IESLKKELDLNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            22052..22561
                     /gene="mreD"
                     /locus_tag="JZ382_000344"
     CDS             22052..22561
                     /gene="mreD"
                     /locus_tag="JZ382_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY2426557.1"
                     /translation="MLKKETMKYYLPIVLFFLMLLDGHLTRMLGEWSKGSYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAVYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            22816..24372
                     /locus_tag="JZ382_000345"
     CDS             22816..24372
                     /locus_tag="JZ382_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426558.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAS
                     EESSAAQSSTTEESSSAAQSSTTEESTTAPESSTTEESTTAPESSTTEESTTVPESST
                     TEESTTVPESSTTEESTTVPETSTEESTTPAPTTPSTDQSVDPGNSTGSNATNNTTNT
                     TPTPTPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYLQVTGRDIGGW
                     TVPQESAGTKISVSQAKAGDLLFWGSPGGTYHVAIALGGGQYIHAPQPGESVKVGSVQ
                     WFAPDFAVSM"
     gene            complement(24440..26239)
                     /locus_tag="JZ382_000346"
     CDS             complement(24440..26239)
                     /locus_tag="JZ382_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAY2426559.1"
                     /translation="MSYSLNWDLDSIFPGGSHSDALNQRMKQLEDQTNEYYQRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCNSYITALLSANVNDSDAKILSGKLYAILPLLQ
                     SAETVLSKKFAEISDNDWNQMLSQGSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDRDPKIREALFAAWEKA
                     WKEKAPLFTDTLNHLDGFRLSNYELHGITDFLQKPLEYNRLKKETLSVMWETIQKNKQ
                     PLVDYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKAAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVMEDIQTFMELTEEFI"
     gene            26361..26867
                     /locus_tag="JZ382_000347"
     CDS             26361..26867
                     /locus_tag="JZ382_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAY2426560.1"
                     /translation="MNSSKKQLKNGNIYLVIALIVVAILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSIVSIDHLGYAKFVEFFLRKGAHFGTYFVLGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMLTGGRTPMFQDVMLDSIGALTAVVLCVL
                     FATFKKRL"
     gene            26946..27668
                     /locus_tag="JZ382_000348"
     CDS             26946..27668
                     /locus_tag="JZ382_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY2426561.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIVIERDGLAVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQREQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(27750..29147)
                     /locus_tag="JZ382_000349"
     CDS             complement(27750..29147)
                     /locus_tag="JZ382_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAY2426562.1"
                     /translation="MTRSSHRKKSKHVALLSALGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPISQDHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKSGDSFVIKPEVNGNTINVDAAVKQVKSAVNSGKDTIELTEFKEKPKVTSGDSSLKE
                     QLESMNAIAKVKATYSINGETFQIPSSDIMSWLTYDGKVDLDTEQVRQYVTDLGTKYN
                     TSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILTGQDFTRSPIVQGGTTA
                     DHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPSTPTPAGVFYVWNKEEDATL
                     KGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVMK
                     ELFGMVEKGTPVLVF"
     gene            complement(29317..30291)
                     /locus_tag="JZ382_000350"
     CDS             complement(29317..30291)
                     /locus_tag="JZ382_000350"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAY2426563.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            30644..31204
                     /locus_tag="JZ382_000351"
     CDS             30644..31204
                     /locus_tag="JZ382_000351"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY2426564.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDQIAREHQAAFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SIFQSVKAVEYLIEGHSFVDAMNQFN"
     gene            31221..34742
                     /gene="mfd"
                     /locus_tag="JZ382_000352"
     CDS             31221..34742
                     /gene="mfd"
                     /locus_tag="JZ382_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY2426565.1"
                     /translation="MDIIQLVGKDPQVKEWISNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVAALHKRLPNKKTWLQAQLNWHVGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIVDIYPLTTNYPIRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQMTSSWQAG
                     IPTEEAKFYSDLLYQEFVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWHTQKIS
                     ELRVFSEQSFSADVQRIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKERIRKAEEMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEPHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            34757..36352
                     /locus_tag="JZ382_000353"
     CDS             34757..36352
                     /locus_tag="JZ382_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAY2426566.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQSLYPLVFWTGVFLWLF
                     VFSFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     LVRVSVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGVLSFR
                     RFPLFKQPPKSLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLIISLLCMTAVLLLYYGVLNIVFGPVTHRSIAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            36363..36632
                     /locus_tag="JZ382_000354"
     CDS             36363..36632
                     /locus_tag="JZ382_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAY2426567.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            36700..37125
                     /locus_tag="JZ382_000355"
     CDS             36700..37125
                     /locus_tag="JZ382_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAY2426568.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            37171..37641
                     /locus_tag="JZ382_000356"
     CDS             37171..37641
                     /locus_tag="JZ382_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAY2426569.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPVESGEESRPKKFQQKDSRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            37761..39206
                     /gene="tilS"
                     /locus_tag="JZ382_000357"
     CDS             37761..39206
                     /gene="tilS"
                     /locus_tag="JZ382_000357"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY2426570.1"
                     /translation="MNVEKDGKKVPLLQQSFIRHGNEQGFWQKESRILAAVSGGVDSM
                     VLLHLLLQAQRQIGFSLGVVHINHQLREESAEEAAYLRSYCEKKNLPLYVSVWENPAK
                     TAIETAARNFRYQAFTRVMEEGQYDTLMTAHHGDDQIETVLMKIIRGGQLSTFSGIKG
                     VQPFATGRLVRPLLSFSKEELYAYAAESQLVYFEDQTNQLLDVQRNRLRHLVVPQLKQ
                     ENTQVMRHFQQFSQQIQWADQVIQKCMGQLIEKEVEQLKDRFQFSAEMIEKMEEAERY
                     YFFHSFFDKVFSETGVILKEQQMTSILDQWQQKKSQWQLSIGNNWVLKREYHFVILAK
                     KEKQATAQTEDQRLLLIPEQGIQLNETEWIGLVKPEHSEDIKTTDSWSLFSHDIWVPA
                     DVQLYVEKRKAGDRIQLTEKLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            39206..39751
                     /gene="hpt"
                     /locus_tag="JZ382_000358"
     CDS             39206..39751
                     /gene="hpt"
                     /locus_tag="JZ382_000358"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY2426571.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPTVYQSN"
     gene            39841..41952
                     /gene="hflB"
                     /locus_tag="JZ382_000359"
     CDS             39841..41952
                     /gene="hflB"
                     /locus_tag="JZ382_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY2426572.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGVWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHAKAHEIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELHEEN
                     KAEHDSDSQRSEDNHSDDQYK"
     gene            42170..43057
                     /gene="hslO"
                     /locus_tag="JZ382_000360"
     CDS             42170..43057
                     /gene="hslO"
                     /locus_tag="JZ382_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY2426573.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPAAGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIISGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLDTEELNVLEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELAGLKKEALGGI"
     gene            43058..44062
                     /gene="dusB"
                     /locus_tag="JZ382_000361"
     CDS             43058..44062
                     /gene="dusB"
                     /locus_tag="JZ382_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY2426574.1"
                     /translation="MEHKWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVDIPVTVKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEILKEVKKHLTIPFMGNGDVKTPEDAKRML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTENQQEINGLLDQFVEKAEKVLLKQTL
                     "
     gene            44173..45669
                     /gene="lysS"
                     /locus_tag="JZ382_000362"
     CDS             44173..45669
                     /gene="lysS"
                     /locus_tag="JZ382_000362"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY2426575.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWKEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT        15264 a         8436 c        10084 g        12549 t
ORIGIN      
        1 ttttgaagaa aaacttatat aatgtaaaac gataaaattt cttaaaagga gttggaatag
       61 gtgaaagtac gtcgcagtga acgtctggtt gacatgacac agtatttatt gaatcacccg
      121 catgaattga tttccttaac aagctttgcc gaacgttatg aatctgctaa atcttccatc
      181 agtgaagatt tgggaatcat caagaaaaca tttaaagaac gagggtatgg gaccttggaa
      241 acaatcccag gtgctgcagg tggtgtattg ttcatccctg aaatttcata tgaagaggcg
      301 aaaaaatata ttgaatctct agcagaacgt ctatcagaac aagatcgtct gcttccagga
      361 ggatatgttt atctctctga tttgttagga gaacctgaac tgctgcgtca agttggaaga
      421 atcattgctt ctaaatacct tggtgaaaaa atcgatgctg taatgactgt ggccacaaaa
      481 ggcgtaccta ttgcccaagc tgtgtcctat tatctgaatg caccattcgt tattgtacgc
      541 cgagactcaa aaataacaga agggtcaaca gtaagtgtca actatgtgtc cggttcttct
      601 gaaagaattg aaaaaatgga attgtctaag agaagcttga aacgtggttc acgtgtcctt
      661 gtagtggatg atttcatgaa aggcggcgga acagtcaacg gaatgaagag cttgatcgaa
      721 gaattcgaat cagaacttgt tggcatcact gtatttgccg aatcgaaatt caacggacat
      781 cgcgctatcg atgattatac gtcactgctt tatgtcaaag atgtagatac tcaaacgaaa
      841 aacatcacgg ttgtgccggg aaattatttt tcagaataag aggttgtgaa aaagccgctt
      901 agtttcgagt agtaagaaaa attttgtgaa aagggctttc cacatttttt cataaatttg
      961 gcttaatacc gagaaactgg cttttgaaca ccgtttatcg agaggttgtg aaatcagcgt
     1021 cctgaccgga gtataagaga aacaaaacga aaaaatagct cctcctattt tttgtttggt
     1081 tgagcttatt accgcagata taagcctacc gtttaactct aagtcacaag gagcaatttc
     1141 aaaaaacagc ttttcttgtt ttatgagtag tatgacttgt gaccgaggag ttggcaggag
     1201 agcatcggtt acccaataga taggcccttt atggtctatc tattttttta tccaaagaac
     1261 accgtgatga aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
     1321 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
     1381 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtctctgg
     1441 taaacctatg gtagaacata tcatcaatcg ggtcagtgaa acgaaaccag atgaagtgat
     1501 taccatcgtt ggacacggag cagaacaagt caaagcacaa ctaggagagc gcagtaaata
     1561 tgcgttacag gcggagcaat tgggaacagg acatgctgtt ttgcaagccg cttctttctt
     1621 ggaaggaaaa aaaggaacga cgttagttat cagtggcgat acaccgttat tgactactga
     1681 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcga caatcttgac
     1741 ggcacaagca gaaaacccaa caggatatgg tcggatcatt cgtgattata ttgggatcgt
     1801 agaaaaaatc gtggaacaaa aagacgcaac accagaagaa gcacttgtac aagaaatcaa
     1861 tacaggaaca tactgttttg acaacgaagc tttgttcgat gcgttaagca aagtcggaac
     1921 aaacaatgct caaggtgaat actatctgac cgatatcatc gaaatcttaa aagaagaagg
     1981 caatacagtt gctgcttatc aaacagatga tttcgaagaa tcaatgggag tcaatgatcg
     2041 aatcgcgtta gctaaagcaa atgagatcat gagaaagcgg atcaaccaga tgcatatggt
     2101 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagagattgg
     2161 tccagacaca ttgatcgaag caggtgtaca aatccaaggc aatacagtca tcggttctga
     2221 ttgtgtcatc ggagcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
     2281 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
     2341 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
     2401 gaaaaatgct caaatcggca aaggaacgaa agtcggtcac ttgacttatg ttggtgatgc
     2461 tacgttggga gaagagatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
     2521 gaacaaacat catacgactg tcggtgacca cagctttatt ggttctagca cgaatatcat
     2581 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
     2641 tattccagaa tatgcgttgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
     2701 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
     2761 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
     2821 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
     2881 agcagagaaa attgcagcag cagtcggtgt cgagttagga aaatcatctg ttactcaatt
     2941 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
     3001 gatccaatca acgagcagcc cagtcaatga taacttgatg gagttgctga ttatgattga
     3061 tgcactgaaa cgagcaagtg cgaaaacaat caacgtagtc atgccttatt atggttatgc
     3121 gcgtcaagat cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
     3181 gatccaaaaa gccggtgcaa cacgaatgct gactttggac cttcatgcag tccaaatcca
     3241 aggtttcttt gatattccag tcgatcattt gatgggtgca ccacttattg ctaattattt
     3301 cttggaaaaa ggaatcaaag gtgatgatgt tgtcgttgtt tcaccagacc acggtggcgt
     3361 tacacgagca cgtaaattag cggaattctt gaaatctccg attgcgatca tcgacaaacg
     3421 ccgtccaaaa gcaaatgttg cagaagtaat gaacatcatt ggacaagtca aaggtaaaac
     3481 ttgtgtactg atcgacgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
     3541 tttgaaagaa gctggtgcaa caagtgtgta tgcttcatgt acccatccag tattatctgg
     3601 tcctgcttta caaagaatag aagattcagc tatcgagcga ctagttgtca cagactcgat
     3661 ctatcttcca gaagatcgta aaatcgataa aatcgatgaa gtaagtgttg gcggattaat
     3721 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattat ttgaaacaaa
     3781 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
     3841 ctaaaccctt cgtacctgtt tcggttatgc gagcgtagga attgtgaaaa aacagctatt
     3901 tgaaattttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
     3961 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
     4021 ctgtcattga taccaatcca gattggcagc agtttttcta tttcattggg gcagattccg
     4081 ttgacgcttt ttgcattaag aagagggaca aaggcaggta tgctagcagg tttcatttgg
     4141 gggatcttac actttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
     4201 gagtatccga ttgcttatac ttttgcaggt gcagcagggc ttatggcttc aaatgtccaa
     4261 aaaagctatg cagatggaaa tacgaaacaa atcagaaaaa acatagtgtt tggtgcattg
     4321 attggcgcac tttccagata tttttggcat tttattgcgg gagttgtttt ctggggagcc
     4381 tatgcactat ggggaatgaa tccttggctc ttttctttcg taatgaatgg tgctagcggg
     4441 ttgagcacag cgattgtgac gatgatcgtt ttattaggga tccaacaaac gattccatca
     4501 ctttttgtgc cagataaacg acagaatctt taaataaaaa aggaaacaaa aaacttgaga
     4561 cagaatcatg tcctagtttc ttgtttcctt tttgtttttc ggcaagaaat agattcactt
     4621 caatggcaga taagaaagac cggcatcttc taccaattgc cagcgccgat cgttgacaga
     4681 gtagattaaa gtagcgacga cttcttcttc ccgataccga gtacctttca atttccaaat
     4741 atagtcctca atcacataaa taggaatacg gtattcgtca tcgatcaaac caacttgatc
     4801 aaaagaaaaa atgatccctt cctgcattaa attacgcatt tctttcaata attccacact
     4861 gattttttca acaggtgttt cctttttgaa aaaggatttt tttacattga tcttatctga
     4921 caggatagaa cttaatttac tagcaaaaat cctgtaaaat tcatctaaat agcgataata
     4981 aattcggttc aattgatcgt tcaggtcgaa gtacacgaaa ttattttgta gcttataaaa
     5041 aaatggtgag tggagatgtg ttttcatatg gccaaaatat aacaattctg aaatttctaa
     5101 aggtgtcaat tctttcagca tcgttaaatc agtaaagtca atccacttca attcatcgaa
     5161 tccacgcctg tttcgagaag aggaaaagaa acgctggata ttttctgtac cacggattac
     5221 tttcagccct gtatgagatt cgtattctcc agcagaagca gatggatcga gtaagagcaa
     5281 attttgagga aaatgcacga tactgtttgc aaaatcagta gcagtcagtc cttttgttaa
     5341 aacagcatta cttgtggtat caatatgtac ataaactgac tcagtcatca tgttcctcct
     5401 cttatttcct cttaatctct tttattttac aacatttgca taaaaaaagc ttgtttcttt
     5461 tattttaaaa ttccattaca aaatcacgtt ttcattgaaa atactctcat tttcaattaa
     5521 tttttgtatt ttttatttaa agaaggtttt atgaattgtt ttcttatgga atatccaaag
     5581 tcgaatacga tactcatgac cttttttcga cgtactggta tacttgaaga aaaaggagaa
     5641 agcgatggag ataaaaaaag tttacttttc acagaaaatg tcgctgtatt cgcgtatcaa
     5701 ccaagcaaaa caacctcttc cgttttttca aagccatcaa gaaagtagag aagcattttg
     5761 gcgttctgca aaaaagcaat gtgcctttga agcagtatca aaaaaatata ccatctattt
     5821 tactattgat aaaagtagag aagatgttat ttttaataac ttgctgatcg aaggcgaaac
     5881 ttttaaatgg caaaaacttt ttcagcatat ggaagcttgt gctcgttttt ttattaaaga
     5941 aaactgttgt tttttatttc aatctcctct atcagaagaa tggttgcgta tttttcataa
     6001 aaacggctat caaggaacca tgaaactaaa taaaaagctg gtctatcaca cagcacttgt
     6061 attaggagga gggggagcac acggtgccta tcagattggt gtatggcagg cgctgaagga
     6121 acataacatc cgctttgaaa tcatcactgg gacctcggta ggtgcgctga acggcgcatt
     6181 gattttacaa ggagacatgg aaaaagctgt gggtttatgg aaaaagctat caacaagaca
     6241 agtgcttgcg ttgccagaaa tggctgccgt tgaagacttg cgtgaacgtt tctatcaaga
     6301 aagacgccaa atgacgaaaa cggcactagt tgaaggtggg gtttcgtctg cgccattgga
     6361 aaagctggtc aaggaaaatt tagatttgtc tttgctcaaa tacaattcca aaatacgttt
     6421 atacacggtg tctactaaat tgccggaact agcggaagta gtgactgcta tccaacagac
     6481 agaaacagaa gaaataccag attggatact tgcctctgct tcattttatc ctgctatggc
     6541 ttaccgaaag atcggaaaaa ataaatacgc agacggtgga tttcgtaaca aaatccctat
     6601 tgacatcgcg attaacgaag gcgcaacaga ggcgtttgtc gtggatgtgc aaggaccagg
     6661 gccagtgaaa agaatccgtg tgccagatac gtttatccat tggagatgtc aaacattgtg
     6721 gacgcttggc agctttctgc tttttgatag ccaaagaaac caattaaatt tacaattagg
     6781 ctatctggaa atgaaaaaac gattaggctg ttattacggg aattggtata catttgattc
     6841 tgtcaaacaa gcaggaacat gctggcgtag atttttatct tacttaataa aagaacttca
     6901 gttagagcta tcttttttca aaaccataaa attttggcaa aaactgcgta atatatataa
     6961 gaacagggtc gaaccagaaa catgcggact ggcgatgctg gaacttttag caaaaaaaca
     7021 ttttttattg ccaaacaaag tctatgaggt aggttggatg tttcaaatga tctgccaaca
     7081 agatagaaaa agtatgccag acgatttatt ggcacaagtt ggtcaattaa gtacagaaga
     7141 atggcggagg tatcgaaaat atcagcagaa aatacaaaat gaacgcacga aagccgttta
     7201 ttttgaaaaa ctgctgcaag aaaaatgtaa ggatcgtttg cagcgtgaac tgcttgaaca
     7261 gccagttgac acattactga tcctatattt atattatctc aaggaggaac aaccatggca
     7321 caagaatttt catatgaaat cgtagaagaa atcgctgttt tatcagaaaa caacaaagga
     7381 tggcgtaaag agctgaactt agtcagctgg aatggtcgtc cgccgaaatt cgatcttcga
     7441 gattgggcac ccgaccatga aaagatgggg aaagggctga cactgacgaa cgaagagttt
     7501 gaacaactgc aaaaagcaat agaaaatatg taatgaggaa ggaccctgtc gaatgaaaag
     7561 tatgactggt tttggcaaag ccatccgcga aacaaaagca tatcagctag atatcgaaat
     7621 aaaaagtgtc aatcagcgtt ttctagatgt ccaaatacga agtcctaaaa tcctgaattt
     7681 tattgaaaac gattttcggc agcttatcaa acagcagtta agccgcggac ggatcgaggt
     7741 atttgtgaat ctagcttata ttggcaaaaa cgaaaagcag atcgtcatca attgggaatt
     7801 gattgattca cttatcaccc aattgacaga cgggattcaa caacgatacg ggagtcaaaa
     7861 tcaagtgtca attcgtcggc tggcggaacg cttcgcgctg gatgaaaact tcgtaacggt
     7921 agaagaaaaa ccggaagaga gcatggaaga attggcacag ttagctctag atacattgaa
     7981 aagtgcactt gattctattg aagccagtcg agaaaaagaa ggacaagcct tagcttcggt
     8041 gctgaaaaag aacacggaag aattcaaaca agtactaaaa aaactcaata gttttgtaga
     8101 cgtctatgaa caggagtttc aattaaggta tcaaaaaaag ctggaggaat ttcttggagc
     8161 gaaagtggat gagcaacggt tgctgacgga gttggctatc ttgctggaac gtggagatat
     8221 ccacgaggaa ctggatcgtt tggcaatcca tgtccaaaaa ctagatgaac ttatcgaggc
     8281 ccaatgtcct gtaggtcggg aattagattt tctgatccag gaaatgaatc gggaaatcaa
     8341 cacgatcggt tccaaatcaa acgcaatcga aattaaaaat caagtcgtcc aatccaagac
     8401 gatcttagaa aaaatcagag aacaggtcca aaatattgaa tgacgaaaga ggttgagaca
     8461 atgaaatact atgacgtaac gttccacgaa ttaagtggaa aaactgttgt taaacgggat
     8521 atcccttctg aaaaaaacgg ctttgacgta tggaaagatg cctgtgcaga ctataatgag
     8581 aacgaactgt ttattttgat caacgacggt gcttacgtga cgatgaatcg gaaatttatt
     8641 gttcgaatcg acacagaaga ggtagaagat ccgacagaaa aagcacgttc tcgaaaagat
     8701 gagatcatgg gtgtagtgaa tactttgtcc aatatgggct tttagcaagg atgctgaaca
     8761 aagaaataca ataaataatc agaattgaat ttcttcgctt agcttatttg ctctaggaat
     8821 acatttaaag gttgtgacaa aattcgtgtc acaacctttt tttattttga ataagtgatg
     8881 ttcaaaaacc attctctcgg tatggagcca aatttatgca aaaatatgag aagccatttt
     8941 tgtttggctg ttcttattac ttgatgcagg atagcttttt cacgacctct catacacggc
     9001 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaacatg agaagctgtt
     9061 tatgtttggt cgttcttatt acttgatgca ggacagcttt ttcacgacct ctcatacacg
     9121 gcgttcaaaa gcttgcatct acggtaataa gcccaaccaa acgaaaaaca tgagaagctg
     9181 tttatgtttg gtcgttctta ttacttgatg caggacagct ttttcacgac ctcttggtac
     9241 agggtagctt ttttaccaat ttctagctat aattaaagat aaccttgaaa gttgcagaaa
     9301 aaaagtgcaa aataaacgat aagtacaaaa agactagttg aaaggacgtc gtcatgtcag
     9361 agcgtggatt attaatcgtg ttgtctggtc catccggtgt aggaaaaggc acagtacgaa
     9421 aagcaatttt tgagagtgag gataatgatt ttcagtattc aatctcaatg actacccgta
     9481 aaatgcgtga aggggaggtc gaaggcgttg attatttctt ccgaagcaaa gaggagtttg
     9541 aatcaatgat cgaagctgga gagatgctgg aatacgcaga atatgtcggg aattattatg
     9601 gaacgccttt gtcatatgtc aacaagacat tggatgaagg aaaagatgtt ttcttggaga
     9661 ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc cgatggtgta ttcatttttt
     9721 tgactccgcc agatttagcg gaattaaaat cacgaatcgt aggccgagga acagatgcag
     9781 acgaagtaat cgaagaacga atgaaagttg caaaagaaga aatcgaaatg atggctcttt
     9841 acgactatgc tgttgtgaat gatgaagtgc cgctagcagt tgaacgaatc aaacacatta
     9901 ttgccagtga acattttcga gtagatcgcg taatcggtaa atatattaaa atgttaaagg
     9961 agatgtagtc ttatgatgtt aaaaccttca attgactcac tattagatcg tgtaaattca
    10021 aaatattctc tagtaatttt agcaagcaaa cgcgcacatg agttggatgc aagtgcacaa
    10081 cctacactag attcttttga ttcagtaaaa agcgttggtc aagctttaga agaaatcgat
    10141 gcagggaatg tagtcaatga tcctcatcct gaattaaaac gtgaacgttt gaagatggaa
    10201 gaagaggaac gtcaagctca aaaagagcgt gaacaacacg aattggaatc acgtatccgc
    10261 gaagagcaaa aaatgtaata aagaaaagta gagcgaacat ctaaaaagaa aagcaaaccc
    10321 agctgtttcc ttttcaaaca ttcgccactt attgtctggt agaaaaggct gtgtctgaac
    10381 tttttgacac agttcttttt tttcttatct gctgttaatt ttttgttagg atttggcgga
    10441 aaataagaca aatccagcga tctctagtaa aataggcaag gaactttttg tcgcttgata
    10501 tgaaaagaca aaaaaaggag gaaacgaacc atgacattaa cagctgaagt cattgtggac
    10561 gtacctacta tgcagaccga tcagccattc acctatctgg ttccctctga aatggaaaca
    10621 gctttgcaag tcgggatgcg ggtagaggtc ccttttggta atggcaatcg tcatgtccaa
    10681 ggatttgtta tggctatcca aaaatcagaa gaatcagcta acccatcgtt gaaggcagtg
    10741 atccgtttgc tggaccttgc tccggtggtc aatgaagaat tactttcatt agctgattac
    10801 atgaaaaaaa tcacttatgc attcaaaatc acttgtctac aaacgatgct tccgagtgtg
    10861 atgaaagcag aatatgataa attgatctat cctttggcag atacaccaga aattcaggca
    10921 ttgtttggtc aacgggaagt gatttcctgg aaagaagcag aagaagaagg cagcctttct
    10981 caattgattc gttggcgcca agagcaatta gtagatatca aatacgaagt ccatacacga
    11041 aataaagtaa aaacaattcg tttggtccgt tccctattaa ctgaaaagca aatcgaagaa
    11101 gaatgggcaa aattacggca aaatgccaag aaacaaaaag agcttctttt gtgtttatct
    11161 gaactgagcc aagaagaacc aatcgcttat ttcaaagata aagaaatcag tactgctgtc
    11221 ttgaatcaag gaaaagaaaa gggctggctg gagtttgtag aatcagaacg ttatcgcgac
    11281 ccttatcaag accgagtatt tgaccaaacg acggcattgg aactgaatac agaacaaaaa
    11341 aatgctgtcg aacaaatcat cacagcaggt cagcaacaaa aagatcaggt ctttttgcta
    11401 gagggaatca ccggtagtgg aaaaacagaa gtatatcttc aatcaatcgc tgatgttttg
    11461 tcagaaaata aaacggctat aatgttagtg cctgaaatcg cattgactcc gcaaatggtc
    11521 gagcgattca aaggacgctt cggtgaatct gttgcggtct tacatagcgg actttcccaa
    11581 ggtgaaaaat atgatgaatg gcgcaagatc gagcgtgaag aagcacaagt agtagtaggc
    11641 gcacgctctg ctatttttgc tccgctgaaa aatataggat tgatcattat cgatgaagaa
    11701 catgaatcaa gttataaaca agatgagacg ccgcgttatc atgcgagaga tttagctatt
    11761 tggcgcagta agtaccatca ttgcccaata gtccttggaa gtgcgacgcc ctctttggaa
    11821 tcaagagcac gagctcaaaa aggcgtttat cagttattgc aattgaatca ccgagccaaa
    11881 gtagctgcac aacttccagc tattgagatc gtagacatgc gtgaagagtt tcagaatcat
    11941 cggacctcta ctttttctgc taatttgcag gaaaagatac aagatcggtt agacaaaaaa
    12001 gaacaaactg tcctactgct caatcgtcga ggatattcgt cttttgtgat gtgcagagac
    12061 tgcggatttg ttctcccatg ccctaattgc gatatctctc ttaccttgca tatggataca
    12121 cgttcgatgc gctgtcatta ttgcggacac gaagaaccta ttcctaatcg ctgtcccaat
    12181 tgtaggggca acaaaatccg ttactatggg acaggcaccc aaaaagtaga agaggaactc
    12241 cgagaacttt atccgcaagc taggattttg cgtatggatg tagataccac ccgaagaaaa
    12301 ggggcacacg aacagatttt acaaaaattc ggtgcaaaag aagcagatat tttattaggg
    12361 acacaaatga tcgctaaagg cttggatttt ccagaagtca cattagttgg cgtgttgaat
    12421 gccgatacct ccctgaattt accggatttt cgttctagcg agcatacatt ccaattgctg
    12481 acccaagttt caggacgagc aggacgagcg gaaaaaccag gcgaagtcgt gatccaaacg
    12541 ttcaatcctc agcattacgc catagaattg gctaaaaaac aaaactatga acagttttat
    12601 cagcaggaaa tgcatgtacg gcatcgagga ggctaccctc cttattactt tactgtgaaa
    12661 ataactgcca gccatcctga agaacaagtg gcggcaaaga aaatctttca gattgccaat
    12721 caactaaaag aagtattaag tcctcaaagt ttgttactag gaccaacacc cagcatgatt
    12781 ttgagagtga aaaatcgata ctattatcaa ctgatcatca aatataaaca tgaaccgaat
    12841 ctgccccatg ttttggatga aatcttgaat ggaagccaga aagaacagcg gcaaggtctt
    12901 tttgtggcaa ttgacaacga accattaaac tttatttaag aaaggaccaa cttatgcgct
    12961 atcctatatt gatccaccct aatgataaat taaaaatacc tgctcagcca atcgacgtga
    13021 tcacggacga aacgattgct ttgttagaca acttatatga gacgatgatt gcgaatgacg
    13081 gaatagggat tgctgctcct caagtaggac aaaacaaaag aatcgctgtg atcgaagtag
    13141 atgaaggcga aaaattcgaa ctgataaacc cagaaatcat tgaagcaaaa ggtgaaagca
    13201 tggacgtaga aggctgtctc agtatcccgc atgtctatgg aacagtcaag cgagcagatg
    13261 aagtgactgt acgctattat gaccgcgatg gagaagagat agaagtgaca gctttcggct
    13321 atttagcccg tgccttccag catgaaatcg atcacttaga cggtattctg ttcattgaga
    13381 aaatggtaca gcagatcccg gaagaagaac ttgaagaata tatggaggaa catcttgatg
    13441 actaagttag tatttatggg cacacctact ttttctgtgc caattcttga agggctgata
    13501 gaatcaggct acgagatcca ggcagtagtc acacagcccg accgtccagt tgggcggaaa
    13561 cgagtaatta cgccgacacc ggtaaaagaa gctgctctaa agcacggtat ccgtgtgttg
    13621 caaccagaga aaatctccgg ttcgccagaa atggaagaaa tcattgaatt ggcacctgat
    13681 ttgatcgtca ctgcggcgtt tggccagttc ttgcctgaaa agttattgca agtgccaaaa
    13741 ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat atcggggagg tgcgcctgtt
    13801 cattatgcca tcatgaatgg ggaggaagaa actggtgtaa cgatcatgga aatgatcaaa
    13861 aaaatggatg caggcggaat ctatgcgcaa gaaagcatgc caatcactaa acaagatgat
    13921 gtggggacga tgtttgaaaa gctcagcctt ttaggaagaa agctattgtt agaaacattg
    13981 cctaatattt tgaatggaca aaaaccagtc cctcaagacg agtcaaaagc cactttttcg
    14041 ccaaacatca cacgtgaaca agaagcggtc gattggcata aaactgcaga agaaatcgac
    14101 aaccaagtac gcgggatgcg tccttggccg attgcattca cgacctatga aggtacacgc
    14161 tggaaattat tatcggttga agtactggca gaaagtacag acgaagcacc aggcacgatc
    14221 atccgaaaag acaaaaaaaa tctatggatt gcttgcggta accagaccgt gttagccatc
    14281 aatgaattac agccagctgg taaaggaaaa caagccgtac acgattttct taacggaagc
    14341 ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa aaaaatccct aaaaaaatga
    14401 aaaaatctgt acgatatatg gcattactga cattagagcg agtgaacaag ggcggcgcct
    14461 actcgaatct tttgctgaat gagatgatca aacaaagcga gctaaatcaa aaagacgtgg
    14521 gtctatttac agaattgatc tacggtacga ttagccatca gcgactattg gaattttacg
    14581 ttgcatcatt gatcgctaaa gcaaaaaaag tggatgactg ggtcaagacg cttctgtatc
    14641 tttctgtata tcaattagaa tttttagata aagtccctgc acatgctatt ttaaacgagg
    14701 cggtcgaaat cgcgaaagta aaaggaaatc ctggaacagg aaaatttgta aatggtgttt
    14761 tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg cattgctgat cctatcgaaa
    14821 gactttctgt caaaatcagc ctgcctctat ggatgacgaa acggttggtg gatcaaatcg
    14881 gttacgaaga aactgaaaag ctcggtttat cgctatatca gcctagccat gctagtgccc
    14941 gagtagatac acgccgactt tccagagatc aggccatcac tgttttgcaa gaagaagaga
    15001 tctcagctga aaaaagccaa gtcagtccat acggtatagt agcgaaaaaa gggcatttag
    15061 cttctagctc attgttccac gatggcgtca tgacgatcca agatgaaagt tcgatgcttg
    15121 tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt agatgcctgt gcagcacccg
    15181 gaggtaagac gacacatatt gcttcttttt tggatccttc atgcggtggg gaagtgattg
    15241 ctttagatgt acatgaacac aaagtcaaac tcattgaaga aaatgcaaca cgtctgggtg
    15301 tctcttctgc tgtttacgct caaaagatgg atgctagaga agtcagcaag cagtttgaac
    15361 cagagcagtt cgatcggatt ttagtagacg cgccttgttc aggtctaggg ctccttcggc
    15421 gaaaaccaga tatccgttat aaaaaacagc cggatgaatt agcgaattta ccaaaaatcc
    15481 aattggaaat tttagaaagc tgcgcaccag ctttaaagtc ttccggtatt ctgacttata
    15541 gtacttgtac gatcttaaaa gaagaaaacc aagacgttgt ttctgctttc ttggaaagac
    15601 accctgattt tgagaggatt gatgttttaa ccaatcctat gctacaatca tcaatcgtag
    15661 agaaaatgtt gactttgtat ccgcatcagt tttacacaga tggatttttc atttgttgtt
    15721 tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata tcaatcagat gtaggaagac
    15781 gacgtaacac caatcaagac tatgccagtg tatttactag ccgttcaggt ctcaagcttg
    15841 ctgtattagc agatgggatg ggtgggcacc gtgctggaga cattgctagt caaatggcgg
    15901 taacaaatct aggcgaagct tgggaagaac aagaagctgc cgacgatgaa aaaatcgccc
    15961 agtggttcat tcaatcgatt caagaggaaa atatgctgat ttatcagcga ggacaagaac
    16021 agccggaata taacggtatg ggaacaacaa tcgtagcggc agctctgtca gatgactggt
    16081 tcacgattgc gaatgttggc gacagcagag cgtatttgat tcgcgaagat aaaatcatgc
    16141 aattgacaga ggatcattct ttagttaacg aactagtcaa atcaggggaa atcagcgaag
    16201 aaatggcagt gaaccatcca agaaagaata ttttaacacg ttctgtcggc atgccaggaa
    16261 cagtcgaagt agatgtttct actcatcttt ggcagctaaa agatcgcttg ctgttatgtt
    16321 cagatggctt gacgaatatg ctatctgaag aagaaatagg aacgatcatt aatcaagaag
    16381 gcccgttatc ccataaagta actgagctga ttgatcgtgc caatgaagca ggcggagcgg
    16441 ataatatcac cgttcttctt atcgaatata aggaggactg tgtatgatcg agttgggaaa
    16501 aaaattaaat ggccgttatc aaatcacggg gaatatcggt agtggtggga tggctaatgt
    16561 atttttggcg cacgatttga tcttggatcg agatgtagca gtcaaagtct tacgattcga
    16621 ctttcaaaat gatcaaaccg cgattcgacg atttcagcga gaagctttag cagcaacaga
    16681 actcgttcat ccaaatattg tcagtgttta cgatgttggt gaagaagaca atatgcagta
    16741 tctagtgatg gaatacgtca aaggaatgga cttgaaacga tatatccaaa cacattatcc
    16801 tgttccttat gaaacagcag taaatattat gcagcagatt ctttctgcta tttctttagc
    16861 tcataaccat caaatcatcc accgagattt gaaaccccaa aacgtattga tcgataacga
    16921 aggtgtagta aaaatcacgg atttcgggat tgctattgct ttatctgaaa catcgattac
    16981 acagacgaat acgatgttgg gatctgttca ttacctatct cctgagcaag caagagggag
    17041 tatggcaacg aagcagtcag atatttatgc tttagggatc attctttatg agatgctgac
    17101 aggtagcgtg ccgtttgacg gcgaatctgc tgtgacgatt gctttaaaac atttccaaga
    17161 tgatcttcca tccataaagg cacttgatcc aaatgtaccg caagcactgg aaaatgtgat
    17221 tttacgggcg acggctaaag aacctgcaga ccgttacaag tcagccgaag aaatgtctga
    17281 tgatctttct acagtattat caccagctcg tgcaaacgaa gaaaaatggc aacctcatgc
    17341 gatggataac gaaacaaaag ttatcacacc gcttgcagca gatacaccaa tgccggattc
    17401 attcaaatct atgcctctgc caaaagacga accgagaaac gaaacattgc cggtagagga
    17461 agaagatctg cctgacccta aaaagaaagg acagaaaaaa tggtggctga tcctgttagc
    17521 tgtcctcgct gttttaggaa tcggaacagc tgtattcttt gcttctggcg gacgaggaga
    17581 agttgttgtt cctgatgttt ctgatctttc tgaagcttct gccagagaaa cactcgcaaa
    17641 agcgaaatta gaagtagcaa caaaaacaga agaaattgca gatgacaaaa tcgaagaagg
    17701 caatgtggtg aagacagacc ctgcagcggg aaccacagtc aagcaaaaac gcgaagtcac
    17761 tctttatatc agcagtggca cgaaaaaaat caaattagat gattacagtg gtaaaagtta
    17821 tgaagatgct gaagcaagtt tgaaagaatt aggattttct gctgatttga ttcaaaaaga
    17881 agaggaattc agttcagagg tagaaagcgg aatgatcatt tcgcaatctc catcagaaga
    17941 tacagaagtc gatcctaaaa cagacacgat tactttcaca gtcagcaaag gaccagaaac
    18001 agtgactttg agtagtcatg taggagaaaa ctacgaaact gtccgtaatt ggcttgttac
    18061 acaaggattc aatatcaata acattagtgc tacttatgac tatagcgaca ctgtgccatc
    18121 gggttcgatc attagtcagg atccagcctc aggtgaagtt gtagcagaag aaacttacat
    18181 cagttttgtc gtaagccaag gaacagaacc taaactgaat gacatctccg gctatacaaa
    18241 aagcgaagca caaagttatt tggctagtat tggtgcagaa tatatcggtc atgagacttc
    18301 tgaatattca agtacggtgg ataaggacaa agtaatccgt acagtgccag gggcaggtac
    18361 gacgattacc aaaggaatgg ttgtaaacgt gatttactct aaaggatcag acccagtgtc
    18421 ttctagttct gagaaagatt cttcagacag ttctagcagc tcaagtagct ctagcagtac
    18481 gagtagctct tccaaagaag aatcttccag caccacaagt tcatcagata gtacctcaga
    18541 aagctcaaag taagttttta ataaaataga acaaagaggt tgtgacagaa gtgatcagct
    18601 tcaagaaata agaaggaatt tacgaaaatt gattctcaaa tttttgtgaa atttcggttt
    18661 atttttcgaa gaagctactt ctggaacacc gtttactcgg aaaaagggac tgtgtcacag
    18721 ttcctttttt gtgttttcta tcccttttga ttagttttca agccttttac tttgagaagt
    18781 ttgcgtggaa cgacacctac tttccttttc atatggtaaa ataaacaaaa acggaggtgg
    18841 cacttatcaa aggacaaatc agaaaagcaa tcagcggttt ttactatgta tacgcagacg
    18901 gagagaccta tcaaacaaga ggaagaggga atttccggaa tcggaaaatc acgccgcttg
    18961 tcggagatca ggtaatcttt gagagtgaaa atcaaacaga cggctatctt ctggaaattt
    19021 tgccgagaaa aaatcaatta gttcgcccgc cagtcacaaa tgttgatctt ggaatcattg
    19081 tcacaagtat cgtggaacct gcattttctt ataatctgct tgaccgcttt ctagttacct
    19141 tggaatatga atcaatcgaa ccgctcattt ttttgacgaa gaccgacttg tcagacgacc
    19201 cgaaaaaaat cgaagaaatc aaacaaacct atactaagat aggctaccca gtgattgtac
    19261 ccaatcatgc gggtgacacg acagaattga ttcgttactt tccagaacgg ctatcggtct
    19321 tcatgggaca gtctggagca ggaaaatcaa cactactgaa tcaaatatct ccagaactga
    19381 atttagcaac aggagagatt tctgatgcac ttggaagagg gcggcataca acacggcatg
    19441 tggagctact ccctatctat gatggattag tagcagacac accaggattc agctcgattg
    19501 actttttgac tatcgaagca ggagaattgc caagacaatt tccggaattt gtggaagctt
    19561 ctgcccattg caaatttaga gagtgcatgc atgccaaaga acctggctgt gaagtgaaac
    19621 aaagagtaga aacaggtgat atcgcacaga cacgttacgt taattatttg caattcttac
    19681 aagaaatcga gaaaagaaaa cccatgtata cgaaaaaaga aggtaaacga aaataggagg
    19741 aaccaagaat gaaaatagca ccatccattt tgagcgcaga ttttgccaat ttacaaagag
    19801 atattgagtt agtagaaaaa ggaggcgcag attacatcca cgtagatgtc atggatggcc
    19861 aatttgtccc aaatatcacg ttagggccaa atattgttca ggcaatccga cctgtcacta
    19921 aattaccgct agacgttcat ctgatgatcg ttgatccgga aaattacatt ccagcatttg
    19981 cgaaagctgg agcagatatc attactgtcc atgtagaagc aactcctcat atccatcggg
    20041 cactccaaat gacaaaaagc ttaggtgtca aagctggagt agttattaat ccagggacac
    20101 cagtctccat gatcaaacat gttttgcctt tggctgatca agtgctagtc atgactgtca
    20161 atccggggtt tggcggacaa agctttattg aagaaacggt agaaaaaatc gcagaactcg
    20221 ctgatctacg taaagagaac aattggtctt atgcaatcga agtagatgga ggaatcgttc
    20281 cagaaacagc aaaagtctgt aaagatgcag gggcggatgt ctttgttgca ggatcatata
    20341 tctatggagc cgaaaatcca gtcggacaaa tcgaacggtt aaaagaggca ttagattaaa
    20401 tgaaaatttt attagctgct ggaggtaatc cggaaaactg gccgcagcct ctactgacca
    20461 aatacgatgc ctatgtcgga atagatcgtg gtgcgctgta tctgcttcaa gcaggctacc
    20521 cagtagatct ggcagtaggt gattttgatt cattgtcgaa cgaagagcag gaggaagtat
    20581 ttgcaaaagc caaagagtat gcccgttcac ccgcagaaaa agacgataca gatacacaat
    20641 tggggatctt aaagacattt gaacgttatc cgcaagctga agtcactttg atcggcgcca
    20701 caggcggaag attggatcat cttttagcca atctctggtt aggtctggaa cctcgcttca
    20761 aaccgtttgc ccatcagctt attattagag atcgtcaaaa ttatttggct tattatttgc
    20821 ctggaaagca tatagtaaga caggttccaa gtatgaagta tctagcttat tgctgtttga
    20881 cgcccgtctc ggatcttaca ttgaaaggca gtaagtatgt attggaaaat aaagcagttg
    20941 aatacccgac atcttatgca agtaacgagt ttctttcaga taccgctgaa ttttcttttt
    21001 cagatggtat gattgcagtt attcaaagca aagactgacc aatgaacaaa aggaaaaatt
    21061 ttgtctttca ggctaaaaat gacagaatct gttatttact aaaaaaaagc ttaagaggag
    21121 caacttgcct tttgtttccc ggcatttagt gataaaattg aacacaagag tctaattaat
    21181 tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat attattatta ccttggttat
    21241 tgttatcatc gtggtgacga tactaagtgt gaccgctgca cagcgagcga atgaaggaaa
    21301 gcctaatttc ttccaatcca tcgttaatga cagtgttgca atggttgata aaacaatttc
    21361 tgccccagta cggtgggtga gaaacggagt agattctgtc catagtcttt tttcaactta
    21421 tgccgaaaat gagcggctaa aagaaaaaat cgatagttat gatgacgttg cacagcaaaa
    21481 caagaattac gagagagaaa tcgaatcttt gaaaaaagaa cttgatttga accaaacgct
    21541 gacgagttac gaaaaagtaa cggcaaatgt cattactcgt tcaccagata gttggcagga
    21601 tatgctgatc gtagacaagg gttctaaaga cggtatcgaa gcaaatatgg cagtcatgtc
    21661 gcaaaaagga ttgattggcc gagtgatcga agtcaataca gcttcgtcta aaatcgaatt
    21721 actgtcatcc tctaacgaaa gctccaatca ttttccagta cgggtatctt cggctaatgg
    21781 cgaagcattc ggtttgctta aaaactatga tgaaaaactc catgcattag tggtgaccca
    21841 attgactggt gatacggata tcaaagaagg ggatgttgtt cagacatccg gtcttggagg
    21901 aaattctcca gctaacttgc cgatcggtac ggttatcaaa acgaaaccag acagttatgg
    21961 tctggatcgg gaagtgtatg tgaaacctta tgcagaaatg tatgatgtgt cagttgtgac
    22021 gattgtccaa cggctagcgg aggattagtc catgttgaaa aaagaaacaa tgaagtacta
    22081 tctgccaatc gttcttttct ttttgatgtt gctagatggc catttaacaa gaatgctagg
    22141 agagtggtcg aaaggcagct atatgtcaaa tgcccacttt ctgatcttgg cattgttatg
    22201 ttgtagtatg gcatttgaaa aacgctattt gctaatcacc acgattgttc tcggtgcagt
    22261 ctatgatgct tactatatcg gtgttatcgg tatctatgca gtagctctcc ctttaatcgt
    22321 atggttgatg tacgtaatga aagatgtcat ccacgtcaac atctttactg aatttttcag
    22381 tatgattatc tttgtcacgg gttatgaact gtttacgatg gtggtccaat tgatttttaa
    22441 actagcagta gtaaataaca cgtattttat tacaaggttc ttagggccta cactactgtt
    22501 gaacatgatt atttttgtat tattcatttt tccatttaag aaattattca gtgatgaata
    22561 atttcttttt ttgtttttcg tctgaaagta aaaaagaatt attgttatct taaaaaaagc
    22621 ccgtcttagc atgccttttt catgattatt ttctgaaacg aacagttttg tcatttttag
    22681 aggttgtttt gaaacatttt tgtaacataa ctattatatc gctgacatac tgctatggta
    22741 caatttttat atcgttaaga aagacttaaa tatatttaga tatttattaa attaagccag
    22801 tcggaggaat gaagagtgaa aaagagttta atatcagcag taatggtatg ttcaatgacc
    22861 ttgactgccg tagcatcacc gatcgctgcc gcggcggacg attttgattc tcagatacaa
    22921 caacaagatc aaaagattgc agacttgaaa aaccaacaag cagatgcaca atcacaaatc
    22981 gatgccctag aaagccaagt ttctgaaatc aacacacaag cacaagatct attagcaaaa
    23041 caagacacat tgcgtcaaga atctgcacaa ttagtaaaag atattgcaga tttacaagaa
    23101 cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac gtgaagcaca agtaagcaat
    23161 acaagctcaa actatattga tgctgtattg aatgctgatt cattagcaga tgctatcgga
    23221 cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg acttgatgga acaacaaaaa
    23281 caagataaaa aagctgtaga agacaaaaaa gctgaaaacg atgctaaact aaaagaatta
    23341 gctgaaaacc aagctgcttt ggaatctcaa aagggcgatc ttttatctaa acaagctgat
    23401 ttgaacgtac tgaaaacttc attagctgct gaacaagcaa ctgctgaaga caagaaagct
    23461 gacttgaacc gccaaaaagc agaagctgaa gcagaacaag cacgcattcg tgaacaacaa
    23521 cgtctagcag aacaagcacg tcaacaagca gcacaagaaa aagctgaaaa agaagcacgc
    23581 gaacaagcag aagctgaagc acaagctact caagcttcat caactgcaca aagctcagca
    23641 tcagaagaat catcagctgc tcaatcttca acgactgaag aatcatcatc agctgctcaa
    23701 tcttcaacaa cagaagaatc aacaacagcg cctgaatctt caacaacaga agaatcaaca
    23761 acagcgcctg aatcttcaac aacagaagaa tcaacaacag tgcctgaatc ttcaacaaca
    23821 gaagaatcaa caactgtacc agaatcttca acaacagaag agtcaacaac tgtacctgaa
    23881 acttcaacag aagaatcaac aacacctgcg cctacaacac catcaacaga tcaaagtgtt
    23941 gatcctggga acagtactgg tagcaatgca actaataaca caactaatac aacaccaaca
    24001 ccaacgcctt ctggaagtgt gaatggtgca gcaatcgtag cagaagctta caaatatatt
    24061 ggtactccat atgtttgggg cggtaaagat ccaagtggat ttgactgctc aggattcaca
    24121 cgctatgttt acttgcaagt aactggtcgt gacattggtg gttggacagt tcctcaagaa
    24181 tcagctggaa caaaaatctc agtttctcaa gcaaaagctg gagacttatt attctggggt
    24241 tcaccaggcg gaacttacca cgtagcgatt gctttaggcg gaggacaata tatccatgct
    24301 cctcaaccag gcgaaagcgt aaaagttggt tcagttcaat ggtttgcacc tgactttgct
    24361 gtcagcatgt aagaatgatt aatcaaaaag agttagtcct tttctggact aactcttttt
    24421 tattgagaga aaatagaaat cagatgaatt cttctgtcaa ttccatgaac gtttggatat
    24481 cttccatcac catatcgata cctgcttgcc aaaaatctgg ttgagtgaga tcaacattca
    24541 aatgtttctt tgcaagatct tccgtcgtca ttgaagcagt atcacgtagt aaatggatat
    24601 attcttcttc gaaatttgcc cctttttgat tagcgtaagc ataaatgcct aaactaaaca
    24661 ggtagccaaa ggtgtatggg aagttataaa acggtacgtc atcaataaag aaatgtaatt
    24721 ttgcagccca gaaatatgga tgataagtgg ctaaagctcc ttggtagcct tctttttgtg
    24781 cttcaaccat catctctgtg atcttatctt ctgagactaa tccttcttgc cgtgcttcat
    24841 agaatcgatt ctcaaaaatg aatcgagaat ggatattcat gaacatagct aaagcatttt
    24901 gcagttttgt atctagcaaa ttgatttttt cttcttttgt ttttgcggct tttaatgtag
    24961 catcggctac gatcagttct gcgaatgtac ttgctgtttc tgcgacattc attgcgtatt
    25021 cacgatttaa agaaggtaag tcccacatcg tgctgctatg gaaagcatga cccaattcat
    25081 gagcaagtgt cgcaacctca ttaacagaat tgctgtaggt cataaagatc ctcgattctt
    25141 ttgtttctgg taactcagta caataaccac caggacgttt tccaggacga tcttctgctt
    25201 caatccagct cttttcaaaa gcagattgtg cgaatttagc catttttgga ctgaatttat
    25261 taaagttttc gataataaat gcagctgctt cgtcaaaact aaaggttttt tctttcaaat
    25321 cgcctaaaat aataggtgca tcttgatcct gccattccat tttttcttta ccgaaaagat
    25381 tggcttttct agtcaagtaa tcaacaaggg gttgtttgtt tttttggatc gtctcccaca
    25441 tgacagataa tgtttctttt ttcaaacggt tgtattctaa tggtttttgc agaaaatccg
    25501 tgatgccatg gagttcatag ttagaaagtc tgaaaccatc taaatgattc aaagtatctg
    25561 tgaagagggg ggccttttct ttccatgctt tttcccatgc tgcgaaaagt gcttctctga
    25621 tttttggatc tcgatcgccc atcatcttat tgaaggcttg gccagcagat aattcgacta
    25681 cttgaccatc ttgttcgaag ggaatcgata tgctggctac aatcgtatca taatgactgc
    25741 tccaagcatt tagaccatct aatgagagcg tgttgataat gttctcttca gcttctgata
    25801 ataattgtga accgtcacga cggatctcgt ttaaacgaaa cgcaattgtt tcaaacgaac
    25861 cctgggaaag catctgattc caatcattgt cagatatttc tgcaaatttc ttcgacaata
    25921 ctgtttcggc tgattgtaga agaggaagta tggcgtaaag cttccctgat aagatctttg
    25981 catctgaatc attgacattg gcagaaagca atgcagtaat atagctgttg cattgggtaa
    26041 agccgttcgt aatcgtttct tgcaattgaa gaatcgcgtt tagctgctct gcattgtcag
    26101 aactaggtga ccattttgtt acacgttgat aatattcgtt cgtttggtct tccaattgct
    26161 tcatccgttg gttcaaagca tcagaatgac ttcctccggg gaaaatagaa tctagatccc
    26221 aatttaatga ataactcatg aaaaaactcc cttcatattg taatacaaaa ttaagtatag
    26281 cactttttta tagaagaagt ctctatgaaa atgattgttg tttcttcaaa aaaagtctaa
    26341 gataaataca gaggtgactt atgaattctt caaaaaaaca actgaaaaac ggaaatattt
    26401 atttagtgat cgcacttata gtcgttgcca tcttatttta cagttcttct caaacttacg
    26461 gacagcaatc acaagtttcg cgattagaaa gctggcttcc taatcagcca tttaaggaca
    26521 cattagcagg tatacgtttt aattatggtg gaagtatcgt gagtatcgac catctgggct
    26581 acgccaaatt tgtagagttc tttttaagaa aaggcgcaca ttttggtact tattttgttt
    26641 tgggtggcag cttgttttta ggtgttttcc ctaaactaaa aatttggtgg ctgacagcga
    26701 tccttgcttg gctttcagca acagggtacg caggtttgga cgaattccat cagatgttga
    26761 ccggtggacg aacacctatg ttccaagatg tcatgctgga ttcgatcggt gctctgactg
    26821 ctgtggtatt atgtgtgctt tttgcgacat tcaagaagcg tttatagatt ggctgtggct
    26881 ttgttttttt gtgaaatagt actagaatag aagtgtattc tcaaaagaac aagggaggaa
    26941 aaatcatgtc aggacatagt aagtggcata atatccaagg aagaaaaaat gcccaagatg
    27001 cgaaacgagg aaaagtcttt cagaaacttt ctcgtgaaat ctatatggca gctaaagcag
    27061 gcggtcctga tccatccatg aacccagctc tgcggcttgt tgtggacaaa gcaaaagctg
    27121 caaatatgcc aaacgacaat gtagaacggg cgattaaaaa ggctaccagt gccgtagatg
    27181 aaacaaatta cgatgaaatc acgtatgaag gatatggtcc gggtggagta ggaatcttag
    27241 tccatgcatt gacggataac cgtaatcgga cagcgacaaa tgtccgagta gcttttactc
    27301 gaaacggtgg gtcactggga gaaacgggtt cagtcagtta cttgtttgat cgtaaagggt
    27361 atatcgtcat tgaacgcgat gggttggctg ttgatgaaga gacgatgttt gaagatgtat
    27421 tagaggcggg ggcagaagat ctgcaaactt ctcctgaagt gttcgagatt tatactgcac
    27481 cagaagattt tgctgctgta cgagatgagc tggaaaaaga gttcacttta gcgcaagcag
    27541 aattgacgat gatcccacaa acgacgatcg atttaaatga ggaacaacgg gagcaattgg
    27601 aacgtctagt agataagcta gaagacgatg atgatgtctc agaagtcttt actgctgctg
    27661 aaatgtaagc aagtgcatag ttgagataat aagtaagaac gcacctgtac gattcgtaca
    27721 ggtgcgttct ttttatcaat tcttttttgt tagaagacca atacaggtgt tcctttttcg
    27781 accatcccga ataattcctt cattacactt ggcggagtgt tgatacagcc atgtgaaccg
    27841 cgggttttcc ataaatctcc gccgtattca ggttgccagt cagaatcgtg gataccaaca
    27901 cctgtccagt caatcggcat ccagtaattt actggactct cgtaaggtgt tccgtcgtca
    27961 tttgttcctt ttaaagttgc atcttcttct ttattccaaa catagaagac cccagcaggt
    28021 gtaggtgtag atggctttcc ggaaacgata tctgtttcaa gggctacttt tccgtcttta
    28081 taataccaca tatgctggtt ctctagatca acttcgatat acgtatcttc gatcaatgga
    28141 tgatcagcgg ttgtgccacc ttgcacaatc ggagaacgag taaagtcttg gcctgtcaga
    28201 atagcttttt tcagcgcttc tgtctcgcta tctgtttgga tcgtccagct gtaagtgcca
    28261 actgggactg taacttctcc tctttttgta cttttaaatt ttgtgtcatt cgtacttgtg
    28321 ttatactttg tgcctaaatc tgttacatat tgacgtactt gttctgtatc taaatcgact
    28381 tttccgtcgt aggtcagcca gctcatgata tcacttgatg ggatctggaa agtctccccg
    28441 tttatactgt aagtagcttt tacttttgcg atagcattca tggattcgag ctgttctttc
    28501 agtgaactgt ctccggaggt tacttttggt ttttctttga actcagttaa ttcaatcgta
    28561 tctttaccgg aatttactgc actttttact tgtttgaccg cagcatcgac gttgatggta
    28621 tttccattga cttcaggctt gataacaaaa gagtcgcctg atttttcaat tgttgcatca
    28681 gttgtaggtg tccggtcttt attcagatca gtcagtttag ttgtcaatgt ctgaacggta
    28741 gtatccagtt tctggtggtc ttgactgata ggatcgattt cttgtttttc agctgctgaa
    28801 acataagtca ttccccaagt ccatccgttt tgttcattta ggatcggaga aagctcagaa
    28861 gtgaaatcat ctttatagcc gagctcagat tttggtactt cctgccagac ttttccatct
    28921 tctttaatag ctaatttttt atcggaataa gcttctttta atttttcatt tgctttttcg
    28981 actgttagat tgctgacatt tattccgttg atttccgtat tcggcaaaaa atgctttgtg
    29041 tagtatgtac tgcgataggt ataaaagcta atcagcacca agagaacacc aagagctgaa
    29101 agcaaagcaa catgtttcga tttttttcga tgcgaagatc ttgtcatatc gacaactcct
    29161 taagtaaaag tttagggtgg aacggtctca tgatagcata atttagacga aaagacgaag
    29221 gttatctctt ctttatgaaa aaatttagaa accataagag tgataaaata aaaaaacgga
    29281 aaacaccgtt tagatgtttt ccgtcatatt caaagattat tcagcgtcta gtttagcaaa
    29341 agcttcatct agaatttctt ttaattgttt agcagaagca gccatacggt cttgttcgct
    29401 gtcgcttaaa ggaatttcga ttactttttg gataccttga cggttgataa cagcaggtgc
    29461 accaatgtag atgtcgttca atccatattc gccattcatg aagactgata aaggaagaac
    29521 agcattttca tcatctaaga tggcacgagt gatacgtgct aatgctactg cgataccata
    29581 gaaagtagca ccttttttct cgataattgt gtaagcagcg tcacgtacgc cgaagaatag
    29641 gttaaccata gcttcttcat cgatttctgg attgtttttc acccattcat agatttgcaa
    29701 gccggcaaca tttgcatgtg accaaactgg gaattctgaa tctccgtgtt cacctaagat
    29761 gtaagcatgg acgttacgcg catcaacgtc aactaattcc gcaagtgctt gacggaaacg
    29821 agcagagtca agagacgtac ctgaaccgat aacgcgttct ttagggaaac ctgagaattt
    29881 ccaagttgag taagtcaaaa tatctactgg gttagcggca actaaaaaga taccgttaaa
    29941 tcctgattca acgatttgtg tgacaacatc gcggttgatt tttaggtttt tgtttactaa
    30001 gtctaaacga gtttcacctg gtttttgtgg tgcgccagct gtaataacaa ctaagtcagc
    30061 atcatgtgcg tctgcgtaag tagcagagta gattttttta ggtgaagtaa atgccaaagc
    30121 gtgagacaag tcaattgcgt ctccttcagt tttagcagta ttgatatcca cgatacctac
    30181 ttcttgagca atattttgag taactagagc aaaggcatag ctagaaccta cggcgccgtc
    30241 tccaacaaga ataacttttt gatgatcttt tttttctgca gctgcagtca tttagtatca
    30301 atccttccat tgatttgttt tttcccatgc ttatgctaac atttctcaag ccccatgtca
    30361 cgctatttgg ctataaaata taacagataa caaggttgta tcatatgtaa aagcttacat
    30421 gtgtgttatt tcactatttt tataattcaa ttatttcatt aaactttgaa gaaattttta
    30481 atttctttat catttattaa cgattctctt ttagccgaaa cgctttgttt tgtgttatac
    30541 tatacattga cagtataaaa aagaaaaaaa gctcgatgga ctggaccgct aattagcagt
    30601 ccagcctttt gtgctgtttt tagaattagg tggaaaaaca aaaatgaaaa tgattgtcgg
    30661 gttaggaaac ccaggtacca aataccaata tactaaacac aatatcggct ttatggtcgt
    30721 ggatcaaatt gcacgcgaac atcaagctgc tttcaaaaaa aatccattcg aagcagaagt
    30781 tgccgaattt tttcataatg gagaaaagat ccttctagtc aaaccacaga catttatgaa
    30841 tgaatctggt cgtgcagtcg gaccgttaat gacttatttt gggatttatc cagaagaatt
    30901 agtcgtcatc tacgatgatt tggatctggc agtaggaaag atccggctcc gtcaaaaggg
    30961 gagtgccggc gggcataatg gtatcaaaag tatcatttcc catttgaata cgaatgtctt
    31021 tgatcgaatc aaggtgggaa tcggcagacc agaaggaaaa aaaacagtgg tccaacatgt
    31081 gcttagtcct ttcagcaaag aaaaccagcc tttgatagaa gaaagcatat ttcaatctgt
    31141 taaagcagtc gaatatctca tagaagggca ttcgttcgtg gatgcgatga atcaattcaa
    31201 ttgagaaagg aggaaaagga atggatatca ttcaattagt cggaaaagat ccgcaagtaa
    31261 aagaatggat cagcaattta gaagaaaaaa tgcctagaca actaatcaca ggattagctg
    31321 gctcagcgaa gacgcttcta tttgctcgag catttaagaa aacaaataaa aatatgatca
    31381 ttctgatgcc aaatttgtat tatgccaatc agttagcaga agatctgcaa catgtgattc
    31441 cgcaggatca gatccattta tttccagtgg atgaagtgct ttctgctgaa atggcctttt
    31501 cttctccaga agcgcgaggc gagagagtcg caacgctcaa cgcacttcaa gaaaaaaaag
    31561 caggtatcta tttgttgcca gtcgcagctt tgcacaagcg actgccgaat aagaaaactt
    31621 ggctacaggc gcaattgaat tggcatgttg gtgatgaaat agatgtggaa aatcttcctc
    31681 gccaattgac tttgatgggc tatgtacgag aagaaatggt cgcaaaaccc ggagaattca
    31741 gtattcgagg aagcatcgtc gatatttacc cattaacaac aaactatcct attcgtgtag
    31801 aattatttga tgtagagata gattctatgc ggtattttga agcagaaaca caacgttcaa
    31861 tcgaaaaaac ggaagaaatc tgggtttctc caacgagtga acaagtctat ggaccagagg
    31921 atctggaaca tggaatagag caaatgcaaa aaatgctaga aaaacggctt gctgcgacca
    31981 cagaagaagc agaccgcgag tttttacaag attacttcgg tcagatgact tcaagctggc
    32041 aagcaggtat cccgacagaa gaagcaaaat tctactcaga tcttctttac caagaatttg
    32101 taacgatctt ggattatttt ccttcaaata gtttactagt cgttgatgat taccagcgga
    32161 tcatggaaac aaatcgggaa atcgaaaggg aagcggcaga atggcatact caaaaaatca
    32221 gcgaattgcg ggttttttct gagcagtcat tttcagcaga tgtacaaaga atcgttcaaa
    32281 aggaaaaatt tactacaacc tttttctcac tcttccaaaa agggatggga aatcttcgtt
    32341 tccaagcaat ccataatttt caatatcgga cgatgcaaca gttcttcgga caaatgccat
    32401 tgctgaaaac agaaatggat cgttggcgca agcaaaaaca gactgttgtg atctttattc
    32461 cgacaaagga aagaatacgt aaagcagaag aaatgcttcg agatgaagat atattagtag
    32521 tagaaacaga aaaaaatgca ttgatcagag gacaggtaca attagttgaa ggagctttgc
    32581 aggccggttt tgaacttcct caagaaaaaa tagtagctat cacagaaaaa gaaattttcc
    32641 aaaaaacgac gaaaaaacaa acgagacgtc aaacagtaac aaacgcagag cgattgaaaa
    32701 gctacaatga gttaaaagca ggagattacg ttgtccatgc caatcatggt atcgggaaat
    32761 acattgggat ggaaacgcta gaagtagatg gcgttcacca agactatatg actattttgt
    32821 atcaaaacga cgacaaactg tttattcctg ttactcaact gaatctgatc caaaaatatg
    32881 tcgcctcgga agccaaagca cctcgcatca ataagctagg cggaagtgaa tggacgaaaa
    32941 ctaaacgaaa agtatcttcg aaaatcgaag atatcgctga cgatttaatc aaattatacg
    33001 cagcaagaga atcagaaaaa ggatatgctt ttggtcctga tgacgcctac caaaaagaat
    33061 tcgaaaatgc tttcccttat tctgaaacag acgaccagtt gagaagtgca gcggagatca
    33121 agcgggatat ggaaaaagaa aaaccaatgg atcgactact cgtaggtgat gttggttatg
    33181 ggaaaacaga agtagcgctt cgagcggcct ttaaagcgat aaaggaaagc aaacaagtag
    33241 cctttttagt tccaacaacg atcttggcac agcagcacta tgaaacgatg ctggaaagat
    33301 ttgaaggttt ccccgtaaat atcgggctat taagtcgttt cagaacaaga aagcaacaaa
    33361 aagaaaccat cgaacaactg cgcacaggtc aagttgatat tgttgtggga acccatcgaa
    33421 tcttgtcaaa agatattgaa tttagcgatt tgggtttatt gattattgac gaagaacaac
    33481 gttttggtgt aaaacataaa gaacgattga aacagttacg tgcgcaagtg gatgttttga
    33541 ctctgacagc aacaccgatt ccgagaactt tgcatatgtc gatgctaggc gtgcgggatc
    33601 tttcagtaat cgaaacaccg cctgcgaatc gttatccgat ccaaacatat gttatggaaa
    33661 aaaatcctgg ggctatccgc gaagcaatcc atcgagaaat gggacgaggt ggacaagtat
    33721 tctatttata taatcgagta gaaacgatcg aacaaaaagt agaagaaatc caagaactcg
    33781 ttccggaagc gcggatcggc tatgctcatg gacaaatgac agaggcgcaa ttagaaaata
    33841 cgctctttga atttattgaa ggtcagtatg atgttcttgt cactacgacc atcattgaaa
    33901 caggagtaga tatcccgaac gcaaatacat tgtttgttga gaacgctgat tatatgggtt
    33961 tatccacgtt atatcaatta cgcggacggg taggaagaag tagccgcgtc gcatatgctt
    34021 actttatgta tgagcagcag aagatcctta atgaagtcag tgaaaagcgt cttcaggcaa
    34081 tcaaagattt tacagaactg ggatctggat tcaaaattgc gatgcgtgat ctttccattc
    34141 gcggtgcagg taatctctta ggtgctcagc aacatggatt tattgatgca gtcggttttg
    34201 atatgtattc tcaaatgctg agcgaagcag tgtctcgtaa acaaggaaag aatagtcaag
    34261 tggagaagac cactgttgag atcgacctcg gtgttgacgc gtatctacct gaaacatacg
    34321 tggcagatca gcggcagaaa atcgagatct ataaacgaat tcgtgaactt gattcgcaag
    34381 aaatgctaga tgaactggaa gatgatctgc tcgaccgttt tggagaatac ccagaagaag
    34441 tagcacacct cctgacaatc ggacagatta aaatggatgg cgatcgagcg ctcttggaaa
    34501 cgataaggaa acaggagccg catatcgtct ttactctaag caaagttgga acgaaacgct
    34561 acagcgtgga acaattattc gaagcactaa cggcgacgaa actaaaagcc agtctaggcg
    34621 ttgaaaaaga acagatggta atacgcttga cgattccaaa ccaaatggaa gaagcagttt
    34681 ggcttcagga aatccaaaag tttgtcaaag cattgcgtga gcaaaaatat ctgtcagcgt
    34741 aggaaggagc aacgatttgg atcataaaga aatgcggcgc gtcatgcaag gagcatttgt
    34801 actgacgatt gcttctttca tagcaaaagt attaagtgcc ctttaccgaa ttcctctcca
    34861 aaacttagtc ggcgacgaag gtttttatgt ttatcagcaa gtttatccaa tttatgggat
    34921 agcaatgact ttagctttat caggtcttcc gcagttcata tcaaagtacg ttgcagaaag
    34981 aaagaatcct ttcgagcgaa agcaagcttt gcaaagtctt tatcccttgg tgttttggac
    35041 aggtgtcttt ctgtggttgt ttgtcttttc atttagcagt tggattgctt ggatgatggg
    35101 ggatatacaa ttgaaaccac tgatccaggt agtttctttt acctttttgc tgatgcctgg
    35161 tttatctttt tatcgaggga attttcaagg aagatttttg atggaaccaa cggcagtttc
    35221 tcaagttgtt gaacagctcg ttcgtgtttc tgtaatcgtc tgtagtgcgt ttgcttttag
    35281 aaagctcgga tggacggtgt accaaacggg gacggcagcg atgagtggag ccgtttttgg
    35341 cgggttatgc gcatatggga tccttcacta ctatgaacaa aagattcatg gaggagtatt
    35401 aagcttccgc cggttcccat tattcaaaca gccaccaaaa tctttgatcc ggcgctttct
    35461 agtagaaggc ggtctggttt cgatttatag cggattactc atcttttttc agctgatcga
    35521 ctctttcttt gtaaaaaatg ctttggaagt ttacggtcta tcggaatatg gtgcgaagat
    35581 agcaaaaggg gtctatgatc gagggcagcc tttggttcaa ttaggtttag tgatcgccac
    35641 cgcattaagt gcgacatttc tgcctgcatt gacccgtcat ttgaccaacc ggatctatcg
    35701 tcagttttta cagacagcca aaatctatct tagattgacg accgctttgg cattagctgc
    35761 atctttggga ttagccttac tgcttcctta tatcaattat gcgttgttta aagattatgc
    35821 aggaaatgca gcgcttgtat tgtttgtttt ttccattgct tttactgcag tcatccaagc
    35881 ctatcaaagt attgctcaaa gcaagaactc ttttcgccct tctttaaaag gagcaggatg
    35941 ggggctcttg gtaaaaggat tgactacttc ttttctaact ggtttattag gaacagccgg
    36001 agcaagcctg tctacccttt taggattagg tgtgacgctt tggtattttg tccgaatcga
    36061 aacaaaagag ctaaatgctt tttggaaaga acgacatttt gggcggaagt tgattatcag
    36121 ccttctttgt atgacagcag tcctgcttct gtattatggt gtgttgaata tagtgtttgg
    36181 tcctgtaact catcgttcca ttgctttttt tgtcagtgtg ataggcgttg ggattggcgc
    36241 atctgtgttc atttatgcag tgattcgtct ccgtctgttt acagtaagag agtggctcat
    36301 gttgccattt ggtaagaaaa ttctacgatt aaacaaaaaa atcaaaaagt gaggaataag
    36361 atatgcgatt agacaaattt ttaaaaatat cccgaatcat caaaagacga tcagtagcaa
    36421 aagaagtggc tgataaagga aggatccaaa tcaacggaaa attggcgaaa tcttccagtg
    36481 acgtcaaagt aggcgacatt ttgaaagtcc agttcggcaa taaaacgttg gaagtaaaaa
    36541 ttacggaatt acatgaatcg accaaaaaag aagatgcaca aaaaatgtac gaaattattt
    36601 ctgaaaatcg cacagaaaca ctagagaaat agattagaca agctttcatg atattggtat
    36661 aatgagaaag acaatcaaaa aagattggag cgaatccaag tgacgaaaaa aagcaataaa
    36721 atcgctgcgc tagatacgga atatgcaaag aaaaagtatg ctgaatttca gcaacaacaa
    36781 cgacaactga tttttcgaag acgcagactg gctgcaattt ttgttggtgc tttggtcatt
    36841 ttcgcgataa taggtgtgca gattttccat gatatgcaaa ggatgcatca attgaacgaa
    36901 ttgagagtcg aagcaaatac tgaaatgaaa gaagtcaacg cagacgttga tcagctgcac
    36961 caagatgttt cattgctgaa agatgatgaa tatgtagcta aacttgccag aagcaaattc
    37021 ttttactcaa aagacggtga aaaagtttat cctatattag aatcaaacac agacagctcg
    37081 acaaatgaaa cagatcaatc aactagttct acaactgcaa aataagcagg aatataaaaa
    37141 agggaaataa ttaggaggaa catttttttt atgtcaatcg aggtaggagc aaaactgcaa
    37201 ggaaaagtat caggaattac aaattttggt gcgtttattg atctaggtgc tggaaaaacg
    37261 ggtctagttc atattagcga agtgtcaaat ggatatgtaa aagacatcca tgatgtttta
    37321 agtgtaggag atgaagtaac agtcaaagtt atttcagtaa atgatgatgg gaaaatcgga
    37381 ctatctatcc gcaaagcgca ggaacaacct gtagaatctg gagaagaaag ccgtcctaaa
    37441 aaatttcaac aaaaagattc ccgtgacaac cgcaatcgtc cagcacagaa aaaatctttt
    37501 tctcgcagtc agccttcagc tcaaaaagaa gacttcgatt cattgatgag ttcatttttg
    37561 aaagatagcg atgaccgctt atcttcattg aagcgaaaca cagaaggaaa acgcggtggc
    37621 cgtggcggac gccgcagcta ataaagctga aaataatgat gatgcgacta ttttagtcaa
    37681 agtatcaaca gagggcaggt taattgtcgt tttagacgtt ttcccgctct ctttttttag
    37741 gaagaaaggg tgtggaaaga atgaacgttg aaaaagacgg taaaaaagta ccgctattgc
    37801 agcagtcatt tattcggcac ggaaatgaac aaggattctg gcaaaaagaa tcaaggattt
    37861 tagcagcggt atcaggtggg gttgattcga tggttttgct ccatttgctt ttgcaagcgc
    37921 aacgccaaat cggtttttct ttgggcgtcg tccatatcaa tcaccaattg agagaagaat
    37981 cagcagaaga agctgcttat cttcgctcat attgtgaaaa gaagaatcta cctttatatg
    38041 taagtgtttg ggaaaatccg gccaaaacgg ccatagaaac agcagctcgt aactttcgtt
    38101 atcaagcatt cactcgagtg atggaagaag gacaatatga tacgttgatg actgctcatc
    38161 atggagatga tcaaatcgag acggtgttga tgaaaatcat tcgtggagga cagttaagta
    38221 ctttttctgg aatcaaagga gtacaaccat ttgctacggg cagacttgtt cgtccattgc
    38281 tgagtttcag taaagaagaa ttatatgctt atgcagcaga atcacagctt gtttactttg
    38341 aggatcagac gaaccaatta ttagatgtcc aaagaaatcg gcttcgccat ttggttgttc
    38401 ctcaattaaa acaagaaaac acccaggtga tgagacattt tcaacagttt tcgcaacaga
    38461 ttcaatgggc agaccaagtc attcaaaaat gtatggggca gttaatcgaa aaggaagtcg
    38521 aacagttaaa ggatcgattt caattttcag cagaaatgat agaaaaaatg gaagaagcgg
    38581 aacgatacta ttttttccac tccttcttcg acaaagtatt ctcagaaaca ggggtcattt
    38641 tgaaagagca gcaaatgaca tcgattttag atcagtggca acagaaaaaa agtcaatggc
    38701 agcttagcat cggcaacaac tgggtactga aaagagagta tcattttgtt atactcgcta
    38761 aaaaagaaaa acaagccact gctcagacag aagatcagcg cttgttgctt attccagaac
    38821 aagggataca gttgaacgaa acagaatgga ttggtctggt caaacctgaa cattcagagg
    38881 acatcaaaac aacagattca tggtctctgt tttctcatga catttgggtt cccgccgatg
    38941 tgcaactata tgtcgaaaaa agaaaagcag gagaccgtat tcaattaacg gagaaactta
    39001 caaaaaaagt atctagatat tttatcgatc agaaagtacc taattcacaa aggcagcatg
    39061 cttggatcat tacagatgcc aaaagaaata tatatagcct gatacctttt gccttttcat
    39121 atttgagtat tcgtgaagaa actgataaaa tacactacat acttctttac aaatatcaaa
    39181 aagaagcaat tggaaggaga acctgatgtt ggaaaaggat attgagagaa ttttgatctc
    39241 acaagaagag atacaagttc gttgcaaaga actaggcaaa gagttaacag aaatttacca
    39301 aaacaccaat cccctagtag taggtgtctt aaaaggagcg gtaccattca tggcagatat
    39361 cgttcgttct atcgatacgt atttggaatt ggatttcatg gatgtttcaa gctacgggaa
    39421 tgcaacagtc tcgtcagggg aagtaaaaat cgtcaaagac ttggatacaa atgtagaagg
    39481 tcgcgatctg ttgatcgttg aagatatcat tgacagcgga agaacacttg cttatttagt
    39541 ggatcttttc aaataccgca aagcaaaatc agtcaaaatc gttacactgc ttgacaaacc
    39601 cgaaggaaga gtagtaaaca tcgaagcaga ttatgttggg ttcaacgtac caaatgaatt
    39661 tgttgtcggt tatggcttgg attatgcaga agcatatcgt aaccttccgt acatcggtgt
    39721 attgaaacct actgtttatc aatcaaatta atgctgtatc tcaattaaat tatggtacaa
    39781 tagaatggtc aggattgata aaaaactgtt aaattaaatt gacgcttagg gagggcaact
    39841 atgaacaaaa agaacaatgg catgatgaaa aatgccttgt attatgtcct cgtaatctta
    39901 gcgatggtaa tggtcgttta tttcattttt ggtaataacg gccagcgatc gtcagatatc
    39961 gagtactcaa aattcacaga acaactacaa gcaggcaaaa tcaaagagtt tgaagtgcag
    40021 cctgcaaatg gtgtatataa gatcactggt gaatacaagg aacagcagac agtcgctaac
    40081 agcggcgggt tagctatctt gggttcaaca gatacgacta ctacccattt cacaacaatc
    40141 attttgccaa atgatacaac attgagccaa gtaacagaac tggcaaaaga aaacaaagtt
    40201 gctacaacag taaaagaaga atcaagcagc ggcgtatggg tttcgttatt gcttagcttc
    40261 ttgccgatcg tgatcatcat ctttttcttc tacatgatga tgggacaaca aggcggcggt
    40321 ggtggcggtg gcggccgtgt catgaacttt ggtaaatcaa aagccaaaga agcagacaaa
    40381 aaagctaatc gcgtacgttt ttctgatgta gcgggtgcgg aagaagaaaa acaagaacta
    40441 gttgaagttg ttgaatttct gaaagatcct cgccgatttg ttgaattagg tgcccgtatc
    40501 ccggcaggtg tacttttaga aggacctcca ggaacaggta aaaccttgtt agcaaaagct
    40561 gttgccggag aagctggcgt accattttat tcgatctcag gttctgattt cgtagaaatg
    40621 tttgtcggcg tcggagcaag ccgtgttcgt gatttgtttg aaacagcgaa gaaaaacgca
    40681 ccggctatca ttttcatcga tgaaattgat gcagtaggtc gtcaacgtgg tgccggtatg
    40741 ggcggcggac atgatgaacg cgaacaaaca ttgaaccaat tgcttgtaga aatggatggt
    40801 ttcgacggaa atgaaggagt aatcgtcatt gctgcaacta accgttctga cgtactagat
    40861 ccagcgttac tacgtcccgg ccgttttgac cgtcaaattt tagttggccg tccagatgta
    40921 aaaggacgtg aagcaatctt gcgcgtccat gcacggaata aaccgatggc cgatgatgtt
    40981 gacttgaaag ttgttgctca acagactcca gggtttgccg gagcagattt ggaaaacgtc
    41041 ttgaatgaag cggcgctagt agctgctaga agaaataaaa agaaaatcga tgcttctgat
    41101 atcgatgaag cggaagatcg cgtgattgca ggtccagcta aaaaagaccg tgtaatcagc
    41161 aaaaaagaac gcgaaatggt tgcttatcac gaagctggtc atacgattgt cggattagtg
    41221 ttaagtcgcg ctcgggtcgt ccacaaagta acgattatcc ctcgcggacg cgccggtgga
    41281 tatatgatcg ctttaccaaa agaagatcag ttcttgatga caaaagaaga tatgtttgaa
    41341 caaatcgtag gcttgcttgg tggacgtaca gctgaagaaa tcatcttcaa tgtccaatca
    41401 actggggctt ccaatgactt tgaacaagcg acagctttag cacgaagcat ggtgacagaa
    41461 tatggaatga gcgatcgttt aggtcccgtt caatatgaag gaaatcatca agtgttcgtt
    41521 ggccgcgact acggtcaaac aaaagcttac tctgaacaag ttgctttcga aattgatcaa
    41581 gaagtacgca agattttgat ggaagctcat gccaaagctc atgaaatcat cgaagctcat
    41641 cgtgcacagc ataaattgat tgctgaaaaa cttctggaat tcgaaacatt ggatgccaaa
    41701 gcaatcaaat cgttattcga acacggtgtg atgcctgaag gtactgagtc aaatgattat
    41761 ccaagcgaaa aagaagcgaa aacttttgaa gaagcaaaac gtgctttgga agaaaaagac
    41821 gctgaaaaac aagcagaaga aaaacgtgat tttgaagaag caaaacatga gctgcatgag
    41881 gaaaataaag cagaacatga ttcagattct caacgatcag aagataatca ttcagacgat
    41941 caatacaaat aaaaaaaata taggtcaatt gatctgatag gactcccgaa aaccggaaca
    42001 aaaaccggtt ttcgggagat ttctttattt aagatgtaaa taggatgatg tcctgttatc
    42061 tagatgattc tgaattattt ttaaaagaag cattccaaag ttgaaaaaag caggagttgc
    42121 tagtatgatt cactatgagt ttaaaaacgg aaaaaggagt acataacaaa tgaaagatta
    42181 tctggttaag gcacttgctc acgacggatt cgtacgagca tacgccgttc aagcaacaaa
    42241 taccgtagcg gaggcacaaa gacgtcatga tacttggcat acgtcctcag ctgctttagg
    42301 acgaacatta gtgggcagtc tgctgcttgg tgcaacactt aaaggagaag ataaactaac
    42361 tgttcgaatc caaggcgatg gtccagcagc aggaatcgta gtagatgcaa atgggaaggg
    42421 agatgtgaaa ggctacatca aaaatccgca tctcagcctt cctttgaata gtgaaggtaa
    42481 aatcgatgtg cgaggagcag ttgggacaca agggatgttt actgtcacaa aagatttagg
    42541 attaaaagag cctttttctg gacaaacgcc aattatttct ggagaaatcg ctgaagattt
    42601 tacttactat ctcgctatct cagaacaaat tccttcagct gttggactaa gtgtactggt
    42661 caatactgat gacagtatca agacagcagg tggattcatg attcaaatca tgccaggtgc
    42721 cgatgaagaa acaatcagca agattgagga acaattaaaa acaacaccaa gaatctctac
    42781 tctcttagat gaaggacaga caccagaaga aatattgaag ggattgcttg atacagaaga
    42841 gctgaacgtt ttagaaaaaa tgcctgttca attcaaatgt gactgttcga aagaaaaatt
    42901 tgcagaagca attattgcgc taggtgcaga agaaatccaa gcaatgatag atgaagatca
    42961 tggagcagaa gcggtatgct ctttctgtaa caataaatac aagtatagtg aagaagaatt
    43021 agctggattg aagaaagaag ccttaggagg aatttaagtg gaacacaaat ggaaaatcgg
    43081 caatgttgaa atccctaatc gtgtcgttgt tgcgccgatg gctggaataa caaattcagc
    43141 atttcgagtc actgtcaaag aatttggtgc aggcttagtc gtatgtgaaa tgatcagtga
    43201 ccaaggtatc catttcagaa acaaaaaaac tttagagatg ctccacattg aagaaacaga
    43261 acatccatta agtttacaga tatttggcgg gaacaaagac agcctagttg aagctgctca
    43321 atttgtcgaa gaaaacacag ctgcagatat aatagacatc aatatgggct gtccagtaaa
    43381 caaagtgatc aaagcagaag caggggcaaa gtggctttta gatcctgaga aagtatacga
    43441 aatggtccaa gctgtttctt cagcagtgga tatccctgta acggtcaaga tgcgtatcgg
    43501 ttgggatgaa gaacacgtct ttgctgtaga aaatgcatta gcagcacaat cagctggagc
    43561 aagtgccatc gctatgcacg ggcgtacacg ggttcagatg tatgagggaa aagcagattg
    43621 ggaaatatta aaagaagtaa aaaaacatct caccatccca tttatgggga acggggatgt
    43681 caaaacaccc gaagatgcaa aacggatgct tgattatgtt ggtgcagacg gtgtgatgat
    43741 tggccgggca gctttgggaa acccatggat gattcatcgt acacagcatt atctagaaac
    43801 aggagaactg attcctgagc caacaccagc cgaaaagatt gctacagcta aacttcattt
    43861 acaacgctta gcagatctta aaggagaaaa agtagcatgt agagaattca gaacacatgc
    43921 ggcttattat ttaaaaggga ttcctcgagc agcaaaagtg aaagtagcaa tcaatcagac
    43981 agaaaatcag caagaaatca atggtctgct ggatcaattt gtagaaaaag cagaaaaagt
    44041 actattgaaa caaacgttgt gatttctaag tttttcgtga attttcaagc tgaaattcca
    44101 agttcggctt gctttttttg tgtttcattg gcattatagg aaagagatta acatgagaat
    44161 ggaggaactt ttgtgacaca ggaacaacaa catcaagtag aagatttaaa tgatcaaatg
    44221 cttgtacgtc gtcaaaaaat ggaacaattg cgtgaagatg gaatcgatcc atttggaaaa
    44281 agatttgaac gcacacataa ctcagaagaa ttgcatgaac tatttgatcc aagaacaaaa
    44341 gaagaactag cagagatggg attgaccgct agtgtagctg gtcgtatgat gactcgccga
    44401 ggaaaaggaa aagctggatt tgcgcatcta caagatcgag aaggccaaat tcaaatttat
    44461 gtaagaaaag accaagtagg tgatgaagcc tatgaagtat ttaaacatgc agaccttgga
    44521 gatttctttg gtgtaactgg acaaatcatg aaaacagaca caggcgaagt ttctatcaaa
    44581 gcttcagaaa tcacgatttt gtcaaaagca cttcgtccac tgccagataa ataccatggc
    44641 ttgacgaata ttgaacaacg ctacagacaa cgctacttgg atttgatcag taatcgcgaa
    44701 agctttgacc gatttatgaa acgaagccaa attatcagcg agatccgtcg ttatttagat
    44761 ggcaatggct atattgaagt agaaacgcca gtattgcata atgaggcagg tggagccgct
    44821 gctcgtccat tcatcacaca ccataatgca ctggatattg atttatattt gcgtattgcg
    44881 ttggaacttc atttgaaacg actaattgtc ggcggaatgg aaaaagttta tgaaatcggt
    44941 cgtgttttcc gtaatgaagg gattgacact acgcataatc cagaatttac gatgttagaa
    45001 gcatacacag catatactga tttcaatgat gtaatgaatc tgacagaagg tattatccgt
    45061 aacgcagctg aaaaagtact agggacagcg aaaatcacat atgatggaca agctgttgac
    45121 ttagaaagcg actttaaacg tatccatatg gtagatgcga tcaaagaaca aactggtgta
    45181 gatttctgga aagaaatgac attggaagaa gcattagcct tagctgaaaa gcataacgta
    45241 gaagtaacag aagcgatggg agtcggacat gttatcaatg aattctttga aacgtttgtt
    45301 gaagaaacgt tgacacaacc gacatttgtt tacggacatc cagtcgaagt ttcgccttta
    45361 gcgaagaaaa atccagaaga tcctcgattc acagaccgat tcgaactgtt tatcgtagga
    45421 cgcgaatttg ctaatgcctt tacagaacta aacgatccaa ttgatcaaag agaacgattt
    45481 gaagcacaag aaaaagaacg tgaactaggg aacgatgaag ctcacggtgt agacgaagac
    45541 ttcttggaag cattagaata cggtatgccg ccaacaggtg gtctaggtat cgggattgac
    45601 agattagtga tgctgctaac tgacgctcaa tccattcggg acgttctact attcccaaca
    45661 atgagataat caaataaaag ctgtctaggg gactccttag gcagcttttg ctatgtagaa
    45721 agcaacaaac aataacgaat gttcaaaagc tgttatcaaa tattgcagac attaatggag
    45781 ccgaaagcga acgataaaga aagaaaagta atgaataatc gcttattgaa aaaaacttga
    45841 aaaaatacaa aaaattcgtt gaccaagata aatttagttg gtatattatt acttgtccaa
    45901 aagacagaag gaaatatttc ttcaaaaaca taacaaaaaa gttgttgaca tgttgctaac
    45961 ggcatgataa gataactgag tcgttaaaaa agaacagctt gcgaagcttg attctatcga
    46021 aaaaaacttc aaaaaagttg ttgacaaata acaaacgatg agttatgata aatgagttgc
    46081 tgaaagattt caaaaaacga aatcgaaaaa aacttttaaa aaagagttga caatcacttg
    46141 ccgctctggt atgatataaa agttgctacg gcagccaaaa caaatcggaa aaacatttcc
    46201 gatgaagtag acctttgaaa actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt
    46261 gattcaattc aagacaacaa acatttttaa caagcaagca atatgctagc aaacaaattg
    46321 agcttaacaa tcg
//
LOCUS       DADJYG010000008        44698 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.008, whole genome shotgun sequence.
ACCESSION   DADJYG010000008 DADJYG010000000
VERSION     DADJYG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.008"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            210..434
                     /gene="nrdH"
                     /locus_tag="JZ382_000363"
     CDS             210..434
                     /gene="nrdH"
                     /locus_tag="JZ382_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY2426576.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            593..2752
                     /gene="nrdE"
                     /locus_tag="JZ382_000364"
     CDS             593..2752
                     /gene="nrdE"
                     /locus_tag="JZ382_000364"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY2426577.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALAAFFEENVK
                     PNTMTFPSIMEKINYLIEQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVDITAEYDRLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNTLKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2802..3767
                     /gene="nrdF"
                     /locus_tag="JZ382_000365"
     CDS             2802..3767
                     /gene="nrdF"
                     /locus_tag="JZ382_000365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY2426578.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3814..6237)
                     /locus_tag="JZ382_000366"
     CDS             complement(3814..6237)
                     /locus_tag="JZ382_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HAY2426579.1"
                     /translation="MEKEKKGLGRRILLMSMLSLISLFGMSISTFAEEDEFQKMEERW
                     KSQLVFEKESPENESLRAYIEALSKEAENLYQTLDQSPDRTYLWPLESGNTSSADLTT
                     QFTKLQKLTYAYKTKGTTLYQQPEVAAAIKEGIDFMITKKGYDGKKYYGNWWDWQIGI
                     PQKFVNILLLLHQELSSEKIQQYTAILNQYVPDPFQQLYTKPQDSFVDLAFIPNFSTT
                     GANRTDLSLTVLGVGILQKDATKIHQAVNGIKDVFQLVTSGDGFYADGSFIQHNDIPY
                     TGSYGNVLIKGVGQIFTIVDGTSYAAASDDIHAFVENVEKAFIPLIYQGEMLPMVNGR
                     AISRAPSKTKVGYGSSTMYNLLIVSKFAPEDKQKELKEAVKYWMQLNPSYYITNVRDY
                     NDLVMTLSLLEDQTILGKDLPFIGTKMYHKMDRFVQRTSKYMVGISMYSNRISSFEAG
                     NKENKRGWHTSDGMVYLYNEDDVQFGKSYWPTVDPYRLPGTTVDTVPLKDEISSFTTV
                     RSNEKAVGGVSNEGQAVVGMALNKTGTKNNGTVLPMDVKGKKSWFVLDDQLIALGSGI
                     TGNTNASIETIIDNRLLNDQFTYKVVTETGEITQPTEQSEKEWLLLQSSQPETSIGYY
                     FPEKETIKVISEARQGNYREINESFPSDEVYHGSYRKFLINHGKHPINEAYAYVILPG
                     VNEKGLKEYAEKEPVTILQNTPKIQAVKVKESGYLGINFWDNTGGELDGLKTDKPLSV
                     LKKINEQEKSYTFADLAHTKTKITIELPNDFEAIQSMSEGITYDEQMGRFTIDFSQTS
                     DTQKQIVVE"
     gene            complement(6373..7182)
                     /locus_tag="JZ382_000367"
     CDS             complement(6373..7182)
                     /locus_tag="JZ382_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2426580.1"
                     /translation="MMGMNNQKLTKQELNAISWRYILGSQLNWNYERMMSSGYLYGIL
                     PVLKKFYGNDESHLQDMMRTHNQFFNTNAIFGNLIMGIDVAIEEEDGYKAKDTIIALK
                     TALMGSLAGVGDSLFHVIWGTIFGSIAGTLAQSGSIVGCVIWVIANIVLLFGRAALLP
                     LGYKQGVKLVTTLKNKLSAFTNAATVLGVTVIGALIPSVVKATVPFVYKKDGVELVIQ
                     DTLDAILPSLVPVLLVLLTYWMLGQKKLNSTRVIWIILILSIALSAFGILA"
     gene            complement(7166..7963)
                     /locus_tag="JZ382_000368"
     CDS             complement(7166..7963)
                     /locus_tag="JZ382_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426581.1"
                     /translation="MHLAAYQIILITVYAFIAINDSLISNTLTQPAITGMISGMIMGD
                     LKTGLMVGGTLQLMRLGIAAFGGASVPDYFTGAVLGTVFAVISGKGAEYGIGLAVPVS
                     LLMLQLDVVARFCNVYLLHRVDRAIDDMKINRIPRLVLSGSFLWGLSRAIPILLMLLV
                     GDAAVSTITENTPEWLMNGLKTAGGVLPVVGVGILLHYLPTKQYIPYLLLGFFLAAYL
                     QVPMLGVSIVGVIAAIIVFKKDSEKAEHPTVTATNNGTFEGGYDGDE"
     gene            complement(7987..8913)
                     /locus_tag="JZ382_000369"
     CDS             complement(7987..8913)
                     /locus_tag="JZ382_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAY2426582.1"
                     /translation="MQNIILISHGSMAEGVKASLEMIVGKQDHVHVVALTPDGDNRQF
                     EEELLKKMKALNGSSLIIADLLGGTPCNVALSNYLESENVEIIAGMSLPLVIEATLNS
                     TATVKELIQAATTGIVDVKQKMKQTEIEKEPSLPKDNSQLYEAYKGKENIVNVRIDER
                     LIHGQVAGIWAPSLHTQRIIVINDEAAADSLQKSSLRMAAPTSMRLSVLPVESAAKNI
                     RSGKYGKQRLFLVFKNPTDVLRYLKAGGKLTHVNVGNMSYKEGSKDITKSIKVLEEEI
                     DVFESIAAMDINVTAQLVPNDPVVDFMDKLRN"
     gene            complement(9125..9814)
                     /locus_tag="JZ382_000370"
     CDS             complement(9125..9814)
                     /locus_tag="JZ382_000370"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY2426583.1"
                     /translation="MSKVLFSPSLMTMDLDKFKEQIEFLNHHVDSYHIDIMDGHYVPN
                     LTLSPWFIEEVRKISHLPMSAHLMVTDQSFWVNQLISQKCEWICMHVECLDGLAFRLI
                     DQIHAAGLKAGVVLNPETPIETIFPYIDLVDKITIMTVDPGFAGQRFIESTLDKIVDL
                     RKLRQEKGYHYLIEMDGSSNRKSFKRIDHAGPDIYIIGRSGLFGLDEDIEKSWAIMKR
                     DYEETTGKSIE"
     gene            complement(9871..10968)
                     /locus_tag="JZ382_000371"
     CDS             complement(9871..10968)
                     /locus_tag="JZ382_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY2426584.1"
                     /translation="MEKIKKLNLKGHLLTAISYLIPIVCGAGFLIAIGMAFGGTSQGT
                     LVQGEFSFFDALATMGGAGLGLLPVVIATGISYSIAAKPGIAPGFIIGLTANAIGAGF
                     IGGILGGYLSGFLVLAILNHVKVPNWAKGLMPTLIIPFVASILGGLIMVYIIGTPVAL
                     FTSILTNFLEGLGNSSLLIFGGVIGLLSGVDYGGPINKTVFAFVLTMQAEGINGPITA
                     LQLVNTATPIGFGLAYFVAKIFRKNIYTKVEVETLKSAVPMGVINIVEGVIPLVMNDI
                     VRGVVATAIGGFAGGATTMILGADATVPFGGVLMIPTMSRPWAGVIAILVNVLVTAVV
                     LAIIKKDVGKEVESVVEKEEEDIDLGDIQIF"
     gene            complement(10988..11308)
                     /locus_tag="JZ382_000372"
     CDS             complement(10988..11308)
                     /locus_tag="JZ382_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAY2426585.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKKAGHEMHIETQGTIGI
                     ENELTPDQIKEADLVILAIDVKISGRERFEGKRIIQVPTEIAVKSPNKLIEKAQEIIE
                     KQLA"
     gene            complement(11322..11717)
                     /locus_tag="JZ382_000373"
     CDS             complement(11322..11717)
                     /locus_tag="JZ382_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY2426586.1"
                     /translation="MKDEFIEKIEVSVTSQREAYHWLASSLYPGEEEKISILMEAFLM
                     REQNGNIQIEEGIILPHIEHELITESKIIILYPQKTIIQWNEAIEQVDLILFVLLSPN
                     ETTEKKKKIANFMRCLADERFIQTLKKRK"
     gene            complement(11707..13187)
                     /locus_tag="JZ382_000374"
                     /pseudo
     CDS             complement(11707..13187)
                     /locus_tag="JZ382_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            13465..14172
                     /locus_tag="JZ382_000375"
     CDS             13465..14172
                     /locus_tag="JZ382_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2426587.1"
                     /translation="MIPKYEQIKQDLLEEIKNHHFIPGDRFYSEADIKRKYSVSSITA
                     VKALNELTAAGYLYRIQGKGTFVSKSKVSQSVKFSDIELHSLDKEKVKVLSIEEENEM
                     DILKELGLPANASYYKIKRVRFFEDTPFLVHITHLPKKLVKEPIAKDISVYSSIYEKV
                     RKDFGIDLFSLASVETNEIVFPEHAELLNLLNLSFREPVVQQIKHSYLADQSVAEYII
                     SYKHWKYFKTKIEVEAE"
     gene            14299..15462
                     /locus_tag="JZ382_000376"
     CDS             14299..15462
                     /locus_tag="JZ382_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAY2426588.1"
                     /translation="MNLQQKKEMLIELGTFVDKNWLDEQFEWCMMRIKKNMVRFGLDF
                     PSACATNGKYRIKKNDDWTNGFWTGMLWLSYEWTKEEKFLVRAMENIHSFQKRLDQHF
                     ILDHHDIGFLYSLSAGAGYKVVDSQLCKESLLQAANILVARFQNKGNFIQAWGAYGDP
                     LEYRLIIDSLINLPLLFQACTLSNDPHYKEVAFKHYQTLLHTVIREDATTFHTYYFDS
                     NTGVPTHGATHQGHSDESIWARGQSWAILGIPLNERYLHSAPFPLNYKAIVDVFLEHL
                     PQDLVPYWDFDFNDDFPSDKDSSSLAIAACGLLEAEKMEAYPEAKEIAKGMIYQLGTH
                     YTAKNDPENEGILLHGVYAHAEGKGVDEPNLWGDYFYLEALMRLAKPEWNTYW"
     gene            15478..17265
                     /locus_tag="JZ382_000377"
     CDS             15478..17265
                     /locus_tag="JZ382_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAY2426589.1"
                     /translation="MQTFKIKDEFLVDGIPTKIISGAIHYFRIPPSQWEHSLYNLKAL
                     GANTVETYIPWNLHEPQEGSFDFSGFKNVVRFVKIAQELDLMVILRPCAYICAEWEFG
                     GLPAWLLKEPNIRVRSTDPRFMEKLKNYYQVLLPKLAPLQITQGGPVIMMQLENEYGS
                     YGMEKSYLRQTKELMLAHSIDVPLFTSDGAWLEVLDAGTLIDEDIFVTGNFGSHSKEN
                     AQVLKEFMQNHQKNWPIMCMEYWDGWFNRWGEPIITRDPEELATEVKEMLEIGSLNLY
                     MFHGGTNFGFYNGCSARGNTDLPQITSYDYDALLNEAGQPTEKYYAVQRVIKEVCPSV
                     WQAEPRTKTLKNLGTYPVNRSVSLFHIKEQICEEIKTDYPLTMEQASNGYGYLLYSLT
                     LKNYGHKNKLRLIETNDRAQIYIDGKYDQTQTQETLGDEMMIEGQKNQPTIALDVLVE
                     NLGRVNYGAKLNSPSQSKGIRNGVMQDIHFHLGYRHYPLTFEQAQLDKIDYSAGKDPS
                     QPSFYQFEFDLAEEADAYIDCSLYGKGIVIVNGFNLGRYWNHGPVLSLYCPKDVLKKG
                     RNEVVIFETEGISIDTLIFSDQPIKKDLS"
     gene            complement(17392..18225)
                     /locus_tag="JZ382_000378"
     CDS             complement(17392..18225)
                     /locus_tag="JZ382_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426590.1"
                     /translation="MIQIDLKNKNNQVVIGKIDHEKKETVFSANGEEIAVLAIKNHTY
                     EVGDKLVITKEKNDYYFVVQLDETLAPTLLYIPEKKWEYVIPLEETKASVETGFRSKR
                     HHIYVRKAYDFEVESYRNVSTNPHDQKLFSGAYPHAYANVETRNDVTFFAKNAIDGKF
                     GNRSHGSYPFTSWGINQQKDATLTIDFGRSIQTDRVNLLFRGDYPHDSYWTEVSICFS
                     DGETIKVATTDTLEFQSFYFPEKMTSSVTLKNLKKAEDTSPFPALTQIEIFGREVITC
                     T"
     gene            complement(18212..19948)
                     /locus_tag="JZ382_000379"
     CDS             complement(18212..19948)
                     /locus_tag="JZ382_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAY2426591.1"
                     /translation="MNQLKQNLFLTRRDFEEALEEIILPLKQELFNSEKTGVRLGSSG
                     AVYDEKRAEMEAFIRPLWGLAPYWMNHETKDELKDVYRKKILKGMNPKDSFYWGDIND
                     YDQYIVEMAALSLNLLLNRNYFFDELSGTSKQQLIHWLKQALDKKIPKNNWTFFKILI
                     RIALKHCGESLDKEKMEAELRFIDTMYVGKGWYIDGKSSQKDYYVPFAFHYYGLIFAK
                     LMKDDFPEWSEKFIYRAKEFTKDYIYYFDSDGEALPYGRSLIYRFAQGAFFSALIFAE
                     VEAIPWGQIKTLLSNHMKQWMSHDIFTYDGRLSIGYHYENLVMAEGYNAPGSPYWALK
                     VFLLLATKKEHPYWEAKPEPVNTKEKRLIDNGNMLLCQAKEGKHVLGYPHGLMIDGQA
                     HALAKYSKFVYSTKFGFSVPKAGITYEEGAFDNTLALSRDGEYFRTKKKVRSSEATET
                     FVMYEWQPFQEVLIQTEIYPFGEWHVRFHEIYTTIPLEIREGGFSLLVKEPEITLSKD
                     CAKIKETHVSQIIAIEGYEEAGIQVLEPNTSVFFPRTNLPYLKKKIEPGTHRFICLVG
                     GIINGGEKNDPN"
     gene            complement(20329..21468)
                     /locus_tag="JZ382_000380"
     CDS             complement(20329..21468)
                     /locus_tag="JZ382_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY2426592.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIVGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVLGFLLTFLVVIILVVLAVMNVVPQLVEQT
                     IQLTQSLPIYADETSKWLNELVQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     TKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFNQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRKMEHVSEKLDSPKVLEETSE"
     gene            complement(21647..22360)
                     /locus_tag="JZ382_000381"
     CDS             complement(21647..22360)
                     /locus_tag="JZ382_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAY2426593.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALAYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(22619..23320)
                     /gene="nagB"
                     /locus_tag="JZ382_000382"
     CDS             complement(22619..23320)
                     /gene="nagB"
                     /locus_tag="JZ382_000382"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY2426594.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            23554..25086
                     /locus_tag="JZ382_000383"
     CDS             23554..25086
                     /locus_tag="JZ382_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAY2426595.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLSIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIRKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(25142..26332)
                     /locus_tag="JZ382_000384"
     CDS             complement(25142..26332)
                     /locus_tag="JZ382_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2426596.1"
                     /translation="MESKEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAISLLAAFFAPSFAS
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTSVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTISGLVFQGCAFFFQFVVFRE
                     KKEN"
     gene            complement(26414..27103)
                     /locus_tag="JZ382_000385"
     CDS             complement(26414..27103)
                     /locus_tag="JZ382_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY2426597.1"
                     /translation="MKKKKIRLILITLGIVLILMVGYRLYLEYQTDIQLFLNPRASKE
                     ELLAHIRSHGFSASLLLIVLTAVMCAVPGIPTSVIGVIAGLSFGPFIGAGINVLGNAL
                     GNISAVFLMHRLKFLDKKTEKNHWVQSIRQMRHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIVMSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQRQK"
     gene            complement(27210..27578)
                     /gene="rplL"
                     /locus_tag="JZ382_000386"
     CDS             complement(27210..27578)
                     /gene="rplL"
                     /locus_tag="JZ382_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY2426598.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEADELKAKLEEVGASVTVK"
     gene            complement(27631..28134)
                     /locus_tag="JZ382_000387"
     CDS             complement(27631..28134)
                     /locus_tag="JZ382_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY2426599.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(28544..29233)
                     /gene="rplA"
                     /locus_tag="JZ382_000388"
     CDS             complement(28544..29233)
                     /gene="rplA"
                     /locus_tag="JZ382_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY2426600.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFATIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(29337..29759)
                     /gene="rplK"
                     /locus_tag="JZ382_000389"
     CDS             complement(29337..29759)
                     /gene="rplK"
                     /locus_tag="JZ382_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY2426601.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            30102..30770
                     /gene="sdaAB"
                     /locus_tag="JZ382_000390"
     CDS             30102..30770
                     /gene="sdaAB"
                     /locus_tag="JZ382_000390"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAY2426602.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTGILSETNTNIGTMTVTRESKGESAIMIIEVDNPQVEETVRRLKHLPNIDSV
                     NYFD"
     gene            30783..31655
                     /gene="sdaAA"
                     /locus_tag="JZ382_000391"
     CDS             30783..31655
                     /gene="sdaAA"
                     /locus_tag="JZ382_000391"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAY2426603.1"
                     /translation="MFLSIEELVDQAKAYSSVAELMIAVEMEMSGRSRSQIIAIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLGLSHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLTEEILNKRS"
     gene            complement(31749..32849)
                     /locus_tag="JZ382_000392"
     CDS             complement(31749..32849)
                     /locus_tag="JZ382_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAY2426604.1"
                     /translation="MTYHTSNEEHPIDIVNIASLEGRVKERMEAGAFGYIRGGSEDEW
                     TMKENTTSFNAKKIMPRILRGIDSADLHTSVFGIELDTPIIQAPSAAQGLAHEKGEAD
                     TAKGVAAAGSIFSISTYANTTIKDAADAAPGAPQFFQLYMSKDDGFNEFILNKAVEAG
                     AKAIILTADSTLGGYREEDVINQFQFPLPMPNLAAYSEQSASGNGEGKGIAEIYAAAK
                     QGLTPDDIKTIKEITNLPVIVKGIQSPEDAVIAISAGADGIWVSNHGGRQLDGGPASF
                     EVLPKIAEVVNKRVPVIFDSGVRRGEHVFKALASGADLVAIGRPVIYGLNLGGAEGVT
                     SVFEHLNKELSITMQLAGTKSIDEVKNTKLMD"
     gene            complement(32941..33822)
                     /locus_tag="JZ382_000393"
     CDS             complement(32941..33822)
                     /locus_tag="JZ382_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY2426605.1"
                     /translation="MTNRIKNVCKHAATAALVLISLFAISGCQTTKTTAVSDKEKTVE
                     SSKVIEKTTIPTLFFHGYSGTENSFKGMLQRLEKNQKAVKELVLIVEADGQIQAEGDL
                     SGKADNPMVQVLFADNKNNEWNQAEWIKSCLLYLKENYSIDKVNIVGHSMGGVSALRY
                     LGTYGQDTSLPQVQTFTAIGAPFNDFVDDSGQSLTEELAMGPAVVSSRYQDYQQIIGN
                     MPTTTRFFLIAGQLDEADLSDGTVPLNSALAVYALLKQHGNEVEEKIVKGENAAHSML
                     HENQEVDQLVSRFVWNE"
     gene            complement(33899..34444)
                     /gene="nusG"
                     /locus_tag="JZ382_000394"
     CDS             complement(33899..34444)
                     /gene="nusG"
                     /locus_tag="JZ382_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY2426606.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(34545..34715)
                     /gene="secE"
                     /locus_tag="JZ382_000395"
     CDS             complement(34545..34715)
                     /gene="secE"
                     /locus_tag="JZ382_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY2426607.1"
                     /translation="MKFLKSVKDEIKQVTWPNRKQLRKDTLVVIETSILFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(34736..34888)
                     /gene="rpmG"
                     /locus_tag="JZ382_000396"
     CDS             complement(34736..34888)
                     /gene="rpmG"
                     /locus_tag="JZ382_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY2426608.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            35050..35694
                     /locus_tag="JZ382_000397"
     CDS             35050..35694
                     /locus_tag="JZ382_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAY2426609.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKYSSIA
                     SCITLDIEKDEYIRRTLITLPANTEPKVCETIINHLEKKNYKVVEIENLQNQEA"
     gene            complement(35750..36253)
                     /locus_tag="JZ382_000398"
     CDS             complement(35750..36253)
                     /locus_tag="JZ382_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAY2426610.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIIMAVSAPLMYYLDRS
                     LDFEKRV"
     regulatory      complement(36266..36312)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            36852..37814
                     /locus_tag="JZ382_000399"
     CDS             36852..37814
                     /locus_tag="JZ382_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAY2426611.1"
                     /translation="MKFHYHLLINPSAGSGNGHKVAEKILPILKNKHVNYTLHYSEYK
                     GHDAEIAEILAKETLVPWTEEQETIETFPLLIIVGGDGTLHQVLDTFYQMEVEFPVAY
                     IPAGSGNDFARGAGLPKNPKKALQLILAAQSPDKVHIMAYEEKISEKKGIAVNNFGIG
                     LDAAIVHATNHSSTKERLNKYNLGSFSYLFSILRTLFTQKGFPILVDAGGKQLSFSNA
                     FLCTATKHPFFGGGIAIAPTADATKPVIDFVMVERINIFKIIWLILLLMQKKQLKSKY
                     FHHYTTNRLRIVSPTPQYGQEDGEDVAPRPFDLQFSTKNQLFFK"
     gene            complement(38051..40621)
                     /locus_tag="JZ382_000400"
     CDS             complement(38051..40621)
                     /locus_tag="JZ382_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAY2426612.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKVERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKVVELLGEDAIEKIIADPSVLKQIAGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQTYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEILPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGRPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYATCVANESASRMTTLK
                     SRIIDNGKMTITLRTKLAAYEDSLIGNDPFEDGTYTKESQDIKEKLPIKDDSGTNESK
                     NQVMETVEPDLFSSSVVEEKKEEIEPAVPKEFVLTAEAINQKLVDPMIGMENITPYTL
                     "
     gene            complement(40641..41300)
                     /locus_tag="JZ382_000401"
     CDS             complement(40641..41300)
                     /locus_tag="JZ382_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY2426613.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRACDTAEGINKELASPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PENLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKNGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCKLLPYKLKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(41349..41852)
                     /gene="trmL"
                     /locus_tag="JZ382_000402"
     CDS             complement(41349..41852)
                     /gene="trmL"
                     /locus_tag="JZ382_000402"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY2426614.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSDLEAFLTFLGDRKLHLISKFAHKVYSEENFADGEEHFFLFG
                     KETTGLPEAFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(42073..42939)
                     /locus_tag="JZ382_000403"
     CDS             complement(42073..42939)
                     /locus_tag="JZ382_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY2426615.1"
                     /translation="MLKKIDRGRQFLAEKHQLFRCPVCQEEVEKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQEMFVPRRRMIQSGMYQPVIETIAQYLPNEISLLDVGCG
                     EGSFLNELSKLKKIQTAVGFDISKEGVYAATEQSVEAFWCVADLTNLPFADQTFNTIL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNDRVVAKFT
                     ESMKLTERQRVTYEFEIPEENRKDLLAMSPLEWQVEEEIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            43048..43689
                     /locus_tag="JZ382_000404"
     CDS             43048..43689
                     /locus_tag="JZ382_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY2426616.1"
                     /translation="MIKEFCAENYTSIPLAIARGANRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKNIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGVQITFHMAFDSIPEERQFEAIDWLAAHGVDRILTHGGPADQTI
                     EEHFPRLKELIDYADGRLIILPGGGVTAANAAHVAKELNVSEVHGTKIVELQP"
     gene            complement(43749..44594)
                     /gene="mgtE"
                     /locus_tag="JZ382_000405"
                     /pseudo
     CDS             complement(43749..44594)
                     /gene="mgtE"
                     /locus_tag="JZ382_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
BASE COUNT        13559 a         9095 c         7798 g        14246 t
ORIGIN      
        1 ttcttcatag ttttccacaa ttttttctct attagatttg ctattttccc acacgatact
       61 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
      121 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
      181 taagaatgtg tttagaggag gcactcatca tgaacgtaaa aatcttttct aaaaataatt
      241 gcatgcaatg caagatggtc aaacgttttt taacagaaaa tcaaattgct tttgaagaag
      301 tcaatatcga tgaacaaccg gaagcagtag actggttgaa agcacaagga ttccaaagtg
      361 ttcctgtcat tacttccgat gcagaaacag ttatcggatt ccgtcctgat cagttgcgta
      421 aacttgccag ctaacatgac taaaagaatc aaaacaagat acatggattt gaagggcttg
      481 agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc
      541 aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac gtatgagtct
      601 taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc gaccagtcaa
      661 tggtcaaatc cctttgaata aggataaaga agctttggct gccttttttg aagaaaatgt
      721 caaaccaaat acgatgactt ttccatctat tatggaaaaa atcaattact tgatcgaaca
      781 agaatatata gaagcagaat tcatccaatt atattcacca tcatttattg aaaagctata
      841 tcaatttttg gacgaacaac atttttcttt caaatctttt atggctgctt acaaatttta
      901 ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt atgaagatcg
      961 agtagccttc aatgcacttt actttgcgaa cggggatgaa gaattagcac ttgctcttgc
     1021 agatgaaatg atccatcaac gttatcagcc agctacacct tctttcttga atgctggacg
     1081 taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg atatgaactc
     1141 tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg gcgtagggat
     1201 tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatacgagg gtgctgcgag
     1261 tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta atcaattagg
     1321 tcagcgtcaa ggagctggtg tggtttattt gaatgtgttc catccagaca ttattgcttt
     1381 cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc tttctcttgg
     1441 tgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata tgtatttatt
     1501 tagtccatat agtgtcgaaa aagaatatgg tgttccattc tcttatgtag atattacagc
     1561 tgagtatgat cgtctagttg caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga
     1621 tcttgaaaat gaaatctcaa aattacagca agaatctggc tatccttacg taatcaatat
     1681 tgatacagcg aaccgcgaga atccaatcga cggcaagatt atcatgagta atttatgttc
     1741 agaaatttta caagttcaaa cgccttctgt gatcaacggc aaacaggagt atgaagtatt
     1801 aggaacagat atctcatgta acttaggttc gacaaatatc gtgaatttga tgcaaagccc
     1861 taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta cagatgcatc
     1921 tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata cgattggatt
     1981 aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg gctcagagga
     2041 ttcattagac tttacaaata tttatttcat gctgttgaat tattggacac ttgttgaaag
     2101 caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat cagcttatgc
     2161 tgatggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat cagaaaaagt
     2221 caaagcattg tttgaaggga tttttattcc aactgctgaa gattggaata ccttgaaaca
     2281 agcagtaatg aaagatgggc tatatcacca aaatcgcttg gcagtagcac caaatggctc
     2341 aatttcatat atcaatgata cgagtgcaag cattcatcct atcactcgca tgatcgaaga
     2401 acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatttat ccaatgagac
     2461 gattccatat tacacttctg cttatgatat ggacatgcgt aaagtaatcg atgtttatgc
     2521 aacggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc gttctgaaat
     2581 cccagaaggt atttatgaat ggaagacaac aacgaagcaa acgacacgtg atttgaatat
     2641 tttaagacat tacgctttcc ataaaggaat caaatccatt tattatgtac gtacctttac
     2701 tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct aatatcatag
     2761 atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa acatattatg
     2821 aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg gagaagctga
     2881 cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat gattggcgca
     2941 cattgtctca attggaaaaa gatacagtag gacatgtatt cggcggtttg acactgctgg
     3001 atactgtcca atctgaaagc ggtatggatc agttgcgtaa agatgtccgt actccccatg
     3061 aagaagccgt tttgaacaat atccaattca tggaatccgt ccatgccaaa agttattctt
     3121 ctatttttag tacactgaac acgaaaaaag aaatcgaaga aatttttgac tggacgaata
     3181 cgaatcctta tctgcaaaaa aaagctgagc gtattaatga aatctacaaa cacggtacac
     3241 cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat tcaggttttt
     3301 atactcctct ttattattta ggaaacaaca aattggcaaa cgtagcagag atcatcaaac
     3361 tgatcatccg agatgaatcg gtacatggaa cctatatcgg ttacaaattc caattaggtt
     3421 tcaacgaatt acctgaagca gaacaagaaa cgttgaaaga ctggatgtac aatcttcttt
     3481 atgagcttta tgaaaacgaa gaacgttata cagaagaatt atacgatcca atcggttgga
     3541 cagaagaagt caaaacattc ctacgctata atgccaacaa agcattgatg aacctaggga
     3601 tggacccact cttcccagat actgccaatg atgtcaaccc aatcgtaatg aacgggattt
     3661 caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat ttgttaggtc
     3721 atgtagaagc gatgaaagat gacgattatt tgattggatt ggaataagga taaaaaatta
     3781 cgcacaagaa tattctattc ttgtgcgctt tttttattcc acgacaattt gtttttgtgt
     3841 atccgatgtt tgcgaaaaat caatagtaaa gcgacccatc tgctcatcat aggtaattcc
     3901 ttcactcatg ctttgaatag cttcaaaatc atttggtaat tcaatcgtta ttttcgtctt
     3961 tgtatgagca agatcagcaa acgtataact tttttcttgt tcgtttattt tctttaatac
     4021 agaaagtggt ttatctgttt ttaatccatc taattctcca ccagtattat cccaaaaatt
     4081 gatacccaaa taaccggatt cttttacttt tactgcttga atttttggag tattttgaag
     4141 aatagtaact ggttcttttt ctgcatattc ttttaaacct ttttcgttca ctccgggtaa
     4201 tatcacatat gcataagctt cattgattgg atgttttcca tgattgatca aaaacttccg
     4261 ataacttcca tgataaactt catcactagg aaatgattcg ttgatttccc tataattacc
     4321 ttgccgtgct tcacttatca cttttattgt ttctttctca ggaaaataat aaccgatcga
     4381 tgtttctggt tgacttgatt gtaacaataa ccattctttt tcactttgtt cggttggctg
     4441 ggtaatttct cctgtttctg ttacaacttt ataagtaaac tgatcgttta ataaacgatt
     4501 atctataatc gtttcaatag acgcatttgt gttaccggta attcccgaac caagcgcaat
     4561 caattgatcg tcaagcacaa accacgattt tttccctttg acatccattg gcaaaactgt
     4621 cccattattc tttgttccag ttttatttag agccatacct accactgcct gaccttcatt
     4681 tgatacaccg ccaacggctt tctcatttga tcgcacagtc gtaaaagaag agatttcatc
     4741 ttttaaaggg actgtatcga ccgttgttcc tggcaatcga taaggatcga cagtcggcca
     4801 atagctctta ccgaattgta catcatcctc attatataag tagaccatcc catccgatgt
     4861 atgccaacca cgtttgtttt ctttatttcc tgcttcaaaa gaagagatac ggttcgaata
     4921 catactgatt cctaccatat acttactggt acgctgaaca aaacgatcca ttttatgata
     4981 catttttgtc cctatgaatg gtaaatcctt ccctaaaatc gtctggtctt ctaataagga
     5041 tagtgtcatg accagatcat tataatctct tacattcgtt atataatacg aagggttcaa
     5101 ttgcatccaa tatttaactg cttcttttag ttccttttgc ttatcttctg gtgcaaattt
     5161 tgaaacaatc aataaattat acatcgttga agacccgtat cctaccttcg tctttgaagg
     5221 cgcacgcgaa atagcgcgtc cattcaccat gggaagcatt tcaccttggt aaatcaaagg
     5281 aataaaggcc ttctctacat tttctacgaa agcgtgaatg tcatcagaag ctgctgcata
     5341 agaagtccca tccacaatcg taaaaatctg tccgactcct ttgattagta cattcccgta
     5401 agaacctgta taaggaatat cattatgctg gataaaggaa ccatctgcgt aaaacccatc
     5461 tccacttgtc acgagttgaa aaacatcttt gatcccattc actgcttgat ggatttttgt
     5521 ggcatctttt tgtaaaatac ctactcctaa aacagtcaat gataagtctg ttcgattggc
     5581 accagtcgtt gaaaagttag ggataaatgc aaggtctaca aatgaatctt gtggtttagt
     5641 atataattgc tggaatggat caggtacata ttgatttaaa atagctgtat attgttggat
     5701 tttttcactg gatagctctt ggtgcaacag tagaaggata ttcacaaatt tttgtgggat
     5761 accaatttgc caatcccacc aatttccata gtactttttc ccatcatatc cttttttggt
     5821 aatcataaaa tcgattccct ctttaattgc tgcagcaacc tctggctgtt gataaagtgt
     5881 tgtccctttt gtcttatagg cataagtaag tttctggagt ttcgtaaatt gtgtcgtcaa
     5941 atctgctgaa gaagtatttc ccgattcaag tggccataga taggtccggt caggtgattg
     6001 atccaatgtt tgatataaat tttccgcttc ttttgataac gcttctatat atgctcttaa
     6061 tgactcattt tccggacttt ctttttcaaa gacaagttga cttttccatc gttcttccat
     6121 cttttgaaat tcatcttcct cggcaaaggt tgatatactc attccaaata aactaattaa
     6181 actaagcata ctcatcaaaa gaatccttct acccaaacct tttttctcct tctccatctt
     6241 tgctccctct ttctcatcag attgtatgaa tatacagagg ctaaaatcac atttatctta
     6301 gtgaatgaga aaaccgctta acctctactt tttggacaaa tatgtgaacg gacgtatctc
     6361 acttttcctt ttttaagcta aaataccaaa cgcacttaat gcaatggaaa gaatcaaaat
     6421 gatccagatt actcgtgtgg aattcaattt cttttgtccc aacatccaat aagttaataa
     6481 aacaagtaac accggaacaa gagagggtaa aatagcgtct aatgtatctt gaataactaa
     6541 ttccacacca tcttttttat aaacaaatgg gaccgttgcc tttaccacgg aaggaatcaa
     6601 agcaccaatt accgtgacac ctaaaactgt tgcggcatta gtgaatgcag ataatttgtt
     6661 tttcaaagtt gtgacaagtt tgactccttg tttgtatcct aatggtaata acgcagcccg
     6721 tccaaataac aaaacgatat tcgcgatgac ccaaatcaca catccaacaa ttgagccact
     6781 ttgagctaat gttcctgcaa tcgatccaaa aatcgttccc caaatcacat ggaacaatga
     6841 atcacctacg ccagcaagag aacccattaa agctgttttt aaggcaatga ttgtgtcttt
     6901 tgctttgtaa ccatcttctt cttcgattgc cacatcaatc cccatgatca aatttccgaa
     6961 aatcgcgttg gtattgaaga attgattatg tgtacgcatc atatcttgta agtgtgattc
     7021 gtcattacca tagaattttt tcaatactgg taatatccca tataaatagc cagaactcat
     7081 cattcgttca tagttccagt tcagctgact tcctaaaata tagcgccaac taatcgcatt
     7141 cagctcttgt tttgttaatt tctggttatt catccccatc atatcctcct tcaaatgttc
     7201 cattatttgt ggctgtcact gtcggatgtt ctgctttttc actatctttt ttaaatacaa
     7261 taattgcagc aatcacaccg acgattgaga caccaagcat tggcacttgt aaataagcgg
     7321 ctaaaaagaa acccaatagt aaataaggaa tatattgttt tgttggaagg taatgcaaca
     7381 agattcccac acccacgact ggtaacaccc caccagcagt ttttagtcca ttcatcaacc
     7441 attctggtgt attttctgtg atcgttgaaa cagcagcatc accgactaaa agcatcaata
     7501 aaatcggaat ggctcgtgat aatccccaaa gaaaagagcc agataagact aatcgcggta
     7561 tacgattgat tttcatatca tcaattgctc tatctacacg atgcaagaga taaacattac
     7621 aaaagcgagc gactacatca agttgcaaca tcaataaaga tactggaaca gccaatccta
     7681 taccatactc tgcacctttc ccagagataa ccgcaaatac tgttcccaag acggctcctg
     7741 tgaaataatc aggaactgat gccccaccga aagccgcaat ccctagtctc attagttgca
     7801 atgtacctcc aaccatcaat cctgttttta agtctcccat aatcattcct gaaatcatac
     7861 ctgtgatggc tggttgtgtt aatgtatttg aaataagcga atcattgatt gcaataaaag
     7921 cataaaccgt aataagaata atttgatacg ctgctaagtg catactttct tcctcctcat
     7981 ttttatttaa tttctcagtt tatccatgaa gtcaacaact ggatcatttg gtaccaattg
     8041 agcggttaca tttatatcca ttgctgcaat tgattcgaac acatcaattt cttcttctaa
     8101 tactttgata ctttttgtta tgtcttttga tccctcttta taactcatat ttccaacatt
     8161 tacgtgtgtt agctttcctc cggctttcaa ataacgtaag acatctgttg gatttttgaa
     8221 tactaggaat agacgctgtt ttccatactt tcctgaacgt atatttttcg cagcagattc
     8281 aaccggcaaa actgaaagtc tcatcgatgt aggcgctgcc attcttaagg aagatttttg
     8341 taacgaatct gctgctgctt catcgttgat tacaatgata cgctgtgtgt gcaaactggg
     8401 cgcccagatg cccgcaactt gtccatggat caaacgctcg tctatcctga cattcacgat
     8461 attttctttt cctttatatg cctcatacaa ttggctatta tcctttggta ggcttggttc
     8521 tttttcgatt tctgtttgtt tcattttttg cttcacatca acaatgccag ttgttgctgc
     8581 ttggatcaat tcttttaccg tagcagttga atttaacgtt gcttctatga ctaatggtaa
     8641 agacatccct gcaataattt ccacattttc tgattctaga tagttactta atgctacatt
     8701 acatggcgta ccacctaaaa gatctgcaat aatcaaggat gagccgttaa gggctttcat
     8761 tttttttaat aattcttctt cgaattgccg attgtcccca tcaggcgtca acgcaacgac
     8821 atgaacatga tcttgctttc cgacgatcat ttctaaactt gccttgacgc cttcagccat
     8881 actgccatga ctaatcaata taatattctg catcactttt cctccccaaa aataaaatcc
     8941 tacttaacat attatctttt gtacaagcac actataacat agtttatctc attcgtaaac
     9001 ctaacatctt aacatattat gtttttttat ttctgtacaa ttatctaaaa agtatcagca
     9061 tacaaaaagg tgagaggcaa aagtaactct taaacttttg cctctcacct ttttaactat
     9121 ttccttattc tatacttttt ccagttgttt cctcatagtc tcttttcatg atggcccatg
     9181 atttctcaat gtcttcgtct aaaccaaata atccactacg gccgatgata tagatatctg
     9241 gtccggcatg atcaattcgc ttaaatgatt ttcgattcga agatccgtcc atttcaatca
     9301 aataatgata tcccttttct tgacgaagtt ttcttaaatc aacgatttta tctaatgtgc
     9361 tttcgatgaa gcgctgtcca gcaaaaccgg gatcgactgt catgatagtg attttatcca
     9421 ctaaatcaat ataagggaaa atagtttcaa taggtgtttc aggatttaat acgactcctg
     9481 cttttagtcc tgcagcatgg atctgatcaa tcaatcgaaa ggctagccca tctaaacatt
     9541 cgacatgcat acaaatccat tcgcattttt gtgaaatcaa ttgattcacc caaaaacttt
     9601 ggtctgtgac cattaaatgg gcagacattg gtaaatgact gatttttcgc acttcctcaa
     9661 tgaaccatgg agacaatgtc agattaggta cataatggcc atccatgata tcgatatgat
     9721 aagaatcgac atgatgattc aaaaactcaa tttgttcctt aaatttatcc agatccatcg
     9781 tcattaaaga tggtgagaac aaaactttac tcatatataa gactccttta tttaaaaatc
     9841 aatgattgaa tccccgacta aatgaaggac ttagaaaatc tgaatgtctc ccaagtcaat
     9901 atcttcttct tccttttcca ctacggattc gacttctttt cccacatctt ttttaataat
     9961 tgccaaaaca acggctgtca caagaacatt cactaagatt gctatcacgc ctgcccaagg
    10021 tctggacatc gtcggaatca tcaatacacc gccaaagggt actgttgcat cggcgcctag
    10081 gatcattgtc gttgcaccac cagcaaatcc gccaatagcc gtagcaacca cacctcggac
    10141 gatatcattc atcactagtg ggatcacacc ttcgacaata ttgatcacac ccattggaac
    10201 agctgatttc aatgtttcga cttccacttt tgtatagatg tttttccgaa atatttttgc
    10261 gacaaagtaa gctaatccaa acccgatagg cgtcgccgta ttcaccaatt ggagggctgt
    10321 aattggccca ttgattcctt ctgcttgcat tgtgagaaca aaagcaaaaa ccgttttgtt
    10381 aattggtcca ccataatcaa cgccacttag taagccgatc acaccgccaa aaatcaacaa
    10441 ggacgaatta cctaatcctt ctaagaaatt cgtaagaata gacgtaaaca aggcaacagg
    10501 agttccaatg atatagacca tgattaaccc acctaggata gaagcaacaa agggaataat
    10561 caatgttggc atcagtcctt ttgcccaatt tgggactttc acatggttta aaatagctaa
    10621 cacgagaaat ccagaaagat agccacctaa tatccctcca ataaaaccag ccccaatagc
    10681 attcgctgtc aatccgatga tgaaccctgg agcaatccct ggttttgctg caatagaata
    10741 agaaatcccc gttgcaatga ctacaggaag taaccctaaa ccagcgcctc ccattgttgc
    10801 taaagcatca aaaaaagaaa attcaccttg taccagtgtt ccttgacttg tcccaccgaa
    10861 cgccatcccg atagcaatca agaatcctgc tccacaaacg attggaatca aataagaaat
    10921 cgctgtcaat aaatgtccct ttaaatttaa cttttttatc ttttccatct tttttccctc
    10981 cttatgatca agcaagttgt ttttcaatta tttcttgtgc tttttcgatc agtttattgg
    11041 gtgactttac ggcgatttct gtcggtactt gaatgatacg ttttccttca aatcgttctc
    11101 tgccacttat ttttacatca attgccagaa tcacgagatc tgcttctttt atttgatctg
    11161 gggttaattc attctcgatt ccaatcgttc cttgcgtttc gatgtgcatc tcgtgtcctg
    11221 cttttttggc tgcattttct aatttttctt gtgcaatata cgtatgggca atcccaactg
    11281 tacatgcggc tacacctacg attttcatct tttgtctctc cttattttct tttctttaat
    11341 gtttgaataa atctttcgtc agctaaacat ctcatgaaat tcgctatttt cttctttttt
    11401 tccgtagttt cgtttggcga aagcaataca aaaagaatca aatcgacttg ttcgattgct
    11461 tcgttccatt gaataatggt tttttgggga tacaaaatga tgatttttga ttcagtgatc
    11521 aactcatgtt caatatgagg aagaatgatc ccctcttcaa tttgaatatt tccattttgt
    11581 tcacgcataa gaaaagcttc catcaatatg cttattttct cttcttctcc agggtaaagt
    11641 gaacttgcta accaatgata tgcctctcgt tgagaagtaa cggatacttc gattttttcg
    11701 atgaactcat ctttcattat agatttcccc aatctttttc tggattcgtg attgatcatc
    11761 tgctgttagc atcgcactaa caagtaatgt atggactcct aatcgtgagc ggattccaac
    11821 tgttgtcaaa atcaattcga tgttcggatt tttttctaaa gccgtacgat agttttttgt
    11881 cgcaatgaca tctactatat ttaagtctgg aaattttttg atgatttttg ctttcaacaa
    11941 ttcagaagtt ccaatacctg tcgtacacat aatcaatgtc tgaatcggta attgttgtgt
    12001 ttctaacatc cgtgcgaaat acaacgttat gaagccaatc tcatctgcat tgattagagg
    12061 taatttgaag tgctgactga cttctttaga tacttcagac acactgttat agattttttc
    12121 ataggtcagc tgaatttggt ccaacaagcc attttttacg tggatacggt tttttaagcg
    12181 attgatcgtt tgatatgatt ggctagatca ataaaaatac tttcatttgt cactttcata
    12241 ttgattcgtt tgcctacttt ttctaaatag taagtagtga ttttttctac ctcttctgga
    12301 aattttttta tttcttttgt tgttttttcc attcttgagg aaactagata ctgatagaga
    12361 tagtaagatt cattcacaga taacgaagtt gccagatatt tttcaacatt ttgtataacg
    12421 atttcggctg tttttttaat ttggttatct ttttcaaaga attcaatttg ttccgtggtt
    12481 agttcctctt ctctggcggg agcactcctt ttccttgagc gactgatcaa gatatacaga
    12541 tgtgaaaaaa tattgacatt gtaaggataa ggaagtccac cttctaatat tttttcgatt
    12601 agctttaatt gatctgaaac gaacaagaca tcataacgat tgaagttgaa cgttttcgaa
    12661 gcatttattt catctatatc aaggatattc atgcgccaaa taacttctgc aagccctcta
    12721 tgtatatctt cttctaaacc tttgatggct aatgtccgat tttttcgaat caattgaaga
    12781 tcaaacttcc tcaacttttg ggcgatactt ttctcatcat tggaaattgc cgaatcaccc
    12841 acctcgtagg cattgaaaag ctcagatatc ctgattgctc ttggtgaaga cagtaaaagt
    12901 tcctccatta cacgattctg acgttcattt ggcgatagac taattgatag attatttgtt
    12961 tctttgtttc gttcaataaa tttttcgtag tctaatttat accctttccc cttttctgat
    13021 aaaatcaagt gaccattttg aaattctgta tttatttttt ttactaagcg gtagaccgtt
    13081 ttagtcgagg tatctaataa atcagctaat tcttctgaag tcatgtaatg attttgtttt
    13141 gacaatagta gcactaattt attttctcga ttcatttgac tagtcatgat ccttccccct
    13201 ttgtatcttt atcataacca tttttacccc atctgtttca ctgaaatttg tccattgcat
    13261 cagacatttt tgtatccgct ttctaaaatg tgatgtttta tcccaattta cttctattat
    13321 ttttataaag ttccactaaa ttcatctacc gtcataattt tatttacttc tgcaatagca
    13381 gactttatgg gttttaattg atttggatat gttaacatgt tatcctatta gataatgatg
    13441 atgaaagaaa ggaagacttc tagtatgatt cctaaatacg agcaaatcaa gcaagactta
    13501 cttgaagaaa tcaaaaacca ccactttatt cctggtgata gattttactc agaagcagat
    13561 atcaaacgga aatattctgt tagttcaatt actgcagtca aggctctcaa tgaactaact
    13621 gctgcaggct atctctatcg tatccaagga aaaggaacat tcgtttcaaa atcaaaagtt
    13681 tctcaaagtg tgaaattctc tgatatcgaa ctacattctt tagataaaga aaaagttaaa
    13741 gtactctcga ttgaggaaga aaatgaaatg gatattttaa aggaattagg cttaccagca
    13801 aatgcttctt actataaaat taaacgtgtt cgtttctttg aagacacacc ctttttggtc
    13861 cacatcactc acttaccaaa aaaattagtg aaagaaccaa tagcaaaaga tatttctgtc
    13921 tattcaagta tatacgaaaa agttcgtaaa gattttggga ttgatctttt ttcccttgct
    13981 tctgtggaaa caaatgaaat tgttttccca gagcatgcag aactacttaa tctattgaat
    14041 ctaagtttcc gagaacctgt tgtacaacaa atcaaacact cttacttagc agatcaaagt
    14101 gtagcagaat atatcatcag ttataaacat tggaaatact ttaaaacaaa aattgaagtg
    14161 gaagcggaat agctttcact tttttgtttg ctctcttcta agaaactttt ttaaatcctc
    14221 ttgaatactc gctttttttt tctaaatacg ttatactatt tttaagttaa tatgttaaca
    14281 ttttaagagg aggtagctat gaatcttcaa caaaaaaagg aaatgttgat tgaattggga
    14341 acatttgtag ataaaaattg gttggatgaa caatttgaat ggtgcatgat gcgtatcaaa
    14401 aaaaatatgg ttcgtttcgg gttagatttc ccttcagctt gtgcaacaaa cggaaaatat
    14461 cgaatcaaaa aaaatgatga ttggacaaat ggtttttgga cgggaatgct ttggttatct
    14521 tatgaatgga caaaagaaga gaaatttcta gtacgtgcaa tggaaaatat ccatagcttt
    14581 caaaaacgct tagaccaaca cttcatcctt gatcatcatg atatcggttt tctttatagt
    14641 ttatctgctg gagccgggta taaagtcgtg gatagtcagt tgtgtaaaga atcgctttta
    14701 caagctgcga atattttagt ggcaagattt cagaacaaag ggaattttat ccaagcttgg
    14761 ggagcatacg gtgatcctct cgaataccgc ttgattattg attctttgat caatcttcct
    14821 ttgttgttcc aagcatgtac gctttctaat gacccacatt ataaagaagt ggctttcaag
    14881 cattaccaga cattgttgca taccgtgatt cgtgaagatg ccacgacttt ccatacctat
    14941 tattttgact caaacacagg agttccaaca catggtgcaa cacatcaagg tcatagtgat
    15001 gagtcgatct gggctagagg gcaaagttgg gcgattttag ggattccact aaacgagcga
    15061 tacttgcatt ctgctccttt tccgttgaac tacaaagcga tcgtcgatgt tttcttagaa
    15121 catttacctc aagatcttgt accttactgg gattttgatt tcaatgatga ttttccatca
    15181 gataaagaca gttcttctct agcgattgct gcatgtggat tattagaagc agaaaaaatg
    15241 gaagcgtatc ctgaagcaaa agaaatagca aaaggaatga tttatcaatt aggaacacac
    15301 tatactgcta aaaatgatcc agaaaatgaa ggaattcttt tacatggcgt ttatgctcat
    15361 gcggaaggaa aaggagtcga tgagccaaat ctatggggcg actactttta tttagaagcg
    15421 cttatgcgtt tagcaaaacc tgaatggaac acttactggt aaggagaatg aatagacatg
    15481 caaacattta aaataaaaga cgaattttta gtcgacggta ttcctacaaa aatcattagt
    15541 ggggcaatcc attatttcag aataccccct tctcaatggg agcacagttt atataattta
    15601 aaagcactag gagcaaatac ggttgaaact tatattccgt ggaatcttca cgaaccacaa
    15661 gaaggttcct ttgatttttc cggattcaaa aatgtcgttc gatttgtaaa aattgcacaa
    15721 gaattagact tgatggtaat tttacgtcct tgcgcctaca tttgcgctga atgggaattt
    15781 ggtggattac ctgcttggtt attgaaagaa cctaatataa gagtacgttc taccgatcct
    15841 cgatttatgg aaaaattgaa aaattattat caagtacttc taccaaaact tgcacctctt
    15901 cagatcacac aaggtggtcc agtcatcatg atgcagctcg aaaatgaata cggttcttac
    15961 ggaatggaaa aatcttattt acgccaaaca aaagaattga tgcttgcaca ttcgattgat
    16021 gttcctttat ttacctccga tggtgcttgg ctagaggtgt tagatgctgg tacattaatt
    16081 gatgaagaca tttttgtgac tggtaatttt ggaagccact caaaagaaaa tgcgcaggtc
    16141 ttaaaagaat ttatgcagaa tcatcaaaaa aattggccga tcatgtgtat ggagtattgg
    16201 gatggatggt ttaatcgatg gggagaacct atcattacgc gtgatcccga agaattagca
    16261 actgaagtaa aagagatgtt agaaattggt tccttgaatc tatatatgtt ccacggggga
    16321 acaaattttg gtttttataa tggatgttct gctcgaggca atacagatct tccgcaaatc
    16381 acaagctatg attacgatgc actattaaac gaagctggac aacctactga gaaatattat
    16441 gcagttcaaa gggtcatcaa agaagtttgt ccatctgttt ggcaagcaga gccccgaaca
    16501 aaaacgttaa aaaatctagg gacttatccg gtaaatagaa gcgtttctct atttcatata
    16561 aaagaacaga tctgtgaaga aatcaaaaca gattaccctt tgacaatgga acaagcaagc
    16621 aatggctacg gctatttatt atattcactt accttaaaaa attacggaca taaaaataaa
    16681 ttacgtttaa tcgaaacaaa cgatcgtgca caaatatata ttgacggcaa atacgaccaa
    16741 acgcaaacac aagaaacact gggtgatgaa atgatgattg aaggacaaaa aaatcagccc
    16801 actatcgcat tagatgttct agttgagaat ttaggaagag ttaattacgg tgcaaaattg
    16861 aatagtccct cccaatcaaa agggattcgc aatggtgtca tgcaagatat ccatttccat
    16921 cttggatacc gccattaccc attgactttc gaacaagctc aactagataa aatcgactat
    16981 tccgctggca aagacccgtc acagccttct ttttatcaat ttgagtttga tcttgctgaa
    17041 gaagcagatg cttatatcga ttgttcttta tatggtaagg ggatcgtcat cgtcaatgga
    17101 ttcaatctcg ggaggtactg gaatcatggg cctgttttat cactatattg tccaaaagat
    17161 gtactcaaaa aaggcagaaa tgaagtggtt atttttgaaa cagaaggtat atcaattgat
    17221 acattgattt tttcagatca accaataaaa aaagatttgt catgaacgat ccaaaataat
    17281 catcctgtaa attagcttat acaaaaaggg agtcctacga aaatcattgt tcgattttca
    17341 aagcggattc ccttatatag tgaatagctt ttctattatt atttctctta atcaagtaca
    17401 agtgattact tctcgtccga aaatctcaat ttgtgttaat gctgggaatg gtgacgtatc
    17461 ttctgctttt ttcaaatttt tcaaagtaac tgaactcgtc attttttcag gaaagtaaaa
    17521 cgattgaaat tcaagcgtat ctgttgttgc tacttttatt gtttctccat cagaaaaaca
    17581 aatagaaact tctgtccaat agctatcatg tggataatct cctcgaaaca aaagatttac
    17641 tcgatcggtt tggattgagc gtccaaagtc gatcgtcaaa gttgcatctt tttgctgatt
    17701 gattccccaa gaagtaaatg gataggatcc atgtgagcgg ttaccaaact ttccgtcaat
    17761 cgcattttta gcaaaaaaag tcacatcatt tctcgtctct acattagcat atgcatgtgg
    17821 atatgctcca ctgaacaatt tttgatcatg agggtttgta gatacattcc gatagctttc
    17881 cacttcaaaa tcataggctt tacgaacata tatgtgatgt ctttttgaac gaaatcccgt
    17941 ctctactgaa gctttcgttt cttctagcgg aatcacatat tcccatttct tctctggaat
    18001 ataaagaagt gttggtgcca atgtttcatc taattgcaca acaaaatagt aatcattttt
    18061 ctctttcgtt atgaccagtt tgtctcctac ttcatatgtg tgatttttga ttgctaaaac
    18121 tgctatttct tctccatttg cagaaaatac ggtttctttt ttctcgtgat cgattttgcc
    18181 aataactact tgattatttt tattttttag atcaatttgg atcatttttt tctcctccat
    18241 ttattattcc gccaactaaa caaataaagc gatgggtgcc aggttcgatt ttctttttca
    18301 aatagggaag attggttctt ggaaagaata cagaagtatt tggttctagt acctgaatac
    18361 ctgcttcttc atacccctcg attgcaatga tttgagacac atgcgtttct tttatttttg
    18421 cacagtcttt cgacaaagtt atttctggtt cttttaccaa aagactaaat ccgccttctc
    18481 ttatctctaa aggaatcgtc gtatatatct catggaatcg aacatgccat tcaccaaatg
    18541 ggtaaatttc tgtttggatc agcacttctt ggaatggttg ccattcatac ataacaaaag
    18601 tctctgttgc ttcactactt cgtacctttt tctttgtcct gaaatattca ccgtctcttg
    18661 aaagagctaa tgtattatca aatgcccctt cttcatatgt gattcccgct tttggcacac
    18721 taaagccaaa tttagtagag tagacaaact tactatattt cgctagggca tgcgcttggc
    18781 catcgatcat caaaccatgt ggataaccaa gtacatgttt tccttctttt gcctgacaca
    18841 aaagcatatt tccattatca atcaatcttt tctctttggt attgactggt tcaggtttgg
    18901 cttcccaata cggatgttct tttttagttg ctagtaataa aaatactttt aatgcccaat
    18961 agggagaccc cggtgcgtta taaccttctg ccatcacaag gttctcataa tgatacccaa
    19021 tggatagccg tccgtcataa gtaaagatat cgtgtgacat ccactgcttc atatggttgg
    19081 aaagaagcgt ctttatctga ccccaaggga ttgcttccac ttctgcaaaa atcaatgcag
    19141 aaaaaaaagc accttgtgca aatcgataga tgagacttcg tccataaggt aacgcttctc
    19201 catcggagtc gaaataataa atatagtcct tcgtaaactc ttttgcacga tagatgaatt
    19261 tttctgacca ttcaggaaag tcgtctttca ttaacttggc aaaaatcaat ccataataat
    19321 gaaaagcaaa aggaacatag taatcttttt gcgaagattt accgtcgata taccagcctt
    19381 ttcctacata catcgtatca ataaagcgta attctgcttc cattttttct ttatctaatg
    19441 attcaccaca atgttttaaa gcgattcgga tcaaaatttt gaaaaatgtc caattatttt
    19501 taggtatttt tttatctaag gcttgtttta gccaatgaat caactgttgt ttggaagtgc
    19561 ctgacagttc atcaaagaaa tagtttcgat tcaataacag attcaaagat aacgctgcca
    19621 tttctacgat atattggtca taatcgttaa tatctcccca ataaaaagaa tcttttggat
    19681 tcatcccttt caagattttc tttcgataga cgtctttcaa ctcatctttc gtctcatggt
    19741 tcatccaata aggtgccaat ccccataatg gacgaataaa tgcctccatt tctgctctct
    19801 tctcgtcata aaccgcgccg ctactcccta gacgtacccc tgttttttca ctattgaata
    19861 gttcctgttt taaaggcaga attatctcct ctagtgcttc ttcaaaatcg cgccgcgtca
    19921 aaaatagatt ttgttttaac tgattcatga caaccctccg aattcttttt ctttaggata
    19981 tcatggtaga aaatcggttt caggagagac ttctgagtga gatgaaaatg aaatgagtga
    20041 gaaaacagga tagtcaaaac ttaatatgtt aacacactta gtattgtttt ctcttggaag
    20101 catttcttgt ggtttcttcg ttttacttac tctatctaaa atgaaagaaa tctaatatac
    20161 ctttgagcca ttttcagtaa acaatattta ccccatgatt tgctcattcg tccaccctaa
    20221 acaggacgga tcgattagat tataaaaaag catcaactgg atcatataaa taagaatctc
    20281 aacgtacatc taaataaaat gaacgctaag atcccttttt aaataggctt attcacttgt
    20341 ttcttccagc acttttggac tatctaactt ttcagaaaca tgttccattt ttctcaataa
    20401 atagattttc cgaatattta atattattgt cttaatcact gcatagacag gtatacaaat
    20461 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcag
    20521 tggatggata ttcattgatt ttccaaacaa caacggcttg atcaaatttc catctaattg
    20581 ctgaatcact aaaatcgaca acgccatgta caaagcttga aatggagaga taaacaaacc
    20641 gacaatgatt gctggtactg ctccaataaa agggccgaca tatgggataa tattcgtgat
    20701 gccgcaaatg accgcaagca ataagctata aggctgattg aatattctca ttgccaaaaa
    20761 gctcatcaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    20821 ttcatttaat tctttcactg tttgtcgcaa ctctttacga atcgctttcg gaaagaaatg
    20881 agacaacgca tcaataaatt tgtgtccatc cttgaacata aacagcaaaa taaatggaac
    20941 cgtaaataac aaaataaaaa attgcatcat gaatgaaatg attttagtga cacttgcaga
    21001 taagctgacc aatatgatat tgaacaaatt actgagagat aaattcgctg atgctaattg
    21061 ttcttccaaa ttgaaatttt taaactcttc tcgctgcacc aattcattta gccatttgct
    21121 tgtttcatct gcatagattg gcaggctctg agtcaattga atcgtctgct ccactaactg
    21181 aggtactaca ttcatgacag ccaaaacaac taaaatgatt acgaccaaaa atgtcaataa
    21241 aaaacccaac actctcggaa tccgatgttt ttcgagaaaa acaacgattg gatcgaacat
    21301 ataatagata aaaccagcaa caagcaacgg caaaaaaata gccgaaagca ttttcaatat
    21361 tggactaaaa atattcggca tttgtaaaat gaaatacaca ccgacgatcg tgagtactag
    21421 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac ctctcctcca
    21481 agttcctttg atacttctat tctatcattt aacaaagaat tacgcgctga gattttcatt
    21541 tgcttaaaaa aaacaaaata aaaaaggaaa ggtattttac tgatcagaag agtacttcaa
    21601 caaaaacacg atccgcccaa aataaaaatc gttttgggca gatcgtttat tgcgcaatac
    21661 tgactaaatg tagctttgtg atatttttgt gcacggacaa ttcctcgatc acatcggcat
    21721 aagtgagtcc tttaggaaga tcgatcgtat atatattttt atatacacga tagtcatcaa
    21781 cgaatttcac atcaaaattc acgtcttcga tcacgatatt tttctcttca aaataatcgg
    21841 taagaaagtc tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag
    21901 gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga
    21961 tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccggcaata gcccataaag
    22021 aagcggcagt cgtaagtccc gtcactgatt gtttcgtgac gatgatcgtt cctgcaccta
    22081 agaaaccgac gccactcact acttgagcaa tcagccttgc ttcatctgaa cggatcgttc
    22141 cagcaagctg tggatacgct aaagcatctc ttaatgctcc actagcgatc tcgacttgga
    22201 tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcatc cctgctggac
    22261 gatttttata ttgtcgctca aagccaattg ctccgccaaa tagcatagcg atccctaaac
    22321 gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa
    22381 tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaga
    22441 gcgtgggaca aaagtaaaga aaaacttttg tcacacactc taaacatgtg taaacggcgg
    22501 aagcagaagc aactccttcg gaaataagct gaaattcaca gaaatttgaa aagcaatttt
    22561 cgtgaattcc ttcttatttc ttggagttaa acgcttctgt cccagcctct ttggtaaatt
    22621 acaatttact tgctgcttct tcatcgatga tcacaacgac attgtcgtgg ttttgcaata
    22681 cacttgctgg taaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt
    22741 tgctttcgcc aaacgccatc aagacgattt ctttactttt catgattgag ccaattccca
    22801 ttgaaacagc ttttgttggt acatcttctg ctttatcaaa gaatcgtttg tttgcattga
    22861 tcgttgattc tgtcaaatca actactgaag ttgttccgtc aaatgaagca ccaggttcat
    22921 tgaacccgat atgtccattt tgcccgattc ctaaaatttg gatatcgatt ggatgagcat
    22981 cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttc ccatctggta
    23041 caaatgtttc cttgaatggt tttttatcaa ataattggac atccatgaaa tggcggtagc
    23101 tttgttcatc tgaaccacct agtccaacgt actcatctaa gtttacagaa gtcatttcac
    23161 tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg
    23221 ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt
    23281 ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttgtgtt
    23341 ttggtatagc ccaattatag cattcttttt ttctttttac aataatcagt atagaccaaa
    23401 ttctattttt ttcattttct tgacaacaaa agggcatccg tgttaggttt cataagatgg
    23461 ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaatt gattcactgt ggacacttta
    23521 tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatc
    23581 acccagcagt tcggcgataa aatcctctac gaaggggtcg atctgcaatt aaacgctggc
    23641 gagcatcttg ggctgatcgg acaaaacgga gccggcaaaa gtacattgat taaaatcatc
    23701 actggtgaga tcctgccaga tgatggacag atccattggc aaaaaaatat tcataaaggt
    23761 taccttgatc aatatgtaga agtcgatgaa acattatcga tcgaagaatt tctaaaaact
    23821 gcgtacgcaa aagaatacga aaaagaagaa aaaattgctt ttctttacca aacatatagc
    23881 gagaccttcg atgattcctt gctagaaaaa gctgggaaac tccaaacgga attagaccaa
    23941 ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat
    24001 gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt
    24061 ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac
    24121 ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat
    24181 atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc
    24241 gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa
    24301 caaaatcgtg aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagact
    24361 gaagcttata ttcggaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa
    24421 aaacaattag ataaaatcga acggttgacg cctcccgctt cagcaagtaa agctgtcttt
    24481 gattttccat tctcgcctat tgtcacgacg ttggcagtgg aaacaactga tttagtcatc
    24541 ggctatgatc gaccgctact tgcgcccatt aatttgacca tccggtttgg tgaaaatatt
    24601 gccattcgag gcttcaacgg gattgggaaa tctactttgt taaaaacatt gattggtgag
    24661 atcgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca
    24721 caagatctat actgggaaaa tccattagaa acaccactgc aatacttaag cagtcaattt
    24781 ccaaaagcga ctatcaaaga attacgtcgc caattagcta aagctggact agtcaatcaa
    24841 ttagcaagcg aacctctcac tacactaagt ggtggagaac aaaccaaggt gaaacttgca
    24901 cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatattgat
    24961 caagaaacaa aagaaagctt acaagatggc attcggaaat ttccaggaac ggtaatcatc
    25021 gtctctcatg aacaagaatt ttatcaagat cttgttcaac gagtcattga gatcgagcca
    25081 aagtaatgag acaaaaagac aaaaaaactg atgatagcat gatcatcagt ttttttgtta
    25141 ttcagttttc ttttttctct cgaaaaacaa cgaactgaaa gaaaaacgca catccctgaa
    25201 aaacaagtcc gctaatcgtt acacctaacc aaccatactg ttgccacatg gtcgtcccta
    25261 gcaatgaacc agccgcacct ccaataaagt acataaacat aaatacagta ttatttcggt
    25321 tgcttgctgc ttcacctagt ccttgtactc gtgtttggtt ggcgacttga ccaaattgat
    25381 tcccgacgtc taataaaata atagctaaaa gcaaaatggc caaatggctt ccgccaacca
    25441 ataaagcaat aaaacttatt gtttgcataa caagtcccat atatacaatt tttctttcag
    25501 aataagaatc tgccagtcgt cccacgatag gtgcagcgac tgctccagac aacccaaaaa
    25561 tcgctaatat ccctgcttca aaagtccccc aattgtaagc gggactgctg atataaaaaa
    25621 tcaacgtaga ccaaaagatt gaaaatgtgc caaacataaa aaatccgctg actgccgctt
    25681 ctcttaataa acgttgagag gcaactaaat gcggcaaact tttgagtgaa tcaaggtatg
    25741 aaacagatgt actactttgc tggacagaac gcggcatctt ccagtaaagc agaagtgtaa
    25801 gaatcccgac ggcacataaa gcaaataaat agatcatccg ccaagaaaaa agactggcaa
    25861 caataccaga tacagtccga gaaagcaaaa tccccacgag taaaccgcta agcaatgttc
    25921 ccatgacttt tcctcttgct tttggtcctg ctagtaccgc accgtaagga atgataattt
    25981 gcggcacgat cgaaagtaat cctattaaaa aagaagcgca tgcaaatgac gcaaacgaag
    26041 gcgcaaagaa agcggccaac agcgagatag cagataacgc tgccattcgt ataattaatt
    26101 ttggacgatc caccacgtct cctaaaggaa ctaaaaacag tagccctagt gcatagccca
    26161 actgggtcaa cattgtcaat atcccgactg cacttgtgct aacagataaa ctagctgcta
    26221 cttttgttcc aatcggctgg atataataca tattggcaac aacgacgccg caagtgattg
    26281 ataataataa aataagcgat gttgttaaag gtgttttttc tttactctcc attgatttcc
    26341 cccatttcct atcttagttt acaacttatg gaaaagattg gaaatagaaa aatacaacta
    26401 ttcgccatac ttattatttt tgtctttgtt ttccttgttt tttcttgcga tctttataaa
    26461 tgaagacaac taaccctact aacacagtaa cacttgcaac caacgtgact gcagttttgt
    26521 tatatccgtg aaacagagct tctcccccac acgcatacaa cacagatgaa gggatgactc
    26581 caatcacgat cgacattacg atttgctgaa gaggcaattt catcgcatct gcagcaaaat
    26641 tgaccataga agatgggatc accggtatca tgtaacctaa catgacgcct attttgggat
    26701 gtctcatttg ccgaatggat tgcacccaat gatttttttc agttttcttg tctaaaaact
    26761 tcaatcggtg catcaaaaac acagcggaaa tattgcccag agcattccct aaaacattga
    26821 tacctgcccc aataaacgga ccaaagctca atccggcaat gacaccaata accgaagtag
    26881 ggatacccgg aacagcacac atcaccgctg tcaacacaat caagagtagc gatgcagaaa
    26941 aaccatgaga acgaatatgt gcaagcagct cttccttact tgctctaggg tttaaaaata
    27001 attgtatatc tgtctgatat tccaaataca accgatagcc caccataagt atcagaacga
    27061 ttcccaacgt gatcaaaatc aatcggattt ttttcttttt catattacct cctgaaaaaa
    27121 actatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa tccaagatag
    27181 gatctcagtg cttttaaata gttaaaaaat tatttaacag ttacagatgc gccaacttct
    27241 tcaagtttag ctttcaattc gtcagcttct tctttagata cgccttcttt aactggtgca
    27301 ggagcgccat caactacagc tttagcttct ttcaagccta agccagttgc ttcacgaact
    27361 gctttgataa ctttaacttt ttggtctcca gctgcagtta attctacagt gaattctgtt
    27421 tgttcttcag cagcagcagc gccaccagca gcagctacag ctacaggagc agcagcagat
    27481 acgccaaatt cttcttcaat agctttaact aggtcgttaa gttctaggat agttgcttct
    27541 tttaactcag caacgatgtt ttcaatgttc aatgccatta taatttcctc cgtttaaatg
    27601 ttctttttta tttaatactt tatgcggcaa ttaagctgca tctccatctt ctttgtctgc
    27661 cactgctttg acagcgtaag ccacgttgcg gactggcgct tgtagtacag atagcaacat
    27721 agatagaagt ccttcgcgat ttggcaattt tgccaatgct gtgatttctt ccaatgaaga
    27781 tactttacct tcgatgatac cgcctttgat ttctaatgct tttgcttctt tagcaaattc
    27841 gtccatgatt ttcgctggtg caactacatc ttcgttactg aatgcaacgg ctgtaggtcc
    27901 agtgaatact tcgtccatgc cttcaaggcc agctttttta gctgcacgtg aaagaattga
    27961 gtttttgata actttcattt ctacgttagc atcacgaagt tgtttacgta aacgtgttac
    28021 ttcatcaact gttaatccgc ggtagtcaac gactactact gatgcagctg ctgtaaattt
    28081 ttcgcttact tcatcaacga ttgctgcttt ttttgcgata attgcttcac tcatttttga
    28141 gtttcacctc ctgaattttg atggaagagt tttttgaact gaaaaggcaa atcacttgta
    28201 tagtttcttt gctcaaccag tattgttcat catctattct cttcgttcat agtgattcta
    28261 ctcgctttgc tcgaaaggaa actcctttcg ctttgctcaa ccagtattgt tcatcatcta
    28321 ttctcttcgt tcatagtgat tcacaataaa aaactctatg ccaccgcaga catagaggga
    28381 ctattgaata tattcaataa acgtcctcgg taggaaatta aggcttggcg ccacctactg
    28441 tcttcggtac aagttaatta ttaacctcaa ctagcttagc atgctaaact agttgagtca
    28501 agaattattt ttcagttgga tcaatcaacg ttctaatatt tgattagaaa gaagcttggt
    28561 caacatggat accaggtcca aatgttgttg tgatcgttac atttttcatg tattgacctt
    28621 tagctgctga tggtttagct tttagcaata catcatggat tgttgcaaag ttttcaacta
    28681 atttttcatc atcaaatgaa acttttccga ttggtacatg gatgtttcca gctttgtcta
    28741 cacggtaagt tactttacca gctttcactt cgtttactgc ttttgttaca tccattgtaa
    28801 ctgtaccagt tttagggttt ggcattaagc cttttggtcc taatacacgt cctagacgac
    28861 ctacagtagc catcatatca ggtgttgcaa caactacgtc aaagtcaaac catccgtttt
    28921 ggattttttg aaccatgtcg tcgtcaccta cgaaatctgc accagctgct tcagcttctt
    28981 ttgctttgtc tcctttagca aaaactaaaa cagtttgtgt tttaccagtt ccgtttggta
    29041 atacaacagc accacggatt tgttggtctg cttttttagg gtctacgttc aaacggtatg
    29101 caacttcaac tgttgcatca aattttgcaa tgtttgtttc ttttgctaaa gctactgctt
    29161 cggcaacagg ataaacttta cttgaatcaa ctttttttaa tgcatcttgc atttttttgc
    29221 tctttttagc cattttggtt cctccttgat tgtggttata acggttgtac ctcccacgtg
    29281 ttctatatct ttcaatatag agccaactga gaagctgctg tcttagggta tagagattat
    29341 tctacagtga tccccatgct tcgtgcagtt ccttcaacca tgcgcattgc tgcttcaacg
    29401 ttagctgcgt ttaggtcttc catttttaat tcagcgattt ctcttacttg atcgctagaa
    29461 actttcgcaa ctttattttt gtttggttca cctgaacctt tttcgatttt agctgctttt
    29521 ttcaatagaa cagcagctgg tggtgttttt gtaacgaatg tgaatgaacg gtcttcgtat
    29581 acagaaataa caaccggaat gatcaaacct gcttgatcag ctgtacgagc gttgaattct
    29641 tttgtgaatc ccatgatatt gatacccgct tgacctagtg caggacctac tggtggagct
    29701 ggagttgctt tccctgcagg aatttgcaat ttaacgattt tttctacttt ctttgccacg
    29761 agacatacct ccttaagtcc gtgatgtggt taattggaga tgcatatttc tcctcccacg
    29821 ttttatctga tctgaaaaaa tccagacaaa taatgtgcaa aacaatgcac actggtacag
    29881 tatagcaaat ttatttgttt tttcaagatt tttttctgct atttttacat tttgcttgaa
    29941 taaacgctaa atttattcta aaaaaacaac aatttttcgt tcccatttct tcttttatgt
    30001 tacacttgcg ctagcatggt tcaacagatg aaagcaaaca ataacacggt tttttagacc
    30061 cttgtacaaa gattcctaca taacaaaatg aggtggagaa aatgaaagat ttaaggtata
    30121 gaagtgtctt tgatattatt ggacctgtga tgattggtcc aagtagctcc cacacagccg
    30181 gagcagctag aattgggaag atcatacgca gtatatttgg cgagcaacct gattctgtag
    30241 atatttatct atatgaatca tttgcaaaaa cctatcgagg tcacggaaca gacattgcat
    30301 tagttggtgg tctattagga atggaacctg atgatgaacg tttagctgat tctttgaaat
    30361 tggcatatga ggctggcatg gaagtcttgt ttgttcctaa gagtgaaaaa gccgaacatc
    30421 cgaattctgt aaagatgctt ctaaaaaaag gaacacgaaa gctttcagtt actggcattt
    30481 cgattggcgg tggcaatatc cagatttctg aattgaatgg attcaaaatt tctttaagca
    30541 tgggaacacc taccttgatc gttgttcacc aagatgttcc agggatgatc gctaaagtga
    30601 ctggtatact ttctgaaacc aatacgaata tcggtactat gacagtgaca cgggaatcaa
    30661 aaggcgaaag cgccatcatg atcattgaag tagataatcc tcaagttgaa gaaacagtaa
    30721 gaagactcaa acatctgcca aatatcgaca gcgtaaacta tttcgattaa ggagctgaaa
    30781 aaatgttttt atcgattgaa gaattagtag accaagcgaa agcttattca agtgtagcag
    30841 aattgatgat tgcagttgaa atggaaatga gcggccgttc ccgtagtcag atcattgcga
    30901 ttatggaaaa aaatctttca gtaatggaac aatcgatcaa cgagggaaaa gaaggtgtca
    30961 cctccgtcac agggattacc ggtggcgatg caaaaaaatt agatgattat ttgaacgaag
    31021 cagatttttt gagcggagaa acgatcttaa aggctgtacg caatgctgta gctgttaacg
    31081 aagtcaatgc aaaaatggga ttgatttgtg ctacgccgac tgctggaagt gcgggggttg
    31141 ttcctggtgt gttaatggct gtccgagatc ggcttggttt gtcacatgaa cagcaattgg
    31201 aattcttgtt tactgctggc gctttcgggt tagtaatcgc gaacaacgct tctattagtg
    31261 gcgcagaagg gggttgtcaa gcagaagttg gttctgcaag tgcaatggcg gcggcggctt
    31321 tggtttgtgc aaaaaacggg actcctcatc aagcagctca agcagtagcc atcacattga
    31381 aaaatatgat gggactgatt tgcgatcctg ttgcaggttt agttgaagta ccttgtgtca
    31441 aacgtaatgc tttagggtcc tctcaagctt tcatctctgc tgacatggca ttagcgggta
    31501 tcgaaagtgt cattccaccg gatgaagtcg ttgctgcgat gtatcaagtc ggacgacaaa
    31561 tgccatcgat tttcaaagag acagcagaag gcggcttagc tgttacgcca actgctaagc
    31621 gacttacaga agagatttta aataagaggt cgtaaaaaag ctgcctagtt tcgagtatta
    31681 agagattagt tatttctatc acaaaaaaac gaagagacgc acagttaggc ctctcttcgt
    31741 tttttttatt aatccatcaa tttcgtattt tttacttcat cgatcgattt tgttcctgca
    31801 agttgcatcg tgatcgacaa ttctttgttc aggtgttcaa aaacagaggt tactccttct
    31861 gccccaccta gattcaatcc atagattact ggtcggccaa tcgcaactaa gtcagcacca
    31921 cttgccaaag ctttaaagac atgttctcct cgacggacgc cagagtcgaa aatcactggt
    31981 acacgtttat tgactacttc ggcaattttt ggcaatactt caaaggaagc aggacctcca
    32041 tctaattgtc gtcccccatg attagatacc cagataccat ctgcccctgc agagatagcg
    32101 atgactgcat cttctggcga ttgtattcct tttacgatga ctggaagatt ggtgatttct
    32161 ttgattgttt tgatgtcatc tggtgtcaag ccttgttttg cagctgcata gatttctgca
    32221 attccttttc cttcgccatt cccacttgca ctttgttcgc tataagcagc taaattaggc
    32281 attggtaatg gaaattggaa ttgattgatc acatcttctt cgcggtaacc accgagcgtt
    32341 gaatctgccg ttaaaatgat ggctttagcc cctgcttcta ctgctttgtt caaaatgaac
    32401 tcattgaacc catcgtcttt actcatatat aattggaaaa actgcggtgc acctggcgct
    32461 gcatctgctg catctttgat tgtcgtattc gcataagtgc tgatggaaaa gattgatcca
    32521 gcagcagcta cccctttagc tgtatcagct tccccttttt catgcgccaa tccttgggcc
    32581 gctgatggcg cttggataat cggtgtgtct aattcaattc caaaaactga tgtatgcaaa
    32641 tctgctgagt cgattccgcg taatatccgt ggcatgattt tttttgcatt aaaagaagtc
    32701 gtattttctt tcatcgtcca ctcatcttca gaaccaccgc gaatatagcc aaaagctcct
    32761 gcttccattc gctcttttac acgaccttct agtgaagcaa tgtttacaat atcgattgga
    32821 tgttcttcat tacttgtatg ataagtcata gtagatcctt ctttcatctt cttgattaca
    32881 tgtaaatcat actattctta caaaaagtaa gcaaacattt actctcggat ccttttcttg
    32941 ctattcattc caaacaaatc gactaacaag ctgatctact tcttggtttt catgaagcat
    33001 actatgagcg gcattttcgc cttttactat tttttcttct acttcatttc cgtgttgttt
    33061 caacaacgca tagacagcta atgcactgtt taacggtact gtcccatcac tcaagtctgc
    33121 ttcgtccaat tgtccggcaa tcaggaaaaa tcgagtagtt gtaggcatat ttccgattat
    33181 ttgctgataa tcttgatagc ggctagaaac aacagcagga cccattgcta actcctctgt
    33241 cagagattgc ccactgtcat cgacaaaatc attaaaaggt gcaccaattg ccgtaaatgt
    33301 ctgaacttgc ggcaatgacg tatcctgtcc atatgttcct aaatagcgta acgcgctaac
    33361 tcctcccatt gaatgtccca cgatgttgac tttgtctatg ctatagtttt ctttcagata
    33421 caaaagacaa gatttgatcc attctgcttg attccactcg ttgtttttat tatctgcaaa
    33481 caatacttgg accatcggat tgtctgcttt tccagataag tctccttctg cttgtatttg
    33541 tccatcggct tctactatta gaaccaattc ttttactgct ttttgatttt tttccaaacg
    33601 ttgcaacatc cctttaaagg aattttctgt cccgctgtag ccatggaaaa aaagtgttgg
    33661 aatagtggtt ttttctatca ctttactgga ttctaccgtt ttttctttat cagaaactgc
    33721 agtagtcttc gtcgtctggc aaccacttat cgcaaacaag ctgataagca ctaaagctgc
    33781 tgtagccgca tgtttgcata catttttgat tcgatttgtc attcgcctct tcctttccat
    33841 atagaaaaaa gaaagacttg aaaggtcaat cgtatgatcg acatttcaag tctctttatt
    33901 aatcgatttt atctacttga tcgaaatcaa gttctgtact tgtttcacga ccaaacatat
    33961 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    34021 cagcaaatgc gccttcgatg attttcacaa cttcgccaac ttctacttct aaatcatttt
    34081 gtcgtagact catgccgata gaacgcaaga tatgattgac ttcttctgga agtagtggtg
    34141 ctggtttgct tccagctccg tgtgaaccaa cgaatcctgt cacgcctggt gtgttacgga
    34201 caacatacca ggaagcgtct gtcatgatca tttcaacaag aacatatcct ggaaatgttt
    34261 tatgaacgat ttctttctct ttcccatttt tgacttctgt ttctttttct tcaggaacaa
    34321 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    34381 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    34441 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    34501 ctttcgcttc attatagcac taaaagcacg ttgtggctag cgagttattt taagatccag
    34561 ccaaataaac tttggattgc tgtatccatt acgtaaaaca tcaacccgaa aagaatagaa
    34621 gtttcgataa ctaccaacgt atctttacga agttgttttc tgtttggcca agttacttgt
    34681 ttgatttcat cttttacgct ttttagaaat ttcatgagac accctccttt gctgtttatt
    34741 tcgtttcttt atgtaatgta tattgattac aatatttaca aaatttgttg atttctaaac
    34801 gttcaccgct ttttccttca ctgactgatt ttgtgtagtt acgagaccca caaacggaac
    34861 aagcgagtgc tgcttttttc tttgccattt acttctgccc ccttgctgag aatcagactt
    34921 atacttaata acagtatcac gtaacttttt acttgtcaat ttgtgaaata aactatctat
    34981 tgttttagat aaaattatga tatgattaat ttgaaattaa gctaaaatga gacaattgga
    35041 ggtatagcga tgctattaaa aacacttgta tataagaaaa aagatctgac aaccgttaca
    35101 gagactagta cattagaaga agcattaaaa attttagaaa cttccggata ccgttgtgta
    35161 ccgatcttag attcttcagg gaatattttc cgtggaaata tttacaaaat gcacatctat
    35221 cgtcataaag caaacggcgg tgatatgacg cttcctgtca ctcatctttt gaaaaatgcc
    35281 acaaagttca tttatctgga tacttctttc ttcaaagtat tctttacgat caaagagctt
    35341 ccttatattg ctgtccttga cagtgaaaat catttttatg gtattttgac acatagcaca
    35401 ttacttaata tgctctctca atcttggagc gtagaacaag gaagctatgt gctgacaatt
    35461 gcttctacag gaaagcaagg agatctggct gctatcgcaa aaatcatttc aaaatacagt
    35521 agcattgcca gctgcattac tcttgatatt gaaaaagacg aatacatccg acgtacattg
    35581 attacgctgc cggcgaacac tgaaccgaaa gtatgtgaaa cgatcatcaa ccacttagag
    35641 aaaaagaatt ataaagtggt agaaatcgaa aatttgcaaa accaagaagc ataataaaaa
    35701 tgacaaaaga aatacagctc agctgctttc ttttgtcgtt tttttacaat tagacacgct
    35761 tttcaaaatc aagcgaacga tctaaataat acatcagtgg tgctgaaaca gccatgatga
    35821 tgaactcgct taatgcaagt gtaccatagg ttgtccaaaa tgcttggcca cctgtcggaa
    35881 ctagcatgta agcaatcaat cccatgctga tagtaaagaa gatcgtgttc aatactaaac
    35941 gcatctttac atttttcact ttgttttgca gcaaagctgt cagacctaaa gaaagcaatg
    36001 tttggccacc accaaaaacc acatccatga taccaaatcc aaagaaggca ttatagacga
    36061 tgaccccgcc caaaactccc caaagtaatt tacggttaaa gacaactaaa tgattcaaac
    36121 tttctgaaat acgaaactgg attggccctg aggatacagg cgcaactaaa atagttagcg
    36181 ctagataaag agccatgatg ataccattga caacgattac ggacaaccgt tgtctgcttt
    36241 tcgttttttg cattttctct cctcatttct cctagttttt ttaacgcggg atggttgatg
    36301 aaccgcgatt atgtgaaaca aacaataatt ataacagact tttcatctat ttcaatctgt
    36361 cttatctact tttgaaaaaa gtaaacaagc aaaaatgtaa acgtttgcta aaagtctcaa
    36421 aatatggtat aagataaatg taaggggtca taagaattcc cagtaaagca gacgaagtag
    36481 gttctgcacg aacagaattg ttgtgaaact taaataaata aaaaaggatg tgggaatttg
    36541 agcaacctct tactagagaa tagtctagca ggcagctgaa ggtctaagtg gacataccga
    36601 accttggaac ttctccttac gtaagtaatg gatgatgcgc ttgctaggct tttttgtgtg
    36661 tttcggtctc cagacctttt tcattctcct agtcttctgc tataataacc aagaattaat
    36721 tttgtaacag gtgttttttt gtaaaaacaa gtttctttca ttccaaaaat agaaaaaact
    36781 tgctaattat tggttaacca gcaaacagga tgattaaaat atcttgtttt attgaatagc
    36841 aggaggaaaa tatgaaattc cattatcatt tattgatcaa tccatcggca ggcagtggaa
    36901 acggccataa agtagcagaa aaaatcttgc cgatattaaa aaacaaacat gtcaactata
    36961 cccttcatta cagtgaatac aaaggacatg atgcggagat tgctgaaatt ctggcaaaag
    37021 aaacattagt accttggaca gaagaacagg aaacaatcga aactttccct cttttgatca
    37081 tcgtaggagg agacggaacc ttgcatcagg tacttgatac tttctatcag atggaagttg
    37141 aatttcctgt cgcttatatt ccagcaggct ctggaaatga ttttgctcga ggagcgggac
    37201 ttcctaagaa tcctaaaaaa gcacttcagc tgattttagc agctcaatca ccagataaag
    37261 tccatataat ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt
    37321 ttggtattgg attagacgca gctatcgtcc acgcgacaaa tcattcttct acaaaagaac
    37381 gattgaacaa atacaactta ggttcttttt cgtatttgtt ctctattctc cggactttat
    37441 ttactcaaaa aggatttcca attctagtgg atgcaggagg aaagcagtta agtttttcaa
    37501 atgcattttt atgtacagca acgaagcatc ctttttttgg aggaggaatc gcaatcgctc
    37561 caactgcaga tgcgactaaa ccagtcattg attttgtcat ggtcgaacga atcaatattt
    37621 ttaaaatcat atggcttatc cttttactta tgcagaaaaa acaattgaaa tcgaaatatt
    37681 tccatcacta cacaacaaac agattacgaa tcgtttcacc aacgccgcaa tatggtcaag
    37741 aagatggaga agatgtcgct cctcgtcctt ttgatctgca attttctaca aaaaaccagt
    37801 tatttttcaa gtagtttcga gtttgtagaa aaattttgtg gaaatgcctg ttcacatttt
    37861 tgcaaaaatt tgacttcata ccaagaaact gtcttttgaa caccgtttac caagacggca
    37921 tgagcctaca atttcactac aagtcaccaa gaataatttc aaaaagcgga tgaggcacaa
    37981 gtcgtgtcac atccgctttt taagtgatca atattgccga caaatagcgc atacgtatcg
    38041 tcagattctt ttataatgta tacggtgtaa tgttttccat gccaatcatt ggatctacta
    38101 atttctgatt aatcgcttct gcagtaagaa cgaactcctt gggaacagca ggttctatct
    38161 cttctttctt ttcttctaca accgatgaag aaaatagatc tggttccact gtttccatta
    38221 cttggttttt cgattcattc gttccactgt cgtcttttat aggtaacttt tcttttatgt
    38281 cctgcgactc ttttgtatag gtcccatctt caaatggatc atttccaatc aaagaatctt
    38341 cataagctgc tagttttgtc cgcagagtga tagtcatttt tccattatcg atgattcttg
    38401 atttcaacgt agtcattcgg cttgccgatt cattcgctac acaagtggca tacgcttgct
    38461 cttcccctaa caaaatcaac agttccttac tccgagtaac agccgtgtag agtagattac
    38521 gctgaagcat tcgctgatat tgatgaacca tgggcaaaat gaccattttg aattcactgc
    38581 cctgtgattt atggatcgaa caacaataag aaagagtaat tttattccac tcgtttcttt
    38641 tataagtgac ctcatttgca tcaaactgga taactaattc atccacttta tcttccgaat
    38701 cttttgccaa gataatcccc gtgatttctc ccatatctcc attgaataca ttcaattcag
    38761 gagtattgac taactggagt actttgtctc ctatccgata aacggtgtcg ttccaatgta
    38821 cttccttttt cccaggtctc cctggattga atatctcttg catcatttta tttaacgcgt
    38881 cgatccctgc tggtccccga tacattggtg ctaatacttg gatatcttgt gcacggaatc
    38941 ccttattttt cgctttttcc acaattttag aaatcaaggg ttcaatttga taagcatcgc
    39001 ttcggaaata ggaacgatct ttttgattct ttgtgaaatc ttttggtaga cgtccttctt
    39061 tgatttcatg ggcaagagga ataatactag aaccatcacc ttgtcgataa atctgattga
    39121 gttccatttt aggaatttgt tcgatttcca aaaggtcatg tagcacttgt ccaggaccga
    39181 cagaaggaag ctgatcttta tcccctacta gaataacctg catattcgtc ggaatcgctt
    39241 tgaataatgt gttagccagc catgtatcga ccatagacat ctcatcaacg atcaaaagtc
    39301 cgccttctaa ttctttcgtg ctgactgaag gtgttttttc ccgtccattc agcccaagta
    39361 aacgatggat tgtactggca ggcagacctg ttgtctcgtt cattcgttta gctgcccggc
    39421 cagtaggtgc tgcaagcaga ataggaaaaa tttcttgggt atattttgac ggatccaatt
    39481 ccagaccatt caactctgca aaaagattga cgatcccatt gatcacagta gttttccccg
    39541 tacctggtcc tcctgttaaa ataaatagag gagaacggat ggcttcagcg attgcttgtt
    39601 cttgcgaagg tccatactgt ataccaaata ttttttcgat acgccgaata tttttctgca
    39661 cttctttttc aggataatgg atttcttttt tacggctgag cagacgttgg atggaattgc
    39721 cgatacccca ttcagaaaaa tacaaactgt tttcgtaaag attcgtttct tcttgctgga
    39781 tttttccttc ttcaacaagc tggataatca cagtagcaac ctgatcaggc agaatttcta
    39841 ttggacgact tgtttctaaa atacggatgg tttgctgaag caatgtttcc gccggtacgt
    39901 atgtatctcc tgtttgaaca gcatgctgaa aaatctgatg caagatggct gctcggattc
    39961 ttctaggtga atctgctgca ataccgattt gttcggctag attatctgcg cgtttgaacc
    40021 cgattccttc tatgtcttcg actagctgat acggattttc ttcgatgata tccaaagact
    40081 cgttcttata ggtctgataa atactgaaag ctaactgact accgaatcca taacgattca
    40141 atcccatgat tacttgatcc atcccgtgat ttagacgaat cgtttcagca agcatttctc
    40201 tttttggctt cgttaaacca gcgatttgtt ttaataccga aggatcagca atgatttttt
    40261 cgatagcatc ttctcccagc aactcgacga ctttctctgc tgttttctta ccgatcccag
    40321 ggaatttttc gctggaaaga taagctacga tcccattcgc agttgttggt ttttcttgct
    40381 catatctttc gactttaagc tgtcttccgt atttagggtg atccactagt tctccataaa
    40441 aacgataggt ctcattttct tgaatatcgc caaagcttcc ggtaactaca atctcttttt
    40501 cacggtaatc agctgtagtt tcgctgatgt gtaccaaaag cactttgtaa aaattactag
    40561 gattttgaaa aaaaatcgcg gcaactgtac caacgaaata aggcatttct tctggctgca
    40621 tcggaagccg cctccttctt ctatagtaaa ggttctggtg ctgtttcagt tcctaaaaaa
    40681 tctgtttggt tccatacttt cagtttatac ggcaataatt tgcaaggctc ttcagtctct
    40741 aaaatcgtca ggctgctatt gaacaaaccg cccatttccc gcaactcact tagctctttc
    40801 cctgtcatcc attgaatggc agcagttaat gaggccccat gacctacgaa taaaagaggt
    40861 ccgtttttat gttgagcaac cgcatccgct acaacagttt caatacgtgt gattgctgat
    40921 tcaatcgttt ctccatgaaa aggagaagga tcatatagat ctaagtgatt acgcaaattc
    40981 ggcaaattct ctgggtattt ctgatacatt tcatcgattg attgtccttc taaagtacct
    41041 agccctagtt ctcgcagttt atctgtatag aaaatctcta ccggactagc aagctctttg
    41101 ttgatacctt ctgctgtatc gcatgctcgt tttgctgtac tggaataaat tgctttaaaa
    41161 ggaacatatt ttactttttt cccaaatgct ttgatttctt cgtaactttg aggcaaaagc
    41221 ggagaatcgc cattgctccc ttgaaaacgt ctttcctgat tccattgtgt cttcccatgt
    41281 cgggtaaaat atagttccat acgcagcaaa ctcctgtctt tcttttattt tactctataa
    41341 taaactgtct aatccagctt gtcattttca taatggtgcg ttaattctaa attagggaat
    41401 ccttgctgac gaagtgcttc gtacacaatc attgcagctg tattcgataa attcaatgaa
    41461 cgaacatgct catcattcat agggatgcgc aaacactttt cctcgttttc gcgcataaac
    41521 gcttctggca agccagttgt ttcctttcca aataaaaaga agtgctcttc tccatcagca
    41581 aaattttctt cactgtatac cttatgagca aattttgaga tcaagtgtag tttacgatca
    41641 cctaaaaatg ttaaaaaagc ttctaaatca ctgtgataag taatatttac atcgttccaa
    41701 taatctaacc ctgctcgttt caggtgtttg tcatccgttg aaaaacctag gggctcaatt
    41761 aaatgaagcg gcgtattcgt ggccgcacac gtacgggcaa tatttccggt gtttgcaggt
    41821 attaacggtt cgaataaaac gatatgattc atcaggatca ttcctctcat atttatgttc
    41881 gtttctattt tctgacaatt tgatttttgc ataaaaaaaa acgtatcaac tcggtgtcag
    41941 tcactgcgag tcgttacgtt agtgaagctc ttctcactaa cttttatcag aacaggaacc
    42001 tgataaaaac caatgaaaaa acaacttgtt gctactataa caccctcaac ttacttttgc
    42061 aacctctact ctttaaaaac tactagaaaa ccgttttctc ttagttcctt ttaataattc
    42121 tatgtcaatt gtaatctttt tcaaaggatt cttgtacaag tctgctttga tttcctcctc
    42181 gacttgccac tctaagggcg acattgctaa aagatctttt cgattttctt ctggtatctc
    42241 aaattcataa gtcacacgct gacgttcagt tagtttcatc gattcagtaa actttgcgac
    42301 tacacgatca ttagaataac tttgcttttc aggttgattc ggataaaaag cctgacgcag
    42361 ttcttttaaa taattttctt gcggcactac cttcagcacc tctccatctt ctgacagtac
    42421 acgttggaat tcttggtaat gagaaggcga aaagatattt aagatcgtgt taaatgtctg
    42481 atcagcaaat ggcaaattgg tcaaatccgc tacacaccaa aatgcctcga cagattgttc
    42541 cgtagctgca tagacccctt cttttgaaat atcaaatcca acagcagttt gaatcttttt
    42601 caattttgaa agttcattca agaagcttcc ttctccacag ccgacatcta atagcgagat
    42661 ttcattaggc agatactgtg cgatagtctc aataacgggc tgatacatgc ctgattggat
    42721 cattctacgc cttggaacga acatttcttg atcatattct gttttaaccg cgtgggataa
    42781 aaagtaaagt gttccttttt tggacagatc aaaacgatga ttatttttgc atatcaagcc
    42841 attatcagac ttttctacct cttcttggca aactggacag cggaacagct gatgtttttc
    42901 tgccaaaaac tggcgtcctc ggtcaatttt ttttaacata gttccccctc atttctgttt
    42961 tatcttaccg ttagatgctc actttggcaa ctggtaaaaa tatgatacaa tgcctagaag
    43021 aaagcgaaag acaaaggaga agatcagatg atcaaagaat tttgtgctga aaactacaca
    43081 tctattcctc tggcaattgc cagaggagct aaccgaatcg aattatgtga caacttagcg
    43141 gtcggcggaa cgaccccaag tactggtgtg atcgaagaaa cattaagtta cgcaggggaa
    43201 aaaaatatcc ctgtaatgac gattatccgt cctagagggg gcgattttgt ctataacgat
    43261 atcgaattga aaattatgca tactgatttg attgaagcaa agaaactagg gacggatggc
    43321 gtagtttttg gttgtcttac tccctcagga tggctagacg aagaagcatt ggaactattg
    43381 atcgaaaccg cagaaggcgt acagatcacc ttccatatgg cttttgacag tattcctgaa
    43441 gaacggcaat ttgaagcaat cgattggcta gcagcgcacg gtgttgaccg tatcttaacg
    43501 catggcggcc ctgctgatca aacgattgaa gaacatttcc cacggctcaa agaattgatt
    43561 gactatgcag atggccgttt gatcattttg cccggtggtg gcgtaacagc agcgaatgca
    43621 gcacatgtag ctaaagaatt aaacgtgagt gaagtccacg gaactaaaat cgttgaactt
    43681 cagccataat cttataaaca aaaaacattg gcaacaagta tactgcttgt tgccaatgtt
    43741 tttgcttttt acataaaata ccccataaat aaagaggcaa tattgaaata aatcagtaca
    43801 cttgtcaaat cactcaaggt cgtgataaat ggaccgcttg ctaccgctgg atcaaatcct
    43861 agacgatcca tcagcatcgg aatcaaacta cctgcaagat tcgccactgt tatcgcaaac
    43921 aacatggcca tccctatcac aaatcctaag ataaaattat gtttccaaaa gcccacaatc
    43981 agaaaaatcg tcatccctgt aacagctcct gttacgacgc cggtcaagac ttcagccaaa
    44041 acaagtcgga caaacccgtg atctttgtca tcagagattg caagtcgacg gacagccaca
    44101 gccagcgact gggttccagc attaccagct gttcctgtga tcagcgaaat gaagaccgct
    44161 aaaatgctcg cttcacttac caagtcttca taatgactga tcaaagaagc agtagacata
    44221 cctaaaaaca acagagtgat tagccaaggc aaacgtcgag atgctgcctt tactggattt
    44281 tcattgattt cttccacatt gaccccggca agtcctgaat aatcgctggc agcttcatca
    44341 tcaataacgt caatgatgtc atcaactgtg acgattccca acaaatgatc atcataatcg
    44401 gtaacaggaa gagctaagaa gtcataatcc ctgaatgttt gtgccacatc ttcttggtcg
    44461 tcgcctacat gaactgaaag cacccgctcg ctcatcagat cagaaatcat agcatcatca
    44521 tcattcacga ttaggtcgcg tagagaaata acccccacta gttgaccttc ttgattaacg
    44581 acatagatgt aataacccgt ggtgctagtt aattttaata tcctaaagaa tatctcaaag
    44641 ttccttcaaa acgtcgttgt gctcgactta atagaacact gtaagccagt aatatgct
//
LOCUS       DADJYG010000009        44514 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.009, whole genome shotgun sequence.
ACCESSION   DADJYG010000009 DADJYG010000000
VERSION     DADJYG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.009"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..2634
                     /gene="adhE"
                     /locus_tag="JZ382_000406"
                     /gene_synonym="adhC"
     CDS             37..2634
                     /gene="adhE"
                     /locus_tag="JZ382_000406"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY2426617.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2863..3969
                     /locus_tag="JZ382_000407"
     CDS             2863..3969
                     /locus_tag="JZ382_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAY2426618.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3986..5014
                     /locus_tag="JZ382_000408"
     CDS             3986..5014
                     /locus_tag="JZ382_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAY2426619.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5020..5499
                     /locus_tag="JZ382_000409"
     CDS             5020..5499
                     /locus_tag="JZ382_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY2426620.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5515..6810
                     /locus_tag="JZ382_000410"
     CDS             5515..6810
                     /locus_tag="JZ382_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAY2426621.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6821..7645
                     /locus_tag="JZ382_000411"
     CDS             6821..7645
                     /locus_tag="JZ382_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY2426622.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7810..8709
                     /locus_tag="JZ382_000412"
     CDS             7810..8709
                     /locus_tag="JZ382_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY2426623.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8835..9704)
                     /locus_tag="JZ382_000413"
     CDS             complement(8835..9704)
                     /locus_tag="JZ382_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY2426624.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9857..11317
                     /gene="rhaB"
                     /locus_tag="JZ382_000414"
     CDS             9857..11317
                     /gene="rhaB"
                     /locus_tag="JZ382_000414"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY2426625.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11314..12570
                     /gene="rhaA"
                     /locus_tag="JZ382_000415"
     CDS             11314..12570
                     /gene="rhaA"
                     /locus_tag="JZ382_000415"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY2426626.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLEEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12584..13429
                     /gene="rhaD"
                     /locus_tag="JZ382_000416"
     CDS             12584..13429
                     /gene="rhaD"
                     /locus_tag="JZ382_000416"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY2426627.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13431..13742
                     /gene="rhaM"
                     /locus_tag="JZ382_000417"
     CDS             13431..13742
                     /gene="rhaM"
                     /locus_tag="JZ382_000417"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY2426628.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13824..14975
                     /gene="fucO"
                     /locus_tag="JZ382_000418"
     CDS             13824..14975
                     /gene="fucO"
                     /locus_tag="JZ382_000418"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY2426629.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15102..15860
                     /locus_tag="JZ382_000419"
     CDS             15102..15860
                     /locus_tag="JZ382_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAY2426630.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            15960..16604
                     /locus_tag="JZ382_000420"
     CDS             15960..16604
                     /locus_tag="JZ382_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY2426631.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16611..17618
                     /locus_tag="JZ382_000421"
     CDS             16611..17618
                     /locus_tag="JZ382_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY2426632.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17634..18476
                     /gene="kduI"
                     /locus_tag="JZ382_000422"
     CDS             17634..18476
                     /gene="kduI"
                     /locus_tag="JZ382_000422"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY2426633.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18496..19299
                     /locus_tag="JZ382_000423"
     CDS             18496..19299
                     /locus_tag="JZ382_000423"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAY2426634.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19548..20612)
                     /locus_tag="JZ382_000424"
     CDS             complement(19548..20612)
                     /locus_tag="JZ382_000424"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY2426635.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20609..21694)
                     /locus_tag="JZ382_000425"
     CDS             complement(20609..21694)
                     /locus_tag="JZ382_000425"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAY2426636.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="JZ382_000426"
     CDS             complement(21707..22684)
                     /gene="mvaD"
                     /locus_tag="JZ382_000426"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAY2426637.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="JZ382_000427"
     CDS             complement(22677..23621)
                     /gene="mvk"
                     /locus_tag="JZ382_000427"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAY2426638.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23943..24596
                     /locus_tag="JZ382_000428"
     CDS             23943..24596
                     /locus_tag="JZ382_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAY2426639.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            24681..25586
                     /locus_tag="JZ382_000429"
     CDS             24681..25586
                     /locus_tag="JZ382_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAY2426640.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25588..26220)
                     /locus_tag="JZ382_000430"
     CDS             complement(25588..26220)
                     /locus_tag="JZ382_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY2426641.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            26540..27064
                     /gene="infC"
                     /locus_tag="JZ382_000431"
     CDS             26540..27064
                     /gene="infC"
                     /locus_tag="JZ382_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY2426642.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27136..27336
                     /gene="rpmI"
                     /locus_tag="JZ382_000432"
     CDS             27136..27336
                     /gene="rpmI"
                     /locus_tag="JZ382_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY2426643.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27389..27748
                     /gene="rplT"
                     /locus_tag="JZ382_000433"
     CDS             27389..27748
                     /gene="rplT"
                     /locus_tag="JZ382_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY2426644.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28000..29337
                     /locus_tag="JZ382_000434"
     CDS             28000..29337
                     /locus_tag="JZ382_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAY2426645.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29385..31514
                     /locus_tag="JZ382_000435"
     CDS             29385..31514
                     /locus_tag="JZ382_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAY2426646.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31489..32178
                     /locus_tag="JZ382_000436"
     CDS             31489..32178
                     /locus_tag="JZ382_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426647.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32586..33272)
                     /locus_tag="JZ382_000437"
     CDS             complement(32586..33272)
                     /locus_tag="JZ382_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2426648.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIRRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(33408..33602)
                     /locus_tag="JZ382_000438"
     CDS             complement(33408..33602)
                     /locus_tag="JZ382_000438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426649.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33652..34092)
                     /locus_tag="JZ382_000439"
     CDS             complement(33652..34092)
                     /locus_tag="JZ382_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAY2426650.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34096..34941)
                     /locus_tag="JZ382_000440"
     CDS             complement(34096..34941)
                     /locus_tag="JZ382_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAY2426651.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35090..36508
                     /gene="pepV"
                     /locus_tag="JZ382_000441"
     CDS             35090..36508
                     /gene="pepV"
                     /locus_tag="JZ382_000441"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAY2426652.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36565..37512)
                     /locus_tag="JZ382_000442"
     CDS             complement(36565..37512)
                     /locus_tag="JZ382_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY2426653.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37735..38085)
                     /locus_tag="JZ382_000443"
     CDS             complement(37735..38085)
                     /locus_tag="JZ382_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY2426654.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38285..39364
                     /gene="pepA"
                     /locus_tag="JZ382_000444"
     CDS             38285..39364
                     /gene="pepA"
                     /locus_tag="JZ382_000444"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAY2426655.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39508..39828
                     /locus_tag="JZ382_000445"
     CDS             39508..39828
                     /locus_tag="JZ382_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAY2426656.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39850..40314
                     /locus_tag="JZ382_000446"
     CDS             39850..40314
                     /locus_tag="JZ382_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAY2426657.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40519..41124
                     /locus_tag="JZ382_000447"
     CDS             40519..41124
                     /locus_tag="JZ382_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAY2426658.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41163..42452)
                     /locus_tag="JZ382_000448"
     CDS             complement(41163..42452)
                     /locus_tag="JZ382_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAY2426659.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42880..43359
                     /gene="rlmH"
                     /locus_tag="JZ382_000449"
     CDS             42880..43359
                     /gene="rlmH"
                     /locus_tag="JZ382_000449"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY2426660.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43444..43953
                     /locus_tag="JZ382_000450"
     CDS             43444..43953
                     /locus_tag="JZ382_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY2426661.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44053..>44514
                     /locus_tag="JZ382_000451"
     CDS             44053..>44514
                     /locus_tag="JZ382_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426662.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTEN"
BASE COUNT        14118 a         8074 c         9549 g        12773 t
ORIGIN      
        1 tttaacaaat aaccatgacc cattaggagg ataatgatgg ctaaagtaat agaaaaagaa
       61 aaaacgaaga caattgacgt acaagcaatg attgatgatt tggcagaaaa agcaaatgca
      121 gcattgaaag aaatggaaag ctttgatcaa gaaaaagtag accatatcgt gcatgaaatg
      181 gcaatggcag ctttggatca acatatgcca ttagcgaaga tggctgtaga ggaaacaggc
      241 cgcggtgttt acgaagataa agcaatcaaa aatatgtatg cgtccgaata tatttggcat
      301 aatatcaaac atgataaaac agttggtgtg atcaatgaag atgtccaaaa agggttgatt
      361 gaaattgctg aaccagtcgg tgtggtttgc ggagtaactc caacaacgaa tccgacttcc
      421 acaacgattt tcaaatcaat gatcgcgttg aaaacaagaa atccaatcgt ttttgctttc
      481 catccaagtg cccaaaaatc ttctgcagaa gcagcacgag tggtacgtga tgctgctatc
      541 gctgccggag ctcctgaaaa ttgtatccaa tggatcgaac atccttcgat cgaagcgact
      601 tctatgctaa tgaaccatcc aggtatcgcg atcgtcttag cgacaggtgg agctggaatg
      661 gtcaagtctg cttactctac aggaaaacca gctttaggtg taggtccagg taacgtacca
      721 gcttatattg aaaaaacagc aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa
      781 acatttgata atggaatgat ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc
      841 tatgcagcag taaaagcaga attccaagca caccaagttt atatcgtaaa accagatgaa
      901 ttgcaaaaat tagaagatgc agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc
      961 gttggacatt ctgcgatgga aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg
     1021 aaaatgctga ttgctgaatt ggaaggcgta ggcccagatt atccgctatc aagagaaaaa
     1081 ctttctccag ttcttgccat gatcaaagct aacaacacgg accatgcgtt tgatttgtgt
     1141 gaaggtatgt tgaatcttgg aggattagga catacagcag ttatccattc agaaaacgaa
     1201 gagttgcatg tcaaattcgg tcttcgaatg aaagcttgtc gtattttagt gaatacacca
     1261 tctgcagaag gcggcatcgg tgatatctat aacgaaatga ttccgtcatt gactttggga
     1321 tgcggttctt atggtaagaa ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat
     1381 gtcaaaactg tagcgaaacg gagaaataac atgcaatggt tcaaattacc gccaaaaatc
     1441 ttctttgaaa agaactctct attgtactta gagaaaatgg aaaacgttga acgtgtcatg
     1501 atcgtttgtg accctggtat ggtccaattc ggttactgcg atacagttcg tgaagtattg
     1561 gcacgacgta aaaatgatgt gaagatcgaa gtcttctcag atgttgaacc gaacccatcg
     1621 acaaataccg tatacgcagg aacgaaactg atggcagact tcaagccaga tacagtaatc
     1681 gctcttggag gcggttctgc aatggacgca gctaagggaa tgtggatgtt ctatgaacac
     1741 ccagacacat ctttctttgg tgcaaaacaa aaattcttgg atatccgtaa acggacatat
     1801 aagatcgaca aaccagaaaa aacacaattc gtatgtatcc cgacaacatc agggactggt
     1861 tctgaagtaa cgccatttgc ggtcatcact gacagtgaaa cacatgtgaa atatccgtta
     1921 gcagattatg cattgacgcc agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt
     1981 ccagcatctg tcacagcaga taccgggatg gatgtgttaa cacatgcaat cgaatcctat
     2041 gtttcagtca tggcttcaga ttatacacgt ggattgagcc tacaagcaat caagttagtc
     2101 tttgactatt tagaaaaatc agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac
     2161 aacgcttcta caatggctgg tatggcgttt gctaacgcat tcctaggtat ttgtcactca
     2221 gtcgcacata aaatcggcgg ggaatacggg atccctcatg gacggacaaa tgcgattctt
     2281 ttaccgcata ttatccgcta taatgcaaaa gatccacaaa aacatgcgat gttccctaaa
     2341 tatgactatt tccgtgcaga tacagattac gcagatatcg caaaattctt aggtctgaaa
     2401 ggcgaaacaa cagaggaatt agtcgaagct ttagcaacgg ctgtttatga attaggaaaa
     2461 tctgtcggca tcaacatgag tctaaaagct caaggagtca cacaagaaac actagataca
     2521 accgtagacc gtatggctga attagcttac gaagaccaat gtacgacagc taatccgaaa
     2581 gaaccattga tctctgaatt gaaacagatc atcattgatg cttacaacgg ataagcaaaa
     2641 gaaagcgtag aaaaaagata agaaaataga tgaaaaagaa cttggaaaac gttgtttctc
     2701 caagttcttt ttttaatttc caagtaactg aaagttactt ttgttattta acatttaggt
     2761 caattttaga cagtttttgt tcaaaaagcg atatttatag tggacggtat tactgctata
     2821 ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa agatgattat taaagaagag
     2881 atttttgtac ctaaagaatt agtgaaagaa aaaattgacc tgctcgttga aaacctgaca
     2941 acgatcaaag acgataatgg tgaatttttg ttggactttg atggactgaa agtagatgac
     3001 aaaagctgga cagtgtggaa ttggccacaa ggtgtcggtc tatatggtat ttataaaaat
     3061 taccagatga caaaaaatcc tcgcgcttat caagtggtca atgaatggtt tgaggatcgt
     3121 atggaagaag gcgctccgcc caaaaacgtc aatacaatgg caccgctatt gactatggct
     3181 tacttgtatg aagacacgaa agattcacgc tatttgcctt atttagaaca atgggcagaa
     3241 tgggtcatga atgacatgcc tcggacaaat gaagacgggt tacagcatgc cacatatgga
     3301 ccggaaaata agaatcagtt gtgggacgat acattgatga tgacagtgct tcctttggca
     3361 aaaatcggga aactgttgaa tcgcccagaa tatttagaag aagctcgtta ccagtttatg
     3421 atccatatca aatacttgat ggataaacga acaggattgt ggtatcacgg ctggacgttt
     3481 gaaggaaatc ataactatgc cgaagcattt tgggctcgtg gaaactgctg gttgacgatt
     3541 gctattccag agatcattga aatcttagaa ttatcgaaag aagatgcatt gcgcaaatta
     3601 ttgatcgaaa cacttgaagc acaagtgcgt gcattaaaaa cttatcagtc tgaaagcggt
     3661 ttatggcata cgttgttgga tgatccttct tcttacgtag aatcttctgc aactgcagga
     3721 tttgcttacg gtattttgaa agcggtccat aaacgttatt taccacaaga atataaagaa
     3781 gtcgcttatc gtgctatcca aggattgctt gaacaaatcg atgaaaaagg agaagttcaa
     3841 aacgtttcaa tcgggacagg aatgggggac agtttagatt tttatcggaa tatcggaatc
     3901 acagcaatgc cttatggaca gtcgttaacg gtgctttgtt tgacagaatt gctcgtctct
     3961 tattgttaaa agaaaggaaa cccatatgaa aaaaacatca ctgctgtttg cagcaagttc
     4021 gcttagccta ggtctgctag gtctaagcgg ctgcggaaat aattctagtg tggcaactgt
     4081 cgcagatgca gatgtaaatg agatagttac cttgcgcttt gcttatgcaa gcaacagcca
     4141 gccagtaatt gatgcaatga atgaatttgg gcgtttagta aaagaaaaaa cagatggttc
     4201 cgtacagatc cagtatttcc cagatggaca actgggtgga gaacgtgaac tgatcgaatt
     4261 gacacagtca ggagcagtag atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc
     4321 taaagattat tcgatttttt cagttcctta tctattcaat gatgaagaac atttctttaa
     4381 ggtgatggat aatgaagaaa taatggagcc aatctaccaa tcgaccaaaa atttaggatt
     4441 taccggattg acctattatg attctggtca gcgcagcttt tatatggtag acggtccgat
     4501 ccatacgccg gaagatttga agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat
     4561 caaaatggta gagcttttag gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac
     4621 gtctcttcaa tccaatttga tcaacggatc agaaaacaat gaatttgtct tgtatacagc
     4681 tggacatggt ggtgtggcta aatactattc ctatgatgag catacacgtg tgccagatat
     4741 catcatcatg aatgatgcca tcaaagaaca gctaactgat gagcaggaaa aagccattga
     4801 agaagcagcg aaagaatcaa cggtttttga aattgaagct tttgcaaaag caattgaaga
     4861 agaaaaagct ttagcacaag aagaacatgg agtccaattc aatgatgtag ataatacacc
     4921 gtttcgagag gcagttgccc cacttcatga tatattcaaa aacgatccta cctacaaaga
     4981 cctttatcaa aagatccaag agcaaggagc gtagctgtga tgaaaatgat tttaaacaag
     5041 gtattagaga tcctgggagc tgttttgctt gttgggatgg tcgctgttgt tttatggcaa
     5101 gtattttcca gaacggtttt aggaaatcca aatacggtca cggaagagct tgttcgattc
     5161 ggtcttgtct ggtttgccat gttgtcttcc gcgtatgttg tgggacaaaa agcacattta
     5221 gccgtaacgg ttttaagtga aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt
     5281 gttcaaagtt tatttctggt atttgctgcg atgatcatgg tgtatggcgg atggaatgct
     5341 gtcacattga caatggggca gatctcgcct tctttaagta ttcctatggg atatgtttat
     5401 ctgtcagtac ctgtatcggg ggtactcatc attatctata gcctgatcaa tctaatcgat
     5461 acattaagca acaagccagc aattcaagaa aaaatataaa aatggaggaa taaaatggca
     5521 atagaagccg gattagtgtt attttttgta tttgttgtgt tgcttgtatt agggatgccg
     5581 attgctataa gtattgcatt ctcatcgatg atgactttat tgttagttat accgtttgat
     5641 gtttctgctt ttagttcagc tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc
     5701 gctattccgt ttttcgttct ctcgggcatt atcatgaata atggagggat agctaagaaa
     5761 ctagtggatt ttgcgatgtt gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat
     5821 gtgttaggga atgctttatt tggttccatc tctagctcgg ccattgccgc ttctaccgcg
     5881 attggcggag tgttgatccc tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca
     5941 gcggtcaata tcgcgtcagc tccgacaggt atggtcattc ctccaagtac tgcttttatc
     6001 atgtattcac ttgttgctgg tggtgcttcg atctctgctt tgtttatggg aggttatctc
     6061 gtaggcgctt tgtgggcgct aggaatcatg ctagtagcat tcatcgttgc taaaaagaat
     6121 aaataccctg ttgtcgaaaa aggacagatg gctcatgctg gaaaaataat aaaagaggcg
     6181 attcctagcc ttctattgat tgtcatcatt atcggtggta tcttgacggg gatatttaca
     6241 gcagtagaag cttcagcgat agctgtcatt tactccctgt tgattgctat gttttactat
     6301 aaaacggtaa aactaaaaga tatacctaat atgttgtttc aatcagtagc aatgtcaggg
     6361 acgatcatgt tcttattggc tacttcttcc atgatgtctt tcgccatggc gtttaccggt
     6421 attcctcagg caattagttc gttgattatg ggcgtaacgg ataataaatt tattattctg
     6481 ttattagtca atcttgtgtt attactggtg ggtatgttta tggatgtggc gcctgctatc
     6541 ttgattttta caccaatatt tttaccaatc gtcacaagtg taggggttga tcctgtacat
     6601 tacgggttgt tttcaatcat gaatctgtgt gtaggctcca tcacgccgcc agtaggtaca
     6661 ggactatatg tcggtgcaag tgtaggaggc gtaaaagcag aggcaatgct gaaaccgctt
     6721 cttccttttt atggggttat cttagtcgtc ttgtttttga ttacttactt cccgcaaatc
     6781 gtcatgtggc tacccaatat gctgagttag gagaatagtt atgttgaaca ttacaattag
     6841 aggacatgat ctttccgaag tccaaacgat agaagatctt gcagaaaaaa caaaagaaca
     6901 agggattcat acactgcagc tcgccttagg tctttcgttt cctgaaatac cttctggcgc
     6961 aaatgagatc aattctggga tgggaaatta tgtcaaacga gttttagaaa agcaagaagt
     7021 gaccgtagga attttaagtt gctatatcaa tatgattcat ccagatttaa cgatacgaca
     7081 agagttgctg acaaagttcg agcagtatct tcgctatgct tcttcctttg gtgcgtcgat
     7141 ggttgcttcg gaaactggat gtgttttgcc ggaaatccaa tatacggaag aaaatttcac
     7201 agatgaagca tttgctgaag ctgtgtccgt tatccgtcgc ttggtaaaag ctggagaaaa
     7261 ataccaaatg atggtgggga tagaaccagg tttgaatcat ccggtgtatt ctttagcaag
     7321 ggtagagcag ctgatccaag cagtagattc ggactatcta ggaatcattt tagatccgac
     7381 caatttgatc acaagtagtg attatcaaga acaagtccaa ttggtagaag aagcttttga
     7441 acgttttggt gaaaagatat gtgcggttca tttgaaagat tttcgtgtcg aacaggaaaa
     7501 aatcgtgccg gtaaatttgg gagacggtgt gatcgagtac acaaaaatca aagaaatcat
     7561 catgaagaat cgtccgtacc tgtatgtggt tcttgaagaa acaaaagatg atgggattcg
     7621 ttatggaaga agtcttcttg aataatgtaa agaagacgtt ggttaactaa tatttagaat
     7681 ggagggggcg gggctagaag atgccacgtc tcttcttttt tataagagta tataaaagca
     7741 gctctttttg attacaaaaa acgtggtata ataaaattgt gcataagtga acaaaaggag
     7801 gtgcggcaca tgctccaaaa gaaaatcagc ggtgaacaag tcaatacgat ggtttctgat
     7861 cagtatgaaa tctatcatgt caaagatgtc gtcagccaaa ataaaacgat ctatcatcat
     7921 catgatttct atgagatcca tgctacatta aagggagaag cgacttttta tttgaacgga
     7981 cgacaattta cgatcaaagc aggaagtgtg ctgctcatcc attccaatga tttgcatcgt
     8041 atcgtccgcc aatcttcaga tgtattcgag cgtgtctata tctttgtcac cccttctttt
     8101 ctagaaagtc gttccacgcc atggtctaat ctatcggctt gttttcaacc aatgggagag
     8161 aagaggagtc gtgtattgca aatagaacag gaagtattga agcaaaaatt atcatttatc
     8221 gaccatcctc ctgataaaaa aatttacggt gaagacataa aatatgaaca agctctggta
     8281 gaatacttga tttttctcaa tcaagttgta caaaaagaag agagtgcgca agaacagaat
     8341 gctgtagttc ctaatgagcg tgtggaacaa atgttacaat atatctcaaa aaatttatct
     8401 catccgcttt ctttagagga aatggaaaag cagttttttg tcagcaaata ccatatcaca
     8461 cgagaattta aaaagtacac tggatttacg tttcatcagt atgtaatgaa gaaaaagctg
     8521 atctatgcca agcacttgtt gcgagaatac cgcagctcta gtgctgtata tagtaaaagt
     8581 ggtttttctt cttatccgca ttttctgaaa gcattcaaga gagagttcgg cataacgcct
     8641 aaagaatttt tgaaacgtga tacacagaaa cagttggttc attatgaaca ttttgaagat
     8701 gaacaatgag aatgatcact aaaaaataac cgcagtagct gctgttgttt acatagaagc
     8761 agaaactgcg gttattttat ttttatctat atatttcaaa atattttgaa aaaatgacgt
     8821 acatattata tgaatcagct gaatttacga aagtctctcg gcgatagtcc tgtttttgct
     8881 ttgaacaggc ggctaaagta agcaaaattc tcaatcccta tttcttcgga aatcattcga
     8941 accgtaaatt ctgtataccg aagaaggtct ttagcgtaag aaagtttttt gctgatcaaa
     9001 taatcgattg gtggagaacc gataaatttg gaaaaatctt tgctcagctg atatttattc
     9061 aataagaatt gttgttctaa ttgatcaagt gtaatcgttt ctttgaaatg ttcgtctaag
     9121 tattgcttca gtttgtaaac gtaggcggga atatcctctt cttcgaaatc tagctgatac
     9181 ttttgcaaaa tcagttcatt cagcaactcg tggatagcta cagaggactg gaaatcggta
     9241 cgggcattcg tgccttgttg catgtggatc aattctgtga tcaaatggtg gatcttgttt
     9301 tctgatggat ctcctgttga atggaaggtg ttatggccgt cttttgtgaa ctcctgataa
     9361 aatgattgga cgttctgacc attcaaatgg atccaatcca tttcccatgg ttcttcgctg
     9421 atcgtttgat aacgctgata ttcttggcag tcaataaaaa ataagtctcc agcttttagc
     9481 tgttgctgat tatcattata tgtaagcgaa cctttgccag acaaagtaaa ctttatcaag
     9541 taagaaggta aatgtgctcg ttcagtatag taagggattg gtgctttgaa gtaaccgatt
     9601 tcttggatat aaaaaaataa attcctgctt ctttgtgtag gcgtcaagat cactcttttt
     9661 gagtcttccg accatgagtg gttggctttt tcccagtaat ccatgtaaac actcccaact
     9721 tgtcaagata atgcaattat ataccaaaat agtgtgaagc aaaatataag attctgtatt
     9781 atgatagcat tgtcaataaa tgataacgct ttattaataa agtgctaatc aacaataaag
     9841 taaaagaggt ggagaaatga atccaacatt tgttgccatc gatatcggtg catcaagcgg
     9901 acgactaatg aaaagtacga gggatgcaaa aggttctttg atcttggaag aagtacaccg
     9961 gttcaaaaac ggctttcgga acaaagacgg tcacgatcgc tggaagatcg attatcttat
    10021 caaagagatt ttgattggat ttgaaaaatt aaagcagaca gggattgatc gcctttttgc
    10081 ggggatcgat acatgggcag tcgattactg tctgattgat gaacaagggg atcggataaa
    10141 tgatccgatt gcttatcggg acaagcgtac caatggagca gtagaacagt tttctgaaat
    10201 ctattctcta gataagttgt atgagcagac agggattcag atccagcctt tcaatacctt
    10261 gttccaattg tttgctgaag aaaaagaaaa tttggcaaaa gcacataaat tgttactgat
    10321 acctgattat ttagggtatg tctttactgg taacatggtg acggagaaaa ctaatgcatc
    10381 tactatgcag ctgctgaatg cagggacaaa agaatgggaa atagaacttc ttgaaaaaat
    10441 cggtgtctcg aaggaattat ttccaccatt gacagagccg ggaacgatac taggagatct
    10501 gcaaaaagaa caattttcaa aatatgattt gccagatgtg acctttatca cagtagcaag
    10561 tcatgatacg gcgtcagctg tattaggaac gccaggtcaa ggagaacgtt ggggttatat
    10621 cagcagtggg acatggtcgc ttttagggat tgaaacaaag atcgccaatg tttctcgcaa
    10681 tgctttttct gaaaattata cgaatgaatg gggtgcccat aacacggtgc gttttttgaa
    10741 aaacatcatg gggatgtggt tgatccagga agttgcccga atgcaagagt acgagtacag
    10801 ttatgcggaa ttggcagagc ttgctgcaaa agaaaaaccg ctgcagcaag tgatggaaat
    10861 caatgaccct cgatttttga atcccccaaa tatgatagaa gagattcagc aatactgttt
    10921 tgaaacagag cagccagtcc ctgtctcatc gggagctatc gctcgttgtg tgtatgataa
    10981 tcttgctctt tgttacgcac aagagctgat caaagtagaa cacttaactg gaacgaaatt
    11041 ggaaaagctg cacatcgtag ggggcggatc gaataatcgt ctactgaatc aattgacagc
    11101 agattatgcc ggaatcattg tagaagcagg acctagtgaa gccacagcaa tcgggaatgt
    11161 tgccatgcaa atgctcagta caggtcagtt ttcgaatata gaacaagcaa gaaatgtaat
    11221 caaacagtcg tttccatgtg aaacttttta cccagatgaa aataagcaaa acacgatgga
    11281 atggtacgtg aataaaatca aaggagtaat cgtatgaaca cgattgaaag aaattacgaa
    11341 caagcaaaag aaaaatatgc aactatcggt gtagatacag atgctgtact agaaaagata
    11401 caggacatca aaatttctat gcattgctgg caaggagatg atgtcaaagg tttcttgaca
    11461 ccggatggcg aattgaccgg tggaatcatg gcgacaggga attttccagg agccgcacga
    11521 acacctgaag agcttagaca agatctggag aaggcttatt cgttgatacc gggcaaacac
    11581 aaattgaacc ttcatgctat ctatttggat acagaagaat ctgtcgatct aaatgaaatt
    11641 gagccgaaac attttgaaaa atgggtagag tgggcaaaaa aagaagaaat cggtcttgac
    11701 ttcaatccga ctttcttttc gcatccaatg atgaaagacg gctttacatt ggctcatcca
    11761 gataaagaag ttcgtgaata ctggatcgaa cacgggaaaa gaagccgcaa aatcgcggag
    11821 tactttggga aagaaactgg gcaaacaagt atcaataact tttgggtacc tgatgggatg
    11881 aaagataatc cgattgatcg ttttacccca cgggaacgtt taatggaatc actcgatgaa
    11941 atttttacag aagagttaga tgaacggtat gtacaagatg cagtagaaag caaactattt
    12001 ggtttaggcg ctgaagctta tacagtcggt tcccatgaat tttatatggg ttacgggttg
    12061 actcgtgaca aacttgtttg tctagatgct ggacattttc atccaacaga agtgatttcc
    12121 aataaattat cttcattagc tttattcagc aaaggaatca tgttgcatgt ttcccgtcca
    12181 gtgcgttggg attcagacca tgtggttttg atggatgatg aattgcagga aatcgctaaa
    12241 gaattagtcc gtaacgaatt gttggaaaaa acaaatatcg gtctggattt ctttgatgca
    12301 acgatcaacc gtatcgctgc ttgggtcatc ggtacgagaa atacacaaaa agcactgctg
    12361 aaagctatgc tggaaccagt tgagcgattg gaagaaatgg agctggcttt tgactttact
    12421 tcaagaatgg cttatacgga agaattgaaa gatttcccgt atgccgatgt atggaattat
    12481 ttctgttaca aaaatcaagt tccagttggt ttggattggc tggaagaagt ccaggaatat
    12541 caaaaagacg tgttggaatt aagaaaatag gagtgtggct atattgaaaa aaatagatat
    12601 tttacaagcg ccttatgtga aagaaatgat cgaaacgacg tcgaatcttt atcgcttggg
    12661 atgggatgaa agaaatggcg ggaatatttc ttatttacta aaagaagaag agatcacacc
    12721 gttcttggat cctgaaaaag tgatccgccg catctcgatg atttttgatg ccagccgttt
    12781 agcaggatgc tattttattg taactggttc aggaagatat tttaaaaatg tagcgagcga
    12841 acctgccgag aatcttggaa tcgttcgagt tgctgaagat ggcaaaacac tggaactttt
    12901 gtgggggctg gaaaacgaat gccttcctac aagcgagttg ccaagtcatt tcatgagtca
    12961 tatcgcccgt ttagaagtag acccagataa tcggatcgtt atgcattgcc atgctagtca
    13021 tctattagcg atgagtttta cgcatgaatt agacgaacgt tcctttagtc gtacgctatg
    13081 gcaaatgtgt acggaatgct tagttgtttt tccagaagga atttccatca ttccgtggat
    13141 ggttccggga acaaatgaga ttggggaagc aacagctgaa aaaatgaaag aaactcgatt
    13201 ggttttatgg ccacaacatg gaatctacgg gtcaggaaaa gacatggatg aagtctttgg
    13261 tttgatcgag acagcagaaa aggcagcaga agtctatacg tacgtcaaag cgcaaggacc
    13321 aatcttgcaa acaatcacgg acgaaaacct gtggcattta gcagatgcgt ttggtgtcac
    13381 tccaaaagca ggttatttgg aggaagttca tacgaaagca ggtgtctgac atggtgaaaa
    13441 aagctttttg catgaaagtt tacccagaaa aacaagaaga atacaaaaaa agacatgatg
    13501 aactttggcc agaaatgcgt acagcacttc gtgaacacgg ggtcatcgac tattcgatct
    13561 atcttgatcc gagtacaagt ttgttatttg gttacttagt gatcgaagat gaaaagttat
    13621 gggaaaaaat ggcaaataca tctatcaatc aaaaatggtg gaagtacatg gaagatatca
    13681 tggagacaaa tccagatgcc tctccagtca gttgggaatt gatgccagtc tttgaactgt
    13741 agcaacaagc aactgggcaa ccggttgctt ggggttcttt cgtttttatt gtgaaaaata
    13801 atacaaatta ggaggaatag aaaatgagta atcgaatgat tttaaatgaa acatcgtatt
    13861 ttggaaaagg agcgattgag tcgatcgtcc ctgaatttca gcagcgagga ttcggcaaag
    13921 cagctgtcat tactgacaaa ggattgatcg agcacggtat agcaacaaaa gtaacagaac
    13981 ttctagataa agcagcaatc gcttatgctt tatacgacgg gatcgtacct aatccaacga
    14041 tccaaaatgt aaaagatgga gtggcttttg ttaaagaggc agaggcagac tgtttgatcg
    14101 caatcggtgg aggttcgcca atcgatacgg caaaagccat tgggattatt ttgacgaatc
    14161 cagagttttc agacgtcatt agtttagaag gtgttgctga tacgaaaaat ccatgtttac
    14221 ctatattagc tataccaacg acttctggaa cagcagcaga agtaacgatc aactacgtca
    14281 tcacagatga agtaaatcac cgaaaatttg tttgtgttga tcctcatgat atcccaatcg
    14341 tcgcgttcat cgacagcgat atgatgatgg gaatgccgaa aaaattggct gcatcaacag
    14401 gtatggatgc tatgacgcat gctattgaag ggtttatcac aaaaggcgct tgggaaatga
    14461 ctgatatgct gcatttaaaa gcaatcgaaa tcatcggcca ttctttggaa gcttctgtag
    14521 atggcgatca aaatgggcga gaaaaaatgg cattaggaca gtatattgcg ggaatgggct
    14581 tctcaaatgt cggtttagga ctggtacacg ggatggctca cccgttaagt gcatggtaca
    14641 acattcctca tggcgtagcg tgtgcagcac ttttgccaac tgtcatgaaa tataacaaag
    14701 aatatactgg agaaaagtac cgtgagatcg ctcttgttct agggattaaa ggagcagctg
    14761 aaatgtcttt agaagatgta agagaagcgg cttgtggtga gatcgatcgg ttgagcaaag
    14821 cggttggaat cccagaaacc atttctgaat taggtgtaaa agaagcagat attccagcaa
    14881 ttgcagaaga tgctttgcga gatgtatgca caccaggaaa tccaagagaa acgacagtag
    14941 aagaaatcat tgctttatat caaagtttga tgtaaaaacg aagcggatct tcaacattaa
    15001 attgcctgta aagagaaatg gtagtttata tctggatatc tcttttaaat tgatggtatt
    15061 tccgctataa tttttcttat aaagaaatag gagaatatta tatggaaaat aaaaagcctt
    15121 atggaactgt attgatcaaa gcctctaaaa tactagacta tttagcggaa aaccaagatg
    15181 tctctttgca gacgatcgca aagggtgtgg gaatgacctc ttctacagtc ttgaaaatac
    15241 tagatacttt gttgatgatc ggctacgtaa ataaaaattc agagaaaaac tatcgtttag
    15301 gtgcaaagct tattcgctac gcgaataaaa acattgaaca aatcgatttg gtagagttga
    15361 ctttaccgtt tttagaacag ctgcagcaaa agatcgatga gactatccac ttaggggtat
    15421 tggacaacaa tgagatcttg tacgtgaaca aactagatcc aaaacatcaa acgatccgca
    15481 tgtcttcaaa aatcggaatc acccgaccat tatacaattc tgcgatgggc aaagccgtat
    15541 tagcagaatt cagtgaagag cagtacactg attatcttga aaagcatccg ttgatccctt
    15601 acacagaata tacgatcacc aatgcattac ggttgaaaaa ggaaatcaaa acggtaaaag
    15661 aaacccacat cgcatttgac gatgaagaaa tcgaacgtga tattttttgt ataggagcct
    15721 caatcgtgaa aaacgaacaa atcattggtg catttagtgt aagtatgccg aaatatcgtt
    15781 taacagatga gacaaaagaa caaataatca gtgccctcaa gcaaacaaaa agcgaaatcg
    15841 agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt tttcttttct gccgtataat
    15901 atgggtatag atttccaagt tataaaataa aatttctaaa taggaaatag gtggagaaga
    15961 tgaaaaaaat tgatatttta tctcgattga aaaacgccgg agtgattgcg gtggtacgcg
    16021 gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat catcaaaggt ggtctaactg
    16081 gtattgagtt gacttttaca gtccctcaag cagatcaagt aatcaaagaa cttttgtcat
    16141 tctacaaaga ccaaccagaa atagtgattg gcgcaggcac agtattagac gctgtcacag
    16201 ctcgcttggc tatcttagct ggcgcagagt atattgttag cccttccttt gatcaagaaa
    16261 cggcagagat gtgcaatctg tatcaaattc cttacttacc aggatgtatg actattaccg
    16321 agatcaaaac agcgctgaaa agcggggtag atatcgtcaa actatttcct ggaagcgctt
    16381 atggaccaag tatcatttct gccttcaagg cgccgatgcc tcaagtcaat atcatgccaa
    16441 ctggcggtgt gagtctggac aatatgaaag agtggttcga tgctggtgtc gtaacagtcg
    16501 gagtaggtgg gaacctttta gcacccgcag ctacaggaga ttttgacaaa gtaacggaag
    16561 tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag gtagctgctt atgggaaaag
    16621 tagtgacact cggagaaatc atgttgcgat tgtccacaga gcagggaacc cgtatcgatt
    16681 caagtgaacg attcgctgcc cattatggcg gaggggaagc aaacgtagca atttctttag
    16741 ctaattacgg acatgaagcg atttttgcta caaaagtacc tgataacgca ttgggaaaag
    16801 cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa acaggtatta tctggtggat
    16861 caagactagg tacttattat atggaaactg gtagtggcga acgcgccgcc tcagtgatct
    16921 atgatcgtgc cggatcaagt tttgctgaaa tggatcattt ggaatggtcg ctagaggagt
    16981 tattccaagg aatagatata tttcatgttt caggaatcac cccagctttg tctaaaaaat
    17041 ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa agctggctgc ctgatcagct
    17101 tcgatatcaa ctatcgtgga aaattatgga cacagcaaga agccagcgaa gtaatccatc
    17161 agcttttacc tttagtagat atctgctcag ctggaaaaat ggatgctctt tatttgttag
    17221 gtatcgagaa agcgccagaa gcagaagaac aacctctaat ttattattat caaaaaatgc
    17281 aggaaaagtt cccgaatatt caggtgtttt attcgaccaa gcgtaccgtt cattcagcaa
    17341 gtgaaaatga attgaagggg acattgtgga tgaaccatga atatgtggaa tcccaagtcc
    17401 atagaatcac cccaatcgtg gatcgagtag gtggcggaga tgcttttgca ggtggcgtgc
    17461 tccatgggat tttatccaat atgtctccgc aagaaatcat tgattttgct acggcagctt
    17521 ctgccttgaa gcatacgatc catggggact gcaaccaatt tagtcaagaa gaagtcgcac
    17581 aatttttagc atgcggctct ggaaaaatca ttcgatagaa ggagtagaag aaaatgcaag
    17641 aaatggaaac acgttataca cacagtccag aagatatccg tcattattca acggaacaat
    17701 tgagaaaaga atttttagta gaaaaggtat ttgaaccagg gaaaatcagt ttgacttata
    17761 cgcacaacga tcgaatgatc tttggtggag tgacacctac tgaaaaagaa ttagaaatca
    17821 cattagacaa agaactaggt gttgattact tcttagagcg tcgtgaacta ggtgtgatca
    17881 atatcggtgg accaggattc atcgagatcg atgggcaaaa agaagaaatg aaaaaacaag
    17941 acggatacta tatcggaaaa gaaacacgac atgttgtatt ttcttctcaa gatgctgaaa
    18001 acccagctaa attctatatt agttctgttc cagctcatca taaatatcca aatgtaaaaa
    18061 tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg cctgacactg aatgaacgta
    18121 aaatctacca atacatccat ccaaatgtat gcgaaagctg tcaattacaa atgggttata
    18181 cgattttaga accaggaagc gcttggaata cgatgccttg tcatacacat gaacgtcgta
    18241 tggaagctta tgtctacttt gattatgcca atgaagatac acgtgtgttc cacatgatgg
    18301 gcaaaccaga tgaaacaaaa catttagttg tagacaatga acaagccatt atttcgccaa
    18361 gctggtcgat ccattcaggt gtaggtacaa gcaattactc attcatctgg gcaatgtgcg
    18421 gagaaaatat cacctataca gacatggata tggtgcaaat ggatcaatta aaataagcat
    18481 aagggagaga aaatgatgga atttagtatg gatatgtttc gtttagatgg aaaagtagca
    18541 ttagtcaccg gtgctgtcta tggtattgga tttgagattg cacgctcttt agctacagca
    18601 ggagcaaaaa tcgtatttaa taacttgaat caagaatcag ttgatgaagg tatagcgcat
    18661 tataaagaag caggcatcga tgcaaaaggt tatgtctgcg atgtgaccga tgaagaagcg
    18721 gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt cagttgatat tctagtcaac
    18781 aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg atgcggcaga cttccgccaa
    18841 gtaatcgacg tggatttaaa tgccccattc atcgtttcaa aagcagttat tccggatatg
    18901 atcgaaaaag gcggcggtaa aatcatcaat atctgctcca tgatgagtga attaggacgc
    18961 gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa aaatgctgac gaaaaacatc
    19021 gcttctgaat acggtcaata caatatccaa tgtaatggaa ttggaccagg ttatattgct
    19081 acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg gtgaacgtca tccattcgat
    19141 caatttatcg ttggacgtac gccagcagca cgctggggcg agccaatcga tttggctgga
    19201 ccatctgtct tcttggcttc tagcgcatct gatttcgtta acggccatgt cttgtatgtg
    19261 gacggaggca tacttgccta tatcggaaaa caaccttaaa agagctttag tacactgttt
    19321 agtggagagg ttgtgaaatc agcggattga acgaataccg aaggacagct gattaaacat
    19381 catttattcg aaaaaagagg ctgggacaga agcatttaac tccaagaaat aagaaggaat
    19441 tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttt
    19501 tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
    19561 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
    19621 tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt attcgttgcg
    19681 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
    19741 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cacggcattt
    19801 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
    19861 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
    19921 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
    19981 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
    20041 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
    20101 gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
    20161 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
    20221 gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
    20281 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt caaggcagcg
    20341 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
    20401 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
    20461 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
    20521 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
    20581 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
    20641 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaagaatgc
    20701 cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca gaagattttg
    20761 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
    20821 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
    20881 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
    20941 tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg tgaacttgat
    21001 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
    21061 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
    21121 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
    21181 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
    21241 tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg taaaaaacat
    21301 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
    21361 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
    21421 tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga aaaggatttt
    21481 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
    21541 attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact gtcacgaatt
    21601 gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat tctccggcaa
    21661 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
    21721 caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat ttggtaaatg
    21781 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
    21841 ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca tccatcgctt
    21901 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
    21961 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
    22021 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
    22081 atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
    22141 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
    22201 aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa atacttcggc
    22261 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct aatccaagct
    22321 taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag gaggctaatc
    22381 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
    22441 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgcaagg
    22501 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
    22561 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
    22621 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
    22681 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
    22741 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
    22801 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
    22861 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga ttcatccatt
    22921 gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg cttcgtcaag
    22981 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
    23041 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
    23101 gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata aatcggctgc
    23161 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
    23221 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
    23281 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
    23341 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
    23401 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
    23461 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
    23521 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
    23581 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
    23641 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
    23701 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
    23761 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
    23821 actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata ttgggggtat
    23881 atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
    23941 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
    24001 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
    24061 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
    24121 gttttgcaag cataaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
    24181 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
    24241 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
    24301 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttggagcgta
    24361 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta tacgattatt
    24421 acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa agtccaaaaa
    24481 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
    24541 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc atttgattta
    24601 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
    24661 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
    24721 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
    24781 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
    24841 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
    24901 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
    24961 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cgaatgccag
    25021 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
    25081 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
    25141 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
    25201 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct atttatcgca
    25261 agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca cgacaagcag
    25321 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
    25381 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
    25441 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
    25501 ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt tggatgctta
    25561 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
    25621 gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt gagataaagc
    25681 gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
    25741 cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
    25801 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
    25861 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
    25921 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
    25981 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaaac
    26041 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
    26101 gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct catcttctaa
    26161 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
    26221 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg ctagaaagtc
    26281 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
    26341 attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc gcttctcgcc
    26401 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
    26461 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
    26521 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
    26581 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
    26641 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
    26701 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
    26761 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
    26821 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
    26881 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
    26941 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
    27001 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
    27061 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
    27121 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
    27181 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
    27241 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
    27301 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
    27361 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
    27421 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
    27481 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
    27541 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
    27601 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
    27661 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
    27721 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
    27781 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
    27841 ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact gaacatttat
    27901 ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
    27961 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
    28021 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
    28081 atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt cggaaacacg
    28141 ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac gcatatctat
    28201 acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt tggtgccttt
    28261 ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa ggtagttgaa
    28321 cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac cgctgttata
    28381 ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt gttgccaatc
    28441 ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg ctcgattatg
    28501 gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta tgctggtttc
    28561 aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat gattgggatg
    28621 attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa aaagaaacgc
    28681 tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat ctcatggtta
    28741 gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat cttaggtttt
    28801 attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg ttataaagaa
    28861 atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc gccaatggtt
    28921 ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc tccttatttt
    28981 tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat cgtatttgct
    29041 atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc aggctcagct
    29101 attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt gttcccgtta
    29161 tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg ggttgcttgg
    29221 ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat cccaactggt
    29281 tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa cgcctagtaa
    29341 acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa cgttttgtgc
    29401 gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat aatgcgacgc
    29461 acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt ggagtagccg
    29521 caggtgtcgt tcagtctttt caaaattgtt tgctggaaaa taatatcagt ccagaagatg
    29581 ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa ggagacgtag
    29641 caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta gcaaaacgac
    29701 aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt gtcaatgcat
    29761 tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct tctttagaaa
    29821 gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat ggggaaccag
    29881 aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg gcttctgaca
    29941 tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat gcatctattt
    30001 taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa gcaggggtca
    30061 atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat gaaatgaaaa
    30121 aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc tctttgatgt
    30181 acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc aatatcgggg
    30241 tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac ccaacatata
    30301 tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta cgggctaatc
    30361 agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta gattatgcag
    30421 tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct cctaaagaag
    30481 gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg actattacaa
    30541 atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct tatggaaatg
    30601 tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg acggttgaag
    30661 acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg attttagaat
    30721 tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta gggggcggcg
    30781 ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg attcctgaaa
    30841 acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat gttgtagaac
    30901 gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa gcgatgaata
    30961 aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa atcgatccac
    31021 agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca accgatctaa
    31081 ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg cgattaaata
    31141 aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc gaacagaatc
    31201 gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc aaggtccaac
    31261 gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct gtcgaacaac
    31321 tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa ttttatctct
    31381 gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta cagcttttga
    31441 tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt gccggaaata
    31501 tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc cgaagaaatt
    31561 tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt gtccaagcag
    31621 aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag catcaaaaaa
    31681 aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc aatcgtttcc
    31741 tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga agatgaaggg
    31801 attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc ttcgatctct
    31861 aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat cgaagcttct
    31921 gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc tttttataca
    31981 catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc aaaagagatg
    32041 aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc atataatgaa
    32101 attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg tacggagtat
    32161 gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct ttttttccga
    32221 ataaacggtg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa caaacatgag
    32281 aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct cacgacctct
    32341 tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag ttaaaaacat
    32401 aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    32461 tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa aacggcaaaa
    32521 tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc tgattccaaa
    32581 acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta attatgaatc
    32641 acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa tccgacttca
    32701 tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa tactcgaccc
    32761 cagtctaatt ctgaaatcgt caaacgtaac catctgaaac ggttcaagtg cgtactataa
    32821 ctttctagcc aatcccaaat ttctcgtttt ccagcaattt cataacaagt tcggtgaaaa
    32881 tcagtatcca aatcaaaaaa agctttttta ttttgagcga ttactgcctg atcggtttgt
    32941 tctagtattt tttctagttt agcttgatct tgcttggtca ttttcgcact gcattcttgc
    33001 aggatcggtt tttccaactt ttcccgcgta taacgggcta atgaagcaga tcggatatcg
    33061 atttttgaaa cataagtgcc ggactgtgga atggttttta caagctcttg attgcgcagt
    33121 tggatcagtg cttctcgaat cggtgtacgc ctgatagcta gttgttcttc taacacttta
    33181 tcggagattt tacttccagg tgctaactcg gaataaatga tttgtttacg tatagattca
    33241 taagcttgct gttgcaaatt attgataacc atttaacctt cctttctatg cagatataat
    33301 ctattataac ataaatcgct tacaaatcgt gtgggcataa aaacacagtc tgcaaaaaaa
    33361 gccctgatag gcatgcctat tagagcctcg aaaagaaaaa tattgaatca atctggtcgt
    33421 acctcttgga tcggtttgac acgaccgccg attcttttag caaaagcttc ggattctttt
    33481 ttgtctttga aaatgagcag attgtctaag tttttttccc gagtgcgaca gccagtttta
    33541 tcgatgtaac cgtgcattac tttaacgaca tacatgggga tcacctgctt ttttcttttc
    33601 attatacgaa ctactcgaca caatacaaga aaaatgctta gacggtcttt attaagaatt
    33661 tacatcatcc aaagaagcgt acgcttggaa taactgatct aactccggat tgactttgct
    33721 cagcttcact aatcgttgcg aatcagaaag taaaaaacag tgtttactac tgccagtagt
    33781 ttttaatttt ttgtcttcag ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt
    33841 atacttttgg atagttggat ggatgctcac tttttctccg taatggatca tttctttata
    33901 gctgcaattt acttccagaa cggggatgat gatcccagat tcttcgattt ttttataggg
    33961 aaaccctaga tgtaccaata aggctacccg agcctcttca aaccagcgga tataattgct
    34021 gtgatgaata atccccattt gatctgtttc ataataaaaa ggttctcgca cataacctgt
    34081 aaatttctcc atgactcatg cctcgttttc cattgatttt ttacaaaaat aattcatcca
    34141 tttcggaagt gcttcgctga tttgtgtcgg caaaacaata tattgttctt ttgccagctg
    34201 gtctccaatg aaactatgaa gataaatagc tgctgcaatc gtttggtcat tttgaggaaa
    34261 ctgggctaaa aatcctgtga tcatcccagc taaagtgtca cccatgccgc ccgttgccat
    34321 agcagcagaa cctaaaggat tggtgtagaa gtgctggtga ccataaattt ttgtctcatg
    34381 acttttcaaa acgagcacag caccgatttc ctgttgcttc tttttgtttt ctttatcccg
    34441 ttgttcttgt atcggtagag aactcaaccg ttgccactcc atttgatgag gtgtaagtac
    34501 agtttgttcg ggatagggta gcaaaagatg attagcagca agtaaactta aagcagagcc
    34561 atcaatcacc agccattgtt cttttgattg gttttctagt acttgtttga aacgttccaa
    34621 actcttagaa gaagtaccca atccgggacc aatcaagagt acatctgctt gctttattac
    34681 ggaaagaaac tggtcagtat ccgtccaaac tacaatcatt gcttctggca gatgactgtg
    34741 aagagcactc cgattttttt catcagttac tacagtcgtg agacccgctc cgctgtgtac
    34801 ggcagctaaa gcactcataa tgattgctcc gccatattgt tcattgccgc caatcaaaac
    34861 gatacgtcca aatgttcctt tatgtgaaat ttttggacga ggtgtgatga ctcttttcac
    34921 tagttgttcg tttatcagca tctaaaaacc tccttttact cattatagcg tatatttttt
    34981 ttagagaaaa acagataaga aaaggaattg tcagattttt tgaactattt ttttctgtaa
    35041 tttgtggtat catgaacacg aagacttaaa taagtggagg attaacttta tgacaattga
    35101 ttggcaaaaa gaagtagaag cacgtaaaga agacttgctt gaagacttga aaaatctttt
    35161 acgtgtcaac agcgagcgcg atgactcaaa agtaacacca gatgcaccat ttggacctgg
    35221 acctcgcgac gcattgaaac atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa
    35281 aaatgtcgat aactatgcag ggcatattga tcttggcgaa ggagacgaaa cgctagggat
    35341 atttggacac atggatgtcg ttcctgcagg agatggctgg gacacagatc cttatgaacc
    35401 agtaatcaag gatggaaaga tctttgcacg cggatcaagc gatgacaaag gtccaagtat
    35461 ggcagcttac tatgcgatga agatcattaa agaacttgac ttgaaactat caaaaaaagt
    35521 tcgcttcgta gtcggcagcg atgaagaaag cggctggggc gatatggctt attatttcga
    35581 acatgaagaa gaaccagatt ttggtttctc accagatgct gaattcccaa tcatcaatgg
    35641 tgaaaaagga aatgtttctt tagctttacg cttcaaaggc gacaatgcag gagattatgt
    35701 cttgaaatct tttgtttccg gacttcgcga aaatatggtg ccaggaacag caactgcagc
    35761 acttcaagta ccaagtgcag atgcagctat cgcgatggaa gaagcatttt accaattcat
    35821 cgaagcaaat ccagtaagcg gaacgattga agcggacaat acgtatgtaa aaatcgaatt
    35881 agttggtaaa ggcgcacatg gtgcaagccc acaatctggt atcaatgcag gttctttctt
    35941 ggcattgttc ttagataact atgaattcct aggatctgct aaacaattta tccatgtagc
    36001 agctgcttat gtccatgaag atttttatgg agaaaaacta ggagtcgctt atgaagatga
    36061 aaaaatgggc aaattgacaa tgaatgctgg actatttgct tttgaagaaa atggtacaga
    36121 ggaagcgaac ttcatcaata tgaatttccg ttttccaaaa ggtgtaacag tagacggatt
    36181 gcaaagtgac atcgaacaaa cagtggggca agaaggcgca acagttactc gaggtgctcg
    36241 cgtaatggaa ccacattacg taccaatgga tgatccattg gtggctacat tattgcaagt
    36301 ctatgaagac cacactggag aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg
    36361 acgtttgttg aaacgtggcg ttgcttatgg agcgatgttc ccaggatata ctgatacaat
    36421 gcatcaagca aacgaattta tgagtcttga tgacttgttc agagcaacag caatctatgc
    36481 agatgccatc tatcgtttgg cgaaataatt aattaaaaag aggatcaaga attgcaaatt
    36541 atttttgcag tcttgatcct ttttttactt tctactgtgg tattcatgat aaacatgata
    36601 tagattcaat tcatattctt tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt
    36661 tactaatgaa agaagtgcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg
    36721 tgtaataaag cttgtcagca tatattcgcg aatcacagga atccccgtga ccaatccaaa
    36781 taaaaagaag aagatggtcc aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat
    36841 tttaataatc atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct
    36901 gccttcagga cgatcacgat aatcgattgg gatttcaacg aacttgaatt ttttatctaa
    36961 agcatggatc gtcaattccg tttcgatttg aaaaccagaa ctcataatag gaaaactttt
    37021 gacaaagata cgattgaatc ctcgatagcc ggtcatgaca tcaagaattt ttgttttata
    37081 cattctagta attgtatttc taacaaggtt atttccaaaa tcgtggaaag gtctcttatt
    37141 ctcatcaaag taggttccat ttgataggcg atcaccaatg accatatctg cttcaccaga
    37201 acgtaatttc tcgagcaaac catgtacagc ttctgcagga taagtatcat cgccatctac
    37261 cattaaataa tagtcggcat cgatatcaaa gaacatttgg cggatcacat ttccttttcc
    37321 ttgacgaggt tcttttttta cgattgctcc accttgaacg gccaattcgt atgttcgatc
    37381 ggtcgaatta ttatcataga cataaatatc tgcttctggt aattctcgtt taaagtctgc
    37441 aatgactgtt gaaattgttg cttcctcatt gtaacaagga atcagaacag caattttttc
    37501 ttgttgttcc atttttcact ctccttaact atagaaaaca aaagctaaat taaaaatacc
    37561 tcataatcaa aggaaatgct agtttttccc gctaaatatc atttatttaa aaagaaaata
    37621 ctggaatata tgtttggaaa gctctatcta aaaaaccacc cactacaaaa gaaggacatg
    37681 atacctctct tttgaatagt gggtgcgtat tcgttctctc acttttttga cgtattaatc
    37741 tgttgcacct ttttttcttg aaatttctaa tacggttcct gtatgagcat ctgctaaaaa
    37801 ttcataataa acagtttcgc catcttctat tcgagaaaga cctccccgat aagcactcga
    37861 ccgaacggcg aatttcctaa taggttcctt ttcaaaagag atccaagaac cttcgattgc
    37921 accttctttt agaaaagatg acttgatatt ttcaagtact tggtctgctg acaatacttt
    37981 tttgcgatga taccaaatcg ttgaagctac cccgctcaat aatcctagac cgattccaac
    38041 tgctaacccg cctttaaaat agcttagttc gctttcttca tacatgtgga catacctcct
    38101 tcttctcact tgttactcct tgtgcttatt ttaccacact ctcatatttt ctttgaaata
    38161 aataaccgct accagaaaaa ataagctata ataaatacga tcggtctgca gaaaaaatca
    38221 tattttatcc gcagatcaat ataagtctga gaggagttta gtctgaaatt tagacgaata
    38281 gcatatggaa gaaaaaacat ttcaacgaat caaagagtta acagaattac aaggaacaag
    38341 cggttttgaa caagatgttc gcgcttatat ggaaagtcat atggaaccgc tggtagatga
    38401 gttacagtta gatggtttgg gcggcatttt tggtctgcgc catcatgaag aagcggatgc
    38461 accccgtgta atggtcgcag cacatatgga tgaagtcgga ttcatgctga cacaaatcca
    38521 agaaaacggg ctttttaaag tcgtacctct aggcggctgg aatccttacg ttgtttctgc
    38581 tcagcgtttt acacttaaaa cctctactgg taagaattat ccatgtattt cttcttccgt
    38641 tcccccacat ttattgcgag gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt
    38701 gttcgatgct gggtttgaat caagagaaga ggcaatgagt tacggtgtcc ttccaggaga
    38761 cacaatcgtt ccttacgctg aaacgatcaa aacagccaac ggaaagaata ttatcagtaa
    38821 atcatgggac aaccgctatg gctgtacaat ggtattagaa gctttagaag cgttgcaaaa
    38881 tgaaacatta ggtcatacat tgattgcagg cgcgaatgtc caggaagaag taggcttgcg
    38941 tggttctaaa gcttctgtca ataaattcaa gccagatctg ttctttgcag tagactgctc
    39001 tgcagcagat gatacagtga cgaaaaatgg cacattcgga catttaggag aaggtacatt
    39061 gatgcgtatc caagatccag gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga
    39121 tatcgctgaa acaaacaata tcccttatca atactttgtt tcaaaaggcg gtacagatgc
    39181 aggtgctgct catacacaaa acgaaggaat cccaagtaca gtgattggcg tagtgggacg
    39241 ttatatccat acgcatcaaa cgatgttcag tattcgcgat tttgaagctg cacgagaaat
    39301 gttgattcaa acactgaaag gtttggataa atcaacagta aatacgatcg tatacggaaa
    39361 ataatgcgat agtgaaacta taaaatctgg caaaagattg ctttttgcca gatttttttt
    39421 ataaaaagag gatcaatgtt tgttagttcg aaagaaaggt gtataattta attgaaaaat
    39481 acataaacag aaagtagagg acaaagaatg attatcccaa atagtttaga agaattagca
    39541 ggctacgtag aaactggaaa gagtgtattt ttctttactg ccggctggtg cggagactgc
    39601 cgttttatca aaccgcaaat gcctgaaatt gaaaatgact tttctgattg gcaatttatt
    39661 gaagtagacc gtgacaagta catagatgta gccgctgaat ggaacatttt tggtatccca
    39721 agttttgtcg tgatccaaga cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa
    39781 acaaaacagg aaatcgaatc gttcctagaa agtgtaacaa aaggctgaca tctgttagga
    39841 ggggtcaaga tgaatcaaga atataagaat attttagtag gtatcgacgg tagtgaacaa
    39901 gcacttgctg ctttcaaaaa agcggtagaa gtggctcgcc gtaataatgg gaaagtatat
    39961 gtagcaaacg taatcgatca gcaattttat aactttatgg gctatgcacc tattgatcaa
    40021 aatctagtcg atcagcaaac tgaaacagcc aaggaaatga tcgaagaatg taaagaatat
    40081 gggaaatctg ttgattacac aaatatagaa ggaattattg catatggttc tgttcgtgaa
    40141 tccatggcag tcaaattgcc aaaaaaatat gagatcgatc taatcatggt tgggcagtca
    40201 ggtctaaatg cagtcgaacg ctttatgaca ggaagtgtag ccagctacgt gatacgtcat
    40261 tcaccttgtg atgtcttgat cgtttctgac gaagaagaac agccaagtaa ttaagcaagt
    40321 ggaacttgta cgaccttttt aatcaattaa ctaaaacagt aaagggactg tgacaaaagt
    40381 cttgtcacag tcccttgtta cgaataaatg gtgttccaga agaggcttct tcggaaataa
    40441 agcatgaaaa aaatttcatg cagtgaatgc aattttttgt taagataaat ctcagcaaag
    40501 gataatggag gaaagaacat gatttttgct tataacaaag cacacgtcgg agacaccttg
    40561 atggttatta tagccgacga taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt
    40621 gctcggatca aagatgaaca aggtcaagtt gttgcttgga actttttcca tatctccgat
    40681 catctaacaa tcgaagggaa cggccaagta agcttaacag atgaggaatt agcgaaattg
    40741 aatcgattga tcaaagaagc aggattcgaa gaacagcttg aagctgatca tgaaccgaag
    40801 atcgttgtcg ggtacgtaaa aacatgcgtc ccacatcctg attctgacca tttatcgatt
    40861 actgagacag aagtagacaa tggtacagta ttgcaaatcg tttgcggtgc acccaatatc
    40921 gaagctggac aaaaagttgt cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg
    40981 atctggccag gaactttgcg aggggtagat agctatggaa tgatctgttc cgctcgtgaa
    41041 ttgcatttgc caaatgctcc agcaaaaaaa ggaatcttag tattgcctga ggatgccgta
    41101 gtcggagaag catttccaaa ataaagaggt tgtgacaagt ctttgtcaca acctcttttt
    41161 tattaatttt cactattttg attttgtttt aatgccgatt gatcgatcgt caattcaaca
    41221 gtagttgttt tttctttcga gccttcatag taagtgattt tgacagaatc tcccactttt
    41281 ttcttataca aagcagattg aagttctaca ccagaagaaa cttctttgtc atcgatctta
    41341 gtaatgacgt cgtattgttt caatccagct ttttcagccg gtgtagctgt ttggacattt
    41401 gtcacgatga caccgttagt tacagatgaa ggaatcttca agatttgttc ttgttgctga
    41461 gtagaaacgg cagataagtc gaccattgtg atacctagag ctggacgtgt cacttttccg
    41521 tctttttcca gttgattgat gatattcact acatcgttac ttggaatagc aaatcccatt
    41581 ccttctacgc ttacattaga agtagattca gaagtacttg cgatcttact tgagttgata
    41641 ccgataactt gtccttcgat attaactaaa ggaccaccag aatttccagg gttgattgct
    41701 gcatctgttt ggatcgcatt gatattgact gtttcgttcg attcattcgt acttgtcact
    41761 tgtcggttca aggaagaaat gattcctgaa gtgacagaat ttgcatattc agatcctagt
    41821 ggggaaccaa tcgcaatcgc aggctcacca accttcagag cacttgagtc accaaaagag
    41881 gcaactgttt cgactttgtc tgaattgatc ttcaacaccg ctaaatctga ataagcatct
    41941 gttccaacta attcagcttt taccttcgta ccgtctttca tcaagacttc aagaccttgc
    42001 tggccatcta ctacgtgatt attcgtgact acataagcag tattcccgtc ttttttgtag
    42061 atcacgccgc ttccttcact ataagcttcc aaattactat catctgagct gctactttct
    42121 tcttgctgcc cgaatagtcc gccaaaaccg cttgattggt tttgactttg taagttgata
    42181 acagaaacga cagcgtcttg aactttatct accgctttgg tgatatcaga atctacattg
    42241 actttgacat tttccacaac tgtttcaccg gcagaattct gattgccgct tgtagcagta
    42301 gaactattgt tgccagtgcc catcgctgca taaaaaatgc cagctgtcag tagtcctcca
    42361 acgatccctc caacaagccc cagacctaac ttacgccaga ggctattatt tttacttttt
    42421 ttcatctttg gtgtcacatt ttttctatcc atgtgttcct cctccaatta cattcattta
    42481 tctactgtta gtatagtctt atttgttgac tttagtctac tttgagactg tgaacttttt
    42541 atgaaaaact ttaggaaaaa tcattgtttt cagatgaaaa tatttgattt tctttttcca
    42601 cagatactgt gtaaaactac gcggatagga ttggaaaaat aagggataaa acatatccac
    42661 agcctgtgta taaagtggat aaattcttat aaaacgtatg tttgcctaat gaatgtgatg
    42721 aaataaggta aaataacact gttttctgtg gataaacctg tggatgttgt gcataactgt
    42781 tggggaaatc tttttaacaa aaatagccaa aataaacata tagacgaaaa aattgattat
    42841 aataaacaaa gtgagcatga agaccaaagg agagaaaaca tgaatatcaa aattatctca
    42901 gtaggaaaat taaaagaaaa atacctgatc caaggtatca atgagtatgt caaacggctg
    42961 aatgcctatg caaaaatcga attgatcgaa gttcctgacg aaaaagcccc ggagaattta
    43021 agcgaagcgc aaatgagaca agtaaaagaa aaagaagggg aacggatctt agcgaaaatc
    43081 aaagaacaag aatacgtcta tgccttagca attgaaggga aaaatcctac aagtgaatct
    43141 tttgctaaac aaatcgacca attaggtatc caaggaaaaa gccaccttgt ttttgttatc
    43201 ggcggctctt taggactcag cgaagccgtc atgaaaagaa gcaatgctca gatctctttt
    43261 ggaaaaatga cgtatccaca tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt
    43321 gccttccgga tcaatgcggg ggcgccttat cataagtgat gcggttttta tcattgatcc
    43381 taaaaatctt agcaagacaa aaagtacgta agaaagtaaa gactaaatac ttcggaggaa
    43441 caaatgaata acaatgatag gttattgcga ttgcgttatg cgattgattt gaaagatacg
    43501 gatttgatta aagcttttga gctaggcggt gtaccactga ctaaagaaga agctcaggcg
    43561 gtactgacca aagtacagga taagaacaaa gataatgcag aaaataatgt gtatgaaaaa
    43621 accatcaaca accaagtttt tgacgcattc ctaaatggcc tgattctctt ggcgcgtggt
    43681 ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa gcaaggaaat caaatatatc
    43741 aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta tgaccactga tgatatttta
    43801 gacgtttttg ctgaggcaga aatctatcca tcaaaaggcg aaatcggggc atttttgaga
    43861 aaagaaggcc aacgaaattt taagccttgc ggagataaat atatgcgtaa ttttctcaag
    43921 gggttaggaa tttataatcg gagaaaagta taaagaaatt tcatgagaaa tcaaaacaat
    43981 ggttgttttg atgagagcta gtttttgtat gagattaaaa tattatctta atcgaggagc
    44041 tggattggtt ttatgaatga tgaatataag aatgatgaag ataagatgct ttttgaagag
    44101 atagagaatc gatgtaggct caattttgag cttcggggaa agatgtcttt aatacaacaa
    44161 aaaaagtatt tagcgaataa atcagagttt actctgggac atgttgaaaa attaatctcg
    44221 gattggatta gtagtagaag cgaatttaca aaaataaaac agccgattaa atttgacatg
    44281 aaaaaacttt tgttaaataa gagtgagata ggtaatagag atcaatatat tagagctaaa
    44341 gggcaagaaa taattgattc attaggtgaa atgaggtcat acaattattt gtatgtaaca
    44401 catagagctg atggaatggt cattactgtg gggaaatctt cttctaatga tattttttta
    44461 gatggagatc ttttttatca actcaatata aatcacttgt ctggcactga aaat
//
LOCUS       DADJYG010000010        42828 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.010, whole genome shotgun sequence.
ACCESSION   DADJYG010000010 DADJYG010000000
VERSION     DADJYG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.010"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(73..1176)
                     /locus_tag="JZ382_000452"
     CDS             complement(73..1176)
                     /locus_tag="JZ382_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAY2426663.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1805..2275)
                     /locus_tag="JZ382_000453"
     CDS             complement(1805..2275)
                     /locus_tag="JZ382_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAY2426664.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            2605..4245
                     /locus_tag="JZ382_000454"
     CDS             2605..4245
                     /locus_tag="JZ382_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY2426665.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            4399..5721
                     /locus_tag="JZ382_000455"
     CDS             4399..5721
                     /locus_tag="JZ382_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAY2426666.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5789..6223)
                     /locus_tag="JZ382_000456"
     CDS             complement(5789..6223)
                     /locus_tag="JZ382_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426667.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(6288..7125)
                     /locus_tag="JZ382_000457"
                     /pseudo
     CDS             complement(6288..7125)
                     /locus_tag="JZ382_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(7867..8493)
                     /locus_tag="JZ382_000458"
     CDS             complement(7867..8493)
                     /locus_tag="JZ382_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426668.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(8788..9123)
                     /locus_tag="JZ382_000459"
     CDS             complement(8788..9123)
                     /locus_tag="JZ382_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY2426669.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(9110..9394)
                     /locus_tag="JZ382_000460"
     CDS             complement(9110..9394)
                     /locus_tag="JZ382_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY2426670.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(9450..10973)
                     /locus_tag="JZ382_000461"
     CDS             complement(9450..10973)
                     /locus_tag="JZ382_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY2426671.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10966..12141)
                     /locus_tag="JZ382_000462"
     CDS             complement(10966..12141)
                     /locus_tag="JZ382_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY2426672.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(12145..12330)
                     /locus_tag="JZ382_000463"
     CDS             complement(12145..12330)
                     /locus_tag="JZ382_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426673.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(12296..13990)
                     /locus_tag="JZ382_000464"
     CDS             complement(12296..13990)
                     /locus_tag="JZ382_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY2426674.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13987..14460)
                     /locus_tag="JZ382_000465"
     CDS             complement(13987..14460)
                     /locus_tag="JZ382_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY2426675.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(14529..14684)
                     /locus_tag="JZ382_000466"
     CDS             complement(14529..14684)
                     /locus_tag="JZ382_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAY2426676.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(14818..15198)
                     /locus_tag="JZ382_000467"
     CDS             complement(14818..15198)
                     /locus_tag="JZ382_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY2426677.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(15202..15417)
                     /locus_tag="JZ382_000468"
     CDS             complement(15202..15417)
                     /locus_tag="JZ382_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426678.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(15420..15827)
                     /locus_tag="JZ382_000469"
     CDS             complement(15420..15827)
                     /locus_tag="JZ382_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAY2426679.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(16093..17568)
                     /locus_tag="JZ382_000470"
     CDS             complement(16093..17568)
                     /locus_tag="JZ382_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY2426680.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(17558..18406)
                     /locus_tag="JZ382_000471"
     CDS             complement(17558..18406)
                     /locus_tag="JZ382_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAY2426681.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(18443..18805)
                     /locus_tag="JZ382_000472"
     CDS             complement(18443..18805)
                     /locus_tag="JZ382_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426682.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(19105..19729)
                     /locus_tag="JZ382_000473"
                     /pseudo
     CDS             complement(19105..19729)
                     /locus_tag="JZ382_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(19726..20037)
                     /locus_tag="JZ382_000474"
     CDS             complement(19726..20037)
                     /locus_tag="JZ382_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426683.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(20081..20374)
                     /locus_tag="JZ382_000475"
     CDS             complement(20081..20374)
                     /locus_tag="JZ382_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426684.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            20568..21215
                     /locus_tag="JZ382_000476"
     CDS             20568..21215
                     /locus_tag="JZ382_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2426685.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            21276..22421
                     /locus_tag="JZ382_000477"
     CDS             21276..22421
                     /locus_tag="JZ382_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2426686.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(22502..22807)
                     /locus_tag="JZ382_000478"
     CDS             complement(22502..22807)
                     /locus_tag="JZ382_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAY2426687.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(22832..23263)
                     /gene="ruvX"
                     /locus_tag="JZ382_000479"
     CDS             complement(22832..23263)
                     /gene="ruvX"
                     /locus_tag="JZ382_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY2426688.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(23260..23526)
                     /locus_tag="JZ382_000480"
     CDS             complement(23260..23526)
                     /locus_tag="JZ382_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAY2426689.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(23781..24506)
                     /locus_tag="JZ382_000481"
     CDS             complement(23781..24506)
                     /locus_tag="JZ382_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAY2426690.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(24867..24937)
                     /locus_tag="JZ382_000482"
     tRNA            complement(24867..24937)
                     /locus_tag="JZ382_000482"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24903..24905),aa:Gly,seq:ccc)
     gene            25054..25806
                     /locus_tag="JZ382_000483"
     CDS             25054..25806
                     /locus_tag="JZ382_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAY2426691.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            25822..26301
                     /locus_tag="JZ382_000484"
     CDS             25822..26301
                     /locus_tag="JZ382_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY2426692.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(26552..28132)
                     /locus_tag="JZ382_000485"
     CDS             complement(26552..28132)
                     /locus_tag="JZ382_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAY2426693.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(28125..28868)
                     /locus_tag="JZ382_000486"
     CDS             complement(28125..28868)
                     /locus_tag="JZ382_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426694.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(29044..29820)
                     /locus_tag="JZ382_000487"
     CDS             complement(29044..29820)
                     /locus_tag="JZ382_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426695.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(30068..30904)
                     /locus_tag="JZ382_000488"
     CDS             complement(30068..30904)
                     /locus_tag="JZ382_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAY2426696.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(30915..31844)
                     /locus_tag="JZ382_000489"
     CDS             complement(30915..31844)
                     /locus_tag="JZ382_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY2426697.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            32036..32389
                     /locus_tag="JZ382_000490"
     CDS             32036..32389
                     /locus_tag="JZ382_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAY2426698.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(32463..33119)
                     /locus_tag="JZ382_000491"
     CDS             complement(32463..33119)
                     /locus_tag="JZ382_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAY2426699.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            33345..35279
                     /locus_tag="JZ382_000492"
     CDS             33345..35279
                     /locus_tag="JZ382_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAY2426700.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(35365..36588)
                     /locus_tag="JZ382_000493"
     CDS             complement(35365..36588)
                     /locus_tag="JZ382_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAY2426701.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(36582..38480)
                     /locus_tag="JZ382_000494"
     CDS             complement(36582..38480)
                     /locus_tag="JZ382_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAY2426702.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(38474..39592)
                     /locus_tag="JZ382_000495"
     CDS             complement(38474..39592)
                     /locus_tag="JZ382_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAY2426703.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(39675..40508)
                     /locus_tag="JZ382_000496"
     CDS             complement(39675..40508)
                     /locus_tag="JZ382_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2426704.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(40501..41418)
                     /locus_tag="JZ382_000497"
     CDS             complement(40501..41418)
                     /locus_tag="JZ382_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2426705.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(41441..41620)
                     /locus_tag="JZ382_000498"
     CDS             complement(41441..41620)
                     /locus_tag="JZ382_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426706.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(41645..42769)
                     /locus_tag="JZ382_000499"
     CDS             complement(41645..42769)
                     /locus_tag="JZ382_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2426707.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        12828 a         8664 c         7127 g        14209 t
ORIGIN      
        1 gacatccttg aactataaaa ataggagtac ggcatcatct gccgcactcc tatttttttc
       61 gtcatttatt tcttaaataa aacgattctc acgcaatttt atttttttga tatcttctac
      121 tgtttgtgtt ccagctaatt gcatgaccat ttccaattct gttttaaaga aatcaaacac
      181 ttgatggact cctgtacttc ctcctaaaga caatccataa ataacgggtc tgccaatagc
      241 aacaagatcg gcacctgatg cgattgcttt gaaaacatgt tgtccacgtc gtacaccgct
      301 gtcaaacacg attggtacac gtttatctac tgcttctgcc acatattgca acgaatcaaa
      361 cgcagcaggc cccccatcta attgacggcc accatggtta gatacccaaa ttccaccagc
      421 accagattct aatgatcgat agacatcttc ttctgattga acacctttca cgtagactgg
      481 caaatcagaa tgagcagcga taaattctac atcttttgga ctaagttttt gtttggaaga
      541 tttatataca gcatccatcg tttggcctac tcctgattga taagcctgaa cgatcggcat
      601 tggcagcggg aaggtaaacc cgtttctgcg gtctgtttca cggtttccgc caactgttgc
      661 atccgctgtc aatacgattg cttttgctcc attacgcttg gccacttcaa gaatatccaa
      721 attgattcca ttatctttgc tcatataaaa ttgaaaccat tgcggtgctt tctccccgcc
      781 tgcttctctt atctcttcta aagtacaaga agcgtaagaa cttgctgtat agatcgtccc
      841 aaaatctgct acaccttttg cagaagcttt ctccgccttc acatgagcta atccatgagc
      901 agcaactggt gccatgatga ttggtgcagt cagcatttct tcgtcaaaat gtgtagttgt
      961 atcgggtaat tctacatctc ttaatacatg aggaataatc aaacgatgat tgaatgcccg
     1021 ttcattttct tgataagtaa ataagtcgcc agcaccactg ctgatgtaac cataaccacc
     1081 agctggaatc acttgtgaag ccgcgatttc taaatcttcc atattgataa aatctactat
     1141 tccttcattt gtacctgctt gatatgtttt ttccatctca atgccttctc tctataaaat
     1201 ttgaatgatg aacaatatga tagcgtttac actttttata ttactcttcg acattgatac
     1261 aggcaaataa tctgtttaca tttcagccat tccagatgtc actactctgg aagaaatgat
     1321 tgtaataaaa atagataaaa aaccgatggg actttgacaa aagtagtgtc aaagtccctt
     1381 tttttgaata aacggtgttc aaaagccatt ttctcggtat taagtcaaat ttatgcaaaa
     1441 atgtggaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     1501 aacctctcga taaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaatggca
     1561 aaatatgagg aactattttg ccatttgtct ctctaatact caggtcagaa cgttgacttc
     1621 acagtctctt ccttaaacgg tgttcaaaaa cttgcatctg cggtaataag cccaaccaaa
     1681 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc agggcaggtt
     1741 tttcacgacc tctgattcac ggtgtttctt tttcatcaac atttttacga aatactgtgg
     1801 aagactattg aaataagtgt ggattgtttc tttgtgtttc aatcgtttct tgccattctt
     1861 catttaattt tggattaccg ttttcttcct tgaactcata aggtcctaat tcaaaaatcc
     1921 cagacagtat ttgattcacg atttcatata gttcagtgtg atcagacaca tgattcttga
     1981 agatcatctc attatcacca cgtttgaacc tcacattcgg gctctcatga tgaacctcta
     2041 tgcatttttc tccttttttt aactgacgga cttctgaaaa catctcataa atatcttctc
     2101 cagtaaaacc ttcagcaagc gccagactgt ccacaagacc gcctactgtc agttcagaga
     2161 ttattgcttg attggaatct aattttaatg tatagcttgt cgcttctact acgccacatg
     2221 cattgcaacc gccttctgat agttcgcttt ttctaatcaa ttgaacttct ttcattttct
     2281 cttcctcctc ttaattttct acttatgtat ttttatcacc tgacataggt gactcctatc
     2341 aacgctatca tgtgcttttt tcctcagaaa tcctatttat ccattccatc tatcccttat
     2401 tcttctactt ttctttctct agcatataag agatacgcat agtattcaag caaggattgt
     2461 cgatataaaa agaacaaata ctgctatttt gatttctttt ggctatatta catataaata
     2521 aaatttatca tacctgctct ttttgactat atcatcatct gacttctata cttcgtttgt
     2581 attaaaactt aggaggaatc attcatgctt acagggttaa acttattaaa taatcctttt
     2641 ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa aatatggaat cactggtatg
     2701 cttccaagta ctgtccaaac actggaacaa caatctgttc aagcttatgg tcaatactta
     2761 agcaaacaaa cagatttgga aaaacgtatc ttcttaatga atctattcaa tacgaatcgg
     2821 acattgtttt ataaattgat gggcgaacat ttagttgaat tcatgcctgt cgtttatgat
     2881 cctgtcgttg cggattcaat cgaacaatac aacgaaattt tcttgaaacc gcaagatgct
     2941 gcatttcttt caatcgacga accagaaaac atcaaagcaa gtttgaagaa cgcggcagat
     3001 ggacgagaca ttcgtttgat tgttgtgact gatgcagaag gaatcttagg tatgggcgac
     3061 tggggtgtga acggtgttga catcgctatc ggcaaattga tggtctatac tgctgctgct
     3121 ggtattaatc cagcacaagt tttgcctgta tcgatcgacg ctggtactaa taatgaagaa
     3181 ttattgaata atcccttata cttaggcaat cgtcatgctc gtgtagaagg cgaaacttac
     3241 tatgaattca tcgatcaatt cgttcaatca gctaccgaat tattcccaga attgttattg
     3301 cattgggaag attttggacg aggaaatgct gcagcgatct tagaaaaata cgaagatcaa
     3361 atcactactt tcaacgacga tatccaaggt acaggaattg ttgtcctagc aggtgtatta
     3421 ggcggattga atatatccgg tgaacaattg aaagatcaaa taattttgac atttggtgct
     3481 ggtactgctg gtgtgggtat cgcaaacatc cttttagatg agatgatccg tcaaggtgta
     3541 cctgaagaag aagcacgcca acatttctat caagtagata aacaaggttt attgtttgaa
     3601 gatacagaag gattgactcc tggtcaaatc ccattcgctc gcaaacgctc agatttcact
     3661 aatgctgatg aattgacaaa cttagaagca gttgtaaaag cgatccatcc gacaatcatg
     3721 attggtactt ctacccaacc tggagcattc actgaagaaa tcgtgaaaga aatggctgct
     3781 catacaccac gtccgatcat catgcctttg tctaatccaa caaaattggc agaagcaaaa
     3841 gcaaaagatt taatagaatg gacggacgga aaagcattag ttggaacagg tatccctgct
     3901 gctgatgttg aatataatgg tgtaacttac caaatcggtc aagcaaataa tgctttgatg
     3961 tacccaggtt taggacttgg tttgattgct tcaacagcta cacgagtaaa tgctgaaatc
     4021 atttctcaag ctagtcgagc attaggcgga atcgttgatg taacaaaacc aggagcagct
     4081 atcttgcctc cagtagcaaa aatcactgaa ttttctcaaa cgattgctga aactgtcgca
     4141 aaatcagttg tcgcacaaaa cttgaatcgt gaagaaatca cggatatcaa agaagcagtt
     4201 gaatctgcta aatgggttcc tgaatataaa tctttagaag attaagatcg ttatagtaaa
     4261 agaacataca gaaactttgt atgacgtcta aaaagaaaaa agcgactgcg gcaaaacagc
     4321 ggccagccaa ctcctgctct tttgtcgctt ttctttggct tttttcatat gatacagaat
     4381 aagaaaggat ctgtaaaaat ggcacaaaaa acaacaactg caactgcagt tgaggaaaag
     4441 aaaaaagaag gtttttggtc cacaaaaatc accggcgtaa gtttgccact ttatttgatc
     4501 atggtccttg tcctgatcat tgcaatggct ttagggaaat taccgacgaa tatgattgga
     4561 ccaatcgcag ttttggttat tttcggtaat ttatttcatt ttatcgggac aaaaatccct
     4621 atcgtcaaaa gttatttggg tggtggttcc gtttttgcga tcttcgcttc tgctgcgatt
     4681 gctacatttg ggatcttacc tgaatatgta gtgaaatcca cagaaaactt tgtcagcaat
     4741 atgggctttt gggactttta tattgctgcc ttgattactg gtagcatcct tggtatgaac
     4801 cgtaatctat tgatgaaagc ttctgttcgt tttattcccg ttgcacttat ttcaatggtc
     4861 gtctcctttt ttgccgtagg actcgtgggc atgcttatcg gaaatggttt tgcaaattcc
     4921 gttttataca tctcattacc tgcgatggct ggtggtgttg gagccggagc cgttccttta
     4981 tcaacgattt atgcaaatgt tttaggtacg gatgcttctt ctatcatttc ccgcctgatt
     5041 cctgcgacag caatgacaaa tgtcttagct atcatcggtg cagcattagt tgctcgtgct
     5101 ggacaatcaa tgccaaaatt caacggcaat ggtaaattaa tggaaaaagg cgatcttggc
     5161 gatggaaaac ctcgaactgt gaaaccttcc attccacagt taggtgttgg tttgatggta
     5221 tcattgactt tcttcatctt aggacaaatt ggtaactatt ttgttcctaa agtccatgct
     5281 tacgcattta tgattattat cgttgtcatc tgtaaaatcg ccaacctatt accagagtat
     5341 tatgaagatg cagctatcat gttcaataac ttgattgtca aaaacttgac agcagctgtt
     5401 cttgccggta tcggtattgc gttgctgaat ctgaatgtat tagcttctgc tttgacatgg
     5461 caatttgttg tcttatgtct gacaagtgtt atcgcgattt cagttgtttc aggatttgtc
     5521 ggtcgtttgt ttggtttata tccaattgaa tcaactatta ctgctggctt atgcaacaac
     5581 tcgatgggcg gaacaggaaa tgtagccgtc ttatctgcag ctaatcggat ggaacttatt
     5641 gcttttgctc aaatgggcaa tcgacttggt ggtgccatcg ttttgatcat ctctggtttc
     5701 ttgatgcaat tactttctta gtcaaaaaaa caaaaatacc tgatcatcaa gtatcggctt
     5761 ggtgatcggg tatttttttg ataaagattt acaggatttt caaaaatctt ttttgcattt
     5821 tatcggaatg ataaccgatg tgttcataaa attggtgtgc ttctactctg tattctgcag
     5881 aattaagcct tattgtcgtt attcctcgtt ttttcgcttc ttcttctacg ccagtcatca
     5941 aagcctttcc gatgcctctt ttttcagcat atttagacac agcaagtgcc ataatattga
     6001 acattggatc gctaaaaatc gattcatagg actccgcttg tacatagcca gctgcttttt
     6061 tagaagtagg atcttcataa accaagaaaa aatttttctc tggctccttc agcagtttct
     6121 tcatttgtct caccgtaacg gttaatggta tatcataccc taattgatcc gcattgattt
     6181 cacgtagtgc ttctgcatct tctaaacgca tttcccgtaa catccaaatc accagccaat
     6241 ttctgatttt ttaaaatgaa tttttttatc atagtatact ctttttttca tttagaacaa
     6301 tatgtttcag tgattttgac ttgcgttagt ctagattctc ctaatgtttg taagatgaca
     6361 aaggcattgt aacaattgac caaaagaacc acgaggacaa ttacttgaaa aataaaggtc
     6421 actttttttg gagacaaaac cctgctaaat cttgctcccc acaaacctcc aataacagca
     6481 gcggggataa cataaaacaa catagataaa tcaaagcgca taaagctact tgttaaagca
     6541 atcgttacca attttgccag ttgagaaaag aaaatcgtag atagagaata tagtgttgct
     6601 tcttttattg gcaatgcaaa catcagcatc aacaaagaaa cattgactgg tccaccacca
     6661 atacctaaaa aactagctaa aaagcccaga acccccccca catataaggt accaagtcca
     6721 tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt
     6781 caataccgtc aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa
     6841 aaaaacttcg aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca
     6901 accgacaatc tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat
     6961 ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc
     7021 gataagatca agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac
     7081 tgtgttcgcc agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc
     7141 tcttgtttat atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca
     7201 aacgaggctt cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaagaggt
     7261 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata
     7321 attttcgttt ggttgaactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     7381 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aacagcttct
     7441 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttaagg
     7501 aagagactgt gaagtcaacg ttttgacctg attattagag aaacaaatgg caaaatagtt
     7561 cttcatattt cgccgttttt cgagcgaatg ccgaggggca gctgattgaa catcgtttat
     7621 ttggaaaaag ggattgtgac aagacgtttg tcacaatccc tttttttata tcttattaat
     7681 tcattctttt gttctattct tcttcttccg ccatgaatga gaaacccata tcaaagaact
     7741 ataaaggtta tcaaaacagc gtttatgact aaatgaatta gagttatcta tcactatttt
     7801 tcaagcgatt taaaccaaat ggcatacaat atggcataca aaaacttcaa taagccgtgg
     7861 gggtttttaa atttcttctt gttctttaaa atcaatgagt ggagctacat aatttgaatc
     7921 cactttttca aacgtcactt tttgcatcga cgaatgtttt ctttcccaag ataacgtaaa
     7981 tagatttgac tctggactta taaagtctac ttcaccttct agtattactt tcttatgcaa
     8041 atattcctct aataaatata gacttgctaa agcatacaac acattttcca aatttgcttc
     8101 cttataattt gaagaacgac catgttttac tctgttatat attttccacc aatctggtgt
     8161 atcttcaggc cacccatctc caaaaggttt tagtgaagtc aatgttaaag aattcataac
     8221 cctaacacca ttatccaaac acattaattc gctagattta ataacagctt taaaatcagt
     8281 tatgctttta ttgctactgc tatcaaatcc gcaaagttct ttgaaaataa tctcacactc
     8341 cgaaccaatc gctagtagta gtttttgata ttcaatagag aatgttttga aattattctc
     8401 atcgagcatc acaaaatctg tggtattaat aaactcattt tctaaatgta gataatactt
     8461 ccaataaaaa tttgtaaact ctgctttgtc cattaaatca cctcttttga attataacaa
     8521 aaaaaacacg tccacaacag acgtgttctt tgatttctac acaatcagct tgccaaaacc
     8581 atccaatcag tcctagcatg cctgtattaa aattgaacgt tcatcatcta tcatctttct
     8641 tagtccagca tgtttgcaac cacacctaca attttcagtc caacaataga tttcgactag
     8701 ctaagtcttg caaaattcag gcagctaacc cttcatttac taccttaatt atactaaata
     8761 cccacaacgt ttacaagtgt aatcattcta agaaatctgt ttagctatga tcgttgtata
     8821 atctttaaac gccgttcctt cattgattga aataatttta taactcttgt ttttccaagc
     8881 tactttcata tcgtttgtta gttcataatc ttgatcgtat cgaataataa acgtcaacgt
     8941 tccttcaaga atggtaccta cactagcaag cacgtctttt agcatttgtg aacgcacatt
     9001 agcccaacat tcaaatacag tttcttcact atccaccatc tgcccatctt catcttttac
     9061 ccgttttgtc tttttaaact tgactctctc agtcaattca tttacattct taatgattgg
     9121 cataataagc acctctcatt tgttgtacta atgcttgtac cgttactggt atccgttcgc
     9181 tgctggcttc ttgacggttc aaataccaat gttgagtaag taatgacacc gccaaatcaa
     9241 attgctgata attatccata tctttatccg ttgcgctact atcaatagca ctttttatat
     9301 attcgcctgc tgtatcaatc aattgttgaa taaagtcatc atcaatagtg tgatcgatcc
     9361 gcatactttt ttttatgcta tccaaaatta ccattttatc tctcctaatg aaaaaaaggc
     9421 agtgggataa accgccgccc aatatttttc tatacttttg gtgttacaac tggcgtaaac
     9481 tcaatataca ctgaagcatt ttgatcaatt ttcttataat cgttgcgaac gatcactgca
     9541 agtccttgtg agtagtaatc gaatttttcc cattgagtcg ttacttgatt tctacgagca
     9601 acaaagatag attgtgcaat atcccccata atcataggga atgttccagc tttaggattg
     9661 gcaaacaatg tatctgcaat caatactact ggcataccga ataatgattt accactaggt
     9721 gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg
     9781 taatatccgc tttggtttag aattaccatt ttatttaatg ctgggtctaa tgccacattg
     9841 tatagttgtt tcaaatcgtc caacgtagca gccgttttct tagtgaatgt ttttaataaa
     9901 tccatgatat gcttattgtc agtgttttct accaatttcg ctaactgatc tttgacctct
     9961 tgtacaatat tcactgctga atcttccaca acctcatttg ataaggcaat cttaccagca
    10021 cgagtttcta ctttataatc aactgaagta aacatttcag cgtcaatatc accaatttca
    10081 gcaagttcag cttttgttgc taacactgct tgttggttag ttgctactgg atatttacct
    10141 tgtccatttg atactgtttt tactgttgca tattgagcta agttataatt tgaacgtttc
    10201 aaatcaaaga cttcaccgat tacttcttcg ggaacaactg ccgctgcatt tacagtagtg
    10261 actccatcac gcacttcgcc ttgtgaacgg atatattctt catacccacg caattctgtt
    10321 ttttcgttat cgattaaagt tttttccatg ttattactct ccttttgtgc tttagaagtg
    10381 gattctaagc tattgtttgt ttgttttgct tggttgttac tcataaacga ttcgtaagaa
    10441 cgcttattta cttggacatt tgatgaatca taagcgggaa cggtcacgac actaatttca
    10501 ttaagtgctt tgattttatc tattgatcgt tcaattgtgc catcttcttt tttgtcgaat
    10561 gaatctaatc caagcacgaa cccaaaggac atagaatcaa caaccccttt tgagatattc
    10621 tcatacacat catttgaata ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta
    10681 tcatcgacat tcaatttcaa cgtacctgct ttgacactag ctagtggctt tgaataatcg
    10741 tgtccatata ataagaacac atttgataaa tccacctctt ttaatgcttc gggtgtaatc
    10801 acttctacaa agccgcctaa atcttttgac ggttgcccga atttcaacgc atagcctgaa
    10861 atagttttgc cgtctgttgg ttgtttttca ttttctttct ctactttggg agaatcggct
    10921 gttagctcag cttcttctgt cagccgtttt tccttttctt cttcattatt caactggtgt
    10981 aactccttcc tgttctttta attgatattt ctccaacgta tctaaatacg taaagttcaa
    11041 acttgctagt aaacgatcgc caccatccat ggggggtagc cccattttcg accgcccctc
    11101 attaatcgtg agaagtgacc ctttgacctg atccaataca ttttttactt ttgtttcagg
    11161 atcagtctct agtaactgat cggaattaaa gcggatattg gtttcaagtt tcgtatctaa
    11221 ttcactcaca aacacattaa agtaatgaat cagcgtactt tggatatact ggagattact
    11281 ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc tcaatcggta caccaaacgc
    11341 cttggcaatt tgttttgtgt tccaatcatt ggaattgata agcttcaaaa catcggtgtt
    11401 tatttctaat gttttgtagt ccattgtttc atcaagaatg atcgttctaa gagcattatc
    11461 tccactactt gaaccgttgg cttcttcaaa cttttcacga attgcgttct ttgctgatcc
    11521 gtctaaatct gacttatgaa ccttcaaaat acctgaccca ctgacaccac gagtaaagaa
    11581 attgtgcaat gtgcggttgc cagcttgttg aattttcagt tcatcacgta acgcataaag
    11641 tggtggtaat cctgttaaac catcttgggt gaaatatttg aagtgcaaaa tatcgctaga
    11701 cttcacacgt ctttttttat caccaatctg ataagacaac gtaccattat ctaattgagt
    11761 aaccgttaca cttgagttag gtaataagtg aatttcttct accttttcac cgttgcgttt
    11821 aatctctgca aagctattac cgtttagcaa catattgacc gccaaagcaa actttaaatg
    11881 ccaaccatcc atttctgaat taggtttctc gtttagtaac ttcaccagtt cattatcagc
    11941 ttgtggcata ttgtttttga ccaattgaat cggacttgaa gcaatatcac tagcaataat
    12001 tcgaacggct gcaaacacat cactatttct taatgcacga acgcttgtaa agctggtggt
    12061 gtaatcatcc gattggattg ataccacatg ttgtaggaaa ggatctcccg ttgtattttg
    12121 tctcgcttta aaaaatgcca tcttttaacc tcccttctgt tctgtattta gcatgaccgc
    12181 taccccaata agcactatgc ccagcgctaa caagccatag tagacatttg ttagtaaggt
    12241 gatagcaacc aacacacaca ccaatccaag aagtaaaaga acggtatgaa tgtaattaga
    12301 aagaaaattc ttcacttgta taaaattcat tgtctgcttt cgctccttcc gttgtggtga
    12361 agtaatccat tgcaaacaca taggcattga ttaatgcagc tattggatca attttgttac
    12421 tgttctttga tttattaatc tgaatgccgt tgttatccgt ttttaaaata gcgttgttca
    12481 ctgcatgggt gagaatcgta tttttattgt gaataacgtt gccttcataa acttgttctc
    12541 taaaagttcg ggtcggaacg tttagagtaa tcgttccttg tcttacttct aacattgggt
    12601 agttggcttt ttcagcttta ctgataagcg aattagcgtt atacgggtca taacaaatcc
    12661 ccatacattc caaatcattt tcttgaatca aatcttgaat aaactgaaat acttggtcat
    12721 agtccacgat tccactttct aattgagtaa tggaacactc accagctttt tctagttctc
    12781 gataaggcaa accgtcgcgc ttttctttgt cttgaagccc atatttggtg gctacaaagc
    12841 tatgtgaatc acaataaagt tcgccgttat ctagcggtac gatccatgaa acgctagtta
    12901 aatcatctgt tttagataaa tccactccga tataaacggg cttgcctgta atatcttgtg
    12961 gttctacttc ggttgcctgc cagtcttcag caatcatata gctgtcctca ctcgcttgtc
    13021 tccataaatt gaagtttttc accaatacag aattaaggtt attttgtttc aaagcaaggt
    13081 tcacatcatc ttgaatggtt ggaatcatta cttttttgat ttcttcactt tcaaaaatcg
    13141 gattggcttt gatccatttc tcttgatcgt gaatttcttc gggatcgtcc aattcccata
    13201 tcgctataaa gtacctgtcg gcttgttctt cgccttttag cacacgatct aacaataagt
    13261 attcctcgta cataggaacg tttaaatcaa gcccagcggt actaataaca cacaataagc
    13321 cgttcttttg ttgggtcatt cctgacttaa tgacgttgta cgttttacga gtttttgctt
    13381 cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac
    13441 tagccagtgc catagcgaac gaattagagg gcaagtgctg aatcttagag ttcataattt
    13501 tcagttgtgg tcttaaaaac ttactagact tgaccacgtt tctaagtgaa ttagacagca
    13561 tatcatagcc caatttagct tgtttcaaag cgttacttac aaacaatact tgccttgctt
    13621 ctgctggttc ttgttccata atcaacgcat tggcagccat gccacttgcg atatacgttt
    13681 tcccattctt acggctcata ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt
    13741 tagtacgcca gccataaaga ctgcctagaa tgaatttttg aaatcctaac atcttgattg
    13801 ctttaccgtc cgtagatggt agcaactcaa tgaatttaat cgctttggtg gcttgttctt
    13861 catcgaacac ataagggaag ctatctgact tagaacgctc caaatcgctt atatgacgct
    13921 cacacgcttg gataattttc ttgggtgcaa tcagcttacc tgataggact ttctcaatgt
    13981 aatcattcat gaattcatca actctttgaa cgggtcttcg ggttcatcat ttttaacctt
    14041 gttcaaagct agttttgatc gtgattctaa ggtcatacct agtgaggtgg cttgtttttt
    14101 caaatcactc atagcttgtg attgaacccg aaccgctgga ttggctttct ttactccgtt
    14161 ttcgcatgtg ataaacgtac ctgattttct tatctcggct tcggctgttt tcattcgagc
    14221 aaaggctaaa caataggcta ccaatgaacc ataatctgct tcactcaatg gataatcttt
    14281 ttttaatgtt ggtacgatac gattccattc acttctagcg ctcgctgcca tccagtcagg
    14341 gggcgtgata tttattaatg gttgctgctt gaacaattct tctctagcgt ccacacggat
    14401 tgctttttct tcacttgata tattgccttt tgtatcgtgt aatattttag ggtgtcccat
    14461 gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca attaagctca cattccttcg
    14521 tttttcgatc agaatttttc tttatctaat ttatctatat aaatgatttt tccgttctgc
    14581 atttgaatgg tcacttgacc aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta
    14641 tcagtaataa caattgtatt tttcaataaa tctcctactt tcattatata ctttttcact
    14701 tgtgattaca agtgtaatcg ttatttgtaa ttacccttat gccttttcac aacggttatt
    14761 tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca cactatgccc ccttaactca
    14821 aagggtactc ttttctcgtc tttgtctttc attggctgtc ttggaattat ggtgtgcatg
    14881 gcataaagaa cgaaaattag tttcatctaa ccgtctattc caatcatctt ttagttcaat
    14941 tttgtgatct acaacatcag ctttccttat caagccttct tctaggcaat cctcacatac
    15001 tggatgattt aaccgataca agtatgaagc ctttcgccat gttctacttt gatagaattg
    15061 aaaatattta ccttcgcttt gctttctttc ttcacgctta attgccgttt gctttgcttt
    15121 atgcttccca caatacttct gcctataatc aatcaatgtt ttacatccag catgattaca
    15181 ttgtttttta actgccatag attaccaacc aaagcgatct cgttcgtcag aatcatcttg
    15241 ttcccatttt gatttgtatt caatataagg cgaaactatc ttcaagcacg ttccgtcttt
    15301 aaaattaact ttagtgattt tatcttcctc aaaactaata aagtctacat cttcttgagt
    15361 gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta atactttcca tatgcataat
    15421 tattctacct ccaataaatt agttatcaat tcatcatctg ttagtagcaa agccaagaac
    15481 ttatcatgga tgtttaaaaa caaattatcc tcattcgatg taagctctcg ttgtctcgct
    15541 cgatcatata ggttaagcag acaattaaat tcatccatta gctcacctgt atcatcatag
    15601 ttgccactat ctaccaatga tcccactact agcgattgac accacgctac tgactcctgc
    15661 agccaatcag gttcttcttc cattatcttt aatacttctg gatcaaatgt ggaataatct
    15721 tctgttacca atcccacact aaatgccagc gtgttcatta gaaaccgatc catttcatct
    15781 gtatattgaa agttcggtgc tttgtttgtt gaaataatat tgagcattta ctttcctcct
    15841 tgattcgtaa cacgtggtaa cacctacgta acaggtaccc gttaccactc aatcccaacg
    15901 gggacaaggg ttctagcgtt ttggtaacag gtaacgggta aaatccagta acattattcc
    15961 ccagcatatt agctaactat ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca
    16021 aattgctgta tcccttgggg cagtaagggt aaactagtaa cgggctaggt gttactgacc
    16081 cgttactacc cgttactttt tgtactttcc agctcgattt ttattttcat aggcatttga
    16141 cttattgtaa aatcctcgtc gtttatttct ttttggcagt tgcttccgtt cccaaccagg
    16201 aatactgcta ataatcaagg caatcttttt agtttctgca tctgatcgac cattcaataa
    16261 atcctttgct tgtttatcaa aaacagcttc caaaatatca gcggtgagta cgctatcaac
    16321 taaataaaac tcttgttgca tagtagctcc attatagaaa tgctgtaaat ttttctcatt
    16381 acatttactg tcaggatagt tctgataata agaacgtctc gcccacgctg gagcttcttc
    16441 ccatttatac gggatttcca tttccaaata tttagtgatt gcttctttaa ttggattctc
    16501 catcttggca tcctcttgat agtcttttgc aatttctaag acttctttat cttcatcggg
    16561 actaaaatag attcgttgtc ctttatcaaa aagcaccttt gcttcagcta acacttgaag
    16621 aaaatattca tcctttgttt taaacggatt catctttggc ttattcttac aaggcaaagg
    16681 ataaaaacgg cggtttcctg tatcatcttt caaatactgc ccatcattcg acgttccaat
    16741 aaatacacat tgccttgccc aagctatgac attccgacca taaggaggtc taattttatc
    16801 gatcttggca ctgatgaagt tttttatctg ttcaatttta gttttgttca ttgaagataa
    16861 ttctccaagt tcaataatta cattgccttg aagctgcata taatcatctt tcttttcacc
    16921 tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact
    16981 cttaccaatc ccttgcggtc catccaaaac tggaacgatc tcaaacttta ttcctggttt
    17041 atagatacgt gcgactgatc cgactatcca acgttttgtt acttctcgtg tgtaaaggtt
    17101 gtcgtctacc cctaaaaggt caataaacag tgtttccagt ctaggctgtt gatcccattt
    17161 aactttttcg atacgttctt ttactgggtt gaaaacattc tttctagcca catttactac
    17221 tgctttgtgt attttgttgt catcaaaaac tacattgtag ttttcttcaa tatatgccgt
    17281 taaggcatcc tcaaaactgt cttcccattc tccagcggta cgattgatcc aaggagaatc
    17341 ttttaatagg tgaatgtatc cgctaaattc gttataagca attgttccag ctaaattgct
    17401 atcattggtt aaaatcaatt ctgcattcac aacactgtta cttttaatct gatcgtcttt
    17461 attgcatctt aatcgtgaca tccaggtttt caattcaatg atattgtctt cacgctcgct
    17521 ggcttcggga aagtctaaaa tactatctaa tttatgctta tgagccatga gctttccccc
    17581 tcaaatcttt tttaatcatc gatcgaataa tttgattcac ttctttatct tttagcggtg
    17641 gttcattgta cgcatttgct gtataaatca attctgctaa agtttgatta tctacaccgt
    17701 gccatagtaa ccaaccgcat aacttagtta aatgatcgtt tctactacct tcgccagcgc
    17761 cttgaacaat catatctaat ttttttgctg tccctgtttt tgctacgggt cggtattgag
    17821 tctttatttc ttgcggtttt tttagcagta ggttcactag ccaatcggga gctggtgcaa
    17881 tattatttag agatacgttt tcaataggtt catatgctct cccaacactt cttgaaataa
    17941 tcatagttgg cgctattgga actcctttag tgataatatc aatcccgctt aaacttttac
    18001 ctttatcatc acgcccgaag atatcagcaa atgcacctgt ttcttgttgg ataattacac
    18061 catcaggaat tttaaagaag aaatggattc ctttacctgg tgtgatttcc gtataagtag
    18121 aaggaaatgg atcatacatt gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt
    18181 cagaatgttg atctaggtca actaccacca tttgatgatc ttcaagcatt aacccaatat
    18241 ttctagttgc atcttccgtc caccattcat taatttgatt agaatcgtta gttgcatcat
    18301 tccaaccact agagcctttt attggaattt tagttccagg agataaggga taaattgacc
    18361 atcccgactt ttgtaattcc gttgctgctt gataattatt tgccaataaa cctctcctcc
    18421 tttttcaaaa tactggtata aatcattcag acacctccat gagcttcaac tgctcaagtt
    18481 cgtatttcaa cccatcccga acaaattcag caccttttaa ttgattctga tactttaccc
    18541 atgtattaaa tgttttcacc cttgcttctt gcatatcagt aatgtttaac catgcggtta
    18601 aaatctgatt ttttagctcg ttgatttctc gacaaattat tttgtgttct gcatgttttt
    18661 tgagtacata caaaactgtt agtttttcat tgaatgaatt tccaaagtag gttttcccga
    18721 tattattttc tagttcagcg atcccttgat taatttcctc taattcaagg ctatcgagtt
    18781 ggtttaatag ttttgttttg tacatctact tatgccctcc agttcttaat tgatagtcgt
    18841 actcgtcata agcagtgaag agatataacg ctaatcctgc aaagatagct atttttgcaa
    18901 ttaatggtaa taagccgaaa attccgccga ttaaaatgct tcctataatt aagtttcgtt
    18961 tcataatggt tccctccgat tttttaatat ggtataatcg gaacaaagaa tatcgggttt
    19021 tatcccaatt aagttgcctt tatcctgtcc gccaagactg ttatggataa ggctttttct
    19081 ttgttctcga tcatgttaat gctcctaagt tgtctgcaat tcagcagaac gattataaat
    19141 tttctcactc actgaaaact gcaacccata tttttcttta acagtcatta attctactgt
    19201 ctcatctaaa ataggttcac gatcaaccaa catttcagga gtcatatctt tcttttttac
    19261 cattttggaa tgaccataaa tagttgatac agctttattg gctatcacat tcgctttaat
    19321 gtagtctact ggttttggtt cagtaatagc gtggcttaat ttagtcattg cttctttttg
    19381 gtgttctttg tctagcatgc ggaatacttg aaagccttca agtcctgtgg actggcgtag
    19441 ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg gcttcttttt tacggctttt
    19501 gaatgccatg cggtaaatat tttttttcgt tgatgatgtt tacatcttgt gttctgccca
    19561 atgaatcgat gaccttacta gtagtaaccc catctttagg caaagagtga atggctctag
    19621 taacctgttt aagacctaac gcttccgcaa catctttcgc cactgcccac cattcatcat
    19681 taatatcgac aaatcttata atgtgtccgt tccaaatttc agttttcatt caacgtcctc
    19741 cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata
    19801 ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact aaatcgttca agccacgcaa
    19861 tactccgctt accgttggtt cttgtggtag caatttttga tagtccattt gttcgtacaa
    19921 acattcaaac gtttcttcta gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc
    19981 ttctgtaaga attgttgata atactttttc gattgcttta ttttgtgtcg attccattat
    20041 tatttcctcc tattaatccc tttgccccca cggcttgcgt ttatttttgg tattcttcca
    20101 taaagcgatc acattcatct tgtgaaattc ttacgactcc atcaactata atcaacttta
    20161 aaccgttagg aacatatttc tgcatcatag tattgtatga aacgttcata tacttagcag
    20221 cttgtgcctt gttcatatat tttggaaact ggtacttttt ttctaataat gaaagtaacg
    20281 ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc aataatttta aaagctggat
    20341 catcaagcgt gagtttgtca atttttaatt ccatatttat cccccctcgt tagcatacat
    20401 ttctatacaa agattttaaa aaaatgcaag aggctcttga atacatttct atacctctta
    20461 taaactcaat ataccatcat accttttcat ttgcaagaat tttaacatac ttttttgaac
    20521 ttttatgttt cacacatatt taaatcgtga tatagtagag aaaatatatg aatggtggtg
    20581 aaaatgtggc tagaaaaaaa taccccgaag cagtggagac aggacaaaga attagactgg
    20641 ctagaattga aaagggactc tcaatggaac aacttggagg taaattatcc ccaccggcca
    20701 gtaaaggtgc cgttagtaat tgggaaaatg gctataatct tcccaataac accaggctta
    20761 aacaactttc aaaggtatta gatgtatcca ctacctatct attaaacgga tcttatacat
    20821 taagagatat gcatatgatg cccgaaatta agcgaaatgt gcttgaaaag caattgaaaa
    20881 aaactattga aaaaaataac tctataattg attctaatct ttctaatgct atcaataaca
    20941 tcgaagaaga taacctgact gtctcacaga agatttattt aacaaacgca ataaaatatc
    21001 ttaaaacagg atctcgaatt gaatttcctg acgggagtac cccaattgct tatctcgcag
    21061 ctcttctaaa tacgctttta cgatatgact cattaattaa tgattcagaa aaaaatacag
    21121 atgagaaaaa agagtatcaa gaattttatg aaaatgatct tctaaaagag ttcacagctc
    21181 ttgttactga gctaaataaa cactattcaa aataataata aactgcctcc tccaaccgcc
    21241 cccacggctt gctacactat ggagggaaaa ctattatggc aacattcaag caatatgaaa
    21301 ctaaaaaagg taaattttgg ctctatgaag cctatcttgg aatgaacaaa atgactggca
    21361 aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa agaagcccag cttgccttgt
    21421 cacgcttgca agtggactac gataaaaacg gtattaaaaa agcgaacaac gaaacgttta
    21481 aagaagttta tgacttgtgg ctagaaacat ataaaaccac tgtccgagaa gccaccttca
    21541 tgaaaactga aatcaagttt agaaagtgga ttcttccaaa atatggaaat cttcgtgtga
    21601 atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag ctgggctgaa actactgacc
    21661 aatacaaggt acttcattca acttctaagc gtatttttag ctacgctatc aatcttggga
    21721 ttattgagac taatccacta gacaacgtat taatgcctaa aagaaccacc actaagcata
    21781 aagaaaaggt caaagtttac agtaaagaac aactgatcac cttgtttgat tacctaaaca
    21841 gcaaacctga aacttatcga aacgactttg ataaaacgtt actacgcttt ctcttttata
    21901 gcggggttag gatcagtgaa gcccttgcat tgaattggtc agacattgat tttcaagaaa
    21961 atacggtcac catcaataaa acactaagcc aatccaaaaa tggctataaa atcagtgatc
    22021 ctaaaactga ttgtagtgct ggggttcttc cactagatga aagaacaatt tctatattaa
    22081 aaaaatggca gattaatcag cggaaatata tgctgacact tggtgtaact gatccgacta
    22141 tgattttttg tggtatctat aaagaactaa tcacacacca cgctatttat gcaagattga
    22201 ttactatttg tgaaaaagct atggttccat ttctcggtgt gcatgtagca agacacactc
    22261 atgcttcaat gctcttggac gctggagcta ctatggtaga agttcaaact agattacggc
    22321 atacaaaaat tagtcagacg atcgattgtt acggtcactt ggcaaaagaa acgaaagaaa
    22381 agactgttga aaaattagta caacacttaa atattgacta gatggcatac aatatggcat
    22441 acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa ggtttctcct agctttatag
    22501 tctattcttc ttcttccgcc atgaatgtat tgaatacttc ttcaatcatg tcccattctg
    22561 cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg atgtatgcat
    22621 aagcttgtaa ttctacatct tcatcttctg aaacaccagc aggatataaa agaacatagt
    22681 tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat ttcatataat gtttcatttc
    22741 cttggtcatc caccaatgtg atgtgttcgt gtccttcatg gtcgtgggaa tggttatgtt
    22801 ctgtcataag attacctctt ttcttttatt attaaaataa ttgcttacct gagccgtcta
    22861 aataattttg taagatcatc acagctgcta atttatcgat gacttttttt cgtttggctc
    22921 ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt aatcgttcat
    22981 cttgataatc aactggcaag gagaatagtt ctttgatctt ttctccgtat gacatggaag
    23041 cttctgccct tggtccgatg gaattgttca tatttttagg taatccaact acaaaccggt
    23101 ctacttcata ttgtgctact aattccttca accgatcaaa gccaaactct tcttctgctt
    23161 cattgatccg aatgatctct actccttgag ctgtccagcc aaaggggtca ctgatagcaa
    23221 cacctactgt cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt
    23281 tgagaggtag tccttgacaa ttacttccat gatctcatcg cgttcgtgac ggcgaatcaa
    23341 atttcgagca tcacgataac gaggaatgta ggccgggtct ccagaaagca agtagccgac
    23401 aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt
    23461 ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa
    23521 acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct acaaccttat
    23581 tttactcgaa gaaagggaaa actacaaaca aatattccaa acttccgaat aaattaagaa
    23641 acactatttg tatgtttttt tccttttttc tgtagcttta tcaaaagtac tcttttcata
    23701 aagtatacgc tatatttctt tgaatgtatg tttttacaca attttttcat aatattcata
    23761 aaaaattcga atagctgcta ctaatttttt gtttttgttg cttgtcctgt ctcataattg
    23821 atcgtgtagc catcttgaac attgtagata aagacattga attcaatttt tttgtcttca
    23881 atgctttctg cttccaattg cactcccctg gctaccagtt cattcccctc aaaatgagga
    23941 gtgacacggt agcgtacatg atgatttgtt tctttgatat atgccgcaat atcattttcg
    24001 tgagcaagca tgtatggagc attcaacgaa cgtgtccctg tcatcaagtt tttaatgttg
    24061 ttattttgac cagtcagctg gaaaccaatt aaatggctgc gattatataa ccattgcccg
    24121 tcacttaatt tcttttgttt ccaacctgtc gggtcaatat acaaaggctc tctcttttca
    24181 gtagggaaca aatcttttcc aagcatagca ttggcttgtc ctactctgtt caatttatca
    24241 atatttgaat agctttccca tgttcctttg gaaaggctaa gatcctcttt tgtaaattcc
    24301 ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg cacatccgct
    24361 acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa tctgctgaaa gatatctgaa
    24421 tctttttgtg tggtggaaac accgaatgcc cctgcaatca cagcagcgac gatccccacg
    24481 atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagct ccctaaatct
    24541 taacatatgt tcccctataa ataaatacta gaaagaaata gaaacagcaa aaaataaaac
    24601 ctgttttaag atggcttaaa aatacaaaaa agagacggat tcctcgtctc taatcagtca
    24661 ccactctatc tgtaaggtat ttattattat tcaaaaattt tcttcatact caagacaata
    24721 cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc ttgcatctgc ggtaataagt
    24781 ccatccaagt taaaaacata agaagttgtt tttgcttggc tgttcttatt acttgttgca
    24841 gagcagcttt ttcacaacct attttaagcg ggtgacggga atcgaacccg cgacatcagc
    24901 ttgggaagct gggattttac cactaaacta cacccgcaat acaaaaacag tatacgcctc
    24961 ttttattcct tcctcaagtt tttttattct ttacgcttct ggtatgtttt gtagtaaaat
    25021 aaaactaatc aatcaattat tggagaagat gctatgacgg gaaaagaatt agatctgttg
    25081 ctggacactt gtttactcgc tgggaaaatc atgatggaaa gcaatgcgga gatgtatcgg
    25141 gtagaagata cgatgagtcg aattgcttta gcttctggaa atttccgttt ggtcagctat
    25201 gtcactcaaa ccggtctctt tattggtctt gatcgtacct cgaccatccg aatggaacaa
    25261 attaccaacc gttcgattaa tttagaaaaa gtagtaaaag taaatgatct ttcacgtaaa
    25321 tatgttgctt cagagctaac attagaagaa ctttactgcg aactgcaagc aatcgaccga
    25381 gaccgttctt tctttcctgt ctggttgcaa atcgttagcg cggcagtgat cagtggaaca
    25441 attatggtgt tatttggcgg aaagatgacc gatttacctg tgactttagt cattggagga
    25501 ttgggatacg cagcttatct atatagtttg aaatttttta gaatcaaatt tttatcggaa
    25561 tttttatctt ctttattgat tggcatagcc gctatctaca gcgtacgttt tggcttagga
    25621 agcaatcagg atctaatcat tatcggatgt gtgatgcctt tagttcctgg agttcaaatt
    25681 acgaacgcta tccgtgattt gctagctgga cattatgtct caggagtttc tcgcggaaca
    25741 gaagcgatga tgactgctac gatgatcggt tttgcgattg cttttatttt ccagcttttt
    25801 tattaggaaa ggaacacaag tatggggacg atatttattc agttttcttt tagtttttta
    25861 gcgactgcgg catttgccgt tattacaaat gtacctaggc gttcattgat ttgttgtggt
    25921 ctttctggtg cttttggctg gatgatctac tggatctgtg ttcaaatagg cggatctgct
    25981 gcttttggtt cattgcttgg ttctttagga gtagcattca tcagcgatct tttctctaaa
    26041 cgtttgaaaa tgcccgtaac gattttcaat attcccggga tggttcctct agtccctggt
    26101 ggtttagctt atcaagcggt tcgaaatctc gttactgaat cctatcgaga agcggctcat
    26161 tatgcggtcc aagcaattat gattgctgga gcaatcgctt taggtcttgt attatcagaa
    26221 gtctttaacc ataacatccg taatttcaaa gtaaagcgtg acgcaatcat gatgaaacga
    26281 aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa aggttgtgaa agagacgttc
    26341 agccttgagc gatgacaaaa tatctcacaa acattgcgac aagtaaccgc aggagcaata
    26401 agaagtaatt atgagtatta aaaaaagttt tgtaaaaata gcttctcaca tttttacata
    26461 aaattgactt aatactgaga aactgacttt tgaacaccct ctatctgtaa aaaaggggct
    26521 gcgacatgat ttttgtcacg gcccctcttc tttattcaaa ttttttccaa aacgttcgtt
    26581 gataataaac gatccataga gcggaaagta ttagaactaa accgatcaca ggaatattca
    26641 ctaaccaaaa tggataaaac aaagccaaca ttccgtataa gacgataaga ataatactga
    26701 tgatcaatga acccatcata gttgcgacta aacccaaact tcctgaacct ctgttaaata
    26761 attggttaat attggtccag ttcagcagca gcaaccgata atctctagcg aaataacgta
    26821 gtgacaataa gtagcagccg aataaagaac caaatagcaa gctagggata aaaaacaaag
    26881 gcagctgaaa agacagtccg cttaataatg cgatcccacc agttaaaaaa ctctgcaata
    26941 tccatccgaa tctgaacttt tcttttaaat attggttcat agagattggc aaagaacgga
    27001 taaacagaaa attttcttga tccaaagaaa taagattgct gataaaagaa gtctgattaa
    27061 ctgttaagaa agccaatgcg atccctacca ggaaaacaac accgattaac ttattatcta
    27121 aatcagttaa ttttatttcg cctcctaggg caaaagcaag aataaaaatg attggagtta
    27181 acaaggaaga agaaagaact tgcataatca aattaggttc ttttactaat tgagcattgt
    27241 aactaaataa aagctgatgc acattttggt tctttttatg ggttcttttt attttgaact
    27301 cactgtttga atcagtcagt tgctctgtaa gctttggtac tatcagctgt tttacagcaa
    27361 ggaaaaatcc cagagccgtg atagctagta ttccccaacc caataaacct tgttgagaaa
    27421 gtggctgact ggcagcatca tagaatggca ataatatcga aatcggatgc cgatctgtta
    27481 actcattttc aatagtcccc gtttgatgat tcatccacat gattccagca aatgcgatca
    27541 gcatcgagct gccaagcaag atactagaaa ctgtttttct atgtttttta aacactgctg
    27601 ttcgagccaa accaaaaaca atcaaactag ccagacaaaa aagaataatc aaaaatagag
    27661 aaaacaaaag gattgagata atgatagtaa gcaaaatccc tagatcagct cgccatccgg
    27721 ttaaaataaa catgactaga agcggaaaaa caaaaggaat gatggtcaaa gcaactacca
    27781 aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag aggcaaaaat acttgcaggt
    27841 catttccttc aaaaaagata ttataaatga ctgatatact ttgagttaaa cctaacatac
    27901 caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt aacaaagttc atagcaaaca
    27961 tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa aagattttga gacaaaagat
    28021 agatagttaa tcgctttccc ttttttcctt tctctcttgc tttattcgtg agctgtggat
    28081 taacatataa aagattaact cgaattaatt ctaataattg tcgcttatac atacggtcca
    28141 tctcctgtcg cttgccttcc agccattttc aaataaatct tttctagcgt ttcatcaggt
    28201 gagccattca ataattcact aactgccccc tgataaatca attcaccttt cttcaaaata
    28261 gccagtttat cgcataactg ttgtgcggta tccaatacat gtgtagaaaa aatgactgtt
    28321 tttccttttg cggcatgagc cttcatcatt tctttcaaat caaatgctgc ttgaggatca
    28381 agtccttgca aaggttcatc caatacccaa atatctggat ctggcagcaa cgtgccgatc
    28441 aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga ttgtttcatt ttgatgacta
    28501 tacatatcaa aaagcgctgt caattctgct aaacgctttt cttttttttg tgtgtctaat
    28561 tcataggcag ctgaaatcaa gtcccaatat tctccagccg tcaattgtaa aaaaatatct
    28621 ggggaatcag gcacataacc gattttttgt ttgatactca atcgattttc cgtcaatttc
    28681 attccatcta ctaaaatcgt tcccgatgat ggttcaataa tactaactaa acttttaatc
    28741 gtagttgatt ttccggcacc attatgtcct aaaaatccaa aaatttcccc ttgtcttatc
    28801 gagagagaca agtcttttaa tgcttgtttt ttcccgtaac ttttacttac attgatgaat
    28861 tcaatcattt ctgtactcct tttgtttgtt attacttttt attgtaccct tcgttattgg
    28921 tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata gaaacgacta ggctgctact
    28981 tgggttgttc tcttctacag gaaaacactc ttctaacagg taagatatga gtctttgttt
    29041 tctttaaaat gattcatttt ctatcttttc ttgcataaaa ttctttagtt tatcaatact
    29101 tctctttccc atctctagtt gttctccatt tgcaaatgtt actatttcgt ttactctgct
    29161 aatagatgta atgttttcag tattaatgat ataagctttt aaatctttac ataaatactc
    29221 atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct cttcatcttt
    29281 caatactagg cctattttat ttttataacc tggcatgctc attagataaa ggatttcatc
    29341 gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt ttttcgtagc
    29401 atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc tttttttatt
    29461 tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc gattaataac
    29521 agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt cattcttttc
    29581 tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga tgtctaggaa
    29641 aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt gtataacgtt
    29701 caatccctga acgatctcaa atttccaagg acttaataaa attctggatt cttccaaaat
    29761 ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac gcgttaccat
    29821 aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact tttcaacata
    29881 catgttagga ataaattgta attctcttca caatcttttc caacaccttt gaaaccgata
    29941 tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat acatcatgca
    30001 aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt ttatttattt
    30061 tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact ttctcagcgt
    30121 ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct gattggatgg
    30181 ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc atatgcgtca
    30241 acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg aacaagaaga
    30301 tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg atttttttgc
    30361 caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag ttttcatctg
    30421 tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt tggtctaagt
    30481 agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc tgcatctccg
    30541 gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct acgatcgtag
    30601 tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct agcagtacat
    30661 aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc tcgtgtgcct
    30721 tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc aaaagagcag
    30781 ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat tgatcgagtt
    30841 tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc aaaaaccgca
    30901 gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc aatcagtccg
    30961 atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag atttttgata
    31021 tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa cagacttaac
    31081 aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa ggccaataac
    31141 agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat tctagttggt
    31201 aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg acctgtatca
    31261 aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag ctgatttttt
    31321 ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt ttctgctact
    31381 ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag ccagacacca
    31441 atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg ccaagaattg
    31501 ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc agtcagcata
    31561 aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac gatcggtacg
    31621 atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa aatagagaag
    31681 atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac ttgtaaggcg
    31741 cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat cgataagaaa
    31801 atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt cctccttttt
    31861 atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca agttgctctg
    31921 taaattttta cacaactctt attttttacc aaagcacttc aattgacttt ttctgttatt
    31981 cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa taaaaatgaa
    32041 acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg ttgccgtcat
    32101 ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac tagaggaact
    32161 tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac cagaagtagc
    32221 tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag gaaaagaagt
    32281 ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag atgaactgac
    32341 agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat cgttgtccaa
    32401 acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat cgctttttat
    32461 ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa atagatcaag
    32521 gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg agcattcaat
    32581 cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg attgactact
    32641 tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg gatcttgatt
    32701 tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg cccaacgatt
    32761 tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg gaatttgtat
    32821 tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt caattgatcg
    32881 tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc atacccacgt
    32941 tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc tacttgaacg
    33001 gcttcactta attcagttga agacactttt ttctttccag cattatttaa aatccttaaa
    33061 tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg atctttcacg
    33121 aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag cagtatttat
    33181 aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat tcatttattt
    33241 atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa ttgaaaaata
    33301 gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata ttattacaag
    33361 ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata catttagaaa
    33421 tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa tcgactttat
    33481 taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa aataaaacag
    33541 cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca gtttggaacg
    33601 agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc gagctagaga
    33661 ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta aaagaatacg
    33721 accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat gaaattcgtt
    33781 ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc gatactttat
    33841 caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac cctgacattt
    33901 tgattctaga tgaaccaacg aaccatttag atatcgagac actttcctgg cttgagaatt
    33961 atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat tttttagata
    34021 aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac aaaggtaact
    34081 attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag gcttatgaaa
    34141 agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt gttcgcgcct
    34201 ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac cggctggatc
    34261 gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag ccttctggaa
    34321 atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc ttatctgaac
    34381 caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg aatggtatcg
    34441 ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt ggaaaagaaa
    34501 cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat ctgcatacaa
    34561 acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa aaagagatcc
    34621 gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca atccctctgc
    34681 taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac aaagaaaatt
    34741 ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa gtattagaaa
    34801 atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga tatttcatca
    34861 atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc tatctaggag
    34921 actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa ttgttggcac
    34981 aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac caaagcaaag
    35041 aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa gaagaaatgg
    35101 cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca gaaaacctgg
    35161 aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga caagatcaag
    35221 aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat gaaaattaac
    35281 gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt attttatcaa
    35341 acaaaaattc catatttacc tttttcacca ataagaagac caattactag tcattctcac
    35401 taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc cattattttt
    35461 gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg tagcatatct
    35521 ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct tttctgtttt
    35581 gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca ccatactaga
    35641 tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac aaatggtatc
    35701 ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa aatatttttg
    35761 ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt ggcctctagc
    35821 ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt ttccatcttt
    35881 gacatccata taaaatgtat gataagttga accatcttct cttactaaat aatctgctgc
    35941 attttgaata tgagcctgag cagctttttt atatttttga tttccagttt cttcactcgc
    36001 ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt gttgtgaagg
    36061 atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac gctgaagtaa
    36121 tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta ttttataatc
    36181 tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga taatttgaca
    36241 gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa ataaaggatc
    36301 acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg tccaaccaat
    36361 attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt gatactgact
    36421 gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga tctcattatt
    36481 ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg gatgtattat
    36541 ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa ttttagtctt
    36601 tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc aattttggga
    36661 cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac tcctgttccg
    36721 agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt gttttcccat
    36781 gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt aatagtatcg
    36841 tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt ttccttgtct
    36901 tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc tgtctcatat
    36961 agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc ttcaaaagga
    37021 ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc aagtacctct
    37081 ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata ggttttgatg
    37141 ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc tagcattgaa
    37201 tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc aaattgacta
    37261 gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg ccctaattgt
    37321 cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt ggcttctgaa
    37381 agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca atactcatgg
    37441 tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga taacgtacta
    37501 ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag gatacctttt
    37561 tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa ccccttgatt
    37621 tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa gcttgaaact
    37681 gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc cgcaaaaaaa
    37741 tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct aatgggcttt
    37801 tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg attataataa
    37861 tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca attttctata
    37921 ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt ttggataatt
    37981 gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata ttcaaaaatt
    38041 tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt ccataataat
    38101 tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg ctggtctctt
    38161 tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt tattcctgat
    38221 agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc tccccataaa
    38281 acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc ttgatgtata
    38341 taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt tatcttttta
    38401 aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt cattgtattt
    38461 tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt aagccttcaa
    38521 taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta tggtatgctt
    38581 ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag tgcttttctg
    38641 tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca gaggaaacat
    38701 atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta tcaatattcg
    38761 aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt ttctgaataa
    38821 aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg taacgataac
    38881 ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa ttctcattgt
    38941 atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat tctgcgatat
    39001 gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca tgctgccgag
    39061 caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt aatggtaaat
    39121 tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct actaaatagt
    39181 tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct agttgtcgac
    39241 tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat aaaaaaccta
    39301 catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt tcttgttgta
    39361 ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat aaaataccac
    39421 tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct gtatattttc
    39481 cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca tctatctttt
    39541 cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc atggatatcc
    39601 cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta gatggttaac
    39661 attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa tttagactgc
    39721 gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag cataattgcg
    39781 ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat taagggaacc
    39841 gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt ccaacttccc
    39901 ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac agcgattttc
    39961 gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag tgctgtagga
    40021 aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat cgaagggatg
    40081 atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa taaaggaatc
    40141 aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa cagtttattc
    40201 cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc tgtagctaaa
    40261 actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata attatatctc
    40321 gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt gattccgcca
    40381 cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata tggcatcaaa
    40441 aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg tatcttttgt
    40501 ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa cagatacaaa
    40561 caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa aacttgtttt
    40621 atataaattg actcctacga ccaaagctga attttctggt ccattggaag tcccaaagaa
    40681 tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg tggccattaa
    40741 aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt tcgatgctcc
    40801 atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag ctaaaaataa
    40861 aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata ttcgtatcca
    40921 aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa atccattgaa
    40981 taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg ttgatgtaat
    41041 ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg ctgaattcaa
    41101 taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg ccatgataat
    41161 caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta gataattgtc
    41221 aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa acgagataat
    41281 gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca atccataagc
    41341 acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg tcttgcttgg
    41401 taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca gttaatttta
    41461 cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca aaatcacctg
    41521 tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa taagtggctg
    41581 gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag tatttttgtc
    41641 ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat gagttagatg
    41701 tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc ataaatgtaa
    41761 gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa cttgatcctc
    41821 ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga gccatgcgcc
    41881 atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag ccgccagcaa
    41941 caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag aaatgtacaa
    42001 tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct ttagtattca
    42061 gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc atgttgataa
    42121 acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt ttttctttta
    42181 atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa tctacaccta
    42241 tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca ctagcccaag
    42301 gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga tcataaaact
    42361 gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca tcttgaaatt
    42421 tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc atatcttcgt
    42481 gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct acattcacaa
    42541 tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca ttttcaacat
    42601 agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt tttatcattg
    42661 gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt aagctgaata
    42721 atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg tctatccctt
    42781 ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaa
//
LOCUS       DADJYG010000011        33884 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.011, whole genome shotgun sequence.
ACCESSION   DADJYG010000011 DADJYG010000000
VERSION     DADJYG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.011"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..958
                     /locus_tag="JZ382_000500"
     CDS             <1..958
                     /locus_tag="JZ382_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAY2426708.1"
                     /translation="KDSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLN
                     CYQGKVKTILNELETYNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDD
                     DLSETIDKSTSSRNAEIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYY
                     INKSLDTKRHLKDTETDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEE
                     AQQTVTTIHNAKHKAEELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNL
                     QNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVSTENWLE"
     gene            1794..2126
                     /locus_tag="JZ382_000501"
     CDS             1794..2126
                     /locus_tag="JZ382_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAY2426709.1"
                     /translation="MSYTGILSFEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            2717..3046
                     /locus_tag="JZ382_000502"
     CDS             2717..3046
                     /locus_tag="JZ382_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426710.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            3033..3374
                     /locus_tag="JZ382_000503"
     CDS             3033..3374
                     /locus_tag="JZ382_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAY2426711.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3488..3727
                     /locus_tag="JZ382_000504"
     CDS             3488..3727
                     /locus_tag="JZ382_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426712.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            3794..4033
                     /locus_tag="JZ382_000505"
     CDS             3794..4033
                     /locus_tag="JZ382_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426713.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            4119..4790
                     /locus_tag="JZ382_000506"
     CDS             4119..4790
                     /locus_tag="JZ382_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAY2426714.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVLEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4843..6819
                     /locus_tag="JZ382_000507"
     CDS             4843..6819
                     /locus_tag="JZ382_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAY2426715.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            6832..7575
                     /locus_tag="JZ382_000508"
     CDS             6832..7575
                     /locus_tag="JZ382_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAY2426716.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVIYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7591..8364
                     /locus_tag="JZ382_000509"
     CDS             7591..8364
                     /locus_tag="JZ382_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426717.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            8653..10764
                     /locus_tag="JZ382_000510"
     CDS             8653..10764
                     /locus_tag="JZ382_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAY2426718.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(10802..12859)
                     /locus_tag="JZ382_000511"
     CDS             complement(10802..12859)
                     /locus_tag="JZ382_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY2426719.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(12973..13185)
                     /locus_tag="JZ382_000512"
     CDS             complement(12973..13185)
                     /locus_tag="JZ382_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426720.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            13372..13839
                     /locus_tag="JZ382_000513"
     CDS             13372..13839
                     /locus_tag="JZ382_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426721.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            13836..14078
                     /locus_tag="JZ382_000514"
     CDS             13836..14078
                     /locus_tag="JZ382_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426722.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14096..14398
                     /locus_tag="JZ382_000515"
     CDS             14096..14398
                     /locus_tag="JZ382_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426723.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            14470..16524
                     /locus_tag="JZ382_000516"
     CDS             14470..16524
                     /locus_tag="JZ382_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426724.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            16524..19136
                     /locus_tag="JZ382_000517"
     CDS             16524..19136
                     /locus_tag="JZ382_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAY2426725.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            19133..21652
                     /locus_tag="JZ382_000518"
     CDS             19133..21652
                     /locus_tag="JZ382_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426726.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAAEMADNDKSGLSDLQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLSKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            21704..22036
                     /locus_tag="JZ382_000519"
     CDS             21704..22036
                     /locus_tag="JZ382_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426727.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22037..22630
                     /locus_tag="JZ382_000520"
     CDS             22037..22630
                     /locus_tag="JZ382_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426728.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22793..24817
                     /locus_tag="JZ382_000521"
     CDS             22793..24817
                     /locus_tag="JZ382_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY2426729.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIIVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24817..26022
                     /locus_tag="JZ382_000522"
     CDS             24817..26022
                     /locus_tag="JZ382_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY2426730.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            26038..26682
                     /locus_tag="JZ382_000523"
     CDS             26038..26682
                     /locus_tag="JZ382_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426731.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIEQLKQQDGTKAKEEVTEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26693..27499
                     /locus_tag="JZ382_000524"
     CDS             26693..27499
                     /locus_tag="JZ382_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426732.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            27521..27751
                     /locus_tag="JZ382_000525"
     CDS             27521..27751
                     /locus_tag="JZ382_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426733.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28297..28572
                     /locus_tag="JZ382_000526"
     CDS             28297..28572
                     /locus_tag="JZ382_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426734.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28614..29036
                     /locus_tag="JZ382_000527"
     CDS             28614..29036
                     /locus_tag="JZ382_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426735.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            29055..30647
                     /locus_tag="JZ382_000528"
     CDS             29055..30647
                     /locus_tag="JZ382_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426736.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30659..30994
                     /locus_tag="JZ382_000529"
     CDS             30659..30994
                     /locus_tag="JZ382_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426737.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            31141..31335
                     /locus_tag="JZ382_000530"
     CDS             31141..31335
                     /locus_tag="JZ382_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426738.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31367..33739
                     /locus_tag="JZ382_000531"
     CDS             31367..33739
                     /locus_tag="JZ382_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426739.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT        12479 a         5207 c         6850 g         9348 t
ORIGIN      
        1 gaaagacagc tcaaaaatag cttatatgct ttttaaaaat gaatttcgta gagctttgca
       61 tgaaaaatgg atcgacgatg atggactatt atatttggaa tttacacaag ccgatctaat
      121 ggataaatta aattgctatc aaggcaaagt aaaaaccatt ttaaacgagt tagaaacata
      181 taatttgatt tctgtcgtaa aaggagaatt taacccaaaa acgaaaaaaa atgaaaaaaa
      241 taaatactat ttacttcaac ctgaaataag tgccgatgat ctttttattc aaaattcttt
      301 cgatgacgat ttaagcgaaa ctattgataa atcaacctct tctcggaatg cggaaatcgc
      361 acgtcgagag gaaactgcta aaacccttga taatactgga aatgcggaaa tcgcacgtcg
      421 agaaaaaaca gctgaaaccc ttgataacac tggaaatgcg gaaatcgcac aagattatta
      481 tattaataaa tctttagaca ctaaaagaca cttaaaagac actgaaacag accaattaca
      541 aaatcaagtt ttgctagata acttcgttga aatcatgaaa gaagacagca taaacacatt
      601 tgtaccagaa aaggtattaa gcttgataaa aacattttct aacacttacg aagaagccca
      661 acaaactgtc accacaatac acaacgcaaa acataaagcc gaggaacttg agaagacggt
      721 agttgtgtat gaagaacttg aatcttatgg aattgatgct gacaaaggat tatatatgac
      781 tttactgaaa gcttatcaaa aacaaaaaac tgaaaaagta aacaatttgc agaatctaat
      841 tttcgtttat gtaaaaaatt ggtttgtcga aaaagctatt gcggcaaagc ttgcaaacga
      901 acaaagagaa acatttaacg aattaccaac agttagtacg gaaaattggt tagagtagtt
      961 gataaaaagc gatcactata aaaatgatca attttaaatt agaacttttt tatgatttta
     1021 attttattac aaatgttaca aataaatagt tattattccg gagtttttag attaaaagtt
     1081 tatgaaaaca atcacaaacg taggttttaa agtcataatc gttagatatt ttagttattt
     1141 ttcattcaaa aaaataggga actagtgttt gcatatgtgc ttttttgagt tataataatt
     1201 catgaagaga ggtaattcac ttatttcccc aagctgaaaa gcctgatatt acttcttttc
     1261 aggcagtgct gctaatattc aattacactt ttttgtatat ttttagttgt tttaaattta
     1321 attgtctgat atgcttgtga tgaattgatt ttcgttctgc acttttggac gataaattgt
     1381 gataattgaa aatacaaaaa aaagaaaggc ccctgtaagg ctcgggagac tggtaatctc
     1441 cgtcagtgtc ctacaggaaa gcattaagat gacgccttaa tgcgtgccct tcagtgttac
     1501 ggtattaccc gcaacggact cagtataaca gatattcaaa agtgatacca ctttttttgg
     1561 ctctattaag gttaaaaata cgtgatattg taatgaatct atgcctaagc ataatgttat
     1621 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     1681 ttgaatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     1741 aataacaaga aaaacttatt aaaaatattt aaagaaagtg agaggattac tttatgtcat
     1801 atacaggaat tttgagtttc gaagatattt gtcattacgg taaacggtgt accgctacag
     1861 agaaaatcac gaagaaacta tcaactggcc aaaataaaac cgttgtgcag tgtaagaaat
     1921 atataatcca gaaagacaaa gtctcagaag aaatgattta ttacattggg aaacaaaagc
     1981 aaataatttt aaaggatcct atccctttaa aagagctata tccaaccatc aaacatgttt
     2041 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     2101 aagggatggg gcgtttgatc agctgaattg atcattctca taacttgtat tttatcgttt
     2161 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagaatcat tagaaaaata
     2221 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gtttttacga
     2281 ttggtaacgt aaaaaattgg tcaatcgagt aaatgccctt gtttggatag tggtgaaatg
     2341 aatttgtgta aataatatgc acagagtaaa caagagggaa tttagaagtt agacaagtta
     2401 gaatagcgcc tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc
     2461 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     2521 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     2581 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgatttatt ttatatattt
     2641 ttgagtgaac gaaaaacgat aaagatattc gttatgaagt aaaactaatt tagtagatag
     2701 caaaggtggt agaggaatga ttcaaaaatt tgcgaaaatg agtaaaactg catggattga
     2761 aaagcttgaa aatgacttga gtgtcatgcg attagctcca aagaaattca aacgagagat
     2821 tgcagaaaaa gaagcaattt tgtcggtatt gaatgcggta gacgataaat gtccagtaaa
     2881 aattagtttt tcagatatag caacaattat cgaaggtatt tattatggtg aagttgatga
     2941 agagatatct tctacaaaag atttattaga ttttttggat aaaaacaaag atacctttat
     3001 agtactaact gattatgagg aggttacaac aaatgaaaaa ttatgatccg aatatacgat
     3061 tgggcacaca tacgattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt
     3121 ttagaagagg cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat
     3181 acgatcaaaa attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag
     3241 gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag
     3301 atacttggtc atatttgagt gattatattg tcagcgttga aatcattgaa tttgtagcgg
     3361 ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac
     3421 gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaattta tttaggagga
     3481 aaattagatg gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag
     3541 gtatgtacag ctgttagctc gtggttattc acctaaagat gtattcaaat ccctgtctaa
     3601 aggaacagat atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa
     3661 tggaatcaag gatttgaaac cagcacggcc agtaataaaa cgaaagaaaa agataaccca
     3721 agactgaagt gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacaaggaa
     3781 ggtaggaaaa ctcatgaatt atgaagaaat cgaaaacaga aaaaaagtgt cgaaagaaat
     3841 ggaagaaaag ttgctaaaaa cgatgaaaca aaaacacctt aaaagattat ctgtcatgca
     3901 atatatcaat gatatgcaga taacaggaaa agagaaagct tgtttgttag gttcaatgaa
     3961 aaactttgaa cagttgagac gaacttatgt gaaaaaaagt tctaattgtc aactattgtt
     4021 agaagtctca tgacattcat taatagaatt catttttaaa actttttcta tggaaatgag
     4081 aaaaagattt tttgttttgt ctgaaagggg gtagctggat gagaaaatgg ttatttggtt
     4141 ttttaggagt cgctttaatc gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac
     4201 atgaaggaaa ggtgtttaag caaaatatag aaaaaaagat gcccgtagat caaatcaatg
     4261 aacatgcaaa gagttataag gaggatgcaa cgaacgtcaa taatgatatg tctttggggc
     4321 aaatgttgtc catacaaaaa gaagcaatcg aaatgggtgt caataaacaa gcctttgcac
     4381 aaattcagat acctgcactt ggtttagcat tgccaatttt taaaggggca aatcaataca
     4441 ctttgagtct tggagcggct acttattttt atgaagatgc cgaaatgggc aaaggaaact
     4501 atgtcctagc aggtcataac atggaaatgc caggtgtttt attttctgat attcaaaagt
     4561 tgtcattagg ggaggtgatg gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa
     4621 cgcgtaaatt tatcgtactt gagtacttta aattaatcga tggcgttcct gaagagaact
     4681 ctttcttgtc tttaccaaag aaaggagaga aacccttatt aacgttgttt acttgtgttt
     4741 atacgtcaca aggaaaagaa agatacgtgg tacagggaga gctacaataa caaggtttta
     4801 atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa aaatggcaat aaatcggtat
     4861 gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca ttgcaagctg ctttcagggg
     4921 tatcaagtac acgcagaaga tgtcacgcaa aaaacgccac cagagaaggt caatatcacg
     4981 gttcataaat taatgtacga tcaaggaacg caattgaatg ttgatattga cgggatcaaa
     5041 aacgatggtt atacgcacga tcaatatcct acaggtgtaa cgaaatacaa caaggcagat
     5101 tatggtgatg ttgagtttac gttaggaaat attacggatc aagtattacc gagggaagat
     5161 tctgatctaa ctaacgcgaa agtggacgaa atcgtgaaag acgtagaaga caagggatct
     5221 aacagtgagt acgtcaagaa tgcaaccaac aaaattactt cagctgtaga tgaaaatggt
     5281 gagatcactt ttgctgatca accagcctat gtcaattcca aagggaatgt atatgtggtt
     5341 tatgaaagta aatccgctgc tggattagtc acacaaaaag caaaaccaat ggttgtgatt
     5401 gcgccaatga ctgataatac aggttcaggt tttttaaaag acattcactt atatcctaaa
     5461 aatattgttt ctaaattgag ttttgaatta acgaaatttg gtgacgatgg cacagctcaa
     5521 agcaaacaaa cacctttaaa aggggcaaaa tttgagttat acaagggaga acctggaaaa
     5581 ggaacaaaat taggagatct tgtatcggat gatcaaggaa agctgactgc tactgatttg
     5641 acgttaggaa aatactattt tgtggaagtt ccttcagaag ttgtagtggg atcagataaa
     5701 gaaccaacag cagatcaata tttattaggc gcagacgctc gcaacgatgc acacaataaa
     5761 ttaaccttcg aaattacgaa tgatggcgtg acaagtgact taaaagcttc ctatgtaaat
     5821 tacaaagctc ctgtaatcga taagacagta atcaatggaa caggggccga acattctttc
     5881 caaattggtg acgcagtgaa ttatcaagga acgattcata tccctacaga tattgcaggt
     5941 ggaacagatg ggattacagt aaatggtgtg aagagtgaaa cttctcctta cagcgtattt
     6001 aagtggggag atacagcagg taaaggactt tcttatgtcg ctgctaaagc aaatatcaaa
     6061 gtaacaaata aagatggatc agtggtatta aaagaaaata cagattataa gatccaaaat
     6121 tcagaaaatg gctttgtgat cgattttatc gtgaataatg gccaagtaag tgatactgtc
     6181 gcaagtttac acggacaaga tattcaactg acttataaca tgtttgtgaa tgatagtgct
     6241 gcaatcgcaa atccattaac aaatagcgtg gatttcgttt atagcaacaa tccattcaat
     6301 caggaagaac accatgaaaa aacgaaagcc gacgctgtga cttatggcgc aaaattctta
     6361 aaagctgatg gcggactatt tggaacagga atcaaagcaa caccgcttga aggcgcagaa
     6421 tttgctgtta aaaacgcaga aggaaaatat tatggtggat tagttgatac ggataaagac
     6481 ggtgtcaaag aagcagtatg ggtagacgat gtagcaaatg cagcgatcct aaaatcagat
     6541 aaagaaggtc actttgaaat cactggttta actgaaggtg aatattcact ggaagaaaca
     6601 aaagcaccaa ctggttacca aaaattaaca gaagatattg catttaaagt aaacaaaaac
     6661 tcatttaaag aggaaaatcg aatcactgtt aaaaataatc aaaaagcagc tattccatta
     6721 acaggtagta atggtttcca aacgtatgta ttgatctctt gcttgttgct aggagcaggg
     6781 gcactttctg cagttgttta ctttaagaag aaagcctaga aggatcagct aatgattatt
     6841 cgtaagcccc caaaaagaag agtcatgcac aaactgatga tatttttttg gttgctgtcg
     6901 gcagtagcag tctatttaat tccatctgtg gaaacaaatg tagcgaaatt tcaacaacag
     6961 caaagaattt tagccattga aaatcttgaa aaaacagatt cttctttgga tgttgcgaaa
     7021 atagagttag gcgatcctgt tggtgttctt acaattccaa gtatttcttt aaaactacca
     7081 atttatgacg gaacgtccga caaaatttta gaaaatggtg taggtattac ggaaggaact
     7141 ggtgatatta caggaggaaa cggtaaaaat cctttaatcg caggtcatag tggactttat
     7201 aaagataatc tttttgatga tttaccttca gtaaaaaaag gagaaaagtt ttatatcaaa
     7261 gttgatggtg agcaacacgc ttatcaaatc gatcggattg aggaagtgca aaaagacgaa
     7321 ctacaaagaa actttgtcat ttacttagaa ccaaatccca atgaagatcg agtaacgcta
     7381 atgacatgta cgcctaaagg aatcaacaca caccgtttcc tagtgtatgg gaaacgagta
     7441 acctttacaa aatcagaatt aaaagatgaa gaaaatagga aacaaaaatt atcttggaaa
     7501 cggcttttag gaagtactgt ttttctttca gtcatgatca ttggttcgct atttgtttat
     7561 aaaaagaaaa aataaaggag ggtaaccaac atgagaaata gaaaatggct acttgtttgt
     7621 gcagtcgctc ttttttcttt cactgcctac acacagttaa cgtttgcaga cagtcaagaa
     7681 agcacggata gcacagaaca aacaatccaa acgtcagata gcagcacgat tcaaagcagc
     7741 gatgaaaccg tcgattcgtc aacagtggat tcttcagaag aaccgaaaat tgctgaagac
     7801 gatcaaatag tcaaacagac cattcacgtt caaaagatta tttcttcaga acaattagac
     7861 aaccaggggc aaaagcttga aaaacaagaa ggagtaaatg gcgcgaagtt tattgtctac
     7921 gatatcacga atatcttaga agaaatgtca caagatgata atgagaaaaa tcaatctgta
     7981 gaaataattg agtcaaaatt gaaagatcga gcaaaaaaac tttcatacga ccagttgaaa
     8041 aaagtggctg aaggagaaac caaaacgatt gatgatcagg aaggcgtgat tgaattttcc
     8101 attgaagtac ctgcaaacaa aaaacaagca tactatatcg ttaatgaatc aagccctgaa
     8161 aacatttcaa acagtgaaga tattgtttta ttgacacctg tttcaaatga aaaaggtgaa
     8221 tttttgacgg atgtttggtt ataccctaaa tcagagaaat cagagccgaa agaagaaatt
     8281 aaaaaagttg tgtcaactgg tgtaaagaag aacttttttg aaaattgttg ggaatttttt
     8341 gctcaattat tttttgataa ttaggatgaa atgatgaaaa taataagaat attagttttt
     8401 aatctatcct ttttagccgc aacaatattg tgttgcggtt taattttttc tcaattatca
     8461 atttatccaa gatatcaacg attgttcatt cattttctaa acattcaatg tgcttccatg
     8521 ccattagagg tgattaaaaa gctcttgatc aatccaattt ttatggttag tttggttctt
     8581 cttacaggat tggtaacgtg ggtattagga aaaaaagaat gaaaagctta cgatcgttta
     8641 aaaaaatatt agatggaggg cattatggaa aataaaattc attgcactac gggaagagta
     8701 attactttag tgttgttttt gttccttctt ctttttgcga ggggaactgc agtccaggcc
     8761 ttagaaaaag ttgatccgag tcaaatcaaa gaaaataata atctggtgct ttatcatgat
     8821 agtgattcat tgagaaatat cgaagtaact gggatcaatg gatttaagat actagatgag
     8881 tattcaacta aaaaacaaaa agtacttgct tggccaaaag tcaaagattt gaaaagtacg
     8941 tcaaaaatcg ttgcccatta tcaaaaagta ggtacttatc taggaaaaga aattgattgt
     9001 gacgtgactt tttctaattt tgtagcgcac gctggggtta accaagacgg attagctaaa
     9061 acaattaatg gcaaaaagta ttataattat ttgctctttg ataaaaatat ttatcgagga
     9121 tttttcatgc agtcaatcaa tgtcatgaat gcaaaactag aatttaagta tgcaggaaca
     9181 aaaacaaaag tagctttgag tgaaccacag ccagaaattc aatcaaaaat gagatcgatg
     9241 agagcagctg gtattgattt aaacgataca tttctatcca tttacagttt aaatggacag
     9301 tatcagcaac gtgtgtcttg gtataatgac aataatgaat ctgtcggtga acgtaatatt
     9361 gatatgagag aatttgttgg gtatgaaaaa atgggtagct taccttattt tgtcacaaca
     9421 gatacagcat gtgcagaata caaagctcct gcgttatcaa caaacaattt aacttcaaaa
     9481 gtagtgggag gacgtgcaga aaaggcttat agctcgaatg atcatttcac cgatgttgta
     9541 ggagctgata cttatcacag aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca
     9601 ttcatgattg gcgcaaatac gaatagtatg atgtttagct ttgatactgc attgctatgg
     9661 acaccacaac catcgaagcc tacaaaagaa gtgtttaaca aagctaatac tgaagaggca
     9721 gcacacaata ttgacaaaaa agtgattcca caaggatcag atgtttacta tcatattcac
     9781 caaaagtttg atgcattaac agtcaacaca atgaacaaat acaaatcatt taaaatcact
     9841 gatacctttg acagcaaaaa ttttgatatg gtatcggatg ggaaaaacta tgatggcgca
     9901 ttgtggtatg ttgaagcgaa tggtacttgg aaaaaagctg atacaggagg aaagttaact
     9961 gtatcaggta atacagtcac ttatactgct tctgacagct ttttagctaa cgctgcaaat
    10021 tacggtaggg attatatttt gcagctccat atgagagcaa aaaatgatgc aaaaggaatt
    10081 gcaccaaatg tggctactgt aacgtttaac aatgatccga acaaaggaac acaaaaaaca
    10141 aatacagtag aaaactattt ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa
    10201 gatgttgatg gtcgaaatga tggtaaaaaa ggaacgccta cagctccttt aaatgcagga
    10261 tctgaagtac aatatctagt cacacaaaaa tggcatacaa aaggtgtaga cattgcctca
    10321 gatcattaca agcaatttag tattcaagat ccagtggaag atcgtttgac gtataaagaa
    10381 ggttctgcgc gagtgataga tatgagtaca ggaaaagata ttacttcaga aggtacacta
    10441 acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgattt cttaagtaaa
    10501 aatcttttag atggacgaga aattcaactg atatttacag ctaaaactcc tttgaatgaa
    10561 gaattggata tcgacaacca agcaaaagtt tcagtagaaa gtattgagaa agcaactaat
    10621 gtagtaacga ttggggtaaa acctaactta cctcagatca ttgtgccaaa aactggatcg
    10681 aatcatttag tgctaactgt tattggaagt atattatttc ttactttagc atcattgggc
    10741 tatgtagttt taaaactcaa ataaatataa aaacaaggct gagaatcagc cttgttttct
    10801 tttatttgaa gattggattc gcactaataa atacattatc tacgccaatt ctaccagcag
    10861 agtcaactac aaaattatga cctaatccga gaatactttg gttattataa agtaaatttg
    10921 cattatgggc agggctattt ttccatccta ccatggcatc gatagcacca tcgtctgata
    10981 aaaggtcatc aatatacagc ccagtttgac ttatgttttc tcccttagca ccgtagcagc
    11041 ctaagctttc caataaatct tggaaagtac caccaccagg gcgatagtga ctgaagctga
    11101 cttgtaattc tttagctcga atatctgcag cctgttgaac aatatcattg aattgcattg
    11161 gggcaacacc attttgttgg cgataatcat ttaaccaagc aagttctttt tgacgcataa
    11221 agttacgtaa ttcgtctaat gaaatctttt tatatttgaa tgtgattgtt ttattttgat
    11281 cgacgttatt aatggatcgt tggccgtttt tatccacggt aacggtagga tcgtcatttc
    11341 tgcacaagaa ataattttga tcagtcatgt ttttccaagg agtagcaaag ctatcccctt
    11401 cgtatactgt ttttgattca ctagagagaa cagcgttggt atcagcattt acatgattaa
    11461 cagtcacatt aaatttacga gtgtagttga aaatataatt ttgactttca gtcacattgt
    11521 ttaaggttac tgaatttgtt ggttctaccc gatcgtctaa aacatatcct tgaatagaag
    11581 gagcagttaa agtgacactg tcaccgtgag tgacattgac tatctgttct ttcaaaatat
    11641 taactcgtcc ttcgtttttg tagttgttat tgcacaacaa attgatttga tgcacttgat
    11701 tataattgaa tgtgattgtt gtattacctg tgattgtttt gctttgagta tcattaccaa
    11761 tcactttgta gctaaaatta tctttagcgc cttcgctata tttaaatgct tttgccttag
    11821 cagtgtaagt agaaccagaa gttacttttg cttgatctgt agcaatcact tgacctgttt
    11881 gattgtcaat atggttaata gtaaccgtat attcttttgg tgtttcaggt ttgtgttgca
    11941 accctttaaa tgcagcatca agactatttt tagcattatt tacttgggct tgagaagcct
    12001 tagaatcgtc tagtactttt ttagcattgg ataaagcagt ctgaaaatta ttccaagatt
    12061 cttctgtaaa atctccttta gcagttgatt ttactcggtt gtataaagct tgtaattcag
    12121 ctttattcac agtaggaact ggttttttct ttaatccttt gtaagcagaa tctaatttat
    12181 ttttggcact atccacttgt ttttgagtcg cttttgagtc atttaacact ttcttagcat
    12241 tgtttaaagc ggtttggaat gcgttccagg tattgtcggt atagtcacct tttttagttc
    12301 ccttaacgct cgtataaaga gcttctaaag ctgatttatt cactttgggt ttagaggtgt
    12361 cagcgccata ccaaccgata ccttcgtatt tccagccata gtctttggtt aaccattgta
    12421 cttctttcca acttgcagtg tagtgatgtg agccagattt tacaccagga aggaaaaggc
    12481 ggtagatcgg tttattattt ttttcggcag aacgccaggc aggcccttca taagtccagc
    12541 cgtaattttt agtcaaccat ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa
    12601 gacctttatt gtacaaacga tagacaagat ggccaccgct agtcggtgct tcccaggcgg
    12661 ggccttcata atttccccat tttatcttta ctagatggtc acgttctttt ttgctagagg
    12721 tgtaaaaatg ttcatgatta tttggattgt aaaggcgata catgacgtta gttgaagcag
    12781 cgtcaacaga tccagcaaga ctaagtgagg cagcaaatgt tccaatagct aaaacagttt
    12841 ttggattaaa taatttcatt ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt
    12901 attatatatc ggctaaccga tatagtcaat aaaaaataag aaaagcctga tataattttg
    12961 gcttttctta ttttatttgt tagttgaagt attaatatca acgatttctt cagccaattt
    13021 atttaatttc atagcattta tgaatctcat gttgtctata ctagagcgtt tgttggcaag
    13081 gtcactgact gtagttaagg gtacgccaga ttctatggat atagaacgaa ctgatttacc
    13141 actatcaaac agcaaccatt cgattttctt tttatcagcg tacatttcaa tgctccttct
    13201 tgttttttct ttattatagc tcttttttgt aataaatcat aatatcaaga gaaaaatttt
    13261 ctatagaaag gtggattttt atgagtattc gttctccgtg tatccaaatg aggtttgttg
    13321 ctactttcgt tatttgatat actaaaaata aacaacgaaa gaaggaataa aatgaagaaa
    13381 aagcaactat ggtttgtctc tatgtttgca atatttttta ttattttaag tggatgttca
    13441 agtaataaga aatcggaagc taaagaagat aatagtttat tgcaaccagg aactgtttgg
    13501 aaagaagaag taggtggtct tattcataat ttaaaaatta tagatgatac tacctgggaa
    13561 tattctgaaa gtgtatggca tccagatcct gtacaaataa cagttaagag acaaaaagac
    13621 tatgaagggt tagaaagata caaaatagta gattctgcag gtatcagaaa atttataaac
    13681 aaacccgatt ctttatttat cgttgttcct tacgagaaag atggcgtaaa aaaaattgtt
    13741 tttcttggat ctagtaagga tgaaaagcga acgaaagaaa aattgattca tgatggatca
    13801 caatcaaata aatacaaatt acaaaagact agcgagtgat tgctagtctt tttatttacc
    13861 ctaaaggagg aatcatagtg gcaaaagcga aaacgccatt tgaaaaagcc gcgtctaaaa
    13921 tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt gaaaaagcac
    13981 aatttgacct attacgggga aaagacaagg acttagaagg atatatctat caacgtgaac
    14041 gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca attttatgtt
    14101 tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg ttgatatttt
    14161 tagagcatta caaactgccg caactagttt gaaaaataat ctgatgattt ttgctgctgt
    14221 tgcggcagtg gcttccattg ctcttggagg tattttcttc ttctttgggc gaagaggagc
    14281 tgaaaacggg aagacacacg taggttatgt attgttgggg gtaggagtta ttttagctgc
    14341 cgcttctatt gtagtttcaa tgttctcaat gtttggacaa accccacaag gactataaaa
    14401 aaaacaagta gaaggagtaa aaagtacgtg gctacttttt actccttttt ttatgaaagg
    14461 agaatattca tgttttttac gatttcagga aatgcaaaat tggataaaga gttaataaga
    14521 aaagcaacga agaaagtggg aaagcgtact tcctttttac cgaaaacaat taaatattcg
    14581 gtcattgaaa cggaagaaca gctacatgtg ttaggacaag tgttagaaca agagaatcaa
    14641 tcttctgtta aattgactct cacgttacat gataaagaag gaaaaataga aaaggctggc
    14701 acagaatacg tttttggccc actggattat caaatttcag aaggttttta tcctacattg
    14761 atggatttaa tccgccttga tgtgtttaaa gatacggatg aaactatgag tgtggaagag
    14821 tcttatcgaa acgaaaaact tttacaagtt ttagaagaag agttagagag cgaccaaaga
    14881 aaagtgaatc aagtgagtga agtcgaagta aatgaagaag taccatggga acgaccaata
    14941 tttccaaaga aggagtcaaa agaaaaagag acagtctatg ttgacgaaca agctgatatt
    15001 ccgactcaga aatcgatatt tgaggaagag aacgatgtga tgggtcaatt ggaggtagat
    15061 ccctttgaag aaacaatgga ggatgaagag acggacgaag tagaagttga tccagttgaa
    15121 gctaatgaag agtcaacatg gactgaaggg gattctcagg aatatattga accacttatc
    15181 agtgaagaag tagaaaaaaa cgaaacagag atacaaaagg caactgtatt tgattatatt
    15241 ttggatcaat atccgccaga gcatgaatgg atgaaagaaa aaaacaaatt atttttaaat
    15301 gagctttatt cgaaatatca attaccccaa acctatcaag catttttaga gagtaaagaa
    15361 gaattaatct cacagggtaa aggcacactt ttagaacagt tagagtctgt ccaatcaaca
    15421 aattggcatg agcttgcaca agaagattta ttagccacct ttgaagaacg tcaacaagaa
    15481 acagatcaag aaattaatga gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa
    15541 gaggaactaa ataaagaaga acagcgagtg attgaagaag aagtacaaaa aatcaaagag
    15601 cggtacgaaa agaaacgcga caatgcttat tccttgtgtg tagaaaagat caacaatttt
    15661 actaagacga atcaagaagc gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt
    15721 aaagaacgaa aagaagagtt ctaccgcggt ttacgtacag ataagctaaa gattagcaaa
    15781 gaattaaatg aacaactgaa taagctatat caaacaacga ctgaagttct tatggaaaaa
    15841 cacgaacaga tccagcaaga attacaagta caaattccaa aatggaaaaa agagtatgaa
    15901 attcaacaac aagaattgaa agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt
    15961 gaacaattag aaataagaaa acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa
    16021 aaacaacggt tagagcaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa
    16081 caaatggata cattgcgtca agcacagttg aattatctag cacaacctca accagtagct
    16141 gctgttcaaa cgccagtgaa caaccaagca aatttaactc cttcacccca gcgttccttt
    16201 aacggatgga tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta
    16261 tttgctttta atcatatcaa tcaatcttca caggctgatt ctgtagccac aacggcgtca
    16321 atcaaggaag aagcccagga acaagcaaga aaagagtatg aacaacaatt agaagaatta
    16381 ttgaatcgta ccaatcagtc aacaacagag tcaagcatgg aaaatagcaa agagagtgat
    16441 tctagtaagg ctaaagaagg atcaagcgat tcaactgtac aatcaaacac ttccgaaagt
    16501 tcagaatcaa atgaggtgaa ataatgcgtg ttagagaaac aacgaagcga aaagacaaac
    16561 gattcaattt agaaaagaat aggtatcttt ctcatttcta tcgctatact ccctcaggag
    16621 aagagctacc agaaaaacat gacaaacgca tttcgaaaaa gattttttat agtggacttg
    16681 tatacctccc tttagagtat ttagtaacaa gagtaccaga agttttcaac cgattggaaa
    16741 ttgttaaggg aagcttacaa ggaataaatc tgtttcaatt acaggaaatg ccacaggtct
    16801 ttcattttga ttcagctggt ttttggaatc catttactcc tacaactagt ttgggtacac
    16861 gtttagcaat ttcgacagtt tgtgtggttc cagctgtact tatgggattg aaaatggaca
    16921 tgtggtttcg tcctctagca gatggacaaa aaggggataa tcgattaatg accttaaaag
    16981 aaatccaaca gatttatccg caaatacctg aaacaaaaac tacttttcca ggttatggag
    17041 gtattccaat cacacattac aaaaaatatt ggtacattca aacagatact gttaatacgt
    17101 gtattgttgg tacctctcga tcagggaaag gtcagattga agtgttagcc accattgaca
    17161 atttatctcg tgcagaaaaa caaagttcaa tggtcgttaa tgatccaaaa acagaattat
    17221 atgttggtag taaagatgaa ctagaagcta gagggtatga tgtatatgcc tttaatatca
    17281 ccgatccact gcaatcaatg agtgataatc cattggcact catcgtgaaa tactggaaac
    17341 gaggagacat agatactgca acacagttaa ccaatacttt cacaaatacg atttattatg
    17401 atgccaacgc ttcagataac aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc
    17461 ttatttttgc gttgttagaa caagctgatc aaacaggaga ttactccaaa ttgacaccga
    17521 ataatctcgt tgagcttctt tctgagattg gcggttttaa ttattatcca gatccagaca
    17581 atgaattcaa acagttgaat gccttagatg aatttatgaa tcagttgcca cctggaaaca
    17641 ttgcaaaaaa acaatatggg gcaacgaaaa ttggatctga taaagcgaag ggaaatattt
    17701 tatcaacggc aatcacggga ttgaatccat ttactctaac gaaaattgca aagatgactt
    17761 ctcaatcaac gattgattat aaatcggttg ggtttccaaa atacttagat ttgaaattac
    17821 atgaaagtct tttaaatcaa cgagtcgtag tagaatttta tcataaggga aagaaatttc
    17881 atgaagaacg tgtcaaagta ggctataaag gattttgtga aatcaatttt gattgtcatt
    17941 tagttgcagg tgatcaagtt tgtttaaaat acgatcatca aggaaaagaa tatgtggctt
    18001 ggtatgaatt ttctcaaaga gtattgaaag acgaagaggg taatatcgtt tatcggaaaa
    18061 aacgaggaga aacccatttg ccagaattag aaaaagaagc cgttctaaca ttagatgagg
    18121 agaaaagtaa cttattagta gagtctattc ggatgcacta taccgataaa ccaatggcta
    18181 tttttatgct tacacctgat tatgatccat ctaaccatat cattgttagt atttttctta
    18241 gtcagttata taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc
    18301 gcaggattca ttttatttta gatgaatttg ggaatatgcc accaatggat aatatggaag
    18361 gaatcatgac ggtcacagct ggccgaaaca tgttgtggga tctgtttatt caatcttatc
    18421 agcaacttta tgcgaagtat ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga
    18481 cacatattta tattatgtcg acagatgata ataccatcga agaattttct aaaaaagtgg
    18541 ggaataagac ggttgaacaa gagaacagta ccatcaattc attaggtatg aatactcata
    18601 tcaatcgcaa tgttgattca gatcgtatct taactcctga aagaatttct acttttttag
    18661 aaggtgaaac gattgtactc gctcctttaa aacgacaaga tttgaaaggt cataaaatac
    18721 ggccgtatcc tatttttaat catggggaaa cgaatcttcc ttatgcttat caatatattg
    18781 aagatttcaa tccatcgtgg gatatcaatg atatagacat acattgtaca catgcggact
    18841 tggatcttaa ggctttacaa attgattatg attatttcct accagatgaa tatgctagaa
    18901 atctctatca tggactgcat gatcaattag aagaaaaaaa cgaagaaaat atgttttata
    18961 aaatcgttca atctcttgag aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag
    19021 aacaagcagc agatcgactt caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt
    19081 ctgaaatgat tgggaaacgg ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat
    19141 tggaggaatt ttttctggca tagccgactg gtggtcaggc ttattcggag aaggggatgc
    19201 aaaccccaaa accgtaacga aagtcttaca agattatggt aattatctgc agtatcaaga
    19261 tgtatttagt tggatttgga acacttttct gtggggattg attaaaggat tatataatct
    19321 caatacttta ctagaaaaaa ccatttatca aagctttgat ttaaaagact tattgaacgc
    19381 cgctggggta aatgcactat ttcaaagttt gattagtaag gtattggctc ttttttgtgt
    19441 cgtcacatta atttatttag gcttgaaatt ttttatgagt aaacacccac caaaagtcaa
    19501 aaatatcttg gttaatttat ttatggccat gatcttgatt tttggtggta gttctttaat
    19561 cgatgaaggg ttaaatatca gtaaaagctt ttacggagac gtcactgcag caaataaagc
    19621 agataaaacg ggatcacctg cttttcaaat tattcaaaat aatgtgatcg atgtcagtac
    19681 gttactagca actgacccga ataaagtaga atccctacca acagataaaa gaaatgcttt
    19741 aacagcgaaa aattttaaaa cagcgaatat caatgggatt attactcctg atcaagccgc
    19801 tgagatggcg gataacgata aaagtggatt gtcagattta caaaaggatc ggttaaaggc
    19861 attacaatac cgattggaac tagatgataa tgggaaagaa attcctgttc aaattagtga
    19921 cgaaggctta gcaaaatatg tctatacttc agggtatcgt cgatacagta gtaaaccagg
    19981 tattatctta gctggtgaat cttctttggc ggtggcctat ttatttatcc tatttacgat
    20041 cgttacttgc attattgagc tggtatttaa aaaattctat ttagtgattg ccgcgtcaac
    20101 ggactttgag acaggccaac ggatgaaaag tgcgatagaa gatatttctc aatccttctt
    20161 gttattggcg tttacgattt tagaattacg aatctataca ttaatgcttt caggtatcgg
    20221 tgatttgcat gcggcaggaa agatcaatgg attcttgtat gttgttgctt taattgtgct
    20281 aactattgcg ctattcaaag gctctcaatc ggttacgaag attttcggtg ttgatacgag
    20341 tctgaaaaac ggcagtaatt ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa
    20401 tgtgggtagc ggagctaaaa accttgcaag tgctggtaag agtggtttaa gccatttaaa
    20461 tgatattcgt aaaaatggat ttggccatcg aaaagatgtt acccctgata acggggaaga
    20521 acaatcagac gataaagcta caactgtacc taatagtaag aaaacagttt ccgaaatgtt
    20581 cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg tcaatgaacg
    20641 tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct tagaagagaa
    20701 attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat ccgaagcact
    20761 agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg cgtatggtga
    20821 aggacaagtt aatgcagcag taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg
    20881 tatagtgatt ccttcagtca aagatcacca agaagcgttg cactctaatg aaaattcgat
    20941 tgaagagaaa aattccatag aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc
    21001 aaaaactgat ccagaacaat atcgctataa acctaacagt gaccttgaaa atgagaagcg
    21061 aatttcagat ttatcaaaag taaatcaaga agacaaagca gaaaatgagg gacagttacc
    21121 aaaaactgat ccagaacaat atcggtatag atctgttaac tccgaaaatg aaaaacgcat
    21181 ttcagattta tcaaaacgaa atcaagaagt acaaacggat gaggattcca tcgttctgcc
    21241 aaatgataat ggaatcgaag ttggaactga aaaatcaacg aaaattggtt tacctaaggc
    21301 acaaaattta ccaatgaaag atagtcgttt gaatcaattc aaggataatg atcaaagttt
    21361 tgttcatcaa tcacctgcag gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg
    21421 aaatctgaca actgtttctt acaatttatc tgaaagtggt gagacaggga atgcgtccgt
    21481 tcaaaatgtt gtgacaagag aagctgcacc aaaacctatt caaaacgttc atacaggtta
    21541 caagccagca aatgtggcat ttactgcacc aacaaaaata aatgtaacaa gagaaactaa
    21601 aaacaggaac aatgaattga aagaacaatt attgaaggat atcaaatcat aaaaggccta
    21661 cgagttaggc cttttttcta tgtatgaaaa ggagaaatca tttatggata aatatattaa
    21721 catgaaaaat ccaccaagat ttagtgggga atggaagttt ttcgcctttt tctttttaga
    21781 tctattaacg attggtggtt gctttttttg ggcgaaaata gtcaataatc ttgtccactt
    21841 accactcttt tttgcactct tgaattatgg cttatggatt gcgatagctt tagtgattgt
    21901 ttggcgaccc tataataacc caggtcaacg ccagattttt gcgattatcg agtctatttt
    21961 ttttacggac aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta
    22021 taaggaggat gaataaatgc gagtcaaagg tcaaaattca gttgacagaa aatccatttt
    22081 gcagtttgga aaaccagaag agcgctctat cttggactta tttccttata aatcctgtac
    22141 agaagaggga gtcttaatta ctaaagaaga tcattttcaa cgttattatc gtgtagtttc
    22201 tacagatgtg gaaggattaa atgaacaaga gaaactggaa cgaatgaatc agttgaccac
    22261 tgtcatgcgt acgtatgtgc caacgattaa gttagttagt ttgaccacag aaacggatct
    22321 aacggagcaa atcgtgcaaa agcggcagtt attagataaa aatcgtatgg aacagtcctt
    22381 aggaagaaat ctacagcgat taagaaaata tgaaaagaaa ttagtagagg aaatccagga
    22441 attgaaacga gcagaacagg aacgccctga tttaagcttc tttttcgtca ttgaaggaaa
    22501 gacaatcaag gatttgcaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg
    22561 gttaaaacca ctctacaaaa aagaattgat caagattctt tatcgaatga ataatatgaa
    22621 tgatgagtag gaatggggaa gagagaatga aatatccgtt gatatggtta gaaaaagatg
    22681 ttccgaaaga agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa
    22741 aaggaaaatc ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga
    22801 agcaattaaa acagcttttt caccacaaaa tgatcaaaag gtacttgatc cattaaagca
    22861 actaggaaat cgggaaatag aaattttaaa atccaaaggg tatgatttag actttttaaa
    22921 acaagtgatg ccacaaggtg gattaaattt cgatgaagag tatgtgacct atggaaatgg
    22981 ttattcacga tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc
    23041 caatctcatg aacaatcctc atacagtggc aacactagat gttagcacag atgaaaagca
    23101 aaaagtgatt gatcaaatca atcgtgcctt agatgaatta aatgatcggt cagataatga
    23161 acgaaaagcg actgaccgaa ataaaagcgc ggaagaatac ctagaactag cggaatatgc
    23221 ccgaaaattg acttctaatg gggaaatttc taaacaagtc aaagtgagag tacatgttta
    23281 cggaaacacg attcaagaag tagatgaacg agcacaggaa ctgatcgaaa atctcaaagg
    23341 gatggattat cgtgcagtga cctatctgca caaaacgaaa agagactggc aatcgatgtt
    23401 caccatgttc gatgaacaag ctgaatggtt tggaaatatc aaagggcaat cactaccatc
    23461 gctaaacatt ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggtggaac
    23521 acaatatggg gaaaccatga caggcggccc gtttgtttgg aatgccacgt ataaaaatag
    23581 tgtgcggttg tctaccaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt
    23641 tcttaaaatg atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa
    23701 gggaaaagac tttattccct ttttaaagga atataacggc attatagtgc gtttagacgg
    23761 atctgacggg atgatcaatc ctttagaaat atttgccaca cgtacgtatg atgaagcttc
    23821 cagtttatat gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca
    23881 aacgcactta gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac
    23941 gatgaaagca gaatttaaga cctatttaaa tcgtttttat gaagagtatg gaacagtacc
    24001 acgcggtttc acttcctcaa actcgcgttc aaatacggag acgcaagtca cagggaaaga
    24061 tccagaagct tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc
    24121 aggtgcttcc caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt
    24181 atgtgagacg tatggcatga tttttgatgg tcatagtacg attcgacacc ttgaccaaca
    24241 acagttggtc ttttttgaca ttgactctat tacaggttta gatcgtgggg tacaacaatg
    24301 tcaaatgtac atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca
    24361 acgacgttta attaaagaaa aacaaatgga agaacaagac ttcaaatgct tcttggcgat
    24421 gattgatgag tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac
    24481 aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc
    24541 acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt
    24601 tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga
    24661 aggattagtt ggtaaatcaa tgacaaactc agaattagaa tccattcctc aacaaaatca
    24721 aggagacgct gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa
    24781 caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta
    24841 atcaagcagt atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt gtttggtact
    24901 tctgcaggtt tgattactat tttgattact gtgttcgtga ccctttttct aatacttgcg
    24961 gcgggttcga ttgataattc tgattcagat tcaagtagtg gtggcgaagc ttttacagga
    25021 gaatattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat
    25081 gagattgcgc aatatgctgt aggtgcagca gtgaaatata aattattacc aagtgtgatc
    25141 ctttcgcaat acggttacga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc
    25201 aactattttg ggattacgtg gtttgatggg tgtctgtttc ctaaaggaac tgcgcgagga
    25261 atcggtggta ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgcattttcc
    25321 tattacggat ttatggtagc cacacaatcc aattttaatg cttgtgtcgg aaataaaagc
    25381 cctggcgcaa gtttattgat cttaggacga ggcggttatg ccgctgcagg aattactgaa
    25441 gatagtccgt attatacagg ttgtatgtca attatcactt ctaataaatt aaccgaatat
    25501 gatgaattcg ccatcaaaca ttggggtgaa ggtggcaata acaatggcac aattactggt
    25561 gaatggacga atccattccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa
    25621 ttatttggaa caaatccagg cggtgaattc cgtccgaatg gcttccatga tggattagac
    25681 tttggttcag ttgatcatcc aggaagtgaa attcacgcgg tgcatggagg aaaagtcgtg
    25741 tacgttggca atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat taattacgac
    25801 ggtttgaata tggtatatca agaatttgcg aatagtacag gaaattctcg tgtgaaggtt
    25861 ggcgatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta
    25921 ggtttcactc gaatggattg gcgtcaagca caaggacacg cattcacaga tgatggtaca
    25981 tggattgatc cattaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg
    26041 gaaaataaaa ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat
    26101 atcgcgttgt tttggcaact gaaagaagaa caagagacaa atacctttaa aattgaagaa
    26161 gtggctaaaa acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc
    26221 gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgaacagct gaagcaacaa
    26281 gatggcacaa aagcaaaaga agaagtaact gaagcacaaa gtaagacggc agaagagttc
    26341 ggtaaattag ccattgataa atcattaagc agaaatgaat taacggaaaa actaaaagat
    26401 gtcgcaaccc aagaagtgat tgacaaatta agtcctgcag acgatgatca ccaacatgat
    26461 gattacggaa gttactcctt tacattaggt gacgtgcaaa cttacgctca aacaagcagt
    26521 gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattc
    26581 aaagatgtta aaccacaaaa aataaaaggt ggattaactg tcactgaaaa acaggtagat
    26641 ggtcaatgga aagtcactga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa
    26701 gaaaaaagga gaaagacaac gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat
    26761 ctttatgatt ctcggatttt tgattcttgc tagtccgatt tacacgcatc agaaggtaga
    26821 cataccagat gaagtaagca cgaacgcatt tttaaacacg aaggaactaa cgatggtttc
    26881 aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcaatcgacg atgaagatca
    26941 aagcttatta aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg
    27001 ggattatcaa gcaataaaag ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa
    27061 gctatcgcat gtaccagaag aatatattgc tatgcgatta accattattc ctgaaaaaat
    27121 tgatccaaag gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa
    27181 ggttaaagat caagtgaaag atgaagatta tgaacaacat gcgatcaatt acaaagtgaa
    27241 aggatacaag aaagaacaaa aagcagctca aaaacaaatg gaatctttcc aagcaaccat
    27301 tgatttgaat gaagaattag tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga
    27361 tgatcaggaa aatactgaaa acaagattaa tagctaccag caagaaattg aaacatcgaa
    27421 aacgcaaatg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat
    27481 gacaaaagaa aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc
    27541 tgacaaagat actttcgctg agcagaaagt attgattaaa gcctatctca aagttgtaaa
    27601 agctaaagaa atatgtcgag aagtgaattt atctaataca acaatatctg agttgaagaa
    27661 tggtcgaaag aatttagata ctttaaaatt cagatcattt gaagctctat atgtagctgc
    27721 taaaaaatat tttacagctg ataacaaata aaaaacagga gtattttatg tgaaaaaagt
    27781 atataaaaaa tgcttatgaa tcagctatat caccttaacc atttgttaat gagatgggag
    27841 aaaacattaa ccacgattct aacgcaaaca tcaagcacat aaaacattta acactcctcc
    27901 tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag catacacttt
    27961 ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa gttagaagga
    28021 ttttttattg tctaaataca caaataacct taatttataa agagcataag gaaacgtgat
    28081 atcactttct cacgaaaggt gttaggtagg ctgtgcctac cacgtttcta agaaagataa
    28141 atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg
    28201 cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta
    28261 cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat tagaaatatt
    28321 gaagaaaagt atattaataa aattgacaaa cgttgtgaag aacttacggt caagactggc
    28381 aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt tgatcacgct
    28441 cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt ggatattcaa
    28501 acttataata cgaaaacttt agaagaatat attgcaacaa ataatcaatt aattggtttg
    28561 ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca attttggcaa
    28621 aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt gatgaaataa
    28681 tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg tgtatgatgt
    28741 atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat gcaatgggaa
    28801 aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatcttcat ttgaaagata
    28861 caggaatact aattggtaca aattcaactg tttatcaaga agcagaagaa cgtgtggaag
    28921 gaaaaattaa atccaatcaa accaaaaaat attcttctaa gttaaagaaa ccagtgaatg
    28981 aatctgttca taccagagta gatccatttg atccaacgaa gattctaaaa agataaaagt
    29041 agaaggagaa cgtgatgaat aaatcgcctg cagttattct tacttgtcaa tttaccttgc
    29101 ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa gcactggaag
    29161 aaatggaacg acgtacgccc gaagaagaac aggaactgca acgaatcaag aatgctttag
    29221 aggattttta tattcctgaa ggagaaactt tttcggaaag taagacggat aaagaattca
    29281 gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt gacaagttag
    29341 aggaacaaga ttttaccaaa tatctttctt atatgacccg tcactatgcg ttagaacaaa
    29401 agaaagaact gacgcaaagg gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa
    29461 aatatcaaac agagccacat aaaaaagctt caggaaatct tcctggagtt ttttctatgg
    29521 cttcagacga agtgaaagga agtgatctga aggagatcaa acaaatcttc caaagaggac
    29581 aacaaaaagg ctcaatcatt tatcaagatg ttatctctca cgacaattcg tatcttgaaa
    29641 aaatgggctt gtacgatccc aaaacagata ccttagatga agaaggtttg aaagcagctg
    29701 gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca ggttactgga
    29761 tggcaacgat tcaccgtaac actaagcata tccatattca ttttgcagtt gttgaaaaag
    29821 aaaatacgcg taaaccgtat actgagattg aaaatggtgt gagttatatc gtaccgaagg
    29881 ggaagagaag acaagcaaca ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac
    29941 gtttctctta tgaaaaggat cgaatgatcg gtatggagaa acgaaattta ttgacacgaa
    30001 aaagtgattt gcgtaacacc ttaacaaaag tagtaaaaga accagcacgt tacgatcgac
    30061 acgccatgaa cttattaaat gaagtatata aaaccctacc tgaaaaacgt tcggaatgga
    30121 attatggaga tgaaaatcga accaagcttt caccatttac aaggggaaaa cttgatgagt
    30181 taaccaaata tgttttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
    30241 cattgaaaga agaagccaaa agcctttatg gggaaagcaa aagagaaaca aagaatgcag
    30301 aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgcgatttta acagaactga
    30361 agagacagga tagtcaaata aaaccattaa tagaaagtgt caatcagatg caatctattt
    30421 acaatgaacg atcctttcga acactcaaac agttgccaac aaatgaatac aagtatagtt
    30481 atcaagaatt cgtaaagcgt aggaaacaat tccaacaaag agttgtcacg cagcgaagct
    30541 tgcgtcaatt aaatcgaaca attaatgcac atgaagaaaa ataccacgca agtaaagcat
    30601 atgaagagca acagcgaaga atccaaatgg aacaagaaag acaataggag gaagagaaat
    30661 gaaacaatat ttagatcaat ggaaagtcat agaaggatca ctaaaagaag aacgaatcga
    30721 acaacttcct gattgtttag aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa
    30781 cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg gtgtgtattg
    30841 ttgtaatcat gtgaagggtg aaaaatattt tattattcaa gaatacgaag gtaagttagc
    30901 gccttattat actacttggg aaataaatga agagggaatc aataatttcc catgtgaaag
    30961 cattgaagag tctatttctc tcacggaatg ttaaataaat agagacgtaa aagagtcctg
    31021 aacgtgattg tccaggactc tttttcaatg aattgttttc cgccaattca ctgagtggtg
    31081 cgattcaatt ttattatatc aaaagaaagt gagattccaa agtgaaggga ggaagaagaa
    31141 atgactcaga caacgattgg tgttgtggtt gaaaaaagca gggataatct cgtttttgtg
    31201 acagaaattc aaacaggtag agcttttgta gtcacagaca aagctgcaaa agcgtatcaa
    31261 aatggcaata ttttaacgtt aaatctgact acgaagatgt tcgttgatgc agtagaaaat
    31321 tatccatttg tttagacaaa cgatatttta caaggaggaa acggacatgg cagaagaaaa
    31381 agaaccacta gataatagtc gtttgggaaa gtccaaaaga aaattagtac ggttgcaaaa
    31441 cgaattaaat aatcaatttc aaaagctatt tgatcaccaa aaattgacaa atgggcaacc
    31501 aatgaatgac aaacgaaatg gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa
    31561 aatacaaagt ttgagagaag agatcaagca acaagaaaaa caggtagaaa aacttgaaag
    31621 acaagaagag ctaaaggaaa tgggttataa caagtacggt ggcctggaca tgacgatcga
    31681 aaatattcca agaatcaaag aagaaatcga acgctttgaa aaaggtgaat cgactttttc
    31741 agctgccacg atccgaaaat accaacgaaa attggaaaca ttagaacagc tgaaagaaaa
    31801 atcagagaaa ggaaaagaaa atcttcttcc tgaagtacaa gcgatcattg acagcggacg
    31861 agtgacgcaa tggaaaaaga atccaactat ctattttctg aaaggctatc gaaaagtcgc
    31921 tttagaactc aatgaaaacg ggggatttga ggaatctaaa aaataccaag ccacgtcgga
    31981 agaagaaaag gcagctgttc gtgaattact tgaagaagct acacctactg attctgtgaa
    32041 ggagaaagaa accgatcatt tctatcgagt agaattcaac gaaaatgatc cagcaaaagg
    32101 gttgaaaagt tatggtgggg agatcgtaac actagaattg atcgatcaac tcaaaacact
    32161 ggataaccaa tacgccgcag aagcaggcta ttataaattc tattttgaag aagtgaagaa
    32221 tggagaagta gtcgatcaga tacgaatgga tctaggggat ggcgtcaata gtaatcaccc
    32281 catctattta gagttagcta gattgtgcga acaatcacct gaaaaggaac aagaacaaac
    32341 agtcaaaaat gaacctattt caaaagtaca aaacgagatt aaagagacaa aaacaaaaca
    32401 aaagggttat caaaaacgaa cggcagaaga gattaagcaa gaagtcaaat cactttccca
    32461 acaggcgctt gatgcggtga aaaagcatac tcattcacct gaagacgtca aagaactttt
    32521 gaattttatg tctcgttttc cagaacgatc ctttagaaat caattattga tcgaggaaca
    32581 atttccaggc gcaacggctt gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat
    32641 caaaaaagga gaacaaggga acaagatctt tgtcagaaaa acggtcaaag gattttatga
    32701 gaaaggacgt ggctttgttc gagaaacaga ggctacacca gaagataaaa aacgaatcga
    32761 aagtggtcaa atcgaagtga tcaaaaaacc ttactacacg attgaaaaag tgtttgatat
    32821 cacacaaaca caattgaaac ctgaagacta tccgaaaatc ttcccgaacc gcgtctttga
    32881 ttttcaattg gatcaaacag ggcagacaga acttcaagtt gggattcaag cggtagccaa
    32941 aaatattgga attcaaattc gagatatgac agaaagtgaa gtctatcaaa gagaattagg
    33001 acaagcacgt ggggcgtacg ttcgtaacga atttactggc gaagaagaaa tcgtcatgaa
    33061 tactcgaaat actcctacgc agcatttagc cacaagtatt cacgaattag cccatgctag
    33121 aatgcacaaa ttttcagaat tagatactgc aacaaaagaa ctacaggcag aaatgacctc
    33181 ctatattgtg tgcaagcatt ttgggatgga cacctcggag aaagctattc cttatattgc
    33241 gacatggacg aagaatggtc agacgttgga tgataaggaa gctgcggaac gtgggaaaat
    33301 catgaatgat gtcagtcgtg tcgcaaatga attcattcaa accatttcta atgaaatcaa
    33361 tcaatacaga gaaagagaac cagagatcca gccagcagaa gtgaagcagg agcataaaaa
    33421 tactgtagaa acggcagaac aaaagaatat tgtgcccgtt ggctcacttt ggatcgacaa
    33481 gaaagacggg aaagtcctgg aagaagacac gggccgaacg ttacatttga aggatgcggc
    33541 gtttattgaa gatccgcagc ataatcaagt gattttaaaa ggaaaaagtt atggaaaaga
    33601 gatccaaacg gcgttacctt cagaagaatt tgcgctagga aatccattcc gattttcact
    33661 tgagacagtg tcagaagtgg caaaacgaaa aaatgaaatc tattatgaac aacaattgtc
    33721 acaaaaagaa cgcgtataaa acaagcaaga aagactatct gaaaaatgga tagtcttttt
    33781 tcttttggga ggtttgaaaa tgacagagaa aaaaatgagt ctcattgatc gatgcaagca
    33841 aatggatatt gtggattttg cacgaaacaa tggtttagcg gtgg
//
LOCUS       DADJYG010000012        31455 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.012, whole genome shotgun sequence.
ACCESSION   DADJYG010000012 DADJYG010000000
VERSION     DADJYG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.012"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            212..604
                     /locus_tag="JZ382_000532"
     CDS             212..604
                     /locus_tag="JZ382_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY2426740.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="JZ382_000533"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="JZ382_000533"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAY2426741.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="JZ382_000534"
     CDS             3165..4775
                     /locus_tag="JZ382_000534"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAY2426742.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="JZ382_000535"
     CDS             5067..5936
                     /locus_tag="JZ382_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAY2426743.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="JZ382_000536"
     CDS             6101..7372
                     /locus_tag="JZ382_000536"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY2426744.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="JZ382_000537"
     CDS             7369..8664
                     /locus_tag="JZ382_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY2426745.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="JZ382_000538"
     CDS             8763..9026
                     /locus_tag="JZ382_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAY2426746.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="JZ382_000539"
     CDS             9471..9920
                     /locus_tag="JZ382_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2426747.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="JZ382_000540"
     CDS             9977..11395
                     /locus_tag="JZ382_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2426748.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="JZ382_000541"
     CDS             11395..12255
                     /locus_tag="JZ382_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY2426749.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="JZ382_000542"
     CDS             12429..13202
                     /locus_tag="JZ382_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY2426750.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="JZ382_000543"
     CDS             13349..13714
                     /locus_tag="JZ382_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAY2426751.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="JZ382_000544"
     CDS             complement(13862..15070)
                     /locus_tag="JZ382_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2426752.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="JZ382_000545"
     CDS             complement(15089..15367)
                     /locus_tag="JZ382_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426753.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="JZ382_000546"
     CDS             15560..16216
                     /locus_tag="JZ382_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAY2426754.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="JZ382_000547"
     CDS             complement(16658..17455)
                     /locus_tag="JZ382_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAY2426755.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="JZ382_000548"
     CDS             complement(17452..18636)
                     /locus_tag="JZ382_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAY2426756.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="JZ382_000549"
     CDS             complement(18661..19908)
                     /locus_tag="JZ382_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAY2426757.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="JZ382_000550"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="JZ382_000550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY2426758.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="JZ382_000551"
     CDS             21888..22202
                     /locus_tag="JZ382_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY2426759.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="JZ382_000552"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="JZ382_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY2426760.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="JZ382_000553"
     CDS             22887..23531
                     /locus_tag="JZ382_000553"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY2426761.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="JZ382_000554"
     CDS             23546..23875
                     /locus_tag="JZ382_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426762.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="JZ382_000555"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="JZ382_000555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY2426763.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="JZ382_000556"
     CDS             24863..25687
                     /locus_tag="JZ382_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAY2426764.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="JZ382_000557"
     CDS             25680..26027
                     /locus_tag="JZ382_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAY2426765.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="JZ382_000558"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="JZ382_000558"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY2426766.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="JZ382_000559"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="JZ382_000559"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY2426767.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="JZ382_000560"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="JZ382_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAY2426768.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="JZ382_000561"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="JZ382_000561"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY2426769.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10111 a         5635 c         6634 g         9075 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgt
//
LOCUS       DADJYG010000013        31244 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.013, whole genome shotgun sequence.
ACCESSION   DADJYG010000013 DADJYG010000000
VERSION     DADJYG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.013"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            111..1247
                     /locus_tag="JZ382_000562"
     CDS             111..1247
                     /locus_tag="JZ382_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY2426770.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1384..1728
                     /locus_tag="JZ382_000563"
     CDS             1384..1728
                     /locus_tag="JZ382_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAY2426771.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            1999..3285
                     /gene="efmA"
                     /locus_tag="JZ382_000564"
     CDS             1999..3285
                     /gene="efmA"
                     /locus_tag="JZ382_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAY2426772.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3423..4022)
                     /locus_tag="JZ382_000565"
     CDS             complement(3423..4022)
                     /locus_tag="JZ382_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAY2426773.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4426..5547
                     /gene="mnmA"
                     /locus_tag="JZ382_000566"
     CDS             4426..5547
                     /gene="mnmA"
                     /locus_tag="JZ382_000566"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY2426774.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5793..7214
                     /gene="arcD"
                     /locus_tag="JZ382_000567"
     CDS             5793..7214
                     /gene="arcD"
                     /locus_tag="JZ382_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAY2426775.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7228..8346
                     /locus_tag="JZ382_000568"
     CDS             7228..8346
                     /locus_tag="JZ382_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAY2426776.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8406..9698)
                     /locus_tag="JZ382_000569"
     CDS             complement(8406..9698)
                     /locus_tag="JZ382_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY2426777.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9848..10408
                     /locus_tag="JZ382_000570"
     CDS             9848..10408
                     /locus_tag="JZ382_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2426778.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10537..10914
                     /locus_tag="JZ382_000571"
     CDS             10537..10914
                     /locus_tag="JZ382_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAY2426779.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10847..12919
                     /locus_tag="JZ382_000572"
     CDS             10847..12919
                     /locus_tag="JZ382_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY2426780.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13130..14077)
                     /locus_tag="JZ382_000573"
     CDS             complement(13130..14077)
                     /locus_tag="JZ382_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY2426781.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14323..14787
                     /locus_tag="JZ382_000574"
     CDS             14323..14787
                     /locus_tag="JZ382_000574"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY2426782.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14849..15892)
                     /locus_tag="JZ382_000575"
     CDS             complement(14849..15892)
                     /locus_tag="JZ382_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAY2426783.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16269..17234)
                     /locus_tag="JZ382_000576"
     CDS             complement(16269..17234)
                     /locus_tag="JZ382_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAY2426784.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17468..18313
                     /locus_tag="JZ382_000577"
     CDS             17468..18313
                     /locus_tag="JZ382_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2426785.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18504..19691
                     /locus_tag="JZ382_000578"
     CDS             18504..19691
                     /locus_tag="JZ382_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAY2426786.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19697..20053
                     /locus_tag="JZ382_000579"
     CDS             19697..20053
                     /locus_tag="JZ382_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAY2426787.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20055..20393
                     /locus_tag="JZ382_000580"
     CDS             20055..20393
                     /locus_tag="JZ382_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAY2426788.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20788..21822
                     /locus_tag="JZ382_000581"
     CDS             20788..21822
                     /locus_tag="JZ382_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2426789.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21815..22501
                     /locus_tag="JZ382_000582"
     CDS             21815..22501
                     /locus_tag="JZ382_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426790.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22525..23373
                     /locus_tag="JZ382_000583"
     CDS             22525..23373
                     /locus_tag="JZ382_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2426791.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23570..24343
                     /gene="sufC"
                     /locus_tag="JZ382_000584"
     CDS             23570..24343
                     /gene="sufC"
                     /locus_tag="JZ382_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY2426792.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24356..25642
                     /gene="sufD"
                     /locus_tag="JZ382_000585"
     CDS             24356..25642
                     /gene="sufD"
                     /locus_tag="JZ382_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY2426793.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25639..26874
                     /locus_tag="JZ382_000586"
     CDS             25639..26874
                     /locus_tag="JZ382_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY2426794.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26861..27331
                     /locus_tag="JZ382_000587"
     CDS             26861..27331
                     /locus_tag="JZ382_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAY2426795.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27336..28727
                     /gene="sufB"
                     /locus_tag="JZ382_000588"
     CDS             27336..28727
                     /gene="sufB"
                     /locus_tag="JZ382_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY2426796.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28822..29964)
                     /locus_tag="JZ382_000589"
     CDS             complement(28822..29964)
                     /locus_tag="JZ382_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2426797.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30003..30737)
                     /locus_tag="JZ382_000590"
     CDS             complement(30003..30737)
                     /locus_tag="JZ382_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2426798.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            30969..31244
                     /locus_tag="JZ382_000591"
     CDS             30969..31244
                     /locus_tag="JZ382_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2426799.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
BASE COUNT        10164 a         5718 c         6588 g         8774 t
ORIGIN      
        1 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
       61 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
      121 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
      181 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
      241 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
      301 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
      361 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
      421 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
      481 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
      541 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
      601 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
      661 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
      721 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
      781 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
      841 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
      901 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
      961 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
     1021 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
     1081 cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag
     1141 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
     1201 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
     1261 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
     1321 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
     1381 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
     1441 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
     1501 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
     1561 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
     1621 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
     1681 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
     1741 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
     1801 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
     1861 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
     1921 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
     1981 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
     2041 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
     2101 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
     2161 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
     2221 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
     2281 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
     2341 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
     2401 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
     2461 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
     2521 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
     2581 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
     2641 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
     2701 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
     2761 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
     2821 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
     2881 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
     2941 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
     3001 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
     3061 gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca
     3121 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
     3181 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
     3241 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
     3301 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
     3361 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
     3421 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
     3481 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
     3541 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
     3601 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
     3661 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
     3721 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
     3781 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
     3841 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
     3901 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
     3961 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
     4021 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
     4081 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
     4141 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
     4201 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
     4261 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
     4321 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
     4381 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
     4441 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
     4501 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
     4561 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
     4621 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
     4681 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
     4741 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
     4801 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
     4861 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
     4921 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
     4981 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
     5041 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
     5101 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
     5161 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
     5221 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
     5281 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
     5341 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
     5401 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
     5461 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
     5521 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
     5581 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
     5641 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
     5701 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
     5761 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
     5821 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
     5881 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
     5941 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
     6001 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
     6061 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
     6121 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
     6181 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
     6241 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
     6301 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
     6361 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
     6421 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
     6481 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
     6541 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
     6601 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
     6661 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
     6721 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
     6781 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
     6841 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
     6901 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
     6961 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
     7021 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
     7081 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
     7141 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
     7201 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
     7261 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
     7321 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
     7381 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
     7441 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
     7501 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
     7561 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
     7621 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
     7681 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
     7741 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
     7801 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
     7861 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
     7921 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
     7981 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
     8041 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
     8101 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
     8161 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
     8221 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
     8281 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
     8341 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
     8401 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
     8461 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
     8521 accaaaacaa gaagagaagt cgaaagaaag acgccacgat aaccaaatgc actggaaaca
     8581 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
     8641 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
     8701 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
     8761 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
     8821 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
     8881 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
     8941 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
     9001 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
     9061 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
     9121 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
     9181 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
     9241 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
     9301 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
     9361 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
     9421 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
     9481 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
     9541 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
     9601 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
     9661 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
     9721 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
     9781 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
     9841 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
     9901 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
     9961 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    10021 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    10081 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    10141 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    10201 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    10261 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    10321 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    10381 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    10441 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    10501 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    10561 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    10621 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    10681 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    10741 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    10801 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    10861 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    10921 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    10981 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    11041 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    11101 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    11161 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    11221 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    11281 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    11341 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    11401 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    11461 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    11521 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    11581 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    11641 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    11701 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    11761 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    11821 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    11881 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    11941 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    12001 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    12061 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    12121 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    12181 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    12241 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    12301 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gattgcaagt
    12361 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    12421 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    12481 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    12541 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    12601 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    12661 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    12721 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    12781 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    12841 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    12901 agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa
    12961 gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt
    13021 ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt
    13081 gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag
    13141 gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt
    13201 tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga
    13261 ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa
    13321 acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag
    13381 cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg
    13441 agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt
    13501 cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt
    13561 tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac
    13621 gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg
    13681 caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat
    13741 cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca
    13801 gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag
    13861 ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg
    13921 ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt
    13981 cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt
    14041 cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat
    14101 gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga
    14161 ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc
    14221 ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag
    14281 aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct
    14341 atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca
    14401 atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa
    14461 gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg
    14521 ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg
    14581 tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca
    14641 aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga
    14701 aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg
    14761 ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga
    14821 gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag
    14881 ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt
    14941 tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc
    15001 agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga
    15061 ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct
    15121 gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca
    15181 gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga
    15241 caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac
    15301 cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag
    15361 ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt
    15421 cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag
    15481 cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca
    15541 agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac
    15601 caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta
    15661 atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat
    15721 ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa
    15781 tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt
    15841 cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg
    15901 ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt
    15961 tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg
    16021 gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa
    16081 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt cttttacgac ctcttggtaa
    16141 acggtgttca aaagcttgca tctgcggtaa taagcccaac cagtacaaaa acatgaacag
    16201 ctgtttttgt ttggccgttc ttattacttg atgcagaaca gctttttcac aacctattga
    16261 atcgattatt aagcgatttt tttagaaaca aattttccat gtttgctatg ttctttaatt
    16321 aagaaacgta cgtaatgagc aagaacgtag aagacaacta cacttgcaaa cagacattcc
    16381 gcaactttta agaaatccaa tacgctacta tagataccta aactgttgaa gtaatgaaca
    16441 gtagtaaata tagctgttgc agagataaca agcgggaatg taaaggctgc atagcttgga
    16501 taaaaacgaa gcttgaacaa acgtggaagc atagacagca caacgaaata caatacttga
    16561 gataaagcga acaatgctac gacaagataa aaattaggtt cagcaaagtt tttaagataa
    16621 cctgttaggc aaagagaacc aggtgctgca acgatcgtaa tcagcggacg tgtcggatga
    16681 gccatatttc ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa cgcatagcag
    16741 atcaatgaaa tccaaaagaa aatttttcca ataagtggga agaagttccc ggcagttaca
    16801 gtgatgaccc caaatccaac gtaaatgata aaccagcttg gataaattgc ttcaattgaa
    16861 agatcagctt tgataacatg gtgataattg aaataaaaga caaggatcac ttggaagata
    16921 acagcgcaca gccaaatgaa tttgacaatt ggtgcaatac tttcatatga aacaaaataa
    16981 gtacaaataa ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat gattggatca
    17041 tgcaacgttt gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat gaatatcata
    17101 agaatcatag agataccgcc agttagattg cctaaagcaa catggtgata gtctttaaac
    17161 agatttccaa gggaagctaa cccaaggatc aacccgcaga tcgggatcgg aataattttc
    17221 aaaaagttct tcatatataa tattctcctt tttatttaat agattttagt accaagatga
    17281 acgaatacat tgtaaaatga ataggaatgt atgattacga gcaagcagac agctgattgt
    17341 cttcatctaa gttactagtt ggatcagcaa ctgttttgtt gtacatttct attatagatg
    17401 gttttttgta tatttaaatc aaatagtttt tatagtaatt atcataaaaa gttatggaag
    17461 tggtgccatg tttaagttat taacgacttt tcgagcagta tatgaaacaa gaaatttttc
    17521 cagagcagca gaagtactgt ttttatctca accagcagtc tcgaatcaaa tcaaacagtt
    17581 ggagcgggaa ttgaatatcg atctttttca acgtaacgga agaaaagaga tgcttccaac
    17641 taagcaagcc gacttattat atgaacggac actggttttg ttagaagaat gggaagaaac
    17701 tcagcaaaaa ttgctcaatg aacaaaatga agaagaagta tgtcggatcg ttgcttccca
    17761 cacctttgct gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa
    17821 agtgcatttt gagatcgaaa tcacgaattc tcatgaatca ttagaacaag tagcgaaaca
    17881 tgaatccgat tttggtttta tcgaaaagcc attagagact tccggcgtac aaagatactc
    17941 tttgatggaa gaccagctag tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag
    18001 agaagcaaac tctggtgttt ttcattacac gcagcgttat tttgaagaga tgaatatcca
    18061 agataagaag atgatcgtga aaagtaacga tgtaattgtt gcgttgctga aggaaggaat
    18121 cggtcgctcg attttgtcta agcgctctgt tccagaaaat gtgccaatga ctccgttgaa
    18181 tttccatcga tatttttatt tcatccaacg aaccaatctg gtttctgatc agctgctaga
    18241 gattgccgaa gagatccttc gatacaatga aagcacagaa acttctcaca gcgaggagaa
    18301 tctcagctgg tagataattg catgtatcaa aaaaagttct atgatacact aaagataagc
    18361 agtaggcttt tgtactgcat ttgctcaagc aactgcacaa acggaaagtt aatttttccc
    18421 aaatgtctat ggaaagactt tccattatct aactggaccg ctataataat cagcagtatg
    18481 gtttttaaga aaagggatga aacatggaaa aaaaattaaa agcttcgaac gataatcgta
    18541 ttgattatgg cgtgatttta ccagtctttc tgttatgttt gattggtctg ctctcattat
    18601 atgtagcact tagccatgat ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag
    18661 ttgtctggta tttagttgga gcggcagcaa tcgttgtcat catgcatttg aattccaaat
    18721 ggatctggaa actgactcct tacctttatg gtttggggtt agtggtaatg ggactcttgc
    18781 tcaaattcta tgatcagaat ttagctgctt caacagggtc taaaaactgg tttcgattcg
    18841 gtgcgtttac tttccagcca gcagagttga tgaagattgc gtacatcttg atgatggcac
    18901 tgatcgtaac taaacataat acgcaagtaa aagaacggac gatgaaatcg gacttttggc
    18961 tgattgggaa gctcttgatc gtgacaatac cggttttagc attgatcatg gcccaagatg
    19021 actttggaac gatgcttgtc tttcttgcga tctttggcgg gattttcctg atgtctggaa
    19081 tctcttggcg catcatcgca cctgtagtaa tcttggcagt agtcgttgga gcaggaacga
    19141 tcttcttggt tacgactgaa ggcggaagag acttgcttta taaagttgga ttcaaatctt
    19201 atcagtttgc acgtattgat tcatggctgg accctttcca tgatacgtca ggaatgagtt
    19261 atcagccggc ccaaggactt ttggcaattg gtaccggtgg cttgtttggt aaaggattca
    19321 acgtaagcaa tatctatgtg cctgttcgtg agtccgatat gatttttacc gttatcggtg
    19381 aaaacttcgg ctttatcggt ggtgcattcg tcattttctt gtattttatt ttaatctatc
    19441 gtatgattcg ggtgtgtttc gatacgaata atgaatttta cgcctatatc gcaagcggtt
    19501 tgatcatgat gctgctgttc catgtctttg agaatattgg agcaaatatc ggattacttc
    19561 ctttaacagg gatcccattg cctttcatta gtcagggagg atcctcgatt ttagggaaca
    19621 tgatcggtat cgggttgata ttatcgatga gataccaaaa tgaagcacct gcaagatctt
    19681 caaggaggta agaaaattgt acacctttta ctggtacccg aaatgttcaa catgcaaaaa
    19741 agccaaagcg tggttagacc agcatggcgt tagttatcaa accgttgata tgatcaaaga
    19801 agtaccgaag gcctcaacga ttgagacttg gctagaaacg agtcaagtac caatccgtcg
    19861 cctgttcaac acaagcggga tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga
    19921 gtttacggct tcaaaagcga gccaagtctt atcgacagat ggtatgctga tcaagcgacc
    19981 actgatctta cgagaggacc agcttgttgc aattggattc aatgaaaaaa tttatgaagg
    20041 agaactgata taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat
    20101 ttaatggaaa agaatactgt gttggtttga caaatgaagc gcaagaagaa cttggagaca
    20161 tcacgtttgc caatctgcca aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg
    20221 aagttgaagc ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt acagtttctt
    20281 ctgttaatga taaaatcggt gaagatatca atatcttgaa tgattcagat gaaatgaatg
    20341 cgtggatctt aagtttcaaa gatgttgatc caaaagaatt tgacgaatta taaaatcaga
    20401 aaatcacttg aaaaaagttg acactgattc ttccagttgc tataatcata agcaatccta
    20461 tatgaaacga gaaagctttg aaaagaagag tacatgtcac ggacgtttga agagagccct
    20521 gtttgctgaa aatgggtaac gaaaatgaga tggaagatgg tcttagagca gagtaagcga
    20581 gcgtaagcaa acttactacg ggagtgcccg ttacagcgcg acagtatacg gaaagaagct
    20641 aagtccaagt actggaagag gctgttattg cgaagtaacg gtaaatcaag gtggtaacac
    20701 ggaagttcaa gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa
    20761 aaatcataag agaaagatgt gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag
    20821 acattcaatg ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact aaacgttgaa
    20881 aaaggggata tttatggaat cgtcggctat tctggcgcag gaaaaagtac attagtccgt
    20941 ctcctgaatg gacttgagct tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt
    21001 acaaaattaa aaaacaaaga attacgtaca ttccgaaaga aaatcggtat gatcttccag
    21061 catttcaacc tgttatggtc aagaactgta cttgaaaata tccagctgcc gctggaatta
    21121 gctggcgtac caaagagcaa acgtaaagaa cgggcagaag aactgttgcg tttagtcggg
    21181 ttagaaggac gcggacaagc atatccttca caactttccg gcggacagaa acaacgcgtc
    21241 ggtatcgcac gagcattggc aaatgatcct gagatcctgc tttgtgatga ggctactagc
    21301 gcattggacc cgcaaacaac agaagaagta ttagacttat tgttagcgat caataagaaa
    21361 ttgaatttga ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa aatctgcaac
    21421 aaagtcgctg tgatggagtt ggggaaagtc gtagaagaag gagacgtatt gactgtcttc
    21481 agacaaccga aaaaagaagt aaccaaacgc tttgtacaac aagacatcga accagaagct
    21541 gattcgacag aactattagc tgaactcatc aaagaaaatc caagcggccg tttggcaact
    21601 ttgaccttta gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat tcgacgctat
    21661 aacatcgatg tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg ttcctttggt
    21721 tcattgactg tcttgatgac tggtgaaacc aaagaattag atcaagcaga agtgttcatc
    21781 aaagaacaag gagtaggaat tgaggtgatc catcgtggat aaaagctttt cagaaacata
    21841 tctagatttt aaacaaatca acccggaaac gatgaatcag gctatccttg atacactata
    21901 tatgacatta atctcaatgg tcttagtgac gatcatcgga ttagttttgg gattgatcct
    21961 ttactctatt ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa tcgtatcgat
    22021 catcagtaat attttccggt caacgccatt tatgatcctg atggttttga tcattccatt
    22081 tactaaagca atcgtaggca ctatgctagg tgcgcaagca gccattccag ctcttgtttt
    22141 atccgcagca ccattttatg cccgtctagt agagattgct tttcgtgaag tcagccctgg
    22201 tgtactggaa gcagcagatg caatgggagc aagttactgg gaaatcatct ggaaggtctt
    22261 gatacctgaa agtattcccg cattgatttc tggattaaca gttacaacga tctcgatgat
    22321 cggatttact gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca
    22381 ggaaggctac cagcgtggta atctaacagt gacattcgtt gcgactttaa ttattttact
    22441 aatcgtattt atcgttcaag gaatcggtga tttcttaact aagaaaacag acaaaagata
    22501 ataggatata gggggatgta ggatatgaaa aagaaaattt ttggatttgc agcagcgctt
    22561 ttgctgactg taggattagc agcatgcggt aacaacagcg acagcaaaga ttcagcaaat
    22621 aaagcagacg acacgacatt gaaagtcggc gcttcaccga caccgcacgc agagattttg
    22681 gaacatgtga aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat
    22741 gactatgtat taccgaacca atctttggaa gaaggcgaca ttgatgccaa ttacttccag
    22801 catattcctt atttgaacaa agaaatcaaa gaaaaaggat atgactttgt caatgccggt
    22861 gctgtccaca tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta
    22921 aaagatggtg caacaatcat tacttcaaat tctgaatcag actggggacg catcatcaca
    22981 attctacaag atgctggctt agtcaaagtc aaagatggag tagatctgga aacagcaaca
    23041 ttcgatgata ttgaagaaaa ccctaagaac ttgaagttca accatacgat cgacccatca
    23101 cttttagctt ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc
    23161 gcttacggcg cagggttgaa tccattagaa gatgccgtat tgcttgaaaa ggacaactct
    23221 ccttacgtga atattattgc gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa
    23281 ctgattgaag tgcttcaccg caaagaagtc caagaatgga tcacagacaa atggggcggc
    23341 tctgttaaac cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata
    23401 agtctgtaat ggcttatttc tcatcttttt tatacctatt ctaatttaga aatcaaaatt
    23461 gtttaataat aaataaaaga tatttgattt tagtaaggaa ggtcattgcg cttcattctc
    23521 aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca tgtctgtctt
    23581 agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct
    23641 aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag gaaaatctac
    23701 cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg aaattttgtt
    23761 tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg gcttattttt
    23821 agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca tgcgtgcagc
    23881 gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt
    23941 agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt atctgaacga
    24001 aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga tgttagaacc
    24061 tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt tgaaagtcgt
    24121 tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca tcacccacta
    24181 tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg aaggccgagt
    24241 agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct acgctggtat
    24301 cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag caagaatgaa
    24361 agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga
    24421 acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg aactgccatt
    24481 gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga aagaaacaca
    24541 aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca atcctgtgct
    24601 tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag cagaaaaagg
    24661 agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag tccaagaata
    24721 ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc atgtagcttt
    24781 tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga
    24841 gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt
    24901 ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca aagaacaagt
    24961 agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa
    25021 atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc gccgtgggca
    25081 cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg gtcatgtgat
    25141 cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca aaatcgtggc
    25201 aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata aagcccctca
    25261 tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat tgacctttaa
    25321 tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt
    25381 cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga tcgatgaaaa
    25441 cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag aaatgtacta
    25501 tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc gaggattcct
    25561 cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag tcgaagtaat
    25621 cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt tcctatctta
    25681 tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac gacccaaaag
    25741 ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc caatgttcat
    25801 cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc aagagaaaaa
    25861 gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg
    25921 acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc aggagacgag
    25981 atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca gttggcaaaa
    26041 cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct ggatatggag
    26101 gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca tgtatccaat
    26161 gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga aaacggcggg
    26221 gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta
    26281 gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg tattggtgtt
    26341 ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg tggcgaaatg
    26401 atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa attcgaagca
    26461 gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta tttgcaaaaa
    26521 ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa
    26581 ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt tcggacagga
    26641 gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc tttagacatg
    26701 gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa
    26761 gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga cgccgaccgt
    26821 ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat ctaaattaga
    26881 caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc gtggaagctt
    26941 gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg tgatcgaatt
    27001 gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca gtggctgctc
    27061 catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat caatagaaga
    27121 agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag tcaaagatcc
    27181 cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa
    27241 atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc
    27301 tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa ttagaagaat
    27361 ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa ggcttgacag
    27421 aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg cttgaattcc
    27481 gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc cccgaccttt
    27541 cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg
    27601 actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt ataccagaag
    27661 ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc gtgtaccata
    27721 atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac tctgctttga
    27781 aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca accgacaaca
    27841 aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac gtgccaaaag
    27901 gcgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa aacacggggc
    27961 aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat gtcgaaggtt
    28021 gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa atctttacaa
    28081 gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat gtgtacaact
    28141 tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc
    28201 taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa ggtgcacgag
    28261 gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa
    28321 tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt tcaaaagacg
    28381 gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct
    28441 ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat acgatcccat
    28501 tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc tcaaaaatct
    28561 cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa
    28621 tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa tatgcagtcg
    28681 aactcaatcg attgatcagt tatgagatgg aaggcagtgt tgggtaaacc tgttatagga
    28741 gctagaaccc ttgatatata agggttctag cttttttgtt ttgcctgtat actagaaatg
    28801 accagatttt gaccagctct tttactgatg agccatcata tcagcaaagt cttgacctgt
    28861 tttcttaggt gcattatttg taatatgtgc atagatgtct aatgtcactt tactattttc
    28921 atgccctaat cgttgttgaa ctaccttgac agattctcct gcttctaata ataaactagc
    28981 atgtgtatgt ctaaacccat gaggtgtaat acgaggcaag ttatattttt tcaaaatata
    29041 tcttaaccaa tcattaacag tgtttggaat gtatagagtg tttttaactg ttgtaaaaac
    29101 atgttgctct ttagagctag tattgtatcc aagttttaaa tactcttctt tttgctgtaa
    29161 tttccagttg ttgaggatag aaagagtctc agtatctaaa gagataactc gtctagaagc
    29221 tcttgttttc ggagattgaa ttataacttt accaaattca tctacagcaa gagttttatt
    29281 gacatgtagt tctttattaa acgtgtctat gtctttccac tgtaaagcga gtacttcact
    29341 ctttctcata cctgtgtatg ccaataatct gaagaaggcg tattgtttca tgttaccaaa
    29401 atctttaaaa gcatccagta aattattaag ctcctctttt gagtaaaatt tacgattttt
    29461 ctcatactct ttttttcgtg gaagaagtgt tttttccata ggattattgt caatatattg
    29521 cattgcaacc ccgtagcgta aaatttgtgc agtctgtttt ctcatataat cgtaactttc
    29581 atatttactg tgccattcat ctaccacttt ttgacaatat gggacagaga tattcgatag
    29641 tttaagctta ccaaatgctg gaagtatttg attatttgcg tatcttactg agattgcgat
    29701 tgttgaagct ttaacactgg tacgctgatg agttatccat tcattataca ggtcttcaaa
    29761 tgtcatatca ggtgctttct ttttaacacc gtataatatt tgatgtctgt actcgtcata
    29821 atctttttga gcttcttgtt tgctactaaa acctcttctc gtagtattta ttttttcacc
    29881 agttcgtgga tcggtggcaa tgtgtccttt atacatccac gccacttgac cgtttttctt
    29941 tttgtacttt gatatttttg ccattaaatc atctcctctt agtttacaag gagtacttac
    30001 cactagatgg tatcacctcc cttcattttt ttcttagtgt attttttccc aattgatgaa
    30061 atcgtttgtt ctagcttgtt tatattatca gtaatttctc tcacagcttc ttcgggataa
    30121 gacatgtctt tcaattgaat aggtagccat tcatctacca ttctttctag tagttcttct
    30181 atactgctag tgaggaaaag aatgttattg tcaaattctt ctacaatagc attgatgaat
    30241 aattcttcta tttttaagat attgaaaaga ctaatttttt catttttaac tttattacaa
    30301 gtcttgttag caataaattc ttgcatctct ggttctaatt tggaatatct atccagccat
    30361 gtatctaaat tagagtgtac tggtgggaac tgttgacgaa gatgctggaa caggttttta
    30421 tctggaaatg ttataccatc tagtacaatg ctatctctat aaagaataat tcccaaaata
    30481 tattcttcta cattgccaca aactaactcc tctatactca tatttcccaa atcagcaatt
    30541 cgggataatc gctctggtat tggtaaatag gttcccttct cccattcgta gatagttttt
    30601 ttgtcaatta attgaccgtt gttaatcata agaccaaatg ctggttgtgt taatttttta
    30661 ctctttctta tagcttttat ttttgttccg agtatctttt ttcgttctaa ataatttatt
    30721 ggtggacgtt ttatcatatt gaccacacct cctaaaatta gtatacctca aaaaaggggt
    30781 gttgtaaata aattttacaa agctgttgac accccttttt tatcgaggta ttatgtgtat
    30841 gtcgatgaac tggtaatcat tttttgtttg attaatcggt taataacaaa atacttttat
    30901 tttgagcaaa aaatacgaag ataattctta gaaaatttgt tcaagcaatc acgaaaggag
    30961 ggggaaatat gtctctacaa atcattgata gcattgtttc tatacaagtt gaacaacaag
    31021 tgaaagagat aattacagtg ctaaagccta tgctagaaga cattatttct ggagtcactc
    31081 tagagtatat ggactatcag caggcaagtg aatatctagg tgtatcagta ggaacgatta
    31141 gaaaatatgt ttctcaatac gggttacctg ttataaaaat tgatacggtc attagattta
    31201 aaaaatctga tattgatagt ttcatggaac ggtataaagg ttga
//
LOCUS       DADJYG010000014        27633 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.014, whole genome shotgun sequence.
ACCESSION   DADJYG010000014 DADJYG010000000
VERSION     DADJYG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.014"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..659
                     /locus_tag="JZ382_000592"
     CDS             12..659
                     /locus_tag="JZ382_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY2426800.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            743..1687
                     /locus_tag="JZ382_000593"
     CDS             743..1687
                     /locus_tag="JZ382_000593"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAY2426801.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1822..3273
                     /locus_tag="JZ382_000594"
     CDS             1822..3273
                     /locus_tag="JZ382_000594"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAY2426802.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3294..4130
                     /locus_tag="JZ382_000595"
     CDS             3294..4130
                     /locus_tag="JZ382_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAY2426803.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4130..4789
                     /locus_tag="JZ382_000596"
     CDS             4130..4789
                     /locus_tag="JZ382_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY2426804.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4915..5604
                     /locus_tag="JZ382_000597"
     CDS             4915..5604
                     /locus_tag="JZ382_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426805.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5610..6566
                     /locus_tag="JZ382_000598"
     CDS             5610..6566
                     /locus_tag="JZ382_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAY2426806.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6610..7122
                     /locus_tag="JZ382_000599"
     CDS             6610..7122
                     /locus_tag="JZ382_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426807.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7378..7926)
                     /locus_tag="JZ382_000600"
     CDS             complement(7378..7926)
                     /locus_tag="JZ382_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2426808.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8015..9196)
                     /locus_tag="JZ382_000601"
     CDS             complement(8015..9196)
                     /locus_tag="JZ382_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY2426809.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9432..10940)
                     /locus_tag="JZ382_000602"
     CDS             complement(9432..10940)
                     /locus_tag="JZ382_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAY2426810.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11099..11290
                     /locus_tag="JZ382_000603"
     CDS             11099..11290
                     /locus_tag="JZ382_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426811.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11493..12410
                     /locus_tag="JZ382_000604"
     CDS             11493..12410
                     /locus_tag="JZ382_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAY2426812.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12403..13476
                     /locus_tag="JZ382_000605"
     CDS             12403..13476
                     /locus_tag="JZ382_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426813.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13624..14142
                     /locus_tag="JZ382_000606"
     CDS             13624..14142
                     /locus_tag="JZ382_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426814.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14123..14515
                     /locus_tag="JZ382_000607"
     CDS             14123..14515
                     /locus_tag="JZ382_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAY2426815.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14591..15526
                     /locus_tag="JZ382_000608"
     CDS             14591..15526
                     /locus_tag="JZ382_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAY2426816.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15659..16438
                     /locus_tag="JZ382_000609"
     CDS             15659..16438
                     /locus_tag="JZ382_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAY2426817.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16431..16748
                     /locus_tag="JZ382_000610"
     CDS             16431..16748
                     /locus_tag="JZ382_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAY2426818.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16992..18602
                     /locus_tag="JZ382_000611"
     CDS             16992..18602
                     /locus_tag="JZ382_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAY2426819.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18759..19607
                     /locus_tag="JZ382_000612"
     CDS             18759..19607
                     /locus_tag="JZ382_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAY2426820.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19838..20317
                     /locus_tag="JZ382_000613"
     CDS             19838..20317
                     /locus_tag="JZ382_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426821.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20350..22578
                     /locus_tag="JZ382_000614"
     CDS             20350..22578
                     /locus_tag="JZ382_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY2426822.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22707..24350
                     /locus_tag="JZ382_000615"
     CDS             22707..24350
                     /locus_tag="JZ382_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAY2426823.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24423..24968)
                     /locus_tag="JZ382_000616"
     CDS             complement(24423..24968)
                     /locus_tag="JZ382_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAY2426824.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(24991..25476)
                     /locus_tag="JZ382_000617"
     CDS             complement(24991..25476)
                     /locus_tag="JZ382_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY2426825.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25466..26278)
                     /locus_tag="JZ382_000618"
     CDS             complement(25466..26278)
                     /locus_tag="JZ382_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAY2426826.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26417..27529)
                     /locus_tag="JZ382_000619"
                     /pseudo
     CDS             complement(26417..27529)
                     /locus_tag="JZ382_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
BASE COUNT         9047 a         5040 c         5532 g         8014 t
ORIGIN      
        1 ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct ttgtttttag
       61 ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc agtagcgaac
      121 agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg tctagccaga
      181 caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca gctcgtgcga
      241 tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa ttagatagtg
      301 attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa tacgaagcaa
      361 gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta gatcgagatg
      421 aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg atcacgattg
      481 aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca gattgggatt
      541 tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc aatcaaagta
      601 catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa gatgattaag
      661 aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta gacaaaataa
      721 cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc atatctttgc
      781 caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat tagatgaact
      841 ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg aacgaaaagg
      901 aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta tgggacttcc
      961 aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag tgcaggaaaa
     1021 ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa acttggaaat
     1081 gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt tatcttgtcc
     1141 caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg aaacactgaa
     1201 agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt attttgattt
     1261 tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct atgtcaatgc
     1321 tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag tagtcatcaa
     1381 accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg ctttggcgaa
     1441 tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta caggtgggat
     1501 tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt tacagattgg
     1561 taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag aattaacgca
     1621 gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat tacgcaccat
     1681 ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa aaaacactcg
     1741 tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg ggaaaataac
     1801 agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga gcttaaagaa
     1861 aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa gaagcaaaat
     1921 catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca cgagctattg
     1981 attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa aatgctcggt
     2041 caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac acaccgagct
     2101 tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc ttttggacaa
     2161 accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta tggcccagca
     2221 agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc tgctgctgca
     2281 gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc tattagaata
     2341 cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat gccagttggt
     2401 ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac agtttctatg
     2461 agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc gccatatcaa
     2521 gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat agctttttgt
     2581 gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt aaaaaaagct
     2641 gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc actagatggt
     2701 cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc aatgatccgc
     2761 tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc catttcttgg
     2821 actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc tctgcatgta
     2881 tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct attcctttca
     2941 ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga tgcgataaga
     3001 gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt tgtttcttcc
     3061 atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct gactggacaa
     3121 ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg aatccaattt
     3181 atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt tgaagcaaat
     3241 catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga agaatgaaga
     3301 aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct tgcggtacag
     3361 ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa gaattagtag
     3421 tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat gaattagttg
     3481 gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa atctccttta
     3541 aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca gattttgctg
     3601 cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca gagactttca
     3661 aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc cactcacttg
     3721 aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg cgtttagcgg
     3781 aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag tatttaagcg
     3841 atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag aaaatggccg
     3901 tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc aatgaaaacg
     3961 aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc gatggcgtat
     4021 taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat tttcaaactg
     4081 atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga tgtactttcc
     4141 tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag gcttggtata
     4201 cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt tgcttttgac
     4261 agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt atttatcgtt
     4321 ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt taccttatca
     4381 attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg catttattgg
     4441 tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg ctggtttagc
     4501 ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag cttttcgaat
     4561 ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt cgttagcagc
     4621 aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc ggacattcga
     4681 ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta tcgggatcga
     4741 aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa aaatagaaat
     4801 ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct gcctagtatt
     4861 ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga aaacatgaag
     4921 accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga gcaaggcggc
     4981 ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac gcttagctgc
     5041 ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt gacaaaagca
     5101 gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt tgttttacca
     5161 agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt tttagtgtat
     5221 cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat tgcgaaaaaa
     5281 gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt catggcttct
     5341 ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata ttttccaaca
     5401 gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt ttctaacgaa
     5461 cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga gttgcaagaa
     5521 gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca gatccgtcgg
     5581 ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga ggaaaacaat
     5641 ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat aaaatccaac
     5701 cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa ttatttcaaa
     5761 aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc aaattattcg
     5821 atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct gcacagatct
     5881 taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt gttttcgatg
     5941 gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt gattcgaaat
     6001 ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa attgtgatcc
     6061 gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca gatgactttt
     6121 ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct gatttcacga
     6181 tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct atccatctaa
     6241 cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac agtaacgact
     6301 ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa aaatggatca
     6361 tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg gctctttacc
     6421 agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg catcatttgg
     6481 aacttattag tcacctgcta gatcatccaa aagattggct taaatattat tcaaaagttc
     6541 aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga atagaaaaga
     6601 ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt ggtagaaatc
     6661 attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca aaacgggcag
     6721 aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta tgtattaata
     6781 gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc cgtttacaca
     6841 aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca tcgtgtcgct
     6901 ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca tttgttgacg
     6961 attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc taatgaaccg
     7021 atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga atttcctttt
     7081 gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa tcatctgagc
     7141 aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa gaggttgtaa
     7201 cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct caaatttttg
     7261 tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca tcgagaggtc
     7321 gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc gatacattta
     7381 tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat cgtttgacac
     7441 gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt ctacataaac
     7501 cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc gcgattgcaa
     7561 tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat actcatataa
     7621 atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca gtttcttttt
     7681 aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact ctgcaaactc
     7741 atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat cctcttgatc
     7801 tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt ccggctgaaa
     7861 aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata catagcaata
     7921 attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga aaatatttcc
     7981 aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca gtcacaatag
     8041 cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt tttgcgacaa
     8101 gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg atagaagaga
     8161 agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca gtaaaatgga
     8221 aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt ttagtcgcca
     8281 tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag gcaagagggg
     8341 caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg aagatagcat
     8401 tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat
     8461 ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt
     8521 caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa
     8581 tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg
     8641 ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga
     8701 acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt
     8761 gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt
     8821 tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa
     8881 taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac
     8941 ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa
     9001 gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata
     9061 atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg
     9121 cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac
     9181 ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg
     9241 caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca
     9301 ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta
     9361 aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa
     9421 gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata
     9481 tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg
     9541 gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt
     9601 actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa
     9661 tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga
     9721 atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca
     9781 tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc
     9841 acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca
     9901 gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt
     9961 cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa
    10021 cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat
    10081 cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt
    10141 ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct
    10201 actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact
    10261 gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa
    10321 gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc
    10381 aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta
    10441 gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg
    10501 actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta
    10561 ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt
    10621 aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc
    10681 ccataatcac cacggatctt attacgttca atatctccag catagacgca aatcagtatt
    10741 tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga
    10801 agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa atataatttg
    10861 tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa
    10921 tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt
    10981 ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa atggtttaaa
    11041 ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa ggtgctacat
    11101 gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga tttttaccgg
    11161 ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt atctaaacga
    11221 agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa gagatctgcc
    11281 tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc agatggaaag
    11341 actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt atggagcatt
    11401 gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt attaataagt
    11461 gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca aatgcaaatt
    11521 atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa gaatcgtctt
    11581 ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta gaagacttac
    11641 aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt ttatacaatg
    11701 gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc gatcaattgc
    11761 tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa caagtagaaa
    11821 acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat cggcccacgg
    11881 tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat gaagagtggg
    11941 agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag ctagacgcaa
    12001 aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa gaaatcaatc
    12061 gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa atcgaggaag
    12121 aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta ggctggcttc
    12181 cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag caagaagcaa
    12241 gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc agacgctatt
    12301 tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat tatcttggca
    12361 atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga acacgatgaa
    12421 gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa cacttcattg
    12481 gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct agaggaaaca
    12541 aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga aaacacccat
    12601 gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga tcatcaagat
    12661 atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat gagaaaagat
    12721 ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat attgaatgaa
    12781 gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta tgaattactg
    12841 aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac atggaaaaca
    12901 agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt ttcctttgta
    12961 ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa cgtatcacca
    13021 gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg acactggtca
    13081 gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt gaatcaatta
    13141 ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga caatacgaag
    13201 ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt ggatgatatt
    13261 tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga aactacttat
    13321 caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa agatcaaaca
    13381 gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt tatcaattac
    13441 gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga ttaccagatg
    13501 tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa ttgtcacaag
    13561 tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg gatttcatta
    13621 ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg tgtagcaggt
    13681 aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt tttatttata
    13741 gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat tgcgatcagc
    13801 agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta tactgttgtt
    13861 ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct atggttaaag
    13921 ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt ttttgatacg
    13981 ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc gtttgatttg
    14041 atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt caaacgtttt
    14101 tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg caatgagtgt
    14161 cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt ccaaccaaaa
    14221 ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg gtcaaaactt
    14281 ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt tgaaacgaaa
    14341 ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact cacaggatga
    14401 gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta ctggtgccaa
    14461 agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac tataagtttc
    14521 ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt gatgaaagaa
    14581 ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg ctgctctagg
    14641 ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg gcagtgccgc
    14701 catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt tgctgttatt
    14761 cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg aaggacgagc
    14821 aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg gggcacttgg
    14881 ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta atccgatcat
    14941 tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac gcgtggcaat
    15001 gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt tgcgcgaaag
    15061 tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa tcggtttggt
    15121 cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat ttgatgcgat
    15181 cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag caaaagaatt
    15241 ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa tcaagttagc
    15301 ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt taagtccaac
    15361 ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg aagcttactc
    15421 ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga agatgtatat
    15481 atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg tttattcatg
    15541 aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc tacaatttag
    15601 cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag atattttaat
    15661 gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc cactttgtat
    15721 aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg gatttgatcc
    15781 tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt ttttagttgc
    15841 atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt tctttttaga
    15901 attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag aaaagcggac
    15961 gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga actacttaaa
    16021 gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt ggttctcaat
    16081 gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga ttcgagtaga
    16141 tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta tcatgcagat
    16201 gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg tcgtattcat
    16261 ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt catttgccgg
    16321 atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag accgcggtaa
    16381 agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc atgactaaac
    16441 tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg cgcttgttcc
    16501 cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg atgaaatatg
    16561 taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact catgaacatc
    16621 tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc gtggcttaca
    16681 aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt ttgctgtcta
    16741 tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg ttcaaactga
    16801 tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg tagggataaa
    16861 aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta gagagggatt
    16921 tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa agaatagttg
    16981 agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc tttgctaatc
    17041 ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg atggggaaca
    17101 atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct tatggattgt
    17161 tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta gctgtagcca
    17221 caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc aatatcatgt
    17281 ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta ctagccggaa
    17341 tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta tgcccatctt
    17401 tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa aatgatctgc
    17461 ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt atcttggctg
    17521 gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta ttgatcagta
    17581 cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc gtcggacgca
    17641 gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc gaaagagaaa
    17701 accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt gtcggctcag
    17761 tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt cactttttcc
    17821 gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct gtcaatccaa
    17881 ataaattgac attgatcctg atctccatgg taggaacagt agctataagc agcttgccaa
    17941 tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta ggagatagtt
    18001 tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta ttagctggac
    18061 cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc caaatggctt
    18121 tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt caatctttga
    18181 atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa gtcatcttcc
    18241 aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat ggcttagcat
    18301 tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga atagcacctt
    18361 tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt atgcttgcag
    18421 tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca aaaagctatg
    18481 caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta ttttctcaat
    18541 tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga agcaaagaat
    18601 aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg attacgcttg
    18661 tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat atagttataa
    18721 ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca gtagttacag
    18781 atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc gttattccaa
    18841 tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa gcggatgaat
    18901 attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct gtcgtagggg
    18961 aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt ttaagtatcc
    19021 atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa gatgacatca
    19081 gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg ggacacatgg
    19141 tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata cttgcacata
    19201 tcgatcgaat ccgagataat acctacgctt atctgattgt agatgatttg aacaatttag
    19261 tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta aaaatcaaac
    19321 cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt tcaagtaaaa
    19381 aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata caaccgccag
    19441 tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag aaagagaaat
    19501 tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca gtcattggta
    19561 cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc cttttattca
    19621 aaaaacatac caaaaatatt ccaacatcat cttggacttt ctacatgtta ctaatgagcg
    19681 tgtaaaagaa cgagatggtg tttttatttc tatcaatgaa gctctttttc tttgcaatcc
    19741 tcacttcaat cctctataat gtcgcatata gtttagtaaa tgagtaaact actaatttac
    19801 ttatttacta aaaataaaag gatgaggagt taaaataatg gaaataccaa caaatttaaa
    19861 acataaacca gtaatcgttg tagaagacta tgataaagtt gatggaagaa atgcattaaa
    19921 tacggatgca aaagggcttt cactgggcct tgctcagtgg aatgatcgtg gaaaagtcga
    19981 tatctcaggc aaggtatggc gacatactgg agaaaaatgg tcacgacagt cggaagaatt
    20041 accaatcaca cgtattttag atttagcgat cttagctgca caaggaagtt tatacttcca
    20101 agatgcttat cgttatgaaa aattttatga tccagaaaat ccagtcgtag acatcattgg
    20161 attacaaggg aaccgaatga cggtagaagt agataccaaa aatccaaaga tcgatgaaga
    20221 catcattttg tattacgata cactacaaaa agacggagaa ctgattgggg aacgattccg
    20281 tattttaaaa cgtcttttag atgagctagg atattaatag acagagatga tcaaaagggg
    20341 aagagaagca tggagaattt ctcactaaca gaaaaccgaa ccttttcacg gctagaaaag
    20401 caaatgatct ggaagaaacc gaaaagccat cggacaagtg cagaagaatg gcggatcgcc
    20461 tccgaaatca aagggaattg gcaagatcct gaaatgaaaa tcagtaacgt acttttggaa
    20521 ggagatgccg ggtcggggaa gacacaatta gccaaagcac tatctgctga tctgcaactt
    20581 ccctatacaa aagtgacctg ttttgcagat atggacaaat cagatgtttt cggagcctta
    20641 ttacctgtag tagattctaa taaggaacaa gaccaagaat tactagaagc tatttaccaa
    20701 acagatacac ttgaagctgt actgacactg atccagaacc attacggcat tgatcctttt
    20761 tctgctaaag aaaaattagc agagctagtt caaaggatcg aagctgatta tccagatgca
    20821 atccattaca gctattaccc ttctgaaatc gttcgagcga ttgaaaaagg atatttatta
    20881 gagattcaag agccgactgt catccgagat gcctctgttc ttgtggcact taactcagcg
    20941 ctcgaaccaa atgggatgtt gaatttaccg acaggaatca ttcgtcgcca tccagattgc
    21001 gtcattgtga tcactacaaa tcgaaattac caaggaaatc gcccattaaa tgaatcttta
    21061 agagatcgga tgcaacatgc ggaaaaaatg gatcttcctg ccttagaagt catggcagaa
    21121 cgtgcgatgg cgaaaactat gatccaaaaa caagaacttc tcttaaagat ggcagagatt
    21181 atccgtttgt tggatgaaac tgcaaaagct aacgccatca aaggtgtagc aggaatgcgt
    21241 tcttattttt attgggcaaa tacctataag caaggacaag atttactcca gtcgatttat
    21301 ccaaaagttt tgtataaatt atccactgat tcagatgaat tggcaattct tgaacaagct
    21361 ttgtctgaca gtggattgct actggaactt caggacatat tgagaaaaga aaaatggggt
    21421 catcctcaat ctgagcatac aaaaggaaga accattagcg ccgaagaagc aaatgaacga
    21481 aacatcactg aaaatgctga agaaattctg cgcttagatg aaaagaaatc gttgtctgct
    21541 gaagaaccaa atgagggaga agagaaacaa gaagaaacaa tagaaaaaaa cgagattcct
    21601 cctcaaatgc aaaaagaacg cagtagcgat atcgaaggaa aaaatcaaaa agaacaacaa
    21661 acaggagatg attctcaaga aatgtctgct tctgacatgg aagcacatga taaggaaatc
    21721 aagaagcaac tgaataaaga agcgcgacaa atcatgaagg aaacgattca tgaaaaagaa
    21781 ggtctcatcg ttcaccgacc agctttccaa ggaaaaacgc cggaagcacg tgagctacac
    21841 atgcaagtaa tgccaatagt agaatcactt agccggcaaa ttttagagtt actggataat
    21901 gaacaaacag agacgtacca aaaaggaaaa tacgaaggtc agcggttcaa tgcaagcaaa
    21961 gtagcatatg gtgatttgag aaattttgac aaaaagaacc cgccacatga gcaaccttct
    22021 ttagcggtag ctgtacggat cgatgaatct gggtcaatga tacgggatga tcggatcgaa
    22081 gcggcaaaaa aagcagcgat tgccattgcc gaattttcta agaaagtcga tatcccattg
    22141 ttgatttacg gagatacagc agaccgttcg tcaagagaaa agacttcttt gttttcttat
    22201 aaagaatttg aagacggatt tcaatggtta aatgaaaaat tagtcactat gaaaccaagg
    22261 caaaacaatc gtgatggtgc tgcacttcgt ttagcagcag aaaaattaaa cagacaatcc
    22321 gcaacaacca aactgttgct taatattagt gacggtcagc caaaagcgtt gccggattat
    22381 acaggcaaaa aagcaaaaga agatatccag tctgttttga aagaatatga acgtcaagga
    22441 atcctatttg tctccgcagc tattggccaa gacaaagaag aaatcaagtc gatctacggt
    22501 gaaagtcgtt ttgtagatat tactgacttg aatgaatttc ccaagcaaat gattcaactg
    22561 attgcccgct atctatgata aaataaagct caacgaacgc acttagcaga ttcgttgagc
    22621 ttttcgtttc tagttaccgg taacaaggat acttctaaaa tagaatcggt tataatcaag
    22681 agggaccgtt aaaggagtga aacgatatga tacaagaaca aaaatggtgg aaagaagcag
    22741 taggctatca aatctatcct gctagtttca aagatagcaa tggcgatggt caaggagaca
    22801 tcaatgggat ccgtgaaaaa cttaattatt tgaaagatct tggtatcggc tttatctgga
    22861 tcaatccgat ctatcaatct ccatttgtcg ataatggtta tgacgtaagt gactatcaag
    22921 atatagaaga gagattcgga acgatggaag attttgattt attgctaaaa gaagcacatg
    22981 aattagggat caaagtgatt atggatcttg tcatcaacca tagttcggat cagcatcaat
    23041 ggtttgaaga atcgaaaaaa tctaaagaaa gcccgtatcg cgattattac atttgggtcg
    23101 acggggtaga tggcaaagaa ccgaataatt ggacatcgat tttcggcggt tctgcttggg
    23161 agtattctca tgaaactgga cagtattatc tgcatgtttt tgcaaaagaa caaccagact
    23221 taaattggga aagcgagaaa ttgaaagaag aactattcaa tatgatccga tggtggttgg
    23281 ataaagggat agatggattt cgtttagatg cgatttctca tgtaaaaaaa gatgaatatt
    23341 cagtaaaagc cacagagaac cctttctcac cctttcaaaa tgtctcagga atcgaagaac
    23401 atttgaccga tttgaaacat gtttttgaag agtacgatat catgactgtc ggagaagcaa
    23461 gtggtgtcac tgcagaagaa ggaccacaat gggtagggaa agacggttat ttcgatatga
    23521 tattcgaatt tgatcatatc catatctggc agcaagaaaa agaagggcaa ttagatgtcc
    23581 ttaaattgaa acttgcatta agcgcttggc aaacttcatt ggatggtatc ggatggaacg
    23641 ctttatatat ggaaaaccat gatgttcctc gtgccgtttc agtgtttgga gatactcgtc
    23701 ccgatttttg ggctatgtct gcaaaagcaa tcgctatgat gtatttcttt ttgcaaggta
    23761 cgccatttat ttatcaagga caggaaatcg gaatgacgaa tatgcccttt gaatcgatcg
    23821 atcaagttga tgcagtagat tcgaaaagac tttataaaag gctgcttgcg gaaggtaaga
    23881 caagagaaga agcattggat atcattcgtg aaacaacgag agacaacagc aggacaccca
    23941 tgcaatggac ttcggaacaa tatgctggtt tttctacaca tgaaccttgg ctgatcacta
    24001 atccaaatac caaaacgatc aatgtagaac aacaagaata tgaaccagag tcggtcctgc
    24061 aattctataa gaacatgatc cgcattagac aaaccaataa aggtctgata tacggttctt
    24121 ataaagaata tcttcatgaa catccacaac tttatgtcta tgaaagatat ttagaagatg
    24181 aacaatatct tattatggta aacttaacag aatcacttgc tgactatgaa cttccaaaag
    24241 aagcagatca gtcctggaca ttactcttgt caaattcctc tagcggagaa tttgaagcta
    24301 aaggcatact agcaccttat gaagctcggt tatataaaac aaacaagtaa aacttcctcg
    24361 ttaaagttca ttagttggac tttgcgagga agtttttcag attgctctgt caattactca
    24421 tttcaataat tcgctctagg accaccgata tatttaggac gagagcgaag ggctgtcacg
    24481 tgagcttgtc caagggtttt caaacgaggt ctgacaggta cttggacatg tttgatatgc
    24541 ataccgattg atgtgtcacc gatatcgata ccggcttgtc caactacgtg ttccacttcg
    24601 acaggatcat tgaactggtc aaaagcagca acagaacaag cacctccagc atgtagtgca
    24661 ggaacaacag agacaatttc gaaatttttt gcttctgcca ctgttcgttc tactactaat
    24721 gctcggttca aatgttcaca accttggaca gctagcgcaa tttctttagg atccaacaac
    24781 tcttttaacg tacggatgat ccattgacca acttccgcac tggaattttt tccaatatgt
    24841 cctccaacga cttcgcttgt gctgcatcct aaaacaaaaa gatcaccttt tttcaagtgt
    24901 gcctcagcca atatgtcatt aactatttca gtcaattggt cttttaattc tttttctgac
    24961 aacttcattt tctaaaacct tctttttcaa ttaatattgc ttccgtgctt gaatacgttg
    25021 aagacttgca gttataggaa aggcaagtcc cattccaatc agattttgga agatgtttcc
    25081 tggtatagaa gccactccag ccggccaatc atataaaatc caaccggcga taccatagcc
    25141 gatgatcata actaagcttc ctaataaaag ccctaatccc cacatacgag tagaagattt
    25201 gctgaaagaa ccagcaacat atccttgtaa cccgtggatc actaatgaaa aaactgccca
    25261 ctgcggatat cctgaaatca aatcgatcaa tagaccactg gaagcaccaa caactaatcc
    25321 acccgttgga ccataaagaa aagcagcggt atagatcccg gcctcgcata atgtcacaaa
    25381 tccattcgta gccggtacag ggatcaaaat tgttaatgaa agggcaactg tcaacgcaat
    25441 cagcatagca tagatcgtta attgcttagt tttcatttct cattcctcct ttttgaatac
    25501 cgccagacca gacacttccg gtatgatcgt tcatcagcac gccattagaa atacaatggg
    25561 caacaaattt tttactttct gctactgctt tggtaaaatc tgcccctaac acaagattcg
    25621 ctgttattgc agaagaaaag gcacaaccgg ccccgtttac tgttgtagtg tcgatttttt
    25681 tatgagaaaa tacctcataa tgatcaattg aatagagaag atccacagct tcatctccag
    25741 ccatccttgt gccgcctttg atcacaatgt tttttgttcc tgaagcagtg agttctttag
    25801 cgagatggat catttctgtt aacgaagagg gaagagcttg accagaaagt aaagcagctt
    25861 ctgccaaatt aggcgtaatc atcgtaacaa gtggaaaaag ttcttcgatg agctgcgaaa
    25921 tatacaattg attgatcaca gcttcagttt ctttgaatgc caaaacaggg tctaaaatga
    25981 ttgggatatt ttgttttgtt tttatgaagt ctcgaacaac catgattgct tcacttgaat
    26041 tcaataaccc aattttgata ccatctaatg aagtcatttt atcaatcgaa actaactgtc
    26101 gtttaagcaa ttccaaagat atagattcca gcataaatcg ctcattaata tctagtgccc
    26161 ctatagaagt caaggcgctt agtccgaata gaccaaaatt ctcaaaagtt tttaaatctg
    26221 tttggattcc tccaccagca aatggatcag aaccgccaat tgtcaagatt cttttcataa
    26281 cattttcctc catgaataag agtataacga aattttcaaa aaagaaaaac agccaattgg
    26341 catattttta tctcatccaa tttaaaaaaa caaaaagaaa agaaagacca aaatggatct
    26401 ttctttcctt aaatagttag gacttttgtg attttaattc tttctcgatt tgaagccgtt
    26461 gcactttcat tgtagctgtt cgcgggattg catcaaaatc acggatgata gggacgttca
    26521 agcgaggaag ttcatggact tgctcccacc atcgattcca atccatttct ttatcaggag
    26581 ctaaagcaat aatcggttgt ggagaatgat ttttatccct tacaatcact acttctgcca
    26641 aaaaatctaa cgaatctaat aaaaagtctt caatagctaa tgtactattg atcgtttcga
    26701 tcaaatccac ttgccgatct ttcaaaaata ggtggccatg cttatccata aaaccgtaat
    26761 ctccgctatc ccaccaatcg ccataaacat tttcctggaa ccgtgcatct tccttgtaat
    26821 aagtcaaagc ccgtccttta gacagtaatt gtatatgacc atctgtgttt tcaggtagga
    26881 cattcccaac tgaatctgta atccgagcgc ttgtcatgtc ttccagtcca acgcccatat
    26941 ctcttgcatc acttgttttc aaactctcta gtgtatgagc cttaagaatc attggtccgc
    27001 attcactttg gccatatact tgaagaaata ccgcatcctg tgccatcgac gttcgcaaaa
    27061 atgtcttcat tgtctggttg tttatagcat caaaagtgga gtgatagtat cggatacccg
    27121 aaaaagcttc tggatgctcc ttagcagtaa aacgccactg aacaaagtta ttcggatggg
    27181 tctctagtgc aattggccgg tgagcttcca acatatgaac aacttttgaa ctttgtgcgt
    27241 ttgctaaagg catcatagga aatcccatgg acatcaaaga agagatacca atattgaatc
    27301 tggaatgcac tggagagatg tgaaaagcga ttaatttctt ttccgaaatt tttgtgaaaa
    27361 ctgttttttg ccatttcgtc cgccatccca tagaatgagc agaatgacaa ataagtttag
    27421 ggataccagt tgtaccagaa gtatgggtca tataagaaat ttcatttttg tctaaaaatg
    27481 attgagaaac agacaaagca ggttgttcca ttaaataaag aataggaata cccgtggtgc
    27541 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
    27601 acttaataga acactgtaag ccagtaatat gct
//
LOCUS       DADJYG010000015        27612 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.015, whole genome shotgun sequence.
ACCESSION   DADJYG010000015 DADJYG010000000
VERSION     DADJYG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.015"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(24..203)
                     /locus_tag="JZ382_000620"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="JZ382_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="JZ382_000621"
     CDS             complement(407..766)
                     /locus_tag="JZ382_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY2426827.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="JZ382_000622"
     CDS             complement(796..1137)
                     /locus_tag="JZ382_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426828.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="JZ382_000623"
     CDS             complement(1134..1808)
                     /locus_tag="JZ382_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAY2426829.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="JZ382_000624"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="JZ382_000624"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY2426830.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="JZ382_000625"
     CDS             complement(4322..5512)
                     /locus_tag="JZ382_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY2426831.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="JZ382_000626"
     CDS             complement(5533..6060)
                     /locus_tag="JZ382_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY2426832.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="JZ382_000627"
     CDS             complement(6107..8896)
                     /locus_tag="JZ382_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY2426833.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="JZ382_000628"
     CDS             complement(9043..9243)
                     /locus_tag="JZ382_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY2426834.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="JZ382_000629"
     CDS             complement(9696..10016)
                     /locus_tag="JZ382_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426835.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="JZ382_000630"
     CDS             complement(10519..11637)
                     /locus_tag="JZ382_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426836.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="JZ382_000631"
     CDS             12108..13415
                     /locus_tag="JZ382_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAY2426837.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="JZ382_000632"
     CDS             13536..14735
                     /locus_tag="JZ382_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY2426838.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="JZ382_000633"
     CDS             complement(14762..16030)
                     /locus_tag="JZ382_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAY2426839.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="JZ382_000634"
     CDS             complement(16200..16841)
                     /locus_tag="JZ382_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAY2426840.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="JZ382_000635"
     CDS             complement(17250..17753)
                     /locus_tag="JZ382_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2426841.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="JZ382_000636"
     CDS             complement(17876..18640)
                     /locus_tag="JZ382_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAY2426842.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="JZ382_000637"
     CDS             18748..19023
                     /locus_tag="JZ382_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY2426843.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="JZ382_000638"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="JZ382_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY2426844.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="JZ382_000639"
     CDS             complement(20579..20980)
                     /locus_tag="JZ382_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY2426845.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="JZ382_000640"
     CDS             complement(20994..21803)
                     /locus_tag="JZ382_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2426846.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="JZ382_000641"
     CDS             complement(21790..22680)
                     /locus_tag="JZ382_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426847.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="JZ382_000642"
     CDS             complement(22693..23172)
                     /locus_tag="JZ382_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2426848.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="JZ382_000643"
     CDS             complement(23348..24289)
                     /locus_tag="JZ382_000643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAY2426849.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="JZ382_000644"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="JZ382_000644"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAY2426850.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="JZ382_000645"
     CDS             complement(25274..26470)
                     /locus_tag="JZ382_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY2426851.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="JZ382_000646"
     CDS             complement(26488..27216)
                     /locus_tag="JZ382_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2426852.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         8018 a         5894 c         4785 g         8915 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aa
//
LOCUS       DADJYG010000016        27234 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.016, whole genome shotgun sequence.
ACCESSION   DADJYG010000016 DADJYG010000000
VERSION     DADJYG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.016"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(116..310)
                     /gene="ssrS"
                     /locus_tag="JZ382_000647"
     ncRNA           complement(116..310)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JZ382_000647"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(382..1668)
                     /locus_tag="JZ382_000648"
     CDS             complement(382..1668)
                     /locus_tag="JZ382_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY2426853.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKELQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVK
                     HALKDKERGLGQQAIQLDEEALLHLSRATNGDLRSALNGLELAARSTPPDKEGKIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAERLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGNVPDHLRDSHYKGAKSLNRGVGYQYPHHFDHAWVNQQYLPDR
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPLKN"
     gene            1806..2477
                     /locus_tag="JZ382_000649"
     CDS             1806..2477
                     /locus_tag="JZ382_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAY2426854.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPATMIEQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KIPKKIDALPRIGIPNKGEWTEKPLRFVVSGNPYISLQKKNQIEEDWGWRKENEKEGS
                     INILR"
     gene            2443..2931
                     /locus_tag="JZ382_000650"
     CDS             2443..2931
                     /locus_tag="JZ382_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAY2426855.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDEQNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQKLAELVEAKQQNEIETYLPYP
                     SY"
     gene            2944..3891
                     /gene="prmA"
                     /locus_tag="JZ382_000651"
     CDS             2944..3891
                     /gene="prmA"
                     /locus_tag="JZ382_000651"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY2426856.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLSLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEVAKDVHVSANDLLKGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMIEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            3892..4656
                     /locus_tag="JZ382_000652"
     CDS             3892..4656
                     /locus_tag="JZ382_000652"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY2426857.1"
                     /translation="MQRYFLKESYEPKDHFILKGETYHHIVRVMRMEPKDEVFLAFND
                     QIAVVAEITKITEDAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWVIQKSTELGA
                     QAFIGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKRDLFER
                     LDDYDVVLVAYEESAKQGEKSNVAKILSETKPGSRILAVFGPEGGLSHEEIDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMDPSHS"
     gene            4888..7101
                     /locus_tag="JZ382_000653"
     CDS             4888..7101
                     /locus_tag="JZ382_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY2426858.1"
                     /translation="MPKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEVTLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     NGTVEDIRVATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQSEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPEKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRMNG"
     gene            7113..7559
                     /locus_tag="JZ382_000654"
     CDS             7113..7559
                     /locus_tag="JZ382_000654"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY2426859.1"
                     /translation="MRAVIQRVSQAAVSVDQQEIGKIGCGLLVLLGIHETDTPKDVEY
                     LVKKIAQMRIFEDDQGKMNISVEDVGGQILSVSQFTLFADTKKGNRPSFVEAARPEIA
                     IPLYESFNEGIRSRGITVATGQFGADMSISLVNDGPVTIIIDSKNK"
     gene            7730..8989
                     /locus_tag="JZ382_000655"
     CDS             7730..8989
                     /locus_tag="JZ382_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAY2426860.1"
                     /translation="MKEVPVIDVKITDLKRLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAIVIYGSSAAKIFYDPRNFKRKGAMPKLVLKTLFGQDGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALETQHGQFKLFDLSKMVFFTSICEWAGINLSLLSKDEV
                     KKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEDSWVILDLYGTDHDERTVEAPDS
                     FMIKRYLGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9077..10024)
                     /locus_tag="JZ382_000656"
     CDS             complement(9077..10024)
                     /locus_tag="JZ382_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY2426861.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRIVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDKSTPKNEAVAALHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHREAEKELFPYLEKNKISFVPYFPLASGLLTGKYER
                     GQEKQFGEGDPRKSNPDFQGERFKEILAAVDILRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSSEDYQTIDEAFHGF"
     gene            10419..11711
                     /locus_tag="JZ382_000657"
     CDS             10419..11711
                     /locus_tag="JZ382_000657"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY2426862.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEIRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKDTRQAYRQALIDYLLPFEAELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VADAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPTFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            11725..13494
                     /gene="aspS"
                     /locus_tag="JZ382_000658"
     CDS             11725..13494
                     /gene="aspS"
                     /locus_tag="JZ382_000658"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY2426863.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQVVFNPEINKEAWEIADKCRSEYVIEVTGVVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFEMELIDLSETVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAEPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKAEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLKTLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            13603..14478
                     /locus_tag="JZ382_000659"
     CDS             13603..14478
                     /locus_tag="JZ382_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2426864.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWFKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            14745..16628
                     /locus_tag="JZ382_000660"
     CDS             14745..16628
                     /locus_tag="JZ382_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY2426865.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKSILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLF
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIAFVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VSE"
     gene            complement(16703..16987)
                     /locus_tag="JZ382_000661"
     CDS             complement(16703..16987)
                     /locus_tag="JZ382_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAY2426866.1"
                     /translation="MANKHYFYVLFCKDQTFYGGYTTDLSRRLGEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKEHYLKTHSTVLLPENR"
     gene            complement(16977..17711)
                     /locus_tag="JZ382_000662"
     CDS             complement(16977..17711)
                     /locus_tag="JZ382_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAY2426867.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSQVFSFSLDAVLLAHFSRVPKKG
                     QIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLDEQMTVHTIDLK
                     DSLSVIRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDNSSKLLK
                     TNGRFAMVHRPERFLEIIQIMQNHRIAPKRIQFVYPKAGKEANILLVEGIKEGKLDGF
                     KVAPPLITYDADGEYTAEIREMLYGK"
     gene            17840..18463
                     /locus_tag="JZ382_000663"
     CDS             17840..18463
                     /locus_tag="JZ382_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAY2426868.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRKRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEIKKK"
     gene            18651..19772
                     /locus_tag="JZ382_000664"
     CDS             18651..19772
                     /locus_tag="JZ382_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAY2426869.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVALYNELMVDWNLKEGIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFNNIQFFFLPYFEPIAARLYFEDDSIRTIQQAVAKVIDKMEEL
                     FDSGKKHVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLATDLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIQIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKHKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            19779..22904
                     /locus_tag="JZ382_000665"
     CDS             19779..22904
                     /locus_tag="JZ382_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAY2426870.1"
                     /translation="MKPKKLKLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPEEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSEKETVLRHLFGTQLFQQFNERLKEKAKQQQQKLDHLEQNLHLLQKRFV
                     LVKEEEAVAGFEAVLSQWTNHQAILEKQITKEKEVLVDYQEKQKQLENSYYRFETLKK
                     SYEEKATLLNKQKKLAAQKDEIEEKKRWIGYYEFTQRLIEPIQHLKEIKAEKIELSQK
                     SNEQTKKLAMTTQHYEEWQKNETDRIHRQTKIKEAAQQLQNKQMMVPIVEALRSKKQE
                     AEQLKHSLDKEETNAEKLHQKQAIYQERTRFLQEILLDQEKLQEDRLAFGQLKYLDEK
                     QQEIKQQKTKSIESLEQIREELKKAAEETTKNNQRLKQQIEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPVQQSKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKNE
                     AHENNLQQQLSDLEQKIMETNIQLAGQEKHLQQALQQAFPAITEQTTTALTVVEQSLD
                     KRKSKIANARAELEEISQQVEKIEQQLAVYKQQQTAKNNQLQQLQGEIKSLEKQLQDT
                     DSNCLEEEIRQLEKQLEEDSNQLAAEQKAGMQLLQDLHMFRTQLDLQQEQIEKTDEKI
                     EKEQQRIAEKLETQAYFSTTEEVCAFAQQQAQYEENQRIITTYHEAQLVTADRLRQLE
                     NIQLTEIVPDTEEISQQLEEIREMIETKQEKIYSLQEQKINNQKIYDECLSIYHASQT
                     QLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFELA
                     DTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGVA
                     IDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTEG
                     SGQSKIRYQLG"
     gene            22923..26117
                     /locus_tag="JZ382_000666"
     CDS             22923..26117
                     /locus_tag="JZ382_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY2426871.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKTTS
                     EGEIIAKGFSRLKTQKNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILAHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINGQNDQGIQFSDQNKPLSF
                     KVEKQEEDYLLQALNDFDFFFMHYQWGLIQGVFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VIFKKKELPALFKEIIPLLSEIGDVVVASEVEKEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTNPKHEVSANQDFQVLRDRAEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYTFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPQIEVTETGSWLDIRFDVSGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRSEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQNLIHPEGYQAQLPKGLNAKMREYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERESFSALIVAPASLIYNWQAEVKKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLADYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEISSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEK"
     gene            complement(26263..26970)
                     /locus_tag="JZ382_000667"
     CDS             complement(26263..26970)
                     /locus_tag="JZ382_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAY2426872.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDEDMHPLYIRFR
                     HHSDENFPKVMAGEWTLEYMRAHRISQSLKDLDYPHITEEEGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSHNMLVSQATGFQKP
                     EKEIFQLAEKEFHMLPGESLYVGDNYDNDVLGAKSADWQALWFNHRERKIDQTPICDI
                     EISSFDQLLETMKTIFA"
BASE COUNT         9085 a         4851 c         5862 g         7436 t
ORIGIN      
        1 aatagtaaag tggcgataat catcaaaatc ctaatcatga acaaatcctc caaaataagc
       61 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
      121 ctccgttaat gccgttattt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
      181 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
      241 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
      301 acagatttcc catctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
      361 gaaatagaac cgttttcttg tttaattttt taggggatgt tttttccact cttttatgcg
      421 gtaatactgt tgacctagcg cctgttcata ttttcctgta tcctttggct gataatattg
      481 agcatttttt aacctatctg gtaaatattg ctgattgacc caagcatgat cgaaatgatg
      541 aggatactga taacccacac cgcggttgag ggatttcgca cctttataat ggctatctct
      601 caaatggtcg ggaacattac cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
      661 tgccatatag gcagaattgg attttggcga caagcaaaga tcaacgacga catctgctag
      721 cgggatacgt gcctcaggta accctagtcg ttcagcagct aatacagcgt ttactgtacg
      781 cgcagcagca gcaggattgc ctaaacctat atcttcataa ccgataacca tcaggcgacg
      841 acaaatactt gccagatcac ctgcttcaac tagacgggcg agataatgaa gggcagcatc
      901 cacatcactt ccgcgaatcg atttttgaaa cgctgaaata acgtcataat gagcatctcc
      961 atttttgtca tgggtaagag cttttctttg gacacattct tcgataatcg acagagtaag
     1021 atgaatcttt ccttctttat ctggaggagt cgatcgtgca gccagttcca acccattcaa
     1081 agcactccgc aaatcgccat tcgttgcacg agaaaggtga agaagagcct cttcatctaa
     1141 ctggattgct tgttgaccaa gaccgcgttc cttatccttt aaggcgtgtt tcactgccaa
     1201 ctggatgtct tgctctgtta atggcttgac ttcaaaaatc tgtgtgcggc tgcggatcgc
     1261 tggattgata gtaatataag gattttcagt agtcgctccg atcaagatga ttctgccact
     1321 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
     1381 taagaggatc acagtaccac tcattttagc ttcttccgct acaacctgca gctctttttt
     1441 tgaatctgta gccgcgttca gcatgcgaaa tgcataatta gtagaaccgg caatcgcact
     1501 tgcgatactc gtttttccag taccaggagg cccatataaa atcatggaag aaagcatacg
     1561 agcatcgacc atgcggcgta tgatttttcc ttcaccgact agatgttgct gaccaacgac
     1621 ttcatcgatc gttcgaggtc gcatgcgata agccaatggc tgttgcatag gattttcctc
     1681 cttcttgttt ttacgttcct taattataac atatttagta atatagctag aaagaacagc
     1741 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
     1801 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccggaag
     1861 tagcgcggga tttattgggg atgtatttgg agcatgacac acctgaagga agattagctg
     1921 gatatattgt agacgcggaa gcatacttag gtccagaaga cgaggccgcc catagctatg
     1981 gtttaagaag aacaccacgt gtgcgggcaa tgtatgaaaa accaggtaca atctaccttt
     2041 acacgatgca tactcatcgg attttgaaca tcatcactca gccagaaggg attcctcaag
     2101 gagtaatgat acgtgcaatt gaaccagcta caatgattga acaaatgagt aaaaatcgtg
     2161 gtggaaaaac tggtccagat atcagcaacg gtcctggaaa attagtggaa gcattagcaa
     2221 ttcctcaaga actctacgga caatctatag cagatagttc attacgttta gtttttgaga
     2281 aaaagaagat accaaaaaaa atcgatgctt tgcctaggat cggtatcccg aacaaaggag
     2341 aatggacaga aaagccgttg cgttttgttg tgtctggcaa tccatatata tctttacaaa
     2401 aaaaaaacca aatcgaggaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
     2461 ttaacatact tagatgaaat aatcgaaaaa caagtgacgg actatgatgt ggctttagat
     2521 tgggatacaa aaaatcatac gatcgaaatc gttgttcgtt tgtttgcgga aaataaagca
     2581 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
     2641 gatggcgtac tcctttttaa tcctcagaaa tctgtttttg atgaacaaaa ttatctagcg
     2701 gtaatcccct atgaaggaaa aaaaggattg gcaaaatcag tagctgattc tttagtggaa
     2761 tatttgaatg aagtattagc acaagggcaa agtgatttgc ttgatttttt agatgaagat
     2821 gatgacgaag ctgtattcga actccattgg gataatcaga aactagcaga gttagtagaa
     2881 gcaaaacagc aaaatgaaat agagacttat ttaccgtacc caagttatta aggagtgaca
     2941 gacatgaaat ggaatgaagt aaaagtagaa accgaaagtg aagcagtcga agcggtcgct
     3001 aatattttga tggaggcagg agcgagtggt gttgcgattg aagactcttt agatgttgaa
     3061 cattttcaat cggatcaata tggtgaaatt ttagataaaa atgattttac atcgttgaaa
     3121 gaaggcgcgc tagtcatggc ttattttcca gaaacgactt ttttaccgga aatcttgcct
     3181 tttatcaaag atcgtatcac gcaattgcct gagtttggtc tagcgattgg aaaaaacaaa
     3241 gtaactgtta gcgaagtaga ggaaagcaat tgggcaacag catggaagaa atattatcat
     3301 ccggtgcgta ttacacgaat gctgacggtt gcgccaagct gggaaaacta tcagacagaa
     3361 gatcctctag aaaaaatcat cacattagat ccaggcatgg cgtttggaac aggcacccat
     3421 ccgacgactt ctttgtcatt gacagcacta gaaacagtat tgcgtggtgg cgaaacattg
     3481 ctagatgttg gaactggttc gggtgtacta agtattgcag caaaatatct tggtgcgaaa
     3541 gatgtctatg cctttgattt agacgaagta gctgttcgtt cagcgaaaga aaatatggat
     3601 atgaatgaag tagctaaaga tgttcacgta tcagctaatg atcttttgaa aggaatcgag
     3661 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatcccg
     3721 gatgcatggc gtttactaaa acaaacaggg acattgatcg tttcaggaat cattgaagag
     3781 aaaaagcaaa tggttctaga tgctatgata gaacaaggat tcatcgtgga tcaaattttc
     3841 caacaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcga
     3901 tattttttga aagaatcgta tgaaccaaaa gaccatttta ttttaaaagg ggaaacctat
     3961 catcacattg tacgggttat gagaatggaa ccgaaagatg aagttttttt ggcattcaat
     4021 gatcagatag ccgttgttgc agaaatcaca aaaatcaccg aagatgctgt ttatctgaaa
     4081 gaaattaaaa aagaagaaaa ccaaaaggaa ctgccaagtt cgataaccat agctagcggc
     4141 tatcctaaag gagaaaaatt ggattgggtt attcagaagt ccactgaatt aggcgcacaa
     4201 gcttttattg gttttccggc atcagcctct gtggttaaat gggatgcaaa gaaacgtatg
     4261 aagcgacaac aacggcttga aaagattgct caagaagcag cggaacaatc ccatcggcaa
     4321 atcgtgccct cagtagagtt actggaaacg aaacgtgact tatttgaacg gttagatgat
     4381 tatgatgtag tcttggttgc ttatgaggaa tcagcaaaac aaggcgaaaa atcaaatgta
     4441 gccaaaattc ttagtgaaac aaaaccgggc agtcgaatct tagctgtctt tggaccagaa
     4501 ggcggcttgt cccacgagga aatcgattat ttcgtagaac acaaagggat actatgtgga
     4561 ttaggaccaa gaatccttcg tacagaaaca gcgcctcttt atttattaag tgctgctagc
     4621 tatcaatggg agttaatgga tccctctcat agctaacatt tttcatcaat aaaagagtat
     4681 cgacggaaag aattgaaaaa atagggaaca atttgtataa tagaaacaat tatttgtaca
     4741 atgaatacga aaaattgttt gaaaaagctt tttaaaagcg aacatttccg taaaaaaatg
     4801 tataacttca cggattttgg ggcaactatc aaacagatat ttgtcccgat tcctgtttta
     4861 ccgaaaaaga aagggggatc gattcctgtg ccaaaagaag aaattttaac gggtcccggt
     4921 gtcataaagc tcgttagtca atatatgggg cccgaacatg tagcatttgt tgagaaagca
     4981 tgcgaatatg cgactgctgc acatgacgga cagtttagaa aatcaggaga accctacatt
     5041 atccatccta tccaagtcgc aggtatatta gcagatttaa aaatggaccc ccacacagtg
     5101 gctactggct tcttgcatga tgttgttgaa gatacagaag tcaccttaga agatctgaga
     5161 gaagaatttg gcgatgatgt cgctatgtta gtagacggag taaccaaatt agggaaaata
     5221 aaatacaaat cccacgaaga gcagctagca gaaaaccatc gaaagatgct gctggcaatg
     5281 gctcaagatt tacgcgttat catggtcaaa ttagctgata gactacataa catgcgtacg
     5341 ttgaaacatc tgcgagaaga taagcaaaga aggattgccc aagaaacttt agaaatctat
     5401 gcacctcttg cacatcgttt aggtatcagc cgaatcaaat gggaattaga agatacagca
     5461 cttcgttatc ttaatccaaa acagtattac cgtatcgttc atttgatgca gacgaagcga
     5521 gaagaacgtg aaaaatacgt gaatggtact gttgaggata ttcgagtagc aacagaagag
     5581 ctagggattt ttgcagagat ctatggacgg ccaaaacata tttattcgat ctatcgtaaa
     5641 atgaaagacc agaaaaagca attcaacgaa atctatgact tgctagccat tcgagtaatc
     5701 gtagattcaa tcaaggattg ttatgctgta ttaggtgcga tccacacaaa atggaaacca
     5761 atgcctggca gattcaagga ctatattgcc atgccgaaag ctaatatgta ccaatctttg
     5821 catactacag tgattggtcc ggcaggaaat ccagtagaaa tccaaatccg tacacaggaa
     5881 atgcatgaaa tcgctgaatt tggggtggct gcgcactggg cgtataaaga aggaaaaaat
     5941 gaaaaagtag aaccagatgg tatgacgaaa caattaagtt ggttccatga gatacttgaa
     6001 ctccaagatg aaagctatga tgcctctgaa tttatggaag gtgtaaaagg agatatcttt
     6061 agtgataaag tctatgtctt cacgccaaaa ggagacgtta ctgagctgcc aaaaggatct
     6121 ggaccactgg actttgcata cagtatccat acagatatcg gtaataaaac cactggtgca
     6181 aaagtaaatg gcaaaatggt gcaacttgat tacaaattaa aaaacgggga tatcatagaa
     6241 atcatgactt ctccaaattc atttggtcca agtcgtgact ggttgaaatt ggtggcaact
     6301 agcaaagcaa gaaataagat caagcgtttc ttcaaagcac aagatcggga agaaaatgtg
     6361 atcaaaggtc acgagtctgt ggtcaagtgt attacagatt tagggtttac acctaaagat
     6421 attttgacaa aaaacaaact tcaagaagca cttgatcgtt ttaactatca atcagaagat
     6481 gatctatatg cagctgtagg atatggggaa gtcagtcctt tgacaatggc caatcgtctg
     6541 acagaaaaag aacggaaaga acaaaaaatc gagcagcaaa agcaagaagc agaagaaatc
     6601 atgaatcagc cgaaaaaaga acctgagaaa atgaaagtac gtcatgaagg tggcgttgtc
     6661 attcaagggg tagaaaactt actgattcgt atcagccgat gctgtaatcc gattcctggt
     6721 gatgatatcg ttggctacat cactaaaggc agagggatat ccatccatcg tcgagattgt
     6781 ccgaatgttc agcctgataa accaaatgta gcagaacgtt tgattgaagt cgaatgggaa
     6841 gatacatcga atacacgaaa agagtacgat gcagacttgg aaatctacgg ctataaccgt
     6901 tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc tgacgaaaaa tctcaacagc
     6961 gtcgaagctc ggacgaataa agataaaatg gcgacaatcc atttgacggt tggtatccag
     7021 aatttgtccc acctaaaaag tatcgtggat aaaatcaaag cagtacctga tgtctacagt
     7081 gtacgccgga tgaatggata ggagggccag atatgagagc agtcattcaa cgggtgagtc
     7141 aagcggcagt gtcagttgat cagcaagaga ttggaaaaat tggctgtggc ctactcgttt
     7201 tattaggtat ccatgaaaca gatacgccaa aagatgtgga atatctagta aaaaaaatcg
     7261 cacaaatgcg tatttttgaa gatgatcaag gaaaaatgaa tattagtgta gaagacgtag
     7321 gaggtcaaat tttaagtgtc tctcaattca cactatttgc ggatacgaaa aaaggaaatc
     7381 gaccaagttt tgtggaagcc gcacgaccag aaatagctat tcctttatac gaatcgttca
     7441 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
     7501 cgatttctct cgtcaatgat ggacctgtaa cgattattat tgattctaaa aacaaataaa
     7561 gtcagttggt tttcatcaat aagaaatata aatcaagtcg tttaagttag actgaaaaac
     7621 tctaacttaa gcgacttttt tctcgtctat aaaaagcgct tcttttgtca gcagtcagga
     7681 aaaaagttaa gcttaaatta tacaagttat ttttgggagg ttgctgaaga tgaaagaagt
     7741 accagtaata gatgtcaaaa tcacggatct aaaaagattg tatcaaaagg gatacaatat
     7801 gttggaggag ttacgtcatg aagcagacgc tcccgttgtc aaagcaaaga ttttcaataa
     7861 agaagctatt gtgatctacg gatcgtcagc ggcaaaaata ttttatgacc caagaaattt
     7921 caaacgaaaa ggtgctatgc caaaattagt gttgaagaca ctttttggtc aagatggagt
     7981 acagactttg gatggagctg cccatcatca tcggaaaaat atttttatgg atctgatgac
     8041 ccccgaaaga atggaagatt atcatcgtat cttagataag aacctgacgc aagcacttga
     8101 aacacagcat ggtcaattta aattatttga tttaagcaag atggttttct ttacatcgat
     8161 ttgtgagtgg gcaggtatca atttatcttt gctttctaag gatgaagtca aaaaattagc
     8221 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
     8281 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcggca
     8341 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
     8401 agatggtcag cttttgcctc tggaagtagc agctgttgaa ttattgaaca tcatccgtcc
     8461 aacggttgct ctaactgtgt gggcggcgtt gatgggacac gcgcttttca gcagacctga
     8521 tttgtaccaa caattgaaaa acgacttttc tacgttgcaa gatccgttca ttcaagaaat
     8581 gagacgctat tatccgttct ttccgatgtt acctgcgatt tctctgaaag aagtagaagt
     8641 cgatggttat cgtataccag aagatagctg ggtcattcta gatttatatg gaacagatca
     8701 tgatgaacga acggttgaag caccagactc ctttatgatc aaacgatatt taggaaaagc
     8761 aaaagatatt tcatataaag aagaatatga gatgattgcc caaggtggag gaaactttcg
     8821 acaaatgcat cgctgcgcag gagaatggat cactctccac agcctacgtg tgttttctga
     8881 tcagctagta aacaagtttg aattctctgt acctgaacaa gattggacga tcccattcaa
     8941 tcagtttccg acttatccaa atagccgagc gctactttat aagaattaga ttagtataaa
     9001 cttatcgaaa aaaggaagat cagcactgtt tgatcttcct tttttcatac tactataagt
     9061 ggataaggtc aattgctcaa aatccgtgaa atgcttcatc aatggtttga taatcctcac
     9121 tggataaatg caaatctaaa gcttggacat tatcagagac ttgctctgga cgtttagctc
     9181 cgggtatgac tacagacact cttggattct tcatatacca ggctagaaca agttgcgcag
     9241 gggtggcttg gtatctttta gcaatggggc gaagtatgtc gacagcggct aaaatttctt
     9301 tgaaacgttc tccttgaaaa tccggattgc tttttcgagg atctccttcg ccgaattgtt
     9361 tttcctgacc acgttcatat tttcctgtca gtaaaccgga agcaagagga aaatagggga
     9421 caaagctgat tttgtttttt tcaagataag gaaataattc tttttcagcc tcccgatgga
     9481 tcaaactgta tttatcttct actacatcga catatccatc tgcattcgct tctttcaatt
     9541 gttctaacgt gaagttcgat acaccaactg ctcggatttt tcctgcttct ttcaattcat
     9601 gcaatgcagc aactgcttca ttttttggtg tgcttttatc cggaaagtgg atataaaaaa
     9661 tgtcgatgta atcagtttgc agacgtttca gtgcgtcttc caccgattgt ttgagaaatt
     9721 cgggtgaatt gtcaaatgtt cgtcctttat ttggcacatg agcagctttt gtagcaatga
     9781 cgatacgtga acgatcatat tctttttcct tcaatacctc accgatcagt tcttcagaac
     9841 ggccgtttcc ataagcatag gcggtatcga tcaaattgat tccgctattc aaagctgtac
     9901 gaaccacttg ttttcctgtc tcgtcttcca aaccgtcaaa aagattgtgt cccccaacgg
     9961 catttgctcc tagtccaagc tgttctgcgt agacttgtga atgtccgatt cttatttctg
    10021 ccatagctga ttcctccttt ttttctagtc taaaacaaaa gaacaaaaag ctgaactaaa
    10081 atgtttttgt gagggaaaat agtcagataa ctcttgactt tgagattggt ttttagtagg
    10141 ttataagtaa tatcgaaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    10201 aggatcatcg aggctgaaaa tgatccaaag tttcattagc ccaaagacac tctggagatg
    10261 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    10321 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcaagatga acaagggtga
    10381 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    10441 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    10501 gtttattgtt cagagattat cagtataatg aaatacgtac accgattttt gaacattatg
    10561 aagtgatatc ccgcagtgta ggtgatacga cggatatcgt ttcaaaagag atgtatgatt
    10621 tttatgacaa aggggaacgt catgtgacgc ttcgaccaga aggtacagcg ccaatcgttc
    10681 gttcttttgt tgagcataaa ttgttcggtc cagagtatgc aaaaccgttc aaaacttttt
    10741 atatgggacc aatgtttcgc tacgaacgtc cgcaaaaagg acggttacgt caattccatc
    10801 agatcggagt agaagcattt ggatcagaga atcctgcaac agatgtggag agcatggtga
    10861 tggcactcga tttcttcaaa cagttgggaa tcacgcaaat ccgtctagtc atcaattcat
    10921 tgggtgataa agacacacgt caagcctatc gtcaagcatt aatcgactat cttttacctt
    10981 ttgaagcaga gttgagtgaa gactctaaac gccgtcttca cgaaaatcca ttgcgtgtgt
    11041 tggatagtaa ggacaaacga gatcaaaaat ttgtagcaga tgcgccatct atcttagatt
    11101 atttaagcga acctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    11161 ctgttcctta tgaaatcgac agtaatatgg ttcgcggatt agattactat acgcatacga
    11221 tttttgaaat catgagtgac gcaccgaaaa tgggggcaca agcaacgatt tgcgcaggcg
    11281 gacgttatga caatctagtg gaagaactgg gtggtccttc aacacctggc tttggatttg
    11341 caatgggtat cgagcgatta ctcttgacta tggaagcaga ggaagtagtt atccctacat
    11401 ttaatgagtt ggatgcttat gtggtcgcac tcggagacga aacaaatatc gaagcattga
    11461 aagtggtcca agcgattcga aacttcggtt tttcagctga tcgcgacttc atgaaccgta
    11521 aagcaaaagc acaatttaag acagcagata aagcgaatgc caagttagta ttggtaatcg
    11581 gtgaagatga gcttgctagc caaacaatca atgtcaaagc aatggcaagc cgaaaagaaa
    11641 aaagttttcc attagaagaa atatatgagc attttgatga agtctatgat gaaatgactt
    11701 tattcgagta ggaggaaaaa agaaatggca caaagaacga tttattgcgg attagtttcc
    11761 gaacaacaat tagaacaaac agttacatta aaaggatggg tacaaaaaag acgagacctt
    11821 gggggagtaa tctttatcga tctacgggat cgtgaaggaa tcgttcaagt cgtcttcaat
    11881 cctgaaatca acaaggaagc atgggaaatc gcagataaat gtcgtagtga atacgtgatc
    11941 gaagtaactg gggtagtcag aagacgcggt gaaaatgcag taaatcctaa aatggccact
    12001 ggtgaatttg aagtgattgc aagtgaaatc gtgattttga atcaagcaaa aacaccgcca
    12061 tttcaaattg aagatgatca agcaatcagt gacgaaatac gtttgaaata tcgttatttg
    12121 gatctgcgac gtcccaaaat gactaaaaac atcaaattga gaaatgcgac aacaaaagcg
    12181 attcgtcatt atttagacga taatgacttt ctagatattg aaacacctta cctaggaaaa
    12241 tctacaccag aaggcgcaag agactactta gttccttcac gtgtccatgc tggacatttc
    12301 tatgcgttgc cgcaatctcc tcaactattc aaacaattgc tgatgaatgc tgggttcgac
    12361 cgttattatc aaatcgttcg ttgtttccgt gacgaagatt tacgtggaga tcgtcagcct
    12421 gaatttacgc aagtagatat tgaaacaacg ttcctaacag cagaagaaat ccaaacttac
    12481 acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg gcatcgaagt cacattgcca
    12541 tttccccgta tgacctatga tgaagcaatg gctcgctacg gcagcgacaa accggataca
    12601 cgttttgaaa tggaattgat cgatctaagc gaaacagtca aagaagtaga attcaaagtc
    12661 ttccaaatgg ctcttgaaaa cggcggagtc gtgaaagcat tgaatgcgaa gggcgctgca
    12721 gatcgctact cacgaaaaga tatggatcag ttaggacaat atgtagggca attcggagcc
    12781 aagggacttg catggctgaa agtggaagaa gacggtctga aaggcccgat tgctaaattc
    12841 atgggagaag caacagaagc aatcatcaaa gcaacagatg cagaaccagg tgacttgctg
    12901 atgtttggcg cagataaaag tgaaatcgtc gctgctgcat taggggctat ccgaacacgt
    12961 ttaggaaaag aattaggact gatcgatgaa tcaaaattca atttcttatg ggtaactgat
    13021 tggcctcaat ttgaattttc agaagaagaa ggtcgatatg tctctgctca ccatccattc
    13081 acgatgccaa aagcagaaga tatcccatta ctggctgaag accctgcaaa agtctatgca
    13141 gaagcttatg atatcgtctt gaacggttat gaactaggtg gaggttctat ccggatccat
    13201 actcgggaac ttcaagaaaa agtgttgaaa actttaggct tcacaaaaga acaaatggaa
    13261 gaacaatttg ggttcttgct aacagcttta gattacggat tccctccaca tggcggcatt
    13321 gcattaggat tggatcgttt tgcaatgtta ttagcagggg aagacaatat tcgtgaagtc
    13381 attgcctttc ctaaaaacgg aaaagcagcc gatgtgatga gtgatgcacc aagtatggtt
    13441 tcacctttac aattgttcga attgaatatt gatgtaacca atatagaaga atagccgata
    13501 aaaaggcaaa aaggatttgt cgaatctgct tcttttggca accttgattt aattgaaaaa
    13561 cactttataa tgaagccata acatataaag cggggctttt ccatgaaaaa aacattcgaa
    13621 aattgggctt accatgatta cacgaccaaa ctttcggtat caatcgttta tgcgttgatt
    13681 gcatctgtcg cattgaattt cttttatcaa ccaggacata tttattcaag tggtattacg
    13741 ggcttggctc agattttgac gactttgtca aaatcactga tagggttcga ggtgccggta
    13801 tcaaccactt tgtatttact gaatatacct ttgttcgtct tggcgtggtt caaaatcggg
    13861 aagaagttta caattttcac ttttatcaca gtcactttga cctccctttg tatccatatt
    13921 ttaccggaaa cagtcttatc taccgatcca atcatttgtg cgttgtttgg tggggctgtg
    13981 atgggactag ggattgggct agcgttgaga aatggcctat catcgggtgg tctagatatc
    14041 gtgagtatca cgattcgtaa aaaaacagga aagtcgattg gttctatctc gatctacttc
    14101 aatgcattga tcgtttttgt tgctgggatt ctatttggct ggcaatatat gttttacagt
    14161 acgttgtcta ttttcgtcag tggaaaagta actgatgtcg tttatactaa acaaaagaaa
    14221 atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag tgaatgaaat ccaaaactgt
    14281 atgcgtagag gaatcacgat tatcaatgaa gcagaaggtg cttataaaca tgataagcag
    14341 acagtattga tgacaatcgt tacacgttac gaactgcctc tactcagaga agcgatgaaa
    14401 gcttctgatc cgcaagcttt tgtaagtatt gcagaaaatg tccagatact tgggcgtttc
    14461 tacgaggatg gcatttagat cgagtttgac aaaaattcat gaaatactac tgtagacaga
    14521 cattcgtttg tctgcagttt ttttctacca aaggatggat cagcaaatag aaactacttt
    14581 tttatttgaa attttcttaa agttgaaaaa aagcatgttt attttttaaa atttatggga
    14641 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    14701 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    14761 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggttg
    14821 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    14881 tttttttcgt cgtatctggt tatctgatca cggatctgct aaggcaggaa tgggaacaaa
    14941 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    15001 tcgtttttat gcttgttaca gcttcggctt atatcacctt gttccagcga ggattgttga
    15061 ataatcttag aggagttgtc gtcagcagct tactgtatgt caacaattgg tggcaaatca
    15121 ataacggttt atcttatttc gaccgatttg ctaatgaatc accgtttaca catatctggt
    15181 ctttagcggt agaaggtcaa aactatttgg tttggccaat cttgtttgta ttgttgatgg
    15241 tatttgttcg taaaagaaaa tggatctttt ataccgtgtt aggtacatct ttcctctctg
    15301 cgatcttgat gatgattttg tatacaccag ggggcgatcc aacccgtgtt tattatggaa
    15361 cggacacccg gctgttttct atatggttgg gaagtgcgct cgcttttgtt tggccaagta
    15421 ctcgtttgaa gaaaaacatc ccgaaacaag caaaaagtat cctgaatatc gctggaggaa
    15481 tctcactgct ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    15541 attatggagg gatgctccta gtgagtctgt tatgcgtagt actagtcgca gtgacagcac
    15601 atcctggagc aagcttgaat cgttggctga caaatccagt atttacttgg attggcaaac
    15661 gaagctacgg gatttatctg tatcagtttc cagttatgat tttttatgaa gcaaaagtga
    15721 ctgatattgc tgatcatgtt ctgttccata ctttagtaga aatcacattg attttaatca
    15781 tcagtgaact ttcttatcga tatgtggaac gtccgctagc acgttttgac tatggtcgta
    15841 cattccaagt gatgaaagga tggttcagta aaccaatcat cagtaagagc aaaccatggg
    15901 ttgttccagg cacattactt gtagcaattg cattcgttgg tttcgttacg gcaccgaaaa
    15961 atgctgtaac ggccgatcaa aaacaattgc aggcaaagat cgctgaaagt aaaaaattag
    16021 cagaagaaac cagaaacaat tcagaaaata ccactagttc cgttgaccaa gcgattcttg
    16081 ataagtacga gttgtctaag gctcaaggaa acaaggcaca aaaattagag ttaacggcat
    16141 ttggtgattc agttatgcta gatgctgcaa ctgatctaca agagctttat ccaaaagtag
    16201 tggtagatgg cgatgtgggg cgacaattat acgccagtgt accgtatatt gaaaaattaa
    16261 aggaacaaga tttattgaaa gacactgttc taattggact aggaacgaat ggctcattct
    16321 ctgaagctca gtttgatagc gtgatggatg caatcggtga tcgaaaagta tactgggtca
    16381 atgtccgagt accgacgaaa cgttggcaaa atgacgtgaa tgcgatgctt gaacagatga
    16441 caaaaaaata cgacaatatg acgctgattg attggtatag ctacagtaat gaccatagtg
    16501 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcacc
    16561 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagcgag acaaacgtat
    16621 cagaataata gctacaaaaa acttcaggta aaaacagttg tttttgcctg aagttttttt
    16681 atatttatcg tgtttttaac agtcatctat tttcaggaag caaaacagtt gagtgtgtct
    16741 tcaaataatg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    16801 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    16861 gacgtgtata tttagcgcca gtaccattgt tatgttcccc tagtctccta gaaaggtctg
    16921 ttgtgtaccc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgtttat
    16981 ttgccataga gcatctcacg aatctctgct gtgtattccc cgtctgcatc gtaagtgatc
    17041 aatggcggag cgactttgaa tccatctaat ttaccttctt tgattccttc aactaacaaa
    17101 atatttgcct cttttccagc ttttgggtaa acaaattgga tccgtttagg tgcaatccga
    17161 tgattttgca taatttggat gatctctaag aatcgttcag gtcgatggac catagcgaaa
    17221 cgcccatttg tttttagtaa cttacttgaa ttgtcgatca cttcttctaa tgttgtatgg
    17281 atttcatgac gggcgattgc taggtgttta ttagggttcg ttttatttgt tggcagccct
    17341 ttgaaataag gtggattgca gacgactaag tcacaagaat tatgccgaat gacggataag
    17401 ctgtctttca gatcgatggt gtggactgtc atctgttcat ctagatggtt cagcgcgatg
    17461 ctacgtcttg ccatgtctgc taggcgaggc tgaagttcga tagcatcaat agctgcttga
    17521 gttcgtttgc ttaaaaacag gccaactgca ccattacctg cgcaaaggtc aacaatctgt
    17581 ccttttttag gcacacgaga gaagtgagca agtagcacag catctaaaga aaaagaaaat
    17641 acttgggaac tttggataat tttgatatct tctgcgtata attggtcgat tcgttcgtct
    17701 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtttgtatta tactattttt
    17761 attaaaaaga accaaggtgg cttgtcaaac ctctgttttt tatgcatact atgaccataa
    17821 gaagggaaag gaagagacta tgttttatcg atttatgcga ggggtagttc gagtactatt
    17881 agctttagtc aatgggaatg cccgttatga gaataaagaa gtattaccgc aagatgagaa
    17941 ttacatacta gttgcccccc atcgtacttg gtgggaccca ttatatctag cggtagctgc
    18001 agcaccgaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cggttctgcg
    18061 atttatatta actcgatcta atgcttttcc agttaaaaga gagaatccag gaccaagcgt
    18121 cattaaaaca cctgtaaaga ttttaaaatc gacaaattta agtttaatca tgttccccag
    18181 tgggacaagg catgctacag aacttaaagg cgggatggcg ttgatttcca gaatgtctaa
    18241 agcgaaaatc gtaccagccg tataccaagg tccactgaca cttggggatt tgttcaaacg
    18301 taagagagta acagtgcgtt tcggtgaacc aatcgaccta tcagatatca agaaaacgga
    18361 taaagaagga atggcggaga ttgaacgacg aacacaagcg gcttttgaca aattggacaa
    18421 agaagtgaac ccagatttta aatatgaaat caaaaaaaaa taaagtgata agtttaggag
    18481 tgggcggtct ttttgaatct cctgttctgt tcagactttt cgatgaaaaa cgagggagag
    18541 acgtcaagtc tgccctcttt ttgctttcta acgtgcaaaa tagaagacag gtgtcatgta
    18601 atggatcaaa gtgttataat gattagaagg acattttttg aggtgattgg atgcgttttt
    18661 tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg attgaagacc
    18721 aacgtcatgt attatcttct atactggata tcgcgaaaga agaaaaagta gaggcaatcg
    18781 tgattgcagg agacttatat gataggagtg tgcccgctgt cgatgccgta gcattataca
    18841 atgaattgat ggttgattgg aatttaaagg aagggatccc aatttttgct atatcaggaa
    18901 atcacgatag cagtacgcga ctttcagctg gtacgccatg gttcagttat tcgaactatt
    18961 ttttgcgaac gcagctagca gaagctttcg aaccagtcga gtttaacaac atacaatttt
    19021 tctttcttcc ttactttgaa ccaattgcag ccagattata ttttgaagat gacagtatcc
    19081 gcacgatcca gcaagcggta gcaaaagtga tagataaaat ggaggagctg ttcgattcag
    19141 ggaaaaaaca cgtattgatt agccattttt ttgtaatggg aagtgtaaaa tctgattcgg
    19201 aaacgaaaat cgaagtaggg ggtctggatt cgatcccagg agaactactt acaccgtttg
    19261 attatgtagc actaggacat ttgcataatc aagcagcttt aagacaggat aatgctcgct
    19321 acagtggctc gccgttgaaa ttttctctat ctgagatcaa ccagaaaaaa ggcgtatgga
    19381 tcgttgacct ggcaacagac ctagagttga attttaggga gatcgaacca ctgcatgata
    19441 ttgaagaaat aacagcaagt tttaaagaat tgttggatcc tgccttctat cagtccgtca
    19501 atcgggagaa ttatatccag atctatctca cggacagaac gatcatcccg aatatgatga
    19561 accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac ggccgagcag
    19621 aacaaacaaa gcacaaacga aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag
    19681 cattttttag tgaaatgacg gaagaacagc taacggcaaa ccaagaaaaa tggctcggag
    19741 aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa aaattaaaac
    19801 ttaaaaattt tggtcctttt atcaatgaga ccgttgattt ttcacgattg acagaagcac
    19861 cgcttttttt gatcagcggg aagacaggag caggaaaaac gacgattttt gatgggatca
    19921 ctttcgcact ttttggtgaa acttccggaa gacttcgttc tggaaaagag atgcgttcct
    19981 tgtttgctac accagaagaa gaaacaagcg tgactttttc ttttgaacat cagcagatga
    20041 gctatgaaat cgaacgaaaa ccagagcaag ttttagcgaa acgaaaagga aacggaacaa
    20101 aaaaacaggc agctaaagta acgttgacca tttttgatgg gaaaggaaaa gaaataaaac
    20161 aaatcacaaa acgtacagaa gcagaccagt tgatcagaga actgatgaat ctcgatgcga
    20221 aacagttttc ccaaatcgtt ttactgcccc aaggagaatt ccgtaatttt ttggtttcat
    20281 caagcagtga gaaagagact gttttacgtc atttgttcgg tactcagctt ttccaacagt
    20341 tcaatgagcg attaaaggaa aaagcaaagc aacagcaaca aaaactagat catttagagc
    20401 aaaatcttca tcttctgcaa aaaagatttg ttttagtcaa agaagaggaa gcagtagcag
    20461 ggtttgaagc cgttctttcc caatggacaa accatcaggc catactagaa aaacaaatca
    20521 ctaaagaaaa agaagtttta gtagactatc aggagaaaca gaaacaatta gaaaattcct
    20581 actatcgttt tgaaaccttg aaaaaaagct atgaagaaaa agcgacatta ctaaataagc
    20641 aaaagaaatt agccgctcaa aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt
    20701 atgaatttac acaacgctta attgaaccaa tccagcacct gaaagaaata aaagctgaaa
    20761 aaatagaact gtcacaaaaa agcaatgaac aaacaaagaa actagcgatg actacgcaac
    20821 attatgaaga gtggcaaaaa aatgaaacag accgcataca tcggcaaaca aaaatcaaag
    20881 aagctgctca acaattgcaa aacaaacaga tgatggtacc gattgtcgaa gccttgcgta
    20941 gtaaaaaaca agaagcagaa caattaaaac attcattaga taaagaagaa acgaacgccg
    21001 aaaagctaca tcaaaaacag gctatttatc aggaaagaac tcgatttctt caagaaatct
    21061 tattagatca ggaaaaactg caggaagatc gtcttgcttt tggacagtta aaatatctag
    21121 atgaaaaaca gcaggaaata aaacaacaaa aaacaaaaag catcgaatca ctcgaacaga
    21181 taagagaaga gctcaaaaaa gcagccgaag aaacaacaaa aaataatcag cgattgaaac
    21241 aacagataga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc
    21301 ttcagctttt tttgcgtccg ggggaaccat gtctggtttg tgggtcgaca gaacatcccg
    21361 tacaacaatc aaaacatgaa cagccaaata aagaagatat cttgaaagta gagcaacaac
    21421 tggcagaagc tgagtcagct gtagaaagaa cccagcaaat cattgccaaa aatgaagcac
    21481 atgaaaataa tcttcaacag caattatctg atttagaaca aaaaatcatg gaaacgaata
    21541 tacaactagc tggacaagaa aaacacttac aacaggcttt acaacaggca tttcctgcga
    21601 taacagaaca aacaacaact gctcttacag tagtcgaaca atctttagat aaacgaaaaa
    21661 gcaagatagc caatgcaaga gcagaactag aagaaataag ccaacaagta gaaaagattg
    21721 agcagcaatt agctgtctac aagcagcaac agacagcaaa aaataatcag cttcaacaac
    21781 tgcaaggcga aatcaaaagt ttagaaaaac aattacaaga tacggatagc aactgtttag
    21841 aagaagagat acgacaactt gaaaaacagt tagaagaaga ctcgaaccaa ctcgctgctg
    21901 aacagaaagc aggaatgcaa ttgttgcaag atctgcatat gtttcggaca caacttgatc
    21961 ttcaacagga acaaatagaa aaaacagatg aaaaaataga aaaagaacag caaagaatag
    22021 ctgaaaagct ggaaacccaa gcgtattttt caacaactga ggaagtctgt gcattcgcac
    22081 aacagcaagc acaatacgaa gagaaccagc gcataatcac tacttatcat gaagcacaat
    22141 tagtgacagc tgaccgacta cgtcaattag aaaatatcca gctgacggag atagttccag
    22201 atacagaaga gatcagccag caattggaag agataagaga gatgatcgaa acaaagcaag
    22261 aaaagatata cagtttacaa gaacaaaaaa tcaataacca gaaaatctac gatgaatgtc
    22321 tttcgattta tcacgcaagc cagacgcaat tagatgaact tacgcaatta cagcaacttt
    22381 cgcaaacatt caacggagaa aatcctaaga aaactagttt agaacggtat gtgcttcaag
    22441 tttatctaca agaagtgtta caagtagcaa atgatcatct ccaacgttta acgaaaaatc
    22501 gttatcagtt tgaactagcg gatacgatcg gcagttatcg agggaaaacc ggattagaaa
    22561 tcaatatcta tgatgatcag gcaggcatgt ctcgaagcgc gcatacgctg tctggcggtg
    22621 aaagttttat tgcagcactt tctttagcat tagcccttgc ggatgtgatc cagacacaag
    22681 ctggtggggt agcaatcgat gcattgttta ttgatgaagg ttttgggtca ttagatgaag
    22741 aagctttgga aatggcgatg gaagctttag aaacgattga aagtgaagga agaatgattg
    22801 ggatcattag ccatgtaaga gaattgaaag aacgagtatt acagcaaata agaattgata
    22861 cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg agattggtaa
    22921 ctatgaaatg gagtattccg gaaaaagtca ttgcacgtgg gcgtcaatac ttgcaagaag
    22981 gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca gaagtcttgg
    23041 gcagtgaatt gtacatggtt gatttggatg ggacagcaaa agagatagat tactgccaat
    23101 gtccatattg ggaggaacat aaatattgta agcatacagt tgctgtggaa ctttacttac
    23161 ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc gatgttagaa ccggtaaaaa
    23221 caacaagtga aggagaaatc attgcaaaag gtttttctcg tctaaaaaca caaaaaaatc
    23281 ctcaagcggt gcaacccttg attattgaat accaagtaga aacgattgag acaaatcagt
    23341 atcatccaga gctttctatt ttggcgcttt acttacgtat cggttctttg gcatcgccga
    23401 aaaagacata tatcgtaaaa aacatctatg attttttaca ggcttacaca gaaaaagaaa
    23461 cctacacagt caataagcag tatcagtttc gtctagataa acgggctttt caaaaagaag
    23521 atcaaaaaat actcgctcac cttgcaggaa gtgcccaaac gcatcaatta ttaggcacga
    23581 atggcttgca agtcaaaggg aaactagata aaagatacct gcttctccca gtagaacaag
    23641 cccgcttcct gctagaacag atgaatcaga cgacacgttt gcatctgcaa ataaatggac
    23701 aaaacgatca aggaatccaa ttttccgatc aaaacaagcc tttatcattc aaagtggaaa
    23761 aacaggaaga agactattta ttgcaagcat tgaatgattt tgattttttc tttatgcatt
    23821 atcagtgggg acttatccag ggagtattct attctctaac gaaatatcaa caaacagttt
    23881 atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtaata ttcaagaaaa
    23941 aagaactacc ggccttgttt aaagaaatca ttcctcttct ttcggagatt ggagacgtag
    24001 tggttgcctc tgaagtagaa aaggaagtag cggatattcc agtcgaattt attttctttt
    24061 tccgaaaagc caaaggaaaa atccaagctc gagtcgattt cgtttatggc gaagtcatct
    24121 attcgaccaa tccaaaacat gaggtctcgg ccaatcagga ctttcaggtg ctgcgtgaca
    24181 gagctgaaga gaaacgagtc cttgatttat tcaggatgta tcgctaccag aaaaatgaaa
    24241 ctggctatga gcgtccgttg ccaagcgggg aggaactgta tacctttttc cgtacagaac
    24301 tatctgtttt ccgtcagttt ggtgaagtca gacttggcag aaagttgcgg gagttgtatt
    24361 tagatgcgca taaacatcaa ccgcaaatcg aagtaaccga gactggttca tggcttgata
    24421 ttcgattcga cgtatcgggg atagaagagc aagagattga tcatgtactg caaagcttgt
    24481 tgcgaaacga tgctttttat acattggaaa acggacaggt tctttctttt gattcagagg
    24541 aattccaaca aacaagccaa gtattgcaac aattacggga gtcaatacgt tcagaagaag
    24601 ggacgatcca tgtacctaag aatcaaggac tgatcattca aaatcaattg gaaaaaagca
    24661 atgctacttt tagcgaatcg tttcaaacga tggtccaaaa tctcattcat ccagaagggt
    24721 atcaggcaca acttcccaaa ggattgaatg caaagatgag agagtatcag aagcaagggt
    24781 tccgatggtt gaagatgttg ggccattatc agtttggcgg tatcttagcc gatgaaatgg
    24841 gacttgggaa aacactgcag acgattgcct ttttactttc ggaaaaagaa gaacgggaaa
    24901 gcttttctgc tttgatcgta gcgccggcca gcttaatcta caactggcag gcagaggtaa
    24961 aaaaattcgc tccttctttg agtatccaag taatcaatgg gaataaaaaa gaacgggaag
    25021 aattgctggc taaagacaca gatattcgag tgacctctta tgccagtttg agacaagatt
    25081 tagctgatta ccaatcacaa aaaattgatt atttgatcct tgatgaggcg caaatggtca
    25141 aaaatagtag tacgaaaact gctcaagcat tgagagaatt agctgtgcct cagcgatttg
    25201 cattaagtgg gacaccaatc gagaataatt tagaagaatt gtggtcgttg tttgcaacaa
    25261 tcatgccagg atttttccca accaaaacaa aattcagaga acttgccgca gaagaaattg
    25321 cgcaaatgat ccgaccgttc attttacggc gagataagca aactgtgttg aaagacttac
    25381 cagaaaaaac agaaatgaat ctatattctg cactgacaga agaacaaaag accgtttatt
    25441 tagcttactt gcgtcaaatg cgtgaagaaa tctcgtcaat ggattctgaa gcattcaaga
    25501 aaaaccggat cgggatacta gcaggtctga caaggttgcg tcaaatttgt tgcgatcctc
    25561 gtctgtttat tgaagattac caaggcggtt ctggcaaact cgaacaagta aaagatctat
    25621 tagtcgctgc caaagagaac aaacgcagag ttctgctgtt ttctcaattt acaggtatgc
    25681 ttactatctt acaagaagag ttagccgagc tggggatctc aacattttat ttaagaggca
    25741 gtacaaaacc gcaagatcga ttaagtatgg tagatgcatt taatgcaggc gaaaaagatg
    25801 ttttcctgat ttctttaaaa gccgggggaa cgggactgaa tttgacagga gcagatacgg
    25861 tcatcttgta tgacctttgg tggaaccctg caattgaaga acaagctgct ggtcgtgcgc
    25921 atcgaatcgg tcagaaaaat gtggtagaag tgtggcgtat gatttctgaa ggaacgattg
    25981 aagaacgaat ggattcgctc caacaagaaa aacgcgaact gttccaaaaa gtgatccaag
    26041 gaaatgaaga acagctgaca aaactgactg aagaagatat ccgcatgatc ttaagtgtag
    26101 gagagattga gaagtaagag attcgaaaca atcgttcagt aatggataag aagatctttc
    26161 gaaaaaagca ggtgtttagt tgatttaaga aatagcaagg aagttgggtt tccgttttaa
    26221 cccgatttcc ttgctattat tttttgtttt tatttatagg agttaagcaa agattgtctt
    26281 cattgtttct aacagctgat caaaagaaga aatttcgata tcgcaaatag gagtctggtc
    26341 gattttgcgt tctcgatggt tgaaccaaag tgcttgccag tcggcacttt ttgcacctag
    26401 aacatcattg tcataattgt cgccaacata tagactttct cctggaagca tatgaaattc
    26461 tttctcagca agctgaaaaa tctctttttc tggtttttgg aaccctgttg cttgagaaac
    26521 cagcatgttg tgggaaggaa tccaattgtt caattgaagt tgttttaatt tttttgtctg
    26581 atggtcagtc ggtccatttg tgataattcc taatgggaca tttttttcct tcaagaagtc
    26641 aagtgttttt ttgacctcct catgcaaaca gatattatca agttcttcct cgtaaatttt
    26701 ttgaaatagg agaccttctt cttctgtgat gtgagggtag tctaaatctt ttagtgattg
    26761 gctgattcga tgtgctcgca tatattctag cgtccactct cctgccatta cttttggaaa
    26821 gttttcatca ctatgatgac ggaagcggat atatagtgga tgcatatctt catctgaaac
    26881 aagcggcacg actcttttta ccgcgtttcg aaatggctgc tgctgatcat acattgtatc
    26941 atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt tttcattgat
    27001 tttttcacaa atccttgact attgtaacag aaaaaattat ctaaaaaagc tgataaataa
    27061 aggtttttct tactatattt ctgtttcctt gacagaataa ggtttttaga tttcagtttt
    27121 tttcactaaa aatcatgaaa cacgtcaata ggtttgatga acaaattaat cattattgtg
    27181 taactcggtg ggtttatttt aatgcaatct ctgaggaatt ttcgaaaggt ttat
//
LOCUS       DADJYG010000017        26606 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.017, whole genome shotgun sequence.
ACCESSION   DADJYG010000017 DADJYG010000000
VERSION     DADJYG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.017"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1152
                     /locus_tag="JZ382_000668"
     CDS             <1..1152
                     /locus_tag="JZ382_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426873.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="JZ382_000669"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="JZ382_000669"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY2426874.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="JZ382_000670"
     CDS             2728..3003
                     /locus_tag="JZ382_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAY2426875.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="JZ382_000671"
     CDS             3009..3776
                     /locus_tag="JZ382_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAY2426876.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="JZ382_000672"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="JZ382_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAY2426877.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="JZ382_000673"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="JZ382_000673"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAY2426878.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="JZ382_000674"
     CDS             7920..8222
                     /locus_tag="JZ382_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAY2426879.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="JZ382_000675"
     CDS             8225..9388
                     /locus_tag="JZ382_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAY2426880.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="JZ382_000676"
     CDS             9414..12842
                     /locus_tag="JZ382_000676"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAY2426881.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="JZ382_000677"
     CDS             12863..13987
                     /locus_tag="JZ382_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426882.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="JZ382_000678"
     CDS             13977..14414
                     /locus_tag="JZ382_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAY2426883.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="JZ382_000679"
     CDS             14398..14736
                     /locus_tag="JZ382_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAY2426884.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="JZ382_000680"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="JZ382_000680"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAY2426885.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="JZ382_000681"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="JZ382_000681"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY2426886.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="JZ382_000682"
     CDS             15893..16936
                     /locus_tag="JZ382_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAY2426887.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="JZ382_000683"
     CDS             17095..17823
                     /locus_tag="JZ382_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAY2426888.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="JZ382_000684"
     CDS             17939..18184
                     /locus_tag="JZ382_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAY2426889.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="JZ382_000685"
     CDS             18181..18399
                     /locus_tag="JZ382_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY2426890.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="JZ382_000686"
     CDS             18535..19074
                     /locus_tag="JZ382_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAY2426891.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="JZ382_000687"
     CDS             19067..21340
                     /locus_tag="JZ382_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY2426892.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="JZ382_000688"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="JZ382_000688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY2426893.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="JZ382_000689"
     CDS             complement(22513..22767)
                     /locus_tag="JZ382_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY2426894.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="JZ382_000690"
     CDS             complement(23167..23436)
                     /locus_tag="JZ382_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426895.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="JZ382_000691"
     CDS             complement(23906..24361)
                     /locus_tag="JZ382_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY2426896.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="JZ382_000692"
     CDS             complement(24499..26427)
                     /locus_tag="JZ382_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAY2426897.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4610 c         5615 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagag
//
LOCUS       DADJYG010000018        26078 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.018, whole genome shotgun sequence.
ACCESSION   DADJYG010000018 DADJYG010000000
VERSION     DADJYG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.018"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..1578
                     /locus_tag="JZ382_000693"
     CDS             37..1578
                     /locus_tag="JZ382_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426898.1"
                     /translation="MFFNIVFTQKERRKLAIFHLLEEQHELTIKEISKKLDLSSSVVK
                     NILNEMENEFDDFCFSGFKLLSKNKINQNLPTDLNYDYYYSYLIQQSLPYRAIKSSLF
                     YPEKNLMEFCQENFISRASAMRHLQSLADYVQEFDVIFNRSQLSFKGDERLIRLILFN
                     ILWAASRGNEDYAFYVSKSELLKKLAELTKHTSLNWSFVGKKEVCLFAGITTQRIAQG
                     HYVEDDPRYSEAVFSNDHRIKCLFGEHFSIPSSHQDSEFRFAQFMVFYAPTFQYENPT
                     LNEALDIFDKKGQLLSSLLKELDNYWQTEIFPDDRVFQHNQIVHLHLFNILFCYYLFP
                     KRVPTLFFLMNYFHKKKSSSYYYLKEHFTFFLNKMGRRHNYQWLSICCNDLAEIFAWL
                     TLQCCEDNENSTKLQVSLVMETNYFFTQDIKNFLKDLSFIELKELSYEEIQPIDFLIV
                     SSVHLMPKETDRSYYVVNFLEGKTDYVKLYLELKKAHENKKFHLLLKKTERKKLPSIK
                     PQLFV"
     gene            complement(1938..2294)
                     /locus_tag="JZ382_000694"
     CDS             complement(1938..2294)
                     /locus_tag="JZ382_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAY2426899.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHIGVKQELERAGIQFK
                     RPNKDIVVVEQKNTMD"
     gene            2515..3267
                     /locus_tag="JZ382_000695"
     CDS             2515..3267
                     /locus_tag="JZ382_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY2426900.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRMLAFKLEDIQEILDNPHFDHQKALIEHQQMLLEKRAQIDILLATVQ
                     QTLDRYEGGRKMSDKEKFEGFKQQKLKENEETYGQEIRQKYGEEKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLADLTLYLQAPTDENLADRIFQAHKKWLSYSWSEYQPEAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(3383..4306)
                     /locus_tag="JZ382_000696"
     CDS             complement(3383..4306)
                     /locus_tag="JZ382_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY2426901.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNAKARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEEAFDYDYKVTLLPTGYQKE
                     KERSYLEEFAAKAFDGLIITSRANTLEDLLDYQKYGPLVFCEEIKEKQASCVFIDREQ
                     SITEGLSYLKQQGVKRLGITLGRSGRLSYNSKITLQLCRRLFPFFDESLVFWDCIDAE
                     KGKQAGIFFKDHRVDGVFTNSDMVAAGILQSYLQDKVPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(4321..4593)
                     /locus_tag="JZ382_000697"
     CDS             complement(4321..4593)
                     /locus_tag="JZ382_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426902.1"
                     /translation="MNTYVEITDGTTNKYFYAFVEFKGSILTLYSFQGLNKNIVKEIP
                     MNEIETLTKDIYWGGQRISFSHDGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(4676..6082)
                     /locus_tag="JZ382_000698"
     CDS             complement(4676..6082)
                     /locus_tag="JZ382_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426903.1"
                     /translation="MNGLMSWMEKYILPVATKIGSEKHLVALRDAFIGMMPATMAGAI
                     AVLLNAFMRDFPNTYLGEGNAITTFFTPVIGVNGLIWNGTLAIMAVVFALSLGANLAK
                     AYEVDPVSGSIVSLVAFIIGLPDAATAVLTLPEKLSQDAADLITSAGGVIAGTPDGGQ
                     TLSVGAWGYFNFGKYMGGSGLFTAMIFGFISVIIFAILIKKKIIIRMPDSVPSAVARA
                     FAAIIPALVSLYVVGVINYIFRQTAGEPLIDWISEAIQSPLMNLSQGYGAVFIIVLLV
                     HVLWFFGLHGTNIMSPVLQTLYGTAMVENTNAFQNNEPIPFKWVAGSFEAFVWPGGAG
                     VTLVLIVMILFLSKRADYKTVGKLGIAPGIFNINEPVMFGLPVVLNPLLMIPFILAPL
                     ATATIAYFATMAGLVRPVVVNVIWVMPTIISGFLATAGDWRAIVLTIVNLIVAALIWA
                     PFILAANRLDPNLGEPEE"
     gene            complement(6414..8039)
                     /gene="groL"
                     /locus_tag="JZ382_000699"
     CDS             complement(6414..8039)
                     /gene="groL"
                     /locus_tag="JZ382_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY2426904.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(8091..8375)
                     /locus_tag="JZ382_000700"
     CDS             complement(8091..8375)
                     /locus_tag="JZ382_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY2426905.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            8599..9261
                     /locus_tag="JZ382_000701"
     CDS             8599..9261
                     /locus_tag="JZ382_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAY2426906.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVILAIFYFKQREPLAIESAVKKASVPKIVIYGLVGIFVALILQSIAVMLESILF
                     GEATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVV
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(9338..10636)
                     /locus_tag="JZ382_000702"
     CDS             complement(9338..10636)
                     /locus_tag="JZ382_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426907.1"
                     /translation="MKEWLDRAFLPMVTSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIARWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEENIDRLPVGM
                     TALSAYLLLIQPLNVQVEIDNIWTGGEGILGAVATGLLIGWGYARLARFAKGWRQNGK
                     IPENVWQSFTSLIPILAIVTFTLIGSAVFQQLFGMTVIQGIWTYVQLPIQSLIDTLFG
                     VLLLGFFVPFLWLFGLHGSAMVNGLVSPILQANSLANAEILASGKELTVANGGHIVTQ
                     QFLDQFMTVTGAGLTLGAVFFMTFFAKSRKYRELGKLSLLPAFFNINESIIFSTPIVM
                     NPMMAAPFIFAPILSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQ
                     GIVLAISFFIYFPFLKKIDSANFKKERQTKNDGTAEKIID"
     gene            10806..11435
                     /locus_tag="JZ382_000703"
     CDS             10806..11435
                     /locus_tag="JZ382_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY2426908.1"
                     /translation="MKIAIIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTATDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEILEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLQWTYISPASLFDPHGKR
                     TGTYLLSDDYLKRNAAGDSTISMADYAIALVDEILDKPHEKQHISVVSR"
     gene            complement(11536..12345)
                     /locus_tag="JZ382_000704"
     CDS             complement(11536..12345)
                     /locus_tag="JZ382_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAY2426909.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMKPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENHSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMETVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(12400..13269)
                     /locus_tag="JZ382_000705"
     CDS             complement(12400..13269)
                     /locus_tag="JZ382_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426910.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSDQTESI
                     QKRLASDDITYTGKISSESKQGYYLSAEQTNLSTSLANYRKAGKSGLLESGVFLDGDV
                     LTKSLSSSEAEKNVIDPDKIRSSLEDFLANKDNILFGNEYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(13270..14586)
                     /locus_tag="JZ382_000706"
     CDS             complement(13270..14586)
                     /locus_tag="JZ382_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426911.1"
                     /translation="MRISEKVVRVGLILMVALSLYFSYAIWLSPAGKSSISVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     ESQFEQVKNLSSGFELAYNAPFLLREYKEAFDLDVDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWEDINKELTANDTKWTEMSTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLEAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(14583..16418)
                     /gene="walK"
                     /locus_tag="JZ382_000707"
     CDS             complement(14583..16418)
                     /gene="walK"
                     /locus_tag="JZ382_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAY2426912.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDVKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(16423..17127)
                     /locus_tag="JZ382_000708"
     CDS             complement(16423..17127)
                     /locus_tag="JZ382_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426913.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEATPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(17316..18176)
                     /locus_tag="JZ382_000709"
     CDS             complement(17316..18176)
                     /locus_tag="JZ382_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAY2426914.1"
                     /translation="MKNDKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESIAEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAEQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWNQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASILGTALKLNPIISCNDEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(18166..18732)
                     /locus_tag="JZ382_000710"
     CDS             complement(18166..18732)
                     /locus_tag="JZ382_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAY2426915.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPKKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLESEYLKEEKTNEK"
     gene            19113..19185
                     /locus_tag="JZ382_000711"
     tRNA            19113..19185
                     /locus_tag="JZ382_000711"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19146..19148,aa:Lys,seq:ctt)
     gene            complement(19358..21085)
                     /locus_tag="JZ382_000712"
     CDS             complement(19358..21085)
                     /locus_tag="JZ382_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2426916.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMTGI
                     TNLLLVIPAPLFILLLTIAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKVAGAATQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIKK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(21078..22271)
                     /locus_tag="JZ382_000713"
     CDS             complement(21078..22271)
                     /locus_tag="JZ382_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY2426917.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGIPKEERQEKAEKALENSSLLSFKDQY
                     PNQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(22458..23102)
                     /locus_tag="JZ382_000714"
     CDS             complement(22458..23102)
                     /locus_tag="JZ382_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2426918.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLINQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(23196..23330)
                     /locus_tag="JZ382_000715"
     CDS             complement(23196..23330)
                     /locus_tag="JZ382_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAY2426919.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(23332..25464)
                     /gene="feoB"
                     /locus_tag="JZ382_000716"
     CDS             complement(23332..25464)
                     /gene="feoB"
                     /locus_tag="JZ382_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAY2426920.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVHEQPELIINIIDATNLERNLYLTTQLLD
                     LGIPMIIGLNMMDIIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRLSIPVPCTYDPRLEAALHEIEDVLGEKSSKKRWYAMKYFERDQKVNEDDEITNSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLSVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAEWMQ
                     ELILNGIIAGVGAVLGFLPQLIVLFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMATRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGETAPFIMELPAYHFPQWSTILRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVPEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVASGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(25461..25934)
                     /locus_tag="JZ382_000717"
     CDS             complement(25461..25934)
                     /locus_tag="JZ382_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAY2426921.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGVVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILSQIFVNKKIASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELVLVQREENE"
BASE COUNT         7544 a         5612 c         4507 g         8415 t
ORIGIN      
        1 ttaattaaag aaataagcaa tcaaaaagga ggtcctatgt ttttcaatat tgtgtttacc
       61 cagaaagaac gccgcaagtt agcaattttt catctattgg aggagcaaca tgaattaact
      121 atcaaagaaa tcagtaaaaa acttgattta tcttctagtg tcgtaaaaaa tatattgaat
      181 gaaatggaga atgaatttga tgacttttgc ttttctggat tcaaattatt gtcaaaaaac
      241 aagatcaacc agaacctgcc aactgattta aactatgatt attattattc ctatcttatc
      301 cagcagagcc tcccttatcg cgcaatcaaa tcttctcttt tctatccaga gaaaaatctg
      361 atggaattct gccaagaaaa tttcattagt cgtgcttctg ctatgcggca tctgcagtca
      421 ttagccgatt atgtacagga attcgatgtc atttttaatc gcagtcaatt atctttcaaa
      481 ggagacgaac gcttgattcg cttgatatta ttcaatattc tttgggcagc ttctcgtgga
      541 aatgaagatt atgcttttta cgtctcaaaa tctgagttat taaaaaaatt ggctgaactt
      601 actaagcata catctttaaa ttggagtttc gtaggaaaaa aagaagtttg tctttttgct
      661 gggattacca ctcagcggat tgcacaaggt cactatgtag aggatgaccc tcgctatagc
      721 gaagcagttt ttagtaatga tcatcgaata aaatgccttt ttggtgaaca tttttctatt
      781 ccctcctctc atcaagatag tgaatttcgt tttgcacaat ttatggtgtt ttatgcaccg
      841 acctttcaat atgagaatcc tactcttaat gaagctttag acatattcga caaaaaagga
      901 cagttgcttt cttcattatt gaaagaactt gacaattatt ggcaaactga gatttttcct
      961 gatgaccgtg tttttcaaca caatcaaatt gttcaccttc atttatttaa tattcttttt
     1021 tgttattatc tttttccaaa gcgtgttccg acactcttct ttctaatgaa ttattttcat
     1081 aaaaagaagt catcaagtta ttactattta aaagagcatt ttaccttctt cttgaataag
     1141 atggggcggc gacataatta tcaatggctt tctatttgct gcaacgattt agctgaaata
     1201 tttgcatggc tgactttgca atgttgcgag gacaatgaaa attcaactaa gttacaagtt
     1261 tctttagtga tggaaacgaa ttattttttt acccaagata tcaagaattt tttaaaagac
     1321 ttgtccttta tcgaactgaa ggaattatcc tatgaagaaa tacaacccat tgattttctg
     1381 attgtctctt ctgtacatct tatgcccaaa gaaacggata gaagttatta tgtagtgaat
     1441 tttttggaag gaaagacaga ttatgtcaag ttgtaccttg aattgaaaaa agctcatgaa
     1501 aataagaagt ttcacttgct tttaaaaaaa actgaaagaa aaaagctgcc gtcaattaaa
     1561 ccacaattgt tcgtataatt ggattgcaaa aaagggctgc gacaaaagtc tgtcacagtc
     1621 ccttttccca aataaacggt gttctcctgc caactcctcg gtattagctc aacaaacggc
     1681 acaatatgag gaactatttt tccgtttgtt tctctaatac acaggtcaaa acgctgtttt
     1741 cacaacctct tccttaaacg gtgctcaaaa gcttgcatct gtggtaataa gcccaaccaa
     1801 acaaaaaata tgacgaacta tttttgtttg gctgttctta ttacttgatg cagaacagct
     1861 ttttcgcaac ctctttttta ctgtatacat cataaagatg gagcgattta gcataaagat
     1921 gattagtcgt taggacatca atccatcgta tttttttgtt caacaactac aatatcttta
     1981 tttggacgtt tgaattgtat tccggcacgt tccagctcct gtttcactcc tatatggact
     2041 ttgacatctt cccgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt
     2101 gtgatttttt tcttcgtatt gatcatcaca acttgctttt tgtcatatgt gtccactagc
     2161 agcaactgtg tccgatctaa cgaaaataat ttgtaataat taatggccgt attcccaaaa
     2221 aagaattcta ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt
     2281 tcgattagct tcatctcatt cctcttttct atatagtcga tttagattca atcccatata
     2341 ttccccgttg ttcccaacat taaggaatcg gttacaattg aacctttaaa aacaaaagga
     2401 tttacctttt atcacttaat agtataacat aattttatga gaaaaaaata cacttttttc
     2461 ttgaccttct cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggaa
     2521 tacacgataa agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat
     2581 gatgagattg gactgctaaa gccagctagg atcaattcct caggttatcg gatttatggc
     2641 aaaaaagaag tcgatcgtct gcaacatatc ttattttatc gaatgttggc attcaagttg
     2701 gaggatattc aagaaatctt agataatccg cattttgacc accagaaagc tttaatcgaa
     2761 catcagcaga tgcttctaga aaaacgggca caaatcgaca tcctcttggc aactgtccaa
     2821 caaacacttg atagatacga aggaggaaga aaaatgagtg ataaagaaaa atttgaaggg
     2881 ttcaaacaac aaaaattgaa agaaaatgaa gaaacttatg gacaagagat tcgacaaaaa
     2941 tacggggaag aaaaggttac agcatccaac gaaaaatgga tgaatatgga taaagaaacg
     3001 tatgaagaaa tgcaaactgt tgaaaagcag atgttagctg atctcactct ttatttacaa
     3061 gctcctacag atgaaaactt agcagatcgc atctttcaag ctcataagaa gtggttatct
     3121 tacagctggt ctgaatatca gccggaagcg cataaaggat tagggatgat gtacgaagcc
     3181 gatgaacgat tcactgcata ttatgacgaa cgaagtggtc atggtgcagc aaaagcatta
     3241 aatgaaatca tccaaaaaaa ggcataacag tttacatgat ccgacaaaaa cgaccgaaca
     3301 acaaaacatc agacaccaaa gttagatttt ttatactaac atttggtgtc tgattcattt
     3361 tttacaagtg ttttgccaaa attcagcgct gaataaacgt gtaaggtatt ttcactttat
     3421 cctttttttc tgtaagaaac aattgaaaag ccatttctcc gcaagcttcc agatggtgat
     3481 caatcgttgg aaagccaaga agctcgctga tcagcaaatt atcgcgtcca atcaccactg
     3541 gtaccttatc ttgcagatag ctttgcaaga tgcctgctgc gaccatatcc gaatttgtaa
     3601 agaccccatc aactctatgg tctttaaaaa agatacctgc ttgttttcct ttctctgcat
     3661 ctatgcaatc ccaaaaaaca agtgactcat caaaaaacgg aaaaagtcga cgacatagtt
     3721 gcaaagtaat ctttgaatta taactcagcc tgccacttcg ccccaatgtg atgcccaatc
     3781 gttttacccc ttgctgcttc aaataagaaa gaccttctgt aatcgattgt tctcgatcaa
     3841 taaagacgca acttgcctgt ttttccttga tttcttcaca aaaaactaga ggaccatatt
     3901 tttgataatc tagcaaatct tctagtgtat tggcacgaga tgtgatgatc aatccatcaa
     3961 acgctttagc tgcaaattct tcaagatagc ttcgctcttt ttctttctga taacccgtcg
     4021 gtaaaagcgt cactttgtaa tcatagtcaa acgcctcttc aatgatccca ctgatcagct
     4081 gatcaaagta cggatggttg gtataaggca agattactcc gatacttttc gtttttccat
     4141 aactcaactg tctggctttt gcattaggga tataatcgac ttctttcatg accgcaagta
     4201 tttctttcct tttttcctca tctacatatg gatgtccatt gatcacacgt gaaatagtag
     4261 aaactgaata acctgtgagc tttgcgatat ctcgaatatt tgccactgct gcttcgccac
     4321 ctatacaaaa agattttctt gtaaatagtc taccacagct ggaccgcatt caaaaaactg
     4381 atacattttt ccatcatgag aaaaactgat ccgctgtcca ccccaatata tatcttttgt
     4441 caacgtttcg atttcattca ttggtatttc ttttactata ttcttattca gcccttgaaa
     4501 actgtacaac gttaatatgg atcccttaaa ctcgacaaac gcataaaaat atttgttcgt
     4561 tgtgccatct gttatctcta cataggtatt cattgcaagc cctccttttg atactttgag
     4621 tatagtgtat gaaacgcgtc tcatatcaag gagtttgttt tgcaatagaa aaattctatt
     4681 cctctggttc acctaaattt ggatctaagc ggtttgctgc taaaataaat ggcgcccaga
     4741 ttaaagcagc tactattaaa ttaacgattg ttaaaacgat ggctcgccaa tctcccgcag
     4801 tagctaaaaa tccactgata atcgttggca tgacccaaat cacatttact acaaccggac
     4861 gaacaagccc ggccatcgtg gcaaaatagg cgatcgttgc agtcgcaaga ggtgccaaga
     4921 tgaacggaat catcaacagc ggattcaaca caacaggaag accaaacatc actggttcat
     4981 tgatattgaa gattcctggt gcaatcccta actttcctac cgttttataa tctgcccgtt
     5041 tagataaaaa taaaatcata actatcaaca ccagcgtgac tccagctcct cccggccaga
     5101 caaaagcttc aaaagaacct gccacccatt tgaaagggat gggctcatta ttttgaaagg
     5161 cattcgtgtt ttcgaccatc gcggtaccat ataaggtctg caaaacaggt gacatgatat
     5221 ttgtgccgtg aagaccaaaa aaccaaagaa catgcacaag aagaacaatg ataaagaccg
     5281 ctccataacc ttgtgacaag ttcatcaaag gagattgtat ggcttcagaa atccagtcaa
     5341 tcagcggctc accagctgtt tgtctaaaga tataattaat cacaccaacg acgtataaag
     5401 aaactaatgc aggaataatc gccgcaaaag ctcttgctac agcagatggc acagaatccg
     5461 gcatccgtat gatgatcttt ttcttgatca gaatcgcaaa aataatgaca gaaataaatc
     5521 caaagatcat agcagtgaac aaaccagacc cgcccatata tttaccaaaa ttaaaatagc
     5581 cccatgcgcc tactgataac gtttgtcctc cgtctggcgt tccagcaatc acaccacctg
     5641 cagaagtaat caaatctgct gcgtcttgcg aaagcttctc gggcaatgtc aacaccgctg
     5701 ttgctgcatc gggtaagcca ataataaaag caaccagaga aacaatactt cctgagacag
     5761 gatcgacctc ataggctttc gctagattcg ctcctagtga taaggcaaag accactgcca
     5821 taatagctaa agtaccattc cagatcaatc catttacgcc aatgactggg gtaaaaaatg
     5881 tcgtgatcgc attcccttca cctagatacg tattcggaaa atcccgcata aatgcgttca
     5941 gtaaaacagc aattgctcct gccatcgtag ctggcatcat accaataaaa gcatccctca
     6001 atgcaaccaa gtgtttctcc gacccgattt tcgttgcaac tggcaaaata tacttctcca
     6061 tccaactcat taatccattc attttttctc ctcctgtttt tattgttacc gaagccccgt
     6121 tttgaatacg catacattat aatgatatta taaatatagc atagttattc ctatctggct
     6181 atctgttttt agcagataaa ataaataact atgcaaattc aaaaaatatg cataatcctt
     6241 tataaatagt atagtttcca actactcgca aaagcaaatg atacccttta aataacgatt
     6301 attatgaatg gatatacaaa aaaagtagaa aaggaaatat tttgcttcct tttctacttt
     6361 tttatctatc ttcctcaaat aatgggctac gctagataag caaggtgctt ttcttacatc
     6421 ataccgccca tcattgatgg atccattgca ggagctgctg gtgctgctgg ttctggttta
     6481 tctgcaacga ctgcttctgt tgttaataac aaggcagaaa cggaagcggc attttgcaag
     6541 gcagaacgtg ttactttagt tgggtctacg atacctgctt caaccatgtt tacccattct
     6601 ccagtagcag cgttgaatcc agtacctagt tctacgttct tcaatttatc aacaatcact
     6661 gatccttcat aaccagcgtt ttcagcgatt tgacgaattg gttcttccaa ggcacgaaca
     6721 acaatcttga tacctgttgc cacgtctcct tctgcttcta cagcagaaac tttgctgata
     6781 acattcacta gagctgtacc accgcctgag accattcctt cttctactgc ggcacgtgta
     6841 gcattcaatg catcttcaat acgtaatttc aattctttta attctgtttc agtagctgca
     6901 ccgactttaa caacagctac accgcctgct aatttagcta agcgttcttg taatttttcg
     6961 cgatcaaaat cagaagttgt ttcagcaatt tggtttttga ttaattgcac acgcgcttcg
     7021 atggcttctt tttctcctga accttctacg atcgttgtat tgtctttatc tacaacaact
     7081 ttagacgcat ttcctaagtt ttcgattgtt gcatctttta attctagacc tagatcgtca
     7141 gtaattactg taccacctgt caaaatagcg atatcttcaa gcattgcttt acgacgatca
     7201 ccgaagccag gagcttttac agctactaca ttgaatgttc cgcggatctt gttcaaaaca
     7261 agcgttggta atgcttcgcc atctacatcg tcagcaatga tcaataatgg acgtgattgt
     7321 tgcaagattt gttctaacaa tggcagaatg tcttggatgt tagagatttt tttgtctgta
     7381 atcaagatat atgggttttc tagaacagct tccattttgt cgttatctgt taccatatat
     7441 tgtgataagt aaccacgatc aaattgcata ccttctacaa catctagttc tgtttcgatt
     7501 ccttttgatt cttcaattgt gatgacaccg tcgtttccaa ctttttccat tgcatcagca
     7561 atcaaatgtc caactttatc agaacctgaa gaaacagctg ctacttgtgc aatggcttct
     7621 tttgaatcaa caacagttga gatattatgc aactcttcca ctgcggcttt tgttgccaat
     7681 tcgatcccgc ggcgaatgcc tagaggatta gcacctgcag ttacattttt taaaccttcg
     7741 cgaacgattg cttgtgtcaa aacagttgct gtcgttgttc cgtcaccggc aatatcattt
     7801 gtttttgatg ctacttctga tacaagtttt gcacccatgt tttcaaaatg atcttctaac
     7861 tcgatttctt ttgcgatcgt cacaccatca tttgtgatca atggagagcc gtaagatttt
     7921 tctagtacga catttcggcc tttaggtcct aatgttactt tcactgtatc tgctaattta
     7981 tctaccccgc gtaacatagc tgcgcgtgcg tcttctgcaa attttaattc ttttgccatg
     8041 attgattcac ctcatcgatt atttttgatt attttttcta tcttcaaaat ttactcaaca
     8101 atcgccatga tatcttttcc tgcgacgatc agatattctt ttccttcgta ttttacttct
     8161 gtaccggcgt atttttcgaa cataacttgg tcgcctgctt tcacagcggc aggaactttt
     8221 tcaccgtttt ccaacaaacg gccctcaccg actgcaacga ctgtcccagt ttggggtttc
     8281 tctttagctg cagaagctaa aacgattcct ccgacagttt tttcttcttc ttttgcgact
     8341 tcgataatga cgcgatcacc taatggtttt aacacgataa atccctccga taactctttt
     8401 tttaatccgt tagcactctt aaccttcgag tgctaaccta catttttaat aatactcatt
     8461 ctccctcttt tttgcaagct tttaaatcaa agaaaagaaa ttttattctg acagttgttt
     8521 attgaaatta tgatataatt aaaatcaatc gataaaagct tgccttgcat gcttgttatc
     8581 ccttaaagga ggaattacat gtctttaaaa aaatatagtt tcctcagcat tttttgctat
     8641 ggtctaattt ttatctcacc cttgatctta gcttcgatag gacttgtaaa atctacgtct
     8701 gaattgatca cagcaactgc agtcatgtat atactgggcg cagttatcct agctattttt
     8761 tattttaagc aacgtgaacc gttagcaatc gaatcagcag taaaaaaagc ttctgttcca
     8821 aagatcgtta tttatggatt agttggaatc tttgtcgcat tgattctgca aagcatcgct
     8881 gtcatgttgg aatctatatt gtttggcgaa gcaacccctt ctgagaatac acaaaatatc
     8941 atccagatga tcatggaagc acctgctttt attttggcga caactgttgc cggaccaatc
     9001 atggaagaat tcgtattccg ccgcagcata ctggggatca tcagtcggta ttcaaatttt
     9061 tgggttgggg cagtggtcag ctctttgctg tttgcgtttg ctcataatga cggtcatttg
     9121 ctaatttatt ttttcttagg tttcttcttc agcctagagt acaaagcgac tggtcggatc
     9181 tggacatcga tgatcacaca cgtaggaatg aacacattag ttgttcttgt tcaattagct
     9241 gtccaaaaag gactggtata aacatgatta aaaatactcc tgtcatccta gattggtgat
     9301 ttttccaatc taaagcgaca ggagtatttt tgttttttta gtcgataatc ttttctgctg
     9361 ttccatcatt ttttgtctgt ctttcctttt tgaagtttgc agaatctatt tttttcaaaa
     9421 atgggaaata aatgaaaaaa ctaattgcca gcacgatacc ttgtaaaatc gctgcaggta
     9481 ctccgcctac taaaaaaccc gagaggatcg gcggtgttgt ccaaggaact tggaccgctg
     9541 taaataaagg aacgaatcca aaatataaag cggaataggt aatcaatcct gagaggatcg
     9601 gcgcgaaaat aaaaggagca gccatcatcg ggttcatcac gatcggtgtg gaaaaaataa
     9661 tcgattcatt gatattgaaa aaggctggta aaagtgaaag ttttcctaat tctcggtatt
     9721 tcctggattt cgcaaaaaac gtcataaaaa atacagcccc taacgtcaaa cctgctccgg
     9781 taacagtcat gaattgatca agaaattgct gagtaacgat atgcccgcca tttgccacag
     9841 tcaattcttt tccagaggct aagatttctg catttgctaa tgagtttgct tgtaagatcg
     9901 gactaaccaa cccattgacc atcgcagatc catgaagacc aaatagccat aaaaacggga
     9961 caaaaaaacc taataaaagc acaccgaata acgtgtctat caacgattgg atcggaagtt
    10021 ggacgtacgt ccatattcct tgaatgacag tcatcccgaa caactgttga aaaaccgctg
    10081 aacctataag cgtaaaagtg actatagcaa gtattggaat caatgaagta aaagactgcc
    10141 aaacattttc aggaatcttc ccattttgcc tccacccttt cgcaaatctt gctaaacgtg
    10201 catagcccca tccaatgagg agcccagtag caactgctcc tagtatccct tcaccgcctg
    10261 tccaaatatt atctatctct acctgaacat taagtggctg aataagtagt aggtaagcag
    10321 acaaggctgt catcccgact ggcaagcgat cgatgttttc ttcttttgca taagcatacg
    10381 caatagcaaa aacgatccaa aaggctatca cgtcaaatgt agcctggtat acttgtgtaa
    10441 aatacataga aaatcctgtt ttatctatcc accgagcgat gtaatctaca ggaaaatgag
    10501 caaataagaa aaacagtgag ccaactataa ttatcgggat cgtataagtc attccattac
    10561 gtaaagcagt taagggtcta agattcacaa cagatgttac cattggtaaa aaggcacgat
    10621 ctaaccactc tttcatttct tatctccttc atcttctatt tgatcaacta gttttcttaa
    10681 tttaactata cccaatgatc acgaaccatg aaaggaatgc gcactaagct tttcaaaaaa
    10741 cggacgcttt gacgaatatc cagtaacctc ttacaatgga caggaataaa aaagggggtt
    10801 tttttatgaa aatcgcaatc attggcgcta ctggtcatgc aggttcattt attttagacg
    10861 aagcattatc cagagattta gatgtgacgg caattgtgcg tcatccagaa aaattaccat
    10921 cagatgttcc gtttatacaa aaggatttat ttgagttgac agctactgac cttgcatctt
    10981 ttgatgttgt cgttgatgct ttcaacgctc ctaaaggaaa agaagaaatg catcaaactt
    11041 ctttgtccca tttgattgag atacttgaag gaaccgatac acgcttaatt gtcgttgggg
    11101 gtgcctcttc gttattctta gatacagaaa agacgactcg tatgattgac caagtaccta
    11161 aaaatgcacc cttctatcca acagctttca atatgtacga aggattgtta aaattgaaac
    11221 aatctaaaaa tctacagtgg acttatatca gtccagcttc tttatttgat ccacacggca
    11281 agcgtacagg cacttattta ttaagtgatg attatttaaa aagaaacgct gctggcgaca
    11341 gtacgatcag catggcagat tacgcgattg cattagtaga tgagatactg gataaaccac
    11401 atgaaaaaca gcacatcagt gtagtcagcc gataatacga ttgttttgtc aatgatctga
    11461 aaaaataaaa acagaagata aaaccaacac tgattgattt tatcttctgt ttttattttt
    11521 tattgactac actgtttaaa tcgaaaatag ctcagttgct tcatctggat ctgtatcata
    11581 gatttcaaaa tcatgtccaa caccaaaatc tttttctttc aaaacagttt ccatcgttaa
    11641 atgagcaagt tctttcatat tgttttcctt gctaagatga ccaagataga ttcgtttggt
    11701 ccgattaccg atcacatcag tcatgacaag tgcaccatct tcgttggaaa gatgccctct
    11761 atctcctaaa atacgctgtt ttagattcca cggataaggt cccatgcgta acatctcaag
    11821 atcatggtta ctttccatca aataaccatc ggcattttca atgacacctc gcatgtaatc
    11881 actgcagtat ccggtatccg tcaacatgac gaatgaatgg ttttctttat ggaaacgata
    11941 aaattgcgga gctgctgcgt catgggagac gccgaaactc tcaatatcga tatcgccgaa
    12001 tgttcttact tttcccatct caaatagatg tttttgttcc actggtacat tcccaatcag
    12061 tggcttcatc gcttcccaag tctgctgatt ggcaaaaaca tccaaatgat acttacgagc
    12121 taagacacct acaccatgta tatggtctcg atgttcatgc gtcacaagta ttccatctaa
    12181 ttggtccggt gtccgatcaa tttgagccat caaggaagtg atttttttac cgctaagtcc
    12241 tgcatcgacc aaaagccgtt ttttttcact ttcgatataa agtgaattac ccgaacttcc
    12301 acttgctagg atactgattt taaaagctgg tttttcttca gccatttgac tcctacttcc
    12361 cttcttactt tgtaaacttt tttcattata ctcgcttttt tattgatctt ccacttttgg
    12421 tacagtgttg tttgtcacga tcgtattgct gaaagcgttg acgctctcga tttgaaccac
    12481 tttatcactt gtttcaatcg ctacatgcca aacaggaaca tacacatttt tttcccggac
    12541 tttcagaata caggaataag ctagttgacg ccataagatt tttgatcctc ttggtatttt
    12601 gttattgatg tacaatgtat tgattgcttc ctcttctgta tacagttccg tcttttcacg
    12661 cagctgttcg atatcagata aatgcgtctg catgtatttc gtgatactat aatcgttatc
    12721 gtttttttct aaagaaagaa cgatttttgc tgtatcatca ttgaacggaa taccttcata
    12781 ctcttggctt gcttggattt ctagtggttc ttcttctaaa tgagaaaagt ttttcgtata
    12841 cacatactca ttaccgaaca aaatattgtc tttgttagct aaaaaatctt ccaaagaact
    12901 gcggatctta tctggatcaa tcacgttctt ttcagcttct gagctggata aggattttgt
    12961 caaaacatcg ccgtctaaaa acacgccact ttccaacaat ccgcttttac cagccttgcg
    13021 ataatttgct aatgaagtgc taagattggt ctgttccgcg ctaagataat agccttgctt
    13081 gctctcagaa gaaatcttcc ccgtataagt gatatcgtca ctagccagac gtttttggat
    13141 tgattcagtc tgatcagaac gaataacatt gttttcttgg tataccgcat catgataact
    13201 gctgaataaa aacacattca accctaaaaa agcgacgaaa aatatccatt cgattttttt
    13261 aaaatccaat taattcgcct ccaatccagg taatttttcc agaaggtcgt tagctgaata
    13321 ccagttatct ttatacttca cataccattc aggtaagaga tcaacgacgc gattcgtttc
    13381 tttaacattt tgccatgtat agccaacgat tactgaacca atcttgttgc tatctgctcc
    13441 cttagcttcc agctgctgga tgatttcatt cgtacttgca agttctactt cttcatctga
    13501 aggtatcggt acttgaatcg tattcaaact ggtttggata ttcacttgca ccgaaccgtt
    13561 cgtttcaccg ccaacttcca caccaatttg tcctctgctg tccgtcccaa aaaccgggaa
    13621 gccttccacg aagatccggt aatcgatggt attgtcactg cggtcaaaat atctgagatt
    13681 ccctaaagaa ctgcctaatt tgctaatata gggaaaactt tcagaaaata cactagcatt
    13741 ttcttttttc tctggcagct ctcctcgaaa atcaactact tgcgtttccg aatggataga
    13801 catcgtttca ttcccaccgt aaaaaatcaa ttcattgctt tcatcattat tcttcacgct
    13861 atctggtttc tggaaaaaag catttctaaa aagtgtatag ggttgctgcg aaaggatata
    13921 gctatacttt ttcaacttga ttgaatgttt cgtagcatat tgggtgtcga tcgtcgacgg
    13981 ttctgttgac atttctgtcc atttcgtgtc gtttgctgtt aattctttgt tgatatcctc
    14041 ccaattcaat gaaatattcg cttcatatac aaggtggttc gaataattca aaaatcggat
    14101 ttttttgttt ccttgatcga attgtattct agtaaaatac atattttcgc tttcttttgc
    14161 caaattcgag acgtctacat ctaaatcaaa tgcttcctta tattctctta gcaaaaaagg
    14221 cgcattatag gccaactcga aaccagatga cagatttttc acttgttcga attgtgattc
    14281 atctcccgaa acgacttcag aaagcttccc gaatcttgct ttatcgatga tcaattgcaa
    14341 tcggtacacg agattttcac tgttggtttc ttgaatcatt cccgaatggt gccatgtgac
    14401 atgaagcgga agaaaaactt cagaagtttt ccgattgctt tgagactcga tcatttgagt
    14461 gtttgtctca tcgacgctga tcgaactttt ccctgctgga ctcagccaga tggcataaga
    14521 gaaatataaa cttaatgcaa ccatcaaaat aagtcctacg cgaacgactt tttcagaaat
    14581 tctcattccc accattcctc ctcataaggt tcgtatggca gcgagatata aaaagtcgat
    14641 ccttttcctt ccttgctctc tgcccaaata gcgcctcggt gcgccttgat tacttctcta
    14701 gaaatagcta atccaagtcc tgtcccacct tgtgctcttg cacgtgcttt atctactcga
    14761 taaaatcgtt cgaacacacg gttcagatct tttttaggaa tccccaatcc ttggtcggta
    14821 atacttaaaa tcacgttatt atgcgtttct aataagcgac aagtgattgt tcccccatct
    14881 ggtgaatact tgatcgcgtt attcatgata ttatcgacta cttggatgat tttatctgta
    14941 tctacttcga cccaaagatc tctttgcgtg aattcacgac ggatcttata atttttttct
    15001 tggtttccca ccatcatatc aaaacggtca agaacaaaat tcactaactc gttaaaattg
    15061 acatactcta attgaagctg ggtatttcct gtatccatgc gagatagatt caggagatca
    15121 ttgatcattc gaatcatccg atctgtttca tccaatgtta ctttcaaaaa atttggcgct
    15181 acttcttcat ctttccatgc accttcgctc aatgcttcga tataactgcg catgcttgtc
    15241 agtggtgtac gaagctcatg ggaaacatta gaaacgaatt cacggcgttc ccgttctgtt
    15301 ttttcttgtt ctgtcacatc atgcaagaca gctactaatc cgctgataaa acctgattca
    15361 cgacggatca tggaaaagtc tactcgtaag atcatctgat cgctctccaa tgtactggtt
    15421 gaacggtcaa gtaccatttc gtttggatct tctaataatt tacgcaaggt atattcttct
    15481 tcgatatcca aaagtgtcaa aatcgattgt ccaattgctt cctcattttt gacatctagc
    15541 aaggaaacag ccatatcatt aattgtaatg actttccccc tgcgatctgt cgcgatgacg
    15601 ccgtctgtca tatgagacag cacactatcc aaccgattgc gttcagcctc cattgtatct
    15661 tgagcttctt cgatacgttc agctaactga ttgaaggtat cagccaactg ccctagttca
    15721 tcttgtccat gcactttgac tttccggctg tagtctcctc tagcgattcg aatggcttgt
    15781 tctctcattt caccgattgg ctgagtaatc gaacgagcca ctaaaacgga gacgatcatt
    15841 gaaataaacg cagcaatcag tgaggcagta aagaaaatga ctgctgtatt attgatttcc
    15901 gaatattttt gttcgatatt acttttgacg taaagagccc cgataacggc atcgccggtc
    15961 ggggacagga tcggttgaac attgatattc acccgtttat cgtcgttatc taaagcaaca
    16021 taccgtttag ttgtgaaatc attgatatcg acataatcat ttttctttcc cacagcgctt
    16081 tgttcattga catcggttgt tcctcggatg atcccttttt cgtcaacgac ccgtatttcg
    16141 atgatatcgc tagtcgcact gttttcaata aatctcttga tattcgcatt tacctcttca
    16201 tcatctgcat tatcttttcg attcaattcc ggactgattg ttgttgctaa tgattccacc
    16261 gtttgattca tatctttaat aaatgtattg atcgtggaac gttctaaacc acgtataaag
    16321 taggcaccaa taatttcgat cgagatcaat aaaatcagaa taaaggtaat agcgatctta
    16381 aaattgactg atcgaaaaaa gcggactttt cttttcatcc aattactcct gttctgggtt
    16441 acgcaaatag taaccgacac cacgacgtgt cactaaataa tttggatggc ttggattatc
    16501 ttcgatcttt tcacgtaaac gacgtacagt tacatccact gttcgtacat ctccaaaata
    16561 atcataaccc caaaccgttt gtaataaatg ttctcttgtc atcacttgtc caaggtgctt
    16621 cgccaagtaa aagagtagtt cgaattcgcg atgtgttaac tcgattgttt ctcctcgttt
    16681 cgtcaccatg taagcttctg gatggatcgt caaatcacca atcgtcaatt cagcaggtgt
    16741 tgcctcttct ggttccttcg cagccgtggc accacgacga agatttgctt tgacacgtgc
    16801 aactaattct cggttggaaa atggcttggt cacgtaatca tcggcgccta actctaagcc
    16861 taggacttta tcaatttcag aatctttggc tgttaccata atgatcggca tatcataagt
    16921 tttgcgtacc tcacgagcca cttctaagcc gtccatttta ggtagcatca aatcaagtaa
    16981 aatcaaatca ggttctacct cagcaacttt ttccaaggct tcttccccat catatgcagt
    17041 ataaacatca tagccttctt tggctaaatt gaatttgacg atatccgaaa tcggcttctc
    17101 atcatcgaca actaaaactt ttttcataaa gagccacctc ttatatatta ttgtcacacc
    17161 ttttcattat accgtatttc tggttttgtt tcatctttgc ctataaaaca aaagagaaaa
    17221 ttaacatggg agtgcaaaat actttctttt tttgttctaa cttcaataaa aaaaggaaaa
    17281 cactgatttt aacctcactg ttttcctttt ttgatttatc cttctaaaac ttgtacgccg
    17341 acacctaata atcccggacc agtatggact actaatgccg gagaaatttg accaaagtag
    17401 atttggtttg cttgcgggaa ctctcttttg agcctcgctt ccatttcaat cgcttcttct
    17461 ttcgcatcac cgtgggccac ggctaagtta aaaaccttgg cattgccgat tttttctttg
    17521 acgtaagcaa ccgtcttatc caaacttttt ttacgtcctc tagcttttcc tactgtgtaa
    17581 tagattcctt catcattaca ggagatgatt gggttaagct ttaaagcggt tcctaaaata
    17641 gaagcaacca aaccaatccg tccacctttt tggagatatt ccaatgtcgc aacattaaag
    17701 aaaacttttg ttctttcgac taattcattc aaattactga tcagctgatt ccactcgaca
    17761 ccttcttcta accatttcgc tgcttgtatt gcagaaaaac ccgcaccaat accgatattt
    17821 ttcgtatcta gtacatacgc ttccaattct tcttgctgtt ctgcaatcaa acgaactacg
    17881 ttatacgtcc cgctcaatcc gctggaaatc gttaccgcaa gtactttttc ataaccatct
    17941 tttttgattt gctcaaagat ttctgcgatc gactcgccgt caggcaaaga tgtacctgga
    18001 atttcctctg gcaaacgttg atagatttct tcaggtgtaa tatctatttt atctgtatat
    18061 acctgatctt tataaataat tttcaaagga atcatgtaca tctcatattt ttttatcaat
    18121 tcttctggca cgtccgtacc agaatctact aaaatcgcga ttttatcatt tttcatttgt
    18181 tttctcctct tttaagtatt cggattctag aacaatcgtt ttttccgcaa gaagtttcat
    18241 tgcaaacgat aaagttgctg ctcttacagc aatatattct acaggggtcg cttggtcgaa
    18301 ttttttggga tgggatttgc cctgtgctaa ttggctgacc atccatacag ctgcttcttg
    18361 ttcatcacag aatagattat aagcttcacg aatccccact gtttttcctt ggaacaatat
    18421 tccttctttg atttcaggaa tagtcaatac ttgtttcaat gtagtgattg caagtaaaaa
    18481 tgctacatgt tctttgttgt atcttttttt aactggtgct ggtatcaatc ccagtttaac
    18541 gtagttgttt accatagagg aggtcagcaa tggatgcttt tctccttgta tcacaggtga
    18601 aagataacga tcaactaacg ttctgacttg atccatataa agttctaggt cggggagctc
    18661 ttcccagtga ggcagacgaa atgtttccag ttcttttccc cactccatta attgctgacg
    18721 cattttttcc atcgtaaccg ctcctttttc atttcattaa catgatacca gagttataat
    18781 aaacattcaa tctagttgtt ttaactagat gatttaatta atggaatttt ataaaagcaa
    18841 aaaaacaaga aggccaagcc ttcttgtctt ttatcgttaa gaacaggaga cccgccgggg
    18901 agacggcggg aaaggagtaa aaaatgaaaa aagtgttagg gtttgttctc ttgcaagaac
    18961 tttgtttgtt ggtatgctta tatagtaacg agcaaatgtg aataatctat gaatgatttc
    19021 ctataaggaa gtaaagattt ttctgatttt ctttaaatgt agtcttgcat tttaaaattc
    19081 ttagtgctac tattattaag tactttttgt atgggccgtt agctcagttg gtagagcagc
    19141 tgactcttaa tcagcgggtc gcgggttcga gcccctcacg gcccattggg tgccaaaccc
    19201 acgagaatgg tagtttgtcg gcgtcttcgg acgtttcgaa gatgaactgc gcattctctt
    19261 ttttatagac aaaaaaagag acagacgaat atggagcttc caaaactgga tttttgatcc
    19321 aattttggaa gctccattta ttttgtttcc gcctttttta ttttttccag ctttcgacta
    19381 attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt ccattgttga
    19441 tttccaacat gactttttcc atatcttttt ctgaccaatg gaaattgtct aatactttat
    19501 aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc tgctcttcgc
    19561 ttcccattcc gttttcagga tcttccaagt atttcagatc atatttggca aacatccaat
    19621 gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gattgcttgt cctagtgcaa
    19681 cggtcattgc tcctgaagaa gatgtctcga ctttccagtc tttcaagttg tcatattttt
    19741 tgattgtgtt ttcagcagcc gtaactaccc cagctcctgg ctcaattcct ataattttct
    19801 tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct ggtactgcta
    19861 aaccgacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct ttatattgtg
    19921 catactgtga accgtgagtt ttaggtagcc aagctgaaac catggcatct gactcgccat
    19981 ttgaaacaga tttccacatg attgaattat ctagcggtgt catcgttaca tcatagccca
    20041 tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc cattcgacat
    20101 aagacaagtt gatacgtttt gtttctttgg cagaagaaaa acttcctata ccaatcgcac
    20161 ctaagatcac tactactaca gcaccaatga tcaatccttg acgtttttta cttttttgtg
    20221 ttgcagctcc tgcaactttc ttattgtttg gctgattcaa atgctgcgta aaacggtcaa
    20281 tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg acttgtgctc
    20341 tttgtaaagc ggacaatact ccccgtccta agccgggtgc accgatcata gaggcaataa
    20401 cgaccattga aagtgacagc atcgttgttt gattgatccc tgccatgatc gtacttttag
    20461 ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat gaatcagaag
    20521 cttctaccaa ctctttcggt acttgtcgaa tccctaaatt cgtaaagcga acggttggtg
    20581 gcaaagcaaa gatgactgag gcaaataccc ctggcaccat cccgataccg aaaaatgcaa
    20641 ctgctggaat caaataaacg aatcctggca tcgtctgcat aaagtcaagg atcggcttga
    20701 tgatgctttg agctttactg ctttttgcca tcaagatccc taacggtaca cctatgatga
    20761 ttgagatcac actagataaa agaacaagtg tgacagtatt catcaagtca ttccatagac
    20821 cttggttata aataaaccat agaccaatca acgtgaacaa cgtaagtcct ggacgtttct
    20881 tagagataaa gaaagctgct atcgtcaata acaagataaa caaaggcgct ggaatcacta
    20941 acagtaaatt tgtaatcccc gtcatcactg cctgtccgat cgtttgtaaa aatgaaaata
    21001 ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt gccactggta
    21061 attgatagtt taatagatta ttcatgttca ctcacctcgc ttgtttctgc taatgcttca
    21121 atcacactac ctcggatgac gacacctttt aattttccat cctctacaac tgcaagcgga
    21181 gtcggtgaat cataaataat attgaaaata tcacttacaa ccatgtcttt tccgatcacc
    21241 gtcacatttt catctaagaa gtcgatcaat ggacgttttt gcttcctagc ttctaacgcc
    21301 tgatctgcag taatgattcc tttcaggtgt ctctttcggt caacagccat cagcatgctg
    21361 acttcttcat tacgcatcct tgtcaatgcg acatttggtc catcagattc gatattcgtc
    21421 gtcaatgcag gtaccatgat gttttgagca gtaagcactt tggaacgatc gacttcttcc
    21481 acaaattcac gtacgtaatc atttgctgga ttggtcaata tttcttcacc tgttccgatc
    21541 tgcatgattt cgccgtcttt cattaacgcg attctgtccc cgatccgtaa agcttcattc
    21601 aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaagtc cagtaattcg
    21661 tcttgcattt ctcgtcggat cagcggatca agagccgaga acgcttcgtc catcagcaag
    21721 atctcaggat cgttcgctaa agctctggct aagccgaccc gttgctgcat cccaccagat
    21781 aactgattag gatattggtc tttaaatgaa agcagactag aattttctaa tgctttttct
    21841 gctttttctt gtcgttcttc tttaggtata ccacggactt ctaacccgta ttctgtattt
    21901 tccaagatcg ttcggtgtgg aaacagaccg aagttttgga aaaccatatt gattttatga
    21961 cgacggactt ctcgcaattc ttctttactt aacttcgata catcttctcc atcaatatag
    22021 atactgcccg aagttggttc gatcagacga tttaatagac ggatcaatgt ggatttccca
    22081 ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa attcacgtca
    22141 tacacaccaa ccgtcgcacc ggttttcttt aaaatctccg ttttcggctg gtgatcattc
    22201 atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg ttttacttct
    22261 acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct gaaaaaagtg
    22321 accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat ttgcttgtct
    22381 aaacagaaaa aaacttggtt aaatctttat aaataaaagg tttagccaag ttaatatttt
    22441 tataatattt cacacaatca ttctgcaagc tgatgttccg ggtaaaaaaa gtttgtcata
    22501 cgctgtagcg tatactcatt tccaataaaa tgaacgatat cccctgccgt aaatttagca
    22561 taaggtccag gtgaaacaag tagttcttcg ttatgagaaa ttgcgataac tgtagcacca
    22621 gtcgcctgcc agatattcaa ctcgctaatc gaacggtcca gatgcattgc tttttctgat
    22681 aaagacaatt caaacggaag taaagggttc atcttatgta ttcgcttcgt ttgattgatc
    22741 agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc cacactcgcc
    22801 atgatttctg cttttgtatc ttggatagat tgtacatctt gatactgcaa taggaaatct
    22861 tttgcttttt ctttcgaggc aacaaatgcc ccactcccat gccgtacttc catgatccca
    22921 agatctacca gcacattgat agcctttcgg gtggtctcag gagaagcatt gtacgtactg
    22981 gcaagcgttg agcgagcatg gatcttctca cccactttat atcggttttc aacgatacgt
    23041 tcggctaaat caacagcaat ctgctgatag cgcgggcgga cgattttttt ctttccagac
    23101 attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta gcaaaataag
    23161 caaaaaaggg gagaacagcc tcttgcaacg actgatcatc gcttgatctg ttcctttttt
    23221 actgggcaag ctgtttgaca gtcatcacaa ttctttccgg attttaaacg agtatagaca
    23281 atcgctcctg cacttccaaa gattaataca cttaataaga atgtcgccat cctattcctc
    23341 tccttctaat gacaatatag tataaaactg gttcttggat tttggctggc ggaacaaaac
    23401 ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctgacgcta cctgtccacc
    23461 ctccaataag tgtcccaact gatagatcac aaaactaact aaataagcta aaccgcattg
    23521 atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga tagccccaat
    23581 tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag ctgctgcggg
    23641 agtatatgtg tgttgtagca cttgccagat ttctctacca ttttcggata cttccgcatg
    23701 tccaaacaaa ataccaaatg tgccaataat attttcttta gcaactaatc ctgtaatcgc
    23761 tgcgacagtt ccttgccaat tcccccaacc aagcggctga aaaacaacag ccaggattcg
    23821 tccgaaaaag gctaggatac tttggtcttc aggaacaggt tgagcataaa aattataaga
    23881 agagctaaac cagatcacga tactagaaac aaagataatc gttcctgctt ttttaacaaa
    23941 agacttgctg cggtcatagg tttgtcttag gatcgttgac cattgaggaa aatgatatgc
    24001 gggtaattcc atgataaacg gagctgtttc tccagcaaac aaacgcgttt ttttcaagct
    24061 gattccagag aaaataattg cagtcatccc aagaaaatag gcagaagggg ctacccaaga
    24121 ttggtgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg cagaacaagg
    24181 catgaatgtc gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa tcgtgcgagt
    24241 agccatgacc cctggtacgc cacatcccgt ggcaatcagc ataggaataa aggattttcc
    24301 tgaaagcccg aatttccgga aaagtcgatc catgacaaaa gcgatcctcg ccatatatcc
    24361 gcagtcttct agaaagctca agcataaaaa caatacgatc aattgcggta aaaatcctaa
    24421 tactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc attccgctac
    24481 ttgccattca gctaacagtc tgccaacatg ttctggtagc cacgttccaa acaatgtatc
    24541 attgatccaa tctgtcccca ttgttcctac tgtttgaata gccaagtaat aaatcagcca
    24601 catcacaaat gcaaagattg gcaaggctaa ccaacgattc gttgcaatct gatcgatttt
    24661 gtcactcatc gataattgga aagaatcatt tgacacgaca gatagtgtac ataattgggt
    24721 aataaattca taacgttcat tgactagaat cgtttcacta tcgtcatcca atagtttctc
    24781 tgtcaactgg atcagttgtt cgatttcttt ttgctgagaa tttgttatct catcatcctc
    24841 attcaccttc tgatctcttt caaaatattt cattgcatac cagcgtttct tgctactttt
    24901 ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgcggat cgtatgtaca
    24961 gggaacagga atagaaagcc gtttttttgc agactcctga atcttttcga ttaatttttc
    25021 gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac cataggaaag
    25081 cttttctagg ttgatttttt tcccttcttt tttgatgata tccatcatat tcagaccaat
    25141 gatcatagga atgcctaaat ccaataactg cgtagttaaa tatagattcc gctctaaatt
    25201 ggtagcatca ataatgttga tgatcagttc aggctgttca tgaaccaaat aatctcttgc
    25261 aatcacttct tcgggtgtat aaggagacaa agaatagatg ccaggtaggt cttgaatatg
    25321 aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc ccggccaatt
    25381 ccctacatat tgattcgaac cagtcaagag attgaacaag ctcgtttttc cgctgtttgg
    25441 atttccgact aacgcgatat tcattcgttt tcctcccttt gtaccaagac taattcagcc
    25501 tctgactttc gtaatgtcaa ttcatatcct cgcactgtca gttcgattgg atctcctaga
    25561 ggggctagtt ttcggatgac tatttttaca tttcgcgtca atcccatatc catcaaccgc
    25621 cgcttcactg cgccttgtcc gtggatggct acgacgatcc cagtctctcc tgcttttaat
    25681 tgatcaagag aggtccagct ttcagaggaa gcaatctttt tattgacaaa gatctgactt
    25741 agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc attgttgcct
    25801 gataaattga cgacgacgat tttacttccg gcaacgaccc caatattttg caaatggcgt
    25861 tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaagttttg ctttgcctga
    25921 gctaaggttt gcatattgac tcctctcttg tgaaataaat catgtgttga aatcattcat
    25981 tctatgttct agttttctca cgtattacgc aaataaatga gaatcattct cacatagaat
    26041 actctcggta attgagaatg tcaattacac ttgtgata
//
LOCUS       DADJYG010000019        25655 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.019, whole genome shotgun sequence.
ACCESSION   DADJYG010000019 DADJYG010000000
VERSION     DADJYG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.019"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..385
                     /locus_tag="JZ382_000718"
     CDS             <1..385
                     /locus_tag="JZ382_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426922.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..800
                     /locus_tag="JZ382_000719"
     CDS             399..800
                     /locus_tag="JZ382_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426923.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            802..1173
                     /locus_tag="JZ382_000720"
     CDS             802..1173
                     /locus_tag="JZ382_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426924.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1209..1511)
                     /locus_tag="JZ382_000721"
     CDS             complement(1209..1511)
                     /locus_tag="JZ382_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426925.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1760..1960)
                     /locus_tag="JZ382_000722"
     CDS             complement(1760..1960)
                     /locus_tag="JZ382_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2426926.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2265..3497)
                     /locus_tag="JZ382_000723"
     CDS             complement(2265..3497)
                     /locus_tag="JZ382_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY2426927.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3751..4320
                     /locus_tag="JZ382_000724"
     CDS             3751..4320
                     /locus_tag="JZ382_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2426928.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4498..4938)
                     /locus_tag="JZ382_000725"
     CDS             complement(4498..4938)
                     /locus_tag="JZ382_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY2426929.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5096..5860
                     /gene="map"
                     /locus_tag="JZ382_000726"
     CDS             5096..5860
                     /gene="map"
                     /locus_tag="JZ382_000726"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY2426930.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5892..6815
                     /locus_tag="JZ382_000727"
     CDS             5892..6815
                     /locus_tag="JZ382_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAY2426931.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6891..8030
                     /locus_tag="JZ382_000728"
     CDS             6891..8030
                     /locus_tag="JZ382_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAY2426932.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8023..8823
                     /locus_tag="JZ382_000729"
     CDS             8023..8823
                     /locus_tag="JZ382_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY2426933.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8823..9650
                     /locus_tag="JZ382_000730"
     CDS             8823..9650
                     /locus_tag="JZ382_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY2426934.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9628..10362
                     /locus_tag="JZ382_000731"
     CDS             9628..10362
                     /locus_tag="JZ382_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY2426935.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10462..11328
                     /gene="rfbA"
                     /locus_tag="JZ382_000732"
     CDS             10462..11328
                     /gene="rfbA"
                     /locus_tag="JZ382_000732"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY2426936.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11342..11914
                     /gene="rfbC"
                     /locus_tag="JZ382_000733"
     CDS             11342..11914
                     /gene="rfbC"
                     /locus_tag="JZ382_000733"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY2426937.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11936..12964
                     /gene="rfbB"
                     /locus_tag="JZ382_000734"
     CDS             11936..12964
                     /gene="rfbB"
                     /locus_tag="JZ382_000734"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY2426938.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13062..13913
                     /gene="rfbD"
                     /locus_tag="JZ382_000735"
     CDS             13062..13913
                     /gene="rfbD"
                     /locus_tag="JZ382_000735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY2426939.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13948..15981
                     /locus_tag="JZ382_000736"
     CDS             13948..15981
                     /locus_tag="JZ382_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426940.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16025..17305
                     /locus_tag="JZ382_000737"
     CDS             16025..17305
                     /locus_tag="JZ382_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAY2426941.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17515..18321
                     /locus_tag="JZ382_000738"
     CDS             17515..18321
                     /locus_tag="JZ382_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426942.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18333..19553
                     /locus_tag="JZ382_000739"
     CDS             18333..19553
                     /locus_tag="JZ382_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426943.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19543..21129
                     /locus_tag="JZ382_000740"
     CDS             19543..21129
                     /locus_tag="JZ382_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2426944.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21168..23306
                     /locus_tag="JZ382_000741"
     CDS             21168..23306
                     /locus_tag="JZ382_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY2426945.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(23674..24657)
                     /locus_tag="JZ382_000742"
     CDS             complement(23674..24657)
                     /locus_tag="JZ382_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAY2426946.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            25035..>25655
                     /locus_tag="JZ382_000743"
     CDS             25035..>25655
                     /locus_tag="JZ382_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAY2426947.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLV"
BASE COUNT         8159 a         4314 c         5205 g         7977 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag
      781 tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat ttccaaacaa
      841 attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc tgtttcaaga
      901 gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta tgatattatt
      961 tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt ttcaacagtt
     1021 aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga gaaaagaaaa
     1081 tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct gatgaatcga
     1141 gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt tgtttgttat
     1201 ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat atagtcataa
     1261 cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa aaagtataca
     1321 cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt agaatagtag
     1381 agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt ccatacttta
     1441 taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg tgtttttcct
     1501 tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct ttttgtgaag
     1561 gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta tgtgccaaaa
     1621 aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag atttcctgca
     1681 ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat aacccctgta
     1741 tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat
     1801 gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat
     1861 gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta
     1921 aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta
     1981 aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt tcttaactct
     2041 agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta
     2101 ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat
     2161 aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt
     2221 attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca
     2281 tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca
     2341 gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct
     2401 tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag
     2461 tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata
     2521 ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca
     2581 agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct
     2641 ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt
     2701 gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata
     2761 tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt
     2821 ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt
     2881 tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag
     2941 acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact
     3001 tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc
     3061 acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg
     3121 ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga
     3181 tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg
     3241 attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt
     3301 tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct
     3361 tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt
     3421 cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca
     3481 ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta actattagca
     3541 tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt
     3601 tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc
     3661 aaaaactaat tttgcatatc aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     3721 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     3781 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     3841 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     3901 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     3961 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     4021 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     4081 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     4141 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     4201 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     4261 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     4321 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     4381 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     4441 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     4501 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     4561 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     4621 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     4681 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     4741 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     4801 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     4861 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     4921 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     4981 taccaaaaca tcattgaaaa gaaaataaaa cctaaagaca cctggggaat gtcatttggc
     5041 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     5101 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     5161 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     5221 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     5281 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     5341 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     5401 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     5461 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     5521 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     5581 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     5641 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     5701 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     5761 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     5821 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     5881 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     5941 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     6001 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     6061 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     6121 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     6181 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     6241 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     6301 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     6361 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     6421 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     6481 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     6541 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     6601 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     6661 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     6721 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     6781 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     6841 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     6901 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     6961 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     7021 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     7081 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     7141 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     7201 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     7261 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     7321 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     7381 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     7441 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     7501 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     7561 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     7621 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     7681 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     7741 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     7801 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcgtat
     7861 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     7921 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     7981 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     8041 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     8101 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     8161 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     8221 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     8281 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     8341 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     8401 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     8461 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     8521 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     8581 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     8641 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     8701 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     8761 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     8821 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     8881 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     8941 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     9001 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     9061 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     9121 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     9181 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     9241 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     9301 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     9361 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     9421 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     9481 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     9541 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     9601 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     9661 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     9721 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     9781 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     9841 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     9901 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     9961 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
    10021 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
    10081 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
    10141 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
    10201 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
    10261 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
    10321 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
    10381 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
    10441 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
    10501 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
    10561 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
    10621 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
    10681 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
    10741 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
    10801 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
    10861 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
    10921 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
    10981 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
    11041 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
    11101 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
    11161 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
    11221 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
    11281 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
    11341 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
    11401 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
    11461 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
    11521 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
    11581 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
    11641 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
    11701 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
    11761 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
    11821 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
    11881 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
    11941 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
    12001 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
    12061 agagaacttg gccggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
    12121 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    12181 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    12241 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    12301 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    12361 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    12421 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    12481 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    12541 agtaacgagt atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    12601 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    12661 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    12721 attgatctta gaaatgatgg gccaaccaaa agacgcatac gaccatgtaa acgaccgcgc
    12781 agggcacgat ttgcgttatg caatcgattc gacaaaattg cgagaagagc taggttggca
    12841 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    12901 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    12961 ataatacaga ggaaatttca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    13021 tgtctctttt ttaaaataaa aatcaaggag cggataacca tatgttttta ttaactggag
    13081 gaaatggaca attaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgccatatg
    13141 tttcaacaga tgcaaaagac ttggatatca cagatgctga gaaaacaatg gcttatatta
    13201 cagaattaaa acccgatgtt attttccatt gcgcagctta cacagcagta gataaagcag
    13261 aagaagaagc aaaagaatta gatgagaaaa tcaatgtcga tggcacaaga aatgtagcac
    13321 ttgcagcaaa agctgtgaat gcacaattcg tctatatcag tacagactat gttttcgatg
    13381 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    13441 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    13501 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    13561 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    13621 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    13681 atcatttgtc gaacgaaaat agctgttctt ggtatgagtt cgcaaaagaa atattgaaag
    13741 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    13801 cgcagtattc agtcatgagc ctgaaaaaga cagaagcgtt aggttttgtc ataccaactt
    13861 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    13921 tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa
    13981 gaaaagccaa aagcgaatca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc
    14041 cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt
    14101 tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg
    14161 atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac
    14221 ctctcacaag ctgaagagga cttttcgttg gtgaacgagg attattcatt aaacggacaa
    14281 aacatgatca ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc
    14341 aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa
    14401 ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa gaggaaaaaa
    14461 gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc
    14521 atgcaacatg tgggcgattt gattttcttt actttgggac tgatgattgc tttttatcat
    14581 tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt
    14641 ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg
    14701 ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg
    14761 ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc
    14821 tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg
    14881 gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa
    14941 attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt
    15001 tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa
    15061 aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt
    15121 gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc
    15181 atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg
    15241 agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct
    15301 tatttctatg cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc
    15361 gcagttgctg tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg
    15421 ttttacctcg cttttgtcgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta
    15481 gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa
    15541 caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg
    15601 ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc
    15661 gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc
    15721 gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat
    15781 tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat
    15841 tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat
    15901 atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa
    15961 aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat tagtagaaag
    16021 gagagtgaag ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt
    16081 cttgcttttg ttgctgacgc tagtcttttt tgtttggaca gcaggggtag gttttccagc
    16141 aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct
    16201 cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc
    16261 ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt
    16321 actgtttcct ttggttattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg
    16381 ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt tatatcgtgt
    16441 atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc
    16501 tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg ctttattgat
    16561 cagtggattt gccagttctt ttgaacgatg gtatgttcga atcatcggtg ggatagcagt
    16621 cgttgcagcc tttttaggta tttgggtctg ccaatctaga ggtgcattag tcgcgttgat
    16681 tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt
    16741 tattgcctac acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc
    16801 tgaaaaagtc aatttgttta ccgatcgaga agatatttgg cataaattct atcagacgct
    16861 aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc agcgaggaaa
    16921 ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg
    16981 atttgggatt gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga
    17041 tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt
    17101 tatggaggat acattgactt catacccatg ggttccgatt atttatatcc tgttggctat
    17161 ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc
    17221 atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc
    17281 ttccaggatc caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt
    17341 cgcagccttt tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga
    17401 gaaaatcttg tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat
    17461 atggtaaaat ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt
    17521 aaagatttgt atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt
    17581 tcgatcaatg acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt
    17641 atcaatccgc tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca
    17701 caaatgatcg atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg
    17761 ttcttcttct cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta
    17821 gtaaagaaag tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg
    17881 tacacccatt tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat
    17941 ccttcagcaa tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca
    18001 ggattgactt ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt
    18061 acgatcgtgc tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt
    18121 gattcaggtc ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat
    18181 cgtgagtcat ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc
    18241 tgggttttca cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca
    18301 catttttcag atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag
    18361 atatcacaaa atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca
    18421 atccttttaa gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa
    18481 taaaaaaagg tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt
    18541 tgaaaatcat tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa
    18601 tctcagcttt gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata
    18661 tctatttaaa tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg
    18721 atatcattga atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa
    18781 gcgggatgtt cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga
    18841 ttgtggatga agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat
    18901 ttacagaaat caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga
    18961 ttcgtcgttt ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg
    19021 atacgatgga agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag
    19081 tggatcggaa taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga
    19141 aagcggaatt gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac
    19201 tatacgttaa agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa
    19261 ttgcgctacg aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta
    19321 tcaagattcc ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt
    19381 tgttagctgg tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt
    19441 tggtatataa gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca
    19501 tcgtggtctt agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa
    19561 atggacctag atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt
    19621 aaagttttag agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa
    19681 caatgtgaag tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt
    19741 gctcaagatg gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga
    19801 cagacatttg attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa
    19861 acattgaaga aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct
    19921 aacttggcgc ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct
    19981 tatggtctcc tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt
    20041 tttgaaaaag cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat
    20101 acagaattaa aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg
    20161 cgaccatttg gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa
    20221 agttccattg cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa
    20281 acgaaacaag atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag
    20341 acactaagtt tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa
    20401 cctagcttta tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg
    20461 aatgctgtag tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag
    20521 ttcgttctaa cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc
    20581 ggggaattga caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc
    20641 acaaagcagc tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag
    20701 gaaaatacaa gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg
    20761 gaaagtgatg aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa
    20821 aaaatccaag aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca
    20881 aaaatattaa aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat
    20941 aagttatcag ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc
    21001 aatcaagcag aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc
    21061 gagcaagaac gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt
    21121 gaaatgtaaa taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag
    21181 aaattgccgt atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg
    21241 tgggctgggc aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa
    21301 atgtgattgc tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg
    21361 atgttaagac ccaaagcggt ttcaaggtac gtttatcagg gaaaatgaga ggaaaagcca
    21421 tcttagactt ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat
    21481 acccttatga tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag
    21541 ggataaatta tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat
    21601 tgaagccagg atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga
    21661 aagagcaaag gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc
    21721 ctgtctataa cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa
    21781 cgtatgatca ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa
    21841 agtgtctgga atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa
    21901 atggacacat cagtctggca actaattctg cattggaaat agcagaagga gaatttatag
    21961 cactcttaga taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc
    22021 taaacgtaca tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg
    22081 gcaatcgctt tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata
    22141 attatatctc tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc
    22201 gtaaaggcta tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc
    22261 ctgcagatca tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat
    22321 caactgcaag taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga
    22381 cagatgccct gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat
    22441 tttatgagat tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga
    22501 agaatggcta tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt
    22561 caaattatga aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat
    22621 ttgcagaata caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat
    22681 tcaattattc tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat
    22741 tcttgaataa cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg
    22801 cacagtttga tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc
    22861 agcatgcagg agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg
    22921 atcgaacaca ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca
    22981 ctgcagcttg tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa
    23041 cattagaagt agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt
    23101 acaatgtcta tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg
    23161 aagatactcc agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat
    23221 ggccagcata tattgcgcat gatccatttt acaatgataa tttaacaaaa gacggaatcg
    23281 gcgacttttc gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct
    23341 cgcacataac ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa
    23401 aaataagtcg aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa
    23461 aattgctttt caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc
    23521 accgtttatt cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct
    23581 tattcatgct cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc
    23641 tgtcaaagaa tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta
    23701 ttcctctaca aaagccactc tatcaattat catgggttta aaataagaag tgatggagat
    23761 aaccagattg ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata
    23821 tatttcgttc tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc
    23881 ttctaaagca ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg
    23941 ttttccttta tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc
    24001 cgataaacac aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt
    24061 ggttttagga gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata
    24121 attgacatgg tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt
    24181 tgttagctta cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata
    24241 gttgaattcg ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca
    24301 atcttcactt gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat
    24361 tgttagcaag tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata
    24421 ctctggaaaa taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc
    24481 aatgccaatc agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc
    24541 ttttttataa ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat
    24601 ttgacatata tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag
    24661 atattacctc actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg
    24721 attcttcacg taatacaggt attcttatgg actagacagc gctttgggaa gatggattct
    24781 ctgaaaaata taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt
    24841 ctttaattta ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct
    24901 tgtcttgatt agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt
    24961 gcttataaac taggttttta aaggtgacta tggtaagata atagagatat tattttgtga
    25021 ttaggagtgt atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta
    25081 tattaatgga tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc
    25141 gtggagagat cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag
    25201 tgatttttat tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca
    25261 gtgacatcgt gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag
    25321 tgacttttgc gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc
    25381 tcggtacgct tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa
    25441 gtcgagataa aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa
    25501 attttggttc aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt
    25561 atgcaaacat caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg
    25621 aagaaactga aaaaatcaaa atctatcaat tagtt
//
LOCUS       DADJYG010000020        25482 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.020, whole genome shotgun sequence.
ACCESSION   DADJYG010000020 DADJYG010000000
VERSION     DADJYG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.020"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..2142
                     /locus_tag="JZ382_000744"
     CDS             82..2142
                     /locus_tag="JZ382_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426948.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2225..2794
                     /locus_tag="JZ382_000745"
     CDS             2225..2794
                     /locus_tag="JZ382_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2426949.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2969..3775)
                     /locus_tag="JZ382_000746"
     CDS             complement(2969..3775)
                     /locus_tag="JZ382_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426950.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3913..5427
                     /locus_tag="JZ382_000747"
     CDS             3913..5427
                     /locus_tag="JZ382_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAY2426951.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5830..6813)
                     /locus_tag="JZ382_000748"
     CDS             complement(5830..6813)
                     /locus_tag="JZ382_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAY2426952.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6818..7465)
                     /locus_tag="JZ382_000749"
     CDS             complement(6818..7465)
                     /locus_tag="JZ382_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2426953.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7550..8182)
                     /locus_tag="JZ382_000750"
     CDS             complement(7550..8182)
                     /locus_tag="JZ382_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY2426954.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="JZ382_000751"
     CDS             complement(8198..8749)
                     /gene="coaC"
                     /locus_tag="JZ382_000751"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAY2426955.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8746..9483)
                     /locus_tag="JZ382_000752"
     CDS             complement(8746..9483)
                     /locus_tag="JZ382_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAY2426956.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9480..10469)
                     /locus_tag="JZ382_000753"
     CDS             complement(9480..10469)
                     /locus_tag="JZ382_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY2426957.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10727..11269
                     /locus_tag="JZ382_000754"
     CDS             10727..11269
                     /locus_tag="JZ382_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2426958.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11286..12371
                     /locus_tag="JZ382_000755"
     CDS             11286..12371
                     /locus_tag="JZ382_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2426959.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12383..13189
                     /locus_tag="JZ382_000756"
     CDS             12383..13189
                     /locus_tag="JZ382_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426960.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13190..14017
                     /locus_tag="JZ382_000757"
     CDS             13190..14017
                     /locus_tag="JZ382_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2426961.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14014..15087
                     /locus_tag="JZ382_000758"
     CDS             14014..15087
                     /locus_tag="JZ382_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2426962.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15515..18211
                     /locus_tag="JZ382_000759"
     CDS             15515..18211
                     /locus_tag="JZ382_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2426963.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18288..18776
                     /locus_tag="JZ382_000760"
     CDS             18288..18776
                     /locus_tag="JZ382_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2426964.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18788..19282
                     /locus_tag="JZ382_000761"
     CDS             18788..19282
                     /locus_tag="JZ382_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2426965.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19303..20112
                     /locus_tag="JZ382_000762"
     CDS             19303..20112
                     /locus_tag="JZ382_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2426966.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20109..20930
                     /locus_tag="JZ382_000763"
     CDS             20109..20930
                     /locus_tag="JZ382_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2426967.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20981..23080
                     /locus_tag="JZ382_000764"
     CDS             20981..23080
                     /locus_tag="JZ382_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAY2426968.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23077..24195
                     /locus_tag="JZ382_000765"
     CDS             23077..24195
                     /locus_tag="JZ382_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAY2426969.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24234..25112
                     /locus_tag="JZ382_000766"
     CDS             24234..25112
                     /locus_tag="JZ382_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2426970.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25258..25455
                     /locus_tag="JZ382_000767"
     CDS             25258..25455
                     /locus_tag="JZ382_000767"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426971.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8043 a         4438 c         5406 g         7595 t
ORIGIN      
        1 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
       61 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      121 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      181 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      241 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      301 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      361 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      421 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      481 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      541 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      601 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      661 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      721 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      781 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      841 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      901 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
      961 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1021 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1081 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1141 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1201 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1261 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1321 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1381 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1441 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1501 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1561 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1621 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1681 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1741 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1801 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1861 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1921 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     1981 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2041 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2101 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2161 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2221 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2281 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2341 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2401 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2461 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2521 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa
     2581 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2641 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2701 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2761 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2821 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2881 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     2941 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3001 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3061 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3121 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3181 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3241 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3301 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3361 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3421 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3481 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3541 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3601 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3661 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3721 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3781 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3841 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3901 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     3961 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4021 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4081 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4141 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4201 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4261 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4321 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4381 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4441 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4501 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4561 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4621 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4681 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4741 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4801 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4861 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4921 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     4981 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5041 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5101 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5161 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5221 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5281 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5341 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5401 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5461 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5521 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt
     5581 taacttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa
     5641 gagcgtggga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     5701 gaagcagaag aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
     5761 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc
     5821 agcatgattt tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat
     5881 atattcatgc gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat
     5941 cttgccattt tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt
     6001 atactgatca cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa
     6061 tacaccaagg tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg
     6121 tccatcaacg gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa
     6181 aataacatcg actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt
     6241 tttatagttg atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga
     6301 tcctgcagta acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt
     6361 tcccacacca cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag
     6421 ccagtaaagt gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata
     6481 gtctagcgta tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata
     6541 accacctaga ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact
     6601 tttattctca actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa
     6661 agagactgat tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat
     6721 cagtaattcg ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac
     6781 agaaccagga tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt
     6841 ggaatgctgt attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat
     6901 ccgtttcttc cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg
     6961 atgccttctc attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg
     7021 tgactggttg aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat
     7081 gtttagtaat ataataatct gtcatggact caggccattt tgctggttca tctgcataaa
     7141 cagcactgac catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga
     7201 ttgcgccatc caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca
     7261 atacatcgat gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag
     7321 agacatacga aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg
     7381 caaaaatttc atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt
     7441 ttgcacctac aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc
     7501 aatagtaaca ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa
     7561 gagaagggtg cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg
     7621 cgacgattcc ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag
     7681 catttaaatt tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat
     7741 tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg
     7801 aatctgcttt ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc
     7861 caatcaagat gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc
     7921 cgatgactgg gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac
     7981 ggatcatgct gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa
     8041 cgatcgaagc aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga
     8101 taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg
     8161 taaagttttt tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag
     8221 tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc
     8281 cacaagctaa aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt
     8341 ccagattacg ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca
     8401 attttggtgt tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc
     8461 cgtttgctag cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt
     8521 gttttgctag ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat
     8581 ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt
     8641 tagacataat cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc
     8701 ttttgtaagc agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc
     8761 cagtctaatt ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac
     8821 tgttccatct ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc
     8881 atttgcgaaa ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag
     8941 agattccttt ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca
     9001 ttcacgtaga taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt
     9061 gttagaagag atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc
     9121 cagttcttct tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact
     9181 aaaagcagga gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt
     9241 tttcgttagt tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt
     9301 tatattggag tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt
     9361 atccggattg gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt
     9421 tgtaatagca cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt
     9481 catttatggt cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta
     9541 gatgctgttt caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc
     9601 tgcaaatccg ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa
     9661 tcaattccaa tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg
     9721 attcccccag cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga
     9781 ctgcttagct gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa
     9841 gcttttgttg tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt
     9901 tttagccatg tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt
     9961 ttatgtaaca gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact
    10021 tttttatcgg aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac
    10081 cctgctgtac caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac
    10141 cagtctcctg gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca
    10201 aaaccagtag caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca
    10261 gttccggata aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg
    10321 ctcattgcca atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca
    10381 ttttcagcca actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt
    10441 attacctgaa aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt
    10501 atcataccat aaagcctact cttttattct tgttcttccg taaatttaga caaaaattag
    10561 gtgaaaaata aagaatcaaa caactagaat ttattccaaa agataaagaa atattcagca
    10621 aaaaaaattt tacaaagggt tgcctttttc aataaatttc gtatactaat acgagtggta
    10681 gtaaacagga agtttactat tagaaaactt cgattcggag gaaattatgg aaattggtga
    10741 gaagttaaga aatttgcgca tccaaaagaa tttgacacaa gaagaactag gcgaacgcac
    10801 agatttgtca aaagggtata tttcccagtt ggaacgagat ttaagttcac catcaatgga
    10861 aacattcttt tctattcttg aagtcttagg tgtgactcca gaagaatttt ttcatcaaga
    10921 agcagcgaat ctgcaagtgg tttattcaaa agaagatcat acggtttatt tcgatgaaga
    10981 aaacgggtat gaattggaat ggctcgtggc tgattcgaat gaaaaagaaa tggagcctgt
    11041 cttgctgact tttgaaggtg caggagaata taagacgttt gaaccttcat tatctgaaac
    11101 gtttattttt gtattagaag gggaattaga gttaagctta ggagagacgg tctatgtggc
    11161 aaaagcagga cagtcgatct attatcaagc gacaaaacaa catcaattaa ggaatcatct
    11221 caaaaaacga acaaaagtat tgattgttgc aacgcaatca tatttgtaat atagggggta
    11281 aaaacgtggg gaaaccaatc atttcatttg atcaagtagt gaaacgctat gatgatgaaa
    11341 cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa attttataca ctgcttggtc
    11401 cttccggctg tggtaaaaca acgatcctac gtattatagc tggattcacc gaagctacag
    11461 aaggggacat ctattttgaa gggaaacggt tgaatgattt gccagctaat aagcgacaag
    11521 taaatacagt cttccaagat tatgcacttt ttcctcatat gaatgttttt gataacgttg
    11581 cttttggatt gaagatcaag aaaatgtcca aatcagatat cgaaaagaag gtaaaagatg
    11641 ctttgcgtat ggtccagctt cctggatacg aaaaacggga gattagtgaa atgtctggtg
    11701 gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa tgaaccgaaa gtattgctat
    11761 tagatgaacc gttatcggcg cttgatttga aactacgtac agatatgcag tatgaactgc
    11821 gggatctgca acaacgatta ggaatcacgt ttatttttgt tactcatgac caagaagaag
    11881 cgttggcaat gagtgatgag atttttgtca tgaataaagg gaaaatcgtc caaagtggta
    11941 cacccgtaga tatttatgat gagccaatca atcatttcgt tgctgatttt gttggggaga
    12001 gcaatatcgt ggacggtgta atgatcgaag ataatctagt tgagtttgtt ggtaagcaat
    12061 ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt tgaagttgtt ttacgcccag
    12121 aggatttgac gatcacgaca ccagataaag gaaaactagt cgtaacggtc gatactcagc
    12181 tgtttcgtgg tgtccattat gaaatcatct gttatgatga acagcaaaat gaatggatgg
    12241 tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg attggccttt gagccagaag
    12301 atatccatgt catgcgtttc aacgagtctg aagaagaatt cgatgcacgt cttgacagct
    12361 acgaagaata ggggggacaa gaatgaaaac aatgcgcagg atctattccg tcccttatat
    12421 tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg atcatttatc aatctttttt
    12481 tgatatgaat gggcaattca cgctgaataa ttatctatct tattttacgt cggggacatc
    12541 gctttctatg acgatcaatt ctgtatggta tgcatttttg atcacgtttt tcacgctgat
    12601 catcagttac ccgactgctt attttttaac aaaactgaag cacaagcaat tatggctgat
    12661 gctgattatt ttgcctacat gggtgaatct gctgcttaaa gcttacgcat ttattgggat
    12721 tttcagtgtg cacggcagcg tgaatcattt ctttgatttt atcgggatcg ggccgaaaca
    12781 gatattattc acagacttca gttttctgtt cgtggcaacg tatatcgaga ttccatttat
    12841 gattatgcca attttcaatg cattggaaga gatgaatcct tctttgatca gtgctagtag
    12901 agatttaggc gcgaatagct ttgagacctt ccgacgagtg atttttccgt tatcattaaa
    12961 tggtgtgaaa agtggtgtcc aagcagtatt tattccttct ctttctttat tcatgctgac
    13021 tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg gctatcgaac agcatttctt
    13081 ggttacacaa aactggggga tgggttctac aatcggtgtg attttaatca ttgctatgtt
    13141 catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt cgctcataaa tgaaaaaatt
    13201 taaatggtcg tatctttatt tagggatcgt ctttttattc ttatatgcac cgatcttcta
    13261 tctgattttt tattccttca atgaaggagg aacgatgaac agctttacag gatttacttg
    13321 ggaaaattat gaagctgttt ttgaagatac acgtttgatc attatcgtat tgaatacgtt
    13381 catgctggct ttcttatctg cattgatcgc cacgatcatc gggacatttg gggctatggg
    13441 aatctattac acgaaaaaac gacgtgcacg gacagcgttg ttgagcatga ataacatctt
    13501 attagtctct cctgatgtta ttatcggtgc tagtttcttg attttcttta cactactagg
    13561 attcggatta ggttttcaga gtgtattgct ttcccatatt gctttcagca ttccaatcgt
    13621 cgtcttgatg gtcttgccaa aattgcagga aatgaacgat tcaatggtcg atgcggctag
    13681 agaccttggt gctaataatt tccaagtagt caaaaatatc attttgcctt atctttctcc
    13741 aggaatcata gctggatatt ttatggcatt tacctattct cttgatgatt ttgcggttac
    13801 atttttcgtc acaggaaatg gctttacgac gctctcagta gagatctatt ctcgtgcaag
    13861 acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta gttttcttgt tctccatgat
    13921 attggtcatt ggatactact ttattagtaa agaaaaccgt ccaaaacggc tgaagaaaaa
    13981 cagaagagta cgcgaggggg tggcacggct gcgatgaaaa aattacaatc tctgttttta
    14041 ggtattctca tcattattgc ggtgctgctg cttggtgttc gtcaacttga aaaaacaagt
    14101 ggcatgagtg gagcaaaggt acttaccatc tataactggg gagattatat cgatcctagt
    14161 ttgatcacga aatttgaaaa agaatatgga tacaaagtga attatgaaac atttgattct
    14221 aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa actacgacat cgctatacca
    14281 agtgaatata tgatccaaaa aatgatcaaa gaaaaactag tacttccatt agatcattcg
    14341 aagatcaaag gattaaataa tattgatcca cgatttttag acttggattt tgatccagat
    14401 aatcagtatt cgattccata tttttgggga actttaggga tcgtatataa cgataaattc
    14461 tttgatgcaa atcaaattaa gcattgggat gatttgtgga agccggaatt aaaagacagt
    14521 ttgatgctga ttgacggtgc acgagaagtt atggggcttt ctttgaatag tttaggctat
    14581 tctttgaata gtaagaatat ggcacagctg acagaagcag caaataaatt gaatcattta
    14641 acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa tgtatatgat caatgaagaa
    14701 gcctccgtgg cagtgacatt ttctggcgaa gctgccgata tgatgtggga aaatgaacat
    14761 ttacattatg tgatcccacc agaaggatcc aatctttggt ttgataatat cgttatgcca
    14821 aaaaccgtga aaaataaaga aggtgcctat gatttcatca actttatgct agaaccggaa
    14881 aatgcggcac aaaatgcaga gtatattggt tattctacac caaatgaaga agcaaaagcg
    14941 ttattgccaa aagacatttc gtcggacgaa caattctatc catctgatga cacgatctcc
    15001 catcttgaag tctacaaaga tctaggatcg aaatacctag gcatctataa tgacctattc
    15061 ttagaattca agatgtatag gagataatgg caaaactcag taagaatggg gacttatcaa
    15121 cgttctaaag agctaaaaat cgtccttttt ctatctgagt tttccaatct tctaccaaaa
    15181 gcggatgaaa cgccgctttt tttgtgttat tttatttttt ctaccacgtt tttctaccag
    15241 agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac gggctagtaa atctaagatt
    15301 gaaaaagtta cagaaggata aagtaattgg aaactatgca aatatactta acccttctgt
    15361 aatctattaa taaaactaat atatgaatcc agttcaatat ttgtagctat gaaaaaacat
    15421 aaaaatcata ttagataaaa actaaattgg tagataaatt attttgtcag atatctgagg
    15481 caaatattcg actatttgtg tttcaaagta aaaattggaa tacaaatata gaatagacat
    15541 attgcagccg atagtttatt atgcttttag acacattaaa ctggaggaaa aaatgaaaaa
    15601 gagcgctaaa gatcgagttt ttgatgagct gatacgacta acagggaata tgtcttctgt
    15661 cactgccgag aaaatttcga agaatctagg tatctctcgt cagaatgcca gccattattt
    15721 gacgagatta gtggaagata aaaaggtcga gaaattaccg ggaaagcctg ttttgtggaa
    15781 gcctttagat gaatatgcgg ttgtagataa tacggagcaa ataaacgaag cctttcattc
    15841 tgtagttggt catgacggaa gtctgagaga ggtcattcaa aaatgtatag ccgctgtcaa
    15901 atatccgcct aacggactca gtgtattaat aaatggagcc acgggtgttg gaaaaagttt
    15961 tttagccaca aaaatttttg aatacgctat ccatgagcaa attattgaaa aagatgcacc
    16021 atttgctatc ttaaactgtg ccgattatgc ggacaatccc gaactgcttt cggcaacact
    16081 gtttggatac aagaaggggg cctttacggg ggctgaaaag gataccgatg gtttattggc
    16141 aacggccaac aacggctatc tttttctcga tgaggtccat cgcctatcga aagaaaatca
    16201 agaaaagctc tttctattta ttgatactgg aaactatcgt ccagtcggtg aaaatgtaaa
    16261 ttggcattct gctaaggttc gttttatttt cgccacgacg gaaaaaggtg aaaattattt
    16321 attagacacc tttgatcggc gtattcaaat tagtgttatg ttgccaactt ttgaagatcg
    16381 ccccattcga gaacgattag agctgattca attatttttt caaaatgaag cggatgtttt
    16441 acaaaaagat attattgttt caagagaagc cttgtcttta atgttatctc atccctttag
    16501 tgggaatatt ggtaagctga agaatatcat taaaatcagt tgtgcggatg tgtatagtcg
    16561 aacacaagaa gaaccattaa cgattgggaa aaatgagatc ttgattcagt taaatatgct
    16621 ggaatccgaa gtagaaagct tgccgattgc tagtttattg ataaagaaaa atcaaaagat
    16681 aaaaacggat cgaatcaaac cgtttgatta tgattcgatt ttggaaaaaa ttactcagct
    16741 gataaatgaa ggtaatttca atgaaaattt ttcttcgata aagaatgaag tacagcattt
    16801 ggtgcatcac attgataagg attctttttt atcaatgagt agaaaattga tgattcagat
    16861 gtttagaaag gtctgggtat ccgttttagg aaaaacgtac ggattgacga cgagtggacc
    16921 tgttgcagaa attattagca gatcctatgt catttcccca agctataaga cagacttaac
    16981 cgggataaat cgtacattag ctgcaaatct acctcggacg acttacttat gtaatcagtt
    17041 cttggagcat ttaccaccgc tctcagaaaa ggacgagaac tttctaagac tgcttattgt
    17101 cgtgacttta tctgattatg tagatgaatc catcgagctg aagggcctgt tgcttgctca
    17161 cggagatagt acggctagca gcattcaagc agtagtgaat caattatgcg gcaattatgt
    17221 ttttgaagcg ttggatatgc cagtagagag taatgtgtca gagattgtcg aatcgacaag
    17281 gaatttcgtg aaacggcaag tgaatacaga tagtctagtg ctgatcgtag atatgggctc
    17341 tttaaatcaa atttacacac agatcaagaa cgatttaaaa ggggagttac ttcttctgaa
    17401 taatttgacg acttccattg cacttgatat tggcttgaag atgacgatga atgcgccatt
    17461 taaacagatt gcggaggatg cagcagacaa gtattcaatc aatgttcaat actttgaagg
    17521 cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg ggtttgggga tttcggacaa
    17581 attgaaagat gtttttcgtc atcatttatc ggatgaatcc atcgaagttt tcacgaagga
    17641 ctacagagaa ctacgtgaat taatcgacca aaatgatgaa acttattttg acaagacaaa
    17701 attggtcatc acgacttcgg atcttccacg ttccttttca attcctaata ttaatgttta
    17761 tgacatctta gacggtgatg gagcagccaa tttggagcat attttgagtc ctgaaatgaa
    17821 gccggcagaa tttaaccgct tgattcagga gctagtaaaa ttcttttcgt tggaaggaat
    17881 tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg agtgaagtgg aaacagtcat
    17941 ttctaaatat gaaaattact atcatttcac tttaaatggc cgtgtaaaac taaatctcta
    18001 tatgcatacg gcattgatga tggaacgttt attcttggct cggacagaaa aatctgaaga
    18061 accgaattat gatttgaaac cagatgaaga tgaattttat tatgtttcta gaagtatttt
    18121 tcaatcaatg gagatgaaat ataattttaa agtcaatgct tatgagcttt cgctgctcta
    18181 tgaattatta ggaccgtata ttcaaaaata aagtgtctaa aaaagtgtct aatctgtttt
    18241 ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat taaaccaatg tttttaaaag
    18301 ttggcacagg aattgcttat ataaaagtga acagaaagaa aggaattaac catatgaaga
    18361 aaattctaat agcgactcac ggtcacttag cttcaggctt tcttagcagt attcaattac
    18421 tgacggggaa ggttcaagag attacggtga ttaatgctta tgtggatgag tgtgattttg
    18481 aacaggagtt gaatgtattt gtaaaccagg tgaacgagca ggatcagatt tttgttttta
    18541 ccgatttatt tggcggaagt gtcaatcaga agattaccaa aacctttttg gagaaagaga
    18601 ttgctgtaac tattttgtct ggctttaatc tgccaattct gctcgaaata gtattggcaa
    18661 ccaaggaatt aacaaaggag gatattcagg aactggtaaa taaatgccaa caggaattaa
    18721 agatgaccat tcttgatgag gtacatgtag taaatgaaga ggaagatttt ttttaggagg
    18781 aagaaaaatg attaaagcag tgcgaattga tgaacgatta attcatggac aagtagcgat
    18841 gacttggaca aagagtctaa atttaaccgg gttagtagta gcaagcgatg aggcggctac
    18901 caacgatatt caaaagatga cattgaagat ggctgcacca tcgaatgtca aagtcattat
    18961 taaaacagtt gatgacgcga ttcatttgtt aaaagatccg cgggcgaagg acatgcgctt
    19021 gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt gcgaaagctt tcccaacgga
    19081 aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca ccgccatcgg aagataagaa
    19141 aaccttatca aaggaagtga tgttaacgac gtcagaaatt caagacttaa aagaattagt
    19201 tgaactttat ccagataccg tttttcagcc tacaccggcg atggaaaaac gttcagctat
    19261 ctctattttg agtgattttt agaaaaagga aggagaaaaa ttatgtatga tgcatttgta
    19321 gtcgctttgg ccgtttttat tggagttgct ggacatgaaa tgttcgggat gtccatgctg
    19381 agtcgtccga ttgttgttgc tccgttagca ggagcgttga tgggggattt gcagacgggg
    19441 ttgattgtcg gggcgtcact agaagccatc tttatgggtg tcgtcaatat cggaatctct
    19501 agtacggcgg agccttcttt ggctgctggg ctggctacag cgttcgcaat caaaatggga
    19561 ggaaacgtgg atgcgattat tcccattgtc tttccactag cagtattagg attacagtta
    19621 atgaatatga ttttttcttt tgtatgtgga ccgatggctg ctaaattttt caaatttgca
    19681 aaatctggtg atcaaaaagg aatcatcaat ctgcacttct ttatgtggtt tgtccactat
    19741 gcgctttatg ctcttattcc attctttgcc gttttatttg gagcagatgt tgtcaaaagc
    19801 gtcttggata atattccaga ggtcatcatg aacggattaa cagtcgctgg aaatctgctg
    19861 ccggcagttg ggatggcgat gctgttacgg atgctttgga gtaaaaatat tgctgtgtgg
    19921 tttttctttg gggccgttgt tatggcgtat ctcgagttac cattgattgc aattgccgca
    19981 gttggaacga ttattgcaat tgttatagcc caaagagatc tacaactaaa aaatatatct
    20041 gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc ttgaccaaga agaggaggat
    20101 ttctttcaat gaaattagct gatggaattt ctaaagatga gaaaaaattg gttcggaaga
    20161 tgttttggcg ctcgatgact atgtatgctt cggtgaatcc ggtgacgatg ggcggcggtg
    20221 gtttcgccta ttctatgatg cccttcatta atcatttcta caaaagtgaa gaagatcgag
    20281 ctgcggcgct ggaacgtcat acacagtatt tttcgacgac cattccttgc gcgtcctttg
    20341 tcatggggat tgcggcatcg atggagaagg aaaattctga aaaagctggt tttgacgtgg
    20401 aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt ggctggaatt ggcgattcat
    20461 tgttctgggg agtttggcgt gtcgttaccg cgggactagc gatcaactta gccaatgccg
    20521 gaaatatttt ggcaccaatt atctttttag ttatgttcaa tatacctaat tacttgatgc
    20581 gatactatgg cggcgtttta ggctattcat taggctctaa atatattgaa aagctgtatg
    20641 cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat cttgggtctg atcatggtag
    20701 gtgcaatgac gatcagtatg gtcaaattca ataccgtttt gagcttttca atgaagggcg
    20761 aagaactaat gaatgtccaa gaaatcctcg atagtatttt taaaggaatt gtaccgttat
    20821 tggtaacatt gagctgtttc aaattactag ataaaaaagt cggcgtcatc aaaattattt
    20881 ttggaatggt cgtcttggga attttattgt cattacttgg aatcgcatag taggaagctg
    20941 cagctactag gctgcagttt ttttagcaag gagggaaaag atgactatta atgaagtaat
    21001 ttattcaccc aaacacccat tttttaatgc tgagttaaac gacggaaatg tgactacttc
    21061 aaacgaatac agctactatc ggaatattcc tgatgtaaag ggattgacct tcgatgtaag
    21121 taatcaaaag acattagcaa accttgatgt ttgcgggacg attcaaacgt tgacctttta
    21181 tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg gtcaataaac agctctctca
    21241 aaccaattcg ttatccttaa aggttgaagt aaacgggatt gtctaccatt tgtcagaatc
    21301 ggatcatcgt attgaagtcg atgtgattca agactgtttg cctagatata ttcatcatta
    21361 ttcgacgttt aaggtcattg tagtatcctt tgctcccata ctggctaata agcgtttaag
    21421 tatgcttgtt caacaggttt acattgtgaa tgaaacacac gaaagccttc aagtttcagt
    21481 gaaagatgtg ccttcgtatc aagagaagta ttcggatcag caaaatgtgt tgatttctca
    21541 aaaaggaaat aaggacacga tcgcacaaca ggaagtggca agcttcgcca ttgcttttgt
    21601 ggacccaaat gcttttgaag aagtaatgac ttttcaggaa tctgatatcg aaaaatggct
    21661 gaaggaaaca ttgaactatt ttgctcagct atttgggcag ctgtcaatgc caaacgatag
    21721 agttgtccat ctctatcgtc gtgcacttta tcaaagcttt tctagtttcg gtatgaatcg
    21781 acagggacag gtcgtcggca gcaattgggg cagctatcct gctactaatc gaatttggaa
    21841 taaggatatg tattacgcat cattgccatt caccatgttt gaaccggagt tatgtcaaaa
    21901 aacggttcta tggtttgatc aatatggcgt taagtttcct ggttcaaaat ttacaggagg
    21961 tatcaatcat tctctgagta attcgttatc cagtgcgtta ctggctagtc tttattttga
    22021 acatactggg gatacaaaat tctttgagcg gtgtccgcag gttctactga atgcttcaag
    22081 aattattgag gacatattag ctcaacgtcg gtcaggggaa ccaatgcttt ttccatctgt
    22141 ttggttatca gacgcctttg cacttggaaa gtatcatacc ggcagcaatc tttgtctttg
    22201 gaaggcctgt attggacttg cagaaatttt tgaagcccta gaacaactga gtcttagcaa
    22261 gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt tgtaaacaaa tgactatcga
    22321 tggtcctttt ggtgagcaat ttttggaggg aatcggtgat gaggagaaaa caacctattc
    22381 agtgaagcac tatcaaaaac cgattcttga gcaagggtta atctttttaa gtgatgtgat
    22441 tgtggatgga aaaattaatt tattgatgca tgatggagaa gaatcagaca ctacgttgat
    22501 gcctttttat caatttttag ataataaaga tcatttatat cagaacacca tggccttttc
    22561 tgctagttct ttcaatccaa cctatagcga ggaaatcaaa ggcatcactt ggggattaga
    22621 atccggcgcc acttttcctg gctttatcac tgttttaatg agcgagctgc acaaccctca
    22681 agctttcgta acccgattag aagaactaat ttgtttggca gatttagacg gttcgtggtg
    22741 gtggtggccg tatcgcttag aggctcaaca aggagaagtc gtgcgagact ttggctgcgg
    22801 caaatgcggc tgggcatcgg gtctttttgt cagcctaatg atcagtcaat atttcggatt
    22861 gaaattcaaa aaaggagtat tgcaaattga tcctgttgat aatttaacgt tttcttggaa
    22921 gaatttgaag ttcggtcaag ccttcatttc gattgaatgt acgcaatccg agttgagtat
    22981 ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg aaaaaaattc ttcacgtaga
    23041 aaagggaaaa acgattcaca ttcaaaggag aaaaagatga gtatactaaa aaatattacc
    23101 caatttaaaa aaatccagag tcgagcaatc actcccgaaa atccaacagg cgagaaaggt
    23161 aaagctgcga tgttgccaag cgagctagga ccaagccgca aaggtcgtgg cgcaattccg
    23221 ctgacttctg gtgaagaaac tgtgattgcg gatattactg gaacgggaat aattcgtcat
    23281 atgtggatga cgattcgtga tcgaacagaa tttggcagtt ttgttttacg tgatttaatc
    23341 ttaagaattt attgggatgg gtcggatagc cctgctgttg aaagtccttt aggggatttc
    23401 ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt tgccgatagt ggttaatcct
    23461 acagggggct ttaattccta tttcgaaatg cctttccaaa catccgctaa aattacgatc
    23521 gagaatttac atccgaagga tatgtcctca tttttttata cgataaatta cgctttggtg
    23581 gacgaattgg aagaaaatag cctatatttt cacgcaaatt ggcatcggga acgcattacc
    23641 aaattacagg aggattataa gttaattgat cgattaaagg gtcaaggcta ttacgtgggt
    23701 acttaccttg cgcttacctg cctagaacgt tattggtggg gcgaaggcga gtttaaattc
    23761 tacattgatg gggatagtga gtatccgacc gtaacctcaa caggttcaga agattatttt
    23821 ggcggggcgt gggcctttca tgagtacgat gagttcggac gtccacatgc gaaaaactat
    23881 tcaacagcct ttttagggta tccttaccaa tcaaatagag atgccacaag agatcgtttt
    23941 gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg acgcaatgcc acgacatggt
    24001 ctttatcgtt ggcatcttca agaccccatc tgttttaccg aagatttatc ggtcacttta
    24061 caacaaatcg gcaacgatga tgttcgctta tttgagcggc aagacgatgt tgctagtgtc
    24121 gcttactggt attcaacaga agcttcagga aatactaaac gtcatttcac tgatcgagaa
    24181 cgcttgccgc gttaatgaag tgtttttcac atattcgaag ggaggaacct atgatgatat
    24241 atggggcaat tgaagctgga gggactaaat ttgtctgtgc agtcggtact gaggaattaa
    24301 cgattttgga aagagtcagt tttcccacaa caactccagt tgaaacaatg aagcaggtca
    24361 ttgatttttt taaacctttt aaggaacaat tggcaggcat cggggtcggc tgctttggtc
    24421 caattgatat tcaatgcaaa tcaacgagtt tcggtcatat cacatcaaca ccaaaacttg
    24481 cttggcaaaa tttcaatttt gtaggaacgc taaagcaata tttcaatatt ccgattgaat
    24541 ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt tgctggttct ggcaaagggt
    24601 tagcaggtgt tgcatacttc actgtgggaa caggtatcgg aggtggtgcc ttgttcaatg
    24661 ggactttcgt ggaagggttc agtcatccgg aaatggggca tatgttgatc aagcgacatc
    24721 caaaggacaa tttttcagga aactgtccct tccaccaaga ttgtttagag gggatggcag
    24781 caggaccagc gattgaaaaa cggttgggtg taaaaggcca gaatttattg gctgacgatt
    24841 cgttttggca gattgaagcc ttttaccttg cccaatgtgc atacaataca acactgatgt
    24901 tttcgccaga tagaattatt tttggcggtg gtgtaatgaa acaagaacac atgaagaaaa
    24961 aagttcagga taaatttgtt gagcttataa atggatatgt tgaaatacca ccaattgata
    25021 gctacattat tacgcccgaa ttaggtgata atgccggaat aatcggcgga ttagcattgg
    25081 ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa tgtttcattg atactcatgt
    25141 caaaagtatt taaccgagta atcaaatgat caaatgaagt tggataaagc gaaaatgaat
    25201 tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat taaatcactg aaaggaaatg
    25261 cttctgcgtt tccttttttc tttttgttgt gcgctgtata ccttgaggat attcactggt
    25321 ggcgtacact taaatagtaa tatattgtgt tactttatgt ctgtggtaat agttatagtt
    25381 gcttaccaat gtatgttcat ttttcgaaat gttgacttca taatccttgg cttaatgttt
    25441 gaattttttt ggtgatagat tttttcagcc gtataaaata aa
//
LOCUS       DADJYG010000021        25379 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.021, whole genome shotgun sequence.
ACCESSION   DADJYG010000021 DADJYG010000000
VERSION     DADJYG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.021"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(84..1058)
                     /locus_tag="JZ382_000768"
     CDS             complement(84..1058)
                     /locus_tag="JZ382_000768"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAY2426972.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1138..2013)
                     /locus_tag="JZ382_000769"
     CDS             complement(1138..2013)
                     /locus_tag="JZ382_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAY2426973.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2143..4050
                     /locus_tag="JZ382_000770"
     CDS             2143..4050
                     /locus_tag="JZ382_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAY2426974.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4591..7869)
                     /locus_tag="JZ382_000771"
     CDS             complement(4591..7869)
                     /locus_tag="JZ382_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAY2426975.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8140..8685)
                     /locus_tag="JZ382_000772"
     CDS             complement(8140..8685)
                     /locus_tag="JZ382_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY2426976.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8697..9389)
                     /locus_tag="JZ382_000773"
     CDS             complement(8697..9389)
                     /locus_tag="JZ382_000773"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY2426977.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9408..9692)
                     /locus_tag="JZ382_000774"
     CDS             complement(9408..9692)
                     /locus_tag="JZ382_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2426978.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9705..10268)
                     /locus_tag="JZ382_000775"
     CDS             complement(9705..10268)
                     /locus_tag="JZ382_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAY2426979.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10496..11158
                     /locus_tag="JZ382_000776"
     CDS             10496..11158
                     /locus_tag="JZ382_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAY2426980.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11282..12478
                     /locus_tag="JZ382_000777"
     CDS             11282..12478
                     /locus_tag="JZ382_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAY2426981.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12579..13955)
                     /locus_tag="JZ382_000778"
     CDS             complement(12579..13955)
                     /locus_tag="JZ382_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2426982.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13992..14858)
                     /locus_tag="JZ382_000779"
     CDS             complement(13992..14858)
                     /locus_tag="JZ382_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAY2426983.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14947..15972)
                     /locus_tag="JZ382_000780"
     CDS             complement(14947..15972)
                     /locus_tag="JZ382_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2426984.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15990..16664)
                     /locus_tag="JZ382_000781"
     CDS             complement(15990..16664)
                     /locus_tag="JZ382_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2426985.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16848..17741)
                     /locus_tag="JZ382_000782"
     CDS             complement(16848..17741)
                     /locus_tag="JZ382_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAY2426986.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17815..19512)
                     /locus_tag="JZ382_000783"
     CDS             complement(17815..19512)
                     /locus_tag="JZ382_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAY2426987.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(19505..20818)
                     /locus_tag="JZ382_000784"
     CDS             complement(19505..20818)
                     /locus_tag="JZ382_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY2426988.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPQAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESSEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMDELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(20818..24855)
                     /locus_tag="JZ382_000785"
     CDS             complement(20818..24855)
                     /locus_tag="JZ382_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY2426989.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFYTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVYETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
BASE COUNT         7148 a         5579 c         4503 g         8149 t
ORIGIN      
        1 aaaaaggagc tgtgacaaaa gtcttgtcgc agctccttga agctgctcac ttctgtcaca
       61 acttctttcc ctcttttttt tgtttattcg aattcttttt cttccagacg gaataacgga
      121 ctcatcggtg tattttcatg gatgcgtttg accgcttcac ccattaattc tgaacatgta
      181 acgattgtta agttttctgg atgacgattt tcttctgtga aaacagaatc agtgatacaa
      241 atatctttga ttggtgcatt gtctaaaata cttttcgcat gatcggacaa taatccatgt
      301 gacgcacagg catatacttc ttttgcgccg ttttctttta atacatttgc tgctcgtgca
      361 agcgttgctc ctgtgttcaa gatatcatct accatgatac atgtctttcc ttgaacatca
      421 ccgatcacat aaccaccctc agttgaagca tcatctgcat gatctacgat ggctaatgtg
      481 ctgttcaaat attctgaaag actacgtgca cgctgaacac cgctgttttt tggcgaaaca
      541 actacgatgt catctcccgt cagacctaat tgacggtaat aatgagcaaa caacggcatc
      601 gtaaataaat tatctacagg aatatcaaag aatccttgta cttgaaccgt gtgcagatct
      661 aaagtcagta ctcgagttgc ccctgcttct tcgatcaagt tagcgatcaa tttcgcagtg
      721 atcggttcat gaggtttcgc cgtgcggtct tgcctagcat acccatagta aggcaatacg
      781 acattgatcg ttttcgcact tgctcgcttc attgcatcga tcatgatcag tagttccata
      841 tagtaatcat tgacaggtgc attcgttgat tgaacgatat acacatgatc ccctcgaata
      901 ctttcttcga tattgatgga aatctcgcca tcactaaatt gcttcacgac gcttttacct
      961 aattctgttc cgaacacttt cgcgattttt tcagctaatg gacggttgcc attcaaacta
     1021 aaaatcttca acgtgtcatc ctgatactta ttactcatgt agtcctcccc aaaacttctc
     1081 tattcttcga tagtttaaca tatttttcca agattttctg ttaatttcag caaattctta
     1141 aggaagtgaa actgttccgt tactccaact ggcaggaatg actttatgat aatcgcccca
     1201 gttgtcgtgt actttgtaat aacgttctcc tgtttcagtt tcaaaataag cgtacgctgt
     1261 cacccaatga ttgccatatg tactgccaag tactttcaaa ataccaatca tcacaggttt
     1321 gccttcacgt atacgttttg tagctcgctg ccatgaacct agaagagttg ctcgtgcacg
     1381 ctcatgattg ccattcttct ttaaaaaact agaaatccct gtactaacct gaaaaggaat
     1441 cgtaggaagt cctagtggtt ggatctggct acgcaatgcc ttcgtcaaaa ccaacgaatc
     1501 agccgtattt ttcttgcgaa tctcatttgg aagcatccac cccttacgat agtcttgata
     1561 ataggcaagg agaactgctg ccgcgtaagt accacaaaga tagcctgagg gggtagccca
     1621 tttcttatac ctcaaaaaac gttcttctgg caatcccacc cagcaatttg cgagttcctt
     1681 tcctgacctt ttttcttcga tcaaaacggg aatatcttct tttctgttga tccatctaga
     1741 taactcgatt ggctcttcat tgatcagctt ccatttttta caagaaagac cctcttgata
     1801 agtatatttt gctggagaat attctaccgg atgtcctgat tgatcgataa tcacataact
     1861 ttgatcttca aaaaccagag ctaaaaaaag caatttactt tttcttgact gcagctgctg
     1921 agtgctggct aattttttaa tttttttatc tactttgaaa tataaaggtt tatccactac
     1981 ttgattcagt tgggatcgcc tccttttttt cattataagt gaaggattga aaaaaacaaa
     2041 tcagtttgat tgttcgaatt ttcaattttt tatgttctat ttgttaattc cttcattttg
     2101 taagataatg ggcacacgaa acaaaaaagg agcgatcgat gaatgaacaa agaacgattg
     2161 aaacaagatt tctatgaagc agtcaatgaa gaatggcaaa aaacagcaaa aatccctgca
     2221 gataaaccag ctacaggcgg tttccaagat ttagttgacg ggatcgacca acttttgatg
     2281 ggagaaacag acgatctgct tgcccatcca gaaaaagtgt cttcagaaaa aatgaaacat
     2341 ttccttgcat attatcaact ggcaaacgat tatgaccgaa gaaatgagtt gcatgctcaa
     2401 ccgcttcaac ctataatcga aaaagttgaa tcattgcagt ctttcgacga attgaataag
     2461 caatttccag aatggctgtt ggatacatta ccattgccat ttaatctgga tgtagatgca
     2521 gatatgaaga atgcgcaaac gaacgccttc tttgcttatc cgccttcttt attccttcca
     2581 gataagacgt attatgcatc agaccatccc aacggtgcac aactaatagg cgttttcttt
     2641 gatatggctg ttcagttatt agagcttgcc ggcaaagaaa aaagtcaggc agaagcaatc
     2701 acggaacaag cagtgcaatt tgataaattg attgctcctc atgtaaaaag tgcagaagaa
     2761 aacgcagatt acagcaaaat gtataatccg caatcttttg atacatttat taaatacaca
     2821 gataaactgg acttgaaaaa acttgtcacg ggtttgattg ggacaactcc agataaagtg
     2881 atcgtaactg atccagtgta cttcgatcat ttgaatgaca tcctgacaga agctcatttt
     2941 ccgttattca aaagctggat gatcattaaa acagtacgtt ctcttagtgg ttatctatca
     3001 gaagaattcc gccaagtcag cggaatctat tctcgcgtat tgtctggaac agacgaagca
     3061 atgcctcaga aaaaagcagc gtattacctt gcctctggac aattcgatca agtagttggc
     3121 gattactacg gaaaaaaata ttttggcgaa gctgctaaaa aagacgtaga acaaatggtg
     3181 aaaaaaatga tcgccgttta taaaaaacgt ctagaagaaa atgactggct tggggatgca
     3241 acaaaagaaa aagccgtcat aaaattaaat aacttaggta tccaagtagg ctaccccgat
     3301 aagatcccag cattattcga ccagttcaaa acagtacctg cagatcaggg tggtactttg
     3361 ctttcaaata ctcttgtttt cagcaaactc acattaaaag atcgtttcag caaatggaac
     3421 aaaccagtgg atcgttcaga atgggaaatg agtgctgaca cggtcaatgc ttattatcat
     3481 ccatttcgga atgtgatcgt ctttccggca gcgatccttc aagcaccatt ttacagtcta
     3541 gaacaatcaa gctctgctaa ttatggtgga atcggagctg ttatcgctca tgagatttcc
     3601 catgcatttg ataataacgg cgctttattt gacgaatatg gcaacttgaa caactggtgg
     3661 acagaagaag atcttacaca atttcaaaac aaagcacaag caatgatcga agaattcgat
     3721 ggtctgcctt ttgctgacgg aaaagtaaac ggaaaattaa ccgtttcgga aaatattgct
     3781 gatgcaggcg gtctaagctg tgcattggaa gcagcaaaaa cggaatcaga tctttctatc
     3841 gaggaattct ttgtcaattg ggcaacgatt tggcgtacaa aagcaaaaaa agaataccaa
     3901 caacttttac tgcaaatcga tgtccatgcc cctgctaaac tccgagcaaa cgtgcaacca
     3961 aaaaaccttg acgaattcta tgaaacattt ggtatcacgg aacaagacgc tatgtatcta
     4021 gcgccagaaa aaagagtgcg aatctggtaa gaggttgtgc cagaagtggt cagcttcaag
     4081 aaataagaag gaatttggga aaatcgcttc tcaaattttt gtgacatttc ggcttatttt
     4141 tcgaagaagt tacttctgga ataccatgta ttagaggttg tgaaaaagct gcttagtttc
     4201 aagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttac
     4261 taccgagaaa ctggcttttg aacaccgttt atcgagaggt tgtgaaatca gcgtcctgac
     4321 ctgagtatta gagaaaaaaa cggaaaaata gttcctcata ttttgccgtt tgttgagcta
     4381 ataccgaggg tcagctgatt gaacaccgtt tagtggagag attgtgaaaa agctgtcctg
     4441 catcaagtaa taagaacagc caatcaaaag cagcaaggag aacctcatct ataaaaagat
     4501 agaaggaagt gagacatcta ttggtctcat ttccttctat ttttactttc ctcagtcaat
     4561 tgttttacat cataaaagga gaaattccac ttattttttt cgctgtttca tgacataaac
     4621 aactacaccg attaacaaaa ttcctccagc taatgctagc aacgggttat taaattctcc
     4681 cgtttttggg agtccttgag tgccattttt ttcactttct tttgctgtcc tatccggatg
     4741 agaaggtttt ccatttggtt ttccatttgg tgattcattt cctggatttt ctctttctgg
     4801 tttgtcattt cccgggggca catcggcagt ttctcgcaca gtgatcgctt tttgctgggt
     4861 aacagattct gtatgagcag accatttttc cccatcaaat atttccgaac gataagtgac
     4921 ttctagcgta tattctcctt ttgaaggtgc tgaaaaaata gcggtatgat tagaaaattg
     4981 agcacttgac tgtgtttcaa cgatttggat cttttctggg aaataacgca tatcttcaaa
     5041 gacaggcgtt ccttgatttg gtgtgaaagt tgacacaaaa actgtaaaag gcttcagttc
     5101 aatgatattt ccttctgcat tctccgccac accatttgct tcttgtataa taatcggatt
     5161 attttgttca tttggttttg tctgatttgg ttcttgtacg attgcatact ctggatattc
     5221 cgaagacggt gaggtttctc cgtcaaaaat agcacctaca gataatggaa gaccatccac
     5281 tctttccttt tctgtttgac gattccccgg tttaatctgc atcagtccat gaacatggat
     5341 acttccattt tcatagcgcg taacaggctg ctgaccttct ttcgaacatg tatttagaga
     5401 ctcgatccaa tcttttgata tggtatgtcc aagagtattt tctgattgtt tctctcgttc
     5461 attccaatag tagttcaacg agtggtagat ttgatcttcg ttttgattca atgggcggat
     5521 ctgctctctc atctcaagat tctctgttcg gaaagataag ataccagaag catggtagtc
     5581 tgtgtttcct gctgaagagg tataaagagc cacattatat gaaccgttat taaaacttgt
     5641 actgctattg acgataatat ctgttccgct attggaatcg attcctttcg caagattttc
     5701 gaaagacaaa ctattgatca attcatgagg gcccgttaaa gaagaaccgc ccattttgaa
     5761 tccattaccg ttaccttctc cctcaattcc tggtacccag ccgtttcgat aagcaactga
     5821 gtttttgatg attacttttc cgatcgaacc tgtttcattt ttagcaaaaa ggtcccaacc
     5881 gtcatctgca ttatgatacg cgatacaacc gtcaaataca tttccttctc ctattgtcag
     5941 tttggccgca aatccatcgg catcttcaaa tcctggatcg gcattttcaa aagatgtaca
     6001 gttcttgact aagttatgcg cgggccatgc ttcgaaagga tcatttgagc taccgctgat
     6061 ttggattcct gtatttccat tccgataagc atgaatctct tcgatcaaat tatgatttcc
     6121 agaaatctgt aatccttttt gcatagctgc actatttgta atgtcaaacc ctcgtaaacc
     6181 ccagtagtga ctgaacagtg tcatcccatt tccagttcct ttaaaatcaa gtacaggacg
     6241 cttgttttct ggatttttct ctgcaatcaa ctgaatcggg ttttctcttg tcccgttaac
     6301 gccttttgga atcgtcaatc ctctttcgaa ggaataagtt ccaggcgcca tgacgattgt
     6361 ctgcccagca tatgcgtatc tcaatgcttg tgtcagagat aatggatttt gtctgcttcc
     6421 gtttcctgca tctgaccctt ctggtgttac ataaactgtc gaatatttag gtttacggta
     6481 gtccactgta tgaagaatct cttttgtttc ataactagtc atctctacgt attcttctgg
     6541 cggatagttt ttatctggcg taaaactgaa agtaaattcg ttcgttcctt ccatcagttt
     6601 tgtctgataa tcaaattccc gattcgcagt gatagccaag ttttttgcgt tcttaagcac
     6661 ctctcctgtt gctttatttt taattgtcaa taatccgtca gcatttgctc taaaggacat
     6721 agtatgatct tggtttcctg aatgattact agaggtcaca gtgtaaatcg gatcaatcaa
     6781 gcgagtaggt ctttcttgcc caggaggatc tgtcgcagga tctgagtaag caacttcgat
     6841 tccttctacc ttgatcgtca tattccttgc tgcagcaaat cctgcataaa taaccgattc
     6901 atctgaagcg tataattttt cccagtcata catgatgtca gaagccattt cttctcctgt
     6961 ttcaggatcg taataatatg cctctaaacc agtattcgtt tttttcatct ctacgtccag
     7021 tttagtaatc gagttcgtgg ccggaaacag attcccatga ccgtttccga atatattata
     7081 agaacctgtt tctttttcca agaaccctgc tgaagtctct aaaggtgttg tttccactga
     7141 gacactccca ggagctggcg cagcttgcgg atcttcagat gatattcctg tgattgcccg
     7201 tgtccccaat ccaagtcgca tcgtatattt gctgccgctc gtgttgacca cttcttgtct
     7261 atccgagtcc cagacatatt cgatccgact gcctagtatg gcaaatgaat ttgaataaaa
     7321 tgcctgccca aacaaatgat tttttggtac tgcgtctctt accattaaag caaatccttc
     7381 ttgggcattc gtataggtcc aagattctat ttcgatcgtc gcccgcatcc taaagttgat
     7441 gtctttgggg attgcttgat aataataggt cattccatct gatccgcttg actggaattt
     7501 tccgccgtta ttcgtggaag agagccgcac gccgtcttct aatggttcaa tggcatacct
     7561 actagcactt gtgctttctc cgaaaacaac ccctttccac tcttcatttg cttcttcaga
     7621 ttgctttttt gtcttttcat tttctgattc tttcatcata ggatcatttc cttcttccgt
     7681 atccatatct tcactagttc ttggtatttc ctccattgtt gacatccctt caatgttcac
     7741 gtgtgcattt tcttcgttca cagactgttg ttcgttagca gcaacttcaa cgacagaaat
     7801 taaaaccggg gtactgagca taaggagatt catcattagc gcactgtatt ttatacgttt
     7861 ttttaacaag tgtattcctc ccgtaacaaa ttagaaagcg tttacttaaa gtattaccta
     7921 atttattttt actgtcaata aaaaacaaaa ttataataag tgtattttat aaaaagaaaa
     7981 aatataactt aagttgttga taataaagag atttattaac gtaaaaagaa taagtttttt
     8041 taaaataacg tataaaaaag agcataaaac aaagtcgttt gttttatgct cttcttttat
     8101 tattttgtgc tttttatttt atcaaatcac tttgtttttt tataaaattt ctgcgcctag
     8161 tgtggactca aaatgtctca atgcccattc atgtccaacg ggatcgaaac ttgccacggc
     8221 atctttgaaa atatggagtt tgtatcctag attatacgca tcgactgctg tatgcaacac
     8281 acagatatct gtacatacgc cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg
     8341 gatgtctaga tctgttccac taaaagcgga ataatgccgt ttgtctatcc aataaacatt
     8401 ttcctcttgc tcatagtctt ggtataaact ttttaaagag ccgtacaatt cccgtccacc
     8461 cgttcctatc acattatgcg gaggaaataa gcggttctcc ggatgaaagg aatcttttgg
     8521 atcatgcgca tcaatggcaa aaacgacaaa atccttttga tcaataaact tctttgtatg
     8581 agcagtcagc tttttttcaa ttacttgtcc tgctgtccca gtagtcagct ttccatcctc
     8641 tgcaacaaaa tcatatgtgt agtcaatcga gatcaatgcg ttcatcttag tcctccttat
     8701 acgagatgtt tgacaaattc gatcaccatt tctgctgatc gtttgcctgc gtcttcgata
     8761 aattcgtcaa agctttgtgt cgccgagtga tctgctgtgt cgctcattgc acgtacgatc
     8821 aaaaatggga tattgaactg acgagctgtt tgtccaacag cggctccttc catttcgcaa
     8881 gctaatgctt cagggaaatt cgttaagatt tctttgattt tatctggcga gtcgacaaat
     8941 gtatcacctg taacgatcaa tccttctttt gcgttcaaat tcgttgcttt tgctgcttta
     9001 accatttcac tgcgcaaata tgtgcttgct tcataatata gaggcattcc tggtaattgt
     9061 cctgggttgt agccaaatcc tgttgcatct gcatcaaaat aagctacttt gtctgatata
     9121 acgatgtcac ctacttgtaa gccttcaccg attccgccag ctgaacctgt attgatcacc
     9181 atgttcacgc cgtattgttg gatcaacaga cttgtggtaa tagatgctaa gacttttcct
     9241 ataccggaac gtacgacgat cacttcgtgg ttacctaatg aacctgaaat aaataaagcg
     9301 cctgcgcgtt cccatgataa tggttcgctt aggttctctc tcaaaatttt tacttcttct
     9361 tccattgcgc cgataatacc gattttcatc tttttaattc cttctttcta tataaatgcg
     9421 attgctaaaa aaaccacaca taacattagg ataacaataa taagagattt catcaaaaaa
     9481 gagttccatt tggtcgtctt ttcccgttcg ctgatccttg ttttcgtgat tggtttattc
     9541 tttttgcttt ctttacgata aaagctggca atcttttttt cttcttgctg ataagctgtt
     9601 tctgcttttc gctgtttttt taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt
     9661 tcgcttcgcg taataagcgg tcctttggcc ataagccgat ccctctactt tcctttggtc
     9721 aatagttccc agtattgaat cgcataaatc gtctttgcat cacaaatcaa ctgatctttc
     9781 atcgcttgtt gcgcctcttc aagagttaga tgatacaact ccagtacttc gtcttcatct
     9841 tgtgctaaag gattctcgac cttctctacc ccttgtgcat gataaatatg aaggacttca
     9901 tttgcaaaac ctggtgaaag atacatcgaa gtaagatggc tcagcgactt cgcacgatag
     9961 ccagtttctt cttctaattc tctagctgct gtcatttcgg gattttgtcc ttctcctgga
    10021 tcgatttttc cagctggaat ctccaaaatc actttttcta aaggttttcg aaattgtttg
    10081 acaagaagca gacgatcttg ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat
    10141 accagttctc tcttcgaagt tcctccatct ggcaaacgaa cttcgtctac agcaacatca
    10201 ataatttttc cgctatagat ttctttgcga ctgattgttt tttcttcaaa ttgctcattg
    10261 ttcagcatat cttctcctgc tttctttgta atcataactg cttttttttc atttttcaat
    10321 ttttctcaaa aactccttat tagtttacca tagataaacc ttagattttc tctaaatata
    10381 gaaaatcctt ctttagtccg atactctttc tagtagtccg tatgctaaaa taagtatgta
    10441 atcgaataac gaaaagaatt caatactcaa cgataaatga catggagggt ttattatgat
    10501 aaaacgaaat tggccgtggc ttctagcatt ggtcttgcta tttctctttg tccgaaacaa
    10561 tagtttttct tttgttctag gacttttatt gataggtgta gttttagttt tgtggggact
    10621 ctttggttcg cgcaggaaaa agggaaaaca agaagaaatt cctgcactga ctgacgaact
    10681 tgaaactcac tatgaaaaaa gcggtatgac agcgaatgaa atttcatttt ttcgacaaac
    10741 aatgaatcaa acaaaaaatg agatcaatca attgcaaatg aatatgcagc agacagccaa
    10801 acttaaagca atcgatctcc gccatgatcc agtcaaagca gcgaaagctt tattcaaaga
    10861 attggtgaaa gaaccaaacc gactgcatga agcaagtcag tttctttata cgcatctgcc
    10921 gaatctagtt gatttgacaa ataagtataa tgagatcaat gaccatgaga tcaaaaacaa
    10981 acagacctat gaaaaactag aagaaagcgc gcagatcatc gaccagctgt cagctttaat
    11041 cgcacaagat tatcaaaaat tcgttgctga ggatctagat gatatcgacg tagagatatc
    11101 tgttgcgaaa cagagcttaa agcgtgataa taaatatgaa ccagaacaaa aggattaact
    11161 cttttgttca taaaagaaga ctcggaacat tcagatgatg tctggctgtt tcgagtactt
    11221 tttctaaagt agagacattc atgtaagaat cgagaacaga ttcaacaagg aggaattttt
    11281 tatggatcat aaaccgactg aaacaaaaga tgttacacca gttaacgata cacttgaaga
    11341 tttattgaac aacccgtttt caactccaac agatacgctg accacttcac aacaagcgga
    11401 gattgatcag ctccaagagc aacagacagc tgctcgttta attgacaaac taccggcgga
    11461 acgccaagaa caagctaaac agctagcagc aaaaatcgat gctaatgatg cacagtctgt
    11521 catcagctac ggatcggctg cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa
    11581 tcatgtgcaa gcacaagata ttggtcctgt aggagattcc ttaactgaac tgatgtatcg
    11641 tctccaagaa gccaatcctg acgaactgcg tgccggagaa gggaacatct tccagcgtgt
    11701 cttcgggaag gtcaaacaat cgatctatga agtgacagca aaatatcaaa agattggtgc
    11761 acaaattgac aagatttcag tgaaattaga taaagaaaaa gacggtctgc taaaagataa
    11821 tctgatgtta gaacagcttt accagaaaaa caaagactac tttgatgcac tgaatattta
    11881 tatcgctgct ggagaattga aaatggaaga acttcaaaca acgatcattc cagaagcgat
    11941 gaaaagagca gaagaaactg gcgatcagat ggatgttcag attgccaatg attacacgca
    12001 attcctagat cgtctggata aacgtacgca tgatcttcgc ttagctagac agatcacgat
    12061 ccagcaagca ccacaaatcc gtttgatcca aaacacaaat caggccttgg ctgaaaagat
    12121 ccaagcatct atcgctacag ccattccgct gtggaaaaac caagtcgtga tcgcattgac
    12181 cttgcttcgt caaaaagatg cagtaactgc tcaacgccaa gtctctgaaa caacaaacga
    12241 cttattgaag aaaaattcag aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa
    12301 tgaacgtgga atcgtggata tcgagaccct tcaaaccact caaaatgatt tgatcgaaac
    12361 aatccaagag acattgcgta tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt
    12421 agagcttgga cacatggaag aagatctgaa acaaaaactg ttggatttaa cccaatagat
    12481 cacctttcgc ttgttactaa aacaaacgcc caacaacagt ttattacaac tgttgatggg
    12541 cgtttttctt atctttcttc taacttctcc attggagtct atttctcatt catcttgctg
    12601 tgtccatagc catagccaaa ataaatgatc agtccaataa ccattacgat accgaacacg
    12661 atccatgtca cagtttgcag acgaagcatc aatacaatac aagtcaagat ggagacgatt
    12721 ggcaatagcg gaacgaatgg cactttgaat tctccagctt ttggtttgcc aaacattttt
    12781 ctcaacttca gtaagcctag actaacaagc atcaaatagg cgagcgccat gatattcgtt
    12841 agttccgcta atacattcaa tgggaaaaat ccggcaaaga atacagctgc cacaccagcg
    12901 gcataagtcg cttttttcgg tgtacgatgc ttttgatcga tttctgtcat aaacttagga
    12961 agtaagccat ccttgctgac agcaaacagt aaacgagcaa gtgaatacat catcgagatc
    13021 gttaccgtca gtagcgttaa aattgcccct acagcgatca ccgcgcccac tgttccatgt
    13081 cctacatacc tcattgcaaa agccactggg tcattgactc ctagttcagt atacggaacg
    13141 attcctgtca agatcagcgt cacgatgatg taaaggacag tagcaatcag tatcgaacca
    13201 atgattcctc ttggcacatc tttttgcggg tttttgactt cctcggctgc cattgcaacg
    13261 gcatcaaatc ccaagaaagc aaaaaacaca agagctgcac catctagcac accggaaaaa
    13321 ccaaaaggca tgaatggctg ccagttatca ggtttaacga aaaatattcc tacaatgata
    13381 aacaacgcga tgataccaaa tttgacatat accatggcat tattcaaacg taatgctttc
    13441 ttggcttcaa gcgacacgat gtaagtgacg aatatcacta cgagcaccgc aatcaagtca
    13501 atgtatgtac cattttccgg attgtacccg ccagttaatg cttgtggaag ttcggcccct
    13561 aaacttttca aaaaaccttg catatagcca gaccatccgg aagcaacaga ggaaaccgct
    13621 agtaaaaatt ccccgatcaa caaccaccct gtcagccagg cagcaaattc accaaataca
    13681 acatacagat aggcatatgg cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa
    13741 cttagtccag acaaaatgac aacgattgca gctaagacaa atgaaagtga cagtgctgga
    13801 ccagcatttt gattggccgc aacccctgtc accacaaata ttcccgtacc gatgatcgca
    13861 ccaattccaa gcatgatcaa atcagacgtt tttagttctt ttttcatctc actcgtatga
    13921 ttgagattga tctctttctt acgaaacaaa tccattgaag ttcctcccca ttttaggttg
    13981 gaaaacagcg attaaactgt tttcatatac cctaaaagga taccacctaa aatgactaaa
    14041 aggcaaccaa caataacata aatgagctct tttttcgttt ttctttcgcc aaggatgaaa
    14101 atcccaccta aagtagagat aatgatcccc atttgtgaaa gtgagaagcc aacagctaaa
    14161 ccaagtgatt tgactgttag aagcatgcag atgtttccta agccccataa taatccacag
    14221 atcatgtttt tccacacaaa ctgatcgact ttaactttac ggaaagcaaa gaagcttgcg
    14281 cctaagatca ttccgatact ttgaggtaag ataattgaca tcgcatccaa attcgcccag
    14341 ttgacgatga tggtatatac accataacca atcgtagaga agatcaatgc tctaaaaccc
    14401 ttaccaaaat caagcatttt attatctgta tctatttttc cttcgtcatc ttgtcgggca
    14461 gtcaaatacg caccgcttac aagaagagct aaagcaataa taccgtatac ataatctttc
    14521 gtttgtgtcc attcatggaa gaaaacggct cccgcaatcg tattcaaaat taattgaaaa
    14581 ccagtagaca atggtagccc tacagaaaca cccatgtact tcattccatg gaactgtcca
    14641 ttttgtccaa cactccaagc taagccggat aaaaatccga tcaagaaaat ccaaggggtg
    14701 attacaggac taacaacgaa aaagacaact aaagaaaaca gaaatgcacc gatcgtcatt
    14761 cctagtgttt gttgattggc atcgccacca attttaccac ttactaaacc aatactaccc
    14821 catgccaaca tagggactaa tgctattaaa atttccataa ccaatctcct tttatttcac
    14881 atgataagaa acttatcagc gaaaacgtta acaacacaag cacttttttt cgtttctaac
    14941 gattttctaa cgtttttcat tataaaaatt taagattggg aaaaataaag tcattgttgt
    15001 cccttttcct tcagttgatt cagccgtcaa atggatacct aatttttcgg ccagattttt
    15061 tgctaaataa agtcctaatc ctgttgaatg tgttttgcta agtcgaccat ttctcccagt
    15121 aaatccctta tcaaagatcc gtttcaagtc ttccgctggg ataccgatcc ctgaatcttt
    15181 aactgaaagg ctgactcctt gcgcttcctt ttctatgctc acaatgatgt ttccgtgatc
    15241 cggcgtatat ttgatggcat tactaagcaa ttggttgaag ataaaagcga tccatttagc
    15301 atcggtcaaa acagtatgat cctcaccaac gatctcgtag cgaatatttt tttggataaa
    15361 ataatttgcc tgactgcgta tcacaggctg aatgattcct tttagtccat attcttggat
    15421 gaggtagtcc cgcgagaaac tgtcgagtct tgcataatac aaaacttgtt ctacgtattc
    15481 atcgattttt gataattcat tttctagtaa tgtatacttt tgatcgtcaa tatcgaactc
    15541 gatcgaacga actagcaatt tagaagcgga aagtggcact ttgatttcat ggacccatga
    15601 gtcaatataa tctttttggt cttgttgatt actgattgct gattgcatta cttcttggtg
    15661 ttcacggatc agctgattga tatattcttc taccagtttt tcttcttctg tcctcccacc
    15721 actcaaataa ttttgcaaag gggaagtttt ttgtatgtcg gctaattttc cccaccatct
    15781 tctcttgctc aagtaaccac ccagcagata aaaaatcaga aagacgccct caatcaaagc
    15841 taaataagca attgtgctca aatcaacttt gatgtttggc gccagccaca tcacaaagat
    15901 cgtcaatgca ataaaaaaca gccatcccaa gatcaatgtc cactgatctt tcaaatattt
    15961 aaaaaaattc atcttaaccg ctcatccttt cagggaacga tatatccctg tcctactttt
    16021 gtttcgatat atccttggat acctgcctgt tcgatttttc ggcgtaaccg attgatgttg
    16081 actgtcaatg tattgtcatc tacaaatcgt tcgtcatccc acagtgctcg cagcaatttt
    16141 tcccgactta agatctttcc attgtgcttc atcaaaataa ataatagacg atattcattt
    16201 ttgctcaaat caatgatctc tccgttgatt tccatacttc cgttgtcaac attcaacgta
    16261 atgccattgt ggctcatcat ttcactagat aattctgtgt agttgtaaga acggcgaagt
    16321 aatgcattga tttttgcaat cagtacatcg atttggaaat gttttgtcac aaaatcatct
    16381 gcccccatgt tcatagccat gatcatatcc atattcgtat tcctgctgga gataaaaatg
    16441 atcggtactt tcgatacttc acgtattttt tgacaccaat aatagccatc caaaacggga
    16501 agattaatat ctagtaatac aagctgtggt tcttctcttt caaagtctga gaacacttga
    16561 ttgaaatcgg tcgttccaaa ggtttcaaat tgccatttct caagttcctc catgatcaat
    16621 tgtcgaatga tctcttcgtc ttcgactacc atgatttttg ccataaaaaa tccctccatc
    16681 tttcttcttg ctactacaat atcaaagaag cccttgtgaa acaagttggc ttacctggaa
    16741 aatgtcattt tagcaaacgt taaaaaaaac tggaaatatg cgatttaatc gcgtttgtct
    16801 cttccaagca gcttaaattt attatgttat tcttaataat tggtctatta tctgttattt
    16861 ttctttcgtt gtttttgtct tttaaatttt cgataacacc aataaccgaa aagcgttagc
    16921 actagcaata tacctagaag gagtataagt aatcgataaa gatggtattt ttttgtctgt
    16981 tctatctttg aagagacttc ttctgtaaag ggaactcgtc ttcctgtcac tagtaatcgg
    17041 tgagtgttca cggtgtaagg tgtacaagtg ataagtgtga ctagatcctg cttttctcta
    17101 attgctaatg tatttgtttt tgttggaagg acaacaattt tctcaacgac ttcataagcg
    17161 agcatccgac catacacttc aagatagaat cggtcatctt gctccatttc ttcaagatcc
    17221 gtaaagagtt ttttctcagc aataccgcta tgggcggtaa tcacggaatg tgtactctct
    17281 cctccagttg gaaaagaggt gccttgcagg actgtcgctc ctctatctaa caaaagatca
    17341 ttcgtttcat caaataaagg caagctaaca ttgattttcg gaatatagat ggctccgatc
    17401 atatgttgtt cgtaatattc ttttcctgga ttttggacat ttctaaccgc ttgctcaaaa
    17461 ggatctttga cttgtcccat tcctgggatt actgatgtat gagcaagcgt ctgatttttc
    17521 ttttcatgca ctgctaattg ttgctcatgt ttttcttctt tatcgcgttc aagctgcttt
    17581 tgataattct cgatccttct ttgatccaaa taattgctga gagcatctgc taaaaatgga
    17641 tatgtaaaaa ctgcagctcc ggcaagaaac atcactgcca taaataattg taataaaaga
    17701 ctgatttggg tttgaggtct attacctatt ttctttttca ttttcttcac cttcttattt
    17761 aaaaaaactg cagcaacctt tataaaaccg attgctgcag cttatttttg ttgcttatac
    17821 ttcagacttc attttagatt tacggtacca gctgtacgct ccaatcatca aacttattcc
    17881 gattagtaag aatgccaaga gcccgttccc accagtagca ggaagtgacc cgcgtttcat
    17941 attcggtact ctttctggcc ctggattttc tactcctacg atctgaattt cttcattata
    18001 gcttccataa gagatgataa attcagtaaa ggctgcctca cctgttggaa gtacataacc
    18061 ttctggcgcc atggtctctt tcatttgata ggttccataa tctaaccctt gaacctcaaa
    18121 gtatccattt ccatctgcga caaattctgt tgcttgtccg cttgttgccc actcaattct
    18181 atctgctcct gattcataaa cagctaaagg cgactcccct ttataaaata ttgcgtattc
    18241 agaacggtct tcattccaca attcgaatct tgcatcttta agcaattcac tgcttgatgc
    18301 atcatatttt tcaaactttc ttccacctgt tgtgactttc tctggcggct ctactccttc
    18361 ttcataatca aattcatcat ttcgtccgat ttcaataacc gctgtgttgt tatggaaatc
    18421 atccgcttct agttctgtgg aaaccttcaa attatacgtg atcgttaaat ctcctccacg
    18481 tgcatctgct aatctaccta aagtatctac tttggctact ccattttctt ctgtcaaaat
    18541 cgtcacgaca aacccgttgc ttactacttc tactatataa tcttgtcctt ttgttaaaat
    18601 cgtctcgccg gctcgcactt ctatttcctg atcataataa gttagaccaa ctgtaggcgt
    18661 atctgtgatt ttgtaatttt tgataacagc gcttccgttt tcaaggacag aatcgatgaa
    18721 ataaggaata ggtgctgtga tcgtgtaaga agccgttttt cctctttcta gatcaatata
    18781 agaaatcgtt tcttgttctc cagcttcatt aataatcgtt acttgtctaa ggtcgctttc
    18841 ctctacattc atctgtttat ctgcttctct gatttcgttt ttcggataca aatgcacatc
    18901 tgtattgtat gtgtctttcc atgtagaacc atcccacacg ccatccacca tatcaggcat
    18961 catcactggc atcataacaa ccattggaga cgcgattctg gaaatatgag ctggcgaata
    19021 agtttctaag aaaagataga ctttatctct agaagaaggt gcttcttccg tcacttggac
    19081 ggtagagaat accgcttctc cagcttcatt cgttgtttgt ttgtcaatac ttgtcagtcg
    19141 atattttaaa aaccaacttt ctgtgtgccg tttttgaatc cattcaatcg cttcggcaga
    19201 attaagaccg tcttctcgtt ccggtgttaa aggattatct cgaatcaaat cgtaatatac
    19261 ttccgttacg tcaaacaaat caaaatcgac tcctggaata ttttcttcac caaaatccgt
    19321 agaaaccaat ccgctattcg gtcgttcttc cggaacagat gagaaacctt ttttgtgtaa
    19381 tgtaatcgtc acttcacttg gtttttgtgc gacttcttcg gctttgacta gttttccgtt
    19441 cccgaaaatc cctattcccg cagtaacgag taacagtaca ctcattcccg tgacaatcat
    19501 ttttttattc atttttttct ccccgttctt tttttattaa taaaacagca cctaacaata
    19561 aaagaattcc tagtaaagaa atcaacgttc ttgcttcatt cgtttgcggg aatacggacc
    19621 attttcctga ttgacctgga ttgcttggac cactcggctc tttcggtttg tccgttgttt
    19681 cttttacggt ctcgttataa acacgtggtg ttgcggattc ataggcagag actggaactt
    19741 tcccggattc taattcatcc atctcctgtc cgttaactac tacatattgt ggttggcctt
    19801 ttgcatccac ccaaaattca gggatgatga cctcgattct cttacttgct tcatccatct
    19861 cgtaaccttc cacacctttt aattcttcaa aataataggt gccagaaggc aagaaacgtt
    19921 gtcctgtcgt aacaaggccc tctttatccg aaatgaattt gcttacattt ttgtcattca
    19981 aaggatcatt gtctgctggt ttgatccatt gatttttcaa gtcattagca ggagccatat
    20041 ctaaataata tttcccccct tctatcatct gatatagagc aaaaacagca ccttctaatg
    20101 gttttccttt ttcagttgtt cctctttgac gtccatactt gaagaagtat ggatctctta
    20161 gataaccgac atttttagga tagatatgaa tctcttgcag ttcctcgctt gattctgtcg
    20221 ggtgataaat tggcaaaata gcagcaattg ggatagctgt ctgatcgata tcaacatcca
    20281 atccaacttc tggatcaatc gattcttcga aaatcaaata aactgcatcc cgatcttggt
    20341 ttttcttagg tgctttgata cgtgcaatcc cgtctcctaa tccgccaacc gtatctttcc
    20401 cagttgttac cgaagtccct atttggacaa gcctttcttc tgtgattagc tgacggatat
    20461 tgtttctgtt cgtttcagcg atttgctgga cgaattcttt aggagtcatg ttttcagcag
    20521 cttttttttc atgataataa tcggtcatgt cgtaaatggc aaatgtcgct ccatttagtg
    20581 gcaccgtctg tgaaatgata tctgctgttt cccctgcttc ctcagagata gccaagccat
    20641 cattttcatg gtattcaaat tgatcgttat aatcgatatc tcgaatcatc cgtttatgca
    20701 acacgaattc cacgacatct tcccctgatt cttctgccga catcgcctgt ggaagaagga
    20761 ctgctgcaag cggtagtaaa acaagtaatt gaagcatcaa acacaacagc tttttcatta
    20821 ccttgctccc tcctttttag attgtctgac aaacaaacca taggctgctc ctgcaagcgc
    20881 catgaagaat gcagcaagca ggatatagaa taatcgtccg gatcctcctg tagcaggcaa
    20941 cggattttca ggatcgttcc cgacactaag ctgtaatctg attcgatttg tctccaaaag
    21001 ttcatattct aactcttgct gtagatcagg cgtctctgct tcttcgtttg gataatacga
    21061 aatctcaact tttagtgtac ccgcttcttt tcctggaact acccgatcat tttctatgtc
    21121 aggttcttct cgataactaa tttctaaaat aaagtaacct ggaagttttc tatagctgtc
    21181 aggtgacttc acttccttga tccgatagac cccttccaac aagtctgaag cattgaatag
    21241 ggaagaatcg tttggatctg gtgccaagcc tccctcgatg atttcatact ctcctgcact
    21301 attttgtttc tcaattgaaa attctgcgcc ttcaagacgt cggctattca tactgtcaat
    21361 tttcaaaagt tccaaatcga ttggcgctag ttcatcttct aaggttactt gtagtacacc
    21421 atcgatgatt tcatagcctt ccggcaaggg agcagtttcg tcttcgatga ccgtatcttc
    21481 aatgataaac ttaccttctt cattcagtgc aaactggaac acttgatcag atagccgata
    21541 cccatttggt gcgacagatt ccgagacctc atattcacca atagacagat gcagaggttg
    21601 gtcgtaattt aaatcgacat acttttctac tcctacggca cgagaataga agtatccgat
    21661 cccaggcaaa cgtttcgatt cttctggttc taacggatta ccagtgtatg ctcgatacag
    21721 cgggaaggaa gaatccccca ctttcctgat atcgaaacct gcaccatcta atgtatggcc
    21781 ttcatggtct aattttctaa ctcggatctc ccgataatca aacgtattgt aagcagtgaa
    21841 tgctacccat aaacgatcct cttcatctgt tgtatacgtg agatattgcc aattttcttc
    21901 acctgtttgc ttgagttgga tagtccctat agcattatcg taaagtcgca agatccatgt
    21961 gtcattttca tcaggttctt tgaaaccttc tgggttctgg ctctcaatga ttttgtattc
    22021 tgcatagttg gtagtttctt cactatcctt ataaacaaat gaatctggct ctccgattgg
    22081 atgaataact ttctcaccat ccacgattac atatttcccg tcttcatcta ctgaatagaa
    22141 cgtgagtctc cctttctctt cctctacttc gttcacacca gaaagccctt tcgctactgg
    22201 atatccgatt tccagtccgt taccatctgt ttcataaagt tcaaactctg cgccattgat
    22261 tggcatatta ctgccttcaa ttagtttatt gatattcatg tggattggct tgaattcatt
    22321 tcgcatttcc agtttgacat acaactcatc atactctgtg ccttctatgt taggctcttg
    22381 tacaacagaa agcttgtgat attctccttc ataataggaa ttcggatctt taccttccgg
    22441 tccgccgact ctcgaaccag atgtatattc atctacaagc tcgtagtgac ctaacatctc
    22501 atcttcgttg gctgcatatt ttttgatttg gaattgatac gtctcatata ctaactgata
    22561 acgtggtggt ccagcgatct ctctgatcca aaatatgtcg ctgactgctc cagtttcatc
    22621 tcctaaaatc ggcatcttcg tttcaaagat cgctattccg tccgtggtag ttttacttgt
    22681 ttcgattggt tcggcttccc cactctctgg attttggtca tataatgcaa attcgactcc
    22741 agccaacggc tcgccatttt gacccttctt gctgacttgg aaattacggc ctttatgttc
    22801 gtaacgaatg gaagaagatg ggaaatacat ccgtccagta gtgatatcat tctcataatc
    22861 ctccaggttc tgaatatagg ccatattgtt cactgtgtaa aaatttccgt cttgagctcc
    22921 cgcatcaagt tttacttggt acttgatcct gaccatttcc ccttcaccta aagaaatcgg
    22981 agaaaattta taagcttggt tttcccgtcc gacatacgtt gtcggagtcg ccactacttc
    23041 tggtggggct tgtccatgac gttcttcatc ccatatttct ggctcttggt acttcatgcc
    23101 gttttctcct ggatctagat ggtaaaactg aatatctgcc gatctgccag ctccaaatgg
    23161 tgagacatta aaaccagttt catcataaag tgtggcttgc tggatcgttc tcttgaaggt
    23221 agaagagata tcctccaagg ctgtaacaat gtcattcttg ttgttcgcat tgtaatatcg
    23281 cggtgttacg ccatcttctt ctaaatcagc aatatttttc aatacatttc gtccaatagc
    23341 atctagtcgt tgtctcccgc taacgtttcc atcgattcct aaaccaatgg agaagatact
    23401 tggcgggttc tcttgatact tgtttttcag ccaatgatga taagcaactg tgttgatcgc
    23461 agatgacttt tcttgcgaag taggactgcc attcgtattg ttattagaag aaccatcacc
    23521 aacccagtga gtcgctttat tatctccttc ttttacctct ccataacgat agtgtacatt
    23581 tacttcatct tctggacggt taaagtcaga tgcataggta actggatgag taaaccctgc
    23641 attagaagta ttcccagaaa actcctcaaa atttctgaac aaaacgtcat tatcttcagc
    23701 aatcttatct tccatcacag tccagttgct ccaagctcca ttagtgctcg tacgataact
    23761 tgactgaatc ggtgcataac tgaaggtcgg tgtcccatct cccacaacga ttaggatttt
    23821 ttctggattt ctattttctc cgccattgtc tttatacaat gtttcatagc catttttaag
    23881 ccctagtgta agcggagttc ctccgattgg tgtacgattt aatgctgtat tctcacttcc
    23941 cgtcaaacga ttgatatcat ttgtcaatgg aaagatgtcc gaactgattt gctcatggtt
    24001 attgatagct gtactattat ggtaaaaatt caccataccg attcgaatat ccagactcgg
    24061 gtcagagagc agatctttac tcatttcgtt tactgcctca atcaacgcag catttttagt
    24121 ttgactattg gtcccagcgg ttaattcact catactcgct gacttatcta gaacaaaaac
    24181 gatatcgatt ggactgattt cctttttctc gcccccgatg acatctagat agacattgta
    24241 aagggttgtt gcatcttcag gatgtagatc agatgctgtg ctgcttggca gaacaggttc
    24301 tacccatttt ttgatgtacg ccccgttata atccaagtaa ctgttttcaa aattccgttc
    24361 atttccccaa agctgaacgg tttgagctgg aacgtttcta tcttcttcgc ctgcagttga
    24421 tccattatta gtagcatagt cgtaatttcg ccagttcgct cccccgtttg tataatcact
    24481 atcatcgatc aagttctctc cgtcatatgg cgagcctttg tctccttgat agcgttcatt
    24541 gctttgtgtc caatttccta acacttttgt atagtcattc gttgtaaatt tcgggtagcg
    24601 atgctgttcg ttttcaactt ctccgctagg atagatatat tcataggggt cttgcgtagc
    24661 tgttggccca agacgctcca aaataccgtt gaataactgg cgttgaatgc gctggagtgc
    24721 ttgaccgctg ggtacgattg gttcgtccag cttttccatc tcattttctt cgctacgttg
    24781 aatttcagcg gaatcttctg tatcagtttc attttcttgt aattcttggt tatctattct
    24841 ttcatcaacc accacttgaa gaggtagttc cccttcgttc acatcgggga tcttttctgt
    24901 ttcaaaaatc aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc
    24961 ttgaccatcg atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc
    25021 tgttccacga acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg
    25081 tattctacct tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc
    25141 ttctttttca atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc
    25201 ctcctttgtt tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg
    25261 gaaatatggt ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc
    25321 tttcacatcc ttatactcct ttcaaaaata tttgtcttct ttcacaacaa tcaacaaaa
//
LOCUS       DADJYG010000022        25087 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.022, whole genome shotgun sequence.
ACCESSION   DADJYG010000022 DADJYG010000000
VERSION     DADJYG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.022"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="JZ382_000786"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="JZ382_000786"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY2426990.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="JZ382_000787"
     CDS             complement(1172..1867)
                     /locus_tag="JZ382_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAY2426991.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="JZ382_000788"
     CDS             complement(1880..2596)
                     /locus_tag="JZ382_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAY2426992.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="JZ382_000789"
     CDS             2926..3534
                     /locus_tag="JZ382_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAY2426993.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="JZ382_000790"
     CDS             3859..4182
                     /locus_tag="JZ382_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAY2426994.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="JZ382_000791"
     CDS             4364..5734
                     /locus_tag="JZ382_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAY2426995.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="JZ382_000792"
     CDS             complement(5864..7165)
                     /locus_tag="JZ382_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY2426996.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="JZ382_000793"
     CDS             complement(7519..9510)
                     /locus_tag="JZ382_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAY2426997.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="JZ382_000794"
     CDS             complement(9654..9920)
                     /locus_tag="JZ382_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY2426998.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="JZ382_000795"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="JZ382_000795"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY2426999.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="JZ382_000796"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="JZ382_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY2427000.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="JZ382_000797"
     CDS             13264..13827
                     /locus_tag="JZ382_000797"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY2427001.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="JZ382_000798"
     CDS             complement(14080..14601)
                     /locus_tag="JZ382_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAY2427002.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="JZ382_000799"
     CDS             complement(14604..15446)
                     /locus_tag="JZ382_000799"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAY2427003.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="JZ382_000800"
     CDS             complement(15439..16164)
                     /locus_tag="JZ382_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY2427004.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="JZ382_000801"
     CDS             16340..16636
                     /locus_tag="JZ382_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427005.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="JZ382_000802"
     CDS             17204..18796
                     /locus_tag="JZ382_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2427006.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="JZ382_000803"
     CDS             18809..19549
                     /locus_tag="JZ382_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY2427007.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="JZ382_000804"
     CDS             19717..21066
                     /locus_tag="JZ382_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2427008.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="JZ382_000805"
     CDS             complement(21194..21784)
                     /locus_tag="JZ382_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAY2427009.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="JZ382_000806"
     CDS             22020..23099
                     /locus_tag="JZ382_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2427010.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23320..24672
                     /gene="brnQ"
                     /locus_tag="JZ382_000807"
     CDS             23320..24672
                     /gene="brnQ"
                     /locus_tag="JZ382_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAY2427011.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7174 a         5135 c         4886 g         7892 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    23281 aaatttacga cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt
    23341 aaagactatc tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt
    23401 atctttcccg ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt
    23461 ttcttggtta ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa
    23521 agctcaggtg tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc
    23581 atcttgcttt acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct
    23641 ttcgagattg gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt
    23701 ttcagtattg tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta
    23761 gattatgttg gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc
    23821 cttgcttttt tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa
    23881 gagcatgctt ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct
    23941 ttagcatttg ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct
    24001 gaaatcgcta aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt
    24061 tatactttgc tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat
    24121 ggcgggatca cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta
    24181 ttagcattta tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt
    24241 gcagaaacat tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct
    24301 agtatccttc cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg
    24361 cctgttttga tgttcatcta tccattagca atgacactga ttttactagt gttagttggt
    24421 cctttgttta agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct
    24481 tcgatttttg atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa
    24541 aatcttttat atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc
    24601 gtaccagcag ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa
    24661 gtagcagatt gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa
    24721 ttttgaaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactgact
    24781 tttgaacacc gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat
    24841 attttgtgaa aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg
    24901 cttttgaaca ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg
    24961 gcaaagtagc tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat
    25021 caaacaacat ttcttttata atatagattt tagacatata tatgatataa aaatagtttt
    25081 cgcaaaa
//
LOCUS       DADJYG010000023        24603 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.023, whole genome shotgun sequence.
ACCESSION   DADJYG010000023 DADJYG010000000
VERSION     DADJYG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.023"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            10..738
                     /locus_tag="JZ382_000808"
     CDS             10..738
                     /locus_tag="JZ382_000808"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY2427012.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            738..992
                     /gene="purS"
                     /locus_tag="JZ382_000809"
     CDS             738..992
                     /gene="purS"
                     /locus_tag="JZ382_000809"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAY2427013.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            994..1665
                     /gene="purQ"
                     /locus_tag="JZ382_000810"
     CDS             994..1665
                     /gene="purQ"
                     /locus_tag="JZ382_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAY2427014.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1666..3888
                     /gene="purL"
                     /locus_tag="JZ382_000811"
     CDS             1666..3888
                     /gene="purL"
                     /locus_tag="JZ382_000811"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAY2427015.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3873..5312
                     /locus_tag="JZ382_000812"
     CDS             3873..5312
                     /locus_tag="JZ382_000812"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY2427016.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5344..6387
                     /locus_tag="JZ382_000813"
     CDS             5344..6387
                     /locus_tag="JZ382_000813"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY2427017.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6384..6962
                     /gene="purN"
                     /locus_tag="JZ382_000814"
     CDS             6384..6962
                     /gene="purN"
                     /locus_tag="JZ382_000814"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY2427018.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6959..8494
                     /gene="purH"
                     /locus_tag="JZ382_000815"
     CDS             6959..8494
                     /gene="purH"
                     /locus_tag="JZ382_000815"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY2427019.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8510..9784
                     /gene="purD"
                     /locus_tag="JZ382_000816"
     CDS             8510..9784
                     /gene="purD"
                     /locus_tag="JZ382_000816"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY2427020.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9906..10820
                     /locus_tag="JZ382_000817"
     CDS             9906..10820
                     /locus_tag="JZ382_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY2427021.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10953..12518)
                     /locus_tag="JZ382_000818"
     CDS             complement(10953..12518)
                     /locus_tag="JZ382_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY2427022.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12511..13962)
                     /locus_tag="JZ382_000819"
     CDS             complement(12511..13962)
                     /locus_tag="JZ382_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2427023.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13976..14992)
                     /locus_tag="JZ382_000820"
     CDS             complement(13976..14992)
                     /locus_tag="JZ382_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2427024.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15212..16300
                     /locus_tag="JZ382_000821"
     CDS             15212..16300
                     /locus_tag="JZ382_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY2427025.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16354..17880
                     /locus_tag="JZ382_000822"
     CDS             16354..17880
                     /locus_tag="JZ382_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY2427026.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17882..19087
                     /locus_tag="JZ382_000823"
     CDS             17882..19087
                     /locus_tag="JZ382_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427027.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19344..20321
                     /gene="guaC"
                     /locus_tag="JZ382_000824"
     CDS             19344..20321
                     /gene="guaC"
                     /locus_tag="JZ382_000824"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY2427028.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20585..22009
                     /locus_tag="JZ382_000825"
     CDS             20585..22009
                     /locus_tag="JZ382_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAY2427029.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22470..23579
                     /gene="pdhA"
                     /locus_tag="JZ382_000826"
     CDS             22470..23579
                     /gene="pdhA"
                     /locus_tag="JZ382_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAY2427030.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23582..24559
                     /locus_tag="JZ382_000827"
     CDS             23582..24559
                     /locus_tag="JZ382_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAY2427031.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         7952 a         4198 c         5568 g         6885 t
ORIGIN      
        1 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
       61 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      121 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      181 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      241 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
      301 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
      361 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
      421 tatttcttac aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct
      481 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
      541 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
      601 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
      661 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
      721 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
      781 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
      841 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
      901 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
      961 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1021 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1081 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1141 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1201 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1261 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     1321 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     1381 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     1441 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     1501 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     1561 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     1621 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     1681 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     1741 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     1801 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     1861 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     1921 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     1981 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2041 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2101 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2161 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2221 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     2281 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     2341 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     2401 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     2461 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     2521 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     2581 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     2641 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     2701 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     2761 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     2821 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca
     2881 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     2941 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3001 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3061 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3121 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3181 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3241 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     3301 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     3361 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     3421 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     3481 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     3541 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     3601 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     3661 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     3721 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     3781 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     3841 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     3901 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     3961 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4021 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4081 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4141 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4201 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4261 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     4321 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     4381 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     4441 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     4501 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     4561 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     4621 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     4681 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     4741 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     4801 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     4861 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     4921 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     4981 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5041 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5101 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5161 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5221 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     5281 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     5341 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     5401 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     5461 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     5521 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     5581 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     5641 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     5701 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     5761 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     5821 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     5881 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     5941 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6001 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6061 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6121 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6181 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6241 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     6301 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     6361 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     6421 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     6481 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     6541 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     6601 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     6661 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     6721 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     6781 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     6841 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     6901 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     6961 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7021 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7081 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7141 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7201 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7261 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     7321 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     7381 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     7441 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     7501 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     7561 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     7621 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     7681 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     7741 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     7801 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     7861 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     7921 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     7981 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8041 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8101 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8161 attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8221 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     8281 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     8341 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     8401 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     8461 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     8521 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     8581 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     8641 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     8701 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     8761 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     8821 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     8881 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     8941 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9001 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9061 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9121 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9181 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9241 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
     9301 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
     9361 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
     9421 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
     9481 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
     9541 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
     9601 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
     9661 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
     9721 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
     9781 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
     9841 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
     9901 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
     9961 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10021 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10081 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10141 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10201 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10261 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    10321 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    10381 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    10441 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    10501 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    10561 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    10621 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    10681 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    10741 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    10801 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    10861 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    10921 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    10981 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11041 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11101 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11161 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11221 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    11281 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    11341 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    11401 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    11461 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    11521 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    11581 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    11641 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    11701 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    11761 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    11821 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    11881 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    11941 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12001 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12061 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12121 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12181 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12241 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    12301 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    12361 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    12421 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    12481 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    12541 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    12601 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    12661 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    12721 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    12781 tttgcatgcg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    12841 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    12901 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    12961 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13021 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13081 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13141 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13201 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13261 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    13321 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    13381 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    13441 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    13501 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    13561 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    13621 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    13681 gatctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    13741 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    13801 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    13861 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    13921 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    13981 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14041 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14101 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14161 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14221 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    14281 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    14341 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    14401 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    14461 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    14521 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    14581 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    14641 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    14701 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    14761 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    14821 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    14881 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    14941 tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15001 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15061 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15121 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15181 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15241 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    15301 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    15361 cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca
    15421 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    15481 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    15541 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    15601 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    15661 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    15721 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    15781 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    15841 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    15901 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    15961 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16021 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16081 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16141 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16201 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16261 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    16321 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    16381 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    16441 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    16501 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    16561 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    16621 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    16681 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    16741 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    16801 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    16861 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    16921 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    16981 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17041 atcagcgaag agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct
    17101 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17161 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17221 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    17281 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    17341 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    17401 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    17461 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    17521 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    17581 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    17641 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    17701 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    17761 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    17821 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    17881 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    17941 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18001 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18061 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18121 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18181 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18241 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    18301 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    18361 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    18421 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    18481 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    18541 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    18601 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    18661 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    18721 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    18781 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    18841 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    18901 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    18961 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19021 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19081 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19141 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19201 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19261 attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat
    19321 gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc
    19381 ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac
    19441 atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg
    19501 cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc
    19561 gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg
    19621 tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt
    19681 atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata
    19741 ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg
    19801 tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag
    19861 tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta
    19921 gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg
    19981 gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg
    20041 ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat
    20101 actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa
    20161 tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc
    20221 aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg
    20281 tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag
    20341 gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct
    20401 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
    20461 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
    20521 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
    20581 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
    20641 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
    20701 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
    20761 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
    20821 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
    20881 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
    20941 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
    21001 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
    21061 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
    21121 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
    21181 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
    21241 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
    21301 aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt
    21361 tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg
    21421 tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa
    21481 ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc
    21541 gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt
    21601 tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca
    21661 aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa
    21721 aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa
    21781 gcttcattca attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga
    21841 ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt
    21901 tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc
    21961 gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt
    22021 aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg
    22081 aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat
    22141 gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa
    22201 cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt
    22261 ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca
    22321 tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga
    22381 agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt
    22441 cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc
    22501 aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg
    22561 gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa
    22621 tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag
    22681 gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt
    22741 acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa
    22801 aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt
    22861 cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac
    22921 aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt
    22981 atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg
    23041 cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc
    23101 gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg
    23161 gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg
    23221 aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac
    23281 cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt
    23341 ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt
    23401 ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg
    23461 ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag
    23521 tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat
    23581 catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa
    23641 tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc
    23701 aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga
    23761 atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga
    23821 aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg
    23881 tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg
    23941 tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc
    24001 tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc
    24061 ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt
    24121 ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg
    24181 tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc
    24241 agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc
    24301 gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt
    24361 ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg
    24421 cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc
    24481 atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa
    24541 agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa
    24601 att
//
LOCUS       DADJYG010000024        24559 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.024, whole genome shotgun sequence.
ACCESSION   DADJYG010000024 DADJYG010000000
VERSION     DADJYG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.024"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            67..444
                     /locus_tag="JZ382_000828"
     CDS             67..444
                     /locus_tag="JZ382_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427032.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(624..1919)
                     /gene="purB"
                     /locus_tag="JZ382_000829"
     CDS             complement(624..1919)
                     /gene="purB"
                     /locus_tag="JZ382_000829"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY2427033.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1932..3065)
                     /gene="purK"
                     /locus_tag="JZ382_000830"
     CDS             complement(1932..3065)
                     /gene="purK"
                     /locus_tag="JZ382_000830"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY2427034.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3049..3537)
                     /gene="purE"
                     /locus_tag="JZ382_000831"
     CDS             complement(3049..3537)
                     /gene="purE"
                     /locus_tag="JZ382_000831"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY2427035.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3830..5137)
                     /locus_tag="JZ382_000832"
     CDS             complement(3830..5137)
                     /locus_tag="JZ382_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY2427036.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5140..5721)
                     /locus_tag="JZ382_000833"
     CDS             complement(5140..5721)
                     /locus_tag="JZ382_000833"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY2427037.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5837..5934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6293..7585)
                     /locus_tag="JZ382_000834"
     CDS             complement(6293..7585)
                     /locus_tag="JZ382_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2427038.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7582..8205)
                     /locus_tag="JZ382_000835"
     CDS             complement(7582..8205)
                     /locus_tag="JZ382_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427039.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8208..9044)
                     /locus_tag="JZ382_000836"
     CDS             complement(8208..9044)
                     /locus_tag="JZ382_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427040.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9061..9948)
                     /locus_tag="JZ382_000837"
     CDS             complement(9061..9948)
                     /locus_tag="JZ382_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427041.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9956..11191)
                     /locus_tag="JZ382_000838"
                     /pseudo
     CDS             complement(9956..11191)
                     /locus_tag="JZ382_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11874..12557)
                     /locus_tag="JZ382_000839"
     CDS             complement(11874..12557)
                     /locus_tag="JZ382_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY2427042.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12575..13192)
                     /locus_tag="JZ382_000840"
     CDS             complement(12575..13192)
                     /locus_tag="JZ382_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAY2427043.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13202..14026)
                     /locus_tag="JZ382_000841"
     CDS             complement(13202..14026)
                     /locus_tag="JZ382_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAY2427044.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14019..14579)
                     /locus_tag="JZ382_000842"
     CDS             complement(14019..14579)
                     /locus_tag="JZ382_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427045.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14753..15709
                     /locus_tag="JZ382_000843"
     CDS             14753..15709
                     /locus_tag="JZ382_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2427046.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16014..16970)
                     /locus_tag="JZ382_000844"
     CDS             complement(16014..16970)
                     /locus_tag="JZ382_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAY2427047.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17074..17676
                     /locus_tag="JZ382_000845"
     CDS             17074..17676
                     /locus_tag="JZ382_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY2427048.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17764..19263)
                     /locus_tag="JZ382_000846"
     CDS             complement(17764..19263)
                     /locus_tag="JZ382_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY2427049.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19279..19803)
                     /locus_tag="JZ382_000847"
     CDS             complement(19279..19803)
                     /locus_tag="JZ382_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427050.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20101..20850
                     /locus_tag="JZ382_000848"
     CDS             20101..20850
                     /locus_tag="JZ382_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAY2427051.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20841..21626
                     /locus_tag="JZ382_000849"
     CDS             20841..21626
                     /locus_tag="JZ382_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAY2427052.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21595..22311
                     /locus_tag="JZ382_000850"
     CDS             21595..22311
                     /locus_tag="JZ382_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY2427053.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22413..23432)
                     /locus_tag="JZ382_000851"
     CDS             complement(22413..23432)
                     /locus_tag="JZ382_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAY2427054.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23451..24509)
                     /locus_tag="JZ382_000852"
     CDS             complement(23451..24509)
                     /locus_tag="JZ382_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAY2427055.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7303 a         5266 c         4289 g         7701 t
ORIGIN      
        1 tacttaatga tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac
       61 ttgccgatgg aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa
      121 gaacgttcct gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa
      181 gatactagtt tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc
      241 cgtaaaagga cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt
      301 catgaacaga agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag
      361 atggaatacg cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat
      421 aatagccaag ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa
      481 gaggtagccg cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa
      541 gaagcagaac cgctaacgga tctgcttctt ttttggttat ttttgctatt ctattttaat
      601 tgaattttgc aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg
      661 tttttcaaat gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat
      721 gtgatggttg gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac
      781 gcagtttttg gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat
      841 ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg
      901 ttttcaggaa agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc
      961 aataaaatcg tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt
     1021 tcatgccata atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct
     1081 agaccagcca tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct
     1141 ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt
     1201 atctctgttg caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca
     1261 gcatgtagat cccgagggag aacttgagta gagatttctt gtggacggat ccccaattgt
     1321 ttacatacat actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag
     1381 atttttcctg cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt
     1441 tctgaatacc atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt
     1501 gtccgaccca tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta
     1561 aaacgtttca agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca
     1621 gtatctacga catccgtact tgttagacca taatggaccc attttctttc ttctcccaat
     1681 gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc
     1741 aagatccgat cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg
     1801 atttcaccaa gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg
     1861 tatttgttct cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg
     1921 ttttttctcc tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg
     1981 tcttctgtca agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca
     2041 tagaaatgga attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt
     2101 tctcccaaaa cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc
     2161 caaccacaaa tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa
     2221 taatgtccag aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt
     2281 gttaaaaaca tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg
     2341 gcaatacgct cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca
     2401 tgaagaatat tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga
     2461 ccatttcctg ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca
     2521 caggttcctt ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca
     2581 tactgcccct tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc
     2641 ccgtcaatcg catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg
     2701 atgttattcg tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag
     2761 tctgtccctt gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg
     2821 ttttcgaatt cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg
     2881 tcatcataga cactttcaat gtgccaatct gcaacttgtg cggctggaca atcgctcaca
     2941 ggatccagca cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct
     3001 aattgaccgc caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg
     3061 atcactactt tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc
     3121 caattctaaa tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc
     3181 cgcttttccg attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag
     3241 aagcgaatcc aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt
     3301 tgtttttgat gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat
     3361 tttgattcct ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg
     3421 agcagaaacg accttttttt cataagacac accgaattca tccaatattt gacatgcttc
     3481 tttcattgtt tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt
     3541 ttttcctccc agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa
     3601 atttcgaatg ttaacaaaga aaaacatttt attgttcgtg tttattaaag atgaaaaaaa
     3661 ggaatgtgac aaaagttagg tcacattcct ttttatggat aaacggtgtt caatcagctg
     3721 accctcggta ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc
     3781 tctaacaatc aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga
     3841 aggcttcttc caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga
     3901 tagccgtaat gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca
     3961 tttgactgaa taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa
     4021 caatcaatag atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga
     4081 ctgccaccat cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt
     4141 gtgctaacgc accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat
     4201 agatgggttt tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc
     4261 cagtataagg gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat
     4321 atcccttttt caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat
     4381 atacacctgt tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc
     4441 atacgtcgaa agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat
     4501 ctccaatcgc gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta
     4561 aaccaatcaa taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa
     4621 ttaggagaat cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg
     4681 ttgcattacc tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg
     4741 tgataaccgt tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag
     4801 aaactaacac aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt
     4861 cattggttcc gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag
     4921 gcaagccaat tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca
     4981 taaagatatc aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac
     5041 cgatcaaaag aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta
     5101 aaaccgcagc ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa
     5161 ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat
     5221 ttcttcaagt aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc
     5281 tacttgcgct ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc
     5341 caagaaatcg tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga
     5401 aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc
     5461 catcgtcaaa ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc
     5521 aaataaagct ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc
     5581 tttaaatact tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg
     5641 agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat
     5701 acgttcaact aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac
     5761 cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat
     5821 aagaaaagaa gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc
     5881 tagaaactgt catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg
     5941 taaattcatt ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa
     6001 aatgttgttt caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt
     6061 tacttatttt tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt
     6121 aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga
     6181 caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg
     6241 caataaggca agattttata aactacggga agtaatttcc gtaaatctat tcttattctt
     6301 ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg
     6361 cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat
     6421 cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc
     6481 cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga
     6541 tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact
     6601 tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt
     6661 cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg
     6721 ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat
     6781 atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt
     6841 ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt
     6901 ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata
     6961 ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat
     7021 gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc
     7081 cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac
     7141 tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc
     7201 gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga
     7261 tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat
     7321 catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta
     7381 gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa
     7441 gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac
     7501 aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc
     7561 cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac
     7621 tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca
     7681 ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa
     7741 cagccagata ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat
     7801 atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg
     7861 aatcagatac caagctccaa tctttcccac taataaatac caatcaactg caaaaataat
     7921 gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca
     7981 acataccttt ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa
     8041 aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc
     8101 tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata
     8161 gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga
     8221 ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc
     8281 ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg
     8341 tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc
     8401 aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc
     8461 gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca
     8521 ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa
     8581 atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa
     8641 ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc
     8701 gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg
     8761 taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac
     8821 gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa
     8881 ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc
     8941 gttttaaaag aacctgcgat catccataag accggataaa tcatgactag acctaatagc
     9001 aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat
     9061 tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat
     9121 caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg
     9181 gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat
     9241 aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat
     9301 gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc
     9361 gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat
     9421 cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg
     9481 atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc
     9541 gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact
     9601 aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata
     9661 agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt
     9721 gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc
     9781 gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat
     9841 catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc
     9901 gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg
     9961 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
    10021 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
    10081 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
    10141 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
    10201 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
    10261 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
    10321 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
    10381 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
    10441 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
    10501 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
    10561 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
    10621 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
    10681 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
    10741 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
    10801 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
    10861 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
    10921 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
    10981 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
    11041 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
    11101 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
    11161 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
    11221 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
    11281 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
    11341 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
    11401 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
    11461 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
    11521 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
    11581 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
    11641 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
    11701 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
    11761 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
    11821 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
    11881 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
    11941 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
    12001 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
    12061 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
    12121 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
    12181 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
    12241 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
    12301 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
    12361 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
    12421 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
    12481 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
    12541 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
    12601 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
    12661 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
    12721 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
    12781 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
    12841 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
    12901 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
    12961 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
    13021 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
    13081 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
    13141 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
    13201 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
    13261 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
    13321 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
    13381 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
    13441 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
    13501 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
    13561 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
    13621 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
    13681 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
    13741 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
    13801 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
    13861 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
    13921 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
    13981 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
    14041 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
    14101 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
    14161 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
    14221 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
    14281 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
    14341 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
    14401 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
    14461 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
    14521 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
    14581 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
    14641 ctgctttcat tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt
    14701 ataataatag acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa
    14761 atatcaatgg ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa
    14821 tcaagagacg agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt
    14881 cgctcaccgt tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc
    14941 tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc
    15001 agcattggaa gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga
    15061 actggctgac gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac
    15121 aatcttcaac atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg
    15181 ggctttaaaa atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg
    15241 gttcttcaat ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc
    15301 tttcgctcgt ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt
    15361 tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc
    15421 tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa
    15481 cggttatgtg acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga
    15541 tggtacaaag gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa
    15601 gaatatggta cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga
    15661 tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt
    15721 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta
    15781 tttttcgttt ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag
    15841 aggttgtgag cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct
    15901 catgtttgtt gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag
    15961 aagaggttgt gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt
    16021 tggatttttt gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa
    16081 aactggaaca attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc
    16141 cgatacaata cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct
    16201 gaaaatgttt ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt
    16261 tgctggattc tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc
    16321 atagagaagt aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct
    16381 ctggcatctt cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca
    16441 tcttctttat tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct
    16501 ttttcgatct gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt
    16561 gcaagcaaag caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc
    16621 caagacatgt tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct
    16681 tcggttaatt tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca
    16741 aaaatagtat acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag
    16801 gctgcgattc gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat
    16861 aatgcaatga tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc
    16921 gaaaagaaga cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc
    16981 cttttttatt ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt
    17041 atgatagtaa aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt
    17101 atgcgtacga tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact
    17161 caacgaggaa tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg
    17221 actacaaccg tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta
    17281 gctatcattt atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc
    17341 ttgcttccta ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc
    17401 ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa
    17461 tccgctttgt atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc
    17521 ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat
    17581 ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct
    17641 aagatccaaa aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa
    17701 aatggagaga agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact
    17761 actttattct tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac
    17821 ttggctagta gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt
    17881 tgtcgctttt agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg
    17941 gttcgctcca ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat
    18001 atttcctagc ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact
    18061 caagacagct gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc
    18121 ttctgctttt ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt
    18181 attggcttcc gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac
    18241 ttctttgact ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt
    18301 tttcttcact tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc
    18361 gatttgtttt tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg
    18421 tgctcgggca ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc
    18481 ttgtttgtag gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg
    18541 tcgctgtaat tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc
    18601 ttttagagct tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt
    18661 ccctaaagag tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat
    18721 gatcaacccc atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt
    18781 atcgcgattt tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc
    18841 ttctaatact tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc
    18901 tgctgctgta gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca
    18961 catcaccggt acgccttgtt cggtatacac ttcaggggtg gagacgtcca atttactcga
    19021 taatagactg atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg
    19081 cacaattttg attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact
    19141 tccactgatg atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat
    19201 cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc
    19261 catttaacac acgctccttt aagcttttat catgctttca taaggcatga cataacagat
    19321 tctttccttg atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg
    19381 gctgtaaaaa caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc
    19441 catacctcgt acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc
    19501 agaagacgac atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa
    19561 aaataccaac actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc
    19621 tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag
    19681 catcggatca agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc
    19741 caatcctgca caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat
    19801 catatacacg cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata
    19861 gcttctttta ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt
    19921 tgttaaattt ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg
    19981 tttccttttt ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt
    20041 ttctccacat tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg
    20101 atgaaccgat ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat
    20161 aaactcatcc aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca
    20221 aatgaaatca tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt
    20281 cgatattctc acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg
    20341 aaaaaagtga ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc
    20401 ttccgctatt tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc
    20461 ttaacaaaca acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca
    20521 ttgattattg gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa
    20581 aaagtactgc ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt
    20641 atcgaaaatg aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg
    20701 atcaatacac ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta
    20761 ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa
    20821 cgaccgttcg gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac
    20881 ctctttttag caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag
    20941 taatgaaatt aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc
    21001 aaacccatga attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt
    21061 atgaagaagt cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc
    21121 aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg
    21181 aagccaaaga acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg
    21241 agatggatga ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa
    21301 aagaagccgt gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg
    21361 acacagaagc tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag
    21421 aaatctcaga aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg
    21481 gattgaatct catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac
    21541 aaacaaatgg cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg
    21601 acaaatgtcc tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg
    21661 gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa
    21721 tatttcagac aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt
    21781 ggtatccatt ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc
    21841 actgctgcag atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat
    21901 ttgatcaaac cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc
    21961 tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac
    22021 cacaccactc ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa
    22081 ctgactttaa aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa
    22141 gaactggctc aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta
    22201 gtagaacaag atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc
    22261 tgttatgaat tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa
    22321 ttatcccata caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt
    22381 taaacatttt attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga
    22441 gatacctcat ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc
    22501 gaaatcatat ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact
    22561 ctagggtaca tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg
    22621 aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa
    22681 tgattgcttt tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta
    22741 ttaatcaaga tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg
    22801 ttttcacctg ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct
    22861 tcaagcgagt ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg
    22921 atcactttgg ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt
    22981 cctatgatcc ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga
    23041 aaatctttga aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca
    23101 aaagtcaaag ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc
    23161 ataccaaaat cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa
    23221 acataacgaa tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt
    23281 cccctcaaca atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt
    23341 tcttcataaa gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg
    23401 ttcggacgaa caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat
    23461 aaacctatgc catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca
    23521 aaaatagttg acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata
    23581 gcataggctg ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt
    23641 gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc
    23701 aatgcaggta caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca
    23761 gcttttttga agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa
    23821 cttaaaccag ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc
    23881 aaaaacgcta cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac
    23941 ccttggaaca gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac
    24001 atcgctttga tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa
    24061 tacaaaaaga ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca
    24121 taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca
    24181 cgcaacgttg tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata
    24241 agaataatat aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct
    24301 gtattgatat cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg
    24361 actttcgata gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac
    24421 cctaataata taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa
    24481 gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca
    24541 caataaaaaa agcttttat
//
LOCUS       DADJYG010000025        23826 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.025, whole genome shotgun sequence.
ACCESSION   DADJYG010000025 DADJYG010000000
VERSION     DADJYG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.025"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            23..775
                     /locus_tag="JZ382_000853"
     CDS             23..775
                     /locus_tag="JZ382_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY2427056.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            772..1686
                     /gene="pfkB"
                     /locus_tag="JZ382_000854"
     CDS             772..1686
                     /gene="pfkB"
                     /locus_tag="JZ382_000854"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY2427057.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1704..3635
                     /locus_tag="JZ382_000855"
     CDS             1704..3635
                     /locus_tag="JZ382_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY2427058.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3746..5305)
                     /locus_tag="JZ382_000856"
     CDS             complement(3746..5305)
                     /locus_tag="JZ382_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAY2427059.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5460..7694
                     /gene="pcrA"
                     /locus_tag="JZ382_000857"
     CDS             5460..7694
                     /gene="pcrA"
                     /locus_tag="JZ382_000857"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAY2427060.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7711..9747
                     /gene="ligA"
                     /locus_tag="JZ382_000858"
     CDS             7711..9747
                     /gene="ligA"
                     /locus_tag="JZ382_000858"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY2427061.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9853..10158
                     /gene="gatC"
                     /locus_tag="JZ382_000859"
     CDS             9853..10158
                     /gene="gatC"
                     /locus_tag="JZ382_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAY2427062.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10158..11630
                     /gene="gatA"
                     /locus_tag="JZ382_000860"
     CDS             10158..11630
                     /gene="gatA"
                     /locus_tag="JZ382_000860"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAY2427063.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11627..13057
                     /gene="gatB"
                     /locus_tag="JZ382_000861"
     CDS             11627..13057
                     /gene="gatB"
                     /locus_tag="JZ382_000861"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAY2427064.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13067..14122
                     /locus_tag="JZ382_000862"
     CDS             13067..14122
                     /locus_tag="JZ382_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY2427065.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14233..15606
                     /gene="rlmD"
                     /locus_tag="JZ382_000863"
     CDS             14233..15606
                     /gene="rlmD"
                     /locus_tag="JZ382_000863"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY2427066.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15609..15737
                     /locus_tag="JZ382_000864"
     CDS             15609..15737
                     /locus_tag="JZ382_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY2427067.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16177..16365
                     /locus_tag="JZ382_000865"
     CDS             16177..16365
                     /locus_tag="JZ382_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY2427068.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16443..17456
                     /locus_tag="JZ382_000866"
     CDS             16443..17456
                     /locus_tag="JZ382_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2427069.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17523..18557)
                     /locus_tag="JZ382_000867"
     CDS             complement(17523..18557)
                     /locus_tag="JZ382_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2427070.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            18672..20897
                     /locus_tag="JZ382_000868"
     CDS             18672..20897
                     /locus_tag="JZ382_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY2427071.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            20915..22798
                     /locus_tag="JZ382_000869"
     CDS             20915..22798
                     /locus_tag="JZ382_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAY2427072.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            22779..23753
                     /locus_tag="JZ382_000870"
     CDS             22779..23753
                     /locus_tag="JZ382_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAY2427073.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         7961 a         4365 c         5174 g         6326 t
ORIGIN      
        1 ttgtattgag gtgatcgtga aaatgcttac agaggaacgc agacaaaaaa tcctagactt
       61 gcttgaacag cagaaaatcg tgaaatcaca agatcttgta aaccttttga atgcttctga
      121 atcaacgata agacgtgatt tacaagagct ggagaatagt gggtttctag aaagagttca
      181 tggcggagct aagaaagagc aattattgaa ttttgagcag aatatgagtg aaaaatcact
      241 caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca gctgaaacca ttaatgacga
      301 agatgtcatt tatttagacg ctggctctac tacgttagaa atgattcctt ttctgcaagg
      361 aaaacagatc acagtagtga caaattccgt tcaacatgcg gcaaatttag ttgatttgga
      421 gcttgctacc attattttag gtggaacgat aaagctggca actaatgctg ttttaggatc
      481 taatgcgata gaacaactaa aaaattttca ttttaataaa gcatttatgg gaatgaacgg
      541 tgcccatctt gaacaaggat tcacgactcc ggatccagaa gaggcagctg ttaaacgttt
      601 agccatgaaa aattccgaag agacatttgt attgattgat cataccaaat tcaaccagtt
      661 gtccttcacc tctgttgcac ctttggaagc agccacgatc ataaccgagc gttgtccatt
      721 agaattcgtt aatgaattcc gtgaaaagac aacaattaag gaggcaatac aatgatttac
      781 acagtaacac ttaatccatc aattgattat atcgtacgcg tagatgggtt aaaactaggc
      841 gatttgaacc gaatgaagga agacttcaag ttacctggag gaaaagggat caatgtttcc
      901 cgtattttga aacggatcga ttctcaatct acagcactag ggtttttagg agggttcact
      961 ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc agtctttgtt cacaaccgta
     1021 aaagaagata caagaatcaa tatcaaacta aaatcagaca ctgaaactga gatcaatggc
     1081 ttaggcccat ctatttcaga tgaagaaatc gatgagttga agcaagcatt aggaacagta
     1141 ggacaaggag atatcgtcgt attgtcagga agcgcgccag ctagcctgcg aaaaggattt
     1201 tatcaagaac tgattgatat cattcgagaa aaaggtgcag atttcgtgat tgatacaaca
     1261 ggtgatgacc taaaaaatgc acttgttaag aaaccgttgt tgatcaaacc aaacaatcac
     1321 gaattagctg agctttacaa cacaaccttc caatctgtag aagaaatcat tccgtatggg
     1381 aaaaaattac tagctgaagg ggcacagaat gtgctgatct caatggctgg agacggtgct
     1441 ttgctcttca cggaacaagg aacttataaa acgaatgtat tagttcgccc attgaaaaat
     1501 tcagtaggtg caggggattc aatgattgct ggctttattg gggcttacag ccgtacgaaa
     1561 gatccaatcg aagcctttaa atggggagtt gcttgcggaa gcgcaacagc tttttctgat
     1621 gacttggcag ctcgcgaatt gattgatgag ttgctgccag aagttgaaat cacaaaaatt
     1681 gattagtttc aaggaggata atcatgaata tcaaggattt aatggtcaaa gaagcgatga
     1741 tcatggattt gcaagcgact gataaaaaag gcgcaatcga tgaaatggtc caaaaaatgt
     1801 ttgatgccgg ccggatttcc gatatcgaaa catataaaga aggaattcta gcccgcgaag
     1861 cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc tcacgcgaaa aacagtgcag
     1921 ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg agtagattat gaagccttag
     1981 acggacagcc aacttatctg tttttcatga tcgcagctcc agaaggcgca aacgacacac
     2041 atttgcaagc attagcagca ctatctcgat tgctgattga tgcagatttc gtaggaaaat
     2101 taaaagaagc aaatacacca gaagaagtcc aagacttgtt ccaaacagct gaagagcaaa
     2161 aagaagcaga agaaaaagca gaacaagaaa cacaagctgc agaagcttct gctcatccag
     2221 aaagaaaatt cgttgttgcc gttacagctt gtccaacagg gatcgctcac acttacatgg
     2281 cagaagatgc tttgaagaaa aaagcgaaag aaatgggtgt ggagatcaaa gtcgaaacga
     2341 acggttcaga aggaatcaaa aaccgattga ctgctgaaga catcgcccgt gcagaaggtg
     2401 taatcgttgc agcagacaaa aaagtagaga tgaatcgctt tgacggaaaa gaactgatca
     2461 atcgtcctgt cagtgatggt atccgtaaac cagaagagtt gatcaatcta gcccttagtg
     2521 gtactgcacc agtattccat ggtgatggaa aagaaagcag ttcagaagaa ggaaatgcgg
     2581 atggtactat cggacaaaga atttacaaag atttgatgaa cggcgtatca cacatgttac
     2641 ctttcgttat tggtggagga atcgctattg cgctttcttt catgatcgac caatttattg
     2701 gcgtgccaca tgatcaatta tcaaatctag ggaattacaa tcaagctgct agctggttca
     2761 accaaatcgg acaagctgca tttggtttca tgctgccggt attagcagga tttattgctt
     2821 caagtatcgg tgaccgccct ggtttgatcg ttggatttgc tgctggtgca ttagctaata
     2881 caggcggtgc aggattctta ggtgcactga ttggtggatt cttagctggt tatgtcatcg
     2941 tatttttacg taaattattc aagaacttac caaaatcatt ggatggaatt aaaacgatcc
     3001 tcttttatcc agtgtttggc ttactgatta ctggtttctt gatgttgctg gtcaatgtcc
     3061 ctatgaaagc aatcaatgat gcattgaacg gtttcttgac aagtatgagt ggttcgaatg
     3121 ctgctttact tggtgcatta ttagctggaa tgatggcggc agatttaggc ggtccaatca
     3181 acaaagccgc ttatgtgttt ggtacagcaa ctttagctac gactgttgca actggcggaa
     3241 gtgtcgtgat ggcttctgtt atggctggag gaatggtacc tccattggca attttcgttg
     3301 ctacacgttt gtttaagaat aaattcagca aaacagatca agatgctggt ttgacgaaca
     3361 tcgtgatggg actatcgttt gtcacggaag gatcgattcc atttgcagca gctgacccga
     3421 ttcgtgccat tccaagtttt attattggtt ctgctttaac tggcggatta gtcggagctt
     3481 tcggaatcaa attactggct cctcacggcg ggattttcgt tgtattctta ctgagtcacc
     3541 cggtcatgta tttggtgttt attgctatcg gagcagtcgt atctggtgtg atttatggtt
     3601 tattgagaaa agctcctgca gtagcagaag catagaaata aaaaagaaga ggctgggttt
     3661 tcccagcctc ttcttttttt atagaatcat atagaatcaa gtagtgtaga tgaatagttg
     3721 ttgtaaaata gtttttccat gttcgttaaa atgattgctt caaatttgtg atagaagatt
     3781 tagcctcgat ttcctgcttg attcttggca agtttgcttc atcagtcaag ataaccaaaa
     3841 tatctcctac attcatgatc gtatcaccgt gagtcaagat ttcctgattt cctctgcgta
     3901 tagagattag cagcatattt ttaggccaat tgaagtctcg taccattaat ccgtctaaag
     3961 aactctctgc cgttactgga aaatcaaaag ttgtccttgc accttttagt gaggcggtct
     4021 gagtatttcc aagtagacgt tccaataatg attcgtaaat tggattaccg ccgagaaggt
     4081 cattgacgat ataagcaaca agagcagcca atcctaaagg catcagatga ttaagagagc
     4141 caaccatttc tgtcactaaa ataattgctg tcagcggtgc ttttccaatc gctgtgaaat
     4201 aaccagccat ggcaataacg acaaaatcct tgatgtagat tggatccatc ccgaaatcaa
     4261 ccataatgtt tccataaaag ctaccgatta aagcccccaa tgtcaatatc ggaaggaaaa
     4321 tcccccctgg aagaccccca ccgtaagaaa tcatggaaaa aacgaatcga agaacaaaca
     4381 aaccgataat cataatgatt ggcaaggaag catgtccaaa agctaaaacg atttgattgc
     4441 ctccgcctag agtactaggc cataaataac caattggcaa aatcagcaag aaaggaacga
     4501 acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa gccagcaaga
     4561 cattctgata aataaaacct aagatgccta gaagtactcc taagatcact aaggaaccat
     4621 aatatttcaa cggcaaagaa ggaaggtcac ctagatataa aacaggtctc aagccgaaaa
     4681 agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc caaacaagtg
     4741 gagaaaagga gtgatggatt tcttctaaaa caaagagtaa tcctgcgata ggagcattga
     4801 atgctgctgc aagtccgcta cttgcaccgc tagaaattaa aatcttttct tctgaagatg
     4861 gactccgaaa caattgtcca gctccttgtc caatggatgc gccaagctgt atagaaggtc
     4921 cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg aactttttcc
     4981 ataaaacaga gaaccagttc attgataatt ccccgcgaag ctgtccctct acttgtggaa
     5041 tcccgcttcc cttgatatcc ggttcatttt tgaccagata acccacgaaa aatgctgcaa
     5101 ggatgcagca aatagctagg ccaattaagt aaatcggctg ttcccgactc atttcataga
     5161 tacttcgaac taaatcgctt agcttttcga tagataatcg aaacaggcta acgacgattc
     5221 ctgtcagcag tccaacaaat attcctttta gaataaaata tatctgagtc ttatctaatt
     5281 tcttgatttt tccttttttt tccattcaaa cgcctcgctt tcttttaatt tagcgcgaaa
     5341 tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa ctagctagtt
     5401 taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg atgttgttaa
     5461 tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa gctgttcttc
     5521 acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg agagtgctga
     5581 cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac atcctagcga
     5641 ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg attctcgcat
     5701 ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt attttaagaa
     5761 gagatgttga ttttatcggg tataaccgga actttacaat catcgattct tctgaacagc
     5821 tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa tatgatcctc
     5881 gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct caggattttg
     5941 caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct gcttaccaaa
     6001 aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat acgattcgat
     6061 tatttgaaga acatcctgat tcactcacgt attatcaaaa caaattccat tatattcatg
     6121 tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta ttggcaggta
     6181 gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc tggcgcggtg
     6241 cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca gtaattttgc
     6301 tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa gtcattgaaa
     6361 acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga aacaagatca
     6421 cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagac cggatgcaag
     6481 aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac cggacgaacg
     6541 cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat aaaatggttg
     6601 gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat ttgaatgtgc
     6661 tggtaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc aaacgaggca
     6721 tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa tggccactgc
     6781 ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga cagcaattag
     6841 gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg acagtgacag
     6901 aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa atccaaaata
     6961 cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc acacaagaat
     7021 tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa gaaaaactaa
     7081 cggtcttctt aaatgatctg gcacttgttt ccgacattga caatttagaa gaagatgctt
     7141 cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaatttcca gttgtattcc
     7201 tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa gaaagcgaac
     7261 tggaggaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa gcgttgtatt
     7321 taacaaatgc tttctccaga acattatatg gacggacgca atataaccga ccttctcgtt
     7381 ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct acgcctaaaa
     7441 aaacgcctgt attcgctaaa aaaacagcat atagctataa acagccagag acagcggttg
     7501 ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga gataaagtca
     7561 aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg aaggatctag
     7621 aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc tttgcgccaa
     7681 ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat tgacgaagct
     7741 gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga atactacgta
     7801 gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga attagtagca
     7861 atcgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg agtaggcgga
     7921 aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag tttgaacgac
     7981 gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc agtgggacgt
     8041 gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt gcgttatgaa
     8101 gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga aaacatcact
     8161 gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat gaacatcgaa
     8221 gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca agacagagaa
     8281 gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc tttgcgtcag
     8341 ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac agtagctgat
     8401 ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga aaaaattgga
     8461 ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg gtcatacatt
     8521 gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat cgtcatcaaa
     8581 gtgaatgaat tctcgctaca agatcaactt ggttttacta tcaaagcacc gagatgggca
     8641 gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat cgaatggaca
     8701 gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg agtagcagga
     8761 acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa ggatatccgc
     8821 ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt ttcgcaagtc
     8881 gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca ttgtccagtt
     8941 tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat caatcctaaa
     9001 tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc gatgaatatc
     9061 gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt agcagatgtt
     9121 gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat aaaagaaaaa
     9181 tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt cgagcggttg
     9241 attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt agcagaacat
     9301 ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc gcttgatacg
     9361 atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga agtccatgaa
     9421 ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct tcgaaatgcc
     9481 caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac tggtaaacta
     9541 acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg gaaagtgact
     9601 ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg cagcaagctg
     9661 accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc agatgcattg
     9721 gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat ttcaataaac
     9781 aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac tcgtttagaa
     9841 agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa attagcaaaa
     9901 cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa aatcatcgat
     9961 atggttgaaa tgctggaaga agtagatacg gaaggtgttc catttacgtc gaatgtggtt
    10021 gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg cgatgaatta
    10081 ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat catcgataat
    10141 ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa ctgcatcata
    10201 tgcttgtatc gaaagaaatc actgtacaag atttgacaaa aacaacattt gaacgaatca
    10261 aagaaacaga gcctgaggtc gaggcattca ttacattgaa cgaagaaaaa gcattggaac
    10321 aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct ggtattccaa
    10381 ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca tctaagatcc
    10441 tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat caagcagata
    10501 tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt acagaaactt
    10561 cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt ggctcttctg
    10621 gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt agtgatactg
    10681 ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa cctacttatg
    10741 gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa atcggaccaa
    10801 tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt tatgacgaaa
    10861 aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg acaggcgaca
    10921 tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg gcccctggcg
    10981 taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct gttgtggaag
    11041 aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt gcctcatcag
    11101 aagcaagttc caatttgcaa cgattcgatg gtatccgcta cggttatcgc tctgaaaatg
    11161 tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt gaagaagtaa
    11221 aacgccgtat catgttaggt acattctcct taagcgcggg ttattatgac gcttacttca
    11281 aaaaagcagg acaagtccgt acattgatca aacgagattt tgaaaaagtc tttgcagatt
    11341 atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt gagaacatta
    11401 acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac ttggctggac
    11461 ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga ttgcagatca
    11521 tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt gaacaagcaa
    11581 cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga attttgaaac
    11641 agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt tctctccagc
    11701 gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact ggggttatcc
    11761 aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag cagctttggc
    11821 attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact tttacccaga
    11881 taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg acggatggat
    11941 cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc atttagaaga
    12001 agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt tgaaccgaca
    12061 aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag aagaagcata
    12121 cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg atgtgaaaat
    12181 ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg gacaagaaga
    12241 gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga aaaaaggttt
    12301 agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa tccaacaaga
    12361 aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca aagaagggtc
    12421 tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg atgacgaatg
    12481 gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg aacgctatat
    12541 ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa aagaaatgtc
    12601 tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat ctaactggtt
    12661 gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg agacaaaatt
    12721 gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga tcagctcaaa
    12781 aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc aagaagtagt
    12841 tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa tcattaatga
    12901 aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag atcgtgcggt
    12961 tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc ctggtgtcgt
    13021 gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga aaaaagcacg
    13081 cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg cggatatcct
    13141 acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac cagaaccaga
    13201 ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag tagtggcagc
    13261 tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg aacaccgtcc
    13321 taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat taaaaattcc
    13381 tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg taaaaatgga
    13441 catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct atttgacaga
    13501 attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg cttatcttgc
    13561 gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga agtatgatga
    13621 aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact ctgttggagg
    13681 attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt taatcattgt
    13741 gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga atggcggtcg
    13801 acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg ttgaaacaga
    13861 agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg gggaagcacc
    13921 gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg atcagattcc
    13981 tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca aagaattcgt
    14041 aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa tagcagacga
    14101 aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg tcagtgccgg
    14161 ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa aataggctta
    14221 aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata tacagtcgaa
    14281 atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta tccattattt
    14341 atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc aggcaataag
    14401 tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca gcctgtagaa
    14461 aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta tgaacaacag
    14521 cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa gatgccagaa
    14581 gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa taaagcacag
    14641 atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa gaatagccat
    14701 gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca agcaatcaaa
    14761 gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga aaaaaatgaa
    14821 ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat gatggtcgtt
    14881 ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc tattcatgaa
    14941 gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac aaatgtgatt
    15001 ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca gctattagga
    15061 aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca agcagagtat
    15121 ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt gatcgacgct
    15181 tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa ggtgtatggg
    15241 atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa taataaaatc
    15301 gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg gcaagaaaaa
    15361 gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga tcatcatttt
    15421 attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg taatccggca
    15481 acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa gaaagttcag
    15541 cctgtagatc ttttcccgca aacccatcat atcgaattag tcgcttcatt cgttaaagga
    15601 gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa caaggtggag
    15661 gccgatccaa acaaatggaa acacctaaga atgatcgtaa aaacatgaga aaaggcccta
    15721 aaatctttaa taaatagacg tgctaaagaa agactaagaa gtgttaactt ttgttattgt
    15781 ttcttagtct ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag
    15841 ttgtgcaaaa tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt
    15901 tcaatttgat taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa
    15961 cactaaatat acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga
    16021 tgacgacgta gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat
    16081 acgatagtga acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag
    16141 ttgtcttaga ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt
    16201 gaccggatct acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa
    16261 tgtggatttg cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt
    16321 cttttgtcaa aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc
    16381 ttgataggaa ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc
    16441 ttatgaaaaa gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg
    16501 cgtttgcaat ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc
    16561 atatgttttc aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa
    16621 acgtggcgaa aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca
    16681 tctactatcc aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag
    16741 gattcgtcgg cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg
    16801 ccaacatcgc agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc
    16861 aagttcttca agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat
    16921 tgctagacat ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc
    16981 aatatgcagc tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac
    17041 ttccaaaaga tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa
    17101 cagcacagca gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt
    17161 cgttgattgg aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg
    17221 accatgtttc taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc
    17281 aggagcaagg tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct
    17341 tgttttatac agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa
    17401 aagaatcgca aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg
    17461 ttgtgaaaaa gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc
    17521 tttcaatacc tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa
    17581 gtcaaaatga ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct
    17641 gcttgcccaa ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg
    17701 attgtctcga tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt
    17761 tgtttcaatg cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta
    17821 ggaagagggg attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc
    17881 cagccaccta gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga
    17941 taggcacgca tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg
    18001 actttatatc ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt
    18061 acggttttag tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca
    18121 gggttatcca cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc
    18181 gtaacagtcc caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga
    18241 ttatcagata gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc
    18301 atccctactc gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta
    18361 gagatcacac caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa
    18421 tccaacgcag cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc
    18481 tgatcgttat tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca
    18541 tctctaattc caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa
    18601 attgtttagt aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg
    18661 ggagtgaaat gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg
    18721 aaaagagccg agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag
    18781 aaagcaatgt attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata
    18841 aaaattatcc ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt
    18901 ctctgtcgaa agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa
    18961 cgcccgcatc gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca
    19021 gccattatat tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg
    19081 acaagtcaga ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt
    19141 tcgatttgtt ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa
    19201 gaaatgaaac cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg
    19261 cccatactag gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac
    19321 agatttcacg gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga
    19381 gcagtcacca tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg
    19441 gagaagcgat cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag
    19501 atcagatcaa tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat
    19561 tgaatgcagg ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat
    19621 tgaacaaaat gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc
    19681 atcaatttgc tgaacggccg attctggtca ataactggga agccacttac tttgacttca
    19741 atagtgaaaa aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg
    19801 tattagatga tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt
    19861 tcgagtatga aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca
    19921 aaggattgaa atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt
    19981 atcgaacaca tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat
    20041 cacaacatgt actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga
    20101 gaaagatttt agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt
    20161 cagatgtcta ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata
    20221 tgctcgggct ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg
    20281 aaggctgttc tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt
    20341 catggacaag tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc
    20401 ttgcttatcc gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg
    20461 acagatctac ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg
    20521 aactggattt gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat
    20581 cctatcaaac gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt
    20641 ttgaagaaaa tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt
    20701 ttttagggag gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt
    20761 tagatgatga ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat
    20821 tgatgacagt tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc
    20881 atttcaaaaa gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga
    20941 tgatccacaa gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc
    21001 aacaagggaa gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg
    21061 tttttgttat tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga
    21121 tgcctgttct tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa
    21181 gatccattac atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt
    21241 cacgacaagc cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt
    21301 tggttcgtat ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat
    21361 tcattttgct ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta
    21421 tgccgaagat tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa
    21481 tattctagct gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt
    21541 tttccgattt tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt
    21601 agaagatctt tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt
    21661 agatttgtct tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc
    21721 tgatgggaag gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat
    21781 cgatggattt gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga
    21841 attggagacg atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt
    21901 tcgtaaaatc gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa
    21961 cggggtaaat cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat
    22021 ggaaaaagat ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta
    22081 ccccaaccag atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga
    22141 aacgaacctc gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa
    22201 cgtaccaggt gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt
    22261 cgaaacatta aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc
    22321 ggaagaaaac attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt
    22381 ccattatgaa gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag
    22441 ctgcatgtat gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc
    22501 gttcatctta tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta
    22561 catgacgttg cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga
    22621 tcaggcgatc caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga
    22681 ctttgacgat aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg
    22741 acaagaaaaa cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa
    22801 gtcatatgga tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat
    22861 cggggaatac gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa
    22921 acggaaaaga atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata
    22981 atgacagagg tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc
    23041 caaaagtcaa agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc
    23101 cagtaaataa tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac
    23161 tggaaatcaa cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg
    23221 gtaaaatgct tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat
    23281 tgggtttaag gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag
    23341 gggaaacata tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac
    23401 tccccgtttc cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg
    23461 tagaactaac aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt
    23521 tgcgatttga acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag
    23581 aactggaaca ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga
    23641 tttttgcaaa agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac
    23701 cttaccgaat cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt
    23761 tagattatta tttgaaaaac ggagtttaca agacaaagct gctttattct atatgtcatg
    23821 aacaag
//
LOCUS       DADJYG010000026        23592 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.026, whole genome shotgun sequence.
ACCESSION   DADJYG010000026 DADJYG010000000
VERSION     DADJYG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.026"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            35..1375
                     /locus_tag="JZ382_000871"
     CDS             35..1375
                     /locus_tag="JZ382_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2427074.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1593..2036
                     /locus_tag="JZ382_000872"
     CDS             1593..2036
                     /locus_tag="JZ382_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAY2427075.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="JZ382_000873"
     CDS             complement(2540..3958)
                     /gene="nhaC"
                     /locus_tag="JZ382_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAY2427076.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4361..5584)
                     /locus_tag="JZ382_000874"
     CDS             complement(4361..5584)
                     /locus_tag="JZ382_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAY2427077.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5615..6454)
                     /locus_tag="JZ382_000875"
     CDS             complement(5615..6454)
                     /locus_tag="JZ382_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY2427078.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6471..7151)
                     /locus_tag="JZ382_000876"
     CDS             complement(6471..7151)
                     /locus_tag="JZ382_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427079.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7153..8226)
                     /locus_tag="JZ382_000877"
     CDS             complement(7153..8226)
                     /locus_tag="JZ382_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2427080.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(8873..9544)
                     /locus_tag="JZ382_000878"
     CDS             complement(8873..9544)
                     /locus_tag="JZ382_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAY2427081.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="JZ382_000879"
     CDS             complement(9631..12309)
                     /gene="mgtA"
                     /locus_tag="JZ382_000879"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY2427082.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTRFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12968..13405
                     /gene="ndk"
                     /locus_tag="JZ382_000880"
     CDS             12968..13405
                     /gene="ndk"
                     /locus_tag="JZ382_000880"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY2427083.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13427..14293)
                     /locus_tag="JZ382_000881"
     CDS             complement(13427..14293)
                     /locus_tag="JZ382_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2427084.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14453..16546)
                     /locus_tag="JZ382_000882"
     CDS             complement(14453..16546)
                     /locus_tag="JZ382_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY2427085.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17295..17918)
                     /locus_tag="JZ382_000883"
     CDS             complement(17295..17918)
                     /locus_tag="JZ382_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427086.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18077..19474)
                     /locus_tag="JZ382_000884"
     CDS             complement(18077..19474)
                     /locus_tag="JZ382_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2427087.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19506..20477)
                     /locus_tag="JZ382_000885"
     CDS             complement(19506..20477)
                     /locus_tag="JZ382_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2427088.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20851..21276
                     /locus_tag="JZ382_000886"
     CDS             20851..21276
                     /locus_tag="JZ382_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427089.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21764..22465)
                     /locus_tag="JZ382_000887"
     CDS             complement(21764..22465)
                     /locus_tag="JZ382_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAY2427090.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22481..22921)
                     /locus_tag="JZ382_000888"
     CDS             complement(22481..22921)
                     /locus_tag="JZ382_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAY2427091.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22921..23496)
                     /locus_tag="JZ382_000889"
     CDS             complement(22921..23496)
                     /locus_tag="JZ382_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAY2427092.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         6781 a         4896 c         4063 g         7852 t
ORIGIN      
        1 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
       61 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
      121 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
      181 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
      241 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
      301 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
      361 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
      421 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
      481 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
      541 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
      601 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
      661 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
      721 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
      781 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
      841 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
      901 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
      961 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
     1021 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
     1081 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
     1141 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
     1201 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
     1261 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
     1321 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
     1381 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
     1441 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
     1501 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
     1561 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
     1621 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
     1681 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
     1741 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
     1801 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
     1861 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
     1921 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
     1981 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
     2041 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
     2101 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
     2161 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
     2221 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
     2281 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
     2341 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
     2401 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
     2461 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
     2521 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
     2581 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
     2641 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
     2701 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
     2761 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
     2821 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
     2881 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
     2941 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
     3001 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
     3061 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
     3121 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
     3181 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
     3241 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
     3301 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
     3361 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
     3421 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
     3481 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
     3541 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
     3601 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
     3661 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
     3721 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
     3781 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
     3841 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
     3901 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
     3961 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
     4021 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
     4081 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
     4141 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
     4201 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
     4261 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
     4321 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
     4381 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
     4441 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
     4501 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
     4561 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
     4621 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
     4681 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
     4741 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
     4801 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
     4861 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
     4921 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
     4981 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
     5041 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
     5101 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
     5161 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
     5221 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
     5281 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
     5341 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
     5401 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
     5461 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
     5521 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
     5581 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
     5641 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
     5701 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
     5761 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
     5821 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
     5881 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
     5941 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
     6001 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
     6061 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
     6121 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
     6181 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
     6241 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
     6301 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
     6361 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
     6421 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
     6481 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
     6541 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
     6601 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
     6661 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
     6721 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
     6781 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
     6841 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
     6901 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
     6961 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
     7021 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
     7081 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
     7141 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
     7201 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
     7261 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
     7321 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
     7381 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
     7441 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
     7501 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
     7561 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
     7621 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
     7681 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
     7741 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
     7801 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
     7861 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
     7921 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
     7981 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
     8041 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
     8101 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
     8161 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
     8221 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
     8281 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
     8341 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
     8401 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
     8461 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
     8521 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
     8581 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
     8641 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
     8701 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
     8761 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
     8821 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
     8881 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
     8941 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
     9001 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
     9061 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
     9121 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
     9181 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
     9241 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
     9301 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
     9361 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
     9421 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
     9481 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
     9541 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
     9601 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
     9661 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
     9721 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaccttg taaaaggaag
     9781 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
     9841 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
     9901 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
     9961 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    10021 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    10081 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    10141 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    10201 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    10261 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    10321 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    10381 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    10441 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    10501 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    10561 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    10621 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    10681 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    10741 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    10801 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    10861 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    10921 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    10981 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    11041 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    11101 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    11161 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    11221 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    11281 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    11341 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    11401 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    11461 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    11521 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    11581 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    11641 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    11701 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    11761 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    11821 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    11881 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    11941 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    12001 atctccagga gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa
    12061 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    12121 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    12181 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    12241 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    12301 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    12361 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    12421 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    12481 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    12541 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    12601 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    12661 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    12721 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    12781 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    12841 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    12901 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    12961 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    13021 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    13081 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    13141 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    13201 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    13261 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    13321 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    13381 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    13441 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    13501 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    13561 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    13621 gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    13681 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    13741 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    13801 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    13861 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    13921 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    13981 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    14041 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    14101 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    14161 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    14221 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    14281 caccatcata cataatagtt ctccttttta atttaaattt ttccacattc aataaataga
    14341 tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac
    14401 caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat
    14461 tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat
    14521 taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat
    14581 tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg
    14641 gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct
    14701 tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga
    14761 tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa
    14821 agttcccatt tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa
    14881 aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga
    14941 tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca
    15001 atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa
    15061 gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca
    15121 caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg
    15181 attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca
    15241 aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa
    15301 gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata
    15361 aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat
    15421 caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta
    15481 cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg
    15541 cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg
    15601 tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga
    15661 aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt
    15721 cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga
    15781 tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct
    15841 ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag
    15901 tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa
    15961 tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta
    16021 gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga
    16081 cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca
    16141 cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg
    16201 acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga
    16261 attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca
    16321 aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata
    16381 aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta
    16441 atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca
    16501 aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc
    16561 tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt
    16621 atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac
    16681 cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac
    16741 tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa
    16801 ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa
    16861 acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc
    16921 tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag acctcttgat
    16981 acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct aattttacaa
    17041 cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    17101 aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac ggcaggctca
    17161 agacctcttg atacacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca
    17221 aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
    17281 acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta
    17341 ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa
    17401 ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca
    17461 tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa
    17521 taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa
    17581 agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt
    17641 acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat
    17701 tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt tgagaattct
    17761 tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca
    17821 agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg
    17881 aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa
    17941 ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat
    18001 cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc
    18061 tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat
    18121 gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat
    18181 atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc
    18241 ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa
    18301 attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt
    18361 ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat
    18421 tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt
    18481 tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat
    18541 cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat
    18601 ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa
    18661 gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga
    18721 atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt
    18781 cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca
    18841 tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga
    18901 tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga
    18961 ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa
    19021 aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt
    19081 tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt
    19141 tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc
    19201 cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag
    19261 tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag
    19321 gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct
    19381 ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc
    19441 accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt
    19501 tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc
    19561 tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt
    19621 ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag
    19681 tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca
    19741 ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct
    19801 tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc
    19861 gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct
    19921 gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc
    19981 acttttgcta cactttctcg atcattcact aatattgtat caatacctgc tggtccttct
    20041 ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac
    20101 tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt
    20161 ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt
    20221 tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg
    20281 tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt
    20341 tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt
    20401 tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca
    20461 tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt
    20521 tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa
    20581 ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac aagtatttga
    20641 accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt
    20701 tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt
    20761 gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat
    20821 ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg
    20881 tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg
    20941 gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta
    21001 tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat
    21061 aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct
    21121 gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga atatattatt
    21181 tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt
    21241 ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg
    21301 tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg
    21361 gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt
    21421 taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt
    21481 taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa
    21541 tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt cttcctttga
    21601 tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag
    21661 atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac
    21721 atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac
    21781 cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta
    21841 atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga
    21901 atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac cattgattat
    21961 agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat
    22021 agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga
    22081 ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta
    22141 ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat
    22201 ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag
    22261 catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact
    22321 gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg
    22381 gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg
    22441 ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt
    22501 aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat
    22561 acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga
    22621 tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg
    22681 tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat
    22741 ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt
    22801 gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac
    22861 gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca
    22921 ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta attcattgac
    22981 cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc
    23041 ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa
    23101 tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa
    23161 ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa
    23221 taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg
    23281 caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag
    23341 tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat
    23401 ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt
    23461 accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa
    23521 gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta
    23581 gtggtatata gg
//
LOCUS       DADJYG010000027        23272 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.027, whole genome shotgun sequence.
ACCESSION   DADJYG010000027 DADJYG010000000
VERSION     DADJYG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.027"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            30..1031
                     /gene="ccpA"
                     /locus_tag="JZ382_000890"
     CDS             30..1031
                     /gene="ccpA"
                     /locus_tag="JZ382_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAY2427093.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(1368..3713)
                     /locus_tag="JZ382_000891"
     CDS             complement(1368..3713)
                     /locus_tag="JZ382_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAY2427094.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            3992..4894
                     /locus_tag="JZ382_000892"
     CDS             3992..4894
                     /locus_tag="JZ382_000892"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY2427095.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            5107..6882
                     /locus_tag="JZ382_000893"
     CDS             5107..6882
                     /locus_tag="JZ382_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAY2427096.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6948..8534)
                     /locus_tag="JZ382_000894"
     CDS             complement(6948..8534)
                     /locus_tag="JZ382_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAY2427097.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8785..9009
                     /locus_tag="JZ382_000895"
     CDS             8785..9009
                     /locus_tag="JZ382_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAY2427098.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            9151..10902
                     /locus_tag="JZ382_000896"
     CDS             9151..10902
                     /locus_tag="JZ382_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2427099.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            10902..12686
                     /locus_tag="JZ382_000897"
     CDS             10902..12686
                     /locus_tag="JZ382_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427100.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            13012..13863
                     /locus_tag="JZ382_000898"
     CDS             13012..13863
                     /locus_tag="JZ382_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2427101.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            13917..15668
                     /locus_tag="JZ382_000899"
     CDS             13917..15668
                     /locus_tag="JZ382_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2427102.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            15672..15842
                     /locus_tag="JZ382_000900"
     CDS             15672..15842
                     /locus_tag="JZ382_000900"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427103.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(15882..16784)
                     /locus_tag="JZ382_000901"
     CDS             complement(15882..16784)
                     /locus_tag="JZ382_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2427104.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            17015..18418
                     /locus_tag="JZ382_000902"
     CDS             17015..18418
                     /locus_tag="JZ382_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY2427105.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            18418..19851
                     /gene="ascB"
                     /locus_tag="JZ382_000903"
     CDS             18418..19851
                     /gene="ascB"
                     /locus_tag="JZ382_000903"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY2427106.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19996..20922
                     /gene="trxB"
                     /locus_tag="JZ382_000904"
     CDS             19996..20922
                     /gene="trxB"
                     /locus_tag="JZ382_000904"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY2427107.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            21136..22863
                     /locus_tag="JZ382_000905"
     CDS             21136..22863
                     /locus_tag="JZ382_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAY2427108.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            22935..23228
                     /locus_tag="JZ382_000906"
     CDS             22935..23228
                     /locus_tag="JZ382_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY2427109.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
BASE COUNT         7405 a         4147 c         4961 g         6759 t
ORIGIN      
        1 acatataaaa tatgaatagg agaacacaaa tggaaaagca aacaatcact atctatgatg
       61 tagcgagaga ggcaaatgtt tcgatggcta ctgtttcacg tgtcgtaaat ggcaatccaa
      121 acgttaaacc agcaacaaga aaaaaagtct tggaagttat cgatcgcttg gattatcgtc
      181 caaacgcagt tgcgcgtggc ttggcaagta agaaaacaac aacagttggt gtaatcatcc
      241 ctgatgtcag caatatgttc tttgcttctt tagcacgtgg tatcgatgat gtggcaacaa
      301 tgtacaaata caatattatc ttagcaaatt cagatggcaa cgaccaaaaa gaagtcaatg
      361 tattgaataa cttactggct aagcaagtgg acggcgtgat cttcatgggt caccatatca
      421 cagacgaaat ccgtggagaa ttttcacgtt caaaaactcc tgttgtatta gctggctcta
      481 ttgatccaga tgaacaagta ggtagcgtaa acattgatta tgtatccgca acaaaagatg
      541 cagtaaacaa attggcaaaa agcggcaata aaaaaatcgc ttttgtcagt ggtgcgttga
      601 tcgatcccat caatggacaa aatcgtctga aaggctataa agaagcatta agcgaaaata
      661 acctaccata tagcgaagga ttgatctttg aagcacaata caacttcaaa gatggtctat
      721 ctctagtcga tcggatccat aatagcggtg caacagctgt ctatgtttca gatgatgaat
      781 tagccatcgg tattttggat ggtttattag accgtggcgt gaaagtaccg gaagaattcg
      841 aaatcatcac aagtaacaat tcactactga cagaagtagc tcgtcctcgt ttaacaagta
      901 tcacacaacc attatacgat ttaggggcgg tatctatgcg cctattgacg aaaatgatga
      961 ataaagaaga agtcgaagaa aaaacgatta tcttgcctta tggaatcgaa gaaaaaggat
     1021 ctacaaaata ataaaaggac aaccattcag aggttgtgtg cctgccgttt aactctgcgt
     1081 tacaaggagc aatttcaaaa aacagcatct catgtttgat gaatattgtg actcgtgatt
     1141 cggagttggc aggagaacac cgtttatttg ggaaaagggg ttatgacaaa attttgttat
     1201 aatccttttt ttgtgtctta aaaaagagag aaagtggttg aaattacatt tatcgtatat
     1261 aaaacaatcc gtgtatagat gctgcatttt gttcaccacc atcaaaaaaa taagggactg
     1321 tgacaatact attgtcacaa tcccttatta ctgtgtattc tattaaatca atcatctttc
     1381 tcatctgatt ctttagttgc ttgattcgct cttgcataag ctgaagcggt gttccagtaa
     1441 tctgtatagt tttcatcggt accgccaacg ccaaatttaa aggcgctttt cttcggtcca
     1501 tttttcgccc atagcgattc aactgttttt cctggtgttt ggatggattt tttattgaca
     1561 gtaacttttc caggtgtttg accagtaaat ccagatactt ttttcttcat cacatcagat
     1621 gataattcga atttctcatc agtgccaaaa atgtcaggtt ttgcttggta agcttgattg
     1681 atcagattgg caagataatt agccgaacgt atccttgctt tgctgtccat tccagtattg
     1741 tcatcgtgcc ctgcccagct actgatggta atagacggag tagagacaat tagccatgaa
     1801 tctcggtatt cattggtaga accagttttt ccgacccaat ctgcttttcc tagatttccg
     1861 tttaagcttg agatggcatc tttgaatggt gtagtgattt ttgcattgat cacactacgc
     1921 atcatgtcat tcatgatcga tgccgttgct tccgaataga tccggacaga gttgctttcg
     1981 tgtttataaa ttacatttcc tgcgttatct gtgatggaat cgatgatata gccttcttca
     2041 tagacgccgc cgtttgctaa agcctgaaat ccgttggtct gttgaagcgt cgtgacatct
     2101 actgtaccta aaggagcaga ttcaacgccc caattgtcat ttgccgggta gttcatcttt
     2161 gccaagtagt gctcgtaagc atattgtttc gatccaccgg aattcaacac atcttgatat
     2221 aagtgataag ccgggatatt gttggaccat tctagtgact cacggacggt ttggaaggtg
     2281 ttcgatcctt cgttagtagc attgacgatt ttctcgccag cattttctcc ttcttgccaa
     2341 gttgttgcat aatctgatac acgggattct gatccgataa gccccatgtc gattgcaggt
     2401 ccataaacaa gaactggttt gattgatgag ccagcttgac gttctgtatc gaaggcatgg
     2461 ttattttgat tctgactgta attccgtccg ccaacgaatc cgtagatgcg gccagttctg
     2521 ttatccatca atacatttcc ggtttctact tgactgccag caccatcatc taacatgtat
     2581 ccataatttt caacgccatt ttgcaatgca gcatagacat ctttatcgat tgtcgaatgg
     2641 attgtatatc ctttattttg gatcgtttgc tgtgcctttt gataataagc gtcactgacc
     2701 gatgaatcag acatatcagc gttgtctttt tcagccaatt gttgtgcaat gattcttgtt
     2761 gcttcattca tcacagtata atacaagaat tctctgtcag tctgttctgc gatttgttgt
     2821 ggtagaaagt cttttgtaag gtcataggct tttgcttctt cgtattgttc tttcgtaatt
     2881 tggccttcac gatacatact gaataaaaca aaatcttttc tggcaaggcc ggcagaaaga
     2941 tcttctttta gcgcgcctgt gttcgtgtat ggactatacg tgatcgggct ttggggtaat
     3001 ccagcgatat aagcagcttg cggcagcgtc acatcttttg catctacacc aaagattcct
     3061 tttgcagctt cctggactcc agcaatgttt tgtcccttat tattacgacc aaatggtgag
     3121 acattcaaat acgtagcaat gatctcatct tttgaaaagt atttttctac ttgtgcagaa
     3181 agaatgatct cgtttgcttt acgtttgaat gttgtttcat ctgtaaggat ctgctgtttg
     3241 actaattgct gtgtcaaagt tgatccacca gaagagccaa taccagtagc ttcagatact
     3301 aatgctcgta aaacagcttt aggaacgtaa ccttggtgtt tatcgaagta ttcatcctct
     3361 gtgctgataa ttgcagtttt caataaaggt gaaatcttat cagatgaaat agtcgtgcgc
     3421 atcagatctg tctggatttt tgatatctct gtattatctg cataaacaat ctttgacgtt
     3481 tctgtaatgt ttcctagttc tgtctgcagt gcttttttag tcggcaattg tgtgtcttcg
     3541 acgagatagg caaaatagcc agcgcctatt ccaataccta gagctgcgaa catcaaaaga
     3601 agaacagtaa taaaaacagt caatgattga aaaactctaa aaataatctt aggaacaaac
     3661 catttttctt tttttcttct attctgtttt tttcgagtat ttttgcgtgg caaaagggtc
     3721 acctcaacta atggttttat ttcgctaatt atagcaaata atagaacaaa aaaacagaca
     3781 aggctgtttt tggcaggaat ttctttttta aaagagttaa aacgtgagaa aatgatcttt
     3841 attataaaag agttcttaag cttttcttta gagaaaattt cagttactat ggagaacagg
     3901 gcaaagaaaa atcaatcatt ttctttgtat atcatcttac caatatatag gaattgttag
     3961 taacaattgc ctttcaagga ggaatccatc catgttatta ggctcacacg tcagcatgag
     4021 cggaaagaaa atgctgttag gagcagcaga agaagcagcc agttatggat cttcgacttt
     4081 tatgatctat acaggcgccc ctcaaaacac tagaagaaaa gccatcgaag agatgaatat
     4141 tgatgccgga aaagcgtaca tggcagaaaa aggtctaaca aatatcgtcg ttcatgcccc
     4201 ttatatcatt aatttgggga atacgatcaa accagaaatg tttccttttg ccgtccaatt
     4261 tctgagagaa gagattgttc gtgcggaagc tttaggcgct actcaaatta cacttcaccc
     4321 aggtgctcat gtaggggcag gaacagaagc cggaattgct agaattattg aaggcttgaa
     4381 tgaagttttg acaaaagaac agacggcaca aatcgctttg gagacaatgg caggtaaagg
     4441 tacggaaatc ggtcgaacat ttgaggaatt agcgcaaatc atcgatggag taaaattgaa
     4501 tgaaaaatta tccgttactt ttgacacctg tcatacgaat gatgccggtt ataatattaa
     4561 agaagacttt tcaggtgtaa tggaagagtt tgataagatc atcgggttag atcggcttaa
     4621 agtactccat atcaatgatt ctaaaaatcc gcaaggttcg cataaagacc ggcacgccaa
     4681 tattggtttt ggaacaatcg gattcgacac gctgaataag atcgtccatg atccaatgtt
     4741 tgacaatatt tcgaaaatct tggaaactcc ctatgtaggc aaagataaaa agaaagctta
     4801 cgcaccatat ggaaaagaaa tcgccatgtt caaagcgcaa acattcgatc cagaagtgtt
     4861 tcctgagtta ttagcagaag aagtgatact ctaagatttt ctatctgaca gcagcaaaaa
     4921 aggaccaggc acattgcctt gtcctttttt gtttaagaaa caaaggagta ctttttgcag
     4981 tctttttcat catgcatctt ctcccccctc tttttaaatt tagacaaaca aggtatactg
     5041 gtagaaaagt aaaaaaacga aaagtttcac aagcaagtgg caaaacgtga ttggaaggac
     5101 gaaaagatgc tgaacacttt aaaacccttg atcaaccaac taaaaaagaa acatacatac
     5161 tttattcttg gcatagccct attatttaca attattcgtt tgtatttata tagtaaagca
     5221 atctatggaa cagacatgtc ggcaacctat gatgaccagc tgctgattcg ctatgctcaa
     5281 aatattttgg acggtaaatg gctaggggag tatacgacta caacactttc caaaggaatc
     5341 tcctacagct tatttttagt acttgcacat aaactgcatc tttcttatgc agttttgttc
     5401 ggcatgttca gtatttttgc cagtatattg gttgcactcg cgtttaaacc gattgtgaag
     5461 aacaagtatc ttcttttaag tatctatttt tattttttgt tttctccgat tagttttaca
     5521 catgaatatt ccactcgaac atatcgtaat gccctcgtga ttccggctgt tgagatcatt
     5581 atcgcttgtt tattagctat ttattaccgc aaatacggtt ctagcagaaa attgctgcca
     5641 tggtttttag ggctttctgt cgcttttcct ttcttttggt tcattcgcga agattctatc
     5701 tggcttcttc cttttactgt agtagctttg ggtatcagtt tcgtccagat cttgctaggg
     5761 caagactttt ctttcaaaga agtaaagaag ttaaaaaaac gactctttct tctctcgaaa
     5821 cgggaatggc tgaaactttc attgttttta cttcctatag ttggcttttc catcgtctat
     5881 caatggatcg aatataaaaa tgagacaaca tacggtattg caaccatcaa tgaccgttcc
     5941 agtggagaat ttggacgttt gatgaaacaa ctgatccgaa tagatgatga aacgaacttg
     6001 aataaagaaa acagtgagat atgggtctca catgctgcac taaataaagc attagccgca
     6061 tctcctacat tccatactgt ttctacccgt attgacgaat tatatacgac tcacgcgtgg
     6121 actcaaggtg gaaaagtaaa agaattaccc ggagatatca tcttttgggc attgcgagat
     6181 gtggtaagcg aatctggtta ttaccaaaac aatgcaaaag aaaccaatga tttttggaaa
     6241 aaagtcaacc aagaattaga agaagcctat cacaagggaa caatcaaaaa gaaaaaagag
     6301 ttctatctga tgggatcagg agatggaaag acgatgagtg atctgccaat cgtattcgat
     6361 ttccttcaat caggatacgc ttatgcatgg cgatataaag atttccatca aggaggaaat
     6421 tatagtttcg gaacgcaaga acaagtcaaa caggctcaag aattattaaa ccttccgcta
     6481 atggagaatt ggatcgatag taatgaaccg agaacaccta atttccaatt aacaaaatca
     6541 gcaaaagtcg cgaatgtcat cataaaaatt taccaagttc tggctattcc cgtgctgatt
     6601 gcttcaatag cagggatatt acttatgata ctaggaaccg tatttgcaaa agctgatcgt
     6661 gcctattata gttcctcttt gattgtggtc accggattaa tactgacaca attggttttt
     6721 cttttcggtg tctcatggtt ttgttcttat gcgccagaaa aaaaggacta ttttttaagt
     6781 gtctacactg gtgcaggcgt gccattaacg caattcatcg tagtaacaag catgctagtt
     6841 ttcagtaaat taaatctgaa aaaagcttta tcttctaaat aaaaagaccc ttggtagcta
     6901 gatttttaac ctaactatca agggtctttg tgtggaaaaa tcagttttta ttcgatttct
     6961 tctaccgttt cctgcaaaag ctgttcatat tttttgtttc ctttatataa atcaccaatc
     7021 gtccatgcca acaggagaag gacaagaaca atgattacat aagcgatgtg atcagccaat
     7081 gctagttgag cggaagtggc ttgctcgccg aagaaacttt cgatctgtcc tggtaatcct
     7141 cgtaagttca agtaagttag actaatcaca gagaaccagc caagaagttt tacgatgaat
     7201 gaatttttaa atcggctacc catctccgct tggctgttgg tcatcatcag taaaggaatc
     7261 atcgaaaacg gcaacgcaaa agccaagaac acctgggatt cattcattaa gttattcaaa
     7321 gcggcatgtt cttcgatcgt tccttttgta cttgtaaaca aaacacaaat caatacagga
     7381 atcacagaaa gcaatcgtgt gaccagtctt cttagccaaa tcgggatacg catatggatg
     7441 aatccttcca tgatgacttg accggtcaac gtaccagtaa tagtagagtt ctggccagaa
     7501 gcaagaagtg ctacagcaaa taaagtggac aagataccag attttgctac agcaatcaaa
     7561 ataccattgc tcatagctga tggatcagat aaagcttcat acagaccaaa aaaggaagga
     7621 tcttggacag aaccggtttt gaagacagcg acacccatga tcaatagcag tgcattgact
     7681 agaaaagcca ttgacaactg gatattagaa tcccaagtag aaaaacgtac agcttgtgct
     7741 acatcttctt cgttttggtg atcgatcttc cgagtttgtg aaactgctga atgaagatat
     7801 aagttatgcg gcatgactgt tgcaccaata atccctaaag ctccagtcag cggagtttcg
     7861 ccgccgatga ccggtgagtc tgagaatgta tgactgttag ggataaagcc agcaaataaa
     7921 ctgtgccaat ctgggttaga caagccaact tgatagacaa aaacgaataa aatgacgaaa
     7981 attaagcaga caaccagtgc ttcgatctta cgaaacccga cttttgtcag tagtaagaga
     8041 acgaaaacat ctaatacagt aataaagacg gcaatcacca atggaatatc aaaaagtaag
     8101 tagagggcga ttgctgcacc aatgacttct gcaatatctg ttgccataat ggctaattca
     8161 gtcaagatcc ataaaatgac acctaatgtc ttacttgtgc gtgctcggat tgcctgtgcc
     8221 aaatccattt ggctcacgat tcctaatttt gctgccatat actgtaaaag catagcaatc
     8281 agactagaca tcaaaataac tgacatcaat aaatattgga aattttgtcc tccggtgatc
     8341 gaagtagacc agttacccgg gtccatatac ccaactgcta ctaacgcacc aggtccagag
     8401 taagcaagaa gtgttttaaa aaaacttttc ccctttggta catcaactgt tccatttact
     8461 tcagctaaag ataaaccatt tgtatgttca ataagtcgat gcttatgaaa ttgtttattg
     8521 gattgattat tcaagaaaaa accacctttc ctatttatct acgattacat tctacacctc
     8581 aaaaagaaaa aattaaagaa aaagattcgg tacgcctaat tttttttcat ggcgcgtctt
     8641 gctagatgac gaaagaaatt ttgttctcct ctaaaaaaac cactattttt tgcatgaaac
     8701 taaagaatac gcctttcttt tttcaataaa ctttagtaaa ataagtcatt gagagcaatt
     8761 cggaaataga aaggagagat ttatatgtta tcaacaggta ttgtcgtact tattgctatt
     8821 attgctttgc taatcggcgc agtaggtggc tttttcctag cgcgtaaata tatgaaagac
     8881 tatttagaaa aaaatcctcc cgttaatgag gacatgttgc gatcaatgat gatgtcaatg
     8941 gggcaaaaac catctgagaa gaaaattcgc cagatgatgc agcaaatgaa gaaccaaggg
     9001 aaaaaatagg ttctgaccca acgacttttt acgggctgat tttttcagtc cgttttttat
     9061 ttcgtaattt gttagactat tcagattatt cagcaaaaaa gacaagcaca gacaatccta
     9121 ggctcttgat aaagacaaag gaggagtaaa atgtcgattt ttagaaaatt aggctggttt
     9181 tttcgtcagg aaaagaaaca ttatttaatt ggggttttct cgctattcgc agtggccttg
     9241 gtacagttag ttcctccaaa agtgattggg atcatcattg atgagatcgc cgataaaaat
     9301 ttatcaataa agatcatttt cgggtggatc ttcctattga ttatcgctgc ttttttacaa
     9361 tatctttttc gttatatctg gcggacgaat atttggggaa gtgcagctcg attggagaaa
     9421 gaacttcggc agcagttatt caaacatttt acagaaatgg acaatctctt ttaccaaaaa
     9481 tatcggactg gggatttgat ggctcacgca acgaatgatt tgaatgcgat ccagaatgta
     9541 gcaggagctg gtatcttgac ctttgcagat tcagtgatta cgggcggaac aacgatcatt
     9601 gcgatgattt tgtttattga ttggcgctta acactgattg cgctgattcc tctgcctttg
     9661 ctggccgtca cttcacgagt gctggggtca aaactccatg atgcttttcg tgattcgcaa
     9721 gaagcttttt ctagtatcaa tgataagaca caagaaagta tcactgggat caaagtcatt
     9781 aaaacatttg gacaagaaaa agaagatcta gcggattttg aagaaaagat tgaagatgca
     9841 attattaaaa acaaacgagt caatttttta gactccttat ttgatccatt cattaccttg
     9901 attatcggtc tgtcttatgt tgcaacaatt attttaggtg gaaagtatgt cttggaaggc
     9961 acgatctcga tcgggcagat ggtctcattt attagttata tcggtatgct tgtttggccg
    10021 atgtttgcga ttggtcgatt gttcaacgta ttggaaagag gaaatgcttc ttatgatcgc
    10081 gtcaatgaac ttttgcatga aaagacgcat atcgtggaac aactgggtgc agtgcagacc
    10141 ccggcaaaag gcgagctgaa tgtagctatt tcctctttta attatccgaa agaagaatca
    10201 ccagcattag aacaaatcaa gtttgcgatc caagaaggag aaacattagg tattgttggg
    10261 aaaactggtg cgggaaaaac gacgatcatc aaattactga tgcgtgaata cgatgattat
    10321 caagggaaaa tccaatttgg cggacgttcg atcaaagaat attcattgaa tgctttatta
    10381 ggatcaatcg gctatgtgcc tcaggatcac ttcttgtttt cgatgaccat tcgagataat
    10441 atccgttttt ctaatccaaa actttcagaa gaagaagtag agcatgcggc ttctttagca
    10501 tttatcaaca atgaaatcaa ggaattacca aaaggttatg ataccttagt tggagaacgt
    10561 ggagtctctt tatctggcgg acagaaacaa aggatttcga ttgctcgagc attgatcaaa
    10621 aatcctgaat tattaatatt agatgatgca ctctctgcag tagatgccaa aacagaagaa
    10681 gctatcttgc ataatttaaa agaaataaga aaagagaaaa caactatcat tgcagctcac
    10741 cgtttaagta gcgtgatgca tgcaaaagaa atcattgttg tcgatcaagg gaaaatcatt
    10801 gaacgaggaa cccatcatga gctgcttgca ctaggcggct ggtatgaaca aatgtgggag
    10861 aaacagcaat tagaagcgaa gatagaagga ggcgaggcct gatggatgaa aattatcaat
    10921 cggaatggac aaaatcgatt ccacttaaag aacagaaaaa gatcatcaaa cgactgatga
    10981 agttcgccaa accttttaga agaacgttca tcgttgctat cttatttgcg tttgctttgt
    11041 ctgtgatcaa tgtcctattg ccaaggatca tccaaacttt tatggatgat catctggcta
    11101 aacagtcggc tacgacacaa gttattttgt tttttgctgg tatgtactta tttggagtca
    11161 tagtaaaaag tatcatttgg tttttccaat ggtatttgta ttccatggcc tcattgaaga
    11221 cctatcagta tattagggta aaattatttg aaaagctgca tacactaggt atgcgttatt
    11281 ttgatcagac accggcagga tcaaccgttt ctcgagtgac gaatgacaca gaaacgctat
    11341 ttgaattttg gtacgtcttt ttgatggtat tgactgggat ctttgcagtc gtttcttcat
    11401 tccttgccat gtttcaaatc aatggagaaa ttgccttgtg gactttggtg tttttgccga
    11461 ttttaggaat cgtcatctgg tattaccaga aattcagttc tcgtatttac cgaaatatgc
    11521 gggaaaaatt aagtcagctg aacacgaaat tgaatgaatc gatttccggc atgcgtatta
    11581 tccagcagtt tcgccaagaa gctcgtttag ctaaagaatt tgaacaagtg aacaatgagt
    11641 atttagacac gcgttatgcg atgatcaaaa cgaattcgat tctgttaagt ccgatcatca
    11701 attttctata tgcattggcg attgcgttgg tgttgacttt gtttgggatc gatgcgttgc
    11761 attctcccgt agaagttgga ctgatctatg catttacgac ctatgtccag ggatttttta
    11821 atccaatgtc tcaaatgatg gactttttga gcatcttcac agatgggatg gttgctggta
    11881 gtcgtatctt gaagattatg gatacggaag aatatgcacc acaacaaaac cctggagcag
    11941 atcaaatgat tacttcaggt aagattgagt ttcgcaatgt aagcttttct tatgatggag
    12001 agcacaatgt attaaacaat atctcttttg ttgctaatcc gggagagacc gttgcattag
    12061 tgggacacac cggaagcgga aaaagctcga tcatcaacgt attgatgcgg ttttacgaat
    12121 tttatgaagg tcagatattg attgatgatc atgacattcg agaatatcca atggaagagt
    12181 tacgtaagaa aatggggctg gtcttgcaag atgctttctt gttttatggg gatatcgcag
    12241 gaaatattcg gatgatgaat ccagctatca cagacgaaca gatacgccat gcggctgagt
    12301 ttgttcaagc aaacaaattc atcgatgaat taccaaaagg atatcactca aaagtgatcg
    12361 aacatggtgc cagctattct agtggacaac gacagctgat cacttttgcg cgaacaatcg
    12421 tgactgatcc taaaattcta gtattggatg aagcaacagc aaatatcgat actgaaacag
    12481 aaggattgat ccaagaagga ttggcaaaaa tgcgtcaagg acggacaacg attgcgattg
    12541 cccatcggct gtcaacgatt cgagatgcag aattgatttt agttttagac aaaggtcata
    12601 tagtagaacg cgggaatcat gaaacacttc tagcgcaaaa tggtttgtat gcagatatgt
    12661 acaaactgca aagcagtggt atataaaaga tgttgtgaca gtagaatttt gacctaagca
    12721 ctagagtgaa aataaaacat agcttctcat atttttcatc tgtgagagtt agtgtcgtag
    12781 tcaccagatt taaaaaaaga ttcgacagct agtcagaagt gcctaatagc cactaagaaa
    12841 gcgttgacaa aagcatttgc tttcgataat ataaagaaaa cgatacaact tggatagtga
    12901 ttgcctgatg caagctaaac ctgatgagtc tcccatggtt tcatggacgg ctttttgggt
    12961 ttttcttttt gttttggata cagccctata catcatggaa aggagcaaaa catgctgatt
    13021 gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca ggaaatgatc
    13081 gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt tgactcatgc
    13141 atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca agaattggca
    13201 caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata tacaaaagag
    13261 gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca tttgtatacg
    13321 gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta tgatatcaaa
    13381 caactatttc ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat ctcggataaa
    13441 taccacatca agttatcaga agatgaagcc ggatttatcg cattgcattt agtcaatgca
    13501 caaggcattc aaagccagca agacaatacc tacctgttta ctataacaat acaggatatt
    13561 ctaaatatcg tccgctatta ctataagatt acatttgatg aagattcagt ccattttttt
    13621 cgattcgtca cacacttacg tttttttctg acacgcgtcc tagatggaga aaaatcagaa
    13681 gtgggagaag tttctgagga attgtttcag gttattcaaa aaaaatataa ggatacgttt
    13741 ggttgcgttg agaaaatagc agacttttta gccgagacgt tcaactacca gatgtccacg
    13801 gacgaaaaaa tgtatttaac gatccatact gcgcgtttag tgcatgagaa tcgttgtaaa
    13861 taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac attcagttgg
    13921 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa gaattagcga
    13981 gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
    14041 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
    14101 tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc attgggaatc
    14161 atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt gatgaggctg
    14221 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
    14281 agccattcct tgctatccta gctgctggcg ggatgattcg tggactgaca gcattccttg
    14341 tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
    14401 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
    14461 taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc ttgtcgctgg
    14521 aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc actatttttg
    14581 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
    14641 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
    14701 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
    14761 tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat attttaatgg
    14821 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
    14881 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
    14941 aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg tcttttggac
    15001 aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
    15061 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
    15121 cattaccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
    15181 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
    15241 ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg attgggatcg
    15301 ccattgcgat ggtggtatcc tttacgctga cttttttctt ttggaaggat gaagcaggtg
    15361 aaacagatga tatacagaaa aaatcaggta aagaaattgt aaaaacaccg atacaaggac
    15421 aaatcgctcc actaaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
    15481 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga
    15541 ccctgttccc gacgaaacag gcaattggaa tggtttctga aacagggctg gaattgctga
    15601 ttcatgtcgg attggatact gtgcagttgg aagggaaata ttttgaaagc ttagtgcagc
    15661 aaggataaag agtgaaacaa ggcgatctgt tgctacgctt cgacaaaaag aaaatcgaaa
    15721 aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata
    15781 ttttggaaaa ccaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct
    15841 gaacggttag gtgaagaatg gactaggctt aactattctt tttattccac ttctttattt
    15901 tcaaaattga aaggctcttt catgatggct gagctgatag ggcgtttatc gaatttttga
    15961 ccgacagaaa ttagatggac taaattctgt tgataatttt ctgcaaaaac acaactatat
    16021 aacacgtcta gaagataaca aattgatgta ttgatcgtaa aattaccaat ttttgaatac
    16081 agtcgctcgc gtgtcgtaag tcgcaaagcg gctgtactca ttgcagatag cgttgaatca
    16141 ccgatactgg tcaaagctaa aattggaaca tgattttgtt tcaatatttc tgcggtacga
    16201 aggatcacat tgttttcacc cgtataagaa atcagaatcc cgcacatatc actcgtgcaa
    16261 ttgtacgctt catagaatga ttcaccaaaa gtggtgacaa tcgtgacatc cattttgatg
    16321 cgcttcattt tcagcatgaa atcttgagaa atcaaagtat tcgagttact ggcgaaaatc
    16381 acgatttttt ttgcttgaag cattagttgt ttggcttttt gcagttggtc atgatgaatg
    16441 agagaaagtg tatcatgaat cgtcatctgt tctaaagaag ccattttatt tgcgatcgtc
    16501 ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa attcccttca
    16561 agataggctg tttcagctaa aaaagcatta cgaaaactga cccagccatc aaaacccatt
    16621 ttttttgcta cacggatgag tgtagagggg tggacataac atcgctcagc aatcgcttgg
    16681 atggtcattt cgccaagtgc tggagaatcg cttaagagaa actggatcac ttcattttct
    16741 gaaggagaaa aggagatttc tttgattttt tcacgtaata acatgtagat accttcttct
    16801 tattttttta cgtaaattat aacttttttg aaaatgattt gtaaacggat ttatactata
    16861 taacttaaaa tagacatttt ttgacaagat atttacaaaa atccactaga aaacagtaaa
    16921 ttttgtaaat gttactggaa agaaacagat ttctattgtt tgttttgttt acaaaaaaga
    16981 taatgaaagt gtaaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
    17041 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
    17101 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    17161 gatcgttagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaagatcta
    17221 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    17281 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
    17341 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtcgt
    17401 tttggcaatc gttgaggaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc
    17461 gaacccaagt gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    17521 tggattatgc aatgtgccag tgatggcgat gatgacagaa ccagcaatgc ttggcaagaa
    17581 aaaagaagag ctgatctaca agtttgccgg tctaaatcat ttccattggc acaaagtagc
    17641 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagagaa
    17701 tggattaccg aaaaatatct ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa
    17761 catgatccct tgtggctacc accgctatta ctatcgtgaa gaagaaatgc tggaacatgc
    17821 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    17881 gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat tgcaaaaacg
    17941 aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct atggaaataa
    18001 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc tgccggcaga
    18061 ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg tagcgtttgg
    18121 ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    18181 gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta cgatcaatcc
    18241 acttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacacca
    18301 gcagtattta ccgcaattca aggagacaat cagccgatta aaagaagaag gaatcgaagt
    18361 tcaagatgaa ctggcaagag aattatcagg taatacacat ataatggagg aagtataatg
    18421 ggattcagaa aagatttttt atggggcggt gctacagcag ccaatcaatg tgagggcggt
    18481 tatgatgaag gtggacgcgg actagcaaac gtagatgttg tgccaatcgg aaaagaccgt
    18541 tttcctgtta tcaccggaaa gaaaaaaatg tttgaatttg atgaggatca tttttatcca
    18601 gcaaaaattg caatcgatat gtatcatcgt tataaagaag atattgcttt gtttgcagaa
    18661 atggggttca agacttatcg aatgtccatc gcctggagtc gtatttttcc aatgggggat
    18721 gagaaagagc cgaatgaagc tggcttgaaa ttttatgagg atgcctttaa agaatgccaa
    18781 aagcacggaa ttgagccact tgtgacgatt acgcattttg attgtccaat gcatttgatc
    18841 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgtcga
    18901 gtcatcttca atcgctataa aggacttgtc aaatattggc tgacattcaa tgagatcaat
    18961 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaaaaagg agaaaatgaa
    19021 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    19081 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    19141 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
    19201 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    19261 gagcgaaatg gcattcagat cgaaatggaa ccaggggatg ccgcattgct gaaagaacat
    19321 actgttgatt ttatctcttt ttcttattat gcttcccgag tttcgactac cgacaacgaa
    19381 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctgcaagca
    19441 agtgaatggg ggtggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    19501 gatcgttatc aaaaaccatt gtttattgta gaaaacggct taggtgcagt cgatacgcca
    19561 gatgaaaatg gttatgtgga agatgattac cgaatcgatt acctggcagc gcatatccag
    19621 gcgatgaagg atgcagtaga actagacgga gtcgatctat taggctatac gacttgggga
    19681 tgtattgatt tagtatcagc tggtaccggt gagatgaaga aaaggtacgg attcatttat
    19741 gtcgatcgtg ataaccaagg aaatggcaca ttagcccgtt caaagaaaaa atcattcaac
    19801 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaacgattg atcaatgatt
    19861 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    19921 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    19981 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
    20041 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggtgcccct
    20101 ggcggccaaa tgaacaatac ggcagaaatc gaaaattatc ctggattcga ttcgatcatg
    20161 ggccccgatc tagcttataa gatgtatgaa agtgtctcaa gatttgggac agaaaatgct
    20221 tatggaatcg ttcaagatat caaggatcac ggcagccata aagaagtcat ctgtgaagat
    20281 aaaagttacg aaggaaaaac agtcattatc gcaacaggat gtatccaccg taaattaggc
    20341 gtacctggcg aggaagaatt tgctggacga ggcgtttctt actgtgcagt ctgtgatggt
    20401 gcgtttttcc gtaacaaacg tctagtagtc atcgggggtg gggactcagc agtagaagaa
    20461 gccatctatt tgacacaatt tgcttctgaa gtagtgatcg tccaccgtag agatgaatta
    20521 cgtgcacaaa agatcatcca agaccgtgca tttgcaaatg aaaaaatttc ctttatgtgg
    20581 gatacagtcg ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt
    20641 aatgtcaaaa ccggcaatga atttgattta gcagcagacg gagtattcat ctatgttgga
    20701 ctggatccat taactgcacc ttttagagaa gcaggtttga cgaacgaagc tggctggatt
    20761 ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt
    20821 gaaaaagatt tgcgacaaat cactacagct gttggtgacg gcggtattgc tggacaacaa
    20881 gtgtataaat acttggagtc tttagaagaa acaacagcat aagtttaaaa agagtagaag
    20941 aacgggttca attgttcttt tactcttttt tttcgcgtga aatttactga atctgattta
    21001 tgcgaacacg tccaacacct tttgataaaa gaatcctttc ttgatttaag cgttttttgg
    21061 tcctcatact ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
    21121 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
    21181 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
    21241 gatgcttttt atgcaccgtt ggaattcggt actgcaggta tgcggggaat cttaggacct
    21301 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact agcacgtttc
    21361 atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
    21421 catcagtcac cagaatttgc aatggaagca gccaaaacac tagcacatca caatatcccg
    21481 tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg
    21541 aagacgtttg cgggaatcat ggtaaccgca tctcacaacc cagcggctta caatggatat
    21601 aaagtctacg gagaagatgg cggacaaatg cctcctgcag acgcagatgc attgacgaaa
    21661 tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga agaagctaaa
    21721 catagcgggt tgatcacgat catcggagaa gaagtggacg ctgcttatct agaagaaatc
    21781 aaagcagtga cgattgatcg tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg
    21841 tacacgccac ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga
    21901 ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag atccagattt cactacggtc
    21961 aaatcgccaa atccagaaga acattcagct tttgaatatg ctatacgact tggagaaaaa
    22021 gaaggagctg acttgctgat tgcaacagat cctgatgcag accgtttagg cgcagcggtt
    22081 cgtttgccag atggttctta tgaagtattg actgggaacc aaatcggagc attgtttgct
    22141 caatatattt tggaagcaca tcaatcaaga ggcactttac cagaaaacgc agcggtattg
    22201 aaatcaatcg tttccagcga attgcctaca gcaattgcgg aacactacgg tgctaccatg
    22261 ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa tccaacaatt tgaagaagac
    22321 cattcacata cttttatgtt tggattcgaa gaaagttatg gttatttagt taaacctttt
    22381 gttcgcgata aagacgcgat ccaagcattg cttttattag cagaagtagc agcttactat
    22441 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctat
    22501 ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
    22561 gctttgatgg aaaaattccg taaagaagca ccgacagaat ttgccggagc aaaagttatt
    22621 caaacagaag actttaaaga attgacgaga acagaccgtg aaggaaacgt agaaaaacta
    22681 acaacaccgc catccgacgt attgaagtat acattggaag aaaatggctg gatcgctatc
    22741 cgcccttcag gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg aagctctaca
    22801 gaagacgcag ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat gacagaagaa
    22861 taaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt
    22921 tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt agagaaagtc agtcaattat
    22981 tcaaaatgtt gagtgatccc actcgtctta aaattctgct atatctgaaa gacggggaac
    23041 aaaatgtgac agcaatcacc caagcagtgg aaatggagca gtctgctgta tcgcatcaac
    23101 tacgtttatt aagagaaaat catgtcgtca agtctcatcg cgagggaaaa gcgattttgt
    23161 atagtcttga tgattctcat gtactggata ttttgaacca gacactcaaa catgtggaac
    23221 atcaataacg ataggtgaaa atagataaac actggagtgt gacaacgaag aa
//
LOCUS       DADJYG010000028        23118 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.028, whole genome shotgun sequence.
ACCESSION   DADJYG010000028 DADJYG010000000
VERSION     DADJYG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.028"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..551)
                     /locus_tag="JZ382_000907"
     CDS             complement(<1..551)
                     /locus_tag="JZ382_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY2427110.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVTED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIRFSKKEAQIFHYYMQGVDFAEM
                     AEWLSMDHKQVMSAYGRVKLKIK"
     gene            complement(628..1158)
                     /locus_tag="JZ382_000908"
     CDS             complement(628..1158)
                     /locus_tag="JZ382_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAY2427111.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGLEVIVVFDAQLVPGIQQNYRKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFVDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1162..2022)
                     /gene="rlmB"
                     /locus_tag="JZ382_000909"
     CDS             complement(1162..2022)
                     /gene="rlmB"
                     /locus_tag="JZ382_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY2427112.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYPNKKRTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEAPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEYVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2015..2410)
                     /locus_tag="JZ382_000910"
     CDS             complement(2015..2410)
                     /locus_tag="JZ382_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HAY2427113.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLINQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2407..3819)
                     /locus_tag="JZ382_000911"
     CDS             complement(2407..3819)
                     /locus_tag="JZ382_000911"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY2427114.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIQNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKITLLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQAEVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFARSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3816..4352)
                     /gene="cysE"
                     /locus_tag="JZ382_000912"
     CDS             complement(3816..4352)
                     /gene="cysE"
                     /locus_tag="JZ382_000912"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY2427115.1"
                     /translation="MSWFARAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4672..6132)
                     /locus_tag="JZ382_000913"
     CDS             complement(4672..6132)
                     /locus_tag="JZ382_000913"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY2427116.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMDWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDVVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6302..7459)
                     /locus_tag="JZ382_000914"
     CDS             complement(6302..7459)
                     /locus_tag="JZ382_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAY2427117.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7493..8863)
                     /gene="radA"
                     /locus_tag="JZ382_000915"
     CDS             complement(7493..8863)
                     /gene="radA"
                     /locus_tag="JZ382_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY2427118.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRVKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFH"
     gene            complement(8926..9453)
                     /locus_tag="JZ382_000916"
     CDS             complement(8926..9453)
                     /locus_tag="JZ382_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY2427119.1"
                     /translation="MRKRGFEVISKYKEQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETIADALKKDVSENEKQLKPILVPTGVKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9683..10489
                     /gene="proB"
                     /locus_tag="JZ382_000917"
     CDS             9683..10489
                     /gene="proB"
                     /locus_tag="JZ382_000917"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY2427120.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10486..11733
                     /locus_tag="JZ382_000918"
     CDS             10486..11733
                     /locus_tag="JZ382_000918"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY2427121.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11809..12153
                     /locus_tag="JZ382_000919"
     CDS             11809..12153
                     /locus_tag="JZ382_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427122.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12270..12596)
                     /locus_tag="JZ382_000920"
     CDS             complement(12270..12596)
                     /locus_tag="JZ382_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427123.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13211..14413)
                     /locus_tag="JZ382_000921"
     CDS             complement(13211..14413)
                     /locus_tag="JZ382_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2427124.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14530..15045
                     /locus_tag="JZ382_000922"
     CDS             14530..15045
                     /locus_tag="JZ382_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY2427125.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15244..15690)
                     /locus_tag="JZ382_000923"
     CDS             complement(15244..15690)
                     /locus_tag="JZ382_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAY2427126.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15737..17215)
                     /gene="msr"
                     /locus_tag="JZ382_000924"
     CDS             complement(15737..17215)
                     /gene="msr"
                     /locus_tag="JZ382_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAY2427127.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17569..18198)
                     /locus_tag="JZ382_000925"
     CDS             complement(17569..18198)
                     /locus_tag="JZ382_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAY2427128.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18182..18646)
                     /locus_tag="JZ382_000926"
     CDS             complement(18182..18646)
                     /locus_tag="JZ382_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2427129.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19133..20401)
                     /locus_tag="JZ382_000927"
     CDS             complement(19133..20401)
                     /locus_tag="JZ382_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAY2427130.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20632..21369)
                     /locus_tag="JZ382_000928"
     CDS             complement(20632..21369)
                     /locus_tag="JZ382_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2427131.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21436..22005)
                     /locus_tag="JZ382_000929"
     CDS             complement(21436..22005)
                     /locus_tag="JZ382_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAY2427132.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22133..23005
                     /locus_tag="JZ382_000930"
     CDS             22133..23005
                     /locus_tag="JZ382_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2427133.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         6478 a         4982 c         4193 g         7465 t
ORIGIN      
        1 ttttttattt tgagttttac ccttccatat gcactcatca cttgcttgtg atccattgac
       61 aaccattcag ccatctccgc aaaatcaacc ccttgcatgt aataatgaaa aatttgtgct
      121 tctttttttg aaaatcggat ctcgcaattt tcgattgctt cttttaacaa aagatgctga
      181 atcgctaaag gttcctgcaa ggtaatctct gctaaaaaat ctggtccttc tgtctctatt
      241 ttttggtcga tcgagacttc atctttttgg tcgcggcgct tcagcgcgtt ctgttttctc
      301 aataaagaat aaatttgatt gcttataata cttttataat aacttccaaa gctaactcct
      361 ttgctttttt cgtaagttct caatgcctga tataaagcga tcctcgcttc ttgttcccaa
      421 tcctcagtaa cataatttct taaataataa gtgtttttca aacgtagtaa gagtggaaaa
      481 tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc gcgcaataac
      541 tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt atcatcaatg
      601 attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat cgtccaattt
      661 ttgggacaat ttttttaact gctcttcatt ccacggagaa ttacgtcgga aatcaacaaa
      721 atgcatatct ttattttctt gggctattgt tttttccgtt tttttaatcg ttttatataa
      781 ttcataagca gaagtccgca acgccccctg agaaaaaatc atccactgtt ctgctagatc
      841 gcttgtcgcc actgttactt gtgtcaaacg atcattcagc tgcccagcga ttctttcgat
      901 ataactgtca gccgtttcat cttctttcgt aaaaacaaca tccaactgat atttcctata
      961 attttgctgg atgcctggta ctaattgagc atcgaagaca acgatgactt ccagtccttc
     1021 atatttcgca taatttgata aacgatgcaa aagagcttct cttgcatctg ccattttgtc
     1081 ctgcttttta agctggacca gttccggcca cgaaccgatc atgttgtagc catctacaat
     1141 caacagctgt tttttcatga cctactcccc ctgacgttgg gaaaaagcct gatacatcag
     1201 caatccagcg gcaacactgg catttaaact ttggacgtgc ccagtcattg gaatcgtcag
     1261 taattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat taccaatgac
     1321 taaagcaatg gatcctttgg cattccattt ttggaagttt gtgccggtca tatctgtgcc
     1381 aaatacccaa aatcctgtct ctttgagctg ttgcacagct tgtaccaaat tcgtcacacg
     1441 tgcaacaggc acatattcca ctgcaccagt ggaagtcttt gttacgatag gcgtgattcc
     1501 aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg ctgtccgtaa
     1561 aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga agggggcttc
     1621 ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat aaggtgtaat
     1681 cgcaagaacg actccttgat gcacgccatg atcgctcaaa gtttctaact tttgtttagg
     1741 gacccatttg accggtacag cttgctcacg tgcgatttct ttcagttctt ctactttttc
     1801 tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta gcgcttctac
     1861 tgcggcgtgg tgtccaaaga cgaaattatc ttctaatgct tcttcgtttt gatcagaaac
     1921 tgactgattt cgtccagttc gctttttatt tggatatcta ttttgttttt tgtcttcaaa
     1981 tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca ccaactttct
     2041 gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa tgcagatagc
     2101 ccatcagtgc ttcgaatcct gttgcgaccc ggtaagtcgt aacatcagca ttttttgcac
     2161 tcgtatggct tttggcattt cttcctctgc gataataaag ctcttcttct tctgtcaaga
     2221 gatgttcctc cagcatttgc tgaatcaaaa aagcttgtgc atttgcagaa acataattcg
     2281 ttgccgcacg atgcaactta ttgggttttg tttctccttg attgatcagg tggtcacgaa
     2341 catagatttc gtaaatcgca tctcccacgt aagccagggc taaaccgttc aattgtgtgt
     2401 aatctctcat gcttctcttc tccatcgggt tccttggggt gtatcttcta ataagatccc
     2461 tttttctttc aacaaatcac ggatttcgtc gcttcgggca aaatcacgat tgcttcttgc
     2521 ttgatttcgt tcttcgatca attgttcaat ctcttcatcc aataattctt ctgctgtaaa
     2581 taaaataccg aagatcgaaa gccattgcga gaataaagct tctccctgtg ccaagacagc
     2641 ggcagaaact tccgcttggt ctagataagt gttcagccat ttagcaagct catagaccac
     2701 tgtgatccca tttgctgcat taaaatcatc atccatttct tcgatgaatc gctgttccaa
     2761 attttctaac aaagtgattt tatccgcatc atctgccaaa ttctctgagg cagtttcttg
     2821 gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg ctgcttcttt
     2881 catcgtcgtt tcactgtaac ggatcggacg acggtattgt gtggtcgcca tgaagaaacg
     2941 caagacttgt ggatcaacgt tttggatcat ttcatgtaca gtgatgaaat tccctagaga
     3001 tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca tccagtaatt
     3061 agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcgt gatgaggaaa
     3121 ttccaaatct tgcccaccac catggatatc gattgtttcg cctaaatgtt ttgtcgccat
     3181 gacggaacat tcgatgtgcc aaccgggacg ccctttgccc catggtgaat cccaagaaat
     3241 ctcgcctggt tttgcttgtt tccacaaagc gaaatctaat gggtcttctt tttgctgctg
     3301 ttcttcacca gtacgctgac ttgcgccgac ttccaattca tcgatggatt ggtcacttaa
     3361 tttcccatag ttttcaaatt tacgggtacg gtaatacaca tcacctgaag attcataagc
     3421 atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat ggtccatgac
     3481 tcttggatga agtgtagcag gctcgacatt caacgctttc gtatcttctt caaatgctcg
     3541 gatgaaacga tcagcgactt ctggtgctgt gatgcctaat tcgtttgctg tacggatgat
     3601 tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc gatattcaaa
     3661 atagcgtcga atcgtatcga aagcaatggt gctacgagca tttccaatat ggatataatt
     3721 atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag ggatgaattc
     3781 ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct cactttttct
     3841 cctttgattc gaacgacttt tgctggtact cctactgctg tagcgtcgtc tggaacatct
     3901 ttcaacacaa cggcacctgc gccgattttt gcgttttttc cgatcgttac cggtcctaat
     3961 atctgcgcat tcgcagagag catggctcct tgtttgaccg ttgggtgtct tttacctgtt
     4021 tcttttcccg taccgcctaa tgttacgcca tggaacaaaa tcacatcatc ctcgatttct
     4081 gcggtttcgc caatgacgat ccccattcca tggtcgatga acacacctgt tccaatcgtt
     4141 gctcctggat ggatttcaac acctgtcagg aaacgccaaa attgagcatt gatcttcgcc
     4201 aataaaaaga gccgatgctg atataaaaaa tgagagaaac gatgccaaaa caaggcatga
     4261 aaaccaggat aggtcaaaac gatctccgct gtcgaacgtg cagccggatc gtttctcttt
     4321 gctgcatcaa tcgctcgggc aaaccaactc atgactttcc actcctttcg taagtatttt
     4381 atgtaggaaa acagcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     4441 gacgctttcc gcgtggttcc acttttcttc ataatcaacg attatctctg acagctaacg
     4501 cctgttatac gttcttggct acttcgtttc ctgtaaaaca gtttttcctg ttttctgtgt
     4561 tttcgccaag accactccca gaggcatttc ggcaactatt cattgacacc ttgcaccacc
     4621 cggcatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag
     4681 tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa
     4741 ttctgggcca tgcatttgtc ctgatacagc tacacggatc ggcatgaaaa gatttttccc
     4801 ttttacgcca gtttcttttt gaacagcttt gatagcagct tttacagttg gagcatctac
     4861 tacttccatt tgttctagct gtgctttaaa tgcgtggaga acagtaggaa ctgtttcacc
     4921 agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga
     4981 cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt
     5041 cacccattcg attgtctttt catctgggtt ttctgctaca cgtccgtcag cgattagata
     5101 aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac
     5161 ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa
     5221 cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa
     5281 taatgcaata aagttgaaca ttgcatctgg tagataacct aattcgcgat attgttcgat
     5341 aaattgtaag attgattcgt cacgtttgct tagttttttg cctgtttcac tgttgatgat
     5401 caacgtcata tgtccgaatg ttggtggcgt ccagccaaat gcttcataga tcatcagttg
     5461 ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg
     5521 gtcatcgacc gcaacagcaa agttataggt tggcatacca tcgcgttttt ggatgacaaa
     5581 gtcgccgccg atattatcgg attcgaacga aatagcgcct tttaccacat ctgtaaatgc
     5641 gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt
     5701 ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc
     5761 tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta
     5821 tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga
     5881 ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat ccattcccaa
     5941 ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt
     6001 atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaacaa
     6061 tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg
     6121 tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag taaaaattcc
     6181 gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt
     6241 tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt
     6301 tttattttga ttgttttgtc tttttctctg tctctttttt attgtcgatc cctcgtcctg
     6361 catgggcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagagcg cttgtcacaa
     6421 caacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccat
     6481 catcaagata ggctacccct tgttggcgtt ctgttccggc ttttactact agaacattca
     6541 tcgtttcgcc aggaatgacg actggtttca cggcatttgc caacgcattg atattaagaa
     6601 caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt acgatgacac
     6661 cgtctaaaag cttggctaat ttgatcaact tgctgtctac ttcgctgata tcttcaaagt
     6721 ctccatcgta catttcgaca gatatacctt cttctttttg caaagcattt aggatatcta
     6781 atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt
     6841 cgtaaagaac aaaattaggg atcatcaagg ttccttctaa aaatccagtc tttgtaatat
     6901 cataaatccg accatcaata atcacacttg tatccaagat tttatattta tggaaatttt
     6961 ctcctgattt tcgttctaac agttggctct ccacttggct agctgtttct tgctcgattt
     7021 ttttgcttcg tgaaccaaag actttcttcc attcttcgat tcgtgtcgtt cccattctca
     7081 atcccaaata acctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa
     7141 tttccatatt gtaaagaggg gttgagatca caacgcctaa agtcaatccg atgatcgcac
     7201 cgatcgcacc gaagagaaga taagtcaagc tgacctcgct tagtttttgt tctacttgtt
     7261 tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc
     7321 ctaacaaact gttggtaaat gtattattca gccacccatt tgactgttga ccgattgcct
     7381 gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaacg
     7441 taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt atttagtgga
     7501 acacacgctt caacgtttca gccaaagttg ctactccaac gatctcgatt ccttttggcg
     7561 cttcccagtt gcctaagttg tttttaggca ggtaaacttt tgtgaagccg agtttttgga
     7621 cttctttgac ccgttgttca atatgactga cacgtctgat ttctccagtc agtccgattt
     7681 caccgatgaa acattccgtc ggctgtgttc ctttttcttt gtagcttgaa gcaatactga
     7741 cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt
     7801 aagcatcttg attctgaaga agtagtccag ctcttttttc cagcactgcc atgattagag
     7861 atacacgatt gaaatcaaga cctgttgttg ttcgcttagc atttccgaac atagtaggtg
     7921 tgactaatgc ttggatctct accaaaattg ggcgtgttcc ttccattgct acaacgatag
     7981 cagagccagt cgcaccgtct aggcgttcct ccaagaaaat ctgtgaaggg ttagcgactt
     8041 cttccaatcc atgctcatgc atttcaaaaa tcccaatctc gttcgttgag ccaaatcggt
     8101 tttttaccgc tcgtaaaatt cggaaactat gatgttgttc accttcaaaa tataacactg
     8161 tatccaccat atgctctaac attcttggac cggcgatcga accttctttc gtaacatgac
     8221 cgacgataaa aatggcaatt ccattcgttt tagcgatttt gagtaattct gcagtggttt
     8281 ctcttacttg gctgacgctt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga
     8341 tggaatcaat aataacataa tcaggggaga tatgttcgat ggcacgactg atttcattca
     8401 tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgc tccgcgcgta
     8461 attttatctg ttctgcgctt tcttcgccgg atacatacaa gactttcccg cctatggctg
     8521 ctaattgttg agaaacttgt aacagcaaag tggatttacc aatacctgga tctccaccaa
     8581 tcaagaccat ggatcccgga accacgcctc cacctagcac acggttcagt tcttctaatt
     8641 tcgttttgac ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggtttcgctt
     8701 tttctcctgt caaacttgtc cgcgttctgc ggtctgttgt atcttgttcg atttcttcga
     8761 ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc
     8821 cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctctta
     8881 ttctaaccat gagacattaa atcaagctta aatttcttta tcgttttatt tactggaaga
     8941 accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaatggcaa
     9001 gaagatccct tgaccgatac gctcaccttt cttgatcact acatccttga aaccaaagtt
     9061 ggtgaattgg aacatgatat gtccttcatt tgcctcatta ttgtaataat cactgtcaat
     9121 cacacctacg ccgttcgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc
     9181 aagctgcaaa aattcatctt ctcccatgta ggctttgacc ccagtaggca caaggatcgg
     9241 ttttaactgt ttttcatttt ctgatacgtc ttttttcaat gcatctgcga ttgtttccaa
     9301 tccttttttc cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa
     9361 atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gctctttata
     9421 tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc
     9481 ctattctaac atattcgagg ctataaaaga ctctctttac aaattcttca agaaattctg
     9541 ctatcctaaa gtcaaccatt aacgcgttct tttaccgaac aactgcaagt tgtacaaaaa
     9601 aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt
     9661 ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt
     9721 caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga
     9781 tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc
     9841 atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat
     9901 tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca
     9961 aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga
    10021 ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac
    10081 gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa
    10141 atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt
    10201 gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc
    10261 tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc
    10321 tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt
    10381 tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat
    10441 tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg
    10501 caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc
    10561 aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt
    10621 gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa
    10681 gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt
    10741 gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg
    10801 agaatcggca aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca
    10861 aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc
    10921 ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg
    10981 gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact
    11041 gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca
    11101 aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga
    11161 aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc
    11221 aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct
    11281 gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct
    11341 ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca
    11401 gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat
    11461 gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat
    11521 agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat
    11581 gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct
    11641 actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat
    11701 atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc
    11761 ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt
    11821 ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt
    11881 attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg
    11941 tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac
    12001 atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc
    12061 gcttatacga ttacgctact gattgctggg atttttcact tatcttataa atatcagctg
    12121 aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc
    12181 cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca
    12241 gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct
    12301 tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc
    12361 atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc
    12421 cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc
    12481 gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat
    12541 tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat
    12601 tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt
    12661 cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa
    12721 taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga
    12781 aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt
    12841 ggtaaacggc gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag
    12901 aagctatttt tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc
    12961 tgttaaacgg tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga
    13021 ggaactattt ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc
    13081 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    13141 tgagaagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
    13201 ctctgatttc ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac
    13261 atacactgat aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt
    13321 caccaatcac acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat
    13381 agctggacga agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca
    13441 gtgtattgat aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg
    13501 gcatagaagt tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta
    13561 aaattgtccg ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa
    13621 tagccagaag cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa
    13681 tcaataaaaa tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga
    13741 ttagtccaac tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta
    13801 cttttctctt tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa
    13861 acatcaaaaa tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac
    13921 cgactagagg gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt
    13981 tttctgtgtt accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag
    14041 cattcccgac accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc
    14101 cgccaagacc agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac
    14161 cgatccgact agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac
    14221 tagtaaataa cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta
    14281 agattgggtc tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta
    14341 gcgctgaact tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct
    14401 ccaatatttc catccctttc tctctattag catcatacat gatttatttt acatgtcaat
    14461 atgcacatgt aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt
    14521 aggattttta tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt
    14581 tctgggtatg aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc
    14641 tatacaacgc ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag
    14701 cactctcaaa aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg
    14761 atcaaagagg aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct
    14821 ttttcgacgc tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg
    14881 aacaaaatca tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa
    14941 gaatttggac gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa
    15001 tggtgtcagc aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc
    15061 tttctagaaa tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa
    15121 aagaaaaagc gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga
    15181 actgcattca agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt
    15241 ccatcattca ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat
    15301 caactgcaac ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa
    15361 taattgatca ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat
    15421 tcctaaaacc atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac
    15481 tggtagttta aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa
    15541 agccaccacg aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg
    15601 cagccataaa ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa
    15661 aattattcct tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa
    15721 aatcatcgta gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta
    15781 tttcataaac ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa
    15841 tcgttcccgg atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa
    15901 aattagtcgg ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg
    15961 tcagacgggt cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag
    16021 caaaccctaa gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca
    16081 tgacaaaacg aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt
    16141 gaaagcttac tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata
    16201 aggttgtctt acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact
    16261 gaaaatcacc atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca
    16321 tgatgggata gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca
    16381 tctgctgcgg cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat
    16441 tcttttggat ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc
    16501 tgatttgttg ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct
    16561 tttcttggat cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta
    16621 gctgagtctt tttctccaat tccttttgag tgagataggc actataattc cccgaaaact
    16681 ctcggatttc tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc
    16741 gatcatgact aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat
    16801 caatactttt tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag
    16861 aagaaagaag acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg
    16921 aagtttcttc cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt
    16981 cagcgagata ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat
    17041 gatcgggaaa aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt
    17101 ctcctatgat ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta
    17161 gctggttgcc aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt
    17221 ttacactcct atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct
    17281 gtttgcaaaa aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat
    17341 caccaaatct gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc
    17401 atccatgaaa ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt
    17461 cgaaacgtaa tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac
    17521 acacgattca aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat
    17581 agtgatcttt tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat
    17641 tgataaaata ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg
    17701 atctgcttcg aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat
    17761 gaattgcata gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga
    17821 aaaactcttg taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat
    17881 cttccctgat tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc
    17941 gccaaaaatc atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa
    18001 aggacgcaag atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat
    18061 attgacagaa gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg
    18121 gattttgtat gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa
    18181 atcatttttt tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt
    18241 gggtattcaa tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg
    18301 gggatagcac ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg
    18361 ctttttctgt tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa
    18421 catagccatt tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca
    18481 acttttcttg caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct
    18541 cgtatctagt aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg
    18601 ctaatttgat ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc
    18661 taataattga ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca
    18721 aagaatcagc tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt
    18781 ttttgtagaa tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg
    18841 attcttatca aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa
    18901 tttccgaaat cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt
    18961 aaacagtaat aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt
    19021 tcaaaaactt gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt
    19081 tgtttggctg ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac
    19141 cataaaatcc tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag
    19201 cactcaaggt atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg
    19261 gcttggaacc accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag
    19321 agttgatctc agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac
    19381 ggtagacagg ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta
    19441 cagttggcag ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac
    19501 ggttatacag acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat
    19561 tataggtttc gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac
    19621 ctgttagcca agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat
    19681 agtagtaaac gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg
    19741 attctttgta cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca
    19801 catattccga agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata
    19861 gattgtaatt aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt
    19921 gagcaatcat gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg
    19981 caatactatc aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc
    20041 cagtatagtt ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat
    20101 ctgtcaattg gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg
    20161 tactgtcttc ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg
    20221 tattttctga gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt
    20281 ctgaagaact cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg
    20341 tgttggcaac tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca
    20401 tcttttttct cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa
    20461 aagcaagtca atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc
    20521 tgcataaaaa gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg
    20581 tttgaataac tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc
    20641 ctgtgtcgtc ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc
    20701 aaatgctaca gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg
    20761 aagttcaaga gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc
    20821 tggagagatg atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat
    20881 gattgcacgg acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg
    20941 tcccgccaca gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag
    21001 tacggcggaa attccattta acacacccat gatgttgata tcaagcatat tttgccattc
    21061 atcacggtga ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac
    21121 atcgattcga ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc
    21181 tcttacatct gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt
    21241 taggcgatct tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt
    21301 cgttgttgct tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa
    21361 atcattcatt ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt
    21421 atttcatctt actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc
    21481 tgcaacgatg gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc
    21541 attctcgcca atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt
    21601 cttgatgtgg attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt
    21661 caccgtgatg aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc
    21721 taagtcaacg aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt
    21781 gccgaaatct gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat
    21841 gatattttct agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc
    21901 taaaggctct tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac
    21961 tcgaatctct ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt
    22021 tatccttact ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta
    22081 ttccttttaa ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat
    22141 tcgtatactg acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa
    22201 atctctgcat atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact
    22261 tggtgtgaca cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata
    22321 tctttattca aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac
    22381 aaatgacgca atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc
    22441 tttcatttct gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt
    22501 atatagcggg aatgcagatg atatcataga caaactggat agcggattac ttgattttgg
    22561 actcgtaatc gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga
    22621 tcgttgggga cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc
    22681 gcaggaactg aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact
    22741 agctgaatgg ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt
    22801 gtacaatgct tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat
    22861 catcaatact tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc
    22921 actgaatctc atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga
    22981 tgtcctaacg gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac
    23041 aacgaataag ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc
    23101 ttccttttat ttctgata
//
LOCUS       DADJYG010000029        22773 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.029, whole genome shotgun sequence.
ACCESSION   DADJYG010000029 DADJYG010000000
VERSION     DADJYG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.029"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            58..468
                     /locus_tag="JZ382_000931"
     CDS             58..468
                     /locus_tag="JZ382_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY2427134.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            461..1162
                     /locus_tag="JZ382_000932"
     CDS             461..1162
                     /locus_tag="JZ382_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAY2427135.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            1162..2748
                     /locus_tag="JZ382_000933"
     CDS             1162..2748
                     /locus_tag="JZ382_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAY2427136.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2767..2988
                     /locus_tag="JZ382_000934"
     CDS             2767..2988
                     /locus_tag="JZ382_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAY2427137.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2985..3746
                     /locus_tag="JZ382_000935"
     CDS             2985..3746
                     /locus_tag="JZ382_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAY2427138.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            4126..4635
                     /locus_tag="JZ382_000936"
     CDS             4126..4635
                     /locus_tag="JZ382_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAY2427139.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4710..5249)
                     /locus_tag="JZ382_000937"
     CDS             complement(4710..5249)
                     /locus_tag="JZ382_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAY2427140.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5389..6000)
                     /gene="rpsD"
                     /locus_tag="JZ382_000938"
     CDS             complement(5389..6000)
                     /gene="rpsD"
                     /locus_tag="JZ382_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY2427141.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6316..7113
                     /locus_tag="JZ382_000939"
     CDS             6316..7113
                     /locus_tag="JZ382_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAY2427142.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            7141..7776
                     /locus_tag="JZ382_000940"
     CDS             7141..7776
                     /locus_tag="JZ382_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAY2427143.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7796..8569
                     /locus_tag="JZ382_000941"
     CDS             7796..8569
                     /locus_tag="JZ382_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427144.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8977..9774
                     /locus_tag="JZ382_000942"
     CDS             8977..9774
                     /locus_tag="JZ382_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAY2427145.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9752..10165
                     /locus_tag="JZ382_000943"
     CDS             9752..10165
                     /locus_tag="JZ382_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427146.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            10149..12794
                     /gene="mutS"
                     /locus_tag="JZ382_000944"
     CDS             10149..12794
                     /gene="mutS"
                     /locus_tag="JZ382_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY2427147.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12812..14920
                     /gene="mutL"
                     /locus_tag="JZ382_000945"
     CDS             12812..14920
                     /gene="mutL"
                     /locus_tag="JZ382_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY2427148.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14942..15502
                     /locus_tag="JZ382_000946"
     CDS             14942..15502
                     /locus_tag="JZ382_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAY2427149.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15587..17575)
                     /gene="treP"
                     /locus_tag="JZ382_000947"
     CDS             complement(15587..17575)
                     /gene="treP"
                     /locus_tag="JZ382_000947"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAY2427150.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17798..18511
                     /gene="treR"
                     /locus_tag="JZ382_000948"
     CDS             17798..18511
                     /gene="treR"
                     /locus_tag="JZ382_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAY2427151.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18588..19034
                     /gene="msrB"
                     /locus_tag="JZ382_000949"
     CDS             18588..19034
                     /gene="msrB"
                     /locus_tag="JZ382_000949"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY2427152.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            19204..19806
                     /gene="ruvA"
                     /locus_tag="JZ382_000950"
     CDS             19204..19806
                     /gene="ruvA"
                     /locus_tag="JZ382_000950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY2427153.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19819..20820
                     /gene="ruvB"
                     /locus_tag="JZ382_000951"
     CDS             19819..20820
                     /gene="ruvB"
                     /locus_tag="JZ382_000951"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY2427154.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20849..21427
                     /locus_tag="JZ382_000952"
     CDS             20849..21427
                     /locus_tag="JZ382_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAY2427155.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21479..21961
                     /locus_tag="JZ382_000953"
     CDS             21479..21961
                     /locus_tag="JZ382_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAY2427156.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            22078..22452
                     /locus_tag="JZ382_000954"
     CDS             22078..22452
                     /locus_tag="JZ382_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427157.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7410 a         4071 c         4926 g         6366 t
ORIGIN      
        1 aactttcaga aaaggtttgt tagtaaaatg aaagctatac gtaaggagtg ttgtttgatg
       61 aaattttttc aagagttttt cattattatt ttcttttctg tattaggaga aggcatacgg
      121 atttttgctc atttacctat tcctggaagt atcttaggta ttcttttttt atttttagca
      181 tttgaaatgc atcttgttga tcctgaaaaa atcggagcaa ctggtgattt tttattgaat
      241 aatctagcca ttttatttgt tcctgctggt gtaggtttat tggaatattt tgatgatatt
      301 gcttcgatat ggcctgtttt actgggagca gtcgtcgtgt gttccatcgt cacgatggtt
      361 gcagcgggaa aaacggcaga aggtgtagaa gctttgctgg gctatgtccg taagaaaaaa
      421 gcagaaaggg tatctgtaaa cgaaatggag gagaatcaaa ttgactgagt tttttgtgaa
      481 tccattattt ggcttaagtt tgactttagg agtgtacgta ttatctgaca aatttttgcg
      541 gaaatataaa ctgcctgttt tgaatcctct cgtattatcg attgttgtta ttatcttatt
      601 tttgcatttt tctgggatca gctataaaga ctataatatc gggggcaatt ttttgactat
      661 gctgattact cctgcgacag tggcacttgc catccctctt tataaaaact tccgtttact
      721 gaaagagaat ttttatcctg taatggctgc gattttagca ggaatagtcg caaattgctt
      781 ggttagtgta gggttcggtt acttcttctc tcttcataaa gagatggtga tctcattact
      841 acctaaatct gtaacgacgg ctatttctgt cgatttatct catacgatgg gcggggtgaa
      901 tgccgtgaca cttgctattg ttgtctctac aggtattttt ggttcgctga tcgccactca
      961 tattttccgc ctattcaaga tcgaaagtcc agttgctcgc ggtgtggcgt taggaagtac
     1021 ttctcatgct attggtacag caaaagcaat cgagattggt gaaatagaag gaatcatttc
     1081 cggacttgcg atatgcgtca acggaatatt gaccgtttta ttgctaccgt tattctttca
     1141 gccttttgct ggcttgtttt gatgcgaaaa ccactatttt atgctacaat aaccaaaacg
     1201 ttcgagcata aaaaaggagg gaaagacgtg tatttgtctt ccgagtatct taaacaattt
     1261 gataaagatc tatattataa gattctcctc ctagaaagtt ttgaggatca agcttggcat
     1321 accgctgcac aattagcgca agttgttcag ctggatgccc gcagcgtgtc gaaatacctg
     1381 aacgagctgt ctaaaaacta tcagcagttt agcggtaaga cacacccttt gtttacaaaa
     1441 aatcaccgtt ctggttataa cttttatgat acgctcgatt caattgaaca tgagcgtttt
     1501 ttgatctatc tcgtgcaaag tacgctgaaa ttccagttgc tgcacgacat tttttttgaa
     1561 gaattccata caatgtacca atttgcgcaa aagcattaca ttagtgaatc tactgctcat
     1621 cgaaaaatca atgaatggaa acagcaactg caaacttacg gaattcggct gcaacgaggt
     1681 acatatatcg ctcaaggcga agaagaaatc attcgacttt acttgcatat gacattctgg
     1741 cagttgtttc gagggaaaat ctggccattt gaaacgattt ctcaaatgga cgtaaagaat
     1801 atggcagaac atatcatggc tttttttaat gttcgtctga acgagatcaa aaaaagacga
     1861 ttggaatata tgctaggtgc ctttttttta agaaaatcac agaagcatta tgttgtattg
     1921 aatgagaaaa aaagacggct gatttctgac aatctgttgt tccagcgatt ttgccaggtc
     1981 atggagccgg tatttcctaa ttattttcaa gtagaagacg agttaggcgc attatttctt
     2041 gtactgatga ctcgtgaaga atattatagt gatcctaaga tacggaagaa aatttttgat
     2101 tttcatcaac aagcaaaaac accgcccttt acagcccttt cagaagcaaa agccgctctt
     2161 tctctttatc aggaagagca ggggctgcca gcagaaaatc taaccttcga ggcagaaaac
     2221 tatttattca gcagtcattt tttcgcctat ctttttccta atgcgaaaga aacgattgat
     2281 ggcaatagta gtgattttat taatcattta gttattgaaa acaaggaatt gaaacaatgg
     2341 ctcgtacatt tttttgaaag tagacacaaa catcccaatc atctggcatt taaaaatcat
     2401 gcgtttttga tggggcggta tctgactgtg tttaaaacat taggagcctt tactccacag
     2461 cttccaaaga tcacgatcct tttaatgaca gatttcccat tatttgaaga gcaactgtta
     2521 gaggagggat tacggacatt tttccgtaat gagtatcagc tgatttttct gccaacagat
     2581 tatcggggga gagaagttga tcttctgatt tcgacaagta aagtccaccg caaaccatgg
     2641 gcggatcttg actattttat tgtgactgaa gaactaaaat taattgatta catccaacta
     2701 tctcaaaaat ttgaaatgat tcaaaaacaa aaactattaa aacaataaca aagaaggaga
     2761 aaaaagatga catttgaaga aattttacca gaattgaaaa aaggtgcaaa gattattcgt
     2821 aaaggatgga gtggatttga actttatgtt gttctcgtat ccgatgatga atttgatggc
     2881 tgtccggtga cgccgtactt attgatcaaa acaagtgacg aaggtttttc cagcttcgct
     2941 cctacagttt gtgatatttt agcagatgat tgggcgatcg tagaatgaaa aaccagtatc
     3001 cagatctagc taaacaggtt gtatttatca caggagctgc ttctggaatt ggtgctgcgc
     3061 aagcccgagc gtttcttgaa caagatgctt ttgtatttgg attagatcaa aagttcggta
     3121 aaatggaaga aatttctttg cgatttcctg aaacttttgc ctacgcacta ggtgatgttc
     3181 gacaaatgaa agaactccaa caagcggtat cgaaatgcca aaatcatttt ggtgaagtta
     3241 cgattttact gaatacagcg gggatattgg atgcctacaa acccttgatg gaaactgacg
     3301 aatcgctgtg ggatctgatt tatgagacga atgtgaaaag catgtatcag ctgacaaaaa
     3361 tcatccttcc tgatatgatc cgccaaaaaa gcggaacgat catcaatatg gcttcgattg
     3421 ctggcttgat tgctggtgga ggtgggatcg cttatacaag cgcgaaacat gcgattgtcg
     3481 gatttaccaa acaactcgct ctggattacg caggacaggg tatccgagta aaaggaattg
     3541 ctccaggagc catccaaaca ccaatgaatg cggctgattt tgaagggaaa ggtgagatgg
     3601 cagagtgggt ggcaaatgaa acaccagtca agcgatgggc acagccggaa gaagtagctg
     3661 agctgacgtt atttcttgcc tctcctcaag caagctacat acaaggaacg atcatcccaa
     3721 tcgacggcgg atggttgtta aaataacctt gtgttttttt caagcttctg tatgataaaa
     3781 ctaaagagag aaacaacggt tgatgataaa aaaagtcgat gactgttccc gacattcgat
     3841 gccgaacagc cagttcaaaa aattcttttt aataatcgtt aatattcgaa actaagcggc
     3901 ttttcacgac ctcttgatat aaacgatatt caaaagcttg caactgcggt aacaagtcca
     3961 gccaaatgaa aaatataagg agctattttt atttgaccat tcttattact tgttgcaggg
     4021 cagctttttc actaccttct gcgacacttc agttggaatt ttcttcccat cctttggtcg
     4081 cggtcattac caagataaaa ttatcttgca aaggagaaat agaacatgga aaaaaataat
     4141 acccgttcag ccaaacgctg gacaacgaca gatgtgactc ggatggcgct agtcactggt
     4201 ttatatgtgg cagtgacgct ggttttgtct gtctttagct ttggagtgat tcagattcgt
     4261 ctctcggaga tgttcaacta tctgtccttg tataacaaaa gatacatcgt cgcggtaaca
     4321 gcgggagtag ctttggctaa tattgcttct cctcttggac tgatagatgt tgcagtagga
     4381 agcgtcagca cgctgcttgt attgcttatc aactataaaa tcactagtcg catcaaaaac
     4441 atgaaaatca aaatggccgt gacagcatgc gtatttgctt tttccatgtt tacagtagct
     4501 ggtcagctaa cgattttata tcaactacct tttttcttga attggtgggt gattgcttta
     4561 ggagaattgg catcaatgat tgtcggtggg ataattattt attggatcgg taaaaaagtg
     4621 gatttgtcaa aataaagatc tgtttaattt aaaatagggc agcatggaag agactgaatg
     4681 ttgagtcttc ttccatgctg cttttttgat taatttgtat gaagaaatcg atttagccgt
     4741 tttttcatca agataccgat ataagctgct ccagttgcct tgattgcttc cggcagtaaa
     4801 aacggcagta gtccactggc aaatgcagtt gtaaaagtaa gatcaccact tattttcagc
     4861 cagatcgttc cgcagatcaa tgcagcaaga gagcccatta gattggcaaa gattgcatat
     4921 aagtaatgag catccgttct ttcgatcatc caaccagtta tccacgtttg taagagaaat
     4981 ccaatcaaat atccgccagt tggcccaaat aaaacagcta tacctccagt catccctgaa
     5041 aatacaggaa gaccaatcaa gccaagtaaa agataaactc caacagatat caatcctagt
     5101 tttcttccga gaaaagttgc tgttaatcca atagcaaatg tttgccctgt caaaggaacc
     5161 aaaccaaaag gaatggtcat ctgagaaagg acagaaataa tcgcggcaaa ttccgcagca
     5221 agtatcaatt ctttcaatgt tcggttcata acgccctctt ttcgttaact taatattttt
     5281 atttggttaa cgaaattgta aaacaaagtg attactttgt aaatagaaaa tataaaaaaa
     5341 gaccagctat ccaatagata gcggtctttt tcaagaaagc ttttgaaatt ataagctttg
     5401 gttgtagtat tcaacgacaa gagcttcgtc gatttctgga tatagttcgt cacgttctgg
     5461 taaacgagtc aatgaacctt ccaatttttc agcgtcaaag cttacgaatg ctgggcgtcc
     5521 aacagttgct tctacagctt cttttactgt tacgatgttt tgtgattttt cacgaactga
     5581 gatcacttga cccacttcta cgtggtatga agggatatca acgcgtttgc catcaactaa
     5641 aatgtgacca tggttaacta attgacgtgc ttgacgacga gtcgttgcaa gtcctaaacg
     5701 gtaaacaaca ttgtctaaac gttgttctag taagatcatg aagttaacac catgtttacc
     5761 ttctttgatt ttgctagctt ttacgaacaa gttacggaat tgacgttcgt tcattccgta
     5821 catgtgacga agtttttgtt tttcagttaa ttgcatgccg tattctgatt gtttgccacg
     5881 gctattaggt ccatgttgac ctggtgcgta agggcgacgt gctaattctt ttcctgtgcc
     5941 tgacaatgag atgcctaagc gacgagaaat tttccatgat ggtcctgtat aacgagacat
     6001 taaaatttcc tccaatgaat ataagttttt ggagtaaaat aatccgttga aaaattcata
     6061 atcatatagt ttgttcttca atcttcacct ttgcagccgc ggttacacaa tcgaacccga
     6121 gacggggcaa caaactaggg atgagttatt atttttctgc tgcattattt tacacaaatt
     6181 acagtatatg atagatcatc cacgaatgtc aagataatta gtacataaaa acgctttaca
     6241 aacctaggct gtctttataa aatgagaggg tatgtgcaaa tggaaaaaag cgaaggaagt
     6301 ttaaggagaa aatcaatgaa tccggtatca ccattatttg agaaaatcga tgcttcaatt
     6361 gaaaagaagg cgaatctttt tgaaaacagc ttttcacgtt acgctgttcg agcggttctc
     6421 gcctgtttgt ttttgacttt aggaacggct gtggcatttg cgatcgcgat gaagggggaa
     6481 gatatcgcgc atggtctagg aaagatttta tacgcattta tgttcagctg gggtctggtc
     6541 atgattctat acatgaacgc cgaattagga acatcgaata tgctttatat gaccgtgggt
     6601 gtttttcggc agagacttgc tttaagtaag gcagccaaga ttctatttgc ctgtattttt
     6661 ttcaatatga ttggcggtat attatgtgga tttctaattt ctatgactac gccatttcaa
     6721 gatttaacgt tggacaactt tatgttagaa tcaatagcag taaaactagc aaaaccttca
     6781 gcaacgattt tggtggaagg gatgtttgcg aatatcgttg tcaataccgc tgtactgatc
     6841 agcatgcgga tgaaagagga tgtcggaaaa gtgatcacag tcgtattcat cattttcatt
     6901 tttgcttttt taggatatga acacgtgata gcgaattttc ctgcctttac gcttacttac
     6961 tttgcttctc acggacagat ggatggaatg acagtcagca atgtgttaca taatttgttt
     7021 tttgctttgg caggaaatta tattggtggc ggtttagtaa tgggactggg atatgcttgg
     7081 ctgaatcagt caaaatcatc ctatgtggac taaaaagtcg ttcattaaag gggaagtgcc
     7141 atggaagttt tagtttttcc aaaaaatgag gaaaaagcgt tacaaatgga aaagtacatg
     7201 aaaaatcttt tttcctttgc tggagtacct aagccggaac gcgctctttt acaacaaccg
     7261 ttactggatg agagcaaaaa tttatctatt tctgaagttt tagcttttgt tcattattat
     7321 tatgaacaaa tcgaaagaga gtatcagtat gtagcgatcg atttagtcca gaaaaacatc
     7381 aaacggctta catttgaaga cttagtcgta ttgttgccac tagttcagaa aaaagaatgg
     7441 tgggacagca tcgactcttg gagaaaagta tacagcacat ggtgtaaagc ctacccagag
     7501 cagcgtgaag aagtctttca attgtttgaa aaaaaggaag atttttggct acgtcgaata
     7561 gcaatcacgt tgcaattagg ttataaacag caaacagaca caggactatt gatccgagca
     7621 atagacgaag atcgtcaaac aaaggaattc tttatccaaa aagcaattgg ttgggcatta
     7681 cgcgattata gtaagaccga tgccgattgg gtaaaaagac agctgacaac agatttgagt
     7741 aagttagctg ttcgagaggc aagtaagtat ttataagaaa gcgagacgac aagagatgag
     7801 acgcaaatta tttgcttttg atattgatgg aacgttgtta gattcaaaca aacaagcttt
     7861 agatagcaca agagaagcat tggaaaaatt acgcaaagcc ggtcatctgg tcacgattgc
     7921 tacagggcgg agccgatttc atgcgcaaga gatcattcgc gatcttgatt ttacgaacta
     7981 cattttatgt aatggagcgg ccggtttttt agaccatgaa caagtatata agaatttact
     8041 agatcgcgac cagttggatg cgttcgttca agaagcgcat catgagcaga tcgatacagc
     8101 ttttgtcagc ttagacaata tcaagcgttc ttcttccaat gatgtaacgg tgatggaaga
     8161 agcgatgcat tctttcggtg ctgtcttgcc tgaattagat cagttttttg cagaggaaca
     8221 agaaatatac caagcgttag catttttcga tcaaacatat gatgggaagt tctcttcata
     8281 tgataaaatt cgatttgtcc gctggcatga gaacagtgta gatgtcgtac ctaaaaatgg
     8341 ttctaaagca gcgacaattc tgcacttagc agaacaagta ggcatccctc atgaagacat
     8401 catcagcttt ggcgatggac aaaacgaccg tgaaatgttg cgcatgtcag gaattgggat
     8461 agctatgggg aatgctgtgc cagaagtaca agcagaagct aagatggtca ctgatacaaa
     8521 cgatcaggat ggaatttgga aggcattgaa agagttaagc gtgatttaaa agaggttgtg
     8581 aaaaagctgt tctgcatcaa gtaataagcc cagccaaaca aaaatagctc ctcctatttt
     8641 tcgtttttct gggcttatta ccgcagatgc aagctttcga acaccgttta aggaagaggt
     8701 cgtgacagaa gtggtcagtt tcaagaaata agaagggatt tacgaaaatt gcttctcaaa
     8761 tttttgtaaa attacggctt atttctgaag aagtagatga aacaacgttt attcgggaaa
     8821 tgggactgtg acaagatgtt tgtcatagtc ccattttttt attttatttt tcaaaactaa
     8881 gaaataccag gaaagagttc cttttgtttg accttttctt tcttattgaa aacaactata
     8941 atatagagtg atgttaaaag agaagaggaa tttagtgtgc gggtattgtt tattggtgat
     9001 gtagtaggtt caatgggacg agagatgatt actgaatatc ttcctagatt gaagaagaag
     9061 taccgtccgc aagtaacgat tgtaaatggt gagaatgccg cttctggacg tggaatcaca
     9121 gaaaaaatct ataaaaaatt tttgcaagac ggcgtagatg tcgtgacgat ggggaatcat
     9181 acgtgggaca accgcggaat ctttgagttt gtaaatgaag ctaaaaagat ggttcgtccg
     9241 gcaaattttc cggaagggac gcctggacaa gggatggtct ttgtcaaagt gaaccaagtc
     9301 gaactggcag tgatcaacat gcaggctcgt tcttttatgg tggacttgga tgatcctttt
     9361 cgtaaaatga atgagctggt agaagaagcc agaaaacgta cgccgatcat ttttgtagat
     9421 tttcatggag aaacaacaag tgaaaaacaa gcaatgggct ggtttttaga tgggaaagtt
     9481 tcagcagtgg tcggtacaca tacacacgtt cagacaaatg atgcgcgaat tttgccaaga
     9541 ggaacagcgt atctgacaga cgttgggatg acaggtccct atgatggtat tttgggtatg
     9601 cgcagagaac ctgtaatcga aaagtttctg actgcattac caaaacgttt tgaagttgtg
     9661 gaacaaggcc gatcgatttt aagcggctgt atcttggaat tggatgatac gacaggtcat
     9721 gcaaaagaaa tccagctgat tcagatcaac gatgatcgac cgttcatgga gtagaaagag
     9781 aggcagtttt ttgcagcagg agccggaaat tcaaaaagaa gtaaggaaac tgacaaaact
     9841 tttaagagag aatgaaacga tcattcgcta caaagaactt gaagaaaaga tccagcaaaa
     9901 ccagtattta gctgagttga gagaaaaaat caaacaagcg caaaaagatg ctgttcattt
     9961 tgcccactat gacaaaccag cagcagaaaa agaagcaatc aaacaagcgg atcaattcat
    10021 gcaagaattt gatcagcatc cgcttgttgt agcttataga aaacagctgt tggaagcaga
    10081 tgatctgctt catcatttaa caactatgat acaagaagaa atcaatggac aaatagagga
    10141 ggaaaaacat gcctcaaaaa actaaaaata ctcctatgat ggaacaatat ctaagcatca
    10201 aggcccaata ccaagatgct tttttgttct atcgtttagg agacttttat gaactatttt
    10261 acgaagatgc gatcaaagcg tcacagcttt tggaattaac gttgacgagc agaaatcgaa
    10321 atgcagaaga acctattcct atgtgcggtg tacctcacca tgcagcacaa ggatatatcg
    10381 atgccttgat tgaaaaaggc tataaagtag cgatttgcga gcaggtagaa gatccaaaga
    10441 cgactaaagg gatggtcaag cgcgaagtga tccagctagt cactcctggt acagtcatgg
    10501 acagcaaagg attgtcggca aaagacaaca attttttaac agctgtagtt agtcaaggaa
    10561 atgaatttgg ctttgcttat gccgatttaa gtacgggaga attgaagacg gctcggttgc
    10621 atgatgaaga agctgtttta aatgaagcct ctgcattgca gacaaaagaa ctcgtattag
    10681 gcagtgagat cactgatacc cttcgtgaac agctaagcgg gcgtttaggg ctagtctttt
    10741 ctcaacaaaa tgagatggaa gaaaacgcgg aatttagctt tttgacaagc gaactagtcg
    10801 atccgctgga aaaagaagca accggcaaac tattgactta tttatcggtg acacaaaagc
    10861 ggagtttagc tcatatccaa aaagcagtag aataccaacc agatcatttt ttgaaaatgg
    10921 attattattc aaaattcaac ctagaattaa gccaatctat tcggactggg aaaaagcacg
    10981 gcactcttct atggttgtta gatgaaacaa aaacagcaat gggtggccgt ttactaaaac
    11041 aatggctaga ccgtccgctt atccaggcaa aacaaattaa agcaagacaa gaaatggtcg
    11101 cttctttgct agacagctat tttgaacgta tcgatttgca gtcagcatta acgaaagtct
    11161 atgatctgga acgcctagca ggtcgagtag ctttcggtaa tgtcaatggg cgagacttga
    11221 tccagctgaa aacttcttta gaacaagtgc caatgatcag agggttgatt gaaggaatcg
    11281 atcaaggtca atggcaaagt ctgctttcag aaatgcagcc gatggaccat ctcgtccaat
    11341 tgatcgacca agctatccaa gatgatccgc cgttacaaat aacggaagga aacatcatca
    11401 aagatggatt caatgagcag ctcgatatgt accgttctgc tatgagaaac ggaaagaaat
    11461 ggttggcaga acttgaagca aaagagcgtc aagaaacagg aatcaagaac ttaaaaatcg
    11521 gatttaaccg cgttttcggt tattatatag aaattacaaa agcaaatctt ggaaatgcag
    11581 aactggagaa gtatgaacga aaacaaactt tagcaaatgc agagcgtttc atcacaccag
    11641 aactaaaaga attggagaca caaatcctcg aagcagaaga aaaatctgtt gatttagaat
    11701 atcagttgtt tttagctgtg agagaagaag tcaaaaaggc tatccagccg ctacaagtac
    11761 ttgcaaaagc aatcagtgca gtagatgtgc tacaaagttt tgcaacgatc agcgaacgct
    11821 atcagtatgt tcggccagaa cttgtcagtg acaagcatca gcttctgatc aaagacggac
    11881 gacatcctgt agtagaaaaa gtattaggac atcaggaata tatccctaac agcgtagaaa
    11941 tggcagaaga cgaaatgatc ttgctgatta caggaccgaa tatgtctggt aaaagtacgt
    12001 atatgcgaca gttggcatta actgttctga tggcgcaaat gggctgtttc gttccggcaa
    12061 aacaagcagt tttacctatt tttgatcgga tttttacccg aattggtgca agtgacgact
    12121 taattgctgg acaaagtact tttatggtag agatgatgga agccaatcaa gccctgcgtt
    12181 atgctacgcc aaacagtttg attttgtttg acgaactagg gcgaggaaca gcaacctatg
    12241 acggaatggc gttggcccaa gcaatcatcg aatatatcca tcgtcatgta caagcaaaaa
    12301 cactcttttc tactcattat catgaactga cggtacttga gaaagaactt ccgcagctta
    12361 aaaatgtcca tgttggagcc gttgaaaaag acggagaagt tgtcttcttg cataaaatga
    12421 tggatggacc tgcggataaa agctacggca tacatgtggc aaagatcgca ggattgcctt
    12481 ccaatctact agaacgagca gcagatatcc tgcatacgct agaaaatggc gataacggac
    12541 accatactgt tccagctgaa gtagaagaga aaactaaaac agccaaacag gagcaaataa
    12601 aaccacctgc agaaaaagta aatcaagacc atatacaaga ccgtgtaaaa gaagaaaacc
    12661 aacagctttc tttattcagt gaattatccg aaaatgaaac agaagtcatt gaatcactga
    12721 agcaaataaa tttactagaa atgacgccaa tggatgcact aaacacatta tatgaattac
    12781 aaaaacaact gtaggaagaa ggcggatatc gatggccaaa atccaagaat tatctgaacg
    12841 tttagctaat cagatcgctg cgggagaagt agtggagcga ccagcttccg ttgtgaaaga
    12901 attagtggaa aatgcaattg acgccggcag tactcagatt gatatcttag ttgaagaagc
    12961 gggactgcgg acgatccaag tgattgataa cggagaaggc attttggcag acgacgtaga
    13021 gaatgccttc aagcggcatg cgacaagtaa gatccataat cgcgacgatc tttttcgcat
    13081 acgaacgctg ggctttcgag gggaagcttt gccaagtatt gcctctgttt ctgaaatgac
    13141 tctggaaacg gcgaatcagg aagaaaaaca aggcacctat ctgtcgctga aaggcggaga
    13201 agttattgag catcgaccag caccactgcg tcaaggaacg aagattaccg tcagcaatct
    13261 gtttttcaat acgccagccc gcttgaaata cgtgaaaaca ctgcacacag aattagcgaa
    13321 catcggggac atcgtcaatc gactggcatt gagtcatcca tcgattgctt ttcgtttagt
    13381 acatgatgga aacaaaatgc tggcaactgc tggaaatggc gatctgaaac aaacaattgc
    13441 gggaatctac ggattatcta ccgcaaaaaa aatgctgaaa atcgaaggaa aagatctaga
    13501 ttttgagtta tttggctatg tttcattgcc tgaggtgact cgtgccagta ggaattactt
    13561 atcgacgatc atcaatggac gttttatcaa gaacttttcc ttgaataaag caatcgttgc
    13621 aggttatggg tcaaaattaa tggtcggtcg tttccctatt gctgtattgg aaatcaaaat
    13681 ggacccgctt ttagtagatg taaatgttca tccgacgaag caggaagtcc ggctgtcgaa
    13741 ggaaaaagaa ctaacagagc taattagtaa cgccattcaa aaagcattgt ctcaagaaaa
    13801 tctgattcca agtgcagcgg ataatctacg gtttaagaaa aaagtacctg atcagcctaa
    13861 aacagaacaa atcgaaatcg atttgtccta cgcttttgaa gaagaaaaac ctaaaaagcc
    13921 aagtagtttg aattttgatc gagagacagg tacctttttc gtagaagaac taaagaacac
    13981 atttgttgac agaattgttg ataagtctgt ggaaaatgtt gaaaactatt ctggtttttc
    14041 tgaagaaaac accacttatc cagaaaaaac agcgaaaacg gtggataagt ttggtgaaaa
    14101 tgttgataac tttgtagaaa aaacagtgga aaaatctgaa aagaaagaat ttcccattgt
    14161 ggataacatg gagaaaacaa atgaagaaca agcagataaa tccgtagaag aggatgaaaa
    14221 gatttacgag gaagaattga cactccatcc tgaatttgat ttcactcaag gaaacgacaa
    14281 aacattagaa caagccatca agaaattaga aacggaaaaa ccagtacaac gttttcctca
    14341 gttggaatat tttggacaga tgcacggaac gtatcttttt gcccaaagta gcgatgggtt
    14401 gtacatcatt gatcagcatg ctgcccaaga gcgcattaag tatgaatatt tccgggaaaa
    14461 aattggggaa gtatctgatg atctgcaaga attgcttgtt ccatttgtgt tagactatcc
    14521 aaacagtgat gctttgaaat taaaagagca aaaacacttg ctagaagatg cagggattta
    14581 tttagaagaa tttggtcaaa acagctttat tgtacgggcc catcctactt ggtatcccaa
    14641 tggagaagaa gaatcgatta ttcgggagat gatcgatatg ctgctgacaa ctggaagtgt
    14701 gagtgtaaaa aaattccgtg aagcaacagc aatcatgatg agttgcaaac gatcgattaa
    14761 agccaaccat tatttgaatg aagcacaagc acgtgtgctg ctgaaggatc tggcacgatg
    14821 cgaaaatcct ttcaattgtc cccatggccg tccagtattg attcacttta ccaattcgga
    14881 tatggaaagg atgttcaaac gaatccaaga cccgcattaa gtcaaagaga agagggaaaa
    14941 catgaagatt gtattagcat cccaatcacc aagaagaaaa gagttattgg ctcatatcgt
    15001 ttcagaattc gagattgaac ctgctgatat cgatgaaacg ccatttccag acgaacagcc
    15061 cgtcgattat gttcgtagaa tggctgaaga aaaagcacga actgtctggg aacaagatga
    15121 aaaagaagac catttagtga ttgccagtga cacgactgtt gttctcaatc aagagatcat
    15181 gggtaaacca gaagatctgg ctgaggctga attcatgctg aaaaaactaa gcggggaaac
    15241 acatactgta tatacagctg ttgttttaaa aacaaaggaa cgagaagaac ggatactagc
    15301 agaagcacat gtcacttttt atcctttgac agacgaagag atcaaacgct acttggagac
    15361 gggggattat gcggataagg caggggcata cggcatccaa aatgctgcga gtgtattcgt
    15421 caaagaaatt tctggagatt attacagtat cgtaggattt ccgattggtg ctgtcaatca
    15481 ggcactgaaa acattcattt aaacaaaaaa gcggactatc tgattttcga ttgaaaatcc
    15541 atgatagccc gtttttttta ttgaaaagat tacttatcta ctctgactaa gattttactt
    15601 cagctttacc gactgcttcg cccgggcgaa caagtcctga ttggtcaaga gaaagtcctg
    15661 caactttttc attattggtc aaagcaacaa taatgtcagt tttcttgcca gcttgttcta
    15721 tttctggcaa atccatttca gcaatcaggg tctctggtgt gacaaggtat ccttctttca
    15781 cgaatacatt gaaaggaaca ccctttagtt caacagtatc taatcccatg tgaacaagta
    15841 cttccagccc ctcattagaa agaataccaa tggcatgttt tgtttcaaag acactcgtca
    15901 ctttaccgtt tactggtgca tagactttgc cgtttgacgg ttcgacagca taaccgtcac
    15961 ccatcatttt ttgtgagaaa actgggtcat ttacttcagt tatttccttg atggtccctg
    16021 cagcgactgc gaataatgtt tcttttgttg caactgccgt accagttgct gaaacaggtt
    16081 cgaaagactg tggcgttgct gttgcgccat ttgctgtttg aagttgtaca tcagcagcat
    16141 tttcaggaac agcaggatca atcttattca aaattccctg acgtctaaag atgatcgtca
    16201 agccaaatgg aatgatgaca gcaatgatca ttgcgatgat gaaaggtacc catgtttctg
    16261 cttggatagc tagtattcct ggcaaaccgc ctactccgat cgcgttagct cgaacaccca
    16321 tcaagttagc aaacatgcca gctaatccag agccgaccat tgcagcgacg aaaggataga
    16381 cgtatttcaa attgattccg aacatagcag gttcagttac gcctaagtaa caagaaatca
    16441 ttgctggaat cgagatttgt tcttcttttt tattcccacg atgtaagaag atgatggcta
    16501 agactgcaga gccttgggcg atatttgata aagcaatcat cggccataaa ttcgttgatc
    16561 cgaaatctgc aatcagctgc atatcgatgg cattggtcat atggtggaga ccagtaatca
    16621 ctaaaggagc atacaggaat ccgaatacgg cactaaacaa ccagctgata gaagacgtca
    16681 atccagtatt tacgatattt gagatccagc ttccgactgt ccagccgata ggacctaaaa
    16741 taacgtgtgc agctaaaaca gttggtaaca atgaaaacaa tggaacaaaa atcatcgaga
    16801 tcgactgagg gatatatttg cggaagaaga tttctagata cgctaacata aaccctgcca
    16861 gcatggctgg aataacttgc gcttgataac caatcatctg gacttgagca aaaccaaaat
    16921 cccaaaatgg aatatctgct gcggctgtgg aagctacact ataagcattt aataattgcg
    16981 gagaaactaa cgtaattcct aaaacgattc ctaatatttg tgtagtaccc atcttttttg
    17041 caatactcca agtgatacct actggaagaa aatggaaaat tgcttcaccc ggtaaccaca
    17101 aaaaggcatt gacaccattc caaaacttcg agtgttcaac gattgttcca ccaagacttt
    17161 caaactggat accttcaagt acatttctga atccaaggat caatccaccg acaatgattg
    17221 caggaatcag tggtgtaaag atttctgcta aaacggcaat agcacgttga acgggatgaa
    17281 ggttttgttt tgcagctgct tttccttgtt ctttggaaac cccttcaact ccagatactg
    17341 caacgaagtc gttataaaaa gtagaaacat cgtttcctat gatgacttgg aactgtcctg
    17401 cattcgtgaa catccctttt acgctaggta tatcttcgat cgctttttcg tccgcttttc
    17461 ctggatcatt caggacaaaa cgcatacgag tcgcacaatg actaactgca gcaatatttt
    17521 cttttccgcc gatatcatgt aacagcttct cggcatctgc ttgatatttt gccatatcaa
    17581 taaacccctt tctcttaact tgtctatgca agttgtttct tcatttacta tacttttttt
    17641 cggagagaat tgcaagcgtt ttattaaaaa gtacgagaga aaatgatatc gttttaaaaa
    17701 gcttaaaata agcttttctt aaaaatagga acattgaaat acaaaaagag ctgtagtata
    17761 gtgaagaaat gatggaagaa cgagtgaggg aaaaaacatg aataagttca atgaaatttt
    17821 tttagatttg gaacagaaaa ttttagcgaa agaatatcct ccgcattcgc ttttacccag
    17881 tgagaaccaa ttgatcaaaa tttatggtgt ttcacgagaa acaattagaa aagcgctgaa
    17941 tcttttgact acggaaggtt atattcaaaa aaaacaagga aaaggttcga tcgtactgga
    18001 tcggaatcgc ttcgactttc ctatttctgg attgactagc tataaagagc tacaggagac
    18061 ccaacgtatt ccaagtgaaa cgatcatcca tacgttagaa gaaacagaag tgacaaaagc
    18121 attgaacgag atcactggct gggagatcgg tgcgcctgtt tggcatttga tacgcgaaag
    18181 aaaaatcaat ggcgaagtag ttatattaga caccgattat ctattaaagg aaatcgttcc
    18241 tattttaact ccagcacaag cacaaggatc gatctatgaa tattttgaaa atgagctttc
    18301 tttgacgatt gcttacgcgc aaaaggagat cacagtagaa gaagtaacgg aaatcatgaa
    18361 aacagcgatg gatcttcacg atgacactta tgcagtagta gtcagaagcc tcgttcatct
    18421 ggaagatacc cgctgtttcg agtattctga gtcggttcac cgattagata aattccgttt
    18481 cgttgagttt gcccgacgcc gaaaagtgta aatagttcaa aagaaagcaa attgttggca
    18541 tctgtccact ttggcgctat gataggatca caaagggggc atttttaatg gataaaccaa
    18601 taagaccaga aaaagaagaa ctgaaagaaa agttatcacc catggcttat gcagtcacac
    18661 aagaaaatgc aacagaacga ccatttagcg ggaaatatga tgatttttac gaaaagggga
    18721 tctatgtaga tatcgtcagc ggtgaaccgt tattcagctc tgcagaaaaa tatgatgcag
    18781 gttgtggctg gccatcattc agcaaaccaa taacgaaaag acaagtgcgc gaaaaagccg
    18841 atttttctca tggcatgcat cgaatagaag ttcgaagcaa agaagcagat tctcatctcg
    18901 gacatgtgtt taccgatgga ccagtagatc aaggtggtct acgttactgc atcaattctg
    18961 cctcactgaa attcattcca tatgaaaaga tggatgaatt aggttatggg gaattcaaat
    19021 cacttgtaga ataatggaaa tacacaaaga gcggtacaat acggggattt actcgatcgt
    19081 ccgctctttt tttattcaag cgattgctat ttgaaagaaa cgctccctag actccttttt
    19141 tcacaaggtg aaatgtgcta caataaaggg aacgtatgtg tgcaaaagaa agaggactag
    19201 aatatgtatg aatatctgac aggtgtggtt acctttatca acccctatta catagtagta
    19261 gaaaccaatg gtatcggcta ccagatcgca ctaggaaatc cttaccgcta cagtagcaaa
    19321 ctaaatcaaa aaataaaaat ttatgtccat caggtaatac gtgaagatgc gcatcttctg
    19381 tatggatttg attcgctaga agaaaagcaa ctgtttttgc gattagtaag tgtttcagga
    19441 atcggtccga aaagtgccct tgcaatcatg gcaagcgaag accactccgg tttgattcaa
    19501 gcagtagaat ccagtgatgt cacttatttg acgaaatttc caggagtagg gaaaaagaca
    19561 gcacaacaaa tgatcttgga tctaaaagga aaattcggtg agctgagtat cgatacacct
    19621 tttagtctat ttgatgaagc aaacacaaaa gacgctacag ctctatctga agcaatagaa
    19681 gctctctcgg ctcttggtta cagtgatcga gaaatcaaac gtgtagaaaa acaattaaaa
    19741 gaagtagata atcagacaac ggatgagtat ctacgccaag cactcaagct aatgatgaaa
    19801 aaataaggag gagaccaaat gacagatgaa tacttgttat caccagaatc tggcgaaaat
    19861 gaactgagtt tggaaaaatc tcttcgacca cagctgcttt ctcaatatat cggacagaga
    19921 aaagtcaaac aagaacttca gatttacatc gaagctgcca gaaatcggga agaaccttta
    19981 gatcatgttc tgttgtacgg gcctcctgga ttaggaaaaa cgacgatggc catggtcatt
    20041 gctaatgaga tgaccgtaaa tattcgtacg acgagtggcc ctgctatcga acgtccgggt
    20101 gacttagtag caatcttgaa tgaactggaa gcaggggatg tcctgtttat cgatgagatc
    20161 catcgattac ctagagtggt agaagagatg ctttattcag ctatggaaga tttctatgta
    20221 gatatcatgg taggacaagg gccaaccgca catccggttc atttccctct tcctcccttt
    20281 actttgattg gcgcgacgac acgggcaggt atgctttctg cacctttacg agatcgattt
    20341 ggcatcattt ctcatatgga atattatgac gaacaggatc tgcaggagat cgttttacgt
    20401 tcttcggata tttttcaaac agagatcatt gaagaagggg cattggaaat tgctcgccga
    20461 tcacgaggaa cgcctagaat cgctaatcgc ttgctcaaac gggttcgtga ttttgcgcaa
    20521 gtccaaggag acgggaaaat cgatcgtcct atagcggatc aggcacttac tttgctacag
    20581 gtcgatcagg ctggattgga ttatgttgac cagaaattgt tgcgaacaat gatcgagtta
    20641 tatggcggcg gaccagtagg gttaagtacg ctttcagtaa acataggaga agagcgtgag
    20701 acggtagaag atatgtatga accgtatctg atccaaaaag gatttttaaa gcgtacacct
    20761 agagggcgaa ttgctactgc ttatgcctat gaacattttg gttacgacta tcatatatga
    20821 tttttgcttt tagattggag gaaaatgaat gagtgataca acacgtacga aacaattgat
    20881 caaaaacgta ttgattgatt tgtgccaaac gcaatctttt cgaaaaatca gcgtccaaaa
    20941 aatcgcaaac gaagcaaaaa tcaatcgaca aacattttat tatcatttta cagataaata
    21001 cgatttgctt cgttgggtct attgtgagga tgcattgcat tatttagatc cacaatgtct
    21061 ttcacttgat aattgggaag agcaagcatt gaaaatgctg aaagcaattg ctgagaagaa
    21121 agaattttat gcaaatactg tgctttctga cccagaaatt ttacaaaaag agttttctca
    21181 actgatccaa aaatcattta tcaagatttt tgaacaaatg gatgaagaaa gacaactttc
    21241 tgcaagagac aaactatttt atgctcgttt tttttcttac ggttgcagtg gtgtattagt
    21301 caattggatc acgaatgaat atgaagaaac accgatggaa atcgctatgc agcttttccg
    21361 tttagccaag gatacggaat tatattctta tcgtctttat gcaaaagaag agttggaaga
    21421 acagtaatag aaaaattttt tgttggaaaa gcaataatcg ctaaaaggag atggggaaat
    21481 gacaaaaatt ttatttgttt gtttgggaaa tatttgccgt tcaccgatgg cagaaggctt
    21541 gctgagaaaa cgaatagcat tagaaggaag ggaagcagaa ttttttgtgg attctgctgc
    21601 tacaagcacg tatgaagttg gaaagacacc gcaccctgga acgaaaaaaa tattaaacca
    21661 agaaaaagta gatatgacga atatggttgc tcggcaaatc acaccccacg attttgaaac
    21721 ttttgattgg atcattggca tggatcaaga aaatgtagaa gaactgaaac gaagagcacc
    21781 acgctcagcg caggggaaaa tccatttgtt tttaagtagc attcctggaa aagaaaaaga
    21841 aaatgttccc gacccatact ataccaataa ctttgatgag acatatcact tgatcacaga
    21901 aggcctgaat cattggtgga atatttggat tgatgaaaat aaaataacaa ataatttgta
    21961 aagttaacgc tttcttttta aggcgatgtt agagcatttc cttgtttttt tgtaaaatgt
    22021 taggtaaaat aaaattaatt aagtaacact ttcttagcta aaggaggagt gcattcgatg
    22081 aacgaagaga cagtatcgcc aatgaagaag cttacgaagt tagttactca tacttcgtat
    22141 caactttttg attcttttgt aaatgacact gaagaacatg ctaagaatgt acgattgcaa
    22201 ggaaagttta ttcttgagct gcaaaaagct gctactgaac atagtctagt tatcttacaa
    22261 gtaagagcaa atcgtttgag tagcaagtat gaaacgatct caggctgggt ggcgacaaaa
    22321 acggtcgaaa aagaccgagt gatgatccgc ctacaaaacg aagagcagca gatccggatg
    22381 gttcctgtag aacaaattga aaaaataaca atcctttcac cagcagggga acaggaacgg
    22441 ttatcaaaat aatttatacc agatagaaaa gcacgtatga gcttgtacca tcaatcatta
    22501 ctaatcaaaa ataaccgaat gctttaagct gatatcagta aaacggaacg ttttaggatg
    22561 agtatcatag catgacagca ttcggttgat tagttgaatc gattggatgg tacaagtttt
    22621 tttgataaaa aaaggtcctg ctagccgggg agctagcagg ataaggagtt aaaaatgaaa
    22681 aagtgttgtt agggttgttt gttggtatgc ttatataata gtcattagtt gtgaatattt
    22741 tgtgacgaaa gttttcgaaa gttgttaagt ttt
//
LOCUS       DADJYG010000030        22185 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.030, whole genome shotgun sequence.
ACCESSION   DADJYG010000030 DADJYG010000000
VERSION     DADJYG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.030"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            55..1953
                     /locus_tag="JZ382_000955"
     CDS             55..1953
                     /locus_tag="JZ382_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAY2427158.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2252..2971)
                     /locus_tag="JZ382_000956"
     CDS             complement(2252..2971)
                     /locus_tag="JZ382_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAY2427159.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3308..3640
                     /locus_tag="JZ382_000957"
     CDS             3308..3640
                     /locus_tag="JZ382_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427160.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3627..5630
                     /locus_tag="JZ382_000958"
     CDS             3627..5630
                     /locus_tag="JZ382_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAY2427161.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5649..6119
                     /locus_tag="JZ382_000959"
     CDS             5649..6119
                     /locus_tag="JZ382_000959"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAY2427162.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6140..6724
                     /locus_tag="JZ382_000960"
     CDS             6140..6724
                     /locus_tag="JZ382_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAY2427163.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6739..7725
                     /locus_tag="JZ382_000961"
     CDS             6739..7725
                     /locus_tag="JZ382_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAY2427164.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7715..8026
                     /locus_tag="JZ382_000962"
     CDS             7715..8026
                     /locus_tag="JZ382_000962"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAY2427165.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8048..9829
                     /locus_tag="JZ382_000963"
     CDS             8048..9829
                     /locus_tag="JZ382_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAY2427166.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9822..11198
                     /locus_tag="JZ382_000964"
     CDS             9822..11198
                     /locus_tag="JZ382_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAY2427167.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11205..11840
                     /locus_tag="JZ382_000965"
     CDS             11205..11840
                     /locus_tag="JZ382_000965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAY2427168.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11870..13225
                     /locus_tag="JZ382_000966"
     CDS             11870..13225
                     /locus_tag="JZ382_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAY2427169.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13417..14811)
                     /locus_tag="JZ382_000967"
     CDS             complement(13417..14811)
                     /locus_tag="JZ382_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAY2427170.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14903..15874
                     /locus_tag="JZ382_000968"
     CDS             14903..15874
                     /locus_tag="JZ382_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAY2427171.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15871..16653
                     /locus_tag="JZ382_000969"
     CDS             15871..16653
                     /locus_tag="JZ382_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAY2427172.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16822..17193)
                     /locus_tag="JZ382_000970"
     CDS             complement(16822..17193)
                     /locus_tag="JZ382_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY2427173.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17327..17770
                     /locus_tag="JZ382_000971"
     CDS             17327..17770
                     /locus_tag="JZ382_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAY2427174.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="JZ382_000972"
     CDS             complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="JZ382_000972"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY2427175.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18641..20008)
                     /locus_tag="JZ382_000973"
     CDS             complement(18641..20008)
                     /locus_tag="JZ382_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2427176.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19992..20822)
                     /locus_tag="JZ382_000974"
     CDS             complement(19992..20822)
                     /locus_tag="JZ382_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAY2427177.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20985..21422
                     /locus_tag="JZ382_000975"
     CDS             20985..21422
                     /locus_tag="JZ382_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAY2427178.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21498..22118
                     /locus_tag="JZ382_000976"
     CDS             21498..22118
                     /locus_tag="JZ382_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAY2427179.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7116 a         3850 c         4783 g         6436 t
ORIGIN      
        1 atagagcata acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac
       61 cgctatttag atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc
      121 atcaatctcg aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata
      181 catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa
      241 aagatcaaag accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt
      301 tttacggctt atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc
      361 cagctgatcc atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca
      421 aaagttcgaa aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc
      481 gatgatcgta tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg
      541 atgaaggaag aagaaaaatt tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc
      601 gatcatttgc atatcgtggg tgatattttt gatcgaggaa ctcaagcagc caaagtaatg
      661 gatcgaatca tggatttttc ctctgtagat atccagtggg ggaatcatga tattttgtgg
      721 attggtgcgt attgcggctc tgaggcatgt ttattgacgc ttttacggat tgccgcacgc
      781 tacaactatt tatttgaatt agagaaagaa tatggattaa atttacgtcc attattttct
      841 tttgcccatc aaacctatca gaagaatcca gtatttaccc ctaaaaacag gaaagatcta
      901 tctgaaagag agcaagaaga attagaaaag atccatcaag cactttctat cctccaattc
      961 aaatcagaac atcagttgtt gaatcgacga cctgaattta aaatggacga tcgtaaattg
     1021 ttggaaaaaa tcaactatga ggaatcaacg atcgttttag aaggtcagct ctatgagttg
     1081 aaagggactt gttttcaaac gatccagcca gatgatccta aaaaattctt ggaagaggaa
     1141 caaaaagtga tcgatagttt gatgtattca ttccagcact cgttaagaat gcaaaaacac
     1201 atgacgtttt tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtactgttc
     1261 catggctgta tacctgtaga tgaaaagggg cagctcttag catttgaatt agaccaatct
     1321 tatcgcggaa aagaactgct gggatttttt gaaaaacaga taactgaaag cgcaaaagat
     1381 ttgactgcaa atgaagattt ggcgaccgat ttgatttggt atgcgtggag cgggccattt
     1441 tcgcctttat tcggcaagag taaaatggca acttttgagc gctattttct cacagagcct
     1501 catacgcacg tcgaaaaaag caatccctat tatcacttgc gagatgaaga gtggttcagt
     1561 gaaaaaattt tggcagaatt cgatgcaaaa gaagaaggtt cagcaatcat caatggacac
     1621 acgccagtaa aagtaaaaaa aggagagtca ccgatcaaag caaacggaaa agcatttgtt
     1681 attgacggag gtctatccaa agcttatcaa aaaacaaccg ggattgctgg ctactcatta
     1741 ctatgcaatt cttacggttt ccaaatcgtg acacattatc cgttcttgcc tattgaacaa
     1801 caatttgcaa aaaaatctga tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca
     1861 cgcaaactta atcggaatac gacaaaagga atggaattac agcaacagat tactcaactc
     1921 caaaggctgt tggattatcg aaaggacgat tgagaaaaat gctagccaac attgctcaat
     1981 agactaaaaa gcgactgcaa caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa
     2041 aacggtaaaa tattagaagc tatttttctg tttatttctc taatactcag gtcagggcgc
     2101 tgatttcacg acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
     2161 aatatccaac aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttgaa
     2221 cggcaggctc acgacctaat cagaaaaacg attaaaattc attcacaaag aaatcgaata
     2281 aacgctgttc agatgaatct acacgatgag aaagttccgg atgccattgg acaccaagga
     2341 ttttgtgcat attatcttta ctttggactg cttcaatgag tccatcgttg gaaaaggcga
     2401 tggcttttaa caaaggagaa agatctttga ctgcctgatg atgataggaa ttcacaaagt
     2461 atgaatcagg gagaaggtca cttaataaac tgtccgaaac gattttgact tcgtgagttg
     2521 caaactgcgg ggcagtgggc tgttgttcgt gttttacgga ccattttgga tataatgaaa
     2581 gatcttgata aagtgttccc tccaacacga cattcaatag ttgaagtcct cggcagactg
     2641 caaaaatcgg ttttctttgt ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc
     2701 gttgaatatt tgtttccgtc agttttggat gagggtcttc catgtaaagt tgcggactaa
     2761 catcctgacc gcctgcaaga atgagtttgt cgattttctc aattagttga ggcgctgaat
     2821 ctggagacat gactggcaga ataagaggta atccttgagc atctaaaact gcatccacaa
     2881 atccctgcgg tgtatagctt acttgatttc cttggaaagt atctgtcgct cgaatcagtt
     2941 gattcccggc aatgccgatg attggtttca tttttattcc ccctaaagta gactaattgt
     3001 aacaaggatt taaaaaaatt taaactatgt aagacttgcc cctatactta tctgttcttg
     3061 ctgtgttact atctgtaaag agaattttcg tgtgaaattc atgacgatag ttgaaaatat
     3121 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
     3181 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
     3241 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
     3301 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
     3361 tgagaagcgt gctcgaaccc tacggcagca gttgaagacc tatgcagaag aaaaagagca
     3421 agaattggag ataatccaaa cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa
     3481 agaacaattc gaaaagcaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
     3541 caaggtgcaa gcaactttga aagaccaata tcaaaagcat cagagtcaag caatcggtgc
     3601 aatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
     3661 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaatgttg
     3721 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtagaaaaa tattttccag
     3781 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca
     3841 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
     3901 tgaaacgtca agaactttcc ttacaagact tggaagcaaa ttattccgaa gaaaatttca
     3961 tgaataaatt agaagaagtc cttgatttaa aagaaaagtg ggaacagcta actgaaaaac
     4021 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
     4081 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
     4141 ttgaaagttt caaggaagaa ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt
     4201 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag
     4261 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
     4321 aagaaaaatt gcaagaaacc aaacagcaat tatccattgt tcacgagcag caaaaaaaga
     4381 taacgacagc gttaggtcag tgcagcggct atatccaaga atttgaatgg gtagaagaag
     4441 tcacacttgc cgttgcagaa cgggaaaaag taaaagaacg gtttatccat gccacttatc
     4501 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgatg catgtgttaa
     4561 acgaaactgt atcagaagaa gatatatacg ttagtttcga aacaccaaac tcagaagaga
     4621 ttcagcagga agtccctact aaattgaaaa accatccgct tatcgaacct tttgaattgc
     4681 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacgcct tggatgacac
     4741 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttacgga ttgctgatgc
     4801 tcattgggac aatactagct caaaaactat ttgttttacc ccgcgggatg aaacgtttcg
     4861 taaaattctt tgagattctc tcggtaccat cgatcatctg gggattgatc tacagttcat
     4921 ttttcggaca ggcattacca aaagaactat ttggtatccg cttgcctttc ccaatcctat
     4981 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
     5041 tcttggttgg attattcgtg gcagcaaagg aaaatatcag gaggaaagat tacttgagtg
     5101 ctgtcagtga tggttttgcc tggcaaggga tcctgattgg gatcgttatt gcattgattg
     5161 gtgtcatgtt gataaaaaat acagcatttg tttatcttgg cgcggggcta tcgatcattg
     5221 ctgctttatg tatcttaatc gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg
     5281 ccaaaggagc gtataatctt tacggattga ctggttatat cggtgattta gtcagttata
     5341 cacgtttgat ggcactaggt atttcaggcg gaagtattgg cgcggcattc aatatgctag
     5401 ttgctttcat gccgccagcc gctcgattca gtgtagggat ccttttgatc atcgctttac
     5461 acgcactgaa tatgtttttg acactgttaa gtgcctatgt ccatggtgca cgattgcagt
     5521 atgtcgaatt ttttggaaaa ttttatacag gaggcggacg tgcctttgac ccgctgaaaa
     5581 cggcggaaaa atacgtcaac atcaatcata agaaaaaaaa caataaataa ttggaggatt
     5641 tttatacaat gatggattac ttaattagtc aaaatggtgg aatggtattt gcagtattag
     5701 cgatggcaac agcgacgatt ttttcaggga ttggttcagc taaaggtgtt ggaatgacag
     5761 gtgaggcagc agcagcgtta acaacaagtc aacccgaaaa attcggacag gctttgattc
     5821 tgcaattgct tccaggtacg caagggttgt atggattcgt tatcgccttt ttgattttta
     5881 tcaacttagg aaatgatatg tcagttgtac aaggattgaa ctttctaggc gcttctctgc
     5941 cgattgcttt tacaggtttg ttttcaggta tcgcccaagg gaaagttgca gcagcaggta
     6001 tccaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
     6061 ttgaaacata tgcgatttta ggtttcgtta tttccttcct attagtattg aatgcctaag
     6121 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc
     6181 agcagagcaa gaaagagcgg cgttagagca agaagagcga atgaagatcg atcaaaactt
     6241 tgaacaaaaa cgtacgcaaa tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
     6301 attacttgaa aaaaaatacc gtcagcttag gaatcgccaa caagtagaag tacgacaaga
     6361 aaatttgaat gcgaagcagg aatttttacg acgattgttt gcagatgcaa tagctgaaat
     6421 ggagaattgg gatgaatctg aacaaatcca gtttatcaaa aattcattgt attcattacc
     6481 tttgacaggc aaagtaacat tcattgctgg agaaaaatca gcagcttatc tttctcagac
     6541 attgctggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
     6601 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
     6661 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
     6721 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
     6781 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
     6841 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
     6901 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
     6961 gcaccggagc ctgaaatcgt ttggatctat acgatgcgct atactttcca taatctgaag
     7021 gttttgacaa aagccgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
     7081 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaactccct
     7141 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
     7201 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
     7261 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
     7321 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
     7381 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
     7441 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
     7501 gagggaaatc ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
     7561 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
     7621 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
     7681 ccagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
     7741 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
     7801 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
     7861 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
     7921 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
     7981 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
     8041 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
     8101 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
     8161 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
     8221 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
     8281 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
     8341 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
     8401 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
     8461 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
     8521 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
     8581 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
     8641 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
     8701 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
     8761 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
     8821 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
     8881 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
     8941 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
     9001 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
     9061 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
     9121 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
     9181 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
     9241 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
     9301 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
     9361 aacagaagta gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
     9421 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
     9481 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
     9541 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
     9601 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
     9661 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
     9721 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
     9781 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
     9841 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
     9901 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
     9961 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    10021 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    10081 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    10141 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    10201 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    10261 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    10321 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    10381 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    10441 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    10501 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    10561 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    10621 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    10681 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    10741 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    10801 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    10861 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    10921 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    10981 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    11041 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    11101 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    11161 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    11221 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    11281 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    11341 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    11401 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    11461 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    11521 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    11581 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    11641 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    11701 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    11761 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    11821 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    11881 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    11941 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    12001 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    12061 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    12121 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    12181 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    12241 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgttatcc aattgattgg
    12301 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    12361 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    12421 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    12481 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    12541 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    12601 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    12661 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    12721 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    12781 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    12841 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    12901 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    12961 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    13021 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    13081 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    13141 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    13201 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    13261 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    13321 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    13381 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    13441 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    13501 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    13561 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    13621 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    13681 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    13741 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    13801 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    13861 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    13921 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    13981 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    14041 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    14101 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    14161 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    14221 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    14281 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    14341 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    14401 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    14461 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    14521 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    14581 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    14641 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    14701 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    14761 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    14821 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    14881 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    14941 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    15001 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    15061 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    15121 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    15181 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    15241 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    15301 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    15361 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    15421 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    15481 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    15541 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    15601 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    15661 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    15721 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    15781 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    15841 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    15901 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    15961 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    16021 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    16081 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    16141 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    16201 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    16261 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    16321 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    16381 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    16441 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    16501 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    16561 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    16621 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    16681 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    16741 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    16801 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    16861 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    16921 accggcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    16981 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    17041 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    17101 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    17161 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    17221 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    17281 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    17341 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    17401 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    17461 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    17521 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    17581 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    17641 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    17701 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    17761 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    17821 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    17881 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    17941 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    18001 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    18061 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    18121 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    18181 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    18241 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    18301 ggagatgact gccgacacgg cgtgccatca cttcgatctc taggaaatct tcatgtgtca
    18361 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    18421 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    18481 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    18541 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    18601 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    18661 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    18721 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    18781 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    18841 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    18901 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    18961 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    19021 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    19081 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    19141 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    19201 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    19261 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    19321 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    19381 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    19441 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    19501 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    19561 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    19621 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    19681 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    19741 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    19801 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    19861 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    19921 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    19981 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    20041 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    20101 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    20161 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    20221 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    20281 ttcatgactt gcccgcgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    20341 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    20401 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    20461 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    20521 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    20581 aatccacctg aatttctgat cactggagcg taacctgcat ctttcagtgt ttggatcccg
    20641 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    20701 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    20761 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    20821 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    20881 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    20941 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    21001 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    21061 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    21121 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    21181 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    21241 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    21301 aagaactgca tgttagtttg aaagaccgtc ccacaggagg gaaggttcga atcgattatg
    21361 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    21421 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    21481 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    21541 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    21601 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    21661 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    21721 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    21781 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    21841 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    21901 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    21961 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    22021 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    22081 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    22141 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacg
//
LOCUS       DADJYG010000031        21967 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.031, whole genome shotgun sequence.
ACCESSION   DADJYG010000031 DADJYG010000000
VERSION     DADJYG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.031"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            168..1040
                     /locus_tag="JZ382_000977"
     CDS             168..1040
                     /locus_tag="JZ382_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2427180.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1037..2995
                     /locus_tag="JZ382_000978"
     CDS             1037..2995
                     /locus_tag="JZ382_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2427181.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3327..4178)
                     /locus_tag="JZ382_000979"
     CDS             complement(3327..4178)
                     /locus_tag="JZ382_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2427182.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4301..4768
                     /locus_tag="JZ382_000980"
     CDS             4301..4768
                     /locus_tag="JZ382_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAY2427183.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5031..5513)
                     /locus_tag="JZ382_000981"
     CDS             complement(5031..5513)
                     /locus_tag="JZ382_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY2427184.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5613..6476)
                     /locus_tag="JZ382_000982"
     CDS             complement(5613..6476)
                     /locus_tag="JZ382_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY2427185.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            complement(7078..8157)
                     /locus_tag="JZ382_000983"
     CDS             complement(7078..8157)
                     /locus_tag="JZ382_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2427186.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            8265..9668
                     /locus_tag="JZ382_000984"
     CDS             8265..9668
                     /locus_tag="JZ382_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY2427187.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            10017..10649
                     /locus_tag="JZ382_000985"
     CDS             10017..10649
                     /locus_tag="JZ382_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAY2427188.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            11237..12676
                     /locus_tag="JZ382_000986"
     CDS             11237..12676
                     /locus_tag="JZ382_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2427189.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            12971..13189
                     /locus_tag="JZ382_000987"
                     /pseudo
     CDS             12971..13189
                     /locus_tag="JZ382_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13438..13653
                     /locus_tag="JZ382_000988"
     CDS             13438..13653
                     /locus_tag="JZ382_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427190.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(13870..14126)
                     /locus_tag="JZ382_000989"
                     /pseudo
     CDS             complement(13870..14126)
                     /locus_tag="JZ382_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(14313..15224)
                     /locus_tag="JZ382_000990"
     CDS             complement(14313..15224)
                     /locus_tag="JZ382_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2427191.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(15243..16247)
                     /locus_tag="JZ382_000991"
     CDS             complement(15243..16247)
                     /locus_tag="JZ382_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAY2427192.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(16244..18367)
                     /locus_tag="JZ382_000992"
     CDS             complement(16244..18367)
                     /locus_tag="JZ382_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY2427193.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(18372..20819)
                     /locus_tag="JZ382_000993"
     CDS             complement(18372..20819)
                     /locus_tag="JZ382_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2427194.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(20806..21195)
                     /locus_tag="JZ382_000994"
     CDS             complement(20806..21195)
                     /locus_tag="JZ382_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2427195.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(21255..21758)
                     /locus_tag="JZ382_000995"
     CDS             complement(21255..21758)
                     /locus_tag="JZ382_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAY2427196.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(21771..21932)
                     /locus_tag="JZ382_000996"
     CDS             complement(21771..21932)
                     /locus_tag="JZ382_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427197.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         6581 a         4447 c         4078 g         6861 t
ORIGIN      
        1 attgattacg ttaacaataa ttcgtttcat gtgactagat catactaggc atgaaaaatc
       61 tctgaggaac atggtttccc taggaggttt ttcgtgtcta tttttgtttg gccgcaatca
      121 actggtctag caaacgtgaa aaccaaatct tcaaaaaggg tgcgcgcatg aaaatcaaaa
      181 agatctttaa tcaaaatgca gtattggtag atgacggtgg acaggaaaaa gtagctattg
      241 gaaaagggat tggatttgac agaaaaagaa atgatttgat ttttgatcgt gatgtcgaac
      301 ggatatttgt catggaacca gaaggtcaaa tcaagttgca aaatttactt aatcagattg
      361 atgaacgctt tttatttgca gcagaaacga tcattgatca tgcggaaact gttttgatgg
      421 aaaagttgaa tgaacatgta ttgatcgctt tgaccgacca cattgctttt tctgcggaga
      481 acattaataa cggtatcgtc atccgcaaca aacttttaag agaaatcgaa gtgctttaca
      541 gtgatgaatt cagtattgct caatgggcag tagagtatct gacaaaagag cttgggattc
      601 catatactta tgacgaagca ggatatatcg caatccatat tcacagcgga cgaagcggtc
      661 ggaacaataa ccatcgcagt atccgtgaag tgacgattgt atctgacatc atccatttag
      721 tggaatcaga attggaaaca gatatccatt cagaaccatt ttcactgaat tattcaagac
      781 tggtgaatca tttacgactg cttctacaac gaacccatgc gcaacaatat gctgttttag
      841 acacggaaat cgtagaaatg gtcaaacgaa aatacccaga aagctataag atttctaaaa
      901 aaatccgtgt gttgctgacc aaagaatacc agctcgcgat cacgaaagaa gaactgggtt
      961 acttggccat ccatatcgag cgtttaagaa gcgcaatcgt acaaacaaat gttaacaatc
     1021 atgaggagga aaaaaattga aagcatatct acaacgaatg ggacgttccc ttcaacttcc
     1081 agttgcggtc ttacctgctg ctgctttact agtagggatc ggaaactggt gggcatctta
     1141 tagtaacgac attgtcgctc attttttaca agctggcggt aacgcagttt taggtcaatt
     1201 acctttgctg tttgccgttg ggttagcact agggatgtca gtggacaaat caggtgctgc
     1261 tgccttggca ggtttggtcg catttgaagt tccagtaaat gttttaaaaa ctgattcagt
     1321 agctacttta ttgaatatca aaccagaatc agttgatcca tcttttgctc agatcagtaa
     1381 cgtatttatt ggtattattg ccggattgat tgcggcagct ctttataatc gtttccacga
     1441 agcaaaatta ccgatggctt tatctttctt tagcggcaag cgtctagtgc caatcttggc
     1501 tgcctttgtc atgttgctga tcagtgcagt gctactgctc gtatggcctc cggtttactc
     1561 tgctttagtt tcttttgcta aattcatttt gagtttaggc tggggtggtg caggtcttta
     1621 tggcttcttc aaccgtttac tgatccctac aggattgcat caagcaatga actccgtgtt
     1681 ctggtttgac gtagcaggaa tcaatgatat tggtaaattt ttggcttctg aaggaacaaa
     1741 aggcgtaaca ggaatgtatc aagctggatt tttcccaatc atgatgttcg ggcttccagc
     1801 aggagcatat gctatttaca gaaatgcacg tccagaaaac aaagcaaaaa cagcttcctt
     1861 gatgttagca gcagcttttg cttcattctt tactggtgta acagaaccac ttgaattctc
     1921 atttatgttc gttgcttggc cattatatgt cttgcatgca attttcactg ggatttcttt
     1981 agcagtgagt gcattcttcc attggacagc aggatttgcc tttagtgccg gatttgtcga
     2041 ttacttccta tctttgaaaa atccgattgc gaatcagcca ttgatgttga tcgtccaagg
     2101 attagtaaca gcagtgattt actactttgg ctttaacttt gctatcaaaa aattccatct
     2161 gatgactcct ggccgcgagg aagcagatct ttcagatgac gatactgcaa cgactaattc
     2221 agacaataaa tatgcggcac aagcaagcaa aatctacgct gcattaggcg gagccgacaa
     2281 tgtaacttcg atcgataact gtactacacg ccttcgtttg caagtcaaag atacaggcct
     2341 gatcgatcaa aataaaatca aagccactgg cgtacctggt atgaagatca tcgacggaaa
     2401 aaatgcgcaa gtcatcgtag gtacagaagt acaattcgtt gcagatgaga tggcaaaact
     2461 acatggtggt gctgcaacac gacctgcaca aacaaacacc gtggttaaaa cagaaacatt
     2521 tgccggtgga acgatcgaac aagatattta cgctatcgca aatgggaaac tgattccaat
     2581 cactgaagtt tcagatgatg tattcgcgga aaaaatgatg ggtgacggct atgctgtatt
     2641 accagaaaat ggagaaatct tctctccaat cgctggtacg atcacaaaca ttttcccaac
     2701 gaaacatgct gtcggaatcc aaacagatgc tggtatcgaa gtcttgcttc atatgggcat
     2761 caacactgtt gacttaaaag gcgaaccatt cactttatat gtaaaagaag gtcaaaaagt
     2821 cgcaagaggt caattgattg ctttagttga tcttgccgct attcaatctg caggcaaaaa
     2881 cacggatatg gtcgttgtat tcacaaatgg cgacaaagtc caaagcttag aaatcgaacc
     2941 cgcacgtgat gtaaaagcaa atgataaaat cggctctgtt tccaacaaag cctgagccat
     3001 cgtcttcccc agactttttt atcgataaac aaacaggagc tgagacaaaa atcttgtctc
     3061 agctcctttt tccgaataaa aacggcgttc tcctgccaac tccgcggtaa taagtccagc
     3121 caaacgtaac atatgaagag ctatttttta tttgactctt cttattactt gatgcagaac
     3181 agctttttta caacctctga taaacggtgt tcaaaaacca gtttatcggt atgaagtcaa
     3241 atttatgcaa aaatgtggaa agccattttc acaaaatttt tcttcatact caaaactaat
     3301 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
     3361 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
     3421 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
     3481 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaacttt
     3541 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
     3601 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
     3661 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
     3721 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
     3781 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
     3841 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
     3901 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
     3961 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
     4021 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
     4081 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
     4141 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
     4201 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
     4261 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
     4321 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
     4381 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
     4441 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
     4501 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
     4561 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
     4621 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
     4681 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
     4741 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
     4801 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
     4861 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
     4921 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
     4981 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
     5041 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
     5101 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
     5161 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gattacaaca
     5221 agatacgttc cttttgggaa tgggatgatt cgggtagtgt ttggaacatc ctgactcgtt
     5281 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
     5341 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
     5401 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
     5461 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
     5521 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
     5581 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
     5641 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
     5701 tcgactaact cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
     5761 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
     5821 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
     5881 atcaaactgc ccttttcttc caagcgggct ccatcaaatc cataagaaac gttgattact
     5941 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
     6001 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
     6061 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
     6121 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctgctgatt
     6181 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
     6241 tatccatatc taaacgccgt ttctgtcact cttgatccat ctaggagatc gcgattagcc
     6301 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
     6361 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
     6421 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
     6481 atcttcctcc ttgcttgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
     6541 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
     6601 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
     6661 aaattttggg gaagacacat tctaagtaaa caaagcggta aaaatatctt ttattccctt
     6721 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
     6781 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
     6841 tatttctaac aaaatcactt catttttgtc agataaataa aaacgtaaat ttcaagttta
     6901 agttagccac tacaggtaac tcatttgttt gataccgtaa ctgcctcgtt attctaaatc
     6961 agtgaggcct tgataatgaa ctgaggtatg atagtcttca gacgattctg gccccaggcc
     7021 agctttccag acatgaagcc atgtcccgag tgaggatcgt tatcaaggaa taatgggtca
     7081 agccaccaac cgttcttgct tgaatgcctg gacgaatgta tcatgtggtg tcgcataacc
     7141 caaaattttc cgcgggtaat cgttcatcca acgctggatc cgcaaaatct ggttctcgct
     7201 cacctcacta atcgggcttc ccttggggac gaaacggcga attaatttgt gctggttttc
     7261 gctcgtccca cgctcccaag aagcataggg gtggctgaaa tagacgtcag ctacatcttg
     7321 cagagcatcg tgtagcccag aaaactccga gccattgtcg gatgtaacgg tcttaaataa
     7381 ttgatcgaag ttagcgccag cccgttctct tagctgttga accgcttggt ccaccgattc
     7441 ggtgtccttc ccgttcaact ttaggatgac ttcaaagcgt gtctgccgtt cagccagcgt
     7501 cagcagcacg gcatccgact tggtcttgtt tcccactacc gtgtcgattt cccaatgacc
     7561 gaacgtttcc cgtgtttcaa cttctttagg gcgttcttca attgacgtcc cgagaacacg
     7621 cttgttggga cggtgtcctg ggcggaccgc tttgatttta cgggacagtt tttcaagcaa
     7681 gtccatgttc tttgttcgca tgattccgcg gtcaatccat tgataaagag cggttgtgcc
     7741 aggaatgatg gcacaatcga acagctcgtg ttttctggca aatccgacca cgacgtccgg
     7801 cgaccatttt tcatttaaca ttttgtcatc tgcccaatca atgaaggcat ttgtgttcgc
     7861 ccatttgggg cgtctgccac agttcagtcg ttgcttttca tagaaggcct gtcctgcatc
     7921 agcgtcataa acagaatagt aggtgtcata cacttttcca ttttgcgttt ggcgttttaa
     7981 ctgggtgatg gttccgcgat caatctcatt gtttatggtt tgcggaacac gtcccagcac
     8041 tttggcaacc tgacggttgg aatagccttc ctttttcagg atagcgattt ggcaacgttc
     8101 tgagtaggaa agatgttttc ccttgcgtga aactgtgtta tcatgggttt gcgtcatgtg
     8161 aattcatcct tgtctattga gagttgtggt aacttcaata taacatggaa ttcacgtggc
     8221 gtttttttta ttttaaatgg ctaagttcat tataaaatcc gccaatgaaa aaatctttaa
     8281 aacttgcatc agcagttata ttaatgtccc aactccttat agcaacacct ttccatgtgt
     8341 tggcaagcga aaattcaaca tacaaaattg caaaagaaag aaccgagacg actgaaatga
     8401 gtaattccga tgtaacagca gagagtagtg aacttttgac aacttcatca agtgaaagta
     8461 gtacgacttc aaatgccgag tcttcaacaa cttcatcaag tgaaagtagt acgacttcaa
     8521 atgccgagtc ttcaacaact tcgtcaagtg aaagtagtac gatttcaagt accgaatctt
     8581 caacaacttc aagtagtaca agtgaaataa cttcttcgaa agtgacaaaa ccaaaccata
     8641 tagaatcttc aaaaaaggaa gcgccaaaaa cggaaggaca aacaaaaact ttttcgacac
     8701 cacagcaaat agaatcaaca gaatctttcc aatcacaacc aatactatct ggtaatgaag
     8761 aaattcattt tgacaaaaat gaatctgtag aaaattttat tatgaaaatt ggtgagtctg
     8821 cgagaaaaat tggtcagaaa tatgatttat atgcttctgt tatgattgca caagcgatct
     8881 tagaatctgc cagtggtcaa agccagttag ctcaggcacc gaattacaat ttatttggga
     8941 taaaaggaac ttataatgga aactttgtca ttatggtaac aaacgaagat ttgggtaatg
     9001 gaacgcttta tacgacccaa tcaaaattta gagtgtatga aaactatgag gaaagctttg
     9061 aagattatgc caaattatta actaagggaa ttagtggaaa taaagatttt tatgcaggag
     9121 cattaaaagc taatagtaaa acatatcgag aagcaactaa attccttaca ggacggtatg
     9181 caacagatac acaatactat ttgaagctaa atgaattaat taaaacctac gatttaacca
     9241 actatgataa agaagttaaa ggtccacaaa tgagtaagac tggataccaa gtgcctattc
     9301 ataattattc aataaccagt ttgtttggaa atcgtggaaa tgaatttcac cgaggacttg
     9361 atatggcagc tacacagggt gagccgattc atgcagttaa agctggtaag gttgttaaag
     9421 cagagtttca ctactcttgg ggaaattacg tggtgataca acacgaagat ggatcaaccg
     9481 cgttatatgc tcaccagcaa caatacctag taaaaactgg agataatgtt ttgcaaggac
     9541 aaataattgg ctatgtagga agtactggga atagtactgg cccacacctt catttggaaa
     9601 tttgcaaaga tagtagtctt tctcagagca tgttagttga tccgaaaacc ttattattcg
     9661 gagactaaaa aaggtaatca atctgacaaa gtaacaagaa aatggttgga ttcttagacc
     9721 tttgatcagg atattgagac tggaattgat atctcgatta cgatgggttt gacaaataaa
     9781 acaagtccat tatcgaatat cgagtgtatt attgccgtct ttatgctcat attttgagca
     9841 ggtttcacta gatgaaaaca atttatctat cttgaggatt tcgtgtacgt atcgttcagt
     9901 cttgtattgt aaattagtca taaaatcaga ctaagaaaaa ttggaaatat ttttttctga
     9961 tttattaaaa gttaattgaa attatatggt acttctttct tgggagggta ggtgcagtga
    10021 aggaaagata tggtcacctt actgttgttg aggagattac tcataatgga tataaaaaat
    10081 atcgttgcct atgtgactgt gggaatgaaa cgattgtata tcgatcaaac ttactatcag
    10141 gtagaaccaa aagttgtgga tgtggaaatt ataaaaatag gcataaatat aaaaatttga
    10201 caaataaaac ttttggtaag ttagttgcaa tcaagccaac taaactgcga agtgaaggaa
    10261 ctgttgtttg ggaatgtcaa tgtctgtgtg ggagaaaaaa gttagtatcg gcaaagcgac
    10321 ttctaaaagg tgatgtaaaa agctgtggat gtttgaaatc aaaaaagcat cacattgaaa
    10381 ataaaaaatt cggattactt actgcaaagt atcctttgga caataactgt tcatggaaaa
    10441 caaaatgggt ttgtcattgt gagtgtggca gtatgtgtga gataagttat agtaacttat
    10501 tttttggaca tactcaaagt tgtggatgtt taaaaaggca agaaacaagg acatgtgttg
    10561 aagggacagt aatagagtgt ctaacgtcaa aattaccgaa aaataataca agtggtgtta
    10621 agggagttta tcgttctaaa gagaaataga ttgcgtatat aactttaaaa aaaaagaggt
    10681 actatttagg atcgtatgat tctattaaga gtgcagctca agctagaaaa gatgctgaaa
    10741 ataaattgtt tcaacctttg attgacaaat atcgaggtaa tcattaatta ttgtggataa
    10801 taattaacag tgatatataa acatctataa tatattattc atactattga tatttagtat
    10861 aatcctatgg aattgaatat catagcaatg tatttgaaaa gcaaattgga aaacaaatag
    10921 gtgcaacacc ataaatatga aaaaattggg aaaaagtaga atggaaattt gcttacgtct
    10981 cacgagatag ctcacactat taatctgaag aacaatgaag tcgactttta gaaattgcta
    11041 gaagaaagta aagcgaatta tcactagtta ctatagagta agttcaaata aagcatctcc
    11101 aacttacatg gttacatgag gatatcaaat cgaaattact caagcttcct atggctagag
    11161 tcgcgagaag ttgttgggca gtagacatag gttatattgt tcttagctac acgatcatta
    11221 gaaaggaaat cctattatgt atagttatca agaagatgca aaagtccaaa aaaatcgctg
    11281 gtggattttg attgctatat ctatgtttac cttcatgtcc acattggatg gcagtattgt
    11341 caatattgca ctacctacca tctcagatga tatgggcata ccaatgaatc aagcggaatg
    11401 ggtagtgtcc gtttatctga tgattgtctg ttcttgtttg ttgctgtttg gtaaaattgg
    11461 cgatagccgt gggaaaatca aagtatttcg gattggtacg ctgatctttg tcttgggatc
    11521 gctgttgtgt gggttcaatc attcgctagg tattttattg ttcgcacggg tggtacaagc
    11581 cattggtgct agtatgacta tggctacaaa tgccggaatc atcacggaag tctttcccat
    11641 acatgaacgt ggtcgggcat tgggagcaat aggtgcgttt gtttcattag gctcgattgc
    11701 tggaccaggt cttggcggct tgattttgtc ccaattttct tgggcgtaca ttttttggat
    11761 caatgtaccg gtgggaatcg taacgatgtt gattgctgaa aaattcttac caaaagatat
    11821 caccatgagc ggccatacaa tcgatggagg gggctttgct gccattgcgg ctacgattat
    11881 cagctttttt ggagcaatct ttttggggca agaaagtggc tttttccatc cagtccccct
    11941 tgtactgttc gtaatttcag tgattgcctt tgtcagcttt ttaaaagttg aaaagaaagc
    12001 agcctctcca ttgatttctt tcggtatttt taagaacaag atttttacca tgagcttgat
    12061 tacagccttg ttgatttttt cttccaactt ctttgtcaat gtagtgattc ctttttatct
    12121 gcaaaatgct cgcgggttga gtcctagcta tgctgggctg ttgatgatgg tctttccttt
    12181 gatgatggtc gtcggggcgc ctttaagcgg ctacctgacc gaccgggttg gcccggagat
    12241 tttggtatta gtaggcttgg gcttattgtc ggtaacccag gtcatgtata tgtttttgtc
    12301 agtggactct cctttgtggc tatacattgt cgctactgcc atcatgggtg ccggcaatgc
    12361 cttgttccag tcccccaata atacgatggt tatgagctcg gtcactatcg aaaacttggg
    12421 actggcaggc tcgatgaatt cctttgcccg aaatttgggg atggtcagcg ggatttcgct
    12481 ttctacgacg gtcttatatc aagcgatgtc tgctaagatg ggccagcaag taactaacta
    12541 tatcgcaaat cggccggatg tctttttata tggaatgaag attacctttt taggcagctt
    12601 tgttttgtgt tttgttgcct ttttcctgac tatctggcgt atcaaaaaac aaggaggtat
    12661 tcagaaaaat gtctgaaaaa accactgttg aagaattttt ggaggcctgc gtatgggtga
    12721 gtgggatttc catggatgat ggaagatgaa gtgaacttct aaaagccgaa aaactcaagt
    12781 attatcttca aaatactagt gctcaatgag atgcttgatt tgatcacaag aagactgcga
    12841 aagccgctgg caaccgcaag ctaggtatcc caccgatgga ctacgtttta gaggctactg
    12901 gttatgaata cggagccaac gctctcatct gcataagaat acatcaccca gattatgtaa
    12961 agattttcga tacttctgtc aaaaaatcat taagatagat aaatgatttc cgtctagtca
    13021 aattggttca gaatgtagac acacagatgg taagaagtca ctcacaattc gagaatggac
    13081 ttgtcctatt tgccgtatac atcataatcg aaacgtcaac gatagtatca atattttgac
    13141 caagggctat gaatacaagc attgccttag aaaacatcaa gaaccttagg aacaacgggg
    13201 atagcttggt taataagaga taccgctgtt taggcttgca agtatactct tttatcaaga
    13261 attccgtggc tttagtcatg agaggcttaa tactatgaaa attttcttta gcatttttga
    13321 tttaggttcc ttttctaatt ataataagat gaccactaca gtaacctact gaaatatata
    13381 aacagccact gtagtaccat tttcaaagta gtctatgaga ggagaatctt cattcttatg
    13441 aacgctggaa ttgcggatat gaatttaata aaaaaaaccc ttaatgattt tacttccaat
    13501 tcaatatcaa aaggtactgg tatcaattta agcacaataa aaaagctgaa aagtggtgaa
    13561 agatcagtgg aaaagctgaa tcttttagat gctataaaaa taacggaatt tgctatgaaa
    13621 aatggaaaag ctgagattga aatatggaga taattatcgt ctagtgaatt acttgccact
    13681 tagctgaatt taacgatcct tggtgcttga agggggagat tcttgggaat agagcatact
    13741 tgcaagccta tgtctttact aacaacgata tctcttatta accaagctat ccccgtagtt
    13801 ctaaggcttt tacttttatc taagcaataa tcgtattcaa aggaaatttt tgacccttag
    13861 aaagtctcat tttatcagac gtgagcttag gcattgctga attttgttaa ttcggtttta
    13921 ggttgtgctt tgagcataat atgaaagtat tctttatcac gatttcattc atctaaggta
    13981 atgtgatgtg attctgaaat tctttcaaag gcagtttttg cataagctga cattacatca
    14041 tcaatgacat gtttacaatg tttcactact aaaataaggg ggtaaccaag aagaaaaact
    14101 gaatgattat ttgtatctaa ttccatggta tatcaaccat ttcctatata gactgattat
    14161 gacatggatt aaaataaaaa ggcactatgt cttaattgtc ggttttcgag ttaccgaatg
    14221 gcattcatca cccacctata gaggatgggc gacttctgcc tgaaataagt tacactactt
    14281 ataacttcta ataaaattag attgagtcat tattatttaa catcaacgat tttccagttt
    14341 ccattttttt gtaatactaa tttgtactga aagtagctag tggtttttga tacattatca
    14401 aggtatttta tctttgtact cactagaaaa ttttttccgt tttgtagata aactggattc
    14461 tctaactccg aaaactttaa gtcaatattg attggtcgca tcgcaccatt ttcaacataa
    14521 tactccaatt ctttttcaga agctgatgga taaagtttaa aaaatgtttc taaaaaatcg
    14581 gtgatttctt ccgttgtttt ggcatcaatt tctgaatcgt tttctacttg ttttgcttca
    14641 tattttgcct tgtctggctt tccaatcatt gttggcaaac tagttactaa atgattcctg
    14701 ttttcatctt caaagattgt cactcgatat gcagaagaaa ttgattgcgt cttttttccc
    14761 tctgtaatat gttgctccac taaaaatgaa acgacaaatt cttccgagtg tttttcaaca
    14821 tctagaattt ttacagattg aacttcggaa ctcgtgggaa tatctgctcg aaccatatct
    14881 tggattaaag cgagaccgtt ttctgtaagg tactgttcga gattactcat tctcttttca
    14941 attgcttctt tgttattttt ccatgaaaaa tattctttcg caaaatcctc tgtaaaactt
    15001 tcaacgccag aagtattcac taccttctct tttataacca ctttttcatg agttgtatgt
    15061 tgatcaatgg cggtgaaatt tttataaacg ccaaaagcaa gactacaaga caataaaagc
    15121 caaagggcaa gcaccatttt tcgatgctta cccacggata caactcgttc ttttttcttt
    15181 tttgggctct tttccttttg attccgttca atttttatct tcattctttt tcctcttttc
    15241 tcttatttgt atcggccagc tccgataaga tgctgccgcc aatagttttc cgttagattc
    15301 gtccacccca ctggatctcc tgcatgaaac atgcgattct cccctgcgta aatgcccaca
    15361 tgggtcacat aagtaccggc atcataggtt gaatgaaaga aaactaaatc acctggtttg
    15421 gcatctttta gatcaatatg agtcatggca tcatattgat cctgagccac ccgaggaagt
    15481 ttcaggtcaa ctttggcata acaccattgg gtcaagccac tacaatcaaa ccccgtttct
    15541 ggacttgagc caccaaacac ataggccgtt ccttcatact taaatgcctc atcaaaaatt
    15601 tcttggacta ttttgtcatc aaactgagtt gtagttaggt attgcttcac taataaacag
    15661 taaaacatat tgccataatt ataccgccac ccaccatttt ccttaatcgc gattgggttg
    15721 gggtaatcga ctttttgacc acctgatttt tcctttgaaa aagattcagc taaagcaaaa
    15781 ctatgcttct tcccgttttt cccgacatga tctagatacc cgccaccata gttataggcc
    15841 tgaataatac tatcttgatc acaccctagt tctttggaac gcttaaccaa agacgcaaaa
    15901 tagagacagc cttgtttgat ggatgcctcg gtacttaatg aattaggtgg taatccggct
    15961 gactcagaac tttgcataac gtcttccatt gtgccaccag attccacttg aataatcgcc
    16021 aaaagaattg gaatatactc tgttatatcc gattctttgg catacttttc caccattgca
    16081 cggtgtttca atacttcttc tgagacacta gttccgccag gagaagtgcc agaactacca
    16141 tttccctcac tgtcttcacc aaacaacaaa cccacacaca tcaaaatacc cataaaagag
    16201 atggtcagaa aagttaatga aataacaccc actttcttca agttcatttt ttctcacctg
    16261 accttgattt tggacgatgg ttcattcttt tttgtacttt ttgattggtc tgctttggca
    16321 gagtggacct ctgtttcact tgttggaaag aatttttctt acttgctgat tggcttttcc
    16381 cttttgaaat gatttcttga ctagttagtt ttcgtttgat ttcgggattc agatttgctc
    16441 tcacaatccg ctttggtgct tcttttttca gcatcatatc tttctgaggt tgagctgctt
    16501 tatctattgg aatattcgcc cttctttttc cctgacgatt tgttgcttca ctcagtatct
    16561 tgcgtttttc gtccattttc gcatttcttc gttgtcgttg tttttctcgc aaatccactc
    16621 gatccgcttt ttcttggaac agtccgcgct tgaattcttc tggtgctttt ttcgactttt
    16681 cgattccttt atggagattg tatttcaaat tcgttggcgt attccttacc tgatccttta
    16741 cttgtttagt cttatctttc accaaagctt gggtatccaa aactttgccg atttttctcc
    16801 cagccaaatt cattcgggat tgtttcttat tatttgaaac tggattctcc ttagttaagt
    16861 cagatagtat ttcgtgatcg ttcggtaatc gtgaattctt tcgtagagaa gaaccggatg
    16921 gcttaaattt ggattttgat tgttcctttg ccaacttatt tccgactgct gcaccaccac
    16981 caaaagccaa agctcgtccc acatttcgat tgagttgccg caattttcga ttcattaaca
    17041 tttgcggttt tcgcatgacg cgtcttccca ttgactggct atcattactc tggaggttga
    17101 acatactcat aatgtcgccc aatttgaaat agataccggc aaaggtcaca atttgcagaa
    17161 aagcaaccat gaaaaatgga taggttgccg aaatattgaa aaacatcgtg gagatgctaa
    17221 atgcagtcgt aatcaccaaa gtaacacccg ctcgcatcat aatcgtattg aataaacgaa
    17281 taatcgcctt ctttcctaaa ctttcataag tcggcaacat ggacaataag aaactaattg
    17341 gcagaaacat cgcaaagata atgaagagaa tttgcgagaa caacataatc cctgttagaa
    17401 gaaaaacaaa gaaggaaatg ccaatattga acaataccaa aaagaccacg gtccctaaac
    17461 ggttcatggt cttggtaata ctgagattcg cattgtcatt gtcttcaatc tctgccttga
    17521 ccgcttcttc ccgatctttg cctttgtttt catctggact cacagataga atcttgttga
    17581 cccggtcttc accaatttct tctatgttgg aatcatcaaa ttgcagtagt aaccaaggtt
    17641 gttggacttg aatcgaaaac aaactatccc gaatcaaatc cacactatct tttccttggc
    17701 tttctgaatt gggtacaacg atctcggttc caagggtaag ggccgcttca ctaacatctg
    17761 agctaaagtc attgattttt gtgatatagg taggcgcata agcaatgaac gaacccgata
    17821 gtagaaaaat catcacaaaa ttaatcacgg ctcgaactgc tttcgtggtt tctcgtttta
    17881 ataatccggt atacgccaca tacaccccca gagctaaaat catcaataat agaaacccaa
    17941 aataaaatcc ggaggtctgg agtccatttt cagtaatacc agccaatgtt tggatattct
    18001 ttccaatact ttcagccgta tccgaaatga aatctaattt gtaagcctct tgaaccacat
    18061 aacccgtggc gttcgacaaa tacaaactca ccgtccaaat gaaattagta atggcataaa
    18121 gaccatacat cacgcttttc cctaagccat caccccaatt ccaaggcaac caatcccaag
    18181 agctatccac aaagaaatct agttgatagt tgttcagtga atatttggaa tatagatttc
    18241 ctgcttcaac tgtatcatca actagccctg tcgcctcggc taccgtacca actaaactca
    18301 atagaactat cagagtgagt atgctaataa aaacgattcc cacaatccga aaaagtttct
    18361 ttttcatccc ctcactccat tctctcttga acaggtggac gggtatcaaa ggcatggaag
    18421 agttcttcaa agactggatg tacctgaacc acgcctaccc gaccatacaa atcctgcatc
    18481 aagcattggc cattttccaa atctcgtagg cgtttttgat tgctttcatc ttctcgatcc
    18541 aagccaaaga attccaaggt tttcttgatt tcatgaatat cggtactgcg aaacgcaaac
    18601 ttcaacccga tattattctt caagcgttca tccagtaaat catcactatt ttgcgtgaca
    18661 aagtaaaccc cggcattcat agcccgccca gcacgaacga gcttattcga taatgttttc
    18721 ccttgcgcta cttgaagaaa actccacgcc tcatctaagt ctacaatttt gaagatttct
    18781 cgattactat ggataaagtc caaggcaaaa gtggaaatga cgattagcat ggaaacacta
    18841 agcaattcca tggtggtata ttcttcaaaa gtggtttctg catctggtaa aaccaaatcc
    18901 gctacttgaa tgatatttaa ctgcctatct aaactaatcg actgggtaat cgaaccatct
    18961 gagaaaagca aatgggcaaa atcataatcg gtaaaggact cgatgtgatc cgcgatattt
    19021 tccgcaactg gtgagccttc ttttcgaagc tcattaatta cttgtaataa tccccgttga
    19081 ttgctttggg taaccgtccg aatggcctta cgcaacactg gaaacttctc cccatctcga
    19141 ctagaaattc ctgttaaaaa ggtcagaata tcaattgcta gactttcaga atccttcttt
    19201 ttctccataa tcacataagg atcaagtaat ccttgatttt ttggcttact agttaagttg
    19261 agaatattga tttcatccgc aatttctggc aaggtttctt tccagccacc ccgttcccct
    19321 tttggatcga caatcacagc ttgtccacca aaaaggacgg cgtaataaac taagagattg
    19381 ttactaaagg actttccgcc accgagtgaa ccgaggaatg ccgctgctag cgcattggta
    19441 actgaatcct tgaccccttg tgaggccaat gccggtttca ggtaaacatt gcgccccgta
    19501 tctacgttat aaccaaagta aatgccttcc aactccccta acatctgagt ggcgccaaaa
    19561 ccaagtccgg cgagaaaatc agacgtaaca tactggatat aatcattcat gtatcgctta
    19621 ctagaaggta agaattcttc atgtagcccc atcatatcgc caaaaggccg gactagtttt
    19681 acattggtat catcataaaa atctagcact tcatcacacc ggcgctttag ttcatctact
    19741 gattcggcac ttactcgaac cacatagctc agtttgtaca tggattcttt ggactgatcc
    19801 aaggtagttt ccaattcatc tacggaatcc aatgcatcta agacattgga atttgtttca
    19861 ttgtcagatt gataggcatg gttgtccaag tcttttaatt cctttttctt attgcgaacg
    19921 gtagcaagtg cctttttgtt cgtcacaatc tccacattca tactagtgtc aattgggaat
    19981 gtaaattgtt gttgctggta atagaaaagt tctgaggagg gaaactccat ttcccccaca
    20041 attgtattaa ttgttaagta cgctacatat gattcatgat tctcatgttc catgcataag
    20101 taccggggct tttcttcaat caaacaacga ctgggacgta ataagtcgta acgtttcacc
    20161 aaagtttccg acttcagctt tttctttggt agctgaaatt catattcttc aaatggtgta
    20221 cccttttcgc catagatatg ttcaatcaaa tacatcagat cactcggtgt gactcgtctg
    20281 actttaaatc ttcgagccaa tttactttcc attagttttt caagcttggt atatcggcga
    20341 atttcttcat tggataagga aacaaaatcg cccatcaaat gatgattcac tccatagaca
    20401 aattcttgaa agccggagaa gaaagatttc ttgagattct ttagattgac ctcttcatct
    20461 gtcgcaatca gtttgaagcc aataaaaaag cgataatcaa tctgactatc gccaatcatg
    20521 gatactaatg cctctgtctg taaatcaatt cgttgtttcg ctacttctgt taatcgcccg
    20581 gtaatttcct tttttgatcg ttcttgcgtg gcacgaatac tactttctgt ggcaatttgt
    20641 aaggcatgaa tcttgccttc acgattttgc gcaatcaatt gacgaaagtt atcgtggact
    20701 tgatatttct gttcaggtga caaaaaggag taattgtaag gtattaactc atagtaggca
    20761 aaacattctc cctcttgatt aaagacaaga ttattttcaa tatacttaat cgggtacata
    20821 gttcacactc cttaccagag taatgggttc gtcccatcgt ttcttctgtt ctttgacttt
    20881 ctttcctgcg aaagtgacct taaatcgtaa gtgatagcta ataaaagatc gtaaaaatcc
    20941 caatggtttc ttaccgtcaa acgttttttg cgacatgaac caagtaactg ccaccggtat
    21001 acctaagtat tttaaaaagg ccccctctat catagatagt ggaggcacat gggcaaatac
    21061 gatgacaaac agcaaggata aaacaaacca agccatctgg ttaaaagtca caggaaaagg
    21121 cagttgaaag tcattaattg cataaatgac cttttcaact gcccaaatac tggtgtagct
    21181 ttttattttc ttcatcaaat cattccttta aaaagaagga tagtttagaa atcaactacc
    21241 cttcaataat tttgttaata tggatactca aaaacacctc gactcgttac caaataattg
    21301 cctccaatct ccaagtctct accacacgca tcaaaatcaa tatacgtctt caagtgttct
    21361 ggtacttctc caaaatcacc agcttctaca atcaagaaat atgctacatc actcatatca
    21421 tcgcaatctg gataatagat aatatcatcc acatgctcta ccatctcctc aaaagaattg
    21481 aagaaagcat gttgaatctc agaagcaact tcaccaactg gcgttccttc aagttcctcc
    21541 gctaagccac acaaccgatt aatttcctca attggcgtat actcatcaat ctcaaaggga
    21601 agttcataat catgaattgc atattcctcg tattcgtcat ttaacccaat tcgttctttc
    21661 acttcatcaa aatcgacagg tggggtaaac cacgccccca ccaattcacc ttcgttatat
    21721 ttcccgaggt tcgcaatata aacacgcatc tgttccatcg tgcttccccc ttacgcaccg
    21781 acaattcggt taaacaaact gagtaagaca tccttcaccc cagaagcatt aaagaccaat
    21841 ccgaccgcaa tcaaagcaat caccaaaaag ccaatcagtt tggaaaactc ccgcttaaat
    21901 cctaaaaaca aaccaatgac cacaattgcc agtaagacta aggattgggc attggttaaa
    21961 aaccatt
//
LOCUS       DADJYG010000032        21726 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.032, whole genome shotgun sequence.
ACCESSION   DADJYG010000032 DADJYG010000000
VERSION     DADJYG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.032"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(205..1104)
                     /locus_tag="JZ382_000997"
     CDS             complement(205..1104)
                     /locus_tag="JZ382_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY2427198.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="JZ382_000998"
     CDS             complement(1114..1470)
                     /locus_tag="JZ382_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAY2427199.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="JZ382_000999"
     CDS             1825..2121
                     /locus_tag="JZ382_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAY2427200.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="JZ382_001000"
     CDS             complement(2765..3718)
                     /locus_tag="JZ382_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427201.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="JZ382_001001"
     CDS             complement(3741..4496)
                     /locus_tag="JZ382_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2427202.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="JZ382_001002"
     CDS             complement(4493..5452)
                     /locus_tag="JZ382_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAY2427203.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="JZ382_001003"
     CDS             complement(5445..6395)
                     /locus_tag="JZ382_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAY2427204.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="JZ382_001004"
     CDS             6545..6997
                     /locus_tag="JZ382_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAY2427205.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="JZ382_001005"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="JZ382_001005"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAY2427206.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="JZ382_001006"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="JZ382_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY2427207.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="JZ382_001007"
     CDS             complement(10549..11358)
                     /locus_tag="JZ382_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427208.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="JZ382_001008"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="JZ382_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAY2427209.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="JZ382_001009"
     CDS             complement(14968..15654)
                     /locus_tag="JZ382_001009"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY2427210.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="JZ382_001010"
     CDS             complement(16002..17783)
                     /locus_tag="JZ382_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427211.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="JZ382_001011"
     CDS             complement(17809..18723)
                     /locus_tag="JZ382_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427212.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="JZ382_001012"
     CDS             complement(18750..19712)
                     /locus_tag="JZ382_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427213.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="JZ382_001013"
     CDS             complement(19712..20656)
                     /locus_tag="JZ382_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427214.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="JZ382_001014"
     CDS             complement(20657..21661)
                     /locus_tag="JZ382_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427215.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6069 a         4638 c         3846 g         7173 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaat
//
LOCUS       DADJYG010000033        21721 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.033, whole genome shotgun sequence.
ACCESSION   DADJYG010000033 DADJYG010000000
VERSION     DADJYG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.033"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(111..1037)
                     /locus_tag="JZ382_001015"
     CDS             complement(111..1037)
                     /locus_tag="JZ382_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY2427216.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1312..2145
                     /locus_tag="JZ382_001016"
     CDS             1312..2145
                     /locus_tag="JZ382_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY2427217.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2179..3015
                     /locus_tag="JZ382_001017"
     CDS             2179..3015
                     /locus_tag="JZ382_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427218.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYHILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEIE
                     MEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFGL
                     IY"
     gene            3041..3601
                     /locus_tag="JZ382_001018"
     CDS             3041..3601
                     /locus_tag="JZ382_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAY2427219.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3800..5521
                     /locus_tag="JZ382_001019"
     CDS             3800..5521
                     /locus_tag="JZ382_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAY2427220.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5686..6828
                     /locus_tag="JZ382_001020"
     CDS             5686..6828
                     /locus_tag="JZ382_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY2427221.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6844..8055
                     /gene="thiI"
                     /locus_tag="JZ382_001021"
     CDS             6844..8055
                     /gene="thiI"
                     /locus_tag="JZ382_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY2427222.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8628..9275
                     /locus_tag="JZ382_001022"
     CDS             8628..9275
                     /locus_tag="JZ382_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAY2427223.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            9668..12313
                     /locus_tag="JZ382_001023"
     CDS             9668..12313
                     /locus_tag="JZ382_001023"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY2427224.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12623..13936
                     /locus_tag="JZ382_001024"
     CDS             12623..13936
                     /locus_tag="JZ382_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY2427225.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13926..14579
                     /locus_tag="JZ382_001025"
     CDS             13926..14579
                     /locus_tag="JZ382_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAY2427226.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14634..15185
                     /gene="radC"
                     /locus_tag="JZ382_001026"
                     /pseudo
     CDS             14634..15185
                     /gene="radC"
                     /locus_tag="JZ382_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            15316..16401
                     /locus_tag="JZ382_001027"
     CDS             15316..16401
                     /locus_tag="JZ382_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2427227.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            16398..18290
                     /locus_tag="JZ382_001028"
     CDS             16398..18290
                     /locus_tag="JZ382_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2427228.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            18297..18674
                     /locus_tag="JZ382_001029"
     CDS             18297..18674
                     /locus_tag="JZ382_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2427229.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            18828..19610
                     /locus_tag="JZ382_001030"
     CDS             18828..19610
                     /locus_tag="JZ382_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAY2427230.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(19736..20467)
                     /gene="erm(A)"
                     /locus_tag="JZ382_001031"
     CDS             complement(19736..20467)
                     /gene="erm(A)"
                     /locus_tag="JZ382_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAY2427231.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(20525..20612)
                     /locus_tag="JZ382_001032"
                     /pseudo
     CDS             complement(20525..20612)
                     /locus_tag="JZ382_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            20976..21638
                     /locus_tag="JZ382_001033"
     CDS             20976..21638
                     /locus_tag="JZ382_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY2427232.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         7463 a         3500 c         4477 g         6281 t
ORIGIN      
        1 aaatttacga aaattgtttc tcaaattttt gtaaattttc gacttagttt tcgaagaagc
       61 tatttttgtc acagcctctc tttttaagtt tagctattag ataatgatgt ttagttttgg
      121 ataaacgaaa tcaattcttc acccgacaaa ccatagttta cagcaatcaa tagttttaaa
      181 cgtgcttttt gactattaat actgttacag aaaataatcc ccattttttc tagttctttt
      241 cccccgcctt cataatcata aacaggctct gcgattccat tgaaacaacg agaaacaagt
      301 actacaggga ttttttggac aagaagcttt tccaaagcag ctaaggcttg tggagggaga
      361 ttccccgcac ctaaagcttc gattaccaaa ccgtctactt tcgtatgggc gatggcttcc
      421 aataagtccc cttgcatccc agcatatgct ttaacgatcg ggatagtccc atctacagcg
      481 gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga ataaaattcg attcttagtt
      541 actaagccga tgggacccaa tgtaggtgtt cggaaagtag caacatttgt ggtatgggtc
      601 tttgtcacgt agcgtgcaga atggatctca tcgttcatga ccaccaaaac ccccttatct
      661 agcgcttctt cacaacttgc gacacgaatc gcactttcaa aattataaag gccgtcactt
      721 ccaagttcat tacttgaacg cattgcccca gtcaaaacaa ttggtaattt gttgccaatc
      781 gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg taccgtgtgt gatcaccact
      841 ccggatatac ctgatgcata agcggacaag atacgttctt ttaataccaa catgtgagaa
      901 ggcatgatgt gtggtgaggg aagattgaag atatcttcaa ctaataattc tacatgcttg
      961 gggattgtga tttctgcatc taataatggg tttgccacat caggactaac accgcccgtg
     1021 agcatgtttt ctttcatggc gatcgttccg ccggtgtgta gtacaagtat tttttttcat
     1081 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     1141 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     1201 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     1261 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     1321 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     1381 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     1441 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     1501 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     1561 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     1621 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     1681 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     1741 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     1801 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     1861 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     1921 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     1981 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     2041 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     2101 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     2161 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     2221 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     2281 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     2341 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     2401 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     2461 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     2521 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     2581 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     2641 gaaacgttag agatattaaa agaaccaatt tatcatatat tgctcagtcc ggagtcagaa
     2701 cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa tacgtatact
     2761 gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt cgcaaatgct
     2821 gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga tatcgagatg
     2881 ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt aaaacatgct
     2941 gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact gaaacacttc
     3001 ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag agacaccttt
     3061 tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga tacccacggc
     3121 ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt
     3181 tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc tgctgcacga
     3241 agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa gtgatccgct
     3301 ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt ctttttcatt
     3361 actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata tggggaaatt
     3421 atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga aacctgaaaa
     3481 ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac gtcagattca
     3541 agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg aaaaagagtg
     3601 atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga ttttcgttta
     3661 agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt ttgcacgaga
     3721 gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa gatgcatact
     3781 gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat
     3841 tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa accaagatcg
     3901 attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga ttgaagaagt
     3961 cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc gtgaatggag
     4021 tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc aaattttcga
     4081 agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc
     4141 agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag gtctaaagga
     4201 attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg acgtctatga
     4261 agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc tttttgaatt
     4321 gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt taaacacttc
     4381 tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata cgtatgaagt
     4441 ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca ctttccctga
     4501 acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat atgtattccc
     4561 agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa attcattagc
     4621 caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt
     4681 gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat atgtaaaaac
     4741 taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc gtcaattatt
     4801 gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt
     4861 acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga tgattccaca
     4921 gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa cagtcttcaa
     4981 agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac atgaattgag
     5041 aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat tgcgcagttt
     5101 gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc tagaattctt
     5161 ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa
     5221 catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat tattagataa
     5281 gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc aatatgcaaa
     5341 tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct actatttatt
     5401 caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat tagagcgagt
     5461 agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag acttagtata
     5521 aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt tcttgtcttt
     5581 ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa agcaccttgg
     5641 acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat atattttgat
     5701 aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa aaccagtcag
     5761 cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa ccggttgttg
     5821 cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga aatctatttt
     5881 acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat cgaaaaaaga
     5941 gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag cgagacagca
     6001 gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa acgcggattt
     6061 gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt ctctgttatg
     6121 gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga tcttttggat
     6181 gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa agtaccattc
     6241 tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa attccatggc
     6301 ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc attactgaca
     6361 ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg tattgtggct
     6421 atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga gcagacacaa
     6481 cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac gattttttca
     6541 gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat tcgcggcgaa
     6601 gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag tgcctgctcc
     6661 tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa tgatctggct
     6721 acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt cgagcaattt
     6781 atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt
     6841 cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa aggaaagaat
     6901 cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa agactttcca
     6961 gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg cgcagacggg
     7021 cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc tccaagtatt
     7081 cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat gaaagaaatt
     7141 tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca atttgaattg
     7201 acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt cccgcatatc
     7261 caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg tgatggcgct
     7321 tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac aagcggaaaa
     7381 ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt tgccatgaag
     7441 cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag tgaacaagca
     7501 ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac gatccatttt
     7561 atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat
     7621 tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt
     7681 catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca aactttgcaa
     7741 agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt agcgacaatg
     7801 gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct ttctatccag
     7861 ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg ccctcaactg
     7921 gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca acgagcacta
     7981 gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac tgaagaattt
     8041 tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta gagaaacaaa
     8101 acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga ttagctgatt
     8161 gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac tgaaaaataa
     8221 aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga atttcccatg
     8281 aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa
     8341 ttgcttttca aatttttgtg aaatttcggc ttatttttcg aagaagttac ttctggaaca
     8401 ccgtgtatca gaggttgcga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     8461 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     8521 caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag
     8581 aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa
     8641 cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga
     8701 tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa
     8761 ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat
     8821 atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag
     8881 aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt
     8941 ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca
     9001 atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag
     9061 caaaaaatgt gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg
     9121 atgaaattgt caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg
     9181 tcccagctta tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt
     9241 actttgatga gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc
     9301 ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt
     9361 cacgaccact tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa
     9421 gaggagtaac tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg
     9481 acaaccagtg aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg
     9541 tgatcgttaa ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc
     9601 gtaaatcata cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga
     9661 aaacagcatg acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc
     9721 tggacgttat cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc
     9781 aaaaccttat tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca
     9841 tgcttgggat acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga
     9901 tactttatgg ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc
     9961 gaagcttaga gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca
    10021 agtgtgggaa tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg
    10081 cttatcttta gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt
    10141 acgtaaagtt tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat
    10201 caactgggat cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat
    10261 cgaaggcgcc ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat
    10321 tgcgacaacc cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga
    10381 cgagcgttat caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat
    10441 cccaatcatt gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac
    10501 tccagcacat gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa
    10561 tgttatggat gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg
    10621 ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat
    10681 cgatacgatg atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg
    10741 gctgtctact caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca
    10801 agaaacagat caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg
    10861 gatggaaaat gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc
    10921 tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag
    10981 tgaaaactgg gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc
    11041 tttctcaaca atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac
    11101 tagcacttta gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca
    11161 aagcttggaa tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg
    11221 tgacgaacaa gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt
    11281 aatcgaaaag tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg
    11341 acaagatgtc cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat
    11401 ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga
    11461 tttatctgga gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat
    11521 cgaacgagta actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta
    11581 taactttatc tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta
    11641 tggagatgat gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca
    11701 gatcctacgt ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat
    11761 tcctcatcag ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga
    11821 tgatgaaaca gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa
    11881 tattcgttca gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa
    11941 cgatgctaaa atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa
    12001 tcccgaagag ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt
    12061 tctgacaggt gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat
    12121 caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa
    12181 actttctaat gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc
    12241 aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg
    12301 tactattcaa taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta
    12361 gctgattgaa catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag
    12421 gagatgtgac ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa
    12481 ggagggaggt ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct
    12541 ctgtttcttt tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa
    12601 aagagaaatg ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg
    12661 attaccattt ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg
    12721 gcatccagag agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac
    12781 tgtagcttat ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc
    12841 accttatatc gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa
    12901 tgatttgatc cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga
    12961 agcagtagct ggtattactg aattcgagac attaacagca atggcatttg attatttttt
    13021 gatgaagaaa gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac
    13081 caatgtagct gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat
    13141 acttggagat acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt
    13201 tccagtagta acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa
    13261 agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc
    13321 tgatgagatg tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt
    13381 aaccactccg cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta
    13441 ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt
    13501 gaagaaatct cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt
    13561 agatggtgcc cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt
    13621 caaaggatat caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat
    13681 gctagaacga ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc
    13741 aaaagccatc gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc
    13801 attgtggcaa ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt
    13861 tgtgaccggt tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg
    13921 agatcatgaa gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt
    13981 tagtgtatta cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag
    14041 acctgtattt aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga
    14101 ttttctcgga atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa
    14161 cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct
    14221 cttttttaga agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca
    14281 ttgaactttt attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag
    14341 aaaatgttca acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag
    14401 gtacagcgaa gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt
    14461 ctgcggctga gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga
    14521 tcaaaaaaac agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa
    14581 aatccatctc ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa
    14641 aacgattgat caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg
    14701 gcgcggaagc tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc
    14761 cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt
    14821 tacgacaggc tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa
    14881 tcgaaatcaa agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg
    14941 ctccaccggt tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc
    15001 acaaacaaga acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa
    15061 aaacagtgtt catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt
    15121 atgctgtacg ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg
    15181 ttttaaccat agtataataa ataatagtag ttaaagtagt ttaagaggag atattaaagg
    15241 gatattttac tttaaataaa aattaacaag gtttataatt tgtagtagtt aaagtattta
    15301 aagaggtggg aacatgtgaa ggttcaaagg atagaagtgg agaataagcc gtatccattg
    15361 tatttattac tagataaaga ataccagcta atagaaccag taatgaaatt tattaaatac
    15421 ttagataata ctggtaagtc tcctaatacc attaaagcat actgctatca tttaaagttg
    15481 ctgtacgagt tcatggaaca gagaggtgtt attcttaatg atattaactt tgagttgtta
    15541 gcagacttcg taggttggtt gagatatcct tcagcatcaa atgtaattga tcttcagtca
    15601 aaaaaagcca taagagaaga aacgacagtg aatacaattt taaatgtagt tatgagtttt
    15661 cttgattatt taagtagatt aggagaattt aaatcaattg atgtatttaa acaagcaaag
    15721 ggaagaaatt tcaaaggatt tttacatcat gttaataagg gtagatacca aaagaatgtc
    15781 ttaaagttaa gggttaaaaa gaaacagata agaacattga gatcagagga agttaagcaa
    15841 attattgatg cttgtcatac gaaaagagat aaattaattt taatgcttat gtatgagggt
    15901 ggtttaagaa tcggtgaagt gttatcgctt aggcttgaag atattgtcac ttgggacaat
    15961 caaatccatt taacacctag agatgttaat gttaatgaag cttatattaa attaaggaag
    16021 gaaagaacaa tacatgtgag taaagaactt atgtcacttt atacagatta cttgatatat
    16081 gagtatagtg aggaattgga gcatgattat gtttttattt ccttaaaaga aggctatttt
    16141 gggaaaccac taaagtacca aagtgttctt gatctagtta gaagaatagt taaaaggact
    16201 ggaatagaat ttacatcaca tatgcttcgc cacactcacg caacgcagct aattagggaa
    16261 ggatgggatg ttgcgttcgt tcaaaagaga ttaggtcacg cacatgttca gacaacgtta
    16321 aatacctatg ttcatctttc agatcaggat atgaaaaatg agtttaataa atacctcgag
    16381 agaaaggagc ataagaaatg aatgcttcta gtaaaaggaa aattattagt cagagtgaga
    16441 ttagcaaaaa aatagctgta atgaatgaag aaatgcaggg gttttgggct aataatagtt
    16501 gggatataag aaaatgtcca catccttctg ccatagaatt aagtaagaat cctgctttaa
    16561 ggaatcgttg ggttcgtttt gaacgtgtta aaaatctgtg gttaagaaca gaattgaaat
    16621 atttttattt ttaccattta aacaatggaa tatggaatgc aaaaactgtc tggattagaa
    16681 aaggaacagt aattaataaa atgttagatt tcttagattt aaagtatcct agcattactt
    16741 caattactga agttcctatt gaaaaagcaa tgacggagta tagaacttat ttgacaaaac
    16801 ggggtgttag aattaccact actaattata agattactgc taatcaagaa aaaacacctg
    16861 taaaagctaa ttcctactat gttactaatc taaaacaatt tatggagttt tatgagaact
    16921 tttattttga tggagaggag tgggataaag acgtttggga tagacgtaac ttacctttgc
    16981 cagatgataa ggttaaccca acacaatatg aatatacaat taactttaaa gggtttcgga
    17041 atacatattt taaacaactt gtaaaaagat attgtaagtt gagattgaac atggatagct
    17101 tttcctatgt aagtgatatt gcccaaagac ttaaagagtt ctttaatttt ctggacatga
    17161 aatttaaaca agttcagaga gtacaccaat taacgagagt ggaaattgaa gcatatttaa
    17221 gtgaactaaa catgatggga ataaaaccta gtacaataac tgggaggatc tctatattgg
    17281 aaggactatt tagtaccctt cttaggctag aatgggatga tgttccttcc aaaatattaa
    17341 tttattctga ggactatccg aaaataccaa gagcaaaacc acgctttata gatgaattcg
    17401 tcctagagca attgaacagt catcttgata aattacccga atatatagct acgatgacta
    17461 tgattgttca agaatgtgga atgaggataa gtgaattgtg caccttgaaa aaaggctgtc
    17521 tattagagga caaagatgga gatttctttt taaagtatta tcaatggaaa atgaaaaagg
    17581 agcatatagt tccaatatct aaagaggtag ctttacttat taaagttcgg gaagataaag
    17641 tttcagagga atttccagat agtgaatacc tctttccaag aaaagatgga tcgccattaa
    17701 aacaagaaac atttagaggt gagttaaata aattagctta tgagcaaaat atagtggata
    17761 aatcaggtga gatttataga ttccatgccc atgcctttcg ccatacagta ggaacaagaa
    17821 tgattaacaa cgggatgccc cagcatattg tgcagaaatt tttggggcat gaaagcccag
    17881 aaatgacaag cagatacgct catatctttg atgaaactct aaaaaatgaa tttactaaat
    17941 ttcaggaaaa actggttacc aataatggag atgtgcttga tctagatgaa gataatgaag
    18001 tcgatgatgt agagcttcaa tggttcaaga aaaatataaa tgcacaagtg cttccaaatg
    18061 gttattgtag attgccagta gtagcaggtg gttgtccaca tgcgaatgca tgcttagatt
    18121 gcactcactt ctgtaccagt aagcaattct taccacagca cgaagaacag ttagagcgta
    18181 cagaagagtt attagccata gctaaggata aacaatggca aagacaagta gagactaata
    18241 gccgtgttaa agagcgttta gaacaaatca ttggaagttt gacggggtaa ttatcaatgg
    18301 ataaacaagt tagaaataca acagaaattg tacgtttggc gaagcagaaa tcaaaaaaga
    18361 caagggaaaa agtagacaaa gcgatttcta aattttcgat tgaaggtaaa gttattaatt
    18421 ttaattcaat agcaaaggaa gctaatgttt ctaaatcatg gctttataag gaacacgata
    18481 ttaggcaaag aatcgaatcc cttcgtgagc gtcaaataac agcaaatgta gtctcaaaac
    18541 ccaagaaaag ttctcgttcg gaggaaatcc ttattaaaac cttaaaaaga agagtaatgg
    18601 aattagaaaa agaaaataaa aaattacaga accaaattca aaaattatat ggagatctgt
    18661 ataataaaga ataattatta atctgtagac aaattgtgaa aggatgtact taaacgctaa
    18721 cggtcagctt tattgaacag tcatttaggt atatgtccaa tctagggtaa gtaaattgag
    18781 tatcaatata aactttatat gaacataatc agttacgagg tgaaatcatg agcaatttga
    18841 ttaacggaaa aataccaaat caagcgattc aaacattaaa aatcgtaaaa gatttatttg
    18901 gaagttcaat agttggagta tatctatttg gttcagcagt aaatggtggt ttacgcatta
    18961 acagcgatgt agatgttcta gtcgtcgtga atcatagttt acctcaatta actcgaaaaa
    19021 aactaacaga aagactaatg actatatcag gaaagattgg aaatacggat tctgttagac
    19081 cacttgaagt tacggttata aataggagtg aagttgtccc ttggcaatat cctccaaaaa
    19141 gagaatttat atacggtgag tggctcaggg gtgaatttga gaatggacaa attcaggaac
    19201 caagctatga tcctgatttg gctattgttt tagcacaagc aagaaagaat agtatttctc
    19261 tatttggtcc tgattcttca agtatacttg tctccgtacc tttgacagat attcgaagag
    19321 caattaagga ttctttgcca gaactaattg aggggataaa aggtgatgag cgtaatgtaa
    19381 ttttaaccct agctcgaatg tggcaaacag tgactactgg tgaaattacc tcgaaagatg
    19441 tcgctgcaga atgggctata cctcttttac ctaaagagca tgtaacttta ctggatatag
    19501 ctagaaaagg ctatcgggga gagtgtgatg ataagtggga aggactatat tcaaaggtga
    19561 aagcactcgt taagtatatg aaaaattcta tagaaacttc tctcaattag gctaatttta
    19621 ttgcaataac aggtgcttac ttttaaaact actgatttat tgataaatat tgaacaattt
    19681 ttgggaagaa taaagcgtcc tcttgtgaaa ttagagaacg ctttattact ttaatttagt
    19741 gaaacaattt gtaactattg aaaatagaaa gaaattgttc cttcgatagt ttattaatat
    19801 tagtgacatt tgcatgcttc aaagcctgtc ggaattggtt tttagtgaaa agaacacgat
    19861 attcacggtt tacccactta taaacaaaag atcgatactt tttgtagtcc ttctttgaaa
    19921 tcaatggttg atgtcgttca agaacaatca atacagagtc tacacttggc ttaggatgaa
    19981 aatatagtgg tggtactttt ttgagcattt ttatatccat ctccaccatt aatagtaaac
    20041 ccaaagctcg ttgcagattt tgcaatcttt tcgcaaatcc cttctcaacg ataagatagc
    20101 tatatttagc ctgactttca aaggtaattc ttttgacaat atccgtactg atgttataag
    20161 gaatattacc atatatctta tagtttatat gttttgggaa ggaaaatttt agaatatccg
    20221 tttgaatcac ttttatattc tcagaggggt ttaccgcttc tttagtcact tgacataagc
    20281 ctccatcaat ttctatagca gtaactgatc gactcatttt gactagctct ttggtaaaat
    20341 gtccttttcc tgatccgatt tctattacgt tgtcttgttt actgatattc gtgtgattca
    20401 atatttcttt tacatgcttt ttagaagtaa taaaattttg cgtgtcttta gggtttttct
    20461 ggttcattat aaccttctcc ttactggtta taatgaactg acattaagcg ttcattataa
    20521 ccaattaatt ttgatttggt tgataacgaa ctttattaat aacaaatata gaaaaagtac
    20581 ccatactttt atctcctttc catttttttc attatgaatg agataaagta atttctacta
    20641 ctgcgatact ggtgcacata attgaaacct ccttatgtaa ctgaatatga ttgtatcatc
    20701 ttttcatttt agaataaacc tttatatgta attttgcact tgacacttca gatggacgtt
    20761 ttcttcaaaa taatatgttt tgaaacttga taagagaagc cactgtcttt actaaaaagt
    20821 aaaggctgcg ataaaggaca gtgactttaa taaagttgca ctgttgtatt gtctttggat
    20881 aaaattgtct aaagggaagt tttatcccca acgaaaaaac atctaaagtg gtaagtaatg
    20941 aaagaatgtc gataaagtag gaatggggga tctttttgaa tacaatagaa tcacagtttg
    21001 ataaggttgc agaagattac gatttcgtga atgagctttt gaatgattat tcattctttg
    21061 tgtctaatat gtctccaaag aaaggcagag cattagatat cggatgtggc tcgggtttgt
    21121 tagtggagaa attagcaagt tattatgatg aagtggtagg gattgatatt tctaatcaaa
    21181 tgctcgatct tgccaaatct aaacgtcaac taacaaatac ggtctatctg aatatgaatg
    21241 cagaacaact taattttaat gagaagtttg attttattgt aagccgaaca acctttcatc
    21301 atttggatga tatagccagc gtaatacagc aaatgaagga actgttgaat gaggaaggaa
    21361 gaatagttat tcttgataat gtatctgaag tcgagacacc gcctacctat gtttataaat
    21421 taggggcaat ccaagagttt ttaccgcatt gtttcaaatt tggaataaaa aatgcgatta
    21481 gaatttataa tcacaacaca tctaaatcat ggcttgagca tttagcttca gataagtacc
    21541 tatctgaaca aaattattac gatttatatg aaaagttgtt gcctggatgc caattccata
    21601 aaatgggttg ggcaatgggg gttgtctgga caaaataact taaagctgtg gctttgaaaa
    21661 gttgaatttg tgattgtttt cagagctatc gggtgcttta gtataaataa caatgaataa
    21721 a
//
LOCUS       DADJYG010000034        21589 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.034, whole genome shotgun sequence.
ACCESSION   DADJYG010000034 DADJYG010000000
VERSION     DADJYG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.034"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(594..1985)
                     /locus_tag="JZ382_001034"
     CDS             complement(594..1985)
                     /locus_tag="JZ382_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY2427233.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="JZ382_001035"
     CDS             complement(2198..2584)
                     /locus_tag="JZ382_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAY2427234.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="JZ382_001036"
     CDS             complement(2640..3263)
                     /locus_tag="JZ382_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAY2427235.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="JZ382_001037"
     CDS             complement(3327..3995)
                     /locus_tag="JZ382_001037"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY2427236.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="JZ382_001038"
     CDS             complement(4007..4447)
                     /locus_tag="JZ382_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427237.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5178)
                     /locus_tag="JZ382_001039"
                     /pseudo
     CDS             complement(4506..5178)
                     /locus_tag="JZ382_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5363..5761)
                     /locus_tag="JZ382_001040"
     CDS             complement(5363..5761)
                     /locus_tag="JZ382_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY2427238.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5758..6531)
                     /locus_tag="JZ382_001041"
     CDS             complement(5758..6531)
                     /locus_tag="JZ382_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAY2427239.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6528..7211)
                     /locus_tag="JZ382_001042"
     CDS             complement(6528..7211)
                     /locus_tag="JZ382_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAY2427240.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7378..8265)
                     /locus_tag="JZ382_001043"
     CDS             complement(7378..8265)
                     /locus_tag="JZ382_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427241.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8262..9131)
                     /locus_tag="JZ382_001044"
     CDS             complement(8262..9131)
                     /locus_tag="JZ382_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427242.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9268..10554)
                     /locus_tag="JZ382_001045"
     CDS             complement(9268..10554)
                     /locus_tag="JZ382_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427243.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10827..11405
                     /locus_tag="JZ382_001046"
     CDS             10827..11405
                     /locus_tag="JZ382_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY2427244.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11405..12334
                     /locus_tag="JZ382_001047"
     CDS             11405..12334
                     /locus_tag="JZ382_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2427245.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12331..14067
                     /locus_tag="JZ382_001048"
     CDS             12331..14067
                     /locus_tag="JZ382_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2427246.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="JZ382_001049"
     CDS             complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="JZ382_001049"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAY2427247.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14963..15472)
                     /locus_tag="JZ382_001050"
     CDS             complement(14963..15472)
                     /locus_tag="JZ382_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAY2427248.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="JZ382_001051"
     CDS             complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="JZ382_001051"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY2427249.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16773..17864)
                     /locus_tag="JZ382_001052"
     CDS             complement(16773..17864)
                     /locus_tag="JZ382_001052"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAY2427250.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="JZ382_001053"
     CDS             complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="JZ382_001053"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY2427251.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="JZ382_001054"
     CDS             complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="JZ382_001054"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY2427252.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="JZ382_001055"
     CDS             complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="JZ382_001055"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAY2427253.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6249 a         4717 c         3770 g         6853 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttacgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaaatttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg tttttttact gatcactttt aaaaagcttt ttagaatctt
     4801 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4861 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4921 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4981 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     5041 atagatttct cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt
     5101 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     5161 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     5221 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     5281 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     5341 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     5401 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     5461 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5521 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5581 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5641 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5701 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5761 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5821 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5881 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5941 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     6001 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     6061 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     6121 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     6181 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     6241 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     6301 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     6361 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     6421 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6481 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6541 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6601 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6661 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6721 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6781 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6841 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6901 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6961 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     7021 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     7081 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     7141 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     7201 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     7261 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     7321 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     7381 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     7441 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7501 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7561 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7621 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7681 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7741 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7801 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7861 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7921 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7981 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     8041 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     8101 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     8161 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     8221 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     8281 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     8341 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     8401 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     8461 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8521 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8581 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8641 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8701 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8761 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8821 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8881 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8941 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     9001 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     9061 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     9121 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     9181 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     9241 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     9301 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     9361 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     9421 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9481 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9541 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9601 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9661 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9721 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9781 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9841 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9901 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9961 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
    10021 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
    10081 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
    10141 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
    10201 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
    10261 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
    10321 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
    10381 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
    10441 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10501 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10561 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10621 aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac
    10681 aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg
    10741 tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca
    10801 agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac
    10861 agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa
    10921 tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata
    10981 tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa
    11041 aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg
    11101 ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg
    11161 atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa
    11221 gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac
    11281 aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac
    11341 cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc
    11401 attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc
    11461 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
    11521 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
    11581 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
    11641 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
    11701 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
    11761 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
    11821 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
    11881 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
    11941 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
    12001 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
    12061 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
    12121 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
    12181 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
    12241 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
    12301 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
    12361 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
    12421 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
    12481 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
    12541 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
    12601 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
    12661 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
    12721 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
    12781 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
    12841 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
    12901 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
    12961 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
    13021 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
    13081 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
    13141 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
    13201 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
    13261 gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat
    13321 ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg
    13381 atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag
    13441 aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat
    13501 aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa
    13561 ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt
    13621 ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat
    13681 ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg
    13741 aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg
    13801 caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt
    13861 gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga
    13921 atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta
    13981 acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct
    14041 aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag
    14101 ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact
    14161 ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc
    14221 tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag
    14281 ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct
    14341 gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt
    14401 tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc
    14461 tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct
    14521 gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct
    14581 gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac
    14641 tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga
    14701 ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga
    14761 tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga
    14821 acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca
    14881 tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt
    14941 tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga
    15001 tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag
    15061 aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc
    15121 gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt
    15181 aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc
    15241 caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct gactccactt
    15301 ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt
    15361 cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac
    15421 cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct
    15481 cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct
    15541 tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata
    15601 ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg
    15661 atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc
    15721 accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg
    15781 attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga
    15841 atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc
    15901 gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca
    15961 tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc
    16021 ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt
    16081 atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg
    16141 atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca
    16201 acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc
    16261 gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg
    16321 attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt
    16381 tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta
    16441 aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg
    16501 tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc
    16561 tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa
    16621 tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg
    16681 atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt
    16741 atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta
    16801 ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc
    16861 agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc
    16921 gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata
    16981 aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt
    17041 tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct
    17101 gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg
    17161 ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga
    17221 aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga
    17281 ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca
    17341 tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt
    17401 ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat
    17461 agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg
    17521 acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca
    17581 tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag
    17641 gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa
    17701 tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga
    17761 ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa
    17821 gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt
    17881 ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga
    17941 atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc
    18001 aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg
    18061 ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat
    18121 gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt
    18181 attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga
    18241 gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat
    18301 actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc
    18361 taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt
    18421 tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg
    18481 tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc
    18541 tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag
    18601 aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg
    18661 ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg
    18721 tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac
    18781 agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga
    18841 accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt
    18901 catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc
    18961 ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg
    19021 ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct
    19081 ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt
    19141 ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta
    19201 gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc
    19261 cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta
    19321 ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg
    19381 tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt
    19441 ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt
    19501 cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga
    19561 tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat
    19621 caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat
    19681 tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt
    19741 gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca
    19801 ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat
    19861 cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa
    19921 ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag
    19981 caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg
    20041 atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat
    20101 gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg
    20161 aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt
    20221 ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac
    20281 gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca
    20341 aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa
    20401 tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg
    20461 ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca
    20521 ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac
    20581 agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta
    20641 ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa
    20701 ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata
    20761 tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc
    20821 cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt
    20881 cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg
    20941 gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc
    21001 atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac
    21061 tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac
    21121 tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc
    21181 tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac
    21241 attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact
    21301 tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac
    21361 atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt
    21421 ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt
    21481 tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat
    21541 tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgat
//
LOCUS       DADJYG010000035        21004 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.035, whole genome shotgun sequence.
ACCESSION   DADJYG010000035 DADJYG010000000
VERSION     DADJYG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.035"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            686..1375
                     /locus_tag="JZ382_001056"
     CDS             686..1375
                     /locus_tag="JZ382_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2427254.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1362..2570
                     /locus_tag="JZ382_001057"
     CDS             1362..2570
                     /locus_tag="JZ382_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2427255.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2864..4135
                     /gene="serS"
                     /locus_tag="JZ382_001058"
     CDS             2864..4135
                     /gene="serS"
                     /locus_tag="JZ382_001058"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY2427256.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4684..6168)
                     /gene="guaB"
                     /locus_tag="JZ382_001059"
     CDS             complement(4684..6168)
                     /gene="guaB"
                     /locus_tag="JZ382_001059"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY2427257.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6673..7368)
                     /locus_tag="JZ382_001060"
     CDS             complement(6673..7368)
                     /locus_tag="JZ382_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAY2427258.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7458..8558)
                     /gene="ychF"
                     /locus_tag="JZ382_001061"
     CDS             complement(7458..8558)
                     /gene="ychF"
                     /locus_tag="JZ382_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY2427259.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8567..8758)
                     /locus_tag="JZ382_001062"
     CDS             complement(8567..8758)
                     /locus_tag="JZ382_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAY2427260.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8779..9669)
                     /locus_tag="JZ382_001063"
     CDS             complement(8779..9669)
                     /locus_tag="JZ382_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY2427261.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9656..10423)
                     /locus_tag="JZ382_001064"
     CDS             complement(9656..10423)
                     /locus_tag="JZ382_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY2427262.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10435..11148)
                     /gene="rsmG"
                     /locus_tag="JZ382_001065"
     CDS             complement(10435..11148)
                     /gene="rsmG"
                     /locus_tag="JZ382_001065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY2427263.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11380..13281)
                     /gene="mnmG"
                     /locus_tag="JZ382_001066"
     CDS             complement(11380..13281)
                     /gene="mnmG"
                     /locus_tag="JZ382_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY2427264.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13298..14695)
                     /gene="mnmE"
                     /locus_tag="JZ382_001067"
     CDS             complement(13298..14695)
                     /gene="mnmE"
                     /locus_tag="JZ382_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY2427265.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            15039..15377
                     /locus_tag="JZ382_001068"
     CDS             15039..15377
                     /locus_tag="JZ382_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427266.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15545..16303)
                     /locus_tag="JZ382_001069"
     CDS             complement(15545..16303)
                     /locus_tag="JZ382_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAY2427267.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16321..17139)
                     /locus_tag="JZ382_001070"
     CDS             complement(16321..17139)
                     /locus_tag="JZ382_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY2427268.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17136..17492)
                     /gene="rnpA"
                     /locus_tag="JZ382_001071"
     CDS             complement(17136..17492)
                     /gene="rnpA"
                     /locus_tag="JZ382_001071"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY2427269.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17610..17744)
                     /gene="rpmH"
                     /locus_tag="JZ382_001072"
     CDS             complement(17610..17744)
                     /gene="rpmH"
                     /locus_tag="JZ382_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY2427270.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18267..19601
                     /gene="dnaA"
                     /locus_tag="JZ382_001073"
     CDS             18267..19601
                     /gene="dnaA"
                     /locus_tag="JZ382_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY2427271.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19799..20929
                     /gene="dnaN"
                     /locus_tag="JZ382_001074"
     CDS             19799..20929
                     /gene="dnaN"
                     /locus_tag="JZ382_001074"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY2427272.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         6005 a         4274 c         3848 g         6877 t
ORIGIN      
        1 cttgttaaaa atgtttgttg tcttgaattg aatcaagacg ccctacacat ttggtttgtc
       61 ttactttgtt cagttttcaa aggtctactt catcaggagt gtttcccgat tcgtttttgg
      121 ctgccgtagc aacttttata tcatatcaga gagacaagta attgtcaact cttttttaaa
      181 agttttttcg atttcgtttt tcgaaatctt ccagcaactc atttatcata acttatcgtt
      241 tgttatttgt caagaacttt tttgaagttt ttttcgatag aatcaagctt cgcaagtggt
      301 tcttttttaa cgactcagtt atcttatcat gtcatttcca acatgtcaac aacttatttt
      361 ttattattta ataagttatt ggtgttcttt tgaacaaata ataatatatc aacatcacat
      421 gtgaaagtca ataagaaaat gcttatttat caacatttct agcttacatg atcattggtt
      481 agttctggtc ctttttcgtt cgtctttata actccgagag acgtaaaatc cttttgtttt
      541 cagtttaatg ttcatgttct tgtcttattc acaccaacta gatgtctcac cttctaaaat
      601 tttccttaac aaagtgctgc ttttatctat ttctgctttt ttttgctaga ataatagtaa
      661 caatatgact ctggaggatt tatttatgaa aattttgatt gctgacgatg ataaagaaat
      721 cgttgaatta ttaagtatct atgttcacaa tgaggggtat gaagcagtaa aggcttatga
      781 tgggaaagaa gcattatcta aaatcagaac aatccctgat attgaattac ttattttaga
      841 tatcatgatg cctgagatgg atgggatgca agtagtgaaa gagctacgaa aagaatccca
      901 aataccaatc attatgctga cagcaaaaac tactgatatg gataagatca aaggactggt
      961 cgctggtgca gatgattatg tgaccaagcc atttaatccg ttagaagtaa tggctagagt
     1021 aaagtcttta ttacggcgta caaaaatgca gattgcccca gatcagcctg atgtattaga
     1081 agtaaatgca ttaatcatca ataaagactc tcatgaagtt aaaacaatcg atgggaaaga
     1141 aatccagtta acagctttgg aattcggtat cctctacttg cttgctagtc atcctaatcg
     1201 agtatttagc gcggatgaaa tttttgagcg tgtttggaaa caagaaagta ttgtctctgc
     1261 taaaactgtc atggttcacg ttagtcattt aagagataag attgaagaag caactggtgg
     1321 tgaaaaagtc attcaaaccg tttggggagt gggctataaa attgacgcaa gatagtaaac
     1381 gagcaactga aaaacgccgt cggatcaatt tgacttcaaa agaaatcagc gaattgttag
     1441 cggaaggaat catcacgatt atccttttgc ttctattaaa tgtttctatt ctggttgtta
     1501 tcagttcggt gatcaacagt tcaccttctt tgacgaatgc gatttgggat tcaaagaata
     1561 tttttgcaga acgattgaac actgatttgt tctggaacgg ccggaatttt attattccct
     1621 ttttctttct attagatatt ggtgttttgt attggcgctt gattcgaaga tatcgccaaa
     1681 tgcaattgcg gcatatcatc agcgaacttc actacatcgc aaacggaaat tatgatcatc
     1741 gtatcccttt tgaactgagt ggtgacttaa gccgagtggt aacaagtatc aatggattgg
     1801 tggatagtac cgtagcagcg attgaagacg aacgtaaaat cgaaaaatca aaagatgaat
     1861 tgatcacgaa tgtcagtcat gatatccgta ccccactcac gtccatcatt ggatacctag
     1921 gactgataga agatggccag tatcactcag aagaagatct tttaaagtat acgcatactg
     1981 cttatatcaa agcaaagcag atgaaatctt tagtggacga cttatttgaa tacactaagg
     2041 tacgccagcc tgctgtcccg gtgaattttt ctgcctttga catgatccag ttgatcgaac
     2101 agttagcagc tgattttgag ttggaagctt ctaaaaaaaa tatccaaata cttgttcaat
     2161 caaaagtaga ctcccttatc atggatggcg ataccgaaaa acttgtacgt gtcttcaata
     2221 atcttttgac gaatgcgttg aaatacggaa aaggagcaac gaagatcgta atcgaagtag
     2281 aacgtatcgg ttcagaagtt gttgcaacag tcaaaaataa cggagcaatg atcccacaac
     2341 aggcaatcga taatctattc gaccgttttt atcgtgtaga agaatctcgt tcacaagcaa
     2401 ctggaggaac tggacttggt ttagcaatcg ctcaaagcat tgtggctttg cacggcggat
     2461 acatttatgc aaaatcagat aagcaatgga cttctttcat catgcacctt ccaatcaaga
     2521 aaaatgaaaa attacctatt gatacacagg aagttattga cgaaagctga aatattcgct
     2581 attcttatta caattattag aataaatgaa aaacacagaa cccagtacac tttattcttt
     2641 ttcagagagc tgatggcagg tgtaaatcag caaaggataa tttggaagtt ccagttcgtt
     2701 tacgtgccct ttcgaaataa gaaaggtcgg ttcaacagcc gttatgtgtt caacaagtgc
     2761 agctgacgct gaacttgggt ggtaccgcga atttcagact gattcgtccc aagaataatg
     2821 ggatggatca gtcttttttt attttcaaaa aggaggaaat catatgttag atgtaaaaat
     2881 gatgcgacaa aatattgaag aagtacaaga aaaattgatg actcggggcg taaaaaaaga
     2941 aacattagct gaattcatgg aactagatga aaatcgtcgt aagctactag tcaaaagtga
     3001 agaattaaaa aaatatcgaa atgatgtttc tgctgagatc gcccaattga agcgtgcaaa
     3061 agaagatgct tctgctaaaa tcgctgaaat gaaagaagtc ggtggcaaca tcaaagcatt
     3121 ggatactgaa attgctgaag tggatgaaaa attgaaacaa attgcaacta ctttgccaaa
     3181 ccttccccat gattctgtgc cagttggaac agatgaagag gacaatgtag aaatccgtcg
     3241 ttggagcgaa ccacgtacat ttgcttttga accaaaacca cactgggaaa ttgcagaaaa
     3301 tcttgatatt ttagattttg aacgtggcgc aaaagtagcc ggtagccgtt ttgtctatta
     3361 caaaggatta ggtgctcgat tagaacgtgc tgtctacaac ttcatgcttg atcagcatat
     3421 ttacgaacac ggctatactg aaatgatcac tccttatatc gtcaacagta aagcgatgtt
     3481 tggtacggga caatttccaa aattcaaaga agatgttttc caattggaga atactgatct
     3541 aacattaatc ccaacagctg aagtaccatt aactaactat tataatgatg aaattttaga
     3601 tggtgcagat ttacctattt atttcactgc actaagccct gcttttcgtt ctgaagccgg
     3661 aagtgctggt cgggatacac gaggactgat tcgtcttcac caattcaata aagtagaaat
     3721 ggtcaaattt tcggatgtag agcattctta cgaagaattg gaaaaaatga cagctgatgc
     3781 agaagatatc ttgaaaaaat tgcatttgcc ttatcgtgtg atggccttat caactggaga
     3841 catgggcttt tctgcagcta aaacttatga cttagaagta tggatacctg cacaagatac
     3901 ttatcgtgaa atcagttctt gttctaactg tgaagatttc caagcacgcc gcgcaatgat
     3961 ccgttatcgc gataatgatg gaaaaatcca ctatactcat actctgaatg gttcaggact
     4021 tgcagttggc cgtacagtag ctgccatttt ggaaaactac caaaatgaag atggttctgt
     4081 cacagttcca gaagcgctcg ttccttatat ggggaatctg aaagttatta aataaagagg
     4141 ctgtgaaaaa gccgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac
     4201 atttttgcat aaattggact taataccgag aaactggctt ttgaacacca tttatcgaga
     4261 ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa atagtccctc
     4321 atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc gtttagtgga
     4381 gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa aacagctctt
     4441 catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact gtttattcaa
     4501 aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc
     4561 ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac ttccgccgtt
     4621 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatg
     4681 tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat catgagggtg
     4741 tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac gtaattgctg
     4801 tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca tttggaaaat
     4861 aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg gtactaattt
     4921 atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt ccatcgcgcc
     4981 taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt cgaattcacc
     5041 tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc cgccggcagc
     5101 taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa tcgctttgcc
     5161 atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta cacctacacc
     5221 ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta cgacatctac
     5281 acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg caatcaatgt
     5341 tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg cactatgtcc
     5401 atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat tcgcacgctc
     5461 aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat gttcatcttt
     5521 tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca atccgcttaa
     5581 acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca aaatcttttc
     5641 tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg tcatcacatc
     5701 agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa ttatccctac
     5761 caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat atttgctcat
     5821 caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat caatgattac
     5881 gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga tcgtcatgtt
     5941 tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct tgctgtcggt
     6001 aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt tagctaattg
     6061 tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca ataagacatc
     6121 atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt ttagtaccac
     6181 tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc aatgatctac
     6241 ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat ctgtgtatat
     6301 taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca atcaaaaaca
     6361 gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt ttgaacaccg
     6421 tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa attttgtgaa
     6481 aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca
     6541 ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta ctattattta
     6601 actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa aatagctgat
     6661 ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt attttttctt
     6721 caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta ctggatctaa
     6781 tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag
     6841 tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc ttcgatctgc
     6901 tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc agccaccgat
     6961 tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac gtgaaaacaa
     7021 tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat tatctaacca
     7081 catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga ttgcttccgt
     7141 tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt gttctgcaac
     7201 taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca aattcgctgc
     7261 ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta attttgtttc
     7321 taaactattg ttttctttta cgattgctcg taattgttct ggttccatta tctaatagtc
     7381 ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca tattctacag
     7441 ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc gtcttgtacg
     7501 acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg catgtttccg
     7561 taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg ttcgaaatct
     7621 gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt
     7681 acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata agcagcacgg
     7741 atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct
     7801 tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat cacttctgca
     7861 ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc tgcatctgct
     7921 acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa taaaaatagt
     7981 gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga ttttccttct
     8041 tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc tttatctttg
     8101 gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa gtctgctaac
     8161 actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc ttctacgtga
     8221 gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac ttggcggata
     8281 tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac gattccagca
     8341 atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag ttctgttaag
     8401 cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat cgtcgcaaag
     8461 ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt tgattttcct
     8521 acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac tctttctctg
     8581 catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc atcatgacga
     8641 tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg acttcccagc
     8701 gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag tcgtacataa
     8761 aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat caagaattcg
     8821 agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat ttttttcttg
     8881 gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc ggatataata
     8941 tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat tttgatttaa
     9001 ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg cacgattggc
     9061 taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt gtcccataga
     9121 taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca agcgcaaata
     9181 attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt gtgttagttt
     9241 caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca gatcttctcg
     9301 ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt ctctaacgat
     9361 agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct cccctgcaat
     9421 aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga cacctgattg
     9481 ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac ggggctgata
     9541 cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt ttacatccac
     9601 cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta atcctttact
     9661 tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct cctcgagaac
     9721 gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa cgaatatttc
     9781 ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct tctacgactt
     9841 catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg atctctaatt
     9901 ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt aatccttcca
     9961 atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta aatgaattga
    10021 ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac tgactgctta
    10081 cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc aattcgatct
    10141 ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt tcagtgatca
    10201 aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct cttgtcacat
    10261 caggttttct gatcccgaca ccactggtag catttccttg tgcatccata tccactagca
    10321 atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc gttgttttgc
    10381 cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct cctcctattt
    10441 gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg ttggtttctt
    10501 tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa ccgcctcttc
    10561 tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca attcctcttc
    10621 actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg gcaaacacaa
    10681 ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg
    10741 aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag catcgattcc
    10801 taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat caacgatcgt
    10861 tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg cccccgaacc
    10921 aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg taagtgaatc
    10981 gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt tcatcttttc
    11041 gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat gatcggtaag
    11101 ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa atattccttt
    11161 cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa aatctctaga
    11221 catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc cgatagactt
    11281 tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt tgagcaattt
    11341 ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt cactgacttt
    11401 cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag ggtttacgcc
    11461 actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt tttgttttgc
    11521 ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc
    11581 catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt catatttgat
    11641 ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg gaataaaccg
    11701 tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc
    11761 ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg ttggtttgat
    11821 tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct tcaaataatt
    11881 cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta atcgcaaatc
    11941 tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca aacgataagg
    12001 ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg
    12061 tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac cggcaatcag
    12121 cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag ctgtataaag
    12181 attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta ctacgtcata
    12241 ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa tagaatgaag
    12301 catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat agacttcttc
    12361 cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac
    12421 gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga tccctgtaaa
    12481 cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac ctgtataggt
    12541 cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga agctaaaatg
    12601 attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg tacttgattt
    12661 tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt cttttagatg
    12721 attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt tcaattcacc
    12781 gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt tggcccggta
    12841 gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga tcagttcttc
    12901 gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt
    12961 tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc cttttcctgt
    13021 gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt ctccgcctaa
    13081 cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag gattacatgg
    13141 catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc ccatacgtgc
    13201 agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc
    13261 atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa caaaattgac
    13321 tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct cctagatagt
    13381 cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa tcaatacctg
    13441 caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg cgggtattag
    13501 aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca gaaatagctg
    13561 attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact ggttcatttt
    13621 cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt ttatttagaa
    13681 gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct gctgtcagtg
    13741 gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct tttcggcttc
    13801 tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct gtatcgatta
    13861 gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga gtcgttcctg
    13921 caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc
    13981 cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc ttcccttgct
    14041 tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt tcaagcaata
    14101 acttcgtagt caattcttcg acatcatcat attctggata atcaatattt acttctactt
    14161 gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac agattgccgt
    14221 ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc aaatccatca
    14281 cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc
    14341 ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga ttgactacaa
    14401 cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc tttggtgctc
    14461 tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca acgatatgtc
    14521 cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat tgataaactt
    14581 tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt gcaccttctc
    14641 caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca gccatttttt
    14701 cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta tgcacaataa
    14761 acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat agattatcac
    14821 acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct gttctgcttc
    14881 cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt tattcgaaga
    14941 gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac taaatggtag
    15001 cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc agatctgctg
    15061 cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca gggaatatca
    15121 accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa gctgatttgt
    15181 ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa ttaaagacga
    15241 aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa
    15301 aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc caatttatcc
    15361 aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga agacagtttc
    15421 gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga taagaatgat
    15481 ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat
    15541 ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt tcctcacctt
    15601 ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt ttacgttcaa
    15661 atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt tcagccgtgc
    15721 gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct acattgacta
    15781 ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga tattgcaatg
    15841 cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt tctaaatgga
    15901 aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca tcaagctgtt
    15961 ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca cctagctcac
    16021 ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc tcagctgttg
    16081 gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact ttttcgctga
    16141 tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa cctttttttc
    16201 cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca gccagaccta
    16261 gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt acctccccta
    16321 ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc tttcgcggat
    16381 tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga tcagcagcgt
    16441 ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc tggctaaatt
    16501 gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca ttgatgcatt
    16561 cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga ttgctgctaa
    16621 aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga aatgcccttg
    16681 tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg gcatttgtac
    16741 taataaagga agacaaccag catatggatt gacgccattt tctgcatata aacgctgctg
    16801 ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc
    16861 ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa
    16921 cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc ctgcattacc
    16981 aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat cccaaattcc
    17041 tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga ccaaactaac
    17101 gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc ctctctgtcg
    17161 attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc ataagtcaac
    17221 cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat ttcttctttt
    17281 atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac cgcgttgccg
    17341 accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt ttccaaacgg
    17401 taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc ttgaaactct
    17461 cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc tatctctcac
    17521 ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct aaaaaagagt
    17581 ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct tcctttgcga
    17641 cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg gaatccgtga
    17701 actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc cacctccaaa
    17761 atgttcttat ctattttata aacacagaca tactttgata gtataacgac aaagtagtga
    17821 ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat tatttttttc
    17881 tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata ataaaaggct
    17941 ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg
    18001 tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc aagaggaaat
    18061 ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact ttttttgaca
    18121 actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat taaaaaattt
    18181 ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac tagttattca
    18241 ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg aatgaattaa
    18301 aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc gaaacagcga
    18361 accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag attcataaag
    18421 aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa
    18481 atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa gttatagaag
    18541 aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg aaccctaaat
    18601 atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca gcagcgctcg
    18661 ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga ggcgtgggac
    18721 ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt cagcctaatg
    18781 ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat tcgatccaaa
    18841 acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg ctagtggatg
    18901 acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat actttcaatg
    18961 ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca aacgagattc
    19021 cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt gatattacac
    19081 cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct gaacgtctag
    19141 agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac attcgtgaat
    19201 tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga
    19261 ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt
    19321 caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg aaagatctca
    19381 aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg tatttggcac
    19441 gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc aaggatcata
    19501 caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt gtcagtatgc
    19561 aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat tctatcaact
    19621 gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact ttttccacag
    19681 ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac atgccctatt
    19741 acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga gatacatcat
    19801 gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc aacgagctat
    19861 ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga cacaagaagg
    19921 tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt ctgttgaaaa
    19981 cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag cacgtttctt
    20041 tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt tagaaaataa
    20101 acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag atgcagataa
    20161 ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg tacacgtatt
    20221 gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc gtccaatctt
    20281 gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta ctgattctca
    20341 ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg atattgttat
    20401 tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag aaatcgtcga
    20461 aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt tctattcccg
    20521 tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt ttaatacaga
    20581 ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt tactttctca
    20641 tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg ttttatacgg
    20701 aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa gcagtggcga
    20761 tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg catttggaga
    20821 tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg aaccaacgga
    20881 agatggcgtt caatttattc agctgattac tcctgtacgt acaaattaac aatgattttc
    20941 aatgttcttg aaaatttaat tgaaaagaaa tagaaaaaat aaaattttaa acagaaggcc
    21001 ctaa
//
LOCUS       DADJYG010000036        20949 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.036, whole genome shotgun sequence.
ACCESSION   DADJYG010000036 DADJYG010000000
VERSION     DADJYG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.036"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            70..1338
                     /gene="rseP"
                     /locus_tag="JZ382_001075"
     CDS             70..1338
                     /gene="rseP"
                     /locus_tag="JZ382_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAY2427273.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1406..3115
                     /locus_tag="JZ382_001076"
     CDS             1406..3115
                     /locus_tag="JZ382_001076"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY2427274.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3327..7685
                     /locus_tag="JZ382_001077"
     CDS             3327..7685
                     /locus_tag="JZ382_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAY2427275.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7830..8303
                     /gene="rimP"
                     /locus_tag="JZ382_001078"
     CDS             7830..8303
                     /gene="rimP"
                     /locus_tag="JZ382_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY2427276.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8326..9501
                     /gene="nusA"
                     /locus_tag="JZ382_001079"
     CDS             8326..9501
                     /gene="nusA"
                     /locus_tag="JZ382_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY2427277.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9523..9816
                     /locus_tag="JZ382_001080"
     CDS             9523..9816
                     /locus_tag="JZ382_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAY2427278.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9813..10124
                     /locus_tag="JZ382_001081"
     CDS             9813..10124
                     /locus_tag="JZ382_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAY2427279.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10137..12443
                     /gene="infB"
                     /locus_tag="JZ382_001082"
     CDS             10137..12443
                     /gene="infB"
                     /locus_tag="JZ382_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY2427280.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12470..12817
                     /gene="rbfA"
                     /locus_tag="JZ382_001083"
     CDS             12470..12817
                     /gene="rbfA"
                     /locus_tag="JZ382_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY2427281.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(12974..13147)
                     /locus_tag="JZ382_001084"
     CDS             complement(12974..13147)
                     /locus_tag="JZ382_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427282.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13595..15957
                     /locus_tag="JZ382_001085"
                     /pseudo
     CDS             13595..15957
                     /locus_tag="JZ382_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16325..17248
                     /gene="truB"
                     /locus_tag="JZ382_001086"
     CDS             16325..17248
                     /gene="truB"
                     /locus_tag="JZ382_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY2427283.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17252..18193
                     /gene="ribF"
                     /locus_tag="JZ382_001087"
     CDS             17252..18193
                     /gene="ribF"
                     /locus_tag="JZ382_001087"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAY2427284.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18545..19330
                     /locus_tag="JZ382_001088"
     CDS             18545..19330
                     /locus_tag="JZ382_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2427285.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19343..20194
                     /locus_tag="JZ382_001089"
     CDS             19343..20194
                     /locus_tag="JZ382_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY2427286.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20318..20770)
                     /locus_tag="JZ382_001090"
     CDS             complement(20318..20770)
                     /locus_tag="JZ382_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2427287.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7176 a         3687 c         4563 g         5523 t
ORIGIN      
        1 tggtaaaatt atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag
       61 aaaaaagcta tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt
      121 catgagtttg gtcatttctt ctttgcgaaa cgatcaggaa tcctcgttcg cgaatttgct
      181 atcggtatgg gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt
      241 cgcctattac ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa
      301 atggcaccag gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata
      361 aatttaagta agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat
      421 ttagaagatg agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc
      481 taccctgttg atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg
      541 ccaaaagacg tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct
      601 ggacccatga ataactttat tttggcaatc ctgttattca tcattctggc atttatgcaa
      661 ggtggtgttc aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg
      721 gcagaagcag gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat
      781 tcatggaatg atctgacaac agtcattacg aaaaatccgg ataaaacatt agactttaag
      841 atcgaacgag aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat
      901 ggcgaaaaag ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag
      961 atcattggag ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga
     1021 tcactattta caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg
     1081 tcttcagaag cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca
     1141 atgaatctcg gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc
     1201 ttgaatattt ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg
     1261 acattagctg gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt
     1321 caacgattct ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt
     1381 ttgaggaaat aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg
     1441 cgaagttcca aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat
     1501 ccgccaagta tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa
     1561 actgaaacgc attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc
     1621 tgccatcttg ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa
     1681 cttgtatcgt ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga
     1741 aacttttact gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct
     1801 ttatcaaatc caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg
     1861 aagagagttt atcatgaaag atgcgtattc tttccatgca acggaagaaa gtttggacga
     1921 gacatataaa gattacgaaa aagcctacac cgaagtattc aagcgttgtg gcttagaatt
     1981 tcgctcgatt atcggagacg gtggcgcaat gggcgggaaa gactcaaaag aattcatggc
     2041 gatctcagaa atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa
     2101 cttggagatg gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt
     2161 ggagaaagta gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt
     2221 tgaacctcaa aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt
     2281 tttggtacgc ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga
     2341 ctttttagaa gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc
     2401 tgtcggacca gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga
     2461 tttagcgaat gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc
     2521 agaacgcgat ttcactgtac tttcttatga agatcttcgt tttgtacaag aaggagatcc
     2581 ttcaccagac aacaatggtg ttcttgcctt tactcgagga atcgaaatcg gacatatctt
     2641 caaaatcggc actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg
     2701 agaaaaattt gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat
     2761 cgtagaacaa cattcagatg aacaaggaat caactggcca aaaggaattg cgccatttga
     2821 tttgcatatc gtccaaatga atctaaaaga cgaataccaa acaaacctga cgaatgaact
     2881 agaagaagcg atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc
     2941 aggagtgaaa ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa
     3001 aaaagcagtg gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt
     3061 acgtaaagaa gaattggtga atactttacc aatcctcttg aaccaaggcg aataataagc
     3121 gttaaaacac agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc
     3181 ttttaaaatt ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa
     3241 aaaacgagta tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg
     3301 tttctcatca gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt
     3361 tggaacaaat ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa
     3421 tggatagtgt agtggtccat cgtaaatcgc gtttatggga atttacgctg catttttcca
     3481 aaattttgcc tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag
     3541 atattgcaca agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct
     3601 tgcaagatta ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc
     3661 aagtattgaa aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca
     3721 gtacaggagc tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg
     3781 tcagttacgg attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac
     3841 gagtattgaa actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag
     3901 cagctgaaag tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag
     3961 ctcttgaagg acccatccac atccagctag gacgaaatat tccagccgat gaaccaacga
     4021 caccaatgat cagtatcgtt gaagaagaac gaagagtcac acttgagggg tacgtattcg
     4081 acaaagaagt cagagaacta cgttcaaaac gcaagatctt gactttgaaa atcacagatt
     4141 atacttcatc atttatcgtc aaaaaattct cgaacggcga aaaagatgaa caagtatttg
     4201 atgcgatcag tgtcggtagc tggttaaagg tacgaggaag tattcaagaa gatacgtttg
     4261 tccgtgattt ggtcatgaat gcgcaagata tcatagaagt caaacataca cctcgcaaag
     4321 attatgctcc tgaaggggaa aaacgagtag agcttcatgt tcatagcaat atgagtacga
     4381 tggatgcaac aaacagcatc tctgatcttg tggcacaagc agggaaatgg ggacaccgag
     4441 ctatcgccat tacagaccac ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa
     4501 aaaaagcagg tgtgaaaatc ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc
     4561 caattgctta taatgatgca catgaagcat tgagtgaagc cacatatgtc gtatttgacg
     4621 tggaaacgac aggattgtct gcagtatatg acacgatcat cgagctagcc gcagtcaaaa
     4681 tgtataaagg aaatgtgatc gaaagctttg atgaatttat cgatccagga catcctttgt
     4741 cacgaacaac ggtagatctg actggtatca cggatggaat ggttcgagga tcaaagtcgg
     4801 aagaagaagt attgcggatg ttccttgaat tttcaaaaga tacaattttg gtagcccaca
     4861 acgcggcttt tgatatgggg ttcttgaata ctagctacgc aaggtatggt attcctgaag
     4921 cggccaatcc agtcattgat acgttggaat tagctagata tctgtatcct caattcaaac
     4981 gtttcggatt aggtgtgtta tctaaaaaat ttggtgtcag cttggaacag catcaccgag
     5041 caatctatga tgctgaagca actggtcact tagcatggat ctttgtgaaa gaggcaatgg
     5101 acaatcataa catgctttat catgatcagc taaatgagca tatcggagaa ggagattctt
     5161 acaaaagggc tcgacctttc catgtaacga ttttagcaaa aaatcaggca ggactgaaag
     5221 atttgttcaa actgatttca atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac
     5281 cacgctccca attgaaaaag atgcgtgaaa atctattgat cggctcagct tgtgataagg
     5341 gagaaatatt cgaagccatg atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat
     5401 tctacgatta tattgaagtt atgccaaaag cggtctatgc gcctttgatc gagcaagaat
     5461 tggtaaaaaa tgaacatgat ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa
     5521 gtttggataa aatcgttgtt gcaacaggta atgtacatta tctgaacgaa gaagatgcca
     5581 tttaccgaaa aattttgatc aattctatgg gcggagcgaa tccgttgaat cgtcatagtt
     5641 tgccggatgt ccatttccga acaaccgatg agatgctaac tgcatttcac tttttagggg
     5701 aagaaacagc gaaagagatc gtagtagaaa atacgaataa aatcgcagat atctgcgaag
     5761 aagtgattcc agtcaaagat gaactttata caccgaagat tccaggttca gaagatgaaa
     5821 tatcagaatt gagttacaca aaagcaaaac aaatgtacgg agatccgtta cctgagatca
     5881 ttcaaaaacg attaaaaaaa gagttgaatt ctattaatgg taatggcttc tcggttattt
     5941 atctgatcgc ccaaaaacta gtgcataaaa gtaatgaaga tggctatcta gtaggttcac
     6001 gtggctctgt tggctctagc tttgttgcga cgatgacagg aatcactgaa gttaatccgt
     6061 tagcccctca ttattattgt ccagagtgcc aatattccga gttttttgaa gatggtacgt
     6121 atggttctgg atttgacatg ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca
     6181 aagacggtca tgatattcca tttgagacat ttttaggatt ccatggagac aaagtgcccg
     6241 atattgattt gaacttctca ggtgattatc aagcagaagc ccataactat acgaaagtat
     6301 tgtttggcga agactatgtt taccgtgccg gaacgattgg tacagtcgcc gacaaaacag
     6361 catatggcta tgtaaaagga tacgaaaggg acaataatct gcagtttcga agtgcagaag
     6421 tcgacagatt agcaaaaggc gctactggag tcaaacggac aaccggccag catccagggg
     6481 ggatcatcgt tattcccgat tatatggatg tctatgattt tacgccgatc caatatccag
     6541 cagacgatca aaattcggaa tggaaaacga ctcattttga ttttcactcg attcacgata
     6601 atgtattgaa attagatatt ctagggcatg atgacccaac tgttattcgg atgcttcaag
     6661 atttatcagg gatcgatcca caaacgatcc ctacggatga cccggaagtg atgcggatat
     6721 ttgccggtcc tgaagtactt ggagtaagtc aggaacaaat ttattccaaa acagggacat
     6781 taggtattcc agaattcggc actcgttttg ttcgagggat gctagaagaa acgcatccta
     6841 caacttttgc agaactactg caaatttccg gactatctca tggtacagac gtatggttag
     6901 gaaatgcgga agaattgatt cgacgaggag atgcgacact ggcggaagta atcggctgtc
     6961 gtgatgatat tatggtttat ttgatccatg ctggattaga tagcgggatg gcatttaaaa
     7021 tcatggaaac tgttcgtaaa ggacaatgga acaagatccc tgatgaattg cgagaaacct
     7081 atctatcggc gatgaaagaa aataatgtgc ccgactggta tatcgattct tgttcaaaaa
     7141 tcaaatatat gtttccgaaa gcccatgctg cagcttacgt attgatggct cttcgtgtcg
     7201 cttatttcaa ggtttatttc cctattttgt attactgtgc atacttttct gtacgtgcag
     7261 acgactttga tctagtctcc atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag
     7321 aaatcacgga caaaggatta gatgcttccg taaaagaaaa gaatcaactg acagtgctgg
     7381 agttagccaa tgaaatgctg gaacgaggat tcaaatttgg aatgattgat ttgtataaat
     7441 cggatgccgt taactttgtt attgaaggtg atacgttgat cgcaccattt agagctgtcc
     7501 caagtttggg aaccaacgtg gcaaaacaaa tcgttgaagc aagaaaagac ggtccatttt
     7561 tatccaaaga agatcttgcc acccgcggta aagtttcaaa aacattgatc gaatatatga
     7621 acgacaatgg cgtattgaaa gatctaccag atgaaaatca attatcgtta ttcgatatgc
     7681 tataagcatc tgttttcctg tctgcttgta attttaagca gagtatggta tagttcttat
     7741 aggtaatgat actaactgtg agtgagcgga tttttcgctc actcttttta atggaattac
     7801 gcacttaatt ttttaaggag gcgaaacttt tgagtagcgt cgttgaaaca gtcacagaaa
     7861 tggtgacacc gattttagat gaacaaaatt ttgaacttgt cgaagtcgaa tttgtaaaag
     7921 aaggaaaaag ctggttctta cgagtattta ttgacaaaga aggcggtatc gatatcgaag
     7981 aatgcgcctt tgtcagtgag aaactgagtg aaaaacttga tgcagcagat ccagatccga
     8041 ttcctcaagc ttatttcctg gaggtatcct ccccgggagc agagcggcca ttgaaaaaag
     8101 aaagcgacta tgaaaaggca cttggtgatt atgtccatgt ctccttatac caaccagtag
     8161 atggcgaaaa acaatatgaa ggatttcttc aatcatttga tgctgaacag cttacattga
     8221 aaatacgcat aaaaacacga gaaaaagaat tgacttttga ccgaaaaaat attgcaaaag
     8281 ctcgcttagc tatccaattt taatcacgga gaggaatcag aaaaaatgag taaagaaatg
     8341 ttaaatgcgt tagacgcttt agaagctgaa aaagggatct caaaagaaat cgtgatcgat
     8401 gctttagaag cagcattggt ttctgcatac aaacgtcact atggccaagc acaaaacgtc
     8461 gaagtggaat ttgatcagaa aaaaggaaag atccatgtct atgcggtaaa agaagtaacg
     8521 gaagaagtga tggattcaca gttggaagtc tcattgaaag atgcactttt aatcaatcca
     8581 gcatatgaga tcggtgatac gatccgtttt gaagtaacac caaaagattt tgggcggatc
     8641 gctgcgcaaa cggctaaaca agttatttta cagcgtgtac gtgaagcaga aagaacgatt
     8701 atttataacg aattcagtgc ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag
     8761 gataaacgtt atatttatgt caatcttggt aaaattgagg cagttctttc taaacaagat
     8821 caaatgccaa atgaatttta tcaaccacat gatcgaatca aagtttatgt ttcccgagta
     8881 gaaaatacgt ctaaaggtcc gcaagtattt gttagccgca gccatccaga cctattgcgc
     8941 cgcttatttg agcaagaagt accagaagtc tatgatggaa tcgttgaaat tgtcagcatc
     9001 gcccgtgaag ctggcgatcg ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat
     9061 gcagtcggta catgtgtcgg gccaaaggga caacgagttc aagcaatcgt caatgaatta
     9121 aaaggcgaaa acatggatat cgtcgaatgg aatgaagatc cagctgtatt cattgcaaat
     9181 gcattgaatc ctgcacaagt catggatgtg atcttcgatg agacaaatcc aaaggcctgc
     9241 acagttgtcg ttccagacta ccaattatcg ctagcaatcg gtaaacgcgg acaaaacgca
     9301 cgtttagctg ctaaattgac taaccataaa atcgatatca aatcagaatc tgacatgacg
     9361 gaattttatg aaaatcaaga acaacaaaaa gaaacagaag aactgcatga tgaagcaatc
     9421 gttcagtcag atttaacaga cgatgaatat gaaacaattg cattcaacaa tgaaacggtc
     9481 gaagaaaaac cagaagctta actttagtaa ggaggcaaag agatgaaaca aaggaaaatt
     9541 cctttacgca aatccgtggt ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc
     9601 acccgttcaa aagaagggga agtatcgatc gatccaactg gaaaaatgcc tggacgagga
     9661 gcgtatgtct cacttgagcc ggaagaagta caacaagctt gggataaaaa aattctcgat
     9721 cgagtactcg aaacaacatt aacagatgag ttttatcaag aacttcttga ctatgtcact
     9781 caccaaaaag cacgtaaaga gttatttgga aaatgaatac agttaatcgg caaaaagcca
     9841 tgaatctcat cggtctagcg atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca
     9901 ttgctgaaat ccgcaaacaa aaagcaaaat tggtgtttgt ggcaaccgat gcaagcgaga
     9961 atacaagaaa aaagataaaa gataagagtt catattatga agttccttgc tttgagctat
    10021 tttctgaagc agaaatcacg cagatgatcg gaaaacctcg caaggtattc ggaataatgg
    10081 atgccggatt tgcgaagaag accaaggagc taattgaagg ttaggaaggt gattgcatgg
    10141 gcaacaaaag aatctatgaa ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg
    10201 aaaaagcaca acaacttggc atcgatgtga aaaaccatat gggtgcgatt actagcaatg
    10261 atgagaagaa attgcaacaa gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac
    10321 aagcaagcag aaaaccagcc aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga
    10381 acaatcaaca aaaaaataaa aataaccgca gttatcaaga tcgcggtcaa gggagcggtc
    10441 aagtgaatca aggaaaaaaa ccaacaacaa accaaaataa tacgggaaat accagcagcc
    10501 aaaatcgtca aggaacgagc caaggcggca atcaaaaccg tactaacaat aacaacaaac
    10561 caaacaataa caatcgcggc aaatttaata ataataaccg taatcgttat aataaaaaag
    10621 gtaaaaaagg caaacagcaa caatcaacta aaccagctgt accaccacgc aaattccgtg
    10681 aattgccaga tgtattagag tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa
    10741 ttcatcgtga gccagcagaa atcattaaaa aattatttat gcttggtgtc atggtcaatc
    10801 aaaaccaagc actggataaa gatacgattg aactgttagc gacggactat ggcatggaac
    10861 cacaagaaaa agtccaagtt gacattgctg atatcgataa attcttcgag gcagaagaag
    10921 tcgatccaga taaattagtc tcacgtccgc cagtcgtaac gattatggga cacgtagacc
    10981 atgggaaaac aactttattg gatacattac gccattcacg tgtaacaagc ggcgaagccg
    11041 gcggaatcac acagcatatc ggtgcctacc agatcgatat tgatggtaaa ccaatcactt
    11101 tcttggatac accaggacat gcggccttta caagtatgcg tgcacgtgga gcaagtatca
    11161 cagatatcac gatcttagtt gttgctgcag atgatggcgt catgccacaa acagttgaag
    11221 caatcaacca tgccaaagct gcaggcgttc caatcatcgt cgctgtaaat aaaattgata
    11281 aaccaggtgc aaaccctcaa catgttatgc aagaattaag tgagtatgag ttaattcctg
    11341 aagcatgggg tggagaaacg atctttgttg agatctcagc gaaattcggc caaaacatcg
    11401 aagaattgct ggaaatgatc ctgttagtcg cagaagtaga agatttaaaa gcagatccaa
    11461 cacaacgcgc aatcggaaca gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt
    11521 caaccttact tgtccaagaa ggaacacttc atgttggtga cccaatcgtt gtcggtaata
    11581 cttacggacg tgtccgtgta atgacaaacg atcttggccg tcgcgataaa gaagcaggac
    11641 ctgcaacgcc agtagaaatc actggcttga atgatgtacc tcaagcaggt gaccgtttcg
    11701 tagtcttcga agacgaaaag acagcacgtg cagcgggtga agaacgcgcg aaacgtgcat
    11761 tattagaaaa ccgtgctgcc acaagccgtg tgactttgga taacttattt gaaagcctga
    11821 aagaaggcga attgaaagaa gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag
    11881 aagcactggc tgcatcattg aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc
    11941 attcagctgt cggagcaatc aatgaaagtg atgtaacctt agcagcagcc agcaacgcta
    12001 ttatcatcgg tttcaacgtt cgtcctacac cgcaggcaaa agtgcaagct gatactgaag
    12061 aagtcgatat tcgtcttcac cgtatcatct ataaagccat cgaagaaatc gaaactgcga
    12121 tgaaagggat gctagaccct gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg
    12181 aaaccttcaa agtttctaaa gttggtacga ttgcaggtgc ctatgtaaca gaaggatata
    12241 ttcgtagaga tagcggtgtc cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac
    12301 ttgcaagtct gaaacgattc aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg
    12361 gtgcaatgat tgaaaaattc aatgacatca aagtagatga cgtaattgaa ggatttatca
    12421 tggaagaagt caaaaatgac taaaaactga taaaaggagg ccaatcatta tggctaacta
    12481 ccgtgaccgc cgagtcggtc aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa
    12541 ggtccgtgac cctcgtgtgg agaacgtaac gatcacagat gtgaaagtta cgggggactt
    12601 acagcaagca acgatctatt acagcatttt atctgactta gcatcagata agaaaacagc
    12661 acaagatgga ttgaataaag catcaggatt gattcgtcga gagttagggc acaatctaag
    12721 tatttataag acacctgaat tgttctttga acttgatgaa tcagttgtct atggcaacca
    12781 tatcgatgaa ttgcttcgcg acttgaacaa agactaatca aaaactcccg caaatcctgt
    12841 gaaatcaggg gttgcgggat ttttttgttt tcattaagac gtattaccta agagttccat
    12901 gactttagtc ttgcggcgtg tcagctaacc aaaaaagaga aaaagaaaag taaaaaattc
    12961 gactttcttt tttctagtag tatataaatt tttgcatata gataaagatc aagataccga
    13021 taacggaagc tagattgtaa tctattttag tgacagtcag gtatagccaa gtaactagaa
    13081 gatcagtgga acctgcacca taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa
    13141 aaatcacgtt gaagttattg atatttccga ttacgccgct tcgatttggt cacgatccaa
    13201 attttggata atccctgtag aaattagcat agtagctggg ataccaattc acatattgac
    13261 aaagataaca gagactttta cataaaacgg atcaatcagg aaaggaattg ctcgatccag
    13321 ccccagtttt ccagcaatgt gttaactggc ccagcgtcat tcagcaaatt atgcatgatg
    13381 aggagcgaaa tgaactgcgg aacagccatt gtgataacga caagtttttt ggatcaatgt
    13441 aaaaaacgtc agcgaaagga aaaatttcgt ggttttttat acctgttcat ttggctagat
    13501 ataaaatcca aacaaatgaa cagaagtgct tattaggttt caattgggag tatctgcgct
    13561 agaataagag taataatgaa aaggagtgtt atgtatggat tattctagta aagaatactt
    13621 tgataaaatg acggcttggt ggcgtgctgc taattatctt tctgttgggc aactttatct
    13681 taaagataac cccttactga gacgtacact gaaacccgaa gatgtcaaaa aacatccaat
    13741 cggtcattgg ggaacgattc ctggccaaaa ttttatttat gtccatttga accgagtcat
    13801 caataaatat gatttgaaca tgttttatat tgaagggcca ggacacggcg gacaagtcat
    13861 ggtatcgaat gcttatttag atggaagtta cacagagatc tatccagaag taacggaaga
    13921 tgagacagga atgcaaaaat tattcaaacg attttcattt cctggaggca ttgcttctca
    13981 tgcagcacca gaaacaccag gttctatcca tgaaggcgga gagcttggat actctctttc
    14041 acatgcagtc ggagctgtat tagacaatcc tgaagtaatc tctgctgtcg tgattggtga
    14101 tggtgaagca gaaaccggtc ctttagctgg aagctggttc tcaaatgtct ttatcaatcc
    14161 agtgatagat ggagctgttt taccgatcct tcatttaaat ggtgcaaaaa tagccaaccc
    14221 aacgatcttg gcacgtaaat cagatggaga attagcaaat tactttaacg gacttggctg
    14281 ggaacctttc tttattgaag gaaatgattc agaaaaatta aatcctgtta tggctgaaaa
    14341 aatggatcaa gcaatcgaga aaattaaatc catccaaaaa gaagctcgcc tgaagacagc
    14401 aacagatgtc gtgatgccaa aatggccagt attgatcgta cgcacaccaa aaggatggac
    14461 cggtccaaaa gaatgggacg gggaaccaat tgaaggaacc ttccgagcac accaagtacc
    14521 gattcctgta gatcaagaac atatggatca tgcagatgct ttactccgtt ggctgaaatc
    14581 ctatgaacca gaaaaattat ttgatgcaca aggacgtatt ttagaagaaa tcagagaaat
    14641 cgcgcctact ggtgaccaaa gaatggcaaa aaatcctatc accaatggtg gaatcgatcc
    14701 aagaccgctt attatgccag attggaaaaa atatactttg caatttgaaa aaccaggctc
    14761 cgttaaagca gaagatatga ccgaacttgg taaattcgtt cgcgagatca ttgagaaaaa
    14821 tccagaaaat ttccgtatat ttggaccaga tgaaacaaaa tcaaaccgat tgaatcaagt
    14881 tttcaaaacg acaaatcgcc aatggatgga aaaaatcgaa ccagaaaatg atgaatggct
    14941 ttcgccatct ggtcgcgtca tcgacagtca attatcggaa caccaagatg aagggttctt
    15001 agaagggtac gttctgactg gacggcatgg ttttttgcaa gttacgaatc attcttgcga
    15061 gtggttgact ctatgttgac tcagcacttc aaatggatgc ggaaatcaca tgacctttct
    15121 tggcgaaatg attatccttc gctaaatctc attgcaagct caacagtctt ccagcaagat
    15181 cacaatggat atagccacca agatccagga atattgacac atttggcaga aaagaaggcg
    15241 gagttcatcc gtgaatatct gcctgcggat gcaaatacat tgctcgcagt catggataaa
    15301 gcattccgct cgtcagaaaa aatcaatttg atcatctctt ccaaacatcc gcgtgctcaa
    15361 ttttactcag cagaagaagc agccgtctta gtgaatgaag gcttgaaaat cattgattgg
    15421 gcttcgacag caaaagagga ggaacctgaa ttggtcattg ctgctgcagg aacggaatct
    15481 aacttagaag cattagcagc tgtaactttg ctgctggaag aattcccaaa actaaaaatt
    15541 cggttcatca atgtcgtaga tttattgaaa ttacgtcatc caagtcaaga tccgcgtggg
    15601 ttgagcgatg aagaatttga tcaatatttt acaaaagaca aaccgatctt atttgctttc
    15661 cacggttatg aaacattggt ccgcacgatt ttctttgatc gtcataacca tcatttaatg
    15721 attcatggct acaaagaaaa tggagatatc acgacaccat ttgacatgcg tgtagtaaat
    15781 gaacttgatc gttatcattt ggcaaaagac gcagctttaa aaatcaaagg aagtcaagca
    15841 gaagattttg cgaaaaaaat ggatcagaaa ttacaagaac atcaaaacta tattcgtgaa
    15901 aatggtattg atctgccaga agtattggat tggaaatgga aaaatcttga ccaataaaat
    15961 gataaaatcg atcccgcaaa atagaatttt ttacattgat taagaaactg aaaaaataaa
    16021 taaaaaaaac ggaaaaaaga ctggaagaat ggccagtctt ttttttcgtt tttgggaagt
    16081 gaaaaccaaa ctatttacct gtaagccacc cataaatcca atcaaatggt gcaaagaaaa
    16141 aatcttccat tctattccct cctttaaaaa agcataacat gatttctatt tacgtgagca
    16201 tgaattttgt tacgttttta ttgccgaaaa ataaaaaccg ttcattatga tgtcaaattc
    16261 gtcagtaatt gatgcaatgt gttataataa ttcagttaaa tcaaaaatga aatggagaaa
    16321 agacatggac gggttattac ctttatggaa agaacgcgga atgacaagtc atgattgcgt
    16381 attcaaatta agaaaaatct tacatacgaa aaaaatcgga catgggggta cgcttgaccc
    16441 agacgtagac ggtgtgttgc ccatttgtat tggaaaagcc acaaaagtta tcgaatattt
    16501 gacagattct gggaaaatct atgtaggcga gataactcta ggtttcagta caacgacgga
    16561 agatcgatcc ggagaacggg tagaaacaaa accagtagat atgtctttat ctgaaaaaaa
    16621 aatagatgaa gtgatggctt catttattgg tgagatcacc cagataccac caatgtactc
    16681 agcggtaaaa gtcaacggta agcggttata tgaatatgcc cgcaataatg aaacagtgga
    16741 gcgtcctgtt agaaaggctc agatttaccg ttttatccgg acaagtgaat tgacaagaga
    16801 cgaagtgaca ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag gaacctatgt
    16861 ccgcacatta gctgtagata caggcaagaa acttggttac cctgcgcaca tgtctgatct
    16921 gacccgaaca gcaagtgccg gtatgtccca gaaggatgct ctgacattag aagaagtagc
    16981 tcaagctgtg aaaaatggga caatcgatga ttgtttgctg ccaatagaaa aaggagtgga
    17041 acggttcaaa cgagtagata ttactgatga agtatggcag aaagtaaaaa acggtatgag
    17101 acttgattat actgtatttg cgctcccaga aatgccgtca gaagaaattg ctttgtttta
    17161 taaaggaaaa gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca aattaaaacc
    17221 tagtaaagta ttgagaaatg aggaataacg aatgaatatc atcaagatca gacatcctta
    17281 ccaatcagaa caaattccag aagacgatgt cgttttagtg ctgggatttt tcgacggcgt
    17341 gcatatcgga caccagaaag tcataaagac aggtatggaa attgcacaaa aagaaggatt
    17401 gaaattagca ttgatgactt tcaatcagca tccatctatt gtctttaaaa agatcgatcc
    17461 tagcagcgtg aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg ccgctttggg
    17521 tatagattat ttatatgaag ttgattttac ttcttctttt gctcatttag caccgcagga
    17581 atttgttgat caatacatcg tagggttaca tgcaaaatat gctgtttccg gttttgatta
    17641 tacgtatgga ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg ccaaaggaag
    17701 attcaaaatc gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca gctctacccg
    17761 tattcgtgga ttactagaca gcggacaagt aaaagaagca gcaaaactat taggttgcac
    17821 atatgaggta gaaggggtag ttgtccatgg cgatgcacga ggcagactac taggattccc
    17881 tacagcaaat gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg tttatgtgtc
    17941 ggaaataaaa attggggaaa catggtatcc agccatgggt tcaattggac ataatgatac
    18001 attcggtgaa ggacgggagc tgactgtaga gttgtatatt ttggacttta atcaagatat
    18061 ctatggtgaa catgtcgcgg tacgctggaa tcaattttta agagatcaag tgaaattttc
    18121 tagtgtagag gaattgattg aacaactcaa ggaggatgaa caagcaacag ctgaatattt
    18181 ttctcagcaa taaaacatta tgcataaaat ctcttgttta tgtataacta gcaattacaa
    18241 taaaatgggt gataaataag cccaggaaag gggctgtgac aaaagtcttg tcacagcctt
    18301 tttttccaaa taaatggttt ttcagaagta gcttcttcgg aaataagccg aaatttcttg
    18361 aagctgacca cttctgtcac aacctctttc tttatagaaa taaaaggaca ctagcaattc
    18421 atgaaaacga ttgctagagc gtatcatttg ctttagacta tatatcttgg tgtacactca
    18481 ctttaaggcg gaaataaaag ttatccgtaa taacaggttt aggtatgaaa aaggagtggg
    18541 tactatgtac aaggaattag aaaacaaagt cgcagtagta actggtggct caaaggggat
    18601 tggaacagcg attgctcaac gattcgggaa agaaaaaatg aatgtagttg tcaactataa
    18661 ctcggataaa gaaggagcag aaaaagcagt caaagcaatc gaagaggcag gcggaaaagc
    18721 agtctctgta caagcagatg tcagttctga agaaggaata cagcgtttac ttgatacggc
    18781 tttagaaaat tttgggacac tggacgtttg ggttaataat gctgggatgg aaaatcagca
    18841 tccaacccat gaacttagcc ttgaagattg ggaaagagtg ttaaaagtca atttgactgg
    18901 cgtttttatc ggaacgaaag cggctttaaa ttactttaca gagaataata aaaaaggaaa
    18961 tatcatcaat atgtcttctg ttcatgaaca gatcccatgg ccaacgtttg ctcattacgc
    19021 tgcttcaaaa ggcggggtaa aattatttac cgaaacggta gcaatggagt atgcaccgaa
    19081 gggaatcaga atcaatagta taggccctgg tgctatccgt acacctatta atgcggcaaa
    19141 atttgatgat cctgaacaaa aagaactctt ggaaagtatg atcccactag gacgcgtagg
    19201 tgaaccaaaa gaaatagcgg cgacagctgc atggttggct tcagacgaat ccagttatgt
    19261 tacaggtatt acactatttg cagatggtgg gatgacttta tatccttctt tccaaggtgg
    19321 aaaaggataa gggtgaataa aaatgggttt attgattgca ttgatcccgg cagttggctg
    19381 ggggatccag ccactgattt tgaaaaagat tggtggcaga ccaacaaatg agatattagg
    19441 aactggtatt ggagcggtgc ttgtcggctt actcgttcaa ttattcgctt ctccagctgg
    19501 tatttcagtg actacatttt tgattagttt actttctggt gcattttggg ttattggaca
    19561 aacaggacag tatacatctt tcaacttgat tggtgtttct aaaacgatgc caatttcgac
    19621 tgcattacag ttagtcggaa cctcgttgat tggtgtcttc gcttttggag aatggtctgg
    19681 aagtatgggg aaaatcattg gagcagcagc aatcattctt cttgtatttg gttctgcttt
    19741 aacggcaatc agtgatgatc ctggaaaaaa aggcggtatc acaaaaggaa ttagtctatt
    19801 agcatcaaca agtattggtt attgggtata cagtgcattg cctaaactag ttgatgcaag
    19861 cggactttcc attttctttc cgcaaatgat cggtgtcttt ttaggggcag tgatttatgt
    19921 gattttccgt aaaccaaccg cattttctga agacaagagt tggaaagcaa cgatcgttgg
    19981 agccatcttt agtgtgtcag cattggctta tattttttca gcaaatgaaa atggggttgc
    20041 tactgcatat atcattacac agctaaacgt agttatctct acattaggcg gtatgtacat
    20101 cctgcatgaa aataagacac caaaagaaaa aaaattaacg attatcggat tattgctgat
    20161 cgtagtagga agcatggtca cggtctttat ttgaggataa agatggtaaa atttcattta
    20221 ttaaaaaaat atttgtttga accaaacagt aagtacttat aaaaaaatag cgctagaatt
    20281 tccaacatct ctggaatttc tagcgctttt taattgttta agatacaagg gaaatatctg
    20341 tctggatcaa taatttttca ttgatccatt gactaaagtt tacgacataa ttgaagttac
    20401 caccgattag tatctccgca tgattgtctt tataaagatc gtattgtaaa agacggtctt
    20461 ttttatttgc gtaaacatcg ttatggtact ggactaattg ttctcggaat tgattgattg
    20521 ctgtaaaatc acgatattga aggttgaaac gcatcgtgat tggtgcattt agcatgaatc
    20581 gctgcatttc ttttgctgct tcctctgctt gatctccgta taggaactca aaaagattac
    20641 gttcagaaaa tttccattgc ccgccaacct ttgttccttt tagttttcct tcatgaagat
    20701 aatttctgat agaacgtgta gtaacaccta attgttcggc taattcattt actgagtaaa
    20761 aatctttcaa tgttcaccct cctatgtgaa tggttctact gacttagttg atatttgctt
    20821 aatcacgaaa tgtagaaaga acgattttaa ttaaggtcat tgtaaaaatc aagtcagaaa
    20881 attctaactt ttatttctct aaagaaattc gttctctcct aaagtgatat tatatttaaa
    20941 tatacctga
//
LOCUS       DADJYG010000037        20792 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.037, whole genome shotgun sequence.
ACCESSION   DADJYG010000037 DADJYG010000000
VERSION     DADJYG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.037"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..856
                     /locus_tag="JZ382_001091"
     CDS             11..856
                     /locus_tag="JZ382_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427288.1"
                     /translation="MDKMKLIGIDLDGTLLNSDQSISQKNARTMQHLPKDCFPFICSG
                     REVEDINNILKKSGLMVPAVGLNGSVGFDGEKKIFESFFDKKDVKKIDSVISKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSVKELNYELEYEKSICSIPYNNIEEIIEKDD
                     ISIYKFFIFIPNRQLKANIFNRLKKMLNVTVTESAPVNIEIVPQNVSKGTVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESANISFAMGNSHQEIMKKADYITVSNDKDGVSQAFE
                     RILTI"
     gene            1054..2274
                     /locus_tag="JZ382_001092"
     CDS             1054..2274
                     /locus_tag="JZ382_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAY2427289.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGTSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQTSTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRQARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRIRDLFIWRISLMEDTF
                     YTKIPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAAKILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2271..4991
                     /locus_tag="JZ382_001093"
     CDS             2271..4991
                     /locus_tag="JZ382_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2427290.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPIYGEFAVERYKQQNRGKSKVWLGNQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHLKQEQEAYFGKLIQQEQEYES
                     QQQQLLEQLNEANKQFALARQQELNFSLYEEWQSLKKNIATGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTTEQLSEEWQRL
                     QIETRETNSELKILEQRWQWQKNPLPEEFLYEEEWKNLLAEERRIADLESQISVRLQI
                     AKEQQKMIEAEVNELEETYPALLQNQSEIAPTPSSKPYMLIAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKQQPKVKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     IEEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFVRKHKREQEIQ
                     EKLQQIDQKFEFVKEWLPIQDKTISEKMAMLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWVQEYSEQLSQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLRPLFPE
                     ELTKEKLSVRLRQLKEKQGQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGAFEIAGTFLGDLSTEMSERQLPQLLKAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTDFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQTVKQLEGANNEKNS"
     gene            4975..5919
                     /locus_tag="JZ382_001094"
     CDS             4975..5919
                     /locus_tag="JZ382_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2427291.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRMDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRPLLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5987..6997)
                     /locus_tag="JZ382_001095"
     CDS             complement(5987..6997)
                     /locus_tag="JZ382_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY2427292.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLETAG
                     YTKDSYKEYIRNNLAFEAGLKAHVDITDDDLKTAWESFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAEDKSTDSETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEVSDV
                     ITATNTTSYATEYYVVKMVKNQNKGNDMDKYKDQLKEIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSGAKDSSEATSSTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7175..7522)
                     /locus_tag="JZ382_001096"
     CDS             complement(7175..7522)
                     /locus_tag="JZ382_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAY2427293.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLFAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLDHFKSALIETKETAESVIPAFQEAINKDIENFKFQAEPRIAQINEQVEK
                     LTADLPEYPDHIQ"
     gene            complement(7515..7949)
                     /locus_tag="JZ382_001097"
     CDS             complement(7515..7949)
                     /locus_tag="JZ382_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAY2427294.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFGNHQDSYGPEELKAIQETIVKQVHSDD"
     gene            8087..8824
                     /locus_tag="JZ382_001098"
     CDS             8087..8824
                     /locus_tag="JZ382_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427295.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFNVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEATKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDTAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGTLE
                     MLRKEFHLPDSSLDEIYIALTKEQAHE"
     gene            8817..10028
                     /locus_tag="JZ382_001099"
     CDS             8817..10028
                     /locus_tag="JZ382_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427296.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     SWLKTLHPPFALGGMIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPMILLILACGFAMPLVVVSTQQVFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKTGWLLSSMLILLLSFYWLVWAGVAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEQRLHQIYQFINLFTDVPEITAKVKRRRYLDPILTHIKKESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGVLIFGVIDFYFSLIIGSLFLYLIGFQLLPLASQFRYMVLVQ
                     LYPIHENQKKQAIQYLLTWVLVIASVIFGLIAAVVLDGQQRWVPLAAYLIVTGLFVKL
                     YVPTRLKKMSD"
     gene            10235..11014
                     /locus_tag="JZ382_001100"
     CDS             10235..11014
                     /locus_tag="JZ382_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAY2427297.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPPIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11083..11745
                     /gene="trmB"
                     /locus_tag="JZ382_001101"
     CDS             11083..11745
                     /gene="trmB"
                     /locus_tag="JZ382_001101"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY2427298.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFGNDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEIDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11854..13581)
                     /gene="ade"
                     /locus_tag="JZ382_001102"
     CDS             complement(11854..13581)
                     /gene="ade"
                     /locus_tag="JZ382_001102"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY2427299.1"
                     /translation="MESTLKRYIEVASKKKPADLVIKQAKIVNVFTKEIMEKDVAIYE
                     GMIVGIGEYEGKSVHNAKGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLQFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKNLAPFLQN
                     PEVLGLAEVMNYQAVANNEADILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPDNSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAISMGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLEDHLFADYSNPYMCCLPNLNAQPLHPRSLELLLPSSQA
                     HVIEIIPNSLVTNDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQKEMACLPLPIG
                     GIMTQEPFSFVNEQLNELIAETHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPC"
     gene            13713..14642
                     /gene="cpaF"
                     /locus_tag="JZ382_001103"
     CDS             13713..14642
                     /gene="cpaF"
                     /locus_tag="JZ382_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAY2427300.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYVLPASEVFYIYFRYGKKRTFW
                     KEVSSVDGQQLISRFKYLGQMDIGEKRKAQLGALTYSLEKQKVRLRLSSVGDYLQRES
                     LVIRLLYGISETPHRCFNSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALKEKR
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLIRIHELTKKKEELSECLAGVIYQELLEDADHHPSV
                     LWSYQFMEEEPVKKSWEESYEEAQKKTWQSELF"
     gene            14599..15648
                     /locus_tag="JZ382_001104"
     CDS             14599..15648
                     /locus_tag="JZ382_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion system protein"
                     /protein_id="HAY2427301.1"
                     /translation="MKKLRKKLGSQNYFKGLTRSQQNQFAQTMGELLLTGFSLQESLY
                     LLLTIRSFQPAILANIQHQLKEGQPFSEVLSQTGYGAEQLVQIELAELHGNLPATLLE
                     IAKQLRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLIVLPQLIGSGMVIEEHWGIQ
                     LIQVFHWYVLLFILMVTLLTLLVRYRLMKLSPIDRSEWISRQLVIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKMIQGLEAGIPLADQFQNYHFLSE
                     EFSKIILQGEAKGNLGKELIFYSEWTRKQLFKRLQHWLHFIQPIIFLGVAVLILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15645..15923
                     /locus_tag="JZ382_001105"
     CDS             15645..15923
                     /locus_tag="JZ382_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY2427302.1"
                     /translation="MKKKRTPYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRIPTVEELLNEGYIKREHADIYQRP"
     gene            15874..16368
                     /locus_tag="JZ382_001106"
     CDS             15874..16368
                     /locus_tag="JZ382_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAY2427303.1"
                     /translation="MKAISNENMQTSTSGLEGKTLASGYTLVESLLLILLLTTILSFP
                     VLAFSAWKNELAVYQFFSQFEKRIYTTQKIAIVNQIQTGFYWNIDENQIIFRMSQPDE
                     ASWKVLDIPDEITLKRHASITFAAGTGNESSLKAYQFYWEEKGQTITYQFQMGSGRYT
                     KRIE"
     gene            16361..16687
                     /locus_tag="JZ382_001107"
     CDS             16361..16687
                     /locus_tag="JZ382_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427304.1"
                     /translation="MNRKGFILLESLTALMIASVCVYLVSFGQLFLLQAEKNSGEEML
                     LIRMVYEDTKTQRIYHKRPERSEEMTMYDIQIVENNGVIEKVLVNMEKEAINIEKTRN
                     PGIYAD"
     gene            16704..17075
                     /locus_tag="JZ382_001108"
     CDS             16704..17075
                     /locus_tag="JZ382_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAY2427305.1"
                     /translation="MIVSSILLSFNLLIQQTIQVSDFLQRKDQKEWLIFLAQLEEELN
                     SSHNVSVKNQQLYYTVEDKQYIIERYQTMIRKRRTSGGHQPMLTSITEFQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17047..17406
                     /locus_tag="JZ382_001109"
     CDS             17047..17406
                     /locus_tag="JZ382_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427306.1"
                     /translation="MPYGQKTINRKCCKGSILFSVLLLFLLCTFLLTAALESYRLSID
                     LNIRIKNYYIAKIMKQMSLSEQSILAEKSEGIFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFIEEVKNDTDTGTE"
     gene            17578..18585
                     /locus_tag="JZ382_001110"
     CDS             17578..18585
                     /locus_tag="JZ382_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2427307.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGNQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKEPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGFGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQDLLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAKQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18610..19794
                     /locus_tag="JZ382_001111"
     CDS             18610..19794
                     /locus_tag="JZ382_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY2427308.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDSQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEGVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEDLKIITCHLGNGVSITAVN
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRNEIIKGMSWFGCEIDPALNELHGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20048..20518)
                     /locus_tag="JZ382_001112"
     CDS             complement(20048..20518)
                     /locus_tag="JZ382_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAY2427309.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAKEIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPPKKD"
     gene            complement(20591..>20792)
                     /locus_tag="JZ382_001113"
     CDS             complement(20591..>20792)
                     /locus_tag="JZ382_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427310.1"
                     /translation="VLIKKNDKNQGFLQFYGAAYAVLGVMGVLAAFFNQRFIALIFLL
                     IVILVSATFSIRFAKKIAEPKQ"
BASE COUNT         6852 a         3527 c         4174 g         6239 t
ORIGIN      
        1 tataggagac atggataaaa tgaaactaat tggtatagat ttagacggta ctcttttgaa
       61 ttctgaccag agcattagtc aaaaaaatgc aagaacaatg caacatttac ctaaagactg
      121 ttttccattc atttgttctg gaagagaagt agaagacatt aataatattc tcaaaaaatc
      181 tggtttaatg gttccagcag taggattaaa tggttctgtt ggatttgatg gagaaaaaaa
      241 aatatttgag tcttttttcg ataaaaagga tgtaaaaaaa attgattctg tcatatcgaa
      301 atttccaact aaaatatata cgaatctcgg aagttatgaa tcgcagaact ataaaaataa
      361 aatgaaaaaa atctttcacg aggtaggcag tgaattttct gtcaaggagt tgaactatga
      421 gcttgagtat gaaaaatcta tttgctctat tccttataat aatattgagg aaattataga
      481 aaaggatgat atcagcattt acaaattttt tatttttatt cccaatcgtc aattgaaagc
      541 gaatatattt aaccgtttga aaaagatgct aaatgtgact gttactgaat cagcacctgt
      601 aaacattgag atcgtgcctc aaaatgtatc taaaggcacg gtgtttgatc gtttggaatc
      661 aatctacaat ttaaaaaaac ctttgagaat cgcgatcggg gatagtttaa atgacttatc
      721 tatgtttgag agtgcaaata ttagttttgc aatgggaaat agtcatcaag agatcatgaa
      781 aaaagctgat tatattaccg tttcaaacga taaagacgga gtatctcaag cttttgaaag
      841 aattcttacc atataaacta atttagggag tatagaatat aaatatttag gattgtgaca
      901 aaaatcatgt cacaatcttt tttcgaataa actcattttt tgaagccgac tatttatcgt
      961 aatttcttta cataatacga acaaattttc ggttattgat gaaaaaaaac attgaaaatg
     1021 gtatgataaa agaaaaagag gagggaattt ttaatggtga aatttatcca tgcggcagat
     1081 ttacatatgg atcggtcatt tgaaggactg acaactctag ataaaactgt acaggaaaaa
     1141 ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat aataaataac
     1201 gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc attaaaaata
     1261 cagaaacatt tttcagaaca gatgaaacgg ttagaaaaaa atcatatacc tgtatttata
     1321 atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat
     1381 gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa aagtggagaa
     1441 actgtctctc tcagtggatt cagttatcga caaccatgga ttcaaaaaga caaagtgatg
     1501 gaatttcctt ctagagaact cacagattac catattggtt tgtatcatgg ggaacctggt
     1561 acctcgcaaa aagggaacta tgcccctttc caaccttcaa aaatgcaaga aaaaggatac
     1621 gattattggg ctttggggca tatccatgtg ccaacagttt taaatgaaaa gcaaaccatc
     1681 gtttatccag gtgcgcctca aggtcacact aaaaaagaac aaacttctac atccattcta
     1741 cttgttgaat taagtaaagg aagttgtcaa atacatccga ttaaagtagc agaggtctat
     1801 tggaagacaa aggaaatatc attgaggcaa gcgaggacga caaaagaggt cattgttcat
     1861 attcgtcagc aactttcaaa agtagaggat ggatttagtt taattgagat aaaattaaaa
     1921 gattatgacc atctcaatac agatgttcta gagcgaatac aatcaggaga gttattagat
     1981 tatctgttag aggaattttc agataggata agggatttat ttatttggcg tatttctctc
     2041 atggaggata ctttttatac gaagattcca cttgctgctt ctgctaaatt gatggaacag
     2101 ctatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga agtatatagc
     2161 caccaagaag cggctaaaat attgagcgaa ctgccggaat atcaagaaga aacacttgaa
     2221 aaagccaagc agctacttaa tcaagatttt ttatttgagg aggatcagga atgaagattt
     2281 taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgat ttttattcag
     2341 gcaatcagct gatctatggg ggaaatgaag caggaaaatc aacgatttat caatttatcc
     2401 aagccatctt atttggattc ccttcaaaag gccgcaaaaa aaaggattat acaccaaaag
     2461 acggctcagc ctatggtggc aaaatatggc ttaaacatcc tatatacggt gagtttgctg
     2521 tcgaacgcta taaacagcaa aatcgtggaa aatcgaaggt atggctgggg aatcaagttg
     2581 gaagtgatga attactagaa aaactgattg cacctttgac gaaagagtta ttccagcagg
     2641 tatttacttt ccagcaagaa cagttgatgg aactagatca cttgagagaa aaagagctac
     2701 atgatgcact gatttcttta ggaattacgg gcagttctca agtattccaa aaaagacaag
     2761 aatattatca gaaagcacaa cagttattca agaaaaaagg ccaaaaactg ctgatcaatc
     2821 aaaaattaaa tgaatggaaa gagcttcaag agaaaatcca tctaaaacaa gaacaggaag
     2881 cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag caacagttac
     2941 tggaacaact aaatgaggcg aataagcaat ttgcactagc cagacagcaa gaactcaatt
     3001 tttctttata tgaagaatgg caatccctca aaaaaaacat cgctacaggt cttcctgatg
     3061 aacaacttct tcttgatctg gagtcttttt ccaaacggta tcagcaaaca aatgagcgtt
     3121 tagaagaatt agaagcaact ctggaaaagc atagcgggat ggatcagcag tcagctagat
     3181 attattttta tttagagcag gagcaagaac taaaagacct ccaacaactc cagtttacaa
     3241 cggaacaatt aagcgaagaa tggcaaagac ttcaaataga gactagagaa acaaattcag
     3301 aattgaaaat cttggaacaa agatggcagt ggcagaaaaa tccgttaccg gaagaatttt
     3361 tatatgaaga agagtggaag aatcttctgg ctgaagaaag aaggattgct gatctagaaa
     3421 gccagatttc tgtgcgtctc cagatagcta aagaacagca aaaaatgatc gaagcagaag
     3481 tgaatgagtt ggaagagact tatccagcat tattacaaaa tcagtcagag atagcaccaa
     3541 ccccttcatc taaaccctat atgctaattg ctggcggagc aataatcatt gcaggtatat
     3601 ttgttccaat gcccttaaaa gtgatccttt tactaattgg tgcaggcatg attggctatt
     3661 tcttctacaa acagcaacca aaggtcaaaa ctaaaaatga tttctcagaa ataaaaggaa
     3721 tgtggcaaga aaaactaggt cagcttgatt tagctttagc ccaattagca gaagcagaag
     3781 aaaaacaaaa gagaatagaa gaagaaaaac agattcttca gcaagaagta tatcgttttg
     3841 ctgatcatcg gcacttagga aagatggata cactcaaact catgaaagaa tatgggcaag
     3901 aagtcataga ttatcagaaa ttgtatggcg tatttgtgcg gaaacataaa agagaacagg
     3961 aaatccaaga aaaactacaa cagatcgatc agaaatttga gtttgttaaa gaatggctac
     4021 ccatacaaga taagacgatc tcagaaaaaa tggctatgct ttcccgtttt atccaagaaa
     4081 tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag caagaaatca
     4141 atcaattgaa aaagaaacaa aaagtatggg tacaagaata cagcgaacaa ttgagtcaag
     4201 ctggattatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa caagccgaac
     4261 agcagatgaa aagatacaat gaactgtcag aaatgcttcg tccactgttt cctgaagaac
     4321 tgacgaaaga gaaattatct gtaagactgc ggcaactgaa agaaaaacaa ggccaactcc
     4381 agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa gtagaagagc
     4441 tgcaaagaga cggcacattg gacgaactct atcaacaagc tagccgacaa aaagcaaaat
     4501 tgcaggaatt gctggaggaa tggggcgcat ttgaaatcgc cggaacattc ttaggcgatc
     4561 tttcaacaga gatgagtgaa agacaattac cacagttgtt aaaagcagcg ggtgattatt
     4621 taggtatttt aacaaaagga aattatcaga agatattact gaaaaacgat acactgactt
     4681 taacagacgg atatacagac tttccaatct atgagttgtc tacaggtacg aaagatcagc
     4741 tgattatggc gatgaggttt gcttacctct cgttagaagc caatcgttcg atatgcccga
     4801 tcatcataga tgatggctgg cttcattatg atcatcagcg aaagtatcaa ttagctgaac
     4861 ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat aaagaaatgg
     4921 taagctatta ccatgagatg aaccaaaccg taaaacaatt agaaggagca aataatgaaa
     4981 aaaattcgtg atttagtaat cgatgagcag tttgagcaat ttgtcctgat caaaagtgca
     5041 gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca agatacttca
     5101 ggtactatcg atggcaagta ctgggatgct tcggaagaag aaatcaataa atttacagcc
     5161 ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aagggaaccc gcaagtaaag
     5221 attcttcaca tgcgtttaac aaggatggat gagccagggg aacctagttt atacatggaa
     5281 agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattatt atttgaaatc
     5341 acaaatgcgc attggaatcg gatcgttcgc tatctactca ataaatacca aaaacaattt
     5401 tttgagtttc cggcagccaa aagaaaccat catgcatttg ccagtggatt agcttatcat
     5461 actttgacaa tgcttcactt aggaaaatca attgccgatg aatatcctga gttgaatcga
     5521 ccattattat atgcgggtat cattttgcat gatttaggaa aggtgatcga actttccggt
     5581 cctatgtcga cagaatatac gcttgcaggg aatttaatcg gacatttggt attgattgac
     5641 gaagaaataa ctaaagcctg tcttgcgctg aaaatcagcg aagctgacga agatgttctc
     5701 gtattacgtc atatggtttt agcacatcac ggcctattag aatatggttc acctgtacgt
     5761 ccaaggataa tggaagcaga aatattacat caaatcgaca atattgatgc ttccatgcag
     5821 atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acacagatcg gatttttggt
     5881 atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat ttttttcatt
     5941 caaaacaaaa aagcgagaca taagtcttcg ctttttttgt tttcttttat tttgatgaat
     6001 cagttgtttc ttcagttgaa gattcttttg ttgaatcagt tgattttgtg tcagagctct
     6061 ttgttgaaga tgtggcttca cttgagtctt tagcacctga tgaagttgtt gtgaatgcac
     6121 tcaacacatt ttcaaaggca tcgtctttga tttttacatt tgcatctttt aattcttcac
     6181 cgattacttt agtcgtgaat gcattgtcac ttaattttgt ttcagtagcg atttctttta
     6241 attgatcttt gtatttatcc atgtcgttgc ctttattttg gtttttcacc attttgacaa
     6301 catagtattc tgttgcataa gaagtagtat ttgttgcagt aataacgtca gagacttcgc
     6361 cgtctttcaa tttgaaagct gcttctttta cttctgcagg aatggttgtt gtcgtcgagt
     6421 cgaatttgac tttaccgcca tcttcttttg tctctgaatc agttgatttg tcttcggcta
     6481 atttagagaa atcatcccca tcgtccgctg attttttgac atcttttgct tcatcttcac
     6541 ttgataattt gatgatttgt gcttctactt ctggatggaa agattcccaa gcagttttca
     6601 aatcatcatc tgtaatatct acatgagcct tcaaaccagc ttcaaaagct agattgttac
     6661 ggatatattc tttgtaagaa tctttcgtat acccagcagt ttctaattgt gattcaaaag
     6721 tatcacctaa agatttcgct tgtttgtcgt attctgcatc cacttgttta tctgttactt
     6781 tatcgccgta tttgttattg aatactttat agatgatcat ccgttgtact aatgattggt
     6841 tcgatgattc gagctttgct tcatcgtaga aatcagatac agtgatcgtt ccgcctttca
     6901 tcgttgcgat atctttattg gtatcgcctg aacaagccgc tagagttaaa acagccaacg
     6961 cactagtagc tgccaaaata attgattttt ttttcattaa tgtttgcact cctgtaatta
     7021 gttattattt tttatatatt aactatacca taccccatgc cctttaaaag cgttctcgtg
     7081 atagtttcaa acaaaattca aaatttcatc acattcctag gaaatgtgca attattcctc
     7141 tattagacag tcatatctgt tgagaaaatg atcattattg tatgtgatca ggatactcag
     7201 gcagatcagc tgttagtttt tctacttgtt cattgatttg ggcgatacgt ggttcggctt
     7261 ggaatttaaa attttcgata tctttgttga tggcttcttg gaaagcggga atcacagatt
     7321 cagctgtttc ttttgtctcg atcaatgcgc ttttaaaatg atccaagctg ttatttaaat
     7381 cgtttgtcag agtggttgct tcttccagtt cttcaatcag tttttttctt gtttcgttcc
     7441 cgctcctagg tgcaaatagc agaccaccga cagttccagc agcagctccg aatagcagtc
     7501 ctttgaaaaa gtttttaatc atcgctatgc acctgcttta cgattgtttc ttgaatagct
     7561 tttagttctt ctggtccata actgtcttga tggttgccaa agtggataga aaaatcgtcc
     7621 tctttgctat agcgaggaac taaatggata tgtgaatgaa aaacagattg ataagctaat
     7681 tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt ttctaatgct
     7741 tgagcaatct taggcacacg tgaaaaaact tctcctgcaa gttcaggatc atattcaaaa
     7801 atatctgcta catgacgttt aggaacaact aacgtgtgtc ctttcgttgc ttgggaaatg
     7861 tctaaaaaag catagacttt gtcgtcttca taaattttat agcttgggat ttcttgattg
     7921 atgattttgc aaaaaataca gttttccata aaaaacatcc tttccataaa gaataaatac
     7981 ttcagcttaa ctttaccata agttaaagcc attgtaagga aaaaaacaaa ataccgtcat
     8041 tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga gtttaattat
     8101 cgatcatgtc acaggagggt atggccatct ccctgtgtta aaagatatca gtttcaacgt
     8161 acagcctgga gagatagtag gattgattgg gctgaacgga gcagggaaaa gtacaaccat
     8221 caagcacatt atcggtctgt tggaagctac aaagggaacg atagaaatca atcatctatc
     8281 tttaaagaaa aatacagaag aatatcgtaa acaaattggg tatattccag agacgccttc
     8341 tttatatgaa gaattgactt tgagggaaca tatcgaagtc actgcaatgg cctatgatat
     8401 acctatggat acagcaatga aaagagctga aaagctgtta gagactttcc gtttgtctaa
     8461 tcgccttgac tggtttccgg ctaatttttc aaaagggatg aagcagaaag tgatgatctt
     8521 atgcgccttt ttgatcgagc cttctttata tatcattgac gaaccattct taggattgga
     8581 cccactagct atcaatgcgc tgcttgaatt gatggtagag atgaaaaaag aaggagccgg
     8641 tattttgatg tctacgcata ttttagctac tgcagaaaaa tactgtgacc gttttgtggt
     8701 cttacataac ggagagatac gagcagatgg aacccttgag atgttgagaa aagaatttca
     8761 tctgccggat tcttctttgg acgaaatcta tatcgcttta acaaaggagc aggcacatga
     8821 gtagtttttt tcaaaaacgt ttagcaaggc atcaaaagaa aatgatgaaa tatatgcgct
     8881 atgtgttgaa cgatcatttt gttttagttt gtcttttctt aattggtggc gtgggattgt
     8941 actattctag ctggttgaaa accttgcatc cgccttttgc acttggggga atgataatct
     9001 ctgtattttg gatgctctgt ttatttgtag gcaagatggc tacttttgcg gaggcagcag
     9061 atattgtttt tttacttccg aaagaaaaag aaatgcagac ctatttggaa cgagcttttc
     9121 gctattcttg catttttccg atgatccttt tgatattggc atgcggattt gctatgccat
     9181 tagtggtggt tagtactcag caagtctttt cgatgttttt cgtctatctg attatattat
     9241 ggagtttgaa attcagtcat ctgcaaatta agcgagcaga attgtttcgg ctagatcaac
     9301 aggtcattcg ttattggaaa acaggttggt tactttcttc tatgctcatt ttgcttcttt
     9361 cattttattg gctagtctgg gcaggggtag caggtgcatt ggtacaggca agtctatatt
     9421 actggttttt gtggaagcaa caggaaaaag gattggattg ggagaaaatg gtgcatgtag
     9481 aagaacaaag gttgcatcaa atctatcaat ttattaattt attcaccgat gttcccgaaa
     9541 ttacggcaaa agtaaaaaga agacgctact tggatcctat attgactcat attaagaaag
     9601 aatcaaaaaa cacttatctt tatttgtttg caagaagatt ggcaagaggt tctgatttta
     9661 gtggtcttta cttccggtta gtaattatag gtggggtatt gattttcgga gtgatcgact
     9721 tttattttag tctgataatc ggttctttat ttttatattt aatcggtttc caattgttgc
     9781 ctttagcgag ccagttccgt tacatggtgc tcgttcagct ttatccaatc catgagaatc
     9841 agaagaagca agcaatccag tatctgctca catgggtatt agttatcgca tcggttatct
     9901 ttggattgat tgctgcagtc gtattggatg gtcagcagcg atgggttccg ctagcggctt
     9961 atttgattgt tacgggtctc tttgttaaac tatacgtacc aactcgtttg aagaagatgt
    10021 cagactgaaa acaaagctgt gtaggataaa aatgaacatg aaaaatagag cgttgacagt
    10081 aagaatgctg ttcgttctat tttttttgag aaaagtcggt tttttgaatg gcaaataaat
    10141 gaaaaagaac gtatatgtgc ttgacgtgta gtgatcaggc gactagaata ataagtactg
    10201 caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag accaagagtg
    10261 gcggttacgt cccatcaaag gcgatacagg aaaaacatac ataggcatgc gtgatgaaga
    10321 caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa gggaaggaat
    10381 tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gataccttga cagcacaaga
    10441 atggctagaa ggccgcttgc taactcccaa tgagattggt caaagaaatg acgtgattga
    10501 tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc gtatcggcgg
    10561 acgtgttatg acgccggaga agatgttgca aaaatatgaa aaagaattac cagaagaact
    10621 ccagaaaaat caatttctgc aaatcgtcca tcgatttctt cgtgagcaga tcccgccgat
    10681 agataaggaa aatctatcag tggtgcatgg agatgtaaat catcgaaatt ggttagtatg
    10741 taaaaattat ttatatctcg tggattggga ttcaatcatg tttgcagacc ctgcggtaga
    10801 tattgcaacg attttaggga attatgtgcc tctatccagt tggaatcaat ggctgatcag
    10861 ttatggcata cgtccaactg acgaaatgct tgaaaagttt cactggtatg cggccatgaa
    10921 tttgctccaa gaaatcacta gatactgcaa acgtgaagat catcgacgaa tgaacgaaga
    10981 aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt
    11041 tggccagacc tttttaatta gagaaaggaa tggagataaa taatgcgtgt ccgaaatcgt
    11101 ccgggagcag cggaaatgtt agcggctcac ccgaattttg taattagtga tccaacattg
    11161 tggaagggga aatggaatga attgtttgga aacgatcatc caatccacat tgagattgga
    11221 atggggaaag gccagttcat tactggaatg gcaaaagccc atcctgaaat caattatatt
    11281 ggagtagaaa tgcaagtaag tgttgtttct atagcattag acaagttgat tgaacaaccg
    11341 cttcctaacc tgaagcttct gcatgtggat ggatcagcat tgacagaata tttcgctgac
    11401 agcgaaatag atcagattta tctgaatttt tctgatccat ggccgaaaaa acgacatgaa
    11461 aaacggcgct tgacatacaa aacttttctt gctgtagatg aacagatcct tcgcccaaat
    11521 ggagaaatcc acttcaaaac agataatcaa ggattgtttg agtattcgct ggcaagtttt
    11581 tctcaatatg gcatgatcct caaacaggta tggttggatt tgcatcaaag tcaatttgaa
    11641 ggcaatatta tgacggagta tgaagaaaaa ttttcttcga aaggtcaacg tatttatcga
    11701 gtagaagctc aatttcaaga taagaccaaa aagaaaagtc tgtgaatgaa ctttttactt
    11761 tcataaaaag ataaatccca gaaaacacat tacatctgtc atgtgcattt tctgggactt
    11821 ttttgattta agatatacct atttcctcgg ttttcaacaa ggtggaatca attggaatcg
    11881 agagaatgaa aataatcctt tgtcagtgat cttcaattcg ggtatgacag ataatgttaa
    11941 gaaagaaagt gtcaaaaaag gatcaaaggt tttagctgcc cctagttgat gtgtttcagc
    12001 tattaattca ttcagctgtt cattgacgaa cgaaaaaggt tcctgtgtca tgataccgcc
    12061 tatcggtaga ggaagacaag ccatttcctt ctgtttattg acaacagtca tcccgccacc
    12121 tttttgaatc aaaagattag cagcatacag catatcctca tcatttgtcc ccacgacaac
    12181 tagattatga gagtcatgag caatcgttgt cgctatcgcc ccttgtttca gctgaaatcc
    12241 ttttacgatg cctaacccaa tatttccggt ttgatggtga cgctcgatca ccgcaatctt
    12301 taattggtct ttttcgattg aaggaatgaa tcgttcgcct tgtcggtcca ctttttctac
    12361 caaatcgttc gtgacgagac tattcgggat aatttcgatc acatgggctt gtgaagaagg
    12421 gagaagcagc tctaacgaac gaggatgcaa tggttgagca tttaggtttg gtagacagca
    12481 catgtatgga tttgaataat cagcaaataa atggtcttcg agttgaccag attggacgac
    12541 caatgttccc ttatagaaaa cttcatgaat tggcagcttg gtaagatcgt ctgttaagaa
    12601 gaaatcagct tgatatccag gagcaatcgc acctagtgtt ttcaaatcaa aacattctgc
    12661 tgcattcaat gttgccatct ggattgcctg taatggatca attcccattg aaatagcttg
    12721 acggacgata tggtcaattg atccttcttt aactaaatca ttgatgagtt tatcatcggt
    12781 gacaaagaag catcttcttg aattatcagg cgtgatcgcc ggtagtagcg atgccagatc
    12841 ttttgcaacg gtcccttctc gaaccattaa atacatacca agatccaaac gttctttagc
    12901 ctcttttgca gttgtagctt catgatccgt ccgaatacca gctgcgggat aaacatttaa
    12961 ttcttccatg ccgataccag ctgcatgtcc gtcaatcttt ttcttatgct ggtgcatcaa
    13021 ttggatcttt tctaggatat ctgcttcatt atttgctact gcttgatagt tcatgacttc
    13081 agctaagcct aaaacttcgg gattttgtaa aaaaggtgct agatttttgg catttagatt
    13141 tgcaccattt gtctcaaatg gagtgacagg tacacaagaa gggagcataa agaaaatatt
    13201 cataagcgtt tgtcttgcgt cttctatcat aaattgaaga ccagtactcc cagcgatgtt
    13261 tcctatttcg tggggatctg ttactacagt agtaactcca tgaagcaagg atacttttga
    13321 aaattcactt ggtgataaaa gtgaactttc aatatgcaca tgtccatcaa tgaatcctgg
    13381 tactagatat tgccccttag cattatgtac gctttttcct tcatattcac caatccctac
    13441 aatcattcct tcgtaaatcg ctacatcttt ttccatgatc tctttcgtaa agacattgac
    13501 gatcttagcc tgtttgatta ctagatcagc gggctttttt ttacttgcta cttcgatgta
    13561 tctctttaat gttgattcca atggaatgcc tcccaagata aatttttata agtataacat
    13621 aaattcgttt tttgccaatg ttttttaata aattcatttt atttgaaaca aaaagatcaa
    13681 aaagacgtat gttttttgtg gaggggagaa taatggaaat aaaacgatta tcagatcaaa
    13741 tgataagtga aagtttcgcc aaacatgccc aagacgtata tgtcttgcca gcttcagaag
    13801 tattttacat ctattttcgc tatggcaaaa aacgaacgtt ctggaaggaa gtatcttcgg
    13861 tagatggcca gcaattgatt tcaagattta agtatttggg acaaatggat atcggagaaa
    13921 aaagaaaagc tcaattaggt gcattgacct attctttaga aaaacagaag gttcggttac
    13981 gactgtcttc tgtaggagat taccttcaac gagaaagctt agtgattcgg ctattatacg
    14041 gtatttctga aacacctcat cgttgcttta attctcgaga ttttgcaact attttgcaga
    14101 aaaccgttag acgtggatta tatctattca gtggaccagt cggatcaggc aagacgtcat
    14161 taatgtatcg gttggctttg aaagaaaaac ggcaagtaat cacgatcgaa gatcctgtag
    14221 agatcgaaga accattcttt ctgcaattgc agatcaatcc caaaatagca cagacttatg
    14281 acacactttt gaagctcgca ttacggcatc gccctgattt gttgattatt ggtgagataa
    14341 gagatgaatc aactgcacat gctgcggtaa gagctgcact aacgggacat cgggtattcg
    14401 caaccgtaca tgcacgcaat cttgaaggta cattgatcag aatccatgaa ctaacaaaga
    14461 aaaaagaaga gctatctgaa tgtttagctg gagtcattta tcaagaactt ttggaagatg
    14521 ccgatcacca tccttctgtc ttgtggagtt atcaatttat ggaggaagaa ccagtaaaaa
    14581 aatcatggga ggaaagctat gaagaagctc agaaaaaaac ttggcagtca gaactatttt
    14641 aaaggattga caagaagcca gcaaaatcag tttgcacaaa ctatgggaga gctgctttta
    14701 actggtttta gtttgcaaga atccctttat ttattactca cgatccgttc gttccagcct
    14761 gctattttgg caaacatcca acatcagcta aaggaaggac aaccgtttag tgaagtactt
    14821 tcgcaaacag ggtatggagc agaacaactg gtacagatcg aattagctga acttcacggg
    14881 aatctgccgg caactttgtt ggaaattgcc aaacagttac gtctggtcga aggatttcgc
    14941 aacgatttga aaaagctgct cgcctatccg atgctattgc tcaccttttt gctaggtatc
    15001 ttatttagta tgcgtttgat cgttttgcct cagcttattg gttcagggat ggttatagaa
    15061 gagcattggg ggattcagct gatacaagtc tttcactggt acgttctact atttatccta
    15121 atggttactt tattgacttt gctagtaagg tatcggctga tgaaattgtc tcctattgat
    15181 cggagtgaat ggataagcag acaattagtg attggaccaa tatattcctt atatcaatct
    15241 gcttatttgt cgctagagat tggcaaactt tttcatgaag gtttcgaatt aaggcaaatc
    15301 atcgtttgct tgaaggagac gaaacaagaa agtctgattc aacaccttgc ttccaagatg
    15361 atacaaggat tagaggcagg tataccatta gctgatcagt tccagaatta ccattttcta
    15421 tctgaagaat tcagcaagat tattttgcag ggagaggcaa aaggaaattt agggaaagaa
    15481 ctgatatttt acagcgaatg gacaagaaaa cagcttttca aacgacttca gcactggctg
    15541 cattttattc agccaattat ttttttgggc gtagcggttt tgattttact ggtttatgct
    15601 gctgttttat tacctgttta tggaagtatc gaggaggtct tgccatgaaa aagaaaagaa
    15661 caccttatta tagcggtttt acattaatcg aaatgctgat cgttttactg atcatcagtg
    15721 tactagtatt gttgtttgtc ccaaatttgt cacgttatag gaatcatgta gaccaagaaa
    15781 gtcgtgaagc aatcattcag ttggtagaca cacaaaaaga gctctacgcg cttcaaaaca
    15841 atggaagaat ccctaccgtg gaggaattat tgaatgaagg ctatatcaaa cgagaacatg
    15901 cagacatcta ccagcggcct tgaaggaaaa acattagcat caggatatac gttggttgag
    15961 tcactcttac tgattttatt gcttacaaca atcctttctt ttccagttct cgcattttct
    16021 gcatggaaga atgaattggc agtctatcaa tttttcagcc agtttgaaaa acgaatctat
    16081 accactcaaa aaatcgctat tgtgaaccaa atccaaacag gcttttattg gaacattgac
    16141 gaaaatcaga tcatttttcg tatgtcgcaa cccgatgaag cgagctggaa agtattagat
    16201 attcctgatg agattacctt gaaacgccat gcttccatca cttttgctgc tggaactgga
    16261 aatgaaagtt cactaaaagc atatcaattt tattgggaag aaaaagggca aacgattacg
    16321 tatcaattcc agatggggag tggacgttat acaaaaagaa ttgaatagaa aagggtttat
    16381 tttattagag tcccttacag cgctgatgat tgcttccgtc tgtgtgtact tagtgagttt
    16441 tggtcagctt tttttacttc aggcagaaaa gaacagcggg gaggaaatgc tacttattcg
    16501 aatggtttac gaagatacca agacacaacg gatatatcac aaaagaccag agcgaagcga
    16561 agaaatgact atgtacgata tccaaattgt cgaaaataat ggagtaatcg aaaaagtgct
    16621 tgtcaatatg gagaaagaag cgatcaatat tgaaaaaaca agaaatcccg gcatttacgc
    16681 tgattgaatg tctaatcgga ctaatgatag taagcagtat attgctgtca ttcaatcttt
    16741 tgatccagca aactatccaa gtaagtgact ttctgcagcg taaagatcaa aaagagtggc
    16801 tgatattttt agcacaatta gaagaggaac ttaacagcag tcacaatgtt tccgtgaaaa
    16861 atcagcaatt gtattacact gtagaggata aacagtacat catcgaaaga tatcagacga
    16921 tgatacgtaa aagaagaact tccggcggac atcaacctat gttaacaagt atcactgagt
    16981 ttcaattatt ggaaaaagaa cacacaattt ctttttatgt acactttgaa aatggagaag
    17041 atggatatgc catatggaca aaaaacgatc aatagaaaat gttgtaaggg atctatttta
    17101 ttttctgtgt tgcttttatt tcttttatgt acgtttttat tgacggcagc attagaaagc
    17161 tatcgcttgt caatagatct gaatatacga ataaaaaatt attatatagc aaaaataatg
    17221 aaacagatgt ctctatcgga acagtctata cttgctgaga agtcggaagg gattttttac
    17281 tacacaactg gatctgtaac atatcaatgg gtacaacagt ctctattctt ggaggtagaa
    17341 gtctcgccgt ttatctttcg attcatagaa gaagtaaaaa atgatacaga tactggtaca
    17401 gaatgagggg ttgttcgtga aaacgccctc ttttctgtta taacactgtt tttaataaaa
    17461 aaatgtttgc cgtttcacaa ataaaaaaca gtgaaaacga ttgaaatttt gtcaattgtt
    17521 cgatagaatt aaacaggtga aaaaattgtt ataatatatc tagaaatgag gtgcaccatg
    17581 ttaccagaaa aaatggaaca agggttcaat cagaacctgg aagctattca gcttttacag
    17641 aatgcattgg gaacttcctt tttagaagcc tatgtcgaaa atgcagaaaa tctaatagat
    17701 gattatcaag tacgtgtggt ggatggagtg ccgacaaaag agacaacaca acggatcaca
    17761 gctctttatg aagagttgaa aaaactttct ttcgagccag aagaatggcg cagattgtct
    17821 caattgcttt tattgaaggg caaccagaca gaacatctgc aggcgaacca tcaactcact
    17881 cctgatagta tcggtttttt atttgttttt cttattgaac aactatacac aaataaaaag
    17941 gaaccagtta agattctgga tattgcggca ggaatgggca acttgttatt aacagttctt
    18001 ttaaacctat cgaatgcggg gtatcaaaca gaaggatttg gtgtggatat tgatgataca
    18061 ttgttggctg tggcagcttc tacaagtgat ttgacacaag caaatgttca atacttccat
    18121 caagacgggt tgcaggattt attaatcgat cctgtcgatt ttgcaatcag cgatttgccg
    18181 atcggttatt atccgaatga cgaaaaagca aaagaatttt taacaagtac tgaagaaggc
    18241 cacagttatg cacatcattt attgctggag caatcaatga aatacgtgaa gccggatggt
    18301 tttggtttgt ttttgatgcc aagcggtttt ttagaaacgg atcaaagcga agaaatcaaa
    18361 aaatggttta aagaagaagg ctatcttcaa ggaatgattc aattgccaga tgagttgttc
    18421 cgcaacaaac aatcacaaaa aagtattttg atcttacaga aaaaaggacc acaagcaaaa
    18481 caagtaaaag aagtgttgct tgtgaaactt gcttcactaa aagaaccaga gaaggtgacg
    18541 gaatttttca acgagtttaa aaactggaaa tcatctgctc tgtaatcatt caacgagaag
    18601 gagagtcata tgtctaaaac aattgcaatc aatgctggaa gttcaagttt gaagtggcaa
    18661 ttatatcaaa tgccagaaga aaccgtagtt gcgaaaggaa tcgtcgaacg tatcggttta
    18721 caagattcta tttttacaat caagtacggc gatagtcaaa aattcgaaca aatcatggat
    18781 atcgaaaacc acgaagtagc agtaaaaatg ctgttagatc agttgattga actgcaaatt
    18841 ttaagttctt atgatgaaat caccggtgta gggcaccgtg ttgttcacgg aggagaaact
    18901 tatggggatt cagtagtgat cgatgaggga gtaatggacc gcattgctga attagcagaa
    18961 tttgctcctt tgcataaccc tgctaacctg atgggaatca aagcctttaa aaagatttta
    19021 cctgacattt tgagcgtagc tgtatttgat acatcttttc actcaacaat gccaaagcaa
    19081 aattatttat acagcttgcc agtgtcttat tacgaagatt tcaaagtaag aaaatatggc
    19141 ttccacggca caagccatcg ttatgtatcg gaacgtgcag caaaattatt gaataaaccg
    19201 attgaagatt taaaaatcat tacttgccac ttaggaaacg gagtatcaat cacagctgta
    19261 aatggtggta aatccatgga tacttctatg ggattcacgc cattagctgg tgtaacaatg
    19321 ggtacgcgtt caggcgatat cgatccggct gttcttccat acttaatgga aaaattagag
    19381 attgatattg atgaaatgat caatgttttg aataagaaat ctggtttgct aggcctaaca
    19441 ggattgtcta gcgatatgcg tgatcttgaa aaaaactatg aaaaagaaca cattcgttta
    19501 gcttatgata tcttcgtgga tcgcatccga aaatatatcg gcggatacgt gacaacaatg
    19561 aatggtgtcg atgcaatcgt ctttactgct ggtatcggcg aaaatgacgg acatgtacgc
    19621 aacgaaatca ttaaaggtat gtcttggttt ggctgtgaaa tcgatccagc gttgaatgaa
    19681 ttgcatggtg aagaagtaga tatctctaca aaagaatcaa aagtaagagt attagtgatt
    19741 ccaacagatg aagaactaat gattgcccgt gacgtggagc gtttgcgcgg ctaaaaatac
    19801 acgcgtgttc tctttagaaa atggataatt ttatatccgt tttctaagag gacatgcttt
    19861 tttatttttt acacagaagg agctgaatat aaagaagtga gtgaatccaa gtattctatg
    19921 aatcgtgaga ggaaagacgc tcaataagcg actttatttt ttgaagagca aaaaaagctg
    19981 tgtccagaca agtgatcttc aaaagtttta cttttgagat cccctctgaa acacagcttt
    20041 ttatcaatta gtctttttta ggaggcaatt ggttttctaa atcagttcga acaacaagca
    20101 catcgcaagc cgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta
    20161 cagcattcaa tcctgtagct cctaacataa tcaagtctac ttgattatct tctgggattt
    20221 ctttcgcaat aatttgtttt ggtgagccat attctacaac agaggttaca ttattcaagc
    20281 cagctttctt cgcgttgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt
    20341 gttctgccaa cattccatca aaagatgaaa cggtttggaa agaacgtgtg tcaatgacat
    20401 gtgctaatag aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta
    20461 attcagcttc atcagaacca tccacggcaa ccatgatttt tttgtattgt tgtaacataa
    20521 gcattcactc ctcaagtcaa tcgtgttgta ctcttttatt gtagaacaat ttgtggaaaa
    20581 agagaattat ttactgcttt ggttcagcaa ttttttttgc aaagcggata ctgaaggtgg
    20641 cagaaacgag aatcacaatc aataaaaaga tcaaagcgat gaagcgttga ttgaaaaaag
    20701 cggctaaaac acccataacg cctaaaacag catacgcagc tccataaaat tgtaggaatc
    20761 cttgattttt gtcatttttc ttgatcaata ca
//
LOCUS       DADJYG010000038        20209 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.038, whole genome shotgun sequence.
ACCESSION   DADJYG010000038 DADJYG010000000
VERSION     DADJYG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.038"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            77..3466
                     /locus_tag="JZ382_001114"
     CDS             77..3466
                     /locus_tag="JZ382_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY2427311.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3469..4890
                     /locus_tag="JZ382_001115"
     CDS             3469..4890
                     /locus_tag="JZ382_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAY2427312.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4887..6764
                     /locus_tag="JZ382_001116"
     CDS             4887..6764
                     /locus_tag="JZ382_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAY2427313.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6856..7698
                     /locus_tag="JZ382_001117"
     CDS             6856..7698
                     /locus_tag="JZ382_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAY2427314.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7850..8725)
                     /locus_tag="JZ382_001118"
     CDS             complement(7850..8725)
                     /locus_tag="JZ382_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2427315.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8932..9963
                     /locus_tag="JZ382_001119"
     CDS             8932..9963
                     /locus_tag="JZ382_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAY2427316.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10052..10357
                     /locus_tag="JZ382_001120"
     CDS             10052..10357
                     /locus_tag="JZ382_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAY2427317.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10536..12551
                     /locus_tag="JZ382_001121"
     CDS             10536..12551
                     /locus_tag="JZ382_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAY2427318.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12553..12837
                     /locus_tag="JZ382_001122"
     CDS             12553..12837
                     /locus_tag="JZ382_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2427319.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12863..14236
                     /locus_tag="JZ382_001123"
     CDS             12863..14236
                     /locus_tag="JZ382_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY2427320.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14319..16334)
                     /locus_tag="JZ382_001124"
     CDS             complement(14319..16334)
                     /locus_tag="JZ382_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAY2427321.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16356..17570)
                     /locus_tag="JZ382_001125"
     CDS             complement(16356..17570)
                     /locus_tag="JZ382_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2427322.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17791..18735
                     /locus_tag="JZ382_001126"
     CDS             17791..18735
                     /locus_tag="JZ382_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2427323.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19031..19975
                     /locus_tag="JZ382_001127"
     CDS             19031..19975
                     /locus_tag="JZ382_001127"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAY2427324.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         6895 a         3714 c         3857 g         5743 t
ORIGIN      
        1 attatgtaac gcctgtttat tttaataatt ttcactattc acgtcaaaac agaaaaaaag
       61 aaaaaggagg ggaaaaatga cgaccacagg gaagaaactg aaagttattt tcatgctgat
      121 aatattgagt ttatcaaact ttgtgccatt atctgcaata gcagacacta cagatgatcc
      181 aacagtttta gaaacaattt cagctgaagt catttcggat cagtctggaa aaaaagcact
      241 gaacatcaag ctaaatgcga ataacaccag tgctgaaaag atagaaaaag aaattggtct
      301 agtcgaaaat tacttaagtg atgtggaaag aaaagaagga gatggctatg cttatcaggt
      361 aaatagcggg aaaattacgt tggaaatctc atcaaacact aaacaaacta tcgatctgag
      421 ttttccaatc gatccagcac tttaccacag ccaggcaaac aagctgatcg tcgataataa
      481 agaatatgac attattgatg agacagaaaa taagaaagat acagatgtgt cagtaccaaa
      541 gccagacgaa atagaagaag aatcatcaaa agaaaacgaa aattctgtca gcccatttac
      601 attgcctaca ttatccttgc cagctgtgag tgtgccatct aatcaaacga ttcctacaga
      661 atatacaaca gatgatcagg gcacttatcc taaagccagt tggcaaccta caggaaatac
      721 aaatgttctt gatcatcaag gcaataaaaa cggaacaaat caatgggatg gtataaattc
      781 ttggaatgga gatcctaatg atcggaccca ttcgtatatc gaatatggag gaaccggtaa
      841 tcaagcagac tatgcgatac gaaagtatgc aaaggaaaca agtacacccg gattgtttga
      901 tgtttatttg aatgctcgtg gaaatgtaca aaaagatatc acgcctcttg atctcgtatt
      961 ggtcgtagac tggtcaggaa gtatgaacga caataatcgg atcggtgaag taaagattgg
     1021 tgtcgatcgt tttgtcgata ctttagcaga tagcggtatc acagacaaaa tcaatatggg
     1081 atatgtcggc tactcaagcg aaggatatag ctacagtaac ggtgcagtac agatgggttc
     1141 atttgattca gtgaaaaatc aagtaaaatc cattacacct tcacggacaa atggtggtac
     1201 ttttacacaa aaagcactaa gagatgcagg aagcatgcta tccgttccaa atggacataa
     1261 aaaagtgatc gttttgctga cggatggtgt accaacattt tcctataaag tacagcgggt
     1321 acacgcacaa tcaagcagca attattacgg aactcagttt tctaatacgc aagatcggcc
     1381 gggaaatact tctctaatct caagaatcta tgatgcacct gaccaaaaca atctatccag
     1441 aagaatcgac agtacgttta tcgcaaccat cggagaagcg atggcactca aagaacgagg
     1501 aatcgaaata catggtcttg gcatccaact tcaaagcgat ccggcagctg gtctctcaaa
     1561 agcagaagta gagtctcgta tgcgacaaat ggtttcatca gatgaaaaag gcgatcttta
     1621 ctatgaatca gctgatcatg caacagatat ctctgaatac ctagccaaaa aagctgtaca
     1681 gatctcagca actgtaagca atggacaaat aaatgatcca atcgcagaac cattcattta
     1741 tcagcctggt acactttcag tcaagagtgt ggggacaagt cctacaacgg tcactccatc
     1801 tatttccata gaaggaaata ccatcaagag caatcagatc tatttaggaa aagaccaaga
     1861 aatccaaatc cattaccaag tgagaatcca aacagaaaat gaggacttcc atccaaattt
     1921 ctggtatcaa atgaacggca ggacaacttt ccagccaaac attgatacca atgaattagc
     1981 tgaattcggt ataccatctg ctaaagctcc cggagtcagt cttcacatca aaaagttatg
     2041 ggaagaattt gacaacaatc tagctgatcg tccagatcaa gttacttttg agattcaacg
     2101 ggaacatacg acaaatgctg cagcttggaa aaacggatat attcgaatca ttaaaccagc
     2161 taaagataca acaaatacgt gggaacgtgc agacattgac aaattatctg caaatagcgg
     2221 agaaagttat caagagatat tatcactacc tcaatacaat aatcaaggtc aagcattcag
     2281 ttaccaaaca atcaaagaat tacctgtacc aggatacgat tctcaacaaa tagatgcaat
     2341 gacatggaaa aatactaaac aattcacacc gttaaacttg aaaataacga aaaattcctc
     2401 tacaggtgaa aaggatctta ttggcgctgt tttcaaatta acaggagatt ctattgatac
     2461 tttactaaca gatcatggcg acggaaccta ttctcttcca gaaaatgtca aattgcaaaa
     2521 agaaatgacc tatacgctga cagaaacaaa agctccagaa gggcatggat taagcaaaaa
     2581 gactacttgg gaaatcaaga tcgcttctga tggtacggta accattgatg gaaaaacagt
     2641 cactacttcc gatgatacga tccagttgac tattgaaaat ccttttgttg aagttcctgt
     2701 agcagtacgt aagtatgcga tgcaagggac ggacaaagag ataaatctta aaggagcagc
     2761 attttcccta cagaaaaaag aagcaaatgg tacttatcag ccaattgaca gccaaacaac
     2821 gaatgaaaaa ggtcttgcca gttttgattc actcacacct ggtaaatatc gagtcgttga
     2881 aacagctggt cctgccggat atgatacttc gccgggaaat tatgaattcc aaatcgataa
     2941 atatggaaaa atcatttaca cgggaaaaaa taccgagatg acaaataatg tatggacgct
     3001 cactcatcaa aatcgactaa aagcgtttga tctaacggta cacaaaaaag aagacaacgg
     3061 acagacatta aaaggagcaa aattcagact gcagggacca gaaatggact tagaatcgcc
     3121 aaaagatgga caagaaacag atacctttct attcgaaaat ttaaaacctg gaacttatac
     3181 gctgaccgaa acttttacac cagaaggata ccaaggtcta aaagagccag ttactatagt
     3241 tatacacgaa gatgggtcaa ttcaagtgga tggacaagat catgaatctg ttctgtcacc
     3301 aggagccaaa aacaaccaga tttctttaga catcacgaat caggcaaaag taccattacc
     3361 tgaaacggga ggaattggcc gtttaggaat ctatctagta gggatgattg gttgtgcgtt
     3421 ttctatttgg tatctttttt tgaaaaaaga aagagggggc agctaaatat gaaaaaactt
     3481 ggttggctta gtatgtgtct cttcttgtta ctatttaaac cagcttttac tcaggtagca
     3541 acagaaacag aaacagaaat ggttcagatt actttacaca aattgctttt cccaaacggg
     3601 caactgccga aaaatcatcc aaatgacgga caagaaaaag ctttattaca aacgtatcga
     3661 ggattaaatg gtgtcacatt ccaagtttat gatgtcacag attcttttta ccatctacgg
     3721 gaaaagggca aaacggtaga agaagcacaa gcagagatcg caaaaaacgg tgcgtcttcc
     3781 ggtatgttta ccgcagaagc aacaactaca actcttaaca acgaagatgg tatcgcttct
     3841 ttttctctgg ccgctaaaga tcaagaaaaa agagataaag cgtatctttt cattgaatcc
     3901 aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg tagttgttct tcctgtacat
     3961 ggacaaaaca atcaaaaact ttcaactatc catttgtatc ctaaaaatga agaaaacgac
     4021 taccctgatc caccttttga gaaggtatta gaagagccta gaaatgattt tacgattggt
     4081 gaaaaaatca cttattcctt gcatacgaca attcctgtaa atatccttga ctatcaaaag
     4141 ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt tacctaatag tttaacgatt
     4201 tcatcgaatg gagaaaagct gacagaaggc tttgtcatac acaagaaacc tcacggattt
     4261 gatgttttat tttcgatccc ttcgttggaa aaatatgctg gaaaaaaact gaccatttct
     4321 tatcagatgc agctaagcag tacagcacag gcgaacaagg aaatcaacaa caacggaaca
     4381 ctggattttg gttttggtgt cagtacaaag aaagtctctg tatatacagg gagtaagcaa
     4441 tttgtcaaaa tcgagacaaa taaaccagat aaacgattag ctggcgcagt attccttatt
     4501 aaaaacaaag caggaaatta cctccagcaa acagccaacg gatacaagtg gacaaagaac
     4561 gaatcagatg cgcttcacct gatttccgat aaaaatggcg ctttttcaat ttccgggttg
     4621 aaaacaggaa gttatcgatt aaaagagatc gaagcacctt ctggttatat tttaagtgaa
     4681 acagaaattc cgtttaccat ttcaactttt ctttctgagg ataaagaggc ggacagtata
     4741 ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc ttccaaaaac aaacgaaacg
     4801 aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa gctttgcaat ctggttgttt
     4861 atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa aaaaataaac gttatgttag
     4921 gagtcctttt ccttatttta ccattactca caaacagctt cggcgcaaaa aaagtgtttg
     4981 cagaggagac agcagctcaa gtcatccttc ataaaaagaa aatgactgat ttacccgatc
     5041 ctttaatcca aaacagcggg aaagaaatga gcgaattcga tcaataccaa ggattagccg
     5101 atatttcatt ttcagtttat aacgtcactc aagaatttta tgcgcaacga gataaaggag
     5161 cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac tcctggtaca ccagttgctt
     5221 caggaacgac agatgctgat ggaaatgtca ctttatcttt acctaaaaaa caaaatggga
     5281 aagatgcagt ctacacgatc aaagaagaac caaaagacgg agtgtcagct gccgcaaaca
     5341 tggttttagc tttccctgta tatgagatga tcaaacaagc agatggctct tataaatacg
     5401 ggacagaaga actagatact atccatctct accctaaaaa tacagtcggt aatgatggaa
     5461 cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc actaaatgga gcagaattta
     5521 ttatttctaa agaagaagga acaccaagcg tcaaaaaata catccaaagt gtcacagatg
     5581 gattgtacac ttggacaact gatcaaacca aagccaaaca tttcattact ggtcattctt
     5641 atgacatcgg caacaatgac tttgccgagg catctattga aaaaggccag ttgatcgtta
     5701 atcatttaga agttggaaaa tataatttag aagaagtaaa agctcctgat aatgcggaaa
     5761 tgattgaaaa gcaaacaatc acgccttttg agatcctggc aaatagccaa acaccagtag
     5821 aaaagaccat caaaaatgat acgtctaaag ttgataaaac aacacctcaa ttgaatggaa
     5881 aagatgtcgc aatcggtgaa aaaattcaat atgagatttc tgtcaatatc ccattaggta
     5941 tcgctgataa agaaggaacg caaaacaagt acacaacatt caaacttatc gatactcatg
     6001 acgctgcttt aacatttgat aatgattctt caggaacgta tgcttatgcc ttatatgatg
     6061 gaaataaaga aatcgaccca gtaaattatt ctgtcactga gcaaacagac ggattcacgg
     6121 tttcagttga tccgaattat attccttcat taactcctgg cggtacattg aaattcgttt
     6181 actatatgca tttgaacgaa aaagcagatc caaccaaagg attttctaac caagcaaatg
     6241 tcgataacgg gcatacaaat gatcaaacac caccgtcagt cgatgtcgtt actgggggca
     6301 aacgatttgt taaagtagat ggtgacgtta catcagacca aacacttgct ggagcagaat
     6361 tcgtcgttcg tgatcaagat agtgacacag cgaaatattt atcgatcgac ccatccacaa
     6421 aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt ttttacaacc acaagtaacg
     6481 gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta tctggaagaa acgaaagcgc
     6541 cagaaaaata tgttccatta acaaaccgtg tagcatttac tatcgatgaa caatcttatg
     6601 taacagcagg acagttgatt tctcctgaaa aaataccaaa taaacacaaa ggtacacttc
     6661 cttcaacagg cggtaaggga atctatgtgt atatcggtgc aggagtagtc cttctactga
     6721 ttgctggact gtactttgct agacgcaagc acagtcagat ttagtctttt tgcaagaatt
     6781 ggaaaaagga gcagcggatt acgcaaatgc tcctttttct tttattatca aacagtcatc
     6841 acaaggagaa cacaaatgaa accaaagaaa aggaaacttc tagatctgct gatgcttttc
     6901 ttgcttctct caggaatagg gatactagct tatccatttg ttagcgacgc gctgaataat
     6961 tatctggatc agcagatcat ctctcattat cagcaacaag cggtcaaaga aaatgaagaa
     7021 gtcatggcta aaatccaaga aaacatgaca aagaaaaacc agcaattagc aaaagaaggt
     7081 ggaaatccag gtgcagatcc ctttactaag aaaaaagaac ctgcgaaaaa ggatagaacc
     7141 tattttgaga aacatacgat cggtattttg acgatccctg agatcaatgt ccatttgcca
     7201 atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg cctcattatt ggaaggaacg
     7261 tcttatccta cggggggaga aaatacacat gccgttattt ccagccatcg aggactgcct
     7321 caagcaaagt tgttcacaga cttaccgaaa ctgactttag gtgattcttt ctatatcgaa
     7381 atcaatggac ggacactcgc ttatcaagtc gatcagatca agacggtaga accgactgat
     7441 acaaaagatc tgagaatcga aaaagggcag gatttcgtga cactcctaac ttgtacacct
     7501 tatatggtca acagtcatcg cttacttgtc agagggcacc gggttcctta tcatgcaaaa
     7561 gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac gatttttcct gtatggattg
     7621 ttagctgcat gtatactgat tttcctcctt cttatatatc tttttcgtca atcgttcaaa
     7681 ataagaaaca gaagttagct attcctcatt tctataatat atctattcga atcgttccgt
     7741 aaacaaacag gctgaggttt gaaaaagaaa acttcagcct gtttttatga tgatatcaaa
     7801 gagagcctat caaacactat ctttcttatt actaaatgag gatcttttct taagttttcc
     7861 gatattcttt aagaaggcct ttttgcatca attcttgttt gtttttcaag ttgcggatat
     7921 tctcttcaaa attttgagaa tatatcactg agaataacgt atttaatacg taatcgaagg
     7981 caatttgaga ggagaaagtc gctactttag caaaatcata ttcctcctgc gatataacaa
     8041 gacacatatc agcctgtttg gccatttctg aatgctgatt tccggtaatc agcaaggtag
     8101 gggcctctac atgcttcaaa taggtcatta ttcgttcata atgatggatg cgaccgctgt
     8161 agctaataaa aatggcgcaa tcctcgctag tcaaattggc tgcattccaa gaagaatcac
     8221 tgtattcttc agccatgatc aaaaacttat tcaacttgac cattttattc tggaatttcc
     8281 ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat tcgctgtgcc ttgttcaata
     8341 ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa ttgtgctttt tttattgact
     8401 ggatcgataa atccgccatt ttctttgcaa tttgctgagt gctatcttca cttgaaaaag
     8461 gaaaattagg atcaatcgtt tgattgaact gagcaatcaa ttgatgaact tcccgcccca
     8521 gctcgaaacg gaattcttta aatccgctga atcccatttt tttgcataaa cgaatgatag
     8581 ctgaatggga agtatacgta gcttttgcta attcttcaat cgtcatgtag actgcagctt
     8641 cgatattcct acggatgtaa tcgacaatcc gtttctctgt cgtggtaaac aactcttgtt
     8701 ttgttagttt ttcttcaata agcatgatct gacctccaaa ttctatctta ctgataaaca
     8761 cttggttccg ctactatctt tttgattttt atccttactt tgaacattaa tttcaccaaa
     8821 ccgaatgata acaagtgttt ttctgaactt ttttaccaga atagctatta taaatacttt
     8881 ctgcgctttt ctaggatata ctgaaaacat caataaaagg aggcaccact catgcttaca
     8941 atcgcttatc ttggcaatgg aaagagtaca aatcgctatc atttgccttt ttcaagaaaa
     9001 cttacgaaca aaatcaatat caaaaaaatt ttttccccat ctgaaccagt gtggaaaaca
     9061 ttcgaagaca tcgaatatac tgatcagctg tctgatcttt gggatgatcc tgagatccaa
     9121 ttagttgtca tcactacacc tagtcaattt cattatgaat atggaaaaat ggcactcgaa
     9181 catggtaaga atgtactagt agagaaacct tttgcagaaa cgtcaaagga agcagaagag
     9241 ttatttgcat tagcaaaaga aaaaggtcta tttatccaat gttaccaaaa cagacggttc
     9301 gattctgact tcttgacagt ccaaaaagta atcgaaagcg gaaaattagg ggatatttta
     9361 gaagtagaaa tgcattatga ttatttcaga ccggaagtac cagaaaatac gcatgaattt
     9421 tctactgcga acagctattt atatggacat gcttgtcata cagtagatca agttctttct
     9481 tatttcggcg aaccagatga tatcagctat gatgtacgtc aacttttagg gaaaggacga
     9541 atgaatgatt acttcgatct ggattttttc tatggaaata tgaaaatttc gataaaatcc
     9601 agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt acggaaaaaa agggatgttc
     9661 gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa tgttttacat gcctgatcat
     9721 gaagactttg gtatcgattt gcccgaacat tatggtgtat tgacctatat agatgaacaa
     9781 gggagttatc atgaagaaaa agtaatttcc gaggttggtg attacagccg tgtctacgaa
     9841 gggatttacg agacattagt taatggcgca gaaaaagtca tcaaagatga agaaacaatc
     9901 ttacaaatga aaattttgga agaaggaatc aaaagcattc aagaatcaga aggaaaaata
     9961 tgacatattt gtaagaatcg ggtttgtttt ttgacgcata aactgtctta ttgtacgatg
    10021 aaaaagtata gaactactag gaggaaccaa catgacaaat tatgacggtg aaaaacacaa
    10081 acgattaaga ggttcaatgg cccctaaaac agaagaagaa gtggctttag caggaactaa
    10141 ttctggtgaa acagaagaca aagaagaatc attagctggc acaaactctg gtgaaacaga
    10201 aaataaagaa acagctttat ctggttcaaa ttctgtagaa gaggaaacaa agcctaatga
    10261 gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa gatgtcaaag aaaaatttga
    10321 agaactgacc tcaaaaaata aacatgatca tcattgatcg gacgaaatca attcataaaa
    10381 gcaggagaca tttggtcacc tgcttttttt ctgtatccat tcgatagaaa tcgttcggta
    10441 cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac ccctcgattt aaaagcgcta
    10501 ccatactaga atataagtag gaggtgaaca gcgctgtgaa ctacaaaatt agttatctcc
    10561 tgaaaacacc aagagaaatc agtctagaaa aagcagcaga gatcatcgaa gtaaatacaa
    10621 gcgaggcgat caaacaatta actccttttg ccgaatatgt ggatgatacc cgtatccgta
    10681 taaagaaatt ccgagcaatc gactgggcag aaaaattttt ggatattact cgatttgaag
    10741 ctgtgtatac cgaacaagag cgaagagatc ttctttattt gatggtttat tcgactgcaa
    10801 cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt ttctaaaggg acagtcctgt
    10861 cagatatcaa aaagataaga aatgaattag aagtggaaga ttgtcagctg tcgtatacta
    10921 ggaaaactgg ttttaggctg ctagggtcag aagcaatatt aagaagagaa gcaaaaaatc
    10981 gcactgctcg tttgatgcag tcacttgctg gaagatttgg gctcacgtat ttaaccgcaa
    11041 ccctttccat tgattattac gccaaagtta gagatttgct agataaaacg atttcactat
    11101 cagatcttcg attcgcacca aatcgaatgg atgaattagc ttattatttg agtttgacaa
    11161 ccattcgaat tggaaaaaca tccatggaac tggtaaaaga tgctgaactg ttggcttctc
    11221 ttgcagtcac tgacattagt cgacaagtat tgactggcct tggctattcc gaacatcaaa
    11281 attcggagat tatatttttc tctctttgct tagcatcgat cgttgaagga gatgtccagg
    11341 agcctgcact tgattttttg ctggaatgtt cttcagaagt catccatcgc atggaattcc
    11401 tgatggccgt agaatttgct tcgtttcgtg aattattgat gaatgtcttt tatcacttgg
    11461 tccctgccta ttttcgtatt tcctattctt tttatttacc taatgtgatg atcgatcaaa
    11521 ttaagcatca atatgcttcc atctacgaaa tgacaagaaa agcattactt ccgttagaaa
    11581 gacgaatagg gaagagcatc cctgaagaag agattggttt tttcactata ctatttggcg
    11641 gagagatcag aaaagcggac gcagaagaaa gaaatagaaa aataagagcc gtgatcgtct
    11701 gtccaagtgg cattagctct tcattgatct taaaaagtga acttcagcaa ttattcccga
    11761 tgatcttttt tacagagact aattcagctg accgcttaca cgaggttgac gaaacttctt
    11821 acgatattat tttttctact gtacctctca cacttactga taagaaaaag tctctctatg
    11881 ttctgcatcc aatcatgact gacttggaaa aaaatcagct gatcaatcaa gtacaaagag
    11941 attggctgat accaggattt tcaatgcctt ctgcccaaga attgttgtcg gcgctactgc
    12001 cttatatcga attaaaagaa ggcgtagacg aagaaaagct ctatcaagta ttaaatcgta
    12061 aaatgaacaa acttttagaa aagaaggagg atcaacgtcc tatgctatct gaattattaa
    12121 cagaagaaat gatccaatta tcggatcaac caaaaaactg gcaagaagca atcacatttg
    12181 ccgcacagcc gctattatca gaaaataaaa tagaagaaag ttatatcaaa gctatgatcg
    12241 agcgcgtgga gcagtatggt gcgtttatcc atattggcga tcacatcgca ttacctcatg
    12301 ctcgtccaga agatggcgta aacgaagtag ggatgtcttt gctgaaactt caacaacctg
    12361 tggatctgct tgataatcca gcccatccca ttagtttgtt catctgttta gctgctatcg
    12421 ataatgaggc acatcttcgg gctttggcaa atttaacaaa gctactttct aataaacaaa
    12481 atcttcaaga tttattacac gcatcaacaa aagaagaaat attagcgatc ataagaaaag
    12541 gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg gattaggaac aagttttatg
    12601 gtagagatga acatcaaaca agcacttgca gatcttggag cagatgtgga cgagtttgaa
    12661 gtaagtcata cggatgttgg aagtgtatct agcgatatgg ctgattattt cttcattgag
    12721 cattctttga aagatacgct aacttctata cctgatgaaa agctcgtacc tttgcaatcg
    12781 atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga tcttgtcaaa agaataaaaa
    12841 tgattcaagg gaggaactat caatggataa attgttgaat ttgtttgtgt caatcgtcag
    12901 tcagccctcc atcctcgttt ctttgattgc actgcttggt ctcgttcttc agaagaagaa
    12961 attttcagaa gtgatccaag gaactatcaa aacatttgtc ggattcctag tactgatcgg
    13021 cggtgctggt ttaatcagta attcactaac cccttttgct tcgatgttcc aaacagctct
    13081 gcatactcaa ggagtagttc ctagcaatga agcagtagtg gcgattgctt tgcaacaata
    13141 cggtacacct actgctttga tcatgctagt agggatgatc gtgaatattt tacttgctag
    13201 attcacaaga tataaattca tttttcttac aggtcaagca atgctttatg tttcttgtct
    13261 aacagcagtc attttagtaa gcgcggggtt tactgcaaat ttgtggatga tcttattagg
    13321 cggggtcttc gaaggaacac tattaactat tacgcctgct ctttgtcaac cgtttatgaa
    13381 gaacatcact ggaaacaatc aagttgcgtt agggcatacc ggaaatatcg gttatgcttc
    13441 tagtggatgg atcggaaagt tttttggaaa caaagaccgt tctacagagg acataaagat
    13501 tcctaaaagt ttcagctttt tgcgagattc tactgtatcg atcatgctct tgatgtcgat
    13561 cgtatatgtc gcacttgctt tgttagctgg tcctggattt gttgaaagag aactaagcaa
    13621 tggagacaac gctgtaatct ttgcactgat ccaagctgga acattcacag ctgggtttgt
    13681 tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc gttccagcat tccaagggat
    13741 tgctaaaaaa ctcgtacccg attctaaacc agcattagat gttcctatcg tttttccata
    13801 tgcacctaat gctgtattaa ttggcttttt tgttagcttc tttgtaggaa cactatccat
    13861 gtttgctatg ataggcttgc aaactacagt tatcattcct ggggtagtag gacatttctt
    13921 ctgcggagct gcttctggtg tcttcggcaa ctcgactggc ggtagaagag gggcggtatt
    13981 aggttctgct ttcaatagct tattgattag ttggctgcca ttatttatcc ttccagtatt
    14041 aggagatctt aaaatcgctt cttctacatt tgccgatact gattatctag taccaggact
    14101 tgtacttggg aaactaggag aatacggtca aatagccttg acagttggaa tcatcgcttt
    14161 ctttttactt gtcgtattat ctagctttct ttttgccgga aaaagaacaa acattgtttc
    14221 taaagaacag aattaagcaa acttcaaaat aaaaattgct tggacaatat cagaagaaga
    14281 tttaaagtct tctgatatgt cgcaagcagt tttttgtttt atgcatttat ttttgaatag
    14341 cttgttaaga caagtggctg ttttgctaga agaggataac ttttaccatt cattttcact
    14401 gttccgcctg ttctatcaga agtaatcacg atcttatctt ttgttttttc aagcgaaatc
    14461 tttccatctt tgataggtag cgtacaattc agtgtttcaa atgaatctag attaggttgt
    14521 atcataaatt cttcataacc tggctttgtt ggatacaaac cgataaaata gcgtcctaaa
    14581 agatagattg ggcttgctcc ccatgcatgg cataagcttt ttccataagg atctccatac
    14641 atactgtact tttcatttcc tgtttgagta gggtcgtatt cttcccagaa agttaccgca
    14701 tcatgagata acatccctcc ccaataatca agaatcgttt ggtagacgat attcgtctgt
    14761 ccgagtttgc ataaagcatc ttgttcatag aatttgaaat atggagtagt aatctgtgtg
    14821 atagtgtcat tcaataagac attctttaag atcgattgct gttggtcttt gttagcaaaa
    14881 tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat ttcttctccc agattcatag
    14941 caatcgataa aagcgccaag ttcttcatcc caaaagtagt caaataggtt ttgtttcaat
    15001 agttgatatt tttcttgata gagatgagta tgataatttt cgttctctcc atatgtttca
    15061 gctaagatat ctccgcaagt gatcatggaa tgataggctt tcagtaataa gacttgttct
    15121 gcagcaagcg ttccttcttt atccatttca gaccaatcaa taaatatcca atcttttgct
    15181 ctcccataaa taaaccctaa ttcattcgtt tgctccatca agtatctcat taatgattgc
    15241 atcttagggt agacgatgcg taaaaattca tagtctttcg tcatcatata gtggttttcg
    15301 ataccaatca accacaagat cgaatagtca acgatagtat tcatatgctg cttgatttca
    15361 ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt cctcgtcaaa aaaaagatat
    15421 tgattgatca tgaatgattg atacgcatct ccagaccaaa tccagcgatc tcgtttgata
    15481 ccgtcgataa aaaacaaatc actacataac ttgaaggctt cttctgatac tgaccagatc
    15541 tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt tcgaaagaga taaaaattca
    15601 tgaaaaactt gaagttgaac cattccagta gaaacctctg gtataaacaa atagcgaaaa
    15661 gcgcgtttac gagctttcat ttttcttgtc attcctattt gcttatagta gcaataatcg
    15721 atatccatag cttctacatc ggactctcca taacatagag tgatagattc gatatggtca
    15781 tccagagata ttagttctac acagccattc actgctcgcc caaaatcgaa taagattcct
    15841 ccgccaatct ccgtttcagt gactggatgg acgcattctt tttgataata gatttgattt
    15901 ggatcttttg cgatatcgac ataaagcgga ctccagccag ctggatattc ttcaataaaa
    15961 ttacttgctg tccagccaat atctgatttt acttcgtctc cctcgataaa catcgctggt
    16021 agccctgatg tatgtcctac aaagatacga atttttgctt taccagctgg acaagtcagc
    16081 cattttccac atggatgttt ttgtccatta atttctacat acccaactcc ttggatcgtc
    16141 actttaaaca tggaaggttg atcaagaaag taatatctct taaacttgac gttccgatgg
    16201 caatcatcca tgtaccagta cgctggccag tcaaaacctc gttcttcccg ttggaaattt
    16261 tgtagcagtc catggcggat ctcaaagtct ccaggatacc aaagccatct atctttatgt
    16321 ttttcatttg tcattttaca cgacctccat caaatttatt ttgttgtcaa ttgttctttt
    16381 atttcttcat ctactcttat cactgtcttt ctattcgtcc tgtgtactac tagaaggcta
    16441 ataagcgcgc taatcaacag caagatactg ccattgacga aaacagcaag tgttttactc
    16501 ccaagagaag caaaagagag aaagagcaaa ccatatggtg caatgaaagt acctaaatta
    16561 gcgccaacca ataagattga agtattgaaa ctctccgcgt acttgggcgc ttggatactt
    16621 acttggttga ataagtaagg aatgaataac gaaaaagaaa ttcctgttag gatcgcacca
    16681 aacccaatca catacaggtt tatcacagtg gcaatcaata caaagccgat tcccatcagt
    16741 agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct gaagccagcc aaaacaaact
    16801 ccggcagcca tacttgccaa ttgcatgatt gttaagatat tcgctgcatt agtttcgttt
    16861 cctagagaat aagtcgtcag gactgtggct aatttaatcg atacagcatt atataccacg
    16921 atgaccagca agagaaaaat accataacta atcattttag gattcgtttt gattacttgt
    16981 cttgttgggg ctgtgccatg cgattgaagt tcagttgttt caggtgtagg aacaaacaaa
    17041 acaaacaaga caagaatagg taagctgatg gcgtagacta aaaaggtata ttgccaaccg
    17101 aactgtacta gctgtcctgc aacaaatgtc attaaagctg caccaaatcc ttgaaatgca
    17161 ctttgaaaac caatcataga ggctcgttca tctccgtgat aaaaggcagc aatcatgctg
    17221 actgccaaag aattgatcaa tccaaagcca atacctagtc cagctcttga agctaagaca
    17281 atactgtaac tttgtgagaa gagggggagg ataccacaaa tcgatgcaat cactaatccg
    17341 atgatcacag tttgtttttg tccgattttc ctagcaataa aactactaat caaaacaaaa
    17401 atagtgacca taaaagaggg agcagtcgtt aataattcaa cagcagataa agaacgcgtg
    17461 gcataagtag ctgacatact gggaattgca gacgtgactg caccagcaga agtcaaaata
    17521 agagagactg acagcagaga aaatttcatc atctttttat tcataagcat gttcctttca
    17581 tttttgtaag cgcttatcaa aaatatagta tgaagtggtt attctctcaa tagcggaatc
    17641 acaggaagtt catgtcccaa ccatagaaag tctttatgaa accaaaaata gctttgcata
    17701 taatagcagt acagcgtgaa ctaataagcg aaagaaaccg cttacaaaaa ggagggagtc
    17761 aattggaact agctgaatta gacatttttt ttgcgtaacg aagaatcaat cgaaactctg
    17821 cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag cttaccatct tcagacaccg
    17881 ccaaaaatcc accgagggga tctatttcaa cagggaaata ttcatatcag taaacaccgt
    17941 cgatatgcgg aagttcccga gcatacacat gattttgttg aaatcaatta tatgtatagc
    18001 ggtacatgtt tacaaattat taatggagaa aggatagaga tggaagaaag tgacgtggtg
    18061 atcattgata aagaagctac tcaaagaatc gaatacacag ggcaagagga tattttaatc
    18121 aacatcttat taaaagaaga aaccttatct acagagatat tgaatcattt ggcaagcaca
    18181 aataatctac tttcttcttt tttgataaat gcatcaaaag aaaaagggga acatacacaa
    18241 ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc atttgattga aaatatgctg
    18301 ttgatttatt ttgatacata cagtcataag atccgttcgt taaatctcta tacatcacta
    18361 ttactgatcg aattgactca tttattagaa caacaaagct tttcatcagc agatggatgc
    18421 gcagaaaatg agatgcttag agtattagat tatatcgata agcattttac ttcttgtacg
    18481 ttaaaagaaa cagcagctca ttttggctac aatccggtct atcttagtaa taaattgaaa
    18541 atgtattttg atgcttcctt tcaagaactg gttctaaaga aaaaattcga ctttgcgttg
    18601 gaactgattc aagaaacgag tttttcgttg gaagaaatcg ctaatcgtat cggatataaa
    18661 caaacgactt ctttattcaa attattcaag aagtattcaa catgtactcc caatgaatgg
    18721 agaaataaac attaagaaga aaatctttca ctaaagcata tgaaggaaaa ttgttttagg
    18781 tgcgattgct gtcttattat atcgttgcag cgaatttttt tggatttatt ttcagttcta
    18841 aacttttggt tgtattatca ggggatatag gtgtaaacat ttgtcattta tctgttatgc
    18901 gtattcagga attcgtttcc atcaaaaatt ataataacaa ctattataat taacatgttg
    18961 ataaaatgaa taaacaagac tatacttttc atgaaagtaa caaatagtta tgaaaaggag
    19021 ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc gtcggtacag gttttgttgg
    19081 aacaagtatc gcttatgcaa tgatcaacca aggaatctct aatgaattag tattgatcga
    19141 tgtgaatcaa gaaaaagcag aaggggaagc attggacctt ttagatggta tggcttgggg
    19201 cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa tgtaaagatg ctgatatcgt
    19261 tgttatcaca gctggtatca accaaaaacc tggtcaatct cgtttagatt tagttaaaac
    19321 aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg ggatcaggat tcgacggtat
    19381 tatcgttgtt gcttcaaacc cagtagatat tttgacatat atcgcttgga acgaatctgg
    19441 tctgccaaca tcacgtgtta tcggtactgg aacaacattg gatacaactc gtttccgtaa
    19501 agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc cacggctata tcttaggtga
    19561 acatggtgat tcagaagttg ctgcatggtc acatacaact gtcggtggta aaccagtgtt
    19621 tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa ttagatgtga ttgctgataa
    19681 agtccgtaat gctgcttatg agatcattga tcgtaaaaaa gcaacttact atggtattgg
    19741 tatgagtact gcacgtatcg taaaagctat cttgaataac gaacaagctg tattgcctgt
    19801 atctgcttat ttaactggtg aatacgacga aaaagatatc tttacaggtg tgccatcaat
    19861 cgttgacgaa aatggtgttc gtgaagttgt tgaattatca atcaacgaag aagaaaaagc
    19921 aatgttcagc aaatcaacaa gtgctttaag agaagtattg aacactgtat tataagaaac
    19981 aatgaatatg aactaaaaat cctaaaactg cttaacagct gaaatagctg ttaagcagtt
    20041 ttttattgat atctaatagg taagcaaata aacagaatgg ttaaaattaa tcggtccgaa
    20101 aatgtatccg tacatatgaa catatgaaat ttaatctgtt gattacaata agcgaatcgt
    20161 tatcaatgtt tatctgtggt taattaaaaa atccttccgc aacggcaaa
//
LOCUS       DADJYG010000039        19446 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.039, whole genome shotgun sequence.
ACCESSION   DADJYG010000039 DADJYG010000000
VERSION     DADJYG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.039"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..3654
                     /locus_tag="JZ382_001128"
     CDS             127..3654
                     /locus_tag="JZ382_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAY2427325.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="JZ382_001129"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="JZ382_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAY2427326.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="JZ382_001130"
     CDS             7442..7783
                     /locus_tag="JZ382_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2427327.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="JZ382_001131"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="JZ382_001131"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY2427328.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="JZ382_001132"
     CDS             9140..11560
                     /locus_tag="JZ382_001132"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY2427329.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="JZ382_001133"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="JZ382_001133"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY2427330.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="JZ382_001134"
     CDS             12994..13731
                     /locus_tag="JZ382_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY2427331.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="JZ382_001135"
     CDS             13745..14560
                     /locus_tag="JZ382_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAY2427332.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="JZ382_001136"
     CDS             14573..15220
                     /locus_tag="JZ382_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY2427333.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="JZ382_001137"
     CDS             15236..15892
                     /locus_tag="JZ382_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY2427334.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="JZ382_001138"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="JZ382_001138"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY2427335.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="JZ382_001139"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="JZ382_001139"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY2427336.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="JZ382_001140"
     CDS             18234..18752
                     /locus_tag="JZ382_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAY2427337.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="JZ382_001141"
     CDS             18859..19359
                     /locus_tag="JZ382_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAY2427338.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6538 a         3340 c         4176 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggaca
//
LOCUS       DADJYG010000040        19316 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.040, whole genome shotgun sequence.
ACCESSION   DADJYG010000040 DADJYG010000000
VERSION     DADJYG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.040"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..455)
                     /locus_tag="JZ382_001142"
     CDS             complement(<1..455)
                     /locus_tag="JZ382_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAY2427339.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="JZ382_001143"
     CDS             complement(560..1423)
                     /locus_tag="JZ382_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY2427340.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="JZ382_001144"
     CDS             complement(1619..3718)
                     /locus_tag="JZ382_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAY2427341.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="JZ382_001145"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="JZ382_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY2427342.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="JZ382_001146"
     CDS             complement(5071..6912)
                     /locus_tag="JZ382_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY2427343.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="JZ382_001147"
     CDS             complement(7094..9487)
                     /locus_tag="JZ382_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAY2427344.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="JZ382_001148"
     CDS             complement(9594..10454)
                     /locus_tag="JZ382_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAY2427345.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="JZ382_001149"
     CDS             complement(10513..11034)
                     /locus_tag="JZ382_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAY2427346.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="JZ382_001150"
     CDS             11354..12073
                     /locus_tag="JZ382_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2427347.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="JZ382_001151"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="JZ382_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAY2427348.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="JZ382_001152"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="JZ382_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY2427349.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="JZ382_001153"
     CDS             16433..16789
                     /locus_tag="JZ382_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAY2427350.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17030..18034)
                     /locus_tag="JZ382_001154"
     CDS             complement(17030..18034)
                     /locus_tag="JZ382_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAY2427351.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18200..19051
                     /locus_tag="JZ382_001155"
     CDS             18200..19051
                     /locus_tag="JZ382_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY2427352.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19068..19313
                     /locus_tag="JZ382_001156"
     CDS             19068..19313
                     /locus_tag="JZ382_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427353.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5386 a         3991 c         3377 g         6562 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    16861 gagttgcttc tgcttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
    16921 cttttgtctc acgctctttt tttgttatat tcgtcttttc cgatccttct cttgacacaa
    16981 ttcatttaac tgatcactgg tacttgaaca cctaatattc tagtcatttt tataaacgat
    17041 tcaatacttc atccacattt ttgatcatca cggatatcgt accatccgga ttattcacaa
    17101 attcaatcaa atctgggttg cgatatacat ctactggtac gatcagttcg atcccgtttg
    17161 atagcttgaa tttttgtttt ccaaattttt tctcagaaat ttcttgtact tcttttacag
    17221 gaggtgcttc tgggacaaat ccttgctctt gtacttcttc tttaaaagcc atttttgccg
    17281 tgatgttttc cttgaagact ttttccgcaa tcatctcatg atccaaccga cctttttctt
    17341 ctatcgtatc atagacagct tctttcaaat cagccatgat atcaaattct tctgtctcaa
    17401 atttttttcc aagatgcttg gctacttttt ggatcacttt tacattttct tctaaagaag
    17461 gtgcaggaac actttcgatc actcttccag aaaaataaag ctcttttttg ccagaaaact
    17521 catatttttt ttcaattaat tcgtaggtcc aatcagtcaa attcacagcg aatgcttcat
    17581 ctgctttttg tgttttcgcc cctagaattg ctcgattcaa aatcaagcgg ttgtcgatcc
    17641 ctgcttcgtc tgcttcaaca aagtgagtgt aagcctcacg ataattgact ttcaataaag
    17701 ataagtacat cactgtatct aattcataca agacaacaaa tacatctgca ctaggtgctt
    17761 cttcactttc ttggtagata tcgaaccacc gctgaacgat tgcttcactt gcttccacaa
    17821 aattttcttg agcgatttta agtgattcaa cgatcatgct ttcttctgct agttgtccag
    17881 tttttgtttg agcagaagag attttttgga tcttcttttg aagaaaatca cgaatatatt
    17941 cttttgtcag atccagctct ttttgtgaaa agactggaga accagtttct ctgtcaataa
    18001 tatgcagaat tgcttttttt aaatagatat ccattttctc attcccttct gtcttagttt
    18061 acggaaaaaa aatcaaaaat accagaatca aaatcatttt cttttaaaca aaaatttcaa
    18121 gagtagaaac aaaatggatt ctttttttcg aaaaatgcgc tacacttata gttgaacgaa
    18181 taaaaaagga gctgaaagta tgaattcatt aacaagcact tatcgtctat caaatggtta
    18241 cgaaatcccg gtagtaggat tcggcacatg gcaaacacct gatggggatg tcgctgtttc
    18301 ctctgttaaa gaagcgctgg ctgcaggtta tcgccatatc gatactgccc aaggatacaa
    18361 gaatgaagaa agcgtcggcc aagcgattaa ggaaagtgga attcctagag aagagatatt
    18421 cttgacaaca aaattatgga atgcaaacca cagctatgaa cttgtgatgt cttcttttga
    18481 agaatcattg aagaagctcc aaacagatta tttagatctg tttttgatcc actggccaaa
    18541 ccctgttgct tttcgtgata attgggaaca agcaaatgca gatacatggc gagcatttga
    18601 agaactgtat gaagcaggta aaatcaaagc aatcggtgtg agcaatttct taccccacca
    18661 tctagataca ctagcaaaaa cagcaaaaat tatgccgatg gtcaatcaag ttttccttgc
    18721 accaggcgaa ttacagacaa ctgtagttga gtatgcaaaa aaacatgata tgatcctcga
    18781 agcatatagt ccattaggca ctggtaaaat cttcgatgtg cctgaaatga aacaaatcgc
    18841 tgaagcacat gacaagacga ttgcacaagt tgctttgcgt tggtcgctgc aacacgaatt
    18901 ccttcctctt cctaagtcag tgacaccaag cagaatcaaa gaaaatactg aattatttga
    18961 ttttgaatta acagaagaag aaatgaaaca aatcgatcag ttagatggtg tagtcggcaa
    19021 agcaaaagac ccagatacta cccaatttta agtcgaaagg tgattttatg gatccaatcg
    19081 aacgcttgaa ttctctctcg gaagaagtta cccagacttt tcattccgat tttgtctttt
    19141 tgatcgatgc agagaaaatc caacattttc ctgcaagaaa ctggacacat gatcaaatca
    19201 tcgaagaact gaaaaaacgg ttcgatcatt cgctaatggt caaaccctgg cacgaacatg
    19261 aagtcattta ttcacctgaa ctacctgttt ttgccttgat accgaaaaaa tagaaa
//
LOCUS       DADJYG010000041        18945 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.041, whole genome shotgun sequence.
ACCESSION   DADJYG010000041 DADJYG010000000
VERSION     DADJYG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.041"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..538)
                     /locus_tag="JZ382_001157"
     CDS             complement(17..538)
                     /locus_tag="JZ382_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAY2427354.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="JZ382_001158"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="JZ382_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY2427355.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="JZ382_001159"
     CDS             4618..5505
                     /locus_tag="JZ382_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAY2427356.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="JZ382_001160"
     CDS             5502..6671
                     /locus_tag="JZ382_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427357.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="JZ382_001161"
     CDS             6695..8050
                     /locus_tag="JZ382_001161"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY2427358.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="JZ382_001162"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="JZ382_001162"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY2427359.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="JZ382_001163"
     CDS             10336..10626
                     /locus_tag="JZ382_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427360.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="JZ382_001164"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="JZ382_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY2427361.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="JZ382_001165"
     CDS             11667..12326
                     /locus_tag="JZ382_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAY2427362.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="JZ382_001166"
     CDS             12483..13415
                     /locus_tag="JZ382_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427363.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="JZ382_001167"
     CDS             13569..14858
                     /locus_tag="JZ382_001167"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY2427364.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="JZ382_001168"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="JZ382_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAY2427365.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="JZ382_001169"
     CDS             16438..17025
                     /locus_tag="JZ382_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY2427366.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            17390..18718
                     /locus_tag="JZ382_001170"
     CDS             17390..18718
                     /locus_tag="JZ382_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427367.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18729..18938
                     /locus_tag="JZ382_001171"
     CDS             18729..18938
                     /locus_tag="JZ382_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427368.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6201 a         3374 c         4079 g         5291 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    18901 atatgaaaaa aacaagaagc gaaaataaaa aacgataaaa cggct
//
LOCUS       DADJYG010000042        18740 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.042, whole genome shotgun sequence.
ACCESSION   DADJYG010000042 DADJYG010000000
VERSION     DADJYG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.042"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            256..531
                     /locus_tag="JZ382_001172"
     CDS             256..531
                     /locus_tag="JZ382_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427369.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="JZ382_001173"
     CDS             544..2616
                     /locus_tag="JZ382_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAY2427370.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="JZ382_001174"
     CDS             2781..4667
                     /locus_tag="JZ382_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAY2427371.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="JZ382_001175"
     CDS             4679..5626
                     /locus_tag="JZ382_001175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY2427372.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="JZ382_001176"
     CDS             5646..6173
                     /locus_tag="JZ382_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAY2427373.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="JZ382_001177"
     CDS             complement(6236..6889)
                     /locus_tag="JZ382_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY2427374.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="JZ382_001178"
     CDS             7021..7863
                     /locus_tag="JZ382_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAY2427375.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="JZ382_001179"
     CDS             8021..8917
                     /locus_tag="JZ382_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAY2427376.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="JZ382_001180"
     CDS             8920..9633
                     /locus_tag="JZ382_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAY2427377.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="JZ382_001181"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="JZ382_001181"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY2427378.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="JZ382_001182"
     CDS             10168..10395
                     /locus_tag="JZ382_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAY2427379.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="JZ382_001183"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="JZ382_001183"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY2427380.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="JZ382_001184"
     CDS             complement(11181..11723)
                     /locus_tag="JZ382_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAY2427381.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="JZ382_001185"
     CDS             complement(11960..12358)
                     /locus_tag="JZ382_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAY2427382.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="JZ382_001186"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="JZ382_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAY2427383.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="JZ382_001187"
     CDS             13471..14238
                     /locus_tag="JZ382_001187"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY2427384.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="JZ382_001188"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="JZ382_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY2427385.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="JZ382_001189"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="JZ382_001189"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY2427386.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="JZ382_001190"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="JZ382_001190"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAY2427387.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DADJYG010000043        18716 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.043, whole genome shotgun sequence.
ACCESSION   DADJYG010000043 DADJYG010000000
VERSION     DADJYG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.043"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..1452
                     /locus_tag="JZ382_001191"
     CDS             25..1452
                     /locus_tag="JZ382_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY2427388.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1544..1909)
                     /locus_tag="JZ382_001192"
     CDS             complement(1544..1909)
                     /locus_tag="JZ382_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAY2427389.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1906..2271)
                     /locus_tag="JZ382_001193"
     CDS             complement(1906..2271)
                     /locus_tag="JZ382_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAY2427390.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2463..2711)
                     /locus_tag="JZ382_001194"
     CDS             complement(2463..2711)
                     /locus_tag="JZ382_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427391.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2710..2838
                     /locus_tag="JZ382_001195"
     CDS             2710..2838
                     /locus_tag="JZ382_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAY2427392.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2895..4589
                     /locus_tag="JZ382_001196"
     CDS             2895..4589
                     /locus_tag="JZ382_001196"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAY2427393.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4656..4862
                     /locus_tag="JZ382_001197"
     CDS             4656..4862
                     /locus_tag="JZ382_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427394.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5240..6373
                     /locus_tag="JZ382_001198"
     CDS             5240..6373
                     /locus_tag="JZ382_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY2427395.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6404..6724
                     /locus_tag="JZ382_001199"
     CDS             6404..6724
                     /locus_tag="JZ382_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427396.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6753..7217)
                     /locus_tag="JZ382_001200"
     CDS             complement(6753..7217)
                     /locus_tag="JZ382_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427397.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7349..8602)
                     /locus_tag="JZ382_001201"
     CDS             complement(7349..8602)
                     /locus_tag="JZ382_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2427398.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8631..8948)
                     /locus_tag="JZ382_001202"
     CDS             complement(8631..8948)
                     /locus_tag="JZ382_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAY2427399.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8974..9294)
                     /locus_tag="JZ382_001203"
     CDS             complement(8974..9294)
                     /locus_tag="JZ382_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2427400.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9758..11173)
                     /locus_tag="JZ382_001204"
     CDS             complement(9758..11173)
                     /locus_tag="JZ382_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2427401.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11190..12506)
                     /locus_tag="JZ382_001205"
     CDS             complement(11190..12506)
                     /locus_tag="JZ382_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2427402.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12656..13354)
                     /locus_tag="JZ382_001206"
     CDS             complement(12656..13354)
                     /locus_tag="JZ382_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY2427403.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13643..14632)
                     /locus_tag="JZ382_001207"
     CDS             complement(13643..14632)
                     /locus_tag="JZ382_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY2427404.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14712..15545)
                     /gene="msrA"
                     /locus_tag="JZ382_001208"
     CDS             complement(14712..15545)
                     /gene="msrA"
                     /locus_tag="JZ382_001208"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY2427405.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15720..17354)
                     /locus_tag="JZ382_001209"
     CDS             complement(15720..17354)
                     /locus_tag="JZ382_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427406.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17341..18714)
                     /locus_tag="JZ382_001210"
     CDS             complement(17341..18714)
                     /locus_tag="JZ382_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAY2427407.1"
                     /translation="MISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAI
                     ALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFL
                     SKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSIS
                     DSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPW
                     FLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTS
                     IAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKV
                     GESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVN
                     VDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEI
                     NKKNENK"
BASE COUNT         5913 a         3693 c         3133 g         5977 t
ORIGIN      
        1 aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa tattttaaga
       61 cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc
      121 aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat aaaaaaagaa
      181 tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca
      241 gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa
      301 acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa
      361 ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta ctttaatgac
      421 tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg
      481 cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt
      541 tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat
      601 aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg ccagcaagtg
      661 tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat
      721 tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc
      781 gcctttattt tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt
      841 gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa
      901 ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca accgttcgaa
      961 cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa
     1021 aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca gacaattttt
     1081 tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct
     1141 tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc
     1201 gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact
     1261 ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg
     1321 cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa
     1381 tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg
     1441 caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc
     1501 ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta ttttggacat
     1561 ctaggggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg
     1621 ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg
     1681 aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt
     1741 tctcgcttac tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct
     1801 tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta
     1861 caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc
     1921 tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg
     1981 aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc
     2041 gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt
     2101 tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc
     2161 aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt
     2221 taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac
     2281 ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct
     2341 atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccatt
     2401 tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag
     2461 gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa
     2521 tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac cctattttta
     2581 tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct
     2641 atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag
     2701 gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt
     2761 ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat
     2821 gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca
     2881 ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata
     2941 tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt
     3001 attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat
     3061 attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca
     3121 tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta
     3181 ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct
     3241 ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat
     3301 tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag
     3361 aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa
     3421 caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag
     3481 aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg
     3541 gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact
     3601 tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag
     3661 aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat acactgactc
     3721 aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca
     3781 ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg
     3841 gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg
     3901 acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa
     3961 accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga
     4021 attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta
     4081 ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg
     4141 gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga
     4201 atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag
     4261 tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct
     4321 atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca
     4381 ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga
     4441 tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg
     4501 gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag
     4561 ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc
     4621 aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat
     4681 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gctgccctgc
     4741 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4801 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     4861 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     4921 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     4981 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5041 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5101 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5161 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5221 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5281 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5341 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5401 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5461 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5521 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5581 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5641 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5701 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5761 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     5821 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     5881 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     5941 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6001 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6061 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6121 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6181 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6241 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6301 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6361 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6421 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6481 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6541 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6601 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6661 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6721 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6781 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     6841 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     6901 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     6961 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7021 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7081 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7141 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7201 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7261 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa aacggaaaac
     7321 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7381 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7441 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7501 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7561 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     7621 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     7681 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     7741 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     7801 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
     7861 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     7921 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     7981 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     8041 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     8101 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     8161 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     8221 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     8281 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
     8341 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
     8401 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
     8461 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
     8521 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
     8581 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
     8641 aatcgacgat acagattaat gatctctttt gctaaatctg taaaagctat agatgtcatc
     8701 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
     8761 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
     8821 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
     8881 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
     8941 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
     9001 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
     9061 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
     9121 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
     9181 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
     9241 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
     9301 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
     9361 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
     9421 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
     9481 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
     9541 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
     9601 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
     9661 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
     9721 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
     9781 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
     9841 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
     9901 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
     9961 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    10021 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    10081 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    10141 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    10201 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    10261 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    10321 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    10381 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    10441 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    10501 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    10561 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    10621 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    10681 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    10741 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    10801 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    10861 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    10921 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    10981 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    11041 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    11101 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    11161 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    11221 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    11281 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    11341 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    11401 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    11461 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    11521 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    11581 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    11641 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    11701 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    11761 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    11821 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    11881 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    11941 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    12001 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    12061 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    12121 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    12181 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    12241 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    12301 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    12361 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    12421 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    12481 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    12541 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    12601 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    12661 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    12721 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    12781 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    12841 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    12901 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    12961 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    13021 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    13081 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    13141 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    13201 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    13261 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    13321 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    13381 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    13441 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    13501 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat atgatgttca
    13561 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    13621 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    13681 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    13741 ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg gccatttgcg
    13801 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    13861 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    13921 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    13981 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    14041 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    14101 tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat gcttttcctt
    14161 gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca gggtctgggc
    14221 gatgatgata gaagatgtca acataatcta gccccattct ttggagactt tggtcacaac
    14281 ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac atatgataac
    14341 ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct ctaaaaatac
    14401 gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca aggtcgaaat
    14461 gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg ataggatcat
    14521 aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa cctgaatgac
    14581 cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac aatttttctc
    14641 ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta ctacatttta
    14701 aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt gttttttact
    14761 cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg tttcggttgt
    14821 tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc tggttcgatt
    14881 tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt tgattgttcc
    14941 gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg cttccctcta
    15001 tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat tgtcagcaac
    15061 tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac atgacctgtg
    15121 taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt gtatcctgac
    15181 aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca gaaacatccc
    15241 cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt ggaaaagtca
    15301 tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat tacatcaggc
    15361 gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga taactgttct
    15421 ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt tcgttcccaa
    15481 tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat ttgttcctca
    15541 tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg taatttatca
    15601 taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat aaaaaagaga
    15661 gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga aaagttaatt
    15721 taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa tgtaacaaga
    15781 taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc aactcctttg
    15841 actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg ttgatgattt
    15901 atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg gctcttctca
    15961 ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt tacccattga
    16021 ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga agcgatagga
    16081 gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac tgtttctttg
    16141 tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt tttactagaa
    16201 acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc tcttgtctta
    16261 ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa tattatttgc
    16321 aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg cagaagatac
    16381 catggcacca aactagttgt agctagccaa gtcaaacacg taccataata aatctttttt
    16441 tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa cagaccaact
    16501 agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact atcgaacaca
    16561 ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg gtagagcatt
    16621 tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc tgccgcacta
    16681 aataattttt taaatatagc aataaaatct gcattcttga aacctttaga aaccaaacga
    16741 ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac atggctgtac
    16801 actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc ttttccagag
    16861 atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat agcttcggca
    16921 attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata gagacaacta
    16981 aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata agctacgagt
    17041 gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa attttttgtc
    17101 atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata atttacacca
    17161 tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt cgataacccc
    17221 ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg tgtatttttg
    17281 aaaaagatga aagaatatat actactaact ataattaatc cactcaatat tactactttt
    17341 ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag aatctttcaa
    17401 cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag tcagaatact
    17461 gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta tcttattttc
    17521 tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat ttacgacttt
    17581 accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca ctttttgctt
    17641 tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact tccctttttc
    17701 ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt ttatcgcttc
    17761 tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg ggagtgtttc
    17821 ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat tttcttttgc
    17881 tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc atgaatattt
    17941 tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa aaaagatttg
    18001 gatacctaag atacgccaag gaaactggag taagcttatc ggtgtttttt gaaacaaaaa
    18061 ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat agatgatttt
    18121 ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa ccaatcctaa
    18181 taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac tatctgaaat
    18241 agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt gataattcat
    18301 ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa aaacagcctt
    18361 tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta aaaacaaacg
    18421 aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga catgagtata
    18481 cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat tgaatatcaa
    18541 tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt ctgcaattgc
    18601 ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac aactaaataa
    18661 aaaagcagca tctatctttt taaaaaactt ttctccataa taaaaactaa tcagaa
//
LOCUS       DADJYG010000044        18566 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.044, whole genome shotgun sequence.
ACCESSION   DADJYG010000044 DADJYG010000000
VERSION     DADJYG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.044"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            136..558
                     /gene="mgsA"
                     /locus_tag="JZ382_001211"
     CDS             136..558
                     /gene="mgsA"
                     /locus_tag="JZ382_001211"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY2427408.1"
                     /translation="MKIALIAHDRKKSMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKRPLSF"
     gene            881..1417
                     /locus_tag="JZ382_001212"
     CDS             881..1417
                     /locus_tag="JZ382_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAY2427409.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSTFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1457..3220)
                     /locus_tag="JZ382_001213"
     CDS             complement(1457..3220)
                     /locus_tag="JZ382_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427410.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVLLGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVATLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIILLSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGEISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYQKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEETPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITIDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3223..4938)
                     /locus_tag="JZ382_001214"
     CDS             complement(3223..4938)
                     /locus_tag="JZ382_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427411.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIRKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEISTTPDFSSESILA
                     FDHVDFRYTPDSGLSLQGITFQLKRNTVLGIIGPTGSGKTTLTQLVPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGSIRENLQWGKQDATEDECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPELLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            5608..6162
                     /locus_tag="JZ382_001215"
     CDS             5608..6162
                     /locus_tag="JZ382_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427412.1"
                     /translation="MFAVEVDASVIDISNEKKEINISPVLTFDEMVREVAKENGIPII
                     QAQQELGFTDESARQARTARATYRTLSQSFTVNASYRPTMRFYCETSESGNFRAIKRI
                     LRVEMIRGYNGLSKQFGGTVYVHLEDANRIFYIVNGDFFNNGSTTWNAGVNIGVGRNA
                     SIKFGVTNTTSHYQYRYVESRLRF"
     gene            complement(6412..6636)
                     /locus_tag="JZ382_001216"
     CDS             complement(6412..6636)
                     /locus_tag="JZ382_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427413.1"
                     /translation="MKSDKSKKRKYTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IDVDTQYIAETGFLFIFILIFALYKSFHTK"
     gene            6839..9607
                     /locus_tag="JZ382_001217"
     CDS             6839..9607
                     /locus_tag="JZ382_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2427414.1"
                     /translation="MLKNEILCYLENQTAFFDLNHMNEVFTAQRLAEHFGVKRNTVSH
                     YLNQLNEEKLLVKINSRPVVYFHKEAFEKQFFQLRTNFYFSINDLKEEQPFFAQPKDL
                     FSFMIGHDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVHLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNVSRRIKTRLFFATTEETTSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELVYSFFIEEQRKMGSPLSVSGPVLSLLAGQNLSGNIGELKNI
                     VKVSAAKAYAEQREQSEIHVTIHHLQKELLAEPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILLDFKKNECLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMESAYQIKFDGNSVYALSYYLYQRSTVKWLPEEHAIIQLIKELEKQV
                     SQSYPSSYHYVQRILELSKIKLDIEVTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEIVDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNAALEEIAEEAVRQTKPEWRL
                     LYPQENKPKALITTCFTGIGTAAHLSALLEKSLPTTCQLKIIPYEYQRLKDKKNRDPL
                     FSIYEIVGIIGTADPAITDIDYLSLEDLISGEKMSVLAEWLDSAMNVSDKETFNQNII
                     RNFSLEKVLDSVTILDTEKVMEEIDHFMRELEVQLNRRISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWEQCQKEEIFAIKSAFSVIEEHYSVMIPRSEIAYICDILSESTE
                     LLSIEEEF"
     gene            9688..10107
                     /locus_tag="JZ382_001218"
     CDS             9688..10107
                     /locus_tag="JZ382_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAY2427415.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITALDCYTDENFDL
                     TESVSQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINQDHVYLVAGLNLPLLIELVSRL
                     DAEVQTTVLIQQVLTDSKKMIQFCNERINQEIEEDEF"
     gene            10119..10589
                     /locus_tag="JZ382_001219"
     CDS             10119..10589
                     /locus_tag="JZ382_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2427416.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            10606..11385
                     /locus_tag="JZ382_001220"
     CDS             10606..11385
                     /locus_tag="JZ382_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2427417.1"
                     /translation="MIVQAVLLGIVAFIAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADSYAEEGNYKGVEHMHLLAGFGLSLMLGVVVMVSYMVG
                     SNTIGNLLNKIPDFVQNGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLK
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTVGEVEEDEEF"
     gene            11372..12193
                     /locus_tag="JZ382_001221"
     CDS             11372..12193
                     /locus_tag="JZ382_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2427418.1"
                     /translation="MKNSESKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGVVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            12210..13259
                     /locus_tag="JZ382_001222"
     CDS             12210..13259
                     /locus_tag="JZ382_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY2427419.1"
                     /translation="MVTMQDYIYEEKEVLSTILKKNDFSTRKQMKKTTNLLMLATGSS
                     YNACLAAKPALESYGELTVDIQEPFHFAHYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQEIPIIAVTNDVQSPLALEATQIIDLGAGIETVGFVTKGYSATVLQLLLLGLGIG
                     ISKNNISKEIEQNYVQQLHKIINHLPVIIQKTEEFFDEHQSLFRLTQRFITIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGDNENKERSQKLRR
                     YMSQYVGETLTITTKKARDEKTLGLAIECNEYLSILALVVPFQLFAYKIAAAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            13272..14276
                     /locus_tag="JZ382_001223"
     CDS             13272..14276
                     /locus_tag="JZ382_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY2427420.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEKIIDKLYTKKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVVVSSVTGTTQEVVKA
                     VEEIKKVGATLIGFIDKAESKLSQLCDFVVTYPAPGTEQIKFFMVADRLMYLHGEFED
                     YSEYYEQLEHYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHAVTQRIDAHVEQMNCHPLEIRRYYRQFDY
                     "
     gene            complement(14340..14984)
                     /locus_tag="JZ382_001224"
     CDS             complement(14340..14984)
                     /locus_tag="JZ382_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAY2427421.1"
                     /translation="MKKSTLLGLGMLIATTGIAALSSVDVSAHGYVSQPISRGYQGAL
                     DSRNNWQSAFEKYGRIINEPQSLEAPKGFPSAGPANGRIASANGAVGDFVLDKQTATL
                     WTKNNLTTGANRFTWHFTADHATTKWHYYMTKNGWNPNAPLNKNDLEFIGEVNNRGNM
                     SNTNPTHTINIPSNRLGYHVILAVWDVADTDNAFYNVIDVNVQNRNALTDLENN"
     gene            complement(15116..16000)
                     /locus_tag="JZ382_001225"
     CDS             complement(15116..16000)
                     /locus_tag="JZ382_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2427422.1"
                     /translation="MIKHASLIRKLRKERGLTQAQLTEGLSQRGTLAAFESRGTKISF
                     ELLVDYLERMNVTLEEYQFLLNNNTLTGKQKLSNYLITTKYLTKEQENELLKEYKKTG
                     NIYYRLLYTQRILIDQYLGNRKLTRSETLTEVSIIKKYLEGIDTWGHYELSIFSNCLF
                     IFDDEYIYHSFQRSVTKMKAYVDTAHYSELLSNFLLNGIHLSFHRSSVFLRKLFLGEW
                     KKVSDRYKHTLDLARYKAFTALALLADGNERALEEIDEAIHFFEWLGLEEVKEEIIEL
                     RDSLLKKTIHKNDKAIYQ"
     gene            16207..17943
                     /locus_tag="JZ382_001226"
     CDS             16207..17943
                     /locus_tag="JZ382_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HAY2427423.1"
                     /translation="MKKILWLSLAAIGCFAGGGQTIYAAQDSAALMPDISEKQIAVGY
                     YHNWVPEQGAGYRGGKPAETDLGKINPFYNVIAVSFMKGEGIPTFEPFNMSDEEFRSK
                     VAALNEEGRSVIISLGGADSHIELYRGQEQAFADEVIRLVETYGFDGLDIDLEQSAIT
                     AGDNQQVIPEALKIVRDHYKKQGKHFIISMAPEFPYLRTAGGYVPYITALENEYDFIA
                     PQLYNQAGDGLSVGDEWIAQNNDSRKYDFLYGMSKALHNGESGYIRIPADRLALGIPA
                     NNDAAANGYVQDPSVVYQVFEQMEKERTPLKGLMTWSVNWDEGVNSAGISYNESFAKA
                     YQNLFKEQEPDTEKPSKPTNLRGTATSTTVTLSWTESTDNVRVSHYNIYENNRLVGTS
                     NTTNYIHTGLQPETTYSYTIEAVDTSGNISARSDDLNIRTQEAAVLPAPSIPNGLSIN
                     HVTQSTVRLVWQENSLLEEVLYYEIYRDGKKIATSQTAVFEDQNLTASTAYRYQVRAV
                     NQTGASLLSQSVTARTLDENGQGNSWTVGTFYQVGDIVTYNGIQYRCVQAHIAAAHWA
                     PDTAFSLWQKIS"
BASE COUNT         6021 a         3295 c         3781 g         5469 t
ORIGIN      
        1 tgtcatgttg aacaagaatt ttttcttttt tttgtcaatg tgctagtttt aagcttcaat
       61 aaatatccat aagaaataaa aactgataca ctggagtaaa taagtatgtt aaccatatca
      121 aaaggagtgt ctcttatgaa aatcgctttg atcgctcatg accgaaaaaa atctatgatg
      181 gtgaaattag caacagcgta taaaacaata ttggaaaaac atgaactttt tgctaccggg
      241 acgactggac aacgtatcac ggaagcgacc ggcttgccca ttcatcggtt taaatccgga
      301 ccgttaggcg gagatcagca aattggagca atgatctcgg aggaccagtt agatttggtg
      361 atttttcttc gagacccgtt ggctgcacag ccgcatgaac cagatgtaac agcacttatc
      421 cgtttatctg acgtttatga gattccgttg gctacgaata tcggaacagc tgaggtgctg
      481 ctaagaggat tggaagccgg cttcctagat tttcgaagaa tcgtccatga aatagacaaa
      541 cgtccacttt cattttgatg gacaagtgta tggaaaaata acgtttaatg ttcgtctata
      601 tgtagctata aaagctaaac aaactgaaaa tatgcaatat aatacgaacg tattgtataa
      661 tataaccaat aatattagtt tttcgtgttt gcagtaaatg tgaaaatatg ctatagtttc
      721 tgtgggaaaa gatcccaaaa ataatacaga gtagaaaata aagcgttaag tgctaagagg
      781 atgggatgtt gcctcttgga cgaaggacgt aatgttcgcg gttttatttt cgcattcgct
      841 gcatgatacg atgtagcagc tcttaaagga gatgctaaaa atgaagaaac accggttaga
      901 cgcacgtatg attgctatta tgggcttact gattgcattg atggtcacgt tgtcccgtct
      961 ggtagcaatc gagaccccat ttattaaaat cagtgtcaca tttattccac aagtgatcat
     1021 gggcattctt tttggtcctt tttggtcagg gataggcgca gttttagctg atttagtagg
     1081 aatggctctc ttctcaaaat ctacattttt tattggattt acattgaatg catttattga
     1141 aggagcaatc tacggattct ttttctatcg aaaagaaatc acttggaaaa atgcgatttt
     1201 ggctacgctt agtgtcacgc tgatcataaa cttatttttg acaccattat ggcttgcgtt
     1261 gatgtaccat gtgccattgt tcagctgggt cgtttgggcg ccccgcctgt taaaaacagt
     1321 gatatggtta ccgattcaat caattgccat ttattacgta ggacgtagta ttccttacaa
     1381 aaaaatttta agaagtttag cgatccacgc taaataaaaa acaagcagtc ttcccatccc
     1441 agaagactgc ttgtttttat tgtgctgtga attgtgcttg atagagccgt cggtaataac
     1501 catctttttg aagcaactca tcatgggtgc ccatttcgac aatggctccc tgatccatca
     1561 ctaatatctg gtccgcattt ttgattgtgg acaggcgatg ggcaataaca aaactggttt
     1621 taccttccat cattgctaag aaggcttgtt ggattttttt ctctgtcaat gtatcgactg
     1681 agctagtagc ctcatctaag atcagcatag gcggattact gatcatcgtt cgagcaatcg
     1741 tcaacagttg gcgttgtcca tctgatatct ttaacccttg accgccaatc ttagtatcta
     1801 atttttccgg caaacgttca acaaattcat acatataaga agcttttagg gcatcattga
     1861 tttctgcgtc acttgcttcc ggattcccaa aacgaagatt ttctcgcaat gtactgtcaa
     1921 atagccaagt atcttgtaac accattccaa aacttttacg taatgtatca cgttggatag
     1981 agcggatgtc atggtcatcg attgtaatcg ttccttttgt tgtgtcataa aaacgcatca
     2041 gcaaattaac aagtgtggat tttccggctc ctgtttttcc tactatagca atggtttgac
     2101 ctggataagc agtaaaattg aaatcttcga tcaaacgctg tttggggtca taagaaaaag
     2161 aaacatgatc aaaagagact tttcctttga tatttttggg ttccaaggca taggcatctg
     2221 gcaaatcagg cgtttcttct ggttgatcga tcaaatcaaa tgctcgttct agaccagaaa
     2281 aagcggtttg cagttgtgtc gtgattccag aaagttcgat aaatggttta gaaaactgac
     2341 ttgagtagat cgtaaagctg gaaatcacac caacagtgat tgacgaacca ctcttcaatg
     2401 ccaataggcc gccaaccaaa ccaatcgata aataagcaag gtgatctaca aaacgagaag
     2461 acgggtttgt caaggaagaa gaaaattgtg cccgttgtcc tttttgatat agatcagcat
     2521 ttaacagttc aaattgctgt tgactagctt cttcttgctg aaaagctttc acgattttct
     2581 gattaccgat tcgttcggtc acaaagcccg aaatctcccc aatgattctt tgctgtctat
     2641 cgaaatttgt ttgcgaagca cgagcaacga tccaactcac aaggaaaata atcggtgtgg
     2701 aacaaagaac gaccaatgtc agaagtggac ttagacgcat cataaaaata aacgaaagaa
     2761 ggatgatcgc aactccggaa aacaattgat tgaaggcggc agaaatcgca atagaaatgt
     2821 tgtccatatc atttgtaaat cgactgacga tatttccatg agaattttga tcataataac
     2881 gaagtggcaa tcgatttaaa tgggcaaaag catcttttct taatgttgca acggacaagt
     2941 aagctaaccg attgcttaat acttggatga gccattgact gactacattg atcaaaacga
     3001 ttccaccgaa cagaatcagt aaatggaata gtcgattgaa gcgaacttgt ccttctccga
     3061 tcatttgatc gatactttgg ccaatttgta acgtcatcac aactgttgcg atcccgttga
     3121 taatccctag taatacagct agccaaatgt ctttcgcata agcagtaaga taagggatga
     3181 agcgacggag tgtctgcaag gataattttt ttactttcat aactattctt cctcctgtga
     3241 tgcgacgatt tcctgatatt ccgctgattg agcaagtaat tcttcatgcg taccttgagc
     3301 agcgatcttt ccttgatcca tcactaagat atggtgtgct tcttgaatgg agcggacacg
     3361 ttgggagata atgatcactg tactgccaag ataagtctgc agcgccttac gcaaatccaa
     3421 gtcagtctga taatccaacg cactcaaaga gtcatctaaa atcagtagtt ctggcttggc
     3481 aatcaaagct cttgcaatcg ttagtcgctg tttttgaccg ccggagaaat tttttccgtt
     3541 ttccatgact tttgtatcta aaccatttgg aagctgacgt acaaattctt ctgcctgagc
     3601 gattttcagg gcttcccaac actcatcttc tgtggcatct tgcttacccc attgaaggtt
     3661 ttcacgaata gacccagtaa ataacacgga agtttgggga acaagggcga tttgctgacg
     3721 aagatgatcc agctgccaag atcgaacgtc cattccatcc attatgactg taccggaact
     3781 gacatcatag aagcgcggaa ccaattgagt caaagtcgtt ttcccgcttc ccgttggacc
     3841 aatgattcca aggaccgtat ttcgcttcag ctgaaatgta atcccttgaa gagaaagccc
     3901 agaatcagga gtataacgga agtcaacgtg atcaaaagcg agaatcgatt cagaagaaaa
     3961 atcgggagta gtactgatct cggcttcaag aatcgaattc tctgtgtcta gaacctcttt
     4021 tactcgatta cctgaagctt cggcacgtgt gaagatcacg acgaggtttg aaacaacgat
     4081 cagtgccaaa agcatttgat tcatataatt gatcaaagca agaacttgtc cttgttgcaa
     4141 actaccaatg tttactttga tcccgccaac tgttagcaaa caaatgattc cgacattcat
     4201 gatcaaggtc gtagcaggtg ataaaagggc agagatattg gaaacacgta aataattgtt
     4261 cgctagatca tttgtgcttt tatcaaactt ttctgtttct ttttttgtct gtgcaaatgc
     4321 acgtatgaca cgaacgccgc taagattctg gctgacgtgt tcattgagct ggtctagttt
     4381 ttcttgcact ttttgataga gaggaacagt ttttctgatg ataaaaaata aaatgatgga
     4441 gaaaataggc aataatgcta agaaaaacag acctacttcc cagtcgatca caaaagccat
     4501 gactactgaa ccgatgctta aaaatggtgc tctaatcaat aaacgaatga ccattgctaa
     4561 agctagttga agctgattga tgtcgtttgt cattcgagtg atcaaggtat ctgttccaaa
     4621 atgattcaac tcgttgtgag agaaagtatt gatttttttg atcagctgat ttcgcaactc
     4681 tgtaccaaat ccttgtgaag ctactgaagc gtaatattgg caaatgatag cacaaatcag
     4741 accaatcaca gacatagcga ccatccataa gcccatttca atgatttttc ctcggtcatt
     4801 catttttagt ccattatcga tcaaggaagc catcattaaa ggtaggacaa gttcgaagac
     4861 agcctcaaga aacttgaaga acggacccaa aatgatttgc ttacgataat cttttgcata
     4921 acgtaataac tgaatcatat gattcctcct attatttatt tgtctacagt tgaaaaaaca
     4981 agtactacca cattgtagct taggaaattt agaatgaaaa gagaacaagc cttttcataa
     5041 atgaaagttt tattatgaag agattttaga gattgaatag agtatttttt agaaaaaaag
     5101 aaagagaacg ggaaattcat cccggtctct tcattcctcc aaacgtctat tttgtccgat
     5161 ttttttataa atgaatcgtg tgcaaaaggt ggatcaggta cagaatcatc aatggagcgc
     5221 tatttatttt ttgaaaataa aaaagtagtg attccatgat ttaagtaaaa aacagcgagt
     5281 ataaagcaaa tgatcttagt aatgaataaa atctcataaa atggggaggg cgaagcttca
     5341 ttttccataa aaaccatcgt tatttttggc gaaaaaatta aaagtaacga tgttattaaa
     5401 tctaataaaa gctttgtttt catagtttat tctccttttt taaaagttgt tgaaatcgtt
     5461 ttctttttgt gctataaagt tattgtaaca aattgtttgt gggaaagcta attaatgttg
     5521 aaagaaggga tatgagaatg aaaaaatcgt tgattggttt attatgtgtg gcaactttag
     5581 gggggggtaa gtaatatcgg acagcctgtg tttgctgtag aagtggatgc atcagtgatc
     5641 gacatatcaa atgaaaagaa agagataaac ataagtcccg tactcacttt tgatgaaatg
     5701 gtgcgagaag tagcaaaaga aaacggcatc cctattatcc aagcacaaca agagcttgga
     5761 ttcacagatg aaagtgcaag acaagcacgt acagcaagag caacatacag aacattatcg
     5821 caatctttca cagtcaatgc tagttataga cccacgatgc gcttttattg cgagaccagt
     5881 gaaagtggaa attttagggc tatcaaaaga atcctacgag ttgaaatgat ccgcggatat
     5941 aatggtttgt cgaaacaatt tggtggaact gtttatgttc atttagaaga tgctaaccgt
     6001 attttttaca ttgtaaatgg cgacttcttc aataatggtt caacaacttg gaatgcaggg
     6061 gtgaacatcg gagtaggaag aaacgcttct attaagtttg gtgtaacgaa tacaacctct
     6121 cattatcaat atcgctatgt agaaagccgt ttgcgatttt gatatcaaaa cagcacacga
     6181 aaaagaaaac aaggcaggaa cagtgaagtt tctaccttgt tttcttaatc aatgggaaaa
     6241 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
     6301 ctgaaagcat cataactcgt ttttcctcgt tacgccaata aattgttgtt ataccagata
     6361 taaggatttg taataacaaa aagcagacag aaaaattgag agctgtgcct cctattttgt
     6421 gtggaaggat ttatagagcg caaaaatcaa gataaagata aataaaaatc ctgtttcggc
     6481 tatatattgc gtatctacat cgataaaacg ggtttgttct gtcatagcca tataaagcaa
     6541 aggtaaaaac acaaccacag cgagataaga tccgatgatt tcgcgaaaag ttttccaaga
     6601 agtatacttc ctctttttag atttatccga tttcattgaa tagtcatagc ctccttgttc
     6661 tttctttatt gtttggaaat tccagtataa aaaccggaag gaactgttcg tttttagaat
     6721 aaaaattata agggataagt aagctgctgt ctataacaag acatattttt caactcttta
     6781 ttaaagattt tattgacaaa atgaatccgt tttctatgat ggagtagggg tgaataagtt
     6841 gttaaaaaac gaaattttat gctacttaga gaaccagaca gcctttttcg atctcaacca
     6901 tatgaacgag gtctttacag cacaaagatt ggctgaacat tttggggtaa aaagaaacac
     6961 agtcagtcat tatttgaacc agttgaacga ggagaaacta ctagtcaaaa tcaattctcg
     7021 tccagtggtt tatttccaca aagaagcttt tgaaaaacaa ttttttcagt tacgtacgaa
     7081 tttttatttt tctatcaatg atcttaaaga agaacagcct tttttcgcac aaccaaaaga
     7141 tttattctct tttatgatcg gacacgacgc cagtttgaaa gaaagtatcg aacaaatcaa
     7201 aacagctctt tactatccag atggcggact gccacttttg atcacaggcg aaagcggcac
     7261 aggtaaaagt tatttagtcc atttagtcta tcagtattgt ctacttcatg atttactaga
     7321 ggactctgca ccattcatca ctttcaattg tgcacaatat gcagataatc ctgaactgtt
     7381 gactagcaat ttgtttggac atgtcaaagg agcttacact ggagcagaag aaaataaaaa
     7441 aggggctttc gaagcagcag atggtggaat cttgttttta gacgaggtac atcgcttaac
     7501 accagaaggg caggaaaaac tattcaccta tcttgatcaa ggtgtgattt atcgtatggg
     7561 agaacctaat gtgtcacggc ggatcaaaac acgtcttttt tttgccacaa cagaggagac
     7621 cactagtcat ttcttaacca ctttcattcg aagaatacct atccgaatcg aactacctgc
     7681 actcaatcag cgtagtcgaa atgaacggct agaactggtt tactcttttt ttatcgagga
     7741 acaaagaaaa atggggagcc ctttgtcagt cagtggccca gttttgtcac ttctagcagg
     7801 tcaaaactta tctggaaaca taggagagct gaaaaatatt gtcaaagttt cagcggcaaa
     7861 agcgtatgct gaacaacgtg aacagtcgga gatccatgtc accatccatc atttgcaaaa
     7921 agaactactc gctgagccca ttaccaaaaa tacagcacag caaggaatct atatcacgca
     7981 agaccagacc ttagaacaat tgatggaaaa gcaacaacca gaacagcaac gaatcgttaa
     8041 aagttttgag cagatcctgc ttgatttcaa aaaaaatgag tgtcttctat attcttgcga
     8101 aggaaaacta aaacaagaag tgatccaatt atttgatttt ttgctgtttg agaccagtag
     8161 acaagaaaaa cacgagctgt tggtttattg gactcaatct atccgtgaga cctttcgaca
     8221 aatggagtct gcctatcaaa tcaagttcga tggaaacagt gtctatgcct tgagttacta
     8281 tctttatcag cgaagcactg tcaaatggtt accggaagaa catgcgatta tccaactaat
     8341 caaagaatta gaaaaacagg tttctcaatc ttatccatct agctatcatt atgtccagcg
     8401 aatcttggaa ctgagtaaga ttaaattaga tattgaagtc actagtatgg atcggatttt
     8461 actgacgatc tatctaaaaa aagcaaaatg gtcaaaagaa aaacgactgc caaaagcaat
     8521 gattgctgcg cacggttatg caactgctag cagtatggca aatgtggtca atcgtttatt
     8581 acaagaagat ctatttgaat catttgatat gccgctagat gtcactccac agcagattgc
     8641 ttcagaaatt gtggattatt gcgaaaacag cgatgtttca aatggattga tcatattagt
     8701 agatatggga tctctgaaag aaatccatca gttttttaaa aaacaactat ctgtaccact
     8761 tttgatttta aataatgtga cgacaccttt agccatcaca gtaggggaat gcttgcaaaa
     8821 aaatgcagca ttagaagaaa tagcagaaga ggctgttcgt caaaccaaac cagaatggcg
     8881 gctcctctat ccacaagaaa ataaacccaa agcactcatt actacctgct tcactggaat
     8941 cggcacggcg gctcacctaa gtgcactgct agagaaaagc ttgcctacga cttgtcagtt
     9001 gaaaatcatc ccatatgaat atcagcgatt aaaagataag aaaaacaggg acccactgtt
     9061 ttccatatac gaaatcgtag ggatcatagg taccgcagat cctgctatca ccgatatcga
     9121 ctatctttca ttagaagacc tcatctcagg agagaagatg tctgtactgg cagaatggtt
     9181 ggattctgct atgaatgttt cagataagga aacattcaac cagaacatca ttcgcaattt
     9241 ttcattggag aaagtactgg actctgtgac tattctagat acagaaaagg tgatggagga
     9301 aatcgatcat tttatgcgag aattagaggt gcagttgaat cgaagaatca gtaatcccaa
     9361 aaaactagcg ttatatgtcc atgtgagttg tctaatcgaa cgtttgattc gacaaatgcc
     9421 aattgaaacc tatcaaggat tagagaaatg ggaacagtgt caaaaagaag agatatttgc
     9481 cattaaatct gcatttagtg tcattgaaga gcattatagt gtcatgatcc ctcgttctga
     9541 gattgcctat atctgtgata ttttgagtga aagtacagag ttactctcga tagaagaaga
     9601 attttgaaca taagcaagtt gtggccacta actttggcac acaacttgct tttttagtat
     9661 gtgtaagtaa aaagaggaga tgaaaaagtg gacagagaaa ttatcttagc ttcccatggt
     9721 aaatttgctt cagggatact agattcatta gagctaatct atgggaaaaa tcattctatt
     9781 actgcattgg actgctatac tgatgagaat tttgatttaa cggaatcagt aagtcagctg
     9841 tttatgaggt acaaagataa agaaatcatt gttctgacgg atctttttgg cggaagtgtc
     9901 aacaatgaat tcttacaata tatcaatcag gatcatgtct atcttgttgc cggattgaat
     9961 ttgccactct tgattgagct agtatctcgg ttggacgctg aagtacagac aactgttttg
    10021 atccagcagg tgctaactga ttcaaaaaaa atgatccagt tttgtaacga acggatcaat
    10081 caggaaatag aagaagatga attttaggag gaagaagaat gattgtattg acaagagttg
    10141 accatcggtt attacacgga caagtagctt tttcttggac acaaacaata ggtgcagatt
    10201 gtattttgat tgcgaatgat gatgtgccta ctaatgagat acgaaagaca acgatcaaat
    10261 tagccaagcc gcaaggagtc aaactagtaa tcaaatccat tgatgactcg attgccgcat
    10321 tgaaaagcgg ggtgacagat aaatataagc tattcatcgt agtggaatca atcgaggacg
    10381 catataagct ggcagaagcg tatccagaaa tcaaaaccat caacttagga ggaatcaagg
    10441 cgaaacctgg tactcgcagt attggaaaag cggtgaatat tttaccagaa gaggaggaac
    10501 gattgaaaag attgattgac aaacaaatag aagtggagat ccgtcaagta ccggcagata
    10561 aaaaagtgga cgtaagggaa gttttgtaag aaaggaagaa actcaatgat tgtacaagca
    10621 gtcttattag gaatcgttgc atttatcgca caatcagaat atgcattagg gacatcactg
    10681 ctttctcgtc caattgtgac gggactgttt acaggaatcg ttttgggtga tgtaaaaact
    10741 gggatcatca tgggcgccac tttggagtta gcatttatcg gatcattctc agtaggtgct
    10801 tcgatccccc ctgatgtggt gaccggagga attttaggca cggcttttgc tatcacagct
    10861 ggagctggta cggaaacggc acttttacta ggtttaccaa ttgctacgtt gacattgata
    10921 ttgaaaaata tttacttagg tcttttgatt ccaattatga accataaagc ggacagttat
    10981 gcagaagaag gaaactataa aggagtcgag catatgcact tgcttgccgg gttcggtctt
    11041 tccttgatgc ttggtgttgt agtaatggtt tcgtatatgg tgggaagcaa tacgatcggg
    11101 aatctactca ataagattcc tgacttcgtt cagaatggct tgtcagtagc tacagggttg
    11161 attccagctc ttggtttcgc gatgctcgct cgcttgctga ttaacaaaca agttgcacca
    11221 tactttttct taggtttcgc tatagcttct tacttaaaga ttcctgtgac cggagtagct
    11281 atttttggtg caattttagc cgttgtggta gtcaatatca tgaatgtccg tcaactgcca
    11341 cttcaaacca ctgtagggga ggtcgaagaa gatgaagaat tctgagagta aaaatagcaa
    11401 aataacgaaa aaagaactga atcatgtatt ctggcgttct tttcaaatgg agttttcttg
    11461 gaactatgaa cgacagatga atatggctta tgtttacgca atgattccga ttttgaaaaa
    11521 gttatatcag tcgaaagaag aaatggcagc agctttgaaa cggcatctag agtttttcaa
    11581 cacgacccca catatcgtta cattgatttt agggatcacg gctgcaatgg aggaagaaaa
    11641 taaggatgat cctgaatttg atgtgaccgc gatcgacagt atcaaaacat ctttgatggg
    11701 accccttgca gggttagggg attctttctt ttggggaact ttgcgattga ttgccacagg
    11761 tgtgggcacc tctttagcca tgcaaggaaa tattttagga cctatcttgt tcattttgat
    11821 tttcaatatt ccgcacattc tgttccgcta tctagcaaca ggttggggtt atcgcttagg
    11881 aacgggtttt ttgaaaaaga ttcaggcaaa cgggatgatg gaaagtttga cattaggcgc
    11941 gtctatcatt gggttgatgg tcgtcggagc aatgactgcc acgatgatta ccatcaatat
    12001 tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt caaagtattt ttgatgatat
    12061 tgtcccaaat atcttatgtc tgggtgcatt tggtgtagtg ttttatctat tgaaaaaaga
    12121 agtaaaacca ttaacgattt tgatcggact agctttcttt gggatttttg gttcgtggat
    12181 agggattttt tagaaaagga gcagagaaaa tggttacgat gcaagattat atttatgaag
    12241 aaaaggaagt actaagtacg atcttgaaaa agaatgattt ttcgacaaga aaacaaatga
    12301 aaaaaacaac gaatctgctg atgctagcaa cgggttcttc ttacaacgct tgtttagcag
    12361 cgaagccggc attggaatct tatggcgagc taactgtcga tattcaagaa ccttttcatt
    12421 ttgctcatta tggaaaactt tctccatcaa tcgatactgt gattgctgtg tcacaaagtg
    12481 ggaaaagtgc ttctactgtt gaagcagtaa aaatgatcca aaaacaagaa ataccgatta
    12541 ttgctgtcac aaatgacgta caaagtccac ttgctttaga agctacacag attattgatt
    12601 taggtgctgg gatagaaaca gttggcttcg ttacaaaagg ctattctgct actgtcttac
    12661 agttactact cttagggcta ggaattggga taagtaaaaa taatatcagc aaagaaatag
    12721 aacaaaacta cgtgcaacag ttacacaaaa taatcaatca tttaccagtg attattcaaa
    12781 aaacggaaga attttttgat gaacatcaaa gtttgtttcg tttaacacaa cgcttcatca
    12841 ctattggtta tggtccaaac tggggaacag cgaaagaagc agaaacgaaa ttgacggaaa
    12901 caatccgtgt cccgtcacaa ggatttgagt tagaagctta catgcatggc ccatatctag
    12961 aggctgatgc ttcccatttg ttgttcttta ttgaaggtga caatgaaaat aaggaacgtt
    13021 cacaaaagtt gcggagatat atgagtcagt atgttggaga gactctcaca attaccacaa
    13081 aaaaagcaag agacgaaaaa acacttggat tagcaataga atgtaatgaa tatctgtcta
    13141 tattagcact tgtcgttcca tttcaactat tcgcttataa aatcgcagca gctaaaggaa
    13201 tcgacttgaa caaaaaaatc tttgaagact ttgatacagt cttgaaaagt aaattataag
    13261 aggtggaaat catgttgaaa ttcaatgaag aagaacaaat caaagcaata aaaggggcac
    13321 tagctttacg tccgcaagta gaaaaaatca tcgataaact ttatacgaaa aaattcgatg
    13381 ctgtctatta tttaggaatc ggtggaacgt atgcttcttc gatgcaagct gtcacttata
    13441 tgaatgggaa aagcaacttg cctgtctttg tacagcatgc tgcagaatat tacactactg
    13501 ggaacaaaag attaacaaaa gattctatcg ttgtcgtgtc ctctgttact gggacaaccc
    13561 aagaagtagt gaaagcagta gaagaaatca agaaagtagg agcgacactg attggattta
    13621 tcgataaagc ggagagtaag ttgtctcaac tatgcgattt tgtggttact tatccagctc
    13681 ccggaacaga acaaatcaaa ttttttatgg tagcagatcg attgatgtat ttgcatgggg
    13741 aattcgaaga ttactcagag tactatgaac agctcgaaca ttatttgcca acagggttag
    13801 ttgaagcaga gaaaaaagca gatgcatttg gcttatcatt tgcagaaaag catcgacgtg
    13861 atagcatgca ttattttatc ggagcaggga atcaatgggg agctgtttat tcatatgcca
    13921 tgtgttattg ggaagaacaa agctggctcc catcgaaatc tatccatgct gccgagtttc
    13981 ttcacgggac gttagagatc gttgaagaaa cgaccccagt cactttattt ttaggcgaag
    14041 atgaacagag agtgttatct gaacgtgtag ctaaactttt gccaaaaatt tgtagtaatt
    14101 atacattgat agatacaaaa gactatccag tagaaggtat cagcgaaaaa tatcgcggac
    14161 gtgtgttgtc tttcttattg atgcatgcag tgacacagcg tatcgatgca catgtcgaac
    14221 agatgaattg tcatccatta gagatacgcc gctactatcg tcagtttgat tattaggatc
    14281 gattggatga aaagaatata aaaaataaag agagcagcga caactgctcc ctttattttt
    14341 tagttgtttt ccaagtcagt taatgcatta cgattttgaa cattgacatc gatcacatta
    14401 taaaatgcgt tatctgtgtc agctacatcc caaactgcta aaatgacatg ataccccaga
    14461 cgattgctag gaatattgat tgtatgagtc ggattagtat tagacatatt gccacgatta
    14521 tttacttcac caatgaattc caggtcattt ttattcaatg gggcatttgg attccaacca
    14581 tttttagtca tgtaatagtg ccatttcgtc gttgcgtggt cagctgtgaa atgccaagtg
    14641 aatcggtttg ccccagtagt taagttgttt tttgtccata acgtagctgt ttgtttatcc
    14701 aaaacaaagt cacctactgc accattagca gaagcaatcc gaccatttgc aggtccagcc
    14761 gaagggaatc cttttggtgc ttctaaagat tggggttcgt tgatgatacg accatacttt
    14821 tcaaaagcac tttgccaatt attcctacta tctagagcac cttgatagcc acggctgata
    14881 ggttgtgaaa catagccatg agccgagaca tctacagaag acaaagcggc gattcctgtt
    14941 gtagcaatta acattcctaa tcccaataaa gtactttttt tcatttgatg attcctccag
    15001 ttagataaaa tattttaaga aagggtttcc ttttcataag atagtataaa atgtattatc
    15061 tgaatgttat ttttttgtat ggtattgcaa caaaaataaa aaactagaag aataactatt
    15121 gataaatagc cttatcattt ttatgtatag tttttttcaa aagagaatct ctcaattcaa
    15181 tgatctcttc tttcacttcc tctagtccta gccattcaaa gaaatggatt gcttcgtcaa
    15241 tttcttctag tgctcgttca ttgccatctg ctaatagagc taaagcagtg aatgctttgt
    15301 aacgggctaa gtctaacgta tgtttataac ggtcacttac ttttttccat tctcctaaaa
    15361 atagttttct aagaaaaaca gaagaacgat gaaaagatag atgtatcccg ttcaaaagaa
    15421 aatttgataa taattcggaa taatgagcag tatcgacgta tgctttcatt tttgtgacac
    15481 tacgctgaaa agaatgataa atatactcgt catcgaatat aaataaacaa ttggaaaaaa
    15541 tagaaagttc atagtgtccc catgtatcga taccttccaa gtactttttg ataatagaaa
    15601 cttctgtcaa agtttccgaa cgagtgagtt ttcggtttcc cagatattga tcaattagta
    15661 tacgttgcgt atatagcagg cgataataaa tattcccagt ttttttatat tcttttagta
    15721 gttcattctc ttgctctttt gttaaatatt ttgttgtgat caggtaatta gaaagttttt
    15781 gtttacctgt aagagtattg ttgttcagca aaaattgata ttcttcaagt gtaacattca
    15841 tccgttcaag gtaatccact aacaattcaa aactgatttt tgttcctcta gattcaaaag
    15901 cagctaatgt ccctcgctga gaaagcccct ctgttaattg agcttgtgtc agtccacgtt
    15961 cttttcttag ttttcgaatt aaagaagcat gttttatcat gtaagttacc tccatacgtt
    16021 ttttatgaat ataagataac acaaggctgt tatctaagga cgaaaatgtc aattatgcac
    16081 tatttttgat aaaaagtgct cattctttaa aagcaatttt tatttatgtt gtaataacat
    16141 acaaaaaatg aaaaacagtc gttgttaaaa taaactatag ttaccaaata ttaggaggta
    16201 tgatgaatga aaaaaatttt gtggctgtca ttggcagcaa tcggctgttt cgctggaggt
    16261 ggacaaacta tatatgctgc acaagattct gcggcattga tgccggatat ctcagaaaag
    16321 caaattgctg ttggttatta tcataattgg gtaccagagc aaggtgcggg atatagaggt
    16381 gggaaaccag cagagacaga cttaggaaag atcaatccgt tttataatgt catcgcagta
    16441 tcttttatga aaggcgaagg gattcctaca tttgagccat ttaatatgag tgatgaagaa
    16501 ttcagaagca aagtggctgc cctgaatgaa gaaggacgct cagttatcat ttctctaggc
    16561 ggagcggatt cccatatcga attgtatcga gggcaagaac aagcatttgc agatgaagtg
    16621 attcgattgg tagaaaccta tggatttgat gggttagaca ttgatttaga acaaagcgcc
    16681 attacggcag gtgataacca gcaagtgatt ccagaagcgt tgaaaatcgt gcgagatcat
    16741 tataaaaagc aaggaaaaca ctttattatt tctatggcac ctgaattccc ttatttgaga
    16801 acagcgggag gctatgtccc atatatcact gcattagaaa atgagtacga ttttattgca
    16861 ccacaactat ataatcaggc gggcgacgga ttaagcgtgg gcgacgaatg gattgctcag
    16921 aacaatgaca gtcgaaaata tgatttctta tatggtatgt caaaagcctt gcataacgga
    16981 gagagtggat acattcggat accagcagat cgtctggctt tggggattcc agcaaataac
    17041 gatgcggcag ccaacggtta cgtgcaagat ccatccgttg tctatcaagt attcgaacaa
    17101 atggaaaaag aacgtacacc tttaaaaggt ttgatgactt ggagcgtgaa ctgggacgaa
    17161 ggggtaaact cagctggtat ttcttataat gaatcatttg cgaaagctta tcaaaatcta
    17221 tttaaggaac aagaaccaga tacagagaaa ccttctaaac cgaccaatct tcgaggaaca
    17281 gcaacaagta cgacggtcac attgtcttgg actgaatcaa cagataacgt ccgtgtgtca
    17341 cattataata tttatgaaaa caatcgatta gtaggaacaa gcaacactac gaattatata
    17401 catacaggtt tacaaccaga aacgacttat tcttatacga ttgaagctgt cgatacatca
    17461 ggaaatattt ctgcacgttc tgatgatttg aacattcgca ctcaagaagc agcagtttta
    17521 ccagcacctt ctataccaaa cgggttgagc atcaatcatg tgacacaatc aacagttcgt
    17581 ttggtatggc aagaaaacag cttattggaa gaagtacttt attatgaaat atacagagat
    17641 ggcaagaaga tcgctacctc acaaaccgct gtatttgaag atcaaaattt gacagcttct
    17701 actgcgtatc gctatcaagt tcgtgcagtc aatcagacag gcgcttcttt actaagccag
    17761 tctgtcacgg caagaaccct agacgagaac ggccaaggaa attcttggac agtcggtact
    17821 ttttatcaag taggagacat agtcacatat aatggtattc aatatcgttg cgtgcaagcg
    17881 catattgcag ccgcacattg ggcaccagat acggccttct ctttatggca aaaaataagt
    17941 tgatgatgag gtcgtgatag aggctacttc tgtaacaccg tttatatcaa gcggttgtgg
    18001 aatcagtatt ttgacctaaa tattacagaa acaaaacggc aaaatagttt ttcatatttt
    18061 gccgtttttt aatctaatat cgagggtgag ctgattgaac actgtgtatc aagaaacgat
    18121 aagcctgatg tataactgca tgtcacatca tttactcgga ataaggagga tacgactgtt
    18181 atcaaaattt ctaccatgtg ttgacttaaa caaagagcgt gagacaaaag tgttctttac
    18241 ttttgtctca cgctctaaac acgtataacc ggcggaagca gaagcaactc cttcggaaat
    18301 aagctgaaat tcacaaaaat ttgaaaagta attttcgtga attccttctt atttcttaga
    18361 gttaaacgct tctgtcccag cctcttcttg tatgtagctt caattaaggg ttgttaggag
    18421 aaacttccca gcctcccaat ggtaatggta atggaaacat agtcaaacct cctttctctt
    18481 agtgacaaat aaagtataac aagtgtaaaa agaaaaaaat taaaatgtac gctattgcaa
    18541 cattaaatag attattttaa gtgaaa
//
LOCUS       DADJYG010000045        18405 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.045, whole genome shotgun sequence.
ACCESSION   DADJYG010000045 DADJYG010000000
VERSION     DADJYG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.045"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(110..565)
                     /locus_tag="JZ382_001227"
     CDS             complement(110..565)
                     /locus_tag="JZ382_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427424.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="JZ382_001228"
     CDS             complement(719..1927)
                     /locus_tag="JZ382_001228"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAY2427425.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="JZ382_001229"
     CDS             complement(2039..2371)
                     /locus_tag="JZ382_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAY2427426.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="JZ382_001230"
     CDS             2519..3394
                     /locus_tag="JZ382_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2427427.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="JZ382_001231"
     CDS             complement(3476..4057)
                     /locus_tag="JZ382_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAY2427428.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="JZ382_001232"
     CDS             complement(4063..5322)
                     /locus_tag="JZ382_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY2427429.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="JZ382_001233"
     CDS             complement(5324..6370)
                     /locus_tag="JZ382_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427430.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="JZ382_001234"
     CDS             complement(6605..6880)
                     /locus_tag="JZ382_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAY2427431.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /locus_tag="JZ382_001235"
     CDS             complement(7123..8433)
                     /locus_tag="JZ382_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY2427432.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="JZ382_001236"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="JZ382_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY2427433.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="JZ382_001237"
     CDS             complement(9979..10659)
                     /locus_tag="JZ382_001237"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY2427434.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="JZ382_001238"
     CDS             complement(10708..11343)
                     /locus_tag="JZ382_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2427435.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="JZ382_001239"
     CDS             complement(11409..12812)
                     /locus_tag="JZ382_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY2427436.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="JZ382_001240"
     CDS             complement(12799..13830)
                     /locus_tag="JZ382_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427437.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="JZ382_001241"
     CDS             13861..14082
                     /locus_tag="JZ382_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAY2427438.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="JZ382_001242"
     CDS             complement(14247..14984)
                     /locus_tag="JZ382_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAY2427439.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="JZ382_001243"
     CDS             complement(15137..16477)
                     /locus_tag="JZ382_001243"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAY2427440.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="JZ382_001244"
     CDS             complement(16714..18237)
                     /locus_tag="JZ382_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAY2427441.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5187 a         3923 c         3107 g         6188 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttctt
//
LOCUS       DADJYG010000046        18114 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.046, whole genome shotgun sequence.
ACCESSION   DADJYG010000046 DADJYG010000000
VERSION     DADJYG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.046"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..1146
                     /gene="ugpC"
                     /locus_tag="JZ382_001245"
     CDS             37..1146
                     /gene="ugpC"
                     /locus_tag="JZ382_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY2427442.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVELAFNISKGHFFDKETEQVIKLP"
     gene            complement(1265..1894)
                     /locus_tag="JZ382_001246"
     CDS             complement(1265..1894)
                     /locus_tag="JZ382_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427443.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFTNWRFQGFNFVIGVIHSLVEMLVVSIFYFIGNMPDTYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKGNRKESIQGYAKKTIY"
     gene            complement(2207..3091)
                     /gene="rsmA"
                     /locus_tag="JZ382_001247"
     CDS             complement(2207..3091)
                     /gene="rsmA"
                     /locus_tag="JZ382_001247"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY2427444.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDERTNVIEVGPGIGALTEQLAMHAKQVVAFEIDDRLIPVLADTMQPYDNVTIIH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDTPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKTWLSKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENKQ"
     gene            complement(3084..3653)
                     /gene="rnmV"
                     /locus_tag="JZ382_001248"
     CDS             complement(3084..3653)
                     /gene="rnmV"
                     /locus_tag="JZ382_001248"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAY2427445.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEDILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            complement(3646..4422)
                     /locus_tag="JZ382_001249"
     CDS             complement(3646..4422)
                     /locus_tag="JZ382_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAY2427446.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAATNVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVATQTWSNAHRLFGLSEHG"
     gene            complement(4577..5899)
                     /locus_tag="JZ382_001250"
     CDS             complement(4577..5899)
                     /locus_tag="JZ382_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2427447.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVVWGLLTPGQGTVQLFPIEAGKEVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGEI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAI
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            6074..6661
                     /locus_tag="JZ382_001251"
     CDS             6074..6661
                     /locus_tag="JZ382_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427448.1"
                     /translation="MDFKRSYPKLADYLSNRIDFSRKEQQQSRWHWFTLILFGLLTIA
                     TLSQKLILLTVFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALREIMHWNNHWFSAGALLLGLILLHVMLTKLYQRYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            6811..7083
                     /locus_tag="JZ382_001252"
     CDS             6811..7083
                     /locus_tag="JZ382_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427449.1"
                     /translation="MNQLKTARPLIIMLLLSVFTIPISLFLNWQTDERITNILFNYSQ
                     PLFLLFLGSCRFHRWVKLVLLFIGYILYGYMCLYYMIGFHNHHWGN"
     gene            complement(7138..9147)
                     /gene="metG"
                     /locus_tag="JZ382_001253"
     CDS             complement(7138..9147)
                     /gene="metG"
                     /locus_tag="JZ382_001253"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY2427450.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            9416..9694
                     /locus_tag="JZ382_001254"
     CDS             9416..9694
                     /locus_tag="JZ382_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427451.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     VAWLLRFVIDAYIVICGFFAVREERYKVLPFYLLFFLFNLVLPFIFHI"
     gene            9792..10613
                     /locus_tag="JZ382_001255"
     CDS             9792..10613
                     /locus_tag="JZ382_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAY2427452.1"
                     /translation="MNRFASKEEEKLYYEHEASEEDFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHGNKINL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(10896..11906)
                     /locus_tag="JZ382_001256"
     CDS             complement(10896..11906)
                     /locus_tag="JZ382_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2427453.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCVRFTYNHLLKERQKSWQQT
                     GVVDFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTHTVYLKNGHLKLPKQKELIKINQHRPVEGTIRSATISARYNEEFYVALL
                     CDVSSIKKESSAKWIGIAYHPKTLIETSHPIEVTLPKFDQTEEKLQHAQRKLSVKVRS
                     AHHRKTRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     DAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNFVRKSQVLEQLGRHSVI
                     KE"
     gene            complement(11918..12871)
                     /locus_tag="JZ382_001257"
     CDS             complement(11918..12871)
                     /locus_tag="JZ382_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427454.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDVTKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSPEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQREHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGKSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(12861..13931)
                     /locus_tag="JZ382_001258"
     CDS             complement(12861..13931)
                     /locus_tag="JZ382_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427455.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGKDIVEKTEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPGEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(13937..14980)
                     /locus_tag="JZ382_001259"
     CDS             complement(13937..14980)
                     /locus_tag="JZ382_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427456.1"
                     /translation="MEMTPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMFLLLVIILISVVTIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVSFFLGTDGLGRDVLSRLFMGTRISLLIAFIAALLDV
                     TIGVAYGLISGLLGGRVDNAMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(14981..15922)
                     /locus_tag="JZ382_001260"
     CDS             complement(14981..15922)
                     /locus_tag="JZ382_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427457.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            16006..16353
                     /locus_tag="JZ382_001261"
     CDS             16006..16353
                     /locus_tag="JZ382_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAY2427458.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNVINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSTASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(16423..18084)
                     /locus_tag="JZ382_001262"
     CDS             complement(16423..18084)
                     /locus_tag="JZ382_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427459.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         5141 a         3728 c         3347 g         5898 t
ORIGIN      
        1 gttaataaaa ctaaaaaaga aagtgtggaa cgtattatgg tagaaatggc tctgaaaaac
       61 gtatataaaa aatatgataa tgcagaaaat tattctgtga ctgattttaa tctatcaatc
      121 aaagaccgtg aatttatcgt ttttgttggt ccttccggtt gtggaaagtc aactactttg
      181 cgaatgattg cgggactgga agatatctct gaaggagaac taaatattgg cggaaaagtc
      241 atgaatgacg tggcacctaa agaccgtgat attgccatgg ttttccaaaa ctatgcattg
      301 tatcctcaca tgactgttta tgataatatg gcttttggtt tgaaattacg taaatatgat
      361 aaagcagaca ttaaaaagcg tgtggacaat gcagcagaga tcctcggact gacagaatac
      421 ttgaaacgaa aaccagctgc tttatctggt ggacaacgac aacgtgtagc attaggacgt
      481 gcaatcgttc gggatgccaa agtcttcttg atggatgaac cattgtcaaa cttagatgct
      541 aaattacgtg tagccatgcg tgctgagatt gccaaacttc accaacgtct tgaaacaacg
      601 acaatctatg ttactcatga tcagacagaa gcgatgacta tggctgaccg aatcgttatc
      661 atgaaagacg gatttatcca gcaaatcggt acaccgaaag aagtttatga tacaccagtc
      721 aatatgttcg ttgccggctt tattggatct cctgccatga acttctttaa tgtgacattg
      781 aaaaacggtg tcatcacaga tggacatggg cttaagcttc gtattcctga agggaaaaac
      841 aaagtattag ttgaaaaagg ttatgaagga aaagaagtta tcttcggtat tcgtccagaa
      901 gacatccaca gtgaacaagt agcccttgaa gcttcacctg aagccgtagt caaatcagaa
      961 gttgtcgttt ccgaattact tggtgctgaa acgatgcttt acacaaaaac tggttctact
     1021 gagttcatct caaaagtaga tgcaagagat ttccataaac caggcgaaat agtcgagctt
     1081 gccttcaaca tcagcaaagg ccacttcttt gataaagaaa ccgaacaagt catcaaactt
     1141 ccctaatctg ccccctttag agctacgcca gaaaatagtg gtgtggctct ttttaggaca
     1201 aaaaactctc ctcttagcgg atttattatc cgttagcaga tgagagtttt tctttgatag
     1261 ttcattagta tatcgttttc ttcgcgtacc cttgaatcga ttctttccga tttccttttt
     1321 ttgctgccgt aaataccggg atatcaaaat atttgctgag tgttcctgct ataaaataag
     1381 caatattata atcaaccaaa ctgtggatga gtccaccaat tcccatcaag atgaacactg
     1441 tataaaaata cccttgagag taatacgtgt caggcatatt accgatgaag taaaagatgc
     1501 tgactactag catttctact aaagaatgaa tcacacctat cacaaaattg aacccttgaa
     1561 aacgccaatt agtaaaagta aactgtccat tctttaaaac gatactcgga tgcttctgca
     1621 agtaccatgc cccaatcaat gcaaatacaa gatgtgacaa tgcgcgcaat gcaatgataa
     1681 ctggcgttgt caaaaagaaa ccgaatgtcg tacctagcgc aacagccact gcaactcctg
     1741 gtgtaaaaaa catcgcaata aataatggca catgagacgc taatgtaaaa gaagcaggtg
     1801 gaatcatgat tttaggcata accattggga taatgacccc catcccaatc aataaagcag
     1861 aaatcgttag ttttcgaact gaattttttc tcattttttc tctctcctaa tatgtcttga
     1921 cacttaaatg tcgacttagt ataacttact tactaatgac tgtcaagaca catattcttt
     1981 tctaaatcca tacaaaatga gtgtgagtat caagtataag aatttttgat caagagacaa
     2041 aaataggaga tgtgacaaaa tttgtgtcac atctccttgt tcataatggg aatcaaaagc
     2101 ttgcctcagc ggcgataaga ccaaccaaat caaaaatagg agaagctgtt ttttgaaatt
     2161 gctccttgta cctcagagtt aaatggcagg ctcacaacct ctttttttac tgcttatttt
     2221 ctgacatcgc attgcttaat gcggcaaatt cttgtaatga caacgtctct ccacgacgtt
     2281 ttggatcgat tccagccgtt tccaagcttt ttgataacca tgtttttgtc tgatcatctt
     2341 tgccatacga atgctgcaaa ttattccaaa gcgtttttct tctttgctga aatgcagctt
     2401 ttgtcaatcg gaaaaatgct ttttcgtccg tcacttctac tgccggtgta tctcttcttg
     2461 tcagtttcaa aatcgctgaa tctacatttg gctgagggac aaatactgtt tttggtacga
     2521 tgaaggctaa actggcttcc atataatatt gaacagcaat cgataatgaa ccgtatgctt
     2581 tcgttcctgg ttcagcagag attcgatcag ctacttcttt ttgcatcatg acgactaatt
     2641 catctacgac caaatccgat tcaagaaaat gcatcatgat gggcgtcgta atataataag
     2701 gtaaatttgc tactactttt aacggaagct cttcatgaaa tgtttctctt acggctgttg
     2761 ataaatccgt cttcagaata tcttgatgta tgatggtcac attgtcatag ggttgcatcg
     2821 tatcagccaa tacagggatc aaacgatcat caatctcaaa tgctacgact tgctttgcat
     2881 gcatcgctaa ttgttctgtc aatgcgccga ttcctggccc aacttcgatc acgttcgttc
     2941 gttcatctat ttgcgcagtt tctacgattt ttcttaaaat atttggttca gtgagaaaat
     3001 tttgtcctaa acttttttta aatgaaaaac catgtttcaa taaaatctct tttgttctag
     3061 agggtgtcgc gatttcacga tattcactca tgttgttcct ccactatttt cattgcttgt
     3121 gcgagctctt tcggcgtgat acgaaacatc ttcaaacgtt tttccaattg tttgccattc
     3181 gtataaccga tacgcaactc atcacctagt ttttctcgtt tttcttttgc ttgtgctcca
     3241 gctattaaac catagtctaa aaggtcttgt ctagtgattt cagaaacagc cgtttgggaa
     3301 atatctggcg tcacaacctt tttcaatgct tctaaaatag cagcatcact tgcatgctca
     3361 acaccaagac ttcccttttt ctttggttta gcttgtgtac gcggtaaaaa agcatgtttt
     3421 gcttggggaa cagcttccat aatgattttt cttatttttt ctcctgaaaa atcagggtcg
     3481 gtaaaaacga tcacccctcg agtctcttgg gcatgttcaa tttgcatcag gatatcttca
     3541 ttgatagcag aaccaatcgt ttctatcgta tctgcatcaa ctacttcccg aagccttctt
     3601 gtatcgtctt ttccttcaac gactataatc tcttgaatct tcatcttatc catgttcgct
     3661 tagaccaaat aatcggtgcg catttgacca tgtttgggta gccacttctt ctgttggcag
     3721 attccgaagt tcagcaatct tttctactgt ataacgagta tagcctggtt catttcgctt
     3781 tcctctaaaa ggaactggtg ccagataagg ggcatctgtt tccacaagta atcgttcgat
     3841 tggtacattt gttgcagcct cttggacgtc taatgctttt ttgaaagtga cgacaccgct
     3901 aaatgatata tgcatcccta agtcaagaaa tcgttttgcc cattcataat caccgctgaa
     3961 actatgcatg attccaccaa tatctcgaac atcttcttct tttaggattt gatacgtgtc
     4021 ttcaagtgct tctctagtat ggatactgat tggaaggttc atttcttttg cgatagcaat
     4081 ctgtctgcga aatactttat cttggacttc tttcgggtct tccatccaat gataatccaa
     4141 gccaatttcc cctaatgcta caacctttgg caaagtcaat tgttcttgta atttattttc
     4201 gatttctttc gtataactgc cagcctctgt cggatgccag ccgataatac tgtaaatatt
     4261 tggatagtca tggcttaatt gcaaagactt ttcaatcgtt ggcgtatcga agcccacgac
     4321 cgccattttc gttaccccca tttcttgtgc tcgctgaatc gtttctggaa tatcttcatt
     4381 gaactgctca gcatttaaat gggtatgaga atcaaatatc atgtttatct ctccttttca
     4441 ttcttacttt agcacataag cactgcttgc agaagtagca aaaatcaaaa tagaagaact
     4501 tgttttgttg aaatagaaaa agcacgaaga tccagaggac ttagttcgta ctttttctgt
     4561 ctgttgttga aatattttac tcttctgagc gttttttctt tttttgataa tagactggaa
     4621 taccaatcaa ggtaacacct ataccgatca atgctaattt cgtttgcgtc atcatcgtca
     4681 tacctaaaat gaaaatagca ccgagaatcg caataatcgg aacgattgga taaagtggta
     4741 ctttatatgg gcgtggcaat tccggcgctt tttttcttaa aataaagaca ccgataaaaa
     4801 tcagtaaact gaatagccac atcacaaaaa tcaacatgtc ggttaaaaag tcgaaagaac
     4861 caaggctcat cataatacaa gctactgcta actggaaaat cgcaggaaca taaggtaccg
     4921 taaatttctt tgaaagcttt gtcagctgtt tgctgaaagg tagctcgtct tccaaagcca
     4981 tcgcataagg cacacgaatc ccagttaagg tatacccatt caatgccccg tataccgaaa
     5041 tcaaaatccc aatagtgacc agttttccgc cgatttctcc aaaaatgacg tctgatgctt
     5101 cagaagctgc gttcaaatta ccggcaagat gatcaatcgg cagagttttc aagaaaacaa
     5161 aattgatcaa taaataaacg acagtgacaa aactcagccc tagtataatc gctttaggca
     5221 aatctttttc cggccgtttc atttcaccag ccattgcgcc gacgcctaac cagccgtcat
     5281 aagcaaacaa tgttgctagc aacgcactgc tcaagccttc gacaaaagta acctcttttc
     5341 cagcttcgat tggaaatagc tgaacagttc cttgtcctgg tgtcaataat ccccatacga
     5401 caatcacggc aattggtatc aatttaacaa tcagcgtcac tgattgtaca gacgcaccga
     5461 cttttgttcc taataaattc aatcctgtca cagaaacagc ggtaatcact gcaatcaaca
     5521 aaagatagtc ggtagataat tgtaataagt tcgtcaattg tgtcgcaaag ataatcgata
     5581 atgcagcaat attagcgggg aaataaatca agctttgcgc ccaccctaat aaaaagctgg
     5641 gcagctttcc ataagctgct tcaatatatt tgacagctcc accagtttct gggatcgctg
     5701 tagctaattc agccactgtc aatccagcac agatcgttaa gaaaccaccc aaaagccaag
     5761 ctagtaatgt caaacttgta gactgcgtgc ttgcagtaac agcagcggtt ttaaagaaga
     5821 cccctgcgcc gatcacggtt cccataacgg tagctaatgc gccaaacaga ttgatttctc
     5881 gttttagttt gcctttcatt cccatcctct ttcttctttg aaatttggta taattttcag
     5941 tataactgag aatattttaa tttaccattc ttttttctcc actttcaatg aaaaactccc
     6001 tcttaagacg gatgtatttc ctctttattt tgcgtatact aaacctacct tttccacgaa
     6061 ggaggaacag tttatggatt ttaagcgttc ttatccgaaa ttagcagatt atttatccaa
     6121 ccgaatcgat ttttctcgta aagaacagca gcagtcccgt tggcattggt tcacgttgat
     6181 tttatttgga ttattgacca ttgcgacatt atcacaaaaa ttgatattgc tgacggtgtt
     6241 catcgcaatt gttgcattga tcaaaggtcc cgtgatgatt ttgtggggga tgttgtattc
     6301 ctttctagtg gggctattcc cacctatcgg tattttgctt tcagcgattt tctttttgtt
     6361 gaacattgga acatttacaa agaattggcg gatgacctta gtaggaatct atttttattt
     6421 ttatcccttc ggagtgatgg cacttcgtga aatcatgcat tggaataatc attggttttc
     6481 agctggtgcc ttgcttttag gattgatctt gcttcatgtg atgttgacga aactgtatca
     6541 gcgttatggt attggaagaa cgattttctg gtacgtcttt tcgattccat ttgcactgct
     6601 tactgctctc ttgccaagcc gtttgaagac aaaaataaaa ggttatcata aaatcaaata
     6661 ataggatttt tacaaaataa agaaaacaat catcgatttt gacctcgatg attgttttct
     6721 ttattttgaa tactattagt agatgtaaac aaattattgc tttcaaaatc cttggatgta
     6781 agagacaata aaataaaaag gagtacccag atgaatcaat taaaaaccgc ccgtccactt
     6841 atcattatgt tgcttctttc tgtctttacg attcctatta gcctttttct taattggcag
     6901 acagatgaaa gaatcacaaa tattttattc aactattcgc agccactttt tcttttgttt
     6961 ttaggaagct gcagattcca tcggtgggta aagctcgtcc ttctttttat aggatacatt
     7021 ttatatggtt atatgtgtct ttattatatg attggcttcc ataatcacca ttggggcaat
     7081 tgacaggaat aaaaaaaact cgcaagcagc attgtcttgc gagtttttct tatgatttta
     7141 tgcgatctct gagccattcg gcatggattt tggtgcttca atcacttgta aagagccatc
     7201 tggtgcttct gcggaaagga tcattccttg actgatctgt ccacgcatct ttcgtggttt
     7261 cagatttgcg acgatcacta cttttttccc aatcagttcg cttggatcag gatagaattc
     7321 agcgattcct gacaaaattt gacgatcttg gctgtctcca gcatctagtc ggaattgcaa
     7381 caacttatct gccccttcta ctttttggca gttgatgact tctgctactt ttagttctac
     7441 tttttcaaat acatcaaatt tgatttgttt ttctttcgtt gaaacaagtt ctgtttcttc
     7501 tggatcccat ttgactgtat cttcgtttgt ttgagtacct tcagacattt tttcttggat
     7561 aaaagcaact tcttcttcca tatccaaacg tgggaagatt ggtgtacctt ttgcaactac
     7621 ttttgttcct gaagggaatt cgccaaaatg caatccttca agattcatcg tttcagagtc
     7681 caatcctagt tgattgaaga tcttctctgg cgtttctgtc atgattggtt gaagtaaaat
     7741 cgcggcgata cgcaaggatt cagctaaatg gatcatgaca ctattcaatt cagctttctt
     7801 ttcttcgtct tttgctaata cccatggttc tgtttcgtca atgtatttgt ttgcacgaga
     7861 aatcagcacc cagatttcag ctaacgctgt attgaattcc attttttcca ttgcatcatg
     7921 gtaacgtccg acaacgttag cagccgtcgt agacagttca ctgtcaaatg gagtgacaag
     7981 tgaggcataa tcaggaacga gaccatcaca atatttgttg atcatagcaa tcgtacgatt
     8041 caataagttc cctagatcat ttgccagatc ataattgaca cgagaaacaa aatcttctgg
     8101 tgtaaagacg ccatctgacc caaatggcac agcacgaagc aaataataac ggagtgcatc
     8161 taaaccataa cgttccacaa gcatttcagg ataaacgaca tttccttttg atttagacat
     8221 ttttccatct ttcatcaata accagccatg accaaatact tttttaggta gaggcaaatc
     8281 aagagccatc agcatgattg gccaataaat cgtatggaag cgaacgattt ctttaccgac
     8341 catctgaaca tctgcaggcc agtatttttc gaatagactt tcatcctcgg acccgtagcc
     8401 tagtgcagtg atgtaattgg acaatgcatc gatccatacg tacaccacat gtttcggatc
     8461 actcttgact ggtacccccc aagtaaaggt tgttcgtgaa actgcaagat cttctaatcc
     8521 tggtttgata aagttgttga tcatttcatt tttacgagat tctggttgga tgaattcggg
     8581 atgttcttca tagtattcca acagacgatc tgcgtatttg ctcatgcgga agaagtaaga
     8641 ttcttctttt accaattcta cttcgtgtcc gcttggcgct ttaccgccga tcacattacc
     8701 ttcatcatct ttgtacactt ctgcaagttg tgtttctgta aagaattctt cgtcagaaac
     8761 agagtaccag ccttcatatt cacctaagta aatatctccc tgttctaata aacgatcaaa
     8821 tattttttga acagctgctt tatggtaatc atctgtcgta cgaatgaatt tgtcataact
     8881 gatatcaagc gttttccata attttttgac atcagccgcc attttatcta cgtattcttg
     8941 cggttttaca cctaattctt ctgcttttgt ttcgattttt tgaccatgtt catccactcc
     9001 agtgagataa aacacatcaa agcccatcaa ccgtttataa cgagcaattg cgtcacatgc
     9061 aatcgtagta tatgagttgc cgatatgaag tttgccgctt ggataataga ttggcgtagt
     9121 gatataaaaa gtttcctttt ctgccacggg gctcttcctc ctttatcgca tgatgcgaat
     9181 tcgtttcctt gctagtatac cataaaaaaa gcatgctgtt agcttttttt caaaatgaaa
     9241 aagtctggat aaatgacttg ttctctacga tgactccctc cttcaaaagg aaggacagag
     9301 ggaataaatg ctatttttca tcttgtttca tgtttagtcg gaaacggctt tttatttctt
     9361 tcctgactcg tggtataata aagactatct attttagtaa gggactggaa cgatcatgca
     9421 gaagaaacat ctttttttta cacttagtat tgcttttctt tcattagcac atttgatatt
     9481 cagctatttt tatattcgta tgtatggtta ttttaacctt catgggcatt tgaacagttt
     9541 catgacagtc gcatggcttt tacgttttgt cattgacgca tatatcgtta tttgcggctt
     9601 ctttgccgtt cgtgaagagc ggtataaagt attgccgttt tatctgcttt tctttttatt
     9661 caatctagta cttccattta tttttcatat ttaaggaaga accaaggatt tttcaagtaa
     9721 atccttggtt ttttttcaga gtctgctaca ataaggtaaa aagtactttt aatgtcaacg
     9781 gaggtggtct gatgaaccga tttgcttcta aagaagaaga aaaattatat tatgaacatg
     9841 aagcaagtga agaagatttt cttaactggt atcaaaaaca ggaacgtcca gaatatgaca
     9901 agccctcttt gactgtcgat atcgtactga tgtgctataa caaagaagaa gatcagctga
     9961 aagtattgct gatccaaaga aaagggcatc cttatcgaaa ttcatgggca ttacctggtg
    10021 gttttgttca aaaggatgag tcaactggag aaagtgtctt aagagaaaca aaggaagaaa
    10081 ctggtgtagt tatctctaaa gaaaacattg aacaactgca tacattcagc acgcctaatc
    10141 gagatccgcg cggttgggtc gtcacagtca gctacttagc tttcattgga gaagaaccgc
    10201 tgatcgccgg agatgatgct aaggaagtac gctggttcac attggaacgt cacggcaaca
    10261 agatcaattt atcgagcgga gaagtggcta tctcacttga cttagtcact ggagagtctt
    10321 ctggcaaaga tactttagct tttgaccata gtcaaatcat tttgaaagca ttcaaccgtg
    10381 tagtcaataa aatggaacac gaaccgcaag ttttgcaagt tttaggtaaa gatttcacga
    10441 ttacagaagc tcgaaaagta tttgcaaagt tcttaggaat cgattataaa acgatcgatc
    10501 attccaattt taagaaagct atgctccaat atttcgacga gattggcgaa cgtcctgtag
    10561 gaatcggtcg cccttcaaaa atctatcagc tcaagccggg acatcatgag taaggattgt
    10621 gaagaaaggg ctccgttctg aacactcaag ataaatggaa agccgctctt tgtgttttgc
    10681 gtttttcgct ccatactttg gcatatcagt aaaattcttc gaataaaaaa ataacaatag
    10741 cagaaaaaac tcgtacgaat tttttctgct attgttttat tggtttgtca taattagtga
    10801 gcttttcttc aaaagaaaat tcctagaaac aaaagtcaat cagacttttt attaacaagg
    10861 cgaaccctaa cgctcctgtc agtttcattc attttttatt ccttaatgac cgaatgcctg
    10921 cctagttgtt ctaacacttg gctctttctt acaaaattgc tttcttctgc tccgtcggta
    10981 ttgatcaaaa gaaatttttt attataccat tgggacttat aacgcaattt tgttattaat
    11041 ttatgccagt catgaataga aaaatcagca tgtgcatctt cttttggaaa agattctaca
    11101 aataagtagt catattgttt gaccaatttc ccagataatt gttcaagata atcttctcgc
    11161 tggttctttt gtttcagata aagatccatt acttttctct tttgtttctg ataattgctt
    11221 gcctgatcta acctggtctt tctatggtga gcggagcgga cttttacgct taatttccgc
    11281 tgtgcatgct gaagtttttc ttctgtctgg tcaaattttg gtaaagttac ttctatggga
    11341 tgcgatgttt cgatcaatgt ttttggatga taggcaatcc caatccattt agctgacgat
    11401 tcttttttta ttgatgaaac atcacaaagt agtgcgacat agaactcttc attatatcga
    11461 gcagaaatcg tagcggaacg aatcgtgcct tcgactggac ggtgctggtt tatcttgatt
    11521 agttcttttt gttttggtaa ttttaagtgg ccgtttttca gatagaccgt atgagtttga
    11581 ttattcgtcg tataagattg ccacatactt tttttcgttt taagctttgg gaagctggca
    11641 cgtcccttga aataattgcg aaaagctcgg tccaaattaa gctgtgcatt tgctaaagca
    11701 agactgtcta cctcttttaa gaatgggtat tcttttttta acgtagcagg cgtgagcgaa
    11761 aaatcgacca ctcctgtttg ttgccaactt ttctgccgtt ctttcagcaa atggttatag
    11821 gtaaatcgaa cacagccaaa agtacggatc aattgctgtt tttgttcttc tgtcggatac
    11881 atacggaatt taaaagcttt tagcatctat gtccctccta gttagcaacc agaagagatt
    11941 ccttttgtcg ttttaatttt tttgcgtaat atgggacttc ctgctcagtt gcatagacaa
    12001 aatgttctgg tgcgatttca cgaagctgac gtacttgtcc gtcatctggt tcgggttgat
    12061 atttgattct tctgcgcttt ctctcatgat ctggatctgg cagaggaata gcagataaca
    12121 aactttctgt ataaggatgg acaccgtgat tatagatttc atcacttttt cccatttcta
    12181 ataacaaacc gttatgcatt acaccgattc ggtcgctgat atgtttaacc attgataaat
    12241 catgcgcaat aaatagatag gtcaattgat gttctcgttg gagatcttgc aataaattga
    12301 caacttgggc ttggatggaa acatctaatg cagaaatcgg ttcatcgcaa atgataaatc
    12361 gcggttttac tgctaatgct cgtgcaatac caatacgctg gcgctgtccg cctgaaaatt
    12421 catggggata acgagtgccg tggctcggat tcaatccaac ggttcttaat agatcatcga
    12481 cttttttggc acgttcctct ggtgatttag ctaacccatt gacatcaatt ccttccgcaa
    12541 tgatatctct gactttcatc cgaggattca acgaagcgta tggatcttga aagatcatct
    12601 gaacatcatg tcggaatttc gtcaatcctt ttttgtcttt gatcttcgtt acatcttgac
    12661 cgtcgaataa tatctcaccg tcagtaggtt cattcaaacg aatgatcgtt cggccagtag
    12721 tggattttcc gcttccagat tcgcctacta aaccaaatgt ttctccttca tagatgtgaa
    12781 agctgatgtc atttacagca tgtacttcat tcttttttcc tacattgaaa tactgtttca
    12841 gtcctttaac ttcaagtagt ttatttggca tcagcaattc ctccttgacc ataagtatgt
    12901 ttttgtttct ctttaaaaat agcccagcga cgttggattt ctcctggcgg cgtcacttta
    12961 ggcgcttgtg gagcaagtag ccaagttgct gccttgtgtg tttttgatag ttcaaaaaat
    13021 ggcggttctt gttctgcatc gattttcatc gcgaattcat ttcttgggta gaatgcgtct
    13081 ccttttggtg gattcagtaa atccggtgga gaaccaggaa tggcatacaa tcggtcgttc
    13141 gcactgtcta atgtcggcat tgacccaagc agtccccaag tatagggatg ttgcgggttg
    13201 tagaaaattt cttcggatgt tccaacttca acgattcttc ccccatacat aacagctaca
    13261 cggtcagcaa cgttcgctac tacaccaagg tcatgagtga taaagatgat tgatgtatcg
    13321 atttttgtct gaatttcttt cagtagttcc aatatttgtg cttggatagt cacatcaagt
    13381 gctgttgtag gttcatcagc aatcagaatt tccggataac ataccagtgc gatcgcaatc
    13441 acgatccgtt ggcgttgacc tcctgaaaac tgatggggat aactattgat acgtttctca
    13501 gcttcaggta taccaactaa tttaagcagt tctaacgcag ctttttgtcc ctcttttttc
    13561 gaaactttat tatgctttct aagtgactca gcaacttgtt tcccaatagg catcgttgga
    13621 tctaaagagg tcattggatc ttggaaaatc attgctattt cttttccgcg gattttttgc
    13681 atctctcttt ctgtcttttc aacgatgtct ttacctttaa aaaggatttg cccagcatct
    13741 atattggcat tgctcgacag caagcgcatg atgcttctgg ttgtcactga ttttccgctt
    13801 ccagattccc caacgatcgc caatgtttct cctttattta aatgaaaatt gacaccgcga
    13861 atcgctcgta cctttccagc atacgtatca aaagagattt ctaagtcttt cacttctaaa
    13921 attttcgtca tcgtcctcac tctttcattt taggatcaaa ggcatcacgc aatccatcag
    13981 caagtaagtt aaagccaatc atgataaccg ataaagttac tgccggaatc cataatagat
    14041 aaggcagata caggaatgtc ttgtaccctt cgctcaacat cgttcctaat gaagctgacg
    14101 gaggcgtaag acctaaaccg ataaaactta aaaaggcttc gaagaaaata gcagatggga
    14161 tgctaaacat catttgaata ataatgacac tggagatatt cggcaaaata tgtttgaatg
    14221 caattttcat tcttgactca cctaatgtca atccagctag aacatattct tgatctttta
    14281 atttcattgt ttgtgcacga acgatacgtg ccattggtat ccagtttgtg atggccatag
    14341 cagccacgat ggaaaaaatt cctggttcga agacgaccaa catcaaaatc atgacaacga
    14401 ggtttggaat tcctgacaag atttccaaaa aacgttgcat cgcattatcg acacgtccgc
    14461 ctaacaaacc agatattaac ccgtaagcta ccccaatcgt tacatctagt aatgcagcaa
    14521 taaatgcgat cagcaacgag attctcgttc ccataaataa acggcttaat acatcacggc
    14581 ccaaaccgtc tgttcctaaa aagaaactta cgtttgaagg aacgtttgct tgagcatatt
    14641 tatcaaccag ttctccgccg accattgctt ttccattcaa tccatcgata ttgattcccg
    14701 gaatacgagg aggcaaattg atataagcca cgttttgggc agttggatca tgtggcgaaa
    14761 caaagatcgt gaccacagag atcaaaatga tgaccaacag taaaaacatt gaaataacgg
    14821 cagctttgtt tttctttaaa cggcgccatg aatcttggat aaaattcaaa gaaggtgcag
    14881 taatctgctc acgttcttct tcggtatttt tttgtaatgg aagaaactca tccgcaggaa
    14941 tatctgcgac tgtttcataa cgcttgggtg tcatttccat ttatcctcgg ctcccttctg
    15001 aaacacgaat tcttggatca actagaccgt acagcagatc aacgatcaaa ataacaaaaa
    15061 tcaacataaa tgagtaaaga atcgttacag ccatgatcgt aggataatca tttgttgtga
    15121 tcgacttaac gaattgttca ccgataccag gaatagcaaa gatattttct accaccagtg
    15181 atccggtcat caatgcgaca gccaagggtc ctaataaagt catcaatggg atcaaactat
    15241 ttcgaagacc atgtttaaag gcgatttccc aacggctcaa tccttttgct tttgctaatt
    15301 ccacgtaatc actatgcagt acttccacca tttccgtccg gataaatcga gcagaatcag
    15361 caagcggcga catggccaat gcaattgtcg gcaagatcgt gtacacaaat ccatcccatt
    15421 ttgcgattgg taaaacttgt aatttgattg caaagatata ttgtagtaaa acagcaaata
    15481 caaagtttgg aatggaacga cctaatattg ctacaagtgt agaagatgta tctgcccatg
    15541 tattttgctt catggcagaa atcgttccta aaatcgttcc taggactgtt ccaaagataa
    15601 ttgcctgtaa ccctaattgc aaagacggtc cgattcttcc agctaacaaa tgtgcgactg
    15661 gctggttttt aaattggaag gaaattccaa aatctccttg aagcaaatta gaaagataaa
    15721 tcccatattg tacgataact ggtttatcca aaccgacttg tttatttagc atctcgattg
    15781 tttcaggact caaacgttcc tgattcgtat atggcgtgcc cggcagcaat tgcatcaaga
    15841 aaaaggtgat tgttgcaatt aaccaaagcg tgatgatcat gaaaaagaca cgttttaata
    15901 catatttgat ataactattc actaatgttt ccccctagct tttgacttct gacagatgtt
    15961 tcgctacatt attagataat gaagcaaaaa taaaggagca atcaaatgaa aaaaaaatct
    16021 atcccctacg cagtagcttt tctactgatt ctggttattt taataaaaaa cgtcatcaac
    16081 cattccttta cactcattca attatctaat gatctattct tatggtcatt gccgtttctg
    16141 attatcggcg gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg
    16201 gtccatcttg cacgaacaag gaaccgaaag aaaaaaccag aattttcctc gctctcgact
    16261 gccagctacg gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct
    16321 gctattttca tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat
    16381 ttgccgcagc ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt
    16441 ccatttgtaa tcccatgaag cgcctgcagc atggtgaacg atacctttga ttttttcatt
    16501 cactaggtga ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat
    16561 cttgattgct ttttggaaat catcccaacg ggcttgttca tcgccagcat ctgttgatga
    16621 agctgcttta actgcttcgt catattctgc attgctccaa cgaccacggt tgtatgaatt
    16681 acctgtgaca aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc
    16741 tagtactgcg tcaaagtcac ccgaagttga gcggtcaaga cgcacagaga atggaacagg
    16801 tgttggtttg actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat
    16861 tacttttttc gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc
    16921 caattctttt ttcgcttttt cccaatattc ttttgcctta tctttgtcat aagaaactaa
    16981 ttttccagct tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg
    17041 gatcaatcct gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg
    17101 atcgattgaa taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc
    17161 acgttggttg aattcgatat agcttgtccg tgcttcttta acagaagtat aagctggatc
    17221 gtttgcgttt tgctgtgcta attcaccaga caagatcaca tcatcggctt ggccatcttt
    17281 gaataagttc aacgcagtag aagattcttt tactacactg actttgattt cagaaagttt
    17341 tactgtgtct ttgtcccagt aatggtcatt tttcttataa gtccattctg tatctgttcc
    17401 tggtccatcg aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga
    17461 tgcataggca tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa
    17521 agcaagcagg tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc
    17581 gccaactgct ttgatcccta gttctgattt atctttcttt ccagctgtga tgtcttctgc
    17641 gttttcaact ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg
    17701 ttgccagcca taaacataat ccgctgctgt aacaggatca ccgtttgacc attttgcatc
    17761 ttctcgtaat ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc
    17821 tcctgccggc tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt
    17881 caatgctgta aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg
    17941 aggcacgact aagttaaata cgcctcctcc atctgcagaa ccgctttttg ctgaactact
    18001 gcttgtggct gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa
    18061 accaaatgta aatttctttt tcattttacg taccccttta ttggaatttt caga
//
LOCUS       DADJYG010000047        17992 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.047, whole genome shotgun sequence.
ACCESSION   DADJYG010000047 DADJYG010000000
VERSION     DADJYG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.047"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..796)
                     /locus_tag="JZ382_001263"
     CDS             complement(<1..796)
                     /locus_tag="JZ382_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAY2427460.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIINIIKRI"
     gene            complement(769..1449)
                     /locus_tag="JZ382_001264"
     CDS             complement(769..1449)
                     /locus_tag="JZ382_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAY2427461.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1472..2410)
                     /locus_tag="JZ382_001265"
     CDS             complement(1472..2410)
                     /locus_tag="JZ382_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAY2427462.1"
                     /translation="MKKYLLTATVMIGALLFAACGNMNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDGLKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLEALKESIN"
     gene            complement(2655..3506)
                     /locus_tag="JZ382_001266"
     CDS             complement(2655..3506)
                     /locus_tag="JZ382_001266"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY2427463.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLVEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3660..6233)
                     /gene="mprF"
                     /locus_tag="JZ382_001267"
     CDS             complement(3660..6233)
                     /gene="mprF"
                     /locus_tag="JZ382_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAY2427464.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSHMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLVTVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKSLFSEFYPKGLFGLLLASFGQW
                     TGALAVFLTIGKLMRIDISMFSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFLTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTLNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLAFGILNTITRTLSWQLLLVYVLILLAVWLSRKEFYRTKFVYSWGA
                     ILFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTTLGSEYEKERFDRLLETYGGTATSHQLRLPGYSYFYYQKDDHDQVV
                     FGYQIKGNKCFVLGDPIGNPKMMQAATNQFMKRADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLHGAREKYFSVGRFDESYVLSSGVGIVRKEQKVVGFITAQPMTKEQAGYDLLRILPE
                     EPEELADYLLANLFIVYEKMGYLEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6374..7180
                     /gene="proC"
                     /locus_tag="JZ382_001268"
     CDS             6374..7180
                     /gene="proC"
                     /locus_tag="JZ382_001268"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY2427465.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKTGTVSKEEVFVSSGHFENAQTFA
                     AETGVHACKSNQEVASKSDIVLLAVKPKIIEKVLKELKEYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATIIFEAIGSVYPLAE
                     ADFSTFIGLAGSSPAFIYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7259..8815)
                     /gene="rny"
                     /locus_tag="JZ382_001269"
     CDS             complement(7259..8815)
                     /gene="rny"
                     /locus_tag="JZ382_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAY2427466.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9234..10280)
                     /gene="recA"
                     /locus_tag="JZ382_001270"
     CDS             complement(9234..10280)
                     /gene="recA"
                     /locus_tag="JZ382_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY2427467.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDTKRQEELPLEE"
     gene            complement(10389..11630)
                     /locus_tag="JZ382_001271"
     CDS             complement(10389..11630)
                     /locus_tag="JZ382_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAY2427468.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTKDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGHPVGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNTVMQGFDLLRKQLLRK"
     gene            complement(11801..12379)
                     /gene="pgsA"
                     /locus_tag="JZ382_001272"
     CDS             complement(11801..12379)
                     /gene="pgsA"
                     /locus_tag="JZ382_001272"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY2427469.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQLNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFNLISIP
                     VDQIMLYACLIFTIYSGADYFIKNSGVFKGSM"
     gene            complement(12587..13471)
                     /locus_tag="JZ382_001273"
     CDS             complement(12587..13471)
                     /locus_tag="JZ382_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2427470.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAIHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTQESSTVESTTESSEEKKMAIAAGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWIGVLVDNAYVYQGTLAANETQSTVLPETAANATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(13878..15164)
                     /locus_tag="JZ382_001274"
     CDS             complement(13878..15164)
                     /locus_tag="JZ382_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY2427471.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGQQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEEKNFPPKQEIVRYFPENTKEIIKQSALEAAITRNKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKIKEIILGFADDPEVSEMNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLVAGEAFISEEAFSEFYMRPK"
     gene            complement(15157..16425)
                     /locus_tag="JZ382_001275"
     CDS             complement(15157..16425)
                     /locus_tag="JZ382_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY2427472.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAKLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNQQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPSLGVVEELDKITARSLAAYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFEPRETAHPELFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLRLIHEQL
                     ESLRNGEITDLELAQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            16678..17139
                     /gene="mscL"
                     /locus_tag="JZ382_001276"
     CDS             16678..17139
                     /gene="mscL"
                     /locus_tag="JZ382_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY2427473.1"
                     /translation="MIKEFREFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WIVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRAKEAEEEVEEAPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17197..17643)
                     /locus_tag="JZ382_001277"
     CDS             complement(17197..17643)
                     /locus_tag="JZ382_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427474.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTYFQDTDGKILAYTRVYEEVDSIHFGRVLVHQNNRKDGLGRKIVQETINMIQKKF
                     PEKPIVIGAQEYLQAFYESFGFKATSDVYLEDDIPHIDMKKEQVTS"
     gene            complement(17736..17981)
                     /locus_tag="JZ382_001278"
     CDS             complement(17736..17981)
                     /locus_tag="JZ382_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427475.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5122 a         3931 c         3170 g         5769 t
ORIGIN      
        1 atattcgttt gatgatattg ataatcagga acaaagcaat aaagactaat gtgattgttg
       61 ctcctggagg tgtatctaga taaaatgagc tagttagtcc tgacagcatt cctagaaatc
      121 cgacacaaac actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg
      181 ctgcgggaag aaccatgatt gcggatatca aaagtgcacc ggcaattggg atcatcacag
      241 caatagccac accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat
      301 ccacgtgggc agtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga
      361 acaatatacc gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg
      421 tcacgatcga accaaacaga taagattgga tacttgttgc tgaattccct ccgctcaaat
      481 tcatcaatac taatgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg
      541 aataggttcg gtagatcgtt cgcagatact ctaatatcac agctgctaat acaacgacta
      601 gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag
      661 agacatgaga aagtgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta
      721 gcatcggggc gatccccgct atgaagatag ccgcaagaaa agctcttctc atgaattcat
      781 atgaaagcaa cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata
      841 ctgtttgata tcttcatgat catgagtgat cattagaact gatttcccat ggacatgcgc
      901 actatgtcgg agaagacgat aaaattcatt tctggattct tcatccattc ctgttgttgg
      961 ttcatcaaga acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg
     1021 cttttgtccg ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc
     1081 ttctaaagca cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc
     1141 tcttggatat cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa
     1201 agatgcaact tgttgaggaa tatagccgat acttaatttt tttccttctt gatttttctc
     1261 agcaatcttt atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt
     1321 tgacttggct gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata
     1381 agagacatct tctagtacag gctcatcatc ataataaaag gtcaaatcag atatttcgat
     1441 ataacgcacc atacaacctc cattctaaaa cttaattgat actttctttt aaagcttcca
     1501 gatttttttc catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc
     1561 tttctaaagg attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta
     1621 ccttcgaaga ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt
     1681 cttttagttc agctaagcgg cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg
     1741 ctacttgttt taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa
     1801 atgtgcggtt tgttgcatct tttagcccat cggagtattt ttgatccagt gcttcaagct
     1861 ttttgatata agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc
     1921 ctaattcttc agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat
     1981 gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt
     2041 cttgcgattc taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat
     2101 ccactgaatc ctttaatgaa tccacgaaaa attccatgtc tgaactatta tacacgaaaa
     2161 catctgcatc ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg
     2221 ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc tacgatttct tttgtaaaat
     2281 catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt
     2341 tcatatttcc acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat
     2401 attttttcat ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacga
     2461 gaactaatgt atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa
     2521 acatgtatat tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagtcattt
     2581 cactacaata aaaaactgaa gaataccact ttaggattcc tcagtttttc atcacctatt
     2641 ttacttttta tgttttattg cagggttcgt actaaataaa cttcctcaca aaaacctttt
     2701 aacccattat atactctctg cgctcttgaa taatgttgac acaaggcaaa aacggttggt
     2761 ccacttccac tcattaatgc agcatctgct ccgtacttga tcatacgctc tttgatttga
     2821 tggatgactg gatgccgctt tcccgttata tcttctaaac tatttcccat ttttttgatc
     2881 atctgctgat aatcttgctc tcggatggct tcaacaagtc cgtggatgtc ttgatgttgg
     2941 atggcttgcg tatctacttc tctaaacact gtacgtgtag agacactcat tcttggtttg
     3001 accaacacaa cccagcactg tggcatagaa ggtaatactt ggatttcttc ccctttgcca
     3061 ccgacaaatg ccgtacttcc gtagacacaa taaggaacat ctgtaccgac ttccaaaccg
     3121 atttctgcca gttcttctaa cgaacagccc aaattccata attggttgat ccctcttaga
     3181 gcagctgcac agtctgtact tcctccgcct aatccagctg ctactggaat attcttctta
     3241 acaaagatct tgacaccggt tttgatattg tatttatttt ttaacaataa agcagcttga
     3301 taaacattgt tccgttcatc taccggtaaa aatgctttat ttgtttctac aatgattttg
     3361 tcttctgtta tttcttcaaa aaatagatga tctgccaagt ctacgctcga cattaccatt
     3421 tccaactcat gatacccatc ttgccgtcta tataatacat ccagtcctaa atttatcttg
     3481 gctggcgctt tttcaatcaa ttccatcctc tcacccattc gaaaagtcta agtcgcaagt
     3541 attctatcat agggaaaaat aaaagtatag cttatgtctg cctagtggtt gattttgtca
     3601 tcgtttcaac agctttcata gaattttctc ttcataatcc aaataaactc tagtgattct
     3661 tataattctg tgagcacttc ttctgccaaa ctcggcacat ttcctttttc tttccctatc
     3721 agactgagca gctgcatggt agcaaatccg aaattacttc ctttccgata tgcaaagtag
     3781 cgaccttccc aatgatcgac atattttaat ttcttttcat aagcagactg gaaggaatag
     3841 aaaaaattac cataattata tacgatatgc atgacttttt cttgaaagaa agaaaaatct
     3901 gtttctccaa catttgctaa aggggctaat cctagatttg cttccagata gcccattttc
     3961 tcgtatacaa tgaacaaatt agctaataga taatccgcta attcttcagg ctcttcaggc
     4021 aagattcgca gtaaatcata ccccgcttgc tccttcgtca tcggttgagc ggtgatgaag
     4081 cccacgactt tttgctcttt acggacaata ccgacgccac tagacaatac ataggattca
     4141 tcgaatcgtc ccacagaaaa gtatttttct cgtgcaccgt gaagccattt ttgtgaaact
     4201 tcatttacag cttgatgtaa ttcttctggt agttggtcat accaagtgaa atggtatcct
     4261 tctttcatca aacgttgata ttcaagttgc tgactcagcc gagacaagtc ttctgtcgct
     4321 agatcaacaa ttcctgcttc acctactttc gtgaaatgaa agcccaattc gtgaagcatc
     4381 actacgtagc tttctgatat cttataaaaa gcaagctgat aacctaaaca atctgctcgt
     4441 ttcatgaact gattcgttgc tgcctgcatc attttgggat taccaattgg atctcctaaa
     4501 acaaagcatt tattcccttt gatttgatag ccaaaaacga cttggtcatg gtcgtctttt
     4561 tggtaataaa aataactgta tcctggcaag cgaagctgat ggcttgtagc tgtgcctcca
     4621 tatgtttcca ataaacggtc aaaacgttct ttttcgtact cactgccaag cgtcgtagta
     4681 tcttctgcta agtattgata aagcgccact aaagctagca atgagatgcc caaaccaatg
     4741 agccctgaga accacacatc atctgaagga aacaaaataa aatggttacc tgttatccgt
     4801 ccaactaaaa agcgtccact atgataacct gcaattgcgt aaacaataaa caagaaaccg
     4861 aacaagctag catcaaatag tatagctccc caagaataaa cgaattttgt tcgataaaat
     4921 tcttttctgg ataaccacac agctaataga atcaagacat ataccaataa caattgccaa
     4981 gatagtgtac gtgtgattgt atttaaaata ccaaaagcta gtaaagcaat cgtaggcaga
     5041 aatgcctttt tgacttttac tgaaagcgct cgagcaagac cgagcaataa aaagcccact
     5101 aataggttca atgtctggtc taaaaagttg aacgagaacg gcaataacac ctgaaacagc
     5161 cgactaattg cagacagatt agtgatcgtt gacaggagta ccatcatgat tcctgcaaaa
     5221 tatacagcac ttacgagcac gatatgcgca accttttgga aaaataatcg cggtatatta
     5281 tctaaaaatc gattgatttt gactcctgta tgtgtgatga acaatagaag tcccgtcaaa
     5341 aaaggcagga cataataaaa caaccgataa tataacaacc aaactactgc tacgtcttgc
     5401 cgaacagaca attgactcat tcctaaaatc atcaaaacat caaaagtccc cattccacct
     5461 gggaccattg tcaacattcc tatcagcgtc gcaatcatga acataggata gatcgaaaac
     5521 atggaaatat ctattctcat caacttgccg atagtcagaa ataccgcaag tgcccctgtc
     5581 cattgtccaa aagatgccag tagcagacca aacaagcctt ttggataaaa ctcagagaac
     5641 aaggactttc tctttagata agcaaaaaag aaaatgactg gcgctaaaaa actaccgccg
     5701 aaaagccaga tccaatagct ccgaaaaata ctttctgact gaataaaaaa gacatcaata
     5761 aatgtaagaa acgaccatac agacaaacca gtcagcataa atagcgcgac ttttgaaaca
     5821 gtcactaaca ccattttcct atttgtctgc tttccataaa aactagcccg aagactggct
     5881 cctaccacac caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga
     5941 aggaactctt ttctttccat ttttggtttt ccttgttttt ctaagactct gatcgtcacc
     6001 caatcataca acaacatcgg agtgacaccc agcagtccag ccagaatcat gagcagaatc
     6061 gtcatgtggc tttgttgtcc catcctttgc caaatatctt gccaactcat gccattagca
     6121 atatttgcga cttgatttgc cacaaagact aacacggagc cgaaaaaaat caattttaaa
     6181 atcagactat gttcctttat ccattgcagc atcaagtatc gtttattttt cacattttcc
     6241 cattcctaac ttgtatactt ttgtgttata ccccaatgtt atcttattta ttttaatttt
     6301 tccactttag acctgttctt tgcttgcgat catagaaaaa gaaagatatc ctaatgctaa
     6361 aggagaggat aacatgaaag taggatttat cggtacaggg aatatggcac aagctattat
     6421 tgatggttta ttgaaaacag gaacagtctc gaaagaagaa gtgttcgttt ccagtggtca
     6481 ttttgaaaat gcacaaacat tcgctgctga aactggcgtc catgcgtgca aaagcaacca
     6541 agaagttgca tcaaaatcag atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa
     6601 agtattgaaa gaactaaaag aatacagcca gaaggtctta ttcatttcta ttgcttcagg
     6661 aaaatcatta acagatttag aaacagcgct tgaagatcct caggcctcca tcgtacgtgt
     6721 gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc
     6781 tgccacggag gaacagcttc aacaagccac gataattttt gaagcaattg gtagcgtcta
     6841 tcccttagca gaagcagatt tcagtacatt catcggcctt gctggcagct cgcccgcatt
     6901 catttatatg ctgattgatg cgatgggacg gactggtgta ttaaatggtt tgtccaaaca
     6961 gacggccact gaaattgctg caaaagcaat catgggttct tgtcaaaaat tcctttcaag
     7021 tgataaaaat ccatgggaac ttgtagacca agtttcttct cctggtggta cgaccgttgc
     7081 cggtgtagtt gccctagaag aagcaggatt catcccagca atcatcaaag gaatcaatac
     7141 gacgatcgag aaagatcttg agatgttgaa taaaaaataa taatagcaat gttttttcgt
     7201 accttacaaa aaaagaagag ctgcggcttt tgccgcgact cttcttttgt gtttactctt
     7261 atttagcata atcagttgcg cgagtttccc gaataacgat tactttgata tgtccaggat
     7321 aatccaactc ttcttcgatt cgtttacgga tatcgcgtac taaacgaaca gcgtccagat
     7381 ccgagatttc ttctggtttg accattaccc gaacttcgcg tccagcttga actgcgtaac
     7441 ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa cgacggatgt
     7501 agttttccaa tgattcactt cgcgcaccag gtcttgctgc tgaaagcgcg tctgctgcag
     7561 ctacaaggac agagataaca gaagtcgctt ccacatcgcc atggtgagaa gcgattgcgt
     7621 tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag
     7681 atccttcaat ctcatggtca agtgcttttc caatatcatg aagcaacccg gcacgttttg
     7741 ctaattggat atcttcccca agttctgctg ctaagatacc agctaatttt gcaacttcga
     7801 ccgaatgctt caagacgttt tgtccgtaac tagttctgaa tctcagacgt ccgatgatct
     7861 tgattagatc tggatgaagt gtatgggcac cgacttcaaa tgctgcttct tccccatatt
     7921 cacgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg attcgtgctg
     7981 gatggattcg tccatcttgt atcagttttt ccaaggtcat tcgtgcgatt tctcttcgga
     8041 ttggatcaaa tccggaaaga acaactgctt caggtgtgtc atcgatgatc aaatcgatac
     8101 cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata cgacctttca
     8161 tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc
     8221 agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct tctttggcac
     8281 gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct tcagtcgatt
     8341 tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct agttcttttt
     8401 gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat tgctgttttg
     8461 aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac gagtcatctt
     8521 tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt aattctaact
     8581 tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct tccttagctt
     8641 ctaacaatgc ttcttttttt agagtttctg cttctttctt tgcactatcg atgatcccag
     8701 cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg accataaacc
     8761 ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat accataaaat
     8821 ccacctccat actatctttt tcatttttat catctataca gcttctatga taaattattc
     8881 taaacttgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac aactttattc
     8941 taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt tccgttcatt
     9001 cattaaacgg tggtttttag aataaatgac actagtttca tataagtaaa cctttaagtt
     9061 gagaaacttt ttcactaaat tactttttgt agtcgaatag atatttttta ttttatgtag
     9121 ctactcgtat gattaaataa ctcaagattt ctttgttctt tattcaataa aaaaacggca
     9181 gatcaaggag agtatctact cttcagacca gccgtttttg tttatcatga tttttattct
     9241 tctaaaggca attcttcttg tctttttgta tcttctggga cagccacttc ttcaccaatg
     9301 ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg attagccata
     9361 tagattttag cattttcccg tccttgacca atccgatctt ctttataaga ataccaagca
     9421 ccgcttttat caacgatgtc tttttctact gccatatcta gaagttctcc ttcttgtgag
     9481 atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt
     9541 tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt caattgttct
     9601 gcacgacgta cttctaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg
     9661 atttctggat tcccaaacat tacgcctact ttttcacgga tttggttgat gaaaatagcg
     9721 attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt
     9781 gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa
     9841 gcggcaactg aatcgactac tacgatgtct acagctccac ttgatactaa agcatcagcg
     9901 atctccagac cttgttctcc tgtatcaggc tgtgaaagaa gtagttcatc gatattcaca
     9961 cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt
    10021 ccgccatttt tttgtacttc tgcaatagcg tgtagcgcca ctgttgtttt acctgaactt
    10081 tcaggtccgt atacttcgat aatacgtcca cggggataac cgccgacacc taaagcgaca
    10141 tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat
    10201 ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct
    10261 gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt
    10321 tcattatcct tatcatatcg tttttagtaa taaaaagcaa gaaaaaaccg aacaaatatt
    10381 cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgta
    10441 ttatatctga tagccgcacg gtctctggca aaatgatatt tttctgaaac cacgccatct
    10501 ttagatgaaa tagcgatcca tactgttcct acagggtgac cttctaatgt gtccggtcca
    10561 gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc
    10621 atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc
    10681 tccagaaaac cagcttttgt ttggagcgag taggtaacaa agccgcctgg aaatacttca
    10741 gatacaccag caatatcgcc caatgcggct tggaaggcac cggctgtcaa gctttccgcc
    10801 gctgttacag tctgcttgtt ttctttcagc agttccacta cgacttttgc taacgaatta
    10861 tcatcgccat atccataaaa atattctcct actcgttctt gaatcttttc ttccaatgct
    10921 aaaagagctt gatttcctgc ctgaacatcc tttgttttta ctgttagacg caatgtcact
    10981 tcatttggtt tggcataagg agcgatcgtt ggattaatct gtgtctcgat tagatctttt
    11041 agttcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaatcagt
    11101 ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt
    11161 ttcaactcat taggcggacc gggaagaagg atatatgcat gtgtatctgt ttgataaaaa
    11221 gtccctaaag caagccctgt acgatttggt agaggaaccc caccttcgat gatttgagac
    11281 tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt
    11341 ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt
    11401 gtcagatcat cttctgtcgg ccctaatccg ccacataaga gtatgagttc acttcttgtt
    11461 tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacgac ggtctgataa
    11521 taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg
    11581 acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttcccgc
    11641 tcccttatat agtatacgct gtacttatat atttttttat ttaaaaagag ggcaaggctg
    11701 atccatggac cagcctctcc ccagttcttt tttcattata tgatcaaatt attttcactt
    11761 tatcaagaac cgacgaatct gaccatccac aaaacaagac ctacattgat cctttgaaca
    11821 cgccgctatt tttgatgaag taatccgcac ctgaatagat agtaaaaatc agacaagcat
    11881 agagcatgat ttggtctact ggaatactaa tcagattgaa tgggatatta ttgataaaca
    11941 gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gcagcagcca
    12001 tgacttctcc atgttcgaca agtagtaaac gtaagcctgt gacggctaat tcgcggcaga
    12061 caataatcgc gactacccaa cctggggcct ttccttggtc aaccaacatg ataaaagcgg
    12121 tcataactag cattttatct gccagtgggt ccgcaaattt gccaaagtta gtgacaagac
    12181 ctcgtgcacg ggcaattttt ccgtctaacc agtctgtgat gctggctaat gcaaaaataa
    12241 ctgcgcctac tagctgcgtg acagccagtg tggtgtcccc tacgttaagt tgtccccaat
    12301 ctaacggtaa tcctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta
    12361 atttatttgg taaattcaca ttatcgactc ctttcttccc tattctacgt gactatccgc
    12421 gaagtttcca ccgatattat ttacttttcc aaacgaattt attcaacgga gcatttttat
    12481 tgaaactgat cttttgaaaa taatgaagaa ggaccgcgac acagatttgt cacgatcctt
    12541 cttttttatg ccacaagtaa atagatgaat aaactcattt gtacgatcac tctgcgtatt
    12601 ggagagtcag gttcacattt ttaggactct gattgttttc ccctggatta actggtacgg
    12661 attctccatt cgctttgatc gtcgcattgc ttgctgcacc gatcgtaatg gtcgcattgg
    12721 ccgctgtttc cggtaacacg gtactttgtg tttcatttgc tgccagtgtt ccttgataaa
    12781 cataagcatt gtctactagt acaccgatcc acacacgatt aacagcggta aactcaagag
    12841 tgacaggctt ggtagcatct gtaatattta ctgcgatggc actgccggaa tcctctcctg
    12901 cagctattgc cattttcttt tcttcacttg attctgttgt tgactctact gtagaagact
    12961 cttgggtctg cgtgctttgc tctgaggaag aggactcctc aactgatcct tctactttga
    13021 gcgaagaagc tgtatcgccg attattggat cagcttgacg atcttgccaa gtcatatagc
    13081 ctacgacaac gacgattgcc aaagcgacca atcctaataa aatgacagga agacttgtcc
    13141 aaaaacgact tggatgcgat tcttcttgat gtattgcttt tctggaagta cttactgttt
    13201 caggctgcgg acgttcaggg accgatcctg tcaatttgct ttttccatca aaaacatcga
    13261 ctaaatggtc tccatcttct cctacagctt cagcgtattg cctaataaac gcccgtacgt
    13321 aaaacttacc aggcaattga tcaaaatcat cattgtcgat tgcttccaaa taacgttttt
    13381 gaatttttgt aatctgctgt aattcatcta aagatatctt tttattcagg cgtgcttgcc
    13441 gtaatttttc tccgatggtt tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc
    13501 tccgaagcat ctgctgttgc aacgaaagac gttatgtttg gggctgtgac aaaagtcgtg
    13561 tcacagctcc tttttccgta taaacggcgt tccagaagta gcttcttcgg caataagccg
    13621 aaatttcaca aaaatttgag aagcaatttt cgtcaattcc ttcttatttc ttgaagctga
    13681 ccacttctgt cacaacctct ttgattactt cgtttaattt tattttaata taagttgatt
    13741 aaaagagagc atgatttgtt ttattttctc attaatattt ccaacacatc taagaatacc
    13801 ataaaagctg gttttggagg aggttcttgc tacaagtcta aaacgaaatt taagactttt
    13861 ctccataaaa cactggttta ttttggtctc atataaaact cactgaaggc ctcttcactt
    13921 ataaatgctt ctccagctac cagtacgtct ttcatttgga tcgaatcgat gatttcagga
    13981 agatcaaata atgttcgatc accaaataaa ctttgcgtaa attgattagc aatatattcg
    14041 attgaattca atgattggaa atactgtcca agcattttct tcttcagcaa atcgagattc
    14101 atttctgaca cttcaggatc atcagcgaat ccaagaatga tttctttgat tttttctgct
    14161 gctttctctg gttcatccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
    14221 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatagc taaatagttt
    14281 cgagaagtat tacctaaaat catttggaac aacagattga tagctgtttt ataacgtagt
    14341 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttattt
    14401 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttctgggaaa
    14461 taacgtacaa tttcttgctt tggcgggaaa tttttttctt cttggttttc ccgaataagt
    14521 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
    14581 taaaacgtcc gatagcacgt atacaaatct tgagctgtga ttttgtcgat actttcaacc
    14641 gtgcccgcaa tatcgatatg caacgggtgt gtaggataca agttatttag gataccaaaa
    14701 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccaat gattcctttc
    14761 tccttgttta cagtttcttc tgtaaagtaa ggtgcttgta caaagtcgat cagggtcgtc
    14821 agattctttt ctacctgatc agtcgtagag aacaggtaac tagttttcgt gaaactcgtg
    14881 aaagcattag cggaagcacc ttgctgtcca aataattgaa agacatcacc gtcttctttt
    14941 tcaaacagct tgtgttctaa gaaatgagcg atgccatctg ggactttcac tttttctttt
    15001 tcaccataag gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
    15061 gtcttgtgat agtcgttttt aggtaacaaa tacacggtca aaccgtttgg cagtacttca
    15121 tgataaagcg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
    15181 taaaaagaag attgcttgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
    15241 tacactctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
    15301 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca ggtattggtt
    15361 tcttagcatc gcttttgtct gagctagttc caaatctgtt atttcaccgt ttcgcaaact
    15421 ttctaattgc tcgtgaatca ggcgaagtac ttgatttcgg tttttttcgt cgataccagt
    15481 ctggacactc atgaaccctc ggaatgtatc cacgctgcta gaggcataat aagctagact
    15541 ttctttctct ctcacattca tgaacagttt ggagtgagga aacccgccaa ataaaccgtt
    15601 gaagaccatt agcgcaaaac gttctggctc atcataatac acatttgtct gataagctag
    15661 attcagcttt gcttgaacga tcggctcacg gacttgtcgt tccttgacga tattcacttg
    15721 aggctgagta taaaacaatt ctggatgagc tgtttcacgc ggctcaaatg gtaattgccc
    15781 aacagcttcg gctgctttgt ccggatcaac atcaccgaca acaaaaatgt caatttgatc
    15841 gttcgccatc atttcttgat agtaagccgc tagtgagcga gcagtgatct tgtctagttc
    15901 ttccactacg cctaaacttg ggattttttg ttccgggctg ttttcaaaat aaagttcttg
    15961 cagtgcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat agagacgcag
    16021 attgttcttt tccaagtcaa acgtcaactg atcaaactgt tgatttttga tatttggata
    16081 aaataaaacc tcttttaaaa aatccaccgc ttgagagaaa aggttaggat cattgacata
    16141 ttcgccgttc acaaagctga ttcctgcgtt tagccaatgc aagttccctt tttttcttac
    16201 tgatagtccg aagcttgcac cataaagctc agcaagtttc gcacttaact ttgtctgatc
    16261 cggataattc aaactatttg tttccaacat actgctgagt agtgaacgtt ttgtaatgac
    16321 ttcttgttgc agacgtgccg taaaacggat aaaaatccga atcgttttgt attgttttgt
    16381 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
    16441 ttcatagatg attgcccctt actcttttag taggacaatc ggcctttttt acttatgcat
    16501 ctgcttgaaa tatatataag ttcagacaat tcgccgattt ttctaaatga tgttacactt
    16561 tatagtatag catagaagtt ttttatcaaa agggacagac agctaaaagc gtatatcgtt
    16621 tttttatttt ttatttttcg atatactaaa gacatacaaa agaagggagg gtcattcatg
    16681 ataaaagaat tcagagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggtgtt
    16741 gttatcggga gtgcgtttac aagtatcgta aaccaagtgg tgcaaggatt aatcactccg
    16801 ctaattggtt ggattgtcgc tatgatcaca ggaacttctg atttagaagg agcactttca
    16861 attcttgact ggtcgccgac aaaaggtgtc acatttgcgt ttggtaacgt tattagtgct
    16921 atcattacat tcctgattac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt
    16981 gcgaagaatt tacgcgccaa agaagcagaa gaagaagtgg aagaagcacc tactgcagaa
    17041 gattatctta gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacgcat
    17101 ccggtagagc aaacaaatct ttcagatact tctaaataat gaagaatcta aaaaaagaaa
    17161 gccgattttc agtgtcggct ttcttttttt agattcttaa cttgttacct gttctttttt
    17221 catgtcgata tgcggaatat catcttccaa atacacatct gaggttgcct tgaatccaaa
    17281 agactcataa aaggcctgta gatattcttg tgcaccgatc acaattggtt tttctggaaa
    17341 ttttttctgg atcatgttga tcgtttcttg gacaatcttt cttcctaagc cgtctttacg
    17401 gttattttga tgaacaagaa cgcgaccaaa atggatgctg tctacttctt catatacccg
    17461 cgtgtaggca aggatcttcc catcagtatc ttggaaatac gtatgccaag cttgtccatc
    17521 gatatcatca acttcttgat aagggcactg ttgctccaca acaaagactg caatccgttc
    17581 tttgacgatt gcataaaact ctgctgtcga caattcttca aatttcttta cctgatattc
    17641 cactaagtaa acgcctccta attttttatt attagcatac cgattcatac acaaaaaagc
    17701 aaacgaacct tacgaatctc aggctcgttt gctttttaaa ctgcttcagt taaaaattcg
    17761 atctcaactg ttcgagtaag tacatctgaa tagctgtaag atactcgttc aaaagaattt
    17821 tcttcaggat ccaagtcaac aacgaatact gaaggatagg tttctgttaa aacgccgcgg
    17881 cgttctgttt gacgctttct tcctgtctga gcaaccaaca tgattgggct gccgatacga
    17941 ccttctaaat ctttcttgat tgaagctaaa gttgttggca tacagttcac ct
//
LOCUS       DADJYG010000048        17513 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.048, whole genome shotgun sequence.
ACCESSION   DADJYG010000048 DADJYG010000000
VERSION     DADJYG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.048"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            77..1762
                     /locus_tag="JZ382_001279"
     CDS             77..1762
                     /locus_tag="JZ382_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY2427476.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1776..3059
                     /locus_tag="JZ382_001280"
     CDS             1776..3059
                     /locus_tag="JZ382_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY2427477.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3129..3998
                     /locus_tag="JZ382_001281"
     CDS             3129..3998
                     /locus_tag="JZ382_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427478.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3995..4831
                     /locus_tag="JZ382_001282"
     CDS             3995..4831
                     /locus_tag="JZ382_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427479.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4857..5924
                     /locus_tag="JZ382_001283"
     CDS             4857..5924
                     /locus_tag="JZ382_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY2427480.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5921..6748
                     /locus_tag="JZ382_001284"
     CDS             5921..6748
                     /locus_tag="JZ382_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY2427481.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6793..7818
                     /locus_tag="JZ382_001285"
     CDS             6793..7818
                     /locus_tag="JZ382_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2427482.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7815..9998
                     /locus_tag="JZ382_001286"
     CDS             7815..9998
                     /locus_tag="JZ382_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAY2427483.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            9998..11128
                     /locus_tag="JZ382_001287"
     CDS             9998..11128
                     /locus_tag="JZ382_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAY2427484.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11125..11802
                     /gene="pgmB"
                     /locus_tag="JZ382_001288"
     CDS             11125..11802
                     /gene="pgmB"
                     /locus_tag="JZ382_001288"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY2427485.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11886..12413
                     /gene="msrA"
                     /locus_tag="JZ382_001289"
     CDS             11886..12413
                     /gene="msrA"
                     /locus_tag="JZ382_001289"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY2427486.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12614..13300)
                     /locus_tag="JZ382_001290"
     CDS             complement(12614..13300)
                     /locus_tag="JZ382_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY2427487.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13380..14918
                     /locus_tag="JZ382_001291"
     CDS             13380..14918
                     /locus_tag="JZ382_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY2427488.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15038..15505
                     /locus_tag="JZ382_001292"
     CDS             15038..15505
                     /locus_tag="JZ382_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAY2427489.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16024..16467
                     /gene="rplM"
                     /locus_tag="JZ382_001293"
     CDS             16024..16467
                     /gene="rplM"
                     /locus_tag="JZ382_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY2427490.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16483..16875
                     /gene="rpsI"
                     /locus_tag="JZ382_001294"
     CDS             16483..16875
                     /gene="rpsI"
                     /locus_tag="JZ382_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY2427491.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16963..17502)
                     /locus_tag="JZ382_001295"
     CDS             complement(16963..17502)
                     /locus_tag="JZ382_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2427492.1"
                     /translation="MYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRK
                     DTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLN
                     YRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTP
                     DIVDKAVFEAFQRGLNKCD"
BASE COUNT         5698 a         3224 c         3777 g         4814 t
ORIGIN      
        1 atgctattga cagtaagcgt tttccataat atgatcaaat cgttgggaac gctcccaaaa
       61 aagaaagggg aatccgatgt atacaaaact tttacaagcg atttatcgcg actcagattt
      121 gtctgagaaa accgcctcta tgattgaaca aaaagtcagt gtttatcagc agaagaaagg
      181 ccaggcaggc aaactgcatt gggacgaaga agatattttc ttgattactt atggggatca
      241 attttttgaa gagcagcaac caactttgcg tacatttaag aaattttatc aaaagtattt
      301 ggcagataca tttagagttg tacatttctt gccatttttc ccatatacat ctgatgatgg
      361 tttctccgtt gtaaattatc aggcaatcaa cgaccgtttg ggcacatggg aagatataga
      421 agaaatgaga agcgaaacac ggttaatgtt cgactttgtc tgcaatcata tgtctgccga
      481 aagtacttgg tttcaggaat atttaaactt gaatgatacg tacaaagatt tttttattga
      541 tttgccactg gatacagata caagcagcgt gactagacca aggaccagcc cgttacttac
      601 aacgttcatt gacaaaaacg ggaaagaaaa acacgtatgg acgactttta gcgaagatca
      661 agtagacttg aattttaaaa atccagaagt tttattgaaa atgatcgata ttcttttgtt
      721 ttacttagaa aaaggcgctt catttattcg cttggacgct gtgggatttt tatggaaaga
      781 actaggaact tcttgtatac acctgccaga aacgcataaa atcatccaat tattccgtgc
      841 aatcgctgat gaagcagctc caggaacggt attgatcact gagacaaatg tgccacacaa
      901 agataatatc agttattttg ggaatggcga gaatgaagca cagatggtct atcagttttc
      961 gctaccacct ttgacccttc aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg
     1021 gttaaaaacc atcgatttcg catcgaaaaa aacaactttt tttaattttt tagcctccca
     1081 tgacggtatc ggattgaatc cggtacgagg tattctgccg gaaaaaacga tccttgaatt
     1141 agtggaacag ctgaagaaag agggtgcccg agtaaactac aagaaaaacc cagatggaac
     1201 gaccagtcca tatgagataa acgctacgta tctagatgcg ttaagttctc caacagatac
     1261 gacggaatgg agaagcaaac gctttttagc tgctcattca tttttggtaa cgttaccagg
     1321 cgtgccggca atttatattc aaagtgtgct gggaagtcga aatgattaca aaggcgtaga
     1381 aaaaacagga gagaatcgaa aaatcaatcg gcaaaaattt ttgttagcag atattgagaa
     1441 agagttgcag acagatccat taagaacagc agtatatggt gaattgaccc agatgctatc
     1501 tattcgaaaa aaagaaaaag ccttccatcc gaatagtttg atttacttac tggactcagc
     1561 agattctttt atcagtttta ttcgcgaagc gaaggaagag aaagtcttag ttatccataa
     1621 tttttccaat caatcagcag gttatctttt accaaaagga gattacctag acctgttttc
     1681 tggacaaatt atccattgca gtcaagaacg aaagttgata ttccagccgt atgaatatat
     1741 gtggctgaaa ataataaaat aagaggtgga aaaaaatgaa aaaaattatt gttactacct
     1801 gtctgctagc tactggattt cttcttactg cttgtggtga aagtcaaagc tctaacgaat
     1861 cacaacagac aacgattcaa ttcatgcact cttctgtcga acaggaacga ttaagtgtga
     1921 tcaatgaact ggttgctgat tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc
     1981 cggtagaaga atcagcgttc aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac
     2041 cagaagtaat ggaagtcagt caggattttg ctaaagtgat ggataaagat gaactgattg
     2101 atcagacggc agtccaaaac gttatcgagg aagctggaga agataattat tatgacggag
     2161 ccaaaaacct tgttcgttca gaagatggaa aaagctatat tgctgcacca attagcggct
     2221 gggttcaagg tatttggtat gacaaagaaa aattatcaga agctggtttt tcagaaccag
     2281 aaaattggga tgatatttta aaaattgctc aacatttcac agataaagag aataaacaat
     2341 atggaattgc tatgcctact gctgaaggaa caatgagtga gcaagcgttc tctcaatttg
     2401 ctttgtcaaa tggagcaaat gttttggatg atgaaggcga ggtaacgatc gatacggaaa
     2461 agatgagaga agcattgtcg ttttatcaaa acttatccca atacacgatg ccaggctcta
     2521 atgatgtgac agaaatcaaa gatgctttca tgaatggaac agcaccaatg gcaatctatt
     2581 caacttacat tttgccttct gtttatgaag cggggaactc agaaaatatc ggttttgcta
     2641 tcccaacaca aaaagatcaa gctgtctatg gcacagtatc tggattgacg atttcttctg
     2701 gtctagacga gaaacaaaaa gaggctgctg aagcattcac agctttctta tctcaaccga
     2761 aaaacatgga aaagtgggtc ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag
     2821 tagtcgaact ggatggttac aaagaaaatg aagtcatcaa gtcttttggc gagctgccat
     2881 ctgagattgc tagtgctttt gatgaagtac aagtctttgg actagtcggg gaaaagaatt
     2941 tcacgaaaat gggagatatc acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa
     3001 ctgtcggaaa cgaagatgtc gatcaagctt tagctgacgc acaaaaaaat acgaaataac
     3061 aagaagttct gccaatggtg ttcgaaaaaa gtagatagct attggcagat tatcaggggg
     3121 aggtttcaat gaatcgtcca aggaaacatt ctgacttaaa attagccatg ggacttttag
     3181 caccaagcat gcttttatta gctgttttag tgctgattcc aatgctttcc aatattcaga
     3241 tcagtttttt cgatatgccg ctgaatccta actcagacgc cgtttttatc ggattaagca
     3301 attatcttga aattttaagt gatagtgaat tttatcgttc tttaggttta acagttgctt
     3361 acacattctt agttgtagca ggcagtacag ctttaggatt agccgtagct attttgctta
     3421 accgcaagtt cagatttagg aaaacggtgc gttcactgat tttattaagt tatgttacgc
     3481 catctatttc tttgattttt gcttggaaat atatgttcaa taatagttat ggaatcgtca
     3541 atgatctgtt agtagatagg ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc
     3601 cagttgctag cttctttatg gtcgttttgt ttgctatctg gagatactat ccgtatgcgt
     3661 ttatttcatt tttggctatt ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa
     3721 tggacggtgc aaatgcttgg cagaagttca agatcgtgac actgccggca atcaaaccag
     3781 tactagcaac tgtgattgtt ttacgatcta tctggatgtt ctatatgttt acagatgtct
     3841 atctactgac gaataaagtc aatatcttag gcgtttattt gtatcagaca gcctttgcgt
     3901 tcaatgatct tggaaaagca gcagctatat cgattctatt gttcatcatt attgcagcgg
     3961 tcgtcttgct ctcacgaaag aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat
     4021 cttattttac gcgacgattt tgttgctatt agctgtcaat ttgtttccat ttttgatcat
     4081 gctgatgaca tcattcaagg attcacaaga agcaattgca acgacacaaa ctatttttcc
     4141 gaaagaatgg acttttgaac attatcagga tattttcaat gctgagattt ttccattcat
     4201 catgtatttc aaaaatagtt tagtgatctc cttgatagcc tccgtaactg cagtagtgat
     4261 tgggatcagc ggggcatacg ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa
     4321 tgctagcttt tacaccgttt atatgttttc cggtattttg atcgttgttc cattatttaa
     4381 aatcatttcc ggtctaggtt tgtatgatac caatacagca ctgattatca cgatggtcgt
     4441 tcaaacactt cctactgcga tattcatgct aaaaagttat tttgacacga ttccgactga
     4501 tattgaagaa gcagcaatga tcgatggtct tagccggatc cagatcatta ttcggatcat
     4561 tataccattg gctatttctg gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg
     4621 gaatgattat ttatttgctt cgattttcct gtcgacctcc gaaaaattta ctttaccaat
     4681 cggtttgaac acattattca gtacaccaga ctacatttgg ggacgaatga tggccgcttc
     4741 attggtgacc gctttaccag tcatcattat gtatgcgtta tctgaacgat ttattaaagg
     4801 tgatttagtc gaaggcggcg taaaaggata aaaagaaaag aaagaggaaa gaaaagatga
     4861 aaaaattagt tgcattagaa ccaagaatag ccggattcaa agattacgaa gaccgtgaga
     4921 tcgaaaagaa cgaagtacgt atccgagtaa ttttcgctac tccaaaacca ggaacagaaa
     4981 ttgtcgactt tagaggatta agtccattta tcgatgaaga attttcagaa gaatggcgcc
     5041 tattcttgcc aagagaagaa ggaagccaac gcggtgtggt atttggcgaa tttcaattag
     5101 gaaatatgat tgtcggtacg atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag
     5161 gagagttagt ctgcacttat ggacccattc gtgaaacgct gattacaaac ggtgttgata
     5221 attataaact acgtaaactt gatcggttgg atcaatggaa aaatgcagta tgttacgatc
     5281 ctgctcaatt cgctttaggt ggtatccggg aagcacatgt tcgagcaggt gatgctgtag
     5341 caatcttcgg tttaggcgct atcgggcaaa ttgcgattca attagctaaa aaagcaggtg
     5401 ctactaccat cgttgggatc gatcccatag cgcagcgtag agaattagca gaaaaacacg
     5461 gtgccgatta cacgctagat cctatgaaag cagatgtcgg ttttcgtcta aaggaattga
     5521 cagataaacg tggagtagat tcagttattg aaacgagtgg cagtgctgcc gcattacaag
     5581 cttctttaat agggatcggc tatggcggga tcatttctta tgttgccttt gctaaacctt
     5641 ttccagctgg gtttaatctt ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg
     5701 ctcgagcatc tagtgaacca aacccagatt acccaagatg gaaccgtaaa cgaatcgagg
     5761 acacatgttg ggaattattg atgaacggtt acttggattg tgaagaaatc attgatccag
     5821 ttgtgccatt tgcagaaagc gcagaagctt acatgcgtta tgtagaccag cacccagaag
     5881 aaagtatcaa aatgggcgtt caagtccgag gaggagaaca atgaaattcg gtacgcaaga
     5941 tcaagcattt ttcccaagag acattttaga aaaatttaga tttatcaaag actgtggatt
     6001 tgaagaatac gaaatcgacg gtaagttgtt aatagaaaat atcgacacag tcaaacaggc
     6061 aatcaaagaa acagggatcc cagtagtgac agcttgtaac ggctacgatg ggtggatcgg
     6121 tgactttatc gaagaacggc gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc
     6181 actaagcgag gttggaggca aaggaatcgt tatcccagca gcttggggaa tgttcactta
     6241 tcgattaccg ccgatggttt caccccgaag caaagaagga gactataaag cagtcagcga
     6301 atcacttcgt tatttagata aagtagccga agaaacaggt acggttattt atttggagcc
     6361 tttgaaccga taccaagacc atatgatcaa tttgttagcg gatgcgcgtc attacattga
     6421 ctctaatcaa ttgaaacatg tgcagatcat cgctgatttt tatcatatga atattgaaga
     6481 agatgatatt tcaaaagcat tgactacgca aaaagagtat gtgggtcata tccatttagc
     6541 agataatcat cgctaccaac caggatctgg cgcaattgat tttactcgcc attttcaaac
     6601 gctacttcaa aatgagtatg aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa
     6661 tgaagtgaaa atttatcagg aatcattgca atacttgaaa aactgtctgg aaacagcgcg
     6721 tcatttaaat gatttagaga aagaatagag aaagaataac aataagtaaa taagcaaagg
     6781 agagagaaat aaatgaagaa agtagcaatc ataggcgctg gacaagtagc agaaaaagta
     6841 catgctgctt attatcaaaa aagatcagat gtcttagaat tggccgctgt ggttgatcca
     6901 gtcagccagc gagcagaaga cttttgccga cgtacaggat tttctcatgc gtatacatca
     6961 gttacacaaa tgctgaatga gatttctccc gatatcgtga gtgtctgtac acccaatcgc
     7021 tttcacttcg aacatgtatt ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa
     7081 ccaccagcta tgacagctga tcaagcaaga aagatggcag agacagccga taaaaacggg
     7141 cagattttgg cttatggatt ccagcatcgt ttttctgacg aagcaacaat gatcaagaat
     7201 catcttgatg ttctcgggga tatttattac gtagaagcga atgcgcttcg tcggtctggt
     7261 gttccaggct ggggaacgtt tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat
     7321 tatggcattc acatgcttga tacagcgatg tatattactg gatttcctaa agtaaaaagc
     7381 gttcgggcgt acagcttcca aaaaattggt acgaaaaaaa attcagggaa attcgggaaa
     7441 tgggatccaa gcaaattcac agtagaagat gcaatttttg gcacactgga attagaaaac
     7501 ggcaagatca tccaactgaa tacttcgttt gctttgaata tcagagaaga caaactgaat
     7561 gtggcttttt gtggcgatca ggcaggtgct aatctgtatc caggcgaact attcgaagat
     7621 gtagatggaa agctggtttc cttacaaaag accacgccag ttaatgaccc acatgaacta
     7681 agtatggctc attttgtgaa tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc
     7741 tggcaaggat atgtcatcca gaatctggtg gaaaatttat atcgttcggc agaaacagga
     7801 gaagttatta ccttatgaaa gtagaagaaa aacagtttga tttagagtat atcaataagt
     7861 atgcgagtca gatgaccatt ggaaatggat tttttggtat cagaggaagc caggaggaag
     7921 actatcggaa tcaagtaagg ggaatgtttg cagctggtgt atataaccga cctttaggag
     7981 cagaatcagc cgagttagtg aatctcccag atgtcatcag atgggaaata aaaatcgacg
     8041 gtgaacattt ttctcttgag acgatgaaag ttcttgcata cgagcgggca ctagatttag
     8101 aaatgggaga gcttaaacgc tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag
     8161 tcattacttg tcgtgttgct gatcaaaata atctccatca ggcagccctg aaagtggtca
     8221 tcaagccgtt gaccgaagac tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa
     8281 ccaattttgg tacacagcag ttggttgaaa aagaactgcg agtatttggt gacgatttga
     8341 tcagtgcatc ctaccagaca acagaaagta aaattgatat tgggattgca gctaaattca
     8401 ataaagcagg tgtattccaa gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga
     8461 tcagaaaaaa tacgtccttt acgctggaga agatcatcgt gctctctacc tctcttcagc
     8521 aaacagaccc gtttaagaaa agtattgccc aagcccagaa aagcgttagt tatacagaaa
     8581 ttttagagca gtcgatgtct tattggacag atttttggca tcagcatcgg ataacgatcc
     8641 aaggcgattc ttctttcgat cagttagcga tggacttcgc tgcttaccat ttacagatta
     8701 tgactccccg caatgatcca agtgttagtg taggggcgaa gggactaact ggcgaaggtt
     8761 ataaagggca tgttttctgg gatacggaaa tttttatcct gcctttcttt ttatatcagt
     8821 ttcctgaagt agcaaagaat ttgctgctgt accgttatga tcgattagaa ggcgcacggc
     8881 aaaaagcaag agattatcac tatcaaggtg cacttttccc atgggaaagc gcacacactg
     8941 gtcaggaaga gacacccaaa tttgctgcat tgaatatcaa aaccggaaca agacaagaag
     9001 ttgcttcagc tgccgcagaa catcatatca gtgcagacct tgcttatgcc gttcaagagt
     9061 atgtccaagc aacagaggat caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg
     9121 aaacagcagc tttttggctg agcagagctg taaaagtcga tgatcattta gagatacaca
     9181 gagtcattgg tccagatgaa tacacagaat atatcgacaa taatgcatat acgaactatc
     9241 tggcgcatta taatgtggcc ttagctgtta aatatcaagc tggcgatccg ctttttttga
     9301 aagaatgtca aaatttttta gataagttat atttgccagt gcctaacaaa gaacagctga
     9361 ttccgcaaga tgacacgtat ctcagtaagc cagttattga tttgaccact tacaaagaac
     9421 gtcctggcag ccagtctatt ttacttgatt atgcccgtag cgaagtaaat gaactccaag
     9481 tcagcaagca agcagatctt gtcatgctgt tatacttatt acctgagtat ttttctcaac
     9541 caatcgtttc taagaatttg cattactatg aaaacaaaac aatccacgac tcatcgttga
     9601 gtaaagcgat tcacgccata gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt
     9661 tattccaaga agcgtgcctg attgatttag gaccagatcc gcattcatca gatgacggaa
     9721 ttcatgctgc ttcattagga gcactatggc tagcagttat atttggtttt tcaggtatta
     9781 cagtaacgaa tggaactctt tctatttctc caagacttcc ttcagcatgg gaaaaaattt
     9841 cttttccttt tatttggaaa aatcacaggt tgctatttgt tttgacaaaa aaacaaatag
     9901 taataaaaaa agagacgaaa gaaacattac ctatttttgt caatggagac caataccagc
     9961 tggaaaaaga actgatcttg gaacggaggg gaaaataatg cgtattctga ttgcgccaga
    10021 ttctttcaaa gaaagtctga cagccttaga ggcagcacaa gccattcaat caggttttca
    10081 agaagttttt gttcaggctg aatatgatct gctacctata ggcgatggag gagaaggaac
    10141 ggtttcatca ttagcagaca gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc
    10201 atttggcaaa aacaaagaaa tccagtacgc ttcagacggt caaactgcga tttttgaaat
    10261 ggcagaaatc gtcggattga aagatgtccc actaaaccaa caacagccac ttaggttatc
    10321 cacgcgagga gtaggagaac tgatacgaca attgttgcaa caaggtgttc gacacatcat
    10381 catcggcgtt ggcggaagta gtaccaacga tggcggcatc ggcatggcag aaggtctagg
    10441 ttatgctttt tacgatcaag ataatcaaaa agtagaagca ctgggagaaa atctaggtaa
    10501 aattgctaca gttgataaaa cgaacgtgcc tattgagctg attaaagcca aaatcactgt
    10561 cattactgat gttactaatc ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc
    10621 acaaaaaggc ttgaaagaaa atgaattagc agtggctgac caagccatgc atgattttta
    10681 tatgagattt gcccctcaag taatcgaggc atcgggcagt agtgctggtg gtggaatggg
    10741 tgcaggactg ttagcatttg cttcagggaa aatagtctct ggcatcgact acgtattgga
    10801 tttactggag ttcgataaac gggtacagca agcagacctc gtaatcgtag gcgaaggacg
    10861 tctagatgga caaagtctaa aaggaaaagc gccagttggg attgctagaa gagtaccaga
    10921 gggcgttcct gtgatagcta tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga
    10981 tgttggcatt tcggccgttt ttccgatcct tcctgcagct gtgcctttac aagaagcttt
    11041 acagcaagcc actgataatc ttcatcgcac agctaaaaac atcgcatctt tacttcaagt
    11101 cgttcatttt caaggaggta atcgatgaaa atcaaagcag cactattcga tttagatggc
    11161 gttcttgtag acacagctcg ttatcattac gaagcgtggc tggttttagc taaccagctg
    11221 tccattccat tcactgagaa agaaaacgag caactgaaag gaatatccag aaccgagtca
    11281 ctagagcgat tgctgtcatt tggaaaaatg gaacagaaat tctcagaaaa agagaaatca
    11341 gcctttgctg agcaaaaaaa caatctatat cttcaagcga ttcaaaaaat ggatgaaaca
    11401 tccgttttgc ctggtgcaat agctgttctt gagtacttga aaaaaacaaa catcaagatc
    11461 ggattgggat cagcaagcaa aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt
    11521 tattttgatg tccttattga cggcacgcaa gtatcaaaag caaaacctga tccagaagtt
    11581 tttctcaaag gagcacagca attgaatgta ccgccaaatg catgcttggt cattgaagat
    11641 tcagaagctg gttgtcaagc agctttggct ggtaacatgc atgtattggg aataggtgaa
    11701 aacataaatc ttccttctgc tgaatatgtg attcctgatt taaccgtttt tgatcaagta
    11761 cgttcgtttt ggcatttatc agaagccgta tcacgtatat aatagtttcc gttttttctg
    11821 tcacaagcaa aacgttatcc tccttttgga aatcaaggta tgatgagaaa aaagggggat
    11881 tttatatgac aaaaaaagca gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat
    11941 ttgaagagca gccagggatc atttctgtga catccggtta tactggcggg catgtaccaa
    12001 atccaactta tgaacaagta acaacaggca caacagggca tacagaagca gtagagatcg
    12061 aatatgatcc agaagttatt tcttatgatc agttggtgga aatctattgg cagcaaaccg
    12121 atccgactga cgcattagga cagttcgctg atcgcggtga ttcttatcgt ccagtgatct
    12181 actataatga tgaagaagaa cgaaatattg cagagcgttc aaaacaagca ttgcaagaca
    12241 gcgggcgatt cgataagcca atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg
    12301 cagaagatta ccatcaggat tattacaaga aaaacaagct ccattacaat ctttatcgag
    12361 aaggttctgg acgtgcaggt ttccttcgtc gacattgggg cgtcaagaaa taagaagccg
    12421 aaaaagctac ttatggaaca ccgtatatca gaggttgtaa aaccagcatt ttgagctaat
    12481 actgagggtc agctgattga acaccattta gtggagaggt tgtgaacctg tcgcctagct
    12541 ccaagtcaca aggatttggt aagagaacac tatatatcag aggttgtgac aagtcgtttg
    12601 tcacaacctt ttttcaatac ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag
    12661 gaatgaaagg cagcttttcc aacttttttt tatgacagat aaagtaaaca gaaaaaacgg
    12721 atattccaaa cagactagca agaaacaaga tctgaagcaa ctcttctaga gacaagccac
    12781 cactccaaga aagcatcaac agagtatcgc cagctccgag ataggattga ccatagcgaa
    12841 ggcacacacc gcctacgata aacgttaaca gcacactttg ccattggaag acgccggcct
    12901 gcaaccaaaa aatccataaa attgcccaag aacagaatag cgtgtgagaa tggaccatga
    12961 agtaaaataa atccatcaac gaaaatagca aagccgaaag catccatatg aacgtcgaaa
    13021 aatttcttgc ggaagaaaaa gtaaagaacc aggcaaaaag accaccacat aaggcttctg
    13081 ccaacaaata gtagaagggc aaccgacacc tgcaatagcg gcagcgaaat cgttggacga
    13141 tgatcgagag aatcggaatc aattcatacc aagaaagcga acgacagcat ttgacacaat
    13201 gagatctagg ataaataata gagtgaccta acggaatccg ctgggcggtt aaacataaaa
    13261 atgatccgat acagcaacca atgataaaat aaagtaacac gccatctccc ccttttcaaa
    13321 accaagatac gcaagcttaa actaaatttt tgtacataca taaaatttga ggtgaaaaaa
    13381 tgaacgtttc aatcggatta gatatcggaa ccacacaaac caaagcagtt gctttcacag
    13441 atgaagccca agaagcagca tccgcttatt ttcgctaccc tttgatccaa gaaacacaag
    13501 ggatggcaga acaagatcag gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag
    13561 taattaaaca gttgccttca gacacaatca ttcaatttgt ctccttctcc tctgcgatgc
    13621 atggcctgat attgttggat gaagagaaaa caccgttatc tcgaatgatc acttgggcag
    13681 ataaccgagc agcggacgaa gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt
    13741 atcggcagac tgggacgccg attcatccaa tgacaccatt gaacaaaatc aagtggctgc
    13801 aaaacagcca acctgaatta ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata
    13861 tcttttatag attgtttggc gaactgatca gcgactacgg aacagcttca ggaactggat
    13921 attttgatat ccatgaattc caatgggcag aagaagtgct tgactacttg aacattcgaa
    13981 tagagcaatt gccgcaagtc tttccttcca cctatcaagt aacaggtcta ccatcaaaaa
    14041 cagcagcaga actaggtttg ccaaatgata tatcatttgt tttgggaggg gcagacgggc
    14101 cgctttctaa tctagggtta ggtgcttttg gtgatacgat cgccgctttg accgtaggaa
    14161 cgagcggagc acttcgtttt attactactc agcctcagtt ccatccagaa atggagacgt
    14221 tttgttatgt tctggatcag aaacattggg tagtgggagg agcgactagc aatggtgctg
    14281 gcatctttga ttgggcaggg catacatttt tacaggaagt cgttcaatct gctaaagaaa
    14341 aaggagaaaa tccatatgat gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca
    14401 atggtctgat ttttcatccc tatttacttg gtgagcgagc accgctttgg gacgcagatg
    14461 cgtttggcag ttttatcggt ttacagcggc aacatacaga aaaagaaatg atgaaggcga
    14521 ttctagaagg gatctgtctc aacctttatc ggattttaaa aggagtttgc caggaaaatc
    14581 agctcacaga gatccgtgct acaggcgggt ttgctcaatc tccgattttt cgtcagatga
    14641 tggctgatgt actgggttac ccgcttgttt tccctactgt cacagaagca tccgcattag
    14701 gagctgtgat tttaggctgg agaagcatcg gaaagatcca aagtatcagt gaagcacagg
    14761 aattgataga attgaaagag caagttggag tacaagaaac tgatcataaa aaatatcaga
    14821 aaatctatcc attatttgtt tcgacacaaa agcatttagc acagacttat cagcaatttg
    14881 ctcaactgcg agaagaatta gctgattctg aaaaatgatt ggctttacgg ataaaaaagc
    14941 gaactgaaaa tcgttctcag gtaaatgatt ttgttgtact tttcattgaa agtctactat
    15001 tattttaagt gtaagaaaat gttaggaggc aagaaagatg aaattccaac aaaccaaaga
    15061 agtattaaat caattagttg cagatctaag ccaaatgtct gttgttgttc accaaacaca
    15121 ttggtacatg agaggaccag gtttccttac attgcaccca atgatggatg agttcatgga
    15181 tgatttgaac gctcagttag atgaagtatc cgaacgcttg atcacattag acggttcacc
    15241 ttattctaca ttgcgtgaat ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg
    15301 ggatcgtact attccagaac gtttggaaac acttgttgca ggctatcgtt atctagcaga
    15361 tctttaccaa aaaggaatcg aagtttctgg agaagaaggc gacgatccaa cacaagatat
    15421 ctttatcgaa ttcaaaacag ccacagaaaa acgtatctgg atgatccaag ctcatttggg
    15481 caaagcgcca gagatcgacg cgtaaaaact gttgttgaac ttgaccgaaa aagaggttgt
    15541 gaaatcagcg ttttgacctg agtattagag aaacaaacgt caaaatagtt cctcatattt
    15601 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttac caagaggttg
    15661 taagcctgcc gttcagctcc gagtcacaag aaacaatttt aaaaaacagc ttcttatgtt
    15721 ttatggatat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt attcaaaata
    15781 aggggttgtg acaagacttt tgtcacagcc tctttttgtt tttcattttg acaggaaacg
    15841 acccttttat ataaaagaaa gaaaagaagt aaagtttcat ataaattttt caatcaagca
    15901 gaaaaacgtt gacattgact agattagcgg gtaatatgtt aaatggtatt gtttgcccca
    15961 caaagagccc cggaaactcg aagtgtatgt caaacatcag aacttaaatt ggaggaaaat
    16021 aacgtgcgta caacatatat ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta
    16081 gacgcaactg atgttccatt gggacgtctt tcaacagttg ttgcatctgt actacgtggt
    16141 aaaaataaac caactttcac acctcatttg gatactggcg atttcgtcat cgtcatcaat
    16201 gcagacaaag tgaaattaac aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc
    16261 aactacccag gaggattgaa atcaatcact gctggcgaat tgcgtgctaa aaattctcgt
    16321 cgtttgatcg aaacttctgt aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa
    16381 tttacaaaat tgaacgtata cggtggagca gaacatccac atgcagcaca acaaccagaa
    16441 gttttagaca tcacaaactt aatctaatag gagggaattt cattggcaca agttcaatat
    16501 atcggcacag gccgtcgtaa aaacgcagtt gctcgcgtac gtctagtacc aggaactggc
    16561 aaaattactg taaataaaaa agatgttgaa gaatacattc cacatgctga cttgcgtgaa
    16621 gtaatcaatc aacctttcgc tgttactgaa acaaaaggcg catatgacgt atttgttaac
    16681 gtaaacggcg gaggatacgc tggacaatca ggagctatcc gtcatggtat cgctcgtgca
    16741 ttgctagaag ttgatcctga cttccgttta gctcttaaac gtgctggctt acttactcgt
    16801 gacgcacgta tggtcgaacg taaaaaacca ggtcttaaga aagcacgtaa agcttcacaa
    16861 ttctcaaaac gttaatatcc tttatatatc aacgtttctg gcacttttca attatttgga
    16921 aagtgcttct tttgttttta agaacacatt taagaacaca tatcaatcac acttattcaa
    16981 tcctctctga aaagcttcaa aaacagcctt atcaactata tctggtgtgt tcacatatat
    17041 ctttgttgta gaaatatctg agtgtgttaa agcatttgaa atttgattca tatctgctcc
    17101 accttgtcta gctatagttg cataagtatg acggagtttg tgtggagata aatgaactaa
    17161 gtcaggatgc cttcttttaa ttgaattgat tcgatagttt aggtaatcgg catgaacagg
    17221 acaatttact tctccagatg gtttagtata agtaaagaga tactgatcta acgtttgctt
    17281 aatttttaat ttcaacaatt gatcagcttg taccgatttc cattcactta gcaatgtcat
    17341 tacaacttct ggtacttgaa tgacggtatc ttttcgacct tttgtaaact tttttctttg
    17401 atatttatct aaagtatgtt taagacggac cgtttgtttt tcaaaatcaa tatatttcca
    17461 ttgcaaagcg taagtctcac tttttctatc accaagatac agggtaatca taa
//
LOCUS       DADJYG010000049        17345 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.049, whole genome shotgun sequence.
ACCESSION   DADJYG010000049 DADJYG010000000
VERSION     DADJYG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.049"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            2..369
                     /locus_tag="JZ382_001296"
                     /pseudo
     CDS             2..369
                     /locus_tag="JZ382_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            549..1490
                     /locus_tag="JZ382_001297"
     CDS             549..1490
                     /locus_tag="JZ382_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY2427493.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="JZ382_001298"
     CDS             1559..2290
                     /locus_tag="JZ382_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAY2427494.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="JZ382_001299"
     CDS             2434..4614
                     /locus_tag="JZ382_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY2427495.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="JZ382_001300"
     CDS             4634..5116
                     /locus_tag="JZ382_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAY2427496.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="JZ382_001301"
     CDS             5132..5893
                     /locus_tag="JZ382_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY2427497.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="JZ382_001302"
     CDS             6024..7358
                     /locus_tag="JZ382_001302"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY2427498.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7786..8271
                     /locus_tag="JZ382_001303"
     CDS             7786..8271
                     /locus_tag="JZ382_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAY2427499.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8264..8947
                     /locus_tag="JZ382_001304"
     CDS             8264..8947
                     /locus_tag="JZ382_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY2427500.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9073..11718
                     /gene="polA"
                     /locus_tag="JZ382_001305"
     CDS             9073..11718
                     /gene="polA"
                     /locus_tag="JZ382_001305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY2427501.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11763..12599
                     /gene="mutM"
                     /locus_tag="JZ382_001306"
     CDS             11763..12599
                     /gene="mutM"
                     /locus_tag="JZ382_001306"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAY2427502.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12563..13192
                     /locus_tag="JZ382_001307"
     CDS             12563..13192
                     /locus_tag="JZ382_001307"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY2427503.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13244..13576
                     /locus_tag="JZ382_001308"
     CDS             13244..13576
                     /locus_tag="JZ382_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY2427504.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13888..14379
                     /gene="nrdR"
                     /locus_tag="JZ382_001309"
     CDS             13888..14379
                     /gene="nrdR"
                     /locus_tag="JZ382_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAY2427505.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14402..15775
                     /locus_tag="JZ382_001310"
     CDS             14402..15775
                     /locus_tag="JZ382_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAY2427506.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15775..16701
                     /gene="dnaI"
                     /locus_tag="JZ382_001311"
     CDS             15775..16701
                     /gene="dnaI"
                     /locus_tag="JZ382_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAY2427507.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16830..17270
                     /locus_tag="JZ382_001312"
     CDS             16830..17270
                     /locus_tag="JZ382_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY2427508.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3821 g         4584 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttcccgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt tttcttggct gttcttatta cttgatgcag ggcagctttt tcacgacctc
     7561 agataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa tgaaaaatat
     7621 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7681 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7741 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7801 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7861 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7921 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7981 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     8041 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     8101 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8161 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8221 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8281 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8341 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8401 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8461 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8521 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8581 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8641 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8701 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8761 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8821 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8881 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8941 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     9001 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     9061 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9121 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9181 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9241 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9301 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9361 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9421 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9481 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9541 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9601 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9661 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9721 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9781 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9841 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9901 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9961 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
    10021 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
    10081 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10141 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10201 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10261 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10321 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10381 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10441 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagataa aaaccaagaa
    10501 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10561 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10621 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10681 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10741 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10801 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10861 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10921 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10981 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    11041 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    11101 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11161 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11221 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11281 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11341 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11401 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11461 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11521 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11581 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11641 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11701 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11761 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11821 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11881 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11941 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    12001 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    12061 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12121 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12181 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12241 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12301 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12361 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12421 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12481 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12541 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12601 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12661 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12721 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12781 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12841 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12901 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12961 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    13021 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    13081 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13141 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13201 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13261 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13321 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13381 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13441 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13501 tgaccacatt tccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13561 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13621 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13681 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13741 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13801 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13861 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13921 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13981 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    14041 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    14101 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14161 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14221 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14281 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14341 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14401 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14461 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14521 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14581 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14641 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14701 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14761 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14821 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14881 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14941 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    15001 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    15061 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15121 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15181 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15241 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15301 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15361 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15421 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15481 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15541 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15601 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15661 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15721 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15781 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15841 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15901 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15961 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    16021 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    16081 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16141 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16201 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16261 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16321 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16381 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16441 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16501 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16561 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16621 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16681 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16741 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16801 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16861 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16921 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16981 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    17041 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    17101 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17161 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17221 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17281 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17341 atggg
//
LOCUS       DADJYG010000050        16853 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.050, whole genome shotgun sequence.
ACCESSION   DADJYG010000050 DADJYG010000000
VERSION     DADJYG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.050"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(364..3069)
                     /locus_tag="JZ382_001313"
     CDS             complement(364..3069)
                     /locus_tag="JZ382_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAY2427509.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIRSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3375..4322)
                     /locus_tag="JZ382_001314"
     CDS             complement(3375..4322)
                     /locus_tag="JZ382_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAY2427510.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4512..4781
                     /gene="rpsN"
                     /locus_tag="JZ382_001315"
     CDS             4512..4781
                     /gene="rpsN"
                     /locus_tag="JZ382_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY2427511.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(4957..5319)
                     /locus_tag="JZ382_001316"
     CDS             complement(4957..5319)
                     /locus_tag="JZ382_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY2427512.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5330..6451)
                     /locus_tag="JZ382_001317"
     CDS             complement(5330..6451)
                     /locus_tag="JZ382_001317"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY2427513.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6467..6817)
                     /locus_tag="JZ382_001318"
     CDS             complement(6467..6817)
                     /locus_tag="JZ382_001318"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY2427514.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(6947..8458)
                     /locus_tag="JZ382_001319"
     CDS             complement(6947..8458)
                     /locus_tag="JZ382_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY2427515.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8780..10132)
                     /locus_tag="JZ382_001320"
     CDS             complement(8780..10132)
                     /locus_tag="JZ382_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY2427516.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10285..11361)
                     /locus_tag="JZ382_001321"
     CDS             complement(10285..11361)
                     /locus_tag="JZ382_001321"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY2427517.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11557..13302
                     /locus_tag="JZ382_001322"
     CDS             11557..13302
                     /locus_tag="JZ382_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAY2427518.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13582..14019)
                     /locus_tag="JZ382_001323"
     CDS             complement(13582..14019)
                     /locus_tag="JZ382_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY2427519.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14003..15376)
                     /locus_tag="JZ382_001324"
     CDS             complement(14003..15376)
                     /locus_tag="JZ382_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427520.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15698..16186)
                     /locus_tag="JZ382_001325"
     CDS             complement(15698..16186)
                     /locus_tag="JZ382_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427521.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16208..16810)
                     /locus_tag="JZ382_001326"
     CDS             complement(16208..16810)
                     /locus_tag="JZ382_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY2427522.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4735 a         3610 c         3062 g         5446 t
ORIGIN      
        1 tagaaaatca agttctatcc tttttgatat cttaactttt ggaagagaga tgtaaggctg
       61 ctttctaccc atcattctta ctactcgaag catagcgcag ttgtcacaac ctcttgataa
      121 acggtgttta aaagcttgca tctgcggtaa taagcccaac caaattaaaa acataagaag
      181 ctgtttttat ttggtcgttc ttattacttg atgcagagca gcttttttac aacctcttgg
      241 tacacggtgt tcaatcagct gaccctcggt attagctcaa aaaaacggca aaatatgagg
      301 aactattttt ccattttttt ctctaatact caggtcaaaa cactgatttc acaacctctt
      361 tttttaatga aaaatatggc tctcatgcgc cgcttctttg aatttcgtca ttttttcttc
      421 gatgaacgga tagattgccg ttcctacgat tccaaagaca acaaacaaac cgatcatgat
      481 ccagattgct ttcgtagcat ttggccaata aattcctcct gcagattccc gtaacaagtt
      541 taccgcatga gtaaatggca agaacggatt gatcatttgg aagaatttcc ctgagacttg
      601 gatcggatag ttcccgcctc cgccagagat cgacagtact aagatgataa tagcgatccc
      661 tttaccgaca ttatcgaata atgcagccaa gacatagacg atcatcatga acgctaaggc
      721 gatcaagaca gcaaatgaca cgctgtagac cggattttgg acatctactc ctagtaagaa
      781 aatattccct aatgtaacga tcaagctttg cgcaatcgcc attgtcaaaa atgtcagcat
      841 acgagcaacg aattgttctc gtttcgtaaa attgtttcgg tctttctttc ctaaatagta
      901 ttctgtcgta gcaacacttg aaagaagaac agcgccgacc cacagacata gtgcagtata
      961 aaacggggtg ctagctgaac cgttatttgc gattggataa agcgcattgg tttgtagctc
     1021 aacaggttgt gtaaagaagt cactttcttt tgttgcgtct agtttcaata atttgatgac
     1081 ttcgcctaga tcgacttctt gttcgccttt tcggatcgcg tctgctgctt tttggatacc
     1141 agctttgata tttggccagt cattgttgat caagtccgca gcagcgttca cagctgtttc
     1201 gacttctggc attttttcat ttactgttgt caatgtaccg gtgatttcat tttttagtgc
     1261 tggccaatca ttttggataa aatcagcggc caatcctaat ttttgctgaa tgatcggtaa
     1321 ttcattgttg tataggtctg ccccttcatt gatgccattg acgatggtat ccatatttcc
     1381 attcagcatt gtattggcat catggatttc ttgtccaatt gcaggcattt ctttttgata
     1441 tttttctaaa attgccactg cattcgttac agttgctttc gttgagctga gaagcgaatc
     1501 aaagtcgatc tggtccgctt tgctcagtgc gccttgtgca gtcccaatcg tatcgatgac
     1561 ttggtctagt agagaagtga cagtggaact gatttgacct gtattgatac cagcaatgat
     1621 gccattgata ttattaacaa cggcatcaat ttgatctagc aattcatcaa tagccttcgt
     1681 gtcttcattt tgaacagctg tgttcaaagc agataggcgg ttttgcagat tgcttagcaa
     1741 gggttttaat tgatttagct gatcaataat tggctgtaat ttcgtatttc cagcacttgt
     1801 ttgcaattta gtcaggaact cgaccagttt atcgattgca ttttgttgtt gagatattcc
     1861 gtcaataaag ccgttcaata cattgctgat gtttttcttt tcatcatcag tcagcttacc
     1921 atcttcaata gctgttctta cagtatctat taacgaacct gtcgttgttg ccacttgtcc
     1981 aattgacttc aatgtaacat ctacactgtt tgtgatgctt ggtatcgcat ctttcagctg
     2041 agaggcacct gattttgttt ggtttgctag atcacttgcc tgattttcta aatttttcac
     2101 atctggcaag atatcttgta cttgttggat gatcgtcaag ccttgttttg cttcatcgat
     2161 tccttggttc attgtctctt ggatcgaagc aaaatcgccg tcgatctctg ctagttgctt
     2221 accggcattt tggatctctg ggattttttc ttgcaaatca aggatgattt ttgcttgatc
     2281 tttcaactca ggcattttgt cattcaatgc aacgactttt tcacccatct catctacttt
     2341 agggatgtaa tccacgaatt catttgcttt attcagtttt tccttgattt caggtagttg
     2401 actttgtaat tcgaggactt gttttgtgta gccgtcgatc gtgtccaaat tttcatctgt
     2461 tgataaaatc atatctttaa ctttattgat acttactagg ttcgtatcga tgtcgtaacc
     2521 tatctcattg aatactttca ataaagtcga actggctgtt ttgataaatt catttgtgat
     2581 ttgcgattgg atcgaacttg ctcctttatc ggtgattttc ggtgcaatcg cattgatttt
     2641 ttcatttacc gtatattcga tttttggttt cttgatatca ccactagtaa aactcagcag
     2701 atcttctgag aaatcctttg gcaagtaaat acctgcgtaa tactttccgg aacggactcc
     2761 gtcttctaat tgttcttttg aatcaacgaa ttgccatcct aattgtttat ttttatgaag
     2821 cgattcgatg acttgctccc caatggccac ttctttacct tgaaactctg ctggcttgtc
     2881 tgcactgtaa acagcaattg gcagctctcc agtatttcca tatggatccc ataatgcttt
     2941 gatgttaaac cacgcatata gcgaacggat gatcatgatc gcgatgatca gaagtgttgc
     3001 aatcgggttt ttgaaaatcc gtttccagtc aagcaaatat aatcgaagcg tatttttgat
     3061 tgactgcata gttctccccc ttcttctttt ttcagctgaa ttactttacc atgtatttca
     3121 tacttttttc aatcggcttt tttcaaaaaa gtcgaatttt tggacatatc acggcaattg
     3181 tctcattgta atctcttaca tattgagaaa taattaagga ttacagcctt tactttatca
     3241 tgaaataaaa gggaaagata attttatgcc aaagttttta tctattatac gtatacgcaa
     3301 aaaagctaaa acattttttg tctttgcccc tacaaaaatg tttcagcttt tttcgttttt
     3361 tattcctgta tttcttattt ttcgaatgac aggatgattt tcccagcagg aggatgtcct
     3421 tcaactagct gatgtgcttg gatgacatgc ttaagtgaag cagacagatt cattgaaata
     3481 aaaacatgaa aggctccttt ttggtaggct ttgattatgc cagcaaaggc ttctgcatct
     3541 agatattcct ttcttggaac aaacgattca taaaagcctt cctttttttg ccgcaaatct
     3601 agatctggga ggtcatttaa tgcaacatac ctgccgcctg gtttcatgat ctggatgccg
     3661 gtttctcctt tgatacttcc tttcgtcgca tcgattacaa ggtctgcctg atcagcaaat
     3721 tgcagacctg gatttgtttt atcataagca gcaaatgctg aagctcctaa tttttttacc
     3781 ttttcctcat ttttactgga tgcactggtt aagattcgga ttcccttttc atggagaagc
     3841 tggatcagac ttgacccgac cgcacctgat gctcctagta tcattacagt atctgtcggc
     3901 tggatttccg tcaaatggtt gattagatta taagccgtaa ttcctggcgt gaccattccg
     3961 gctgcttctt cccaactcat tttatctgga atttttgcta cttttgctga tggcaacacg
     4021 acttcttctc cgtaagtccc gccgactgca tggacagcta ctcgatcacc tacatgaaca
     4081 gttgtgacat ctgaaccaat ctctgtcaca ataccagcgc catcattacc tggtacataa
     4141 gggaatttca atgttcgtat ctctttcatt tctcctaaac gcaaggcaac atcataagga
     4201 ttcacactga atgcttttat ttctacacga acatgtccag aaccgacttc tctgggatga
     4261 gcatcgattt cttcaaaaac atcttgtgct tctccgtaac cgttaattgc aaatcttcgc
     4321 atggtcacca tctccctttt tctttaagtt tacgtttatg cctttatttt gacaaaaaaa
     4381 atgtcttttc gttttggcgc atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt
     4441 gttaaattat ttatctaaaa gcgtaaagat tacgatttac gctaataaaa aataattgga
     4501 ggaatttgtt gatggctaaa aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga
     4561 ttgaacgcta tgctgcttta cgccaggaat taaaagcgca aaaagattac gaaggcttgc
     4621 gtaaacttcc tttagatgct cgtccagaac gattgaaaaa tcgtgattta atcgatggac
     4681 gccctcgcgg atacatgcgt aaattcggga tgtcccgtat aaaattccgt gaattagcac
     4741 ataaaggttt gattccagga gtaaaaaaag ccagctggta atccaagtct ggctgtaatg
     4801 taaaggagga gggcgcatgg accactcctt ggatcatcaa aaaagatacg ttcgcttctt
     4861 ctagcagaaa tgctggtact agaagcagac gtatcttttt ttacgcacag ctggtccttt
     4921 tttatatgta aaggctgcat ggttacttcg ctatatttat ttagtaacag gagtcagtgc
     4981 ttcgataccc acactaattt tcaatgctcg gtcaacttct gccataatgg gtcgatccaa
     5041 atggcagacc ttttctttca atcttgattt atcgatcgtt ctgatctgct ccaacaggat
     5101 aacggagtct ctttctattc ctgttccagt agctttgatc ccaatgtgag tcggaagctt
     5161 aggtttagcc atttttgccg ttatggccgc aacaatgatc gtcgggctaa aatggttacc
     5221 tagattattt tgaataacca aaactgggcg tgttccgcct tgctccgatc ccacgactgg
     5281 agaaagatca gcaaaataaa tatctcctct ttttaccata ggatcctcct tattcttgat
     5341 attctcttgg tatccgctgt cctaatccgc aagccacttc atagtggatc gtacctaatt
     5401 gatctgctat atcctgcata gtgatttctt tgtctgcatt tttgccgata agcgtcactt
     5461 ttgttccgac aggaaattct tgaggcaatc ggatcatcag ttgatccata catacacgcc
     5521 caatcgtttc acagtagttc ccttctacca atagagaaaa accctgcatc tttcgtggcc
     5581 agccatctgc ataaccgatc ggtatcgttc cgatccactc tgcttcagga gtgatatatg
     5641 tttctccata gccgattcct tcacctgctg ggagtttttt gacttgaatc aattcagaaa
     5701 ccagctcaag tgctggctgc aatggataaa cttccggtag tgcgtgccca gagggattaa
     5761 gtccgtacat tgctactcca taacgaatca tattgccgat tgtttcatca tgccataaag
     5821 ccgttgcact attgctgaca tgtacatatc gaggaagttc gggaagtttt ttcagcactt
     5881 ctataaagcg ttctttttgc agattccaat atgtatcatc tgcctgatct gctgtagaaa
     5941 aatgggtaaa taaaccttcc cattctaata cacgactttc tttgatgaat gcagctgcct
     6001 cttttacttc ttcaggtgta caaaatccga tacgacccat cccagtgtct gcttttagat
     6061 gaatcgaaag aggcgtctct agcttacttt tctctaaaac agcagctgct gcttgcaacc
     6121 attcccgtgt acctgcagtt actgataaat catgagcaac agctacagtc agtgcgtcgt
     6181 aaggaaccat attcaagata aggatgggct gagtaaatcc agcttctctc aactcgactc
     6241 cttcatctaa atttgaaaca caaaagcccg aagctccacc tgcaactgct gcctcagctg
     6301 tttcaatagc tccatgaccg tatccgttcg cttttactac tgcaaacaat tcttttcctt
     6361 gcggaagtct tgcaacttca tttgctacat tttcagttat agctttttta tgtatcactg
     6421 ctttcgttgg gcggtgccaa gcgacaacca tcgtttttcc ttctttctag tcttccaaaa
     6481 taatttgtgc aaaagctgtt tcatttgtat gagtaatact gacccataca ttgccttcat
     6541 gaggtgaacg cgtcacaaca ggctgaccat tttcattttt taaaatctcg acatcttgaa
     6601 aggtaacttt tccgatccca gttccccaag ctttagagaa agcctctttg caagcgtaac
     6661 gcccgcctaa aaattctact tggcgatgaa aaggcaagga ctggaatagt ttcatttcat
     6721 cagaggtcaa gattcgtgca atgaattttg gtttttcttc gatcagccga gtgatcctag
     6781 gaagttcgac tgcatcgatc ccaattcctt ttatcatatg cttttctctt ttcaataaat
     6841 ttagaacact ctcattctac caaattataa ccattttgcc acaaataagt ccacaaataa
     6901 caaaaatgaa agtttctctt aaaaaagaga aactttcatt ttatgtttaa tcgttatttg
     6961 aacggattac aaatccgcgt tttttatcat tgtgacgttt accgccatct ttttggctgt
     7021 tattattttt gctataaccg ccttttgatt tgtttccacg ataattaccg ccgtcacgac
     7081 gattacggtt gtttccgcca ccacgtttgc catttttatt gtatcctttt ttctgcattg
     7141 gcaatgggcg ttctggtgtg atcttcactg gcactgcatc agctggatct tttgctgtcg
     7201 tttttagcaa aagtgcaact aaatcttgtg cagaatattc ttctaataat ttatctgctg
     7261 tttgcaggta tttatctaga ccattttctg caagttttgt ttcgatttgt tcaactgcag
     7321 cacctaattg acctttgaat gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt
     7381 ttttcgtcaa gttttcaatc acatgcaagt agtccatttc atttggtgta acgaaagtca
     7441 cagacatacc gccttttccg gcacggcctg tacgtccgat acggtgaacg tagctttctg
     7501 gatcttgagg gatatcatag ttgtacacat gagtgacacc tgaaatatct aatccacgtg
     7561 cagcaacgtc tgtcgctaca aggatatcta aatgaccgct cttaaatgaa cgcaatacgc
     7621 tcattcgttt ttgttgagac aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt
     7681 ctaatccgcg tgccaattcg tctacacggc gttttgtacg tccgaagacg attgttaatt
     7741 caggtgtttg aacatcaaac aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc
     7801 ggacatagta ttgatcgatc aagtcagctg tcatttcttt cgctttgatc ttcacatgat
     7861 gtggattttt catgaattta acgccaatat tcttgatagc tggcggcatc gttgctgaga
     7921 aaagtaatgt ttgtctttga tcaggaactt gtgaaatgat tttttcgata tcttccaaga
     7981 agcccatatt caacatttca tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt
     8041 tcaatgtgtg gcggttaatg tggtcaagca tacgtcctgg tgtacccaca acgatatgtg
     8101 gacgatcttt caagccacgg atctgacggc caatatctgc tccgccgtaa acggcttgta
     8161 cacgtacttt tttgtcgcgc cctaaacgat ataattcttc ttgtgtttgg atagctaatt
     8221 cacgtgttgg tgcgataact aaaccttgta attcgtgacg atctggatcg attttttcta
     8281 acattggtaa tccaaatgca gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt
     8341 cttttccagc taatgctagt ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac
     8401 cagcgcgttc gactgatttc aacaattctg gtgataattc tagttcttta aatttcaaat
     8461 gaatcctcct atatggttgt taactgagga tgtagctttt ttgctacccc gtcgatttgt
     8521 tcggtcaaag tgtattgttt ctttttgaca gtggccacgc acgtttttat ccattcttag
     8581 cccctgtggc accaccgaaa aatcactttg cgaagtttag gcattagaaa agccaaacgg
     8641 gtcgcgttca gctttatcat tattatgaca tctggcttca acaagttgtt tctatggcaa
     8701 aaccagtcca atttctcttc atctttcggc aataggttat tctaacatag tttttcgttt
     8761 tctgcaagtt tacagatttt tatttcattt gctgaagttg ttctaccact tcaaataagc
     8821 ccatgctgtt gctgcctttt agcataatac tatctgttgc ttcaagattt tgtttgatag
     8881 catcaatcaa tttattcttt tctttttcaa aatagaacac aggtagttgg gggtattttt
     8941 ggttcaattc ttcttttagt gcatgcattt gttccccata aaggaaaatc atggcgaatt
     9001 tctcaggatc gatatgttca ctcatttgcg catgcatatt taaagactct ttccctaatt
     9061 ccagcatatc tgctaacaca gcgattctgc gtccttttgt tggcagattt ttaaaagtat
     9121 ctaataccag tcccatggct gttggatttg cattgtagac atcgctcaaa atagctgcac
     9181 catttcctgc cgttagccat tcggttctgt tttgagtgat agaaacatgg gcgagccctt
     9241 ggcgtatctc aggtatggac agtccaaaga agcgcccgat cgtatacgca atcaaagcat
     9301 tcgttacatt ataacttcca ggcagcggga tcgtaaatgt ttctccttct actacaaaat
     9361 cggtttgtgc ttttcctttt gctgtcactt ccgcatgaac atcgccattt ccaataccga
     9421 atgttgtgac tgtttgtact aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg
     9481 gcacaacgag taatccgtct gatacaagtc cgtcagtgat ttccattttg gctttagcga
     9541 ttccttctcg cgacccaaga ttttctatgt gtgcttcacc gatcattgtg attgctgcag
     9601 cttttggttg agccataagt gaaagttccg tgatttcatt ggcgtgatcc atgcccattt
     9661 ccaaaacgat cttttctgta tcttggggca tgtgcaaaat cgtgtaagga agaccaatat
     9721 tattgttgta atttccttgc gtcttatatg ttttgtattt ttcagacaag acagacgcgg
     9781 ttagatcttt cgtggtggtt ttaccattgc ttcctgtaat tgcaacgact tctgcgccta
     9841 ctttttccaa gtaataggtt gccaattgct gcatggcttt caaaggatct gctacaggaa
     9901 taactggaaa attttttgga gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc
     9961 cgtttgtgat cgcttgctgg ataaattcat gtccatctcg cgtaccagca agaggaacga
    10021 ataaacttcc ttcggttatc ttccggctgt caaattcgac attggtaatc tttgtcagtg
    10081 cagagcattc gctgcccacg gcttgtgcaa tttcttgtat tgttagattc attgagatct
    10141 ctcctaaaaa aggttgtgaa aaaagcgttt ggcctaaacg gcgggttcgc atgactctca
    10201 cttccaagta aatttctcta ttcgtattta gaagctagaa tgaacgcggt tacgcaatca
    10261 ctccagcctc ttctttattt ctctttattc atttttttca aaaaaagatt gacgctgatg
    10321 gtatcgattc attcctaact ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat
    10381 attttcccat aagagtgggt acatactgaa ctccgtaaat cctggcatag agtttaattc
    10441 attcaggaat aattcatttt tatttgtcaa aaagaaatcg caccggctca atccgcttcc
    10501 acctaacatc gtgtaagcta acttcgcgta ctcttgcgct ttttgataaa cttcttctgg
    10561 cacttcggct ggaatctgca tttcgatttt attattgata tattttgctt cataatcata
    10621 gaatgctacg tcttttacga cttcaccagg caaagtcgtc cgaacgtctt catttcctaa
    10681 tacagcaact tcgatttcgc gcgcttcaat tccttgttca acgattgctc gagaatcata
    10741 ctgataggct gttgctaaag cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc
    10801 gacactagaa cccatattcg ccggtttgac aaacatcgga taaagcaaag aaccttcaca
    10861 ttgatcaaat acttttttag gattttcttt ccattgattc ttaagtactg gtacataagg
    10921 aacttgcggc acaccagcag cttgtaaaat atacttggtc atgattttat ccatggcaca
    10981 tgcactggtc aatacgcctg cgccgacata aggcatattc aatgtctcta agaagccttg
    11041 gatcgttcca tcttccccgt ttggcccatg taaaactgga aaaacgatgg ctccttcttc
    11101 tttgatttcg cccggattga tcacttttcc tgtaaagcct tcctctgtct gtccacttgg
    11161 gtcccatgaa agatgcaaga catctttgct ggcaggtttt tctgttagta atggaccttt
    11221 gacccattgt ccttcttttg taataaatac gagttgaact tggtaataat tataataaat
    11281 ggcatttaaa actgaaaatg cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc
    11341 atatagtaaa gtaatcttca aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta
    11401 ctctcatatt ttagcacaaa acaaaaataa acgaataact tatttataaa aaaacgagcc
    11461 agcttctaaa agaacgaacg tcatttattt tttgtcagct agattatttg tcccgttttt
    11521 tataaaaagt tatgataaag aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc
    11581 cgcttggcag aaatcattcg agtgttcatc aattacaatg tagtgccaaa ctttgtacag
    11641 caaaaaaatc ccgaacaggt aaaaaaagcc tttgaagaac taggccccac cttcatcaag
    11701 ataggacaga tgctttctgt tcgagaagat ttgctttctt ctgccttcac tcaaacattc
    11761 aaaacattac aagatagtgt acctagcgac acattttcaa cggtcaaaaa gacgatcgaa
    11821 acagaacttt ctctttcatt atcagatata ttcgatgatt tttcaaaatc gccttttgcc
    11881 tctgcttcta tggggcaggc tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt
    11941 aagatccaac accctaacat cgccgaagag atccgacttg atcttcaact atttgaacga
    12001 gcgatccctt tgatcaaata cattcctgaa actagcgtag ttgatctaaa aggggtactc
    12061 caagaagtaa aacgttcctt gatcaatgaa atggacttct taaaagaaag tcaaaatggc
    12121 gaacaattct atcaaaagaa caatggctgg aaagagatcc gcagccccaa gatttatgac
    12181 gctttttgtt cgaaaaaagt gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat
    12241 ttaatgaacg ccgaaaacaa aactgaaact tttatcactg gaattcaaaa caagcagctg
    12301 aaacaagaag ttgcaaagct tttagttgag aactttatga aacaggtgtt tgatgatggt
    12361 tttttccatg ctgatcctca tccagggaat ctgctattcc atgtactaac aaaagaagaa
    12421 cagacacaag ctagccgaaa aactgaaaca gtccatgaaa aagagttcgg ttcctttgct
    12481 tttcgagcct ccacttctgc agaagaccct gttgcgccat ataccatcaa ctacatcgat
    12541 tttggtatga tggggcatct ttctgcagga cttaggcaaa aactcacaca agccgtttta
    12601 gcgctgtata cgaaagatgc ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa
    12661 gaaggctcgt ttgatgaaag tcggttccac caagaactga caagttttct tgaacagtat
    12721 tatgattctc ctattgatga aatcaacttg caagaagtat tcacacatgt cgtgacgatt
    12781 tgtcatcaaa ataatctgca gttcgatcgt gatatcacgc ttttgctgaa agcatttggg
    12841 acattagaag gggtgatccg cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg
    12901 ccttttgctc aacattattt tctcactcat ctggatgtag aagatacatt gaaacaaagc
    12961 ggattagact tattggaagg gatgaaggct gcgcctaaga tccctcaaca gctccaccat
    13021 ctattagaaa tgtggacttc gggacaagga aaagtcaatt tggaattgaa aaagcaagac
    13081 aaattattga gcaggatcga aagtatgatc aatcggcttg ttttcggtat gattttagct
    13141 gcattgatcg tagggtcctc cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc
    13201 gtttccttat tagggatatt tacgtatgct attgccgcat tcgtcattat ttttttggca
    13261 atcgatgcat tgatccaaat gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa
    13321 agaatcagct tcaagaaata agaaggggtt ttacataaat tgtttctcaa atttttgtga
    13381 aaattcggct tatttttcga agaggctact tctgaaacac cgagaataaa aaatgtgaaa
    13441 aagctgttct gcacccagta ataagaacaa tcaaaaaggg aacatggaac acatttatcc
    13501 tgtttccctg ttcccttttt gatgtattcg agcataaata aaataccttc gttacctact
    13561 atcttctgta cttggtaaac cttagtcaat tgcctttttt aacgcttgac gaaaagacat
    13621 atgatctttc tccgtaaagg gttttggacc ctttgtcctt atgccagctt ttcttaactt
    13681 tccactatga taacgttgtt ctagaagcgt gccgatattc gcttcgtcaa attcttttcc
    13741 agagtgatgg aacactcgca cacccttcgg caatacaagt gaagctaaac cataatcttg
    13801 cgtgatcaaa aagtcattct tcttaaccat accgacgatt ttataatccg cactatcagc
    13861 tccgcggtct acgtagacga tttggacatg attagcgtat tctttggtcg tataatggtc
    13921 cacactggtc acgataacca cgtcaagacc ataggattct cctaatttta tgacatcttc
    13981 ttttactgga gaaccatctc catcaatgaa tagcctcatt ctttttcgcc ttcttccata
    14041 aatgataaac agttactaat ccgctcagta aaagaccaac attgatccat tttgccaaga
    14101 taagcagttg tacactttct atcggcactg attcatggtt cagcgtgatc aataatagat
    14161 ggattggctc tcccatttct tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt
    14221 ggatcacact ccctttattt tgagtagtat acttgacaac gatccggcga tccattggaa
    14281 tagctgtatc agtttgataa tattccttat ttagccagct gcctgttact acgtgccaga
    14341 aactaagcag aaagacacct atcgcaaaac catttggaat caagaggacc aataaagaag
    14401 aactcagtcc aagcaattgc atagccaatg gagaccatgt taaacgtttc aaaatccatg
    14461 gtccgactat ttttgctgca atcaagcctg ctgcataagg caaatacata aaaatcgaaa
    14521 gtttttctaa tccgtacagt atgccaacat aaaaactgct aaataaaaaa aggaaattcc
    14581 ctagcattcc atttagaaaa gtaaacagat ttagccagct tggcaacttc cattccttct
    14641 tctggtgtcc taaaacaaac acgaacaaga tgaacagcag caagaaacca taaatagcaa
    14701 gatcaaaaag cctttcatcg aataacaatc gggcatttct caaaagaaac aataagccaa
    14761 agaatatcaa aaataaaaca agctctcgcg tagctaccag atttcttttg atgtctttga
    14821 aatccagttc gtaacgcgga taatgactaa ccgtatggta ggccatgacg taaaaaagtg
    14881 tataaatagt aaagcttgct gcaatctgag tcggtgcagg caactcaatc gcttgataca
    14941 ggagaaatag ccagatagct gcaaataagt actttttccc atccatattg ataaaacctt
    15001 gcgtcttttc gtgaaaattg accgtggtat tagcaggtac aagccatgct gcactcagcc
    15061 ctagcaaaac acttgaaaaa taaaataaag gaaaataaaa tacgcctaat attccaagca
    15121 atgaaccagc accaccaatc agcagagcga tcatcagcaa cgtgtaacta tctagaccaa
    15181 atcgtagact gttgactaaa aatagtccag tcatccgaaa ggcataaaac agcgtaaatg
    15241 gcaaaatcgt cagccaatta tgcgagtctg ttagtcccaa aaataaaaga taaggaataa
    15301 aaataaccgt gttacttaag ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt
    15361 tgagtttttc gaacatgaca atggctcctt aaacagtctt ataactaatc tgctcttcga
    15421 ttttagcatt aaaggggaaa taaaccaatt ttacagaata tattcttgta aaaaaaaata
    15481 cagaaagaag cataagcttc tttctgcatt tttacggtac ttgattactc aagcagacaa
    15541 gcccttctcc acaaaagtct tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa
    15601 atcaattatc ctgtgcctcc aaaatcaatt aggagctctg taaaccagag tccttctcga
    15661 cccgtcgcat aaatttactt taacacagtt cctatgttta tgcgagagct tttttcattt
    15721 tataatcgac catttgaaat ccttgaccga tatctgaaat aagttcttct gtaatctcaa
    15781 atccttcatg cttataaacc tcgattgctt gatgattccc ctgattcact cgcaaaaaga
    15841 tctctctttc ctttgtttgc gcagcaagtt cttcatacca gcgaaaaact gaagaagtca
    15901 atccttgacc acgtacttta tttagcaaat ataatttact taaataaatt tttccattta
    15961 tctcttcata agcagtgtaa ccgacagtct tttcttcgct ttttaataaa aagtaggaca
    16021 caccttctgt cagttctttt tgaatctgtt tttttgactg ataagttttc agcatgtaat
    16081 ccacttgttt ttgaccaatg atcggcacaa aaacttccgg ccatacttct tgtatgactg
    16141 ccatcagctc atccagttca ttttctgtta cttttatttt ttccatacac ccacgtcctt
    16201 ttttacatta taaatgacct tgcgcttgat ggaataaaaa gagcatgatc atgctataca
    16261 cacccaccga ccacggtttc aagaaaatca caatctgcat aaattcccta aaagataatt
    16321 tcgtcaaacc agctactaga cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa
    16381 caactagtac ttttttaata tctttcgttt ttcttgtcag caattcatca aatttctgat
    16441 acttttttgg cgataaaata agctggacaa aggctacgcc atagtaacgg acaagcaaaa
    16501 atccgattgc ttccccaatc actaggccaa tacaactata taaaagccca aatccattgc
    16561 caaacatcag catgcccgct actgaggaga tccctcccgg caaaatagga atgatcactt
    16621 ggatcacttg caacagaaca aatatcatca ctgcatactt gccaaattgc tggataaacg
    16681 cttgcaaaga cgtgctcgat cgaaaaatcc catgatgata agcataaaga atcaacacaa
    16741 agaaaaaaaa cagaccaata atgggcatga tctgaagaca tttttgtaaa actgatcctt
    16801 ttttagacat ctatattccc cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DADJYG010000051        16679 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.051, whole genome shotgun sequence.
ACCESSION   DADJYG010000051 DADJYG010000000
VERSION     DADJYG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.051"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..714)
                     /locus_tag="JZ382_001327"
     CDS             complement(1..714)
                     /locus_tag="JZ382_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2427523.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            889..2313
                     /locus_tag="JZ382_001328"
     CDS             889..2313
                     /locus_tag="JZ382_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2427524.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2612..3319
                     /locus_tag="JZ382_001329"
     CDS             2612..3319
                     /locus_tag="JZ382_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY2427525.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3306..3629
                     /locus_tag="JZ382_001330"
     CDS             3306..3629
                     /locus_tag="JZ382_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAY2427526.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3718..4755)
                     /locus_tag="JZ382_001331"
     CDS             complement(3718..4755)
                     /locus_tag="JZ382_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY2427527.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5076..6059)
                     /locus_tag="JZ382_001332"
     CDS             complement(5076..6059)
                     /locus_tag="JZ382_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAY2427528.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6474..7244)
                     /locus_tag="JZ382_001333"
     CDS             complement(6474..7244)
                     /locus_tag="JZ382_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427529.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7237..8172)
                     /locus_tag="JZ382_001334"
     CDS             complement(7237..8172)
                     /locus_tag="JZ382_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427530.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8488..10137)
                     /locus_tag="JZ382_001335"
     CDS             complement(8488..10137)
                     /locus_tag="JZ382_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY2427531.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10254..12857)
                     /locus_tag="JZ382_001336"
     CDS             complement(10254..12857)
                     /locus_tag="JZ382_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAY2427532.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12869..13918)
                     /locus_tag="JZ382_001337"
     CDS             complement(12869..13918)
                     /locus_tag="JZ382_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY2427533.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13944..14258)
                     /locus_tag="JZ382_001338"
     CDS             complement(13944..14258)
                     /locus_tag="JZ382_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAY2427534.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14274..14720)
                     /locus_tag="JZ382_001339"
     CDS             complement(14274..14720)
                     /locus_tag="JZ382_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY2427535.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14721..16643)
                     /locus_tag="JZ382_001340"
     CDS             complement(14721..16643)
                     /locus_tag="JZ382_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAY2427536.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4941 a         3397 c         2750 g         5591 t
ORIGIN      
        1 ttatttttca acaaaaacag cttcaaaaac aaagtcttga tataaatgtc tcgataccga
       61 atactcgaaa ggtttaccag acgatagagc aacaactttg tctatctcaa tcataaattc
      121 acctggatct atttttaata tttttgcgtc aaattcattc gcagtatctc ctttgatacg
      181 tatgtgcgaa gtctttattt ccagacccag ttcatttgtg atataagcat atacagattt
      241 ctttaaatgt tttggttcca agcctggtat gactgctaat ggcatgaatg tttgctcaat
      301 cgagtatggt tgacaatcaa taaaacggct tcgtacaata ttatatacat agtcttttac
      361 actgatactt aatttatcag ctactttttt cgatggaagt tcaattgaga aagtatggat
      421 ttctgaatct acgtgttctt caccaaccac gctaaacaaa ccttcgtttg gaccaagaac
      481 tcgtgagtta tcaattggtg ttctgacgac ttcagtccca tacccacttc tacttcgcaa
      541 aactccttcg gaaacaagta agtctacgcc tttctttact gttaacttgc ttgtatgata
      601 aataactgcc aattcatctc ctgtaggaag tttctcacca attttatact ctccatttac
      661 aatttttttc tttatatcta agtaaatttc catatacttc gcaatttttt tcaatggtga
      721 ccctccaatc aaaaacatat cttcatctat actttaacat aaataaaccg ataaaatcca
      781 catcaaaatt atacacacaa taaaaaagat atatatattt aaacaaaact atttacttta
      841 agttgtaagc ggtgttatca ttaagtggaa acaggaggta aaaaaattat gacaactatg
      901 aaaaagctaa cttttccaga tgatttttgg tggggttccg cttggtcagc tgagcaagca
      961 gaaggaaaag gagaaacagg aaaagcagag actatctggg atcgctggtt taaagaacaa
     1021 ccttaccggt tttatcaacg aatcgattcc tctattacaa ccgatcacat ccatcaatat
     1081 aaagaagata tagaactaat gaaaaaaact gggcataact cctttcgcgt ttctatttct
     1141 tgggcacgaa tgtttccaaa tggcggtacc ggaaaagtaa atcccaaagc catttctttc
     1201 tatcgaaaat tattttccga aatgaataat aacggaatca aagtatttgc caacttatat
     1261 cattttgaca tgcctgctac tcttcaagat aaaggaggat gggaatcacg tgaagtagtt
     1321 gatgcttatg ttcattacgc tgaaacttgt tttaaagaat ttggtgactt agtttgtcat
     1381 tggtttacct tcaatgaacc acttgggcct attttaggta cttatcttga agattttcat
     1441 tatccaaacc tcattgactt taaacgcgga gcgcaagcag cattcttcac tatacttgca
     1501 cacgcaaaag caatacaagt atttaaagaa ctagctcttc ctagtaaaat aggcgttatt
     1561 ctaaatttga gtcctacttt tcctcgtagt caaaatcctg ctgatgtgaa agcagcagaa
     1621 atcgctgatt tattttatac gcggagcttt ttagatccta tggttaaagg gaatttttct
     1681 aaaaagctta tcctgttatt acaagaatat aatcagcttc ctgaagatta tacgaaagaa
     1741 gatcttgtat gtatctacga aaataccgct caaattcttg gactaaacta ttacgagcca
     1801 cgtcgcgtca aatctcgttc ttctatggtg gatacatcta gtccattttt accagagtgg
     1861 tttttcgaac cacatatcat gcctggacga cgaatgaacg aataccgggg atgggaaatt
     1921 tacgaaaaag gaatttatga tttgtgcatg gatatcaaga accattatgg aaacatcgaa
     1981 gcatttatct cggagaacgg aatgggcgtt gcggatgaag aacgtttcat ggatgataca
     2041 gggcaaataa ttgatgatta tcggattgag tatatcaaag atcatcttgc ttacttatgg
     2101 aaggcaataa atgaaggatg taacattaag gggtatcatc tttggacatt tattgattgc
     2161 tggtcatgga tcaacgccta taaaaaccga tatgggctgg tttcactcga ccttactact
     2221 caaaaaagaa caattaaaaa aagtggcgaa ttttacaagc aattaagtga tacaaatgga
     2281 tttagatacg atgtagactt gttagttgaa taatattcta aaattccact tgaagtgaac
     2341 acattagaaa acaagaataa tgagaaatac caataacaaa tactttttct tcatacttca
     2401 tttatacttg tcagtataag agactgtgac aaatgtagtg atcctcagaa gttaggtctg
     2461 gaaatctaac ttgagaagat ttttttaaac tacgtccagt cttttttatt tgtttaaaat
     2521 tgaagttgac aagtcttcat taaaaattta gaatattcac aagtttgacg acaattatga
     2581 aaatttatgt cgtgctaaag gagttttgtt attggatgaa aagttagata taaaaactgc
     2641 gatcaaagac acgttaccta ccgtttttgg ttatatcggt attggacttg catttggtat
     2701 cgttgggaaa gctgccgggt ttcatccgct ggtcgtcacg ttgatgtccc tgctgatcta
     2761 tgctggttct gcccaattta tcacggtcag catgcttgct agccatagtc cactgctctc
     2821 catcgttctc tcgacctttc tagtcaattc ccgaatgatt ctgatgagta tgacgattgc
     2881 tccttatttc aagaaaaatc ggctgcttca aaatctgctg attggcacat tgctgacaga
     2941 tgaaagtttc gcactgggaa tgaataaatt gaactatact ggacaaaagt taaactttcg
     3001 ttggatgaat acagctaatt ggatttctta tttgacgtgg gtgggttctt ctcttgttgg
     3061 cgcactttta gggaatttta ttactgatcc gaaaaagttt ggtttggatt tcgccatcgt
     3121 cgctatgttt atcgggttac tttatcttca agtcatttct gatagaaaca cctctaaacg
     3181 attacagttg atcctgattg gtctcacttt gattctggta tatgtcggac tgatttttat
     3241 cccaagtaac ttagtgattt tagttgtaac actgattggg tgcggattgg gggtgtggat
     3301 caaacatgcc ttcttttgag tttacattgc tgactatttt aggttgtact ttagcaactt
     3361 ggctttctag gatcctacca tttgtcttac tgaaaaaatt cgatttgccg caaccgttgc
     3421 ttgagtattt aagttttgtc ccgatcgtca ttatgtctgc tttatggttc agcagtctat
     3481 ttacccaaaa tatcggacat ctgccgcaga tcaatctcga aaatgctttg gcttcagttc
     3541 ctacactttt agctgctatt ctttcaaaaa gcttattggt gatcgtcttg gtaggaattc
     3601 tttccctttc gttgattcgc ttattataaa taaaaaaaca agagcttatg gcaaattact
     3661 gtcatgcgct cttgttttta tcaaagtcaa ataagaaaaa aatcggcgta ttcttgctta
     3721 tccgttgaac ggaccactaa caacgacttt tcctaatata tgtcctgttt ctacgtcttt
     3781 ggttgcttgt ttcaagttct tcgcattcat cccatctgaa tagaccttgc tcaatgtact
     3841 gcggataatc ccttcatttg ctaaagcagc aatgtgcgct aaagcttctc cttgcgtttc
     3901 gatttcatag ttatagtctg ttttagcgaa catatactcc cagtcgaagc tgatagattg
     3961 gtttttccaa acacctatat ctaacggctc tttgatccct acgatcgttc ccacatgtcc
     4021 aaatggtcgg acaaggtgtt tgatttgatc aaagtagcgg gtaatatcga ataatacagc
     4081 gacatactct acttgatcga ttcctaattc acgaaaactt ttacttaagt cgtgatgata
     4141 atcgattgga tagtctacac ctgttttttt cagccattca aaattcttcg gacttgcagt
     4201 cgcgtagact tctaggccag cccaatgcgc tagttgattc aaaatcgaac cgacgccccc
     4261 agctccgttg atcactaaga tttttttccc gcggttggca ttttcttctg gagtcagacc
     4321 aaatttttca aataacaatt cataagcggt caatgaggtc aacggcaagg ctgcggcttc
     4381 ttcatctgaa agattttttg gtgcaagagc gacaatacgt tcgtcaacca gttggtattc
     4441 ttgattgctt cctgctcttg tcgttgtacc tgcgtaaaaa acgcgatcgc cgacagaaaa
     4501 tttttgcact tcatcaccta cagcaacaac tttcccgact ccatcaaacc ctaagatacg
     4561 taaagcattt cttatcccgt tatcttggcg aagtttcgta tcgacaggat taacagatac
     4621 tgcgttgact tttaccagaa gatcccgtga actgggtaca ggtatctgtt ctttttcatc
     4681 aataaagctt tttggatcat cgatcgacaa cccttcataa aaaccgacta ccttcattaa
     4741 ttccttttct gacatacgaa ttcctcctta gcttcatcaa gttggagcat ccatacagtt
     4801 ttcatttcaa tatatatagt atgctatttg ttccactttt attcatcata attattctct
     4861 tacttaaagt aaactatttg cacttttttg gttaaaaaag aggttgcgac aagacttttg
     4921 tcacaacctc ttttaaacgg tgttcaatca actgaccctc ggtattcgct caaaaaacga
     4981 caaaatatga ggagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt
     5041 tcacgacctc taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga
     5101 tgttcttttt caaaggtgtc taagatctct cgtacttcat cagtagattt cgtattcatc
     5161 aactgaactc gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga
     5221 tgtagtcctg tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc
     5281 agacgaagta gtccgatcag ttctttttct gtatgttccc tcggttcttt ttcaaatgca
     5341 taaggattct tgaaaatccc ccggccaatc atgatgccat ctacgccata tttttcggcc
     5401 aattcttctc ctgtctggcg atctgggata tctccgttga tcgtgatcag tgtttgcgga
     5461 gcgatttggt cacgtaaagc aatgatttta gggatgactt cccaatgtgc atctacttta
     5521 ctcatctctt tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg
     5581 tgactgatcc attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg
     5641 acaggcaaac cgccagcttt agccgcttca atcaattgtg ccgctacttc tgggcgaagg
     5701 atcaagccac tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata
     5761 tcgattcctt ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc
     5821 ttgtctcccc aaatatgggc aaccatcggc tgttcatctt ctgtaaaggt caatcttcct
     5881 cgtacactgt cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag
     5941 aagacatccg gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct
     6001 tccattggtg ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt
     6061 tatttgttac tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc
     6121 attatactag gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga
     6181 gcaaaaaaag actgtgacaa aaatcttatc acaattcctt ttccggaata aatggtgttc
     6241 aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa aataggagga gctatttttg
     6301 tttggctgtt cttattactt gatgcagaac agctttttca caacctctcc actaaacggt
     6361 gttcaatcag ctgaccctcg gtattagctc aacaaacgac aaaatatgag gaactatttt
     6421 cccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tttctaactt
     6481 ctcaatcctc ttcctttatt gatcaaatac caagtaacgg cgtacaaaac aacgatgaat
     6541 agcacaagta cgatcatcga tgttgctact ggcacatcga ttgttccaag gaatccgtaa
     6601 cggaaaccgg agatcatata aacgatcgga ttgactttcg aaacagcttg ccaaaatggc
     6661 ggcaacatag agatggcata gaaaacgcca cctagataag ttaacggctg caagacaaat
     6721 gtaggcacga tcgatacatc gtcataagac tgagcaaaaa tcccgttgag cagtcctgct
     6781 aacgaaaaca agattgccgt catcaataat gtaatgatga ccatgaacca tgaatacaca
     6841 tgaagtggta caaagaacaa ggaaatgatc gttacgagag agccgacgag aatactgcga
     6901 cccactccgc caatcacaaa gccccaaatg atcacatgcg taggaacagg cgctaccaag
     6961 atttcttcga tgtttttctg gaatttttga gagaagaagg acgaagaaac gttggcatac
     7021 gaactagtga tagcagacat cataatcaaa ccaggcacga tgaattccat atatgaaaaa
     7081 ccacccatgt caccgatccg accaccgatc atttttccga aaataacaaa gtacaaggat
     7141 gtcgtgatca caggcggaac cagtgtttgg acccagatac gcaaatagcg gttcgtttcc
     7201 ttgatagcta aactttttaa tgcagtaaaa taaagattaa acatttttct cctccgtatg
     7261 attattttct tctgtgattt tcaaaaacag ttcttctaaa cgattcgact tattcctcat
     7321 agaaagaact ttgattcctt gtcttgtcag ctgatcgaag attccgttca ccccttgatt
     7381 tcgttcaact tcgacagcga ttgtctgttc atcttcaaat gtacaagcgt atccttcaat
     7441 cacaggtttg gtctcatatg gcgctaaatc aaagataaat gtttcaaatt gcagtttgga
     7501 tagaagctct ttcatactag tattctcgat cagttcacct gattggatga tcccgatgtt
     7561 gcgacaaagc atttcagctt cttctaaata atgcgtcgtc aaaatgatcg ttgtaccatt
     7621 ttcgttcaat tctcggagaa actcccacat ctcgcgtctc aattcaatat cgactcctgc
     7681 tgttggttca tccaagatca gcaaacgtgg ttcatgcatc aatgccctag caatcatcag
     7741 tcttcgcttc attcccccgg aaagcatacg tgcacggaca tttctctttt cccaaagatt
     7801 ggactgcttc aaatattttt cgctgcgttt catcgcttct ttgcgagaga caccgtagta
     7861 gcctgcttga ttcacgacga tttgctggac cgtttcaaag ggattgaaat tgaactcttg
     7921 cgggactaat ccaatctgtt gctttgctcg gactagatcc gtatccagat cataaccgaa
     7981 gacattcact tttccagatg ttttattgac taaagaagta atgattccga tcgttgtaga
     8041 cttcccagct ccatttggtc ctaatagcgc atagaaatct ccttctttca cggaaagatc
     8101 gattcctcgc aatgcttcga ccccacttcc ataaaccttt ttcaaattat ttatttcaag
     8161 cgcatttgcc atttatttcc tatctcctta tagacatgat tctttattgt atcatggaat
     8221 acagcagatg aaaaaacaac ttggttccag cgaaaaatca ggttgctggg ctcaggaaaa
     8281 tctcctaatg aaagacatca atcggacgaa gtcagacaac cttttcagaa aaaacaaatc
     8341 gaagataaag gattactatt catatgtgca acaaaaggag cgtgagacaa aagtaaagaa
     8401 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcaaaagcca agatttttga
     8461 tccaataaat actttcgatc acttcgccta tcccaataca tgccactgcc gcttttcatc
     8521 aaataacgcg gcttccgttt ttgcggtttc tactaaagtc tctgggtaat ccaaaacttt
     8581 cagcaaggca atcgcatttc gggtccttga aggtccttct tttaatttga aatcaaaact
     8641 gattcctttt ccttctgtta cttgttcttc aaagtggaca ttctcacaat aattctttaa
     8701 aatctctgtc aattcgatat catgtgtggc gacaaatgct aaagatggat actccgcaag
     8761 ccaccgaacg actgaagcgg aagaagcaat ccgttcgatc gtatttgtcc cttttaaaat
     8821 ctcatctacg aagcagtagc agcgttcttt ggaagccact tggtccaaca aacgtttgat
     8881 cgatttgatt tctgaaacaa aataactgtc gccttcaaat aaatcatctt cgattgccat
     8941 ggaagtcaac acatgtccag gttgcagggt aaactgttct gcaatcgctg tttggatcgt
     9001 ctgtgctaga atacagctga tggctatact tttgacatac gtagattttc ctgatgcatt
     9061 ggaacccgtg actaatgtat tcttctgcca gttgaccgga ttcactaccg cttcttttaa
     9121 caatggatga tagatatctt tcgctgtcac accgccctca gtaaactctg gtcggcaagt
     9181 gatgggcata taagtacggt aattcaatat tgcagcggct acttctaatt ctcccagcaa
     9241 ttcccacagc ttgatggctt cagttgaatg cttttctaat ttagtgagaa cgaaatgata
     9301 ggagataaat ggcagcatga acatgatgtt gacatattca aaaatcaact ctgcttcact
     9361 cccactcttc atacgaaatg aaaaacctaa tttagggatg tctcttaatg gtttagtttg
     9421 ctgtttcagc tcttcttgaa gtggtgtcgg cagttttgct agctgattgg cggctgaaat
     9481 cgttgaaacc aaataactca tgctattcag ctctgtttct agtgtttgtt tcttcactag
     9541 ataatagacc gtattgaata ctaacccacc gattgccaaa tagattccta gtacttgtcc
     9601 taaaagaagc aaacccaaac ccaccagcgg aagcaagcca agaaaaataa ataaacctac
     9661 gcgtccaatc tttttacttt ttccatccgc tagatattgt tttgcaaaat tatgatcttt
     9721 ttttcctaaa cgagcgaatt gatattggat tttttctctc agttgaggat tttcctgata
     9781 aaaagcaatc agtttttcta actgctgatc ttcaccaaaa tcaaagctgc gtaaacgttg
     9841 atacaacgct tctgaaccta cactagaata ggtactgttg attccttcga atacttcgaa
     9901 catatccagg tcataccaag taagatcatc gatttcgcta tcccatgaac ggaacttctt
     9961 ctccgtgagc catgcttcct tcaagctttc ttctttatca aatctcggtt gataaggaat
    10021 tttttcccat ttgctacgaa ctgtttcctt taatttcaaa cgtgaaaaaa tgtctaaagc
    10081 aactaataaa ataacagctg caataataat taaaacgata aattctcttt gacccatatc
    10141 cctctccgtt cctttatttg ttagctttag tatagcactg ttgcgctatt tgaaaagagg
    10201 gtaaaaaaac acctttcaaa ataaaaggtg tttttttctt gtatattaca tatttatctc
    10261 tcatttatca aaattgacaa ataatcatag ggaggaattg ttgttaattc ttgtttattc
    10321 tccagtgcat tgacaactgt cccaagggaa aggatagcct caggtactat tttttcttgt
    10381 tctgttggat tagctaagcg aagaacaaat ccttgttcat gaaaataaga aggataaatc
    10441 gatgatacaa ttaagtcttc aggtaattca atcaatgaaa atttacgagg aacatcaagt
    10501 tcattttgta atggccaaat cttgttatct aacctgtgaa taaatacatt cagtttctgc
    10561 ttttgataag aaatagattg ttctaaccgt ttttctgcct gttttgccac tttgaactca
    10621 ttaaatcttc cttcttctac attaaatcca aaagtgactt tatactcacc tatctcttga
    10681 gctagcggtg tttccatcat gacatgccct tcatttgtcg tatctcctga agctcgccca
    10741 ggtcgccaag caagattagg ttttccaagt tcccctgttg ttgaaaataa ggttatgatt
    10801 aattgatttt ctatacgttc atactctttt aaaccatctg cataaaacgt aaaataatga
    10861 tcattttctg ccacagagac actcttatcg aatatttcta aattaacagg tttctctacc
    10921 atagtttctg accaattcgg aggaatgaca tcaggggtat tttcaataaa tccgttttga
    10981 atttgtgcaa ttgatttttc agatttatct aaaacattaa ttgctaagcg catacgatgc
    11041 gagtaaacag tattatgaat tttgagtgtg ccttcaacga tttgactttc tcccagactg
    11101 aacgatattt cataagatat ttcttccatt tcgggttttt cagataaacg atcttttaaa
    11161 tctttaggaa gttgagcttt tccgtaaaca actaatgttt cttgtaaaga atctttatat
    11221 actttagctg tttctaattt tagagatagt tctgtatctc cttctaatgg tgaataatca
    11281 tacgtatcgc catcatttgc tgaatcgatc aaatgaacga aatcaagata ttgtctatct
    11341 ccaactatca aattcaattg cccatcttta aaagaaagtt tatatttatt gttacttatc
    11401 tcagttttgt ctatctcgat tgctgtttct aatttttcat tatttttctc aaattcaaca
    11461 acagtgtatc ctagtgctgg cacatttaca tttattttta tgtcaagttc ataataagct
    11521 ggttcatcaa aatattcgaa acctgtagca atcatccgat aagcattttt ccgttctgga
    11581 aaataaactt ctttttcaat gatattatag ggactacctt tgaagtgtat tttctttgaa
    11641 tcagctacta catgcacgat tttcgatcca ctataattct cagtcgttgt gttgaaaaca
    11701 aggacttcat tcgattttag ctttaatgca tcagccatcc gttttacaat aaggttttcg
    11761 attccgtcag ctatctcatt agcttctttg attcgatgga aaatatcttc cgcaacatta
    11821 tctgaaacac agccaccaat actatcatga gcctggtttt gcaaaatctt tttccataac
    11881 cgacgaagta ggcctttact gatatggata ccatttcttt ctgcaatcag catgagcggc
    11941 tcaactcgtt ttagaatttt ttgttccaat ttaaaatttt ctattttcat ttttgttctt
    12001 actgagccaa tagaacgatg aacacgagag taaaccggct gacgaagttc acctttatat
    12061 tcaggtaatt cttttttgtt tctgataact tctacataag aaggataatc tgaaataata
    12121 ttgtggaatt tactgtattt attcaacttc tctacgactt gatcaaaatt taaaatcatt
    12181 gacttttgat ctacacccga aggtactaaa atatctttgt tttttccttg catatgaagc
    12241 atttcaactg cttcatccaa cttttctttt gcaaattcag gaatatcttt aattgcgtct
    12301 aaggtcatca ttccagtcat ataaccaaat ggaaaattca tggcataaac aaatttatct
    12361 cccaatcctt tccagataaa ataaggagat ggaaccatcg tttcaaagtt taatcctcgc
    12421 caacacatga aattatcgat ttctacttga tgcaaaagtg taggcaaatt cgcattgaac
    12481 ccaaacgtgt caggcaaata ccctaccatc attggatctc catatttaag gatcgtatca
    12541 ctgattccaa tcattaagtt tcttaaaatt gattcagcat ccactaataa ggcatctgtt
    12601 tgtgcatacc acggtcccac gaatagttgt cctgttttca tcagtcgttg gataacctta
    12661 agcttttgcg gattgatctc caaatactca tcgacgattg aagtttgtcc atctaaacaa
    12721 aagtttactt tagggttcct ttccaattca tctagaactt cagtaaagac ctgatcagac
    12781 aatacgatgg catcttcatt tgtaaaatac cattctctgt cccagtgcgt gtgttgaatt
    12841 atatgtgcat ttaccatatc agattctcct actttccttc ttctactgaa tttacttgtt
    12901 gttctttttt atacttgatg gattttagtg cattgaccat taacgctgta accactcccc
    12961 caataacaat tgatactaaa tataaaggta tattcgtaac aaaaggaatt gccataaagg
    13021 ttgaaagcat tgtttcttga gtaacaccaa aaaatccatt tagaccacat gctactgcag
    13081 tgcccaccac gatgcttggt attactcgta aaggatcttt cacggcaaac gggatcgtcc
    13141 cttcagtaat ccccacaagg cccataatca tagctgtctt tgcattatct cgttccgatt
    13201 catcataaag ttttttgttt aaaattgttg ctaaaaacat tccaaatggc ggaatagcaa
    13261 ttcctaataa catggctgta ctaggtccaa agcttccgct tgaaaaagaa gcaaccccaa
    13321 aagcatatgc gattttattg ataggtcctc ccatatcaaa cgccatcatt cctcctaaaa
    13381 tagccgacat caaagcagca tttgctcctg acatgccttc tagccaatta gtcagtcctg
    13441 ttgtcattgc ttgaataggc gtagctagta cataccataa tatgattcca acaactaatg
    13501 tagtaacaaa aggaatcagc ataattgatt ttagactaat aacttgcatc ggtactttta
    13561 cttttttcaa tgcattagct aaatagccac aagctaatcc agcaaccatt cctccaataa
    13621 atcccatacc aagattttga cacatcatcc cagtgataaa agctggtgct agaccgggac
    13681 ggtcagcaat tgaatatgcg atataggctg aaatcatcgg tacgataaac tgtaatcccg
    13741 tctgaccaat cattcgcagg actccccaga atgtatccgt ctggtcccaa acacctgtgc
    13801 caccaaaaat gactgcaata gaagttagta accctgctgc tacgacaagc gggatcatat
    13861 aggaaacccc actttggaaa tgatttagaa tttctcttcc ttgtttcttg aattccatta
    13921 gtaactcccc cacgatatat tttttatttg ttaattaatg caattgcttc atcaatcgtc
    13981 tcttcaccat cttttactac ctctgaggtc cctactgtta aaatcggttt accgtcaaag
    14041 cgacctcgtt ctgaaacatt tgtatctact gcaaatatga caacatctgc ctcggaaaca
    14101 tccgaaattg aaagtttgtt ttctatcccc atagccccct gtgtttctac tttaatatct
    14161 aaccctttct tcttcgcaaa cttttctaaa ttagcttttg ccatataagt atgggcaatc
    14221 cctgcgatac atgctgttac tgctactatt tttgtcatat gaaattcctc cttttaaaat
    14281 gatagagctt ttaccaattc ttttttattt ctcgaatctt ttattctttt aacaaactcg
    14341 tcatccatca atcttccaga taattctgct aaaatacgta aatgattagt acctttatca
    14401 gtgtctttta ttgctaacaa aaagacgaga tcaactggtt gatcatctaa tgcgccccat
    14461 tcaacagacc gctttaactt ggctacagcc actgttgatt caattactgc taggcttttg
    14521 ccatgaggaa tggcgatatt tttcccaatt cctgtggtca tatggttttc tctgtcaaat
    14581 acgtcttttt gaaattgttt gtggtcagac acaaacccat tagtctctaa caatttcgat
    14641 aatttttcga ctacactttt tttatcagat gcatttaaat ctatcactat attatttata
    14701 ttgatttttt cactgatcat ttataagcct tctttcaata tataaactaa tttttcaaac
    14761 gacgaactcg tttttaaatt tgctaataac tcatgattcg aagcgatttc attgaatgtt
    14821 tcataaattt tattcaattc aggttttact ttttcattaa gtgcaaaaaa gaaaacagaa
    14881 tgaacaagtg tcccatccca atcaattcct tctggattaa cgtagacaca gattactggc
    14941 ttttttataa aatctacttc gatatgcggc atagcaaaat cattcatcgc tgttgaagat
    15001 agtttttctc ttttgattgc tccctctagt atcccttctt tcgcgtaatt ttcttttacc
    15061 aattttttgg taattatatt taaaacgttc acttgattat cctttatttg gtgggtaaaa
    15121 aagcaaaatc gtttatctaa taaatttaga aaagcatctt ctgtttgttc atttaactgt
    15181 agatactgtt ttacttttct gacatctgcc cctaacatca aaggactaac aacaataaca
    15241 ggaaaatttg tattttcaat cggaatcgtg cttataacaa gttcatcagt gacttttcta
    15301 tcttgtagtt cattgatgct tagtacatct tctatctcaa ccatatctgc aaaaccgtta
    15361 tttattcttt gctctaataa ttttgatgtt ccatatccgc tactacaaac taatatcact
    15421 ttcgttttgt caggtttgta acgatccaaa agtgattgaa tatggagtgt tacgtaagcg
    15481 atttcatctt cattaaaatg tatacagaat ctctcttcga tttcctctaa taaatctaca
    15541 cttatcataa atgacctttt aaaagaatat ttgattttgt ccgtataagg gttatagata
    15601 tttactccca atttgagtct tttaactgct gcattgagat gtaatactag attattgatc
    15661 aattcttcat caggataaaa ttcatttaat ttaattctta ctaactcttg tagctcaaca
    15721 cccatatcta aataggaaag cttcgtttga tctaagtcgt gattcatagc atttttttca
    15781 atcgaagtaa tgtgaatgtc aacataagct tgttcatcta ttggcacaat aattgaaaat
    15841 gctttttcaa tcattttgat taaaataagt gtattctttg agatttcttc ttcagaatta
    15901 ttactacgtg ataagaaatt gcttgtttca atcctttcta tcgcaatagc aaggtggata
    15961 gctaaagttt gaaattcata atctgtaaat ttcaattgtg tcttattcat gtatcgatta
    16021 agcaattcgg ctactttttt caatatgtcc ttatttaaga aattattcgc ttcaccaatg
    16081 aaattgatat tcaagtctaa gagatctgtt ttactgttag atgctgtaaa gccaccccaa
    16141 taatagttca caacttctga catcaacctt cttttacttt tttcatcagc tttgatgcgc
    16201 acgccatttc gatctgaaac taaggacatc ccatgcttgt ttagtctttt tctaacatct
    16261 ttcattgtgc tttctaccgt cgacctgctc acgtataatt cgtcagcaag attttgaatc
    16321 tttagagaat cattagtatt caataattta gcaaacacat aagttagacg gtcctccgga
    16381 gagtctgcga tattgatttt atgactggat aataaatttc gaagtgtttc tttttttcca
    16441 ataagcgaaa cgcctttttt aggttgaacc aacatttgta catctggcat tttttggatt
    16501 atttctttca aaattaaaat atctttgcta attgttttcg tagatacata taattgttct
    16561 gataattctt tgtaagttgt gtgctcattt tcaatcaaaa agtatagtat tttcatttgc
    16621 cgttcgctaa attcgatgcc cattatcttc acctcctgaa tacagcttaa gtgtacaat
//
LOCUS       DADJYG010000052        16492 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.052, whole genome shotgun sequence.
ACCESSION   DADJYG010000052 DADJYG010000000
VERSION     DADJYG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.052"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            55..1227
                     /locus_tag="JZ382_001341"
     CDS             55..1227
                     /locus_tag="JZ382_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY2427537.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1214..1918
                     /locus_tag="JZ382_001342"
     CDS             1214..1918
                     /locus_tag="JZ382_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAY2427538.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1923..3083
                     /locus_tag="JZ382_001343"
     CDS             1923..3083
                     /locus_tag="JZ382_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY2427539.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3159..3437
                     /locus_tag="JZ382_001344"
     CDS             3159..3437
                     /locus_tag="JZ382_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY2427540.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3418..3789
                     /locus_tag="JZ382_001345"
     CDS             3418..3789
                     /locus_tag="JZ382_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAY2427541.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3805..4209
                     /locus_tag="JZ382_001346"
     CDS             3805..4209
                     /locus_tag="JZ382_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427542.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4206..4616
                     /locus_tag="JZ382_001347"
     CDS             4206..4616
                     /locus_tag="JZ382_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427543.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4677..5264
                     /locus_tag="JZ382_001348"
     CDS             4677..5264
                     /locus_tag="JZ382_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY2427544.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5337..5804
                     /locus_tag="JZ382_001349"
     CDS             5337..5804
                     /locus_tag="JZ382_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAY2427545.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5850..6158
                     /locus_tag="JZ382_001350"
     CDS             5850..6158
                     /locus_tag="JZ382_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427546.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6353..13087
                     /locus_tag="JZ382_001351"
     CDS             6353..13087
                     /locus_tag="JZ382_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY2427547.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13091..13963
                     /locus_tag="JZ382_001352"
     CDS             13091..13963
                     /locus_tag="JZ382_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427548.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13960..14587
                     /locus_tag="JZ382_001353"
                     /pseudo
     CDS             13960..14587
                     /locus_tag="JZ382_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14548..15009
                     /locus_tag="JZ382_001354"
     CDS             14548..15009
                     /locus_tag="JZ382_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427549.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15013..15837
                     /locus_tag="JZ382_001355"
     CDS             15013..15837
                     /locus_tag="JZ382_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427550.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15855..16400
                     /locus_tag="JZ382_001356"
                     /pseudo
     CDS             15855..16400
                     /locus_tag="JZ382_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         5891 a         2540 c         3527 g         4534 t
ORIGIN      
        1 ctaagctcta gaaatagggc ttttttattt ttctttgaaa ggtggtgaaa acatgtgagc
       61 ttatttgata tctttaagca gtcaataaga aatgaagagc cttctgagtg gataccggat
      121 tttgtatatg gagaggaagt atctgctcgg gcatacctca aaattatggc aaaaaatact
      181 gttttagatt ttgttgcaag aactatgtca acgttagaag ttaagtttaa aaataaagat
      241 gggacatcag actgggatta tattctaaat gttcgaccta attctgatat gtcagcagct
      301 acattttggc aaaaattttt ttatcggctc ttagatgaca acgaagtgtt agtaatattt
      361 acagatgata atcaattgct gattgctgat gattttagtc gaacggagta tgccgtgtat
      421 gatgatgttt ttacaaatgt gatggtaaaa aattttgtct ttgaacgttc atggaacatg
      481 tctgacgtca tttatatgga atataataac gaagaattag aaaggtttac acgaggttta
      541 tttgaagatt acgccgcgtt gttcggacgg attctagaag ttgcaatgcg taacaaccaa
      601 attcgtgggt ctgtatccat tgattcaacg ggaacggcga atgatgaaaa aggaaaagat
      661 ggtaagacta gatctcaaag attacaagaa tatattgata ggatttggaa ctcttttaaa
      721 actaactcag ttgcaattgt tcccaaagtg aaaggctttg agtatgagga atacaccaat
      781 aaacaaggcg tatcgaatca atcgctagat gaactgaata agatgaagtc gtctttaatt
      841 gatgatgttg ccaacgccat aggagtacct acggcgctta tttatggtga aaaagctgaa
      901 ctggattcaa atattaaagc ttttcgtaaa ttgtgtattg ctccactagt taaaaaactt
      961 caagacgaat tggtggcaaa aataattagt cgtcaagagt acaaaaacgg ggaacgtata
     1021 gaagttaaaa aagttctcca aaaagatcta ttagagtatg ctactcaaat tgataaaata
     1081 acatctggtg gtgcgtttta tattgacgaa gttcgtgatg aagttgatta tgatgagttg
     1141 ccaaataatg aaggaaaaat tcgtattcgt acgaaaaact atgaagaaac tgtgaaggga
     1201 ggtgagaacg acaatgccga aagctaaaaa agtaccattt cagtttacta acgaaattca
     1261 gaatggtaag cacattctca ccttgagtgg aaatgtacag aaaaaatatt ggcgtgatga
     1321 tgatgtcata aacgctaaag atattcgtga ctctctagat actgtaaccg atgatattgt
     1381 aatcaagttg aacagcccag gcggtgatgt gtttgaaggg attgagattt acaattattt
     1441 aaaagatcac ccatcaaata ttactgtaga agttacaggg cttgccgcaa gcgcagcaac
     1501 gtttattgtc gctggtgcca atgatgtgat tatgaatgtt gggacttctt tgatgattca
     1561 tgaagcttct acatttgctt gggggaataa acaggacatc caaaaaacgt taaacgcatt
     1621 ggaaacgatc gatgattcta tcttagctat ttattcagat aagactggcc aatctgttga
     1681 taagttgcgt gaatggatga atgaagaaaa atggtttaca gcagatgaag ctgtcaaata
     1741 tggatttgcc aattctgtga agcgtgaaga accacaaaat gaacctcaaa atattgcagc
     1801 gatgattcaa gatgctgttg ccgttgcaat agcgaattta aaggaacctg ttactaataa
     1861 aatcgaacaa gaatcaaaac aaaaatcatt aatcgcacga ttgcgaaaag gagaataaag
     1921 atatgttaaa aattacagat aaaactgcag atgcaaaaaa ggcatttaat gctatttctt
     1981 cgaaagaaga tgcaacaccc gaacaaatta atgctgctct agaagaatat gtaaccgcca
     2041 ttgcagaaga tgccggcaaa caggtgcgtg ctgaatatga agaattgaaa aatgtgacgg
     2101 acaaccgtgt attagaggct cgtggaattc ctacactaac agcagaagaa acaaagtttt
     2161 ataatgaagc cgtaaaatct ggaggtttcg attctgacgt agtttggcca gaaacgattt
     2221 tggaacgtgt ttttgaaaat cttcaaaacg atcatcctat tttaggcctc attaacttta
     2281 caccaactgt aggtcgtgtc aaagtaatta gagcacgtcg taaaggtgtt gctgtttttg
     2341 gaccattgca taaagacctt gaaggccaac tagatgctga atttggtgca actgaatttg
     2401 ttcaacttgc gttgaccgca tttttcttaa tttctaatga taccttagat ttaggaccac
     2461 gatggattga ccgttttatc aatctttctt taagtgaagc agtacgtgat gtttgggcta
     2521 aaaaaattat tacaggttct ggtaaagatg agccaattgg attgcttaaa gatctagatg
     2581 gtgcggtcac aagtggtgta tatccagata aagctagcgc agggacatta acttttgcga
     2641 aagagaagat tgttcttgaa cttgcagggg tgatgaaaaa attatctaaa tacacataca
     2701 aagtagatgg tgacgatgaa ggtgaagtga aataccgttc tgttgctgga cgagtgtatc
     2761 taattgttaa tcctgttgat tactgggata tcgtagctag agttacattt gcaaatttaa
     2821 acaatgtgta tgggactacg atgccattta tcgctgttga tcatatcatc gaatctgtag
     2881 acgtaccaga aagtaaatgt attgcatacg ttgataaaga gtatgaagct actcaatcaa
     2941 gagctgaaaa agtctatgta tataaagaaa cttttgcaat gaaacgcgca actttgtatg
     3001 ctgtcgacat gcttggtaat ggctatccaa caaataatga tgcagcacaa gtatatgact
     3061 tggactttac agcgggggag taatgtcccc tgcaattggt gagataacac cgacgactga
     3121 tggggcagtc attaatcttt cgtaaatggg gttagagtat gaacactgaa gaatttataa
     3181 aagaatataa ggcccgattt cgtattttcc atacttccga agatgaggat atcgggaaac
     3241 agttagagag cgggtttgcc gatataaaat ctctaatcgg cagttttgat ccttccactt
     3301 atgagaaagg aaaagaatta gtttatgaac gtactcggta tctacggaac gaatcactag
     3361 agtactttta cgacaatttt caaacaatga ttatggatgc atcaattgat ctggcaggtg
     3421 ctggatatgc caattaaatc aaattataag aagcctaaaa ttgtagctgg tgatctaaat
     3481 acgccagtta ctttttttga gtttcaacct aataagggtc cagaacctgg tgatgaagaa
     3541 aaaaaggaac tgtatttttg tacagcacta gtttacaacc catcaatgaa agatcgtgac
     3601 attcttggca caaaagaagg cgttacgata aaaatacggg atccccatca atcttatatt
     3661 cctactaaca aacataaagt aaggatatta gattatcgat atggagataa agtttgggaa
     3721 ataatcgatg tctcaccgga tttggaagaa aattcaattg taaagatagt attagaatta
     3781 attacttaaa tttggtgata aatgatgagt aacgtaaagt ttgaaggcgt tcaagaaaca
     3841 atccaagctt tagaaaaaaa gtatggagaa aagaaaacta agacattaac aaaaaaagca
     3901 atcaatgttg gcgctgaaaa agtagaaaaa cagttgcaaa cggacatgac cgtttttaaa
     3961 gacaaaggtt atacgatcgc tgaggttgtt cgcaagaacg ctacgtatag agattacaag
     4021 gcagaagctg aaatcggttg gaatggtcca catcaacgat atagaatcat tcatttgaat
     4081 gaatggggat atacacgaaa gggcaaacaa attagaccga gaggatttgg tgtaataaca
     4141 aaatctctta agaattccga gctgttgtac tttgatgctg taggagagga ggttaaaaag
     4201 agtttatgaa agatatgctt aatatcattt acgaggctct ttgtttgaat gagtatattc
     4261 attcaatgac ctacaacgag aataccgagc aatatcgtat taaattttat gagcaacctg
     4321 aaaccgccga taaaacaggt ccaatgatca caattcgtcc tgtagataca cctaacgaag
     4381 cgtaccacgg aagtaacaaa gagctttctg tggagcatat gatacagatt gacgttgaat
     4441 cgaaatatag agcaacatgc aagcagattc aatatgagat aaaaaaagag atgaaaaaac
     4501 taggctttgg ccaaatttcc ggtcaagggt tagatgaata ctttactgaa acaaaccgct
     4561 ttgtagacgc gcgtcgttac gaaggaaata caaaaattta cgataccgaa tattagaaat
     4621 taagacacga aaataacgtg tcttttttta ttacccaaaa ttaggaggaa aattttatga
     4681 ctttagtagg attcaaaaaa atgacaatcg gggtctttga cagcacaggg aagattccag
     4741 cagcaaattt atatgtaatt gaaggtgaac aaaacaaagg ggcgactgta tctgctgaaa
     4801 tcagtgggct atcaaaagaa ccatcaaaag tttacggatc gaacatcgct tactatgtct
     4861 ctcaaaaagg aacaggcgat gtttcagcta cttttggatt gttagattta ccaacagaag
     4921 taaatgacaa aattcttggt tacaaaaccg atacaaacaa aatcagcttc ttgggagaag
     4981 atactgagcc accatattgt gcaattttga tggaatcgga ggatttgaac ggcgatacag
     5041 caatgcttgc gatgttcaaa ggaaaattca gtcgtgaatc tattaatctt aacactacga
     5101 caaatgaagc tttcgaacca gaagcagaag aatatgtatt ttcagcgatt gccaatgatg
     5161 ttgaaggtga tgctaaaggc caaacagtcg caaaatatgt tggagacgaa gaagcttcta
     5221 ttactggttt gaaagagatg gtatttcctg cagcggggga gtaactagcc ccgttgttgg
     5281 agcaatcact cccactatta atggggcaac agttgaatta agttaggagg ataactatgc
     5341 cagatacatt taaaatctat aaaaaaaatg gtactgaatt tacaaaagtc gcagagggtg
     5401 aaagtccact ttctattact ggaattgcag caaatactca agttgctaaa ggagattatc
     5461 aagctacgcg tgtggtcgga ggtcaagaat cagtaaaagt agatattcct gcattcacca
     5521 ctcttccaat ttcagttact ggtgtgacgt tggataaaac gacagctgaa gttgaacaag
     5581 gcggaacctt aaaattaact cctactgtaa caccagcaaa tgcaacagac aaaacaggaa
     5641 gctggagcag ttcgaataca gctgtagcta cggttactgg aggcaacgtt acagtaaaaa
     5701 cagacgctga agttggcgga actacagaag tttcatttac tactacggat ggcggtaaag
     5761 tagcaaaatg tactattaca gttattgaaa aagcagcggg ctagtcttcg gactagcttt
     5821 tttatttgga aaataatgga ggaatctaaa tggcaaaagt aagaattgaa ttgaaagaag
     5881 aatcaggaaa gaaagtctat gaaaacatag acacaacagg gaaagattat cgaaaagctt
     5941 tagaaacaat caaaaagcta aatgaagacg gagtaatgat ttggaatcgt ttagacattt
     6001 atttggattt tgctgtaggt atcttcaggg atagcaaatt gacagctgaa caaattcttg
     6061 atggcttacc atctgagaag gtaatgagca cattggacga catcctagga gaagtaatgg
     6121 ggattgaaag taatccagac ccagacgcaa aaaagtaaca cccgaagaag cagaagaaat
     6181 gtacttaaac ctatgcagag agttagtgaa agcgggatgg tcactatctg acatagaaga
     6241 taattccttt gaaatgatga tgaaaattgc gtgcacttcg cctaagaaag agaaacaaaa
     6301 agaagttgat cttaaagact tcttgaaatc catgtagaga ggaggtaaac atatggcaaa
     6361 tggaaaacct ttaggcaata tgattatcaa actagactta gacagttcag ctttttcaaa
     6421 aggtctagcc ggagctaaaa acgctgttaa ccatcaaatg aaagcaatga aatcacagat
     6481 gcaagtaatg aactcttcag gaaattctct cggtgcttta caagcaaaat acaatggttt
     6541 aggcggagtt ctacaagcaa atgaaaagca agtagaattg ctcactaagg cttataagga
     6601 tagttttgac tctaacggaa acgctactgc ttcgactgct aaatacgcta atcaattgaa
     6661 tcaagcaaca gctagatcag caagttacga atctcagatg aaaacaactg ttggacagat
     6721 tgctcggatg aaagtggaaa cggaagggtt tacggggaaa ctaaaggcta attctgatgt
     6781 attgattaat tctgggaaaa aaattgaagc ctttggtaaa ggagtttctg ggataggatc
     6841 tgccctaact gttggagtaa cagctccaat agtggcaggc gcaactgctg ttactagtgc
     6901 agctatttca tgggaaagtg cttttgcagg tgtaaaaaag acaaatgatg aagttgtaga
     6961 ctcgaatgga aacgtagttt attcctatag cgacttagag aaaggtctgc gtaatcttgc
     7021 cacccaatta cctggtagcc atcaagaaat agcaaatgta gccgaagcag ccgggcaatt
     7081 aggtataaag actgaaaacg tagttagctt cactaaaacg atgatagaca tgggtgaatc
     7141 gactaatatg tcggctgaaa ctgcggcaac ttctttagca cgatttgcta atattaccca
     7201 aatgtctcaa aaagactttg ataaattagg atcagtcatt gttgatctag gtaacaattt
     7261 tgctacaacc gaatcagaaa tcactgaaat gggattgcgg ttagcgggag ctggtaaaca
     7321 gattggaatg agccaaggcg aaattctagg atttgctact gctctatctt ctgttggtgt
     7381 cgaagctgaa gcgggtgggt ctgccttttc gaaggtcatg atccagatgc aactagctgt
     7441 agaaaaagga tttggcgctt ttgatcagct gaaacagatg gcagaagaac aaggtgttcc
     7501 gtgggttaat ttagtaaatg ctgtgagaga cggtggaaaa tcgttaaaag cagtatcaga
     7561 gcaaatggga ttcacttcat ctgatttaaa gaaaatgtat aaagaagcgg acaattcgaa
     7621 aagttcgctt gaaaattttg cgaatgtggc aggtgttaca agtgaacaat tccagaagtt
     7681 gttcaagagt gatccttcag aagcaatcat caagttcatt caagggctga aagattcaga
     7741 aaaacatggt acttcagcaa tcaaagttct ggatgacatg gatataaaag aagttcgact
     7801 acgcgacagt ttattgagag ctgcaaatgc tagtgacata ttcaaaggag caattgaaaa
     7861 aggaaacggc gcgtggaaac aaaatacagc tttaacaaat gaagctaata aaagatacga
     7921 aacaactgaa tcaaagctaa agatattgaa aaacgaagtg attgatacag caatcgatct
     7981 tggaggtcca tttgtagacg cgttgcgtga tggtctaaat gtatcgaaac cattgataaa
     8041 aaacttaggg aatatggcga aagctttctc agctatgagt gatgatcaac aaagaaacat
     8101 tatcaaatgg gttggattag cagcagcagc tggtccagcg ctgaagcttt taggaactgg
     8161 tacttcagta ataggtaaag ttacttcaac tactggaaga ttgacgaaaa gccttgttga
     8221 tttagcagcg aaagcagcag aaaagaaggc tatggatgcc tttgcatcgt cggtaacaac
     8281 agctggtgct gcagctgcaa agacagcagg agctggaggt attggcgggt tagtccctat
     8341 gatgggaaaa acagctagtg ctgcaagtac tgctgcgggc gccagcggaa taggcgcaat
     8401 gacaacgtca ctaggtttgt taggtcctgc tctacttggt attgttggcg ttggtggcgc
     8461 actggcagtt ggctatggag catggaaatt atttggtgaa gaagcttgga attcctcgca
     8521 aagagtaaag caatggggaa cagatgtcgg tgaggagata gatagtactc ttgacggagt
     8581 acagaagaat attgaaggtg caaatggtca attcggtctt ctcaaagagg gttttactgt
     8641 tgatgatgca aataatatgg cggctaattt cgcaaccgct ggaacatctt tggaaacatc
     8701 tttaacgggt agaataaagg cattagacga tgcactaaaa gggctacctc aatcagttca
     8761 ggatgccatg aaagaagtag ctgaatcaga agaagaaacc ttagggcaat ctctttctaa
     8821 aataaaagac aacaatgaag aaataaaaaa aatcagagaa agagccgcaa atcagaacag
     8881 agacttgact gtttcagaac tacaacagat acaggatcta atgaaaggaa cttctcaagc
     8941 gtatgtggat acgctcgatg tttctgctaa agacagaaaa aagatactat cagccatgaa
     9001 tggtgatgtt gcacaagcta cagaagaaga agcgaagacg tggttacaat ctttagcgaa
     9061 acaaaaagct gcaactaccg aaaattatag acaaatgctt gaagaaaaaa aggcggcttt
     9121 aaaggaaaag ggagtaagca aagaattcat tgatgcatta gaagatgaaa tgaatgatta
     9181 catcaaaact acaaatcaag gattcgatag ccaaatagct gctataacaa ataaatatcc
     9241 acaattgatt aacgaagtga gtttatcaaa tggtcaatta tttagcaaaa cggcagctgc
     9301 tatggacaat actggtcagt atacaaaagt ttttctcgaa agtaatgaaa agattttagc
     9361 ccaagcgaag aaaacatcgg atgaaatagc agataatgcc gaaaaaaatg cagaacaact
     9421 tggtatggta gctgatgagg ctacgcatgc tggatcaact tggaatggct tagttttaga
     9481 cgaaaaaaca ggaaaagtaa aaactaatgt acaagaagta gtgaatgaag caacgaagga
     9541 tttcactaaa tggaatgaat tgaaacctgt tgttcatgat gcgaaactaa caacgaatgc
     9601 aaaagatgtc atttccatag cggcagtaca aaatggctat tgggatgata tggaatggaa
     9661 agaaaaagaa gcattattag aagatgagtg ttctaaaaat gtatacaagg ctttagaaaa
     9721 tgctggtaaa tggaatgagt tagatattcc ttctaaagaa gcaatcctaa cttcaaatac
     9781 tcctgaagtt atgggtgaaa ctctcatgaa tttgggatta tgggatactt acaaacttca
     9841 agtgaaagat ttagatttga atgcgtataa agtatacgat gctatcgatg gatcagaaga
     9901 aaaattaaat agttgggctc aattagataa ccccacaaaa gaattgttat tagataactc
     9961 agatttttct acaaaacttt ttaattccga agaaatgttt aaacgttgga atcagttatc
    10021 atctaatgca aagtttcttt cggctaataa tactaaattt acagaggttc ttttatcatc
    10081 tgacagaatg tggaatcaat gggtaaacct tcctgataat gagaaaagat tacttgcaga
    10141 taacgaagat ttagctacca ctgttttatc ttcagaagaa atatacggaa gatggactca
    10201 attaccagat aatcaaaaat atatgttagc tgacaatact gacttagcaa acaaaatatt
    10261 cgcttcagaa gaatcatgga atgcatggaa agcaattcca gacacagtta aaaacttgct
    10321 tggtaataat attgatttac tggctaaagt gaaagacggg actgtaagtg tagaagaata
    10381 taacaaagtt atgccacttc ttaaaactct atatggagat aacgttaatt taaaagaggc
    10441 aatcagtggg gctaaaagct ctatagagaa ttacaacaaa aatatttttc ctagtgataa
    10501 aactgcagta ggacacgatg aggcttcaca agctgctaaa gatgcatttg aagcgttcaa
    10561 catcttccaa acaaaaatac cagataaaat tactaaaact gtatcagctg actttatagg
    10621 tcctatgccg aaaaatgcga aaggaacaaa cttccatcct ggcggagcag ctatggtaaa
    10681 cgatcaaaaa gggcctactt atgaagagct tataaccttg ccaagcggag aaacttttat
    10741 acctaaagga cgtaatgtcg ttttagactt accaagaggt tcgaaagtac tgaacgccac
    10801 taaaactaaa cgtctagtgc ctaaatatgc tgatggtata ggaaacataa cgacggtttc
    10861 atctacgcta aacttagatg ctttaatttt agctattaac gagctaacga cggtattgag
    10921 aactcaactg atggcaggaa cgactgatgc taaagatact aaactatcag atgaaacaaa
    10981 agttgccgat ccgattattc cggacagtct ttctgaaaaa tcagatcagt atctaggaat
    11041 tggagctcaa tggttaacta atctaatgaa tggttggaac tcagcggttc ctcaatatat
    11101 gaatagtgaa atggttttta ttacaaacta tctcaatgcg ctaaaacttc aaaacaatcc
    11161 taattatacg caaggagcaa catggaataa gaacttactg aatggatgga atagtttaac
    11221 tgggaccttt attgctacaa tcaattcatt ttgtaatcaa gcaatggtca cgcttagaaa
    11281 ctacaacact cctatgtaca acaatggccg aacgtggcag caaaataatc ttaatggttg
    11341 gaattcattg tatggatcat tcatagcccg tgtaaatcag cttggtaacg attcgattaa
    11401 caaccttcgt tctaaaagtg gtggttttta caatgcaggg acattcttgt tgcaatcttt
    11461 aataaacgga atgaattcgt taggtaattc tctttctact acaatgaaca atgtcgctaa
    11521 tacaatggtt ggtggtatag gcaaaggagt taatggcgtc atatcgggag ttaactatgt
    11581 gcttaaggaa gtcgaatcaa gtaagaatat cggaaactgg ccaattccac aatacgctaa
    11641 gggaactgat ggacatcctg gtggacttgc tatgattaat gatcaaaaag gcctagttca
    11701 cgaagaatat gttcagatgc cagatggacg tagttttatt gccaaaggta gagatcttct
    11761 tgttaatttg cctaaaggtg ctcaggtctt aaatgcttct ttaacaaaga aattaaagga
    11821 acgtttaaat attccacact acgaaaaagg tattggcaat ttagatattg ttgatttgtt
    11881 agatgatgaa aaaagaatgt tagaattctt aactagcaaa gttgattttt caggcattaa
    11941 tgaaccatgg cttgatatga ctaaatcagg aacgtcatta atgtccaaag ctgcaaatac
    12001 aatgcttcaa tcaaaattaa gtgaattctt cactcatggc aattttgatg gtgcagtaaa
    12061 tgccaatggt gtttatcaat atttagttga tgtggcacag aaagtaatgg gtaagttccc
    12121 cggacttaca gtaacttctg gatatcgagc aggagatgcc tactatcacg gaaaacggca
    12181 agctatcgac ttggcttatc caggtatttc tggagatccg agatatacag cagcagcaaa
    12241 ttacgctttt gaaaaattcc cttcaaaaat tgcgtatgtc attacgaatg gacgtgtacg
    12301 tgaccgcatg ggattatctg gaacaaggtc aagcggacaa tggacgaatt ggccagatgg
    12361 cgatcacttc gatcatattc atttaaacgg ttcgatgggg tctggcgata tattcaatgg
    12421 aggagttgga ggaagtggtg ttgcacgctg gcgttcttat gtgtctaaag cattgaaaat
    12481 gaatggatta ccagctacag cagcgtatat aaatgcctgg atgtcccaaa tccaaacgga
    12541 atctggtggt aatgagaaag caatcggcgg caatgatggg ctcgccgaag gcaatgcaac
    12601 aggattactt caaacgaaac ccggaacttt tgcagctaac gcttttcctg gtcacggaaa
    12661 tattatgaac ggatttgata acatgttagc agcaatcaat tatgctaaga aacgttacgg
    12721 agttgcaggt atgttgcaag ttatcgggaa aggacatgga tatgccaatg gcggcttgat
    12781 taccaaagac ggactatatc gagcaggaga aggaaataaa ccagaaatgg ttattccatt
    12841 gacaagaaaa acaagagcta tcgaacttat gggtcaagca ttagccttcc tttctggaag
    12901 caataaaaaa gaaacgaaaa gtgagattaa ttctgtagat aatagacata tcctacaagc
    12961 gatagaaaaa cagtctcgta tgacaaatga tctgctatca ttattactct cgttttttaa
    13021 aagtaacaat cgatcagata aagaactagc tttagatatt cagaaaattt taatcaggag
    13081 gacatagtat ttgaggacag taatattaag aaataaattg aatgaagaaa ttgatttgtc
    13141 cacagaagat tattttgcta ctgaaatgtc gaatctaggt tttgaagtta caaaagagca
    13201 tattggacaa tgggggagtt tcagagaaat aggagagagt attgaattgt ctgaatttca
    13261 atcatcagtt atgatttctg ttcacggatt tagagaacaa caattatatg gttcattagt
    13321 tgaatttctg gctaatggac cttttgaact agaattttct tttgattcag aaacaatgat
    13381 caggaaatgt agtttgaaat cattatctaa aacagaaatt gattcccaaa gctcattgct
    13441 aacagatact ttgaatctat attttacttc aagctggtat tctattaagc gtgaaaaatt
    13501 aatacagaat tcaacttctc tcaatacacg tggaaaagtt tactcatttg ttcgaccata
    13561 tgtctacacc aaaaatgtat gggaaaagaa aggtgtattt aagttcagta ataattctgt
    13621 atatttgaca aatagtacag agcgaatgtc tccgttaaaa ataaggatta ttggagagtg
    13681 ctcaaatcca tattgggaag ttatccaaaa ctctaaagta gtagcttcag atggctattt
    13741 tattaatatg aaagacacac aaatattaga agtgtctagt ttgctagaag accaaacggc
    13801 aattttaaaa gatgtaggtg gagttgaatc ttctgtttat caacaacaag attttactaa
    13861 atcaaacttc gtccaagcgc ctctaggaga attcagcgtt gtttttcatg ttggacatgc
    13921 aaatgtagag attgaacttt atgaggagcg tgatctattt tgattttagc cattaccctc
    13981 tttcaccgag atatgaattt atataacgaa cgtttaattt cgactgagtt cgaatttgga
    14041 acggatgaaa ttggagaaga agtaagtaat tttattacag ataaatatgt ccatgtaaaa
    14101 tcaggagatt ttctactagc aaaatttatt cctagcggga agattgctta ttttggagtg
    14161 attagctcgc aagaagacaa aaaaataaat tgcaaaggat tactcagttt ggccgatagc
    14221 gagataccga ctgtaaaaat atcaggtgct aattatgaag agcatattcg acgactgatt
    14281 gaatactact tattgaacga tccaacaaaa caactaaaaa atattttgga ggtaaaaaca
    14341 gaaagcgtta cttctcattc ttatcaagct gttgacacaa ataagagacg attgaatgca
    14401 tatataataa atgggttcaa aaaatataat gttaaatggt atgtcaaaga gattaaaggt
    14461 aaaaaaatat atactggtat tcgagcagtt gatgattcaa tttatattaa agataattca
    14521 tctgagttta atgactggga tgtttttgtg caatctcctg gaccagggaa cgagaacaaa
    14581 ttattgatcg tagataaagc aatgaaagat atagaaaatc ccttggtatt gtctacgtgg
    14641 tatttagatg acgaaaataa tcttacgcaa gattcaacga ataagaacat tgcattgcct
    14701 accgttaatt tagtaaatat atatgatcaa actcaagaag atagaccttc ttatgaagag
    14761 gtagctaaat cggagcttaa aggcaacgcc tattcacatg aaattaaagt taatgttgtg
    14821 ctaaatgcta gaaatatcaa tgtagaaaat atcgaaacag gaatgttagc gactattgtt
    14881 tacaaagagc agacttacaa atctgttcta actgcctggc gaatatccag cagtaaaaag
    14941 aatatagaat tgacatttgg taatgtccga agtcgtttta tggattattt tgaagataat
    15001 gggggataaa aaatggttag caatgtggat ggatatcaat ttgaaaacgt gaaagtaagc
    15061 gcagaaaatg atgctagact ttatcacgtt ttatataatc ggaaaaatca ggttattgat
    15121 ggttacgatc agtctatgaa tttatcttca agcggattaa cagtaaaagt tgctgcagga
    15181 gcagcgatta ttcaaggtcg tatggtcgtt gttcgacaag aagaaagtat aacagttcca
    15241 gcaaactcaa gtggttatat agcattaaca gtggatttga cacaagaagt tatacctgga
    15301 tctattcttc cagaatcgga agaatatgaa tggactaata atcaagtcaa gctagaattt
    15361 ataacaaaag ttataaaagg taatttaaac aatggtgata aggtctataa tttaccacta
    15421 tgctcagtta attctactgg atcaactgtt tcaatctcaa agatatcgga tagttacgag
    15481 ctgactctct ctaaaggaga aattttgtgg agggggactg cgttaatgca tgatactcaa
    15541 actgtccaac cttcaaaaaa aatttggcag acagtttcag gttttttgtt agtatggctc
    15601 ccatacgaaa acgggcaagc aattgaggat agatatgtaa ctacgccttt ttataaggag
    15661 cgtataactt ttactaatgt tttaggagaa attgtttcag gatttgatga ctatcacaaa
    15721 aagtggttta gtaaacgaat aaactataat tcgaatacta atatatttac aggtgcagca
    15781 agcaatgcaa gtggagataa cgcaaatatg gtgcttaggt atatagtttc tttttagtta
    15841 ggaggtatgg aagagtggca aatttagaaa ttaaattatc tgcaaataag agacagcctt
    15901 atctacgtca ccgtgttgtt ggtagagttg gtgatggggg gctcacaaca atcaatgtac
    15961 aacttcttga ggaagatgaa attacacctt ttgtaattaa tctaaacggt actttgaaat
    16021 ttgtgggcga agtttcaaac ggtaactata ccgagggaga accagaaata atcgattcga
    16081 ctaatgggtt aattagttac acgttcacta agtcaaattt cagcacgagt catcaattca
    16141 aacaagcata ttttgaatat gtagatccta acggcaaaaa agtaactttt cagaacttca
    16201 tcatagacgt gttagaacga gtagatatta attcggagca agcgaaatac tatatttctt
    16261 cattggaaaa attacagagt gaaatgcaaa ccactttcaa tcagttcatt agtgataaac
    16321 aggtccaata cgatcaaatc tactcgaaat ataacgaatt agtaagattg ataaatgaat
    16381 cagataagca agtaaatgat cgtattgata aggcaaataa tcgtattgat caaaccaatc
    16441 agcaaatcgg cgatctcggc aagctgaaaa agatgtacag taacagcatt ga
//
LOCUS       DADJYG010000053        16333 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.053, whole genome shotgun sequence.
ACCESSION   DADJYG010000053 DADJYG010000000
VERSION     DADJYG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.053"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="JZ382_001357"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="JZ382_001357"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAY2427551.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="JZ382_001358"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="JZ382_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAY2427552.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="JZ382_001359"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="JZ382_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAY2427553.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="JZ382_001360"
     CDS             2856..3731
                     /locus_tag="JZ382_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAY2427554.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="JZ382_001361"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="JZ382_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY2427555.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="JZ382_001362"
     CDS             4628..5062
                     /locus_tag="JZ382_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY2427556.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="JZ382_001363"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="JZ382_001363"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY2427557.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="JZ382_001364"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="JZ382_001364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY2427558.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="JZ382_001365"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="JZ382_001365"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY2427559.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="JZ382_001366"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="JZ382_001366"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAY2427560.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="JZ382_001367"
     CDS             13333..14259
                     /locus_tag="JZ382_001367"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAY2427561.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="JZ382_001368"
     CDS             14441..15334
                     /locus_tag="JZ382_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAY2427562.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="JZ382_001369"
     CDS             15468..15593
                     /locus_tag="JZ382_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427563.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..>16333)
                     /locus_tag="JZ382_001370"
     CDS             complement(15727..>16333)
                     /locus_tag="JZ382_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427564.1"
                     /translation="FLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLDHSDITE
                     AEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFN
                     HNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALM
                     PLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5803 a         2784 c         3487 g         4259 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaa
//
LOCUS       DADJYG010000054        16327 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.054, whole genome shotgun sequence.
ACCESSION   DADJYG010000054 DADJYG010000000
VERSION     DADJYG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.054"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            57..299
                     /locus_tag="JZ382_001371"
     CDS             57..299
                     /locus_tag="JZ382_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002164449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY2427565.1"
                     /translation="MKRDHYECQECRRLGKYHRVENVHHIKEVKDRPDLALDLDNLIC
                     LCVEHHNEVHGRYLTALDKQEKKIESFANFDASERW"
     gene            304..585
                     /locus_tag="JZ382_001372"
     CDS             304..585
                     /locus_tag="JZ382_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427566.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            688..1002
                     /locus_tag="JZ382_001373"
     CDS             688..1002
                     /locus_tag="JZ382_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAY2427567.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            980..2674
                     /locus_tag="JZ382_001374"
     CDS             980..2674
                     /locus_tag="JZ382_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY2427568.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            2694..3872
                     /locus_tag="JZ382_001375"
     CDS             2694..3872
                     /locus_tag="JZ382_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY2427569.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            3835..4521
                     /locus_tag="JZ382_001376"
     CDS             3835..4521
                     /locus_tag="JZ382_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAY2427570.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            4521..5681
                     /locus_tag="JZ382_001377"
     CDS             4521..5681
                     /locus_tag="JZ382_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY2427571.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            5691..6566
                     /locus_tag="JZ382_001378"
     CDS             5691..6566
                     /locus_tag="JZ382_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427572.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            6563..6874
                     /locus_tag="JZ382_001379"
     CDS             6563..6874
                     /locus_tag="JZ382_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427573.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            6864..7217
                     /locus_tag="JZ382_001380"
     CDS             6864..7217
                     /locus_tag="JZ382_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY2427574.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            7207..7608
                     /locus_tag="JZ382_001381"
     CDS             7207..7608
                     /locus_tag="JZ382_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427575.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            7601..8005
                     /locus_tag="JZ382_001382"
     CDS             7601..8005
                     /locus_tag="JZ382_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427576.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8017..8625
                     /locus_tag="JZ382_001383"
     CDS             8017..8625
                     /locus_tag="JZ382_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY2427577.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            8645..9007
                     /locus_tag="JZ382_001384"
     CDS             8645..9007
                     /locus_tag="JZ382_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427578.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9010..9192
                     /locus_tag="JZ382_001385"
     CDS             9010..9192
                     /locus_tag="JZ382_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427579.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            9209..12628
                     /locus_tag="JZ382_001386"
     CDS             9209..12628
                     /locus_tag="JZ382_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY2427580.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            12679..13416
                     /locus_tag="JZ382_001387"
     CDS             12679..13416
                     /locus_tag="JZ382_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY2427581.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            13426..15717
                     /locus_tag="JZ382_001388"
     CDS             13426..15717
                     /locus_tag="JZ382_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427582.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            15741..>16327
                     /locus_tag="JZ382_001389"
     CDS             15741..>16327
                     /locus_tag="JZ382_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427583.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         5784 a         2402 c         3435 g         4706 t
ORIGIN      
        1 atcgtttggt taagttctat cagtctccta aatggcgcag gcttagagag aaagcgatga
       61 aacgagatca ctatgaatgc caagagtgta gaagactagg taagtatcat agagtagaga
      121 acgttcatca tataaaggaa gtcaaggata gacctgactt agctttagat ttagataatc
      181 ttatttgttt atgtgttgaa catcataatg aagttcatgg cagatatctt acagcattgg
      241 ataaacaaga gaagaagata gaaagctttg ctaacttcga tgcaagtgaa aggtggtaag
      301 tgcatgatca tcaatgataa tggcagagag tatgatacag aaaagattga agagtattca
      361 tcttatactc agggattaat taaacgtttg atatacgttc gctatgtagg tattagggat
      421 ctgttatcag ataactgttg tagtaaatac aaagtgaatc aagtaagaga agcgttgaat
      481 aaagataata acgtcgaaag aataaaaaat gtttttggat atagtattga agagattaat
      541 tattacattg acttcgctga agctttcatt ccgatggtga gataaccccc ccttaaaata
      601 aatcgcaaat tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc
      661 gaactttatc atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac
      721 agagaaaaat tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag
      781 ccattcttag ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa
      841 gaagatatat ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg
      901 ttgcttgttt ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct
      961 gaattggtag gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt
     1021 caaagctata gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta
     1081 tctagaagaa agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga
     1141 ttcaatcaat ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct
     1201 acaatgtttt atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata
     1261 tctttcatta atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt
     1321 tctactaacc gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga
     1381 aagccaagca aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt
     1441 agataaaaaa gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa
     1501 acttcgttat aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg
     1561 ctttgatgaa attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg
     1621 tggtaaaatt cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg
     1681 tccgcttgat gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga
     1741 caaggatgga gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa
     1801 tgaagttgat gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaaa
     1861 tttaaaacgg aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact
     1921 tacgttcatg accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc
     1981 atatgatgat gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat
     2041 aggaacagtc gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata
     2101 cgataaagat gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca
     2161 aaacatcaaa cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca
     2221 tggaaaaaat atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata
     2281 ttatattaaa aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga
     2341 agaagcaggt tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa
     2401 agatctggtt gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat
     2461 gcgttggtat tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga
     2521 aaaaatagaa cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa
     2581 ttttttagat gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa
     2641 tacaggaact ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga
     2701 tttttcaaaa ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact
     2761 tttgtaaatt gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt
     2821 tgattgcaaa tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa
     2881 agaatgatct ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat
     2941 ttagaaaaaa actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt
     3001 ctaataattc tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga
     3061 aagatgatgt gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag
     3121 aaagtgatgt tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt
     3181 attatcaagc gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg
     3241 ctcgtagata cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc
     3301 aaatcaataa aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg
     3361 cggtatttca attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca
     3421 attcaagaga tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc
     3481 acgttccgaa aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt
     3541 ttttaatgtt cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc
     3601 tctatgaagt agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca
     3661 agattactag cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat
     3721 ttacaatcaa cgaaggaaga gaacgcgttg gaaatgatcg ctctgataag gcgatggcag
     3781 atgaaatatt tataactaaa aacaatcaac aagtatcgaa aggaggtgag gcgaatgacg
     3841 acaatgaaaa catttctagc agtaaagaat gaaggcgcag taccgcaaat ttttattcag
     3901 ggatttattg gttctagttg gttctttgaa gggaatactg acaagggaat caaaaatatt
     3961 ttggatagtc taggtgatca agaagaaatt gaagtagtaa ttaattcaaa cggtggagac
     4021 gtatttcaag ggattgctat tgggaactta cttaagtcaa ataaagcaaa agttaacgtt
     4081 gtgattaacg gcttagccgc tagtgctgct tcaattatcg caatggctgg cgatactata
     4141 aaaatctaca acaatgcaca attgatgatt caccgcgctt ccacatacgg agaaggaaat
     4201 gtcgatgact tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc ggtaaaggct
     4261 tcatataaaa cacgattcaa tggcacagat gaagcattgc aagaacttct tgaaaaagaa
     4321 tcgtttatgg atgcagaaac agctttgagt tatggattgg tcgatgaaat tatcgatgca
     4381 gaaaatagct caggtactga agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac
     4441 gttaaagaaa aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt
     4501 ggacaaggag atgtaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg
     4561 accaattgat gaaagctttt aaagatggta acgaagaatc ttttagcgca gctatggtaa
     4621 gcttatctaa ggaaattcag gataaaattt tagaagaagc aacagcaaaa aatcaagatc
     4681 aattagtatt aatgaaccgt ggtcagcgtg tattaactac acaagaaaca aaattctata
     4741 acgaagtagt gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca gctactgtat
     4801 ttgaacgtgt atttgaagat ttagaacaat ctcatccact attgcaaaaa attacttttg
     4861 ttaacacaac tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca gcatggtggg
     4921 gtaaactttg cgaagctgtt aaaaaagttt tagataatgg ctttgatgta attaacatga
     4981 agcagttcaa gctatcaggt tatattcctg tatgtaaggc aatgcttgat ttaggtccag
     5041 tatggttaga tcgttatgtc cgtactgttt tagtagaatc attgagaatt gcattagaac
     5101 aagcaattgt tgatggtact ggtaaagata tgccagtcgg aatgatgcgt gacatgagca
     5161 aacaaactag cggagaatat gctgaaaaaa aagcagaacc tattacagct ttagatgctg
     5221 taactatggg ccgtttgatg gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc
     5281 cgatttatcg taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact
     5341 ggtctaaagt attcccagct aagactgtac taactgctaa tggagaatac gtacaagtat
     5401 tgccagtacc agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg
     5461 gggtagcctc agattacttc atgggtgtag gatctacact aaaaattgaa gcttcagatg
     5521 aataccattt tgttgaagac gaacgcattt atctagctaa acaatatgca aacggtcaac
     5581 ctaaacgtaa cgatagtttc attgtattag atattagcgc tttgggaact actactacaa
     5641 ctacaaaacc aacaaccaca acaactacaa cacaagcgta ggtgatcaga atgaagtata
     5701 ttctttgtca gccggcaatc aatcggttta aatgggagct tgaagtttgt ttaactaatc
     5761 tgaagaaact aggaatcaaa gatatcgtat tgcttttcag cagacacgat gatcagattc
     5821 ctattttttt tgagaaggaa tatggtgttg aagttcatgt gtacgatgat ctgcgggacg
     5881 acaaagagta tattccttcg attaaaccat atttatggtg gaaatattta gaagaagatc
     5941 attcgcgtga ggacgaccga tatttctata tcgattcgga tgtcattttc aataaaagaa
     6001 ttaatttgcg caaattgcct tctaaagatg atgtttggta ttgtagcgac tgctgtagtt
     6061 atctaagtct tgattatatt agaagctgtg aaaacggaga aaatattcta aaagatatgg
     6121 caaacattgt aaatgttaca gtagaatctt tggaaactat aaacactaat tcaggaggcg
     6181 cacagtgggt tattaaccgt cctaaagcga attattggaa aaaggtttat ctggattcta
     6241 atcggctata tcgctacctt agagggcaaa aaacaaatat acaaatctgg acagccgaga
     6301 tgtgggcacg gctttggaac atgatgtatt tcaatattgg tcctaaagtt cacgaggaat
     6361 tagacttttg ttttgctact gatccaatag aaaaagttaa agaagtaaaa atcttacaca
     6421 atgctggagt aacaacaaac gatgaagatt tatttttcaa agggagatac gtgacttcta
     6481 cgccttttga tgaagattta tcatttgtaa acaagaaaaa atgctcttac gcatatgtta
     6541 aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg actgaagaat tgctaatagc
     6601 tgtgaaggat aatatttacg ttacctggaa cgaagaagat gagtcaatta aaaagatgat
     6661 agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca acactttctt tttctcctga
     6721 aagcttagaa tacggattgc taatcgaaag atgtagatac gactggaatc gtgctttaga
     6781 tgagtttgaa caaaatttcg ctagtgagtt attaggtttc attcaacatt atgcgctaca
     6841 agaatatatt gcaggtgatg tgaatggcga ataatcgtag actcgaagaa acattcaacg
     6901 atggttggtt aaagattttg acgcaaacta ccaaaagaaa tgaactagga aaaaagattg
     6961 gtgtagaaga tacagaaatc acttctttaa aatttagaaa tctttccatg agagatagtg
     7021 atataacagc tatggatgcg atgggatcga aattaactaa gaaagtaaag actccatttc
     7081 atccaatcgc caagaaattt aataaagatc aatattttat cgtaatcgat agtatgcgtt
     7141 acaacgttat ctatgccgat tacgataatt tttatatcta tttttatctt gaaagtgtgg
     7201 gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt taaataagca aatttctgct
     7261 atcaaaactt ccttagaaga gcattttggc ctcaaactct ttcaagactc cgtaggcgag
     7321 gatgagctac ctgatgattt taattacttc attctcgaaa caggagaaat agaaatgatc
     7381 actgagccaa aatatagcgt gggtcaaaat ctatatctaa ctttctattc agaaaataga
     7441 gaagatttaa caggagactc actagatatt atttcattga ttcaaaatcg ttcgattcgt
     7501 tttcagagaa tggatcccaa tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa
     7561 ttggtattta cgtttagacg attattgaag agtgattgtc atggctaaaa atagttggga
     7621 gctaaaaata aatggacatg atgaacttct tgtgcggatg gaacgctatt caagcgagag
     7681 cgaacgactg attaacgaag cattgaaatc aaaaggttcg gctattgcag tggataggat
     7741 tacagaaaaa attcctgttt ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa
     7801 tagtcgtcca cttaagactc aatacattaa tttgggtttc atcattagac ctacaagaaa
     7861 atttgagtat ttaaaatatc ctgatttggg gataggtact tctaaaagaa atcagccgga
     7921 cgaatttatg agaagaggat taggtcttgc acttgatcca attacagaac ttctgattcg
     7981 tcaattcgat aaattaaata aataggggga acaacaatgg ctaaaacaaa aactgtagta
     8041 acaacgttcg ataacgtaag tatcaaacga attgctttta attttaagaa cgcaggaaat
     8101 gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa cagaaatgca aacggtggtt
     8161 aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac caatcaatat gacggtaaca
     8221 attactgcac atgtaccgat ggaagtttat cgacgtttca atgggttgaa acaagatgga
     8281 cgtattaaac caggcattta ctcttacggt cctgattccg taggcgaaga tttctcactt
     8341 gctgcagaga tcgtggatga cttcgaagaa aatagaaagt tagttggtat gttagcatgc
     8401 acttcgaata caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta
     8461 gaactagaaa caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt
     8521 gcagaacttg aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt
     8581 aaaaaaactt cagttgtgac aactacggcc actcaatcac agtaaaaaaa acggaggtag
     8641 cgaaatgaac gaagattact caaaaattga actaaacgat ggaacaattt tgaatttaga
     8701 acctaaactg aatatcaaga aattattgat gatcaataga gattttaaca cagacgagtt
     8761 tgcaaaaatg actgtgggaa aaggatccat ggatatttct gttattcaag gtgcaaaggc
     8821 tgtgtatatt gcttaccgcc aagcgaacat gactgattat atttcattcg atgaatttat
     8881 cgataaatgg gattttgata tggctactgc cagctatatt tatcaattga tgatgttcaa
     8941 acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca aataaggaaa aaaagcttca
     9001 aaagtaaaaa tgccaaagct cttagttgaa acgtgggtcg atgtctattc gatgttgacc
     9061 gacgtttttt ctatgccttc agatttggtt ttaagcgata tctgtttaga tgacattttg
     9121 caaatggctt acaacaagag tgcttatgaa ggatggaaaa actatgcaat aaaccaatct
     9181 cagaaaaact aaagaaagga ggtaaaaaat ggctaaaaag agaacagaag cagaagtaac
     9241 tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac
     9301 taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc
     9361 agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagccaaaa
     9421 agttgaagta actagccaac gtttagccaa tgccaaaaaa tattatggag aaaattccac
     9481 cgaagttcag aaacttgaga gagaactgat taaccaacaa acagcgcaac aacgtttgtc
     9541 aaacgaaatt gataaaacga gtaatacact agctcaagca aaaggcgaaa tacagacgta
     9601 cgagtctaca atgcaacagt tggatagtga acaaaaaaat gttcaagcta gtgcttctct
     9661 gattgaatcc gaatacaaaa aatggcaagt aactgctggt caatcagctt ctgaatccga
     9721 gaaattagcg aaagcccaag aatatgtttc tcaacaatct gaaaatgcag agaaaacgat
     9781 agatatcctg agacgacagt tagaagctac acagtctgag tttggcgcta catccacaga
     9841 agcaatgcag atggaggcga agcttaatga tgctgaacgt gaatttgaag agttaggaca
     9901 agctgctaaa aatgtagata caactaactt ggacgatatc ggaagcaaaa tagatatgaa
     9961 taatttaatg gaagcttctg acgttttaag cgacattggc gataagctta cagaattagg
    10021 gaaacaagca gtggactctg ctaacagtgt aggtagttcc cagagtaaaa tacaagctaa
    10081 ttttggtttg actaaacaag aggctgaaga attaacgaat gtagccagag acatttatta
    10141 taaaggtttt ggagaatcgt tagatcagtc cacagatgca ttgattttgg taaagcgtaa
    10201 tttaggcgat ttaaataatc aagatttaca aaatattacg gaacaagcta tggtcctaga
    10261 aaacaccatg ggcgctgata tggatgaaac gttacgtggt gtaaatggct taatggtcaa
    10321 cttcggattg agcgctcaag atgcaatgga tttaatggtt tcgggtactc aaaacggttt
    10381 agataaaacg cacgaattag gcgacaatat ggcagaatat agccaattat ggagtcaaat
    10441 gggatattca gctgatgaaa cgttcggaat gcttcaaaat ggtttagatg cgggtgctta
    10501 taaccttgat aaagtcaatg acttagttaa ggaaatggga atatcgttaa cagatggtcg
    10561 atttgagcaa aacatggata tgtttagtga aagtactaga aaagcttttg aagagtggaa
    10621 aaatggtgga ggaacacaaa aagacgttat taattccatg attcaagatt ttagcaatat
    10681 ggatggtcaa tacgaccaat taaataaagc ttctacaatt tggtctgcac ttggcgaaga
    10741 taacgcgatg aaagttgtcc aatctttgac tgatgttaac catacatttg atgatgttag
    10801 tggatctgca caaaaaatga atgaagattc tactactccg ttacaagaat taaacggaaa
    10861 aatagctgaa ttaaaggatt cattagctcc tataggcaac acaatcatag atgcactcga
    10921 accagtaatt gattttctag gaaagatggc tgatgcgttt aataatcttc cacaaccagt
    10981 acaggattat gccgtagcga ttggcggatt gactgctgca tttactttat taatgccaat
    11041 aatagttggc ttcatggctc taggtggtcc tactacatta ataataggag cagttattac
    11101 tgctattgct ggagttatag caattataaa aaactggggc gcaattactg actggtttaa
    11161 gggaatatgg agtaaattca ctgattggtt gggtggtact tgggaaagca taaaagaagg
    11221 tgcctcatca gtttgggatg gagtcaaaga aacctggtct ggatttgtag attgggttca
    11281 agaaatttgg caaggagttt ctgattggtt tggagagtta tggagcggat tagttgaagg
    11341 agcttccaac atctggcaag gagtccaaga gacttggcaa gcattcgttg attgggtttc
    11401 aaatatttgg aacggagtca aagaagtatg gtcgattatt tgggcagaca ttgtaggaat
    11461 tgttcaaata ccatggacat taataacgtc attgattcaa gctggtatta atattatcgt
    11521 gggtatcttt gatgtagctg gacagttatt aggcgcagct tggcaagctg tttggacacc
    11581 tatttctgat ttccttaaaa atacttggga tactatgaca caatggataa gcatcgcttg
    11641 gaatggaatt gtaactacat tccatactat atttgatcca gtagtggcat ggtggaatgg
    11701 tatatggaca tctattagta ctacggcttc aaatatttgg aactcaatta gtgcaacagc
    11761 ttctagtatt tggaatagta tcaagaatac aatcactagc ttggtacaag cagctgctac
    11821 agtaattcaa aatatttggt caactgtatc tagttggtta ggtggaattt ggaattcaat
    11881 cagctctaca gcatcaaata tctggagtag cgtgactagt agtataagca atgctataaa
    11941 cgcagctaaa agtgccattc aaagtgtttg gaatagtata tcttcgtgga tcagcggaat
    12001 ttggaacggt atcaaaaaca ctgctttgaa tctttggaat ggaattacaa gcactattag
    12061 ctctaaagta aacgatggaa aaaatgcaat ttcaagcggt tggtccaatc taacaggtat
    12121 tgtttccgac atattcaata atgttaaaag cacgatagct aatatatggg aaggtatcaa
    12181 aaagactgtt agcgctccga ttgattggat tagagataaa atcagtagtg tctttgataa
    12241 tttgaatatt tctataccac atattccgtt accacatttt aaattgagcg gagaattcaa
    12301 tccattgaag gggaaaatcc caacattggg tgttgattgg tatgcgaaag gtagtgtgtt
    12361 taattctcca aatattatcg gtgtaggtga atcaggacct gaagcagttt tacctttgaa
    12421 aagatctgtg ctgcaagaaa ttggtgatcg tatcttgagt agcacctcag tttcatctag
    12481 gacacaagcg gttcaacctg tgaacaatta cgaattcaat ttcacaattg atggtaacgc
    12541 agatgaagtc actatgaagc aaacaactca acaaatcatt gatagcatta caaaagttca
    12601 aaatgataat gcttcggcat ggcgttaaac aggagagtat ttctcctgtt tttttagtat
    12661 taaaaaggat gtgaaaaaat gactgattgt atacattcta taatcgatgg atttcctgat
    12721 tatttgcata aattggcttt agctgaaaga ccaaccatac cttctccaaa aagacagaga
    12781 gttgaaactt ctgttttagg aaggttaggt ggcttagtgc aagattactc gtttgaagac
    12841 atgtcgttta cattgcacta taactattta gaggatgtgg aagaccatca agcgttcaag
    12901 caatcgtttt atatcatgcg tcattggtta aactatgcaa agaaattaga attctctgac
    12961 gatcccaacg tctattacgt tatccagact atcgatattg gggatgcaga aaacgatatt
    13021 gttgaatggg gagagttcga tgtaaatatt actgcgaaac cattcgcaag agttcaagaa
    13081 gatgtaccta taaccgtaga taaaccacag tcatttaact tgctgaataa tagtttagaa
    13141 gaaagttttc caaagattat catcactcct tcagctactt catgccagtt catcttaaat
    13201 gattatgtgt ttagtttcga aggcttagta gcaggaactg atgtagtcat tgatagtgat
    13261 ttgatgcttt gctacgaaga gcaatcggac ggagatattt tagatcggtc caacaaaatg
    13321 aagaccatgc aatatccgac attgcaagtg gatatcaatc attttaattg cactggtttg
    13381 agcaaaatac aaatttatcg taacgggtta aggtaggtga aatagatgat cgataattta
    13441 ataactattt acgataaaaa tgacgcgaat aatttagctg aacatttata tgatacgcaa
    13501 ggtttaggcg ctttgtcaga ctggttaaca gctactgtta gcaataaact aaacggagcc
    13561 gagatatttc agggtactta tccaataagc ggaactaatg cagatttgat tgtagaagga
    13621 cgtattattc agtgttatgt agatgaaaat cgagcaaaac agcgtctacg gatttattat
    13681 gcaaaaactt ctgtaatagg caatacgata gaagtaaaag ctgaacctat tttcaatgat
    13741 ataagaaaat cggtgttgaa taaatatgac agcggaacag aaaaaatcac tgctactcag
    13801 gcatggcaaa acgcaaaagc tttagcgaaa ccagctatcc cttcgcagtt ttctttctcg
    13861 tcattagtag atacgcttgc taatgtgaag atagaaaaag cgaatttttt agaattcttt
    13921 ggtggaaaag agggatctat tctagatcga tttcatgggg aatttcttaa agataataac
    13981 acattacatc atgaaaaaag gctaggtacg gatcataaaa tcaaagcgat ttatactaaa
    14041 aatttaactg gtcttgactt agagatagat gctcaaagcg ttttagttgg agtttatcca
    14101 ttcattagca gttcttcaga aggagaagac gagatcactc taccagaaga agtcattttc
    14161 acggattacg tggatgatta tcctgctgga tatgtttctt ttgttgattt taaagacaaa
    14221 gcgactgatg tagccacatt aagggaagct gctaaagact ggttgaaaac aaacatagat
    14281 aaacaaaaac cacaagtgag tggttcgatt gaattagtac cattgaggca tcaaagaggc
    14341 tatgaaaaat ttgttgatct agaaaaagtt tcgatgggtg acggagtaga tgtgtatcat
    14401 ccacagttaa aagtgaatat gtcagcgaga attgtggaat atacgtttaa tgttctaacc
    14461 aattcatacg ataaattagt tgtaggaaac gtcaaaacaa acttcttaga aaacacagag
    14521 aataatgtaa gcaatttgat taatgatgcc attgatcaat tgaaaaatgg tggcgaaatc
    14581 agtgatttaa tcaatgatat tgtagatcat caaactgata tgattactgg tcaaaatggt
    14641 ggttatgttt tattagatcc taaagaagcg cctagtcgta ttttgattat ggacacacca
    14701 gataagaata ccgcaaggaa tgttttacaa atcaacaatg ctggtattgg tttttctaaa
    14761 actggcatta atggaacata tgaaaccgca tggacattag atggcggatt caatgcctcg
    14821 tttattacgg ctggtgaaat agtaggaatt actattagag gtactacatt aattagtgat
    14881 ggcactgatt atagaacaag tattgctaat ggcaaaatga cttggtactc aaaaaaagtt
    14941 aacaaagata ttatggagct agaagcacgt gattatgtaa gtgctgatgc cggtattgta
    15001 tcatacacca tgaaaactgg tggtggtttc atgattagaa atccacaggg taacttggtt
    15061 tttagtacgt gggataatgg taataacaga ccgtttctat cttttggtgc gcccaatttc
    15121 aggtatagca atgctagtta tgtaacttct ggcgacggta gttctttaag cattaatggt
    15181 agtgcgggta actcatggga atttaaggta gctggtagga ctatgaaatt tactagtgat
    15241 ggtatgctaa cgttgccagg ttgttttttt ggttcatggg aagatgggaa acttgctagg
    15301 tttgaacaat caacggtaca agtatataaa gattttactg ttagaggtac taaaaactca
    15361 accgtaccaa cagaacatta tggacaacga ctattaaacg cttatgaaac tccagaatat
    15421 tatttcgctg attatgggga agccgttaca ggtgatgatg gtaaagttcg tgttgatatt
    15481 gaccccatgt ttgctgagac agtaaatcta agtcgatata tgacacatgt gacacctaca
    15541 gaactagttt tgtgtgctgt tactcatgaa gatattgacc atttcatcat tgaaactagt
    15601 aagccaaacg tattagttag atggaattta gtggcacacc gtctagggta tgaagatatt
    15661 agattaaaag aggatacagc atatgatagc acagtgcttg accaaaaacg tttttaaaac
    15721 gaagacaagg aggtatataa atggctagca gtttatataa tttggctcta gatttcagca
    15781 aagaattaaa ctacaccaaa gctattatgg ctcgtcaagg tgataaaggg attacagtga
    15841 ctgttaaacc atttttaaat ggcttgcaga tggatacgag tggcggaaca tttactttaa
    15901 aaggaacaac gccatctaac cgttacgtag atagtgtggc aactagtgta actagtgaag
    15961 aagtcacgtt ttctcttgat ggcacattta tgagtgaagc aggatattat aaacactgct
    16021 acgtagaata tagaaaagac aatcaaattt taacaacgca agatatcatt tttttctcac
    16081 taggagtgtc tgacatttcg caaggccaag ccgatgaata cgtttcgcaa ttggaagagt
    16141 tgattcaaaa gtataacgaa acttttgatg cttttatggc tgaaatcgaa ggtagagtgg
    16201 acagcttaaa taaacagatt actgatttaa ctggtcaagc taaaacacta caagacaagt
    16261 tagacgctct gaaagaagaa atttctaaat taggtaactt gcaggtgatg tacagtaaca
    16321 gcatcga
//
LOCUS       DADJYG010000055        16237 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.055, whole genome shotgun sequence.
ACCESSION   DADJYG010000055 DADJYG010000000
VERSION     DADJYG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.055"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..642)
                     /locus_tag="JZ382_001390"
     CDS             complement(<1..642)
                     /locus_tag="JZ382_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427584.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYI"
     gene            complement(821..1540)
                     /locus_tag="JZ382_001391"
     CDS             complement(821..1540)
                     /locus_tag="JZ382_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY2427585.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1671..2822)
                     /locus_tag="JZ382_001392"
     CDS             complement(1671..2822)
                     /locus_tag="JZ382_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAY2427586.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3039..3779)
                     /locus_tag="JZ382_001393"
     CDS             complement(3039..3779)
                     /locus_tag="JZ382_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2427587.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3783..4106)
                     /gene="rsfS"
                     /locus_tag="JZ382_001394"
     CDS             complement(3783..4106)
                     /gene="rsfS"
                     /locus_tag="JZ382_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY2427588.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4163..4756)
                     /locus_tag="JZ382_001395"
     CDS             complement(4163..4756)
                     /locus_tag="JZ382_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2427589.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4740..5387)
                     /locus_tag="JZ382_001396"
     CDS             complement(4740..5387)
                     /locus_tag="JZ382_001396"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY2427590.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5400..5711)
                     /gene="yhbY"
                     /locus_tag="JZ382_001397"
     CDS             complement(5400..5711)
                     /gene="yhbY"
                     /locus_tag="JZ382_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY2427591.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5727..6836)
                     /gene="yqeH"
                     /locus_tag="JZ382_001398"
     CDS             complement(5727..6836)
                     /gene="yqeH"
                     /locus_tag="JZ382_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAY2427592.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6833..7360)
                     /locus_tag="JZ382_001399"
     CDS             complement(6833..7360)
                     /locus_tag="JZ382_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAY2427593.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7540..8325)
                     /locus_tag="JZ382_001400"
     CDS             complement(7540..8325)
                     /locus_tag="JZ382_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY2427594.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8318..9187)
                     /locus_tag="JZ382_001401"
     CDS             complement(8318..9187)
                     /locus_tag="JZ382_001401"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAY2427595.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9188..10567)
                     /locus_tag="JZ382_001402"
     CDS             complement(9188..10567)
                     /locus_tag="JZ382_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY2427596.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10575..10994)
                     /gene="fabZ"
                     /locus_tag="JZ382_001403"
     CDS             complement(10575..10994)
                     /gene="fabZ"
                     /locus_tag="JZ382_001403"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY2427597.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11019..11495)
                     /gene="accB"
                     /locus_tag="JZ382_001404"
     CDS             complement(11019..11495)
                     /gene="accB"
                     /locus_tag="JZ382_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY2427598.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11498..12733)
                     /gene="fabF"
                     /locus_tag="JZ382_001405"
     CDS             complement(11498..12733)
                     /gene="fabF"
                     /locus_tag="JZ382_001405"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY2427599.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12752..13489)
                     /gene="fabG"
                     /locus_tag="JZ382_001406"
     CDS             complement(12752..13489)
                     /gene="fabG"
                     /locus_tag="JZ382_001406"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAY2427600.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13501..14418)
                     /gene="fabD"
                     /locus_tag="JZ382_001407"
     CDS             complement(13501..14418)
                     /gene="fabD"
                     /locus_tag="JZ382_001407"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY2427601.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14525..14749)
                     /locus_tag="JZ382_001408"
     CDS             complement(14525..14749)
                     /locus_tag="JZ382_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY2427602.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14802..15764)
                     /locus_tag="JZ382_001409"
     CDS             complement(14802..15764)
                     /locus_tag="JZ382_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY2427603.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15774..16214)
                     /locus_tag="JZ382_001410"
     CDS             complement(15774..16214)
                     /locus_tag="JZ382_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2427604.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         4276 a         3707 c         2784 g         5470 t
ORIGIN      
        1 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
       61 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
      121 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
      181 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
      241 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
      301 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
      361 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
      421 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
      481 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
      541 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
      601 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
      661 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
      721 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
      781 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
      841 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
      901 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
      961 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     1021 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     1081 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     1141 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     1201 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     1261 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     1321 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     1381 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     1441 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     1501 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     1561 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     1621 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     1681 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     1741 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     1801 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     1861 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
     1921 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
     1981 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
     2041 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
     2101 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
     2161 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
     2221 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
     2281 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
     2341 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
     2401 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
     2461 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
     2521 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
     2581 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
     2641 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
     2701 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
     2761 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
     2821 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
     2881 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
     2941 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
     3001 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
     3061 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
     3121 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
     3181 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
     3241 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
     3301 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
     3361 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
     3421 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
     3481 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
     3541 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
     3601 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
     3661 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
     3721 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
     3781 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
     3841 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
     3901 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
     3961 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
     4021 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
     4081 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
     4141 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
     4201 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
     4261 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
     4321 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
     4381 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
     4441 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
     4501 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
     4561 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
     4621 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
     4681 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
     4741 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
     4801 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
     4861 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
     4921 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
     4981 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
     5041 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
     5101 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
     5161 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
     5221 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
     5281 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
     5341 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
     5401 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
     5461 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
     5521 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
     5581 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
     5641 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
     5701 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
     5761 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
     5821 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
     5881 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
     5941 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
     6001 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
     6061 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
     6121 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
     6181 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
     6241 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
     6301 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
     6361 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
     6421 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
     6481 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
     6541 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
     6601 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
     6661 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
     6721 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
     6781 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
     6841 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
     6901 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
     6961 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
     7021 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
     7081 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
     7141 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
     7201 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
     7261 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
     7321 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
     7381 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
     7441 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
     7501 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
     7561 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
     7621 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
     7681 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
     7741 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
     7801 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
     7861 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
     7921 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
     7981 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
     8041 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
     8101 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
     8161 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
     8221 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
     8281 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
     8341 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
     8401 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
     8461 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
     8521 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
     8581 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
     8641 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
     8701 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
     8761 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
     8821 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
     8881 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
     8941 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
     9001 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
     9061 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
     9121 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
     9181 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
     9241 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
     9301 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
     9361 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
     9421 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
     9481 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
     9541 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
     9601 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
     9661 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
     9721 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
     9781 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
     9841 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
     9901 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
     9961 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    10021 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    10081 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    10141 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    10201 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    10261 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    10321 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    10381 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    10441 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    10501 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    10561 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    10621 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    10681 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    10741 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    10801 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    10861 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    10921 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    10981 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    11041 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    11101 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    11161 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    11221 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    11281 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    11341 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    11401 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    11461 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    11521 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    11581 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    11641 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    11701 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    11761 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    11821 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    11881 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    11941 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    12001 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    12061 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    12121 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    12181 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    12241 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    12301 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    12361 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    12421 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    12481 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    12541 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    12601 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    12661 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    12721 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    12781 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    12841 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    12901 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    12961 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    13021 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    13081 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    13141 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    13201 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    13261 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    13321 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    13381 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    13441 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    13501 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    13561 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    13621 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    13681 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    13741 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    13801 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    13861 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    13921 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    13981 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    14041 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    14101 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    14161 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    14221 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    14281 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    14341 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    14401 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    14461 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    14521 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    14581 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    14641 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    14701 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    14761 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    14821 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    14881 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    14941 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    15001 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    15061 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    15121 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    15181 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    15241 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    15301 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    15361 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    15421 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    15481 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    15541 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    15601 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    15661 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    15721 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    15781 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    15841 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    15901 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    15961 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    16021 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    16081 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    16141 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    16201 aaatctggtt ccatgttcca ccgtccacta attagtt
//
LOCUS       DADJYG010000056        16197 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.056, whole genome shotgun sequence.
ACCESSION   DADJYG010000056 DADJYG010000000
VERSION     DADJYG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.056"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..254
                     /locus_tag="JZ382_001411"
     CDS             <1..254
                     /locus_tag="JZ382_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY2427605.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
     gene            443..1111
                     /locus_tag="JZ382_001412"
     CDS             443..1111
                     /locus_tag="JZ382_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAY2427606.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1311..1541)
                     /locus_tag="JZ382_001413"
     CDS             complement(1311..1541)
                     /locus_tag="JZ382_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427607.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1668..3050)
                     /locus_tag="JZ382_001414"
     CDS             complement(1668..3050)
                     /locus_tag="JZ382_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY2427608.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(3206..4393)
                     /locus_tag="JZ382_001415"
     CDS             complement(3206..4393)
                     /locus_tag="JZ382_001415"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY2427609.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4400..5431)
                     /locus_tag="JZ382_001416"
     CDS             complement(4400..5431)
                     /locus_tag="JZ382_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAY2427610.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5447..6901)
                     /locus_tag="JZ382_001417"
     CDS             complement(5447..6901)
                     /locus_tag="JZ382_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2427611.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            7076..7981
                     /locus_tag="JZ382_001418"
     CDS             7076..7981
                     /locus_tag="JZ382_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY2427612.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            8072..8749
                     /locus_tag="JZ382_001419"
     CDS             8072..8749
                     /locus_tag="JZ382_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAY2427613.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            9030..9926
                     /locus_tag="JZ382_001420"
     CDS             9030..9926
                     /locus_tag="JZ382_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2427614.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9981..11351)
                     /locus_tag="JZ382_001421"
     CDS             complement(9981..11351)
                     /locus_tag="JZ382_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2427615.1"
                     /translation="MEEKKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11589..12335
                     /locus_tag="JZ382_001422"
     CDS             11589..12335
                     /locus_tag="JZ382_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAY2427616.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12332..13171
                     /locus_tag="JZ382_001423"
     CDS             12332..13171
                     /locus_tag="JZ382_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAY2427617.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            13172..14122
                     /locus_tag="JZ382_001424"
     CDS             13172..14122
                     /locus_tag="JZ382_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAY2427618.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14263..15015
                     /locus_tag="JZ382_001425"
     CDS             14263..15015
                     /locus_tag="JZ382_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427619.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            15034..16185
                     /gene="queG"
                     /locus_tag="JZ382_001426"
     CDS             15034..16185
                     /gene="queG"
                     /locus_tag="JZ382_001426"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY2427620.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5271 a         3029 c         3260 g         4637 t
ORIGIN      
        1 gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg aactgcttag
       61 ataattcact tatggagaat ttttttggct tattaaaaca agagattttt cacgggaaag
      121 tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat tactacaata
      181 atgaacggat aaaacagaaa ttaaactggc aaagtctcgt ccaatttcgt aaaacaaccg
      241 taacggttgt gtaaaaatat cattttagag cataaaaaaa aggagtggat ttctccactc
      301 caaaaaagtc taacttttgg gggtcactac agtgtcacag tccttttttt agtaaaagga
      361 tacatttctt ccttgtatat tccatgcgtg ttcgtgagaa agcatatata atttaattaa
      421 cagatagtta gggggagaaa aaatgatttt ttcaattaca atgaaactgg tgttcgggtt
      481 gatcggttta ttattagtgg tacgtttgct tgggaagaaa tccatgtcag aaatcacacc
      541 gtttgattta gtctatacgc ttgttttagg tgggatctta gaagaatcaa cctatgatga
      601 caatgtccat gtagggcatg tcctatttgc cattgcgctt tgggcagtaa tgatttatgg
      661 tatcgaacgt atcgtccaaa aaaatgaaaa ggtcaatcgt tgggtcaaag gcgagccatc
      721 tgttctaatc aaagacggcg tcatcaacat gacagagttg acaaataacc atatcgagat
      781 ggagcagttg cgagcaatct taagacagca agaatgcttt tctcttgaaa atgccaaaca
      841 tgttatccta gagaatgccg gtcaaatgag tgtattgaaa aaatcagatg aagacaaagc
      901 tttatcgatc ctgcttgtag atgaagggca aattcagcat aaagtgcttc agtccaatca
      961 attgactgaa gcatggctta tggagaactt gaagaaagaa ggctatgcag atgtaaaaca
     1021 gctgatttat gttgaatggt cagaagaaaa agggctctac atactaaata aagaagacgt
     1081 catgcatgaa tcttaccgaa tagatggata gatagacaga ggttgtgaaa tcagcgcctt
     1141 ggcttgttac tcaaaactaa gcgacttttt cacaacctct gatatctaat atacgttgtt
     1201 ccagaagttg cttcttcgaa aaataagcca aaattccaca aaaatttaag aagcaatttt
     1261 cgaaaaatcc tttcttattt tttgaagttg atcacttctg tcacaacctc tcatgtcaca
     1321 aaccattttt ttgtttcctt taactcaata taccgtacgt cttcccacaa tatcggctga
     1381 tggtcaatca taaagtaatc ttcataggct tcaccaatga agtgcccttc gacctcatcc
     1441 aagagtcttc caaattcatc ttttaaattg agctgaatga ttactgcttt acgagataaa
     1501 aaggcttctg tcagtatact atcgatttct tgcaatgtca tttgttgttt aggaagaggc
     1561 tgtttgcgtg cttcttcttc atttttttga taccgttgac tagttcttcc atcgcaaaag
     1621 cagtccccca ttttaatcca aatggccgat ctcgatactc gttatagtca atgaattttt
     1681 ttgctgtccg tctcatcttt tccaccttct aatccactca taccacctgc atgcccgcca
     1741 acgagagaac tacgtgcgat ggcgcgtcct ccttgcaaga gactatgcgc atggatgatt
     1801 gattgaaagc cgtatttttt tctaatcgta tcgacaacaa aatcaacttt cgcttcattt
     1861 atctgctttt caggatcttc aaaaaggtta agctgtaaac ctgtcgcgta tacgaggtcg
     1921 gaacagttca cgcctacacg ccgaatatcc tggtagtggt aatgctggtg gaaaatggtc
     1981 aataaagcat ctgctagatc tctgctagca tttgtcgctt cgattttcat ttgctggtga
     2041 aacccggtct tgccatttgg atcaagatag cctaaagagt atcctaccca taagctgact
     2101 acttgtgttt ttgctgcaga tcgtctcaag cgagtgccga cttggtcggc catttctttg
     2161 ataacgatct cgatttcttc tcgtcttgta taatctctat ctaggatttg actatttccg
     2221 atactttttg attttggtgt atattcttgt cccaaaaaac ttcgatcgat gccccaagca
     2281 tgggcataaa gctgagtacc gatgacccct agcttgcttt tcaagtgata ataatccgcg
     2341 tgagctaaat cataaacaga atgtataccc atttgtttca gacgttttgc tgttcgtttg
     2401 ccgattcccc aaaagtcagt gatatctgag attttccata acttttctgg tacatcttca
     2461 taacgccatt cagcaaacat ttgctttccg tcttttgagc cattgtctaa ggctagttta
     2521 gcaagtagcg ggttatcgcc aattcctata gtcgtgtaga ttccggtctg ctcaaacaca
     2581 tctttatgga tcagtttagc catgtcgtac gcatttgttt taccaaagag tgccatgcta
     2641 tttgttacat caaggaagct ttcatcgacg gaatagacat gatggttcgc ttcgtctgca
     2701 tattttttat agatagaatt gatttctctg ttcttttcca tataataggc catccttggg
     2761 gcaacaatat ataaatcact tggatagggg aagggaagat ctctggcacg gctgatattg
     2821 gtgatgccgt aagctttttt tgctgcagga ctggaagcca agattaatcc gctaccccgt
     2881 gtttctgtcg aatcggacgg ataggacatg actactaatt tagttgtaag gggatcaaga
     2941 tccttttcaa cgcactctac ggaggcgtaa aaagatttgc agtcaataca taaaatatct
     3001 cttgaaggtt ctttttcata aacaaatacc ggattttgat tcgcccacat aacaatcacc
     3061 tcttcaacac aattagaaca cacgttctct ttccgtgtca ataaaaaaca gcaattttca
     3121 aatacgagca aaaggctcac atttggaaat tgctgagagg ttatttttat ctatttgttt
     3181 ttagctgaga tcgttgattt ttttatcaaa catgaacagc ttctggatcg atctttgttg
     3241 tcccatttcc aatgtgtgca acataaaatt ccggagcata gccgacaact ttcatgtatt
     3301 catcatagac attgtttttg aaggcaggta tattttcttc tttgacaaga gcaatcgcac
     3361 agccaccaaa tccagctcct gtcattcgtg cacctaagac accttcttgt ttttgagcag
     3421 catctactag tgtatctaat tcgatcccag ttacttcata atcatagcga agagagtggt
     3481 gagaatcatt taacagttga ccaaatgctt ctaagttccc agcttctaat tcagcttttg
     3541 ccttcaaagt ccgctgattt tcagaaacgg catggcgagc tctgcgtatc aatgtatcat
     3601 cgccgatttg gtcaatagtt gattcaaatt cttcttcatc taagtctcct aaagaagaga
     3661 taggcaaagt tttttgcaaa cgtgccaagg cttcttcgca ttcgctacgg cgttcattgt
     3721 attttgaatc tgccaattcc cgccgcttat tcgtattcat gatgacgatt gcataaccat
     3781 ctagtttcac tggaaccatt tcgtatttca gcgtatttgt atctaataag attgcatgat
     3841 ctagctcacc gaagccgatg gcaaactgat ccatgatacc tgaattgaca ccgatgaatt
     3901 cattttctac ttttttacct gtttggacta gttccaagcg atctgttttt aaatcaaaca
     3961 gatcttctaa tacaacacct actaataatt ctaatgaagc gctactggaa agtcctgcac
     4021 cattagggat tgttccttcc actaacaact caaacccatg atcgattgag tgtccagctt
     4081 cttttaattt aaggaccatt ccttttacgt aatttgccca gtttgctttt ttatcatatt
     4141 ctagctcatc taaagtaaaa ctaatgacac cgatatcgtc aaagtttgtg gaataaacta
     4201 atattttatt atcgtctcga ggtgctgcta caccttgcgt tccataagtg atggaagcgg
     4261 ggaagacatg gcctccattg taatctgtgt gttcaccaat caaattgatg cgtcctggtg
     4321 agaagtattg tgtagttttt ttaggtccga atgtttcggc aaattttgtg tttaagcttc
     4381 ttttgatttc catgatgagt tattcctttc ttgttttgat tttgaattct gtcactgttt
     4441 caaatactga tccggctttt aaatgaatcg aaccaaatgc tgagaagcgc tctgcacctg
     4501 gagctgtttg agtctcaaat gtgatgccac cgtgatgtgc cagttttcgg ttgcgcattt
     4561 ctggtgtttc tgtgccgaaa tttgcagtga aaataaccac actagaagca tctgtagcaa
     4621 tacctacttg gattctttta tcggggctag tcaagcgagc ggcttcttta cccaagccgg
     4681 tttcttttaa gaaaaatgga tgatcgattc cgtcaacaag atttttttga tccaaatcac
     4741 tagcaaaaac ggacgctaat tttttaggta tttgaaaatc gaaagcactt cctgcagcat
     4801 tttcttttac ccctatagga atactatctg ttcgcaaagg agcataagct tcgctattga
     4861 gccaaagctc atgttgatcg attgactgag aagcatctcc cgtcaagttg aagtaaacat
     4921 gatttgtcgg attgaataaa gtatcttggt cactggtgcc gcgagaggtc actcgccaga
     4981 tattatcttt tgtcaaagta tagcggattt ccacatccat tgtacctgga aaaccatgtt
     5041 ctccgtcggg acttgtagta gtaaaaatga cagaagcttc attttcgcca ttcaaaatga
     5101 cataagacca tttttttaat tcaaatccag gagcaccacc atgcagatta tgtccagttt
     5161 ttggatctgt ggctaattgg taagtttttc cattgagaga aaagcggcca ttttcgattc
     5221 tccctgcggt gcgaccgata gaagcaccga tatacggatc tttttcgata tattcttcag
     5281 cggtgtcaaa tcccaaaact aattctcgat catttgattt taaactgacg attctagcac
     5341 ctaaatcaga gatagaaagt gctagcttgt ttttgttctc caaggttatc aagtgataac
     5401 cttggccaaa atctttttct gtaatcttca ttttcaagat cctttcttaa tgggaaggaa
     5461 ctactgcatc gtgaagttca tcttccattg tatcagctgg agccaatttg ttttccaatt
     5521 cctcaacgat ttgtgcatgt tttttctctg tcaatgtaat cttccaagca aaaataatag
     5581 cggagaaaat catcaaagca gcaggagcat agaatgcata agctttgaaa gtcgttacgc
     5641 catgagcagt gatatcagca gcagtagcat taccagtcat cccagaagcg atagcgacaa
     5701 accctacgat cccgttagac aaagcaccag cgattttatc tagtaaaggg cgaattgata
     5761 gagtaacagc ttcgttacgg ataccatttt tccattgacc atattcaaca gagtctgtga
     5821 tcgtcatcaa agcggataag aaaatcaatt gttgagggaa gtagaacaat actagcccga
     5881 tgataacgat tggcaaactt gaacctgcga tcgtaaagaa aaagtaagaa gaaatcatca
     5941 ttgcgatccc tccaaggtaa acgtagcgac gagagatgac gcgtgtaagc attgggaata
     6001 gtggaacagc tactaaacca agtaaggcag caactagacc aacgatccaa aattcagttg
     6061 gttggccaag tacatatttg aagaagtaga ataatacggc agttgttgct acattggcaa
     6121 tcgcataagc gatataagca agggcaagcc acatcaactg atcattttga gcaactgctt
     6181 tgaacacatc tttgatccca gctttttcat tttgctgacg gatcgcagta tcgacttctt
     6241 ttgtgcccaa ggcagtgata atcgcagtac cgcctgaaat aacagcagca atcacaccga
     6301 accagaacca gcctgaagcg ccttcaatat gtttatgagt aacaagatag gtaaagaaag
     6361 taacgatagg gacaacgact agagtagtac catttgcacc tagagaagag ccgaaacgag
     6421 caaacgtagc aaatttttca cgttcctttg tttccgtact caaagctgga atcattgacc
     6481 aaaaagcgat atccttaaat gaataaaaac tatccaataa gatgaaaacg attgcaaata
     6541 atatgataaa taaagtcgta ttatgagtag ctaagccaaa gaaattggta aagattgcta
     6601 ccaagagtac ggaactaaca acgccgccga taactaacca tggtttgaat ttaccgtatt
     6661 ttgtacgagt attatcgatg atgccaccaa tcaaaggatc aaaaataatt tcaactaagc
     6721 ggatacctac aactaagctt gtaactgtag caatcatttt tgcattcgtt gccttatctg
     6781 ttcctgcgaa catctggctt gtcacgaata ccataaaata agtgcttaat gtaacataga
     6841 atgtgtcgtg tccaaaagcg cctaaagcgt aggacacacg ttggatcatt ttgcttttca
     6901 taatctgtat tcctctcctg ttcaatatat aatactaagt aaaccggtta caacgaataa
     6961 caaagatctt ttaatgactt aaacttaaca agaaatcgct tcctttttaa tatatttctg
     7021 tgacttttat ttaacttttt gttgcattat actaaaggag taaggaggga tagacgtgga
     7081 gcaatcgatt tttttaaaaa aggaacatca acaagtagaa aaagaattgt attttgattt
     7141 ttgtggtttc tctaaaacga tgccgtttca ttcttttggt cctgctattc gcaatgatta
     7201 catcctgcat gtcgtgatgg aaggcaaagg atcttatcat gtgaaaaatc aacaattcca
     7261 attgaaaaaa ggtgatctgt tcttgattcg tccaggagac tcgacgtttt acttatcaga
     7321 tggagaagat ccgtggatgt attgctggct ttcatttggt gggagtgctg cagaagaaat
     7381 cattcggcag tcgcttttca gagaagacca atacacgatg gtctcttctg acatacaaaa
     7441 atatatcgac attattttag aatgcatgaa agatacggaa gaaaccttgg aaaatgaatt
     7501 acagttaaca gcattaacct accgtttttt atcagtcctt ttaaaagatg gcggaaaggt
     7561 cagcggtggt gtacaaaagg ttcattcctc tttagctgtt gaaacagtca aatacatcga
     7621 ggcacattat gctgaaaaat tgtctgttga ggaaatcgcc cagcatttag cggtcaatcg
     7681 gagccatttg tcgcgagtat tcaaaaatca tatgggtacc agtatcaagg aatatttgat
     7741 cggggtacgg atcaatcgag gggcgtttct tctttctctg acggatgaat ccgtggaaag
     7801 tattgcctat caagtagggt tcaatagtct agtcgtattt agccgaatgt tcaaaaagag
     7861 taccggagaa acggctacga actatcgtaa acgtatgaaa aacgaaaatt ttaagaatat
     7921 tgctttggat accttgaaaa aacagttgga aaaacaaacc gcagtttctt ggacaacctg
     7981 attagtctgg ttaaaatgac aagcatcggc aaaagaaact tgtggcggtt atgctacact
     8041 gtaaagcgaa gactaaaaag gaggaaaagg gatgcgtaca cttatcatca ttagccatcc
     8101 aacgatcgaa gaatctttaa atcagcaatt tttcaaagaa gcttctgctg gtttaccagc
     8161 tacttggcat catttggaat caagttatcc agacggaaaa atagatatag agactgaact
     8221 acagctcttg gcccagcatg atcggatcat tttccagttt ccgttttact ggtatagttc
     8281 tcctgctcat ttaaaaattt ggcaagataa tgtcttggaa cgagcagcac atgtattaaa
     8341 agggaaagaa ctaggaatcg tattgacaac aggtgtggcg gaaaaagaat accaagctgg
     8401 aggaaaagaa gaatatacga tttccgaatt tttgaggccg tatcagcgaa tcgcaaataa
     8461 attccatatg acctatcttc cgccattcgt tttagcacaa tttatgtatt tatcccagga
     8521 aaaacgttgg gagaagctaa ttgcttatca gcagtatctc tcattggaag gcaaaccatc
     8581 gctgactcag cggattgact ggtttattca aagagtacag gaaaatcaga aaatgcagga
     8641 agaagaatct gaaaaacaag catatatcgt agaagcactg actgaggcaa aagagcagat
     8701 cgaagacttg acctttacac tgcaagaaat gaaaggaact tcattatgac aaatgattgg
     8761 ctgatccaac aattagatga attaagcgaa aaatcagaat atcaagaccg tgcgctctta
     8821 tatgaaataa agaaactgat ggctgagata gacgaacgaa caatgcagct gcaaggagaa
     8881 atcgacggac aatcttggaa tcatctcaac tggtgaaatt ttcttataaa taacaaagat
     8941 aaaaatgtgc acagcgacaa ttatttgcta caataagaat atgaagcgtt tgataacgct
     9001 attatacata aaaattggtg gtgaaaaaaa tgtatgatga aaatgagtgg ggagaaaatg
     9061 ttcggatgac ccgaagaaga cagcaaaaac aggcagctcg taagcggatg atcatgattg
     9121 gggcagcagt tgccgtgttt ttgatcatag caggaggagt tgcgggtcta tattttcatg
     9181 gaaaaaattc gtcagaagcg aaaagtacga ccataacgga aacaacgaca aagaaaacag
     9241 agacgaaaca aacaagtgag tcatcaacta aaacaaaaga gacacaaact tctgaagaaa
     9301 aaacagcaaa tggtttgcca gagcaacttc aagcaatcgt aaatgattat tcgaaaaaga
     9361 tcgaagaaaa gacgcctgca ttgattgaag aataccagac agaaattcag ggaaatcaag
     9421 agggaatagc tggtttatca gcagttgcta accaaaaagc acgtgagctt caagcaatct
     9481 ccgacgaagg gatcagaaaa ttacgtgcag cgtatcaggc agcagaaaat aaagacggtg
     9541 tagacttaga tactttgatc aatcaattat cggctaatta tacgaatcat gtcgctaaga
     9601 tttccgatat ttatttgcag actagcgcct ctcttcaagc agagtcaaca agcacacaag
     9661 acacaacatc ttctgattct gagacagcag agtcggtaga agcttctgaa atgcaagctc
     9721 gaacaacaca agacacaaca gattcctctg aaactaatac caacagcgcg acttcacagg
     9781 ctgcgacaac cgtagttttg gaaggagaag gtccaaatca aatagccgaa cgaactggcg
     9841 taccagtaga aacgatcttg tcgctgaatg ggatgagcat ggagaattat ttcctgactc
     9901 ccggtgagac gctaaaattg aattgataaa agcaataaaa aagaacgaag cttccactaa
     9961 ggtgaagctt cgttcttttt ttattttccg gttctttttt cgcgtacaaa gtaatggatc
    10021 gtcatgtaaa gcaagaaaac gagtccaata atcaatgaga ttctcgtttc aggattgata
    10081 aacatgaaaa tcaatacgat agctaaaaaa gcaagtgtca cataattcgt ataaggagat
    10141 agtggcattt taaaaggatg atcatgcatt ttatctgcgt gcttcttttt gaagcgaatc
    10201 tcgctgatca aaatcactac ccaaggaacc ataccaggca aaatacttga gctgtatacg
    10261 aaaacgaaaa tattttctga ctggttgatg aacattggta gaagataatt taagatcaga
    10321 ccaattaaga ttccacctga gattgctacg acaggataaa aaggcgcacc gtgacgagat
    10381 agtttcagaa attttttaga catctttcca tttatggcaa gtgtatagac catacgactt
    10441 gcgctgaaaa tccctgaatt gcaacctgat aatgccgctg taatcacgac aaaattaatc
    10501 agtcctgcag caaatgtaat gccgacttta gaaaaagttt gtacaaaagg tgaaccaatc
    10561 tcactcaatt gattccacgg atagatactc acaatgataa aaatagcacc aatataaaaa
    10621 atcaagattc gaccgatagt ggactggaca gcttcaatga tcgtcttttt aggattctct
    10681 gcttcaccag cagtcattcc gatcagttcg attccttgat aggaagcaac aacgatcgat
    10741 aatgcaaaga aaaatccttt gacgcctcca gtgaagaatc caccattttt ccaaagattt
    10801 gaaatcccta ctgcctgacc atgattacca aatccaaata agattacccc aagacctgca
    10861 atgatcatca aaatgatcgt tatgactttg atgatcgaaa accaaaattc taattcgcca
    10921 aacgccttga cagaaataag atttgccagc ataagaaaca agacgacaat gattccggga
    10981 ataattgccg gcaaatcagg ccaccaataa ttcatataag atcctacagc aatcacttca
    11041 cttattccta cgacaatata ttgaaaaata ttactccaag cggtcaagta gccagccaca
    11101 ggatggatgt actcgctcgc gtaatttgca aaagaaccag tcgaaggatc aacgtaaagc
    11161 atttcgccta aagcacgcat aatcaaatac aaaaagaatc ctgcaatcgc ataggctccc
    11221 atcactgagg gccctgtcca acgtatggtt gaggaggcac ccataaacag accgacaccg
    11281 atcgttccac ctagtgcgat catttggata tgacgggcac taagacttcg agttaacttt
    11341 ttttcttcca tacaaaaact cctttcgttg ttgagtcaac aagaaaaaat aatcaaacgt
    11401 ttcaacacat gtttttcatt attaggttag ccttattgaa ctcttgatgt gattctacag
    11461 aaaattttca taaaatgcga ggaaaattcg aaaaaaactt tttcatcatt ctacttgaaa
    11521 atgctttcca ttttagttaa actatattta aataaaaagt taggtaaacc taaaaaggag
    11581 tgtaagattt ggaacgtacg gccattgtaa ttcaagacct ttcagccgct tatcaaggga
    11641 aaactgtttt gaaaactatc aacttgacga taaatccaca aaaaattacc ggtattatcg
    11701 gaccaaacgg tgcaggaaaa tcaacattta tgaaatcttt gctagagttg ataccagcta
    11761 ctactggtaa agtcaccttt tctggacagc cggtaaactc agtccgtaaa aaaatagcct
    11821 acgtggaaca gcgaagtgaa ttggatcttt cctttccaat tgatgtatta ggcgttgtac
    11881 ttttaggaac atatccatct ttacgaattg gacaaagacc tgggaaaaaa gaaaaagaac
    11941 gtgcaagaca agctttgaaa aaagtaggga tggaagaata tgccaaaaga cagatcagcg
    12001 aactatcggg tggacagctt cagagagttt ttattgcaag agctctagcc caaggagcag
    12061 aatggatctt tttagatgaa ccattcgtag ggattgatgc gttaagtgaa cgaaagatct
    12121 ttgacatctt gcaggaattg aagaattcag gaaaaacgat tttgatcgtc catcatgatc
    12181 ttcataaagt agaagaatat ttcgatgagg ttattctttt aaataaacag ctgatcgctt
    12241 ccggtccagt acaagaagtc tttacatcga agaaccttca attggcctat ggtgagatca
    12301 tcagacattt agtaaaagga ggagagaaaa aatgatccaa tcctttattg atggattaat
    12361 gaattatcaa ttcctgcaat acgctttggt cacttccatg cttgtaggac ttgcttctgg
    12421 agtcatcggt tcttttatta ttttacgagg gatgtctttg atgggagacg ctatctctca
    12481 cgcagttctt ccaggtgtag ccatttctta tatgtttggt tttagttaca tctttggagc
    12541 cactgctttt gggatgctaa cagcagcagt gattggtttc gtgactcagc ggagccgatt
    12601 gaaaaatgat acagcgattg gaattgtctt tagctccttt tttgcattag ggattatctt
    12661 gatttcatat gcccagagtg cgacagattt gtatcatatt ttatttggga atgtcttagc
    12721 tgttagggaa agcgatttgt tgctgacagc actcgttagt ggaatcgtct tgatatttgt
    12781 ctttttcttt tataaagaat tgaagatcac atcattcgat ccgacaatgg caaaagctta
    12841 tgggctgaac acatcgctga tccattatct tttgatgttc tttttgacat tagtggctgt
    12901 agtcagttta cagacagtag gaacaatctt ggtgattgcc atgttgatca caccagccgc
    12961 cacggcttac ttgctaacga accatttact gaaaatgatc attacagctg caggaatcgg
    13021 tatgctaagt gcagttgtcg gtgtgttttt cagttatagt tacaattggc catcaggagc
    13081 tacgatcgtg ttagcatgta ccgcattttt tatccttgct tttttatttt ctccaacaaa
    13141 aggaatttta tttcagaaag gaagcagata gatgaaaaca aaaaaatcac tctttttaat
    13201 cttggcagtt agtttcttgg tattagccgg ttgcggaaag caggcttctg accaagcgga
    13261 tgaaggaagt aaagaaaagt tgtcagtcgt ggctaccaat tcgatcttgg cggacatggc
    13321 aaaagaggtc ggaacagatc aaatagatat ccacagtatc gtaccagtcg gaacagatcc
    13381 gcatgaatat gaagtactac cagaagacat caaaaaggca agtgatgcag atgttatttt
    13441 atacaacggt ttgaatcttg aaacaggtaa cagctggttc gataacttga tggaaacggc
    13501 taaaaaagaa gaaggaaaag attattttgc agttagcaaa aatgtagaac ctctttattt
    13561 aactagcggt gaagaacata caaaagcaga tccccatgct tggctagacc tatctaacgg
    13621 aataaaatat gtggaggaaa tcgcacgtat attctctgaa aaagatgcag aaaatgcgac
    13681 actctataaa aaaaatgcag aagcatatgt ggaaaaacta aaagaattag atacacaagc
    13741 gaaggaaagt tttgcttcta tcgaagagaa caaaaaatta ttagtaacaa gtgaaggggc
    13801 attcaagtat ttttcacgag catatgattt gccagcagct tatatatggg agatcaatac
    13861 agaaagtcaa ggtacgcctg atcaaatgaa agcgatcatt gatcagataa gagcaaaaga
    13921 agtaccggtt ttattcgtgg aaaccagtgt agactcaaga agcatggaac gggtagcaaa
    13981 agaaacgggt ttgaaaatct atgataaact gttcactgat tccatagcaa aagaaggaga
    14041 acaaggagat tcttactatc agatgatgaa atggaatatt gaaacgatcc atgaaggttt
    14101 gtcacaaaca aaagaaagct gaaaagtgca aggtctagct tgaggagcag accttgcatt
    14161 ttttataaga gatatgaaaa aaaattatgc tgtttttcgt cgtacagata ccttttttga
    14221 agaaattccg ttatagttag agtaaggaag gaaggtgtgg ctatgaattt tacacgtaaa
    14281 gataaatata cgattaaaga agaagtcttt cgattgatca gccagcagga aaataccgac
    14341 aaggatcaag accttcaatt tcttgaagca gaattagcta aattattcat agcaaatcaa
    14401 aatgagacaa cagaaccaga tgaagagctg tacataggca aatggttgtt tgatgaattt
    14461 cagcctttag atgaaaatac cggtatcggt tattttgtgt tcctttctcc agtcggcttc
    14521 acacctggcg tattagttaa acaaaacgac gaaataaccg tcaaagcatg gcctgcatta
    14581 ttttcagtca tcaaagcaga aggagtaatc aaaaagatcc agtcggccta ttataaaagt
    14641 caatttattg ccaacagcaa attaatgaat atgttttata atcgtctagt agaaattgct
    14701 aggcctggga aaatcccgca aacattcacg tacacacagc aaaaaaatct ctatgaagaa
    14761 gtattgtatt cccgcgatct gtctgagttt tcgccatggg aagaggtaag gctccatgaa
    14821 ctgttaagta caaaaccagt ttcattgaat ttttttaatg cattagataa caaaagaaaa
    14881 ttaatttcaa caaattattg ggaagtattt gctgcaagaa aaaaatggat ggaagaaaag
    14941 aagattgccg atcagcagat ccagtttgtc cagctgaaaa aagaaaaact atattatggg
    15001 ttatacagcc gttaacagca agcgaggaag accatgacat taaaagaaga aatcatacaa
    15061 gaaagcaaac gattaggaat tgacaaaatc gggttcacca cggcggaacc ttttgattca
    15121 ttaaaagaat cgttggaaga acaaaaagca gctggccata cttctggctt tgaacatcca
    15181 aatatcgatg agcgtcttta tccagaaatg acatttgatc agccgaaatc gatcatttcc
    15241 attgcacttg cgtatcctac taggatccat gaagaagtgc ctagggatga aaaacgcggg
    15301 cagtttgctc gtgcttcttg ggggatcgat taccatgata tactcagaga ccgtttgaac
    15361 cgattgatcg aattcatcca aacaagagca gaaaaatggc aaaaagaaga ggaatggcgt
    15421 ctagctccgc aagtagatac tggagaactg atcgatgtcg ctgtagcaca gcgagctggt
    15481 ttaggattta ttggacgaaa cggattgttg atcactgaag agttcggttc atttgtctat
    15541 cttggtgaga tcgttactaa tattgtattt gagcctgata aaccaggaga atttggttgc
    15601 ggtgactgta tgcgctgcgt gactgcttgt ccgacaaaag cattgcttgg tgacggaaga
    15661 atgaatgccc agcgatgttt gtcttatcaa actcagacaa aagggatgat gccggaagaa
    15721 tatcgtaaaa aaatgcgaaa tgtcatttat ggatgtgata tctgccagtt agtctgccct
    15781 tacaatcaag gaaaagattt tcattttcat gaagagatgg aaccaaaagt agacgaagta
    15841 tatccaaaat taaagccaat gctttcgatg tctaataaag acttcaaaca gcaatttggg
    15901 catttagctg gttcatggcg tgggaagaaa cctttgcaga gaaacgcatt gattgcttta
    15961 gctaatctag gtgatcgaac ggcattgcct gagatttcag aatgtttaac agatgtccgc
    16021 cctgtcatca gaggaacagc agcttgggct attggacgtc taggaacaaa agaaccggaa
    16081 aaatggcttg atgtattaga tgaaatatta gctaaagaaa cagaagaagt cgttataaac
    16141 gaattggaac atgcccagaa attattgcgg agaaagttaa aataaaaaaa tagttaa
//
LOCUS       DADJYG010000057        16191 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.057, whole genome shotgun sequence.
ACCESSION   DADJYG010000057 DADJYG010000000
VERSION     DADJYG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.057"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(125..814)
                     /locus_tag="JZ382_001427"
     CDS             complement(125..814)
                     /locus_tag="JZ382_001427"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY2427621.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="JZ382_001428"
     CDS             complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="JZ382_001428"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY2427622.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1534..2466)
                     /locus_tag="JZ382_001429"
     CDS             complement(1534..2466)
                     /locus_tag="JZ382_001429"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAY2427623.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2463..3239)
                     /locus_tag="JZ382_001430"
     CDS             complement(2463..3239)
                     /locus_tag="JZ382_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAY2427624.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3293..6478)
                     /gene="carB"
                     /locus_tag="JZ382_001431"
     CDS             complement(3293..6478)
                     /gene="carB"
                     /locus_tag="JZ382_001431"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY2427625.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6483..7559)
                     /gene="carA"
                     /locus_tag="JZ382_001432"
     CDS             complement(6483..7559)
                     /gene="carA"
                     /locus_tag="JZ382_001432"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY2427626.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7564..8844)
                     /locus_tag="JZ382_001433"
     CDS             complement(7564..8844)
                     /locus_tag="JZ382_001433"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY2427627.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8863..9789)
                     /locus_tag="JZ382_001434"
     CDS             complement(8863..9789)
                     /locus_tag="JZ382_001434"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAY2427628.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9802..11085)
                     /locus_tag="JZ382_001435"
     CDS             complement(9802..11085)
                     /locus_tag="JZ382_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY2427629.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="JZ382_001436"
     CDS             complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="JZ382_001436"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAY2427630.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11873..12784)
                     /locus_tag="JZ382_001437"
     CDS             complement(11873..12784)
                     /locus_tag="JZ382_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAY2427631.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12781..13245)
                     /locus_tag="JZ382_001438"
     CDS             complement(12781..13245)
                     /locus_tag="JZ382_001438"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAY2427632.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13250..13948)
                     /locus_tag="JZ382_001439"
     CDS             complement(13250..13948)
                     /locus_tag="JZ382_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAY2427633.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13935..14321)
                     /locus_tag="JZ382_001440"
     CDS             complement(13935..14321)
                     /locus_tag="JZ382_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427634.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14468..16138)
                     /locus_tag="JZ382_001441"
     CDS             complement(14468..16138)
                     /locus_tag="JZ382_001441"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAY2427635.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4360 a         3732 c         2886 g         5213 t
ORIGIN      
        1 ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa aaactgatca
       61 cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag tttttggatt
      121 tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc ttcatcaagc
      181 gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga gttaattctt
      241 gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa ttggtcaacg
      301 tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac tcatacgtaa
      361 agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca gctgcttcca
      421 agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga ccttcaaata
      481 tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga atatagacca
      541 ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct gttgcagttc
      601 cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat ccttttgcga
      661 tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa attgggcttt
      721 tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct tcaatttcta
      781 ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat tctgtttgaa
      841 tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct ataacgataa
      901 aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga tctcctgcga
      961 ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct gccatttctt
     1021 tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct tttttggcac
     1081 agctagcata atggatcaca cttttttcta aagaaacttc gattagttga tcggcatgca
     1141 tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga cgttttccac
     1201 cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga ataccgccag
     1261 cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt gctttttcga
     1321 ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca gcctctttta
     1381 gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt ttcacaaaca
     1441 atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta gttgaaaaat
     1501 ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct ctctcacttc
     1561 tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag ggagctcttt
     1621 aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag ctacagcact
     1681 tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc ccatcccaat
     1741 gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga caggcttgat
     1801 tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg tcttaaggtc
     1861 aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc cagcttcgat
     1921 tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat agacaggaac
     1981 agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat ctgttccaaa
     2041 agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg ctttcacatt
     2101 tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag caccagcaac
     2161 atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt tatgcataac
     2221 aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg gtgtttccgc
     2281 cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga ccatgatcga
     2341 tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga aacagccgct
     2401 tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta aaggattctc
     2461 attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac tttcacactt
     2521 tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc ccctatccca
     2581 catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa cagctgttcc
     2641 actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc cgtcttcgtt
     2701 cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc agtagcgatt
     2761 cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg tgtggcaaaa
     2821 cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa ctcatataga
     2881 ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg aagattggac
     2941 aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc agaaagagta
     3001 gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt atttgtttcg
     3061 tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg cggcacgaga
     3121 atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt caatgtcatt
     3181 tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc ttgtttcatc
     3241 tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc cgttaaatag
     3301 ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct gtgtctaaag
     3361 aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa ccgtctttag
     3421 aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta ccatctctta
     3481 ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact gtttcaacga
     3541 ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga ctatagccaa
     3601 tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa
     3661 ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag
     3721 ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt tcaggcccta
     3781 agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta acatgaactt
     3841 cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa
     3901 tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag aatggaacag
     3961 tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg atgacaaact
     4021 ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg taatcttcga
     4081 tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt gaatcccctg
     4141 aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact gtttcaccgt
     4201 cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact agtactggat
     4261 gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc tggttttcaa
     4321 cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact ggataaccaa
     4381 ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct aaaggttgag
     4441 gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca cgatccaagt
     4501 cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg gctaagttga
     4561 ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc tcgatcacat
     4621 tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata gaaaagtcag
     4681 tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc tgctggattg
     4741 ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata cggataggac
     4801 cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt tcatattcat
     4861 aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg tcaaccatct
     4921 tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct tcgtttattt
     4981 cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct
     5041 tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc aacagtttat
     5101 ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg taccctcttc
     5161 taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact ttttggatta
     5221 ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta ccgatttcta
     5281 aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc atcacttcac
     5341 cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc
     5401 agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca aaagtcgttc
     5461 ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta gcagctagct
     5521 tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa acacgcggat
     5581 tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg acattacagc
     5641 ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc atttgatatt
     5701 catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta tggataccca
     5761 ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa tctctcatca
     5821 cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat tgggtagcgg
     5881 gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt tcacacatac
     5941 ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag ccgattttat
     6001 ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg attggctgat
     6061 tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa
     6121 gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct gccagctcca
     6181 ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct ggacgctctt
     6241 ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact tgatctgcca
     6301 cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact tcatatcctt
     6361 cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa
     6421 tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt ttcggcatct
     6481 gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa atgtagtgca
     6541 tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg ataatctctg
     6601 tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat gagtttttct
     6661 ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa atcaattctt
     6721 ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt cattttatag
     6781 gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc aaaaatcggt
     6841 acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt tggatcacca
     6901 ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg
     6961 ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa aatactatgt
     7021 ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg ataaggtttg
     7081 ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag ctgatcaaat
     7141 gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc gacttcacgg
     7201 attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc tttttgtttc
     7261 aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc atgttctttt
     7321 actactactc ctttgcatgt tggagaaatc gattcataat catctctatt taccccataa
     7381 ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg atcagtgata
     7441 gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac actagcatct
     7501 gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa acgttccata
     7561 cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt gtgtttcgcc
     7621 ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt cttctttatc
     7681 gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac caaccgttag
     7741 cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat tgatcacttg
     7801 ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa acgctgtttc
     7861 gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt ctaatccatg
     7921 gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc
     7981 ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga aatcttcggg
     8041 aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta cgtggattcc
     8101 tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat ggcaaacatg
     8161 gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag aagactcggt
     8221 tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt gcataacgcc
     8281 gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt tttttgcagc
     8341 ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt catttgtgaa
     8401 agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac ttcgcagatt
     8461 ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa tgatgtcata
     8521 tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca tcgcgcatac
     8581 agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct ccttgtatgt
     8641 aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga tcaattgtcc
     8701 ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga ttgctttgat
     8761 tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg tattgatctg
     8821 accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt ttccatttag
     8881 cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct gaacgatccg
     8941 cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa ctggtgctgg
     9001 gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta acccatgttc
     9061 ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt gttgcacccg
     9121 aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat aatgtccgta
     9181 cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt gtgcgcctag
     9241 acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat cacctacgat
     9301 ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca aatcaagcaa
     9361 gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac actggattgt
     9421 tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa ccgctacgtc
     9481 tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt tttggacaga
     9541 gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg ctacatcgaa
     9601 gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt actggcgctt
     9661 ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca agcccataac
     9721 ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac gttctgattt
     9781 gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg gtaagataag
     9841 atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa atgtgcctac
     9901 ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca acagattttt
     9961 ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg cggcaattac
    10021 tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta aaatcaaagc
    10081 gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac cgatcacaaa
    10141 cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac ttgttacagg
    10201 aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca acgtcttatg
    10261 aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca taagatggcc
    10321 gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg ctccaagata
    10381 tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa accaggcagc
    10441 gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt atccgctaat
    10501 aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt tgaagaaaat
    10561 tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg tgccctgatt
    10621 atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca tgatgactgg
    10681 accgacgacg attggaggca agattttatc cagccatgct gaaccgatct ttttgatgat
    10741 cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa tcgctggata
    10801 accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta
    10861 cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc ctgaactgac
    10921 tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg ctccgaacat
    10981 cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc gatcgtggat
    11041 atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc tctctcctct
    11101 aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc atctctacta
    11161 aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca cggattggca
    11221 attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt ctgccaaaat
    11281 ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca
    11341 aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt tccggttctt
    11401 ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct acaggaatat
    11461 cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag attcctcgtg
    11521 ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata atctcataag
    11581 taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt gcttgcattt
    11641 tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag gaggaatgtt
    11701 tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca atctacttat
    11761 ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta aacttaaagg
    11821 aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta aattaaaatg
    11881 caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa
    11941 agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca tggagaaact
    12001 gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct aacggataac
    12061 cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt tttaattcaa
    12121 ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc gacttcccat
    12181 cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc gcattgatgg
    12241 ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc aaagaggtct
    12301 tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca accattagca
    12361 gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg ccgttgatcg
    12421 tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc gtatgacctg
    12481 ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct tcatcttcgt
    12541 aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt tcggggatca
    12601 agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc tgtccattga
    12661 cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa tcggtgaata
    12721 aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt ttaagttctg
    12781 tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac aaatcactcc
    12841 acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa
    12901 catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg caccagctag
    12961 aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt taatcatcaa
    13021 tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca tcttcccttc
    13081 tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa tacctggtat
    13141 aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga ccaactgatc
    13201 cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat caatcctttt
    13261 ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca tcgggattcc
    13321 catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc gcttcaacct
    13381 cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg ttttggatga
    13441 tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt tcttttgcta
    13501 gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta ttgtataacg
    13561 gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac ggaagatctt
    13621 gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt
    13681 cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca atgacaaaat
    13741 cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt aaaagatcat
    13801 cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca agttcggtaa
    13861 atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt cgattgaaac
    13921 tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata tgcccggttt
    13981 tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa aaataaattt
    14041 tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat ttgaagaatc
    14101 aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata aacggggctt
    14161 tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag cgttatcttt
    14221 tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat cgagctaccc
    14281 ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc ctattatgct
    14341 ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt
    14401 ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg gtagattata
    14461 atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata ttcaatgcag
    14521 caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct actaaaaatc
    14581 ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta
    14641 gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc ggcagtttgt
    14701 cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg aaaacaacac
    14761 catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc gtttcttcta
    14821 ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact acagcttttg
    14881 ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg gcaagaatcc
    14941 cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct gttacgaatt
    15001 cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata tggcgcttta
    15061 aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca tcttttttca
    15121 ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca tccggcgctt
    15181 ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct gcaccaaatc
    15241 ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct aaaatactat
    15301 tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa
    15361 ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga gcgccttctg
    15421 ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca acgatccttg
    15481 caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc atgatttcgc
    15541 tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc ccacccaagc
    15601 caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc caaatcactc
    15661 ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa agagcagata
    15721 atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag ttgatatctt
    15781 ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt cccatcacag
    15841 gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta ttgagcgcgt
    15901 ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga ttgatggaag
    15961 taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca gaaaaattga
    16021 ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct aatttactag
    16081 ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca ttcttcattt
    16141 gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag a
//
LOCUS       DADJYG010000058        16178 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.058, whole genome shotgun sequence.
ACCESSION   DADJYG010000058 DADJYG010000000
VERSION     DADJYG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.058"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="JZ382_001442"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="JZ382_001442"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY2427636.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="JZ382_001443"
     CDS             complement(1061..1981)
                     /locus_tag="JZ382_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2427637.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="JZ382_001444"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="JZ382_001444"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY2427638.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3710..4243
                     /locus_tag="JZ382_001445"
     CDS             3710..4243
                     /locus_tag="JZ382_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAY2427639.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4733..5257)
                     /locus_tag="JZ382_001446"
     CDS             complement(4733..5257)
                     /locus_tag="JZ382_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427640.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5250..5723)
                     /gene="tsaE"
                     /locus_tag="JZ382_001447"
     CDS             complement(5250..5723)
                     /gene="tsaE"
                     /locus_tag="JZ382_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY2427641.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5846..6826)
                     /gene="pta"
                     /locus_tag="JZ382_001448"
     CDS             complement(5846..6826)
                     /gene="pta"
                     /locus_tag="JZ382_001448"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY2427642.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6979..7659)
                     /locus_tag="JZ382_001449"
     CDS             complement(6979..7659)
                     /locus_tag="JZ382_001449"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY2427643.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7785..8654
                     /locus_tag="JZ382_001450"
     CDS             7785..8654
                     /locus_tag="JZ382_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427644.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8680..9195
                     /locus_tag="JZ382_001451"
     CDS             8680..9195
                     /locus_tag="JZ382_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427645.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9242..9805)
                     /locus_tag="JZ382_001452"
     CDS             complement(9242..9805)
                     /locus_tag="JZ382_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427646.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9860..10039)
                     /locus_tag="JZ382_001453"
     CDS             complement(9860..10039)
                     /locus_tag="JZ382_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427647.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10116..10625)
                     /locus_tag="JZ382_001454"
     CDS             complement(10116..10625)
                     /locus_tag="JZ382_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427648.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10721..11101)
                     /locus_tag="JZ382_001455"
     CDS             complement(10721..11101)
                     /locus_tag="JZ382_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY2427649.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11431..12180
                     /locus_tag="JZ382_001456"
     CDS             11431..12180
                     /locus_tag="JZ382_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAY2427650.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12364..13641)
                     /locus_tag="JZ382_001457"
     CDS             complement(12364..13641)
                     /locus_tag="JZ382_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427651.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13720..14364)
                     /gene="pcp"
                     /locus_tag="JZ382_001458"
     CDS             complement(13720..14364)
                     /gene="pcp"
                     /locus_tag="JZ382_001458"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAY2427652.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14563..15507)
                     /locus_tag="JZ382_001459"
     CDS             complement(14563..15507)
                     /locus_tag="JZ382_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAY2427653.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15509..>16178)
                     /locus_tag="JZ382_001460"
     CDS             complement(15509..>16178)
                     /locus_tag="JZ382_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAY2427654.1"
                     /translation="LLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLG
                     TSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFA
                     AFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVL
                     FIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYP
                     GKGQ"
BASE COUNT         4647 a         3361 c         2870 g         5300 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttggactta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat cagcgtcctg
     3361 acctgagtat tagagaaaca aatggcaaaa tagttcctca tattttgcta ttttttgagc
     3421 taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa aaagtcgcct
     3481 actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc ataaatttga
     3541 cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat gagttcaaaa
     3601 actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta aatttatttg
     3661 cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa tgaattttgt
     3721 tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc ttgcgctagt
     3781 catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc ctgagactcc
     3841 ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac gcaatgcgcc
     3901 gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc gtttagtcgt
     3961 tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt attatcatct
     4021 tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc tgtttcctga
     4081 atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat tagatcatca
     4141 ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc aagaaaaaaa
     4201 attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata cttatgaaac
     4261 acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa tttcaaaaaa
     4321 tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca ggagaacacc
     4381 gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
     4441 aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca agcttttgga
     4501 caccgtttac caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
     4561 gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
     4621 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
     4681 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
     4741 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
     4801 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
     4861 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
     4921 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
     4981 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
     5041 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
     5101 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
     5161 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
     5221 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
     5281 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
     5341 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
     5401 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
     5461 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
     5521 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
     5581 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     5641 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     5701 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     5761 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     5821 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     5881 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     5941 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     6001 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     6061 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     6121 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     6181 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     6241 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     6301 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     6361 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     6421 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     6481 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     6541 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     6601 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     6661 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     6721 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     6781 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     6841 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     6901 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     6961 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     7021 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     7081 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     7141 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     7201 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     7261 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     7321 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     7381 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     7441 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     7501 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     7561 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     7621 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     7681 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     7741 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     7801 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     7861 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     7921 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     7981 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     8041 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     8101 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     8161 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     8221 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     8281 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     8341 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     8401 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     8461 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     8521 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     8581 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     8641 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     8701 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     8761 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     8821 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     8881 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     8941 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     9001 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     9061 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     9121 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     9181 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     9241 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     9301 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     9361 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     9421 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     9481 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     9541 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     9601 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     9661 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     9721 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     9781 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     9841 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     9901 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     9961 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
    10021 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
    10081 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
    10141 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
    10201 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
    10261 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
    10321 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
    10381 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
    10441 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
    10501 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
    10561 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
    10621 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
    10681 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
    10741 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
    10801 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
    10861 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
    10921 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
    10981 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
    11041 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
    11101 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
    11161 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
    11221 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
    11281 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
    11341 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
    11401 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
    11461 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag
    11521 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
    11581 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
    11641 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
    11701 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
    11761 tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca
    11821 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
    11881 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
    11941 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
    12001 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
    12061 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
    12121 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
    12181 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    12241 aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
    12301 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat
    12361 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
    12421 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
    12481 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
    12541 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
    12601 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
    12661 ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat
    12721 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
    12781 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
    12841 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
    12901 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
    12961 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
    13021 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
    13081 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
    13141 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
    13201 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
    13261 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
    13321 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
    13381 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
    13441 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
    13501 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
    13561 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
    13621 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
    13681 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
    13741 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
    13801 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
    13861 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
    13921 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
    13981 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
    14041 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
    14101 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
    14161 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
    14221 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
    14281 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
    14341 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
    14401 tatcttttat cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata
    14461 acataacaga tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag
    14521 aagaggttgc gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat
    14581 ataagcaatt tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc
    14641 gtatttgtct ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg
    14701 tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt
    14761 cgctccatga ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc
    14821 aaatgcattc cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc
    14881 gatgttcaca tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac
    14941 tccagcgctt gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg
    15001 ccctactgtg cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg
    15061 agaaaagaac atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc
    15121 acctaaattg gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat
    15181 aaaaattttc attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg
    15241 gctttctcct ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc
    15301 accgttcacg attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca
    15361 gaaaaaggct gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt
    15421 gtaaatcaat acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa
    15481 aatatcagga tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg
    15541 ctaatttctt ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca
    15601 ctggaacttc cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg
    15661 ttgattggac tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc
    15721 agacattttc catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt
    15781 ctaacccttt tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc
    15841 cagcaactcc accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa
    15901 tatctacaat cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt
    15961 ctttcagacc atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca
    16021 tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac
    16081 cgccagtcaa agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa
    16141 atgcaaaccc gagaataaca atcaagacgc ctaaaagg
//
LOCUS       DADJYG010000059        16098 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.059, whole genome shotgun sequence.
ACCESSION   DADJYG010000059 DADJYG010000000
VERSION     DADJYG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.059"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..855
                     /locus_tag="JZ382_001461"
     CDS             25..855
                     /locus_tag="JZ382_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY2427655.1"
                     /translation="MIGLAILLSGCSKWIDRGESITAVGSSALQPLVETVAETYQTEN
                     PGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRVAVVGITPIV
                     NKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRSTFEKWVLDGE
                     TAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTDENVTTNKWT
                     IWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVERDWQGNIVKE
                     "
     gene            970..1890
                     /gene="pstC"
                     /locus_tag="JZ382_001462"
     CDS             970..1890
                     /gene="pstC"
                     /locus_tag="JZ382_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAY2427656.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1890..2774
                     /gene="pstA"
                     /locus_tag="JZ382_001463"
     CDS             1890..2774
                     /gene="pstA"
                     /locus_tag="JZ382_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY2427657.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2789..3595
                     /gene="pstB"
                     /locus_tag="JZ382_001464"
     CDS             2789..3595
                     /gene="pstB"
                     /locus_tag="JZ382_001464"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAY2427658.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3611..4369
                     /locus_tag="JZ382_001465"
     CDS             3611..4369
                     /locus_tag="JZ382_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAY2427659.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4382..5059
                     /gene="phoU"
                     /locus_tag="JZ382_001466"
     CDS             4382..5059
                     /gene="phoU"
                     /locus_tag="JZ382_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY2427660.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5297..6328
                     /locus_tag="JZ382_001467"
     CDS             5297..6328
                     /locus_tag="JZ382_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAY2427661.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6325..7092
                     /locus_tag="JZ382_001468"
     CDS             6325..7092
                     /locus_tag="JZ382_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAY2427662.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7192..8760
                     /gene="liaX"
                     /locus_tag="JZ382_001469"
     CDS             7192..8760
                     /gene="liaX"
                     /locus_tag="JZ382_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAY2427663.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9060..9377
                     /locus_tag="JZ382_001470"
     CDS             9060..9377
                     /locus_tag="JZ382_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAY2427664.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9377..9727
                     /locus_tag="JZ382_001471"
     CDS             9377..9727
                     /locus_tag="JZ382_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAY2427665.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9894..10832
                     /locus_tag="JZ382_001472"
     CDS             9894..10832
                     /locus_tag="JZ382_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAY2427666.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10847..11677
                     /locus_tag="JZ382_001473"
     CDS             10847..11677
                     /locus_tag="JZ382_001473"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY2427667.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11721..12746
                     /locus_tag="JZ382_001474"
     CDS             11721..12746
                     /locus_tag="JZ382_001474"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY2427668.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12761..13699
                     /gene="galU"
                     /locus_tag="JZ382_001475"
     CDS             12761..13699
                     /gene="galU"
                     /locus_tag="JZ382_001475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY2427669.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13824..14264
                     /locus_tag="JZ382_001476"
     CDS             13824..14264
                     /locus_tag="JZ382_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAY2427670.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14267..14869
                     /locus_tag="JZ382_001477"
     CDS             14267..14869
                     /locus_tag="JZ382_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAY2427671.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14928..16034)
                     /locus_tag="JZ382_001478"
     CDS             complement(14928..16034)
                     /locus_tag="JZ382_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAY2427672.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5209 a         2814 c         3486 g         4589 t
ORIGIN      
        1 aaaaaactta aattattctt accaataata ggattagcta ttcttttatc aggctgttca
       61 aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt acagccttta
      121 gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat caatgtacaa
      181 gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga catcggaaac
      241 tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt cgatcatcga
      301 gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa agacatcagt
      361 atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga agtcggtgga
      421 aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg tagcacgttt
      481 gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga ttcaagcgga
      541 atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc tttttcttat
      601 gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg
      661 acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga tcatccaaaa
      721 aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga taacatcgtt
      781 ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat
      841 atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt tacatgttat
      901 taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct gattaagggg
      961 gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg
     1021 gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta
     1081 tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga taagatcaat
     1141 gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc aaatgggaaa
     1201 ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt tttatctgct
     1261 atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat ctctcctaaa
     1321 aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc ttctgtcgtt
     1381 tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt tggcgggact
     1441 gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca
     1501 acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc ttcattagcg
     1561 cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg
     1621 attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt agctatccaa
     1681 atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc ttctacattg
     1741 acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga aaataatgtg
     1801 ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat cgtgatccgt
     1861 atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat aaaattgcta
     1921 caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct ttactacttt
     1981 atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa ccttccaaag
     2041 cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat ttgcttttga
     2101 tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt tcagaatatg
     2161 ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg agctcgttgc
     2221 cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc cagtatggtt
     2281 tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt ttgactagaa
     2341 cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga ttggccttag
     2401 gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca ccagggattt
     2461 tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca ttgatctata
     2521 cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta agtgtttcaa
     2581 gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg aagatcaata
     2641 cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc gtgttgatta
     2701 ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa
     2761 tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt
     2821 catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac atgtctggta
     2881 tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata agatcactgc
     2941 actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa
     3001 tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg tagatattaa
     3061 tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt ttcaacgtcc
     3121 taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac agcacggcga
     3181 aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg
     3241 ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg ggcaacagca
     3301 acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc tagacgagcc
     3361 ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg taaacttgaa
     3421 agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc gtatcagcga
     3481 ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta acaagatatt
     3541 taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg
     3601 aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt attatggaaa
     3661 gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca ctgcgatgat
     3721 cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga tgaatgacct
     3781 gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca tctatggtcc
     3841 aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc agccaaaccc
     3901 atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag gggaaaaaga
     3961 taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg tttgggatga
     4021 tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt
     4081 atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg aaccaacaag
     4141 tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat taagagagcg
     4201 gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt cagataagac
     4261 ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga tattcatgaa
     4321 tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag gaggaaacgg
     4381 aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt tctacgaaat
     4441 ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca aacacgataa
     4501 aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg aaacacgctt
     4561 ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg atttgcgtat
     4621 gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc atgcggtgtc
     4681 tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga
     4741 gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt
     4801 caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag tcaatgacta
     4861 tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg agacagtcat
     4921 cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag actatgtgac
     4981 aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt taaatacgaa
     5041 ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg aaaagaggtt
     5101 gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa
     5161 aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca agcttttgaa
     5221 caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt tcatcatttc
     5281 atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt ttatttgtgt
     5341 gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg tcatcgagat
     5401 agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga tgaacacgga
     5461 tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa tagaaacagc
     5521 aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat ggcagctagt
     5581 agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg gcgccatgtt
     5641 gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca atatcgtttt
     5701 acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat taaatatatc
     5761 tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg gatggaatag
     5821 aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat tttttatttg
     5881 tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac tgaattatct
     5941 gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag ctggaggaac
     6001 actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc gacaaccaga
     6061 aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg tgattggctc
     6121 gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc ttcttttata
     6181 tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat tggaattttt
     6241 acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg tcctcttttt
     6301 tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg gattttagca
     6361 atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg cttattcatc
     6421 aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc gatactggca
     6481 ttgtcggtca taggattttt attgattgct gtggttatct tattttttat caacggttat
     6541 aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg tttcctacta
     6601 ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga actagaagaa
     6661 ttatctggct tagataagac tttagcgatt gattttagat tacttacgag atcgttgctt
     6721 ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag tgggacattc
     6781 gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc tagtttcgct
     6841 attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt catgggaagt
     6901 tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca agtgatcaat
     6961 gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc cgcactactt
     7021 gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa agaacaactc
     7081 aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc agtgcataat
     7141 atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc catgaaagaa
     7201 agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga aggcttagat
     7261 ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga agcagacaag
     7321 gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa acttgaaaac
     7381 ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca agatcaagaa
     7441 aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc ggcgcgtcta
     7501 gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa acaggaagca
     7561 ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga attagcacag
     7621 cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac actgttagaa
     7681 gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg gtctgagaaa
     7741 aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca agcaacagat
     7801 acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg caaattcttg
     7861 aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg gaaagatgtc
     7921 agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta ctatgaagct
     7981 cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt aaaaacgtgg
     8041 gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat gggtgctgaa
     8101 ccatttgaag cattctctga acggagccag attgaggtta atgaagatca tatctccttc
     8161 caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa acgagtatac
     8221 gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt ggaagctaaa
     8281 gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac tgcaacgatg
     8341 ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat cttagattct
     8401 attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa cctttctgtg
     8461 tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa aaaagtagaa
     8521 gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg tttggaagga
     8581 catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga agtagttcgt
     8641 gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga tggtgaaatc
     8701 gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac agataaataa
     8761 agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt aaaatagctt
     8821 cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac accgtttacc
     8881 aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca gaaaaagctc
     8941 gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga caccgattaa
     9001 gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg tgagacatga
     9061 tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact ttagcaggaa
     9121 taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta ttcctgagct
     9181 tagttgcttt tggaagtcca attattttat acattttatt agctttgatt atcccatctg
     9241 cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa aacaattatc
     9301 gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg
     9361 aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt taacattcat
     9421 atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga cggccgttct
     9481 tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga cgatactgtc
     9541 cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg cagcgattct
     9601 taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat tcggtgcagc
     9661 gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac ataacagtag
     9721 aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga atcaggaatg
     9781 cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta gtacttttgg
     9841 ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca tgaatgtcag
     9901 aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg
     9961 agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga ctagagttga
    10021 ctggttattt taattattat tcccataatc gtttacagct atttggcagt aaagaaatca
    10081 gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga ttatgtagtg
    10141 aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa atgatccagg
    10201 cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct cgtttattag
    10261 gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt
    10321 tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg
    10381 aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat cgagtagatg
    10441 tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg gaacatctga
    10501 ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg agtgcggtac
    10561 gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa gataagaaat
    10621 acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc attccacaaa
    10681 taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta gccgcgatga
    10741 atttccgcgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa agattaagtc
    10801 gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt tagcacaaat
    10861 caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat tgatcatcgt
    10921 atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg taggattaaa
    10981 agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta ttggcgctcg
    11041 cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc aaatattcaa
    11101 tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga tcacgctgta
    11161 tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg ctgcaccaag
    11221 tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg aagcatatgg
    11281 accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca tcattgataa
    11341 tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac tttggagttt
    11401 gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga aaaaaggcga
    11461 agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg aaggattacg
    11521 tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat ctgctgtgtt
    11581 atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt taccggatta
    11641 tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa
    11701 aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca ggctcttggg
    11761 gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt tggggacatc
    11821 gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat ttaccagatc
    11881 attatctacc cgattccatc caagcattca ccaatatgga agatgcagta aaagacgcag
    11941 atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc
    12001 ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa cagggaacac
    12061 ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga aaagctgttg
    12121 ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc actacgatca
    12181 cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc atgaataatt
    12241 attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca gctttgaaaa
    12301 atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat aatgcaaagg
    12361 cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca atgggtgcca
    12421 acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact tgcacaagtg
    12481 ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc ctagaagagg
    12541 tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca gcgatggaac
    12601 tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa gtgctgtatg
    12661 aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga aaaaaagaaa
    12721 atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta aaaaagcagt
    12781 tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta tggcaaagga
    12841 aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag cgttgaaatc
    12901 aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg aagatcactt
    12961 tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa
    13021 attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc atccaaaggg
    13081 tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat ttgtcgttat
    13141 gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa tcgatgatta
    13201 cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg aaacttcaaa
    13261 atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg tcaaaaactt
    13321 tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg gacgttactt
    13381 attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg gaaatgaaat
    13441 ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg cccgtaaatt
    13501 cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa gtattgaata
    13561 tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt ctttaggtaa
    13621 aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa cagaaatcaa
    13681 agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat tgaataaaaa
    13741 gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct tatagaaaag
    13801 agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg atcgcagcaa
    13861 tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt tctaaaactt
    13921 tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc gtcacaaaag
    13981 acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat gaactgctct
    14041 tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt gctgatttaa
    14101 gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa gtcggcggaa
    14161 tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca gcgatgaaat
    14221 tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg ctaaaaaagg
    14281 tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg cgttattgtt
    14341 agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg atgatttgat
    14401 ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt taacaggtat
    14461 tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag gtagtgtcaa
    14521 acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata tcttgttaaa
    14581 tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag aagatatgaa
    14641 aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata tcatgattga
    14701 tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt cagaagcaaa
    14761 accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg cgaaagaagc
    14821 taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa atcacaaatt
    14881 tatagaataa aaaggtggga gcggactagc tctcaccttt tttactctta tttttctaaa
    14941 acaagtaatt cttttggtgt agtcgtgaat ggcacacagc catcgcttgt cacatataca
    15001 cagtcttcga tacgtacccc tactttttca ggaatataga ttcctggttc aagagagaag
    15061 cacatccctt cttcgattac taagtcattt ccttctacaa gggaagggaa ttcgtggacg
    15121 gttgttccga tcccgtgacc taaacggtga ttgaaatatt cgccgtagcc atagctgttg
    15181 atcacgtttc gtgcgatttg atcaagttca cctgctgtca cacctggacg gacggcttct
    15241 tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt tttcttggaa gtcggttggt
    15301 ttttgataag caattgtacg agtagcatca ctgcaatagc cattccaaac aacgccaaga
    15361 tcgaataaca caagttcatt cggtgaaatc ttcgtatttc ctggtgtacc atgagggctt
    15421 gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac accttgtttt
    15481 ttcaattggt attcaatttc tgcaatgatc gcttgttcag tcacgccttc tttcactgct
    15541 ttaaagccaa tctcaaatgc aacatcggcc catttaccag cttcgatcag tttgtctttt
    15601 tcctctagag ttttgatcag ttgtagacgt tggatgatgg aagttagatc agtgtaagaa
    15661 tccgttcccg tcaagatatc agagatagca tcgaagcgct cgacagtcaa ggcgttcttt
    15721 tcaatcccta cattgccagg ctgaccatag cgagcacgaa gttcttttcc gatgatctcc
    15781 caagggtttt cgctgtctaa atagccacgg acatcatacg cccaactact gttttgagca
    15841 tcttcaactt ccaaagcagg agtgaaaagg aaaggatctt gttcaagagg gataaaaaga
    15901 gctagtacac gctcatgagg atcactttcg tatccggaaa agtaagcgat gtggccaggg
    15961 tcgctgatat aagccaattc gacttggtgt tgtgccatcc attgacgtag ttcattgatt
    16021 tttacgttat tcatttggca acctactttc taaattaatt ttgtattcaa taattgtacc
    16081 actgatttat taaaagac
//
LOCUS       DADJYG010000060        15951 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.060, whole genome shotgun sequence.
ACCESSION   DADJYG010000060 DADJYG010000000
VERSION     DADJYG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.060"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(390..812)
                     /locus_tag="JZ382_001479"
     CDS             complement(390..812)
                     /locus_tag="JZ382_001479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY2427673.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="JZ382_001480"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="JZ382_001480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY2427674.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="JZ382_001481"
     CDS             complement(2255..3157)
                     /locus_tag="JZ382_001481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY2427675.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /locus_tag="JZ382_001482"
     CDS             complement(3171..4727)
                     /locus_tag="JZ382_001482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY2427676.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="JZ382_001483"
     CDS             complement(4750..5292)
                     /locus_tag="JZ382_001483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY2427677.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="JZ382_001484"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="JZ382_001484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY2427678.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="JZ382_001485"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="JZ382_001485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAY2427679.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="JZ382_001486"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="JZ382_001486"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY2427680.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="JZ382_001487"
                     /pseudo
     CDS             complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="JZ382_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10029..10775)
                     /locus_tag="JZ382_001488"
     CDS             complement(10029..10775)
                     /locus_tag="JZ382_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY2427681.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="JZ382_001489"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="JZ382_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY2427682.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="JZ382_001490"
     CDS             complement(11241..11519)
                     /locus_tag="JZ382_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY2427683.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="JZ382_001491"
     CDS             complement(11632..12600)
                     /locus_tag="JZ382_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2427684.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="JZ382_001492"
     CDS             12883..13857
                     /locus_tag="JZ382_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAY2427685.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="JZ382_001493"
     CDS             13862..14437
                     /locus_tag="JZ382_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY2427686.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="JZ382_001494"
     CDS             complement(14504..15643)
                     /locus_tag="JZ382_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAY2427687.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4358 a         3443 c         2856 g         5294 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga t
//
LOCUS       DADJYG010000061        15453 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.061, whole genome shotgun sequence.
ACCESSION   DADJYG010000061 DADJYG010000000
VERSION     DADJYG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.061"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(209..575)
                     /gene="ssrA"
                     /locus_tag="JZ382_001495"
     tmRNA           complement(209..575)
                     /gene="ssrA"
                     /locus_tag="JZ382_001495"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(746..1519)
                     /locus_tag="JZ382_001496"
     CDS             complement(746..1519)
                     /locus_tag="JZ382_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427688.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1663..2013
                     /locus_tag="JZ382_001497"
     CDS             1663..2013
                     /locus_tag="JZ382_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAY2427689.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1994..2710
                     /locus_tag="JZ382_001498"
     CDS             1994..2710
                     /locus_tag="JZ382_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2427690.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2710..4158
                     /locus_tag="JZ382_001499"
     CDS             2710..4158
                     /locus_tag="JZ382_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2427691.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4228..4737)
                     /locus_tag="JZ382_001500"
     CDS             complement(4228..4737)
                     /locus_tag="JZ382_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427692.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4727..5452)
                     /locus_tag="JZ382_001501"
     CDS             complement(4727..5452)
                     /locus_tag="JZ382_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427693.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5675..7795)
                     /locus_tag="JZ382_001502"
     CDS             complement(5675..7795)
                     /locus_tag="JZ382_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY2427694.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8025..9203
                     /locus_tag="JZ382_001503"
     CDS             8025..9203
                     /locus_tag="JZ382_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAY2427695.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9219..9977
                     /gene="aroD"
                     /locus_tag="JZ382_001504"
     CDS             9219..9977
                     /gene="aroD"
                     /locus_tag="JZ382_001504"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY2427696.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10000..10485
                     /gene="ybaK"
                     /locus_tag="JZ382_001505"
     CDS             10000..10485
                     /gene="ybaK"
                     /locus_tag="JZ382_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAY2427697.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10556..11809)
                     /locus_tag="JZ382_001506"
     CDS             complement(10556..11809)
                     /locus_tag="JZ382_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY2427698.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11927..13276
                     /gene="gdhA"
                     /locus_tag="JZ382_001507"
     CDS             11927..13276
                     /gene="gdhA"
                     /locus_tag="JZ382_001507"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY2427699.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13388..14734
                     /locus_tag="JZ382_001508"
     CDS             13388..14734
                     /locus_tag="JZ382_001508"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY2427700.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15042..15428)
                     /locus_tag="JZ382_001509"
     CDS             complement(15042..15428)
                     /locus_tag="JZ382_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAY2427701.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5042 a         2760 c         3126 g         4525 t
ORIGIN      
        1 gagtgtacga acaaaagttc tttttgtaaa ccttaaaata gattttcctg aatacgtatt
       61 ggggatttct aaatgtttga atgttttttt gaatgcaaaa tcaatgcaaa aaattttgta
      121 tttctctact tttctctcct tgaccaaaat aaaaaaacgt tgatttaaca acgtttttca
      181 tacatgttta tctgtctcaa ccatacatat atggagacgg cgggagtcga acccgcgtcc
      241 aaacacatcg ccacttaaaa atctacgttc atagtcaact catttaagga ttcactttac
      301 agcttgccga gtgacaggca ttctgtatcg ctagtctgat gttctctttt tcaacttaca
      361 gacggaaagt tgaaacgtat cccactaaag ttaggaccct tactcgagca catgggcgat
      421 gccgagagga tctacgctaa ctgtttttag gcagctaaag cgtaagagtt gttttcgttt
      481 ttagcagtta tatttaactg taacgttttt acgtagacgt aacctacgaa acgcaattca
      541 agctcgactg tgcctgtcga atccgtaacg tccccgttag ctaacaaggg ttacgtgtat
      601 ttttcataca aaattagtat agcatatatt gggataaatt caataaaaaa gcgaatcata
      661 tcccattatt tatataataa ctgccccttt cttaagaaaa aataagcgat tttcttaaga
      721 aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat gcgtggacaa
      781 ttccgccttc tgtatttttc tttgtgataa aggtagaaag ttcttttaat ccgtcatagg
      841 cgttgcccat agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca ttcggaccat
      901 cgccaaatcc aaaagtagga actttttcca gctgaagttc tttttgcaga cgtttgacac
      961 ctgccccttt tgagattcct ttcttcactg tatcgatcga gtaaggacca ttccgataaa
     1021 aagttagctc aggaaaatgt tcgtgatagt attcgtcacc tgattgagat aagatcagca
     1081 acatatttac tgtgttttct ttaaagccta aaggatcaat ttccggaaca tctgaatgaa
     1141 tgaagtcgta agcattgatc attgtttggc tatgaccggt acaccagatt ttctgatcgt
     1201 tatagtaaga aagttcatga ccgttcaact gtacatgatc atacattttt tgacattctt
     1261 ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa gaaccattca
     1321 tggtgaccgc agaagtaatg cccgcatctt ccatgataga gtggatttct gcttctgtac
     1381 gtcctgtagc gatgacgggg agaacattgt tttcttttaa tgcgttcata gcttttgcca
     1441 cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc atcaagatca aagaaagtga
     1501 ttgcacgata atttgacaca aataaaactc cttttttatt ggaataagtt tatcttaaca
     1561 aagaatgaac aaaagacaaa cctttcgtga agaattcctg aacatattgg ttcagtcaac
     1621 aatttagagt atactataag aagagaattt aggagggaaa gaatgatttt tttcagtatt
     1681 ggtatcttat tcttaataat tggcagtctt tttcggattc tgccctcaaa aggaaatctg
     1741 cctttttatg ggtatcattc gcctcttgcc gcaaaaacag acgctcattg gcggctggct
     1801 caaaagacaa gcggaaactg gttttttctt atgggcttac tgatggcatt gatcggctat
     1861 tatttaaaga catctgggca tacgaattat tttctgattg aaatgcttct gctagtattt
     1921 ccaattatgc ctatttttat tatgacagaa aagaaattac agaaatatga cctggaaact
     1981 ggagggaacg ataatgaata ttttaatgat tgaagataat caatctgttt ctgaaatgat
     2041 gcagatgttt ttcttaaatg aaggttggga agcaactttc aagtatgatg gaaaagaagg
     2101 gctcgatgcc tttttagaaa acccacaaca ttgggatatg attacactag acctgaattt
     2161 gcccacactg gacggtatgc tagtaggaag agagatccgc aaagtttcaa gtactgtgcc
     2221 gattatcatg ctgacagcta gagattctga aagcgatcag gtgatcggct tagagatggg
     2281 ggcagatgat tatgtcacaa aacctttcag tcctctgacg cttattgctc gaatgaaagc
     2341 tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa gcagcagttt cagatgatca
     2401 ttttgatatc gagactagtc atttcaagat gaatacgaaa acacgagaag cgtatctgga
     2461 caaccggcca attgaaggac tgacgcctaa agagttcgat ctattgttta ctttagcaaa
     2521 aaaaccacgt caagtattct ctcgggagca gttgcttgaa ttagtctggg attatcagta
     2581 ctttggtgat gaacggacag tggacgccca tatcaagaaa ttacgacaaa aaatcgaaaa
     2641 ggtcggacca caagtcatcc aaactgtttg gggagtcggc tacaaattcg atgattctgg
     2701 tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt tttgcggaat ggtcgcgttg
     2761 atcttattga tcgttggagt ttcttttaca caactgacaa aacaaacgat tgaagaaaac
     2821 aattaccagc aactgtttgg atatgctgaa tcagttgaaa agacaacgca aacctatgcc
     2881 gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa atgcattgct gctgacagag
     2941 caagtactga cagaacaaaa tgtgaacttt atatttatcg ataaaaacga acgtgtcatt
     3001 tatccgacta atacagcttt actgaatttt accttaacag ctgatcaatg gaaaaatctg
     3061 aggaatggac gtcaagaaaa attcacatcg aataaaaata ttttaggaca agcacaggcg
     3121 acctcttacg cacttgtgcc atttaatttg aatcatgaat tctatggagc acttgtagtt
     3181 agtcaacctg cgcgaaatat cgataacagc gttcgtccaa ttacactgaa cctgttcaaa
     3241 ggatttattt tttccagtat cgttgcagtt attgccagct atgcttttgc agcattccaa
     3301 gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg taattttgat
     3361 gtacagctcc ccgtacatga caaagatgaa tttgatgagt tagcagatga tttcaataaa
     3421 atgacgaatt cattgaaaga atctcaagca gaaattgaag aacaagaaaa tcgtcgaaga
     3481 caatttatgg cagatgcttc tcacgagatg agaacacctc ttacgacaat caacggactt
     3541 ctggaagggt tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc gattaaattg
     3601 atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg aaaacttgga ttatgaaaaa
     3661 atccgtacga atcaaatcag tatggtcatt aaaaaattca atggaacaga aacattgaaa
     3721 aatattgttg cacagttaga agctaaagca gaagcagcag gagatacatt gacattgaaa
     3781 gccgttgatg atattgatgt ttacgctgat tatgaccgat ttgtccaaat catggtcaat
     3841 attattcaaa acgcgataca gtttactgaa aacggccaaa tcacgattac tttagaaaaa
     3901 ggctatcttg aaacgatcat cacgattgaa gatacgggaa ttggaatgtc tgaacaacaa
     3961 atgaaaagta tctgggatcg ttattataaa gtagatcctt caagaaaaaa tacaaaatat
     4021 ggtgaatcag gattaggttt accaatcgtg cagcaacttg tacgcttgca taaaggaaaa
     4081 ttagaagtag aaagcgaact aggaaaagga acaaaattcg ctgttcgttt gcctgacgta
     4141 gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc atgaatcgat cttctatacc
     4201 gcttgttttt actataaaat gattgattta gcccgcgggc tctaaacgat caatataagg
     4261 actttgagca atagtagcat catgagtaaa gagtaagata ccttgatttt cttcatgagc
     4321 taaataagaa agcaagtgga gtagctcttg gatctccaat acactaagtt catcaaatgt
     4381 atcttcaata acaatattgt ttcgcttgga aagcaaggca tgaagtaatt gcagctttac
     4441 tttttcaaaa gacgtcaact cagctgccgc acaattaagc aaggaaggag agatatcaag
     4501 tttttcttga taggccaaac gatcttgttt gatttctttt tccggtacat ccaacaggac
     4561 atggtcttta agattcagat aaggaaacat cggccaattt ttttggatga tcgcaaaatt
     4621 atccagtttt gcaacaagaa cctctttttt caagtgctcc aatattttat gctgtttttc
     4681 gagaggacac aaaataacgg tcaagtgttc agcatgaatc aaatattcag gtttcattac
     4741 gtccaggcct cctactttgc aaatgatgga taaaaaaagt gaaagggaat agactaagaa
     4801 tgagacagca tccactgagc ccaataagag tttgggcaaa tcccttaagt atatctgtga
     4861 aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg ttgatccatc ggaattgtaa
     4921 gtccgcttac cgaagtcaag atatcacgga tatctggcag ttcagtagtc tttaagacaa
     4981 gaaaattgat cctttgaaga agcgcttcat aaactgactg gaagcttacg atccacaata
     5041 aaaccaagat acaacaaaga aagagaggaa agaacagctg ccagaaaata aaaaggaata
     5101 atttggtttt agaaagacca gtcaggcgcc aattgatcaa ttcctgcttg ttttgcctga
     5161 ctgaatagta aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa ctgaatatgc
     5221 cttttatata aaatcggcta gctaattcta aagccggaag aagctctgta gatgcagaag
     5281 cgccaataaa tgaaagccga tcttggactt gattggctaa agccgattgc aaatggtaaa
     5341 gagtcaggca tgctatcaat aaaaaagcag aaaagcttga aaataaacaa tagagtattg
     5401 tctgcttttt gtaataaatt aatcgagcag tagcatagcg gattattttc atacttttca
     5461 cctctataaa aagtatagaa aatgaatatg aataaagtat gaaattcctt tagtgaaatc
     5521 acgaattaaa accagaaaat attttgtaaa aagcaagagg ttgtgaagaa gctgccctat
     5581 tctattaccg tagatgcaag cttttgaaca ctgtttattc gaaaagagga gcagcgacaa
     5641 taattttgtc gctgctcctt ttaaatttat tttgtcattc ggaagaactt gtggtcgttc
     5701 cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac agttgattgt ccgttgtgtt
     5761 cactaaagac actagttgac ttgtcaccaa gagatttttc agcttctttt aatttctcta
     5821 agccattctt gtagtcataa tcactggtat caattgggtc taatccacta ttcgtataga
     5881 accgaagaag atcaccatta ttgatttggt cagaaacagc taactgttta tcaacttttt
     5941 ctttcagtga atcaatttca ttctgtactt cctcagtagg ttcagtaata gcttcaccag
     6001 tttcgttact atagatcgta ccaccgtagt aagtatattt tggtgtaaca aaatcaccat
     6061 cacggaaaga aacaagctgt tcatgatctt tcgagaatag atcttgtcct aactgaatat
     6121 aattttttgt atctacacct agtaagtgaa gtaatgtagg caaggcatca acttgtccgc
     6181 cgtaagtgtg attgattccg ccctgtgttt gtccagggat atggatcata taagggacac
     6241 gctgcatttg tgcattgtcg tagtttgtcc aagtagaact tgtttttcca actaattcag
     6301 ctagattttt atttcttgaa ttcgagacac cgtaatggtc accgtaaagg acgatgactg
     6361 aattatcata caagccgctt tctttcaaat aattgaagaa ttcttgtacg gcggtatcta
     6421 aataattggc tgtcgcaaag taaccgttga tcgtttcatc atttgtattg gctaacggga
     6481 atcctgcttc gtcattggtg aattttgaat aaggataatg gttagataca gcaatgaatt
     6541 ttgaataaaa tggttgttgc agacgttcca agtattgtac ggattgttgg aaaaatggtt
     6601 tatcatgcaa cccgtattgg aacgaattgt catcattaac atcataataa gaagaatcaa
     6661 agaaataatc atatcctaaa ttcttgtatg tctcgtttcg attccagaat gtaccagcgt
     6721 tcccatggaa taccgcacta gtgtaacctt gtgtttgact taaaatatca ggtgcagctt
     6781 ggaaagtatt ttttccgcct aattgtgtga acaaggagcc ttgattcaag ccaaacaatg
     6841 aattttccat cagcgtttcc gcatcgcttg tttttcctgc ttttacttga tggaaaaagt
     6901 tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt gatgaaaggt gtaacttcat
     6961 gctggagacc attctcatct gtcaatttat aatcgatcag gaattgctgg aagctttcta
     7021 aatggatata aatcacattt tttcctttag cgagcccgaa caaatcgtca tttggtgctg
     7081 cataatgttc ttttacataa ttttctactt ctttcatatc attgggactt gcttctgccc
     7141 gcacttggtt tgtctggtaa gtttgaacac cgtcataaac catgaaagca ttcaagccaa
     7201 gatatttgac caaataatca cgagagaatg ttcgagtcag caattctgga cgatcagctt
     7261 ccgccataaa aagatttcct gagaaaatca taactgctaa tgtagagatc gcaaaagcca
     7321 ttcttgctcg gatcgggcga gggtccaact tgattttttt tgcaatcagc agaacagtca
     7381 agataataaa atcgatccaa tagatcacat catatgggcg aaacagtcga atggcacttt
     7441 ctcccagacc actcgctact ttcccagcac ctaaaatcgt gttgatcgta atgaagtccg
     7501 tgaattctcg ataataagtg acattcgaaa aaacaagcaa tgtcaaaaga aaataaatga
     7561 ccatcatcgt gatataagca actctagggc gtctgatata aagagcaatt ccaagtaata
     7621 acaatgtagt tgcaataggg ttgataaaca agataaagta ttgaatgcca ctttcgattc
     7681 cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa ccaaagtaaa acgactagta
     7741 aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt ttttatacat ttcaagataa
     7801 tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa accatgttac acagctcact
     7861 tattctcatt tattttacta gtatgacaat atagtgtcaa ttgaaaccag aagtttatgc
     7921 tgtttcatta aggttttctt taagcttcat gaaaaaatga gcagtaagac aagtcctatg
     7981 ttttttgtta tacttgggaa gagaaagcta aagcaggtga aggaatgaaa gtaaaagtaa
     8041 aaaaaagagc aagcaatcga ttgaaaaaag gatatccact gatccaaaaa gaagaccttg
     8101 ctcatcccca aaaagaaacc acgcaatggg tggaattagt tgattcgcaa aaccaattta
     8161 tcgcaaaagg atatctagga aagcagaata agggaatcgg ttgggtattg agccagcaga
     8221 acatcccatt tgatcaatct ttttttgaag aattttttgt gaagtcaaaa gaaaaaagaa
     8281 ctttcttttt ttcagatgat tctactaccg cttttcgtat attcaatgga gaaggagacg
     8341 gaatcggcgg tctgattgtt gattattatg caggttatgc tgttttttct tggtataacg
     8401 aaacgttata tgcacatcgt gaattattga ttcaagcttt tcgtgaaatc tacccggaaa
     8461 tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa tctgcctgag agccagttca
     8521 tttacggaaa agaggcaccg gaacccttga tcgtaaaaga aaacggagta aaatttgccg
     8581 tttatttaaa tgaaggatta atgactggga tctttttaga ccaaaaagaa gtacggggat
     8641 cattagcaga aggtttcgct tctgggaaaa cagttttgaa catgtttagt tataccggcg
     8701 ccttttctgt tgctgccgca atgggagggg caatcgaaac aacaagtgtc gatctagcaa
     8761 aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa tggtttggat gtcgatcagc
     8821 aaaaaatcat tgttatggac gtattcgagt atttcaaata cgccaaaaga aaacaatttt
     8881 tatatgacat gattgtattg gatccaccaa gttttgccag aaataagaaa aaagtctttc
     8941 gcgtagcaaa agattatggg gaactggtca aggatagtct ggatatttta gcagaagacg
     9001 ggatacttat tgcgtctaca aatgctgcga atgtctcgat agaacaattc caaacaatga
     9061 ttgaagagga attccatgcc aaaaatgttt cttttgaaca acttgcttta tatcgtctgc
     9121 caaaagattt ccagacgacg gatacctttt tagaaggcaa ttatctgaaa gtgtttgttt
     9181 atcaaattaa aaataaccat tagaaggagc gatgcagaat ggtttgggta aagggattga
     9241 atataggaag cggtagacca aagatttgtg cgcctcttat cggggccgtc cgagaggaag
     9301 tattacgtga agcagttctt gcacgtgaag caggggccga tttagtcgaa tggcgagtcg
     9361 atcattatcg tgaagttttt tcttttgatg aagtagcaga aacattggtt cttatccatg
     9421 agatgcttga tggactgccg ttattattta cgtttcgtac cttagctgaa ggcggagaac
     9481 aagacatatc gatccgtcag tatcgcgacc tttatgaaca tgtgatacac acaggattag
     9541 tagatatggt cgatatcgag ttgttcaaag tagaaagtct aggaaaaaga ctgatagagg
     9601 aaatcaagtc tttaaacatt cctttaatta tcagtagtca tgattttaaa gaaacaccag
     9661 cagaccctgt cttgctttac cgattgaatg ttatggaaca ttttggagcc gatatcggaa
     9721 aactggcagt aactcccaat aatgaacgag atgttttgcg gttgatggag ttgactagac
     9781 gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc catgggaaat cttggtaaaa
     9841 tttctcgtct ggctggcgga acgacgggtt ccgttatgac atttggttcc ttaagcagcg
     9901 attctacttc tgctccagga caattaccgg tcaaagcatt gaagcaaatt attcaattat
     9961 taaatgaacc gttgtagaac aagtttgagg tgatattttt tggcaaagaa aaaaatagta
    10021 aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc cctatacaga gcacgaatat
    10081 gagtgggacg aaagccacct aagtgcgtca tcggtcgctg aacagcttcc agaaagccaa
    10141 tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga cggggcctct tgttgctgtg
    10201 ataccgggag aagccgaatt gaatctaaaa aaattagcaa aagtttcagg gaataaaaaa
    10261 gtagaaatgc tccatttaaa agatttggaa gcaacgaccg gatacatacg tggtggctgc
    10321 tcgcctatag gtatgaaaaa actatttcct acttatttag atcaaattgc agaaagctat
    10381 gaacaaatca ttgtctcggc tggcagaaga gggctacaga tggagttagc acctcaagat
    10441 atctgtgcat taacgagcgg acaatttgca gatataaaac aataaatcat acgtataaaa
    10501 gaaagaagga ggccgggatg ttgtttgtcc agtctccttc tttctttttt atacattatg
    10561 ctctttgccc ggcattcatt cctgctacat gccctgtagc gaaagccgct gtgatattaa
    10621 agccgccagt ataaccattg acatccagta attctcccgc aaaatacaga ccatcaatga
    10681 ttttactctc caatgtttta ggataaattt cctttagaga aacccctccg ccagttacaa
    10741 atgatttctc aataggaaaa gttttgttga ttgatagctt aaactctttg catagttgaa
    10801 caaaatcttg aatttgtttt tgagaggctt gttgtcctgt cagatgatcc atttcaagcc
    10861 gctcaagaaa gaaaactaac aatcgctcag gcaatatacc atgccaagca ttgaccaagt
    10921 tctttttggt tgctttactt ttttctgtca atatatgaat aagctcttgt ttggtttgtg
    10981 tagggaaaca atccagagaa agtgtcactg gttctcccgt tttctctaat tctttattga
    11041 caaaacttga acaacgcagt gcagccggac cagaaatacc gaaatgggtg aaaagtaaat
    11101 ccatcgtatg ttcagtcaat acacgacctt ttttatttaa aacgcgtaaa gtgatgtctt
    11161 gtaaagaaag cccttgaagt gttttctcct gaatatacgg ttcatccgat atcaaggggg
    11221 actctgtcgc ataaagaggc gtcaccgtgt gacccactcg cttcacgatc ttgtacccat
    11281 ctccagtaga accagtagat ggataagtcc gtccaccagt tgttaagata atggcaggag
    11341 catggaactc ttcgaattcc gtgcgtatac cgtaaatttt atgttctttg tggatcaatt
    11401 tctctactcg agtagagaat agaagcgtca catctagttc tttcagacga ttgagcaatg
    11461 cttcgataat cgtttttgat ttatttgtta caggaaacat ccgtccatga tcctcttctt
    11521 ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc gaaattattc cattgcgcaa
    11581 acgtactata taaaaacttt ccatttccag gaatatgtgc aatcaaatca tcaacaggac
    11641 gattgttcgt aacattgcac cgaccaccac ctgtcatcag tagttttttt cctgcttttt
    11701 tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc tgcactgatc gctgccatca
    11761 tcccgcttgt tccagcgccg actacgatca catcatacag tttttccatt ccctcacctc
    11821 aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa aaatgttttg agccaaaaga
    11881 gaaaatgagc taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac
    11941 agaatatgtt caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt
    12001 tttacaagca atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga
    12061 atatatcgaa aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg
    12121 agtgccttgg caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata
    12181 caactcagct atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag
    12241 tatcttgaaa tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat
    12301 cggtggagga aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat
    12361 gcgtttttgt caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt
    12421 tcctgcggga gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa
    12481 acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag
    12541 taagatacgt acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa
    12601 cgaagaaaaa gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc
    12661 catttatgca atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc
    12721 tagcgggtac atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga
    12781 aatcaataga caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc
    12841 tggagaatct gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca
    12901 aaatgaaata gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc
    12961 agaaggcgct aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt
    13021 gtggtattgt ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat
    13081 gagtcagaac gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca
    13141 aatcatggaa acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa
    13201 aaatctgctt cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc
    13261 agaaggttta gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt
    13321 gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg
    13381 agaacaaatg gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca
    13441 agagttaggg tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac
    13501 tggcgcagga agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga
    13561 atttgcacgt atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt
    13621 tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac
    13681 attcttcaat ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa
    13741 ttcaatcagc tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc
    13801 agtaaacgtt atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt
    13861 caaagagctt cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac
    13921 aacagataaa gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt
    13981 cgtgattcct gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc
    14041 aatcgctgtt agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa
    14101 agcatactca agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa
    14161 cattctttac cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca
    14221 atacttctca gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg
    14281 catctatcct tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca
    14341 agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat
    14401 cacaattcct gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt
    14461 ggacttcgta aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt
    14521 gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta
    14581 cttctttgaa attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca
    14641 accaggtgta gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga
    14701 agatcttgct aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa
    14761 taatcagtga tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca
    14821 aacatactgc atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct
    14881 tgtttgtata gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac
    14941 agtgaggctg acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa
    15001 aagcactcac ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt
    15061 tcttttacag atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt
    15121 tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca
    15181 ccagaagtaa tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct
    15241 acgttgtatt taaaacctct tccaattact tccccattat ctgctttgat atcagtttct
    15301 gcaggcattg gagctttgac taccgcataa taatctttac ctacataagt agactgatag
    15361 tagtcccaac ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt
    15421 tttttcatga aagatctcct tttttatttt tat
//
LOCUS       DADJYG010000062        15135 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.062, whole genome shotgun sequence.
ACCESSION   DADJYG010000062 DADJYG010000000
VERSION     DADJYG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.062"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(109..489)
                     /gene="yajC"
                     /locus_tag="JZ382_001510"
     CDS             complement(109..489)
                     /gene="yajC"
                     /locus_tag="JZ382_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY2427702.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(586..1731)
                     /gene="tgt"
                     /locus_tag="JZ382_001511"
     CDS             complement(586..1731)
                     /gene="tgt"
                     /locus_tag="JZ382_001511"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY2427703.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1949..2647
                     /locus_tag="JZ382_001512"
     CDS             1949..2647
                     /locus_tag="JZ382_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAY2427704.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2815..5454)
                     /locus_tag="JZ382_001513"
     CDS             complement(2815..5454)
                     /locus_tag="JZ382_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAY2427705.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6073..7104)
                     /gene="queA"
                     /locus_tag="JZ382_001514"
     CDS             complement(6073..7104)
                     /gene="queA"
                     /locus_tag="JZ382_001514"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY2427706.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7297..8472
                     /locus_tag="JZ382_001515"
     CDS             7297..8472
                     /locus_tag="JZ382_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY2427707.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8476..9117
                     /locus_tag="JZ382_001516"
     CDS             8476..9117
                     /locus_tag="JZ382_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427708.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9114..10031
                     /locus_tag="JZ382_001517"
     CDS             9114..10031
                     /locus_tag="JZ382_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427709.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10028..10699
                     /locus_tag="JZ382_001518"
     CDS             10028..10699
                     /locus_tag="JZ382_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427710.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11240..11728)
                     /locus_tag="JZ382_001519"
     CDS             complement(11240..11728)
                     /locus_tag="JZ382_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAY2427711.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11929..12195)
                     /locus_tag="JZ382_001520"
     CDS             complement(11929..12195)
                     /locus_tag="JZ382_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAY2427712.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12195..12458)
                     /locus_tag="JZ382_001521"
     CDS             complement(12195..12458)
                     /locus_tag="JZ382_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY2427713.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12451..12723)
                     /locus_tag="JZ382_001522"
     CDS             complement(12451..12723)
                     /locus_tag="JZ382_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427714.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12934..13119
                     /locus_tag="JZ382_001523"
     CDS             12934..13119
                     /locus_tag="JZ382_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2427715.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13261..15084)
                     /locus_tag="JZ382_001524"
     CDS             complement(13261..15084)
                     /locus_tag="JZ382_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAY2427716.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT         4386 a         3245 c         2853 g         4651 t
ORIGIN      
        1 attcaatatt gagcaatata tcccatgctc ttatctcttt cttttttgaa taaaaaatga
       61 ggtaggacat ctttctgccc tacctcgtct ttaaataagc tttgatactt atttttcttc
      121 cttctcttct gtctcttcca gcgtttctgt tgtttcagtt tttacttcaa ctgtctcagc
      181 tggttcttct gttgtttcaa cagtagtcgt tgcatcattt gttacgacag tccctggttt
      241 gaccgttttg attgctgcac gatcgtactc aaggaagatc ccttcgcagt ctaaaacaac
      301 tgtccgtttg tcattgtcga tttctgaaac aactccatgt aatccgccga tcgttacgac
      361 tgaatcacct ggtttcatgc tatctaacaa gttttgacgt tcttgctgtt gtttcttttg
      421 cgaacgtgac ataaaaaacc acattgctaa tagcgcgata aacatcacaa gcattggtaa
      481 tgttcccatt tttttcacct cactaattct gtatctacaa aaaacacttt agcagaaaaa
      541 tgctgataac actagttttt tacttaattt ttaaaagatt ttcctttaga agctttttgc
      601 gttttcttta ttgaacccgt attcttcaaa aaaggcttct ctaaattcaa gcaaattatc
      661 atccatgatt gcttgacgta cttgtttcat tagattcaac aagaagtata aattgtggta
      721 agaagtcaaa cgaatcccaa atgtttcatc acatttgatc aaatggcgga tataggcacg
      781 tgtatagttt ttacatgtat aacaatcaca tttctcatca attggacgga aatcatgggc
      841 atatttcgca tttttcacta ctagacggcc ttgagaagtc atacatgtgc catttcgagc
      901 gattcgagta ggcaagacac agtcaaacat gtctactcca cggataacac catcgatcaa
      961 tgaatcagca gcaccgacac ccatcaagta acgtggtttg ttttcgggga tcaacggtgt
     1021 agtgaattct aatacacgat tcatttctga tttaggttcc ccgacagata acccgccgat
     1081 tgagtaacca gggaaatcca tcccgactag atctttcgcg ctttgacgac gaagatcttc
     1141 aaaccctgct ccttggatga ttccaaataa tccttgacgg tctggattag catgagcttt
     1201 caatcctcgt tctgcccatc gagacgtacg ttctactgat tttttcacgt agtcgtaact
     1261 ttcatcgaat ggtggacatt cgtcaaaact catcatgata tcagagccca atttattttg
     1321 gatgttgatt gctttttctg gtgataaaaa catacgcgaa ccgttcaagt gatttttgaa
     1381 atgcacgcct tcttcttcga tattccgcat atcacttaat gaaaaaactt gaaagccgcc
     1441 tgaatctgtt aaaatcggtt gatcccagtt cataaattta tgcagtccgc cagcttcttc
     1501 tactaagtct tcaccagggc gtagccataa atgatatgta ttacttaaaa taacgccagc
     1561 acccatttct ttcaactctt ctggggacat tgtttttact gtagctaatg ttcctactgg
     1621 catgaacatt ggtgtgggga aagttccgtg tggcgtgatc aattccccta aacgcgcgcc
     1681 agtatgtttt tctttcttga ttaaacgata gcgaatggca ggttgcgtca ttcatttccc
     1741 tactttctat ataaaatatc ttgacaactc ccaccatcat aatcgtttca agtgcttttt
     1801 gcaagttccc tcggcttcct tctactttat ttaatattct tttttttatc ctttttttat
     1861 gaataacaaa aaaaagaaaa acattatgac agaaatattt tttgatacat ggtatattgg
     1921 tgttattaaa gaaaaggagg gaaaaatcat gcaatcttct gcagaattaa aacgccaagc
     1981 aaaagagagt ctaaaaggac gttggggaca agctgtttta ctaaatctaa tccctacatt
     2041 gattggtatt gctactattc ttcttattat gattcctatt atcttgtttg ccaccaccat
     2101 atatgatgga tcagcgagcg tctcagaagc gacaacttca ggaggcggta gttcaggagg
     2161 cggtggcgga attatttcca gcatcattag cgctttgttc atgagcggta tttcttggac
     2221 ttatcttgat attttacgtg gacaaaaaga tcagatcgaa ccattcaaag atgcatttcg
     2281 tggattcaaa ggtttgttta tcggcggagt gatcagctta gctgtgctga tctcgatctt
     2341 tacaacatta tggggtttgc tattagttat tcctggaatc attaaaagct attcttattc
     2401 tcaaagctat tttatttact acgatatcgt ccaagaaaca ggacaaacac ctaaagtact
     2461 ggatacgatc actgcaagtc gaaaattgat ggatggttat aaaggacaac ttttctggtt
     2521 agatctttca tttatcggct ggcatatctt agcattagca actgctggta ttggttatct
     2581 atggctcagc ccatacattt ctgcaacaaa agcagcattt tatgaacaat tgccaaaaga
     2641 tatttaaaag agagtgtgac ttgtgaccga gggggtggca ggagaacacc gtttatcgag
     2701 aggcagtaaa agaaacattc agcccattag caaaaaggag gtgtgacaac attcatgtca
     2761 cacctccttt ttgtatagtt ccagtaaaaa gaaaatcaac ttagaaacag ttaatcattc
     2821 ctcttcttct gcttccggta atcgatggaa cttgatcagt tcattcaccg cgattggtac
     2881 gatagataaa atccctacca agaaccagtg attcaaactg atgtgtacta ggccgaatag
     2941 ttcttgcgta aatggcagca aagcaacagc cgttgtaata gctaatgcca gtaacgccat
     3001 ttctaccaaa gctttattgg tcgctaaatt gactttgaag atcgattggc tgctgcgtac
     3061 attgaacaca tgcagaatcg atgaataagc taagatcaag aatgcaacag tttgaccgac
     3121 atgctgactt gcgtctaccc atgggctgat agtatcgaca taactaccta aataataacc
     3181 aaacaaggtt acgatggtga agacccctgc atttatggca attttttgca ataaacctcg
     3241 agcaaagatc ccttcatttt tcggaatggg tggctgatcc atgatgccgt gttccgctgg
     3301 ctctcttcct aaagcaaatc ctggaagtcc atcagcaact acattgacaa acagtagttg
     3361 cacagctgta aatggtgctc cccatcctaa cagcatcgca atcaaaacaa taaagatctc
     3421 cgaaatatta cagcttagca aaaagttgat agctttacga atattctgat aaaccgaacg
     3481 tccttgactg acagcgtcta cgatcgtcgc aaagttgtca tccgtcaaaa tcatatctgc
     3541 ggcactttgc gccacatctg ttcctgtgat ccccatggca cacccaacgt tacttgcttt
     3601 caacgctggt gcatcattca caccatcccc agtcatcgct acgactgcac ccgtacgttg
     3661 ccaagatttg acgatccgga tcttgtcttc tggcgttacc cgagcataga cagagagagc
     3721 tttgacacgc gcatctaatt cttcgtctga taattgatgc agttctgatc ctgaaagtgc
     3781 ttctgacgga tcattcaaga tacctaattc tttcgctatg gcactggcag tcaccacatg
     3841 atctccagtg atcatgactg ttttgatgcc agcctttttc gctcgcttga ttgctccttt
     3901 actttctgga cgtggtggat cgatcattcc gatcagacca atcaaacgta agtctttttc
     3961 cagtgcttca gaagaaatat cttgcggttc ttcgtcataa gtagcataac caacagcaat
     4021 cactctcagt gcccgtttac caaagcgatc attaacgatc gccgcttgtt ctacatcgcc
     4081 tgaactaaag cgcggagcaa gaacatcgaa tgctcctttg gtgattgaga tgtatttttc
     4141 gcccatttgg tgaactgttg tcatcatttt tcgttcagaa tcaaaaggaa gctcgtttac
     4201 tcgtggatat ttttcttcta attctgcctt tgtatgataa ttctcttcaa cagcccttac
     4261 gatggctact tctgttggat tacctttgat aacaaggtcg tcatcttctt gctcgaccgt
     4321 cacatctgta catagtgcag ccattttcaa tatttccatc gcgctgttcg tcattgaatc
     4381 ttcgatattc gttacttcat cttctttgct ccatacccgg cggacgcgca tttcattttg
     4441 tgtcagcgtt cctgttttgt ctgagcagat aacattagcg gttcctagcg tttcaactgc
     4501 tggcaactgt cggatgatcg catgtttcct cgccatcttc tgcacaccgt atgcaagtgt
     4561 caacgtaaca ataacagtca aggtttccgg tacagctgca acagccaagg aaatcgaagt
     4621 catgaacatc tcaaggagtg gttctccttg cagttcacca atcacaaata ctaaagccgc
     4681 agccgctaaa gcgatcatac tgattcgttt gcctagctga ttcaatcgga cctgtaaagg
     4741 tgttttttgc atagtatttt cgttcaaaag atccgcgatc ttacccattt ctgtagccat
     4801 gccaatggca gtgacgatcg ctttcccacg acctgcagca accgtacatc ctttaaatac
     4861 catattaagt tggtcaccta atgactcatc ttcttcagca ataaatgctg catctttttc
     4921 aactgcttca ctttctccag tcaacgccga ttcttctaca cgtaattgtg atgcttcgat
     4981 caaacgggca tcggcagaaa tagcatcacc ggactctaaa acaagaatat cacccactac
     5041 cagttgctct gactcaacag tggtcacttt gccgtctcgt atgacagttg ccgtttgttt
     5101 attcatatct tgcaaggatt ccagtgcttt ttctgcgttt ccttcttgga caatcgccaa
     5161 taccgagttg atcacaacga tagcgatgat cagcagtcct tcaaataaat ccccatgttc
     5221 tgtagcaaat gcggtataaa aggaaatcac cgctgctaca agaaggatga tcgttgtaaa
     5281 atcggacaag cttcgaaaga attttttgat catcgattct ttcggcgcct catcgaattt
     5341 attcaatccc tgcttttttc gccgttcatc cacttcttgt gaagacaacc cagtatctgg
     5401 atctgcttct gttaccttct ttacttcttc tattgtctgc ttgtattctt ccatcctatt
     5461 cctcctttaa aatcttcgta caaaaaaagg tacttatttt actataacat ccttttttta
     5521 tttacttcaa ataaagggct tatcgattat ttgttaagaa agcgtaaaat ttaaagtgct
     5581 tatatttgca aacgctacca aaggatgcta ttatatagat gtaaggaagg aggataccat
     5641 caaatagaca gaagaatttc tcgatagaac agtgtaagca aacaaagttg ttagaagtac
     5701 aggtttcacc cagacatgcg tgtagagtac aaacctataa atagtgaatg cgtatggatg
     5761 tttggatcac gaagtatccg aaacaagaag taatgcgata cgtaacaatt gaataacgta
     5821 aacgtctgaa aattgaatag gaacaagaga tgtttcaaat gttgaagatg taacgaaaac
     5881 aaaatttttg tttaacatga agaaattcaa caccgtgctt tgcatgtatg atggaaagtt
     5941 acaatcattt ctgtttattt gaatccttcc gaggtacaag aaagccgagc ttatcgagag
     6001 ctcggctttt tctttgtcat aaaatctttg taaagctttt ttgttattca aaactagcgg
     6061 cttttcataa ccttatttca cgaacatcgc atctccaaaa ctaaagaaac gatagcgctc
     6121 ttcaatcgca tgctggtaag cagataaagt caattctctt cctgcaaagg cactaactaa
     6181 catgactaaa gtcgattttg gcaaatggaa attagttgag aaagcttcta ccactttgaa
     6241 ttcatagcca ggcgtaatga aaatatcagt ccatccgctg tctgctttga tttctccatt
     6301 aaatttcgtt ccaatcgttt ctaatgttcg aattgaagtt gttccgacag ccacgatttt
     6361 cccgccattt ttacgtactt ggttcaatcg ctcagccgct tcttccgtca aacggtagaa
     6421 ttcactatgc atatggtgtt cttcaatttt atctacgctg acaggtcgga atgtacctaa
     6481 ccctacatgt agtgtcagat agactaattc aacacccttt gcttcgatct catcaagtaa
     6541 ttctttcgta aaatgcaatc cagctgttgg cgctgcagca gaaccatttt ctttcgcata
     6601 gacagtttga tagcgatctg ggtcttccag acgttctttg atatatggag gcaatggcat
     6661 ttctcccaat gattcaagaa tctctagaaa aattccttca tactcaaagt caatgatccg
     6721 tccgccgtgt tccaattctt ctttcactac tgcttgcaat ctgccatcac caaaagagat
     6781 tcttgtccca actttggcac gtttcgcagg tttgatcaat gtctcccacg tatctccttg
     6841 tgtattcgtt agaagaagga cttctaaatg cgcaccagta tctggttttt ctccatataa
     6901 acgagcaggc agaactcgtg tattattcat aactagcgca tcaccaggct gtaactcttc
     6961 aatgatttca tggaaatgtt tatcttccat ctctccagtt ttccgatcca aaacaagtaa
     7021 acgagaatga tcgcggtctt ttaatggtgt ttgtgcgatt aattcttccg gcaagtcaaa
     7081 atcaaaatct tctgttgtta acatatcgtc actcactttc tatcatgtgc ttcatcattc
     7141 tatcatgttt ttcgttctgt tggcaaaatc agcacttttt cacttctatt ttttgttttc
     7201 ctaaataaaa aaactaccct atttgtattt tttcattgtt ttaggcatac tagtaataga
     7261 tagttcttca ttgaaaatag aaaaagaggt ggcaccatga ttgaatttca aaatgtgtca
     7321 aaaatttata aaggtggaaa agtcgctgta gaggacgtca atctttcatt tgaaaagggt
     7381 gagttcattt gttttatcgg tacgagcgga agcggaaaaa cgacgacgat gcggatgatc
     7441 aaccgaatga ctgatccaac acaagggaaa attttgattg atggaaaatc catccaagaa
     7501 attgatccag tggaattgcg acgtcagatc ggctatgtta tccaaagtat tggattgatg
     7561 cctcacatga cgatccgtga aaatatcact cttgtccaaa aattgttgaa agtcagtcaa
     7621 gaagaacgga ataaaacagc tgaaaaaatg attgatcttg tggaattgcc ccgtgagatg
     7681 ttagatcgct acccacatga attatctggt ggtcagcagc agcgtattgg agtcgttcgt
     7741 gcccttgcag cgaatcaaga tatcatcttg atggacgagc cattcggtgc gcttgatcca
     7801 atcactcgtg attccttgca agaccttgtc aaagatttac aagagcgttt gggaaaaacg
     7861 atcgtatttg taacccatga catggatgaa gcgattaaac tagcaagccg tatcgccatc
     7921 atgagtgaag gccgtgtgat ccaatttgat acaccagaaa atattttacg tcatcctgca
     7981 aacgatttcg tagaagagtt gattggagaa gatcgcctac tacaagccaa gccaaatgcg
     8041 acaagagtcg gcgaagttat gctaaataca gcaatcacga ttacaccgga aaaatcatta
     8101 caagaagcaa tcaaactgat gagggaaaaa cgtgtagata cgttgttagt taccgataat
     8161 tcgaatgtat taaaaggatt tatcgatgta gaaacgttga ataaaaaacg aggaaaagta
     8221 tccagtgtcg gcgacatttt gaataaacat gttttttacg taaaagaatc cgcctatctt
     8281 cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat acgtgccggt cgttgatgaa
     8341 cagaacaaag tcgtaggtat cctgactcgt gcatcattag tggatatcgt ctacgatgtg
     8401 atttggggag aagatcccgc aactactgat gttgcagagt cagctactcc tgaagaatct
     8461 aaggaggtct gacctatgca gcagtttttt catcagcacg gttcagaaat tctctcaaaa
     8521 ggttttgaac atctttatat ttcaggactt gctttgatca ttggtatcat cattgctgta
     8581 ccgcttggtg tcttactgac acgttttcca aaagctgcca cgattgtgat tggcttaacc
     8641 agtgccttgc agacaatccc ttctcttgct ttattggcat tgatgatccc gctatttggc
     8701 gtaggtaaat tacctgcgat tatcgcactc tttatttatt cattattgcc gattttacgt
     8761 aatacgtaca ttgggatgaa aaatgtcgat tggaattatc gggatgtggc aaaaggcatg
     8821 gggatgactg agttccaatc cattttttcc gttgaattgc ccattgctat gccaacgatc
     8881 atggctggta ttcgtttagc tgccgtttac gtgattgcat gggctacact tgcctcttat
     8941 atcggcgctg gtggtttagg cgatttgatt tttagtggac tgaataatta ccaaccagat
     9001 ctcattttgg caggtacgat ccctgtaaca attttagcac tattggctga ttggcttcta
     9061 ggtctcttgg aacaccggct gacaccgatc gcacttagag aggagaatga agaatgaaac
     9121 gtatactaaa aatgaccatg gttcttttct ctcttcttat acttgcgagt tgttccttcc
     9181 ctggtcttgc cagcaataca gatgaagaca ccatttcgat cacaggtgga atcacttcag
     9241 aagcacagat cttagcaagt ctagttgccg gaatggttga acattataca gataaaaata
     9301 ccgcaatcat caacaatctg gctactacaa ctatcaacca tcaagcgatg atgaacggag
     9361 atgcttcaat ttcagcggct cgctacacag gaacagattt aacgactaca ttgaaccttc
     9421 ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa agatgaattt gagaaaagat
     9481 acggtcaaac gtggttcccg tcctatagtt ttgaaaatac gtatgtcttt cttgttcgaa
     9541 aagacaccgc acaaaaatac catctatcta aagtcagcga tctgaaaaat gtagcggatg
     9601 aattagtagc tggtgtagat acttcttgga tcaatcgtaa aggagacggt tatgacggct
     9661 tccaagaaac ttatggcttc tccttcaatt cgatattacc tatgcagatt ggacttgttt
     9721 atgacgcagt cgaagcagga aaaatggata ttgttcttgg ctactctacg gatggacgga
     9781 tcgccagtta tgatctagtc atgttggaag atgatcgtca attcttccca ccatatgatg
     9841 ctgctccagt tgtagacaac aagatattag ctgatacgcc tcatttaaaa gaagccttaa
     9901 ataagttagg tcataccatc agtacggaaa aaatgcagga attgaactac gaagcagata
     9961 ataatctagt ggaaccatca gtagtagcac aacgtttctt acaagaaaac cactattttg
    10021 aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa caatttttct attatttcca
    10081 ggaaaatggc tcctatatct ttgcacaatt tatccgtcac ttcctgattt ctatctatgg
    10141 tgtcctcttt gcagcagttg tcgggattcc ggttgggatc atgatttcta ggagacgtaa
    10201 attagcaaac tgggtgattc gtttggcgaa tatcatccag acgatccctt ctctagccat
    10261 gatctccatt ttaatcattg gattaggcct gggagtcaat gtcgtgattg ttactgtatt
    10321 tttgtattcc ctcttaccga tcatcaagaa tacgtatacc gggatgatcc aagtagacaa
    10381 aaacatcctt gatgtcggca aaggcatggg gatgactgct agacaacgac tattcatggt
    10441 agagttacca ctctctgttt ctgttatcat ggcaggcata agaaacgcgt tagttgttgc
    10501 catcgggata accgcgatcg gtgcattcgt cggtgctggt ggtcttggag atatcattat
    10561 ccgaggaacc aatgcaacag agggcacatc gattatttta gcaggtgcat tgccaactgc
    10621 attaatggcg atcatcacgg actggctttt aggtattttg gaaagacggc tagatccagc
    10681 cagccgtaca tcacgctgat gttattgtaa aaaacaaaag cttctgcgac catttgttgg
    10741 tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata atccactctg attcttccct
    10801 ccttatgggc aaagagttgc ttttcttagc tatttatcca ttccgtatac tccaacttca
    10861 tactgccaac gggttcatat ctgcatccac cgactaagtt tttttcttat ccgctaaggc
    10921 attttgccaa tccattttcc catcactcgt catggaattc ataccacatg atctctaaat
    10981 actcgtctgc ttgttctttg aattgggcat caattcttac atcctttggc gtggctttca
    11041 tttaagcctt ttccatcagt cctcgattgt tcgatgcctt cagctgcatc atcaaaactg
    11101 gtgactgccg tatcggtaac ttcgatcatc ttttatacaa tcgggaactt gccatcctca
    11161 tccttggcta aaaacatatc tagccattcc agcttctcgc catgggaggt ttgcggttcc
    11221 taaaaccggt acacgattct tatggactgc atccgtcgca actagacttg gcgatacgat
    11281 gatccttcac cagaagagca cccccgtaaa ccaattgatc gatatattgc caataagaaa
    11341 agacattggc gtaaaagata tcgatccctc gtgatgcatt gccgttgata ctgctattca
    11401 tggccgaaag cgccacgact ttaacttctt ccgtttgagt gacattcatg ttcggcaatg
    11461 tcgctttgga tacccgctct gctaaaggaa cgacactggt attgtatttc gtatctgtat
    11521 cttttccaaa agaccatgcc aacgaatcct ctgggaacca ataagacgac tccggttgat
    11581 tgtccattgc ttttttcaac atacttttgg ctgtttctcg ataaacgaca tttccaattg
    11641 tcttcgcctc actttggcga taatcgatgg tcgttgccac tgccaacact aacaacgatg
    11701 ctccaatttt actgattttc cttgtcacac ttcttcctcc ttgttttaca taaacccgtc
    11761 aacattccat tcctaaactc aaacaatgac ccttcaaagg tggagatata ggaacaaatt
    11821 aaagaaatca tgcaataaat ccaagaaggt tggttttaga acaaaaaaag agtctccttt
    11881 ttggggagac tctttgttaa ctatctaaaa aggttggctc ttctcacttc actcataatg
    11941 tgtccaggca gaataaattt ctacaacttt attttcctca tcaactttgt aaaccactcg
    12001 atgctgtcta ttcagcctgc gtgagtatct gccttcatgt gtaggtctca gcttttcaaa
    12061 agactgcgtg ggcatatatg gatcttcctt caatgtctga atcacttctt caaattgttt
    12121 ctttaaattc gtttgcttga tttttcttaa atccactttc gcagagtttt tgattgcgac
    12181 tgtatagtta ctcatcaaag agcttcccaa tcaatatcat cgatattcgt aaagctactg
    12241 tcgtcttttt cgcggtctcg tactttatct aaagtacctg tttgctcaag aaaaagggtt
    12301 tcttgaatcg ctctccagtc ttccagccca atcaatacag cattgtttcc actacgatca
    12361 ctaataatct cgatttcagt atgattttca tttacctctt tcaataactg ataaaaattt
    12421 ttacgagcag tgctaggatt catcgttgta ttagccattg tctctcctcc tttttctgat
    12481 ataattatag cgtacttgat taagtacgtc aaaaaaatac aataaagcac ttaaattcag
    12541 caaacttttc ctctttttta catagagaaa aagacccctt tttagggagg atctttgtcc
    12601 acgctcactt cttactcctt tacgactagc ggtagccaaa cgtaaagtaa ttggtaacaa
    12661 cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag tctatgcaac aacaccaata
    12721 cacgaaggtt cctcgttgca gtgtgacttg attgatatct tgaataacct gaggatacgc
    12781 acgaaagatt tgtaacggtt attcaaaact gattttggcc gtcaagtctt ctactgcctc
    12841 aaaaatgatt gcctctttcg tctcctgtca ttcatgttca ctatcgattt gatacttgct
    12901 tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac tgagttatgg taacaaaaaa
    12961 tggtcacttg acgaggctga gattcctact tatgaagaca catggcactt aagagtcgag
    13021 gtaaaggaag aagctgaaaa tataggaggc tgtacattat ttggcaaagg attcggaaat
    13081 tatgatcaag acatcaaaat caaaatgttt tacagctaaa taggaagaat aataacattt
    13141 catacgctgt tcccatcttt aatggcttat agagggcacc tttagctgag aataagacaa
    13201 aacagaccct ctagcttcaa ttaagagcta gagggtctgt ttttgatttt aaaacttggt
    13261 ttatttcttc aaatggtagc tataggtgct gacaacgata ttttcacgcc aagacaatcg
    13321 caatcttaac cgcaaacttg tttggttatg gagaatattc tgccagcgtc tgtttggtac
    13381 aaattgagga atcaacacag tagttgtata gttttgtttc gctgcatttt tacttacgat
    13441 atctacataa cggataattg gattttcaat cgagcgataa gaagagtgga cgattgaaaa
    13501 acggacatct gggaaatgct ttttaaattc tgcttcgatt tctttttctt tttcgacatc
    13561 ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg atcgaacgtg cgtaattcaa
    13621 cgcaccgata tttacccgtg tcacattccc gactaataca agaacggtat tcccgtcata
    13681 atcatgtagt tttgcatcat tttctaaacg aagctgttct gctacttttt tataatgatc
    13741 atggatctta taaaaaatga acataacgat tggcataatg atgaagaaag gccaaatgtc
    13801 ccctaaccga tagacaaaca aaatcgcaat gattgcatat gagatgaacg cacccgtaat
    13861 attggcaatc gatttgctta accatctttt tccttctttt ttccatttga cgaccatccc
    13921 tgtttgagat aaagcaaatg gaatgaacac gccgatagag tataacggaa tcaagcgttc
    13981 tgttgagcca tggaaaataa acagcaatac gatcgaacct agcgctagtg tgataatccc
    14041 attggaatag cctagtcgat ccccacgatc ttgatacatg tgaggcatga atttatcttt
    14101 tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg gtgttagcag caactgctaa
    14161 aatcagcgct gtagcaaatt gaaggacgta atacaaaata ccatgtccaa agacagcttt
    14221 accaatttga gacaaaacag tgacttcttt ttcaggaacg atcccatacc aatagttgat
    14281 aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc atcatcgtca atgtagcagc
    14341 ggcatttttt gctcttggct tcttaaagaa tggtacagca ttactaatcg cctccacccc
    14401 agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc aaagcgatag aaacacccgg
    14461 cacagccgct cctggaagtg cagtagcttg caatggcaca gcacctgtca cgatattata
    14521 taaaccaaca acgatcagta acgagatcac aaggataaat gtgtacaccg gaaataatag
    14581 gaacgatgca gattccctca gcccacgaag attcagcatc atcagtagca aaacgattgt
    14641 cacagaaata gcgacttgat gaccgtagag tgctggcacc gctgatgtga tcgcttcagc
    14701 acctgctgag acagatactg ctactgtcaa catgtagtcg atcaataaag agccaccgga
    14761 aatcaagcca gcattctttc caagattctc actgctgacg acatatgctc ccccgccatg
    14821 aggataagca tggatgattt gacgatatga tagcgttaat gaaatcaata aaataataac
    14881 aaaagcggca atgggtagcg aataccagat ggctgcagca gacaaagcga ctaaaacgac
    14941 aacgatttgt tccgtaccat atgcaataga tgataacgca tcagacgaca ataatgccaa
    15001 agcagcaaat cgggttaatt tgtgctcatc attttcagca gattttaaag gcttacctac
    15061 taacaacctt tttaagtaat ccaatttcta aactccttta cacacaattt tacttcaagt
    15121 tttatagtcg aaaat
//
LOCUS       DADJYG010000063        14815 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.064, whole genome shotgun sequence.
ACCESSION   DADJYG010000063 DADJYG010000000
VERSION     DADJYG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.064"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(296..1024)
                     /locus_tag="JZ382_001525"
     CDS             complement(296..1024)
                     /locus_tag="JZ382_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2427717.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="JZ382_001526"
     CDS             complement(1002..2576)
                     /locus_tag="JZ382_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2427718.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="JZ382_001527"
     CDS             2805..3743
                     /locus_tag="JZ382_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427719.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="JZ382_001528"
     CDS             3755..4666
                     /locus_tag="JZ382_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427720.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="JZ382_001529"
     CDS             4690..6162
                     /locus_tag="JZ382_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2427721.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="JZ382_001530"
     CDS             6213..6824
                     /locus_tag="JZ382_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAY2427722.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="JZ382_001531"
     CDS             6854..8767
                     /locus_tag="JZ382_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY2427723.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="JZ382_001532"
     CDS             8895..11876
                     /locus_tag="JZ382_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAY2427724.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="JZ382_001533"
     CDS             11873..13534
                     /locus_tag="JZ382_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAY2427725.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="JZ382_001534"
     CDS             13574..14506
                     /locus_tag="JZ382_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAY2427726.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DADJYG010000064        14393 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.065, whole genome shotgun sequence.
ACCESSION   DADJYG010000064 DADJYG010000000
VERSION     DADJYG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.065"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(288..3941)
                     /gene="rpoC"
                     /locus_tag="JZ382_001535"
     CDS             complement(288..3941)
                     /gene="rpoC"
                     /locus_tag="JZ382_001535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY2427727.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(4020..7637)
                     /gene="rpoB"
                     /locus_tag="JZ382_001536"
     CDS             complement(4020..7637)
                     /gene="rpoB"
                     /locus_tag="JZ382_001536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY2427728.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8367..9335
                     /locus_tag="JZ382_001537"
     CDS             8367..9335
                     /locus_tag="JZ382_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2427729.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9353..10258
                     /locus_tag="JZ382_001538"
     CDS             9353..10258
                     /locus_tag="JZ382_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427730.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10255..11061
                     /locus_tag="JZ382_001539"
     CDS             10255..11061
                     /locus_tag="JZ382_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2427731.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11218..12186
                     /locus_tag="JZ382_001540"
     CDS             11218..12186
                     /locus_tag="JZ382_001540"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAY2427732.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12503..13027)
                     /locus_tag="JZ382_001541"
     CDS             complement(12503..13027)
                     /locus_tag="JZ382_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAY2427733.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13314..>14393
                     /gene="scm"
                     /locus_tag="JZ382_001542"
     CDS             13314..>14393
                     /gene="scm"
                     /locus_tag="JZ382_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAY2427734.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDS"
BASE COUNT         4081 a         3095 c         2824 g         4393 t
ORIGIN      
        1 tgtctactgg ttttgtttta ttcgaggaat tattcaataa tagctagcaa aatattaaaa
       61 ataaccgctc tatcttccca tcaatttatt ttgcattcaa aaaatgatct atctttactt
      121 ttgttgattg acttctcttg gaagatgatc gttccgatac cttaattgga tacgaaccga
      181 tgttgttaag atcctttccc acttttcttc tagttagaca aattatttta gacaaaccaa
      241 aaaagaggtt gtgacaataa tttgtcacaa cctctttttt aaaatatcta ttttgtttga
      301 ttgttcattg cgtcttctgc agccatttgc gcttcaatat ctgaaatact gtagacattt
      361 tcgcttgcta caccgacttc ttttggttcc atgtcacgat aacgttgcat accagtacca
      421 gctgggatga tcttacctat aataacattt tctttcaaac caagaagagg atctttctta
      481 ccgcggatcg cagcatccgt caagacacga gttgtttctt ggaatgatgc agcagataag
      541 aaactatttg tttctaatga tgcttttgtg atacctagta agacaggtcg agaagttgca
      601 ggaacgcctc ctgagactag cgtattgtag ttttgatctt taaagtctgc gatgtctaat
      661 aatgtacctg gtaagatatc tgtatcacct ggatccatta cacgaacttt acgcagcatt
      721 tgacgaacca ttacttcgat atgcttgtcg ccgatttcta ccccttgcat acggtaaaca
      781 cgttgtactt cacgaagcaa gtagttttca acagataata catcgcgaac ttgcaacaat
      841 tgttttggat cgattgatcc ttctgtcaat ggtgcaccac ggtggatcat atcgccttct
      901 gctactttca tacgtgctgt ataaggaaca gtatacgtac gtgtatctgt ttttcctttg
      961 attgttactt ctttggtacg tgttgcagga tcttctgaga tatcgatgac atcccctgtt
     1021 acttctgtga tcactgcttg ccctttcgga ttacgtgctt caaagatttc ttggacacga
     1081 ggaagacctt gagtgatatc gtctccggca accccacctg tatggaatgt acgcatcgtc
     1141 aactgagtac caggttcacc gattgattga gcggcgatcg taccaactgc ttctccgact
     1201 tcaacgtcag agccagttgc aaggttgcgt ccgtaacaat gtttgcagac accatgtttt
     1261 gtattacatg tgaagacaga acggattgtc actgtttcga cacctgcatc aacgatttgt
     1321 tttgcgatgt cttcggaaat caattggttt cctgcaatga tcaattcatt tgtttcagga
     1381 tgacgtactt ctttacgggt gtaacgtccc actaaacgat cttctaatga ttcgatgatt
     1441 tcatttccct cgcggatggc ttggatttca agacctcggt cagtaccgca atcttcttca
     1501 cggatgatta catcttgggc cacgtcaacc agacgacgag tcaagtaacc tgaatcggca
     1561 gtcttcaagg ctgtatcggt catcccttta cgagccccgt gagttgagat aaacatttcc
     1621 aagacggata gtccttcacg gaagttcgaa atgattggca attccatgat tcgaccatta
     1681 ggagcggcca tcaaaccacg cattccggca agctgtgtaa agttggaaat gttaccacga
     1741 gctccagaat cagacatcat gaagattgga tttttcgctt ccaaaccttc catcagtttt
     1801 tgttggatgc tgtcttttgc agcattccaa acagcaatga cacgttcata acgttcgtca
     1861 tctgtgatca gaccacgacg gaattgtttt gtaatagttt caacttgttt atgggcattt
     1921 tcaatgattt cttgtttttc attcagtaca ctgatgtcag cgatccctac tgtgataccg
     1981 gcatgtgtcg aatgtttgta ccctaagtct ttcatacggt caagcatctt agaagtttct
     2041 gttactttga atcgtttgaa gacttctgcg atgatgtttc ctaagttttt cttcttgaac
     2101 ggtaatacta attcttgctc tttgatatgt gcaggaatat cagtaccagc ttcaacaaag
     2161 tatttgtccg gtgtttggac ggtcaagttg aagtctgttg gttcattcag ataagggaat
     2221 tcaggaggca tgatctcgtt gaagatgatc ttaccaactg ttgtgatcat cgtacgttct
     2281 ttttgccatt cagtaaatgg tttttcacct aatgagttag ggttcacacc gatacgtgaa
     2341 tgtaaatgaa catatccgtt gcgccatgcg ataacggctt cgttttgatc tctgaagacc
     2401 attccttcgc cttcgcgtcc ttcttcttcc attgtcaagt agtagttacc tagaaccata
     2461 tcttgagaag gagtaacaac tggtttacca tctttagggt tcaagatgtt ttgagcagct
     2521 aacatcaaca tacgtgcttc tgcttgtgct tcttcattca atggaacgtg gacagccatt
     2581 tggtctccat cgaaatcggc attataagct tcacatacaa gtggatgcaa acggattgcg
     2641 cgtccttgta ctaaaactgg ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc
     2701 cggttcagca atactgggtg ttccttgatt acgtcttcta aaacatccca tacttcgtct
     2761 tcttgacgtt cgattttacg tttcgcattt ttgatgtttg aagctagttc gcgttgaaca
     2821 agctcacgca tcacgaatgg tttgaataat tcaatggcca tttcttttgg aagtccacat
     2881 tggtacatct tcaagaaagg tccaacaacg ataacggaac gacctgagta gtcgacacgt
     2941 ttacctagca agttttgacg gaaacgacct tgtttccctt tcaacatatg agaaagagat
     3001 ttcaatgggc ggttacctgg tccagtaact ggacgtccgc gacgaccgtt atcgatcaat
     3061 gcatctactg cttcttgcag catacgtttt tcattttgaa cgatgattcc aggtgcattc
     3121 aagtctaaca aacgtttcaa acggttatta cggttgatta cacgacggta aagatcattc
     3181 aagtcacttg ttgcaaaacg tccaccttct aattgaacca ttgggcgtaa atctggtgga
     3241 ataacaggga taacgtccat aaccatccag cttggttgat ttcctgaagc gcggaatgct
     3301 tctaaaatat ccaaacggcg gattgcacgt gtacgttttt gtccttgtgc agatttcaat
     3361 tcttctttca attcggctgc ttctgcatct agatcaacat tatcaagtaa ttgtttaatc
     3421 gcttctgcac ccatagcagc ttggaattct tgtccatatt gttcacgttt ttcgcgatat
     3481 tcgcgttctg tcaacaattg ttttttctct aaattcgtat ttcctggttc gatgactaca
     3541 tatgaagcaa aataaatgat ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc
     3601 atacgtgaag gaatcccttt aaagtaccag atatgtgaaa caggagctgc taattcaata
     3661 tgtcccatgc gttcacggcg aactttcgaa cgagtaactt ccactccgca tcgatcacaa
     3721 acgattcctt tataacggat acgtttgtat tttccgcagg cacattccca gtcttttgta
     3781 ggaccaaaaa tccgctcaca gaacaaccct tcacgttcag gttttaaggt acgatagtta
     3841 atcgtctcag gtttttttac ttcaccgtaa gaccagcttc tgattttttc gggagaagcc
     3901 aaacctattt gcatactttc gaatttattt acatcgatca aaaggggttc cctccatttc
     3961 atttaatggc aaaccgcaag acggctgatc ttcgaccgcc ttgcagctca tagaccggat
     4021 tagtctaaat tatcttctgc ggtttcaaag ttttggatga catcgtcttt ttcatcttcc
     4081 acttgttcag cagctttctt ttctaattct ttggcagttt gctgttcggc aaattttgtc
     4141 aaagcatcaa cagtgatcaa atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct
     4201 gtttcttcga tatccaagac acgcatgtcc agacctagtg attgcaattc tttcaccaat
     4261 acgcggaatg attccggtac acctggtttt ggaattggtt cacccttgac gattgcttcg
     4321 tatgttttca cacgtccgac tacgtcatct gatttgtatg tcaagatttc ttgtaaagta
     4381 taagccgcac cgtaagcttc cagtgcccat acttccattt caccaaaacg ttgtccacca
     4441 aattgagctt tacctcccaa tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt
     4501 gcatgcaatt tgtcatcgac catgtgagca agtttgatca tgtacatcac accgacagac
     4561 acacgtccat caaatggttc acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg
     4621 gccataccgg cttcacgaac tgtttcccat acgtcttcat cacttgcacc atcaaatact
     4681 ggcgttgcta cgtggatacc taactgacgt gcagccatac ccaagtgtaa ttccaatact
     4741 tgcccgatgt tcatacgtga aggcacccct aaaggattca gcatgatatc gattggtgtt
     4801 ccatcaggta ggaatggcat atcttcttcc ggcataatac gggatacaac ccctttattt
     4861 ccgtgacgac cggccatttt atccccttca tggattttac gtttttgaac gatataaaca
     4921 cgaactaaca tgtttacgcc tggtgacaat tcatctccag cttcacgagt aaagattttc
     4981 acatcgtgaa cgataccgcc gccgccatgt ggtacacgta atgatgtatc gcggacttca
     5041 cgagcttttt cgccaaagat cgcatgcaat agacgttctt ctgcagataa ttctgttacc
     5101 ccttttggcg tgactttccc tacaagcaaa tcgccatctt ggacttctgc accaatacgg
     5161 atgataccca tttcgtctaa atcttttaat gcgtcttccc cgacattagg gatttcgcgt
     5221 gtgatttctt caggtcctaa ttttgtatcg cgggcttctg attcatattc ttcaatatgg
     5281 atagaagtgt atacgtcatc ttttaccaaa cgacggctca tgatgatcgc atcttcatag
     5341 ttgtaccctt cccaagtcat gaaaccaact aaaacgtttt ggcctagagc catttcgcct
     5401 tgttccatag atggtccgtc tgccagagta tccccttttt cgactttctc acccaaatga
     5461 acgattggac gttggttgta gcttgttccg gagtttgaac gacggaattt tgttacatcg
     5521 tatttatcta atgcaccgtt gtcgcggcgt acgcggattt cagaagcatc aacgtattca
     5581 acgacaccgt catgtttaca taacaaagca gctcctgagt catgcgctga tttgtattcc
     5641 ataccagtac caacccatgg tgcttgagga ttgatcaacg gcactgcttg acgctgcatg
     5701 ttggcaccca tcaaggcacg gttggagtca tcgttttcca agaaaggaat acatgctgtc
     5761 gcaacggcaa ctacttgttt aggtgatacg tccatgtagt ctactttatc aatagatact
     5821 tctaagtttt cactttgagc acgagccata acaacgtctt gagcaaatgt tccatcttca
     5881 ttcaatgggc tgtttgcttg ggctacgatg taatggtctt cgatatcagc tgtcaagtag
     5941 tcgatttggt cagtaacacg acctgtttca cggtctacac gacgataagg tgtttcgata
     6001 aatccgaatt tatttacttt cgcataactg gacaaactat tgatcaaccc gatgtttggg
     6061 ccttcaggag tttcgatcgg acacatacgg ccatagtggg agtagtgaac gtcacgaact
     6121 tcataaccgg cgcggtcacg agtcaatccg ccaggtccta aggctgaaag acgacgtttg
     6181 tgtgtcaatt cgccaagagg gtttgtttga tccatgaact gtgacaactg agaagaacca
     6241 aagaattctt tgatacttgc cacaactgga cggatattga tcaattgttg aggtgtcaat
     6301 gtttcagtat cttggatcga cattctttca cgaaccacac gttccatacg tgctagcccg
     6361 atacggaatt ggttttgtaa aagttcaccg actgaacgga tacgacgatt tcctaagtgg
     6421 tcgatatcat cgacattgcc gataccttcc atcaagttca agaaatagct cattgaagca
     6481 atgatgtcgg ctggacgaac cgtttttaca gctgcttctg gatagccgtt tccgatcacg
     6541 tttacttcac gttcagggtc tcttggtgaa aatactttga tgacttggac agtcattgga
     6601 tctgtgacca caccgtcttc tgatggatag taagtcactg aatttaggcc attatcaatg
     6661 aaaggagcta gtgtttccat gacttggtga gtcagaactg ttcctttttc aacgatgatc
     6721 tcgcctgttt ctggatcaac taatgtttca gctaatgtca gattcaatag acgtgttttc
     6781 aaatctaatt ttttgtttac tttgtaacga ccaacgtttg ctaagtcgta acgttttgga
     6841 tcgaagaaac gagcattcag caagttacgt gagctgtctg ctgtttttgg ttcgcctgga
     6901 cgcaaacgtt cgtaaacgtc tttcaaacct tcttctgttc ttgagtcgct tgcgttttta
     6961 tgcaagtctt tttcgactgt attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc
     7021 gtatcatcgg aaccaaatcc taacgcacga actaatacgg ttaaaggaat cttacgtgta
     7081 cggtcgatac gtacataaga gatgtctttt gcatcagttt ccatttctag ccaagctcca
     7141 cggttaggga taacagtaga accgaatcct tcttttccgt ttttgtcgac ttttccatgg
     7201 aaatagacac ctggtgaacg gactaactga gaaacgatta ctcgctctgc cccgttgatg
     7261 atgaatgttc cttgttctgt cattaatggg aaatcaccga agaatacttc ttgtgctttg
     7321 atttcgccag tctcgcggtt tgtcaaacgc aatgttacat gtaatggtgc agagtagttt
     7381 gcatcatgcg cacgagcttc tgctactgta tactttggtt ctttcaattc ataatctaca
     7441 aattccaaag atagattgcc attgaagtca tcgattggca aaatgtcttc aaacatttct
     7501 ctaagtccct catctaagaa ccattggtaa gagtctgttt gaatttcgat caaatttggt
     7561 aattccaaca cttcactgat tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt
     7621 actacgtgtc cagccaagct cttcacccct ctaaaatgtc attcaaaaag actacccgat
     7681 caattgttac aataatattt caaatattat ttttaggtaa caacagacat tttttgcggc
     7741 ttttaggcaa aaaaaaacca aaaatagaac attctcttga aaccttactt cgtgagcttc
     7801 tacttttgtt tccttatatt ttaaagccga aacggacaat atttcgtttc tgccttttct
     7861 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
     7921 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
     7981 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
     8041 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
     8101 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
     8161 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
     8221 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
     8281 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
     8341 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
     8401 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
     8461 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
     8521 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
     8581 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
     8641 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
     8701 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
     8761 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
     8821 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
     8881 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
     8941 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
     9001 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
     9061 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
     9121 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
     9181 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
     9241 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
     9301 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
     9361 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
     9421 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
     9481 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
     9541 ttgtcttcta ggctttttag ggggtgcggc agcagggctt gtttccggac tgcttcacac
     9601 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
     9661 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
     9721 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
     9781 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
     9841 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
     9901 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttacttgc
     9961 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    10021 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    10081 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    10141 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    10201 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    10261 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    10321 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    10381 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    10441 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    10501 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    10561 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    10621 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    10681 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    10741 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    10801 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    10861 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    10921 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    10981 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    11041 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    11101 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    11161 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    11221 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    11281 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    11341 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    11401 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    11461 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    11521 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    11581 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    11641 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    11701 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    11761 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    11821 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    11881 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    11941 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    12001 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    12061 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    12121 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    12181 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    12241 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    12301 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    12361 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    12421 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    12481 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    12541 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    12601 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    12661 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    12721 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    12781 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    12841 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    12901 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    12961 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    13021 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    13081 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    13141 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    13201 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    13261 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    13321 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    13381 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    13441 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    13501 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    13561 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    13621 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    13681 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    13741 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    13801 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    13861 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    13921 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    13981 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    14041 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    14101 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    14161 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    14221 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    14281 atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca agcgacagta
    14341 cagatacatc taccacaagt gatagctcag attcatcaac ttcaagcgac agt
//
LOCUS       DADJYG010000065        14086 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.066, whole genome shotgun sequence.
ACCESSION   DADJYG010000065 DADJYG010000000
VERSION     DADJYG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.066"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            297..1406
                     /locus_tag="JZ382_001543"
     CDS             297..1406
                     /locus_tag="JZ382_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY2427735.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="JZ382_001544"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="JZ382_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="JZ382_001545"
     CDS             2401..3225
                     /locus_tag="JZ382_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427736.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="JZ382_001546"
     CDS             3257..4567
                     /locus_tag="JZ382_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAY2427737.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="JZ382_001547"
     CDS             4715..5974
                     /locus_tag="JZ382_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2427738.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="JZ382_001548"
     CDS             5984..6856
                     /locus_tag="JZ382_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427739.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="JZ382_001549"
     CDS             6868..7698
                     /locus_tag="JZ382_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427740.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYTVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="JZ382_001550"
     CDS             7738..9921
                     /locus_tag="JZ382_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY2427741.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="JZ382_001551"
     CDS             9982..11202
                     /locus_tag="JZ382_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAY2427742.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="JZ382_001552"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="JZ382_001552"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY2427743.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="JZ382_001553"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="JZ382_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         4759 a         2166 c         2768 g         4393 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatac cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatt
//
LOCUS       DADJYG010000066        13824 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.067, whole genome shotgun sequence.
ACCESSION   DADJYG010000066 DADJYG010000000
VERSION     DADJYG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.067"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(50..910)
                     /locus_tag="JZ382_001554"
     CDS             complement(50..910)
                     /locus_tag="JZ382_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAY2427744.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            1002..1430
                     /locus_tag="JZ382_001555"
     CDS             1002..1430
                     /locus_tag="JZ382_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAY2427745.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1592..1768
                     /locus_tag="JZ382_001556"
     CDS             1592..1768
                     /locus_tag="JZ382_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY2427746.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            1795..2241
                     /locus_tag="JZ382_001557"
     CDS             1795..2241
                     /locus_tag="JZ382_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAY2427747.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(2395..3774)
                     /gene="brnQ"
                     /locus_tag="JZ382_001558"
     CDS             complement(2395..3774)
                     /gene="brnQ"
                     /locus_tag="JZ382_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAY2427748.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            4480..5451
                     /locus_tag="JZ382_001559"
     CDS             4480..5451
                     /locus_tag="JZ382_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY2427749.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            5475..7667
                     /locus_tag="JZ382_001560"
     CDS             5475..7667
                     /locus_tag="JZ382_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAY2427750.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            7684..8160
                     /gene="ybeY"
                     /locus_tag="JZ382_001561"
     CDS             7684..8160
                     /gene="ybeY"
                     /locus_tag="JZ382_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY2427751.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            8138..8539
                     /locus_tag="JZ382_001562"
     CDS             8138..8539
                     /locus_tag="JZ382_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAY2427752.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            8554..9453
                     /locus_tag="JZ382_001563"
     CDS             8554..9453
                     /locus_tag="JZ382_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY2427753.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            9596..10408
                     /gene="recO"
                     /locus_tag="JZ382_001564"
     CDS             9596..10408
                     /gene="recO"
                     /locus_tag="JZ382_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY2427754.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            10792..11709
                     /gene="glyQ"
                     /locus_tag="JZ382_001565"
     CDS             10792..11709
                     /gene="glyQ"
                     /locus_tag="JZ382_001565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY2427755.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            11711..13786
                     /locus_tag="JZ382_001566"
     CDS             11711..13786
                     /locus_tag="JZ382_001566"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY2427756.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         4654 a         2484 c         2896 g         3790 t
ORIGIN      
        1 aaatttcgtt gtcacactcc agtgtttttt aagaaaaaga caaaaatgat taatcgcttt
       61 tttctgttgt ttcagttcca taataactat gatcttctag gtctaaagca ttcaaaataa
      121 gcgaagccgt ttcttcaata gataaagatg ccacattgat cactacacat cctagttttt
      181 cataaagatc attcgcaaaa tcgagttcag ctctaatttt ttcgatatcg gaataagcag
      241 tatcagggtt taatccgtaa gagcgcatcc gttctttacg gatattattt aaaatatctg
      301 ggttattcgt tagtcccacg atttttttag gatcgatttc ccacagctgt ttgggaatat
      361 gtgcttgggg aatcaaaggt aaattagcaa cttttaaatt tttatttgct aaaaacaagc
      421 tcaaaggcgt tttggaagtt cgggatacgc ctagcaaaac gacatctgct tctaaaaaac
      481 cacgaggatc tttcccatca tcgtatttta cagcaaattc cattgctttg atccgtttga
      541 aatagttctc attcaataaa tggagggcac caggttgccg aattggcttg ataccagtta
      601 aacgttcaat ttctgatatc ggtggcgtca tcacatcgaa actaaataat tgatgttctt
      661 cacaaaaaga acgtgttatc tcgatcagtt cttgattgat taatgtatgc aggacaagtg
      721 ctttttcttc taatgcatct tgcaatgcct gccttaatgt tttttcatct gtcacgaacg
      781 ttctgcggaa taggttgaac tcaacggtag gatactgagc catagtagct tgcgctagtt
      841 ttgtcgcagt ttctccagca gagtcggaga tgacaaaaaa agtaacgatt tctgtagaat
      901 cggaagacat ggataaaacc tcacttgcat tttttttatg tttattatac catactctag
      961 aatgcgtact tcgtaatcat tttgatttgg aggaataagt catgaaaaaa cagacattga
     1021 tcgaacaatc attagaaaca ttgaaaaata acggttttaa atatactaaa aaaagagaag
     1081 cattgttaac ttatttagtg aagcgtaacc gctacgtatc tgctaaagaa gtttttgact
     1141 atatgaatca ggaattcaaa ggggtaagtt atgatacgat ttaccggaat ctccatgatt
     1201 ttgcagagat cgatttactg gaagaaaccg atttaaatgg tgaaatgaag tttcgttttc
     1261 attgctgtca gggggaaatc ggccatcacc atcattttat ctgtacagtg tgcggaacaa
     1321 cgaaagaaat ccatatgtgt ccaatgaatt atttcgaaga acagctaggt ggatgcacga
     1381 tcgaagggca tagattcgag attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat
     1441 aaaataacat ttgataattt aaaaaaaaag caagatttcc ttgacggtca aaacagcatt
     1501 agatataata tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt
     1561 tactttttaa gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga
     1621 atcacttgac gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca
     1681 agaatctcgt aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga
     1741 agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca
     1801 ctactaagta gattaaacga tgacatgaaa gcagcgatga aagcgaaaga taaggaatct
     1861 ttacaagtca tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac
     1921 gacttgacag aagaagaaga actaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat
     1981 tctttgcatg agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa
     2041 attgtaatag tcgaaaaata tatgccggaa caactttccg acgaagaaat tcgacaactt
     2101 gttcaagaag caatcactca aactggtgct tcatcaatga aagaatttgg caaagttatg
     2161 ggtgcaatta tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg
     2221 aaagaactat tacaatctta attttatcat ttaaactgcg gacagtgatt gtccgcagtt
     2281 ttttgtgttc gtaaaaaaag aactaaaaaa cagctagaag aaagaataag attctttctt
     2341 ctagctgttt ttttcctgtt taatatctct tatgttaaat tgtgtttgat ttggttattc
     2401 ttctggtagt tcatttgctt cttgtttata ccctgatagg cgataggcgt aataaaagag
     2461 taagctgcca gcgtaaccaa gtaacgttgg gaccatccag ccaagtccta atgcagcaaa
     2521 aggaacgtga tgatgataaa atcctagtaa gcttgctacg acagtttgct gactcactaa
     2581 tgcaggcgaa tttgccaaca tatccaatac ggctggaacg gcagaaaaga acatcgttac
     2641 ttgatagaca aatggactct tttggaagaa ttttgctgtt aaggaaagca agattaacgc
     2701 aagtgacaat ggatacaaaa gcatcaatac tggtaaagac cattggatga tattatccaa
     2761 tccggcattc gcaacgacaa aagagacaaa agtcgttaac cgcagccatg tcatataact
     2821 gactttaggg aacagtttat ggaaatcttg tgcaaaagaa acaacgagtc ccatagcggt
     2881 agtgaagaca cctaatgtga ccattacccc taagaagcta gcaccgaagt tacctaaata
     2941 aaaattagtg atctgagaaa gtgcagttcc gccattttct gataaaggca tcttattcaa
     3001 actcatcgca ccgagaagga ccagaccaaa ataaatcatg acttctaacg caacgctcag
     3061 gctaccagct tttgctgtta attctgtgac ttttttatct ttaaaaccaa gatcacgaac
     3121 tgcatggaca aaagtgacac ttaaagcaag taaagctact gcatcaaccg tgttgtatcc
     3181 ttctaaaacg ccatttgtca aagcgttcgt ctgataagaa tctgcagcag attggtttag
     3241 attccccatg ggttggaata aagaaacgac aaaaatgata gctaataatg caagaaacaa
     3301 ggagtttaaa tactttccga catatttgac caaacgtgtt ggatgtacag taaggaaata
     3361 agcaagtaaa aagaaaagag cagagaataa aaacatacct aatgaatcat atttttcagg
     3421 caaaaagggt ttagcagcca tttcaaaagc agttgctgct gtccgcggtg taccaaagaa
     3481 tggtccgatc gttaaatgaa caaggatcaa gaacaaggca gcataccatt ttccgacagg
     3541 tttagctaag tcatacagac catcactttt tgtcactact acagctaaga tagctaataa
     3601 tgggaaaaga gcgcctgaga tggcaaatcc tacaccagcg ctgatccagt ttgatccagc
     3661 catttgacct aagtggacag gaaagatcag attacctgac ccgaagaaca tcccgaagat
     3721 aagtgaactg accatcaata attgcttcca tgaataagtt tttgtttctt ccataaattt
     3781 ttcctccaat aattatcatt ttgagtaaca aaataaacgt tgcagaaact agaaatgcca
     3841 ctaatatcag attccatgaa aaccaccacc ttaaaatata aaaaaaagcc cttttccact
     3901 tgggaaaagg gctctagatt ctagaacggt accacctttt ttcgcttaaa aaagcttctt
     3961 cattcacgga cgcttttcga taaagttatc cgctagcctg ataatggagg cagccacaat
     4021 gatctacgtt ttcgacattg actcggaaat gatgttcgtc tattcgattt attgtcattt
     4081 ctcacttaca tgactcactg ggaataaatc gctgaatagc ctactgtttt cctcgacgct
     4141 ttgctgtata tttatttgtt gacgttataa tacacctgtt ttttttgaaa tgcaagaata
     4201 tttcaaatat tttgttttct ttttttgaaa caagaaaaat acccagagaa tacttgctaa
     4261 gttagacgga atacttgtcc tctatcttat gcatagctta catgatcagc tttttttatt
     4321 caaacgagac ctttagagaa tggaagcagc tattttttaa cgtttcttgg tacttatggt
     4381 taagcaactc ttttagaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa
     4441 ataggaaaga atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct
     4501 ttagagatta gattgacggc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag
     4561 catataaaac tgattgaaga aacaacagaa acaaccatcc atacacgtgg agaaatcatt
     4621 cagatcattg gcgaacaatc ccagatttcc ttggctgcct cagtcattcg gacattgcag
     4681 gaattgatca aacgaggaat ccatgtaagt acaccagatg tcgtgacggc actgaaaatg
     4741 gggcaaaaag gcacattaga ttattttgtt gaaatgtatg aggaagagat cgtcaaagac
     4801 cgtaatggaa aaccaatacg ggtaaaaaac agcggccaaa agaaatatgt cgaatcggta
     4861 gccaaacatg atatcgtatt tggcgtagga ccagccggga caggaaaaac ttttcttgca
     4921 gtagtattag caatcgctgc cttgaaaaaa ggtgaagtgc aaaaaatcat tttgacgcgg
     4981 cctgctgtgg aagcagggga aaatctaggc tttcttcccg gggatctaaa agaaaaagta
     5041 gatccttatc tgcgtcctgt ttatgatgct ttataccaaa tttttggatt agatcatacg
     5101 aaccgattga tggaacgagg cgtgatcgaa attgctcctt tagcttacat gcgggggcgg
     5161 acattggatg atgcattcgt tattttggat gaagcacaga acacgacagt tgctcagatg
     5221 aaaatgttcc tgacacgtct tggttatcaa tcaaaaatga ttgtcaatgg agatacgagt
     5281 cagattgatc tgcctcgagg gacaacaagt ggtcttgttc atgcagaacg aacattaaag
     5341 caaatcaaga aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta
     5401 gttgccgaaa tcatcaaagc ttatgaaaag gcagatctcc atcaagaata agctttgctt
     5461 aggaggataa gcacatgttg aataaaacac tgcagaaact acaagaaaaa cttggaaagg
     5521 cagcattgcc tttcctgctg ttcttgtttt ttatcattat ctgcggcgtt acatttagta
     5581 gtgtcaaaca aaaggccaat gatttcagag aaggccaagt agcagaagaa agtatccgtg
     5641 cgaacaaaac gatcgagaac acggaagaaa ccgaacaaaa aagaaaactg gcagcagaag
     5701 cagtcactcc agaatacact taccaaaaag atcttgcaga tgaccagaac aatcggatca
     5761 agcaactatt tgaattgatc gataaaacga atgatgccat caacaaatct tatgacgaaa
     5821 agattgataa ggcaaaggaa gatgaaaata tcccaaagcc tactacagaa gagcggatcg
     5881 ccgcgttgaa gaaaaatttt gaaaatgtga atgcagaaaa tgttgcgttt tatcaaaaac
     5941 taccgaacaa tttttattcg acgatatttg aaatgactga aactgagatc catactgtga
     6001 gggatgagag tctgaagctc attgacgaac agatgagcaa acaagttcgt gaaagtgaat
     6061 tggaagcttt taaacaagaa gcagaagagc aaatccaata tttgaatgtc acaccagcac
     6121 aacaacaaat gattcgctac ttagtggatc aaggtatcgt agtgaatgac gtattgaatg
     6181 agaaaaagac agaagaattg aaacaatccg cacgagaagc tgtacaacca gtgatgatct
     6241 tccaaggaga aatcattgtt cgtgaaggta atcaaatcga tgcttcagct atgaaaaaat
     6301 tggaattact aggactgact aatcaaacaa cttcgatttt tccgctaatt gccatggttt
     6361 tagctgtttt gcttcaaatc ggcgtactga tttataattc acttcaattc caaaatgcag
     6421 gagaaagaac aaaatacgtg cttttctatg tgacagctat gtcagtcagc atagtgctga
     6481 tgaaattttt ccaattattc caaacggaac aagctactta tatcccgctg ttttatcccg
     6541 ctgcttttgc accacttgtt ttaagctttt tcttgaaccg aagagcaggg atcatggctg
     6601 gtattttcca agcagtatct gcacttttca ttttttatga ttcgattggc acgaactttc
     6661 taacgattat cttgatgtct tatgttttct ctgggctcat ggcaacgatt gtcagacgca
     6721 aacggatctc agaacaagga ttatctgctg ccatgtgggt cattattttt cctgtgatga
     6781 tggatgtcat tttgatcgtc tatcaaggaa tgagctttgg tgatgcaacg acttggttaa
     6841 tgcttgtctg cgggttcact ggtgcactct tctcattctt gctgacgatc ggtctacacc
     6901 catatattga attgatggtc aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc
     6961 caaatcatcc tttattgaaa cagttgctgg aagaagcgcc aggtacttat catcatagta
     7021 tgatggtagc taatctaagt gctaacgctg tagcagaaat cggcggacgt tctttgctaa
     7081 ctcgagtagc ttgctattat catgatatcg gcaagatcaa acatgctagc tttttcgttg
     7141 agaacttgcc ttcaggagcg gaaaacccgc acaatttctt attgccagaa gacagtaaac
     7201 atattatctt tggacatgtg acagatggag ccaaaatatt ggaagattat aatatgccgc
     7261 aaatggtgat cgatatttgc aaacagcatc atggaaccac gctaatgcgt tatttctaca
     7321 tcaaagcgaa agaacgcaat ccggaagtaa cagaagagca attccgttat cctggaccaa
     7381 aaccgcaaag tcgtgaagca gggatcgtca atattgctga cagttgtgag gctgcagtac
     7441 gggcaatgga tcatccgacg agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag
     7501 agcgtatttc cgatggtcaa ttagacgata gtggactgac cttgaaagaa atccgaaaag
     7561 tggaaaaatc attgatcagc ggtttaagta gcaccttcca ttctcggatc aaatatccta
     7621 aaatgaaaac agaagttgag aagataaagg aagaacaaga agaatagaaa gaagcgatgt
     7681 ataatggata ttacctttat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa
     7741 atcgatgact tgctgcaatt tgcagcatct tatttaaagc tgccagagga aacagaaatg
     7801 tctgttactt tcatggacaa tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa
     7861 gatgcaccga cagacgtcat cagttttgct ctggaagaag aaggcgaaga cgaaatccca
     7921 gtgatttttg aagatggaga gaatccattg cctcgtgtac taggagacat catgatttct
     7981 attgaacgtg ctgctgaaca agcagaagct tacggccatt catatgatcg ggaacttggc
     8041 tttttagcgg tacatggatt cttgcatatc aacgggtatg accatatgac accagaagat
     8101 gagaaagaaa tgtttggttt acagaaagag atcttggatg cctatggact caaaagataa
     8161 aaacgtagag aaaaacaaac atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa
     8221 aaccgttttt gaggaagaac gcaatatgag gaaacatgtt gcgttcggta tccttgcttt
     8281 atttatgggc gtgattttcc agcttgatcg gatagagtgg ttatggctgc ttttagctgt
     8341 ttttctcgtt tggatagtag agatcatcaa tactgttttt gaaaatgtag tggacatgtt
     8401 tacggatttt cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt
     8461 tttagtgacc gcttgttttg ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata
     8521 tcatttgttg tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc
     8581 gcaatcgtcg gccgcccgaa tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa
     8641 aaaattgcca ttatgagtga caaggcacag acaactagaa ataaaatcca aggagtatat
     8701 actacaccag aagctcagct gatttttatc gatacaccag gaatccataa accaaagcat
     8761 cgtctaggag attttatggt agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc
     8821 ttatttatga tcagtgctga tcaaaaacgc ggccggggag acgattttat tattgaacgt
     8881 ctaaaaaacg tgcattctcc agtttatctg attatcaata aaatcgataa gatccatcca
     8941 gacgagttgc tgggaatcat cgaagattat tcgacgcaga tgccttttgc acaagtgatt
     9001 ccgatctctg ctacagaagg aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa
     9061 atgccagagg gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttt
     9121 atcgtttctg aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat
     9181 tctgtcgcag tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag
     9241 gccacgatca tcgtcgaaag agatagccaa aaaggaatca ttatcggcaa aggcggaaaa
     9301 atgctgaagc aagtaggaac gaaagcaaga aaggatatcg aaaatctttt aggagacaaa
     9361 gtatttctag aactttgggt caaagtccaa aaagactggc gagacaaaaa agtctatctg
     9421 caagactttg gctaccgaaa agacgaatac tgatattctg aacttcttat aggttaagta
     9481 tagaaataaa ctggataaaa atccgcacgt tgagaaaaac ggcggaagat cgaaacataa
     9541 ataatctgga tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag
     9601 ccagcttgca gaatcgaaag gcttgatttt atctagccgt aattacaaag aaaaagataa
     9661 attagtcaaa atctttacgg aatcttctgg caaaatgatg ttttatatca aaggaatcca
     9721 tcggcaaaat cagccattag ctccggcttt gctccccttt accgaagcag tctatatcgg
     9781 taattttcga agtgaaggtt tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg
     9841 gaacatccaa gaagatatct ttttgagcgg ttatggtacc tatttgctaa atctagtaga
     9901 tgcagcaatc gaagatcatc aatatgaccc gcatttattc caatttacgc aacaagctct
     9961 gcagcggatg gatcaaggag atgatgcaga aatcatcacg aatatctttg aagttcaaat
    10021 actgcaacgc ttcggaatcg cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa
    10081 aggaaaattt gactattctt ccaaatctgg cggtgtgata tgcgagaaac actggccaat
    10141 ggacgaacac cgttatcatg cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc
    10201 tatttcttat gataagatca gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg
    10261 tgacctcatt gaccagttgt atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt
    10321 cattgatcaa atgaagtctt gggcacacgt attaaagacg ccagagaaaa atatgcctga
    10381 aaaagagata cctgaaaaag aagaataaat gaaagataca acaaaaaaat caaaactatt
    10441 tgacaaatac agggacttcc gtctattatt aaccgtagat taaatagatc gttcactgag
    10501 aggatctatt atataaacaa gaaaactgca aaatgaatac gtacattgac caagatcagt
    10561 aaaaaacacg tggtttagcg agttcgggag agtggaagcc ggacccaaac gatttttgaa
    10621 gaggcacttg cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa
    10681 ccgcgaaatt attcgtccct atgtctgtaa tcagacatag ggactttttg tcgttttctt
    10741 tcctgtattc ttattgacag caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa
    10801 aaattgaccg tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggctgc
    10861 atgctgatgc aagcttatga cacagaaaaa ggagcaggta cgatgagccc atatacattc
    10921 ttacgggcaa tcggaccgga accatggaat gctgcttatg tagagccatc ccgccgacca
    10981 gctgacgggc gttatggaga aaacccaaac cgtttgtatc agcatcatca atttcaagtc
    11041 gtaatgaaac cttcacctga aaatatccaa gagctttatt tagaaagctt gcgtctttta
    11101 ggaattgatc cattagaaca cgatattcgt tttgttgaag acaactggga gaacccatcg
    11161 atggggtgtg ccggtgtggg ttgggaagta tggcttgatg ggatggagat cacacaattc
    11221 acgtacttcc agcaagtcgg aggactacct tgtaaaccag ttacttctga gatcacttat
    11281 ggactagaac gtcttgcttc atatattcaa gaagttgaaa gtgtctatga tctggaatgg
    11341 gctgatggtg tgaaatatgg cgagatcttc aaacaaccag aatatgaaca ttccaaatat
    11401 tcttttgaag taagtgacca agacttgcta ctaagcaact ttgaccgttt tgaaaaagaa
    11461 gctaaacgct gtatcgatga aaatcttgtt catccagcgt atgattatat tttgaaatgc
    11521 agccatacat tcaatttatt agatgctcgc ggtgcagtat ccgtaacaga acgagcaggt
    11581 tatctttccc gtatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc
    11641 gaaaaattag gattcccttt attgaataaa aacacagcaa atccagaaaa catgaaggag
    11701 gctgaataag atggcaaaag acttattact tgaaatcggg ctggaagaaa tgcctgctca
    11761 tgttgtcaca ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa
    11821 ccaattgact tatgatacga tcgaaacgtt ttctacaccg cgccgcttag caatcaaagt
    11881 aacaaacatt ccagaacgtc aagcagacag tgaagaagaa gtcaaaggac cagccaaaaa
    11941 aattgctttg gacgcagatg gaaactggac gaaagcagct gaaggatttg tccgcggaca
    12001 aggtctgacg acagatgcta tcacttttag agaattaaaa ggcgtggaat atgtctatgt
    12061 aacgaaatac actcacggca aagaagcgaa agaagtattg acaggtttag cagatgtcat
    12121 taaaagcctc actttcccag taacgatgca ttgggcagaa tacgattttg aatatatccg
    12181 ccctattcac tggatcgtgg cattgttaga tgacgaagta atcccgtttg agatcttaga
    12241 tgtcaaaaca ggcagagcct ctcgaggaca tcgtttctta ggcgaagatg tgacatttgc
    12301 gcatgcaaaa gaatatgaag aaaaattgaa tgaacaattc gttattgctt caccgcaaaa
    12361 aagacaagaa atgatcgtta cacaagcaaa tgaattagcc gaaaaaaatc actgggttat
    12421 tgatctagac gaagatttac tagaagaagt caacaattta gtcgaatatc caactgtctt
    12481 ttctggcgac ttcgaagaaa aatatctttc tgttcctgaa gaagtactag tcacttctat
    12541 gaaagaacat caacgttatt tcgaggtacg gaacgatcaa ggtatgctgc ttcctcattt
    12601 tatttctgct cgtaatggtg atcgggtaaa attggaaaat gtggttcgag gcaatcaaaa
    12661 agtattgacg gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc
    12721 aatcgaccaa tacgttgaaa aactaaaaac tgttactttc catgaaaaaa tcggtagtat
    12781 ctatgagaaa atgcagcggg tacaagtcat tgcccgtatc attggcgaac aagtaggact
    12841 atctgaggaa gaattgatgg atctgcaacg agctgctgaa atctataaat ttgatctagt
    12901 aaccaacatg gtaggggaat ttcctgaact acaagggatc atgggcgaaa aatatgctct
    12961 gctgaaaggc gagaaacctg ctgtagcaac agcgattcgt gagcattaca tgcctatatc
    13021 cagtgaagga gagctacctc agtctacagt aggttctgtt ttagcgattg cagataaatt
    13081 agatagcatt ttttctttct ttgcagttgg gatgattcca agcgggtcaa acgatcctta
    13141 tgccttacgt cgccaagcat acggtgtcgt tcggatcatc gaatcgaaaa aatgggcctt
    13201 tcctttgtct gttctgcaag aaacgattag tgatgttatc agtaaagata cggatcgttt
    13261 cggcattggt ttatcagaag ggcaacaaca ggtgatcgac tttatcaaag gccgtctgcg
    13321 ccaattattg acaacgaaga atattcgtca tgatgtcatc gaagctgttt tgaatgcaga
    13381 acaaaaagat ctgacaaaag tatttgcagc agcacaattg ttcaagcagc atcttgcaga
    13441 tgaagacttc aaaccatcga tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc
    13501 ggagcttgaa aaacaatcag aagtagaccc tgaactgttc gaaaatgaag cggagaaaga
    13561 actgcacaaa gcagtagagg caatgaaaaa aagctttgct gaccaaacga tcaacgaaaa
    13621 ttatgatcag cttgtaagcc ttcgcccact gatcgaaaat tattttgatc agacgatggt
    13681 catggcagaa gaaccagcag tacgtaagaa ccgattaacg caattaagtc aaattgctgc
    13741 catggctttg tcgctagcaa gtttagatca aatcatcaca aaataatttc taatatgtaa
    13801 ataaaccaga gaataggcac aaaa
//
LOCUS       DADJYG010000067        13650 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.068, whole genome shotgun sequence.
ACCESSION   DADJYG010000067 DADJYG010000000
VERSION     DADJYG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.068"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            146..484
                     /locus_tag="JZ382_001567"
     CDS             146..484
                     /locus_tag="JZ382_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427757.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(689..1264)
                     /locus_tag="JZ382_001568"
     CDS             complement(689..1264)
                     /locus_tag="JZ382_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAY2427758.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1873..2454)
                     /locus_tag="JZ382_001569"
     CDS             complement(1873..2454)
                     /locus_tag="JZ382_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2427759.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2637..3263)
                     /locus_tag="JZ382_001570"
     CDS             complement(2637..3263)
                     /locus_tag="JZ382_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY2427760.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LIESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3285..4616)
                     /locus_tag="JZ382_001571"
     CDS             complement(3285..4616)
                     /locus_tag="JZ382_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAY2427761.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4771..5172)
                     /locus_tag="JZ382_001572"
     CDS             complement(4771..5172)
                     /locus_tag="JZ382_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAY2427762.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5258..5464)
                     /locus_tag="JZ382_001573"
     CDS             complement(5258..5464)
                     /locus_tag="JZ382_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427763.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5559..7061)
                     /locus_tag="JZ382_001574"
     CDS             complement(5559..7061)
                     /locus_tag="JZ382_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAY2427764.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7079..7534)
                     /gene="argR"
                     /locus_tag="JZ382_001575"
     CDS             complement(7079..7534)
                     /gene="argR"
                     /locus_tag="JZ382_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAY2427765.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7632..8249)
                     /locus_tag="JZ382_001576"
     CDS             complement(7632..8249)
                     /locus_tag="JZ382_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427766.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8470..9843
                     /locus_tag="JZ382_001577"
     CDS             8470..9843
                     /locus_tag="JZ382_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY2427767.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            9918..11030
                     /locus_tag="JZ382_001578"
     CDS             9918..11030
                     /locus_tag="JZ382_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY2427768.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFEEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11102..12739)
                     /locus_tag="JZ382_001579"
     CDS             complement(11102..12739)
                     /locus_tag="JZ382_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY2427769.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLLDEAKESLENLIQKLN"
     gene            complement(12907..13515)
                     /locus_tag="JZ382_001580"
     CDS             complement(12907..13515)
                     /locus_tag="JZ382_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2427770.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3689 a         2867 c         2442 g         4652 t
ORIGIN      
        1 ttctttaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
       61 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      121 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      181 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      241 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      301 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      361 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      421 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      481 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      541 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      601 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      661 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      721 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      781 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      841 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      901 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
      961 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1021 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1081 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1141 gatttgacgg aaaaacttcc ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1201 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1261 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1321 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1381 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1441 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1501 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg
     1561 agcagctatt tttatttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     1621 ctccactaaa cggtgttcaa tcagctaacc ttcggtatta gctcaaaaaa cggcaaaata
     1681 tgaggaacta ttttgccgtt tgtttctcta atactcaggt caaagcgctg atttcacaac
     1741 ctctaataca cggtgttcca gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa
     1801 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
     1861 aacctctaat tattaaagaa ccttttgcga agttaacaat ctttctttga tcaattgaaa
     1921 attctgccta agtagtgata gatcctgtgt gactttcaac aataagatac ttccctggat
     1981 ttcagcaatc aacgctttgg cttgacggtc tgcttcttca tcggacagtt tttcttcaga
     2041 aagtgccagg atattttgtt tcgtatactt gacccattga ttgactaatt ctagtagcaa
     2101 cagacggtat tcttcatcca ctgtactcaa ctcaataatc aaattaccta caggacagcc
     2161 atgataagtg atctgtgtct catgatcagc gaaaatccag tccaacattt cttctaatgc
     2221 ttcttctggc tgttttccag catttaagat gtcttcgaac aattctgtct gccatacgtc
     2281 catatgcgca tggatcaccg ctagacaaac ttcttttttt gaagaaaagt aataataaaa
     2341 ttgacctttt cctgtatcag aagctgctag aatatctcgg attgacgttt catgatagcc
     2401 ttttttataa ataagatctt tggtcgcttc caagatcttt tcctttgttt tcaatgatat
     2461 tctccttttt catttctcat agtcctatat tatacagtaa attcatcatt ttttcttctt
     2521 aatcgtttaa aaaagactgt gccaaattat ctgacacagc cctcactata aaactgttct
     2581 catatagcta gatgttcatt gattcaagaa ctgcgaggac aaaagcaatt cctatcttat
     2641 gatagttgct ctaatacttc ctctgttgtc atcacttgat ctgcaataaa gccaaagttc
     2701 gtcaacgctg catccattgc ttcttggcca ggagcaccaa ttgcatctgt cgctaccaca
     2761 acattgaatc cactctctat caattcacgc atatgggaat cgctacacaa gtttccgttc
     2821 attccaccaa gaattactgt gtcgattcca ttttttctca attggagcgc caaatcattg
     2881 ctttctggtc caaagatttt atgaggagag ctgatgatcg tattttcatc caaataaggt
     2941 ttcaattctt cgataaaatc ggctcctgaa ccttcttcca ctgattcata ttgacttttt
     3001 ctagcaaaca tctgctgctc aagcatcata tgttctcctg caccgccaaa ttgccattta
     3061 tggtcatgtg ggtaaaaata atgaggagaa ataaagagtt tgtatccttt ttcttttgct
     3121 tgttttacca tttcaatcat attttcgatc gtattgtatt tttctaagat atcttttgtc
     3181 aaaccaaagc caccgcctgc tggtttcaag aactcgactt gcgggtcagt gagaactact
     3241 gctgtatttt tattattgaa catggtggat tctccattcg ttatttattt taatacatca
     3301 tttagagctt cactcattgt cggatgagaa tagatttgat ctctcaataa cgtataagga
     3361 agttttccat tcatagctag cgaaatcaag ttgatgactt catgagaatc ttccgcatag
     3421 atacttgccc ctagaatcaa gtcagtttca gggtcgacta aaactttgaa tacaccttta
     3481 ggatcttcta atacttgtgc tttagggaca ccgcttgtca tatacttgaa taatttgtaa
     3541 ttgacgcctt gtgctttggc ttgtttttct gttaaaccta cattggataa agtcggagtg
     3601 ataaatacac tgtaaggcac agctttccgg ttattcgttg ttcgtttatt ctcgcccttc
     3661 aactgatcca taatgattcg ataatcatct agagagatat aagtgaactg caatccgcct
     3721 ttgacgtctc caatcgccca aatgttgtct gcatctgtac gaaggtattc atccacagcg
     3781 attgctccgc gatctgttag tttaacatct gtattttcta atccaagtga tgctgtactt
     3841 ggttttcttc cagttgcagc caaaactttt gacgcttgga ttgttacttc ttggccatct
     3901 tctgtaaagg tgacggcagc tgttgattct tggtctgcta cttgatctac agatacacct
     3961 agatggaatt cgattcccgc atttgtcatg tcttctaaaa tcatttgcgc gatatcttca
     4021 tcttcacgtg gaagaaattc agaatgggca tcaagaacga caactttaga tccgtattca
     4081 ttgaacattg atgcaaattc caagccaata tatccagccc cgatgatcac taatttttcc
     4141 ggtaattgtt cctgatccat cgcttgcgta gaatcaatca agtagcgact tgtattcaat
     4201 cctttgattg gtaaaatgat tggttcagaa ccagtattga tgaaaatccg ttcgccttca
     4261 acaatcactt tttcaccatt attcgtcact tcgatttgat ggttagaaag aaaacgtgct
     4321 gtaccgtcca tcactgtccc agttgcctca tcgctgatca tatgataatt tttggcgcga
     4381 agcatgcccg tcaatttttc tttccgagca acagcctctg taaagtcgat tcctctttct
     4441 ccattgaaaa tcaatgattt agaaggaata cagccgatgt tgatacatgt tccgccatac
     4501 atacgtgttg acttttcaac gactaaaacc gattcacctt ttgaagctaa cgccttcgct
     4561 aaagtcttac cgccctttcc aaaaccgatc acaatatttt gatatttttc catcattttt
     4621 ccctcgcttc ttttttacct accgattggt acaaaaatat gataaccgat aattgtctat
     4681 tctccaaatc atctgcttta tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt
     4741 ttttagtctg gttcgatttc ctttattctt ctaaacaatc gttacttcta catcgatatt
     4801 tcctctagtt gctttggagt aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc
     4861 ttcttcttcg ctgatgccat cgatatgcac ttctaatact acaccgatct gatagccgcc
     4921 atcatctaaa ttataaagag agacacgtgc ttttactgtg gactctgctg tcattcctgc
     4981 ttctttgatc atatgttgta ccgccccatt gaaacaagaa gcataacctg ctgcaaataa
     5041 ttgttctgga tttgttcctt ctgcatggat tcctggagga gtgattttca tatgcatcga
     5101 gccatttggt gcctcgactt ctccattgcg cccgccatgg ttgatcattg ctgtttcgta
     5161 tgcttttttc attgtactgc tcctttcgat tgaccatttg ctacttggct ataattatag
     5221 tgtatgacaa gtaaggcgct ttctacaaaa attctgctta ttcgaatacc agaatgccgt
     5281 cttctactat ttttccaaaa aacaaaaaaa tactactcgt gaacaacagc gtcatatttg
     5341 ttccaaaata aatagcaatc ttttctacat aatcttttat atgattgatt ttaagataca
     5401 tatataccag aaagttgacc ccataaacca atactgttaa aaaaataacc atattcgtta
     5461 ccattttttc ctccttttgc tggtctccct accagcactc gatttaacca taccactttt
     5521 tttgccgtcc aagcaactag cctttcaaaa agactggttt attctgctgt taatgattca
     5581 agatcagaaa aatgtccgaa taattttgct aattttacta acgccaactt gttctttccg
     5641 aattcatcat tttctatctg taatactagt aacggctcat gtaagcttaa acggatcaaa
     5701 aaccagccgt ttccgtatgg ctcttctaga tcatatcgaa taccttcttc attttccggg
     5761 tcgaagttca tccctttttc ttcttccaac agtcgcttca tttcttctaa tatcttgttt
     5821 ccttgttcgc gtggttcatc gctgaacaat tttaatcgga tttcttgtac ttctacgggc
     5881 tgtttcaact ctttgatttg ttcatctaat gtgatacctt ttctttttaa ttctggcaaa
     5941 agcatcagga tctttgcgat gacatatgca ccatcatcca gaaaatagtt ttccttgaat
     6001 gcagcgtgtc cgcttgtttc gatggctagc tgtgtatctt tcccaagacg gttaagttct
     6061 aatgctttat tgattacatt tcgatagccg gaaatataac gaacttggat gcctcctttt
     6121 tcttcaatga atctttgtag atgagaagaa gtaggggaat tcgtcacgat tcttgttcct
     6181 ggatgctctt ttaatacgat tgtacttaat actgcgatca aattatttcg attgatcact
     6241 gttccagaag cactgacaac tgcagaacga tccacatctg tatcgaaaat gatcccaaga
     6301 tctgcttcat ttgctaaaac agcctgcttg atactttcca tcgcttcttt attatcagga
     6361 tttgggatat gattaggaaa tcggccatct ggttccaaaa attgggaacc tgttgaatcc
     6421 gcacctaagc tattcaatac tttttcagca aagaatccac cagcaccatt tccggcatca
     6481 acaatgattt tccaaccatt tagtggtttg ctttctagtg ttccaatccc ctttttaatt
     6541 tttgtgacta aatcttgcgc ataaggtgtc aaaaggtctg ctttttctac gcttccagtt
     6601 tgttcgcttt ttattttttg ctgctgtgat aggataaaag caatatcttc tttttctgct
     6661 cctccttctg aggtaaagaa ttttattcca ttataataat agggaagatg gctagcagtc
     6721 agcataaccg cagcatcgca cgagaactgt tcaaattgag tggacataaa catagcaggc
     6781 gtagtagcta attcgaaatc gatgacatgt atcccttgtt ctttcagact ttggatcaac
     6841 gcctctttca gtgctggtcc gcttatccta ctgtcatgac caatcccaat cgtccatttt
     6901 ttatttggat cggttgattt cttctttttc tttatccagt ttgctagtcc ccagccgatc
     6961 ttttttacca tgtctgtcgt caagtttact tcatatcctt caactggtaa tgcgattcct
     7021 cgaatatccg atccattttg cagcgctttc atatctttca tataaaagtc ctcctcattt
     7081 attccctcat tttttccagc ttttctgcaa atcgctgtgc ttcttcttct gagcggcaaa
     7141 taataatgaa tgtatctaca ccggccatcg ttccgacaac ttcagggtaa gccacttcat
     7201 ctagccaatt cgttaccaca tcggcaccat ttcgttcagt caatacgata accatgaact
     7261 gaacagactg gatacgcaaa acctcgcgtc taattgctgt acgcaaacgt tcttcattta
     7321 aagaaacaga tggctggtta aataatgtat agcgtactat tttgttttca ttttgacttt
     7381 ttactaactt catttctcga atgtctcgag aaatcgttgc ttgagtcaca tcggcgcctg
     7441 cttcatgcag ccgcgcaagc agctcttcct gtgtcccaat aggttgttct gtaatgatct
     7501 ggcggatcag tgcttgtcgc tgtttctttt tcatattaga cacctctcaa tgttgattat
     7561 agcaaaagat aagtaatatt tcttcatatt ttggtatttt ttacttttat tttttgtttt
     7621 cattcaggtt gctaaccttt ttctatttta acacgcaaaa tcgtcttcat tttttcaggt
     7681 gctgattttc ttggatcgct gatgtaaatc tctttgtgtt ctttagaagc tctttcatag
     7741 ccttcttttg cgatgaatgc ttctaatttc tcaaacgttg caggttcttc ggcatagggc
     7801 ccgacgtgta agatttgggc acaaagacct tcttccatct ttttgaatac tacttgttct
     7861 ttcaagtctt ccggaagttt gctagaacgc tgaatggctt cttgtgctat cgcttctgta
     7921 acaaagtcag gctgtcggat catcaattga taagaaaaat gttctttttt catcactggt
     7981 tcatgcaaat acttttcttg cagtccccat tgtccttcca gtggatagac agtatacatc
     8041 tgataatcag gaatcagcca atttttttta ggcgacatgc gtattgcata gctgactgca
     8101 tatagacagg ctactcttcg tttaaagtct tcctgattcg ggtcgccgat ccctctgatg
     8161 ctaaagaatg gttttttagg aacctctagt atttgcgggc tttttttcat actatagctc
     8221 ttgtctttac gccattcaat tttttccatt tttcttcctc cgcgaaattc catttagtta
     8281 tttattatca tacctctttt tgagtgactt ggctaatcgt gttgacacta tttttcagtt
     8341 tgtgctagaa taagtgaagt aaataaatcg tacagataac acgtttcggc gtgtcgagag
     8401 agaggcggtt tctattttta taatagaaat cactctcttt ttttacgaaa ttttttagag
     8461 gtgatgtaaa tgacaaaaca aaaacagctg cttttagtac ctgcagtaat tgcaacaggg
     8521 atgatgtctt ttgcgggcgt attgatcgag acggccatga atgtaacttt cccaactctt
     8581 atcagacagt ttgatttaac gactgcacaa gtccaatggg taacaacgat ttatcttttg
     8641 atgatttcga tcattgttcc gctgtctact tatttgaatc gcaattttag tctcaaaaaa
     8701 ctctttttag cttcaaatct gcttttcttg gcaggtataa tgatcgattt cttctctcct
     8761 accttcatgg tcctattgtt tggtcgattg ctccaaggtg cagcgacagg tatcgctctt
     8821 ccgttgatgt tccatattat cttgacattt gcccccctca atcgacgagg cgcgatgatg
     8881 ggtgtcggta ctttgacaac ggccattgct ccagcaattg gccccactta tggaggcatt
     8941 atgacttcac attttacatg gaatcatatc ttcctgtttc ttgtgcccgt tttgattctt
     9001 tctttgatta tcggctggtt tgctattccc gagatggcag tacaaaaaag tggttatttg
     9061 gattggataa gcgttttagg aatttgtctt ttgtttagtg gaagtctaac atttttgagt
     9121 acgttgggaa cagtttccag ctggattgct ttaatcatcg gcggtattgg ctggttcctt
     9181 ttttactatc gaagtaaaaa agtggagcat ccactgatcc gactgtctat tctttcaaat
     9241 aaaacattcc gcttgttttt gattggcttc ttagtttatc agtttttatt acttggtgta
     9301 tcttttgtct tgccgaattt ccttcagatc gttcaaggaa gtagtccttt tgtcgctgga
     9361 ttagcgatgc ttcccggcgc agcggttggt gctttgcttt ctcctttttc cgggaaaatg
     9421 ctggatcaat atggtccgaa gaagccaatt tttgctgggc tgattctttc tttgattggt
     9481 tggctagcat tattcctact cataggacac gcttcagtgg gtgtattgat tgcttgtcat
     9541 gtgttttata tgatcggtat cggattatct tacagcaata tgatgaccac cggaatgaat
     9601 gcactgaatg aagaactaca aggagacggg aacgcaatgt tcaatacctt gcagcagttt
     9661 tctggcgctg tggctacttc attagtagcg gtcattatca attacgtcca gcatcatact
     9721 tcccataact atgaagtgtc tactacatta ggatcaaaag cagcattagg cgtcttgttg
     9781 ctgttgcttc tcgtaagctt tgctcgattc gcctattatc tgttctttgc taaaaaagcg
     9841 tagattccat atacggcagg tgttgcatct gccgtttttt tgtgtgaaaa tggttgtttg
     9901 taaaaggagg cttttttatg gaaaaaattg ccattatcgg tggcgggatc attggtatga
     9961 ccctagccag ttacttagat acatcaaaat tcaatgttgc tttatttgat gaagggacag
    10021 gacaggcaac taaagctagc gctgggatta tctccccttg gttgtcgaaa cgcagaaaca
    10081 aacaatggta tcgtctggct aaagacggcg ctgctttttt ccctaaactt ataaaagatt
    10141 tccaacttga tcaaacgatt tacgcacaat cagggacact tttgcttcga cctgaaaaag
    10201 atttagctga attggctgtt cttgccgaga aacgaaaaga agaagcgcca gaaattggaa
    10261 acatcgaatg gctttcacct gaacagacat ctcaagcact tccgttattg aaaccagcag
    10321 tttcactgcg tatcagtggt ggtggtcgtc tggatggaaa agcatattta gaacatcttg
    10381 gtcagcgttt agcacaaaaa caaatggagc agatccaggc aaaagtcact ttgcataaga
    10441 agagcgattc ttggctcatc aagggaccgt ttgaagaata cacagctgat cgtgtaattt
    10501 tgacacctgg cccggcactt aagcctttat tacaagaatt aggctatgct gcagatatac
    10561 gtccgcaaaa agggcagctc ttagcatttc agacatctta taagatgagt caagactggc
    10621 ctgttgctat gcttgacgga gaagctgatt tcattccttt catggatgga aagatccttt
    10681 taggtgctac ccatgaaaac gatcaaggct gggatttggc tcctactagc gaagcttata
    10741 agggattgac taagagtaca gaacagttct tgactgatcc agatcagata aaaaactggc
    10801 catacgagtt ccgtgtaggt acccgtgctt atacgtcaga tttttcccca ttttttggcg
    10861 tactgccaga tgatccaagc ttactggctg ctagcggtct aggctcttct ggcttgacca
    10921 caggtcctta tatcggctat ctattagcca acttcttaaa tactggtcat tggtctacgg
    10981 aagattatca aaaggacatc tccacctata ttacgaaaaa tccatcatag aaaaacagct
    11041 ttttcaagta aggatcccga gcgatattgc gcatggatta cttcttgaaa aagctgttat
    11101 tttagttcaa tttctgaatc aaattctcca aactttcttt tgcttcatct agcaagagat
    11161 cttttcctat ctgagcttca atgaatcgta aacgaatatc aagtcgttgt ttcaccagat
    11221 ttttatagga cgtatcctca aaagaaacag gaaaatctgg tataggtaaa tagtggatgt
    11281 cttcaaatgg accaaacgac ttgaattctg ccgtttgttg cacgattctt tcaacgattg
    11341 acaatgtcgt ttcaggtttg atttttttat catcgaaata tccagtattc aggacatagc
    11401 aggctgtttg ctgtttcaaa aacaactctc gaaaagcttg atagtcttcc ctaagaggat
    11461 agacacggaa aggattagca aatggttcaa tgactagctc atctagctgt ctttttgtag
    11521 tgatattttc cgcgtctgat cgttttgttg ctaatgttgc accaaaagac gctgctaaaa
    11581 ctgggtcatt gatcttgata attggcggaa gcgaatcatc tttcatgatc caaaaaacag
    11641 catcgatctt ttcgctaaga tgatcgactc ggtcgggtgt cgcataacgt gatttgactg
    11701 tccgcccatt tccgtttcgt atatctcctg ttactaggac tttttctcct ttacttgtac
    11761 atgtcacgcc cacattctga caagtaagga aagaagaaat ggccggatca tcagacggat
    11821 agtcttgtgt tttatcaaag taagaaggtt ccaaggcagt agcaaaacca ttttttcgag
    11881 aaataacgaa agcatcgtca tgcaagatcg aaacagagtg accttccgta tgttttgcta
    11941 aggtaatcgt tgattttcca gaaccggaca acccaaatac agccatcgta taggtctggt
    12001 cattactagc atattgtttc atcccaccgt ggcaggcaat aaagccattg cgatttccca
    12061 atgcccaagc aagcgtcaaa gtcgcttttt ttaattctcc aaagtaacgt aatcctagca
    12121 cagccgctgt attatggaga gcatcaaaca aaatgagtcc atttggaaat gtttcatgtg
    12181 tccattcggg atcacaaaag atataaagat ctccctcatc aaaggaaaca gacttttgat
    12241 aagtctctaa gatcttcgaa ttcgctggct gaaaattcag caaataagaa tacaaattgt
    12301 tctcaaatcc ttctggaatc agcaaatgac tttgcacaga aaattcttca gataggccaa
    12361 cgattacttt tgtatggtaa aactggcgct tgcgactttg atacacagct tctcttaaaa
    12421 ttttctcgta tttgctttgt tccttgtcta agtcctcaaa gatccgccga gcacttgctg
    12481 ttcttcccac aatgcttcca tcattgaata ctagtgtcct aacatcagct ggtaaatcta
    12541 gttcttcagc atgtgagatt gttaaatctg tgatgatcgt tcctggactc ccttttgcta
    12601 gttgataagc tgtcttcctg tcaaaaactt catgaacatt attaccgtaa aatgtcgttt
    12661 ctacaatcga tcgaatactg gataacagtg gatttccact tgtgatttcg tttaattcga
    12721 gactctcttt tgtactcatg gctcctcctc attccctaaa caaaaaaacg ttttctttat
    12781 tatacattat tcgagttttt atttgtactg ctgcgctgtg ggaagcaaac gagttatttt
    12841 tcacacaaaa aaaccgagaa cttgaataaa caagtttctc ggtttcatca ttttcctttt
    12901 atcggcttag taccagccgt ttgctaacca gaatgtttta gcagcatccc atgaaccata
    12961 acgagatgtt acatattgtt ctgctacacg ttcttgattg gcagctgaat agtcgccgtt
    13021 taagtatgaa gcatctaatt gataacggcc gatgtaacgt ccgttagtag ctgtgtatga
    13081 accgcctgat tctttttgtg cgatccattc ttttgcagag cttgaagttg gcgctgttgc
    13141 agcagctggg gcagcaggag ctacttcaac tgtttcaaca ggtgcaacgt atgtatgttc
    13201 ttgcgttgct gtttcagttg tagatgctgt attttctgtt gttttgtttt ctgttgttgc
    13261 tgaagaatca tttgaagttg ggattgttaa ttgttcacca acataaatgc ggttgatgtt
    13321 tgcgatcgaa tttttttcag caattgcatc gatcattgag ttgtctcctg caaatttttg
    13381 tgagatctta gataatgaat cacctgattt tactgtataa acttcatcag cgtgagcatt
    13441 tgtagttcct acaaacattg cagtagcacc agcagcaaaa gttgtaccta aaacaatttt
    13501 cttaaatgaa ttcatattaa actccttttt ttctccgttt tttattcttt cgacgctatt
    13561 aactttatca tttagaaata tttcataggt aaaagaaaca tgacatttaa tgacaaaata
    13621 acgaattatt taatatttgt aacatttaaa
//
LOCUS       DADJYG010000068        13593 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.069, whole genome shotgun sequence.
ACCESSION   DADJYG010000068 DADJYG010000000
VERSION     DADJYG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.069"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(543..1091)
                     /gene="aac(6')"
                     /locus_tag="JZ382_001581"
     CDS             complement(543..1091)
                     /gene="aac(6')"
                     /locus_tag="JZ382_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAY2427771.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPDSQ"
     gene            1271..1596
                     /locus_tag="JZ382_001582"
                     /pseudo
     CDS             1271..1596
                     /locus_tag="JZ382_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
     gene            complement(1790..2857)
                     /gene="rlmN"
                     /locus_tag="JZ382_001583"
     CDS             complement(1790..2857)
                     /gene="rlmN"
                     /locus_tag="JZ382_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAY2427772.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSAHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTAAK"
     gene            complement(3091..5538)
                     /locus_tag="JZ382_001584"
     CDS             complement(3091..5538)
                     /locus_tag="JZ382_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY2427773.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLVAILLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQAQKQAA
                     KEERMKQRAEAKEARRLAAQEAAEKEAVEYEKKKAVNQQRKNTPDEWQEQENAEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGYANELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERIVQFVTDQQEAHYEEKMMPTDEVETAGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIDQEETSEPLPEDNDM"
     gene            complement(5727..7163)
                     /locus_tag="JZ382_001585"
     CDS             complement(5727..7163)
                     /locus_tag="JZ382_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2427774.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENGIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTNYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIVAGDEELLESAKPDFLGINYYQTATNAFNPLDGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRIHYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            complement(7785..8600)
                     /locus_tag="JZ382_001586"
     CDS             complement(7785..8600)
                     /locus_tag="JZ382_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2427775.1"
                     /translation="MKKISFLFALLVGLLLFAGCSNKKASADNWAAINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITSFEQMKNKRLGAQNGSSGYDVFTK
                     QPEVLKERVKNQDAVLYDSFNEALIDLKSGRIDGLLMDKVYADYYLKQRNELEDFNMT
                     KGSYESEDFAVGVRKSDDTLVKKINEAFDRLEKTGEFQKISNKWFGEDVTPRN"
     gene            complement(8619..9254)
                     /locus_tag="JZ382_001587"
     CDS             complement(8619..9254)
                     /locus_tag="JZ382_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY2427776.1"
                     /translation="MIELKKVNKAFGEKEIIKDLDLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNNYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            complement(9273..9914)
                     /locus_tag="JZ382_001588"
     CDS             complement(9273..9914)
                     /locus_tag="JZ382_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY2427777.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIILALGLSLHF
                     KPLRYFLQTYVWIMRGTPLLLQLIFVFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            10404..10889
                     /locus_tag="JZ382_001589"
     CDS             10404..10889
                     /locus_tag="JZ382_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAY2427778.1"
                     /translation="MNRATEQEAYELLRKLGISFQKVDHPAITSVKNIPFSLPGPQVK
                     NLLLKSRKGKKIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGIPAGTVTP
                     LALMHDTEKKIQVVIDSAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            complement(11320..13500)
                     /gene="acm"
                     /locus_tag="JZ382_001590"
     CDS             complement(11320..13500)
                     /gene="acm"
                     /locus_tag="JZ382_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAY2427779.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQDIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNIDSVEGWGQFEIQARNVTDTGEENTGPFTVMSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTEHIRWFLNINNDDTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPGATFDFSVNDNYIEITIPKNFVNLRK
                     IMVSYKTIIETPEQTSFENHSKAWFKEVNKPAVDGEGFNHTVKNISASGGVNGTVRGE
                     LKIFKYINGTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEVSIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRESTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENREVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWHYRFTNLPKYKDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPGKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRTNYKRTNYISKRAVP
                     KQITNKSKNILPKTSSKKSSFAVIIGLLIVTICTGIFLQKYKKV"
BASE COUNT         3777 a         2909 c         2260 g         4647 t
ORIGIN      
        1 tcgtgaggtt tcaaggaatt tttcagaaaa aatagctttg tacaaggttt tctattgcct
       61 tccagtaacc attaaataat ttttgttata ttgtctatta ttgccataca aagataaaaa
      121 agaataaaaa aaaggaactg tgacaaaagt cttttcacag ttccttatcc gaataaacgg
      181 tgttctcctg ccaactcctc ggtcacaagt cacaatagtc atcaaacatg agaagctgtt
      241 ttttgaaatt actccttgtg actcagagtg aaacggcaag ttcccgacct ctgggtaaac
      301 ggtgttcaaa agcttgcatc tccggcaata agcccaacca aatgaaaaat aggaggagct
      361 atttttgttt ggctgctctt attacttgat gcaggacagc tttttcacaa cctctccact
      421 aaacggtgtt caatcagctg accctcgata ttcgctcaac aaacggcaaa atatgaagaa
      481 ctatttttcc gtttgttgag ctaatactca ggtcaggacg ctgatttcac aacctctttt
      541 tattattgag aatctggtcg aggaataatc gtttttgcca tccaaatatc cggtttgtcc
      601 caaccatttg catttggtaa gacacctacg attttataac ctaatttttc atagaactca
      661 tacggatgtt cacgaaggtt ctggatggaa gccactttat caaacgtatg ctcatacagg
      721 tccgtttgac ttaacgttgt tccatggtct aaatcatccg tacctaaata aatcgttatt
      781 cctcctctgg aagctacttc tttttctaag taattgacta atcgagtacc tatttggttc
      841 tttcgtcgag agctttctac aactaatgga tgcaattccc aacctgtgat accgtattga
      901 gggattgcac caataaatcc tactaactca tcttggtcta ccgctgctac cgcgattcgt
      961 tctggattca tcatttcttc tacttcttct gccgagctgt ctccatattc ttccggccaa
     1021 gtcagtctca gtaaatcaga aagctggtct ttcaatactg gattattacg gtcaaattca
     1081 ctgattatca tacgtcttcc cccttgttct tgcttattct aacagtcctt ttgaaaaaac
     1141 gaaatgttct cgctcaaatt ctttagcagt caattatttc tctagcaaac taatcggctg
     1201 ccctgcttat aaagcaaaaa aaaactccag aaatactatt tctgctaagg tacacatatt
     1261 ctattgcagt acgacctcta aaaaaaatgg agggatacct tcacggagca tctgtcagtt
     1321 atggtgtact gaccttattg atgatggatc agcagttcga tttgtttgat aaaatctatg
     1381 tgtttatgaa aaaaacaact tccagtatcg ttaaccgaca tgaatctcaa tatcacgacc
     1441 gatttagaag caatcttaga taaagccatg accacaaatg atatcaaaca cgtgccttat
     1501 gaaatctccc gagaaaaatt taaacaggcc attcttgacg tagaaactta tcatgctaca
     1561 caagaaagcc agcaaggata taagaaaatc agttaaataa ttgctaaaac ccccatcaag
     1621 caggctgtca tcaaattgac gacatttctt tgcttgatga gggcttttct tgtcaaaaaa
     1681 cgctttataa ctttcgctat agaatctttt cacttattta agttggacag ttccttaatt
     1741 aataaaactg ctccttttat ggtaattgaa aaaacaaagc aggctacgat tatttggcag
     1801 ctgttttttt catttgcttg cttcgcaatt gtccgcaagc tgcatcaata tctgtaccat
     1861 gttctttacg tatcacacag tttacgccgt ttttcttcaa tacatcataa aatctcaata
     1921 catcagcttt tggactgcga gaatattgat catgttcact gactggatta tatggaatca
     1981 agttgacata tgctaacttt ttcttatctt ttaatagatc tgctaattgt tgcgcatgct
     2041 ctggtctatc atttacttga ttcaacatga tatattcaaa cgtgattcga cggtttgttt
     2101 tctctaaata ctcatccaca gcttccatca atttctcaat tgggaaactt cgattgatcc
     2161 gcataatcga tgttcgcacc tcattattcg gcgcgtgcaa ggaaatagcc agatttactt
     2221 gcaagccgtt attagcgaat tccttgattt tgtgtgctaa gccactagta gaaactgtaa
     2281 tatgtcgcgc accaatcgcc aatcctttgg catcattaat aatatgcaaa aagtccatta
     2341 cattgtcgta attatcaaat ggctcaccga ttcccattac gacgacatgg gaaacacgtt
     2401 cgccaagatt gcgctcatca aaataatgtt gaaccatcat gatctgcgcg acgatttctc
     2461 cagcggtcaa atcacggttc tttttaagca acccgcttgc acaaaatgta cagccgatat
     2521 tacatcctac ttgtgtggtc acgcaaacgg acaagccgta ttcttgtctc attagtacgg
     2581 tctcgatcat atttttatca ggaagttcaa acaaatattt tactgtaccg tccgaagctt
     2641 cttggacgac catttgtttc agaggattga tgataaaatg tgccgataat ttttctatca
     2701 attgttttga tagattcgtc atttctgtga agttacttac acgtttttga tacaaccatt
     2761 cccaaacttg ggcagctcgg aattttttct caccgttttc tataaaccaa ttgattaatt
     2821 cttcattcgt caagccatag atggattgtt tttccaaagt attttccctc tttttctcaa
     2881 agtttcgcta catactatac atgattcgtt tagttaatgc aatacttcac aaataaatcg
     2941 aatagaactt tcatctcata aaaatcaaaa aagacaaaac atcgtaccgt attagaaaag
     3001 atgggaaaaa agccattttt tctcttggta tcatcatgtc ttgtcttttt tatatagtta
     3061 ttcgtttttc aaccgttcat tgaacaagtt ttacatatca ttatcttccg gcagtggttc
     3121 tgatgtttcc tcttgatcaa tgaatacttt ccgaggctta cttccttctg atgggccaac
     3181 aactccatga gcttccagtt catctacgag tcgagcggct cggttgtaac cgattcgaaa
     3241 tcttctttgg agaagggaga tacttgctgt ttgcatttca acaacaagtg cttttgcttc
     3301 ctcaaacagc tcatcttgtg gctgttctgg ggcacctgcc gtttcaactt catctgtcgg
     3361 catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa cgatccgctc
     3421 cacttcatgg tcagagatga acgctccttg cacacggata ggtttattct cacccattgg
     3481 caagaacagc atatctcctc gccctaagag cttttctgct ccattagagt cgatgatcgt
     3541 ccgagaatct gtaccacttg atacagcaaa tgccattcta gaaggtacat ttgctttgat
     3601 gatccctgtg ataacatcca cacttggacg ttgagtagct aggatcatgt ggatgcccgc
     3661 tgcccgagcc atttgcgcta aacgaataat cgcatcttcg acttcgttgc tagctaccat
     3721 catcaagtca gctaattcat ctacgattac tacgatgaat ggtaaggttg gatgtttcgt
     3781 tccgttttct aagtttttct gctgaacaaa ttcattgtaa ccagaaatat ttcttacacc
     3841 agttgccgca aatttttcat atcgttcttc catttcttgt actacttttt gtaaggcttg
     3901 tgccgctttt ctagggttcg ttacgaccgg cgtcaacaga tgaggtatcc cgttgtacat
     3961 atttaattca accattttcg gatcgatcat catcagtttt acttcatggg gcttagcacg
     4021 catcaatata cttgtaatga tcccattgat tgctactgac ttcccactac cagtcgaacc
     4081 agctacaagt aagtgaggca ttttcgaaag atcagccgtt tgtactcgtc cagaaacatc
     4141 acgtcctaag ggtacttcca gtaatttatc tggatggttt ggctgagctt cgatgatttc
     4201 tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca gtgatttacc
     4261 tgggatcggc gcttccatcc gaacatcttt cgctgctaaa gcaagtgcga tatcatctgt
     4321 taagttcacg attttactta cttttacccc tacagctggt tggacttcga attttgtgac
     4381 ggatgggcct aaactagctt tgacgacctt agcatccaca ccgaaacttt ggaaagtttg
     4441 ttccaataca ccgatatttt tttcaatttt tttatactca tcactttgat ccacagttgg
     4501 tatagaatca agcaagtcaa ctgtcggcaa ttcataatct tgattttcct gttctgcttc
     4561 gatctcaaat tctaatgagg taccgtcatc ctctggcaac tcattagcat aaccttctgc
     4621 tgtattggtc tgttttggtg tatctgggac tggtttttct agattcgcag gatggatatt
     4681 ttcttgaaaa ctgtcaattg gaacaaaact taactgctca ggttcggcat tttcttgttc
     4741 ctgccattca tcaggagtat ttttcctttg ctggttcaca gcttttttct tctcatattc
     4801 tactgcttct ttttctgctg cttcttgtgc cgctaagcgt ctcgcttctt tcgcttctgc
     4861 acgctgtttc attctttctt cctttgccgc ttgcttttga gcttgcttgg caggatcacc
     4921 ttccaataat tcctgtaccc gttctccaat tgattggata ccattcagca gctgatagcc
     4981 ttccaaatta ctgaacaaga atgctccacc cgccagtaat agaatggcta ctaaataact
     5041 tccaggctgt gcaatcaaga aataagtgaa atgataaaga attgctccga tcatccctcc
     5101 gccaacgtta ttatctactt gactttgttt tatatcgcta gccaataagt cccatgtcgt
     5161 ccccatgata tttggttccc cagtatggag tttgccgaat aaatgggcat gtagcaataa
     5221 taagataccg aaataaaaga gtataccgcc aaaccagcgt cggtttttgc caattgaaaa
     5281 ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac agaggatttg
     5341 gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta aaaaacctaa
     5401 ttttaaaaag ccgaatacag caaatagaat caaggcaaat ccaacaccaa tcgtcagcat
     5461 ttgttcttgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt tcttagtagg
     5521 ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat acaaaaaaaa
     5581 gcaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt tcagcttatt
     5641 ttacagtttt tcttaatttc taaatgattt tatcaaaaaa aggcacggcc atatccagcc
     5701 gtaccttctt gttttaagca tgagaactaa aggttctctc cattttcttc aatcagtttt
     5761 tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg tgtttcatct
     5821 tgatcgacat atacaaatcc atatcgcttt tgataaccgt tcagccaact cagcaaatct
     5881 gtataagacc atgtacaata gcctaatact tctgcgccat ccgaaattgc ttctttgatt
     5941 gctttgacat ggctttgtaa gtaatggatt cgatagtcgt catggatttg tttgtcttct
     6001 gtcaacttgt catattctcc taagccattt tccgtgatca gtaccggcat gcggtaacgg
     6061 cttgtgatac gacgcaatcc gatccttaag ccttctgggt cgatctccca gtcccaattt
     6121 gttcgttcga caaatggatt ctcaactttt ttatacattc caggaacccc tgtttcttct
     6181 gatgaacctt ttttcccagt cgtattcatt tttcctgctc caactccatc taatggattg
     6241 aatgcattcg tcgctgtttg atagtaattg atcccaagga aatctggctt ggctgattct
     6301 agcaattctt catcgccagc tacaatcgtt ggcgcccatc ctttttcttc taagaatctc
     6361 atagcagcaa taggatattt accccacatg tagacatcca tccaataatt ggccatcaga
     6421 tcttctgcat tttcggctgc cagaacattt tcaggattac tgtcgattgg ataatttggc
     6481 gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcatggaa tttgttgatg
     6541 acagatgcat ttgctagatt tgcgatatgg ttgacttcat acattcgttt tggatccgtc
     6601 acgcctggcg gatgtgctgc taataaatag cctaatgaag taaagatatt ttgttcattc
     6661 aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc tgcataatta
     6721 gtgaaatctt caatgatttg acgtgattcc caaccattat attcatcttg caatgcttgt
     6781 ggaagatccc aatgatacaa agtcaacata ggctcaatac cgttttcgat caattcgtcg
     6841 atcaatctct cataaaaagc gagccctgct tgattgactt ctcctcttcc attaggaaaa
     6901 atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt cattaaagca
     6961 atatcttctt tataacgatg atagtggtct actgccagat caccgtttgt tcctttaaat
     7021 gtctttcctg gaatccgaac aaatcgatcc cagactgatt cgcctttccc atcttcttgc
     7081 caagctcctt ctacttgata cgctgcagat gcagagcccc acagaaaatc ttttggaaat
     7141 gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca atcgttcctc
     7201 ttttagaata acacgaaacc ccttccacaa caaaataaaa ttatgtaaag gaagttgtga
     7261 aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt
     7321 gtgaaatttc ggcttatttt tcgaagaagg tacttctgga acaccgtgta taagaggttg
     7381 tgagatcagc gttctgatct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
     7441 ttgccgtttg ttgagctaat accgagggtc agctgattga acaccgttta aggaagaggt
     7501 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtggaaatg gctttctaca
     7561 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttaaggaaga
     7621 ggttgtgagc ctgccgttca gctccgagtc acaaggagca atttcaaaaa acagtttctc
     7681 atgtttgttg aatatcgtga cttgtgaccg aagagttggc aggagaacac tgtttattcg
     7741 gaaaaaggga ctgggacacg tcttttgtca cagtccccta tttgttaatt tctcggcgtg
     7801 acatcttcac caaaccattt attggagatt ttttggaatt cccctgtttt ttctaatcga
     7861 tcaaatgctt catttatttt cttcactaac gtatcatctg acttacgcac accaaccgca
     7921 aaatcctcac tttcgtaaga gccttttgtc atgttgaagt cttctagttc gtttctttgt
     7981 ttcaaataat agtccgcata tactttatcc atcagcaaac catcaatccg tccgcttttc
     8041 agatcaatca gtgcttcatt aaaactatcg tacaaaacag catcttggtt tttgactctc
     8101 tcttttaaca cttctggttg tttcgtaaat acatcataac cagatgaccc attttgcgca
     8161 cctaatctct tatttttcat ttgctcaaag cttgtgattc ctgattcttt aggtgtgatc
     8221 agcacttgat cattttccat ataaggtttt gtaaactgca ctttcttttc tcttgccggt
     8281 gtcttggaat agccattcca gatcaagtcg attgttccat tgttcagttc aaattccttc
     8341 atcgaccagt caataggctg aaagtctacc gtaatcccat attgttcaaa gactgctttg
     8401 gctaaatcaa tatcgaatcc tgtaagcttc ccatctttat ctctaaatcc cataggaaca
     8461 aatgtatcgt ccaaacctat aacgattttt ttctcttcgt tgatagccgc ccaattatct
     8521 gctgaagcct ttttattcga acagcctgca aaaagaagaa gacctacgag caaagcgaat
     8581 agaaaactga tttttttcat ctctatcttc ttcctttctt aatgaattgg ggaaactttt
     8641 aatagttgat caccgacatt ttctgcaaat acgagatcat gggtgacaat gatttgggtt
     8701 accccgtctt ctttcaatga taggatcaaa cgctcgactt gctgacgcaa ctcagggtct
     8761 aaagcactcg ttggttcatc ataagccaat acccgtggat tcatggccaa tgctctagca
     8821 atcgctaccc gttgtttttg tccgccagaa agctgataag gatagttatt caataaatcg
     8881 ctaatcccta atgaagccgc taattcttct gcttttttgg tgtattcttc ttttgtctgt
     8941 ttcaatacca ttttcggtgc taaggtaatg ttttcaaaaa tattcagatg tggaaatagc
     9001 tggaagtctt ggaatacgac tcctacaact tgttctttca ttttaagttc gaatggattg
     9061 aacggtacac cgtctaaata aaattctccg ctgtcagagc tttctaagcc agccaagata
     9121 cgtaaaagtg tggttttacc gccaccggaa ggacctacga tagctaatgt tttcccatct
     9181 ggaatgatta gatccaaatc tttgattatt tctttttcac caaatgcttt gttcactttc
     9241 ttcaattcaa tcatacttct tcctcctatc agttagcggt aataatttaa ttttccttca
     9301 acttgtttca ttacgattgt tagaatacca gtcaatgtaa gatagatgat tgccactcct
     9361 agcaaaggca gtaaagtcgc gtcgcgttcc atcgcgattt tcccggcacg cattacatca
     9421 cccaaaccta atatatagat caaggaagaa tctttgacca agttgataac ttcattacct
     9481 acagaaggta aaacaatctt tgtcacttgc gggatgacga ttttcgttac cgtttggaag
     9541 ggacttaatt gcaaaacttg cgctgcttca tattgacctt gaggaatcga ttgtatccct
     9601 ccacggaaga tctctgcaaa ataagcggca tagttcaaga taaaagcaat gagcgctgct
     9661 tcgaatcgat caaaaacgat cccaatcgtc ggcaaaccat aaaaaacaaa aatcagctgt
     9721 aacaatagtg gcgttccccg catgatccat acatatgttt gaagaaagta gcgtagcggc
     9781 ttaaaatgca aggacaatcc tagtgctaaa ataatcccta atggcaacga gccaatcaag
     9841 gtaaataaaa ataatttcaa tgttgtgagt gctcctacta ataattgcgg cataacttca
     9901 attaaatact ccatcttctt ttcctccttt aaaataaaaa aatcctcttg gcaaatgcca
     9961 agaggacgta ttatacgtgg ttccacctct gtttattttt tccttgcaga aaaaacctca
    10021 cacagtcgtt gcaaatcaga tgaaaacaaa acgtcctttg atccgaagat caaaggacgt
    10081 taatcacgtg gttccacctt tttttaccgg ctcctcacgg aaaagccagc cttacctggt
    10141 catctagcaa cgaccgcagc tgtaacgggc tccccggatt ttcctactat acgcttcaga
    10201 aaatcgactc caagatgtgt ttcacggtta actgttgtct ctttccacca accagagact
    10261 ctctaaaaac acctaactgc tactcttctt ttcgatgtca tttgttttaa ctttacgtgt
    10321 aatttacatg attatttttc atttgtcaac tcttgtcaac tcttttttat gatacactaa
    10381 taaaaaaagg agggtgatgc gcgatgaatc gtgcaaccga acaagaagct tatgaactac
    10441 taagaaaact aggtatctct tttcaaaaag ttgatcatcc tgcgattacg tcagtcaaaa
    10501 atatcccttt ttccctacct ggtccgcaag taaaaaatct attattgaaa tcaagaaaag
    10561 gaaaaaaaat ctatcttgtc atcttgcctg atgaaaagca tgccgattta aaacaactag
    10621 ctgaacaatt aaaagaaaag cgcttatctt ttgtttctga agaacagctt cctgaccttc
    10681 taggaattcc agcaggtaca gtgactccat tagcattgat gcacgacaca gagaaaaaaa
    10741 tccaagtcgt gatcgattct gctattgatc gtcaagatac cgtaggcttt catccaaata
    10801 tcaataccac tacgttgatc atggattttt ccgactttga aaagatactt gtttacttag
    10861 aacatctgcc agtttatgaa aatttgtaat gatagagtag ttttcttaat tcccaattca
    10921 agcgtctttt tctttattta gcacaaaata tatcagaaat tcttttagcc aatcaaacaa
    10981 tgtaacaact agtatgaacg ctgaaaacta gagtattctt ccatagttag ccaaatccat
    11041 atttattgat tctacgaaaa gaggctagga cagaagcgtt taactccaag agataagaag
    11101 aaatttacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagtgg
    11161 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    11221 tcccatgctc tttttgatta taaagcggaa ttttatcctt ttgctttcag tcatatttaa
    11281 caaaaaatat tatattctga ataacaaaaa ttattctatc taaacttttt tatatttttg
    11341 taagaatatc cctgtacaga tagttactat caataaacca atgattacag caaaactaga
    11401 ttttttacta cttgttttag gaagaatgtt ttttgattta tttgttatct gtttaggtac
    11461 agctctctta ctaatataat tggttctttt ataattggtt ctttgttcgc tagattctcc
    11521 tgtatgagca aatatttgca atttttgaga gaatagattt ctaattttct ctctctctgt
    11581 cttctctgga aaatttttcc acggtgttat atggctacct ggtatagata tatttgtttt
    11641 gcccggtgta taactgtttt tgatgttata cccgtcaatt gtcgtactgt attctggtac
    11701 attgtcttcc gttacagtat agatgatttc ttgcccgtct ttgtattttg gcagatttgt
    11761 gaaacgatag tgccaatcat ctttttcact tacttcttta tgttggattg gttgaccgtc
    11821 ggctaataga ttcactgtga tcgttgtcgg tcgtttaccg tcttgattct cattgtcttc
    11881 ccatgttttt tgtcctgaga ccgaaatgac ttctctattc tcatttgtaa cgactagccc
    11941 gttttcaatt ttttgataac ctgaagggac gtaatcattt ccgttttggt ctacttcttg
    12001 cactttaaac gtatattcgt ttccactatc atcatactgt tggatatctt cccaagtcac
    12061 tgaagttatt ccattatcta atcgctttgt ttctgcatca ggaactggtt cccaattgtt
    12121 tgtactttct ctgaataatt taaaataaat acttggtctg ggagtggttc caccgttcca
    12181 aattttagtt gctgtgatgt tcgtaacctt tttcttgtta taaataggta aactgactcc
    12241 ctctgtatca ttttcattta tggagaattt gagttcattt gaacttagtg gatcaaaatc
    12301 aatccaattg ggtgcttctt tttcttttac aacataatct cctacttgca gacctttgat
    12361 actaacttct ccctgttcat tcgaagtcaa caggatacta gactgtcctt gaataggctg
    12421 ttcatctgct cgtcttaatt cgaaagtcac atttgggata ccaatctctg tgccattgat
    12481 atatttgaaa atcttcaatt cgcctctaac tgtaccgttg actccaccag aagctgaaat
    12541 atttttaacc gtatgattga aaccttctcc atcaacagca ggtttattaa cttctttaaa
    12601 ccaagctttc gaatgattct caaaacttgt ttgttctggg gtttctataa tcgtcttata
    12661 actcaccatt attttcctaa gattgacaaa gttttttggt atagttattt caatgtaatt
    12721 atcatttact gaaaaatcaa atgtagcacc ggggaaatct tgtaaaaatt gttggattcc
    12781 ctcttctcct ctgtaaactt tctgttcacc taagtgtatt tgattaattt caaacgtact
    12841 tggatctaat ctttgtccac tttggatttc atcactgatt ttaactggtt gttcaacgta
    12901 agtgtcgtca ttgttaatat tcaaaaacca tcgaatgtgc tcagtatctt caggaagcat
    12961 gtctcctgtt ttataataaa aaacgctaga agaagaacca gaagctggct ttgtcacatt
    13021 tacagtagcc gttttgtcac cactcatcac ggtgaatggc cccgtatttt cttcaccagt
    13081 atcggttacg tttctggcct ggatctcaaa ttgcccccac ccttctactg agtctatgtt
    13141 tttaatattt tcattaaata caacctgtgc agaatctttt ctgaccgtta aagttccaac
    13201 atttttattt tcaatcatta atgggatttc tttctggaat ccttcaccgc gaatatttcc
    13261 ttcacttggc caattgatcc aaagataatc acctggctgg atatcttgtt ttttttcgtc
    13321 aaacgagaat tttactttta tgtttcctcc atctgtaata ttcgttgggt ccacggtcaa
    13381 cgaagtgacg ttactgctga tatctctgcc tgcatccgca tagacatgaa caggaatcag
    13441 tgatgttata ttagtaataa taaataacat tgataaaata atatttctac actttttcat
    13501 tttttctatc tcccctattc taactaaaaa taaaacctac catatcatct ctcgaaaatc
    13561 ctttcttcaa ttcctcccct actcatttcc aat
//
LOCUS       DADJYG010000069        13329 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.070, whole genome shotgun sequence.
ACCESSION   DADJYG010000069 DADJYG010000000
VERSION     DADJYG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.070"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            187..954
                     /locus_tag="JZ382_001591"
     CDS             187..954
                     /locus_tag="JZ382_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427780.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            944..2986
                     /locus_tag="JZ382_001592"
     CDS             944..2986
                     /locus_tag="JZ382_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427781.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3267..3416
                     /locus_tag="JZ382_001593"
     CDS             3267..3416
                     /locus_tag="JZ382_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAY2427782.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3427..4941
                     /gene="dltA"
                     /locus_tag="JZ382_001594"
     CDS             3427..4941
                     /gene="dltA"
                     /locus_tag="JZ382_001594"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAY2427783.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4938..6143
                     /gene="dltB"
                     /locus_tag="JZ382_001595"
     CDS             4938..6143
                     /gene="dltB"
                     /locus_tag="JZ382_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAY2427784.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6188..6421
                     /gene="dltC"
                     /locus_tag="JZ382_001596"
     CDS             6188..6421
                     /gene="dltC"
                     /locus_tag="JZ382_001596"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAY2427785.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            6424..7692
                     /gene="dltD"
                     /locus_tag="JZ382_001597"
     CDS             6424..7692
                     /gene="dltD"
                     /locus_tag="JZ382_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAY2427786.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7833..8915
                     /locus_tag="JZ382_001598"
     CDS             7833..8915
                     /locus_tag="JZ382_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAY2427787.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8915..9718
                     /locus_tag="JZ382_001599"
     CDS             8915..9718
                     /locus_tag="JZ382_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAY2427788.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9788..10528
                     /locus_tag="JZ382_001600"
     CDS             9788..10528
                     /locus_tag="JZ382_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAY2427789.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10539..11543
                     /locus_tag="JZ382_001601"
     CDS             10539..11543
                     /locus_tag="JZ382_001601"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAY2427790.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11588..13285)
                     /locus_tag="JZ382_001602"
     CDS             complement(11588..13285)
                     /locus_tag="JZ382_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427791.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4282 a         2186 c         2699 g         4162 t
ORIGIN      
        1 gaaaaaaggt gaaaacttgt agctggtgga tttgtttttt gcatatttaa ttttgctgaa
       61 taaaataatt gaataaaatt ttttattcag tccaagtaaa agtgacaaaa acataagagc
      121 aaattattcg tagatatgct actatgcaaa taaatagaaa tgaaaaatga aagaggagga
      181 gaagaattgg aaaagatgat cgaagtgaaa catctaagta aaagctacgg cagcaaactt
      241 tctcccgtca aagccttaga taatatctca ttcacagtag aagaaggcga gtttgttggg
      301 attatgggcc caagtggtgc aggaaagaca acgctgatga atatcttgtc cacgatcaat
      361 ttgcctacga tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt
      421 gagctcagtg actttcgtcg aaaaaaacta ggattcattt ttcaagagtt caatttgatc
      481 gataccctaa atgcaaaaga caatatccta ttgcccctag cagttgaacg gatgactaaa
      541 gaggaaatgg aaaagagagt aagacatgta gctcaattgt tgaatataga agaactgctc
      601 aaacggtatc ccgatgaact ttctgtcgga cagagacaac gaattgcagc tgctagagca
      661 cttgttgttc agccacaagt gatttttgca gatgaaccaa ctggctcttt ggactcgaag
      721 tcggctacag aattgttgaa ctacttgaag accatgaatc aaaaagaaaa agcaacgata
      781 ctgttagtca cccatgatcc atatacagca agctactgcg atagaatttt atttattaaa
      841 gatggtgtga ttttttctga agttgtcaga agagggacaa gaagagagtt ctttgagaaa
      901 gtaatagaca tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt ataaactagc
      961 cgttaaaagt ttttttcggc agtcacgagg ctatcttgtt tattttttca gtttgacttt
     1021 atccgtaatg atctattatt ccttcagcgc aatgacctac gatcaatctt tagtcagaag
     1081 agcaagtcaa gatacttcga tagatggtgg attgagtttt ggtggattta tcattactgt
     1141 tgttttgctt tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga
     1201 aataggcatc tatcagcttt ttggtatgaa taaactgcag atatcaggta tatatgtgtt
     1261 agaaatcatg ataattggat tgttcgcctg tatttctggg atattactcg gtattatttt
     1321 ttccaaactc ttttcaatga ttttggttcg aatgatggat atggatctta ctagtccatt
     1381 ttttatctcc attccatcag tagtagatac tttatttgct tttttcatca tccttttagc
     1441 agtatctgta tattcaatat ggaaaatatg gcgctatcca atgattcgaa gttttggaga
     1501 aaaagaacaa gcagatagtt caacaatgcg cttcaggaca aggcaccgat tactcgcagt
     1561 catcgggtta ctacttttaa cgagcggata ttttggagct agtcatttta gggaaatcac
     1621 ttcatggatg atcagttatc attatcttga tacattagtt ttcttttttc cttttttaat
     1681 ttttttcgtt tgtgtggtcg gtacttattt gtttttctgt tatacatttc ggttattttt
     1741 gatcatattg agtaaatgga agatttatta ctatggtatc aattttttga taatgggtaa
     1801 cacgcaagtt catttgctca aaagttggcg tacaaattct ctgattacag tcattctcgg
     1861 aatttcttta atgacaattg gcggcacggc tagtatctct actattttat cctaccagga
     1921 gaaagtttcc tccccgatgg actatcagtt tgatgttgag acagagaaaa aaatcaagcc
     1981 gattctagca gaggaagggc agaagataac taaagaaatc accttgcatt ataaagtcat
     2041 tgggagtatc tatagtcaaa atctcgaaga acctatgttt caaactgcta atctgatcac
     2101 ggaaacacaa tatcaagcct tcagaaaagt taatccagat ttgccgaaca tttctttgaa
     2161 atcacctggt catactgttt tgcttgatca aatgcaaact ctttttcgga atatttcatt
     2221 atatggatct agtattggac tgccagataa tcaagaattg aagattcaac aaatgcttcc
     2281 aagtgtatta ggtgacgaaa caatggtata tctaagtcct gtactgattg tagatgatac
     2341 cgtttttgaa aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg
     2401 aaataatgaa aaaatagatg aacgactgag caatgacctg tcgataaagt ggctcaatgc
     2461 tgtttattat tcttatgata tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa
     2521 acttccggat gaccaaatga aggaagcaca atttagccaa gaaagctctc gtttgaattt
     2581 ctcttccagc tattcgaaat tgcgtgcagt taatcggcga atcggtatca atatttttgt
     2641 cacactattt gtcgggatga tcgtgattat tgctactggt agtattttga cagtccggca
     2701 attagcagaa gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc tggggattcc
     2761 acaaagaagg atcagacgga tgatcttcga gcaaaacgca cttaccttct ttccacccat
     2821 gatattaggg atcactcacg cagtatttgc aatcaatgtc ttcactcagt atataaaaac
     2881 tgccgattac tggttggctt atcttgtgag tggcttattg atccttatct atcttttgct
     2941 ttatgtcatt acgagtcagt tatattgtcg gattatcgag gaatgatata cggaataaga
     3001 caagataata aatagaacta aattttgaat accatttatt tggaataaga ggttgtgaca
     3061 cgatttttgt cacaacctct ttatttttta tattttttaa aagtacgaga aattataaag
     3121 aaaaaagcag tgatttgatg tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa
     3181 aaaaacggtt tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt
     3241 gtgaggttat atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt
     3301 attggggaaa attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt
     3361 atctgtatca ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg
     3421 aagagtatgg aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct
     3481 gatcgagttg cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca
     3541 gacgcacttg cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt
     3601 ggcaatttgg agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc
     3661 tatttgccga ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct
     3721 aaaccatcta tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc
     3781 ataacaaaaa aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat
     3841 agcgtaaaag gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca
     3901 aaaggcgtac aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac
     3961 tttgggatca agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg
     4021 gtattttcta tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca
     4081 gttgttcaag actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg
     4141 gtttctactc catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat
     4201 gtgccggaat tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa
     4261 agtctacttg atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca
     4321 actgtagcta tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta
     4381 ccgattggct acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt
     4441 tcaaaaggag aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg
     4501 aacaatcccg aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat
     4561 acaggggatg caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat
     4621 cagcaggtca aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc
     4681 caagacaacc gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag
     4741 cagctggttg cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact
     4801 aaatctatca aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc
     4861 cattatgtcg atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca
     4921 gctgaggtga atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt
     4981 tttttctttt agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa
     5041 gattaccagt ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta
     5101 attattggca acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat
     5161 ggggctattt tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta
     5221 ttttagcgat aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag
     5281 catcgttact tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca
     5341 tggaaataag agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc
     5401 tgttctttcc aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc
     5461 agctccatcc gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata
     5521 tttttgtcgg ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc
     5581 ctgtagtggg acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata
     5641 tgtacgtgta cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg
     5701 gtacaagcta catgatggga tatcagacac caatcaattt caataaacct tttttatcat
     5761 ggaatatcaa agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt
     5821 atgtatatat gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg
     5881 taatgtcaaa tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga
     5941 cgtggtacta catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat
     6001 ggatccgatt taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct
     6061 tagccgtatt tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt
     6121 tagataaact tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa
     6181 aaggaaaatg gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt
     6241 aagaaaaaat cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt
     6301 tcaattatta gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga
     6361 gcgagaggat tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg
     6421 agtatgaaaa agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg
     6481 tttgtgtttt ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt
     6541 gcctcttctt ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta
     6601 tctgaaaaac aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt
     6661 catccgtctg ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca
     6721 cccgggacac aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa
     6781 aacaagaaag tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact
     6841 gattacttta atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag
     6901 aaagtaacgc ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa
     6961 gagaatgata cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct
     7021 gaatctttga atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc
     7081 agcaatattg ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca
     7141 aaacaatacc aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg
     7201 acgaataatc cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag
     7261 ccagagctca aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat
     7321 tttcagctgg tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca
     7381 ccggttaacg agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt
     7441 gccaaaaaga tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc
     7501 aatcaagcgg gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg
     7561 ttggctgcag atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag
     7621 tatcatcttg acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta
     7681 caattaaaat aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt
     7741 ctgaataaaa gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat
     7801 cacaatatga atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta
     7861 ttgatttaac ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca
     7921 gagaatatgc caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg
     7981 ccaaacatgg tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg
     8041 gttttatggt aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt
     8101 ggtgggggca agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg
     8161 tccacggtgt gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac
     8221 cttatgaaat aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag
     8281 agtggggcat acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg
     8341 gcagtaaata tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc
     8401 gagtattaga gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg
     8461 tgcaagaaga agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata
     8521 ttgcttttgc tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag
     8581 cagattcgat tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc
     8641 ataaaaaatt attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat
     8701 atacagtgat ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg
     8761 ttccgtctgt agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc
     8821 atgaagatga ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata
     8881 gtgaaacagt tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga
     8941 atatcggaaa aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt
     9001 tttcttatct ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt
     9061 tccggaagaa agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt
     9121 cgcattcaac tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa
     9181 aagtaacctt ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa
     9241 tttgatcaat tcagtcatca actggctact caacagttta atctttctgc ccatgttttt
     9301 aatcagcgga gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg
     9361 tgtcatgcca gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt
     9421 tgtttcagca ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa
     9481 gttcgactat cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa
     9541 agatagaatt ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg
     9601 agcaatcgct ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc
     9661 agaattttat gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca
     9721 tgtaagcagt ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga
     9781 atgtacgatg aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg
     9841 gacaacaggt tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc
     9901 tacgctgatc aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag
     9961 ccattatcat catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca
    10021 tcctaagaga aatccggaga cattgctttc aatttatgga cacacaaaag attccattca
    10081 ctttgaagaa ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga
    10141 attaaaaatt ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta
    10201 cgccatgcgt atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg
    10261 ttatttggaa agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta
    10321 tctttttgaa ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact
    10381 tgcgaaatca gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt
    10441 agaacaattg aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc
    10501 tgcagttgac aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa
    10561 accacgaccc aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag
    10621 atgaacctat tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa
    10681 atacgcttga agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc
    10741 gcttaagtgg aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca
    10801 tgcctgatga tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag
    10861 cacttcatga attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg
    10921 atggcatgaa attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg
    10981 gaacaattat gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag
    11041 acaaaatcga atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat
    11101 ttcttccaga agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga
    11161 aaattttcgg cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt
    11221 ggaaaaaaat caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga
    11281 agtctccaga tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc
    11341 atttgaatgt ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac
    11401 taggagatat ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc
    11461 tatcaaaaat agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag
    11521 aattgcttac cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg
    11581 ctatttttta tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga
    11641 aagtatcttt ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca
    11701 ttaagtcaaa acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg
    11761 tatcgtaatg tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg
    11821 acaaataaat ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct
    11881 ctaaagcgtg gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa
    11941 gatctgcttg gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta
    12001 agataagatt tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt
    12061 cttctaatat cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt
    12121 gcagattctc catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct
    12181 gtcgaagctg ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt
    12241 gtctccgatc tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga
    12301 ttagaatgat aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg
    12361 ccgtttggat gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag
    12421 ctacataaaa acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag
    12481 tgcttataac actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa
    12541 aagttgcata aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt
    12601 accatttttt atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta
    12661 agataataaa taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta
    12721 aaatatcttc tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa
    12781 gtgagtattc aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta
    12841 tagcgataaa aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa
    12901 aactaatcaa tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct
    12961 gataaggaga tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta
    13021 tcacagccaa tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa
    13081 tcttttcgcc tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt
    13141 cgtccaacgg tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag
    13201 gaatcgtacg agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa
    13261 cccacgaatc ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa
    13321 agaaataag
//
LOCUS       DADJYG010000070        13126 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.071, whole genome shotgun sequence.
ACCESSION   DADJYG010000070 DADJYG010000000
VERSION     DADJYG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.071"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            15..1754
                     /gene="yfcC"
                     /locus_tag="JZ382_001603"
     CDS             15..1754
                     /gene="yfcC"
                     /locus_tag="JZ382_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY2427792.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDEYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1911..3095
                     /gene="iadA"
                     /locus_tag="JZ382_001604"
     CDS             1911..3095
                     /gene="iadA"
                     /locus_tag="JZ382_001604"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY2427793.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3206..3409
                     /locus_tag="JZ382_001605"
     CDS             3206..3409
                     /locus_tag="JZ382_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY2427794.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3510..4172)
                     /locus_tag="JZ382_001606"
     CDS             complement(3510..4172)
                     /locus_tag="JZ382_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427795.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4185..5687)
                     /locus_tag="JZ382_001607"
     CDS             complement(4185..5687)
                     /locus_tag="JZ382_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427796.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6235..7914
                     /locus_tag="JZ382_001608"
     CDS             6235..7914
                     /locus_tag="JZ382_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAY2427797.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8041..8520
                     /locus_tag="JZ382_001609"
     CDS             8041..8520
                     /locus_tag="JZ382_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAY2427798.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8739..9419
                     /locus_tag="JZ382_001610"
     CDS             8739..9419
                     /locus_tag="JZ382_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY2427799.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9427..10758
                     /locus_tag="JZ382_001611"
     CDS             9427..10758
                     /locus_tag="JZ382_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAY2427800.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10773..11735
                     /locus_tag="JZ382_001612"
     CDS             10773..11735
                     /locus_tag="JZ382_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAY2427801.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11846..12184
                     /locus_tag="JZ382_001613"
     CDS             11846..12184
                     /locus_tag="JZ382_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAY2427802.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12263..12970
                     /locus_tag="JZ382_001614"
     CDS             12263..12970
                     /locus_tag="JZ382_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAY2427803.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4168 a         2319 c         2889 g         3750 t
ORIGIN      
        1 aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgt gacttcacta
       61 aattatcgac accgcatacg tatgtcatta tttttggtgt agtcatcttt gcatggatac
      121 tgacttttgt cgtaccagca ggaaagttca gcacgcaaga cattgaatat aaagatgcga
      181 atggcgaaac aagtactcgt actgttttac gccaagattc cttccgttat gcttacgagc
      241 tggataaatc gtatgtattt gatcaattag aagaactgca agaccatcca gcagaacgtg
      301 aaaaattgga tgttcctgaa aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc
      361 ttacgcaaga aaaactagat gaaatttcct taacagatga tgttctttat gatgagtatg
      421 gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata tggggaacag
      481 acgattttgg tggctttggc ttcttaaatt ttgtttttga aggtctagta tctggagata
      541 aatatggatc tgctgtagga attgctgcat tgatccttgt tgtcggaggt gcttttggta
      601 ttattatgcg taccggagcg attgatgcag gaatctatgc gtttatcagt aaaacgaagg
      661 gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt ggcgctacat
      721 tcgggatggc agaagaggta attccattct caatggtgat ggttccattt gtcattgcgt
      781 taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg
      841 cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct ggaatccccg
      901 tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca ttagccgctg
      961 gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca
     1021 aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat aaaaaattca
     1081 tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg atcgtttggg
     1141 gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt gtgatgggat
     1201 tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc aatgacatcg
     1261 cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta gtaggtatgg
     1321 ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca gctttgaata
     1381 cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt ggtgcttggg
     1441 cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca ggacaagcag
     1501 ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc agacaagtag
     1561 cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc gtatcagcca
     1621 gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca agattccaaa
     1681 tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt gccattgcaa
     1741 taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca gtagataagg
     1801 actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat cgtttggatt
     1861 attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt atgaaaatca
     1921 ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta ctgattgccg
     1981 gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga gtggaagtag
     2041 aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt catttccata
     2101 ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc acattgagcc
     2161 aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac ggagaaggta
     2221 gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga atctccactt
     2281 atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct atcatcaaag
     2341 actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc gatcaccgtt
     2401 cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt gtaggaggca
     2461 tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa agaaagctag
     2521 agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt ttgcctactc
     2581 atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa cgtggtggca
     2641 cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat ggtgaagttc
     2701 gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat aatttcacga
     2761 tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat ttcctagggt
     2821 taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg caaaaagaaa
     2881 gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt atattgaaat
     2941 tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc ttggataaag
     3001 aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa gaaaaagaag
     3061 tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac agctctaact atctttttta
     3121 gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt taccctgaag gtagatcaat
     3181 aaacaaacta agaggaggaa gcacaatggc agatttaaaa ggacgttcta aagatatgaa
     3241 agataaagta gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg acaagagcaa
     3301 agaaacagaa gggaaagcac aatcactagg tgctgacatc aaagaaaaag ctcgaaattt
     3361 aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa aaccacttat
     3421 tactaattga atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag atatggttcg
     3481 gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa ttttttattt
     3541 tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt cacgatgata
     3601 atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt
     3661 gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc acagcagata
     3721 gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac ctgttttttt
     3781 gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg tcgataattc
     3841 tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc cgataaataa
     3901 ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata acttcttaac
     3961 ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg tgcatctagt
     4021 ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa aatagcgtac
     4081 atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg cggatcttga
     4141 taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt taataggatt
     4201 tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa cagactgatt
     4261 ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat ttccaattcg
     4321 ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc atcgcgttga
     4381 ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg ctggttttgt
     4441 gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc gacgacgttt
     4501 ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc ggtaaacaat
     4561 tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa ctcatgccga
     4621 cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc tacagccact
     4681 agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa cattgactga
     4741 tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt ctcagcttct
     4801 ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga atctatcgtg
     4861 ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta aagtgtattc
     4921 gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct ggattcataa
     4981 attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt gattgtgaaa
     5041 gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac tacattgttt
     5101 gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt tccatcagaa
     5161 aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc ttgagtcatt
     5221 tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt ttctgaactg
     5281 tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat agctgaggcg
     5341 gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc cgcgtccgtt
     5401 aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt ggtagaaccg
     5461 cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact taaagtcact
     5521 gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg
     5581 aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa caatgaacgt
     5641 ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt ttgcatccct
     5701 cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa tcttatgctg
     5761 tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt tattgcaaaa
     5821 aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga aagaggaata
     5881 gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt ctaacaattg
     5941 ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg aatacttata
     6001 taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag tatatccaaa
     6061 ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct gctgattctc
     6121 cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat atatttagat
     6181 aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt
     6241 accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca
     6301 gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga aaatgagttg
     6361 ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat tgatcgtgta
     6421 gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc ttacttgttg
     6481 tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc aaaattgaat
     6541 gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga tgagcataca
     6601 gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac gataccagat
     6661 tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg agattttaaa
     6721 tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc ggaaatcggc
     6781 aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta
     6841 gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg ggaagggcgt
     6901 atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt ggatgcagca
     6961 gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca
     7021 gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac gttgaaagac
     7081 atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat gggtgaaccc
     7141 atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat tgaagaagga
     7201 gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg
     7261 gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt
     7321 tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa accaacatat
     7381 tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt ggctcatgca
     7441 gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct ggaatataag
     7501 aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat tgatggatta
     7561 ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc agaagacggg
     7621 attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa
     7681 attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc
     7741 aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga gtggagcaaa
     7801 ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac aaaacgccgc
     7861 ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa ataaacaaaa
     7921 agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg aaaaacaaat
     7981 aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg gagtgaatca
     8041 atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag ctatcaacca
     8101 cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa aagagggatt
     8161 gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc
     8221 gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt gacgaaagat
     8281 cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata tccccacgga
     8341 gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt agcagacagt
     8401 tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag aagccttcgg
     8461 ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt aattagttaa
     8521 gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat atctgcttgg
     8581 tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta ctatcaatcc
     8641 aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg aaatcgtttt
     8701 taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg tatcatggac
     8761 attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac ccgtttatta
     8821 caggaaatcc attgacagac ttaactgcag aagaagtaaa gacagattgg ttgctgatta
     8881 ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa aatcaagcca
     8941 caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta gattcattcg
     9001 gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc gtacatgcac
     9061 agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag cctagcggaa
     9121 ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct tatttcagtg
     9181 atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg attggcgata
     9241 actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt gccaagaaag
     9301 tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa tcttttgtag
     9361 agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg
     9421 cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt acctgttggc
     9481 aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac
     9541 cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg agtagggacc
     9601 atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc
     9661 aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt cttgaataaa
     9721 ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc aggatcacta
     9781 atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg cgccgcagta
     9841 ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga tgatcttgaa
     9901 atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat caaccgagcg
     9961 ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta tatgccagct
    10021 gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg tttgtcagaa
    10081 gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat ggggattgtt
    10141 acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa
    10201 gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca aatgatctgg
    10261 gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg cttgattacc
    10321 cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat tgcagatacg
    10381 atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga aggaaatcgt
    10441 ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg cgttggaacc
    10501 atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg
    10561 aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg gctgatccaa
    10621 ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa
    10681 ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa
    10741 gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa atagtagata
    10801 caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat cctgatgcat
    10861 taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa aaagaaatac
    10921 gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa gaagttgaag
    10981 atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca cccagaatca
    11041 gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg
    11101 acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt gagatgattg
    11161 cagaatttgc tttgatgttt cctgaatctt taaagatgaa cgttaaggct gcacggttac
    11221 tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg acgtcccgta
    11281 cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct ttgaacagag
    11341 agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat caaaatatcc
    11401 aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt gataaatatg
    11461 gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc
    11521 tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt ttacgttcta
    11581 aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat ccattagcaa
    11641 gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag atccaagata
    11701 tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt
    11761 gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca atagtgctca
    11821 ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat gtggctcaga
    11881 gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa
    11941 agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa atggaaaccg
    12001 tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg
    12061 tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg atcataatat
    12121 cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa
    12181 ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata aaataaagta
    12241 aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt tagatccgac
    12301 atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt acgtcaatag
    12361 tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta cacaagcagc
    12421 acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa ttgccggaga
    12481 tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag caactgcaca
    12541 gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg cggtacaaga
    12601 tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag ctaactttgg
    12661 ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga actctacgtt
    12721 aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg tgacattacc
    12781 agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg gactactgac
    12841 ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga cttatgttgc
    12901 agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg
    12961 aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa taccgagagt
    13021 tagctgattg aataccattt atttggaata agggctcttt gtcaataagg actgatgggt
    13081 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcag
//
LOCUS       DADJYG010000071        12826 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.072, whole genome shotgun sequence.
ACCESSION   DADJYG010000071 DADJYG010000000
VERSION     DADJYG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.072"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            105..1280
                     /gene="mltG"
                     /locus_tag="JZ382_001615"
                     /pseudo
     CDS             105..1280
                     /gene="mltG"
                     /locus_tag="JZ382_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            1416..1895
                     /gene="greA"
                     /locus_tag="JZ382_001616"
     CDS             1416..1895
                     /gene="greA"
                     /locus_tag="JZ382_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY2427804.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            2064..2795
                     /locus_tag="JZ382_001617"
     CDS             2064..2795
                     /locus_tag="JZ382_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAY2427805.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            2792..3859
                     /locus_tag="JZ382_001618"
     CDS             2792..3859
                     /locus_tag="JZ382_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY2427806.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            3866..4498
                     /locus_tag="JZ382_001619"
     CDS             3866..4498
                     /locus_tag="JZ382_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2427807.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            4638..5303
                     /locus_tag="JZ382_001620"
     CDS             4638..5303
                     /locus_tag="JZ382_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAY2427808.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            5331..5624
                     /locus_tag="JZ382_001621"
     CDS             5331..5624
                     /locus_tag="JZ382_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAY2427809.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(5692..6639)
                     /locus_tag="JZ382_001622"
     CDS             complement(5692..6639)
                     /locus_tag="JZ382_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAY2427810.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(6817..7620)
                     /locus_tag="JZ382_001623"
     CDS             complement(6817..7620)
                     /locus_tag="JZ382_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAY2427811.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            7766..8659
                     /locus_tag="JZ382_001624"
     CDS             7766..8659
                     /locus_tag="JZ382_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAY2427812.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKNLFF"
     gene            8727..9197
                     /locus_tag="JZ382_001625"
     CDS             8727..9197
                     /locus_tag="JZ382_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY2427813.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            9282..9890
                     /locus_tag="JZ382_001626"
     CDS             9282..9890
                     /locus_tag="JZ382_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAY2427814.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            10024..10224
                     /locus_tag="JZ382_001627"
     CDS             10024..10224
                     /locus_tag="JZ382_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY2427815.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            10978..12204
                     /locus_tag="JZ382_001628"
     CDS             10978..12204
                     /locus_tag="JZ382_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAY2427816.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(12312..12782)
                     /locus_tag="JZ382_001629"
     CDS             complement(12312..12782)
                     /locus_tag="JZ382_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAY2427817.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         4438 a         1961 c         2605 g         3822 t
ORIGIN      
        1 ctacttacat ttttgataga atatttaaat aaaatcattc acgccaattc aaatatcatg
       61 tataatgaat aaagttacga acttgatagt aaggaggagt tgttttgtca ggaaacaaac
      121 aacacccaag taaagataaa aaagaagcca gtaaggcagc tacagcagat ataatcgaca
      181 agcggagaaa tctgcgtaaa aaagaagata agattgtgag gaagatcatt ttagtgattg
      241 ctcttacatt attgattatt ggcggatttc ttggatttac cgtgtatcgt tatgtagata
      301 gcgggttgaa gcctttagat aaatcagatg accaattagt ccaagtcgag atcccgagtg
      361 gctcttctaa caagcagatt ggtgagattt tggagaagga caatattatt aaaagcggga
      421 ttgttttcaa ttattataca aaatttaaaa atttaactgg ctttcaagct ggctattatc
      481 agttagcgcc taatatgact cttgacgaga ttggcaaaca gttgcaagaa ggcggtactt
      541 ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaagggtac gatatcgatc
      601 agattgctga acgggtagcg aaggtaactg gtaaagacaa gaaagaattt ttagatttag
      661 tgaatgatga gactttcttt aacgagttgc accaaaaatt cccagaactg ttggaaagtg
      721 catctaaagc tgagaacaca aaataccgtt tggaaggtta attattccca gccacatatg
      781 attattacac gagtacttcg cttaaggatc tagtgattga aatggtgaac aagacgaata
      841 cggtcatgca aaactattat tcagctatca agcagaaaaa tttgactgta caagaagttt
      901 taactctagc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt aaaaacattg
      961 cacaagtctt ctttaaccga atcaaagcaa atatgccact tcagtcagat atctctgttt
     1021 tatacgcatt aggtgaacac aaagaattag tgacgtatga agatacagct gtagattcac
     1081 catataattt gtataccaat accggctatg gacctggacc ttttgacaat ccaagcgaag
     1141 aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc gtagcagata
     1201 cgagcactgg aaatgtttat ttcgctaaaa cttatgaaga acatatggaa ttagtggaaa
     1261 aatatgtaaa taattcttag aaaaacaggc tctagacatt gatcattttt agaaaaacat
     1321 ttacatttga agaaaagcgt ggtaatcttt tttggactat attcctcaaa agattacctt
     1381 ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt tatccaatga
     1441 ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca gtcaaacgcg
     1501 gagaaatcat tgaacgaatc aaaattgccc gcggtttcgg agatctttcc gaaaactctg
     1561 aatatgaatc agcgaaagat gaacaagcat ttgttgaagg aagaatcaca acaattgaaa
     1621 atatgattcg ttttgctcaa atcatcgata acgacggtgt agatgcagat gaggtatcaa
     1681 ttggtaaaac agttacattc atcgaactac cagatggaga agaagaagag tacacgattg
     1741 tcggaagcgc tgaagcagat ccgttttctg gcaaaatttc caatgattct cctattgcgc
     1801 aagcattgat tggcaaacgt aaaaatgatg aagtaactat tgctactcca ggtggcgata
     1861 tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc tagagagttc
     1921 atcttactga tgatacactc tagatctttt tctctttgtg tattttagat ttcaacaaac
     1981 gatcttacat ctaaagtcct atagccaaca gttaaaaaaa taggtatgat gaaatgagaa
     2041 tgtgtcagga gggagaaaga gcgatgaata gttcttggcg tttttttgtg gtagttgaag
     2101 cactactgct gttatttgct gtttggcaaa tcgtaaacaa tactggactt ttgcttttag
     2161 tgatattcgg tattttcaat atatatctag ctatgcgcaa acatcctcgt actaaatttc
     2221 aaaattttca attgatatta ggaagtttgg tcattttttt tagcttagtc aacagtcctg
     2281 ctttatggat gatggctgta tttgccgttt tatttattgg tctgaaaggt gtagagattt
     2341 cagggataga tttaaccaaa aatgcttttt ggcgaaagaa acagatcatg atgatccaaa
     2401 cagagcaatt aaaaacacat aataatgagc ggaaaagaca gcaattattc ggtaaccaac
     2461 gtattggaaa cgacgtctat gaatgggacg atatcaacat cgccgtcatt tctggtgata
     2521 ctatcataga tttgggcaat acgcttttgc caaaagatga taatattgtg attgtaagaa
     2581 aagggatagg cagaacacgg atcttagtac ctttgggcgt agccatccga ttagagcatg
     2641 cgacattagt aggaaatgtc ttgtttgaag aagagcaatt ttctttgaaa aatgaacaaa
     2701 tcaaaatcta cagcagtgat tatgatgaaa accctcgtag attgaagatt attactaata
     2761 ccttgattgg agatgtcgag gtgatacgaa tatgatggga aaaatatcca gagcgatgct
     2821 agctgtttat tcggggattg cggctttcct tattattcta ttttcacttt tcacttattt
     2881 ttatgccagc aatcaaagtc attggtgggg agaattactg cgtgcacgtt tattatatgt
     2941 tccgcttatc ttccatttgt tagccatttc cttaggtgta ggattaattg tctttctatt
     3001 attatcactt attcaaaaga caaaatacgg gaaaatcgaa gaaaagctgc gtgcactttc
     3061 ttctggcaat tatgaatcca aattgttgct tcttccgatc ccaagtgcat cggacgattt
     3121 atacatcaaa gatattgata aggaaatcac taagataaaa gaaaaaatga ttgaagtatc
     3181 tagtgaatta caaattgtaa cgagccgtcc ccaatatgta gatggacaaa ccaaagaaga
     3241 aattttagaa ttagaaagac atcgattagc ccgtgagctg catgattcag tttcgcaaca
     3301 attgtttgca gcaatgatga tgatgtcggc attgacggag caagcagaaa aaagcgagac
     3361 accagaaatg tttcgcaagc agttgaaaat ggtagcagag atcatcaacg cttcccagtc
     3421 tgagatgcgt gcgctgcttc ttcatctacg tccagtcaat ttagaagaga aaagtttaaa
     3481 gcaaggtatc gagcagttat tgaaggaatt gcaaaataaa atccagattt cgctgaaatg
     3541 ggatgtagaa gatgtaaaac tatctagttc cattgaggat cacttgttcc gaatcgttca
     3601 agaattgtta tcaaatacat taagacatgc taaagccaat gaattagagg tatatttgca
     3661 caaaatagac aataatcttt tgttacgtat tattgacgac ggaacagggt ttaatatgaa
     3721 tgaaacgaaa acgggaagtt atggattgaa caatatcaaa gaacgagtag ctggtattgg
     3781 tggaacagta aaaatcatta gttttaaagg tcaaggtaca agtgtagaaa tcaaggtccc
     3841 tttgatgaag gaggcataga taaggatgat aaaagtttta ttagtagatg accatgaaat
     3901 ggtgcgctta ggcgtctctt cttatttatc tattcaagaa gacattgaag taattggaga
     3961 agctgaaaat ggacgacaag gctatgagaa ggcgatgaca cttcgaccgg acgtcatttt
     4021 aatggactta gtcatggaag aaatggatgg tatcgaatca acaaaagcaa tcttaaaaga
     4081 ttggccagaa gctaaaatca ttatcgtaac cagttttatt gatgacgaaa aagtttatcc
     4141 tgccattgaa gcaggtgcag cagggtattt gttgaagacc tctaccgcac atgaaattgc
     4201 tgatgctata agggcaacgc aacgtggcga gcgagtatta gaaccagaag tgacaacaaa
     4261 aatgatggaa aaaatgagtc ggcgaaatga gcctgtatta catgaagaac taacgaatcg
     4321 ggaaaatgaa attttaatgc tgatttctga aggaaaaagt aatcaggaaa tagcggacga
     4381 attgtttatt acactgaaaa cagtaaaaac acatgtttcc aatatattgg ctaagctaga
     4441 agtggaagac cgtacacaag ccgctatcta tgcattcaaa catggtttgg tgaaataaaa
     4501 acgatgataa agaggataat caaaagctga gaaaataact attatttttc ttggcttttt
     4561 tacaataatg atgcttgctt ttgtttttgt aaaggctttg ttatactgag aaaagaagat
     4621 actaaaggag gaaaacggtg aaacaaaatt ttgcaattgt aggtcttgga cgatttggcg
     4681 gaagtatctg ccgaacactg atcgaagctg gtcaagaagt actggctatt gatagtaatg
     4741 aagatcgggt caatgaatat atgaatatag ccactcatgc tgttgtggca aatgcacagg
     4801 atgaaatgac acttcgatca ttaggcataa gaaatttcga tcatgtaatt gtcgctattg
     4861 gagaagatat tcaagccagt atcttagtta ctctgatggt aaaggaaatg ggcgtaccaa
     4921 atattttagc aaaagcccaa aatgagtatc atgccagagt acttgaaaag atcggagctg
     4981 atcgcgtcgt ccatcccgag cgagacatgg gaataaggat tgcgcataat ttagtgtcaa
     5041 agaatatttt agattaccta gagttgtcag ataaatattc tttagcagaa atccgagtct
     5101 ccaatcctaa atttttcaac aaaacattag ctgaattaaa tttcaggcaa cgttttgact
     5161 taacagtagt cgccatacga agagtagatc aaacggttat cgcttctcct gcagcagatg
     5221 aatatgttcg tgaaaatgat aatctactgg ttattggaga gacaggcgat gtggatattc
     5281 tagacgataa gatgaatcaa tagaaagaaa agagaaagta ggttgttggg atgaaattaa
     5341 cggtaacgcc aaaagcacaa aagtggtttg actcagaaat tgatctgcca gaaacttacg
     5401 gaatacgttt ttttggaaaa gtctacggca aaacagaggt tcatgatggt ttttcgattg
     5461 gaatgtcagt tgaacagcca gaacatcctg taaagaaagt ggaaatggat gggacacttt
     5521 tttttatcga ggaagcagat gaatggttct ttaaaggata tgatttactt gttgattatg
     5581 atcccaacct ggaggagcca acctatcact ttaaaaaaca ataaaaaaag agagggtgac
     5641 acaatttttg tcaccttctc ttttttcttt tgaaaatatt gttttcagct attaatatcc
     5701 tgcagacaat tttacttgat tggtttgaag ctcatgggtg gcaaagtagc ttttcagatt
     5761 ggttaaaaaa atagccatta atttcttttg aaaatcaggt gtcaaacctg caatgtggga
     5821 agtaatcagt acattgtcca ttttccaaag aggatgatct tgtggaagag gctcttcctc
     5881 aaaaacgtct aatgctgcaa aggaaagttg ctggttattt aaagcctgca ccagatcttg
     5941 tgtcttaaca gaagcaccac ggccaacatt gatgaataca gattttggat caaagtgttc
     6001 aaacatatgc tgatcaaaga gcccagttgt ttcctccgtt agaggtaaga tattgactac
     6061 gaaatctact tcaggaagaa cttcctttag ttgatttaaa gaaaaagtct tttccattcc
     6121 ttctgctgga tgaccggatg tattgattcc atagcagttc atgtctaatg ctttgcattt
     6181 ttttgcaatc atttttccaa tatggccagt tccaatgatt agtatttttt tcccttggat
     6241 agattggtaa tggaccgatg aacctaacca tttcatgtca ctctgtgcct tttgtgcttg
     6301 aaaaagacca cgagaatacc caaggatgat tcctaagaca tgctccgtaa tcgattcact
     6361 gtggatgcca ctaccatttg aaagtaaaat atgtttcttt gaaaaagtct caaggggtag
     6421 ctgatcaaca ccagctgaga tagattggac ccatttcaag gtagaagagg ctaatagttt
     6481 ttcttggtag tctgagttcc agcccaaact gatctctaca ttttgtagat cctctggttg
     6541 taattctgtt ttaaagatat aaccaggcgc tatagtatgg atttcttcga taaattcatc
     6601 acgaaatgga cgatctgcat aaatgattct ttctgacata gttaatttcc cgcctttctt
     6661 tactctaagt gtagcgcata caagaagaac tttgaagcgc cttgcttgtt ttttgagaaa
     6721 gaaatatttt tcaaataaaa aaggggctgt gacaaaaaaa tgtcacaacc tctaaaaggg
     6781 tctattgaag tagaaataac gagttgtctt tatttcttaa ttgatcgata tttttgccca
     6841 tcctttgaca gcttgagcaa aaataaagat tcctgcaaaa gcaatgaagg cgctagtgtc
     6901 aaaagaagga agaaagacca tcaagattgt tgcaattata gtaggcaatg aaattgaata
     6961 gatcaatgtt tttaaacaat caagaaaagt agcttttgtt agtcgaaagc gtgcatagag
     7021 aaaagcagca atgttggtaa atagcaaagt gatcagcaga ttcagaaaac taggataaat
     7081 agaaaatata aatgtcagga cttttatcca aagtggtata gatgcttcac ttaaaaaaga
     7141 acggagctgc ttacctgtta agttttttaa agattcattg gtgtagggaa gttctaattg
     7201 gttattgttc aacaaagcag aagtcgtgcc tgtattagga agagctatga taagtttgtt
     7261 ttttaacatc ccgacactaa caaaattccc catcaaatca gtagagatgt cctgttctgt
     7321 tcgttttccc tctggatcaa atgtgaagat aatggagttt gtttggtaaa tgaatccttc
     7381 tttttcttcg gtttgaagct gcccgttttg gattgtaaaa tcaggaatgc gagtagcaat
     7441 cttttgccca tccaattgaa tgttgtttaa gacttgaaaa acttgaaaag agattggcaa
     7501 agccataatt atactcaaaa ctaaaagata gacaatggct ttccaaaatg ggacatttct
     7561 cgcctccttt aggtctttga atcgtataaa ggaactgata attaattgtt ttgttgtcat
     7621 aagtactcct catctagtag tgtcatcact tgcaattcta gcgaagtatc aagtgaaata
     7681 caagtgcttt aaggaaacag ctgaatgatt ggtgaaatat caaaaaaatg ttattcttgt
     7741 ctaaagacta tccaatataa ataaaatgga ggaaggaata gtgcctacac agccacagag
     7801 atcatcaaaa aaactatttg gagtagtggt tttgaatgcg gtgatcactt tatcagaatt
     7861 tattggaggg attatgtcag ggagtttgtc tcttgtttca gatgctttcc ataaccttag
     7921 tgatacattg gcaattattt tttcttacgg ggcacaaaaa atggcccgaa aagaagcaaa
     7981 taaaaaacgt acgtatggct accagcgttt agaaatattg agtgctttca tcaactcatt
     8041 catattgatc atattatcct tgtttttaac cgttgaagca ttcaagcggt tcacttcacc
     8101 agaaaagata aatagtcatt tgatgctaac agtagcagtt attggattgc tcgcgaattt
     8161 gttctcagta ttattattac gacaagaagc agatgaaagt ttgaatataa aatctagtta
     8221 tttgcatttg ttaagtgata cactgtcttc ggtaagtgtg attatcggag caattttgat
     8281 ccgttttttc gatatctact ggatcgatcc agttattaca ctgatcattt cgatatatat
     8341 attgatagaa gcgataatgg tgatcaaaaa agctgccgct attttgatac aaagtgcacc
     8401 tgctatcgac tatgaaaaaa tggaacaaga aattgaagca atcgatgggg taaaggatgt
     8461 tcatcatgtc catatttggc agtatagcga aaagataatt attttcgacg gacatattga
     8521 ttttgaagat cagctactaa gcgagattga aaagatatat cctcgtataa tttctttatt
     8581 aaaattaaaa tatggtatta cacatgtgac gatccaagca gagacaaagg caaaggatca
     8641 aaaaaattta tttttttaaa taaagatgaa agttattgaa atatagagta tgataaataa
     8701 gaatttttaa taaggtaggt tggctcatga agcagtatcg acagttaaaa ggatatttag
     8761 aagcaaaagg atattgggag atactagtct atctcttttt tggtggtttg gctacgattg
     8821 taaattttgt tagttttgct ctcgcacgcc aatattttga tttaagtatg gccttatcca
     8881 attctatttc gtggttttgt tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat
     8941 tccattcaaa atcaccgaat tttacacatg cacttattga atgcggtaag ttcttcttct
     9001 accgtatcct ttcttacgga ttggacatgg gggcaatggt attgttgatc aatgttatga
     9061 acagcaatga gtatgtggcg aaaatcatca ctcaaattat tgttatcctt gcgaactata
     9121 ttttcagcaa gttgtttatt tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa
     9181 gtgaaaaaga cgactaggca taacaattga agaatttcct tctctttgtt actctttgta
     9241 atgtagacat tattaagaaa acattgagga ggaatttttt tatgacttac acattaccag
     9301 atctacctta tgcatatgac gcattggaac cttatattga cgaagaaacg atgcatctgc
     9361 atcatgataa gcatcacaat acttatgtga cgaatttaaa ttcagcaatt gagaaatacc
     9421 cagaattagg cgaaaaaaca atagaagaat tattatctga tatggacgct attccaacag
     9481 atatcaagac agctgtacgt aacaatggtg gcggacatgc taaccattca tttttctggg
     9541 aaatcatggc accaaatgct ggtggcgaac ctacaggaga aataaaagaa gcgattaatg
     9601 aagcttttgg tgatttttct tcttttaaag aagaattcaa aaaagcagcc gctggacgat
     9661 ttggttctgg atgggcttgg cttgtaatgg aaaatggaaa attagctatt acctctactg
     9721 caaatcaaga ttctccattg atggaaggaa agacaccaat tctaggtttg gacgtttggg
     9781 aacatgctta ttatttgaaa tataaaaatg tccgaccaga ttatattgca gcattctgga
     9841 atgttattaa ttgggatgaa gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc
     9901 cgtcatatca gtcttaaaaa taatttggaa aataaaagag cgaaagcttg ctagcgtttt
     9961 ctaaattggg tataatgttc ttgcaaggtt acttgcaaga acttttttgg aggatataca
    10021 tttatggaac acggaacggt taaatggttc aacaatgaaa aaggatttgg gtttattaca
    10081 gtagacggcg gagatgacgt atttgttcat ttctcagcaa ttcaaggaga cggcttcaag
    10141 agcctagaag aaggtcaaga agtagaattc acgattgttg aaggtgcacg cggacctcaa
    10201 gctgctgaag tgactaaatt ataataattt gttgaataaa gacacgactt cttagagagt
    10261 cgtgtctttt tgtctattta tgaagttttt agattttttg gctaagtaaa tgcatttgtt
    10321 cacttgacat gtcctaacta attatcaagc aaggaacatt ttcttcgtta gtaatggatt
    10381 ttaaagaaat ttcagttttt ggatgttgaa taaaagacca tttgtgaaac cactagtata
    10441 catactcatt accatgtcaa ttctattact ttaaaagaaa atcgttttac tttgaagagg
    10501 agcatatcta tccattaaaa gatgatggta atgcaatcac tagttcgagt cattttaaga
    10561 gccatgcagt gagctggaaa taaaacgaat agaagatggg tttaacaaaa ggaccagtca
    10621 taggaagaaa ttgtcgtgaa atcgaatgat aatctgaacg gtaggcaaag actgttaaaa
    10681 agatgggaaa ggtatgtgcg atcattttac tgcacgatta tttggaaaat accttgttgt
    10741 tcgctttttc tggaaaaaat acataaaaaa agataaaacg attgcgaact gcgtgtattc
    10801 ggtctttcga tctttttcac ggtatggtat gatagttaag aattttgaga aattttaaaa
    10861 aataataatc gttacacaaa aatgtttttt aaagttttct tatgattaaa aagtgaattt
    10921 gctgattgaa cagttatatg agacaatatt ttagaaaatg tgaaacagag gtgtattatg
    10981 aagcgatggc aaaaagttct attaaccttg ttaggggtat tcgttttgct gattgccgga
    11041 ttatcagcat acggaatcag atttatggga gaagcgagcc aaacagtaaa caaaatttct
    11101 aaaaactcta atcgaatatc ctccaaacgt tctgagagag taagcattga tgacaaagaa
    11161 cctttttcgg ttttgctatt aggtttagat accggtggat taggaagaac ggaacaagga
    11221 cgttctgata cgatgatggt agtaacagtt aatccgaaac aaaaaaaatc aacgatcatc
    11281 agtttggacc gagatattta tacgaatatc gtaggttatg gaacggtaga taaattaaat
    11341 catgcctacg catttggtgg tgtcgaaatg gccatggatt caattgagca actgctcgat
    11401 attcctattg atcattatgt aacgatcaat ttggatggca tggaagattt gatcaatgcg
    11461 gtcggtggca tcaaagtgaa taataaaatt gactttactt tggatggtgt ccatgtacca
    11521 gtaggtgaac aaacattgaa tggagaaaaa ggtttagcct attctcgaat gcgacacgaa
    11581 gatccagaag gcgatatcgg tcgacaacgt cgtcagcggg aagtagtgac gaaaatcgtc
    11641 aataaagttt taagtttaga tggcgtaagc aattatagaa aaattttaaa agcagttgag
    11701 aacaatgtga cgacggactt agattgggat gacatgcttg atgtagcgac gaactacacc
    11761 ccggcgtttg aaacaatcaa acaagaccag ttgcaaggcg aaggacaaat gatcaatgat
    11821 atctactatc aaattttagg aaaaaatgat cttctttcga tccaaaatga actgaagaaa
    11881 caattaaata taaaaacaag cgatacatta cctaatttga aaaatgagaa tgcttcaatg
    11941 atgttctttg atgattcgga agatggcgat tcgacaaacg gtgatggatc gaatagtgaa
    12001 gggtacaccg attattcttc ctattcagaa aacacatatg atcagtacca agatccgaat
    12061 acctattatc agcaaacaga agatacaagt cagtactata caccaagtca agatactcaa
    12121 agttatgatc cttacgctgg acaggaacaa aatggttatg gttatgacca gaataatgag
    12181 tatggaacaa caagtgggta ctagaaaaaa atagtttggt taataaagag tgccacctaa
    12241 attagacaaa aaatctaact taggtggcac tttttctatg tgttaaatta caggacgtct
    12301 ctttttagcg cttagtttaa aaaataaatt gaataattca atagagttgc gaatagtacc
    12361 cataaataat aaggaatcat gagccacatt gtcaggcgat gatgcagcca caactgatac
    12421 ataagcactg acaagaaaat aactaaaagt gtaatatcga ttgctgcaat gaaaatattt
    12481 cttaaattaa aaaatagagc agaccatata aaattaaaaa tgaactgaat cacaaaaaga
    12541 ccgacaagat tgtttcgctg ttttttgttg ttgtgattaa gtaacaaata aagagaaata
    12601 cccatcaata tatacagtaa ggtccacatc ggcccaaata tccagctagg tggtgcaaat
    12661 ggtggaagtt gcaaagaata gtaatatttt ccgggatttc cgctaagcaa ccctgataaa
    12721 aagcctaata tgacaattcc tataacacaa acccaaaaac gaatatcttt tatcatcttc
    12781 attttactaa ctcctttcta taatattagt ttaccatgaa ggctca
//
LOCUS       DADJYG010000072        12734 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.073, whole genome shotgun sequence.
ACCESSION   DADJYG010000072 DADJYG010000000
VERSION     DADJYG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.073"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="JZ382_001630"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="JZ382_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY2427818.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="JZ382_001631"
     CDS             complement(322..891)
                     /locus_tag="JZ382_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAY2427819.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="JZ382_001632"
     CDS             1077..1979
                     /locus_tag="JZ382_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2427820.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="JZ382_001633"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="JZ382_001633"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY2427821.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="JZ382_001634"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="JZ382_001634"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY2427822.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="JZ382_001635"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="JZ382_001635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY2427823.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="JZ382_001636"
     CDS             8547..8732
                     /locus_tag="JZ382_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAY2427824.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="JZ382_001637"
     CDS             complement(8794..9990)
                     /locus_tag="JZ382_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY2427825.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="JZ382_001638"
     CDS             complement(10160..11596)
                     /locus_tag="JZ382_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY2427826.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="JZ382_001639"
     CDS             11889..12698
                     /locus_tag="JZ382_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2427827.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2674 c         2248 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat aatcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DADJYG010000073        12724 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.074, whole genome shotgun sequence.
ACCESSION   DADJYG010000073 DADJYG010000000
VERSION     DADJYG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.074"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            11..733
                     /locus_tag="JZ382_001640"
     CDS             11..733
                     /locus_tag="JZ382_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAY2427828.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="JZ382_001641"
     CDS             791..2830
                     /locus_tag="JZ382_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAY2427829.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="JZ382_001642"
     CDS             complement(2939..4090)
                     /locus_tag="JZ382_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAY2427830.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="JZ382_001643"
     CDS             complement(4244..4774)
                     /locus_tag="JZ382_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427831.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="JZ382_001644"
     CDS             complement(4873..5223)
                     /locus_tag="JZ382_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAY2427832.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="JZ382_001645"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="JZ382_001645"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY2427833.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="JZ382_001646"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="JZ382_001646"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAY2427834.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="JZ382_001647"
     CDS             complement(7626..8288)
                     /locus_tag="JZ382_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2427835.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="JZ382_001648"
     CDS             8472..10118
                     /locus_tag="JZ382_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY2427836.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="JZ382_001649"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="JZ382_001649"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY2427837.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..>12724)
                     /locus_tag="JZ382_001650"
     CDS             complement(11299..>12724)
                     /locus_tag="JZ382_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAY2427838.1"
                     /translation="MSESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPA
                     YCYYRLFGRYCNESVSYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLAL
                     YGLSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFK
                     KEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPN
                     LPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFD
                     LFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYM
                     DFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVK
                     IEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRI
                     SEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4257 a         2347 c         2358 g         3762 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatatattc
    12121 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    12181 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    12241 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    12301 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    12361 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacaa
    12421 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    12481 ttgctgactt aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    12541 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    12601 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    12661 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    12721 cata
//
LOCUS       DADJYG010000074        12634 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.075, whole genome shotgun sequence.
ACCESSION   DADJYG010000074 DADJYG010000000
VERSION     DADJYG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.075"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(238..600)
                     /locus_tag="JZ382_001651"
     CDS             complement(238..600)
                     /locus_tag="JZ382_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427839.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(674..1054)
                     /locus_tag="JZ382_001652"
     CDS             complement(674..1054)
                     /locus_tag="JZ382_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAY2427840.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1136..2311
                     /locus_tag="JZ382_001653"
     CDS             1136..2311
                     /locus_tag="JZ382_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY2427841.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2388..2972)
                     /locus_tag="JZ382_001654"
     CDS             complement(2388..2972)
                     /locus_tag="JZ382_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY2427842.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3173..4171
                     /locus_tag="JZ382_001655"
     CDS             3173..4171
                     /locus_tag="JZ382_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY2427843.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4283..4792
                     /locus_tag="JZ382_001656"
     CDS             4283..4792
                     /locus_tag="JZ382_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY2427844.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4822..5481)
                     /locus_tag="JZ382_001657"
     CDS             complement(4822..5481)
                     /locus_tag="JZ382_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAY2427845.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5483..6247)
                     /locus_tag="JZ382_001658"
     CDS             complement(5483..6247)
                     /locus_tag="JZ382_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAY2427846.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6255..6932)
                     /locus_tag="JZ382_001659"
     CDS             complement(6255..6932)
                     /locus_tag="JZ382_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAY2427847.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6969..8354)
                     /locus_tag="JZ382_001660"
     CDS             complement(6969..8354)
                     /locus_tag="JZ382_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAY2427848.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8323..8577)
                     /locus_tag="JZ382_001661"
     CDS             complement(8323..8577)
                     /locus_tag="JZ382_001661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427849.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8564..8648)
                     /gene="ffs"
                     /locus_tag="JZ382_001662"
     ncRNA           complement(8564..8648)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JZ382_001662"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="JZ382_001663"
     CDS             complement(8671..9186)
                     /gene="tadA"
                     /locus_tag="JZ382_001663"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY2427850.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9293..10150
                     /locus_tag="JZ382_001664"
     CDS             9293..10150
                     /locus_tag="JZ382_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAY2427851.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10231..10566)
                     /locus_tag="JZ382_001665"
     CDS             complement(10231..10566)
                     /locus_tag="JZ382_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427852.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10633..12003)
                     /locus_tag="JZ382_001666"
     CDS             complement(10633..12003)
                     /locus_tag="JZ382_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAY2427853.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12081..12617)
                     /locus_tag="JZ382_001667"
     CDS             complement(12081..12617)
                     /locus_tag="JZ382_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2427854.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3748 a         2567 c         2115 g         4204 t
ORIGIN      
        1 gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca ttactttgtg
       61 aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac atattttgaa
      121 aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt ttggaccaaa
      181 caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat ctgtatttta
      241 gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt gacctggttc
      301 ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag tttcaaagac
      361 aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca aactaatctt
      421 gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta aaccagtctt
      481 tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat ggaccaaaaa
      541 ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt tgcttgccaa
      601 aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca cattttcaga
      661 aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat gcttcttcta
      721 tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt ttattcgtga
      781 aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt cttaaagata
      841 aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact ggctgtccga
      901 cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa acatgaataa
      961 gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc cctgtcactt
     1021 ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc gcatttatca
     1081 ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc ttaagatgat
     1141 cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg acgcaattgc
     1201 tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg acttcttgtc
     1261 tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct ttggttatgc
     1321 cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac accatgctgt
     1381 ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc
     1441 agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag tttatcctcc
     1501 attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa
     1561 aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag aaaacaatgt
     1621 aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga
     1681 attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta gtgacgagat
     1741 ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga ttgatcctaa
     1801 tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc tagctgcaat
     1861 caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg aaaaacattt
     1921 agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc aagcggctta
     1981 tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa atgttcaaac
     2041 agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac
     2101 ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat tggagaacac
     2161 tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc cttccggtca
     2221 tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag gacttaatcg
     2281 aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa aggacgagac
     2341 cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta ttctgaaatt
     2401 tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg ttgtacattt
     2461 gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt atgtcggaaa
     2521 tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg gaatccagca
     2581 ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat aaagaattgg
     2641 cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag gttgaacagt
     2701 tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc cattcctgtt
     2761 ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca
     2821 tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg tgctagttca
     2881 gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt tggtcctttt
     2941 tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat ttgcatactt
     3001 cataataata ataacataat acctaacctc tcgtaaaaat actagatttg ttttctttta
     3061 tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc aaacattttt
     3121 gagaagcatt ctcaattaaa ttctgctata gtgtgaaaag ggaggaattt ttatggaaat
     3181 ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg
     3241 aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg gtcaattagc
     3301 aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa taaaagcaag
     3361 tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat tccatttaaa
     3421 agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta caaataaagg
     3481 catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc agccaagaaa
     3541 attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt tcgtgaatga
     3601 tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga
     3661 ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc gtcgcccgga
     3721 atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca acttgcttac
     3781 tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata tccggttaaa
     3841 aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg tcaattatgg
     3901 attctcttct aatttagaac atctttcttc ttggggactt gatagtacca gaaatgccat
     3961 tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg gtgacatctg
     4021 tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac ctactgcagt
     4081 gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc acagcacaag
     4141 cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca tttcaagtta
     4201 aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat agagaaagaa
     4261 aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag aaaaggcaag
     4321 agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg tcatgttttt
     4381 acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg tcaacagcgt
     4441 gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag atatattagc
     4501 agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg aaggccttta
     4561 cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga tcggctttac
     4621 gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa acagccacga
     4681 tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc
     4741 cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat aattcaacaa
     4801 aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt tcaagctatt
     4861 ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa ataagataaa
     4921 gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa
     4981 taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc
     5041 tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat
     5101 caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa
     5161 agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg
     5221 ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt
     5281 gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata
     5341 atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac
     5401 accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca
     5461 ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg
     5521 tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca
     5581 aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca
     5641 tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt
     5701 ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg
     5761 accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa
     5821 tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat
     5881 aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag
     5941 ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat
     6001 ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat
     6061 gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat
     6121 tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg
     6181 ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat
     6241 agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct
     6301 acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt
     6361 cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata
     6421 tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg
     6481 ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct
     6541 tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct
     6601 tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca
     6661 tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc
     6721 cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct
     6781 ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg
     6841 gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca
     6901 acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata
     6961 ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata
     7021 aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca
     7081 aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc
     7141 ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca
     7201 aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt
     7261 ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag
     7321 tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt
     7381 tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc
     7441 gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat
     7501 ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag ataactattt
     7561 atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa
     7621 tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg
     7681 tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga
     7741 tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca
     7801 tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg
     7861 actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta
     7921 agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg
     7981 attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc
     8041 aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct
     8101 acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata
     8161 ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga
     8221 tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa
     8281 aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc
     8341 actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt
     8401 ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa
     8461 gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact
     8521 ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg
     8581 gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg
     8641 tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt
     8701 taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc
     8761 gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag
     8821 attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc
     8881 tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag
     8941 tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat
     9001 ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac
     9061 ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt
     9121 tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt
     9181 taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag
     9241 gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat
     9301 ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag catcagaagc
     9361 ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag attcattatc
     9421 tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca
     9481 cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt
     9541 gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc aagatactat
     9601 tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag atgcgctctt
     9661 agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta tttttctatc
     9721 tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc attcacctgg
     9781 caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa
     9841 tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt actatacaaa
     9901 agatactgta aaagatagta agttgatcaa aagttacata gaactgatca aacaaaatga
     9961 aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct ctgctgcaaa
    10021 aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc aagaacagtc
    10081 tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat tgcttcatga
    10141 acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac cccttatttc
    10201 gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa aatgcattag
    10261 ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag agattttctt
    10321 ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac tatctgaaag
    10381 accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga cgaatttttt
    10441 tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg atacaacgac
    10501 tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt ttgttgatgt
    10561 tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa aaaaataaac
    10621 gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta ctgttccttt
    10681 tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc atccttgatt
    10741 cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt gagtgatcgg
    10801 caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc taggttcaaa
    10861 tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta aaccatcgag
    10921 agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa gtcttccagt
    10981 agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct cttttgtcat
    11041 ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt gtccatgaaa
    11101 agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata tacgtattgt
    11161 tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg attgagtcaa
    11221 aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga tctcttcatt
    11281 ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt ttagcatttt
    11341 tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag attcttctgt
    11401 ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac aaggagcatc
    11461 aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg gtgcatgatt
    11521 tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa tcttcgcacg
    11581 tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag cagctaattg
    11641 ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg gttttgctgc
    11701 agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc cagcttgatg
    11761 gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg gagcgggttg
    11821 taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta gtggattcca
    11881 ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc
    11941 gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt
    12001 cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa ggtggctgat
    12061 ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc caagtattca
    12121 tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa
    12181 tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga cttttcttct
    12241 ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag cgaatccatg
    12301 tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa aaaatctttc
    12361 aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta gttttcaaaa
    12421 taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt gaaatgtgta
    12481 taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt aattgacttc
    12541 attccatatt cttccacgta taccatactt ttgaacagca aatattcttt actataattt
    12601 gttactctcc ccatcattct tacctccgtc tctt
//
LOCUS       DADJYG010000075        12577 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.076, whole genome shotgun sequence.
ACCESSION   DADJYG010000075 DADJYG010000000
VERSION     DADJYG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.076"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1123)
                     /locus_tag="JZ382_001668"
     CDS             complement(<1..1123)
                     /locus_tag="JZ382_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAY2427855.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEIT"
     gene            complement(1245..1841)
                     /locus_tag="JZ382_001669"
     CDS             complement(1245..1841)
                     /locus_tag="JZ382_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY2427856.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2041..4683)
                     /gene="alaS"
                     /locus_tag="JZ382_001670"
     CDS             complement(2041..4683)
                     /gene="alaS"
                     /locus_tag="JZ382_001670"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY2427857.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5071..5502
                     /locus_tag="JZ382_001671"
     CDS             5071..5502
                     /locus_tag="JZ382_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427858.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5547..6896)
                     /locus_tag="JZ382_001672"
     CDS             complement(5547..6896)
                     /locus_tag="JZ382_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY2427859.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6966..8129)
                     /locus_tag="JZ382_001673"
     CDS             complement(6966..8129)
                     /locus_tag="JZ382_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427860.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8258..8581
                     /locus_tag="JZ382_001674"
     CDS             8258..8581
                     /locus_tag="JZ382_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427861.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8764..10113)
                     /locus_tag="JZ382_001675"
     CDS             complement(8764..10113)
                     /locus_tag="JZ382_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2427862.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10182..11135)
                     /locus_tag="JZ382_001676"
     CDS             complement(10182..11135)
                     /locus_tag="JZ382_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY2427863.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11237..12427)
                     /locus_tag="JZ382_001677"
     CDS             complement(11237..12427)
                     /locus_tag="JZ382_001677"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY2427864.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3593 a         2663 c         2192 g         4129 t
ORIGIN      
        1 acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt aagattcttt
       61 ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat tgcagcgttt
      121 ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt tcaagaagaa
      181 tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc attggatcca
      241 agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc agatcaataa
      301 tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact gatgcaaagc
      361 gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata tcatcgcaag
      421 catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt cctaatagtt
      481 cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat gctaaagcaa
      541 aacctatata tccatcaata cgccatccag ttacccattc gattgctgct gaaagcaata
      601 cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc aatgtttcag
      661 aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc aaaaagatgg
      721 aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga tggatgattt
      781 tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc gaaacaaata
      841 acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct gctggctttg
      901 cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat agactgttca
      961 ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc ttagagacaa
     1021 acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt tttgtccgct
     1081 gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt accccttcta
     1141 gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct cctctcttcc
     1201 tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc tggtttcaac
     1261 ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc acctattgct
     1321 tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag agcagagtct
     1381 acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc taacactaag
     1441 actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt tattgattta
     1501 tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag tactctggga
     1561 actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt tggcatggca
     1621 taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc atcttttggg
     1681 cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact aatgaatcct
     1741 aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc taacaagatc
     1801 gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc cctttttatt
     1861 tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg aatccgacca
     1921 tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt ttgtcttcgc
     1981 ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa cgtaaacgcc
     2041 ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac cagcaggatt
     2101 tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta ctttcggtgc
     2161 gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt tcgtcattgc
     2221 cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat ctgaaatatt
     2281 tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca cttttacttg
     2341 tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat caccagcttg
     2401 ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc ttacttgctg
     2461 ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa caattgttgc
     2521 tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt tacttgttac
     2581 agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt tgaagattcc
     2641 gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc agccgccaat
     2701 atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg cacccatgtt
     2761 tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc agattttttc
     2821 gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa agtgggtaaa
     2881 gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat gctcgccaag
     2941 tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga tgatccgatt
     3001 gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg gtgcttttac
     3061 ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa caactgctac
     3121 gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac ccatttctgc
     3181 atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt cattcaaaag
     3241 agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg tgtatccaat
     3301 gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca ttgatttttc
     3361 agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt caaaaccagc
     3421 atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta ccgggaagcc
     3481 atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt tttcgttttt
     3541 tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt cattgaaacg
     3601 ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta cttcaggata
     3661 ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg cttgttcaat
     3721 acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga cataccctct
     3781 cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc gaatatgatc
     3841 tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta cttgtttgct
     3901 tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt tcgttgacgc
     3961 atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt ttttatgagg
     4021 aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta agttccagat
     4081 ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt ctgctaaatc
     4141 gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg ggcctgcacc
     4201 gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc caacttcttc
     4261 atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa gtttttctgg
     4321 atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga tggcttcatt
     4381 tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat ggtgccgtgc
     4441 tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat ttgtgattct
     4501 tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa caccagagtt
     4561 gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt caatcgtatg
     4621 tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac ttaattgttt
     4681 catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca gccaaggacg
     4741 aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt aaactcgata
     4801 acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt gattagtgac
     4861 tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa tcatatcctt
     4921 ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa tttttatttt
     4981 catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg aatcaataca
     5041 tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc ggatgaatgg
     5101 atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca agggattcct
     5161 gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct atttcttgat
     5221 caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg cttcaagccg
     5281 gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca gttgcttgaa
     5341 gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt agctgaaatg
     5401 acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc ttttattgaa
     5461 gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga gattgtgaca
     5521 cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt tttacgagca
     5581 gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc tgcaattgcc
     5641 caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt cacaagacca
     5701 atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct attacgatcg
     5761 tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac gcccaatttt
     5821 tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc cgttcctttt
     5881 aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc ttctggaatt
     5941 tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc taaatcagtc
     6001 gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt tctctctctt
     6061 ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa gtaatcggca
     6121 atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg atgaccaatt
     6181 gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat cagccaattt
     6241 tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg attttccatg
     6301 tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac taaaaactgc
     6361 agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc catatccaat
     6421 gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag cgcttgttca
     6481 ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg ttggtgatgc
     6541 agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac acggatctcc
     6601 ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc tagttcccta
     6661 cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt ttcaaacaaa
     6721 ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat gatatttttt
     6781 ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt gaagcccttt
     6841 gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt attcattcgt
     6901 gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa agcgtttttg
     6961 ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac agtatatgtg
     7021 tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag caagatattt
     7081 aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc actcatctgc
     7141 aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa ccagcttaaa
     7201 ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa agaaattcgt
     7261 tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt ccatccattg
     7321 gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca ccagatatga
     7381 gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac caaattaata
     7441 agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa tgagacagtc
     7501 tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat aaatgtaaat
     7561 aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa atcattcaca
     7621 tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg cgtcaattca
     7681 aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag ccggaaataa
     7741 tatcttaaat aaatgccaaa tggtatgagt aataaaacat taaaagctgg ttccaaaaat
     7801 acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa taccgtgttg
     7861 tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc tgtcatttgg
     7921 gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca caataaataa
     7981 aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata ctttcgatat
     8041 tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac aatcagcgca
     8101 aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt gtatctctct
     8161 tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc tcatttcttt
     8221 caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt actttgtttt
     8281 tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa gaaaaagaac
     8341 tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc tagtcttcaa
     8401 tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac atgctttgtc
     8461 taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt ctggttgctt
     8521 ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca aatcaaagta
     8581 aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc atcgtagaca
     8641 aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc acgtactcct
     8701 catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat tcgaatgttt
     8761 tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca atactatccc
     8821 catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag tccctccaat
     8881 gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa acaaatcagg
     8941 ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa tccaaatgac
     9001 aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc caatagcgat
     9061 gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa gtttagcaaa
     9121 gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac gcattccagt
     9181 aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg tgaccagtct
     9241 tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc tgtttcctgc
     9301 gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat aaacgatcat
     9361 gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct ttttcaattc
     9421 tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg ctagcaagcc
     9481 cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa acagttggaa
     9541 gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag gaataagctt
     9601 gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa aattgatcaa
     9661 caggatcgat acagcagctg taacagctac gggaacaatc attgactggg aaagaccaaa
     9721 tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg ctggaaaata
     9781 aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa tccgttcgat
     9841 atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc ctgttagtcc
     9901 tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac tggcggatcc
     9961 tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca ctgtgcccat
    10021 aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt ctatttttac
    10081 ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat accatagcca
    10141 atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt ctctaccaat
    10201 ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta tttcggcgaa
    10261 aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga ttctgctatt
    10321 gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat tttttgaata
    10381 gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc atttcgatga
    10441 accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc atctggcata
    10501 tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc ttctttgatt
    10561 gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa ggttgcataa
    10621 gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac ataataataa
    10681 tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg acgctgaaga
    10741 gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat tgaacttggc
    10801 gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag cccagtcacg
    10861 ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag agcagccaaa
    10921 tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc gtaatccatt
    10981 acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg tatatctgtc
    11041 gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc tgaaccaatt
    11101 tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga tcttttttac
    11161 tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt taaatagacg
    11221 aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga taaactgcta
    11281 ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg atcctgcctg
    11341 tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt tcgatcagct
    11401 tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta ataggtgttt
    11461 cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa ttagcatttt
    11521 cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa gccaatgcag
    11581 ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt ctagctttca
    11641 accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct atagctttcg
    11701 ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg acggttcctg
    11761 caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc agtccatatt
    11821 tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg gagaaaacac
    11881 catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc tttgcttttt
    11941 tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg gataagcggc
    12001 aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct tcttttgtca
    12061 ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg attgctgctt
    12121 ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc agcgaaccat
    12181 tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca atacatgctt
    12241 ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga ttgatcatct
    12301 tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg atcgtattgt
    12361 ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt agttctttgg
    12421 aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa catatatcgt
    12481 gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact gattatgaga
    12541 attatttaac cacatttttt atttacagta tcttttt
//
LOCUS       DADJYG010000076        12065 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.077, whole genome shotgun sequence.
ACCESSION   DADJYG010000076 DADJYG010000000
VERSION     DADJYG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.077"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            25..267
                     /gene="yaaA"
                     /locus_tag="JZ382_001678"
     CDS             25..267
                     /gene="yaaA"
                     /locus_tag="JZ382_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAY2427865.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            254..1378
                     /gene="recF"
                     /locus_tag="JZ382_001679"
     CDS             254..1378
                     /gene="recF"
                     /locus_tag="JZ382_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY2427866.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1375..3321
                     /gene="gyrB"
                     /locus_tag="JZ382_001680"
     CDS             1375..3321
                     /gene="gyrB"
                     /locus_tag="JZ382_001680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY2427867.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3341..5812
                     /gene="gyrA"
                     /locus_tag="JZ382_001681"
     CDS             3341..5812
                     /gene="gyrA"
                     /locus_tag="JZ382_001681"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAY2427868.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6048..6347
                     /locus_tag="JZ382_001682"
     CDS             6048..6347
                     /locus_tag="JZ382_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY2427869.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6397..6909
                     /gene="ssb"
                     /locus_tag="JZ382_001683"
     CDS             6397..6909
                     /gene="ssb"
                     /locus_tag="JZ382_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY2427870.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6935..7171
                     /gene="rpsR"
                     /locus_tag="JZ382_001684"
     CDS             6935..7171
                     /gene="rpsR"
                     /locus_tag="JZ382_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY2427871.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7314..9287
                     /locus_tag="JZ382_001685"
     CDS             7314..9287
                     /locus_tag="JZ382_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAY2427872.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9293..9745
                     /gene="rplI"
                     /locus_tag="JZ382_001686"
     CDS             9293..9745
                     /gene="rplI"
                     /locus_tag="JZ382_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY2427873.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10019..11386
                     /gene="dnaB"
                     /locus_tag="JZ382_001687"
     CDS             10019..11386
                     /gene="dnaB"
                     /locus_tag="JZ382_001687"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY2427874.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4023 a         2197 c         2661 g         3184 t
ORIGIN      
        1 aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg
       61 acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg
      121 tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa
      181 ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa
      241 ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga
      301 gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa
      361 aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag
      421 tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa
      481 aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat
      541 caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt
      601 tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga agtttttaga
      661 tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat
      721 tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct
      781 ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag
      841 agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca
      901 tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac
      961 gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct
     1021 ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca
     1081 gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa
     1141 attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga
     1201 tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg
     1261 aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt
     1321 tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca
     1381 gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt
     1441 gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag
     1501 ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt
     1561 tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac
     1621 ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc
     1681 tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga
     1741 cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga tgtcaaagta
     1801 tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta
     1861 aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa
     1921 atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt
     1981 gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc
     2041 atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt gaatgcaaac
     2101 aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt
     2161 gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat
     2221 atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt aactcgtgtg
     2281 ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt
     2341 gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc
     2401 gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta
     2461 tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt
     2521 gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg
     2581 agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc
     2641 aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct
     2701 aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat
     2761 gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact
     2821 gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta
     2881 gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt
     2941 ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct
     3001 ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa
     3061 gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt
     3121 tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc
     3181 aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa
     3241 atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat
     3301 gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga
     3361 aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat
     3421 gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg
     3481 ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc
     3541 tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact atgcgatcta
     3601 tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca
     3661 tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg
     3721 gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca
     3781 aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct
     3841 tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa
     3901 cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa
     3961 tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa
     4021 atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt
     4081 tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat
     4141 ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga
     4201 agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat
     4261 ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga catccttgca
     4321 aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt
     4381 gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt
     4441 ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc gtattgcttt
     4501 ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa
     4561 agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat
     4621 gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact
     4681 cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat
     4741 catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt
     4801 actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt
     4861 tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca
     4921 acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa
     4981 tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta
     5041 tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa taccaattat
     5101 caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga
     5161 agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc
     5221 tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga
     5281 cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca
     5341 taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc
     5401 cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa
     5461 ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc
     5521 ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa
     5581 aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac
     5641 agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac
     5701 attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt
     5761 cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat agccatcaag
     5821 actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc ctgctggctt
     5881 ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga
     5941 ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat aactctcctt
     6001 gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga
     6061 aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa
     6121 tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt
     6181 gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg
     6241 ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa
     6301 tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg
     6361 tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt
     6421 ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca
     6481 tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt
     6541 atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga
     6601 acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa
     6661 cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca
     6721 tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt
     6781 ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac
     6841 cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta
     6901 ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg
     6961 taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga
     7021 tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac
     7081 tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat
     7141 gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct
     7201 tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct
     7261 cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa
     7321 ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag
     7381 tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga
     7441 atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg
     7501 aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca
     7561 tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat
     7621 tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt
     7681 ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg
     7741 atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa
     7801 aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag
     7861 atgccattga taaattggat gacaaagaga tttcttattt gaactctttc gtcactacac
     7921 tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt
     7981 ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc
     8041 tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga
     8101 ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg
     8161 ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac
     8221 ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag
     8281 cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc
     8341 acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct
     8401 tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca gatgtagaac
     8461 ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac
     8521 gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt
     8581 ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc
     8641 atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt
     8701 cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct
     8761 tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca
     8821 atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag
     8881 acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca
     8941 atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg
     9001 aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca
     9061 atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca
     9121 gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact
     9181 tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat
     9241 ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt
     9301 aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc
     9361 aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag
     9421 tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga
     9481 agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc
     9541 gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg
     9601 gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat
     9661 ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat
     9721 caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata
     9781 accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc
     9841 aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc
     9901 ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg
     9961 atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat
    10021 gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt
    10081 aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa
    10141 agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg
    10201 taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga
    10261 agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa
    10321 tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac
    10381 agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga
    10441 tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc
    10501 tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga
    10561 agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca
    10621 gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt
    10681 gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga
    10741 aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag
    10801 ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg
    10861 gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat
    10921 ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga
    10981 ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat
    11041 ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca
    11101 gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga
    11161 atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta
    11221 tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga
    11281 agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa
    11341 agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga
    11401 gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact
    11461 agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc
    11521 tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa
    11581 aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta
    11641 cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc
    11701 atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg
    11761 ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc
    11821 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag
    11881 ctttttcaca acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga
    11941 aatttcacaa aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac
    12001 cacttctgtt acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa
    12061 gcgtt
//
LOCUS       DADJYG010000077        11974 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.078, whole genome shotgun sequence.
ACCESSION   DADJYG010000077 DADJYG010000000
VERSION     DADJYG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.078"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(221..1423)
                     /locus_tag="JZ382_001688"
     CDS             complement(221..1423)
                     /locus_tag="JZ382_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2427875.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="JZ382_001689"
     CDS             complement(1426..1620)
                     /locus_tag="JZ382_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAY2427876.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="JZ382_001690"
     CDS             complement(1667..2515)
                     /locus_tag="JZ382_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAY2427877.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="JZ382_001691"
     CDS             complement(2720..3070)
                     /locus_tag="JZ382_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427878.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="JZ382_001692"
     CDS             3665..4111
                     /locus_tag="JZ382_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAY2427879.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="JZ382_001693"
     CDS             4735..5727
                     /locus_tag="JZ382_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2427880.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="JZ382_001694"
     CDS             5743..6627
                     /locus_tag="JZ382_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2427881.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="JZ382_001695"
     CDS             6676..8283
                     /locus_tag="JZ382_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2427882.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="JZ382_001696"
     CDS             8284..9768
                     /locus_tag="JZ382_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY2427883.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="JZ382_001697"
     CDS             9945..10937
                     /locus_tag="JZ382_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2427884.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="JZ382_001698"
     CDS             10989..11858
                     /locus_tag="JZ382_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2427885.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         3708 a         1959 c         2483 g         3824 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttct
//
LOCUS       DADJYG010000078        11902 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.079, whole genome shotgun sequence.
ACCESSION   DADJYG010000078 DADJYG010000000
VERSION     DADJYG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.079"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="JZ382_001699"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="JZ382_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY2427886.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="JZ382_001700"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="JZ382_001700"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY2427887.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="JZ382_001701"
     CDS             complement(2169..2906)
                     /locus_tag="JZ382_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY2427888.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="JZ382_001702"
     CDS             complement(2997..3524)
                     /locus_tag="JZ382_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAY2427889.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="JZ382_001703"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="JZ382_001703"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY2427890.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="JZ382_001704"
     CDS             complement(4401..4886)
                     /locus_tag="JZ382_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY2427891.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="JZ382_001705"
     CDS             complement(5039..5236)
                     /locus_tag="JZ382_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427892.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="JZ382_001706"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="JZ382_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAY2427893.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="JZ382_001707"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="JZ382_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAY2427894.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="JZ382_001708"
     CDS             9211..9423
                     /locus_tag="JZ382_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAY2427895.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="JZ382_001709"
     CDS             9425..11104
                     /locus_tag="JZ382_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAY2427896.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="JZ382_001710"
     CDS             complement(11358..11558)
                     /locus_tag="JZ382_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY2427897.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="JZ382_001711"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="JZ382_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3447 a         2571 c         2067 g         3817 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tc
//
LOCUS       DADJYG010000079        11838 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.080, whole genome shotgun sequence.
ACCESSION   DADJYG010000079 DADJYG010000000
VERSION     DADJYG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.080"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(83..823)
                     /gene="truA"
                     /locus_tag="JZ382_001712"
     CDS             complement(83..823)
                     /gene="truA"
                     /locus_tag="JZ382_001712"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY2427898.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1014..1649)
                     /locus_tag="JZ382_001713"
     CDS             complement(1014..1649)
                     /locus_tag="JZ382_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAY2427899.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="JZ382_001714"
     CDS             complement(1796..3037)
                     /gene="sstT"
                     /locus_tag="JZ382_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY2427900.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3235..4233
                     /locus_tag="JZ382_001715"
     CDS             3235..4233
                     /locus_tag="JZ382_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427901.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4246..6264
                     /locus_tag="JZ382_001716"
     CDS             4246..6264
                     /locus_tag="JZ382_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY2427902.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6324..7040)
                     /locus_tag="JZ382_001717"
     CDS             complement(6324..7040)
                     /locus_tag="JZ382_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAY2427903.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7267..7809)
                     /locus_tag="JZ382_001718"
     CDS             complement(7267..7809)
                     /locus_tag="JZ382_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY2427904.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7814..8041)
                     /locus_tag="JZ382_001719"
     CDS             complement(7814..8041)
                     /locus_tag="JZ382_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAY2427905.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8120..10000)
                     /locus_tag="JZ382_001720"
     CDS             complement(8120..10000)
                     /locus_tag="JZ382_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAY2427906.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10189..10803)
                     /locus_tag="JZ382_001721"
     CDS             complement(10189..10803)
                     /locus_tag="JZ382_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2427907.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10945..11505
                     /locus_tag="JZ382_001722"
     CDS             10945..11505
                     /locus_tag="JZ382_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2427908.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT         3696 a         2362 c         1902 g         3878 t
ORIGIN      
        1 atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt
       61 ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg
      121 cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt
      181 ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt
      241 tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag
      301 ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa
      361 tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact
      421 cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa
      481 cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt
      541 cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt
      601 ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc
      661 cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata
      721 ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat
      781 atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct
      841 gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg
      901 cttctctaag acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa
      961 aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt
     1021 ttgagatgca agatactcaa cctggcaatg ggcacataat taaagaaaaa actgattctt
     1081 tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt
     1141 ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg
     1201 tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca
     1261 ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca
     1321 tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg
     1381 gctgctgtaa ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca
     1441 ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt
     1501 acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga
     1561 taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag
     1621 aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt
     1681 ttaattgtta ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca
     1741 tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac
     1801 gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg
     1861 cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca
     1921 ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat
     1981 tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac
     2041 ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag
     2101 gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg
     2161 caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca
     2221 atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa
     2281 agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt
     2341 taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga
     2401 tcaaaacaag taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg
     2461 attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt
     2521 gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa
     2581 aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag
     2641 attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt
     2701 gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta
     2761 caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct
     2821 tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa
     2881 ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa
     2941 atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac
     3001 gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta
     3061 acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac
     3121 aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt tgttttttga
     3181 gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt
     3241 ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc
     3301 ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca
     3361 gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa
     3421 acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa
     3481 atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc
     3541 acactagttt gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat
     3601 tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg
     3661 cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt
     3721 aaatatctac tattattttt aacaattgga ttagttggct gctctactct atttaataaa
     3781 gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt atttttaatg
     3841 ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta
     3901 tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc
     3961 atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg
     4021 tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa
     4081 ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct
     4141 ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca
     4201 tggctgataa atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt
     4261 atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct
     4321 tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt
     4381 cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc
     4441 gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact
     4501 cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta
     4561 ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg
     4621 ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat
     4681 ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc
     4741 gtgggcggat tgatccttct cctattattg attactagta ttattgtaca acgtagacta
     4801 caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta
     4861 ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg
     4921 tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac
     4981 ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc
     5041 tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc
     5101 aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc
     5161 ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc
     5221 aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga
     5281 acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg
     5341 ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat
     5401 ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa
     5461 gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga taaattaact
     5521 tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc
     5581 ttgaaagctt tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa
     5641 aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga aggaactgct
     5701 gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat
     5761 acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt
     5821 ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta
     5881 gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct
     5941 acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg
     6001 aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca
     6061 ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt
     6121 acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta
     6181 gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg
     6241 gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat
     6301 gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca
     6361 tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga
     6421 ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc
     6481 tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct
     6541 tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc
     6601 cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga
     6661 ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct
     6721 aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa
     6781 gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt
     6841 ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg
     6901 ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat
     6961 tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt
     7021 actaaaatcc tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta
     7081 tcaaataagc aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt
     7141 ctaaaaactg agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta
     7201 ttttaatctt cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat
     7261 agacttttag tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag
     7321 tctttgtact tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg
     7381 caagccatat ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg
     7441 gaaatcaaaa tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg
     7501 accatgttcg taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc
     7561 attttcaatc actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata
     7621 ttctgaaaaa agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg
     7681 gatcaatttc atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc
     7741 agttggttta tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac
     7801 tatattcatt gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt
     7861 ccacaactgc agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt
     7921 tgctcgcatt aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt
     7981 cgattttttg catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca
     8041 tcgtagttct tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa
     8101 aattgattct tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata
     8161 aacgcatcgc gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag
     8221 ccatgtaaat aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa
     8281 tcactacatt ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca
     8341 acgattcaaa attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc
     8401 ctgatcccat agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat
     8461 ctccaataaa agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt
     8521 cgtctggtaa taattcagca tggacttcat caatgcctaa catatcagct acataatcag
     8581 cagtcatctg attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact
     8641 tagctaattg ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga
     8701 tttttttgtc gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat
     8761 cttgtacttg ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg
     8821 cacaaactgt atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat
     8881 ctttgactgc taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc
     8941 gccccagagg atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt
     9001 catcctctgt atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag
     9061 ttttatcaaa gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga
     9121 ctaagatgcc ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa
     9181 tgactaaagc gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac
     9241 taatcagacc aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc
     9301 gatcaatgaa tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa
     9361 ttttggcaaa ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg
     9421 ctaaaatcgt tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac
     9481 cagtcactgc ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa
     9541 tagctccacc agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt
     9601 cttcagtcgt tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag
     9661 ccttgattga ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa
     9721 acaagaatgt aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag
     9781 caatagtaac gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga
     9841 tcataatagg tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga
     9901 caaaaccagt aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga
     9961 tatgatttcg atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt
    10021 cattcttacg agactaagta tagatactta tccatcataa aaaattgacc actatcaagt
    10081 ttagtcaaaa aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag
    10141 actcactaaa agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat
    10201 tttcctgtac agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat
    10261 tttttccttt agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc
    10321 tgtaaaaagg aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt
    10381 accgatgcct tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg
    10441 cgaatgcttc tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt
    10501 actttcttta gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc
    10561 aaaagaacga tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc
    10621 gtcctctgca tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata
    10681 tcttccgtaa agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc
    10741 aacagcaaaa tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt
    10801 catcatttcg cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt
    10861 gtctactatc tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga
    10921 tatgtcggat aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa
    10981 aaagctttgg ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact
    11041 atacgcgaac taacagaaac ggcagatgta catcgttcga ctttttatac acattataag
    11101 gatatctatg atctatatga tcaattagaa agcaactttt ttcgggatct aaatgaaatt
    11161 ctctcttatg atccagccca tagctatgag gaactataca ctcgcttgat tgactatgtt
    11221 tacgcaaata cttttttata tcgcttattt atcctcaacc atggtgatat ttcgtttcag
    11281 aagaaagtta aacaagttat tgaagaaaac tatctgaaca tatggctata tgaagacaag
    11341 aaaacgatta tcacagaaga aatgagattc tttactacgt accacgtcca aggttgtcta
    11401 tccgttctca atcggtgggt agaaagcaat tacaagtact ccaaaaaaga attattagaa
    11461 ctcttacaga aactgaatga tcacatagag aaaatcatgc cttgaataaa aacatactaa
    11521 gcttagttaa gaagaaccgt aaccttctcg tataacaact gaatcttatt tagccaaatt
    11581 gtttactgaa gattttttga gtaaaatttc tataattcct acttattgaa aagtagtttg
    11641 tagtgctgct tctccctctc taagtgatat atcttgttgt accaatcaaa ttcacctcat
    11701 cattaataaa ttacttttag gctaatttat tacaacttat aggttgaagc tgtaaccaat
    11761 ttatagtcat attatatttt tatcatccag tttattgaat tcttctcgtt tacctcaatt
    11821 cattttcaag gggaatca
//
LOCUS       DADJYG010000080        11654 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.081, whole genome shotgun sequence.
ACCESSION   DADJYG010000080 DADJYG010000000
VERSION     DADJYG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.081"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..160
                     /locus_tag="JZ382_001723"
     CDS             <1..160
                     /locus_tag="JZ382_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2427909.1"
                     /translation="VCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTVITE
                     PDAKTQSR"
     gene            complement(220..573)
                     /locus_tag="JZ382_001724"
     CDS             complement(220..573)
                     /locus_tag="JZ382_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2427910.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSIM
                     ESLASGINEARNIED"
     gene            1078..1500
                     /locus_tag="JZ382_001725"
     CDS             1078..1500
                     /locus_tag="JZ382_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY2427911.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            1497..1727
                     /locus_tag="JZ382_001726"
     CDS             1497..1727
                     /locus_tag="JZ382_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2427912.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            2188..2391
                     /locus_tag="JZ382_001727"
     CDS             2188..2391
                     /locus_tag="JZ382_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY2427913.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            2473..3690
                     /locus_tag="JZ382_001728"
     CDS             2473..3690
                     /locus_tag="JZ382_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2427914.1"
                     /translation="MSEKRRDNRGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            3922..4248
                     /locus_tag="JZ382_001729"
     CDS             3922..4248
                     /locus_tag="JZ382_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAY2427915.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            4316..5029
                     /locus_tag="JZ382_001730"
     CDS             4316..5029
                     /locus_tag="JZ382_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAY2427916.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            5339..6190
                     /locus_tag="JZ382_001731"
     CDS             5339..6190
                     /locus_tag="JZ382_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2427917.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            6311..8176
                     /locus_tag="JZ382_001732"
     CDS             6311..8176
                     /locus_tag="JZ382_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2427918.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            8224..9633
                     /locus_tag="JZ382_001733"
     CDS             8224..9633
                     /locus_tag="JZ382_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAY2427919.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            9741..10442
                     /locus_tag="JZ382_001734"
     CDS             9741..10442
                     /locus_tag="JZ382_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2427920.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            10601..11080
                     /locus_tag="JZ382_001735"
                     /pseudo
     CDS             10601..11080
                     /locus_tag="JZ382_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         3937 a         1932 c         2502 g         3283 t
ORIGIN      
        1 tgtatgtgga aataaaacac gattaaagat aagggaagat actgaattaa aaaaattccc
       61 cctctattgt ccgaaatgca gacaagaaaa tttaattgaa ataaagcagt tcaaagtaac
      121 tgtgattaca gagccagacg caaagacgca gagccgataa aatgagatta atacaatctc
      181 attttatcgg ctctttccgt tatgtatgga ttcttttaat tagtcttcga tgtttcttgc
      241 ttcgttgata ccgctggcta aagattccat tatggatagt tctttgtctg taaagctatc
      301 catgtatttc tctatctgta atcgtcgggt gctttttacc aagttattag caggtaagaa
      361 aaattcatca acggaaacat gaagtaacga tacaaggtca taaagaactt gtatgctggg
      421 gtgttgccct ttattttcaa tattagttaa gtaccgtggg tcaatttcaa tcaatgctcc
      481 cacttgttca cgagttaaac ctcgtttcaa tcgagcttct ttaatggcta aaccaaaggc
      541 tctaaaatca tatttatctt cttttttacg catagtagac cacctctata cattttattg
      601 ttcctactga attaaaaaca ggtatagaaa aacgtgttat atggtttata ggtttatatt
      661 taataaaaag cactactaaa cgccaataaa aaaaaccgtt atatggtagt gctatttacg
      721 ctgttaaaat attgtatatt acttccaaat ggcggtttgt tggaggtcaa cgtcgccatg
      781 aagtacatca tatacaataa atttccttac attgggttct tgtcaaaaaa agtcgtctat
      841 ctgcaataga taagtacgtc caccaatgtg gttttataaa tcatatagat agaataacag
      901 aagcatgtaa acagagaaat aaatctgttt atatgctttt ttggctattc agaacttttt
      961 tacaaagttt atttatcagt aatgcaacaa atcccccttt cacattggga ctaagagtga
     1021 aaggagataa acgagcaagg ctcacttcct ttcctagaca gaaagggggt gagaaacatg
     1081 aaaccatctt cttttcagac cacaatagaa aatcagtttg actatatctg taaacgtgct
     1141 atggaagacg agcgaaagaa ttatatgctt tatctttcaa ggattgcaaa gcgtgaggtg
     1201 tccttttcgg atgttggcga ttatcttgtt agccagtttg cgacaacaga taactattca
     1261 actgactttc agatttttac actcaatggg ttatcagtag gcgttgaaaa tgatttgttg
     1321 agtgaagcat tacgtgagtt gccagacaag aaacgtgaaa ttctactgct gttttacttt
     1381 atggacatga gcgattcaga aattgcagac ctgttgaaat tgaaccgttc tactgtctat
     1441 cggcatagaa ccagtggact agccttaatt aaaaagttta tggaggaatt tgaagaatga
     1501 aaacacaata tcctatgatt ccctttcctc tcattgtaaa ggcaacagat ggcgataccg
     1561 aagcgattaa ccagattcta catcattaca gagggtacat aacgaagcgt tccctacgac
     1621 ttatgaaaga tgaatatggc aatcaaagta tggtcgttga tgaagtctta cgtggaagaa
     1681 tggaaaccag actgattaca aagattttgt catttgaaat taagtaatat cctctctcct
     1741 ttcgtggaag cgtgctaaac cattccacgc ttcccgaaca gggaggtttg ttattccacc
     1801 aaagcatatt gagctttcaa tgtgttttga taggctaacg agccattgtt ctttgaaaac
     1861 tgaataaaag taatcgaata cgtttcgata agaaaagagc caacggaact aaccgccatg
     1921 acctatctta taaagatagc gagcgattca tgttagtgat ccgagaagca atctttagca
     1981 ggattgcctg caacgacatt cttatcgtga taatgatact cccatacagt caatagtccg
     2041 agcgtgataa aaccgtcgca ggcaatgagt atggctacat gagaaccatg caggggtgga
     2101 actcccgtga gctttgctaa agctgttcga ttgctggtaa aacaactttt atgaaatcca
     2161 aataagtgat ttggaaagga ggattttatg aagcagactg acattcctat ttgggaacgt
     2221 tataccctaa ccattgaaga agcgtcaaaa tattttcgta ttggcgaaaa caagctacga
     2281 cgcttggcag aggaaaataa aaatgcaaat tggctgatta tgaatggcaa tcgtattcag
     2341 attaaacgaa aacaatttga aaaaattata gatacattgg acgcaatcta gcgtcgccaa
     2401 agggtcttgt atatgataaa atagtattaa gtcgtatcaa ggctctttcc ataaaggaaa
     2461 ggagcaaatg ccatgtcaga aaaaagacgt gacaatagag gtcgaatctt aaagactgga
     2521 gagagccaac gaaaagacgg aagatactta tacaaatata tagattcatt tggagaaccg
     2581 caatttgttt actcgtggaa acttgtggct acagaccgag taccagcagg aaagcgtgat
     2641 tgtatctcac ttagagagaa aatcgcagag ttacagaaag acattcatga tggtattgat
     2701 gttgtaggaa agaaaatgac actctgccag ctttacgcaa aacagaacgc tcaaagacca
     2761 aaggttagaa aaaacactga aactggacgc aaatatctta tggatatttt gaagaaagac
     2821 aagttaggtg taagaagcat tgacagtatt aagccatcag acgctaaaga atgggctatt
     2881 agaatgagtg aaaatggtta tgcttatcaa accatcaata actacaaacg ttctttaaag
     2941 gcttcattct atattgctat acaagatgat tgtgttcgga agaatccatt tgactttcaa
     3001 ctgaaagcag ttcttgatga tgatactgtc cctaagaccg tactaacaga agaacaggaa
     3061 gaaaaactgt tagcctttgc aaaagctgat aaaacctaca gcaaaaatta tgatgaaatt
     3121 ctgatactct taaaaacagg tcttcgtatt tcagagtttg gtggtttgac acttccagat
     3181 ttagattttg agaatcgtct tgtcaatata gaccatcagc tattgagaga tactgaaatt
     3241 gggtactaca ttgaaacacc aaagaccaaa agtggcgaac gtcaagttcc tatggttgaa
     3301 gaagcctatc aagcatttaa gcgagtgtta gcgaatcgaa agaatgataa gcgtgttgag
     3361 attgatggat atagtgattt cctctttctt aatagaaaga actatccaaa agtggcaagt
     3421 gattacaacg gcatgatgaa aggtcttgtt aagaaataca ataagtataa cgaggataaa
     3481 ttgccacaca tcactccaca tagtttgcga catacattct gtaccaacta tgcaaatgca
     3541 ggaatgaatc caaaggcatt acagtacatt atgggacatg ctaatatagc catgacgctg
     3601 aactattacg cacatgcaac attcgattct gcaatggcag aaatgaaacg cttgaataaa
     3661 gagaagcaac aggagcgtct tgttgcttag tagtacaaat gaatttacta cttatttacc
     3721 acttctgaca gctaagacat gaggaaatat gcaaagaaac gtgaagtatc ttcctacagt
     3781 aaaaatactc gaaagcacat agaataaggc tttacgagca tttaagaaaa tataaaaaga
     3841 taattagaaa tttatacttt gttttatgta aaaaaatggt atgattaatc aagtaagaag
     3901 tgattggaag aaggcaagca aatgaatagt atggaaaaag tcaaccagtt tagagatgaa
     3961 cgtaattggc gacaatttca taacgaaaaa gatttggcta tctctatttc tttggaagca
     4021 tctgaattgt tagaactttt tcaatggaaa acgccggaag aagtaaaaga aacacaatta
     4081 gaacgaatca aagaagaact ggcagatgta ttgatctatt catacatgat ggcagacaac
     4141 cttcacctgg atctagatga gatcattgaa gaaaaattga tcaaaaataa tttgaaatat
     4201 cctgtagaaa aaagcgcagg tgtacgaaaa aaatacacag agttgtagaa atgtgtcatt
     4261 cggctgttgg aaagagcaaa agaaaaacag taaaatgagt ctggagggag agaaaatgaa
     4321 aaaaaaagga aaagcaaaaa gatggctagt caatatcctc ttgttcctct tgttattagt
     4381 aggactagct ttgattttca atgaacagat caaggattat tttgtcagag agacaggaga
     4441 taaatatgct atagcgaatg taacaaaaga agatctgaaa aagaataacg ataaagatgt
     4501 cagttttgac tttgatgcgg tagaaccaat gacgacagaa ggggtcatga ggtcgcaaat
     4561 gagaggaaca gatctaccag tgattgctag tatcgcagtc ccttcagttt caatcaattt
     4621 accggttttc aaaggcttgg ataatacgtc actactttac ggagcaggaa cattatctcc
     4681 agatcaagaa atgggaaaag gaaattatgc cttagcaagc catcgtgcaa cgaaccctga
     4741 attgctgttt acaccgctag aaaatttaga gatgggtgcc aagatctatt tgacagattt
     4801 agaaaatgtg tatacataca aaacattttt taaagaaaaa gttgctccta cagataccca
     4861 attactgaat gaagtcgagg gaaaagaaat tgtcacacta attacttgtg gcgacatgga
     4921 cgccgtcacg cgactagttg tacaaggaga actggaaagt gttacatcga taaaagatgc
     4981 gacagatgat atgagatctg ctttcaatct agagacaaaa acattttagg atactgggac
     5041 ataagtcgtt acagattaaa aacagccaaa ggatatgaat cgataaggaa aaaccaattt
     5101 gctgaaaaaa agagaaacgc aaagaggtgc atctctgatt tggttgtttt ctagatagac
     5161 ttgtgttcca gtttttttat tttttatatg aaaaggcttg cataaataaa aattcgatgg
     5221 tatgatgatt ttgaataaga aatggattgt tactgtacgg caggcaaaac cagaattcag
     5281 ctaagaaaca gtaggcgaat tctaggtttt tttatttttc ttgagagggc gaagcaaaat
     5341 gaaaattgat aaaattttga ataacaatgt cgtcatttcg aaaaatggat tcggggaaga
     5401 agtcgtttgt atgggaaaag gactagcttt tcaaaaaaag attggggacg agatttcgcc
     5461 agaagcggtc caaaaagaat ttgttttaag agattctttt gctaaaaacc aattccagca
     5521 gctgatggct gatattccgg cagaggagat cgaatgggtc aaacaagtag tcgaactggc
     5581 tgaagataaa ctaaaagttg aattttcgcc taatatttat ctgacattga ccgatcatct
     5641 tcattatgcg atcacacgag caaaagaaaa tatccaactg cctaatccat tattatttga
     5701 aacaaaaaaa ttttatccga aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa
     5761 acaaaagacg ggtgtggaac tttctgagga tgaagccggt tttattgcct ttcattttgt
     5821 gaatagccaa caaggaaacg aagacatgca agtgacgatg accgctacca caatggtaag
     5881 agatatactg agtatcatca gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa
     5941 ttatcagcgt atcattactc atctgcagtt ttttgcccag cgttatctca aaggggaagg
     6001 atctgacgag caagatgaat ttctctacgc attagttcaa ggaaagtatc caaaagcgtt
     6061 tcattgtgtc gaacggatca atgagtatct tcgacaaacg caaaaacagg aaatggggat
     6121 cgcagagcag atttatttaa cgattcatat tcaaagagta gtaagtgaga aaaaaagcat
     6181 ccaatcataa ggattgttac tgtatggcag gcaaaacctg gattgaaaaa ataggttcac
     6241 gaatgtttgt gactgttttt ttcaatctgg gtttttattt tttaaataaa aaagaaagaa
     6301 ggaaaaaaca atggataatc aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca
     6361 aaagaatgtc aaaagtttag tacattgtgc tacgcggttg cgattcagat taaaagatga
     6421 atctttagca gatacgcaaa agttaaaaga agacccggac gtgatccaag tcgtacaaag
     6481 tggcggtcag tatcaagtag tgattggcag taatgtagca gatgtttatc aatcgatcgt
     6541 ggatgaggaa ggtcttgcag tttcggagga tggaaaagaa gagtccaaaa atattttgaa
     6601 tcgtctgatc gatattatct ctagtatatt tacacctttt ctaggggcga tggcagccgc
     6661 cggggtattg aaaggattct tatcattggc aacggtgatg ggctggctga cagctgatag
     6721 cggtacttat caaatattgt ttgctgcagc agatggtgtc tttaccttct tacctgtcat
     6781 gcttgctttt acagcagcta aaaagttcaa agccaaccag tttttagcag tagcaattgc
     6841 tatggcactt gtgtatccag ctatcacagc agttgctggt gcgggagaag ctatcagttt
     6901 ctttgggatc ccagtgattc tttcaccatc tggttatact tcttctgtca taccaatcat
     6961 tctagccgta tgggttcaaa gtaaattaga gccattcgta aaaaaagtga ttccgcaatt
     7021 tctgcaaatg attttagttc cgcttgtcgt tttagtagtg atggttccat taacatttct
     7081 tgctttagga ccaatcggga cggttgccgg taatgcttta ggcggtctct tcaactcgat
     7141 ttatggattt agtccaatcg tagcaggtct aatcatggga agtttatggc aagtattcgt
     7201 gatgttcggc atgcattggg gattcgtacc gatcatgttt ttgaatatcg aacaatatgg
     7261 gtttgatgta ttgatgccaa tgcttcttcc agccatcttg gcacaaggtg gggctgcttt
     7321 agctgtagca ttgcgaacaa aagatacgaa acttcgagca ttagggattt cttctacagt
     7381 gacttcgtta tttggaatca ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa
     7441 accattcatt gccgcttgta tttctggagg tattggtgga gctattatcg gattttccgg
     7501 agtaaaagct ttctccagca gtttggtcag cttattgacg attccgactt tcattaatac
     7561 ggtggatggt gtagagtcaa atgtgacagt agcagtaata gctacaggaa ttgcttttgt
     7621 tcttgcattc gtaggtacat tgattttagg atttgacgaa caaacacaag acaatcaatt
     7681 agaaaacaaa catgcgaatg caggagaacc gattacttca gcacggcata ccttaaaaag
     7741 tcctttaacg ggtaaagtac tgccattgag tgaagtacca gaccaagttt tttcttctgg
     7801 cgtgatggga aaaggtatcg ctattgatcc agaagtagga gaactagttg cacctgcaga
     7861 cggagagatc acgactattt ttccaactgg acatgctgta gggatcacaa cgactgacgg
     7921 agcggagatt ttgattcata tcgggatgga tacagtagaa ttaaatggga atggctttga
     7981 gatcttagtc aaacaagggg atctcgtcaa agcaggagat ttattgattc gttttgacat
     8041 cgaagcaatt agagcagctg gctatagcgt aatcactcca gttgtcatta cgaatactga
     8101 tgcatttgcc gatatacttg aattagacca aaaagagatt attgcaaacg aagatgttct
     8161 ggcaattgta aaataaggag gaaatcagga tgcaatcacg tttttcagaa aaatttttat
     8221 ggggtggagc aactgctgcc aaatcaatta gaaggggctt atgatcgaga cggaaaagga
     8281 ctatccgttg cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa
     8341 gaatttgact ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat
     8401 cattacgatc gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat
     8461 cgtttttcga ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa
     8521 gcgggattag aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct
     8581 gtgatcacga tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg
     8641 aaaaatcgaa aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac
     8701 agcagtaaag tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca
     8761 gctttaagtc aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa
     8821 gcatggcata atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt
     8881 tcggatatcc aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat
     8941 ccagtcaatc aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat
     9001 gtccaagtac gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc
     9061 gatccggaaa aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg
     9121 gactacatcg gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca
     9181 gcaacttcag aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa
     9241 tggggctggc agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt
     9301 taccagaaac cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag
     9361 gatggcacga ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg
     9421 gctaatgcag tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatcgat
     9481 cttgtcagtg catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta
     9541 gacgatgaag gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa
     9601 gaagtaattg cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac
     9661 acttctcaca tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata
     9721 gtaaagcaaa gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata
     9781 aaattaaaga agatattttc tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa
     9841 gtgaattaga agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc
     9901 tggcatcaga tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc
     9961 gtccttacaa tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat
    10021 atgaagtccg gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt
    10081 tgttttcctt tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc
    10141 agccttatat ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag
    10201 aatttggcaa agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat
    10261 tttctgatga tttctcggca gtatttttaa cagaagaaga acaaggaatt ctgaaaacag
    10321 acgcacagat cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg
    10381 aatattcgga ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat
    10441 aaataaaaaa agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    10501 aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcagatac acggctgttt
    10561 ctggattacc atttattctg gataaggggg tgtggcacga ttttcagaag gaatctatga
    10621 accaatcact gtattgattt tagcttcgat ggtaacccca ttcggcttga cgatcgctta
    10681 tttcttaggg aaaatcattc gtaaaaatat actcaatcgt caagaaatcg atacattgaa
    10741 gaccgctttt cctatgggga tctgtcagat cacagaggga tgtttcccta tcgtattgaa
    10801 cgacttggca cgcaatgtca tagcaacggg agtcggtggg acagtaggtg gcggattaag
    10861 catgttttgg ggcgcagata gccgtattcc ggcatcggga atgtttgcag tcgctacgat
    10921 gaccagacca tgggcattca ttggtgcatt gttagctggt tcttttgtca caggaataac
    10981 cattttatta ttgaaaaaac cagtagatcc gaatgcagaa atcatccaag aagaaaaaga
    11041 ggaagaagac atatcttggg atgatttgac aattagttag gagaggcaga atggattaca
    11101 agaaaaaagt acaatagatg taccttgaga caggaatggt atgaaagaat cagagttaga
    11161 aagaatcgaa tatacagatt ttggtttagg agcgatcgaa actgtccaga gcatcggcac
    11221 cctccttgaa atgacgatga aggaaaaatg agaacctttc gttgtcgaaa gggtctggta
    11281 taccttatgt cgaaggaaag gaaacaaaaa aactttcctg tactattcca aaaaggaaag
    11341 aaagttatta tacagtaaaa aaagaaatta ttcttcatgc tagagatcag tacacgttcg
    11401 agcccaatat caaacactag ttccaggcag gcgcaacagg tgcggttatc actgaatttt
    11461 cttccagcag tgacgattca agtgatattt ttacaaatcc agctatttat cgttaacaaa
    11521 aagattttta ggagaagctg atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg
    11581 gcttttttat ttagagaaac atcatttatc agtgaaacat aacacgagaa caaaatgatt
    11641 ttttcgttaa taaa
//
LOCUS       DADJYG010000081        11406 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.082, whole genome shotgun sequence.
ACCESSION   DADJYG010000081 DADJYG010000000
VERSION     DADJYG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.082"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(87..1442)
                     /gene="celB"
                     /locus_tag="JZ382_001736"
     CDS             complement(87..1442)
                     /gene="celB"
                     /locus_tag="JZ382_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAY2427921.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVFCAIIAFIIWFPFIKFYDNKLLNEEREKAAELAGEKSA
                     N"
     gene            complement(1442..1960)
                     /locus_tag="JZ382_001737"
     CDS             complement(1442..1960)
                     /locus_tag="JZ382_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427922.1"
                     /translation="MQDTVKKKNELEKKQLSLKNMYFNRYLFVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMVLLLAILPAVGEQVKLYRKHQTNVPWTKRYFLFQGVCNILLIP
                     VLFTSGFTLLYPFMANNNRGQLFVFILIVSGIFVSVFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1976..2302)
                     /locus_tag="JZ382_001738"
     CDS             complement(1976..2302)
                     /locus_tag="JZ382_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAY2427923.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2315..4294)
                     /locus_tag="JZ382_001739"
     CDS             complement(2315..4294)
                     /locus_tag="JZ382_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAY2427924.1"
                     /translation="MKQKEKLLLRYLIDHRKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEENGGKILAKPGYGYQLHILQRLTFDLFLSRQEIVPGYARESQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQAIKERLIPYSLKIVSKHGQGIWIEGE
                     ERNRRHFIMDTFFGNHCGNSLKEYLGQSRFFTDISFEELVIIILDETREAKLKVSDFI
                     IQNLSLHLALGIKRLREGFEIKELGIEQEVSDRVEYQVAQRIVQRIEVIADVSFPQEE
                     IAYLTLHLMAKSNHRENKNDQELSKEVVKVLKRLSQEFRFSLIEDHQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFEAVKTHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERATHKLHALIICATGYGSAQLLKNRVISEFGKNITVVNVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVHKIRKVIGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEVLHDLLQKLSINEEEGYIREMRKQIQQRESMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWNQYQGIHFVFLVSPSYIENEGITVV
                     TKAIVRLVDQLNVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4315..4629)
                     /locus_tag="JZ382_001740"
     CDS             complement(4315..4629)
                     /locus_tag="JZ382_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAY2427925.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDLFKQKGEEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLVEVIEE
                     NI"
     gene            4797..5090
                     /locus_tag="JZ382_001741"
     CDS             4797..5090
                     /locus_tag="JZ382_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY2427926.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5274..6365)
                     /locus_tag="JZ382_001742"
     CDS             complement(5274..6365)
                     /locus_tag="JZ382_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY2427927.1"
                     /translation="MNFYQSLQLDPFILKQKIREAASKKEKCMYICSLFLRSLFIVLF
                     AICFIIFITTLFESTHKPYAVVLFCMLMSIRFVDFGYKISHSIISLAIVMLSLLTAPY
                     VHLIKWTVMGMLIHFVLLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFF
                     TQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISFL
                     IVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCALFIGISQFI
                     PFNWVGILGGLALGVCSTYRYKTVFNCFGALAITASLFGVPGAVSIRIFENVLGVCLG
                     VMYIGLTEILIRKIREKHEVNPLVSSTKK"
     gene            complement(6546..8960)
                     /locus_tag="JZ382_001743"
     CDS             complement(6546..8960)
                     /locus_tag="JZ382_001743"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY2427928.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLASYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVQKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWSILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKVALANETVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            complement(9381..11387)
                     /locus_tag="JZ382_001744"
     CDS             complement(9381..11387)
                     /locus_tag="JZ382_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2427929.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTRSYTDATACLTGRYATDPGYASKLNNIITTYGLTQYDTPAS
                     GNAGGGATTGGNNTGSTGSTSGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNTGANTGNTSG
                     SNSSNSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQQLTV
                     SNSGSTNSASSNTGSSANTNNSSSANTGSSSSQGTYTVKAGESVWSVANKNGITMNQL
                     IEWNNIKNNFIYPGQQLIVKGGNSTNTNTGSTTSAAKPNTPNTSATTSTSSSGNTMYT
                     VKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQTTNQQSPAGSKT
                     YTVKSGESVWGVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT         3326 a         2383 c         1981 g         3716 t
ORIGIN      
        1 gagttgcttc tgcttccgcc gtttacacat gtttagagcg tgagacaaaa gtttttcttt
       61 acttttgtcc cacactctct tagaaatcag ttagctgact tttcgccagc taattctgct
      121 gccttttctc tttcttcatt caataattta ttatcataga atttaataaa tgggaaccag
      181 ataatgaatg cgatgattgc acaaaagaca gctagcacag ccgctttcca atcaccccca
      241 ctgccgacaa atgcgccaat tccaactggt gaaggccacg gcatctgtgc aagcatcggt
      301 ttaacgattt ccagccgaat ggcaaaataa gcgagtgtag cagaagccat cggtgctaag
      361 aaaaacggaa ccgctaaata gggattgtaa acgattggca tcccgaaaat aattggttcg
      421 ttgatattga atagtcctgg tacaagagag gcttttccta acactttcag ctgatcagat
      481 ttcgcgagga atgcaataaa gatgatcaat cctaaggtcg cacctgatcc accaacggtt
      541 acataagaat tgttgaattc tcctgccaac ggataatttg cgccttgtgc gttagctgcc
      601 atattagcta atacgattgg ggtcaagaaa gaagtgatga tattcgctcc atggattcct
      661 acgatccaca atgcatggat caagaaataa attaccatga caccaagcca cgtatttgtc
      721 agctgcgtaa caaacccaaa tgggatagca atcattttga agatatccgt tcctagcgca
      781 actaataatc cattgatgat catcaccaca aacgcaatga caaatgttgg gatcagagca
      841 gtgaaagaac gagaaactcc tggcggaaca acatctggca tttttatgac ccaatttctt
      901 ttgacacaca tacaatacaa ttcgaccgca ataatcgaca taataatagc tgtaaaaatt
      961 cctgaagttc ctaaacgttc gacaaaagag cccatccgta tcccatttac tacagtttct
     1021 gtctcattga tagaagcaat caagctgatt ttaccatcag tcatgatcaa ttcagggata
     1081 cacataaaga atgcaaaaac agatagtaat gcccccgtga gcggattaac gttcaacttt
     1141 tgttcatctc gttcaattga cgttaattca taagcaaaca caatggcaaa gtaaatagat
     1201 aaaatcccca tcgtcattgt attgacgatc atgtacaagt cactaacttt gaaaaaagtt
     1261 gcattaaaaa atccttctaa aaatgggaac ggtaatggca aaatattcaa aaccaaaaac
     1321 atcgaaccta caatcgtgaa aggaatagaa gccatccccg ctgccatgac agcccgaacg
     1381 attttaagtt gcgcaatttt ccccataggt cccatcaggt atttctgcaa aaaatcaaac
     1441 attaattatt ctcctttcct aaatacaacg ctttttcata ttgttttata cggatatact
     1501 gctgatcttg attgagcgaa attttcttta aacgatactg gataaataca ctgacaaaaa
     1561 taccgcttac aataagaatg aaaacaaata gctgtcctct attgttattt gccataaaag
     1621 gatacaagag tgtaaatcct gaggtgaaca acactggaat caataatata ttgcagacac
     1681 cctgaaacaa aaaataccgc tttgtccatg gaacattcgt ttggtgcttt cgataaagct
     1741 ttacttgttc tcctactgca ggcaaaatag ctaaaagtag aaccattggt aaactgcttc
     1801 ctaaactttt agctgataga agcagaatca tccattgcat gttcatgaaa aagaaaaagg
     1861 cagtcagata gcgtacgaac agataacgat tgaaatacat gtttttcaaa ctaagttgct
     1921 ttttttctaa ttcgtttttc ttttttactg tatcctgcac aataaatctt cctttctatt
     1981 tatttttttg atacaaataa ttcatttcct gtgcaacttc caataatgtc atcgtcgtca
     2041 tcagatgatc ttgtgcatgg accatgataa tttccatttc tatttttgtc ccactagcat
     2101 attctttcag aagttgagtt tgtgattgat gcgcatgaag aatctcttca tttgcttgtc
     2161 gcaacttctc ttctacttct tcaaacatct ccttacgcat cgagcgaaat gcttcatgta
     2221 tcagtgttcg tgcatttccg ctatgaaaaa tgatatcgaa agacgtcact tgtagcgttt
     2281 cactgtttat tttttcagcc acctgttatc ctccttattc gatcagtttg ataaatttac
     2341 tgctgaaatt ctcaaaaata ggttcagcta agatttcctg ttgcacgttt agctggtcaa
     2401 ctaacctaac gattgctttg gtcacgaccg taattccttc attttcgata taagaaggcg
     2461 aaactagaaa aacaaaatgg attccttgat actgattcca ttgcatccct gaaggaatca
     2521 atgcaacggc tacctttgtt tcgattccaa ctggaacagc gggatgtggg actgccactg
     2581 tatcactgaa tatgatttgt cccatacttt cacgttgctg gatctgcttt cgcatttctc
     2641 ttatatatcc ttcttcttca tttatgctca gcttttgcaa caaatcatga agaacttctt
     2701 ctttttttgg ttcgtacgta taagtcttga aaaatttttc agacagctgt tcggtgtaaa
     2761 tctgcttttt ttgctgtaga gaaaatgctg gtatcggtcg atctgaggaa tgataaggta
     2821 tggattcacc aattaccttt cgtattttat gaacatcatc ttcattcaaa aaaatactga
     2881 catgtaaaac tggaatttta aaaaacattg tcgaaagatc aatagaagaa ataatcaggt
     2941 cgattccttt taaagttgtt tcattgatct cataatagcc ttttacattt accactgtaa
     3001 tattttttcc aaattcgctg atcacacgat tctttaataa ttgagcactg ccataacctg
     3061 ttgcgcagat gatcaacgca tgtaacttat gcgtagctct ttctttttcc aatgccgcca
     3121 tgagatgcaa agccaaatag gcccattcat cttcattgat ggtatagttt ttcaaagccg
     3181 gcatgttgct gaaatgtgtt tttactgctt caaaaacttt gggattttct ttctttatct
     3241 cttcagtcag agggttctca atgcgaatgc ctcggctcaa tcgaatcatc attggtgaca
     3301 aatgatcaag cagtccattg aaaagctggt gatcctctat taatgaaaaa cgaaattcct
     3361 gagacaatcg tttcaaaact ttcactactt cctttgatag ttcctgatca tttttatttt
     3421 ctctatgatt tgacttagcc ataaggtgta aagtcaaata agcaatttct tcttggggaa
     3481 acgatacatc cgcgattacc tcaatacgtt gaacaatgcg ttgcgccact tggtactcta
     3541 ctcgatcaga aacttcttgt tcgataccta gttccttgat ttcaaaccct tctcgtagac
     3601 gtttgattcc caaagccaag tgtaaggaca aattctgtat gataaaatca gaaactttta
     3661 acttagcttc tcttgtttca tcgagaatga tgatcactag ctcttcaaaa ctgatgtctg
     3721 tgaaaaagcg actttgtcct aaatattctt tcaatgaatt tccacaatgg ttcccgaaaa
     3781 aagtatccat gataaaatgc cgtcgatttc tttcttcccc ttcgatccat atcccttgac
     3841 catgcttaga aacaattttt agtgaataag gaatcaaccg ctctttgatt gcttgcatgt
     3901 cctttgtcaa actagaacga ctgatataaa gctcttctgc caaatcatcc ataaaaagaa
     3961 tggcatcttc caaaagcagt tgattcaata tatacttttg tcggtcttcc gactcataaa
     4021 atccttgaga ttctctagca tatcccggta ctatttcttg ccgactcaaa aacagatcaa
     4081 atgtcaatct ctgcaagatg tgcagttggt agccataacc tggtttagca aggatcttac
     4141 ctccgttttc ttctactaac tctttgatcc gctgcaagta attacggatt gtccgatcag
     4201 ataataacaa ttcagaagct aaacgttgac tggtgacata ctccttcctg tgatcaatca
     4261 aatatctcaa cagcaatttt tccttctgtt tcattatcat ccactcctat tccatcatat
     4321 gttctcttcg atcacctcta ctaacttctg gatgcctgta gggattggga catacgcttg
     4381 gaaagggata ctaacaacag gctttccaac agattctcct aattttttga atttatccaa
     4441 aaacatcgtc gtttgcgggc tgatcagaaa taaatcaaaa tcactattct taatcatatt
     4501 atcgccttct gtagctgaaa ctgcatctac gagtacctct tctccttttt gcttaaacaa
     4561 atccgttgtc tttgaggcca tcatcgatga tgacattcct gctgcacaaa taatcaatgc
     4621 ttttttcata aatcaatcac tccttttatt tttataaatc aagcataact tgtaagcgtt
     4681 ttctattcaa aatatatatt tccgtagcat tacggaaaaa atgtgtatca tatcttgaat
     4741 aataatttct ggctccgtga tatactagtg atagaaattt tttaggaggg gatttagtga
     4801 gtatcacagt gaatattttg tatacaagta caaatggaca agccagaaaa tttgcagaag
     4861 aaatggtcga aagaaacatt gtcaaagcga ttcgcgcaga agaaggaaac gagaaatatg
     4921 actattactt tccactagaa gatccagaat ctcttttact gatcgaccgt tggaaagatg
     4981 aagaagcaat cgaaaagcat cacaaatccg aaatgatggc ccaaataaac gaactccgta
     5041 aaaagtataa attaaaaatg aaagtcgaac gtttcactga tctaccttaa aacaatgaag
     5101 ttgtgacaga catggtcagt ttcaagaaac aagaaggaat ttacgaaaat tgctactcaa
     5161 atttttgtaa aatttgggct catttttcga agaagatact tctggaacac cgtttattcg
     5221 taaaaagagg ctgtgacaaa agtcttgtca cagccccttc ttcatttttt tcattatttt
     5281 ttagtggaac ttaccaaagg attcacttca tgtttttctc ttatttttcg gataagtatt
     5341 tcagtcaacc caatatacat gacccctaga cataccccaa gaacattctc aaagatacgg
     5401 attgacactg ctcctggaac cccgaataaa ctggcagtga ttgccaaagc accaaaacaa
     5461 ttaaaaactg ttttatatct ataggtagaa caaacaccta aagctagacc acctaatata
     5521 ccgacccagt taaaaggaat aaattggctg ataccaataa ataaagcaca tccaatcaat
     5581 gacccaatga ttcggtctac tgctcgttct ttgaaaccaa tcgacatcaa tccatagcta
     5641 gatacaatag aagaaaatgc aaaccctgcc cacatcaatc gctgaaaagg gacatactct
     5701 cctgcaacaa tcaaaaagct aatgccaaat gcatagatga gcatccaaat tttttcctgc
     5761 gaatacatgc ctttcaaaaa aatctcttta aacaagcttt tgtctttgtt cttttctcga
     5821 tgttttctgt aaaggagaac tgaaaaccaa caaaaaaaca aaaacaataa gctacttgtt
     5881 tgtgtaaaga aattcttgtt caacgaatct ttaggtaacg aataaacaat aaagagatat
     5941 gaaaagccgt acaaagaagc attccccatt ttgggatcac tagcggttgc cattaatatg
     6001 ctgcttagta aaacaaaatg aatcaacatg cccataactg tccatttaat cagatggaca
     6061 taaggcgctg ttaatagtga gagcataacg atggctaaac taatgatcga atgagaaatc
     6121 ttataaccaa aatcaacaaa acgaatactc atcaacatac agaataaaac caccgcgtat
     6181 ggtttatgcg tcgattcaaa caatgttgta atgaaaataa taaaacaaat ggcaaacaat
     6241 acgatgaata atgatcgtaa aaacaatgaa caaatataca tgcacttttc ttttttagaa
     6301 gctgcttctc gtattttttg ttttaaaata aacggatcta gttgtagaga ctgataaaaa
     6361 ttcacctgtt gtcgtccttt cttttacttt tatttcaata ttttttacaa tcaactatga
     6421 caggttgtaa atactatgga acatcatatg gcgttgttat tttcttgtca actattttct
     6481 ctattcatgt ttttttacaa aaaaacagct gacagagatt ctgccaactg cttttatcag
     6541 gttatttaat tagcaacgat attgactaac ttatttggta ccgcaataac ttttctgact
     6601 gttttgcctt cgatatattc ttgcactgtt tcattggcta atgctacttt ttctagctca
     6661 tctttcccta agtctcgggg aacattgctt ttcgcacgaa ctttaccgtt tacttggaaa
     6721 acaacttcta cttcgtcttc cacaagtgct gcttcgtcgt atgttggcca tggtgcgtaa
     6781 gaaataccac catcatttcc aaggatagac catagttctt cacccatgtg aggagcaatc
     6841 ggtgcgagta gttgaacaaa tccttcgata tattcataag gcagagcgtc cactttatag
     6901 gcttcattga taaagaccat caattgggag attgctgtat tgaaatgaag attttcatag
     6961 tcttctgtca ctttttttac tgtttgatgg tatactttat caagtttgcc gtcgttcaat
     7021 gtagtgatac ggtcacgcat tttattgttt tcatcaacga tcaaacgcca tacacgatct
     7081 aagaatttac ggcttccttc taagccattt tcgctccaag caatcgaagc gtctaatggt
     7141 cccatgaaca tttcgtacat gcgcagtgta tctgcaccgt aagtttcaac cacatcatca
     7201 ggattgacta cattaccgcg agattttgac attttttcgt tgttttcgcc taagatcatt
     7261 ccttgattgt atagtttttg gaatggttct tttgtaggaa caacaccgat atcgtataag
     7321 aacttatgcc agaatcgtgc atatagtaaa tgaagcaccg catgttctgc tccaccaatg
     7381 tagatgtcaa ctggtagcca acgcttcaac ttttcataat ctgctagctc ttttttgtta
     7441 tgagggtcga tataacgcag gtaataccat gagcttcctg cccattgtgg cattgtattc
     7501 gtttcgcgac ggcctttttt acctgttaca ggatcaacga cattgaccca ctcctcaata
     7561 tttgctaatg gagattctcc agtaccgctt ggcttgatat tttttgtctt aggcaaacgt
     7621 aatggtaact cttcttctgg gacagctgtg actgttccgt cttcccagtg gatgattggg
     7681 attggttcgc cccagtaacg ttgacgggag aatagccagt cacgtaagcg atagctgact
     7741 tcttttttcc ctacgccatt ttcttctagc cattcattca ttttgtcgat tgcttcttgt
     7801 ttgttcatac cgtctagaaa ttcagagttg atgtgcgtac catcttctgt gtaagcagct
     7861 ttttgaacat ctccaccttc gataactgga agaatctcta gtccgaaagt ttgagcgaat
     7921 tcaaagtcac gttcatcatg tgctggaaca gccatgatag ctcctgtgcc gtaagaagct
     7981 aatacataat ctgcgatcca gattgggatt tctttgccat tgactggatt gattgcgtaa
     8041 gcaccagtaa atactcctgt tttttctttt gcaagatctg ttctcttcag atctgatttt
     8101 ttcgctgtct cttcgatata agcttctaca gcagcttttt gttcaggggt cgtaatttct
     8161 ctaacaagat ctagttcagg tgcaagaacc gagtaagttg caccgaataa agtatctggt
     8221 cgtgtcgtaa acacggtata ggatttatcc gtaccagcca ctttaaattc aatatttgct
     8281 cccactgaac gaccgatcca atttctttgc atttccttga tgctttctgg ccagtcaact
     8341 agttctagat catccaataa gcgatcggca taagcagtga ttttcaacat ccattggcgc
     8401 attggttttc tgactacgtc atagccacca cgttcacttt ttccatcgat cacttcttca
     8461 tttgaaataa ctgttcctaa ttcaggtacc cagtttaccg ctacttctgc ttcataagcc
     8521 aggccttttt cataaagctt tgtaaagatc cattgtgtcc atttgtaata ctctggatca
     8581 gttgtattca cttcgcgatt ccagtcataa ctaaatccta aagaattgat ttgacgtcgg
     8641 aatgtttcga tatttttttg ggtaaattca gccggatcgt ttcctgtatc taacgcatat
     8701 tgctctgcag gtaggccgaa tgcatcccac cccattggat gtagtacgtt gtatccttga
     8761 cttcttttca cacgggaaag gatatccgtt gccgtataac cttcaggatg ccctacatgg
     8821 agcccttgtc cggatggata gggaaacata tccagtgcat aaaattttgg tttttcagga
     8881 tcatcatggg tgttgaaggt attcgttttt gcccagtatt tttgccattt tttctcaatt
     8941 tctttatgat tgtaactcat tttctgtcct cctgttctat taaaaacgct tgtttctatt
     9001 atagcgaaaa aaccggcaga atctgtttct ttttgttttc ttttactttg ttttctcaaa
     9061 cttcacaaaa gaaataaaaa aatcctataa aaagattgct ctttttatag gacgtaatct
     9121 acgcggtacc acctattttt accttaatga aaaggcctcg aacgttgtaa cggccgtcac
     9181 cgccacctgc ttactcgaat attcagcaag ggcaactcaa aggcgagttc aaaagcatcc
     9241 gtgcacattc tcaccaacca tgtgctctct gaaacttcag tctttttact acttcctttt
     9301 caagtgttgt catatttaag tagccttata gtaacaaata tttttgtgga attcaatcgt
     9361 tatttctttg aacagctatt ttatttagct acaatcaatg tttgacctgg ataaataaag
     9421 ttgtttttga tattgttcca ttcgattagt tgagccattg taataccatg gctgtcagca
     9481 acgccccaaa cagattctcc agatttcaca gtataggttt tcgaaccagc aggtgattgt
     9541 tggtttgttg tttgagacga gcctttcttc acgatcactt gttgtcctgg atagatgaaa
     9601 ttatttttga tattattcca ttcgatcaac tgatccatcg tgatatgatg tttgttcgct
     9661 acgctccaaa ctgactcgcc tgctttcacg gtatacatcg tattgccaga ggatgatgta
     9721 cttgttgtag ctgacgtatt cggtgtattt ggtttcgctg ccgaagtagt tgaacctgta
     9781 ttggtatttg ttgaattgcc gcctttaacg atcaattgct gtcctgggta aataaagttg
     9841 tttttgatat tattccattc gatcagctga ttcatcgtga tgccgttttt atttgctacg
     9901 ctccaaacag attcacccgc cttgactgta taagtgcctt gagacgaact gctgccagta
     9961 ttagctgaac tgctgttatt tgtattggct gacgaacctg tattacttga tgcgctgttt
    10021 gttgacccac tgtttgaaac agtcagttgt tgtcctggat aaataaagtt attttggatg
    10081 ttgttccatt ggatcagttg agccatcgta attccatatt tgttggagac actccaaaca
    10141 gagtctccgg ctttcactgt gtatttcgtt cctgaagccg tattagaaga acttgagtta
    10201 gacgaatttg aaccacttgt gttgccagta tttgctccag tattagaaga accgccattt
    10261 gatacgatca gctgttgtcc tggataaaca aaattattct tgatattgtt ccattcaatc
    10321 aattgagcca tcgtaattcc gtggtcattt gagatcttcc aaacagaatc gccagattga
    10381 acagtatatt tcgccccagt tgtttgtcct ccatttgatg taccagcatt tcctgaagag
    10441 ttggtattgt taccagaatt attcgtcgaa gagccatttt cttggctacc cttgatcgtt
    10501 agtttttgtc ctggataaat gaaattgttt tggatattgt tccattgaat caattggtcc
    10561 attgtaatac cgaatttatt ggagattccc caaacagaat cgccagattg aacagtataa
    10621 gtggtcgaag ttgctgaacc tgagtttgta ttaccagaat tacttgaacc gctagtacta
    10681 cctgtacttc cagtattatt accacctgta gtggctccac cgcccgcgtt tccgctagct
    10741 ggtgtatcat attgggtcaa tccgtaagtc gtgatgatat tatttaattt gcttgcataa
    10801 ccagggtctg ttgcataacg acctgtcaaa caagctgtcg catctgtata ggatctcgtg
    10861 ttgctcttcc aagtaccggc atagtaataa ccgttgccaa atgaagtatt tctaagtaca
    10921 taggcattgt cattgaacga ttcagcaaaa gaaggatatt gtcggaaagg ctccttttta
    10981 tttacccatt gattatttaa aaattcaagc gtatccatgt agacggtttg tccttgatag
    11041 ctgcctttta tcccaaaaag attataataa ggtgcttgcg ataacgcact actgccccaa
    11101 ccactttcta caactgcttg tgcgatcatc actgatgcat acaaatcgtt ggcttgagca
    11161 attggctgcg cataagtagc aatttctgtg ataaatgccg ctgtattgat tcttgcgccg
    11221 aactgaacag gtgtttcatc cgcttgaaca ggaaccggct gtactaatgg agcagcgaca
    11281 gaacagccta ccatcgcagt tcccacgatt gctgcccctc gttttgcttt tcgaaaacgt
    11341 ttagtctgtt ctaaacgtct ccgttctttt cttgcaatgt tttccatctt ctatcctcca
    11401 acttca
//
LOCUS       DADJYG010000082        11168 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.084, whole genome shotgun sequence.
ACCESSION   DADJYG010000082 DADJYG010000000
VERSION     DADJYG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.084"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            130..1389
                     /gene="lysA"
                     /locus_tag="JZ382_001745"
     CDS             130..1389
                     /gene="lysA"
                     /locus_tag="JZ382_001745"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY2427930.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1376..2176
                     /gene="dapF"
                     /locus_tag="JZ382_001746"
     CDS             1376..2176
                     /gene="dapF"
                     /locus_tag="JZ382_001746"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY2427931.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2179..3060
                     /locus_tag="JZ382_001747"
     CDS             2179..3060
                     /locus_tag="JZ382_001747"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY2427932.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3057..3773
                     /locus_tag="JZ382_001748"
     CDS             3057..3773
                     /locus_tag="JZ382_001748"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY2427933.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3779..4474
                     /gene="dapD"
                     /locus_tag="JZ382_001749"
     CDS             3779..4474
                     /gene="dapD"
                     /locus_tag="JZ382_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAY2427934.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4490..5491
                     /locus_tag="JZ382_001750"
     CDS             4490..5491
                     /locus_tag="JZ382_001750"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY2427935.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5488..6849
                     /locus_tag="JZ382_001751"
     CDS             5488..6849
                     /locus_tag="JZ382_001751"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAY2427936.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6809..7960
                     /locus_tag="JZ382_001752"
     CDS             6809..7960
                     /locus_tag="JZ382_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAY2427937.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7935..9104
                     /locus_tag="JZ382_001753"
     CDS             7935..9104
                     /locus_tag="JZ382_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY2427938.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9279..10658
                     /locus_tag="JZ382_001754"
     CDS             9279..10658
                     /locus_tag="JZ382_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY2427939.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10939..11168
                     /locus_tag="JZ382_001755"
                     /pseudo
     CDS             10939..11168
                     /locus_tag="JZ382_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3723 a         1950 c         2498 g         2997 t
ORIGIN      
        1 taaaaaaata tttcgaaata tactagacaa ttcagaaaat tttgtttaat attattccaa
       61 taaaattcaa atctttatca aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg
      121 aatgaaaaaa tgacagaatt gataattggc ggagtcacag caagcacatt ggcagaaaag
      181 tataagacac ctctgtatgt gtatgatgaa gaaaagatga gacaaacgat gctgtcattc
      241 aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc tttacgcctc taaagcattc
      301 caaacgattg agatgctgca tttagttcgt gaatatggat tcgggctaga tgttgtcagt
      361 ggtggggaaa tctataccgc tttgcaagct gattttccta gagatactat ctacttccat
      421 ggtaacaaca agacgccaga ggaactgatc tatgcacttg aaaatgattt gttgcatatt
      481 gttgccgata atttgatgga agttgaatta ttagcagaat taagccaacg ctatttaaaa
      541 aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag cacatactca tgaatatatc
      601 gtcactgcgc atattgattc aaaattcggc atgagctatg aaagtaaaga gtgccaacaa
      661 gcccttcagt tgataaaaga aagcagctat ctagagttag aaggatttca tgctcatatc
      721 gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa tcgacaaatt gacgaattac
      781 ttaaccgatt ttgaccaacc tctttccttg aatcttggcg gagggtttgg gattcgttat
      841 acacaaaatg atcagccgat cccagtcgaa gaggcactaa aaaaactgat tgaatatacg
      901 gaagaagctt tggaaaaacg aaaactgacc attcgtcaat tactgatcga accgggtaga
      961 agtattgttg gagaagctgg tacaacacta tacacgattg gtttcatcaa aaagacacca
     1021 cataaaaaat attactttgt agatggcggg atgacagata atatccgtcc agctctgtat
     1081 caggcagaat acgattgtga tttagcagca aaactgtcgg aagaaaaaac agagaaagtg
     1141 acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg
     1201 aaagcagaat cgggtgatct tttagctgtt tattccacgg gagcttatgg atactcgatg
     1261 agcagcaact acaatcgagc gacacgacca gcagttgttt ttgtcaataa tggccaatca
     1321 agactgattg ttcgcagaca agcattttct gatctactaa ggggggaagt ttcctatgat
     1381 ccttcataaa tataatggct gcggaaatga ttttatctta ttagattacg cagaagacac
     1441 agattatagt caattagccg cttctttatg cacaaaagaa aaatatgata cagacggatt
     1501 gatcgccgta aaaatcgatc cgttggaaat gatctattac aacaaagatg gcagccgcgc
     1561 gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac gtaaatgatc agaacatgat
     1621 gaatgaacag gaattcaacg tagagacatt agcaggcaca agaaaaatta cggtgttgga
     1681 tccctcacct ttttattgca gcgtagatat gggagtgcca gatgattctc cggaaaaaat
     1741 caaagctaat caaacttatc caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt
     1801 aacgatcact agtttatttt taggaacgat tcataccgtt gtttttgttg aaaatgcgcg
     1861 tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat catcctttgt tcaaagaaaa
     1921 aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga
     1981 aagaggagta ggttggacac ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc
     2041 taaggaaact gggaaaattt cagcagatca ggtagttgtt cacttggaac aaggagactt
     2101 gatgatctct ggtgaggatg cgatacaaat ggctggccca gcagttcatg aatggacaag
     2161 aatattggag gaatgaagat gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat
     2221 gaagagggat cagtagatta tgctggtttg gaaaaattga tccaattcca tttagatgaa
     2281 caaacagatg gattattagt tttaggtaca acaggagaat cttctacgct cacacaatct
     2341 gaggaggagc agatccttca actgacagta aagaaagtgg ctggccgtgt gccggtgatt
     2401 gcaggagctg gaacgaataa tacgaaagaa acgattgaaa aagcgaagca ttttgcttct
     2461 ttgggggcag acgcactttt ggtcatcacc ccttactaca acaaaacaag tgatgcagga
     2521 ctagctgctc attttacagc aattgcagaa gcctcaccga ttccgcttat tttgtataat
     2581 gttccttcta gaacaggcat gtcgattcca atccatgtat tagtaaattt agcagaacat
     2641 cctaatatca ttggtttgaa agaagcctca ggagatatgg cttatgtgat ggatgccgct
     2701 cggttgattg gagaagaatt cttcctttac agtggaaatg atgatttgat cctaccagta
     2761 atgagtgtag gaggaagcgg tgtaataagt gtatgggcga atatccaacc taaaatagtc
     2821 catgaattag taaaagatac gcaggacgga agatggcagc aagcaaaaga aaagcaatta
     2881 aatgcacttg aactgatcca tgccttgttc agtgagacca atcctattcc tgtcaaagca
     2941 gccatgtcat tattagactt accttccgga cctctacgcc tgcctttagt ttctctttca
     3001 aaagagaaaa agaaacaatt agctcagctt ttgttaaaag aaaggacggt tgaaaaatga
     3061 aaatcggact gattggcagt ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa
     3121 gaggagatac ggtccttctt ttacggacgg cacctaaaga agcaactacg cttgctttta
     3181 cggaactgcc agaactgctt attgattttt ctcatccaga taatttagag aaaatccttt
     3241 cgtatagctt atcatatcaa cttccgttag tcattggcac gacaggttat acacaatcac
     3301 agttccaaga aataaaagaa catgccaaac atgtacctgt gatgtatagt gctaattttt
     3361 cgttcggcat catggtgatg aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct
     3421 ggcagatcga actgattgaa aaacatcata gtcgtaaaaa agatgcacca tctggtactg
     3481 caaatatgct gcttcaaaca atccaagaag tgcaaaagct gcagcccgta tacaactggc
     3541 aagggaaaaa aagagaagaa aaccaaattg gtgtacacag tattcgtgca ggttctttac
     3601 ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat atttacgatc aaacatgaat
     3661 ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc agcggactgg ttgaaagatc
     3721 agtcaccagg ttattacaaa ttagaagatt tattaatgga caaaggggtg taaaaaacgt
     3781 ggaagctcaa gaaatcatcc gctacattca aacggcatct aaaaagacgc ctgtgaaagt
     3841 ctatctgaat gtaaccgagc caatcgaatt taaagaaagt aaagtttttg gtgaaggaag
     3901 cagcttcgtt gtttttggtg actatgagac gattgctccg gttttagaag ctgaatcaga
     3961 aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac agcgcagttc ctttgctaga
     4021 tattaaaaaa attaatgctc gtatcgaacc aggtgctatt atcagagatc aagtgtcaat
     4081 cggcaacaat gccgtgatca tgatgggagc gattatcaat attggtgcag taatcggtga
     4141 aaacacgatg attgatatgg gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg
     4201 ccatattggt gcaggagctg tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt
     4261 tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg attgttgaag gtgtccatat
     4321 cggaaaagat gcagtcgtgg cagcaggagc agtggtttta gaagatgtgg cagcagaaac
     4381 tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa
     4441 tacagcattg attgctgcgt tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat
     4501 agatacctgt attgtggggg caaccggatt agtcggccaa acgatgctca aagtgttaga
     4561 agaatatgat tttccagtag gacgattgaa attattagct tcaaaacgtt cagccggtaa
     4621 agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa ttgacagaaa cttcatttga
     4681 aggatatgat ttagcgctat tttctgcagg tgcttctgta tccaaagagt ttgcgccaat
     4741 cgcacgaagt cagggactat ttgtcataga taattcctca gcatggcggg aagattcgga
     4801 tattccatta atcgttccag aggtcaatct ggaagactat acattgaatc ggctgattgc
     4861 aaatccaaat tgttcaacca ttcaagcggt acttccgctt aaagctttac aagaagcctt
     4921 tggtatccag cgagtcaatt attcgaccta tcaagcagtt tctggttccg ggcaaaaagg
     4981 cattgaagat cttcatcgta cgcaaaatga cgaggaacca gttttgtatc cttataatat
     5041 tagccaaacg gtgattccgg aaatcgatca gcctttggaa aatgggtata caaaagaaga
     5101 acagaaaatg ataaatgaga cccgtaagat tctgcatcag cctgctttgc cagtatcagc
     5161 aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc tctgtggcag ttcaactgca
     5221 aaaagagttt acgacggaag aaattagaga gtgtctcagc cagtttccag ggattcaatt
     5281 agaagatgat ctagcagcac ataaatatcc gacgagcatt cttgctagag gaacagatat
     5341 ggtttatgtt gggaggatca gaaaagacac ttctttagag aacggattgt tgttctatac
     5401 gactgccgat aacatacgca aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt
     5461 aatgcagaaa atagcggagg tggcaagatg agtaatacgc tgaaagtagc gaaattcggc
     5521 ggaacatcga tgtctcatga agagcaattc aaaaaagtcc gtacaattgt tctttcagat
     5581 cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa
     5641 gtaacagact tactttattt gatttatgct taccttcagc atcatgttga ttggacaccc
     5701 ctttggaaaa ggatttgtga tcgtttcatt caagtaagag atgttcttgc cctgcagtta
     5761 cctattgaag atgaactagc acgcatacag aaacaactgg aagacgaaaa gataactgaa
     5821 gattatttag taagtagagg tgaatatctg actgctcaac tgatggctga gtatcttggt
     5881 taccagttta ttgacgcaaa agacctgatt ttctttgact atgatgggaa aatcgatcag
     5941 gaaaaaacag cacaggaaat cacaaaagca tttaaaagat atccgaaaat ggttgttcca
     6001 gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac
     6061 attaccggcg cgatactggc gaagatgttg caagcggaaa gttatgagaa ttggacggat
     6121 gtttcaggaa tcatgatggc tgatccgcga atcatcgatc atcctaaaat catcgaggaa
     6181 atctctttta gagaactgag agagatggct tatatggggg ctaacgttct tcatgaagca
     6241 gctgttttcc cagttcaaga ggctgatatc cccatccaaa taaaaaatac caatgatcct
     6301 ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa aggaaaatgg actgacagga
     6361 atagctggac gcaaggattt tctttccatc actttattca aacgtcatat gtccgatgaa
     6421 atcgggttta tctggaaagc aatgagcatt tttgctaaac atgagatatc aatcgaacat
     6481 attccttcag ggatcgacaa tatcggagtg atcgtctcag cagaagcgat tgccgataag
     6541 ttatttctca tcacgaaaga attaaaagaa acacttggcg tggaagaaat cgtcgtgatc
     6601 gaagacttag ctttgatctc tgtcgttggg ggtccgcata aagaattgat tggattatct
     6661 gggaaagtgt tatcgatatt gaacaaatta gaaattcgca catcgatctt atcacaagga
     6721 gcacaggagt tgaatctgat catcggtgta ccgaataatc aatatgaaac agtagtgaag
     6781 ggtatctatg aagggatggt gaatacggat gctgaaagaa tatcgaatgg cactccatca
     6841 aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa
     6901 aaacagcggg gccgttatcc atgaaatcgg cgaaacagga ctactccttt attttgataa
     6961 tcagcaagaa gaaacgattg cttttcgaac agatatcgat gcactgccag tcaaagaaga
     7021 aacgaatctc tcttttgctt ctaagcatga aggttatatg catgcatgtg gtcatgacgg
     7081 acatatggcg atgcttttag ggttgactga ctttataact aaacacattg aggatcttat
     7141 ttataatgta gtactgatct atcagccatc agaagaaatt tccggtggcg cacaaagcgt
     7201 tattcagtca ggattgttgg ataaatatga agtacaagca atatttggct ttcatctatg
     7261 gcctggacta gctaagggag aagttttctc tcgaccagga cctttgatgg cacaaagcag
     7321 tgaaacagac attatcatta aaggccagtc tgcacatatt gcttctagtg aaaaaggaat
     7381 cgatagtctg gaggcagctg tacgattttt gaagaatgtt tatgattttg accggcaatt
     7441 accggcagag tggaatcatc taatgaaatt tggccaaatc caaggtggaa cgatccgaaa
     7501 tgttttagct ggagaagtta ttgtatccgg aagtatccgt tcttacagtt cagatatgca
     7561 aaattatttg aagaggggat tagaccattt agcagaacag tttcactcta cagctactat
     7621 ttcttttcgc tataatgacg gctatccagc tgtaatcaac catagaggct tatatgcagc
     7681 actgttagat tcaaacaaac tacatgaatt agatgctcca gttcttcaag ccgaagattt
     7741 tggcatatat acgcaacaat atccatgtgt atttttcttt ttaggtgtcg gtgatacccc
     7801 tgctttacat gatccgacgt tcgattttga tatggatgtt ttagaaaatg gtgtagaatg
     7861 gtacaaaacg attcttttta cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga
     7921 ggagcgagta aataatgatg aatttatcca atcgatttag tcctaggctt tctagaatag
     7981 aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc gataccagat gtgatcaaac
     8041 tcaccctggg agaaccggat tttccaactc ctgaacatgt caagcaagca ggaatagcag
     8101 cgattgaaga agacttttct cactataccg gaatgagagg attagaggaa ctgcgggaag
     8161 cggcttgtat atttcagcaa cagcgttatg gattaactta tgatccgcaa acggaagttt
     8221 tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt gttatctgtt ttggaagaag
     8281 gagacaaggt tcttattcca gcgcctgctt attcaggata tcagccatta gtggaactag
     8341 ctggtgctga attgatcccg atagatactt ctgacacagg gtttgtctgt cagccggaac
     8401 aatttgaacg agcatttgaa caatatggat cagctgtcaa agcagtcatt ttaaactatc
     8461 ctaacaatcc tactgggacg acattgtctg caaaacaatt gaaagcaatc gctgaggttt
     8521 taaaaaaata tccggtattt gttataagtg atgaagtata tgctgaattg acttatagtg
     8581 gaacgcatat gtccatagct acttatcttc cagaacaaac aatcgtgata agcgggttat
     8641 ccaagtcaca tgcgatgact ggttggcgag tgggctttat ttttgctcaa aaagccctta
     8701 ttgacgaact aattaaagtc catcaatatc tggtgactgc cgctacaacg atgtcgcaaa
     8761 aagctgcggc tgaagcgcta ataaatgggg tgaaagatag cgagaatatg aaagagcaat
     8821 atcaaacacg aagagactat ctggtccagc aattggcgcc tttaggattt gaagtgaccc
     8881 agccaaacgg tgcattttac ttgttttgca aactcccagc tgccattcaa actgactctt
     8941 ggcagttttg tgtagaatta gcggaacagg caaaagttgc gtgtatccca ggaattgcat
     9001 ttggcccaga aggagaaggt tatatccgga tttcttatgc tagcagcatg gaaaaattac
     9061 atgaggcctg caaacggatc aaagactttt taacagaaca gtgattcata gattttatca
     9121 aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag acgattgttt tttaaattta
     9181 gttcaaatga acgcgtttct tttgattcat aactaatgta atgttaagtt taaattgttt
     9241 agaatgattt taaaagaata ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca
     9301 ttgtaggtgc atctcatgga gggcatcaat cgatcttgga actattgaca cgatacggtg
     9361 aaaatgtaga tatcacgcta tttgaagcag gagactatat ttccttcatg tcatgtggta
     9421 tggagctgta tttagaagac aaggtaacag atgtaaatga tgtacgtaat ttcagaccag
     9481 aaaactttcc gcaatccaat gttcatattt taaataatca cgaagtaaaa acgatcgatg
     9541 ccgataaaaa aacagtaacg gttactagcg catcagataa cgaaacaaaa gaatatcctt
     9601 atgacaaatt gattttgagt tctggtgtca aaccaaacaa tctgccagta ccaggagcag
     9661 atctagaaaa tgtctacttg atgcgtggat acaattgggc aaccaaaatc aaagaaaaat
     9721 taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag
     9781 cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt gcttgatgtg atcgatagac
     9841 ctttaggcac ttatttagat gctgagatga cagatatttt agaacaacac ctaaatgaaa
     9901 acggtattga agttttgaca aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg
     9961 aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct agtcattcaa gctgccggtg
    10021 tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc
    10081 aaacaaatga atacttgcaa acgaatcttc cagatgtgta tgcagtagga gatgctacac
    10141 ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta
    10201 gagaagcacg ttatgtagta aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg
    10261 gagtcgtagg ttcctctgca ttaagtgtct ttgactatca ttttgcagca agcggattga
    10321 atagctttac agcagcaaaa gcaggagtag atgtacgagt gtcttattat gaagatacgt
    10381 tacgaccaaa atatgtacca gaagaacaag gaaatccaaa agtgtccatc caattattct
    10441 cggatccaat gacccatcaa ttgctaggag gagcagtatt atctacctat gatgttacag
    10501 gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact gactttagaa gaactagcag
    10561 aagctgactt cttcttccag ccaggctttg accgacaatg gagcatgttg aatctagccg
    10621 ctcaacaagc gttaggagaa gaaccatttg tagaataaga aaacgaaata tttgaaaaga
    10681 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagtaaaa acagcttctt
    10741 atgtttttaa cttggctgga cttattaccg cagatgcaag cttttgaaca ccgtttattt
    10801 tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc
    10861 taacttttgg aggtcggtat tttttatggt taaatatact tttgaactta aattaaagat
    10921 cgttcatgat tatttggatg gcaaaggcga ttggaccgta aaaatccaga tcaagagatt
    10981 gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt atggctgttt acggatgacc
    11041 aatgaactaa gaaaccgcgg tttctctatc aataaaaaga aagttcagcg cttgatcaag
    11101 aagctaggaa taaaagtaac ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga
    11161 ggaaaagt
//
LOCUS       DADJYG010000083        11159 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.085, whole genome shotgun sequence.
ACCESSION   DADJYG010000083 DADJYG010000000
VERSION     DADJYG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.085"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            6..305
                     /locus_tag="JZ382_001756"
     CDS             6..305
                     /locus_tag="JZ382_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2427940.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="JZ382_001757"
     CDS             378..1739
                     /locus_tag="JZ382_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY2427941.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="JZ382_001758"
     CDS             1761..2021
                     /locus_tag="JZ382_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427942.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="JZ382_001759"
     CDS             2035..2787
                     /locus_tag="JZ382_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY2427943.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="JZ382_001760"
     CDS             complement(2881..3486)
                     /locus_tag="JZ382_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2427944.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="JZ382_001761"
     CDS             complement(3539..4513)
                     /locus_tag="JZ382_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAY2427945.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="JZ382_001762"
     CDS             complement(4891..5670)
                     /locus_tag="JZ382_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2427946.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="JZ382_001763"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="JZ382_001763"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAY2427947.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="JZ382_001764"
     CDS             7161..8558
                     /locus_tag="JZ382_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2427948.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="JZ382_001765"
     CDS             8639..9079
                     /locus_tag="JZ382_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAY2427949.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="JZ382_001766"
     CDS             9227..9553
                     /locus_tag="JZ382_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY2427950.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="JZ382_001767"
     CDS             9554..10303
                     /locus_tag="JZ382_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427951.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="JZ382_001768"
     CDS             complement(10383..10838)
                     /locus_tag="JZ382_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAY2427952.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3543 a         1954 c         2292 g         3370 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagttt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactat
//
LOCUS       DADJYG010000084        11115 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.086, whole genome shotgun sequence.
ACCESSION   DADJYG010000084 DADJYG010000000
VERSION     DADJYG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.086"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(189..1037)
                     /locus_tag="JZ382_001769"
     CDS             complement(189..1037)
                     /locus_tag="JZ382_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2427953.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1201..2103
                     /gene="gnd"
                     /locus_tag="JZ382_001770"
     CDS             1201..2103
                     /gene="gnd"
                     /locus_tag="JZ382_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAY2427954.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2105..3106
                     /locus_tag="JZ382_001771"
     CDS             2105..3106
                     /locus_tag="JZ382_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAY2427955.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3103..3387
                     /locus_tag="JZ382_001772"
     CDS             3103..3387
                     /locus_tag="JZ382_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY2427956.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3420..4217
                     /locus_tag="JZ382_001773"
     CDS             3420..4217
                     /locus_tag="JZ382_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY2427957.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4231..5064
                     /locus_tag="JZ382_001774"
     CDS             4231..5064
                     /locus_tag="JZ382_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2427958.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5082..5939
                     /locus_tag="JZ382_001775"
     CDS             5082..5939
                     /locus_tag="JZ382_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2427959.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5961..6470
                     /locus_tag="JZ382_001776"
     CDS             5961..6470
                     /locus_tag="JZ382_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2427960.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6507..6908
                     /locus_tag="JZ382_001777"
     CDS             6507..6908
                     /locus_tag="JZ382_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427961.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7020..7373
                     /locus_tag="JZ382_001778"
     CDS             7020..7373
                     /locus_tag="JZ382_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427962.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7407..7850
                     /locus_tag="JZ382_001779"
     CDS             7407..7850
                     /locus_tag="JZ382_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY2427963.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7867..8952
                     /gene="uxuA"
                     /locus_tag="JZ382_001780"
     CDS             7867..8952
                     /gene="uxuA"
                     /locus_tag="JZ382_001780"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY2427964.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8945..9658
                     /locus_tag="JZ382_001781"
     CDS             8945..9658
                     /locus_tag="JZ382_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAY2427965.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10410..10610
                     /locus_tag="JZ382_001782"
     CDS             10410..10610
                     /locus_tag="JZ382_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY2427966.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3726 a         1741 c         2403 g         3245 t
ORIGIN      
        1 tgttatcgca tctaacaagt aactcgaata ataccaatac aaccttctcc aaagatgcga
       61 tgacatacgt tctaacccac cgactattga tgttctgtac cacccttgca gaaccaaaaa
      121 agccactacc aaatatttgg tagtggcttt ttttgtttta tggatttttc gctttttctg
      181 ttatggtgct attctttgat ttcagaaata gtatgtcgaa ctttagcgat agaacgacgt
      241 gattcttttt cgttgtaaaa catcaaaatc acatatagag agtccaaaat gcttagttga
      301 gaaatacggg aagctaaagc ttcagatcgg aattctgttt cttcagaaat agaaataaaa
      361 acgacatcgc caagtttagc aagggaagaa ttagcttggc tagtaataac gattgttttt
      421 gctccagttt ttcttacggt ttcagcaata tggattgttt cttttgattg ccctgtgtga
      481 gaaatcaaaa cagcacagtc tttttccgtc aataaagaag cttccatcaa ttgaatatgg
      541 taatccgtgc tgtgagaagt tgcgataggg gaacgcaaaa atttatgata agcgtcagtt
      601 gctacaatct ctgaaccgcc aacaccgaaa aaatagacac gtttagattc attgataata
      661 gtagccgctt tttttagatc cttttgttgt aacaactttt ttgtgtcatt taaagttgaa
      721 atactagact cgaacacctt ttgcgccata gtgagctcag tatctgtttt ctgaatattt
      781 tcgtggatag aaatagcaga tagatcattt tcttggatca acatggccat tttaaaatct
      841 ttaaatccat tataaccaag tttttttgta aattgaaaaa aagtagagtc agcaatgccc
      901 agttcacttg ctaaattgct aataggacta tgacttactt tgcttggatt ttctaaaata
      961 taatcggcga tagcctgttc ttttgggctg aagtcgttgt acattgtttt gatttgtagt
     1021 cttatggaga cgctcatgag aattcctcat tttcataata agattatcat tttttccgtt
     1081 ctcattaatg ttaaagatac aaggagagta ggaaaaaatc aaacaaaaaa tatttttgta
     1141 aaatctattt acaaaaaaat ttttttgtat aatgagggta taagaaatgg aggcgctatc
     1201 atggaaattg gtgtaattgg tttgggaaaa atgggattga atttagcttt aaacttaaaa
     1261 gagcaccatc acaaagtaca aggactcgac atttcaagta cagctgttag tcaggcaaga
     1321 gaaaatgaca ttcctgctta tcaagagatc tctgaatttc tagctacttt tgagcagaaa
     1381 aaaatcatct ggttaatgct tcctgctgga gagataaccg aaaacatgtt aagaaaactt
     1441 tttccaaaac tcggaccagg agacattatt attgaaggcg gaaactctta ttataaagat
     1501 tcaatccgtc gagcagaaga gtttcagaaa aatgaaatcg gttacttgga ttgtgggact
     1561 tctggaggaa tcgaaggagc acgacatcaa gcctgcttaa tgattggagg agataaagaa
     1621 acttttctat ccgtggaaca attattcaaa gatgtagcaa ttgaaagagg atatctgtat
     1681 gctggtgcat ccggaagcgg acacttcttg aaaatgatcc ataatggaat cgaatatgga
     1741 atgatgcaag caatcggtga aggattccaa cttgtacaaa aaagtgagta tgcttttgac
     1801 ttggaacaag tcgcacgtgt ttggaatcat ggttcggtga ttcgcggctg gctgatggaa
     1861 atcatggaag atcagttaaa cgaacatccc aatttaagta attatcgggg aatcgtttct
     1921 gcatcaggtg aagcaaaatg gacggttgaa acagcgctag aaatggctgt tccagttcca
     1981 gtaattgcaa ctagtttgtt catgaggaat ctttcacagg aagaagatag tttttcagca
     2041 aaagtagttt cagcactaag aaatgggttt ggcggacatg aaatagttga aaagggtgag
     2101 taaaatgcca gattatcata tagcaatcgt taattccagc agtttcggaa gagtctttcc
     2161 acaccacttg gaaagattaa agtcagttgg agacgttcat ttctttactg ttgatcaaga
     2221 gattccagga aaagaactag cggagttact taaaggattc aatatcatta ttgcaagtgt
     2281 tacgccattt tttcgacgag atttttttga aaacaaagat gaactgctat tgatttcccg
     2341 acatggtatt ggttacaaca atattgattt aaaagcagca aaagaacacc agacaatcgt
     2401 atctatcata cctgctttag ttgaacgaaa tgctgtagca gaaaataata taactaattt
     2461 gttgtccatg cttcgacgca cgacacaagc acaagcaaga gtcaaggacg atcgatggga
     2521 agatcgggcg gagttcgtcg gtaggacgct gtttaacaaa acagttggtg tgattggtgc
     2581 aggaaatacc ggaagcggag taatcgaaat tttgcgtaac ggttttcgct gtgatgttct
     2641 agcttatgat ccttacaaat cagatttata catcgaaagc tttggtgcaa aaaaagtaga
     2701 acttcaagaa ttactggaac aatcagatat catttgttta tgtgcgaatt tgacagaaga
     2761 gaactatcac atgatttctg ataaagaagt cgatcagatg aaagaaaatg tttatctatc
     2821 taatagtgca agaggcgctc tagttgatga agaagcgatg attaatggtc tgaaaagcgg
     2881 aaagatcgca ggatatgcca cagatgtttt agaagaagaa ccgggacgtc aagatcatcc
     2941 gtatttagcg tttgaacaag tagtgattac gccacatata tcggcctaca caagggaatg
     3001 tttagaagca atgggtgaaa aatgtgtcag agatgtcgaa cagatcagtc agcaccttct
     3061 tcctgaacgt tctgtccaga caaagagcaa ctatataaaa ggatgatgaa gaaaatggtg
     3121 aacggtttaa aagtgaaaac aggccctcaa ttttatttat atgaagaagg tggtatttca
     3181 aaagtttctg atctcttgaa atcatacggc gcgaaaagag tactggtcac ccacggaacg
     3241 gtatcatggg aaaaggcgct acctaaatta gtatttttga atgacgaaac gattcaattt
     3301 ttttatcatc gttattctgg agaatgtagt tatgctgaag ccagacgaat agctacaatc
     3361 atcaaaaaaa tgaaatcgat ttcgtagttg gcgttggcgg aggaaaactg accgatctag
     3421 tgggttatag tgccaatctt gcaagaatcg atttcggagt catccctact ttagctagta
     3481 attgtgctcc atggacacct cttgctgtca tgtataagga gaatggacaa gcagaaggga
     3541 aaacagaaca cttttacaga caagctgctt tttttataac agatccccaa ttagtgattg
     3601 attcccctgt tccatatttc attgccgggt tagcagatac actagcaaaa tggtatgaat
     3661 ctgagctcat cttagaacaa gattttttgc aagatgaatc atttttgaag ctagcacaag
     3721 atactgcgaa gatctgcaaa gaagagattt tgaatcattc tgctaaagca atccaagata
     3781 tggcacaaag aaaactgaca tcggagttca aacagctttc agagattatc tttgctgttg
     3841 ctggtttagt tggaggatta ggagataaat acgcaagaaa tgtggcagca catgctatgc
     3901 atgacgctat cagtaaatat attccggaaa gccatcgttt tctacatgga gaaaaagtag
     3961 cgtatgggat tttctatcaa ctggcactag aaggtcgctg ggaaaaaata gacgagttat
     4021 atcctttgta tcgggaatta gaactgccgg tttctctaga acaaatggga ttatttccga
     4081 aagataaaca gataatagat aaaatggctg aatttattga ttcgaaagag aaagtgcatt
     4141 tgattcctat agaggtaaca cagaaatcac tgatacagtc aatgatggaa ctggaaggtt
     4201 atatcagaaa gatctgaagg aggaggacac atggaaacat taagtgtagt acaaagtttg
     4261 ttgattgctt tatgggtagc cgcggttatg tcccgctggt taggtggagg agccacgttg
     4321 accttacgtt tttctccttt gatgactgga ttagtcgtcg ggttgatcat gggggaagtt
     4381 gcacaagcga tgattgttac agccgctttg caaatgattt atatgggggt tttttctcca
     4441 ggaggatcaa tgccagctga accatctatt gcagcagcta tcgcagtacc agtcgcatta
     4501 ttaggggatc tgtcgccaga agcagcgatt gccgttgcgg tgccagtcgg attgctcgga
     4561 agttacttgt atcagtttcg ttttttcatc aatacttttt taggtaaata tacagacaaa
     4621 gctgtagaag atttgaatac aaaagctatg gcccgatcaa ttattcttta tccaacggtt
     4681 gcctcatttt tattattcgt tccgcttgtt ttcattgctt tatatttagg ggcgccattg
     4741 atcgctgatg taatcaaagc tttagaggga acagtcgtta tccacattct tgaagtagta
     4801 ggtggagggt tagcggcaat cggtatcgcg acaactgtat acgttatcgg tcgaaaagat
     4861 tatctggtct ttttcttcct tgcttatttt atgagtgttg ttttgaaatc tttagatatt
     4921 acgatggtta cttatgccat ttttgggatt attatcgctt tgatctttgt ccagtcgcaa
     4981 aaagggaaac aagtacagcc tgccacaagc aataacaatt cagcatcttt tgatgacgaa
     5041 gatgactatg atgacggatt ttaatttagg aggaatggga aatggctgaa ataatggata
     5101 atcaaacagg tgctcctgaa gaaatcacga aaaaagatgt aacaaaagcc tatctgagat
     5161 ggcattttgc aaacgaaatt cctcactctt ttgaacgtta cttggcgcca tcactactat
     5221 acggaatgat gccaatcttg aaaaaacttt ataaagatga agactcttta aaagcagcat
     5281 ataaacggca gttactgttc ttcaacactc aattaagctg gggtggcggt gtgattactg
     5341 gcctaatgtc ttctatggaa cagcaaagag cagaagaaga gtatgaagaa aaagagatct
     5401 tgatgcagga tgacttgatg tataacacga aagcgggatt gatgggggca ctagcaggaa
     5461 tcggagatgc aatcgattca ggaaccgtcc aatatatttt tatcgcaatc gcggtacctt
     5521 gggcacaaca gggaagtcct ttaggcgcac tttttccttt tatctgcttt gggttgtatc
     5581 aggttttact aggggtattt ttcgcaagac aagctttcac tatgggccgt aatgctacag
     5641 gcctaaagca aaacactggt gttcagacag cgattgaaat gctatcagtt ttagggttgt
     5701 ttatgatggg ggtgcttgcg ggaaactatg taaaagtaga atcttcattg aaatttgcga
     5761 tatccggacg tgagtttgtt atccaggaaa ttcttgatca aatcgtgcca gggatattac
     5821 ctttagctgt cgtaatgggt gtgtacttgt tctatacgaa aaaaggattg aaagtcacac
     5881 aagcattatt atggctgact ggaatattaa ttgtgctagc aggaatcggt attttataaa
     5941 aagaagggaa cgtgaataag atgggtgtag tcaatttagc gcgtgtggat gagcgtttga
     6001 tccatgggca agtaatggta acgttatctc aaaaaagtgg agtgaattcc atttttgttg
     6061 tagacgaagt tgtagcaaaa gataaattta tgagagatct ttacaaaaat gcaggaagcc
     6121 gtacaggaca aaaaacaatt gtcattacac cagagaaagc taaattctat tgggatgaat
     6181 atcaatttaa agaatacaat tgtattttga tcgcaaaaac agtccaaacg atttacgact
     6241 tagtaaaaca cggaatcccc atggaagaat tgaatattgg agggattgct caaaaaaatc
     6301 ctgatacaga tcttttagtg acgaaatctg tttatttaaa taaatcagat gctgaaaaat
     6361 tgaaagaaat gaatgaacaa tatggcgtaa agaatattta ttttcaagcc actccatctg
     6421 cacctaaaac aggattaaaa gatgtattga acaagtttga tttgtcataa acaaatgctt
     6481 agaaaaaaga taaagagggc gaaaaaatga agaagaacga gctgtttcgt gattgggaat
     6541 ttcgttatcg ctatatttac cgtaagcgcc gtacaaaaaa gagtaaacag cgttttcttt
     6601 cagcattagt atcagatatt tacagtatga gaacagatgt gacagtcatt gcttacgata
     6661 cgctagccta ccgatcaaaa aatatttatg ttggagatat cgaaaaagca gagaaagtta
     6721 tctgtactta ttacgataca cccgttcatg cattaggaag ttatttcatg tttgattgga
     6781 aagaccaaag gaaaaaaaca atctactcta tattattgtc cttcattttg ttgttcagtt
     6841 taggttggtg gggaatgatg atatataaca aaaatcctca tcacgtattc gatctgctat
     6901 ccgtataaac aggtatcact gtactagcat ttgggagcta ctttttctta ttgggaaaag
     6961 cagctcgcgg atggcctagc agacaaactt ttattcgaaa tacttcatcc atattaacga
     7021 tgctagaaat gatccgtacg attgatgatc cgagtgtagc gtatgcgttt gttgatgaag
     7081 gctgttatgg agaaaagggc ttagacagcg tgagatcggg tatgaaaaaa gaggctattc
     7141 ttttttatct agactctgtt ggggcagata caccgctaca gttttcggga aattattttt
     7201 caaataaaga acagtggctg aaacaagttg ataagttgaa agaaaaaaac gtcaattata
     7261 tttttagtgc aagaaaaaag caagcgcagt tcttctattt gaccaaaaca gatttacgag
     7321 ggaaaacatt taattggcaa aatgccaatc agattattgc tctttttcga taagtaaaaa
     7381 gtgcaaaaca aggagtgaga agactaatgt tggaaatcat gatcgttaca catggtaagt
     7441 tgagcgaagg actgaaagat gcggcggaag taattgtcgg gacatcgagc catattgaaa
     7501 caatgtgctt agagtctggg gaggacatcc aaacattcgg agagaaggtc aaagaaaaat
     7561 taactatgat caacacagca gaacccatgc tagtatttgt tgatctaatt agcgcaagtc
     7621 cgtacaatca agcgctcttg gcagtcaatg aactgactga agaaaagaaa gcagccactt
     7681 atgtcatagg gggcgtgaat ctgccaatgt tgttagaagc aatcaatcat cagatgcttc
     7741 acacaccgat agaagaagcg gtggaacaaa ttgtcaaaca aggaaaagat aatttaactg
     7801 tttggcatgc ttcagcggaa gaaaaaaaat tagatgagga cgatttttag aaggaaggct
     7861 gaaaagatga agtggggatt tagatggtat ggtgaaaaag gggattcgat cccattaaaa
     7921 tatgttcgcc aaataccggg aataaatggg ataattggaa ctttgctgac taaaatgcct
     7981 ggagatgttt gggagatttc agaaattgag gagttgaaag catctgttga gagccaagga
     8041 ctggaactgc ttggaattga aagtgtagcg atccacgatg caatcaaggc agggacagat
     8101 gaacgagatt actatattga taactatata cgaacaatca aaaacttgtc tcgatgtggg
     8161 gttcgattga tttgttatag cttcaaacca atttttggat gggcgaaaac tgatttgttt
     8221 aatgaaaaca acgatggaag ctattctctt ttatacgatc agaaaatcgt cgatcagatg
     8281 gagccaggag aaatgtatca attgatacac agccagtcaa agggattcaa attgccgggt
     8341 tgggaagaag aacggttgaa aaaattcgct cagctggaaa aactgtatga aggaatgacc
     8401 gaagaagaat tatttgaaaa tctgacttat tttttagaaa aagtgattcc agtgtgcgaa
     8461 aaagcagatg taaaaatggc tatccatcca gatgatccgc catgggaact ttttggtttt
     8521 cctagagtga cgaaaaatct tggcgacttg aagaaaattc taaatgcagt agattctcca
     8581 tataatggac taacgctgtg cacaggatct ttaggggcaa atccggaaaa cgatttagtt
     8641 gaaatcatcc atgaggtagg ttcgcgcatc cattttgtcc acttcagaaa cgtggagttt
     8701 ttaggagaaa gaaagttcaa agaaacggca catccctcta cagaaggctc ccttgatatg
     8761 tatcatatta tgaaagcgtt ggtgcaagaa gggttcgacg gtcctatacg ccctgatcat
     8821 ggtagaaccg tgtggggtga agtggctatg cctggatatg gcctgtatga tcgtgcaatg
     8881 ggactcactt acatgcaagg tctatatgag gcgataagta aggaaaaacc actgggagag
     8941 cattatgaat aaaatcaagc tcttcatctt tgatatggat ggccttttat tagaaactgg
     9001 acgtttagct tatcgtgctt atgtaaaatc agcacaaaag tatgattatg aaatgcgtaa
     9061 ggaagtctat tatttactaa ctggccaaac agaaatggcg ataagagaac aaatgggcat
     9121 tttgtacgga gaagatgttc catatatcaa atggcgagaa gcaatcaatc aatataaaga
     9181 aaaaatcgtg aaagaagata agagagtata tacgaaaaaa ggagcagaag aaattttatc
     9241 ttttgcaaaa gaaagaggaa tccatacaat cgtggcttcg tctaatacac gggtaaaagt
     9301 agaaatgtat ttaaaaatgg aaaatctata tgccttgttt gatcaaatca tttcagggga
     9361 tgatgtaaaa aaaggaaagc cggaaccaga gatctttttg aaagcttgtt caaaaatgaa
     9421 tattcctcca agtgaagcac ttgtatttga agactcgatt gcagggatcg aagcagcaag
     9481 gcgagcgggc attttgtctt ttctcatcaa agatcatttg atagggttgc ccgatcataa
     9541 tgggaagcat aagttgaaag tagatgtaag tttatatgaa tccaataaag cagactatga
     9601 attttatgac ctgcatcaag caaaacgatt tttagaaagt aaagggttgt atctctaata
     9661 aagaggacta ttttctagtc ctctttattt attgtcctgc acgactgagg gaacagatga
     9721 aaaagaagta attttttatt ttgaagtatc taccttattt gtagtgaaca atttagaaca
     9781 aacacaaaac gcatctactg ttatctacca gtagatgcgt ttaaagagaa gaacgagaat
     9841 cgtccatatg agggtttcca ctccttatgc agattttttt aattcttgca ctgcttgttc
     9901 gatcgtaaca cctgtcgcta catgattggt attttctttg tcaaaaacga caaatagatt
     9961 caaatctgta tccaaggtta ctcgatattg tatatctttg ttatagaatg tcatagccat
    10021 tctcctttct ttaaatactt gctgaaaggc aacgaacgcg ttgataacga aattccattt
    10081 ataagtatac caccaattga aaggttttca tagttaagaa attttaaagt caaaagttgc
    10141 agataaaagc aaataaacaa gaaaacaaaa agtgaatcaa tcgcggttat tcaacttttt
    10201 cttcgattcg aagacaatag aagacaatga attttatatt tcattgcatt atccttgtgt
    10261 tattagaaaa agcatgatat attaaatgag taatctattt tgaaacgtaa tttgagcgat
    10321 atatctacat agataaaact ccttttgcct atcaaatatt tattgcaacg aaaaaacgta
    10381 cattgatacg tatcaggagg aatttattta tgaataacgg tacagtaaaa tggttcaacg
    10441 cagacaaagg ttttggcttt atcactggtg aagatggaaa tgatgtattt gcacattttt
    10501 cagctatcca aggtgacggc ttcaaaactt tagaagaagg ccaagcagtg tcttacgatg
    10561 ttgaagatgg tcaacgtggc cctcaagcaa caaacattgt taaagcttaa taaaaattga
    10621 cttttaaccg tcttttcgaa agaaaagacg gtttttttgt acgaaaaaat tcattggtcg
    10681 ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc ttttatggta
    10741 gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat atgaatcgag
    10801 atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta cagtttcgtt
    10861 cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa gagggcggca
    10921 catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag gctatcctac
    10981 tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt cattaaatcc
    11041 cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt ttttttaata
    11101 acgtaataat ttttt
//
LOCUS       DADJYG010000085        11020 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.087, whole genome shotgun sequence.
ACCESSION   DADJYG010000085 DADJYG010000000
VERSION     DADJYG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.087"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            9..80
                     /locus_tag="JZ382_001783"
     tRNA            9..80
                     /locus_tag="JZ382_001783"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Glu,seq:ttc)
     gene            344..1537
                     /locus_tag="JZ382_001784"
     CDS             344..1537
                     /locus_tag="JZ382_001784"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY2427967.1"
                     /translation="MVERHLFTSESVSEGHSDKIADQISDAILDAILEQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1668..3140
                     /locus_tag="JZ382_001785"
     CDS             1668..3140
                     /locus_tag="JZ382_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2427968.1"
                     /translation="MKKETNVTLVTVSVFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIIFIWLYLNEPKIIHESKPMDIFGSFSLMAMLVTLLLGFQLLG
                     DEGMSPLVSALLLGSIVLLGIFIFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMETHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKRGKIKQ"
     gene            3223..4011
                     /locus_tag="JZ382_001786"
     CDS             3223..4011
                     /locus_tag="JZ382_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY2427969.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQATTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVADYGLAVIMPSTDLGWYTDTAYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKAAFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKTVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWVFWDQWIQRALVWLMEEK"
     gene            complement(4078..4746)
                     /locus_tag="JZ382_001787"
     CDS             complement(4078..4746)
                     /locus_tag="JZ382_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAY2427970.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENNR"
     gene            4861..6573
                     /locus_tag="JZ382_001788"
     CDS             4861..6573
                     /locus_tag="JZ382_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427971.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKTFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPVEERSSNFSGGQKQRLSITRGVIGDPKILILDDSTSALDARSEKLVREA
                     LNRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6576..8345
                     /locus_tag="JZ382_001789"
     CDS             6576..8345
                     /locus_tag="JZ382_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427972.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAILIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEMFDEKDE
                     VKPENGKAFDGVEQGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIREYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEIINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8551..9117)
                     /locus_tag="JZ382_001790"
     CDS             complement(8551..9117)
                     /locus_tag="JZ382_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY2427973.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATREKLEQHQLEERVSLFLQGHETLGEVIAEQQDLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9117..10073)
                     /locus_tag="JZ382_001791"
     CDS             complement(9117..10073)
                     /locus_tag="JZ382_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY2427974.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKQNVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10073..10729)
                     /locus_tag="JZ382_001792"
     CDS             complement(10073..10729)
                     /locus_tag="JZ382_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY2427975.1"
                     /translation="MKYKTSFQVTGSFFLVLFFILGWIVKFHLSWLSGFDQWFTDIVR
                     ASYPNLNSYQLFMTKFGNPLTVIILFACTALWMWYKKKRAETVWFVSGFIVIAGIINP
                     LIKLFFMRERPSLTHLVVETSYSFPSGHAAGSMILYGSFLFLLPAFVQKRTLRTLLQL
                     LMVFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3394 a         1939 c         2330 g         3357 t
ORIGIN      
        1 attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg gtaacacggg
       61 ttcgaatccc gtacgggtca tggaagatac ttgatgagta tctttttctt tttatctgaa
      121 agaatgattt taaaaggctt agcagagtta tctttataga tgattgtgct aagctttttt
      181 ggataaatct aatgtaaaat atttatattt tttaagcaaa aaaatgaaaa aatacgagca
      241 gaaaactgtt ttatctgcta tacttggttc gttgcaagtt cccgaaagga tccgcaggac
      301 ggagatgcct tgtaaccgaa tttaactagg gggaatcatt ataatggtag aaagacactt
      361 atttacatca gaatctgttt ctgaaggaca ttcagataaa atcgctgacc aaatcagtga
      421 tgcgatcttg gatgcaattt tagaacaaga cccaacagca cgagtagcgt gtgaaacatc
      481 tgtaacaact gggcttgttt tagtatttgg agaaatatct acgacggcat atgtagatat
      541 tcaaaaagtc gtacgggaaa caataaaaga aattggttat acacgtgcaa aattcggatt
      601 tgatggagat acagcagctg tattggttgc gattgatgaa caatctcctg atattgctca
      661 aggagttgac gaagcacttg agatccggga tgaagataaa aaagacgtat tagatgaaat
      721 tggtgctggc gaccaagggt taatgtttgg ttttgctgta gatgaaacac cggaattaat
      781 gcctttacca atcgctttga gtcatcgtct ggtacgccgc ttggcagatt tgcgtaaatc
      841 aaatgaatta acgtatttac gtccagatgc aaaatctcaa gtcacagttg aatatgatga
      901 tcaaggacaa cctgaacgtg tagatacaat cgttatttca acacagcatg atgatgcagt
      961 agataatgaa acaattcgtc atgatgtcat cgaaaaagta gtgaaggaag ttattccagc
     1021 tgaattatta gatgatcaaa caaaatatta tatcaatccg actggccgat ttgtcattgg
     1081 tggtcctcaa ggggatgcag gattaacagg aagaaaaatc attgttgata cgtatggcgg
     1141 ttatgctcgt catggtggcg gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc
     1201 tgccagctat gctgcacgtt atattgctaa aaacatcgtt gcagcagggc ttgctcgcaa
     1261 agcagaagta cagctagctt atgcgattgg tgttgctcaa cctgtctcga tctcaatcaa
     1321 tactttcggt actggaacgg ttccggaaga agaattgatt gctgcagtga gagaaaactt
     1381 cgatcttcgg ccagcaggaa tcatcgagat gcttgatttg cgtcgtccga tttacaaaca
     1441 aacagctgct tacggtcatt ttggccgtac agatgtggat ttgccttggg aacatacgga
     1501 caaagtagat gcattgaaag caagtttagc aaaataagct atattcagct ggaacgatcg
     1561 ccgaatggtg gttgttccag ctttttttat ggcagtgcac ctcttgaaaa agatagtgag
     1621 tatttatcta tttttagatc ctgtcatgat gaaaaaggag gcataagatg aaaaaagaaa
     1681 caaacgttac tttagtcaca gtgagtgttt tcattgcaac gttcatgacg gctattgaag
     1741 ggacaatcgt aacaaccgct atgccaacga ttgtcggctc attacatgga atggaaatca
     1801 tgaactgggt attttcgatt tatttgctga caaacgctat gcttactccg atttacggaa
     1861 aacttgcgga taaagtggga cgaaaaccaa tttttatttt tgggacatta ttgtttatta
     1921 tcggttcgtt attttgcggt ttatctcaaa gcatgattgg gttgattgct gctcgtgcga
     1981 ttcaagggat gggtgctgga gcaatgatgc cagtagcttt gacgatcatc ggcgatcttt
     2041 attctattga aaagcgagca aaagttttag gtttgaatag ttcagcttgg gggattgcca
     2101 gtgtttttgg accgcttgcg ggaggattca tcgttgaaac gatcagctgg cactggatct
     2161 tttttatcaa tgtgccgatc ggaattctat taattatttt tatttggcta tatttaaatg
     2221 aaccaaaaat catacatgag tctaaaccaa tggatatttt tggtagtttt agtttgatgg
     2281 ccatgcttgt cacattgctg ttagggttcc aattattagg agacgaagga atgagtccgc
     2341 ttgtttctgc tttgttgcta ggaagtatcg tactgttagg tatttttatt tttgccgaaa
     2401 aaagagctaa agatcctgtt atcatgatgc atttattcaa aaatccacta tttgttgtgg
     2461 taaacttggc tgcagcattg gctagtggtt ttttgatggg aatcgatgtt tatattccta
     2521 tgtggatgca aggtgtatta ggactgaatg cagcaattgg tgggttggta ttagcaccat
     2581 tatcactagt ctggatggtc ggttctttcc ttgcaagcaa gtggatgact caaatggaaa
     2641 ctcattgggt cttgagaatt ggattactaa tcgcagctat tgggggcgct tggttgtatc
     2701 ttatgccttt cagaagtaac tatttatggt tttttgctat atcaacggtc atcggtatag
     2761 gcttaggtgt gaccatgact acctcgactg tcagtgcaca aaatagtgta tctcgtgatc
     2821 agcttggtgt agcaacttct tttaatacgt tagttcgaac aattggtcaa acagttatgg
     2881 tttctatttt tggtttattg ataaatgggg taactaaacg agaacttgca gcagcaaatt
     2941 taacaaatga tgcagatatc atgaacaaac tggtgaatcc acaaacggct aaatatatcg
     3001 atgcggatat tctgttgcca ttaagaaaaa ttctttatga tgggttacat tccgtttatc
     3061 tagttggctt gttactcgtg atcattgctt tggtcttaac gcttttcgtt catgaaaaac
     3121 gtggtaaaat aaaacagtag tttcggtcga gacatgtact ctgccgaatc attcaaattt
     3181 aaagttattc aaaaataact tcttactaag gaaggcgaaa atatggcatt tattcaagca
     3241 aatatttatt cgaatgtatt agaaatggaa gtcaatctaa atgtgatact cccacaaaca
     3301 actaaaaagg tgattggcac cagttcagag caagcgacaa cggacatacc tgttttatat
     3361 ttacttcatg ggatgggtgg caatcatagt gtatgggaaa gacgtacgtc gattgaacgt
     3421 tacgtagcag actacggatt agctgtcata atgccatcta ctgatcttgg atggtacaca
     3481 gatacggctt atgatatgaa atattggacg ttcattgcag aagagctacc tgagatttgt
     3541 catgaactat tcccacaatt aacaagaaaa agggaaaaaa catatgccgc tggattatcg
     3601 atgggcggat atggcgcatt aaaactaggc cttgcaaaat cagaaaattt cgcagcagta
     3661 atctctcttt caggcgctgt ttctgtagga aaccgaatgg atgatctttt gatggttcgt
     3721 aaagcagcct tttgggaagg aatctttgga ccattggatc aaatcgaagg ttcaaagaat
     3781 gatccacaat atttattgaa agaattagta aaaagcggaa aaacagtccc aaggttttat
     3841 atggcttgtg gggaatctga ttttctttat caagcaaatc aggaaatggt atctgctatg
     3901 caggaacaag gcttagcggt cacctttgaa caaggacctg gcgatcataa ttgggtcttc
     3961 tgggatcaat ggatccaacg tgcattggtg tggctgatgg aagaaaagta aaaaagagac
     4021 ttaaaaagat agctcctata gagatcactt tgttggtgac ctttatagga gcttttttta
     4081 tcgattgttt tctcttcttt tattttgttg gcataaacct tcaatatagg tttgtgcact
     4141 cgttagaaag agtgcttttt cattcaattc tgcatgggac tgcatacttt ctttaaaacc
     4201 ctctaattga ttttcgacta cataattacg cataaataga actgtttctt tcaaaaattc
     4261 atttccgtcc ataatctttt gccgaagctg ttgttcatgc tgctgatcca ttagctgtcc
     4321 aatcaataaa ttgtggagag aggcaatggc ctgatgactt tgagtgactg aaaatccttc
     4381 acgaacaagt agttggatca tttgttccaa atgttctaat cgactcggaa aagcgggcac
     4441 agtattgatt tctaattctg ccccacaagg aaaacgtgtg taaaggtcat agtaattttc
     4501 catatactgt agcaactgtt cattccaggg caaagttgga tctggaagcg ctaattcttc
     4561 ttccattgtt tcagccattt tctgcaagag ctgttgttta tttttgaaat accaatataa
     4621 agcaggcgct tgaacgtgaa ttctgtttgc gatgtttcgc atggaaagct gcgaaagagc
     4681 ttgtgtttca gccaggatat caaaagcggc ctgaatgatg atggcttgag aaagctttat
     4741 ttccatttta tcaacctcgt gtattgcatt atctttttaa acatggtaaa ttgttagtcg
     4801 gtctaatttt acagtgttaa attagtcctg tcaatttaaa caaaggggtg taatcaacga
     4861 atgaaattaa tcttacgaca tgcgaaaaaa tataaagtag ctgtttttat ttccttgctg
     4921 tccgtagcag tcatggttgc agcagcatta tggcaaccta aattactaca gcaagtattg
     4981 gaagctatta ttacagaaga taatgatgaa atgaaaacaa tcggtattta tctgatcagt
     5041 ctggctttgc taggattagc tgcaggtgta acgaacacga ttttctctgc gaaagtagct
     5101 cagggcgtaa gtgcagatat ccgagaagaa gcttttcgaa agatccagac attttcattt
     5161 gggaatatcg aacagttctc tgctgggaat cttgttgttc gtctaacaaa tgatatcacg
     5221 caaatccaaa atttagtgat gatttctttg caatcacttt ttcgaattcc attcttattt
     5281 attggtgcct tcattttagc tatgatcacg atgccacaat tgtggtggat catcgtagcg
     5341 cttatcgtta cggtatttct tatcacggca ctttcattta cacggatggg taagcacttc
     5401 atgatcattc aaaaactgat tgatcgtgta aatagcattg caaaagaaaa cttactaggg
     5461 attcgtgtag tgaaatcttt tgtacaggaa gaaaaccaat tgaagacatt cagcaaagta
     5521 agtgaagact taacaaaaca caacatcata gtcggtacat tattttctgt gatgatcccg
     5581 tccttcatgt tggctgccaa tttggctgtg gttggagcaa tcttctttgt gagcgacctt
     5641 gttaaagatg atcctacatt aattggtgga attgcttcat tcatgaatta cttgatgcag
     5701 atcatgatgg caatcattat cggtggaatg atgatgatga tgacttcaag agcagccgta
     5761 tcgctgaagc ggattgcgga aattttagat gctgaaccag atcttactta tttagacgtt
     5821 ccagaacaag agttgacggg ttctgtcact tttgaccatg tttcttttcg ttatcctggt
     5881 gaggatacag atacgcttaa agatatcagc ttttctatca aacctggaga aatggtcggg
     5941 attgtcgggg cgactggtgc cgggaaatcc acactagcac agctgattcc tcgtctattt
     6001 gatccaacag aaggaaaagt agaagtaggt ggtgtagacc ttcgccaagt aaatgaaaag
     6061 agtttaagac aaacggtgtc ctttgttttg caaaaagcaa ttttattctc aggaacgatt
     6121 gctcaaaact tgcgccaagg aaaaaagaat gcaacagaaa aagagatgga acatgcttct
     6181 tctattgctc aagcaaaaga attcatcgaa aagctatctg atcgttacga agcaccagta
     6241 gaagaacgaa gcagcaattt ttctggtgga caaaagcagc gactatccat tactcgtggt
     6301 gttatcggtg atccaaagat cttgatttta gatgatagta cgagtgcatt ggatgctcgc
     6361 tctgaaaaac tggtccgaga agcacttaat cgagatttga agggaacaac gacgatcgtc
     6421 atcgcacaaa aaattgcttc tgttgtcaaa gcagatcgca tcttagtatt ggatgaaggt
     6481 agattagtcg gtgaaggaac ccatgaggaa ttggtagctg gaaaccgtgt ctatcaggaa
     6541 atctttgaaa ctcaaaaagg aacggaggag taagtatgac tgatttaata aaagcaagta
     6601 aatttttcta tcactatttg aagcggtaca aactctcctt tttctttatc tttatcacag
     6661 taattattgc tacctatcta caagtaaaag cacctcagta tatcggggaa gcttttcaag
     6721 aattagcaaa ttacgtaggt ggcttgatgc aaggcgtaga cgataaaagc aaatttgttt
     6781 ctgtaatatg gaaactcttg ttgttttatg tattagctag tgcagccaac tttatctata
     6841 gtattttatt cactcaagta gtagggaagt caacgaaccg gatgcgtatc ggtttattca
     6901 acaaattgga aaaattgacg attcgcttct ttgattctca tcaagacggt gaaattttaa
     6961 gtcgttttac cagtgactta gataatattc aaaatagttt gaaccaagca ttgcttcaag
     7021 tcatcacgaa tgctgtttta cttgtcggaa tcttgattat gatgtttcgt caaaatgtag
     7081 aattagcttg ggctacgatt gcttctactc cagtagctat tttgatagct gtcttgatta
     7141 ttcgaaaagc gcgaaaatat gtggatcttc agcaagatga agtgggaaaa ctgaatggct
     7201 atatggatga aaaaatcagc ggacaacgtg tcatcatcac aaatggctta caagaagaaa
     7261 cgattgaagg attcttagaa cataatcaaa gtgttcgcga agcaacgttc aaaggacaag
     7321 cttattctgg cttactattt ccgatgatgc aaggaatgtc actagtcaat acagcaatcg
     7381 ttatcttttt cggcggctgg ttagcattga atggcgattt ggaacgttcg gtggcgttag
     7441 gcttagttgt gacattcgtc caatattcac aacaatacta ccaaccattg atgcagattt
     7501 cttctgggta cagcatgatc caattagcaa tcacaggggc aagacgatta aatgaaatgt
     7561 ttgatgaaaa agatgaagtc aaaccagaaa acggtaaagc atttgatgga gtggaacaag
     7621 gacttgcctt gcaacacgta gattttggtt attctccaga aaaactagtc ttgaaggatg
     7681 tctcaatcaa tgtggacaaa ggagaaatgg ttgctcttgt tggaccaacc ggatctggga
     7741 aaacgacgat catgaatctg ttgaatcgtt tttacgatgt caatgatggg tcagtagtaa
     7801 tcgatggaat agatatccga gagtatgatc tagatgcatt acgtagacat gtaggaatcg
     7861 ttttgcaaga atccgtcttg ttctctggca ccatcaaaga caatatcaca ttcggtaaac
     7921 cagatgcttc agatgaagaa atcatcaatg cagcaaaaca agcgaacatt cacgacttta
     7981 tcatgacgtt agaaaaaggg tatgatacag agatatccga agaaaacaat ttgttcagta
     8041 caggtcaaaa gcagttgatc agtattgctc gaacgatcat cacaaatcca tctttattga
     8101 ttttggatga agcaacaagt aatgtagaca cggtaacaga agcaagaatc caaaaagcaa
     8161 tggatgaagc catcaaagga agaaccagct ttgtgattgc tcaccggctg aaaacgatct
     8221 tgaatgcaga tagaatcgtt gtcttgcgtg atggggaagt cattgaagaa ggcagtcatc
     8281 acgaattatt agcagaaaat ggattttatg cggaacttta tcataatcaa tttgtatttg
     8341 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
     8401 agcttctcat gtttttgatt tggttggatt tattaccgca gatgcaagct tttgagcacc
     8461 gtgaataaag aggttgtgaa attaacgctc taacctgagt attagagaaa aaaaacgcaa
     8521 aacatatcat ttatgttttg cgttttttat ttatcttttt ttctcaatac aatataaaat
     8581 aggcggctgg ttcttttggt tgataaaccg ataattcagc acattatatg tttcctgtgg
     8641 cagtttggaa caaaaagaat caaccatatc cagttctttt tctccgcctt catgaccata
     8701 gtagacaaca aggatcaatc gtccgcgagg cacaagtcgt ttcaatattt cttccatagc
     8761 tgtgcgtgta gtctctggca tagtgatgat tgctttatca ctttttggga gataccctag
     8821 attgaagatc cctgctttga gatcttgttg ttcagctatc acttctccta acgtttcgtg
     8881 gccttgtaga aacaatgaga cacgttcttc taactgatgc tgttctagct tttcacgagt
     8941 agcatcgatt gcttgttgct ggatatcgaa actatagaca tgaccagttt ttccgatgtg
     9001 ttctgctaga aaaaccgtat cgtggccatt gcccatcgtt gcatctacga cgtgatctcc
     9061 tggttgcaag atttcttgca gtaatgtatg gctgaaatgc aaagcagttt gcaacatcag
     9121 acactcacct ttttccgtac atcatatttt ccttggaagc tatctctgcg aagaagttct
     9181 tcatcaattg cattcaagac ttcccatttt tttaagctcc acattggtcc aatcaaggaa
     9241 tcccacggcg catcccctgt caaacgatgg atgatgatct cttttggtat catttccagc
     9301 tggtcgcaaa tgacattgac atagtcagga cgactcatta attgaagtcg accttcatga
     9361 tagtctcgca acattctcgt tttgcgcatc agatgcatca aatggagctt gattccttgg
     9421 atatcggaat ctaaaatagt gcgtctgaca ttttgtttca tcatctctaa actttcgcct
     9481 ggtaatccgt tgatcaaatg cgtacaaaca cggatattat gtttgcgtaa tttagccacg
     9541 ccatctaaat aagtttgata atcatgtgca cggttgatca gctttgaagt ttcttcaaaa
     9601 gttgtttgca atccaagttc tacccataaa tacattcgct cgttcagttc agcaaggtaa
     9661 tcaactactt catctggcag acaatccggt cgtgtaccga ctgataatcc tacgacacct
     9721 ggtaaattga ctacttgctc aaatcgttct ttgattatct caactggcgc atgggtgttt
     9781 gtaaaatttt ggaaataaac gatgtattgt tggacatgag gccatttttg atgcatcatg
     9841 tcgatttctt tctggaattg gattggaagt ggatcttcag gtgcaacaat catatcaccc
     9901 gaaccagata cactgcaaaa agtacaaccg ccatgggcaa cagtcccatc tcgattcggg
     9961 cagtcgaacc ctccatcaat cggcaccttg aaaattttcc cgccaaactc atttcgtaaa
    10021 gcataattcc aagaatgata tcttttattt ccttctgaat aaggaaaagt cattaaaagt
    10081 ccttctttct gtaagagtta tctatttgtt ttcttttttc tctatacact ggataagtca
    10141 ggcacagcca agaaagactg agtaagtagc cgccgatgat atcagtagga aaatgaacac
    10201 ccagataaat gcggcttatc ccaacactta ctataataaa caccatcagc aattgaagca
    10261 atgtacgtaa ggttcttttt tgaacaaaag ctggcagcag aaataggaaa gagccgtaaa
    10321 gaatcatact gcctgctgca tgtccactag ggaaactgta acttgtttca accactaagt
    10381 gtgtcaatga agggcgctct cgcatgaaaa atagtttgat caacgggttg atgattccag
    10441 caattacaat aaatccagaa acgaaccaaa ctgtttctgc cctttttttc ttataccaca
    10501 tccataaagc cgtacaagca aataaaatga tgacggtaag aggattaccg aatttagtca
    10561 taaagagttg gtagctgttc agattaggat aagaagctct gacaatgtct gtaaaccact
    10621 gatcgaaacc agaaagccac gacagatgga actttacgat ccagcccaaa ataaaaaaca
    10681 ggacaagaaa gaagctgcca gtcacctgaa atgatgtttt atatttcata caattcctcc
    10741 tttgaagcaa tgacttatta tagcattttt ggtccagtga aaaaaacggt aaagagataa
    10801 aaccaatgta tgatccctct ttgaataacc aatattctta taaaaaaaat aactgtaatc
    10861 ggaaggacat tttttggtaa tacaaatgaa atgttaggaa aataaaatca gtgtttttcg
    10921 aaatccttgt aaataagaac atagatgtag gaagaacttg aaaatgaaca gaagttgttc
    10981 attacggtaa attacaatat gattacaaaa aacaacaaag
//
LOCUS       DADJYG010000086        10990 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.088, whole genome shotgun sequence.
ACCESSION   DADJYG010000086 DADJYG010000000
VERSION     DADJYG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.088"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(239..607)
                     /locus_tag="JZ382_001793"
     CDS             complement(239..607)
                     /locus_tag="JZ382_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAY2427976.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            757..1239
                     /locus_tag="JZ382_001794"
     CDS             757..1239
                     /locus_tag="JZ382_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAY2427977.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1276..2286
                     /locus_tag="JZ382_001795"
     CDS             1276..2286
                     /locus_tag="JZ382_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAY2427978.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2401..3114)
                     /locus_tag="JZ382_001796"
     CDS             complement(2401..3114)
                     /locus_tag="JZ382_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2427979.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(3304..3894)
                     /locus_tag="JZ382_001797"
     CDS             complement(3304..3894)
                     /locus_tag="JZ382_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAY2427980.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            4378..4605
                     /locus_tag="JZ382_001798"
     CDS             4378..4605
                     /locus_tag="JZ382_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY2427981.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4621..5787
                     /locus_tag="JZ382_001799"
     CDS             4621..5787
                     /locus_tag="JZ382_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY2427982.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6021..7064
                     /gene="hrcA"
                     /locus_tag="JZ382_001800"
     CDS             6021..7064
                     /gene="hrcA"
                     /locus_tag="JZ382_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAY2427983.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7158..7721
                     /gene="grpE"
                     /locus_tag="JZ382_001801"
     CDS             7158..7721
                     /gene="grpE"
                     /locus_tag="JZ382_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY2427984.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7772..9601
                     /gene="dnaK"
                     /locus_tag="JZ382_001802"
     CDS             7772..9601
                     /gene="dnaK"
                     /locus_tag="JZ382_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY2427985.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9752..10918
                     /gene="dnaJ"
                     /locus_tag="JZ382_001803"
     CDS             9752..10918
                     /gene="dnaJ"
                     /locus_tag="JZ382_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY2427986.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3748 a         1854 c         2309 g         3079 t
ORIGIN      
        1 tttccttatt caaaagtttt gcaggaaaat tgctgaaaaa ataattttac tatgtagaaa
       61 attcaaagcc attcaatcag tgattttttt cagcacaaga aagactcctt ataaaaatag
      121 cagatacttt gtcaattaag tgtttaacca gggatgataa aaaggatagg attgaacaaa
      181 acctgtataa ggtgatgaat agtttcctcg ccagtaaaag gtttattcgt ataaagcttt
      241 atcgccaaaa tttatcatga ccttttttat cttcaatatt aaatgaaatc aattgttcaa
      301 tcaattccca gttgattaca cttttccaag gaactttgat gacgttatcc gtatatacgt
      361 agcctgcttc ttggatattt ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag
      421 aaaaatgatt tttagcaaaa ctaaatgcaa taataaaggt accatggtca gtaaacatag
      481 gttggttcca ttttacagtt gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa
      541 ggtctttgat cttttgttga tgttcttttt ctttgatttg attgatgatc ggctgaaatt
      601 tttccattct aaaacgcctc cattaagtgg tttgatttat tttaccagaa aaacaatagt
      661 tatgtataaa aaacgaacaa aacacaagta atgttgtaac cgctatattt atatgataag
      721 atgtctcaaa taataaaata gaaaagagac ataaaaatga ttgacttggc aaagaaattc
      781 ttacacgaaa atatatattt tgagttgtat catcatcgag ccatttatac aaatgaagat
      841 gctttggtaa taaagaaaga acaaggattt acaggtacag agaccaaaag cttattctta
      901 aaagataaaa aagataataa ctacgtatat ctgactttta ctacgaaaca aacggatttc
      961 aaagcattag ggaagatcgt cggtaaaagg ctttctattg tttcatcaga acgcatggaa
     1021 gaacaaacgg gacaaaagca gggcgctgtt tctccgtttg gttatgaaaa acagattccg
     1081 attattattg atgaagagct cttatcaaat gaaaaacttg tttttgcacc aggtagacca
     1141 gatcaaacga tggtcatcca aacggttgac ttaaacaaaa tcatccagct acttgagatg
     1201 ccgatatttt tgatgccggc aaacgaaaaa aacaattaac caagtaaaat agtgcatact
     1261 tggaggtgaa gcaacatgat aagttatcca ttattagaaa gaaatgcaac gattggggtt
     1321 actgcaccat cctcaggcgt atcagaggaa caccattcgt tactcgaatc agctttaaag
     1381 agaatggaag aaaaatcttt ccaagtagtt actggaaaga cgctttggac gcaagataag
     1441 gaaaaatcag gtccagcaag aaaacgagca gatgaattca atcaaatgat gcaagatgaa
     1501 aatatccaaa tgatctttcc gacttgggga ggagaattat tgattgagat tctcgatttt
     1561 gtcgattttg atgctctaca accaaaatgg atagtaggtt attcagatat tagtacgttg
     1621 ctacttgctg tcacattaaa aacagggata gccactgcac atggaactaa ccttattgat
     1681 ttaagaggag agtacttgga tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga
     1741 gaaggagaat cgattctaca gcaatcttct gaaaagtatc aaaaagtgtg gccccatgag
     1801 cattcatcat cttctgtttt ccatctcact gaaccaacaa attggcaatc agtcagcgga
     1861 gatgcagtaa ggatgaaggg acgattatta ggtggttgta ttgatacgat caggcattta
     1921 atcggtactc cttatggaga tgtccaatct tttaaaagaa agtataatga tggaaagccg
     1981 atcttgtggt ttctagaaaa ctgtgagcta tcgtcagttg atttaagaag atcacttgtt
     2041 cagatgaaac tggcaggctg gtttgatggc agctcgggga tcatgttcgg tagaagtgcg
     2101 gcagaggatt tgttagatta taaaatgaag gatgtatacg aagatctgtc aaaggaatta
     2161 aaactaccga tcttgtatga tattgattgt ggtcatgtac cgccacaact gacattgatt
     2221 aatggtgcct atgcagaagt agcggtagaa gatagaaaag cgactgtttt gcaaacgttt
     2281 atttaaaaaa ttttttcctg ccttggagtc tatcaaagac caataaatct aaatagaaaa
     2341 gaaagtgaaa gaagctgaca aaatgctgtc agcttctttt tgtataacat attagaaagt
     2401 ctaattatct ataactgtca catcgattaa ctgtttcacg tcattgggat ttcctgtagg
     2461 aatagctact acagatgcat tatcaatgat ttcttgagtt gtttctctaa tgaacgctgg
     2521 ataagatgtt tgatctgcag gcatttgcca atgtgaagga actggataac tagtgattgt
     2581 cccgtcatgg ttacttgaat acactaaaac tccctggtaa ctgtatttcc cagtgagcat
     2641 aaccacatct ttcggataga ctgcactccc tgcatcataa ggggcgatag gtgttcctgc
     2701 aggataatga ataacatgta attcgaaatc acccgatgta tctctaaaag tattacctct
     2761 aatagctagc caaacacgtg cgtactcaat ttgtagatca gaatatcctg ctaacggatc
     2821 tattgactga gaagactgtg aggaggttgt gtctattgtt gaagaagaaa tatttgtctc
     2881 aactgtacta ctagatgtag aagaagtatt gggctctgag actttactgc ttgttgaaga
     2941 tgtattcgtt tcagtagaag ctgatttctg agtacttgat ttttcagctg aactggtttg
     3001 tctgattgaa cttttgtctg tggtcgcaga tggatcacta ttttctttac taggagacgt
     3061 acatcctaca aggaataaag agaccaacaa taaaataaaa cctaactttt tcatataaaa
     3121 tacctcatta tttttttaat accagtatag cataatcctg aatttttgaa tccgtaaaac
     3181 cttgtctatg aatataaata gagtgtatct tctttgataa atgtcagaaa aagtgtgaca
     3241 aataatcatc agtaaaatta cttgtcacac tattgataag gagtatttaa ttgaagagaa
     3301 tgattaggag aaaggatctc cgtacttttt atagtaatca tagaatccaa gtttgtatag
     3361 acgaatcgtt ttttctctta tttcttctgt ttcaaaatgg actgcttgaa gaatcaagtc
     3421 tgtgaattct ataggagctg ttccattcgc agtaatcaaa ttacgatctt tcacagcttg
     3481 ttgttctata aaatcttctt tattctgata attttccgat tctaaccaag gaaattgcgc
     3541 atttccggtg tgtttgtaac cttgcaataa tccgctctga gctaaataat caactgcccc
     3601 acaaatagca gctactggta cgtctgtttt taacaattta tcgattattt gatataaatg
     3661 atgatttttt atgtcccatg aattgccacc gatcataata aaaaggtcaa ctttttcagg
     3721 tatttgttcg attaagtaat cgatttttgt ttttaatcct ccaatggaag tgacctctga
     3781 ttggatagaa gcagttttgt tttgccattt ctcattttgg tttagtgtgc tagtaatata
     3841 agcaccttcc caatcggcat attcctctaa tagaaacgta atcgcagttt tcattttcaa
     3901 gctctccttt actatctata acaaggacaa cagttttaac ctacagacat tcattaaaat
     3961 tgggtagcga cagcgaaaac aacaattctt aagttttaga cagagtatac agatagtcaa
     4021 cttaattaaa tacgaactaa ataaaaatga caggtaatca tttttgttta ccagcattat
     4081 aacattttgc atacatggat gacaaaagaa ccaattccca cttgcttgag tacaatatta
     4141 aaagaaactg gaaggcaaaa agtaattata gaaacgaaaa ataaatcata tcgggcactc
     4201 agacatgaaa acaaccctag agatatataa ctaggtaacc agtttaatga aagaaaaaat
     4261 catccaagaa gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat
     4321 gcgtatatga gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg
     4381 aaaatagctt ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt
     4441 accgtttatc gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt
     4501 ttagagctag gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa
     4561 cgcgatttta agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca
     4621 atgatagaaa caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt
     4681 tgcgagcata tctgctatta ctgtgatttt aataaagttt ttttagaagg gcagccagta
     4741 gatgagtatg tggaaatgct gctgaatgaa atgaaaataa tgctagaaag tcatccgaca
     4801 gcgaatttag aaacacttta cgtagggggc gggacaccaa cttctttatc tgctaaacaa
     4861 cttgatcgac tgttatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt
     4921 actgtagaag cgaatccagg tgatttgacc cgtgaaaaac ttcaagtaat gaaaaactat
     4981 ggcgtcaatc gtttatccat gggagtgcag acatttgata atcgcttatt gaaaaaaatt
     5041 ggacgtaaac acaccgcaga agatgtgtat caaactatgc aatttttaga agcagaaaat
     5101 tttaccaatg taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat
     5161 ctggatactt tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg
     5221 atcctcgaaa acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct
     5281 gatcaggaaa cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga
     5341 cgtcaccaat atgaaataag taactttggg ctctccggac atgaatctca acataacctc
     5401 atgtactgga acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga
     5461 gatattcgtt accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat
     5521 gaacgcccgg tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg
     5581 ttcttaggac taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa
     5641 aaaacaattg aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt
     5701 gttaatgaag aagaccgtgt ttatttgaca ccaagaggga cctttttggg gaacaatgtg
     5761 tttgagcgct ttttgtttga aaaatagttt aaagaatatt tgttttttat ctgaatttaa
     5821 ataaaaaaag ttcaaaaata caataaagga ctattgaaac gttgatataa ctggatttac
     5881 atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct
     5941 tgacaggaga agggcttctt gctatattta taagtggatt agcacttaga atatatgagt
     6001 gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca
     6061 tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattgatg tctgagggag
     6121 tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc
     6181 tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg
     6241 tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt
     6301 ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat
     6361 ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac cgccgactga
     6421 ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata
     6481 tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag
     6541 aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc
     6601 ataaacttcg tacggagatt ccgatgattc ttcataaata tttccaaacg acagaaggga
     6661 tgtctcagtt atttgacacc gttcttggtc atgtttttga agataaggtt tatgtcagtg
     6721 gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt
     6781 tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata
     6841 ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc
     6901 aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta
     6961 gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta
     7021 aacttgctga ttactaccgt tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct
     7081 atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat
     7141 gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg
     7201 ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg
     7261 aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat
     7321 acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc
     7381 aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc
     7441 tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg
     7501 tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc
     7561 ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga
     7621 ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac aaattacatg
     7681 atcgcgtgtt aagaccaacc atggtcattg ttgcacaata attgtgcgga tataaaactt
     7741 acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg
     7801 tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc
     7861 agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg
     7921 ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca
     7981 tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat
     8041 ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt
     8101 cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa
     8161 agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc
     8221 tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct
     8281 tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt
     8341 gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta
     8401 catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt
     8461 acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac
     8521 acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac
     8581 attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc
     8641 agtacgtcaa gcgctaaaag atgcaggttt atcacaatca gaaatcgacg aagttatttt
     8701 agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa
     8761 agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg
     8821 tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt
     8881 aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc
     8941 aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca
     9001 tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt
     9061 gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga
     9121 taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat
     9181 cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg taaaagatgc
     9241 agaagcaaat gcagaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt
     9301 agatgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga
     9361 agaagaagtg aaaaaagcag aaacagctcg cgatgaattg aaagccgctg ttgaagcaaa
     9421 caacatcgaa gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac
     9481 tgttaaacta tacgaacaag cggcacaaca acaagctcaa gaaaacccag aagcagctca
     9541 aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata
     9601 atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa
     9661 aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg
     9721 aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt
     9781 cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc
     9841 gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt
     9901 atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatatg accaatacgg
     9961 gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt
    10021 cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg
    10081 aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac
    10141 ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg
    10201 ggaagaaatc tgtcatactt gtggtgggaa cggtgcaaaa ccaggcacac aaccaactac
    10261 ttgtcataaa tgtcatggtg caggtacgat taatgtagaa cgccaaacac cacttggacg
    10321 agtgatgagc cgtcaaactt gtgatgtttg tcatgggaca gggaaagaaa tcaaagaacc
    10381 ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt
    10441 accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga
    10501 aaacggcgga ccatttggtg atttgtatgt ggtcttccgt gtagaagaaa gtgatatctt
    10561 tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt
    10621 gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg
    10681 aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg
    10741 tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca
    10801 acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca
    10861 agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt
    10921 tttctagtaa aaacgctaag tcattaataa ttaaaaacca gacgaacaat catatgttcg
    10981 cctggttttt
//
LOCUS       DADJYG010000087        10758 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.089, whole genome shotgun sequence.
ACCESSION   DADJYG010000087 DADJYG010000000
VERSION     DADJYG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.089"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(81..1343)
                     /locus_tag="JZ382_001804"
     CDS             complement(81..1343)
                     /locus_tag="JZ382_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2427987.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1336..2235)
                     /locus_tag="JZ382_001805"
     CDS             complement(1336..2235)
                     /locus_tag="JZ382_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2427988.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2380..4158)
                     /gene="uvrC"
                     /locus_tag="JZ382_001806"
     CDS             complement(2380..4158)
                     /gene="uvrC"
                     /locus_tag="JZ382_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY2427989.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4340..4654)
                     /gene="trxA"
                     /locus_tag="JZ382_001807"
     CDS             complement(4340..4654)
                     /gene="trxA"
                     /locus_tag="JZ382_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAY2427990.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4741..7101)
                     /locus_tag="JZ382_001808"
     CDS             complement(4741..7101)
                     /locus_tag="JZ382_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAY2427991.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7216..7763)
                     /locus_tag="JZ382_001809"
                     /pseudo
     CDS             complement(7216..7763)
                     /locus_tag="JZ382_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7821..8258)
                     /gene="zapA"
                     /locus_tag="JZ382_001810"
     CDS             complement(7821..8258)
                     /gene="zapA"
                     /locus_tag="JZ382_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY2427992.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8460..9377
                     /locus_tag="JZ382_001811"
     CDS             8460..9377
                     /locus_tag="JZ382_001811"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAY2427993.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9633..10544)
                     /locus_tag="JZ382_001812"
     CDS             complement(9633..10544)
                     /locus_tag="JZ382_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY2427994.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT         3041 a         2301 c         1808 g         3608 t
ORIGIN      
        1 taaaaaaata gaaataggtc tatacaaaaa agaagctggg aacaataaat gtccagcttc
       61 cttctatatt tttctatatt ttatgctttt tttcgttcat ttctcataat tgaaatcgat
      121 tgtttcaatg aactccataa tccgccttcg ctatacacga gtacattcga tttgtacatg
      181 tttgcagaca agatcatcaa taagaatgtt acggctacaa gtaccaacaa ggaaatcaat
      241 gctccaccca taccgactgt ctcatttgct aagcgaaccg gcataatata tgaagataag
      301 aatggaatat acgaagtgat ccgaataacg atattgtttg gatctgaagc tcctagtatc
      361 aatccaagca tatatccgcc tagagataga taagtcactg gcaaaattgc ttttgctgta
      421 tcttctgctt tgttgaccaa agagccacat aaagctgcta aaacagaaaa aatcaaaatg
      481 ccaaagatca tgaatagaag agaataccac aagaatggtc caatcaagtt atccaatgag
      541 ataccgtcta atatattttt gaccatttcc atatctttga attgactgta tcctatacca
      601 aaaataatac cgtataatgc catttgcgtt aatgcaacaa gtaaaacacc tgttagtttt
      661 ccgtaaaaat gcttttgtgc agtcgtactg gacaggatca cttccatgat ccgcgtgcct
      721 ttttcagaag cgatctcttg tgcaatgatc tgcgcatacg tcaaaataat aatgaacaag
      781 acaatcgttg ctacataact tacgacatat tggactgtcg agttatcttc tccaactgtc
      841 atttttcctt ttgaatcaaa attgactttt tgtcgagtca aacttgcttg ttggctcaag
      901 ctagctactt cttctggtga aatgcctaat tgacttgctc gaagcatcga ttgcaagcca
      961 gtcagttgtt gttgtatcag tagttgtgta ctttgtccta atgaattttc gctataaagc
     1021 atcccattta tcttatcatt ctcagttgag actactaaat acgcatcgat tttttcttcc
     1081 gaaagctctt tttgtgcttt ttcttcagaa tcaactgctt tgaactcaaa atcatctgtt
     1141 ttgacttggc tcatttgctg actgatttgc tcattatcag ccaatacgcc gattttattg
     1201 acatcactat tttgttgagc aaagtttcct gctacataaa tgatacccag catgataaaa
     1261 gggactaata tcatgatcaa gaaagaaatc gacttaacat tttttaaata gacatctttg
     1321 gtaataatcc aaaacttacg catcgatttc acctgctttc aatttgaaaa tctcttccaa
     1381 agttggcggt tgctggttaa acataggaat gtagccgaac tgagtcgcac gagtaaagat
     1441 ttctttccca gcttcaggat cttctaaggt aatctctaac gtattgtctt catgaaggat
     1501 cacttttttc acgccttcga tttcttctac ttcctgttgg cttaaaccag attccaagaa
     1561 taacttcgta cggccaaatg attcacgaat ctcatgtact tttccattca aaaccatttt
     1621 tccgtttcgc aacatgatca aatgatcgca gattttttct acattatcca tattgtggct
     1681 agaaaaaatg acacatgaac catgtttttt caattccaat attccatctt ttagcaattc
     1741 agcatttacc ggatctaacc cgctgaatgg ctcatccaaa atgatcaatt taggttcatg
     1801 gattaacgtg gcgatcagct ggactttttg ctgatttcct ttagacaaag attttacttt
     1861 atccgtcttt ttccctttta cctgaaattt ttccatccaa aaatcgattt ttggttcaat
     1921 ctcttttctt gatttccctc taagttctgc aaaataaagc agttggtctt gaatcgttac
     1981 tttcggataa agtccccgtt cctccggaag ataaccgatg acatcataat ctttctcccc
     2041 aagatgatgg ccattccata acacttctcc ttggtcttgt gtcaaaaaat ccaagatcaa
     2101 acgaaaagtc gttgtcttcc cagccccgtt ttggccaatt agccccatga tttttccatc
     2161 ggggatttgg aaagagacat gatcgacagc tgtgtagttc ccaaaagttt taacaagatc
     2221 cttgacttct aacatttctt cactccttct tcaagaaatc acatatatcc ttagacacca
     2281 gacgtaagcg atttattatt taccaacaaa acgtaaacgc tctttttgtt gtacataaaa
     2341 tgataggaca ctttactttc tttgtaaagt gccctttcct cattcctgat gtaatttatc
     2401 aaatacgttt tgtgcgacat ttttcggcag tccgatttct tgtaattctt ctgtcgtggc
     2461 tgctgtgata tttttcaacg atttgaactc tttaagcagc attttttttc gttttggtcc
     2521 taacccttca atttcatcta atcgggaagc aaaactgttt ttactgcgta gctgccgatg
     2581 gaaagtgatt gcgaatcgat ggacttcatc ttggattcgc tggagtaaga aaaactctgg
     2641 tgaatttcgc tcaagcggta ctacagccaa atctataccg aatagcaact catttgtttt
     2701 atgtttatcg ttttttgcca tgccagcaat aggaatatcg attcctaatt gattttctaa
     2761 gacatctttt gccacatcta cttgtccttt tcctccatcg atcaagatca aatcaggtaa
     2821 aggcaaccct tcgcgaatca ctcgtgaata tctgcgatag atgacttctc tcattgatgc
     2881 ataatcatcc ggtccgttga ctgtttttat tttatatttc cgatattctt ttcttgccgg
     2941 ttttccatca atgaatacga ccatcgcagc cacaggattc gtccccatga tattagagtt
     3001 atcaaaagac tcgatacgta ttggggtcgg gatattcatc gcgtttccta aacgttcaac
     3061 tgctccgatc gtccgttcct gttttctggc aatcagatca aaccgttcat tcaaagcaac
     3121 tgaggcattc ttattcgcca attcaactaa ttttttcttt tctcctcgtt ttggttgcag
     3181 cactttcgta gctaacaaag cttctacact gggcttgtcg atcgtatcag gaatcaagac
     3241 ttctttagga atgaaatgct cattttcctg ataaaactga ccgaggtaag tcaagaaatc
     3301 ttcttctgct tcgttataga aaggaaacat tgaaacatct cgttcaatta gctttccttg
     3361 tcttacgaaa aacacttgga cacacatcca tcctttgtca atcgcataac caaatacatc
     3421 tcgatccagt aaatcagtat ttgtcatttt ttggcgagtc atgattgtct caatggcacg
     3481 gatctggtct cggtattctg cagctttttc aaactccatg ttttcagcag ctttttccat
     3541 tttctgatgg atctctgact cgattttttc gtgtcctccg tttaaaaact ttttcacttc
     3601 atcaacgatt ttcgtataga ctttcggatc tacgtcgaaa taataaggac acagacattg
     3661 tcccaaatga tagtaaagac aaggctcctt cgtctgacta ggtttgcatt ttctcagagg
     3721 aaataatcga tcgaggatct tctttgtctc attcgccgca ccgacatcgg gatatggtcc
     3781 aaaatacagt gctttgtctt ttagtacttt acgagtaatc accaaacggg gatatttttc
     3841 attcgttatc ttaaggaaag gataggtttt atcatccttt aacataatgt tgtatttagg
     3901 atcatttttc tttatcagat tgatctctaa gagcaatgct tcgatattcg attctgtaac
     3961 gatgtattca aagtcttcga tttcactaac aagccgttcc gttttcgtat tatggctgcc
     4021 agtaaaatat gagcggacac gattttttaa tattttggct ttgccgacat aaatgatcgt
     4081 tccattttta tctttcatca agtaacagcc gggctgatca ggaagtaaag ctaatttgtt
     4141 tttgattcgt tcgttcatct ggtactcccc ttcatattca attaaagtta ttatagcatg
     4201 tctgtcaaaa aaacaatcca gcctgacaac gtaattatac tcaaaaaata ttaatttgaa
     4261 taaccttaac tcaaccgcaa aaaaaccgag gacggaactg gattcagtcc caccctcagc
     4321 ttatttttgc tgtaagtttt tataaatatt gatcgatcat ttgacgtagt tgttcttttg
     4381 aatgaacacc aacagctttt tctactactt gaccatcttt tttcaacatt aatgttggaa
     4441 tgctcatgat accgaattgt tgaggagttt ctggattttc atccacatcc attttagcaa
     4501 ttctgaattc ttcttcatca tattcttgtt ccaactgatc caaaatcggt gcttgcatac
     4561 gacatggacc acaccaagtt gcccaaaaat caatcaatac aagtccttta tctgtttctt
     4621 cattaaatgt cttatctgta attacttgtg acatatcaaa gacctccatt tttaaaatct
     4681 atgattagta tagcatttct atacttggat agcgaaccat ctgcttacct ttagtaagct
     4741 ttacttaaat ttaacaattg tcgcaccatt tccgccttgg tttgccggtg caaactcaaa
     4801 gcttttgacg ctccgatggt ttttaagata atcagtaatt cccttgcgaa gtgcgcctgt
     4861 tccttttccg tggacaatag tcacttgagg ataaccagct agaatggcag aatccaaata
     4921 ttgatctacc tcacttagcg cttcttcgta tcgttttcct cgaaggtcca attgattcgg
     4981 cacgtgacta ctagattcag atcgtacagc atggataact cgttgtgtcg gctctttttg
     5041 aggagcaaca ggcgtcagct cgtcttcgga cacattcatt tttaatatac cgatttctac
     5101 ttgccactga ttcccgtttt tacgtaaaag cgtccctctt tggccgtaag tcgtaaccaa
     5161 cacttcgtct cccggtttca acgttttttg ttctttcgcc ttcttcaata ctttattttt
     5221 cttcaaagtt tcttcttgat gtagatttgc taattgggtc tttgcatcga tcaactgatg
     5281 ctctttcaca tttccttgcc caatctgctg ttgcatttta cgaatatcag caatgatttt
     5341 ttctgctttt tcttccgcct ctgacacgac ttcgtttgct tttttctttg ctttttccat
     5401 ttctttttca cgttcttcaa agaaatagct gtaagcttct ttcaaatcat cgtgaagttc
     5461 ttgtgcctca gaaacaaaat gacgcatttc cagatattcg gtctctgcca ttttccggcg
     5521 attctctaaa tctgtgatca tttcgtttaa atcttggctc tcatcattca tcaactgctt
     5581 cgcttcatca ataacttctg tatcaagacc taatcggcta gagatttcaa acgcattgct
     5641 tcgtccagga acgccaatca gcaaacgata cgttggactt agtgtatcca catcgaactc
     5701 catgctggca ttaatcgtat ttgcccgatt gtatccataa actttcaatt caggataatg
     5761 cgttgttgcc atcacataag cagacttttt accaagatca tctaagattg cgattgctag
     5821 agcagcacct tcttgcggat ctgttcctgc accgagctcg tcaaataata ccagactttt
     5881 ttcattgact tttgataaaa tatctacagt gttcgtcata tgagaagaaa aggtcgatag
     5941 actttgctca atcgactgtt cgtctcctat atccgcaaat acttcctcaa aaataccgat
     6001 ttgactttct tcacctgcag gaatcggtaa tcctgcttgc cccatcagtt gtaacagacc
     6061 taaagtcttc aacgtgatcg ttttcccacc tgtgttaggt cctgtaatga cgatggcttg
     6121 atagtctttt ccgattgtga tgtcattaga aactactttt tcctgatcga tcagtggatg
     6181 tcttgcttgt ttcaagacga catgattgtc ttcactgatt cccggcacga tggcttttac
     6241 ttctttacca aatcgtgctt ttgcattcat cagatccatt ttaccaatca cataagcatt
     6301 gtggatgatt tcttgacgat ggggaacaag ttcagcagaa agttcactta aaatacgttg
     6361 aatctcattt cgttcagcga tttggtattg acgcaaacga ttattcaaat ccaccacttg
     6421 ttttggttca ataaataaag tctgtcctga agcactttga tcgtggacta caccgccaaa
     6481 tacaccgcgg tattcttgtt tgactgggat aacgtagcgg tcatttcgca tggtgatgat
     6541 cgcatcactt aaatactttg catttttccc acggacgatc ccatctaact gttcacgaac
     6601 agcttgttca cttcgccgaa tgttctgtct tatgctcttt aactcaggag aagcgtcatc
     6661 agtcacgcgt ccgtcttcat cgattgcttc ctttaaacgg cggctcaaaa cagggatcgt
     6721 gactaactga tctgcccaca tatacaaacg tgcaaattcg atttcactgt cagaaagatc
     6781 atcaatgaaa cgattcaatt cgcttgtcgt agacaatact ctggaaactt gtgcaagctc
     6841 cactccattc aagtctgctc cgatctcgat ccgtttcata tggggacgga tattttcaag
     6901 tttaggtata gggatcccgc ctcgcagacg ttgcactttc aatccatcct cagtttcttg
     6961 aagccagttt gtgatcgttt gtttctcact cactggtacc aattctgcca gttcttcttt
     7021 tccttgtgca gtcactacaa attgtctgac catctgtttg actttttcaa attccaatgt
     7081 ttcaaggatt cggttattca ttttatcacc ttatctttca ctatgaaaaa agagctgtgc
     7141 cggacatacg ctacgcagcc ttcttaaata aactactctg tacaaaaaac gaaatatttt
     7201 ccagcactat tttccttaat tcaaaatatt cgttacccaa agctcataca tcttgttggt
     7261 aagaaatggc gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg
     7321 gaccacacta atggaacaaa ggcaagtaac tgcaaaatca aaaatatagt aacgtaagcc
     7381 atgattagtg acaggatgcc tcctgcaatc cagtctacct gtctcaagat cggcacatac
     7441 gtcaatccat ggacaaaaac ccccacaaat cgggtaagca gccagccaat tagcaaaata
     7501 atcagaaaag cagttcctgc ataaaaagct tcatctaggt gaaaagcaaa tacctgatca
     7561 aaaaatgcta actttgatgt tggggtgaca gctgggtatg gaatgtataa ttccaaatga
     7621 cttgctaatg gtttgtaaaa atgctgggca gcaataaatg acaatacata tccaatcgcg
     7681 aatatgattt gcaaagcaaa cccgcgacgt gctccagaaa aaaacgcaat tagcaataaa
     7741 aataaaatca gcaaactcag catgctacgt tcctttctct tctaactttt ttaaattttt
     7801 cttattttga taaaaggatc ttaattagaa gatttttgtt taatttgtcc ttttcgtttc
     7861 tcgtttaaaa tctgttgtgc ttccacatga tttttaatgg tatgatctgc ttgaccattt
     7921 tctttcaata cttcccgagc ttcgttctcg attgcttcaa tgcgtttcac acgattttca
     7981 agctctgtaa atttgatcat ttttttcttt aattctgctg tttcttcttc cagctccaag
     8041 attctttctt gtttcttcaa ttgatccgaa agcgcattga cagccatcaa gattgctgct
     8101 tgttcattat ccatttgtgg tgataattgt ttcaattctg ctaactgttc attgatcagc
     8161 ttcgttacga tatccatatg atgcttggtt tcccgtccga taatagtata tgtttggttg
     8221 gcaatcactg ctttgtagcg tgttttttcg tgcgccatac gaatcatttc ctcctatttg
     8281 atgaaagttt tatttagtgt accatgaagt tggttctttc accgttctcc acaaatattt
     8341 ttcacatact taactattat atgataaact ttaaaaaaat gctatgtctc gcaatctctt
     8401 agaaaacgaa aagtcgtgag ttgcggtata gttttatgta taaaaacgag gagaatttta
     8461 tgagcaacgt cattatcaaa gcaacaaacg accaacttag tcaaatgaaa gcatactatc
     8521 gctcttattt gttaaataaa cagattcctt atactagctt tgctgcaaaa aaagacggga
     8581 cgaccattac cgcttataca tccggaaaag tgatgtttca aggtacgaat gcagcaatag
     8641 aagctgataa atggggggag gcacaggata aacaagccaa gccagcaaaa acagcctctt
     8701 ctttgccaaa aaatatcagt caattatccg ttttaggtag tgatgaggtc ggaaatggca
     8761 gctattttgg tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat
     8821 tacgtaaact tggtgttaga gattcaaaag agttaaaaga tagccaaatc atccagcttg
     8881 ctgacatctt aaaaaaaacc atcccctata aactgcttgt cttatctcca gaaaaataca
     8941 atgagatcca accaaaatac aatgccgttc ggatgaaagt cgctttgcac aatcaagcga
     9001 tccatttgct gctaaaaaaa atcgagccag aaaaaccaga agcgattttg atcgatcagt
     9061 ttacgccgga agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa
     9121 aactgtactt cgttactaaa ggagaacaat atcatttagc tgttgctgct gcttctatca
     9181 tcagtcgtgc cagctttttg gaagagttac acaaagcatc agctgaagct ggaatcaccc
     9241 ttccatcagg agctggaacg aaatctgacc aagtagcggc aaaactattg gagaaaggcg
     9301 gcatgcctat gcttgaaaaa tatgccaaac ttcattttgc caatacagaa aaagctttga
     9361 agctgctaaa aaaataatgg aactgtgaca aaagtcatgt cacagtccct tgttccgaat
     9421 aaacggtgtt ctcatgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa
     9481 gctgtttttt gaaattgctc cttgtgacgc ggagttaaac ggcaggctca caacctcttc
     9541 cttaaacggt gttcaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg
     9601 aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt
     9661 gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta
     9721 tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact
     9781 tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca
     9841 attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct
     9901 tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct
     9961 tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa
    10021 accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata
    10081 tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca
    10141 ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg
    10201 actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga
    10261 taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt
    10321 ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat
    10381 gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg
    10441 ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata
    10501 atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc
    10561 gtagatccag actaagggtc aaaacttaaa atcagcttaa gtacgcaaaa aaggggctgt
    10621 aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat
    10681 atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt
    10741 cattctacaa acaattaa
//
LOCUS       DADJYG010000088        10587 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.090, whole genome shotgun sequence.
ACCESSION   DADJYG010000088 DADJYG010000000
VERSION     DADJYG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.090"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            102..269
                     /locus_tag="JZ382_001813"
     CDS             102..269
                     /locus_tag="JZ382_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAY2427995.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(331..2142)
                     /locus_tag="JZ382_001814"
     CDS             complement(331..2142)
                     /locus_tag="JZ382_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAY2427996.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2139..2504)
                     /locus_tag="JZ382_001815"
     CDS             complement(2139..2504)
                     /locus_tag="JZ382_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAY2427997.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2667..3062)
                     /locus_tag="JZ382_001816"
     CDS             complement(2667..3062)
                     /locus_tag="JZ382_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY2427998.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3080..4042)
                     /locus_tag="JZ382_001817"
     CDS             complement(3080..4042)
                     /locus_tag="JZ382_001817"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAY2427999.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4042..4254)
                     /locus_tag="JZ382_001818"
     CDS             complement(4042..4254)
                     /locus_tag="JZ382_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAY2428000.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4275..4973)
                     /locus_tag="JZ382_001819"
     CDS             complement(4275..4973)
                     /locus_tag="JZ382_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAY2428001.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4993..5535)
                     /locus_tag="JZ382_001820"
     CDS             complement(4993..5535)
                     /locus_tag="JZ382_001820"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY2428002.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5667..6671)
                     /locus_tag="JZ382_001821"
     CDS             complement(5667..6671)
                     /locus_tag="JZ382_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY2428003.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6668..7657)
                     /locus_tag="JZ382_001822"
     CDS             complement(6668..7657)
                     /locus_tag="JZ382_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY2428004.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7654..8460)
                     /locus_tag="JZ382_001823"
     CDS             complement(7654..8460)
                     /locus_tag="JZ382_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428005.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8626..9582
                     /locus_tag="JZ382_001824"
     CDS             8626..9582
                     /locus_tag="JZ382_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2428006.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9659..10177)
                     /locus_tag="JZ382_001825"
     CDS             complement(9659..10177)
                     /locus_tag="JZ382_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAY2428007.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10265..10414)
                     /gene="rpmG"
                     /locus_tag="JZ382_001826"
     CDS             complement(10265..10414)
                     /gene="rpmG"
                     /locus_tag="JZ382_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY2428008.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3294 a         2306 c         1804 g         3183 t
ORIGIN      
        1 tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt ttctcttttg
       61 ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa tttgtatctg
      121 gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt gcttctgtga
      181 aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa aaccgtaaaa
      241 aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat gaagactttc
      301 taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa gttgtgctat
      361 tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg ctgcagttag
      421 ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga tttcccctga
      481 agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat gataacttcc
      541 tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat tttttatgac
      601 aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca aatagcgact
      661 gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct gaatatcaaa
      721 tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt ctttcgtccg
      781 aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc cccatagtat
      841 ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga ttggatcacg
      901 ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc ctttttccgc
      961 ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct cattctgata
     1021 ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg ctggatgctt
     1081 tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt tctttggaat
     1141 cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt cttttttcag
     1201 atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct ccaggttttc
     1261 ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc ttcctcgagg
     1321 gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc ctgatggatg
     1381 atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg taaacaatcc
     1441 gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt taggcggtat
     1501 cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa catttttctt
     1561 tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt gaccgcttgc
     1621 taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat aaccatcttt
     1681 tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg gaaattcttc
     1741 ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag tagattgctg
     1801 atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg ttaccgtctg
     1861 ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc cctgtgttac
     1921 caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag aaacagaatt
     1981 cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac cattgggatc
     2041 gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc tttctgctac
     2101 acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc ctccatttcg
     2161 ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc ataaacaaaa
     2221 gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg cttcttttct
     2281 tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg ttctggtaca
     2341 tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca cagatctaac
     2401 tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat tctatagcca
     2461 tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt ttccttcttt
     2521 ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa aatgaacggc
     2581 acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt gattcggttt
     2641 ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt tccagaccat
     2701 ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg taatttattt
     2761 gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg gtcttttcga
     2821 atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc taaaatatgc
     2881 ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct cattccttct
     2941 cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg tagatagact
     3001 tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac ccataaaacc
     3061 atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca ccaatgacac
     3121 cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact tgcgggaaag
     3181 taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct gccgatactc
     3241 ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg caagctaaac
     3301 ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct tcttgagcca
     3361 tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct ttcagttttg
     3421 aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct gtagcgctag
     3481 atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca ggatccacag
     3541 tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt ccgccagcaa
     3601 cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga tcgttttctc
     3661 cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg atagcgaatt
     3721 tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc cagtttaagt
     3781 tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta cccatgccga
     3841 ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg attgattcaa
     3901 tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg ctccattttt
     3961 gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca cctaaatcaa
     4021 tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt caattcggtg
     4081 ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca aacggctatc
     4141 atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg cacccacata
     4201 gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct ccatctttaa
     4261 gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa aatacatatt
     4321 acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga atagcttgtc
     4381 tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag cccaccgaca
     4441 tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa cacaataaat
     4501 aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg aaaccctaag
     4561 ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga cagaacattc
     4621 gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat ataaatcaag
     4681 aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata cagtatcagt
     4741 gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac taaacgccac
     4801 cattcattga agtgaacaag ataaggacca tacattccgg cataataagg gatctcaagt
     4861 cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa agcataggtc
     4921 cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt catgaaaacc
     4981 tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc tctccggttg
     5041 ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat gaaaattatt
     5101 taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc tattgtagat
     5161 aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct ctgattgtgg
     5221 ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt cataaaacgc
     5281 catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt tttctgctcg
     5341 ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc ctatcacttt
     5401 tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt ctttttcctt
     5461 tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc gtaatcttac
     5521 tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag gaaaaaaaga
     5581 aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag acctagaaat
     5641 gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg tctgataaaa
     5701 agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt aggtacttcc
     5761 ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat ggaaccgaac
     5821 aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt cgcagcatga
     5881 ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc gcttgccaaa
     5941 gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc taatccttgc
     6001 gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc tcctagcgga
     6061 attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc accccagatt
     6121 gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg gatcgtgcat
     6181 agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa gattcgattc
     6241 atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc agctaaaaca
     6301 ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga aaagaaacct
     6361 aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact agaatctgcc
     6421 aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca agctcctgct
     6481 aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc caaagaagat
     6541 gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg cgtaccaatc
     6601 attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa cgtccaatat
     6661 atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg ccaatcaatg
     6721 caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct gtaaaatccg
     6781 ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa cgatagtccg
     6841 ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa acaggtccga
     6901 ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc aaccgcgttc
     6961 gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc aaagatattg
     7021 cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac aatactgcta
     7081 attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga tcctgctgca
     7141 gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta atagctacgc
     7201 cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa aaaccaaata
     7261 cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa aaatcagaag
     7321 ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg ttgattccta
     7381 gtaagcctga atctgctaaa ggatttcttg tcaccccttg catcaatgca ccgctcaatg
     7441 caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata tttcgtatga
     7501 tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa tctgcttttt
     7561 ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga acaaaacaga
     7621 ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct gctcttttgg
     7681 caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga caatcgttgc
     7741 atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag gtgtaccttc
     7801 tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg aagcatgatt
     7861 aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt cagtcaataa
     7921 ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat ccaagatcaa
     7981 gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt gtccaccaga
     8041 cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca acgcccactc
     8101 aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag ggtatcgtcc
     8161 gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt gtgctaataa
     8221 tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt tattgacaat
     8281 gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag tagattttcc
     8341 acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa cagacatgtt
     8401 ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta atctcgtcat
     8461 ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa catttcgtca
     8521 agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa atatcaggaa
     8581 taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa attaaaaaca
     8641 aaagtattca cattatctag catattgctg ctggctggag tactgagcgc ttgctccccc
     8701 tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa tagtactcat
     8761 acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa acggatcatc
     8821 ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc ccagtggact
     8881 gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt cccaacgatt
     8941 tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct gttgatttct
     9001 tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc gccaacttat
     9061 gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt cggcaaagct
     9121 ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc catcaagaaa
     9181 actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt gtgggtgacc
     9241 aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt atacgacgat
     9301 ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac ttctgactgg
     9361 tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat ccttgtgaac
     9421 agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa agcagtgaca
     9481 gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg acctattgcc
     9541 agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact aaaaaaagca
     9601 tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg catagatttt
     9661 attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa cgatgggcat
     9721 atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag tctgaaaaat
     9781 caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt cgccagatcc
     9841 gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca tagatatctt
     9901 ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact tccgcatttt
     9961 ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa ggaagtgaaa
    10021 gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc attgtatgtt
    10081 cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt ttgccgatca
    10141 gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct tatgatttta
    10201 gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga gaactttaaa
    10261 aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa tatttttgtt
    10321 tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta cgttctttac
    10381 aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt tttacaagaa
    10441 tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag attgctagtg
    10501 tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt ttacttctca
    10561 aacaaaatct ttttcttttt gtataaa
//
LOCUS       DADJYG010000089        10532 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.091, whole genome shotgun sequence.
ACCESSION   DADJYG010000089 DADJYG010000000
VERSION     DADJYG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.091"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(25..144)
                     /locus_tag="JZ382_001827"
     CDS             complement(25..144)
                     /locus_tag="JZ382_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAY2428009.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="JZ382_001828"
     CDS             complement(159..1343)
                     /locus_tag="JZ382_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAY2428010.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="JZ382_001829"
     CDS             complement(1599..1853)
                     /locus_tag="JZ382_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428011.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="JZ382_001830"
     CDS             complement(1878..3020)
                     /locus_tag="JZ382_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY2428012.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="JZ382_001831"
     CDS             complement(3080..4432)
                     /locus_tag="JZ382_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAY2428013.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="JZ382_001832"
     CDS             complement(4503..4847)
                     /locus_tag="JZ382_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAY2428014.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="JZ382_001833"
     CDS             complement(4857..5231)
                     /locus_tag="JZ382_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428015.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="JZ382_001834"
     CDS             complement(5244..5558)
                     /locus_tag="JZ382_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428016.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="JZ382_001835"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="JZ382_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="JZ382_001836"
     CDS             complement(8976..9638)
                     /locus_tag="JZ382_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428017.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="JZ382_001837"
     CDS             10255..10485
                     /locus_tag="JZ382_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428018.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DADJYG010000090        10481 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.092, whole genome shotgun sequence.
ACCESSION   DADJYG010000090 DADJYG010000000
VERSION     DADJYG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.092"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            33..656
                     /locus_tag="JZ382_001838"
     CDS             33..656
                     /locus_tag="JZ382_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428019.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="JZ382_001839"
     CDS             1106..2155
                     /locus_tag="JZ382_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2428020.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="JZ382_001840"
     CDS             2152..3033
                     /locus_tag="JZ382_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2428021.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="JZ382_001841"
     CDS             3036..3806
                     /locus_tag="JZ382_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2428022.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="JZ382_001842"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="JZ382_001842"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY2428023.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="JZ382_001843"
     CDS             5117..6280
                     /locus_tag="JZ382_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAY2428024.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="JZ382_001844"
     CDS             6397..6807
                     /locus_tag="JZ382_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY2428025.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="JZ382_001845"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="JZ382_001845"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY2428026.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9347..9664
                     /locus_tag="JZ382_001846"
     CDS             9347..9664
                     /locus_tag="JZ382_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428027.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9824..10060
                     /locus_tag="JZ382_001847"
     CDS             9824..10060
                     /locus_tag="JZ382_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY2428028.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="JZ382_001848"
     CDS             10057..10455
                     /locus_tag="JZ382_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAY2428029.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3655 a         1763 c         2221 g         2842 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa t
//
LOCUS       DADJYG010000091        10207 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.093, whole genome shotgun sequence.
ACCESSION   DADJYG010000091 DADJYG010000000
VERSION     DADJYG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.093"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            343..612
                     /gene="rpsN"
                     /locus_tag="JZ382_001849"
     CDS             343..612
                     /gene="rpsN"
                     /locus_tag="JZ382_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY2428030.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            627..806
                     /gene="rpmF"
                     /locus_tag="JZ382_001850"
     CDS             627..806
                     /gene="rpmF"
                     /locus_tag="JZ382_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY2428031.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            838..1215
                     /locus_tag="JZ382_001851"
     CDS             838..1215
                     /locus_tag="JZ382_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428032.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1236..1385
                     /gene="rpmG"
                     /locus_tag="JZ382_001852"
     CDS             1236..1385
                     /gene="rpmG"
                     /locus_tag="JZ382_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY2428033.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1409..2392
                     /locus_tag="JZ382_001853"
     CDS             1409..2392
                     /locus_tag="JZ382_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY2428034.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2422..2544
                     /locus_tag="JZ382_001854"
     CDS             2422..2544
                     /locus_tag="JZ382_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAY2428035.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2562..4085
                     /locus_tag="JZ382_001855"
     CDS             2562..4085
                     /locus_tag="JZ382_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAY2428036.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4109..4348
                     /locus_tag="JZ382_001856"
     CDS             4109..4348
                     /locus_tag="JZ382_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428037.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4541..5002)
                     /locus_tag="JZ382_001857"
     CDS             complement(4541..5002)
                     /locus_tag="JZ382_001857"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2428038.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5197..5415)
                     /locus_tag="JZ382_001858"
     CDS             complement(5197..5415)
                     /locus_tag="JZ382_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428039.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5533..7149
                     /locus_tag="JZ382_001859"
     CDS             5533..7149
                     /locus_tag="JZ382_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY2428040.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7390..8730)
                     /locus_tag="JZ382_001860"
                     /pseudo
     CDS             complement(7390..8730)
                     /locus_tag="JZ382_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9006..9161)
                     /locus_tag="JZ382_001861"
     CDS             complement(9006..9161)
                     /locus_tag="JZ382_001861"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY2428041.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9457..9789)
                     /locus_tag="JZ382_001862"
     CDS             complement(9457..9789)
                     /locus_tag="JZ382_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2428042.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3400 a         1616 c         2014 g         3177 t
ORIGIN      
        1 cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt catattgccg
       61 ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac taataaaagt
      121 gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga ttatctctat
      181 gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt tcatgttatt
      241 tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt aacgattacg
      301 atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa aaagtcgaaa
      361 attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa acgtcaggaa
      421 ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc aaatccgaat
      481 cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg aaagttcggt
      541 atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg agtaaaaaaa
      601 gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca tctaaggcga
      661 agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca tttgacgaag
      721 cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat aatggaaaga
      781 aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg aatcaaaatg
      841 gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga aacgacccag
      901 caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat tattactgaa
      961 acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta ctatgaggac
     1021 caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga gtatgacgag
     1081 gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa agaaaattta
     1141 gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat tcaagcatat
     1201 cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt attttggaat
     1261 gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat aaccctgaac
     1321 ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt aaagaagtga
     1381 aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg tttctgggtt
     1441 tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac cctaccccag
     1501 ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc acaaattatt
     1561 agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt gcagtcttcg
     1621 cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac atcaacaacc
     1681 aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa tcattcaaac
     1741 acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat tgactgttat
     1801 tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc aacttgtcat
     1861 ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac taaaaaaatt
     1921 acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca cacatagtga
     1981 aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct tagtttttga
     2041 aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc atcaagtaca
     2101 tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt ttttactcca
     2161 atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta aaggattgat
     2221 tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac aggttaattt
     2281 tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa aaatgttgga
     2341 tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat aatgataagt
     2401 agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata tcgacgactt
     2461 aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga acataaacga
     2521 aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa atcagtgtag
     2581 gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact aatgatgcaa
     2641 aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat ccaatgtatg
     2701 atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt ccagcgggat
     2761 cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat gatgctgatg
     2821 tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa gaaagttgga
     2881 agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg ccaggtagtg
     2941 aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat cctcatacgt
     3001 gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag ttagttaaat
     3061 tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg actaaactga
     3121 aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa agttttgtga
     3181 cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa gttcctattg
     3241 ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta aaagagtatg
     3301 ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat aaaattgcaa
     3361 aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt gaaagtctta
     3421 caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac aatctaaaag
     3481 cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa gaagcgaagg
     3541 atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat cgcgaattga
     3601 gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga attttggatg
     3661 aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag tacaaagatt
     3721 attatactac tggctataag acagatattg atactatcaa cattaaagac aacaccattg
     3781 attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga tacaaaattt
     3841 tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat gatgcaggtg
     3901 ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt aaggcagagc
     3961 atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa ttgcataatt
     4021 ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa atgatggctc
     4081 attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt ctatggattc
     4141 caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat taaatgggag
     4201 tacattagct gatattgctg caactttaca tgttagtatt gacacagttc atagagtatt
     4261 aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta gacgtttagc
     4321 aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca tttttccctt
     4381 atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg caacacttta
     4441 tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct gttattcagt
     4501 ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt cgattttggc
     4561 aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca agggcatgag
     4621 gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa tgattggaga
     4681 attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata ctgcttaaaa
     4741 gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc tttcctgcta
     4801 aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca gttttgccag
     4861 ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat tttggacggc
     4921 catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt tttgtatgtc
     4981 ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt gagaggggtt
     5041 ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta attgaaaagt
     5101 ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga aaatcgacaa
     5161 ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa cgtccgcatt
     5221 tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt ttttcttgtt
     5281 tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc caattatact
     5341 aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta tagtaagatg
     5401 acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact tatactagtt
     5461 ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc aaggtataga
     5521 aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct tggttcagga
     5581 aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat tgctgtcatt
     5641 gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa aggttttaaa
     5701 agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac attacgcgaa
     5761 gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca aattgttatt
     5821 gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt agctaaaaac
     5881 cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat agtagtggat
     5941 gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag aaaagctgaa
     6001 ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca attcgagtgt
     6061 tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat ttacaaaatt
     6121 aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat taaaggtgaa
     6181 gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac ttcctattca
     6241 gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga aacagaagaa
     6301 tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca aaaatttgcc
     6361 catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt tctttggttt
     6421 gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa aagtattgaa
     6481 tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat tatacaaaaa
     6541 aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa taaactagtt
     6601 tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg ttgtttaact
     6661 gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa aaagtctaat
     6721 atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa gataggcgat
     6781 gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt attctctata
     6841 gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg aattgatggc
     6901 aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa ttatcatcct
     6961 gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt taaagaagat
     7021 aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg tcaaacgagt
     7081 atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac agattactac
     7141 atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat tattattaat
     7201 aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag aagcggtgac
     7261 aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc ggttagatta
     7321 cttattttgg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat aatcgagata
     7381 ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt tagtcagttg
     7441 ggtagtattc ttagttttct taaaatattt tcctcgatat aaaaagattc gttctcggta
     7501 gttttgaaag tttctatagc cataggcaat tcgttttagt actttgatat ggttattcaa
     7561 acattctata gggccgttgg aatagggaac gcgcaacgct cgtttgattt ctgtttggta
     7621 tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt agtcttcttt
     7681 taggagcgcg acgaattcag gaacatcttg tcttctgata acggaaagaa aatcctggta
     7741 gacttcgtag cctcgcttta aggaatcatc ataagataat agccgatcga ctatctctgc
     7801 ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgacctt tcaattccca
     7861 cgcattcttt tgaagtagtt tccagtattt ctttaagtgc ttcgaccgac gatccccttt
     7921 tttaaaggtc ttcattactt gaattcggtg gttggtaaaa gcccgaccaa tgtgttggct
     7981 aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca ccaatgtgcg
     8041 atagggtgta tagaaatctg aaaccactag acgcacttgt tcacggactt ttatagggta
     8101 gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat caatcagttg
     8161 atgggtcttg ccgtccatca tgacaaagct catggctccc gagacatttt tcactgattt
     8221 gaattcatcg aaacacacca cttctggtaa cagctggctc aaagaaatgg ttttaggccg
     8281 aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac gtgcgatcaa
     8341 agacatagaa agaggttcgc ttaatagctc aagaatggct tgtttgactc tgcgagcaat
     8401 ggagcagcga cgatcactaa ctgaatcttc tgctaaaaaa gtctttccac agtgtttaca
     8461 tttgaagcgc tgtttgttaa tccgcagaat ggtcttgtag ttactgacat cattcaataa
     8521 gatggatact tttttccagc cctatttaat gatcgtttgt tcattttgat agtggcgatg
     8581 aaagcattcc tccggttgat aggtcaatgt gccatcaaaa acaaaacatg tttcgtcatt
     8641 gattcgctct ttgcgaaagg catcttcatg aaagataaga tttaggtcta gaatatctag
     8701 taactctttg gtaaactctg aataggacat cttgaaacac tcctttgaat ggttttcgtc
     8761 gactttattc taaaggattt taaggtgtct gtttgcgttt attagaaaaa agaaaaagta
     8821 ttggcaacag aaggcgtcac caacactaga tattatagat cccttatttt cttcgcttca
     8881 aattgtcaag gagtcatcat aaattttgta tggattcttt tgtttgggta gtagtcttct
     8941 aaataatctt ggattaacac cttcatctct attttacttt tatatttgtt ggagtcgatt
     9001 aattctcatt tgattgatcg atgaaacgct tcaattactg cattgtcaac aggagtccca
     9061 gtttggctca ctgaatgtgt gatgtggtgg tcgttcaaca gctcttgata aataaaagac
     9121 cgatgctggc tacctctatc ggaatgaatt accgtaggca attctcctta ataaatttgt
     9181 aatggacgga cgacaatctc ttttttcatg tgtgaatcta ccatatagta acgtagaata
     9241 cgagttgcta agtcaatata ggtggacaaa tagaggcgac cgtcattaca tggaatgtaa
     9301 gtaatatcca tcacccaaac agcatcaatt tggttctgtt caaattatcg attcaacatt
     9361 tttttaccat ttctttgatt gttttttctc taccatgaga agatttgcgt cgatgaaatc
     9421 ctcttgtgca taaattcatt tcacgcataa gaacagctac aacacgttta ttgatttcga
     9481 ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc gtatcgatct ttgtgttcgt
     9541 agaacactcg tcttcctctt ttagtcgttt attttcctgt tcaagaatgt agaaatctgc
     9601 tttatcaggt aatctttttc cttgtccagc aaaagcattt ttcccgtatc gacgatattc
     9661 tgaaacccat cgggaaattg ttggacgaga gatattgtat tcttcagcaa ttttcttggt
     9721 agttactttt ttctcaagga tatcctgtga aatctttagt ttaaatttac gatcatatgt
     9781 ttttctcaat agagttcatt ccttccggta acgtaactga tagtcaagaa aagtgtatca
     9841 tagcccaact tagtatcagg gtttgtcaac taaactgtgg aagttgatag ataagagttt
     9901 ttaacaacca aaattctctt gactattttt gaaatcagac aaattttgat tggacacata
     9961 gttgaataaa tctaccgtcg ttacggaagg taaatcgcat gcttttcaat tctagaggag
    10021 aagtcctcgt tgaagtacca tagcccagtt ctggattcga cctcctgccc acttcatacg
    10081 taaataaagt aattttagga gtgagactgt gacaaaagtt atttttaagc taaaaacgaa
    10141 ttaaaaaacg ttgataatta aaatcattcg ttttagattc tatgaatcat tatagaacgc
    10201 tgtaaaa
//
LOCUS       DADJYG010000092        10195 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.094, whole genome shotgun sequence.
ACCESSION   DADJYG010000092 DADJYG010000000
VERSION     DADJYG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.094"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            120..626
                     /locus_tag="JZ382_001863"
     CDS             120..626
                     /locus_tag="JZ382_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAY2428043.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="JZ382_001864"
     CDS             643..1542
                     /locus_tag="JZ382_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAY2428044.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="JZ382_001865"
     CDS             1629..2513
                     /locus_tag="JZ382_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY2428045.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="JZ382_001866"
     tRNA            complement(2705..2776)
                     /locus_tag="JZ382_001866"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="JZ382_001867"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="JZ382_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY2428046.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4373..5410
                     /locus_tag="JZ382_001868"
     CDS             4373..5410
                     /locus_tag="JZ382_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAY2428047.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="JZ382_001869"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="JZ382_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY2428048.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="JZ382_001870"
     CDS             6562..7752
                     /locus_tag="JZ382_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY2428049.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="JZ382_001871"
     CDS             7856..8611
                     /locus_tag="JZ382_001871"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY2428050.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="JZ382_001872"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="JZ382_001872"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY2428051.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3566 a         1886 c         2112 g         2631 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cagat
//
LOCUS       DADJYG010000093        10176 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.095, whole genome shotgun sequence.
ACCESSION   DADJYG010000093 DADJYG010000000
VERSION     DADJYG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.095"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(247..759)
                     /locus_tag="JZ382_001873"
     CDS             complement(247..759)
                     /locus_tag="JZ382_001873"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY2428052.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="JZ382_001874"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="JZ382_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY2428053.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="JZ382_001875"
     CDS             complement(3212..3646)
                     /locus_tag="JZ382_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAY2428054.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="JZ382_001876"
     CDS             complement(3656..4444)
                     /locus_tag="JZ382_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2428055.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="JZ382_001877"
     CDS             complement(4441..5382)
                     /locus_tag="JZ382_001877"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAY2428056.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="JZ382_001878"
     CDS             complement(5559..6146)
                     /locus_tag="JZ382_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAY2428057.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="JZ382_001879"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="JZ382_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAY2428058.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="JZ382_001880"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="JZ382_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY2428059.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="JZ382_001881"
     CDS             complement(9063..9998)
                     /locus_tag="JZ382_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2428060.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2818 a         2309 c         1763 g         3286 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    10141 aattgtattt ccgacgaaag aaaacaaagg agtgta
//
LOCUS       DADJYG010000094        10112 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.096, whole genome shotgun sequence.
ACCESSION   DADJYG010000094 DADJYG010000000
VERSION     DADJYG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.096"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            144..992
                     /locus_tag="JZ382_001882"
     CDS             144..992
                     /locus_tag="JZ382_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY2428061.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1137..2078
                     /locus_tag="JZ382_001883"
     CDS             1137..2078
                     /locus_tag="JZ382_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAY2428062.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2068..4239
                     /locus_tag="JZ382_001884"
                     /pseudo
     CDS             2068..4239
                     /locus_tag="JZ382_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4351..6522)
                     /gene="copB"
                     /locus_tag="JZ382_001885"
     CDS             complement(4351..6522)
                     /gene="copB"
                     /locus_tag="JZ382_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAY2428063.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6542..8728)
                     /locus_tag="JZ382_001886"
     CDS             complement(6542..8728)
                     /locus_tag="JZ382_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAY2428064.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8728..8937)
                     /gene="copZ"
                     /locus_tag="JZ382_001887"
     CDS             complement(8728..8937)
                     /gene="copZ"
                     /locus_tag="JZ382_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAY2428065.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(8950..9390)
                     /locus_tag="JZ382_001888"
     CDS             complement(8950..9390)
                     /locus_tag="JZ382_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAY2428066.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9464..10003)
                     /locus_tag="JZ382_001889"
     CDS             complement(9464..10003)
                     /locus_tag="JZ382_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2428067.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2846 a         2149 c         1820 g         3297 t
ORIGIN      
        1 ccattacaat gaaataaata gcgattttat gagaaggtga tgtgaaaatg gaagaaaaaa
       61 tagaagaatc caaacaaaaa atcgatcagt tgatataaga attagaaaaa gcggtcatca
      121 gcaaacgttc aactatccaa ttgatgatcg tggccctatt agcaggtggt catgtcttat
      181 tcgaagatat tcccggtgta ggtaaaacgt tactggtcaa agcactagca aaagctgtac
      241 agggaacctt cagcagagtc caatgtactc ctgatctgct gcctagtgat attcttggtt
      301 tttccgtata caattcacaa tcaaaagagt ttgaatttcg tccgggaccg atctttacca
      361 cgatcttgtt agcagatgag atcaatcgta cgactcctcg tactcagtca gcacttttag
      421 aagcaatggc cgagaatcat gcaacgatcg acaacaatac ataccctcta gacaatcatt
      481 tttttgtctt agctacgcaa aatccaatcg agtacgaagg aacatatcct ttgccggaag
      541 cacagctcga tcgtttttta ttccgactac aaatcggtta tccttctttt gatgacgaac
      601 ttttgctact tttggataaa ttcgaaaaag tgttggataa cttaaatgaa gtattgaaca
      661 gttatgaaat agaagaaata aaaacaaatg tggcgaatgt ctttgtcagt cccgaagtgg
      721 catcttatgc cttgcagtta gtgtcagcaa caagaagtca tgctgccatc cagcttggca
      781 tcagtccacg aggaagcctt tcctttattc aggcagcaaa agcttatgct ttaatccatg
      841 ggagaaatta cgtcacgccc aaagatttgc aggatttagt tccttacgtg ttttcccatc
      901 gcctgatttt ttacgatcgt tcattgaagg aagacgaaaa gaaacaaatc attcatactc
      961 ttgttgcaca aattcctatt cctgtgaggt agattgaggt agacaatgga aaaaatcaaa
     1021 gattatttca ttaattttgt gttatttctg gtatatttca tcattgtgct ttttgctctt
     1081 gtattcaaca attctttagg ctggttcttg ttggcctttt ttactcttta tgtatcatgg
     1141 agcttgattt cattaagcag ctttctaaag aaactttcgg ttacttgtac attgcagcca
     1201 ctcgtttatc gcaaaaaaag tatgcaggtt attttttcca tagcaaaaaa gagaactttt
     1261 tctttacctc tccctcgttt acaaatcggc ttcccagcaa tctttgcaga aaaaaacaag
     1321 agattttgat tcttacaaat aaaccgcaag aagtccaatt gctttggcag ccaaaagaaa
     1381 gaggatgcca tgaaacatta actgtcattt ttacagccta tgatagtcta ggacttttca
     1441 gaaaaagtgc aaaaagagag atctcttttc ctgtgactgt tctgcctgct ttttgtaaga
     1501 ttcaagcaga aaaattacag agagttctgg aaaaaaaaca gcagctgaac gcgtaccaaa
     1561 gaggtcttga ttttcgggaa catcgcgctt atcgtccagg tgattctttc aaccgaatcg
     1621 attggaaatt gtctgctcat actcaagagt ggttataccg tgaatacgat tatcaagaag
     1681 aagaatgttc tcctcttttt tgcttttggg gtttgcccag tccaaaattt gaacctctgc
     1741 tagacttgta tttttcttta tggcatttga gaaaagaaaa gcagccggaa cttctgatat
     1801 taggcaaccg cgcctttcat ggcaaagacc ccggtcatac ctattttgct tctttggaag
     1861 ctggcgatga gaaagctttt ttcgcacact tgaaaaaata tgcaaaaaaa caaatcgtcc
     1921 tctttgttcc tgagaattct cctgaagtct cagaagctat tgaaacattg tcaaaagatc
     1981 gaacggttta tcttgtttat ttcgtagaaa aacaactcat ggtccaagaa aaagacaaga
     2041 tatgttcatt gccaggaggt gaatggattg acgattaaac gggaaaaatg gctgcgtggc
     2101 gtttactcct ttgtgatact aagtgtaggt gctccgatat ttgtagtagc taatcaattg
     2161 caaaacagct accttcttat cctaactatt ggtgtcctat gccttattgt ctgctgtatt
     2221 cctaaaaaat gggtacgttt gctgctttct ttgccagtaa tgataggttg tttgttttta
     2281 tattttcctt cgaagacatt ttccatatta tggatcttac agtttttttc gtcagtcaca
     2341 gaggaattct tccatctatc ccaaggtaaa ctttattatt tgccagaaaa gacagctttt
     2401 tttctcataa tgttgctgat ctatttattt attacattga ccgtggatta cgactattgg
     2461 tacgcaccct ttctttcttt gatggcttac ttcatgatgc ttaccgtttt tcggaaaaaa
     2521 gatttactgt acgaaatgat tgcggtagta actgttttgt tcgtgtatct ggcagcaagg
     2581 caatttttct ccatccggtt gttgtcggga aacactcgat tccagtcatt gactacagtt
     2641 ctgcttattt gtttcttaac ttttagcgct atcttgcctt tatatcttcc gaaagtacac
     2701 caatctctcg aggaaacgag tgaaccgatc cgagaatatc ttaatgatga agggctttat
     2761 gacacactcc atgagtatca attaaccgga agtgcaagaa caggcttcag tgagaatgat
     2821 gaacagcttg gtggaccttt atatgataat ggcgaagttg tttttacagc gaaccaaaaa
     2881 ggaaatggct attggaagat cgaaaataaa aatcattaca ccggttctgg atggatagaa
     2941 gatcaggacg cacccatcca accaatccaa gaattccctg ttgacatcac gagctaccaa
     3001 accgttatag aagacaaaga aaacattaca ttgaccctaa aaaaacaagt tttcttacct
     3061 tatccttatg gaaatactac cttgactgat ttttcattag ctagtggaca aacgctttac
     3121 tatttgcctg aaagccagcg attcattcct gtcccgaatg aatcgacacg ttctgcaact
     3181 ttacaaacag aaaggttagt ttttacgaga gaagaattaa aaagcagtca gttaaaagct
     3241 gatgatttag aaaatcagca gacaaatctt caattgccaa aaagtttgcc taaacgtatt
     3301 gaggacttgg cggtcgaatt gaccaaaaat gaagcaactt taattcgtaa agtcgaagct
     3361 gtctaacagt atttaaaaac aagcggaaat ttccgctatt ccaaaacgga taccgggcat
     3421 actccaaaag aacacgatta tgtggattat ttcctctttg attcaaaagt cggctattgt
     3481 gacaactttt caacttctat ggtcgtcctt cttcgaacac ttggtatccc agctcgctgg
     3541 acaaaaggtt ttacaaatgg gctcagaaca ggcacagata atcaagggaa tgaggaatac
     3601 agcattctca atagtcatgc tcattcttgg ccagaggtat actttcctgg atttggctgg
     3661 cttccatttg aaccaacccc ttcatttgcc aatccaatgg gttcacaacc agttgctgaa
     3721 acaaccgaaa ccgtccagcc tgctatcgaa caaccaaatg aaagcgaaaa tatggcagaa
     3781 actatttcca gtgagagatc tgaggtcatt ccatcagaag aaacaacact cacaccagct
     3841 tataaaatcg tcttattcag ccttggagtg tgtttatccg tcttgttgtt ctttctattg
     3901 ttattcagaa aatctctgcg ctggacaagt tatcttatct atcttcattt ttttgcttct
     3961 actaatacga ttgcatatca aaaacttctt aaccctttgg aaacattggt accgcgcaaa
     4021 cccgcagaat ctttgcagga atatagcctg cgaatagaac aagaattcaa gctagatctg
     4081 cgtttcattc gattaactac tcaatatgaa gcagaaatat atggacgaca agcagatccg
     4141 gtaaaattcg ataaaaaaac ctatttagaa ataacgcata taataagaaa aatggtatac
     4201 aaaaaaattc tgacaaaagg gagccgcttc aaaaaataag ataaaatttc tggagaatct
     4261 acttctggaa tgccgagaac cagctgattg agtatcgatt actcgaaaaa aaggttgtga
     4321 cacgtttttt gtcacaacct tttttcatta ttatctgatt ttcaatgtca aagcatttag
     4381 agctacaact accgtactta acgacatcaa gacagcacca actgctggac ttaatatgat
     4441 accaattggc gcaagaattc cggctgctag cggaattgca agaatattgt aacctgcccc
     4501 ccaccagagg ttctggatca ttttctttcg cgtttgcttg gctagatcaa gaaaatgcaa
     4561 aatatctttt ggatcgctgt ttgtcaatac gacatccgca gaatcgattg cgatatctgt
     4621 ccctgcaccg attgccatcc cgattgaagc acgggctaaa cttggcgcat cattgatccc
     4681 atcacccacc atgattactt ttttcccttg gtcaaggtat ttctggacaa cagcttcttt
     4741 atcatctggt aataaaccgc cataatattc ttcgatccct aaataatctg ctactgcttg
     4801 ggcagcgttt ttattgtcac cagtaagcat gacaggagtg atttttcttt ctttcaatgt
     4861 tcggataaat tcttttgctt ctgtttttac tacgtcacct aaagcaacga ttgcagctag
     4921 atgattttct aaaatcagga agctgatggt atttccttgt tcttggtaag gtttcaataa
     4981 tgcttgttct acgtttaatc cgagacgctc aacttctttt tcattaacta ttttcacttg
     5041 ttgttttttg actgttgcct ctagcccgac tcctgaaaga ttttttagat tttcagcttg
     5101 ataaggcttg atttcatgtt ctttcaaata attcataatg ccaacagcca atggatgatt
     5161 ggcattcgct tccaaggctc ctagatattg aaggatttca tttttattga acgcatcatc
     5221 aagtacttcg acacccgtta cagtaaattt tccttctgtt agtgttcctg ttttgtccag
     5281 catgatcaca tctagatcat tggcttgctc cagtgcattg cggtttttca aaagtaatcc
     5341 gtttttagct gctattgatg tggaacgagc aacaactaat gggatagcta aacctagagc
     5401 atgaggacag gcgatgataa agactgttac catacgttct aatgcattag gaagatctgt
     5461 caagaacaac caggcgatga atgccaggat acccacaatt aatgcgacat aaaacagcca
     5521 ttttgccact ttgtcagata atgactctag cttggatttt tctccttgtg cttttcgcac
     5581 catttccata accttcgcta gataaccatt ttcaccagtt ccagttactt cgatttcgat
     5641 cgttccatcc ccattgatag aaccgccaat cactttgtca ttggcttgtt ttttgattcc
     5701 tcttgattcg ccagtcacag ctgattcgtc aacaattgtt tcacctttca agattacacc
     5761 atctgttggc attttatcac cagaccggac gatcaaacga tcaccttcgt ttacttcttt
     5821 caatgaaact gtctcttctt ttccatcttt cgtcaagcgt ttcactgatt ctggcagtaa
     5881 ttctgctagt ttttgtaaag cattgctggc attcgacaca gcattcattt caatccagtg
     5941 acctagtagc ataataacga tcaaagtagc caattcccag aagaaatcca tgacgtgtgt
     6001 atgtggattg agcagatttg cgataaacga atagacacta taaatatatg caactgtaat
     6061 ccccatcgcg atcagtgtca tcatagcagg acttttttgt ttcaattcca tcttagctcc
     6121 acttaaaaat ggctgaccgc catagataaa tagtatagtt gctaagatca atacgagcca
     6181 atcagaacca ggaaacgtta cttgaaatgg aaattccatt cccatcattg gtgaaaaaag
     6241 aatgattggg atagctagga taagcgatag ccaaaatttt tgtttgaaat tccccatgtg
     6301 catggaatga tccatgccgc cgtgttcgtg atccatgtgc atcgaatggt ccatttgttt
     6361 gctgtttgct atatctccat ggttatgtcc catgtgcatg gaatgatcca tctgactatg
     6421 gtcatgtttc atctccatct gtttttcatg agattctgta ctttctttgt ttactacttc
     6481 ttttttatgc tcatgttttt tctccatatc gttcccattc atttttactc catcccttct
     6541 cttacttttt catgtgtcga tttaagcaca gggagttcaa caatacactg acggaactaa
     6601 acgccatcgc tcctcctgca atgattggat tcaaaaagcc tagtgcggca aaaggaatac
     6661 caatcgtatt atagacaaaa gcccagaaaa gattttgttt gatttttctc aatgtcaacc
     6721 tagacaactc gattgtttgt gcaatggatg ccaaactgct attcatcagc gtcacatcgg
     6781 ctgtttccat cgcaatgtcc gttccacttc ccattgccat accgacatca gctaatgcta
     6841 aggccggtgc atcattgatt ccatcgcctg ccataccgac ttttttgcca tctttctgaa
     6901 gtttttctac gtaggctgct ttatcctctg gaagtacttc agcaaagata tgctttggat
     6961 caataccaac ttgtttacca atcgtttcgg ctgccagttt gttgtctcct gtcagcataa
     7021 aaacatctaa tcctttattc tggagctgct tgattgcttg tttgacttct agtttgattt
     7081 gatcagccac ggcaatcaac ccgatcactt gcttttcatc agctaaaaac atgactgttt
     7141 ttccctgctg ttctaattct aacgcgtgtt cttggtattc atcaaatgat aggttcaatt
     7201 caattaatcg tttacgtgta cccgcaaaat agcgtactcc gttaatcgta ccgctgattc
     7261 cagcacctgg gtgggctgta aaatcaatca tatcgtatgt tgctgcttct tgcagttttc
     7321 cgtattccac gattgcttta cctagtggat gttcagaagc ttgttctaaa gtataaaaaa
     7381 tagataggac ttctttagaa ccgactagat ctgtaacttt gggctttcct tctgtaatcg
     7441 ttccagtttt atccaaaaca atgctgtcta agtgagcagc tgcttctaat gcttctccgc
     7501 ctttaattaa aattccattt cgtgcaccaa cacccgttcc taccataata gctgttggag
     7561 tcgctaaacc taatgcacat ggacacgcaa tgactaatac agagacacta tggatcaatg
     7621 cgagttgcca atcaccagtg atcaaacctg tagctatgag cgtgataaac gcgatgacta
     7681 atacgatcgg aacaaaaatc cctgagatcc gatctgcgat tttttggatt ggggctttgc
     7741 tcccttgagc atcttccacc atttgaatga tttgcgctaa tactgtctgc ttgccgactt
     7801 gtgtaacttc catgtgaagt agaccattcg tattgattgt cccgccaaag agcgtgtcat
     7861 ccggattctt ttctacagga agattttcac cagtaagcat actttcatct atcgcactgc
     7921 ttcctgaaat aattcgacca tctgctggaa tctgctcacc aggacggatg actagttgat
     7981 caccaacgac tacttcttca atcgctaacg tttcttcttt cccgtttctt atcacttggg
     8041 cggtttttgt ttgaagagac atcaattgtt tgattgcatt acctgttttc gtttttgctg
     8101 tatgttccaa atatttgccg agcaaaatca gcgtgatgat catactgctg ctttcaaaat
     8161 aaagatcttg ggggtgtcca cggaaaaatc cgttgtaaat gctcaatgca aaggcagccg
     8221 atgttccaat tgctaccaat acatccatat ttggtgcttt cgtcttgatt gcatggtacg
     8281 cacctttata aaaacgagca ccgacataaa attggacagg agcagacagc actaactgga
     8341 cgattggaaa atgaaaaaag tgaacaatcg ccgcatggct tcctaacatc atagcaatca
     8401 ttgacaacat caacggcaaa gtcaaaaccg tactcaatat aagatcgaat agcatttttt
     8461 tcaaatacgc ttgcttttct tctgcaattt tttgtttgtg ggcttcatcg tacaaaattg
     8521 caccatagcc gatgttttct acgctttgga ttaatttttc agtggtcgtc ccttcatatt
     8581 tgacacttgc tttttcagtg gccaagttga ctgtggcatt gaccacacca ggttgtccgt
     8641 tcagctcttt ttcgatccta gcagaacaat ttgcacaagt catacctgta atgacgaacg
     8701 tatccatttt ggtttctgct gttgccatca aatcacctct gctttataac ccaatccatt
     8761 gattgcttga caaatctttt cggattgaac ttttgtttca tcaaacttga ccgtaccgtt
     8821 tgcttttttc aaatttactt tcactttttg cacgccgtca agtgcgctta ctgtttcttc
     8881 tactcttgct gcacaatgat tgcagctcat accttcgatt gaaaattgtt gtttcatttt
     8941 ttcttcctcc tattgatgtt gtttacattc acattgacct gggatacagt tacattcgat
     9001 cgtatctact ggttgctttt gttccagttg tttcaaaatc tcattgatat cttcttgagt
     9061 caaagtggct tcttctacta gatccgcaat cgtctctcca atcctttttg cacagatatg
     9121 ggaaaaaaga ttttccgtcg cactgcgaac tgtttccatc tccgagacag ctggataata
     9181 aatgaatttt tttccttctt gttctgtcca gatcacttct ttttttacca aacgacccag
     9241 caaagttttg atcgttgctg gtttccaatc catcgaatca gctaagatct gcgtgatttc
     9301 ttgggctgtc gtctgtccaa gtgtccaaat aacacgcatg atctcccact ctgaatcact
     9361 gatctttact ggcgttttga ctttttccat ttatttcaca cctttcgttt acaaatgtaa
     9421 tcattatctt atgaatacag attacacttg taaacaaaaa ctgtcaatta tttttgctct
     9481 ctattttcaa tttagttaaa aattcttgtt tctctcgtct tgttttaagg atgatcagtt
     9541 tatttcttgc attagctact tggatcagct gctgtatttt cggttcattg ttttcttcaa
     9601 aatgccagat aaaagataaa aattctaagt attccctgtt tagtttctcc gaaaatgatt
     9661 caggcatatc tggccgagat tttttgttta tctttctttt tagagaacgc caaactactc
     9721 ggtagatggc gagataacgc ggcactttaa gccaaatgat cttatctgct tctttaatcc
     9781 tttcctctaa tgtagaagta taatttcctt caacgatcca tgacggatta gataccataa
     9841 acaattgctg ttcttgcaaa aaccactttt ttgcctgaat actgtagtct gttgtatgcc
     9901 ataaaaggtc tagcggcagt agcggaaagt cggtgatttc tttgatcttc cttgcgaggg
     9961 tcgattttcc cgacccactt tgtccaatca cgatgatctt cattcttctc cctcacttct
    10021 ctttggaatt tggttcgatc ataccactta taacgccgca aaaaaacaag ggactgtgac
    10081 aaaagtcgtg tcaaagtccc ttgtttcgaa ta
//
LOCUS       DADJYG010000095        10090 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.097, whole genome shotgun sequence.
ACCESSION   DADJYG010000095 DADJYG010000000
VERSION     DADJYG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.097"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            162..392
                     /locus_tag="JZ382_001890"
     CDS             162..392
                     /locus_tag="JZ382_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAY2428068.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="JZ382_001891"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="JZ382_001891"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY2428069.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="JZ382_001892"
     CDS             1803..1979
                     /locus_tag="JZ382_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY2428070.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="JZ382_001893"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="JZ382_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY2428071.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="JZ382_001894"
     CDS             2474..2941
                     /locus_tag="JZ382_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428072.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="JZ382_001895"
     CDS             3026..3910
                     /locus_tag="JZ382_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2428073.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="JZ382_001896"
     CDS             4027..4260
                     /locus_tag="JZ382_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAY2428074.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="JZ382_001897"
     tRNA            4348..4428
                     /locus_tag="JZ382_001897"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="JZ382_001898"
     tRNA            4435..4506
                     /locus_tag="JZ382_001898"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="JZ382_001899"
     CDS             5021..5728
                     /locus_tag="JZ382_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2428075.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="JZ382_001900"
     CDS             complement(5788..6432)
                     /locus_tag="JZ382_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAY2428076.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="JZ382_001901"
     CDS             6559..6846
                     /locus_tag="JZ382_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428077.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="JZ382_001902"
     CDS             6954..7274
                     /locus_tag="JZ382_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2428078.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="JZ382_001903"
     CDS             7355..8668
                     /locus_tag="JZ382_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY2428079.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="JZ382_001904"
     CDS             8665..9384
                     /locus_tag="JZ382_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAY2428080.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="JZ382_001905"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="JZ382_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY2428081.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3363 a         1669 c         2152 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa
//
LOCUS       DADJYG010000096         9941 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.099, whole genome shotgun sequence.
ACCESSION   DADJYG010000096 DADJYG010000000
VERSION     DADJYG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.099"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            124..3351
                     /gene="fss3"
                     /locus_tag="JZ382_001906"
     CDS             124..3351
                     /gene="fss3"
                     /locus_tag="JZ382_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAY2428082.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3464..3778
                     /locus_tag="JZ382_001907"
     CDS             3464..3778
                     /locus_tag="JZ382_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428083.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3791..4165
                     /locus_tag="JZ382_001908"
     CDS             3791..4165
                     /locus_tag="JZ382_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428084.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4175..4510
                     /locus_tag="JZ382_001909"
     CDS             4175..4510
                     /locus_tag="JZ382_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAY2428085.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4593..5933
                     /locus_tag="JZ382_001910"
     CDS             4593..5933
                     /locus_tag="JZ382_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAY2428086.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6011..6685
                     /locus_tag="JZ382_001911"
     CDS             6011..6685
                     /locus_tag="JZ382_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2428087.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6918..7352
                     /gene="dcm"
                     /locus_tag="JZ382_001912"
     CDS             6918..7352
                     /gene="dcm"
                     /locus_tag="JZ382_001912"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAY2428088.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            7353..8060
                     /locus_tag="JZ382_001913"
     CDS             7353..8060
                     /locus_tag="JZ382_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAY2428089.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            8050..8340
                     /locus_tag="JZ382_001914"
     CDS             8050..8340
                     /locus_tag="JZ382_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428090.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8599..9783
                     /locus_tag="JZ382_001915"
     CDS             8599..9783
                     /locus_tag="JZ382_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAY2428091.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9798..9917
                     /locus_tag="JZ382_001916"
     CDS             9798..9917
                     /locus_tag="JZ382_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAY2428092.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         3291 a         1679 c         2247 g         2724 t
ORIGIN      
        1 taggctcatt cattctcaaa tccattctat tcagtcaata aaaattttaa aaagacttta
       61 taaacaaaga tgttcaagcg agcatcttat ttttttgtgc ttaatttgaa aggagaacat
      121 ttcatgtata aaaaacaaaa aaagaaatta tgtgtattaa ctattttgag tttggctgtt
      181 caagcaattg ttggagcaat tgctcctacg attacttttg ctgatgaaat tacccatcca
      241 caaacggtaa ccgtagaact agatttagca catcaatatg ctgttgaagg aacttttagt
      301 gatggtcgtc caatgtcaga agtcactgtt ccgcattatg cagtgtacaa cggctttaaa
      361 caagatattt tctgtattga accaggagtc ccgatttaca atgagttcac tcctggctat
      421 gagaaaaacc cgttaccaga tatgtccgat aaagccaaat tagtttccgt tttatggaaa
      481 aaagtaggta cagatgcaga tactcatatt gtggcacaga agatgatctg gcaagaagtt
      541 aatgggtata cccttcactc aataaaacga tcagatggta gtgccgtaaa tatcgcagcc
      601 attgaggcaa aaataaatca agccattgac gattatcaga agaaaccaac cttccataat
      661 agtacggcta aaacagtatt agggcaatcg actactgtga cggatacgaa taatctgaat
      721 ttgtcagagt tcgatgaggt tgtggaaaat gccgcaaaca ttgattatcg tgtgaatggt
      781 aatcagttga taatcacccc aaatgccaat tcaaaagaaa gtggtgtgct gacgcttaag
      841 aaatctgctg gtaccggaac acctgtcgcc tataaaaagg ctggccaaca gactttaatg
      901 gccggggcga ttgataaacc taatacctac acaatcaaaa ttgatgtgga aacggagggt
      961 tctttaaaga tcaaaaaagt cgataaagaa tcgggtgatg ttgtaccagg aacggttttc
     1021 catttagact ttggaaaaac tttacctgca aaagacgtga ctactgacaa agaaggcatt
     1081 gctacattgg atgagattcc tcatggtaca aaagtaacca ttaccgaaaa atcagtaccg
     1141 gcgccttata cgattgatac cacccccatg actaccacca ttaaagcagg cgaaaccatt
     1201 tacgtgactt caaaaaacgc acgggaaaaa gggcaaatca ttctggataa aagcggagtt
     1261 gaaacaggga gtgatctttg gaatgacaat tattccttag ctggtaatac gtttgccatt
     1321 cgcaaagaca gtccaaccgg tgaaatcgtt caagaaatga ccacggatga aaacggtcat
     1381 gcggaaacac caaaagagat tgctaatgcg ttggaattgg gaacctatta tgtgaccgaa
     1441 actaaagcta gtcatgggtt cgtgaatacg ttcaaacctg taaaagtcga attgaagtat
     1501 gccaatcaaa ccgtagcccc tgttaccagt aacgtaaaag ggcaaaatca agaagtcact
     1561 ggcgaaacca ctttgacgaa agaagacaaa gatactgggg ataaagcaca aggtaaggca
     1621 gtctttgagg gtgctgaata caccttgttt actgctaagg atggaaaagc tgttaaatgg
     1681 agtgaggctt ttaaaccaga gttagcgaag ggaacgaaag gttctgatga aacggtcact
     1741 ttgactttag acgaaaagaa ccaagttgcc gttaaacatt tagctatcaa cgagtattat
     1801 tggcaagaaa caaaagcgcc cgaaggatat agccttgatg aaacaaagta tcctgtatcc
     1861 atcaaaaagg tggatgatag cgaaaaaaat gccgtgatta cccgaaatgt tacggcaaaa
     1921 gaacaagtga ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgcgggcact
     1981 accgaaactg gcttcaatga tctgaccttt aaagtgtcgc cattggaagg gaccaatgaa
     2041 atcacaggtg ccgaagatga agcgaccaca gcttataacg agcaattagg gtttgatggt
     2101 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaagcccca
     2161 gaaggttttc aaaagattac gccattagaa atccgttcta cttttaagga aaacaaagaa
     2221 gactttgtga agagtgagta tgtctttacc attaccgaac aagaccaaaa acaaccaatt
     2281 aaaacggtga ccgttcctta cgagaagctg acgaacaaag cattttctgt tagtttgaac
     2341 cgtttaatgc tctatgattt gcctgaggaa gaagatagtt taacttctct tgcgacttgg
     2401 aaagacggaa acaaagaatt aacttctctg gattctaccg agctagttga taaattgagt
     2461 tacaatctac atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggat
     2521 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtgat tgcagaaaca
     2581 agcgcaacgt tggcgaacaa agagaaaaca ggaacttgga aaattcagca taaattaacc
     2641 gccgaacaag ttttaaataa aactattgtc ttattcaact atgtgtatga aaataaggaa
     2701 gcttttgaag caggcgataa gccagtagcg aaggatgtta gcttgaataa ccaagcccaa
     2761 actgtcagtt gtacagtgga acaccatgtt tctatccaaa caaaggccca cctagaaaac
     2821 ggttctcaga cctttactca tggtgatgtg gtggatatgt ttgatgatgt gtcgattact
     2881 catgatgtac tggatggctc aaaagaagct ttcgaaacaa ttctgtatgc tttattaccg
     2941 gatggtacaa acaaagaaat ttggaaatct ggcaagattg attatgaagt aaatgataaa
     3001 gaattcacca aaactgtgct tgcgaaaaaa gtagataccg gaaaatatcc agaaggaacc
     3061 aagtttactt ttgcggaaat caattacgat aaagatggga atatcaatgg aaaacacaat
     3121 gaagatttga aagaaaaatc tcaaacctta acaccaaaag aagttccaac catactaagt
     3181 acgccgaaac aaccggaaac accaactgtt ccaagtggct ctcaagaatc tagtcccaca
     3241 gtgaagacct tccctcaaac cggggagaaa aattccaatg ttttactgtt cattggtttt
     3301 actttgattt ttgcgacggc gggctattat ttctggaatc gccggaacta aggtgatgag
     3361 atgatacaag taaatcctaa gaaccaacgt gcgcgaccgc ctcctaaaaa agtagtcgcg
     3421 ctatttttgt atccaaaact aacaatgaaa agaggaaaag aagatggaat tgaaatttgt
     3481 cgtgccagat atggctgaaa cctttggaaa gttaagttat gccggtgaag gagaaattat
     3541 aacagaagga tatggacgga atactacggt gattggtcgc agttatcatt tgtattccag
     3601 taagcaacgg gccgatgata ttgaggtggt agtagctgcg gaagccggtg aaaaggattt
     3661 tgatcaagac caaccgctaa aagccgtgaa tccccatttg gttgccaaag gctatgagat
     3721 tgagaatcgt gggtttactg attacgtgtt gtatgttgat gatttagtga aagcatagga
     3781 ggaaaagaag atgagattaa cagaaggtat tgtcgtagat tcaggtttaa catttgggaa
     3841 gttgcgtttt tccgcattac gccgagaagt acggaagcaa aatgaggatg gaacaattgg
     3901 taatgaggta aaagaacgaa cctataattt aaaatcctct gcacaagggc gaatgattca
     3961 agttagtatc ccagctaatg tacccttacg cgagtttgct tatgatgcag aggtagagtt
     4021 ggtcaatcca atcgtggata ccgttgccaa ttatgttttt cgagaaggaa ctaccgttaa
     4081 ttggttcatt aaggcggatg atttagtctt gaagcggcag ccgaatcaag gaaatcctac
     4141 gaaccaaaat gaaagaaaga aataggtgaa gattatggaa attaatgttg aacatatttt
     4201 agattgtctc gatcaatatg gaaaaggcga gctgactgag gaacaactcg ttaaagcatt
     4261 aacctctgat gaaaaaatgt ttctgattat acatcaaggt ttacttggag aaaatagcga
     4321 cagtgaagaa gactctgatg tactagactg gttaggagaa gaagagtctt tctttatagt
     4381 tgtcgaagta aatgaaaact tatgtcggca ggcagaatcc attttggaag aaattggtgt
     4441 ggagatgccg gatgctattg aaggcttttt aaatcaattg gttgaaacga aacaactccc
     4501 ctcctcgtag cagtaaatga ctagcataac tttttgaaat tgcctaatga aatcctattg
     4561 ggctttttcg attggaggtg gaaaccatca aaatgtacaa aggacatcgc atacgcgcag
     4621 gagatcagca cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt
     4681 ggatgagcgt cttttatttt caagagttta gacaatttga tatttctaag ctatcacttt
     4741 ctactattga aatcgtttgg tctataaaag atttagtatg tctgcttgga agtctcgctt
     4801 tttcaggagc tatgatattg ctttatattc acttttttct tgatcattgg cgaagcttgt
     4861 ggcatcgaca aaagctagct cggatgattc tagagaacca ttggtatgaa gtgaaacaaa
     4921 ctcaaagtga aggctttttc aaagatttga atagtagccg aaccagagag actatcagtt
     4981 acttccccaa aatctgttat cgaatgaaag atggattact gtctattcgc gttcaaattt
     5041 cacttgggaa ataccaagat cagcttttaa aattggaaaa gaagttagaa agcggactgt
     5101 attgtgagtt agtagaaaag gaactcaaag actcctatgt ggagtatact ttactgtatg
     5161 atatgatcgc caatcgaatt gggatagacg aagtagtggc agaaaatggg actctgcgat
     5221 tgatgaaaaa tcaagtatgg gcttatgatt ctttacccca tatgttaatc gctggtggca
     5281 cgggtggagg gaagacctat ttcctcctca ccattatcga agcactattg aagtcagatg
     5341 cagaattgtt tatcctcgat cccaaaaatg cagatttagc tgatttaggt acggtgatgc
     5401 ctcatgttta ttcacaaaag gaagaaattt ctgcttgtgt ggaagatttc tatgaacgta
     5461 tgatggctcg tagcaaggca atgaaagaaa tgtctaacta caaaacagga gagaattatg
     5521 cctatcttgg acttccaccc aactttttaa tctttgatga atacgtggct tatatggaga
     5581 tgttaacgac gaaagaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttgg
     5641 gtcgtcaatc tggtttcttt ctgattttgg cttgccaaag gccggatgcg aaatatttag
     5701 gagacggaat ccgcgatcaa ttcaactttc gcgtggcttt gggtcgtatg agtgaactcg
     5761 gatattccat gatgtttggg gaggtcgaaa aaaacttctt tatgaagcac atcaaaggtc
     5821 gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa
     5881 aagggtatga tttcttagat tcaattagta aattagaaaa gatgaatgat tgagtgatct
     5941 tcaatactga ctttatactt caagtaacgt ataatacttt tatatagtag gaagaaaggg
     6001 gataattaat atggaaagta atgattctta tttagagtat gcaaaagcaa ataaggataa
     6061 gtttatttct tatattatca aagggaaatc ccccgaagtt ggacgagacg cagtttttat
     6121 ggcagggagt cctggggctg gaaaatcaga ggttgccatt ggattagctg agaattatga
     6181 taaccatgtg attattgatg cggattactt tcgcacccag tttcctgatt ataatggaaa
     6241 aaactctagt ctctttcaaa aagcatcttc ttggttggta gagcaggcat ttagatatgt
     6301 cttagatcat ggctattctt ttattctgga tgcaactttt gcaatattaa gtgctgaaaa
     6361 aaatatctcg cgggcgttga aaaaacattt tcgagtgacg attttctatg tttatcaaga
     6421 ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt gcagagggac gtcatgtgcc
     6481 gaaaaaggca tttattaacg cttttttcaa atcaagagag aacattgcaa aagtaaagag
     6541 taaacatcca gatgtattgc ttcatatcat aataaaagat tatacgaata atatttccga
     6601 agttcattat gctacggata atctagaatt atttttacca ctagggtata gtaggaaaga
     6661 tttggaggtt caattacatg actgagaaag aacaagtaac gaaaattgta aagaaataca
     6721 ataagagcat tgcggattta tcggaaaatg ctactgcaaa agagttcaaa acggtgatta
     6781 aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt agttggatta aataaaaaat
     6841 agtaggacaa attaataatg tatgagccaa gagcaaaatg ttcttggctt ttttctttgc
     6901 aatgaatgag gtaatgtatg agatttttag atttattcgc cggtattggt ggctttcgtt
     6961 taggtatgga acaggcgagc catcattgta tcggtttttg tgaaattgat aaatttgcta
     7021 gacgtagtta taaagcaatt catgatacaa gtaaggaggt ggaaatgcat gacatcacaa
     7081 gtgtatcaga tgagtttatt caatccctcg gaccagtgga catcctttgc ggcggatttc
     7141 cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt ttccgatact cgaggtactt
     7201 tattctttga aatcgctcgg ttcaccactc ttctccgacc taagctttta ttccttgaga
     7261 acgtccgagg attgctcaat cacgaaggag gggctacgtt cgagacgatc ctccgaacgc
     7321 tggatggatt ggggtatgac gtggagtggt aggtgcttaa ctcaaaggcc tacattcccc
     7381 aaagccggga gcggatattc cttatcggac attctcgaga cgcatgtacc gaacaagtat
     7441 ttcctatcat tggaccgtct tcaacatctg atcaaaacat cagaaatttg gtaaatatca
     7501 atccttccaa tcgaggaatg ggtggacaag tctatggttc agatggcgta gcgccaacct
     7561 taacaggtga tgaaggaatc aaaatcgcac tgccagcaag ggatggaatc tctgtagcag
     7621 gactattacc gggaaagttt gaacaaggta atcgagttta tgaagttact ggaaccgccc
     7681 ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat tcgaaaaaat gaaacagaat
     7741 atcaagaggt gaagccagaa gacagtgtga atctggcttt tcctaattcc acaagtcgcc
     7801 gaggtcgtct gggaaaacag agtgttcata ccttactaac aggagaccaa caagcgatcg
     7861 tcacggatca gtatcaaatt cggaagctaa cacctagaga gtgttggcga cttcagggct
     7921 ttcccgattg ggcttttgat cgagattctc aagtcaattc agatagtcag ttgtataagc
     7981 aagccggaaa ctctgtaaca gttccggtca tttttgatat tgccagaaga ttaaaagagt
     8041 tggatgtaca tggtttatga tgagttgatt ggagaaattt attgggtgat tggaaagatt
     8101 cagtcagacc cagaattaga agaacaattg cgacggttaa aatttgatat tcgcaagaat
     8161 ggtgtcaaag tcgctggcga tccatattta atgaatgagg agacggatgc tcgaattgaa
     8221 ataaatcaag taattgcaga atttgagcgg attgcggacc aagcaaaaga accagatatt
     8281 cgtcaatact tgttagaaat gaaagcagag ttggaagtaa atggtattac agatgagtaa
     8341 cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga ggattcgcga agcagaaatc
     8401 ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga ccatgatgtc tcacccccgt
     8461 atctaacagg ggggtacaaa aacacaaaaa ataagtaagc aaccattaag aatgcttgtt
     8521 gtatcaaaga tagtaagtaa tttgtatagg tgttaactca tttgaaaaag ttaacacctt
     8581 gaatttggag gtgagaactt ggcacaacgg aatttagatt atcggctatt aaaagaccga
     8641 cgcaacgaat acggcgtttc acaaaataaa ttggctacgg cttgtggact tagtcggccg
     8701 tacttaaatc agatagaaaa tggtggcgta accgcatcta taaaaaccat gaggaaaatt
     8761 tttgaccaac tggaaagctt taatcccgat ttgcctttga cgttactgtt tgactatgta
     8821 aggattcgct ttcccacaac ggatgcacga aaaattattc aagaaattct ccatttgaaa
     8881 tttgagtata tgctccatga agattatgcc ttttactcct atcaagaaca gtatgtcatg
     8941 ggtgatattg tggtgatgtt gtctcatgaa gaagataaag gtgtgctttt agaattaaag
     9001 gggcgaggtt gccgacaatt tgaaactttt ttactcgctc aaaagcgtag ctggtacgac
     9061 tttttcgagg attgtctaaa ggccggtggc gtgatgaaac gcttggactt ggcaatcaat
     9121 gatcgagtgg ggctattgga tatacctgag ctaacgaaaa aatgtcagaa ggaagaatgt
     9181 atctccttgt ttcgtacctt taaaagttat cgttccgggg aacttttaaa ggctgatgaa
     9241 aaggacggca tgggcaatac cttatatatc ggcagtctca agagtgaggt ttatttctgt
     9301 ttgtatgaaa aagactatga acaatatatc aagttaggga ttccattaga ccaaactgag
     9361 acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg cttttcatgc gattcaagat
     9421 ttgttaaaag gtcgtagcat tgagagcacc acgttttcca ttatcaatcg ttacttgcga
     9481 tttgcggata aagtagaagg taaaaggcga accaactggc ccttgaatga acaatggggg
     9541 cgttttattg gacggaatcg gaaagagatt caattaactt ccgagccaaa accgtatacc
     9601 attgaacgaa ccttgaattg gctaggccga caagtcgccc ctacgtggaa aatggctaag
     9661 gagttggatc gattgaatcg gacaacctat atccaggata tggtacagaa tgcacgctta
     9721 tcagaccgac ataaaaagat tttggagcaa caaagtatgg caattgaaaa tttgattata
     9781 tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt tttggtattt ggtggcagta
     9841 tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc agcagcggat cgagcaatgg
     9901 aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt t
//
LOCUS       DADJYG010000097         9624 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.100, whole genome shotgun sequence.
ACCESSION   DADJYG010000097 DADJYG010000000
VERSION     DADJYG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.100"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1153)
                     /locus_tag="JZ382_001917"
     CDS             complement(<1..1153)
                     /locus_tag="JZ382_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAY2428093.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEG"
     gene            complement(2025..3656)
                     /locus_tag="JZ382_001918"
     CDS             complement(2025..3656)
                     /locus_tag="JZ382_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAY2428094.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(3670..5343)
                     /locus_tag="JZ382_001919"
     CDS             complement(3670..5343)
                     /locus_tag="JZ382_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428095.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(5356..5673)
                     /locus_tag="JZ382_001920"
     CDS             complement(5356..5673)
                     /locus_tag="JZ382_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAY2428096.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(5698..7098)
                     /gene="lacG"
                     /locus_tag="JZ382_001921"
     CDS             complement(5698..7098)
                     /gene="lacG"
                     /locus_tag="JZ382_001921"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAY2428097.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            7335..8120
                     /locus_tag="JZ382_001922"
     CDS             7335..8120
                     /locus_tag="JZ382_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY2428098.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8123..8815
                     /locus_tag="JZ382_001923"
     CDS             8123..8815
                     /locus_tag="JZ382_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAY2428099.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(9089..9460)
                     /locus_tag="JZ382_001924"
     CDS             complement(9089..9460)
                     /locus_tag="JZ382_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAY2428100.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2900 a         2110 c         1689 g         2925 t
ORIGIN      
        1 cgccttcggc ctgttggaaa cctttatgac tgcgacctag gaatttttgg ttgtaagtat
       61 caaaagacga gactaagaaa cgttccatcg actcttcatt ttggaactgt tccttgcggt
      121 ggctgtattt cttgatttgt ttattaaagg attcaatcaa gttcgtagaa taaatactct
      181 tgcgtatggc caaggggaaa tcatagaaag tgagcaagtg atcgttcgaa agaatcgatt
      241 caaccacttt tggatagctg gttttccatt tttccgcaaa agtaccacgt gcttccaacg
      301 ccacctcttt agatgaggct tgatacacca ttttaaaatc atcacagact tccttacgat
      361 catcgacacg cactttatga ctgatattac gggaaacgtg tacacaacaa tgttgaaaac
      421 gagctttggg atagaaccga ctaatcgctc ctaccatccc ctttaagcca tccgtgatga
      481 acaggaggac atttttcaaa ccgcgctctt gaaggtccaa taaaatttcc tcccaaatcg
      541 tgatggattc agtcggtgca atcgcatagc tcaatacttc ctttgatccg tccgggcgaa
      601 tgccaactgc gatatgaata gcttccttgg cgacggtttt ccgctttaac ggaatatacg
      661 ttgcgtccat ataaatagca gcataacggt catgaagctc ccgcccatta aacgccgtta
      721 cctcttctgt aaatgatttt gttatattgg acatggtttg gggcgtgtag taatgcccat
      781 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
      841 tgacggtctc ctctaacgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
      901 actcaccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
      961 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcatactttt
     1021 cgtaatctag gaaagccgtt aactccgttt tgagaagcgt attgactgct ttctccaaat
     1081 ggacacggaa taattcattc aaatcgcctt tgttcgctag agtctttaga atttctgtag
     1141 taaaatcgtt catagggaag tcctctcttc tgtgaatgtg ttgtggtaac tctattctac
     1201 agaagggact tccttttttc tatttacaca aaatatttta cactctccat tataacaatt
     1261 gggttaaaaa gaaaatagat agtattctac ctcatattta tttgaaagta atatataaaa
     1321 gacgttcaaa ttcgaacgcc tctcgttaac caaatattgg attcagaatc tagttttcat
     1381 atataaacag agtatgatta ggtgcaaaaa ttgcactttt attgcacttt tatttttatt
     1441 tatagtgttt tactgttctg tatattgttt taactccctt gatagtatcg cattctgttt
     1501 tatacacttt acgttgaaaa cagagtggga ataaagagcc aacataatat ggagggggaa
     1561 tccctataaa tcaacattct tcaatagcta aaatgcttga ttttcattaa aaataagatg
     1621 gttccctacc aaaagagact ataatataag ttaatttgtt tcattcttat cataaaaaga
     1681 agttctcgaa aaacgaactg ttttccgaga acttttttct atccatctaa gtgctcaaat
     1741 catattcacg acggaattct aaggttaccc tttcttaaac aaactttatg ttatctgcac
     1801 aactttttgc aattttttca ttgcttctta cgaaatcgtt gaactctctt tagcataata
     1861 caaagtgtag atgaagtctg gtgctttact cgttttggta aaaatgactg gaaatcgttt
     1921 ctggttattt ttggcaaagg cgaggtgcag gttcgattcc tgccttctac gctaacgatg
     1981 tggctgaagg gaacctccga tcttgaatcg aaacgatccg cagtctattg tttcaccaat
     2041 gaaacaaaat caacgttcgt ccaagcattt cctgaagcct tgctataaac cccaatttca
     2101 caaacacctg ttgtaattgg aatgtctttg attgtaattt ttgtccattc acttgtttta
     2161 acaaccgcag tatttagttc cgctccacca taatttttga tatataactg attagtatca
     2221 aactctcccc cagacattac ataggcactc aatgtgtaag ttcctttgct tatgccacta
     2281 acattttgat aagctgatgc ctcgtatgct tgatcctgcc aaaaagttag tttgtagtcc
     2341 ccaaaaaaag tgcccgcttc cgttttgatc gtacccttag cggttccttg ttgaaaccag
     2401 gtttgccaat tggaaggact ggtcgttggt ccatcgcttt cgaaacttgc atttttaact
     2461 agatttattt gcgcccctgc ttgtttaaac gcacgcaagc ttggcaaggc gtttccagca
     2521 aaatcaaaca tcgcttggtt gtcccattcg tttcccatag tggattgaac actgagataa
     2581 ttcatacctg cttgtgttgc ccaactcaca tttccgttgt accaaagagg ttcccaatag
     2641 aagaagcccg ccccgcgatt attcggtact gcctgaatcc gatcggcaag atccagcaaa
     2701 tactctgctt gtcctgtcgg tgttggttca tatccccccg cagctgcttc ggcttcacca
     2761 aaagcatttg gcatagtatc ggcattcgct gttgtatgcc cataagctgt ttcaactacg
     2821 atcacttctt taccataccg ttggctgatc gcattcatat tggtttgaag ttcattcatt
     2881 gaaccgtgcc aatacggata atacgacact ccaattaaat caaatgacac acctcgactc
     2941 gtgatttcat caaaccacca agtaaatgct cctgtatcgc caccatcagc taagtggagc
     3001 attactggag ttgttttagg ttgagtgtct cgaacagctt ggataccaga cttcaaaaaa
     3061 gcagccaatc gatcaaattc tcccccgcct tctccccaag acttgccata aggccagagc
     3121 attccgctat tcagttcatt gccgatctga accatatcag gataaacgtc cagctgcttc
     3181 atttccgaca gaacgttctt tgtatagtcg tacaatgccg tattcatttc ttcaaaggtc
     3241 agaccttgcc atgctttagg aagattctgt ttcccaggat caacccaaaa atcactgtag
     3301 tgaaaatcca gcagcacctt aagtcccaga ttttttgctc gttttgcaag gtcaatcgtc
     3361 gtttccagat catttgtccc tgctccataa ctttctccat tactattata aggatcctgc
     3421 cacaaacgca gtcgaacata gttgacacca ttatttttta aaatctctaa ggcatctttt
     3481 gaaatatcgt cttcataata ttttgcacca gatttttcca tatctgacag gatcgaaata
     3541 tctgctccgc gaataaattc gctggcagat gcttcaacag tttttgaaac agcccccaaa
     3601 gcccctaaca cagctgcaaa taaaacagct ttacgcaacc aaattcccat tttcattatg
     3661 gctcctcctt tattgaaatt ggttcagaat aaactgaaca gctttttcag gatctctcgt
     3721 taatccgata tattctgctc cttttgtcgc aaccagcttg attcctaagc gatcggtgtc
     3781 ttctttgata tcatcatagt aagaattgac ttgcggtgct aagaccacca tatcaaaatc
     3841 ctgcagcatt tcgtaatgag aaccataagc acccgccatt gcactgatcg gtgctttcga
     3901 ttcagatgca ccttttttta aagcattagc taacatcgca cttgtccctg cgccagcaca
     3961 aagtaccaac acattgattt ctttatttgc atcgctgata acttgactcg ctgccacagg
     4021 tacttcctca cgtacatcgg cagaagcacc ttcctcggtt gcgatcacct ctgctttttg
     4081 tgcttctttc tctactaaga cagcatcgta tgctttgcaa aatggtagat agatcaggaa
     4141 gtcaacgacg attagaaccg ctgctagaac aaaagctaag atacccatgc ccgttcctag
     4201 aaccaaaccg atcggtgctg gagttgccca aggcaaaacg tacataaagc tgttcatgcc
     4261 caatacatcg acaaaaaact tgaacaggca aacgtttaca acaggtgtta acaaaaatgg
     4321 aataaaaaaa taagggttca aaatcatggg tgccgcaaat agcaatggtt cgttgacagc
     4381 gaaacttact ggaataaagg aagctttccc aactgccttc aactgcttag acttagcaaa
     4441 gagtaggaag ataaaaggaa caaccaatgt tgccccagtt cctcccatag ttcctacaaa
     4501 attccctaaa cccaccgtca gtactttaga agcttgttca ccggaattga acaattgcag
     4561 attggtttcc acattggcat agataattgc cgcaattgcc ggctccacga tcgacggtcc
     4621 atgaacaccg acaaaccaaa acagcgccat cgctccccag ataatagcga ttcctaagta
     4681 cccgtcagct gctgtaaata gcggctgcag caccgtaatg attgcttctg caaatgagat
     4741 ttggaagatt tgtcgaatgc ctaaatcaat taaaacggcc gcaaatactg aaaaagagaa
     4801 aggaaaaata tctcggaagg tttgcgaaat cgttccagga acttctttag gcattttaat
     4861 ggtaatatct ttcagcacac aaaacttata taaattaact gttaaaaatg cagcgacaaa
     4921 ggaagccagt agtccctttg tccccatata ttccgttgaa aatccacctt caatcggaga
     4981 gacgcttaac aacaggaaac ttaccacaga ggccagcatc acagaggtgg tgttgattac
     5041 tttgtttttc ggtaagcgac gattcatcga atcagccaag cagcgagcag tggttgctga
     5101 aacgagtaac cctacaacac ccatcgaata attatagact ttccatagcc atgtggagac
     5161 agattcaggc cattggtaac caaaaatctc aggaaccgca gcaatcaaaa tgaaaatgct
     5221 tgaaaataaa atagctggca tcgctgttaa aaatccgtca cgaacggctc ctaaataact
     5281 attacgtgag attttttcaa aaaatggttt ccctttttcg atttgtttaa tgattccatc
     5341 catagcgttc ttctcctatc cttgattctg tctgtacagt acaataaagt cttgaatcag
     5401 atctctcagc aataaagtgg tcattaaatg atcctgccca tggataaaaa tgaatcccat
     5461 ctccatctct ttcccagatg cttcttccgc taaaattttt gtttgcgaat tatgtgcaag
     5521 ggttaaattt tcgtcggctt ctttcaacgc cgaatcaaca tgttcaaagt ttccttgtct
     5581 tacttctttc aataatttca ataatgtact gcgagcgtcg cctgaatatg caacgatctc
     5641 aaaaccaagc atttgtaatt cctctttatt catcttttct tcctccatta tcacttatta
     5701 aataatcgtt ttggtttcac tgaccaactt gtaccagtaa gctgattctt ttgggtaacg
     5761 cttctgggtt tcaaaatcaa cataaaacaa gccatacctt ttgttatacc catttgtcca
     5821 tgagaaaaga tccataagcg accacaaaaa ataccccttc acatttaccc cggctgttat
     5881 cgccttgctt aaggattcca aatagactct caaatagtcg attctcggct gatccataat
     5941 aatgccatct tcaaactgat ctttataccc catcccgttc tcagtgatat agattttgtt
     6001 gtaatgtggg taatcgcttt taattcgcag caacaagtca tatagacctt ctggataaat
     6061 cagccagtcc caatcagttc gaggaattcc ttctttataa atacgttctc cgattccctt
     6121 cactttataa acagaggtcc ctttttcacc cgtaccgtta tgatggattg cattttctcc
     6181 atcgtaagct ttgacaaaat ggcattggta gtgattgatc cctaagtagt cattacgcgt
     6241 ggatgctttt ttcaattcaa cgaaatcttc ttcaggaaaa tgatagctgg cctgatttgc
     6301 ttcacatatc tcatcaagag cagtcaaggt ttcagttgaa taatagccta aataggtagc
     6361 atctaataag aaacggatcg acaatgcatc gtctaaaaag gcagcatgct tatcttccgg
     6421 cgcgtctgtt gccgcatatt tcgtctctaa tgagtgaact acaccaattt cacccggaag
     6481 ttcgttttct ttgaaatagt tcactacacg ggcatgagca accatcatat tatgaagaca
     6541 ggcgacgatc tttgtaaaat catatttgat tcctggtggg aaaacaccta acaaatattg
     6601 attggttgca actggataga tttcattaaa ggtactccac accttgactt ctttgaattc
     6661 atgaaaacaa aaaatggcat aggaaacaaa tgcttctatt gtttctcgat tcaaaaaatc
     6721 tccatggtca aacaaacgtt taggggtatc aaaatgatgc aacgtcacaa acggcgttac
     6781 gtttcttttc ttacattctt cgaacacacg atgataaaaa gcgattcctt ctggatttgg
     6841 tttcccagct ccatcaggaa aaatgcggct ccaagcaatg gataatctta gaccatttac
     6901 accaaaacgt tcacacaatt caatgtcctt tgcatattga tgataaaaat cacttgccgg
     6961 atcgggactg aatcgccctt gttcctctaa aaagtcatcc caagcaacag cgcctttgcc
     7021 accttcctta gtggctcctt caacctgata ggctgctgtg gctccgccaa aaataaaatc
     7081 ttctggtaat ttaagcatgt acatctctcc tcaaatctat aatcagtttt cgctctttct
     7141 aactgattgc attgtaaacg catccaaaac atttgtcaac aaaaacaaac acaaataatc
     7201 ataaaaacca gaaaacagca ataaaatcta ttgtaacaaa cataaaaacc accctaaatg
     7261 tttacttttg ttcgaattag attgactgtt gtccgtttat atgatagatt atccttaaat
     7321 tagaggtgaa gacaatgttg aaaaaagaaa gattgcttac tatcgtagaa atggtcaata
     7381 aaaaaggaat ccttaccgtt aatgaaatca tcaatcaatt agatgtttct gatatgaccg
     7441 ttcgcagaga tttagatgaa ttagaaaaat cgggcaagct gctgcgtgtc catggagggg
     7501 ctcaaagtct ttcctacact ttagatcaag aactttctca cactgaaaaa gccactctgc
     7561 agattcaaga aaaaaaagaa attgctgcca cagcagcctc cttgattcac gagggagaaa
     7621 cgattttctt aggaccaggt acaacaatcg aactattggc gacgcattta ctgaacaaga
     7681 aaattcggat cattacaaat aattatccag tttttgatat tcttgctcaa gcagatggac
     7741 cagaaattct actgattggc ggtgatttcc ggaaaaatac cggcgcattt gtcggaccga
     7801 tcaccaacga caatttaaga agactaaagt ttacaaaagc atttatcagt tcaaatggcg
     7861 ttcacaacga agcgatctct acttacagta ccgaagaagg agaagcacag cagatcgctc
     7921 taaataattc ccgtaagaaa tatctactcg ttgataataa aaaattcaat cgagatgatt
     7981 tttatgtgtt ttataatttg catgatttcg atctgctcat tacggatcgc cagatctcgc
     8041 gggaggttca agagcactat gcacaatatg tcgatctgca aattgctgat tcaaccgaaa
     8101 ataccactat aggagcgtaa ctatgaaagg gattatattt gatttcaacg gaacaatgtt
     8161 tttggactct catctgcatg aagctgcttg gctgcatatg atacatgatc atacaaaaga
     8221 tggtttatca gacgaggata ttttaaaaaa tattcatggt cggacgaata cggagatttt
     8281 gaaacatttc atttcagagc atttgaccgc ctctgagatc gccgtattgt cagaagaaaa
     8341 agaaagctat tatcgtatgc tttgcttgca aaatgaaaac gagcttcagt tgacaaaagg
     8401 gcttgaagcg actcttgacc agctgactga ccaacagatt ccattaacga ttgccacggc
     8461 tactgtcaaa gagaatgttg ctttttattt tgatattttc gatttagccc gctggtttga
     8521 ctgggacaag attgtctatg acgacggctc tttccctggg aaaccgcaac cggatatttt
     8581 tcttaaagcg gcagacaaac tgagcttagc tccggctgat tgtcttgtta tcgaagatgc
     8641 ttactcagga ttacttgctg ccaaacgtgc ggggatcgga acaattattg caattgatcc
     8701 attcggtaaa aacaaaacag tatttgaaca ggcacagcta aataaagatg gaaccatcaa
     8761 agattttact ggtttttgga cagagcacct gtctgccaca tccgttaatt cttaaacgat
     8821 tcaactaact accaaagaaa agcaactgaa atgcacacct ccttttataa aaaactgcca
     8881 gtgaaacgac atgaaagata cactgctata tccttagtga aatatagcag tgtatctttg
     8941 actttttcat tcttttaacc atatgacgcg ttcatctcta tctataatta acattaaaaa
     9001 tagtgttttc acattatttc tcaaaccaat cttaaaaatc atcaggagta cttttttgat
     9061 atacttttat taatgctggg cgtaatggtt aaaagatttc cactagttcc tctccaccaa
     9121 gctgagttga aaaatgctta tctgtaaaca acatagacaa agaggaaata aacgtttccg
     9181 tatctttctc tgtcccaagg atattaatta attttcctac taggtaagta tcattaaact
     9241 taagatctgc cttcacatca aaagttttga taaaagcttt tatcatgcgt acaattttgt
     9301 ctttatcgcc ataattgatt tcaccactca tatcaatata gtcactaatc cctgaagaaa
     9361 taatggcaac tgcttcttct ttcgccttat cagataattc atttaatgac tgggattgat
     9421 tgatgacttc ttgagtttta tctgttacgt aatctctcat tatcctatcg gcatactccg
     9481 caaagtaatc ttgaaaaaat tcttctacag ggtaaccaac ttcgttttcc caaccaggag
     9541 aaatttcatt atcagtactt attaaatcta ttaaaaacca acccgttctc tgagtgttga
     9601 ttctataata tttttaattt ccta
//
LOCUS       DADJYG010000098         9431 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.101, whole genome shotgun sequence.
ACCESSION   DADJYG010000098 DADJYG010000000
VERSION     DADJYG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.101"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            135..2606
                     /locus_tag="JZ382_001925"
     CDS             135..2606
                     /locus_tag="JZ382_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAY2428101.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="JZ382_001926"
     CDS             complement(2709..4445)
                     /locus_tag="JZ382_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAY2428102.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="JZ382_001927"
     CDS             complement(4442..5146)
                     /locus_tag="JZ382_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2428103.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="JZ382_001928"
     CDS             5277..5780
                     /locus_tag="JZ382_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAY2428104.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="JZ382_001929"
     CDS             5740..6078
                     /locus_tag="JZ382_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAY2428105.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="JZ382_001930"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="JZ382_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY2428106.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="JZ382_001931"
     CDS             7629..8207
                     /locus_tag="JZ382_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY2428107.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="JZ382_001932"
     CDS             8211..8732
                     /locus_tag="JZ382_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAY2428108.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="JZ382_001933"
     CDS             complement(8811..9365)
                     /locus_tag="JZ382_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAY2428109.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3056 a         1741 c         1963 g         2671 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa a
//
LOCUS       DADJYG010000099         9288 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.102, whole genome shotgun sequence.
ACCESSION   DADJYG010000099 DADJYG010000000
VERSION     DADJYG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.102"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(19..687)
                     /locus_tag="JZ382_001934"
     CDS             complement(19..687)
                     /locus_tag="JZ382_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2428110.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            821..1108
                     /locus_tag="JZ382_001935"
     CDS             821..1108
                     /locus_tag="JZ382_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428111.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1524..3062
                     /locus_tag="JZ382_001936"
     CDS             1524..3062
                     /locus_tag="JZ382_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAY2428112.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3059..4024
                     /locus_tag="JZ382_001937"
     CDS             3059..4024
                     /locus_tag="JZ382_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428113.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4011..5333
                     /locus_tag="JZ382_001938"
     CDS             4011..5333
                     /locus_tag="JZ382_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY2428114.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5396..5500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5574..6317
                     /locus_tag="JZ382_001939"
     CDS             5574..6317
                     /locus_tag="JZ382_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2428115.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6332..7324
                     /locus_tag="JZ382_001940"
     CDS             6332..7324
                     /locus_tag="JZ382_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2428116.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7336..8073
                     /locus_tag="JZ382_001941"
     CDS             7336..8073
                     /locus_tag="JZ382_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428117.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8063..8785
                     /locus_tag="JZ382_001942"
     CDS             8063..8785
                     /locus_tag="JZ382_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAY2428118.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8869..>9288)
                     /locus_tag="JZ382_001943"
     CDS             complement(8869..>9288)
                     /locus_tag="JZ382_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAY2428119.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3039 a         1603 c         1966 g         2680 t
ORIGIN      
        1 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
       61 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
      121 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
      181 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
      241 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
      301 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
      361 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
      421 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
      481 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
      541 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
      601 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
      661 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
      721 acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
      781 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
      841 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
      901 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
      961 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
     1021 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
     1081 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
     1141 caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt
     1201 tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt
     1261 ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg
     1321 tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg
     1381 ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt
     1441 gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa
     1501 agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag
     1561 gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg
     1621 cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag
     1681 tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac
     1741 ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt
     1801 tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag
     1861 aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca
     1921 tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg
     1981 ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg
     2041 accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta
     2101 tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt
     2161 tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa
     2221 atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt
     2281 ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca
     2341 agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca
     2401 caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga
     2461 caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat
     2521 tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga
     2581 acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg
     2641 gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg
     2701 gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata
     2761 atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc
     2821 tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga
     2881 aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa
     2941 aaataacgga ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc
     3001 cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt
     3061 gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga
     3121 tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag
     3181 tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag caggagacat
     3241 ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg
     3301 atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt
     3361 catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct
     3421 tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg
     3481 aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt
     3541 gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt
     3601 tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga
     3661 agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga
     3721 cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga
     3781 accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa
     3841 tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata
     3901 ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc
     3961 gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat
     4021 atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag
     4081 aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc
     4141 gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac
     4201 aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat
     4261 tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg
     4321 aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg
     4381 aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac
     4441 cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc
     4501 ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca
     4561 atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg
     4621 aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt
     4681 ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag
     4741 agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat
     4801 ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt
     4861 atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc
     4921 caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg
     4981 caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac
     5041 ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg
     5101 atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga
     5161 cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat
     5221 tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct
     5281 atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat
     5341 gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct
     5401 gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct
     5461 gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg
     5521 tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga
     5581 aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca
     5641 tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag
     5701 cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg
     5761 gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt
     5821 ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt
     5881 tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg
     5941 tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa
     6001 aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat
     6061 ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt
     6121 gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca
     6181 tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca
     6241 ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta
     6301 tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct
     6361 actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa
     6421 ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat
     6481 ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga
     6541 accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt
     6601 aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc
     6661 aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat
     6721 ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga
     6781 agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt
     6841 aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt
     6901 tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt
     6961 gaaagaatta gatgaacggt tagattacta tactccggta attattacga atgatcaact
     7021 gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca
     7081 ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa
     7141 tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc
     7201 tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga
     7261 aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa
     7321 ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg
     7381 aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg
     7441 ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact
     7501 tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat
     7561 atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat
     7621 caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt
     7681 gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga
     7741 acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt
     7801 ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa
     7861 aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca
     7921 gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct
     7981 gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat
     8041 cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca
     8101 gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc
     8161 agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg
     8221 tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa
     8281 agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac
     8341 acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt
     8401 actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca
     8461 ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg
     8521 ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc
     8581 agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt
     8641 aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat
     8701 cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga
     8761 acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt
     8821 tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc
     8881 gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg
     8941 ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa
     9001 ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg
     9061 tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc
     9121 aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg
     9181 tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
     9241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DADJYG010000100         9020 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.104, whole genome shotgun sequence.
ACCESSION   DADJYG010000100 DADJYG010000000
VERSION     DADJYG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.104"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..523)
                     /locus_tag="JZ382_001944"
     CDS             complement(<1..523)
                     /locus_tag="JZ382_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAY2428120.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYY"
     gene            complement(541..1440)
                     /locus_tag="JZ382_001945"
     CDS             complement(541..1440)
                     /locus_tag="JZ382_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAY2428121.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDTQLREK
                     KFVKKYQALVSGMLSNLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYSGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(1442..2239)
                     /locus_tag="JZ382_001946"
     CDS             complement(1442..2239)
                     /locus_tag="JZ382_001946"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAY2428122.1"
                     /translation="MKVAIVHNKEAKTIEVAERLNTLLEQAGIQRDDHEPELVISIGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNLEQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKADIRSIYYRIAEERIHFASYRHMHFWHRVKDAFIGED"
     gene            complement(2214..2921)
                     /locus_tag="JZ382_001947"
     CDS             complement(2214..2921)
                     /locus_tag="JZ382_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAY2428123.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRSQ"
     gene            3052..3627
                     /locus_tag="JZ382_001948"
     CDS             3052..3627
                     /locus_tag="JZ382_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAY2428124.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDNDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEEGRLETTDTFVTEEAEAFIFQKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIKEGLLAIDESWGDNLHDFELELEVVDASA
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            3745..4422
                     /locus_tag="JZ382_001949"
     CDS             3745..4422
                     /locus_tag="JZ382_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAY2428125.1"
                     /translation="MIEIYLFVNPLGEYCFKMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIEDVMQQKGISQNNLEKRNHLFSTTYSAALDCKAAQFQGKKKGRHFLIKLQE
                     AVGCNNIPYSHALAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVEGTSSLWRIKELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(4494..6302)
                     /gene="pepF"
                     /locus_tag="JZ382_001950"
     CDS             complement(4494..6302)
                     /gene="pepF"
                     /locus_tag="JZ382_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAY2428126.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDKEFDEKYLELSEKLKKS
                     EKYKGTLNQGASQFLDAIEFVLSVYRQTEIIYVYAHLKNDQDTGNTEYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDERIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSADQESL
                     LAGAGEIFEASSDTFAVLNNADLVFPTIEGENGEKVQLSHGVYGQLLESTDRSVREAA
                     FKGLYSVYEQFRNTFASTLSTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMTIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSSQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKRILSQEPDALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(6482..7480)
                     /locus_tag="JZ382_001951"
     CDS             complement(6482..7480)
                     /locus_tag="JZ382_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAY2428127.1"
                     /translation="MLLAMNEYHECCSAKYAEKEKMYYCRSCRKRVVLKKGKKKCAHF
                     AHRKTENCSVFSEGESEEHLQLKECFMDWLGQSAEPVFLEAYLPRLRQRPDILLANLA
                     LEIQCSRLSHQRFFERTKNYLNNGYQVWWILGHSFLGQRQFSLIEKSCCYYNRKRGVH
                     CWKADLKNQKLYLYHHITETVSGHISFFSSCWTFSGNDLKKIFTNNEIKINQMKKTDK
                     LSEDGKKWLARQLIYKQKNTVSIQEQCYLRHKHLLYLSPWIYQNSRFFFYLREQVFLY
                     RMLYEESLKQQKVPDFSSWFCQVKEYKREWLFPMIEEEMVYRQFFDECTHLSSWKI"
     gene            complement(7578..8231)
                     /locus_tag="JZ382_001952"
     CDS             complement(7578..8231)
                     /locus_tag="JZ382_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAY2428128.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSAVDSEDVSEFLR
                     QQIIGSDPEEELSGSALEEQERQAIFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLSEYGKCLMARNAIERTKFYFV"
     gene            complement(8562..8960)
                     /gene="spxA"
                     /locus_tag="JZ382_001953"
     CDS             complement(8562..8960)
                     /gene="spxA"
                     /locus_tag="JZ382_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAY2428129.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         2640 a         1937 c         1494 g         2949 t
ORIGIN      
        1 tgtaatagat tgtttcagca acatctgctt cattcttcag cacatacatc gcagagcgaa
       61 cggtttgatt tgctacgatc gaaacaaatt cagtagtcat gattgcacca gctgtttcgt
      121 cttcgtaatg gagcaattcc ttgatttcac tggcatcgtc tgcagacatc aaacttaaat
      181 atttagcaat ttgcttttta tctaacgtat taagcaaatc gacagcatta tctgtataca
      241 tctcagaaag catatctgct gcatagctag gacgcatctc atccagatat tctttcatat
      301 gttcgttgtc ttcttcaatc acatcgaaca tatccgctaa ttctttaggc gacagatagg
      361 aatagatcag ctgcctgttc tcttctgtaa gagattggta gaattgcccc tgctcataga
      421 tatgcatctc taaaaaactg tttctgaatg cctgaatatt attttccctt aaatcttgag
      481 tcaactgggc aaatctttcc tctaattctt gattttcatc caaaccaatc tcacctcgcc
      541 ttattcttca aaccgtctca caactgcctc catgtctgcg gctaaaggct gcttgaactc
      601 tagcagctct tctgtaaatg ggtggtaaaa acgcagatca taacaatgaa gggcttgtct
      661 ttttattccc agatccattc gtccgctata catatcatca ccgagcagtg cacagccaat
      721 ggcggaaaaa tgaactctta tttgatgtgt ccttcctgta tgcagctgga tatcaactaa
      781 cgcgatttct gttagtcgct tctctatcca gtactccgtc tgtgcagaac gtccttcatc
      841 agagaccatt cgcttcagta gcgaagaata atctcgggca atcggcaaat cgatatgacc
      901 atgtgccgat aaatttgaaa gcatcccact gactaatgcc tgatattttt ttacaaactt
      961 cttttcacga agttgcgtgt ctaattttgc gtgcgcaaac ccatgcttgg caaaaagcat
     1021 caagccagac gtatcacggt ctagtcgtgt aactacatgg attacttggt tttcgtaccc
     1081 ttgttttttg taataagcct ttacacgatt agccattgtc ccatttggat ggtattgggc
     1141 aggaatggac gaaacgcctg ctggtttgtt tactaccaga acatgttcat cttcatacac
     1201 gatatcgatc ggcgtttcgt ccaaaagtac ggtttcatgt tctccttcag caggaatcac
     1261 aatggttacc ttatcatcct tggctagaga aaacaagaca ttttctactt gatcattgac
     1321 tttgatttga ccgccttgaa attttatttt cgctaacaga ccttttgaga tccctttttc
     1381 ttttaaaaaa tattttacct gctgagaatg atctttctca taatcccatg taaactgcat
     1441 attaatcctc accaataaag gcatctttca cgcgatgcca aaaatgcata tgtcgataag
     1501 aagcaaaatg gatccgttct tctgctattc gataataaat agaacggata tcggcttttg
     1561 cgacattcaa ttgatcaacc gttacaagat aatcttctgt attttccaat tttatttcta
     1621 accactcatc atgggcaatc acgattggag aacccaaagt acgaaacact cgattattca
     1681 gcgaagcaat ctcagccaat tgaaaggcat tgatactagg atggatcact gcaccgccga
     1741 cacttttatt ataggcagtc gaaccagtgg gtgtagaaat cgtcaatcca tctcctctga
     1801 aactttcaaa caattcattt ttaatgtata catcggctac catcgtacga ttggcccgtt
     1861 taatcgttga ttcatttagt gctaaaaaat gctgatcagg cgtttcatcc aaataactga
     1921 tccgaacatc cagcaaggga tagctgacac tttgttccag atttgttcgc aagctgtcta
     1981 ctagttcttc cagctcgtaa tctcgccaat cagtataaaa ccctaaatga cctgtatgca
     2041 cgcctaagaa acgcacatca ttcagacaat gactgtagtg atgaaatgcc gataataatg
     2101 tgccatctcc accaattgag atgaccaact ctggttcatg atcatctctc tggatcccag
     2161 cttgttctaa tagtgtgttc aagcgctcag ccacttctat agtctttgct tctttattgt
     2221 gaacgatcgc tactttcatc ccctgcctcc tctctttcgc tttctttctt caaaagcgtt
     2281 cgatagtaga catcatctga ttttcctctt ccatgggaga acaatcgctg tgcttcttgg
     2341 atttcttcac ggatactgga catctcttca tccagttgat acgcagcttc tgccgcacgc
     2401 tgaagtcgtt cgctcatttc ttctggaaaa acgccttgat atttataatt cagcgaatgc
     2461 tcgatcgttg cccagaaatt catcgcaagg gtccgtattt ggatttccgc caaaattttc
     2521 ttctcaccac tgatcagctg tacaggatac tcgatcacca gatgatacga acggtagccg
     2581 cttgctttct tattagtgat gtaatcccgt tcttgaagaa ctttcatgtc ttttctttta
     2641 cggataatct caacaacttg atggatatct tctacaaact gacacatgat ccttaagccg
     2701 gcaatatcag acatttcttc ttctaagcgt cccattggaa tatgacgtaa ctgtgacttt
     2761 gttacgatac tgtcaactgg tttgactctc ccagtgacaa attcaattgg aacatgacga
     2821 ttttgttcac gaaactgttt acggattccc cgcagtttca ctttcaattc tgatacagtt
     2881 tgttcatatg gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac ctctacttca
     2941 ttaacattca ttgtttattc taccatatat gcaaagtttt ttcgtactct tgagttatgc
     3001 attgaccaaa aaaagaaaat taggcaaaat agacaaaaag gagtcgatca aatgacgcaa
     3061 atgcttgaaa tcgaatataa atctatgctg acaaagcaag agtacgagat attcatcgct
     3121 cactatcaat taacagataa tgatttccgt gtacagacta atatttactt cgataccgtt
     3181 gatgaacagc tgaagaaaca aaattgtggc ctacgaatcc gtttcgttgg tcatcaagct
     3241 gaatatacat taaaaacacc tgctgaggaa ggtcgtctcg aaacgacgga tacctttgta
     3301 acagaagaag cagaagcatt catctttcaa aaacgtttgc ctcgctcagg cgcagttttt
     3361 caaaaacttt ctgatttgaa tatcgatcct gcttcattga tccaaacagg aaagcttaca
     3421 acaaaacggg ctgagttccc cattaaagaa ggacttttag ctatcgatga aagctgggga
     3481 gacaatcttc acgattttga actagaacta gaggtagtgg atgcatctgc gggaaaaatt
     3541 gcttttataa attttttaaa acgtttttct ttgccttatc gtcctgctaa gaacaagatt
     3601 caacgtatgc ttgaggctaa aaattagaaa cctttcattt tcacgaggca cttgagttca
     3661 tttttttact tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata
     3721 gaatctattt tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg
     3781 gaatattgtt tcaaaatgga gcaacagtta ttacaattta ttgaagaaga gtacggaaca
     3841 ccatcaaaag aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattgag
     3901 gatgtcatgc aacaaaaagg aatttctcaa aacaatcttg agaaacgcaa ccacttattc
     3961 tcaacgactt attctgccgc attagactgc aaagcggctc aattccaagg aaagaaaaaa
     4021 ggccgccatt ttttgatcaa actgcaagaa gcagtcggct gcaataacat accatattct
     4081 catgcacttg ctgaatcgat atttgaagag attggcggcg atttgaatat gtttaaagaa
     4141 gatcggcaat ctgactttgt taaagaagta ttcttttccg atcaaaaaat tgctcgagaa
     4201 atgggcatca cgaaacatcc ttccgcagtc gtttacaatt acgcttgcga tcgtgatttt
     4261 ggtgttttag tagaagtaga aggcacaagc agcctttgga gaatcaaaga gctgtgtaaa
     4321 acaacagata actcgtatca agtttttaat ggtgaacatt tacatacaaa tgattctcag
     4381 ttccattcac gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc
     4441 ttacaagaga gccgacgtat ttttttcgtt tctattcgaa aaaatcaaaa atcttataaa
     4501 cgatcaatca attcttctaa ttcgtttagt cgttgttcaa acatcgacat tgcatcttcg
     4561 atatacgcag cctgtgtcat atctactcct gcttttttca tcacttcaac aggataatca
     4621 ctgttaccag cttttagata agccaaatag ttctctagcg catctggttc ctgactaagt
     4681 attcttttcg ccaacgctga agcagcagaa aaaccagtcg agtattggaa gacatagtaa
     4741 ttatagtaaa aatgcgggat ccgtgaccat tcaaatttga tttctgggtc ttcttcgact
     4801 gccggaccat aatattttgc attcaattta ccataactat cacttagata ttcactagtc
     4861 aatggcacac ctttttcatc ttccgtatgc atgaagtgct cgaattcagc aaattgtgtc
     4921 tggcggaaaa ctgttccttt aaatccatcc aagtaatggt tgagcacata agctcggacg
     4981 cgagggtctt tttctgtttc caataaatat tctgttagga tattttcgtt cgttgtcgaa
     5041 gctatttctg ctaaaaagat ggagtagtca ccgtatacat aaggttggct cgatcgtgtg
     5101 aaataactgt ggacactatg tcccatttcg tggacaagcg taaacaattg atccaatgta
     5161 tcatgccaat tcaataaaat atatggattt gtgtcatagc ttcccgaaga ataggcacca
     5221 cttcgttttc ctttattttc gacaacatcg atccaacgtt cagagaatgc tttttctaca
     5281 atagtcatgt actcttcgcc cattggcttc agtgcttcca aagctttctc ttttgcttct
     5341 tcatacgtaa aggtaattgg agcttctcct aagaccggtg tataaagatc gtacatgtgc
     5401 agtttttcca cttctaataa acgtttccgt aattccatat atcgatgcag caaaggcaaa
     5461 tgcttgttta ctacatctac caaagtgtcg tatacacttt caggaatgtg attattgctc
     5521 aaagatgctt ctctggcaga gctgtaatta cgaacttttg ctttaaaatt atgtcctttt
     5581 atatgcgtac ttaaagtaga agcaaacgta tttctaaatt gctcataaac actgtacaac
     5641 cctttaaatg cggcttcgcg aacacttcga tccgtgcttt ctagcaactg accgtacaca
     5701 ccatgagata attggacttt ttcaccattc tctccttcga tcgttgggaa aactaaatct
     5761 gcattattca aaacagcaaa tgtatcactt gaagcctcaa agatttctcc tgctcctgca
     5821 agaagcgatt cctgatcggc agataaaaca tgggcacgat tatctactat ttgctggata
     5881 tagtgacgat agacttctaa cttcggttct tctttgaaat attgccaaat ccgttcatct
     5941 gataactgca atatttctgg ttcaaaccag gaaacagctt cactaacctt tgaaaacaaa
     6001 ctacttgctc ttgcataaag cgcttgatat tctgtattcc cagtatcttg atcgttttta
     6061 aggtgtgcat agacataaat gatttcggtt tggcgataaa cactcaacac aaattcaatc
     6121 gcatcaagaa attgagaagc gccttgatta agtgttcctt tatatttttc agattttttt
     6181 aacttttctg ataattctag atatttctca tcaaattctt tgtcgcttga aaagatcttg
     6241 gtcaagtccc aagttaaatt ttcaggcaat tcttcgcgtt ttggcaactg ctttacttcc
     6301 atgaaatcca ctccttttta tatccttttg tctcggtctt tcaaaataga tcctcgtctc
     6361 acatccacat gttacagcgt ctgatttttc tactaatcta tgtgtctgct tctatcaatt
     6421 ttagcacaac tgaaacttgt tttacataga aaaccggttc ctgttctatt ttttatactt
     6481 actatatttt ccaagaggat aaatgtgtgc attcatcaaa aaattgccga tagaccatct
     6541 cttcctcaat catagggaaa agccactccc ttttatattc ttttacttgg caaaaccatg
     6601 agctaaaatc aggtactttc tgttgcttta aggattcttc gtacaacatt cgataaagaa
     6661 aaacctgttc tcgtaaataa aagaaaaacc gactgttttg atagatccac ggtgatagat
     6721 acaaaaggtg cttgtgacgc aagtagcact gttcttgtat ggatactgta tttttttgtt
     6781 tgtaaatcag ctggcgcgcc aaccacttct tcccatcctc gcttaacttg tctgttttct
     6841 tcatctgatt tattttgatc tcattattag taaatatttt tttaagatca ttaccagaaa
     6901 aagtccagca gctactgaaa aaagaaatgt gacctgatac agtctctgta atatgatgat
     6961 ataaataaag tttttggttc ttaaggtcag ctttccaaca atgtactccc ctttttctat
     7021 tataatagca gcaacttttt tctatcaatg aaaattgccg ttgtccaaga aaagaatgac
     7081 caagtatcca ccatacttgg taaccgttgt tcaaataatt tttggttctt tcaaaaaatc
     7141 tctgatgaga caacctagaa cattggattt ctaatgctag attagccaat aaaatatctg
     7201 gtctctgcct caatcttggc aaataagctt ccaaaaatac aggttctgct gactgtccca
     7261 gccaatccat gaaacattcc tttaactgca agtgttcttc tgattccccc tcactaaaaa
     7321 ccgaacagtt ttcggttttt cgatgggcaa aatgagcaca ttttttcttt ccctttttca
     7381 aaaccactct tttccgacaa ctacgacaat aatacatttt ctctttttct gcatatttag
     7441 cagaacagca ttcatgatat tcattcatcg caagcagcat ttttctccct ctgaacttat
     7501 ttacgtctag ccatttattt acacgaaaaa gagccatatc gatatatgat cgatatggct
     7561 cttactataa tagtgattta tacaaaataa aatttcgtac gctcgatagc attacgcgcc
     7621 atcagacact ttccatattc actcaatgtt tctggtgtac gatcgctctt ttctgcaaac
     7681 tccaaaattc gagcaatctg attctctaca tccgcttggt tgagattctc catccagaaa
     7741 tgaacgacta ggtaataacg ctcttcaaat aagtacaaat cagtccaagc agactctagt
     7801 tcaacgtctg ttgccagctg gatcacttgc tcaaaatctt caaaagcaaa aattgcttgt
     7861 ctttcctgtt cttctagggc gcttccagat aattcttctt ccggatcgct accaataatt
     7921 tgctgacgga gaaattcgct cacgtcttct gaatcaacag cagaatcttc tgattgagaa
     7981 atttcttcat ctggcaggtt tttactgata aacagttcta atccgtctcc ttttggcaaa
     8041 acttggaaag tgacagcttc gcttcctcgg aactcctctt cgatatcaac ttcttccaga
     8101 atactataaa agaaattttc gatttctttg tgattgccaa gtaaatccaa aaatgaaacg
     8161 ccccgttctt ctaaatcttc atgacctatc agtacacgga tcgtattttc attaatgtgt
     8221 tccatttcca ttttgctaca cctcactttg ccgcaatgtt caatatagat tcattgtagc
     8281 gaaatcagct taaatgtaaa gaaaaaaaat cccgctgtta agtgaattta ataaaaccag
     8341 cagatttttt caatcgttca agctatctta tttaactata acatttttgg gagatgaata
     8401 ctatatagcc gactttttag ctaatcaatc acttctgttt ttcataccat gaccactctc
     8461 acaccctttt ttgtgcccac aataaagaac gctatttata aaaacaaaaa aacttggaaa
     8521 aactatcatc gtaatagttt ttccaagttt cattttatct tctataatcc agccatcaat
     8581 tgtgcttgac gcaattctaa ttgtcgtaca tcgcgcggca ggaatcgacg aatctcatct
     8641 tcattaaaac caacttgtag acgtttatca tcaatcatga ttggacggcg aagcaatcca
     8701 ggattgtttt gcacaagttc caatagttct tgtaacggca gatcatctaa atccatgttc
     8761 agtttttgga atactttgga acgtgtcgag atgatttctt ctgttccgtc ttctgtcatt
     8821 tgtaaaatcg cttttaattc agaactgttc aatggttctg aaaaaatatt acgttcaaca
     8881 aatggaatct gatgttcttg caaccacgca cgagcctttc tgcaggaagt acaacttggg
     8941 gaagtatata atgttaacaa cgtgcaccac tccttttcgt gataagaatc acttttttgt
     9001 aacagaagat cataataatc
//
LOCUS       DADJYG010000101         9001 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.105, whole genome shotgun sequence.
ACCESSION   DADJYG010000101 DADJYG010000000
VERSION     DADJYG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.105"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            213..1427
                     /locus_tag="JZ382_001954"
                     /pseudo
     CDS             213..1427
                     /locus_tag="JZ382_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1606..1941)
                     /locus_tag="JZ382_001955"
     CDS             complement(1606..1941)
                     /locus_tag="JZ382_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY2428130.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1925..2197)
                     /locus_tag="JZ382_001956"
     CDS             complement(1925..2197)
                     /locus_tag="JZ382_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY2428131.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2648..3451)
                     /locus_tag="JZ382_001957"
     CDS             complement(2648..3451)
                     /locus_tag="JZ382_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAY2428132.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3429..4553)
                     /locus_tag="JZ382_001958"
     CDS             complement(3429..4553)
                     /locus_tag="JZ382_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428133.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4569..5384)
                     /locus_tag="JZ382_001959"
     CDS             complement(4569..5384)
                     /locus_tag="JZ382_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2428134.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5374..6255)
                     /locus_tag="JZ382_001960"
     CDS             complement(5374..6255)
                     /locus_tag="JZ382_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2428135.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6331..7620)
                     /locus_tag="JZ382_001961"
     CDS             complement(6331..7620)
                     /locus_tag="JZ382_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2428136.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7848..8492)
                     /locus_tag="JZ382_001962"
                     /pseudo
     CDS             complement(7848..8492)
                     /locus_tag="JZ382_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8792..8950)
                     /locus_tag="JZ382_001963"
     CDS             complement(8792..8950)
                     /locus_tag="JZ382_001963"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428137.1"
                     /translation="MTVHWEGAHRPIARETQGGICEKMTEEILYQRLGSTSRNLIVSL
                     GHSMGISH"
BASE COUNT         2793 a         1906 c         1439 g         2863 t
ORIGIN      
        1 atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact aaattttacc
       61 aaaagtgagt ctctttttga gttgaaacga agaaaaagtg ttagtagaaa atcgccttcg
      121 attttccacc aacacaaaat attatagaag caaaatcaat tctaccttag atgttcttat
      181 atcactctat cagtcctatt tgacaaagag ccaagaaaaa gacgttggtc gtcgatgctg
      241 ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg
      301 ggaaagcaat tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca
      361 atcatatgcc tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta
      421 cccattggac ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc
      481 gatataaaat tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt
      541 gtcaggtatc tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa
      601 agccttctaa gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt
      661 ccatagaaga taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg
      721 tcttgcctac gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag
      781 aagaagtaga attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg
      841 ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg
      901 cactcaatga gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc
      961 aggcagaaaa actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc
     1021 attatgagta caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag
     1081 caatgatgat tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt
     1141 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1201 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1261 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1321 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1381 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     1441 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     1501 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     1561 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     1621 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     1681 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     1741 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     1801 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     1861 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     1921 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     1981 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2041 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2101 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2161 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2221 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2281 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2341 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     2401 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     2461 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     2521 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     2581 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     2641 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     2701 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     2761 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     2821 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     2881 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     2941 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3001 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3061 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3121 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3181 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3241 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3301 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3361 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     3421 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     3481 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     3541 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     3601 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     3661 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     3721 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     3781 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     3841 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     3901 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     3961 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4021 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4081 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4141 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4201 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4261 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4321 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4381 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     4441 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     4501 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     4561 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     4621 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     4681 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     4741 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     4801 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     4861 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     4921 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     4981 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5041 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5101 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5161 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5221 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5281 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5341 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     5401 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     5461 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     5521 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     5581 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     5641 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     5701 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     5761 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     5821 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     5881 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     5941 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6001 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6061 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6121 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6181 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6241 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6301 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6361 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     6421 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     6481 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     6541 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     6601 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     6661 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     6721 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     6781 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     6841 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     6901 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     6961 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7021 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7081 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7141 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7201 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7261 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7321 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7381 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     7441 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     7501 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     7561 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     7621 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     7681 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     7741 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     7801 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     7861 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     7921 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     7981 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8041 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8101 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8161 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8221 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8281 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8341 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     8401 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     8461 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     8521 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     8581 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     8641 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     8701 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     8761 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     8821 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     8881 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     8941 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9001 g
//
LOCUS       DADJYG010000102         8822 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.106, whole genome shotgun sequence.
ACCESSION   DADJYG010000102 DADJYG010000000
VERSION     DADJYG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.106"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            4..303
                     /locus_tag="JZ382_001964"
     CDS             4..303
                     /locus_tag="JZ382_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428138.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(400..834)
                     /locus_tag="JZ382_001965"
     CDS             complement(400..834)
                     /locus_tag="JZ382_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428139.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1066..1776)
                     /locus_tag="JZ382_001966"
     CDS             complement(1066..1776)
                     /locus_tag="JZ382_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY2428140.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1766..2626)
                     /locus_tag="JZ382_001967"
     CDS             complement(1766..2626)
                     /locus_tag="JZ382_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY2428141.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2631..3269)
                     /locus_tag="JZ382_001968"
     CDS             complement(2631..3269)
                     /locus_tag="JZ382_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY2428142.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3284..3583)
                     /locus_tag="JZ382_001969"
     CDS             complement(3284..3583)
                     /locus_tag="JZ382_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2428143.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3626..5110)
                     /locus_tag="JZ382_001970"
     CDS             complement(3626..5110)
                     /locus_tag="JZ382_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY2428144.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5130..5591)
                     /locus_tag="JZ382_001971"
     CDS             complement(5130..5591)
                     /locus_tag="JZ382_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2428145.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5609..6676)
                     /gene="ulaG"
                     /locus_tag="JZ382_001972"
     CDS             complement(5609..6676)
                     /gene="ulaG"
                     /locus_tag="JZ382_001972"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY2428146.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6894..7664
                     /locus_tag="JZ382_001973"
     CDS             6894..7664
                     /locus_tag="JZ382_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY2428147.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7937..8764)
                     /locus_tag="JZ382_001974"
     CDS             complement(7937..8764)
                     /locus_tag="JZ382_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428148.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2673 a         1859 c         1384 g         2906 t
ORIGIN      
        1 aacatggatt atactgttga atttgacagt gaactactta agcaatttcc acaagaagaa
       61 catttcatgt ataaagaagc aatttcccac aaagtgacag agtatttgga gcataatttt
      121 tttcgtataa agccagtgcg tactcgttcg gcaaaaggaa tatacgagat gaaagtcaaa
      181 attaataaaa aaaattatcg catagcattt gctttaaaaa aaaatcatgt gttcattttt
      241 tatattagtt cgaatttgca aaaaattgta tttgataaag aggtgagtaa aaaaataaaa
      301 tagctgagca ttggaggata gattgagtga tactaagtct atcctcttgt ttttctaaag
      361 aactaagcta gaattttttc ttttgtacgt tattatatgt tatttgtcat taccaaaaag
      421 cacctttttt cttctagtat ctatgacata agaaatacca cccatttcct ttacttgaac
      481 aaatcctact gattcataaa atttttttgt tttctcagta tcatctgtca ataattcgat
      541 ttggactggt ccataactag tgatcttctc caaagcttca ttcaaaagca ctttgcctat
      601 tccttggcgc tgatacctcg gatgaatgag gatatcttga ataaatgcga tcgtcgcaaa
      661 atcagtgatc caacgaacga ttccgataat cttcccttcc ccattacgct ttatcagaga
      721 ttcagattgt tcaaatgctc tagctaacct tgcagtgtct tttgtgtaat tcgtccaacc
      781 aacgctctta tataattcag aaatctcatc gatcataagg gaatcaatat tcattttttt
      841 cttcctttct tatacaattc tccctctaat aatacagttc tttgactatt taatctatca
      901 catataactc tcctgtaaag attctctcga ttccttaaaa ttcctctttc cttttagaat
      961 gcatactttt tgataaataa aaaggttgtg ataaattttc ttcacaacct ctgtttctct
     1021 ctatcaagtg ttatctttta attttctacc cttaccctag catctctatt gcccgtagta
     1081 tgcatacttt ccatgtttgc gaaaataatg tttatctagt aaatattgcg ggatttcaca
     1141 tatattgggg ttgatcagct cgttttcttt ggccatcaga caaatttcat ctaagatcaa
     1201 actgttttct actgctttca taggtgaatc cccccatgtg aacggtccat gtccatagac
     1261 aagtactcca gggacttcgt ttggatctaa ttttctttcc ttaaacgtct cgacaatcac
     1321 attccctgta tgaacttcgt aagcttctct tacttcttct ttcgttaatt ggcgtgtaca
     1381 aggaacctta ccataaaaag catcagcatg agtcgtgcca taagctggca aatcgcgtcc
     1441 tgcttgcgcc cacatgacgg agtgcgtgga atgagtatga gtaatagcag ttatatcttc
     1501 aaaagtttga tacaaaacaa catgtgtggg gagatctgaa gatggtttta agctatcttc
     1561 ttctattata tttcccgaca aatctgttac caccatttga tctgcttgca tgcattcata
     1621 tctgacaccg cttggcttaa tgacgatgac tcctagctca cggtccactt cactcacatt
     1681 gccccaagtc aatttgacta atccgtatcg cggcaatgcc agatttgctt caaggacacg
     1741 ttgcttcatt gctgcaattt tttcttcaca agacatcata gccagcctcc ttcaatttag
     1801 aacttaagta gtcttttgcc tgctggattt ctcttcgaaa gtcttggctt ttttcactcc
     1861 acatttcaat cagaaatgtt ccatcataat ctagacgttt caaatgtctt aataatccaa
     1921 gaaaatcaac acaaccgcta ccaaaaggaa catcacgaaa ctggcctgaa tattcttttg
     1981 ttacagggta agtatctttt aagtggatag cagtgatata atcgatccct ttctcaagtt
     2041 ctcgagccgg attattttcc ggccatgcgg acaaattgcc taggtctgga tatacttgta
     2101 gaaaaggtga atgaatctgt tctttgattt ctaaaaattt tgaaatggag ttcataaatg
     2161 gatcatccat gatttccacg gaaagaatga tgccatattt agaagcttcc tttacgcttt
     2221 cttttattcc ttcgataaag tcttctctgg aattcattga tttctcttca taataaacat
     2281 cgtatccagc tatttggatt actttgacgc ttagattatg cgccaaatgg attgcttttt
     2341 tcattagttg tttcgctgct tttttctttt ctggatcagc agaaccaaac ggaaatcgcc
     2401 gatgcgcact taaacaaatc gaatggatac gtacaccgga gctgaacatc tcttctctca
     2461 gctcagctat cttttcttca gaccaatcta atcgtgctaa ccgctcatcc gtctcatcaa
     2521 tcgacatctc gataaaatcg aagtccattt cttttacaag ggaaaaacgt tctttccatg
     2581 aaatatcttt gggtaacgct ttttcataaa tcccaatttg agccatttcc ttacccccag
     2641 atacgaatga tctcatcttt gaagttttgt gcggccttca tcggatcatt tgctgctgta
     2701 ataccacgac cagtgataaa ggtatacaca tcgatccctt caaataagtg taatgtgtgt
     2761 ggatttaatc caccagttac agaaacatta aagcctagtt caatcagttt tttgatcttc
     2821 gataaatctt tttcacccca agtttcacca gataacagcg catcacggct ttgatggtag
     2881 attacctgcc ggataccgat gttatgccaa tccatggctt gttcaaaagt ccaattgccg
     2941 tagagttcta cttgcaattc acctatttct ttttgtgctg cctccatagt tggaatcgtt
     3001 gcacaacaaa tgactgtcat ccagtctgct cctgcttttg caacattact cgccactgtt
     3061 cctccagcgt ctgcacattt cgtatcagcg actagttttt tatctgggaa catactgcga
     3121 atacagcgaa tcgctttttg tccttcctgt aagcaaagaa ttgtgcccac ttcaataata
     3181 tctactacat caccaacttt cataacatct cctaatgcat gttctaagtc attgtgatct
     3241 aacgcaattt gcaaattcgg cctgctcatc ttagttcctt cctttacttg tttttttggt
     3301 accactttgt ttcagcaaat ttttcactga tttctttgac tgacatcaca tttttgatac
     3361 cgataactgt aactcctttt ttctctgcct gagtaaacat attcaaaaaa ttgactggcg
     3421 taaaaactac atcgtattgg ccagctgaac tttttccctc tgaaatggca caatgatgga
     3481 ttttggtaat tggtacgttt agttctttca ttgctttttc cacacttctc atcatcatca
     3541 agcttgttcc tgatccattt gcacacgata ctaaaattct catcttaatc cctccattat
     3601 gagtgttgtc taatcagatt ttcttttata ctctctgctt ttctgcatat gtgtcatagt
     3661 cttctacaat caaaaaataa ccatcaggat ttttccgata ttgtaactgc ggaatagcca
     3721 ataaaccaat gacaactacg gcaattccag cataacctag gactttcatg attactgtaa
     3781 acaatggcca agctgttgcc cagtcgaaca tcccgatata accaccgtat gttgacatcc
     3841 caatccatgt agcgaataat gctgaaccgc ctacttgtat gattcctgaa agaaacggga
     3901 acaggcaagc ggctttgaat ccacctcggt tattggcata aactgctata acggcattat
     3961 caaagaacaa cggaatgaaa ccggctatga cgatcgttgg tgatttcaat aaaatcagta
     4021 aaccaatcat caaaaactgt cccaaagcac caaacaagaa cccaatcgtt accgcatttg
     4081 gcgaaccaaa gccaaaagta gcagctacgt caattcctgg aacagctcct ggtaaaatcg
     4141 tgtttgagat tccttggaaa gattgagtta gttcatcgac gaatgtccga acacctaatt
     4201 gcaagatcgc cagatatacc gcaaaattta aagaagtcgt cataatgtag aataagaaac
     4261 tctgcccttc ttgcatgaat tgcgcttgaa tcaaataatc tttgcctagt acaatcagaa
     4321 tgatcccaaa gaaaaatagc atgagtaacg aagtagcaac catattctca ttgaaaatcg
     4381 acaagaatcc tggtaatttg atatcttcaa tttttttgtc ttgcttgatt gatctatttt
     4441 tcttttcatc ccgctttttc atccactcag ctaattttgc aaatataaag attccgaaca
     4501 tttgctgatg tgccactgca aatccggcac cttctgtcaa ttcctgcgtg attcctactg
     4561 ttaagttaga agctactgtc caataacaac ctaaaatcag tcccataaaa atcaatgctt
     4621 gtatcctacc tagatcagga aagcaaaaca gtaaaatcca aaaagcggta gctgcttgtt
     4681 gaacctgaac gtttccggta gtgaaaacgg aacgcagttt cgtatatttc tgaaaacgga
     4741 ctaataagat atttaaaaca aatgcaatca gtaataaaat catcacatct ccaaaggtac
     4801 ggccaaatgt ctccattaat ccagcatcca cagcattttg tccaaaataa ggatctgtaa
     4861 ccatggcatc caaattgaac cgttccttta gtccaaccaa gattggccga aaattattga
     4921 ccaaaccacc agagccaaca gtcaaaataa agtaaccgac cgttgccttt aagaatccag
     4981 cgatgcattc atgaagcggg cgtttcagca aaatatagcc taataacacg ataaaaccaa
     5041 tcaggtatgc tggctgcgtc aagatatttg ttgcaaaata cgtccaaatg cttaacaaaa
     5101 attcccccat cttttttctc ctctatcaat taattatatt ctgccatcac aacttggtaa
     5161 tcatcgatgg agttcgtaga gatgagacgc tcaaccaatc catctgtcat taataattcg
     5221 gataattttt ggatattttc tagatgttct tctggatctt ttgctgcgag tgtaaaaaac
     5281 aaactagccg ttttctcctc attctcccct tcactttcaa aacggattgg ctgtttcaac
     5341 ttggtaaatg aaatagctgt tccaaaaaca ccatcacttt tttctgaaga atgcggcatt
     5401 gctacttgag gtgcgatcac aatataagga ccgtattttt ctacacaatg gatgatctcc
     5461 tccacatacg tttcatcaat aatttcctta tcgatcaata caagacagct ttcggcgata
     5521 gcttcacgcc aatccgtgat ttctttatct gggtaatgaa tcaattgatg gtcaaaaaag
     5581 tatttcagca agaaactccc cttctttctt ataaaaacga cggaaacggt gtatcatttt
     5641 caatcgagaa gacatcttca aatcctcgat aaaaattaaa ctcaagatca tctttgtttg
     5701 tcggatagat gaatttcccg cctacttgcc agatataagg tttaaatcca tacttcaggc
     5761 gatccttttt gaatttccaa atttcagtga tctcttttgg atcagccata aaattagacc
     5821 aaatatcgta gtggacagga atcactactt ttgcattcaa tgactccgcc atgcgcagca
     5881 tatcaacaga agtaactttg tctgttatcc ctcgtgggtt ttcaccatag gcccctaaac
     5941 agacatcgat tttgtgttca ttaccatgtt tggcaaataa attggagtaa tgtgaatctc
     6001 ctgcatgata aagattccct cctgatgttt cgaataaata gtttactgca atttgatcca
     6061 tctcctgagg catttttcct tttaatacct cttctggatc ttcacaagtg accaacgctg
     6121 tccgatcaaa cgcttctaaa gctactacat tgatgtcttt gattttgact gcatcacctg
     6181 gctttactgt aatcgttttt tcttcaggaa ttccccattt ttgccacgtg cgaaccactt
     6241 cttttggccc aatgaatgtt gcttcaggac aattttgatg aactgctgcg gctgtatgga
     6301 tatctagatg atcggaatgg atatgtgtaa cgattaaagc gtctacattt tttatcgcaa
     6361 aaggatcgat tacgaaaggc tgagtacgta aattaggctg catattttga acgccactca
     6421 tacgcatcat ctgatggcct tttttcatca tgccattccc atgactacgt tttcccgtcc
     6481 cacaccatag atcacataaa atattcgttc cttcatgact tttcagccaa attcccgtac
     6541 agcccagcca ccacatagca aaagttccag gatccactac ttctgtttcg atttcttcat
     6601 tcaagtatgt gccccactca ggaaatgttt ctactaacca cttttctttt gttacttcat
     6661 tgacagtttt actcatttgc ttgctccccc catatcgttg ttccaactta caagtaaata
     6721 ttaatgtgta tcagcatcca tgaaaaccct tttatatgac cgtttgtttt ttatatgatt
     6781 ttctttcagt aatcaatgat tgtttcagta taataaagac agatatgaat gtttaaatga
     6841 acaactagtc aaataaatcc atggatcaag atgtccctca ggaggcaaaa gtcatgaaaa
     6901 actcacttgt caatattgaa aaacgtcaaa aagaaatttt ggaactgctt cagcagactg
     6961 aacaactatc aactttcgaa ttagctgaat cccttgatgt atctattagt acgatacgaa
     7021 gagatttaaa tttattagaa gaaaaaaacg agatcatacg caattacggt tattgttctt
     7081 ataattacaa aaaccagact gattttgatc aatctggtcc tgaaaggatt aaacaagcga
     7141 ttgccagaga agctagtaaa tacattaacg attatgatac gctgtttatc aattctagct
     7201 cgactgcgtt gaaaacactg gattttcttc aatctaacca tttgacagtc attacaaaca
     7261 acgtgaaaat caattactct ccgcacaaag ataattgtag ttatatttta actggtggag
     7321 agatgcgctt tccaaaagaa gcactagtgg gcgacatcgc attaaatacg atttcttcag
     7381 tcaatgcaga catttgtatc attggctgta gcggagtcga tgtagtacat ggggtcacaa
     7441 caaagatatt aaatgaatca acgatcaacg aaatgatggt caacaagaca atcaaatgta
     7501 agattttagt cgctgatcat cgaaaaatcg gactgacatc aaagttcaag attgctgata
     7561 tttcagtctt tgattatttg atcactgata aattttgttc tgaaaaagta ttagaggaga
     7621 tcagaaatac ggggataaaa accattcaag tagattcatt ttaagaacca ggacgtaaat
     7681 atgtaaatag gattatcaaa aaaatgaata acaaatttat cgataaatga aaaacacgct
     7741 acgctcaaat tgataaagga atcccaagat gtatccatcc aatcgatacc atcataatct
     7801 attgcttctt ttataaccgt tatatgttgc ctaaatagga atcaaacaat atcgaaaaaa
     7861 ggaggtatga caaaagtcac acctcctttt ttcgaataaa cggtattcaa atcagaacac
     7921 tgatttcaca accactttat ttcaggtcgt tttcaatcac ctttaccgcg taatcatttc
     7981 cgttgaaacg cgctattttt tttgcgtcca tatatctttc taaataatca tcttctccta
     8041 attgcttatg gcaagaagca atttcataaa gcaagtattc caatgcataa gtcgatttat
     8101 gtttcttatt atactcaata cccttctcac ataattggat tgctttatca taatcctcga
     8161 ttttacggta ataggtggcg atatcataat atacaatgca gaccatcaga ttatcaaaat
     8221 cagtctcact caatatttta agtgtacgct ccacataaac tttcgcaaaa tctaattctc
     8281 cttttgacaa gtagatcatt gccacgtttg cagctgcttg taagtgatgg atatctgttt
     8341 cggtcgtttg ttccaacact tgattaaaat aaaataatgc tgcatctggt tttttcgcat
     8401 aaagatgatc aagcagtcct tgataaaata aaatttgatt taaaagatca gaattactta
     8461 ctttttcctg atcgatcgtt tgtaaaattt tataagattc ttggtatttt gatacagaat
     8521 tcaatgattg aatctcattt atttttttcc tgattttgtc ttcttctgtt tctactaata
     8581 tatcggagat ctcgacattc agctttttgc aaagcgcact taagatattg atgcctggcg
     8641 acatattctt gttttcgata ttgctgacag ttgcttgttc acaaatgcct tctgctaatt
     8701 gtacttgtgt taaccccttt ttctttcggt attcttttag taatgctcca tctactttaa
     8761 tcaaaacaat ttccttccct ttaaaaaatt atttctcctt tatatcatac acaaaaaaaa
     8821 gt
//
LOCUS       DADJYG010000103         8798 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.107, whole genome shotgun sequence.
ACCESSION   DADJYG010000103 DADJYG010000000
VERSION     DADJYG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.107"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="JZ382_001975"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="JZ382_001975"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY2428149.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="JZ382_001976"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="JZ382_001976"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY2428150.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4311)
                     /locus_tag="JZ382_001977"
     CDS             complement(3070..4311)
                     /locus_tag="JZ382_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY2428151.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="JZ382_001978"
     CDS             complement(4329..5252)
                     /locus_tag="JZ382_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY2428152.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="JZ382_001979"
     CDS             complement(5473..7965)
                     /locus_tag="JZ382_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAY2428153.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="JZ382_001980"
     CDS             complement(7972..8439)
                     /locus_tag="JZ382_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAY2428154.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8575..8648)
                     /locus_tag="JZ382_001981"
     tRNA            complement(8575..8648)
                     /locus_tag="JZ382_001981"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8612..8614),aa:Arg,seq:acg)
     gene            complement(8706..8779)
                     /locus_tag="JZ382_001982"
     tRNA            complement(8706..8779)
                     /locus_tag="JZ382_001982"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8743..8745),aa:Pro,seq:tgg)
BASE COUNT         2440 a         1867 c         1461 g         3030 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc
     8581 caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct
     8641 atgggcgcat attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa
     8701 aaaaatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac
     8761 caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DADJYG010000104         8748 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.108, whole genome shotgun sequence.
ACCESSION   DADJYG010000104 DADJYG010000000
VERSION     DADJYG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.108"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            53..1114
                     /locus_tag="JZ382_001983"
     CDS             53..1114
                     /locus_tag="JZ382_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAY2428155.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1117..2013
                     /gene="murQ"
                     /locus_tag="JZ382_001984"
     CDS             1117..2013
                     /gene="murQ"
                     /locus_tag="JZ382_001984"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY2428156.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2027..4009
                     /locus_tag="JZ382_001985"
     CDS             2027..4009
                     /locus_tag="JZ382_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428157.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4074..4520)
                     /gene="nrdI"
                     /locus_tag="JZ382_001986"
     CDS             complement(4074..4520)
                     /gene="nrdI"
                     /locus_tag="JZ382_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY2428158.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4731..6155
                     /locus_tag="JZ382_001987"
     CDS             4731..6155
                     /locus_tag="JZ382_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2428159.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6336..7109)
                     /locus_tag="JZ382_001988"
     CDS             complement(6336..7109)
                     /locus_tag="JZ382_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2428160.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7106..8011)
                     /locus_tag="JZ382_001989"
     CDS             complement(7106..8011)
                     /locus_tag="JZ382_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2428161.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8165..8485)
                     /locus_tag="JZ382_001990"
     CDS             complement(8165..8485)
                     /locus_tag="JZ382_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAY2428162.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2741 a         1575 c         1869 g         2563 t
ORIGIN      
        1 atatatactc tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg
       61 cttttcaata tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat
      121 ggcatccaat ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc
      181 ggcatatcaa aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt
      241 gatggtcgat atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac
      301 gcctttgaaa gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca
      361 gattgccgac tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa
      421 agatatcgat gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa
      481 ttattatcca cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg
      541 gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg
      601 cccactatat caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg
      661 cgctctagat ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc
      721 agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac
      781 ggaacctgta gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga
      841 cgatgcaaga gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc
      901 agcaagaaat acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt
      961 gcgctatatg ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa
     1021 tgtagtagct aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat
     1081 gaactatcaa tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac
     1141 ctaacaacgg aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa
     1201 gccgttcaac taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg
     1261 acaagtatcg aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg
     1321 atttatatgg gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta
     1381 ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca
     1441 atgactgtag ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc
     1501 aagctgaatt tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca
     1561 tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct
     1621 tgcaataaac aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt
     1681 ccagaatttt tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg
     1741 aatatgattt ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc
     1801 gatgtcaaac caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg
     1861 acagaagctg attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag
     1921 ctggctatcg tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct
     1981 gaagcaaacg gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga
     2041 actgactttg gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt
     2101 agaaagcatt gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt
     2161 aaatgtctct gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct
     2221 tcaagtcatc atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc
     2281 aggcgtgaaa ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc
     2341 atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa
     2401 acaaaaacca tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat
     2461 gattcctgca tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt
     2521 agtcacagcc ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat
     2581 cttgaaaaac gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt
     2641 ttttggtgca acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat
     2701 gaatccggaa gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg
     2761 tgggatccta ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca
     2821 taaagtagta cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat
     2881 cggtctgatt gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt
     2941 cggtgggatc aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac
     3001 attattcttg ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca
     3061 aatgattgat gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg
     3121 acaagtgggt gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga
     3181 aatgatcaaa ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg
     3241 agtaacatta cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg
     3301 ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc
     3361 tttgatccca ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc
     3421 ttatgctggt ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa
     3481 agaagaatta gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca
     3541 aacttcagct gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt
     3601 agataaagca agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga
     3661 accagaaaac ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac
     3721 gaaacacgct attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat
     3781 caacacagtt gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt
     3841 gacacctgat acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga
     3901 aacatccatg atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa
     3961 aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa
     4021 caatagaaac gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta
     4081 ttgttttctg ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca
     4141 taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac
     4201 tgattattga agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct
     4261 tcatgttcca gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa
     4321 ccatttccgc cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta
     4381 aaatcactat tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca
     4441 tgcatttttt cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc
     4501 gatatatata aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt
     4561 tttaagtttt ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga
     4621 aaaaagccgg tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa
     4681 ggtttgttat actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag
     4741 agcaacagaa aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga
     4801 ttggaggtgg cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta
     4861 ttttcagttg ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata
     4921 tcaataaaaa acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg
     4981 gccgtttcgc tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg
     5041 tagcttttgc aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg
     5101 ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag
     5161 tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag
     5221 tgccattatt tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg
     5281 ataacttttc tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc
     5341 aagtattgag ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg
     5401 tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat
     5461 tcggcttgat tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga
     5521 atcagctgca aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg
     5581 gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc
     5641 tctcatggac gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc
     5701 ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga
     5761 cgttatgtac gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg
     5821 cttacacact tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa
     5881 tgaaactttc ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg
     5941 cctttcaagt atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc
     6001 ttttcttgcc aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac
     6061 aacatatcca cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa
     6121 tggttttatt tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag
     6181 gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg
     6241 tcacagctcc tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg
     6301 aacgtctctt tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg
     6361 agatatccta atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta
     6421 gtagaaagat gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc
     6481 aacatactga tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata
     6541 ccagatatga ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa
     6601 ctattgataa agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa
     6661 ctacccaaag tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc
     6721 gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa
     6781 aggtataaat aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct
     6841 cgagaatact ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg
     6901 atcgtgcttg cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga
     6961 gcagtcattt caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct
     7021 atgagcaaaa caactaatag aacagataaa acagcagttg actttttctt gatcaattta
     7081 aataattctt gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa
     7141 tttccaatag agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat
     7201 tttgtgcaga caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct
     7261 tgattttatc aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta
     7321 gatcatttgt tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg
     7381 tacattgttt gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa
     7441 gctcgctgag taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa
     7501 tgatttttcg cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa
     7561 taacgataga cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca
     7621 acgagtagga tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag
     7681 tgtcgatagc agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta
     7741 agaatggata gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac
     7801 tagagggaga aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc
     7861 ctaaaatagc tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga
     7921 ttttgccagg ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct
     7981 tggataattc ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta
     8041 caagcataca cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt
     8101 cataaaaaaa ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa
     8161 gtgtctagtg gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa
     8221 agaaccaagt ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac
     8281 caactgcgcc aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg
     8341 cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga
     8401 ttttagaaaa accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg
     8461 ctatgattaa ttccatccat gccatactta cattcctcct taactataaa aaaatacaca
     8521 aaaagaggaa aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc
     8581 ggtggcagat tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct
     8641 catacttttt agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat
     8701 gaatgctttg caatttcata gaaagaagtt gttatttatt aatcattt
//
LOCUS       DADJYG010000105         8739 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.109, whole genome shotgun sequence.
ACCESSION   DADJYG010000105 DADJYG010000000
VERSION     DADJYG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.109"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(127..2508)
                     /locus_tag="JZ382_001991"
     CDS             complement(127..2508)
                     /locus_tag="JZ382_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAY2428163.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2498..3145)
                     /gene="recU"
                     /locus_tag="JZ382_001992"
     CDS             complement(2498..3145)
                     /gene="recU"
                     /locus_tag="JZ382_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAY2428164.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3209..3748
                     /locus_tag="JZ382_001993"
     CDS             3209..3748
                     /locus_tag="JZ382_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAY2428165.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3838..4257
                     /gene="gpsB"
                     /locus_tag="JZ382_001994"
     CDS             3838..4257
                     /gene="gpsB"
                     /locus_tag="JZ382_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAY2428166.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4279..4646
                     /gene="rnpB"
                     /locus_tag="JZ382_001995"
     ncRNA           4279..4646
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JZ382_001995"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4855..6021
                     /locus_tag="JZ382_001996"
     CDS             4855..6021
                     /locus_tag="JZ382_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAY2428167.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6067..7563
                     /locus_tag="JZ382_001997"
     CDS             6067..7563
                     /locus_tag="JZ382_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAY2428168.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7644..8327
                     /locus_tag="JZ382_001998"
     CDS             7644..8327
                     /locus_tag="JZ382_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2428169.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2746 a         1583 c         1854 g         2556 t
ORIGIN      
        1 catgtatatg ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt
       61 atttgtagct gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct
      121 tttttactat ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc
      181 atccgctccg ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt
      241 cgagctttct tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc
      301 tgctgtcgtg ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga
      361 gctttgagtc gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg
      421 aatagttgtt gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag
      481 ttcaccgccc acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc
      541 tgaaacatac tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt
      601 gacaggtgtc atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata
      661 gcccgcaaat aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc
      721 atctgtgtaa ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt
      781 cgtaccagtt ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc
      841 tggagacatg gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac
      901 gattttatta acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc
      961 tgctgccatt tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact
     1021 agagatcgca ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga
     1081 agccacttta tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa
     1141 gtaaagtgct tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg
     1201 agtcgaagtc ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt
     1261 caaatattca aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg
     1321 ggaagtattc actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc
     1381 aatctgggct tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc
     1441 gtcgtctggg taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc
     1501 gtccagatcc aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt
     1561 ttcttgtact tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc
     1621 ggattgagtt agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc
     1681 tgtttgagag attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt
     1741 cgcctgatct ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag
     1801 caatgctgtt tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc
     1861 tgtctccatc ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat
     1921 ctcttgtttc gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg
     1981 tttcagagtc tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt
     2041 caaagtactt ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat
     2101 ccgaataggg tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc
     2161 atcttctaat gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa
     2221 atcttcgaat agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt
     2281 tttatccgtc aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa
     2341 aaagaggata cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt
     2401 atgtgttcca gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg
     2461 agtaggctgt ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc
     2521 cttcaataga taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag
     2581 aaataccagt tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc
     2641 gtcctttttc cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac
     2701 agcgcttcaa agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg
     2761 ctttcatatg ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct
     2821 tggtttcttt tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag
     2881 ttgaggcttg ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta
     2941 ctttcacgat ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat
     3001 aatatttatt actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag
     3061 tgatctgatt tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt
     3121 ggccatttgg atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa
     3181 aagcggatag aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg
     3241 gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa
     3301 aaatgtttta aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt
     3361 cagtgggaat ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga
     3421 atatccggaa ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa
     3481 tgaagggaac caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt
     3541 tagccatcaa ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga
     3601 gcatacagtg ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt
     3661 ttttgaagat gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat
     3721 ggacgatctt caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc
     3781 ctatacaatg aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg
     3841 gcgaatttgg tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc
     3901 ggctatgatc ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaagct
     3961 tacagcaaag aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct
     4021 caattatcta aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag
     4081 agcgcagcag tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta
     4141 tttggtaaaa agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat
     4201 aactacacaa atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt
     4261 ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa
     4321 gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta
     4381 aggtactctt tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc
     4441 cttgaaagtg ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac
     4501 ccctcgagcg agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga
     4561 caaaaaggca gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag
     4621 caaagtacag aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa
     4681 agtaatgcgt tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg
     4741 aatcgttctt attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag
     4801 tgtggggtag gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa
     4861 aaaaacagga agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt
     4921 aaagaattaa gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa
     4981 ggaacaatgg aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa
     5041 atcgtggttg gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca
     5101 ttgccttggg aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc
     5161 aagtcgaaac tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa
     5221 cgtctccgta cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat
     5281 tttcagttgg aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga
     5341 ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac
     5401 atggcagcag cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct
     5461 gtatgtggtt caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca
     5521 ggattcaatc gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa
     5581 aatgttcgat cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt
     5641 tcagatatca atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta
     5701 ggctcttcga ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac
     5761 ggggtcatcg tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg
     5821 aaattatata aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc
     5881 ttgactagcg acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa
     5941 ttgtacaatg gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga
     6001 aaaaaagaga aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa
     6061 tagggaatga aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa
     6121 gctttaggaa tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac
     6181 cgaagtgaag tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa
     6241 atcaaagaag ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact
     6301 gccgtttttg ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt
     6361 atgagaaaat atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa
     6421 acacaacaat tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag
     6481 ttgatacctt actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac
     6541 gaacccggga tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt
     6601 ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat
     6661 cggagaatga cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt
     6721 tatgatttta gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat
     6781 ccgaatgatg cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt
     6841 ggagtgatcc acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg
     6901 tatacaccgg tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac
     6961 gagatcatta ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa
     7021 tgtgcagaag gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat
     7081 acaaaatcaa gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc
     7141 attcgttatg aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct
     7201 gaaatatgga atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa
     7261 ggcatcctac aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca
     7321 ttagggtaca tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa
     7381 aatgttctag cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat
     7441 caatatggtg catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt
     7501 aatccgcaat acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa
     7561 taaaatacaa tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt
     7621 tacttaagaa agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg
     7681 accaattagc gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt
     7741 tagctgaaaa aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag
     7801 cttacctaca tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac
     7861 agcttcgtgt attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt
     7921 ccattatcga aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg
     7981 aaggtaagat cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc
     8041 gtactgctta tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc
     8101 aaacattcaa ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg
     8161 aaaaactgtt cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac
     8221 gaagagaaac cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg
     8281 atgaaaattt tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac
     8341 agaagtggtc agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac
     8401 tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
     8461 aaatagcttc tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca
     8521 ccgtttattc ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt
     8581 caacaagtaa aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga
     8641 agatggaaat tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat
     8701 acaattgatt ttaacgaaag ggcttctatc aatggttgt
//
LOCUS       DADJYG010000106         8582 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.110, whole genome shotgun sequence.
ACCESSION   DADJYG010000106 DADJYG010000000
VERSION     DADJYG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.110"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            74..553
                     /locus_tag="JZ382_001999"
     CDS             74..553
                     /locus_tag="JZ382_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAY2428170.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            770..1861
                     /gene="phnW"
                     /locus_tag="JZ382_002000"
     CDS             770..1861
                     /gene="phnW"
                     /locus_tag="JZ382_002000"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAY2428171.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1854..1982
                     /locus_tag="JZ382_002001"
     CDS             1854..1982
                     /locus_tag="JZ382_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAY2428172.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1986..2531
                     /locus_tag="JZ382_002002"
     CDS             1986..2531
                     /locus_tag="JZ382_002002"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAY2428173.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2985..4676)
                     /locus_tag="JZ382_002003"
     CDS             complement(2985..4676)
                     /locus_tag="JZ382_002003"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY2428174.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5096..6043)
                     /gene="arcC"
                     /locus_tag="JZ382_002004"
     CDS             complement(5096..6043)
                     /gene="arcC"
                     /locus_tag="JZ382_002004"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY2428175.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6158..7177)
                     /gene="argF"
                     /locus_tag="JZ382_002005"
     CDS             complement(6158..7177)
                     /gene="argF"
                     /locus_tag="JZ382_002005"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY2428176.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7268..8497)
                     /gene="arcA"
                     /locus_tag="JZ382_002006"
     CDS             complement(7268..8497)
                     /gene="arcA"
                     /locus_tag="JZ382_002006"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAY2428177.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2458 a         1741 c         1589 g         2794 t
ORIGIN      
        1 gacgaatatt attgggcaat ccttcttttt tttgttatga tacatgtgaa atctttcgta
       61 aaggagaaag aaaatgcgca aagctcaaag acatttgctg attaaacaag tcattgaaga
      121 attcccgatc cgtacgcaag aagaactatt aaacaaactt gggcattatg gcgttaccgc
      181 cacgcaggca acgatttctc gagatatccg tgatctaaaa atcgtgaagg cacctgatga
      241 aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga actcctgaga ctaggactga
      301 ggaagaggat cggctggttc aaatgattga agacattgta ttgaaagtcg aacgagtcca
      361 ctttttgacg atcgtcaata ccttgccaga caatgctcat ttatttgcat ctgttttaga
      421 tgagatcaaa cctccctcta ttgtcagtac aattgctgga ttcgatacga tcatggtcat
      481 ctcggaaaat gaggaggacg caaaacgagt agaagaattt cttcaccatc caactgaagt
      541 atcacttttt taatcagagc acgtattcct aatatggaat gcgtgttttt ttattttcaa
      601 tattttcctt attgaataaa cctttacact ctttacaaaa atacatcatt ttttattcaa
      661 atttttcaac accttgattg aatcacaata atcatcattt atattggatg ttaacaagag
      721 cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa ggaaatcata tgtataaatt
      781 attaacttct ggaccattga cgacttctga aacagtgaaa aatgctatgc tgatcgatca
      841 ttgtacttgg gatcaagaat acaaagaaat tacacaatgg ataagaaaaa aattattgga
      901 tcttgctcaa gtatcggaag gaatatacac aacggtctta atgcaaggta gcagctcctt
      961 cggggtagaa tcggtattaa gttctgtgat caggccagaa gatactgtac tgatttgttc
     1021 aaacggcgca tatagacaac gaatgatcaa catgtgcgaa tgtctgaaac tgaactatac
     1081 cgtttatgag gtatcggaac atcaaatcct ccaagctcat gtgattacac agctgattga
     1141 agagaatccg tctatctccg cactagcaat ggtccatagc gagactactt ccggtatttt
     1201 gaataatttg gaagagattg ctgctgtcac acaagaaaga gatcttttgt ttatagtgga
     1261 cgcgatgtca agctttggtg ggataccgat ttgtgtagcc gaactaggga ttgatttttt
     1321 gatttccagc tcaaacaaat gtattcaagg tgttccaggc ttttcttttg tacttgctaa
     1381 taaagcggaa ctggaaaaac gaaaaaatgt tagccgaagt ttgtcacttg atctatatga
     1441 ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt ttcacttccc ctacccatac
     1501 ggttttggct tttcaacaag cgctgaagga actagaggca gaaggaggaa tcgaaactcg
     1561 atattcacgt tatctcgcca ataatcatgt gctacgacaa aaaatgacag agatggggtt
     1621 ccagcctttc attcaagaat atcaggggcc attcatcacc tcttttcttt accctaagga
     1681 ctggcgattt gattttaatc atttttatca gtatttaaag caaaatggat ttgtgatcta
     1741 tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga aatattggcg agatctatcc
     1801 ggaagatatt ttggaactga cagcaattat caaagactat aaggagaacg ttaatgatta
     1861 aaactgtaat ttttgattgg gcaggaacaa ctgtggactt tggctgtatg gctcctgtcc
     1921 acgcttttag aaatgctttt ctagaaaaag gaatccaact gactgacaaa gaaatccgct
     1981 aaccgatggg aaaattaaaa tgggatcata tccaacagct tctttccctc ccttctgtca
     2041 aaaaacaatg gatagctatt tatggacaac tacctcagga aaaagaggtg gaggaattgt
     2101 atcaacaatt tgaacattat ctgtttaaag atttggtcaa gcattctacc ttgaaacccg
     2161 acacattgga aacgatcgaa cggttgagaa acgctgggat caatattggt tccacgaatg
     2221 gatttaatgc tcctatgatg acaatcgtag cagaaaccgc aaaaaaagaa aggtattcac
     2281 cagacttcat ggcaactttt gaagacgtcg aagggtatgg tcgaccgttt ccttatatga
     2341 tccataaaaa catgcagtac ttcaaaaaca agtcggttga tgaagtaatc aaagtcggag
     2401 atacggtctc agatatccag gaagggaaaa atgccggagt gttgacagta ggtgtaattg
     2461 aaggtagttc gctaatgggg atttcttatg atgagtacca aaaattagag atggggaaaa
     2521 agatcgttta aaacagtctg ttgctcttag attccaacaa gccggcgctg atatttgtgt
     2581 aaacaattta aaagagttaa cagaatacat tttgtacgct gacatgaatc cagaatgact
     2641 tttatacaaa ataagaggtt gtgaaaaagc tgtcctgtat caagtaataa aaacagccaa
     2701 gcaaaaatag ctcctcatat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     2761 tgaacaccgt ttattcgaaa aaaaaaaggg agctgtgaca cgacttttgt cacagtcccc
     2821 ttatttgcaa acaaacagtg tttaagagac aacctctcca ctaaacggtg ttcattcagc
     2881 tgactctcgg tatttgctca aaaaacgata aaatttgaaa agctattttc tcttttgttt
     2941 ctctaatact cggatcagaa cgctgatttc acattctctt tttcttacat cttatctggt
     3001 gcatgaagtc ctagcaaacg aaggtcttct ttcaataata cagtcactgc ataaactaga
     3061 gctaagcgtg cttctttttg ttcatcatca gctaaaatct tcgtatgagc atagtatttg
     3121 ttgaatgctt gtgctaattt gattgcatgt ttcgcaatca ctgacggttc gtatttttcc
     3181 ccagcagaca agaccgtttc agggtatttt tgaactaatt taaccacttc ccagctggta
     3241 tcgtcattca atgcatagtc tgctgtttct gaagggacaa atcctgcttt ttccaacagg
     3301 ctgactgcac gagcatgtgt atattgcaca taaggtccag tttctccttc aaatcgaaca
     3361 acttcttcca ggttaaagtc aaatgtattc agtcgatcgt ttttcaaatc gtggaaaatg
     3421 actgccccaa ccccaacttg tttagctaca gcatctttgt tttccaaatc agggtttttc
     3481 tcgctgattt gttctttagc agattggatt gcttcattta atacttcttc taataagacg
     3541 atctttcctt tacgagttga taatttttta ccaccttgcg tgatcaaacc aaatggaata
     3601 tggtgcatgt cgtcagacca gtcaaagccc atttccttca agacagcttt caactgttta
     3661 aaatgatagc tttgttcatt ccctactaca tatagagatt gtttgaagtc gtaagtacgt
     3721 ttacggtaca atgcagcagc taagtcacgt gtaatgtata gtgtcgcacc gtctgacttc
     3781 ttaatcaacg ctggattcag gtcataagca gataggtcaa cgatctctgc acctttatcc
     3841 tcgttcagca aatgtttttc ttctaaaagt ttgacgattt catccatctt gtcattgtag
     3901 aaagcttcac cgttcaacga atcgaaacgt acttccagca gatcatagat tttattgaat
     3961 tctttcatcg attcatcacg gaaccattgc cataattgaa tagcctcttt atcgccttct
     4021 tccagacgtt tgaaccaagc acgagcttct tcatttaatt ctggttctgt ttcagctact
     4081 tcatggaatt ggacgtataa acgaagcaat tcattgattg gttctgcttt aacagcttct
     4141 tctgtacccc attttttgta agcgacgatc agttttccaa actgtgttcc ccagtcacct
     4201 aaatggttga tgcggattgg ctgataaccg attttttcca taatgaaacc gatcgaatta
     4261 ccgataactg tcgaacgcaa atgtcccatt gagattggtt tggcaatatt aggagaagac
     4321 atgtcgattg gtactgttcc ttgattcccg atgttgctat ccccataatg ttctttttct
     4381 ttcactactg tttgcaaaac ttttttgctg ataagttctt tattcatgaa aaagttcaag
     4441 taaggtccga caacttcgat tttttcaaag ttggcagaat cgattttttc tgctaaatct
     4501 gcagcgattt gctgtggcgc ttttcggtaa acttttgcta atgaaaaggc tggaaatgca
     4561 acatctccgt gttcagctga ttttggattt tctagtaatt gctcgacttg ttccagtgtt
     4621 aaatcatctt tgacagcgtc atagacagct tttgcgacaa gatctttatt attcatcttt
     4681 attcctccta aagtgttccc ttgattgtta aacaaaaaaa atctctagcg acggcatact
     4741 tagtatgcca tcactagaga cgagatttcc cgcggtacca ctccaattgt cctaaaaaag
     4801 gaccagctcc atctgatatc ggtagacgac cgtctgttta aaacagaatt ctccaagtgc
     4861 gcttcgttgt atcttcttca ttcggcttcc accacttccg aattcgctag ccaagaatga
     4921 tacaagtact ctcttgttca acaaatctta gtcttattaa actttcttga attatatcag
     4981 aaaaaaaaac gaaaggctag tcgtttcata aaaaaacaat ccagatggcc taaaggagaa
     5041 gaaataccac ctggatagct ataaatcatt tcgtaaatac gaaagactta acaaattaat
     5101 cagctacgat aatcgttcct tcttcagaag ctaaaagatt ctcgatattt tctaaagaag
     5161 tgatgattgc cttagcgttt ggttttgctt caacaaatgc aatagctgct tctactttcg
     5221 gaagcatgct tcctggagcg aattgctttt catcaatgta ttgtttcatt tctgaaacag
     5281 taacagtttc taatttcttt tgatctggtt tttggtaatt gacatacaca ttgtctactc
     5341 ctgtcaatac gatcagcaaa tccgcatcga tgatttcggc taatttttct gaagcaaaat
     5401 ctttatcgat tacggcttct cgtccttcta atccagtagc tgtttctaca acaggaatcc
     5461 caccgccacc aacagagact gtgataactc cttgatcgac cagcgtttcg atgacacgtg
     5521 cttctttaat cgagatgggt ttaggggaag cgactacttt tctccatcca cgtccagcat
     5581 cttctttgaa tgtcacagtg gagtcagcat tcatttgttc tttggcttct tcttccgtgt
     5641 agaatggacc gactggttta cttggatttt tgaatgaagg atcattttca tccacaataa
     5701 cttgagtgac taaagaaacc acatctttat cgatcccttt ttccttcagt acttcgccca
     5761 tggcgttttg taaccagtaa ccgatagatc cttctgtcat tgcaacacat gtatctaaag
     5821 gcattgccgg tgttttctca gaatcagcag cttgctgctg aatcaatagg ttaccgactt
     5881 gcggaccatt tccgtgggag atgatcaatt catctccttg ttcgataaat ttcactaaat
     5941 actttgctgt ttccattaac gcttcttgtt gggcttttgc acttgcatcc gtagacaaga
     6001 tggcattgcc ccctaaagcg acaactactt ttcgtttggc catttcattc gtcctttctt
     6061 cagttctacc gaattttcta gataacaaaa cgagccagcg ttttgtgatt ttccctcaat
     6121 gctgactcgt tttttcgtga gatgaatgtg gttatgctta aacgcgaggg ataaacaagt
     6181 tacctaatgt tgctgccatg atcgctttga ttgagtgcat acgattttct gcttggtcaa
     6241 attgacgagc atatttgctg cggaagactt catctgttac ttccatttca gtgatgccaa
     6301 agcgttcttt catttgttca ccgtaaacag tgtttgtatc atggaaagct ggcaagcagt
     6361 gcaagaagat caaacgatct gtgttgtgtg ttttttctac catttccata ttgatttggt
     6421 aaggtttcaa caatttgata cgttcttcga atttatcttc ttcacccatt gatacccata
     6481 catcagaata aaggacgtct gctccgtcta cacctttatc aacatcgtcg gtaatcatca
     6541 attgtgcacc ggatttttca gcaaagcctt ctgccatttt gacgatttct tcttcaggtt
     6601 gtaattcttt tggtgcaacg atacgcatat tcgctcctaa aattgcacct gtgactaata
     6661 aagagttagc catattgttt cgaccatctc cgcagtaagc aacagtgata ccttctactg
     6721 ttccaaagtt ttcttggata gttaaaaagt cagcgatcat ttgtgttgga tgccattcat
     6781 cagtcaagcc attccaaact ggaacaccag agaattctgc tagttcctct accatttttt
     6841 gactgaatcc acggaattcg attccgtcaa acatacgtcc tagtacttta gctgtatctt
     6901 ctgtggattc ttttttacct aattggatat catttgcacc tagatattca ggatgtgcac
     6961 ctagatcgat cgctgcagta gtaaatgcag aacgagtacg tgtagatgtt ttttcaaata
     7021 aaagcgcaat attttttcct tctagatagt gatgtgggat accacgtttt ttcaagtctt
     7081 tcaagtgttc tgaaaaatcg atcaaatatt gtaattcttc ttttgtaaag tctttttctg
     7141 ctaataagct tcttccttgg aatactgatt ctttcatatt aaacattctc ctttagattg
     7201 tttttattta agattaaaga ggaaatcccg acggcagctg acagtaagac tgccgtcagg
     7261 aaacaaatta cttcaagtct tcacggacaa gtggttggct catgcagcgt ggaccaccac
     7321 gacctcgtga caattcactt gaattgattt ctaacacttt gatgccatag ctacgaagca
     7381 attcgttcga tacataattt cggttataag tgactacaac acctggagcg attgccaatg
     7441 tgtttgatcc atcattccat tgttctcgag gtgcaacgat ctcatctcca ttaccagttg
     7501 ggatcagtac aagatcatct aatccaagag cagctttcat cgtttcgtgt aagttatctg
     7561 aatgagtaat cttgatatca tcaccatctg gttcgattgt aaagacatcc atcttaccgt
     7621 ctttactttg aattgctgga tggatagtga atttatcgtg atctaccatt gtaaatacgg
     7681 tatccaaatg catcattgca cggttgttcg gaattttgat tgccaacact ttttcaaatc
     7741 ctgaattttt tgcgaatagg tggcgtgcta atttttctaa agctttcgca ttagttcgtt
     7801 gagaaatccc aacagctact actttatcgc ttaagatcaa ttcgtctccg ccttcgatat
     7861 gatctggatt ttcacgatcc aaccatactt cgattccttt atttgcaaat cttggatgat
     7921 gtttcaaaat atattctgta aacatcgatt cacgacgacg tgcttcaaaa gtcatcttat
     7981 tgactgtcat cccatttccc attgatgcgg aagggtcgcg tgtaaagtac aagtttggca
     8041 ttgggtccat gtaaaatgga tagtcatcgt ctgctgacag atcatataga ctgctagaac
     8101 gtacttcgat atcttttgta cgaaccccag ccatgatttt atctaccatt tcttgcgttt
     8161 ccattgatag aaggaactca tgaagaccct ctcttacagc attcgatgca atatgagatt
     8221 catccaacat tttattcaag aattgttctt tgacattgcc tgcgtcaatc gcttctgccg
     8281 ctaatttttc caagtaaaga gtctcgacgc cttcattttg caatgtcttc gcaaagttgt
     8341 catgttcttc ttgagcaatc ggaagatatg gaatatcatc gaacaacaga cgatccataa
     8401 tatctggtgt caaattttcc acttcttgtc caggccgttt cagtaaaact gtctttaatt
     8461 tccctatttc agagaaaacg tgaataggtt tatccatgtt gtgttgcctc cttctttttg
     8521 attacatgta taggatatcc tccttttgta atcactttca taattttgtc tcattcaaaa
     8581 tg
//
LOCUS       DADJYG010000107         8522 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.111, whole genome shotgun sequence.
ACCESSION   DADJYG010000107 DADJYG010000000
VERSION     DADJYG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.111"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            476..1339
                     /locus_tag="JZ382_002007"
     CDS             476..1339
                     /locus_tag="JZ382_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAY2428178.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="JZ382_002008"
     CDS             complement(1468..3090)
                     /locus_tag="JZ382_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2428179.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="JZ382_002009"
     CDS             3339..3860
                     /locus_tag="JZ382_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428180.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="JZ382_002010"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="JZ382_002010"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAY2428181.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="JZ382_002011"
     CDS             5941..6759
                     /locus_tag="JZ382_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAY2428182.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="JZ382_002012"
     CDS             complement(6862..7059)
                     /locus_tag="JZ382_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428183.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="JZ382_002013"
     CDS             7228..7806
                     /locus_tag="JZ382_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAY2428184.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT         2584 a         1523 c         1671 g         2744 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 at
//
LOCUS       DADJYG010000108         8494 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.112, whole genome shotgun sequence.
ACCESSION   DADJYG010000108 DADJYG010000000
VERSION     DADJYG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.112"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..173)
                     /locus_tag="JZ382_002014"
     CDS             complement(<1..173)
                     /locus_tag="JZ382_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428185.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="JZ382_002015"
     CDS             complement(593..1714)
                     /locus_tag="JZ382_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY2428186.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="JZ382_002016"
     CDS             2042..3451
                     /locus_tag="JZ382_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY2428187.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="JZ382_002017"
     CDS             3448..4176
                     /locus_tag="JZ382_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2428188.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="JZ382_002018"
     CDS             complement(4304..5215)
                     /locus_tag="JZ382_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428189.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="JZ382_002019"
     CDS             complement(5232..6239)
                     /locus_tag="JZ382_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY2428190.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="JZ382_002020"
     CDS             complement(6236..8365)
                     /locus_tag="JZ382_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY2428191.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="JZ382_002021"
     CDS             complement(8370..8477)
                     /locus_tag="JZ382_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428192.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DADJYG010000109         8425 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.113, whole genome shotgun sequence.
ACCESSION   DADJYG010000109 DADJYG010000000
VERSION     DADJYG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.113"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            105..2714
                     /gene="clpB"
                     /locus_tag="JZ382_002022"
     CDS             105..2714
                     /gene="clpB"
                     /locus_tag="JZ382_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY2428193.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2865..3536)
                     /locus_tag="JZ382_002023"
     CDS             complement(2865..3536)
                     /locus_tag="JZ382_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428194.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(3529..4023)
                     /locus_tag="JZ382_002024"
     CDS             complement(3529..4023)
                     /locus_tag="JZ382_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAY2428195.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4043..5263)
                     /locus_tag="JZ382_002025"
     CDS             complement(4043..5263)
                     /locus_tag="JZ382_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428196.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5410..7245
                     /gene="lepA"
                     /locus_tag="JZ382_002026"
     CDS             5410..7245
                     /gene="lepA"
                     /locus_tag="JZ382_002026"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY2428197.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7339..8421)
                     /locus_tag="JZ382_002027"
     CDS             complement(7339..8421)
                     /locus_tag="JZ382_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428198.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2706 a         1614 c         1694 g         2411 t
ORIGIN      
        1 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
       61 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
      121 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
      181 atcaagatat cgacattgcc catgtatgga aaatcttttt acaacctaat cattttggtc
      241 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
      301 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
      361 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
      421 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
      481 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
      541 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
      601 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
      661 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
      721 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
      781 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
      841 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
      901 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
      961 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
     1021 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
     1081 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
     1141 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
     1201 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
     1261 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
     1321 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
     1381 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
     1441 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
     1501 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
     1561 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
     1621 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
     1681 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
     1741 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
     1801 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
     1861 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
     1921 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
     1981 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
     2041 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
     2101 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
     2161 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
     2221 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
     2281 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
     2341 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
     2401 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
     2461 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
     2521 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
     2581 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
     2641 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
     2701 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
     2761 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
     2821 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
     2881 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
     2941 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
     3001 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
     3061 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
     3121 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
     3181 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
     3241 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
     3301 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
     3361 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
     3421 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
     3481 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
     3541 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
     3601 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
     3661 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
     3721 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
     3781 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
     3841 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
     3901 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
     3961 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
     4021 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
     4081 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
     4141 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
     4201 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
     4261 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
     4321 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
     4381 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
     4441 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
     4501 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
     4561 cagctaccca atagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
     4621 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
     4681 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
     4741 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
     4801 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
     4861 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
     4921 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
     4981 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
     5041 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
     5101 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
     5161 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
     5221 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
     5281 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
     5341 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
     5401 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
     5461 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
     5521 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
     5581 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
     5641 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
     5701 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
     5761 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
     5821 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
     5881 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
     5941 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
     6001 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
     6061 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
     6121 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
     6181 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
     6241 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
     6301 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
     6361 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
     6421 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
     6481 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
     6541 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
     6601 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
     6661 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
     6721 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
     6781 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
     6841 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
     6901 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
     6961 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
     7021 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
     7081 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
     7141 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
     7201 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
     7261 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtggt
     7321 ttttttcggt ggcaaaaatc aagaattctt tctaagattt tccatgatat cgaccgtttc
     7381 atttctcatc ttatctgtta catgcgagta agtatccatc gtaattgaaa ttctgctgtg
     7441 gcccaatcgt tcagagattt ctttcatttt tgcgccattt tcgagaagaa gtgtagcatg
     7501 agtatgtctg agagaatgga agttaaaaga gagggagagt gcattcgata tcctcctagt
     7561 attccatttc actacacttg gcgtaactag ctcaccgtct tccttcgtac atactgcatt
     7621 tgaatcaatg tagagctttc catacttcat tcgattttct aattgttgtt tcttatgttt
     7681 tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt cgattggaac tgcttgtctt
     7741 tggcgtacca tatacccatg cgccgtcatt ctttaccatt tgtttctcta cagtaattgt
     7801 tccatttgaa aaatcgacat catcccacgt cagaccacaa acttcgccaa cgcgcattcc
     7861 cgtataaaat ccaatattca aaggaatata gaaaggatgt ccttcaggag taatttctag
     7921 catatgatca aagtcctcaa gagaaatgat ttttagatct tttttagtcg ttggtcgtgc
     7981 ttcatatttt ggtattttta catacagcat aggattttgc ttgattaatc cccaaggata
     8041 aaccgccata ttcagcgcat tcttaaggac agagtgagta atagtcattg ttttcttcga
     8101 gtaacccttt ttaaattcag cattgatgaa attttgtaaa agagcagggg agagatccgt
     8161 aagttttttc tttcctaaat aaccgtttat atgatttttg atggtaaatc ggtagttttc
     8221 ataggtattg tattttagat ttagtttaac gtattcctcc atccaaaaat caaggtattg
     8281 ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt tcgtcaatat ctgataaaac
     8341 ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg ccaactttct ccactttttt
     8401 tcttgagcca tcatcattga tatct
//
LOCUS       DADJYG010000110         8323 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.114, whole genome shotgun sequence.
ACCESSION   DADJYG010000110 DADJYG010000000
VERSION     DADJYG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.114"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            18..491
                     /locus_tag="JZ382_002028"
     CDS             18..491
                     /locus_tag="JZ382_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAY2428199.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            500..727
                     /locus_tag="JZ382_002029"
     CDS             500..727
                     /locus_tag="JZ382_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428200.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1363..1734
                     /locus_tag="JZ382_002030"
     CDS             1363..1734
                     /locus_tag="JZ382_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428201.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(1990..2232)
                     /locus_tag="JZ382_002031"
     CDS             complement(1990..2232)
                     /locus_tag="JZ382_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY2428202.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2264..3166)
                     /locus_tag="JZ382_002032"
     CDS             complement(2264..3166)
                     /locus_tag="JZ382_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAY2428203.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3179..3367)
                     /locus_tag="JZ382_002033"
     CDS             complement(3179..3367)
                     /locus_tag="JZ382_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAY2428204.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="JZ382_002034"
     CDS             complement(3381..3944)
                     /gene="amaP"
                     /locus_tag="JZ382_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAY2428205.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3982..4875)
                     /locus_tag="JZ382_002035"
     CDS             complement(3982..4875)
                     /locus_tag="JZ382_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2428206.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4953..5891)
                     /locus_tag="JZ382_002036"
     CDS             complement(4953..5891)
                     /locus_tag="JZ382_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAY2428207.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5925..6275)
                     /locus_tag="JZ382_002037"
     CDS             complement(5925..6275)
                     /locus_tag="JZ382_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAY2428208.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6308..7210)
                     /locus_tag="JZ382_002038"
     CDS             complement(6308..7210)
                     /locus_tag="JZ382_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY2428209.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7203..8060)
                     /locus_tag="JZ382_002039"
     CDS             complement(7203..8060)
                     /locus_tag="JZ382_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY2428210.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2293 a         1600 c         1405 g         3025 t
ORIGIN      
        1 ataaaagagg gataaaaatg aaatttaaaa cattgctatt aaaatttgtc gttttaggta
       61 taagtgccgt atttattttg ttcggcgtac ttttttatac tcagatcgct acaagcgaaa
      121 aaggattcaa ttttgattgg cagaccacat tatttgtttc tgtcatttac tttgcccttc
      181 tcctagggat cgttattgct tatttcttat tgaaactatt gaaaaaaatc gaagataacc
      241 aagctttttc cactcaaagc ttaaacatac taaaaaaaat cgaacgaacc attttgttta
      301 ttggtttcgc cttttttggc ttacttccta aatttatgaa agtagcggat ttagacgatg
      361 ctccaggttt gatgttggta ggaatcgctg tcgtattcat tccttttgca gtatacacat
      421 taacaagcgt gctggaaaaa ctgcttgaac atgcgatttt acttaagaca gatcatgatt
      481 tgacagtgtg aggtgcgaca tggcaattat cgttaattta gatgtagtat tagctcaaaa
      541 aaaaatgagt gtgactgaat tgagtgagaa agtgggcatt acgatggcaa acatctctat
      601 tctaaaaaat ggaaaagcaa aagccatccg tttttccact ctcgaaaaaa tctgccaagt
      661 tttggagtgt caaccaggtg atttgttgac ctataaaaat gactcgaata ctataccaga
      721 agaataaaat aaagtagaac aaacagtaaa taaaaaataa tctgaatgat agatgtgtga
      781 gacttttaga agttatttac gtctattatt cagatttttt atttcgattg aataccgttt
      841 tatctataaa taagatatgt actgactcat acaaaggggg ccttttcttc tccatgtact
      901 atcacttttc ctgatacaac tggtttcctg ttacgttgta gttggatctg cttttagtat
      961 aatactttat tcattccacc agactgatag cagtttgagc agtctaaata atcatgtagt
     1021 attacaggcc attctgtttc ataccttgaa gtgcttaaaa cttcagctaa tggcccattg
     1081 tgttaactct tactccattc ttttttagta agctcccata tatactgtaa agccttctat
     1141 atattatgtc atgttgcttt ttaacattta ctatgtagtt ttgataaaat gatttgaata
     1201 acagtgatgg acaattcttt tttattttta acattatttt gataataatg attatttatt
     1261 taaaaataca ttagataaca tttgttacga cacgaaaaag cttcacaaac aaatatcgtt
     1321 attttaaaat gagggcgaac tcacacaatt aggaggaata gtatgccatt tattgtttac
     1381 ttttttcttt cattactcgt gatctatatt ccattaccaa catttttcct gctcgtcaat
     1441 cgtctagcag agcaacatga tacgattgct gctcttacga aactttgttt acttctattt
     1501 gtttgtctag attactttac gagctattat tggctaaaac agacaagagt aaaaaagaga
     1561 tattatttct tccttgtctt tttcaatctc tttgagtttc tgatccattg ctttttactt
     1621 catcagtatc atacgatcca tttgtggatc atctgggttt tcaaagtggt cgtattattt
     1681 tgtatgttca cctttcctat ctcaagaaaa tttagggagg cattgtttta ttaaagaagc
     1741 ttctctgtat caataaagac acatttaagg aaaaactttt aaaaatctaa ttgggaattt
     1801 gcccgcataa ataagaggct gtgacaaaag tcttgtcaca gcctcttatt ccgtataaac
     1861 agtgttcaaa agcttgcatc ttcgataata tgcccaacca aatgaaaaat atgagaagct
     1921 atttttgttt ggctgttctt attacttgat gaagaacagc tttttcacga cctcttttta
     1981 aaagtctttt tacagttttc gagcaaagaa tgatacgatt agtactaaaa tgatggcacc
     2041 aatcaatgat ggcacgattg ccattcctgc aatacttggt ccccaagaac ctaataatgc
     2101 ttgaccaagt gctgatccaa ctaaacctgc tacgacattt gcaataatcc ccatcgaaga
     2161 acctttattc gtgattgctc ctgcaattgc tccgataatt cctccgacga taagtgacca
     2221 aataaatccc atgtttcgtt cctcctaatg ttttacttcc ttattactga acccgtggtt
     2281 ctgtattttc ttttactttt tgtgcgccgc ttccagctgc tgattttact ttatctgttt
     2341 gattcgaagc aaattcaccg gtttttttcg ctgcgtcagt tactttgtct tgcaccgttt
     2401 cttgatcttt ttcaaattct tcttttgttt tgatgtccac tacgttagcg ttgacttcga
     2461 tcacttctaa atgtgtcata ttttttactt ctttgccgat tacttcttgc atttcttcaa
     2521 agattttttc gatatctttg ccatattcca caatgacatc catatcaact gctacttgtt
     2581 ttttaccgac ttctgtttcg atcccagccg ttacattatc tgtattcaca agttttcctg
     2641 ccacattaga gaagaaacca ccgtttgcag ataataaacc gtctacttgt tcaattgcga
     2701 ttccgatgat tttttgcaca actttatctt cataattcag ctcaccgcgc attgaatctt
     2761 cctgttcaga atcatgcttt ccttggttat ttgtatgatc agaaccaagt tttccttgtc
     2821 cgtttgtgtg gtcagaacta agtttttctt gtgctttcgc atggtcagaa ggatgttttt
     2881 gctcttccac aggtttagaa ggatcgattg catcatgttg ttctggaact ttagattctg
     2941 gagttcctgg atgatgaggt gcatgctttt ctgcatcatg tttcttctct tctactcctt
     3001 gttgaacgtt agggactact ggatctggat gtgcagatac tggtgctgtt ttgttctttt
     3061 catcgttcac accatttgat tggtttggtg tcgttgggtc tggatgtgca gacactggtt
     3121 tttggttgtt cattttattg ttattatttt tgttattgtt attcatggat gtttcctcct
     3181 aagatttaaa atagtgatct aagattttcg tttgtttcag ataaaaaccg atagcgacgc
     3241 cgatcgctgc catgatcaag atcaataagg tcttgaaaaa gcccacggtg atccataaaa
     3301 cggctaatat caatccaagt accccaaaga tgatagggag cttgtatgtc gaaaatagtt
     3361 ctttcatttt tggtcccccc ctattctact cgtttgcggt cattgcttcg tttagctggt
     3421 tcttcaaagc ctgtgtattt caccgatact ttcactggct cttttgttcc caatatgtgt
     3481 tgtacttcat tttcgatttc tttcatcaat gttcctgttt taccaatgag tgctgaagtt
     3541 cgtttcaact ccccttttac attgacggcg atcttgtttt tcgttgcttt gacatttact
     3601 tttggtgaat ggatgaattc atcttcatgc aagtgagaac gaaccattcc ttcaattgct
     3661 tttttatcca acgtcaactc cccatcacca tgtttcagta gaaagtttcc gattgttttt
     3721 ggataaaaca ggaaaacaag taacgtgata attgtcaaaa tagcaaatgc aacggcaagc
     3781 caaaatacat attgttgcac gtatgaatta gtgatggaaa agaatcgaaa cttctctagc
     3841 tgaaaaggca atggataggt tgcacttaat agtatcgcaa acaaaagcac agatagcaaa
     3901 agtaatgttc cgatcactcc gatcgttttt acacctttat tcattttcgc acctcctcat
     3961 aggggtgaaa cgatcaactt cttataagtt gatcgcttct ccaccagtta ctccgtaaat
     4021 ttgggctgta acataacttg ctttattcga tgctaagaaa acataaactg gtgctaattc
     4081 tcctggctga cctgcacgtt ctagtaaact ttgctgacca aattcaggga tcgaatcaga
     4141 aggttgtcct tcgtctagtt gtaatggtgt ccagattgga ccaggtgcta caccatttac
     4201 acggattcct ttaggtgcaa gctgttttgc taaaccgatc gtaaagttag cgattgaagc
     4261 ttttgttgct gcgtaatcta ataaatgttc gcttggattg aatgcttgaa cagaagttgt
     4321 tgttacgatt gctcctcctg ctgggatatg cggaacagct tctttcacta aagcaaacat
     4381 agcgatgatg tttatcataa atgtgtcttt gacttgctca agtggtaact cagcaataga
     4441 ctgacaagaa atttgctgtc ctgcgtttag taccatcgta tctagcccgc caagttcact
     4501 cacggcttta tctatgattt ctttaggtgc ttgttcgtca cgaagatcaa aaggtagtaa
     4561 gacaatctta cgtcctgcaa ctttcacgta ttttgctact tcctctgcat ctttttcttc
     4621 tcctgggaag aattgaagag cgatatctgc cccttctcta gcaaatgcaa tcgctgctgc
     4681 acgaccgatc cctgaatctc caccagtgat tagtacacgg cggttttcta gttgattatt
     4741 gccttcatag gattcttctc cacaatcagg aacaggaatc atattctgct gtaaagctgg
     4801 tgtatcctga tcttgtttag gaaatttctc cgtatggtac aagtttcgcg ggtcgattaa
     4861 ttttggttct gtcattgctg tattcctcct agtttttaaa ttgtattgcc aaaacaaatt
     4921 tctttgtttg atacctgttt taaatagatt ctttaatttg tataatcttt ccggtataaa
     4981 tggtaaccta aaactactgc tgctatcaaa gagccaaaga ctacgagtat aaagcctaga
     5041 gcagaacttt tacccggtaa tcctcctgta ttcattcccc aaatccctgc gaacaacgcc
     5101 ggaacggaaa tgattaatgc agctgaatct agatatttca tgatatgatt tagattattg
     5161 tccatcatcc cgttgaatag atcaccaata ctctctaata aatctcgata gatctcgatc
     5221 attttttctg tttgtcgctg tctcaaatgg acatcataga gtagctgttc atcgtctaaa
     5281 cgtttgatga aagtttcaga attccataat tgatccattg tttcccgttg atctcttaag
     5341 gtatgatcga tatatacgat gtctctttca agatctgcct gtttatacag ttcttcattt
     5401 tccgttgttt tacgagctgc tttatccaaa gcgtcaattc gctttttcat ctctcttagt
     5461 tctttcacgt aatgggtgta aatatctaat aatgtatacg tgatgatctt ttcaaacgag
     5521 tttaaatgat ttccatgctt tgtcagcagc cgatccacaa attcggtttt tctatcgata
     5581 caagtaatca agagattatc ggaaagtaca aaagatattg gcgttaattt tttttcgata
     5641 cgattatctg actgatctag attaatcact accaaagaaa atggatgctc tagtttcgtc
     5701 tcataaaggc gctccatccg agaaacttct tccggatatt cggttcctac gaaaatatca
     5761 tcaggaagct cgaattcttc gattgcttgc tcaatctctt cgggctttgc actatcaagt
     5821 acgagccaat tatatttttt ttcatctgtt tctattacat tcttttcttc atccacaaag
     5881 tagtatctca atgtatacct cctttttgga ctttctcgaa actattagct gaagaagcag
     5941 ttctttaatt tgatattata atcgagtact gcgatttcat tttctaatga aaagctgtca
     6001 agctgtttgt tgaagtccat gttcaatcgt ttaccgaaat aattttgctc taatttagat
     6061 gtatagatgt ccaataatgg atgctcgatt ttagtcgcat attctccaaa ttccgaaaca
     6121 aaaggaatga tttgaaaaaa gctgcctttt gcaaaagcac ctttgtccct tagaaatgga
     6181 tcggaatcat tgataagcgc gagtgcgatt tttgtatttt ccccaacctt ttcaattaac
     6241 gtctctactg catttttaga cattgtaatt tccattccta ctccccctct tcagttgttg
     6301 tcttctatta tttaacaact acatctgttt ttggttgaac ttttgcatat tttggatatt
     6361 tagggaattt agctacacaa cggaaagccg tcatccctgc atatttttcg taatatttct
     6421 tcgttggccc ttcaaaatcc ttgctccacg gtttcacatg tcctgaaaaa tggataatgt
     6481 aaggattgtt gcgagtttct tcgtattcac gttcacctgc tgctgttgga tgtttttttg
     6541 cttttgccat gatatagcct tgtgcattcc aacgcggatg aagtggtaac caacggtcat
     6601 gtaagatagc atttaatgca tcttggtcat ggaatcgtaa tttttctgga ttatgttcga
     6661 tgaaatctaa tactttttgc gtaatatttt catctaacca ttttttcaca ttgatcagca
     6721 tcaaacctga gttgaaataa cgcattgatt tagctgaaat tttcattttt tccagtcgtt
     6781 gatggaaccc agcgtcttca acagctgcta cgattgaatc accgaagtca agacgccata
     6841 atttagagat atcttgtaaa gcgatcatgt cgcaatccat atataagata cgttcaactt
     6901 ctgtgccgcg gaacaattcc ggaatcgcaa tacgataata agctgtttct ggaatacgat
     6961 cgctgattac cacatttgta aagaattctt tattgatttt caagaattcc acatctgagt
     7021 tcattcgtgc gtttttcact gagaagcgta aaagctgttt actttcaaaa tcgatatcat
     7081 cgtcaatcac atagaaccga acaaatttag acgggttgca attatctaat actgagacaa
     7141 ataatgcagc taaatgtggt acgaattttg tgttacaact tgcgactact gctatttctt
     7201 ttttattcat tgatctcgcc tactttcgtt agagtactgt gtgctaattt ttttaagtat
     7261 aaattggtat acggatgatc tttcaacgta ttccaaggtt tatcatgtcc tgtaaagtga
     7321 acgatcgaag gtttttcatt tccttcttta tactgacgtt catatttttc atttggtgct
     7381 ggatgacgtt caaaaatcag cgaagtctgc atgttccatt ttggatgaag ttgttcccaa
     7441 tcttcataaa gtacagcatt caaagcatct tggtcgtgat agatgattcg atcgccattt
     7501 tcactgaggt aatggatagt tttttctgta atttcttttt tattccattg atcaatgtca
     7561 atcaccatca ctcctgaatt gaagtacaag tcatcggaat cgattcctaa tctttccaaa
     7621 gctttcactt ggcctggatc gataatagcg ccgattgttt ttccatttaa cggctcatca
     7681 tatagtttta cgatatcgtc tagtactaac acatctgaat ctaagtaaag gactttctta
     7741 taatcctctt tagctaataa gtttggtaaa gaaattctta agtaagcggt agttgtgata
     7801 tggtcactga ctaggaagtc ttcgtagata tctttttcca ctgtcaggaa ttggatactt
     7861 gcattacttg aatatttatt taccgtttct tccaatcctt ctttactata ttctgataga
     7921 ccatcgtcaa tcacataaaa ttttatgcgt cttgttttat tgcaattttc taatgcagtt
     7981 gcgatcatca cgctcaagta aggagcataa ttttcgtctg aagcagttac cacaggaact
     8041 acgccgtatc tcttctccat caaaatcact cctttttcta atctttttct aacctgttct
     8101 acatctagaa tattcctcat ttggttttaa agcaaaatat atgctttgtt ttcattgaat
     8161 attttaaaat ttttttatca caaaacgcat cggtaagcgc taactattcg tttttcatgg
     8221 ctttttattc aaaggtattg accaaaaact actatatata gccacctttt tcttaagaat
     8281 gttttttgaa aaaattcata atgacaaagc aagttagttt cat
//
LOCUS       DADJYG010000111         8236 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.115, whole genome shotgun sequence.
ACCESSION   DADJYG010000111 DADJYG010000000
VERSION     DADJYG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.115"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..426)
                     /locus_tag="JZ382_002040"
     CDS             complement(<1..426)
                     /locus_tag="JZ382_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY2428211.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQA"
     gene            complement(556..1266)
                     /gene="budA"
                     /locus_tag="JZ382_002041"
     CDS             complement(556..1266)
                     /gene="budA"
                     /locus_tag="JZ382_002041"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAY2428212.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1279..2943)
                     /gene="alsS"
                     /locus_tag="JZ382_002042"
     CDS             complement(1279..2943)
                     /gene="alsS"
                     /locus_tag="JZ382_002042"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAY2428213.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3151..3813)
                     /locus_tag="JZ382_002043"
     CDS             complement(3151..3813)
                     /locus_tag="JZ382_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAY2428214.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3823..4638)
                     /locus_tag="JZ382_002044"
     CDS             complement(3823..4638)
                     /locus_tag="JZ382_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2428215.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4899..5342)
                     /locus_tag="JZ382_002045"
     CDS             complement(4899..5342)
                     /locus_tag="JZ382_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAY2428216.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5373..5486)
                     /locus_tag="JZ382_002046"
     CDS             complement(5373..5486)
                     /locus_tag="JZ382_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2428217.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(5476..5814)
                     /locus_tag="JZ382_002047"
                     /pseudo
     CDS             complement(5476..5814)
                     /locus_tag="JZ382_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5802..6179)
                     /locus_tag="JZ382_002048"
     CDS             complement(5802..6179)
                     /locus_tag="JZ382_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY2428218.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6403..7596
                     /locus_tag="JZ382_002049"
     CDS             6403..7596
                     /locus_tag="JZ382_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2428219.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7762..8184)
                     /locus_tag="JZ382_002050"
     CDS             complement(7762..8184)
                     /locus_tag="JZ382_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY2428220.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2411 a         1670 c         1403 g         2752 t
ORIGIN      
        1 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
       61 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
      121 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
      181 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
      241 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
      301 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
      361 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
      421 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
      481 taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
      541 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
      601 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
      661 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
      721 tgtatcatcg ataaaatgca ggtgaaatcc cgctgcagca actccttgga ataattcagg
      781 cgtaaagaac ccgactaatg ttccttggat attgctttgt tggaattctg gttgagactc
      841 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
      901 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
      961 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     1021 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     1081 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     1141 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     1201 tccatccatc aagccggcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     1261 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     1321 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     1381 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     1441 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     1501 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     1561 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     1621 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     1681 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     1741 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     1801 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     1861 tccttttgat tgaacaatgt cccgctccgt taattttgct tgaagagatg ctaaatattc
     1921 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     1981 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     2041 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     2101 gattggatca tagccaattg caataactag atcacttcgt ttcaaaagca tatctcctgg
     2161 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     2221 tccagctgct tggaatgtct ctacaacagg caatgctgtc ttttcaacta attgcctgat
     2281 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     2341 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     2401 atcgctcaat ggcttgatga cattaccttg gacaggtgca tccactacat cttgcgggat
     2461 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     2521 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     2581 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     2641 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     2701 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     2761 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     2821 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     2881 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcagc
     2941 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     3001 aagtgataca gtttctaaac ctgtttcata caaaagtata gcacgtttta tttcatttgt
     3061 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa aaaactgtca
     3121 ttcttaattg aaagtttcaa tttataaagt ctacagatcc atagttctca tcaatttacg
     3181 attgttgaag taaatcaaac caaatacgat agaaggacct agtagaatac accattgggg
     3241 ctttacgaaa aagaagagaa acaaacttgt caatagtaaa aaatctagta aactcacata
     3301 ccattttttc aacgtatagt agaacgctat ccgtagaact tgatttggtt tcgacgctgg
     3361 atttctcacc tgaaaatacc agcagttggt gctaaaggaa actgataaac aagcaagtaa
     3421 aacaaacaac ggaatcaacc attttccata agaaaaacgt ataaaaaaga aacaatcaaa
     3481 aatcattatc acactaacta tccaagcaaa tagccaataa aaaaatccct ttagccaaaa
     3541 ttggcgaaac atctttatga acaaacgtac tggtgaaaaa ctattttctt ttgctaattc
     3601 ttctgtcata cctatcatca cgatgcaacc tggaccaata aaaagtaaag aaaaaataaa
     3661 ggaaaagaca ttccttccat ctatcgacaa aaggaacgca gctgctatat acaagatatt
     3721 gacacaccaa aaacaaaggc tacttgccaa tgattgatac aaccaataaa agaattccat
     3781 ataaacattt ttgtcaaaac gctgctcttt cataatcacc tcctattcct tcattcctga
     3841 tgaagcgatc ccttctacaa aatatttttg catcgctaaa aatataaaag caacaggaag
     3901 aacagaaaca actgaggcag ccatgattaa tgcgtactct gcatcaaata gcccaataaa
     3961 cattttcaac cctaactgga ttgttttatt ttccgtagaa gacaaataaa tgaatggccc
     4021 catataatcg ttccatgtat taacgaatgt gatgatcgtc aaactggcaa tcgccggttt
     4081 tgtaagaggt aaaatgatct tgcgatagat tccccactct gataagccat cgatccgcgc
     4141 actctcacat aaagaatctg ggatactact gtaatattgc ttcaacaaga acacaccaaa
     4201 agcattgaac gcttgtaata gcaccaacga ccacaacgta tctgttaaac ccacttttct
     4261 catcataatg aactgcggga tcatataaga ctgccaaggg acagcaatcg tgccgatata
     4321 tgctaaaaat aaaatatcgc gccctttaaa atttagttta gaaaacccgt aagcagcaaa
     4381 actagaagta aacaactgaa tcaacgtgat cacaacgctt aaaaaagctg tattcttaaa
     4441 aaatgtcact aaaggaatac gagaccaaat aaccagataa ttctctaaat gccaagtttt
     4501 agggatccat tgaatcggaa tcgtgaacac ttcattattt gctttgaatg aagcagaaac
     4561 catccataaa aaaggaatga tcgtcaataa agctaataca atcagcagaa tcatacccaa
     4621 ccattgaata aagggcatgt tttttgaacg aatttttgct ttttttcttt catttcgaac
     4681 acctccaaag ttataatctt catttctttg taatgattac aatccagaac aacggttatt
     4741 atagctgatt catttccgtt cttcttattt ttgtaagcgc ttatttaaca agttatgtaa
     4801 atctctttat ttttttgatt taaaaatgaa aaaatacaaa agcaagactg tcatacgtct
     4861 cgcttttgta ttgaaatttc ttcgattaga gatacttatt actgttcaaa agcttctttg
     4921 aaacggtgaa tcaattcatt gactacaggt gataaaatcc gattcttttt ccagactagc
     4981 atgcagttcg tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt tgaatgatga
     5041 cttacagcac cttctatgac taaagcagca cctacctgat tctcaattag cgcaaataca
     5101 ttgaaaatca aattgtacgt tcctgtgatc agcaactgct ccttttttat gcctaaccag
     5161 tcagcaaaca ttcgctgtat ttctggacgc tttgagatca aaagaggtac attcgcaaag
     5221 tcttcttttg ttatcacgtc tttttttgct aaaacagaat tttctaaaat gagcaatccc
     5281 catttttcct ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac tggatctagc
     5341 aaaatcgcta aatctaacag gcttttttct agtcactccg tgatatcatc acttgttccg
     5401 ctgtggatat gaaattgaac gctgggataa tcacggatca acttttctag taatagtgcc
     5461 aatgtattcg aactatcagc ttccacacag ccgatcgtaa atcggccgct gaataattgt
     5521 ttttttctgt tctcgaattc ttgagtggtt tgattcgtta aatctaaaat ttcttctgct
     5581 cggctttttt agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa
     5641 cagttctgtt cctagttact cttctaactc cttcagttgt cggcttaagg ttggttgtgt
     5701 aagatgtaga acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata
     5761 ttttagtaag cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt
     5821 ttatttctag ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacat
     5881 tcttgcattt cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc
     5941 cgctcaaata cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg
     6001 atcgataatc cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct
     6061 gtataatcac ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca
     6121 taatagcgca atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc
     6181 tgatcactct ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac
     6241 tttaagtgta ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc
     6301 ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata
     6361 cttttaacaa tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga
     6421 aaggtattag tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta
     6481 gctttttcat tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc
     6541 atttcaacga tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct
     6601 gataaatatg gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct
     6661 ttattgatgt attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt
     6721 gtaggagcgg gaggagaata tggggcgtgt atgtccttga tttccgaaag tttttcaaaa
     6781 aaacaaattg gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg
     6841 gctgcgattt tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta
     6901 ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa
     6961 ttcaaatctg tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa
     7021 catcttgctt ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat
     7081 tttgggttaa tgaattggct accttcaatt atgcaaaatc gattagggct ttcggtttct
     7141 gggtcttcat tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt
     7201 ggcataatca tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca
     7261 tccttatctg tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct
     7321 gtgatagtcg gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt
     7381 ttgtatccaa cagaaatccg cgcgacggcg aataatttcg ttatgaacct tggacgagct
     7441 gttggtggtt tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc
     7501 gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact
     7561 ggtgttacag cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga
     7621 aagaggtgtg gaaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag
     7681 acactgtttc cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa
     7741 tttccttcta tctggcggcg actattcttt gacgtccgtc atttcaaagt actttactaa
     7801 taacttagca taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc
     7861 caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct
     7921 gtagaaatga agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg
     7981 ccgattattc tcattcttca aaatgacctt atcaggatat tgttggaaca attcttctaa
     8041 atctgttaga taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc
     8101 tattgaacac caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga
     8161 aacttttgtt aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat
     8221 acaagaaaaa aacgga
//
LOCUS       DADJYG010000112         8201 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.116, whole genome shotgun sequence.
ACCESSION   DADJYG010000112 DADJYG010000000
VERSION     DADJYG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.116"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(14..679)
                     /locus_tag="JZ382_002051"
     CDS             complement(14..679)
                     /locus_tag="JZ382_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAY2428221.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            655..828
                     /locus_tag="JZ382_002052"
     CDS             655..828
                     /locus_tag="JZ382_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAY2428222.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(873..1610)
                     /locus_tag="JZ382_002053"
     CDS             complement(873..1610)
                     /locus_tag="JZ382_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY2428223.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(1612..3114)
                     /locus_tag="JZ382_002054"
     CDS             complement(1612..3114)
                     /locus_tag="JZ382_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2428224.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            3373..5367
                     /gene="uvrB"
                     /locus_tag="JZ382_002055"
     CDS             3373..5367
                     /gene="uvrB"
                     /locus_tag="JZ382_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAY2428225.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5377..8193
                     /gene="uvrA"
                     /locus_tag="JZ382_002056"
     CDS             5377..8193
                     /gene="uvrA"
                     /locus_tag="JZ382_002056"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY2428226.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         2576 a         1489 c         1772 g         2364 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
       61 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
      121 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
      181 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
      241 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
      301 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
      361 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
      421 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
      481 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
      541 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
      601 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
      661 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
      721 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
      781 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
      841 ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt attcaagaaa
      901 tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc atcttctaaa
      961 aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa ccccatttcg
     1021 tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac gttcaagaca
     1081 tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat gatgtcaggg
     1141 ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga aagcatttca
     1201 ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat tgcttttttc
     1261 tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat gttttccaaa
     1321 atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg ttgtctcacc
     1381 aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat ttcaccgtca
     1441 gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga acctgaaggg
     1501 ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt taatactgtg
     1561 ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat cttacttcac
     1621 cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca agtacatgat
     1681 cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga tcttaccagt
     1741 ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta atgagataac
     1801 gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa tgattttttg
     1861 cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc cgacaggaac
     1921 ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa gagtcaaggt
     1981 gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca acccaaagaa
     2041 aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat aaatactagc
     2101 aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca caccgattac
     2161 tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc ctttcaacaa
     2221 tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag tggaagactc
     2281 actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt attcgcctgt
     2341 tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat tttgtccttt
     2401 tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt ccaattcttg
     2461 tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat cgattgcgcc
     2521 aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag aatagccata
     2581 ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg ctccgactgt
     2641 ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga cagctaattg
     2701 gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt catctgttac
     2761 agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca aagcagcaga
     2821 gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt tttccatcaa
     2881 ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg gagcaaaagc
     2941 agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca ttgtctcgct
     3001 tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa acgcaaatgt
     3061 cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt tcataatcaa
     3121 cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca gacaagactg
     3181 taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata aaacataaca
     3241 tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt ttttagtaag
     3301 gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa tgaatgaaaa
     3361 ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt ctcgaaatac
     3421 caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt agtcggcggg
     3481 aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac gatttccaat
     3541 cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac attggctggt
     3601 cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata ttttgtttct
     3661 tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta tatcgaaaaa
     3721 gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag ttcattatta
     3781 gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt aggctcgcct
     3841 tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga tcgcaatcag
     3901 ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt tcagcgcggt
     3961 cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga tgaacgtgcc
     4021 ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa tgcactgaca
     4081 ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca ctttgtgacg
     4141 aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga acagcgttta
     4201 actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca gcgaaccaat
     4261 tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa ttattctcgg
     4321 catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt ctttcctgaa
     4381 gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag aggaatgtat
     4441 aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt gccatctgct
     4501 ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca gatcatttat
     4561 gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat ccaacaaatc
     4621 atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat gggacaaatc
     4681 gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt gtttgtaacg
     4741 acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt agggatcaaa
     4801 gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat tcgagatctt
     4861 cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg attagacgta
     4921 cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt acgtagtgaa
     4981 cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa agtaatcatg
     5041 tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc aagacgtcgt
     5101 acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat cattaaagaa
     5161 atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga agcggtacaa
     5221 gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa attagaaaaa
     5281 gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt gcgagatatg
     5341 attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa aattacgatt
     5401 catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg agataaattc
     5461 gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga tacgctgtac
     5521 gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca gtttttagga
     5581 caaatggata agccggacgt agatagcatc gacggattga gtccggcgat ttctattgat
     5641 cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga aatcaatgac
     5701 tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga tcatatcgag
     5761 attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc tgagcgcaca
     5821 aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa aaaaatattc
     5881 gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat ctatgattta
     5941 agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt catcgaccgg
     6001 atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc ggccctgcga
     6061 ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt gtttagcgaa
     6121 cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag actgttctca
     6181 ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa attagaagtc
     6241 gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc tatcgttcca
     6301 tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg tgaaagtttc
     6361 ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga gatcatcttg
     6421 aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg cgttcgcgat
     6481 gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca tgatacgaac
     6541 agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg tcagacatgt
     6601 aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac gaatatcggg
     6661 caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt aacgttatct
     6721 gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag actttcattc
     6781 ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac attatctggg
     6841 ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc tggagttttg
     6901 tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg actgattggc
     6961 tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca tgatgaagat
     7021 acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga acaaggcgga
     7081 caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc tttgacaggg
     7141 aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa aggtaatggg
     7201 aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag tgttgaattt
     7261 ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag tacattggtc
     7321 aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc taagccaggt
     7381 aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat cgatcaaagc
     7441 ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt tgatgatatc
     7501 cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa agggcgtttt
     7561 agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat tattaagatc
     7621 gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg taaacgatat
     7681 aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat tttggaaatg
     7741 acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg caaactgcaa
     7801 acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac gacattgtcc
     7861 ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc taatggaaag
     7921 aatttctata tactagatga accaacgaca ggacttcata gcgatgacat cgcccgcttg
     7981 ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat tgaacacaat
     8041 ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg tggagaaggt
     8101 ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa agaaagttat
     8161 acaggtcact atttgaaaaa aataatggta taaagaggat a
//
LOCUS       DADJYG010000113         8081 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.117, whole genome shotgun sequence.
ACCESSION   DADJYG010000113 DADJYG010000000
VERSION     DADJYG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.117"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            22..1176
                     /locus_tag="JZ382_002057"
     CDS             22..1176
                     /locus_tag="JZ382_002057"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAY2428227.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1198..1950)
                     /locus_tag="JZ382_002058"
     CDS             complement(1198..1950)
                     /locus_tag="JZ382_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2428228.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2314..4980)
                     /locus_tag="JZ382_002059"
     CDS             complement(2314..4980)
                     /locus_tag="JZ382_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAY2428229.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5321..6772
                     /gene="cls"
                     /locus_tag="JZ382_002060"
     CDS             5321..6772
                     /gene="cls"
                     /locus_tag="JZ382_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY2428230.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7101..7916)
                     /gene="thiD"
                     /locus_tag="JZ382_002061"
     CDS             complement(7101..7916)
                     /gene="thiD"
                     /locus_tag="JZ382_002061"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY2428231.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2348 a         1686 c         1451 g         2596 t
ORIGIN      
        1 cataagtaaa gaggtgttga aatgaaaata gggattgatc gtctttcctt ttttattcct
       61 aatttatatt tagatatgac agagttggca gaaagccgtg gggatgatcc tgcaaaatac
      121 catataggga ttggtcaaga ccaaatggca gtcaatcgtg caaatgaaga catcattaca
      181 ctaggagcaa atgctgccag caaaatcgta acagaaaaag accgtgagct aatcgacatg
      241 gtcatcgtcg gaacagaatc cgggattgat cattcaaaag cgagtgcggt aattatccac
      301 catttactga aaatccaatc ttttgctcgt tcttttgaag taaaagaggc ttgctacggt
      361 ggcaccgcag ctttgcacat ggcgaaagaa tatgtcaaaa atcatccaga acgaaaagta
      421 ctagtcatag caagtgatat tgctcgttac ggcttggcaa gcggtggtga agtgacgcaa
      481 ggtgtcggtg ctgttgcgat gatgatcact caaaacccgc gtattttatc gattgaagac
      541 gacagcgtat ttctgacaga agatatctat gatttctggc gtccagatta tagcgaattt
      601 cctgttgttg atggtccttt atctaattct acgtatatcg aatcattcca aaaagtttgg
      661 aatcgacata aagaattgtc gggtcgagga ctcgaagatt atcaggcgat tgctttccat
      721 attccgtata ctaagatggg aaaaaaggca ttgcaaagcg tattagacca aacagacgaa
      781 gaaaatcagg aacgtcttat ggctcgctat gaagaaagca tccgttacag ccgacgaatc
      841 ggtaatcttt acactggttc attatacctg gggctaactt ctctactgga aaattcgaaa
      901 tcactacagc caggagatcg catcggtcta ttcagctacg gttctggtgc agtaagtgaa
      961 ttttttacag gttatctgga agaaaactat caagaatatc tcttcgcaca atctcatcaa
     1021 gagatgctag attctcgaac acggatcact gttgacgaat atgaaacgat tttcagtgag
     1081 acgcttcctg aacacggaga atgtgcagaa tatacttcag atgtaccttt ttcgattaca
     1141 aagatcgaaa atgatattcg ttactacaaa atataataaa atcataaaaa gaacccttca
     1201 cgagtcgtga aaggttcttt ttttatctgc ggaccaaagg agcagtctat aggtcagctt
     1261 agtcgtacta aggatttctc gtttttcact gaatagtttg cgaatgtacc tggtcacaac
     1321 aggatctttt gcgtacgcgt catagtatag cttcatcaat tcttcagtaa ttgattcctg
     1381 tgtaaaataa gtaaaatctt ttttcttcac tttgaatcct tcatcaatta agtatgtttc
     1441 aaattgatcc attacaactg gcgatgtatt aggatcttct tctatcattc ctagttgctt
     1501 ttcgatagga taaaaaacat catcgattga ttccactact ctgattatca cgcaccattt
     1561 gcttgacttt ttcatcaatt tttctaattg aataagatgt accaatgctg gatttgctgc
     1621 tgtaaaaacc atttcatatg tctcatttga ttggataaat tcttctaacg actgtttgat
     1681 aaaggaaaca tttggcaact catttttttc tgcttctttt tttgcatttt ctagcattcc
     1741 tgaagaaaaa tcgatcaaat catagcgatt aatttctttt gctaaggaaa gaaatctgcc
     1801 agatcctcca gcaacgtctg ctgctgtttc agcaggcagg actttctccg ttttcagata
     1861 attttttaca tcttcagcaa tagttacttg cgattcttgc tggatttcat aatattcttc
     1921 agcaaattca tcccattctt tttcattcat ttgatttcct ctaatttttt gttatatgtt
     1981 ttcacttttc ggaataaatc attctcctct tttatctatt ctatctcttt tgatgaataa
     2041 taaaatgatt ggctttccgt ctatcataaa taaaaaaagg gactgtgacc aaagtcgtgt
     2101 caaagtccct ctttccgaat aaacagtgtc caatcagatg accctcagta ttagctcaaa
     2161 atgctgattt tacaacttct tccttaaacg gtgttctcct gccaactctt cggtcacaag
     2221 tcacaatatt caacaaacat gagaagctgt ttgttgaaat tgcaccttgt gactcagaat
     2281 taaacggcag gctcacaacc tcgttgattc gttttattta tttccttgta ctcgtacaat
     2341 gatcaattta gtgatttcca tcaaaacaat tgtcacaagt gcgattccca tacatgtaaa
     2401 gacttctgtc aatccaaatg ctgatgggat agagaagact tctttcagtc ctgggatcat
     2461 tgttaagcag taaagcgcgc tacatacaac tacagctagt aagacacttc tgttagagaa
     2521 gaaccctgct tggatagctg tttgtgtgtt tgaacgtgct gggaatgttt gcaatgtccg
     2581 acaccaaatc aatgttgaaa atgacatggc tacaccgaat tcaggtgaat gctgcatgcc
     2641 aagccattga gcaaagataa cagcaatccc aatcagcaca ccacgatacg ttacagaaat
     2701 caatgttttg tccgtaaaga ttcctgaatt tggatctctt ggcgggcggt tcatcgtagc
     2761 aggttcagct ttttctgtac ctaatgcaat tgctggcagt gaatcattga ctaaattgat
     2821 gaacaacagt tgtaatgctg taaatggatt ggaccagtca gcaactaagg caaagatgat
     2881 cgcaatgatc gcacctaaat ttcctgcaaa tagataagaa atagctttct tgatattatc
     2941 aaacacatta cgtcctactt ctactgcgct gacaatagaa gcaaagttat catccgtcaa
     3001 taccatcgct gctgcatctt ttgccacatc tgtacccgtt cccatagcaa ttccgatgtc
     3061 tgcttgtttc aatgctggtg cgtcatttac accatcacct gtcatagctg agatatgacc
     3121 tttattttgc caagcacgaa cgatacgaat tttattctca ggtgaaacac gtgcataaac
     3181 agtgactttt tctaaaactt cgtttagttg atcttctgtc aattcatcta gttccgtccc
     3241 ggtcaaagcc aggtttcctt cttcaaaaat cccgatttgt tttgcgatag caacagctgt
     3301 tgttttgtgg tcaccagtaa tcatgattgt tttgattcct gcacttcttg cttctgttac
     3361 cgcctgagtc acttcagcac gtggaggatc gatcatagca agcaaaccaa caaggatcaa
     3421 atcgtcttca tcttcaaatg ataaatcaaa attatcaagt ggacggtaag caaaagctag
     3481 tacacgtaat gcgcgtgttg aaaaagcttc attttgctct tggaattttt tcatcaattc
     3541 atccgtcatc ggaacgactt caccatttag caatactttt ttactgcgtt ggaagacgat
     3601 atctggtccg cctttaacaa acaagtaatt tttgttatca attgtgtgtc cagtagacat
     3661 taattttcga tctgagtcaa aaggaagttc tgcttgtctt gggtagttgt ttcggatttc
     3721 ctgataaggc tggtttaatt tttcactaaa gtcgataaac gctacttccg tagggtcgcc
     3781 caatttagta ccgtcttcac tgatcgatgc atcattcgct aaaacactga tttcgatcaa
     3841 gcgacgttcg tcagctgtcc attttgaagg gtcatttgtg aaatccccag tatcgccatt
     3901 acctaaaaag tagtcaacaa ctgtcatttt gttttgtgtc aatgtaccag tcttatctgt
     3961 acagatgata cttgtcgaac caagtgtttc aactgctgga agcttccgaa taatggcatg
     4021 acgttttgcc attttattcg ttcccaaaga taacacgatt gttacgatag actgtaatgc
     4081 ttctggaata gcagctacgg ctacggctac ggcaaacatc actgcgctga cgatatctgc
     4141 agtcatatct cctgtacctt ctccaagata aatacgaaac agttggatac ctaaaatcaa
     4201 taaagacaat gccaaaatag ccaaacttag ttttttactg aatttatcca gacctcgttg
     4261 taacggagtg acttgctcag ttgttgattc caaaaggcca gcaacttgtc cgatctccgt
     4321 attattccca gttgaagtca ctaagaattt accgcgtccg taagttacga tcgttccact
     4381 gaataccata tttgtacgat ctccgattgg taccgtactt gtgatatccg ttacttcttt
     4441 ttctgcagaa gtcgactctc ctgtcagcat accttcatct acttttaatg atcccgcatc
     4501 tagtaaacgt ccatcagcag gtacataatc gcctgcgtct aacaacacga tatctcctac
     4561 cacaagttcg tttgccggaa tcgttttttc cttaccatca cgaatcactc gagcatcggg
     4621 cgctgaaagg tttttcaacg cttctaaaga accttctgct ttttttgttt ggacaacact
     4681 gattatagaa ttcaacatca atactgcaaa aataactaag aattcgatgt agtcacccat
     4741 gaccatctga atcagtgcga caatcagcaa tacgataacc attgcatctt tgaaagtttc
     4801 taagaacaat ttccaagttg gcttcttcgg cttttctttt agctggttca agccatctct
     4861 ttctaatttt tccttcgctt gcgtggtgct caatccatcg gttgagctct gcgtttgctg
     4921 catcaacgag tcgatcgttt ctttatagac cgcttgttgt gcttgttcct tgttttccaa
     4981 atttcatccc ccattttttg atcttggccc tagtcctgtc tgaaaaaaaa gagacctaag
     5041 acaaatacgc atttgtctta ggtctcacca tttcatacaa taccagaaaa ccatttggtt
     5101 aacttactgt tgatattatc gcaacaaacg ttgccggtta ctcccctaag gagatattca
     5161 actgtactaa tatcctacct tattaaccta caaaaagcaa ctactttagc ccgatttacc
     5221 tacattttca tgaaaggagt gaaaagtata ttcaccttgg taggcttctt tcttctatca
     5281 tgctatacta atgttattgt atgtaaggag gaacattttt gtggtatcta gtattataac
     5341 cgccctttat ctattaaatg cacttattgc tttggtggct attttgatca aaccccgaga
     5401 tgtagcagcc atttgggcat ggctcttagt attgtttgcc cttcctgggg tgggctttgt
     5461 attatattta tttttcggac gtggattaac ggataagaaa aaattctatc tccgacaaag
     5521 tgacttgaaa gaattagaaa actttcagtc ttttagagga gataccattg aacattacga
     5581 tcctgacatg ggcgataaag acaaacagca atttgttgac ttcttctctt cattaaatcg
     5641 tatgccgctg acaagaatga attctgtcac tcttctcaca gacggacaag agaaattgga
     5701 ttcactgctt caagatctaa aaaaagccaa acattcgatc catatcgaat attacgcatt
     5761 tgtgacagat aatatcggcc agcaagtctt acatgtttta gaagaaaaag ccgcagaagg
     5821 cgtggaagtt cgaatattat atgatgcatt tggctctcat ggcacaaaag caaaagattt
     5881 caatcgtcta atcaaaaatg gtggacatgt ccatacattt gttacctcac aaagggcatt
     5941 acttcgtttc cgattgaatt accatgatca ccgaaaaatc gttgtgatcg atggaaagat
     6001 tagttatacc ggtggtttca atattgccaa tcaatatgta aatacaacaa aaaaattcgg
     6061 ctattggcgc gatacgcata tacggatttt cggtgccgct tcttctttgc tccagcttcg
     6121 cttcttaaca gactggaacg tctcggtacc tgaagaaaaa aaggtcggct atcatttgaa
     6181 ttatttcttt aaaaaagcag atcgagatga atctaagctt gctgatacat ccatccagct
     6241 tgtttcaagc ggaccgaata acgaaaggga acaaatcaag ctttcattta tcaaattgat
     6301 tacttctgct aaaaaacgtg tttggataca gacaccttac cttgttcctg atgaaagtgt
     6361 cattgctgct ttaaaaatcg caactgcctc tggtgtagat gtgaaaatca tgattcccaa
     6421 caaaccggat catcctttta tttatcgagc aacacaatat tatgctcggc agctgatcaa
     6481 ggaaaatgta caaatccttg tctatgagaa cggcttcctc catgcaaaaa cattgataat
     6541 ggatgatgaa atctgcatgg taggttcagc aaatcaagat attcgtagct accgattgaa
     6601 ttttgaaaca agtgctgtca tttacgatcc tgagttttta gaagaacttg ctactcagtt
     6661 caaagaagat gagacacatt gttcatccat gacaactgaa acagtcaagg aaatgtctaa
     6721 ctggctatta ttcaagcaac aaatttctcg attattttct ccaatcctat aattttttta
     6781 ttcaagtaga gaaaaatata ctaagattaa agggactgtg acaaaaatct tgtcacagtc
     6841 ccttcttccg aacaaacggt gttctcctgc caactcctcg gtcacaagtc acaatattca
     6901 acaaacatga gaagctgttt tttgaacttg ttccttgtga ctcggagctg gacggcaggc
     6961 tcacgacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata agtccaacca
     7021 agttaaaaac ataagaagct gtttttactt ggctgttctt attacttgat gcagggcaac
     7081 tttttcacaa cttctttctt ttactctaat tcaccagctg cccaatgatt gatggggcca
     7141 tatttgtgcc ctacatttat ttcttcacga attgcacggt ttacatagtc ttttgccaat
     7201 cggatcgctt gttcaactgt ttttcctttc gctaactcag ctgcaataca tgcagatagc
     7261 gaatcgccag ttccgttgat ccggtcggta ggataaaatg gtgcatttaa ccaaaatgat
     7321 tttcctgatt cgaacaatac gtagtcattg actgtttctt gctgcgcatt tttagcatga
     7381 tgtcctttga tcataacgtt ttttgcaccc atatcttgca gttcttttgc tgcttgcaca
     7441 aaatcttctt cagctgctaa tgtccgtcca gtcagctttt ctgcttcata aaaattaggt
     7501 gtcagtactt cggccaaagg gacaagcttt tctttcaagc tttgcaaagc agattcttct
     7561 agtaaaagat ttccatgttt cgttacgatt acgggatcca cgactaatgg tccaaaatca
     7621 tatttttggt aattttttac aactgtctca atcaactttg aatctgctag catacctgtt
     7681 ttagctgcac gtatttgaaa gtccttcgca atcagttcaa attctttgtc gataaaatct
     7741 gttggcaatg tcacacttgc tccgattccg taagaattac ctgcaacaca tgcggtcatg
     7801 acagaaacac cgtaaacatt tcttgtaaag aatgtatgca tatctgcttg catccctgcg
     7861 cttccatcgc tatctgatcc tgcgatcgtc attgcttgta catactgatc ttccatcaaa
     7921 tcgattcctc catttttata aaaagatttt tcataatact gttactgttt ctatcatatg
     7981 tttttcaaag tatccctcag taaatatacc actacctttt cttggtaatc aaatttattt
     8041 gaaaaaacat ttgcaataaa tcagaaaata gtggtcgttt a
//
LOCUS       DADJYG010000114         8066 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.118, whole genome shotgun sequence.
ACCESSION   DADJYG010000114 DADJYG010000000
VERSION     DADJYG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.118"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            155..655
                     /locus_tag="JZ382_002062"
     CDS             155..655
                     /locus_tag="JZ382_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428232.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(845..1189)
                     /locus_tag="JZ382_002063"
     CDS             complement(845..1189)
                     /locus_tag="JZ382_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAY2428233.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1343..2263)
                     /locus_tag="JZ382_002064"
     CDS             complement(1343..2263)
                     /locus_tag="JZ382_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2428234.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2450..2941
                     /locus_tag="JZ382_002065"
     CDS             2450..2941
                     /locus_tag="JZ382_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAY2428235.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2965..5340)
                     /locus_tag="JZ382_002066"
     CDS             complement(2965..5340)
                     /locus_tag="JZ382_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY2428236.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5666..6292
                     /locus_tag="JZ382_002067"
     CDS             5666..6292
                     /locus_tag="JZ382_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAY2428237.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6315..7019
                     /locus_tag="JZ382_002068"
     CDS             6315..7019
                     /locus_tag="JZ382_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY2428238.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7211..8044
                     /locus_tag="JZ382_002069"
     CDS             7211..8044
                     /locus_tag="JZ382_002069"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY2428239.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2480 a         1529 c         1454 g         2603 t
ORIGIN      
        1 acttcaaata atttttttat tgtaattcat atttgtattg ttcgatgatt cttaatacga
       61 tatgacacaa taaataaaaa taggcatggc tatttacaac aatagtgcta tagtagaaaa
      121 caaatgaata aggttactta aagaggtgaa tacgatgaaa ctgatcaaac tatgggaagc
      181 agatttaaag aaagcttatc agctacaaac tagctttgga ccaaatgaga gtggatttat
      241 caacaatgct tatggctata catttgctga atttttagat tatgtaaaaa aatgcgaaaa
      301 aacgtcttta ggaatcgatc ttcctgagag ttacgttccc gcaaccgtct ttattttaga
      361 aagtgatgaa catgagtatg taggaatttt taatttgcgc cataaactga acgaaacatt
      421 aagaaacggc gctggccata ttggttacgg catctcccct aattatcgta aaaaaggata
      481 tgccacacaa ggattagcat tagtactaca agaggcaaaa aaaatcggca ttgatgaggc
      541 acttttgtct gtaaataaaa aaaatcttgg cagtcttaaa gcacaacaaa aaaatggggc
      601 tgcgatttat cgtgaagatg agaaagaata ctatacgaaa atcaaactga tgtaaaaaag
      661 aaggattcca aaaggaaagg gatttgaatg tcctttatca atagaacaca tttatttaat
      721 taacttataa tatagcagac aaaattttat aaacaaagaa accttttatc tcttttcttc
      781 tagaaaggat aaaaggcttt tttgtttttc ctatttgctt ctcatagcta gtcagccaag
      841 atttctatcg tatccccaac gcaatccgtg cataacggct catcttatct gtcgtccatg
      901 ctggatacca gactagtttg acttctgctt tcgttacttc tggcacttct tttaatgcgt
      961 catggataga ttctgttaaa atatctgcta atggacagcc catagttgtt agagtcatct
     1021 taatgactgt ttctccattt tcaggattga attctatatc ataaatcaag cctaaattga
     1081 cgatgtcgat tcctaattca ggatcaatca ccatttccaa ggcagcaaga atacgttctt
     1141 tgattagttc tatttcttgt ccagaccgtt cttggtttgc ttcgctcatc gtacgacact
     1201 ccttaacacg atttttattt atcatactct ttttttagct gcttgtaaat gagaatttag
     1261 aagtaagatt ctcgtttaaa tttataaaaa tgttcttatt agatgtaata gattaaattt
     1321 attctaaaat aaacctgatt cctcattttt tttgtgaacg attttttatt tctggcaata
     1381 cgccagttgt tcgatacatt ttttgttcgt tttcatttgc aaaccggcca aatactgaat
     1441 gagttggtaa aatattgata aatgcatcgt cgtcgatttc atgaacgatc tgatcaagat
     1501 catacatttc gtagcgggtg atgaccatca tgatcatgtt cccttgtttt tttgaatagc
     1561 caccgacaga cggcagtaat gtcattccac gtaccatccg ttttgctagt tcttctgtta
     1621 cctcttctgg cttgacggta acgatcattg ctgttacttt ttgatgactc gtgtggatgg
     1681 catcaattac ttgagtcatt gcataaatgg aaataatcgt atacaaggca ctttcccagt
     1741 taaagaagaa gcctgcgatg actacaatga taccatttag aatcagcatg tagtttccga
     1801 ctgtttttcc agttgtttgt gaaagtacaa gagagatgat atccatccca ccagttgtaa
     1861 agcccatttt taaagataat cctacgccaa gacctaataa aacaccgcct actagggcat
     1921 tcatcaaaat attatcagtg atctgtccga ctggaagcag catcgtcatc accgatacac
     1981 atacaacatt cacaaaacta aggatcgttg ctcgttttcc tactttgaga aaccctaata
     2041 caaacacagg gatattcaaa agaaaaataa atgttcctgt gtctatcttg atatgaaacc
     2101 attcatataa aagcgaggaa atgatttgag cgatcccatt catccctgca gaaaagacat
     2161 tggctggaat caaaaaataa tttaaagcga ccgcagcggt caatccggtt atcaaaataa
     2221 ccactatttt ctttaatctt tcatttttat gatacgcagt cataatttta ctcacaagca
     2281 cctctcccct actttggtat tccaaatgta agaataacag tttatctatt aaaataaaat
     2341 acttcgaact caaatgagaa gaattttagc aaacaggtaa gaatctgccc taatcctatg
     2401 ataagataga tgtatggtaa atatttatat gtatatcagg agggttccta tggcaaaaga
     2461 tgctagcttc gacattgtat cagaagtcaa tacagaagaa gtaaaaaatg cgattcagca
     2521 aacaacaaaa gaattgtcca atcgctttga cttcaaggat tctacagtag aaatcaaatt
     2581 agagaacagc aatctagttg tattgagcga tgatgatttc aaaatcgaac agatcaaaga
     2641 tgtattattt ggtaagctga tcaaaagaaa tgtgccaatc aaaaatatcc atttttccga
     2701 atcagaacac gctctaggtg gcaaagctcg acaaactgcc gatttggtca acggaatcga
     2761 tcgtgaaaac gcgaagaaaa tcactacagc tatcaaaaat gaaaaactga aagtaaaagc
     2821 acaaatccaa gaagatcaga ttagagtgac aggtaagaac cgtgatgatc tacaagctgt
     2881 gattgcttta ttacgtaaac ttgacttacc aatcgagttg caattcacta actatcgtta
     2941 atcaaaaaac gaggctgacg tctgtcattc ctcgtttttt tgatttactg cttcttttga
     3001 taaccaaaaa cgctgtattc cttcttccaa ttccccaaca cttcgtactt ctcttgctgt
     3061 ttgccaatgt tgtgggaata aacgtcgata agaaaaacta gaaaatcgtt gatctgccaa
     3121 tacaacgatt ccttgatccg ccatgccacg aatcactcga ccaccagctt gcaagacttt
     3181 gttcattcca ggaagctgat aagcgtaagc aaatccaagg tgttccttct catcataata
     3241 tgatttgatc agttcctgct catgattcat ctgcggcaaa cctacaccta cgatcatact
     3301 gccgatcaat cggtcccctc tcaaatcgat tccttctgaa aaaatcccgc caagtacgca
     3361 aaatccaatc aatgtttcag cgggatttgt ttgaaatttt gttaaaaatc gttcgcgttc
     3421 ttcctcattc atgtccgttt cttggattag cacattgctg tcgggatatt gctgtctgaa
     3481 tgcttccacc gcaagatcta aatattgata agaaggaaag aaaacgaaat aattcccgac
     3541 ctttccatca gcaaaagcat gaatcgtctc tactaatttt gccagactgt gttccctttt
     3601 tcgataagtc gtctctaggt aatttgcgat gaaaacttgc tggtttcctt ctgggaaagg
     3661 actcggcagt ttgtaagcca gacttttttg tccccccagt gtttcctgat aataagagag
     3721 tggtgaaaaa ctagcagaaa aaagaatact gctttttcct ttatccaacg actgctcaag
     3781 aaacaagctt ggatcaatac aaaactcttt tactatcaga tcgcgatacg ttttttcgac
     3841 tgttgtttcg tagtgatcat cataaaattc actgaccttc aaaaaatgaa gcaaatcaaa
     3901 ataataagga agcaattgtt cttgtgatgg gtgctcagga aattctgcca gccattcttt
     3961 aattttttcg cttaattgat aacctgcctt tactaaagat tcagccggtg ctttttgatg
     4021 atgataatcc caatgatctt cttttgctat ttctcggatc gaatcaaatt cttttaatag
     4081 tttgttgaaa cggcgatgaa gttttttgaa ttcctttggt atagcttcct tacttcgttt
     4141 cgttttttca taagatagtt ctgccgaata catctctcgg gaacgattca ccaaattatg
     4201 tgcttcgtca atcaaaaaca gatagtcctc gtttttttca tcttcaaaaa aacgtcttaa
     4261 ataaacagtt ggatcaaata aataattgta atccccaatg attacgtcac accagatact
     4321 gacatctaat gatagttcga aaggacaaag tgtatgcttt cttgcataag tttcgatgac
     4381 ttcacgagtg atctgattct catgattcaa aagatcccat aaaccttcat taatccgatt
     4441 gtaataacca ttggcatatg ggcactgatc tggattacag gtcgtctcat ctaaaaagca
     4501 gatcttgtct ttcgccgtca aagtgacact ttttacttca gctcctgtat cttttaatgc
     4561 tgtcatagca tcttcagcta cttgtctagt tattgtttta gccgttagat aaaagatctt
     4621 ttctccctct tcttccccta cagctttcaa agcggggaat aacgtagaga ttgtttttcc
     4681 tgttccggta ggtgcttcta caaacaattt ctgtttcgta cggatcgttt tataagcagc
     4741 taccgctagt tcacgctgac ctttcctata ctcatcatgg gggaatttca aagccatcaa
     4801 agaggcattg cgtacagtgc gccagttttc ttgaaaaaca agccattctt tataatcttc
     4861 agtcagcttt ttaaaaaaag tttctaactg ctctcttgtc tgatgttcaa cctttctcgt
     4921 gatcacttct tctgccgtct ggaagtaagt caattgcaga tttatttctt gtaaatcatg
     4981 ttcttgacta tagatatagg catacaccct tgcttgatga aagaaaagtt ccacttgttc
     5041 tggttctaaa tcttcaaatc gtggttcgga ggttttgatt tcatctatat aataaatacc
     5101 gtctcttttg aaaataccgt ctgcacggcc ttcaacgatc aggtaatatt catctaattg
     5161 aactgttgtt tttaaaaaga cttctttttc atagttttct cccgcatctt tttggagctt
     5221 gcggtggatt ctcgctcctt ctaatgcagt atggttactt tttttacgat tgtcgatact
     5281 tccacgtcgc aaaataaatt ctactaagtg ccgcacagat atttttgact gtttgatcat
     5341 ttccaccacc tctttctctc taagattacc tgaaaaaacc tagtttagca aaaagagctc
     5401 tctttccaaa cataaaaaaa caccctgctg attaggcagg gatataggat atagaatttg
     5461 gatttttgga tgaatctaat gtaacaagga catattaaaa tgctatgaaa acttactttc
     5521 cttctcttta gaatatccaa atacttttca tatagtctga taattcagat aagtaaagag
     5581 aaataagtac ctgtttttgt ttcctagcaa tgaatgcgtt atactaatgg atgaaagaag
     5641 ttttggctat caaggaggaa tgcacatgca tatcgaaaga ttaaaaacag gaatgattga
     5701 agaaaactgt tatttggtct acaacgaaga agcacttttg atcattgacc ctggagaaga
     5761 tgcggaacga atcaaacaag aaatcaaaaa aacaaaccaa cagccggtag cgattttgct
     5821 tacccatact cactacgatc atatcggtgc agtggaacct ttacgcgcgt attatcaaat
     5881 cccagtttat gtcagcccac tagagcaaaa atggctaggt gatccgattt tgaatctttc
     5941 tggactcggt agacatgatg atattgctga tgttattgtc tcgcctgcag aatatgaatt
     6001 tgaaatgaaa acttatactt taggaaacat gtcttttcgc gtcgtcccta ccccagggca
     6061 ttctatagga agcgtcagct ttatttttga tgattttgtc gtttctggcg acgccctgtt
     6121 tagaggaagt atcggtagaa ccgatctgta tacagggaat ttagaacaat tacttcatag
     6181 tattcggacc cagctgtttg tattacctga tgaatttgca gtctatccag gacatggcga
     6241 tgcaacgacg attgaacagg aaaaaaggac aaatcctttt ttcaatcgtt aaaacgactt
     6301 aggaggagaa taaaatgaca gaaactacac tttatatcgt acgacacgga aaaacgatgt
     6361 tcaatacaat tgaacgagta caaggctggt gtgatacacc cctcacaaag caaggacaag
     6421 aaggcattca ttatttaggt cgcgggttaa aaaatgtcga cttttcattt gcttattcta
     6481 gtgacagtgg tcgtgcaatc gaaactgccc gtattattct aggagaacat tctaagggaa
     6541 aagaaattcc ttattttatt gaaaaaagga ttagagaatg gtgtttcggg tctcttgaag
     6601 gtggatatga catggaaatg tggggtgtga tccctcgtgt attgaatttt aaaacatatg
     6661 atgaaatgtt taccacagaa gtttcttttg aacaaattgc taatgctgta tacaacttag
     6721 ataccgctgg gtgggcggaa acttatgaaa cattgcgaga ccgtgtatgg acaggatttg
     6781 aagatatcgc ccatcactgc gaaaaaaatg gtggtggaaa tgttttagtc gtatctcatg
     6841 gtttgacgat cgcctttttg cttaatctga tcgatcctaa acagcctgtt cgtgctgggc
     6901 ttttaaatgg cagcgtaaca aaggtcactt atgcaaacgg tcgattttct atccacggca
     6961 tcaatgatac gtcttatatc gataacggga aaacacatag tcgcgactcg tttctataat
     7021 ggggatattt taatttttac cagagaatca taaaaggcaa agtctgaacg agggctttgt
     7081 cttctttttt ctcttcgtgt acttatctgc tttatttctc aagtttttct taatattaaa
     7141 aaacagattg ggtttctcaa aaaataacgg tatactataa gatgtatctt aattgggaag
     7201 gaaggaattt atgttttttg caaatattat caaagcagtg attctcggta ttattgaagg
     7261 aataacggaa tggctgccca tcagtagtac tggtcatttg atcctagcag atgaattcat
     7321 caaactggat gccagttcac aatttatgtc gatgttcaat gtcgttatcc aattaggggc
     7381 gatcttggct gtcgtcgttc tttattttca caagttaaac ccattttctc caagtaaatc
     7441 aacggtagaa aaaaaagata cgtggacact ttggtttaaa gtgatcgttg cttgcatacc
     7501 agccatcatc attggtttgc ctttggatga ttggttagaa gcaaatttcc acaagttctt
     7561 accagttgcc attgttttga tcgtttacgg tattgccttt attattgtcg aaaaacgcaa
     7621 caaaaacaaa acacctcgtt ggacttctct gaatgacttt acgtaccaag cagctcttct
     7681 tgttgggtta ttccaagtct tggcacttgt tcccggaaca tcccgttctg gcgcaacgat
     7741 cctaggatct atcttgattg gtgcttcgcg ttttgtcgct acagagtttt catttttctt
     7801 agggattcct gtcatgtttg gcgcaagcgg cttgaagatc gtcaaatatt tagctgaagg
     7861 gaattcattc aaaatggaag aaactgttgt tcttcttgta ggaacattcg tatcttttgt
     7921 ggtttccgtc ttagctatca aattcttact aagctatttg aagaaaaacg actttaccgc
     7981 ttttggctgg tatcgtatta tccttggtat cattttgatt ggttattggt ttattgcaat
     8041 gtagaaaaaa ccagaacact tctctt
//
LOCUS       DADJYG010000115         7974 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.119, whole genome shotgun sequence.
ACCESSION   DADJYG010000115 DADJYG010000000
VERSION     DADJYG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.119"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            264..2030
                     /locus_tag="JZ382_002070"
     CDS             264..2030
                     /locus_tag="JZ382_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAY2428240.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2132..3391
                     /locus_tag="JZ382_002071"
     CDS             2132..3391
                     /locus_tag="JZ382_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY2428241.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3538..4839
                     /locus_tag="JZ382_002072"
     CDS             3538..4839
                     /locus_tag="JZ382_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2428242.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4842..5693
                     /locus_tag="JZ382_002073"
     CDS             4842..5693
                     /locus_tag="JZ382_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2428243.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5706..6521
                     /locus_tag="JZ382_002074"
     CDS             5706..6521
                     /locus_tag="JZ382_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAY2428244.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6672..7838
                     /locus_tag="JZ382_002075"
     CDS             6672..7838
                     /locus_tag="JZ382_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2428245.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2603 a         1376 c         1587 g         2408 t
ORIGIN      
        1 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
       61 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
      121 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
      181 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
      241 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
      301 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
      361 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
      421 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
      481 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
      541 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
      601 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
      661 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
      721 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
      781 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
      841 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
      901 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
      961 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     1021 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     1081 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     1141 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     1201 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     1261 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     1321 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     1381 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
     1441 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     1501 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     1561 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     1621 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     1681 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     1741 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     1801 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
     1861 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     1921 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     1981 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     2041 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     2101 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     2161 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     2221 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     2281 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     2341 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     2401 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     2461 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     2521 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     2581 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     2641 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     2701 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     2761 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     2821 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     2881 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     2941 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     3001 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     3061 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     3121 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     3181 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     3241 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     3301 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     3361 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     3421 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     3481 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     3541 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     3601 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     3661 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     3721 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     3781 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     3841 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     3901 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     3961 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     4021 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     4081 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     4141 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     4201 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     4261 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     4321 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     4381 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     4441 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     4501 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     4561 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     4621 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
     4681 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     4741 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     4801 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
     4861 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
     4921 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
     4981 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
     5041 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
     5101 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
     5161 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
     5221 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
     5281 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
     5341 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
     5401 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
     5461 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
     5521 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
     5581 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
     5641 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
     5701 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
     5761 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
     5821 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
     5881 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
     5941 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
     6001 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
     6061 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
     6121 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
     6181 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
     6241 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
     6301 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
     6361 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
     6421 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
     6481 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
     6541 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
     6601 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
     6661 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
     6721 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
     6781 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
     6841 cttctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
     6901 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
     6961 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
     7021 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
     7081 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
     7141 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
     7201 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
     7261 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
     7321 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
     7381 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
     7441 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
     7501 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
     7561 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
     7621 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
     7681 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
     7741 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
     7801 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
     7861 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
     7921 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaa
//
LOCUS       DADJYG010000116         7930 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.120, whole genome shotgun sequence.
ACCESSION   DADJYG010000116 DADJYG010000000
VERSION     DADJYG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.120"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..817)
                     /locus_tag="JZ382_002076"
     CDS             complement(<1..817)
                     /locus_tag="JZ382_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2428246.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTRE"
     gene            complement(1041..2981)
                     /gene="thrS"
                     /locus_tag="JZ382_002077"
     CDS             complement(1041..2981)
                     /gene="thrS"
                     /locus_tag="JZ382_002077"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY2428247.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3318..3497)
                     /locus_tag="JZ382_002078"
     CDS             complement(3318..3497)
                     /locus_tag="JZ382_002078"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAY2428248.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3544..5367)
                     /gene="cadA"
                     /locus_tag="JZ382_002079"
     CDS             complement(3544..5367)
                     /gene="cadA"
                     /locus_tag="JZ382_002079"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAY2428249.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5645..7924
                     /locus_tag="JZ382_002080"
     CDS             5645..7924
                     /locus_tag="JZ382_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2428250.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2317 a         1696 c         1440 g         2477 t
ORIGIN      
        1 cttctcgtgt ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa
       61 aaatgatatc gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg
      121 tgatttcttt tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat
      181 ttccaaattg cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg
      241 cgactacatc tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat
      301 tgctgacaag ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga
      361 cacgagacca tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata
      421 aaaaaacagt ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta
      481 gaatatctac tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag
      541 agtaaaagga ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt
      601 ctgttgaaat cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac
      661 gtacaactgt cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag
      721 ggaccttctc aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat
      781 cttccatcat actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga
      841 tagtctatgt aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac
      901 aaaaaaacaa tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc
      961 atctgtgtct ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt
     1021 ttttacagga aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata
     1081 gcatcaataa agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg
     1141 ttcactgttg catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt
     1201 gtttgagagg cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc
     1261 aaccccttca tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca
     1321 gaaacaggga tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat
     1381 acttctgtta aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact
     1441 actggacgat gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc
     1501 aataagaagt ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact
     1561 tgaacatcta gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt
     1621 tctaattcat ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca
     1681 tcaaaatatt tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta
     1741 atattgaagt cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg
     1801 atttgatcag gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt
     1861 aaacctgaca aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga
     1921 attggtaatt cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag
     1981 ttcattgggc gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct
     2041 tcttgataat gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt
     2101 gtatatacat gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg
     2161 cgtcggattg ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa
     2221 accatgaaca agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca
     2281 cggagacgga tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt
     2341 tgcatcattt gattgtttga gtttccacgc caataagcac ctgccactga taataattta
     2401 aatacttgta tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg
     2461 ccttgttgat aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg
     2521 tacggatcac ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc
     2581 ggcagatttt ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca
     2641 gttacttggt tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca
     2701 acgccaaagt ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct
     2761 gaatgacgga ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca
     2821 tcttcctcga ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct
     2881 ttttttgcta agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca
     2941 aattctttga ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa
     3001 tgttttgatt gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa
     3061 gagatactag gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc
     3121 aagcgttgta acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt
     3181 tggttttctc ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat
     3241 tcagttggct ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat
     3301 caagtgggaa actaaactta ttttttccat tccccattta caccgtatgt tccttttttc
     3361 atctcattga tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct
     3421 tccgtgatat ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg
     3481 acatgtacga atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt
     3541 ctatcatact atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc
     3601 tctcaaggca ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa
     3661 tgcggggatt agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat
     3721 cagtacagac tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac
     3781 acgcaagaga tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc
     3841 atgagccccc attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc
     3901 gtctcctacc atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt
     3961 atcttgaggc aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg
     4021 agcaatggca gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag
     4081 ctccttgatt gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc
     4141 aaaagatcga tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga
     4201 aacaaaagag gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg
     4261 accggtcact tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac
     4321 taacgaacga gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag
     4381 ctcttctttt tttattccat tgactggctg aatcgcctct acttccaata tccctttggt
     4441 aagcgttcct gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt
     4501 tccagttttt acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat
     4561 gggcgcagct agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa
     4621 agggtctttt gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg
     4681 gactgcataa cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac
     4741 tagtttaata atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat
     4801 cgatccgtct ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg
     4861 gcgggactca cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc
     4921 atctgcgggt acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc
     4981 gattactata tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc
     5041 taacaaagac tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc
     5101 gcccgtaagc atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc
     5161 aaggattgcg gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat
     5221 catttccaaa aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc
     5281 aagggccggc tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt
     5341 aagtaaaaat tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag
     5401 gttagtaata aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt
     5461 ttaaaacaat aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca
     5521 gttctacaaa caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac
     5581 cttttttcga aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca
     5641 tgcgatgaat gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca
     5701 gatcgaacaa aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc
     5761 gacaaaagac acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc
     5821 tgctgttgaa tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc
     5881 acagcaaaaa cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat
     5941 cgactttaaa gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga
     6001 tctggcagaa gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga
     6061 aggtgagatt ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca
     6121 gttgaaacga caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga
     6181 agtaatcaat gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa
     6241 aaacatcgtc tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa
     6301 agtaatgctg attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat
     6361 tgcttttctg ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc
     6421 acccaaccat ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg
     6481 cgtgcctaca cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact
     6541 agatgaacag caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat
     6601 gaaaagcgga ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta
     6661 cggtcccttg ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat
     6721 tcgccaatgg tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt
     6781 acaaacgaag ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg
     6841 ggtcaaagat ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga
     6901 atatacagaa caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa
     6961 aagattccgt cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca
     7021 atatctccac tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga
     7081 gaccaggtgg caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg
     7141 tcaagaggat gctgtactat tctttttact gatgcgccga acatatccag tgacggttac
     7201 acacaaagct cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc
     7261 attgctgcaa gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa
     7321 tgtctttgga aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt
     7381 cactcgtttc caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa
     7441 tcattttatt gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt
     7501 gctcatccaa ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac
     7561 gactcaggga aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg
     7621 cgaagctctt tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa
     7681 tgagagtgac tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct
     7741 ggagtttgac cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga
     7801 caaattgatc ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc
     7861 caataatgaa agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact
     7921 gtaagtaaaa
//
LOCUS       DADJYG010000117         7779 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.121, whole genome shotgun sequence.
ACCESSION   DADJYG010000117 DADJYG010000000
VERSION     DADJYG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.121"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..718
                     /locus_tag="JZ382_002081"
     CDS             17..718
                     /locus_tag="JZ382_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAY2428251.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="JZ382_002082"
     CDS             complement(1327..2343)
                     /gene="tsaD"
                     /locus_tag="JZ382_002082"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY2428252.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="JZ382_002083"
     CDS             complement(2340..2804)
                     /gene="rimI"
                     /locus_tag="JZ382_002083"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY2428253.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="JZ382_002084"
     CDS             complement(2811..3353)
                     /gene="rimI"
                     /locus_tag="JZ382_002084"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY2428254.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3337..4161)
                     /gene="tsaB"
                     /locus_tag="JZ382_002085"
     CDS             complement(3337..4161)
                     /gene="tsaB"
                     /locus_tag="JZ382_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY2428255.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4250..5230)
                     /locus_tag="JZ382_002086"
     CDS             complement(4250..5230)
                     /locus_tag="JZ382_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY2428256.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5254..6738)
                     /gene="galT"
                     /locus_tag="JZ382_002087"
     CDS             complement(5254..6738)
                     /gene="galT"
                     /locus_tag="JZ382_002087"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAY2428257.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6750..7739)
                     /gene="galE"
                     /locus_tag="JZ382_002088"
     CDS             complement(6750..7739)
                     /gene="galE"
                     /locus_tag="JZ382_002088"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY2428258.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2162 a         1688 c         1386 g         2543 t
ORIGIN      
        1 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
       61 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
      121 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
      181 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
      241 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
      301 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
      361 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
      421 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
      481 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
      541 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
      601 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta
      661 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
      721 cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta
      781 aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt
      841 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa
      901 gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca
      961 gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca
     1021 ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt
     1081 cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg
     1141 aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact
     1201 cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa
     1261 catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca
     1321 acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt
     1381 tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag
     1441 tcgaagaggg ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa
     1501 tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg
     1561 ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa
     1621 gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa
     1681 attgataaat gcacttttta atccgctaaa actgaaatca taattatctt catggatcat
     1741 cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc
     1801 gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc
     1861 atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt
     1921 atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc
     1981 tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc
     2041 aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt
     2101 gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa
     2161 gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa
     2221 acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct
     2281 aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt
     2341 catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg
     2401 cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt
     2461 gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc
     2521 gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca
     2581 gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc
     2641 tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac
     2701 tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat
     2761 tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt
     2821 tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg
     2881 atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata
     2941 cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca
     3001 ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata
     3061 tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag
     3121 atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc
     3181 catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct
     3241 gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa
     3301 atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc
     3361 gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg
     3421 aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt
     3481 tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc
     3541 ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag
     3601 cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc
     3661 taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa
     3721 catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga
     3781 tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat
     3841 acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg
     3901 acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt
     3961 gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc
     4021 caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat
     4081 gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc
     4141 aaaactagta caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa
     4201 cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg
     4261 attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat
     4321 tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta
     4381 cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag
     4441 aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta
     4501 atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct
     4561 tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt
     4621 agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat
     4681 ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga
     4741 caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc
     4801 cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta
     4861 ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa
     4921 tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc
     4981 ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga
     5041 tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc
     5101 gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat
     5161 agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa
     5221 ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa
     5281 cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa
     5341 aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg
     5401 ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc
     5461 taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag
     5521 ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg
     5581 atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa
     5641 ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc
     5701 tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac
     5761 tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat
     5821 cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc
     5881 tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag
     5941 aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac
     6001 ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg
     6061 ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca
     6121 gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa
     6181 gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac
     6241 cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt
     6301 gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct
     6361 tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg
     6421 gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa
     6481 atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt
     6541 ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct
     6601 tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata
     6661 taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat
     6721 cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca
     6781 tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc
     6841 agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt
     6901 ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc
     6961 ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc
     7021 aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg
     7081 atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga
     7141 ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg
     7201 atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg
     7261 ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac
     7321 ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa
     7381 gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt
     7441 aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct
     7501 cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg
     7561 gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact
     7621 gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc cttttgaact
     7681 agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata
     7741 aaaaacactc ctttgattag taaaattgtg tttgttgat
//
LOCUS       DADJYG010000118         7774 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.122, whole genome shotgun sequence.
ACCESSION   DADJYG010000118 DADJYG010000000
VERSION     DADJYG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.122"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..1386)
                     /locus_tag="JZ382_002089"
     CDS             complement(22..1386)
                     /locus_tag="JZ382_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY2428259.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1383..2942)
                     /locus_tag="JZ382_002090"
     CDS             complement(1383..2942)
                     /locus_tag="JZ382_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428260.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2998..5478)
                     /locus_tag="JZ382_002091"
     CDS             complement(2998..5478)
                     /locus_tag="JZ382_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428261.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5462..5797)
                     /locus_tag="JZ382_002092"
     CDS             complement(5462..5797)
                     /locus_tag="JZ382_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428262.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5811..6845)
                     /locus_tag="JZ382_002093"
     CDS             complement(5811..6845)
                     /locus_tag="JZ382_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAY2428263.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6935..7615)
                     /locus_tag="JZ382_002094"
     CDS             complement(6935..7615)
                     /locus_tag="JZ382_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAY2428264.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
BASE COUNT         2231 a         1556 c         1414 g         2573 t
ORIGIN      
        1 aataaaagac cgtgatattt ctcacagtct tttatctact ctattgctat ttgctctctt
       61 agctagtact ttaaaagcta actcattgag gtagtgaaaa cgagaggtgt gttcagacag
      121 aaatccaaaa tcctcaactg ctagctgtgg aatcgtagtt ttgtgtttca cgatatccgc
      181 aagcaggttc aaatctgata ataagaattc ttggcttatc agctgtccgc caattatttc
      241 taatgtatcc ggatggtaga tcagctttgc atttactgtt ttgttttctt catcgtattg
      301 tggaaataga tcaaattctc ctgaacacga accggtattt tgttccaaga atggcgcttc
      361 atcttctgtc agccctacag ttcctaaaaa gtagtctata atgactgctg ctgttatctt
      421 ttgtctgagg tcgtaagcgg ttggctgacc actcaaagtt agcgctactg ctcttgccat
      481 atgtacagct cgagtaacta aaggtaaaaa gagatgccgt ttatttttgt caaaactgac
      541 tgggacgata tctccaatcg catagatgtc cggatcactt gtctgcagat attcattgac
      601 tatcacagtc tcatcctcat acaaagccac tttttctttt aacaatgtcg tattaggacg
      661 agtttgggca gaaaaaatga ccatatctgc ctttattgct tgagatagaa gttgtacgga
      721 tactactttc tcttctgatt ttttccaatc gatcaagaat tcatttggat aaaaattgat
      781 ttgcttagtt tgcagctgat tttgaaaact gttgatcata tccttatcga aatatcttga
      841 taaaacattc ggcatataat caactagatg aataatgtat cctttatttc tcaaagcatc
      901 tgctaattcc aatccaatat aaccagcacc gaagatcact acttcttttg cttcttttaa
      961 agcggcaagt acttgcttag attgcactag atttttatag gtgtagacat tttcagcttc
     1021 aatttgttta tacaaggtga caggagagga acctgttgct acgattaatt tatcataggt
     1081 tatggtctcg ttttttcctt ctttggatgc gaggatgagc tgatgtttgt ctggatcaag
     1141 ttcagttact ctccattcgg tgatcagttt tgccccactt tcttgaagag tccgcatgtt
     1201 atgggcaact tcagctaaac tacgtatctt tccttttaaa taaaggttga ttccatttga
     1261 aacatagcct agtccatctt tatgatcttc atcgatcagt aaaacttctg ctttcggttg
     1321 aagttttctt agatttaggg cagctgtgat gcctgcatgg gaagcaccaa tgatcacgat
     1381 tttcatgttt tattcatcct taaattcaat ttttctaaat aacattcttt taaaaattgg
     1441 ttcaaatagg ggaaatcata attatattcg ttgctagtga atacaaaaac tttagcggac
     1501 ttctgtgacg ggtactcttt caattggcta gtcaaaatga tatcatactg cttttctttc
     1561 tcgtattcga ctaaatcata tttgatcgta ttcagcaatt gttcctggat gatttctttc
     1621 acgatatccg ccattagcct ttcataagga agatcataag caatttgcag acgtaattct
     1681 aactgcttga tcagcgtccg caaaatagat gcatagcgag caaataattc aaagcgggca
     1741 ttttcctcgt ctacttcagt aaaatcaaca atagcaaatg tttgtctgac caatctttca
     1801 cataaaacca ggaattgttt atcttctact tcagttaacc gctgttttaa tccttcttgg
     1861 ccaaaaatat tcatccatcc tctaaaatag gcaaaatgag cgtgattttg catcactgtc
     1921 aacgcaatct tttgcgaaat ctcttctgga aaaacataaa aagagaaata ttcttctatc
     1981 gattgtttaa acgttcgatt caattgagct actacgtcgt ctttgtattt ttcgcttatc
     2041 aaaaactcct ggacatgcga gttatgtgta tctaatgtaa agttactgat catgaaaaga
     2101 taaaaaacca acgtttcatt ttgattccaa ggaaaggcat attgaaagaa gtaacgggtc
     2161 aaaaatgtat ggaggtccaa taaaaattgt gtcgtttgaa acaatccaac aggaagattg
     2221 ctataatctg gtttggattt tttcgtacag cgcattcgct tcttcataat ggaaagccat
     2281 atttcaaatt tcaatcgtcc ttcttctgta atagtaaaac taaactcttt ttgcagcaga
     2341 tctaaaaaag tgtttcctga taaatcactg tattcttttt tgttttcaag ataaggacgt
     2401 ccaaaccaaa ataattggaa ataaaaataa caaatttgac tttctggacc ggtcagccgc
     2461 ccgcttttta ttttcagatg aaactcattt aaagaatcat ttaattcctt cactctgcga
     2521 gacaaagtag ccgaactgat attaagttcc atgacaagtt tctgccgatc aaatgagcca
     2581 ttttccaaca gataaagaag tatctgatat tttgtagaat agcgcaagta gcaatgataa
     2641 taaacatcga ctgctgctat atttccatgt ctgattaata aaatattccc ttcttggata
     2701 acaatttcca attgtttgat attttccaag ttagcttgta aataaaacag atcttctttc
     2761 aaggtagccc gatcaactga taagcgggta agtacgttat taacggaaat ctctcctttt
     2821 tcctctaata aaagttggaa aagtttgatt tgccgttgtt catttttttc aagcaaactg
     2881 aaaagttcca agtccactcc tccaagcatt tctgatttta acagcagaag tttccttctc
     2941 atccttgaaa aagaggatct gtgacagtta tcacagatcc atacaaattc taatcactta
     3001 gctggttggc ccagctgtca gttcccatgt gaccatgcct tgatacgttg cgccaggttg
     3061 agcattcgta ctgctattta cctctaagag taaaccatcg gtatcgggaa agtcaacgag
     3121 aacctctcct ttagctgttc catcactctc ataaatcaac gtgttttgac cttgctgaat
     3181 caattgtttt gatccagaat gattgaagta taattttgtt ccgcttaatt cttgaccttg
     3241 atcattctta aataaagaag tcaatgtccc tgtcagttgc caaggagacg tgcttggaag
     3301 ccgagtatcc cgcacatcaa aagaaaaatc gccttttgct tcataataac ttgtcttcaa
     3361 cggtttgggg agatcgttac caaatgtaaa atcatctggt acattaacta atgttaattc
     3421 ttgtttgggg acaagaattg ttttagcaga tgtttggaca actgggttag gaagcttagc
     3481 accatctgct aatgtaatat tgctggaaag tacagctgcc tttgtcagta tgctgacgga
     3541 ttgtacagaa gtatctacag tatttaattg atagttgtaa gtaagatgaa tggtatctcc
     3601 tattttgctc aatagatctg ctgggacaga aactccttgt tgaatgccgg tatctgcgat
     3661 agttgtacca tttaatttta aggaaccatt gatataagta gtattttcag gaagtgagat
     3721 cgtgattttt ttgtcggtta tcgcatcccc agattttgtt aacgtgaatg tcacattttc
     3781 ttgaatcgta tctcctgata gagcagacag gggatctcct gtctcaggtt gtctgctaag
     3841 gttttttagc tgactatctg cactcacaga tggaaaatta gccactgtta tttgaggaag
     3901 agtggtgatt ccagcttgtt cagactgcca tttgtacgca aaagacggat acatgttagt
     3961 aagtacatcg tccaccaagc gataatcaaa ataatattgc cctgaagcca atgcaggtag
     4021 tgtagaagta gctgtattta cacccccgtt atttgattgg atcgtttgga aaagttgatc
     4081 tgtccatcct gtcgctaatt tttgggttcc tttcgaataa agcctgtaat ggatcgaacc
     4141 cttttttagt tcagttgctg accaattggc cgtgacttgg tacgatgcag aagaaaggag
     4201 ctttccccac tgactatcgg ctgttaagtt tgttggtaaa accgttccgt ctagcttgta
     4261 ctgctccaat acctttaaag caacaggaga aagcaatccg ccttgcgcat ctttcccact
     4321 gtacacgata ttggctgtct gattcaatcc acttgtgctt gctggagtat gagtcagtat
     4381 tgctccttgt gtaacgtttt ttcctgtaaa atagttgact acgcgatcaa agcgactaaa
     4441 tggcaactga tcaaaacgtc cttcagtcag ccccatcgtc aatgtccgac tattggtaga
     4501 gcctggaagg ttctggttat ttccatccac gtattctgtg gtatcagcaa atgtattata
     4561 ttttcctgtt gaatcacttg ttgagatgta actattccac gtatttatct ctgcacttgc
     4621 agaaaatgca tatgctcctg gattcaagtt tccgccagtt aatttaggat aaaatctacc
     4681 tgcagctgga gctaaaatcg aattgaacat acgatcttgg ataatattat tcgaatcata
     4741 aaaatcaaca gaaaaaacgg cctttttaga catagaaggt tgtaatttac ccttggtcaa
     4801 aattttgttt ggactcgtat catatttatc tccgttttct gtcatcttcg cgacattagc
     4861 tgtaacatca aattgaagcg ggatatcgtc cacgttgaat tcgcctcgcg taataatagc
     4921 aataatatct gccaatagag tatttgtatc ccacgacccc atcccaaaac gcaaggtatg
     4981 gcttcctgct gataataaac taccgctagt aaccttgatg ggggcaccgg caatatacaa
     5041 gtaggcgttg ttccagtcaa ttgcttttaa tacatcactg cttgatacat gttccggcaa
     5101 attgatgatc gccacaaatg caaatccttg tgcagtgata ttcaccaaat caagtacatt
     5161 tgttagtttc acatggcttt cccaatgaaa acggaccgtg ccacctttcc aaggatcatt
     5221 tgaaccatcg ttcaaataag tagcagtcaa tttaggcaat tggttagccc cagttagttg
     5281 gacaagcttc ccgccattga ctgcactacg tccaccattt ggaggtacga tattttcata
     5341 cgtgtatgga tcataagaaa tggcaggagg cgttgctcca acagcttctg ccgaaggtga
     5401 aaagataaat atacttaaaa caaaaagcga agtaaaaaag aaggagcgaa aaaatgaaaa
     5461 tttattttgt agcaacatgt tcatcctcct tttcctttct tctccataac aacaaccacg
     5521 caagaagcca taattctata ccaatgatca aataaagaaa acggatcttt tcttcattcg
     5581 tctccgggaa tacgccatgt tgattagatt ctttataaat cggtagctgt gtctgctgtt
     5641 ttgatgcgcc attgactgaa ccacttggat gcggaactgg acttagatca taaaatgtaa
     5701 tcgtacctga acttttggta tctgcttgga ctggctcttg ccagctgaag aacagcaaac
     5761 ttgcaataat ccctcccaat aagatcgatg ttttcaacga aatcacctca tcatgttgtt
     5821 tctccttttt tctttttctt gtggacccac aaaaacagca aaatcaacca taacactaga
     5881 actgcagcaa ccaatcctgc ataaagccat gggaaagatt gttcttgtac tgcaaaaacg
     5941 gaattttcat ttactttttg tgctttttct tttttgatcg taaagttttg tgtaaatcgc
     6001 cattcattac tgtcgcctga ttttgctatg atttctgcgc ggtaatcgcc ggcttttgct
     6061 ttatcaccag ctaaatcaag attcaagtga aaattcgaat ttggtgcaac taaaaattca
     6121 ttagacgtat tttcaatata aagctgattt tcataaaaga tcttgctcgt aaatatcaaa
     6181 ttgctgataa tcctaggctg aggattttgg aaacgaagct gaacagttgg ctttgcgttg
     6241 acctcgtcca aatctcctga taaaagtttc agatccggtt ctatcgtctc ttgactttct
     6301 tgtaaaacga ctgcgagatt ataagcaata cgatttttga tttgtgctcc ttctgtagca
     6361 ttttctgttt cagcttcttc tttaggtgaa acatgaatac ccgctaatac acgtccctta
     6421 aagctttttg ctgggatttt cacttctata ggtatagttg tttctgcatg tgctgataca
     6481 ttgatttcct ttttcgcaat gagcaaaacg tcacggacat caaacggcaa tgtctcatct
     6541 aacgttgggt ttttttgact ataatcgatc gttccaccat cactcgttac cgccggattg
     6601 acagagacct caaatgttcg ttcttcatct gaagaattgc tgataattac ttctagtgtc
     6661 tgattttgtt ccggtgccac taataaatca aaataaccga ttgctttatc tatttggttc
     6721 tctggaaata ccggtttgat tccaaagtct cctgctgaat cctttgcttg gctgattgac
     6781 ggaaaaatga aaaatagact taaaatagaa aaaaacgaaa caaacagtcg tttactttga
     6841 ttcattgtcc accacatcct ttcaacaaca actgcggtaa aaaatccttc acctatagta
     6901 ttttcaccgc agtttgttga atttatttca tactctatgc tactgggcca gccacgaggt
     6961 tccaagtcaa atcagccgtg aatgttccag ctgctgcttt cggcactttc agtgtgatat
     7021 ttgctggatc ataaacagaa agccatgttc ccagaccttg atcttttgct gctgtaaata
     7081 ttggtgtcgc tgcacttgct ttactcaacc cagttacagt ttgactagtt ggtccagtgg
     7141 attcgttatc tgacgtactt ttaacagtgg atgttccaaa atctaattca gcgccttgca
     7201 aaacttgtga accgtttaca aaatctgaaa ccgaaactgt cacgttccac ccttgtgctt
     7261 ggccatctgc acctacacct cgacgatctg atacttggag ataaggtgta tcgttttttg
     7321 aagcgtctac ttgatacgtt tttgttccgc tttcaatctc gtgtgtacca aatggcaatt
     7381 caggaactac atccaatgtt aaagaacctg tctggccagt tgctggattt tccggatctg
     7441 ctggctctac tggttttgaa ggatcagtag ggttaagagg ctcaacggta tttgttggtt
     7501 cagtaaaggt aatatctcct tttgaagtcg cttgaccctc agcaaaagct ggtgttgcca
     7561 agccgcctag aagaagtgtt gaaagtaata atgtcccaat catgttcttt ttcataataa
     7621 atcactcctt tttcttagtg acctaagtat agtagagaca gtatgcaaaa aatcctcatg
     7681 aattcatccg cttacttgaa attatgaccc tttttgtata aaagagaaaa aaacaataaa
     7741 atcgtttaca tgaatattct tcctttttta ttaa
//
LOCUS       DADJYG010000119         7630 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.123, whole genome shotgun sequence.
ACCESSION   DADJYG010000119 DADJYG010000000
VERSION     DADJYG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.123"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            10..2337
                     /locus_tag="JZ382_002095"
     CDS             10..2337
                     /locus_tag="JZ382_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAY2428265.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2463..2792
                     /locus_tag="JZ382_002096"
     CDS             2463..2792
                     /locus_tag="JZ382_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAY2428266.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2832..3836
                     /locus_tag="JZ382_002097"
     CDS             2832..3836
                     /locus_tag="JZ382_002097"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAY2428267.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3833..4279
                     /locus_tag="JZ382_002098"
     CDS             3833..4279
                     /locus_tag="JZ382_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAY2428268.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4429..5244
                     /locus_tag="JZ382_002099"
     CDS             4429..5244
                     /locus_tag="JZ382_002099"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAY2428269.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5450..6622
                     /locus_tag="JZ382_002100"
     CDS             5450..6622
                     /locus_tag="JZ382_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAY2428270.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6784..7278)
                     /locus_tag="JZ382_002101"
     CDS             complement(6784..7278)
                     /locus_tag="JZ382_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428271.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2570 a         1449 c         1522 g         2089 t
ORIGIN      
        1 agaggaggga tgaaaatgat taagaaaact caaaataaaa tccaacgtat atgtcaaaaa
       61 cattttgttt ctagactcaa agctgtatct aaaagaaaat acttacatcc cattatgatt
      121 tcgatcatct tgatttttac tttcttaggg agcttatcaa gtatttatgt aaacgtttat
      181 acagaaacgt caaattttgc taaccaaacg acagaggaga caaatagtcc accctattct
      241 gaaatacagt ccatagaaag caccaatcag tctagcaaca acgagacaac ctcaaccaca
      301 tcctcgactc aactgattga tccgacagaa aaagaaatca cagcagcaaa agcagcaggg
      361 gagaaagctt attcagaaac aggtagaagc cagacattta gtactgttgc ggccactgcg
      421 aatttcaacg gacataccat accagatgga tatacagtga tatatgccaa agatggagtc
      481 attgttgcgg ttaacggatc gatgagtgcg gataaaactg cgttacaagg cgtaaccaaa
      541 gcaatcgctt ttattgataa ccgtccagat atccataatc tgtttattgg ctccacagta
      601 aatggtacta ctatcacatt cccaaaaagt atctatatgg gggtggctac cgctggaaat
      661 acgatcgatt cagttacacc caatgcggat aaaagttatt cttttacacc ctcaacatgg
      721 ggagggcttc ctgccggagg aggtatgagt actttttcaa tcggtataca gttagcaaat
      781 gatattccat tcacggggta ctcgatcaaa aatgggcaga tcgttgatgg taagggaaat
      841 gtttcaaatt ttccaaccta tcaagcagca acggttcagg tcaatgcgaa tcggacagga
      901 actttttctt atacagtcaa tggtggagca gcaaaaatcg gaacgtacgg gactacatta
      961 tctggaatcc atgtgggtga caaagtggtt atcactccaa cagcagtaac gggctattct
     1021 tttgcacaaa caaacggttc tttcacagcc ctagaaagta acgtcgatac ggtgacctat
     1081 acaggcaaac aaacaccaat cactattaag tatcaagact attttggaca gatgattgct
     1141 ccttctaaaa taatgaatct tacttacgga agtgctgcac aagacctgac gactaatacg
     1201 cctactatca acggatatac ctttacagct gtttcagcga tagaaacaaa gaaccaatca
     1261 gcaatgagtg ttttagctag tttagataca aatggaaatg taatcgtaaa ggacgcaaat
     1321 ggaaaacaga tttctgaagt catcctatat tataagacaa atgcaacagt atcaatcaat
     1381 gcaaatggca gtaagtacta tgatggtctt tctgttgtac cgatcgttaa atatgccttt
     1441 aatgataaaa cagaatcaac atccctttta aacaatgatc tcagtattga tcaaatcaca
     1501 tggaatccaa ctgatttcaa agcgatgaat gaaaagggag ttgaagtaac tgcgcctact
     1561 gaaattggaa cctatacgtc ttggcaattg acaaaaagcg gactagctaa actagctgcc
     1621 agaacaaact atttatttac tatcgtgcag acatctgatg tctatacgat taagcaaatc
     1681 agcggtgcgg tagagctcag tggcagtaag acatatgatg gaaaagcagg tgtcccgtca
     1741 atccatgtca agctagcaga gggtgtcact agttcgctta ctccagtaca agtggcactt
     1801 agcagtatgg attacacagc agatgcacaa tcagcaaaaa atatggttaa tgctggcagc
     1861 tatacaatta aactaacgaa tagcggaata gacaaagtca aagcagcaaa tcccaagttg
     1921 tcatttactg atatagccaa cacatcagga acttatacca tctataaagc taacgcagtg
     1981 attactgtcg atgacgctgc ctttaattat gatgcccaaa gtcactcgat ttcggttgga
     2041 aatctccacg ttaccggtgt tgtcttaggc gaatcattgg attataccct aacagttaac
     2101 agccggacag atattggatc acaaatagta acgattagtt taggcgaagg aagtgtaaac
     2161 aataattata cgattatcgc aacagatacc gctaagttga cgatcaatcc gactctcttg
     2221 cttccttcga caggtgggat aggtctactg ccctttgtct ttttaggact gatctttatt
     2281 ttttctggct tcttttattt tatccatcgt aagaaaaaag caggtgaaca gcgatgaaaa
     2341 aaacaaaaaa tgtacgtgtg gatatatttg ctgttctctt ttttatccta ctggtctttt
     2401 ttataggttc tccgactgtg tcagctagag aatcacctat tacatttact ttgcataatc
     2461 gaatgttcga aagcgataag atgccaagta tcagtcaaaa cgacggaata aatatcccgg
     2521 ataactttca aggagtaaaa gggttgaacg gcgttacttt cgctatatat gatgtctcgg
     2581 atgaatttta caagctgcgg tcagaaggaa gtagtgtgga agacgcacaa agaaaactag
     2641 cacaaaaaag tgatagtgaa aaaatactgg cagaaaatgt gacaaaaaca gtagacgagg
     2701 aagagggcat tgcttctttt agtgcatcgg acaaagatga gcaaggtcgt gacgctgttt
     2761 accgatttac ggaaataaag acttcagatt aagtaaaaga aaaatcagca ccatttgtcg
     2821 ttgtgcttcc tgtgacagat tcatctaatc ataaattgac gaccatccac ttgtatccaa
     2881 agagcgaaca aaaaatacct gctgcactca cattaacaaa aacagtagag aataagcaga
     2941 cagattttgc agatggggat aaagtccctt atctcatcac cgtaacaatt cctgagaaca
     3001 tcaacgagat aggaacctat acgatcaaag atacagctga tcctcagtta tggctagaac
     3061 cagaaacgat cgattttttg attggtggag acaagattca tacatttcac acacaaaaaa
     3121 taaaacatgg ttttgtctta tcagattctg gaaaagatct tagttcgttt gctggaaaaa
     3181 aattaacgat ccgttaccaa atgacattga aagaaaacag tgaaaaaacg acatttgata
     3241 acgatgtacg tttgacgact gacaatcaaa cagtagaaac acatcttgcc atccaaacag
     3301 gaggcaaaca atttgtcaaa gtggatcggc aaacgaaaca aaaactagca gatgctcaat
     3361 ttctagtcaa aaaaaatgac caatacttag cgcaagaaaa cgggataaat cattgggtag
     3421 ccaaagatga ttcaagagca acgattttta cttccagtaa agatggaaca ttttctgtcc
     3481 atggattgtc ttttggaagt tacgaactag tggagattaa accgcctaaa ggatatatat
     3541 tgagtcaatc aaccattctc tttgaagttg aaaaaggcag ttatgatccg cttcatccta
     3601 taccattgga aatcatcaac gaaccaaaag caaatccgac gaataatgga aaaacgccag
     3661 ttcagatgca gacaaatggt ccatcaaatg aaaaaaatgg aggaagcttt ccaaaaacaa
     3721 gcgaagagat gcttggaggt ttctcaattt tagggttatt gctagtcctt tcaacaggga
     3781 cagcttggtt ttataaaaaa caacaaaaag gaaacaatag gagggaaata aaatgaaaaa
     3841 catcaaaaca aaaatatgga cgtttttagg aactgctatc atgctgcttc ctttcgttct
     3901 cggcctagga actgcagagg tttctgcagc cgtatcacct acaccagaaa atgttacggt
     3961 taatttgcat aaattgaaat tcacttcagc accagaaaat caaataaaca acggaacgga
     4021 attgacattt ccgaatagcg aaccgttgaa cggcgttgaa ttcaacgtat atgacatcac
     4081 tgctacgtac tatccaagta aagacacagc agttccagca gacgctacac ccttcgcaag
     4141 tgtaacgact tctggtgaag gattagctaa cctgactctt ccaggtaaat ctgatgggaa
     4201 agatgctgta tacgtctttg tagaaacacc aaagcccggt gtcgaaacgt cgccaaatat
     4261 cgtgttaagc ttaccctaga ccctgacaaa cctttagaca cgattcacct atatgctaaa
     4321 aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg ggaacttttc aggggtagat
     4381 aagttagcag gggcgcaatt ccagatcaaa aattcagaga atcaattcgt gaccggtttt
     4441 gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt cagcgttgac attcacaact
     4501 gatactgaag gatccatcag tgtcaatgga ttaatagacg gcaattatac tttagttgaa
     4561 acaaaagcac cagatggtta tcaattaacc aatccacaaa aagaaactac ttttatagta
     4621 aaaaacggaa aattgcaaca aacaaccggt atcatccatg gcgcagatga agccaatctt
     4681 gggaatccaa acggtgtagt caatcgggaa aatacaacta cggataacac gattactgta
     4741 gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac aagatgtgaa cacgcaagaa
     4801 aaattagccg atgcagaatt ttctattcta agagcaaaag atccaactgt taaattaatg
     4861 aaaaaaatag caaaccttgc ctcaggagaa tacccatatg cttggtcaga tgaagtcaca
     4921 ggttctgact gggaagaagt gaaattgatt tctgattcag aagggaaatt ttcaatcgaa
     4981 gggctgaaac aaggggcata ctgccttcaa gaaacaaaag caccagaagg ctacgcatta
     5041 cctaagaatg aagctgcgta cactgaattt acagtagatg atgatccaac aacttctgat
     5101 gaaacaacga tcgataacca acctaaagga atcttgcctt caactggtgg tatggggatc
     5161 atcgtattca tcttggtagg tactgcttta gttggaggag cagtgatcta tttcaaaaaa
     5221 cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa atcatacgat aaataaacga
     5281 tagtgattca aatggggaga aaggttgtga caagtggtcg gtctcttggt attcgctcaa
     5341 caaacggcaa aataggagga actattttgc cgtttgcttc gctaatactc aggtcaaggc
     5401 gctgatttca caacctcttc atatacatat tagaaaatag gtggacatga tgaataaaag
     5461 cagaataaga atactggaca tcttcatggc aatcatttta gtcgttggga tcgggatctt
     5521 ttcttatcct tttgtagaag attcactgaa tgattttctt gcccagcaaa tgatcatcca
     5581 ttatcaaaag caagcatcga ataagaattc agaggaaata aaaaaacagc aggaaaaaat
     5641 gacaaagaaa aaccaacagt tggcagagca aaacgtatca ccagggatag gcagtttcaa
     5701 ccaagcagtt gatgcaaaag cattaaaaga tttacctagt aatgcctttt ttatggaaca
     5761 tatgcttgga gtcatcgaaa tcccaaaaat caatgtcagc ttacctatat tcgatcagac
     5821 aacggaaata tttttgcaaa aaggaacgtc attattggaa ggaagttctt atccgactgg
     5881 cgggaaaaac acccatgccg ttctttctgg gcatcgtgga ctcccagaag caaaattatt
     5941 tacagatttg cctaagttga aaaaaggaga ccagtttttc atccagatca atggaaagac
     6001 attggcgtat caagtagaaa aaatccaagt ggtcttacct gacgaagtag attctttggg
     6061 tatccaaaaa ggcagagatc tcgtgacttt gcttacctgc actccttata tggtcaatac
     6121 acatcgactg cttgtgacgg ggcatcgtat tccttatcaa gcaaaagagg caaaaaaagc
     6181 gatccaagga attgatcaat ggaaaaaatg gaaatttttt atttggttta tcggtatact
     6241 acttggatca atcggcttag tttggctact catcgcttat ttagattctt tggccatcgc
     6301 taagcgaaac tatcctttat ctttttatgt aaagaacaca aacggacgac caatcgaagg
     6361 aatggtcttt tctgtaaaaa cgctcaacgg caaacactat attactagag aaaaagttcc
     6421 atttgtgaaa gcttctgatg aatacggact agtgagattt tctgatttaa aaggcaggaa
     6481 ctatcgcttg caacatgagg agctcctgct taaaatccat gtaaaacata aacattcgaa
     6541 acagttttca atgaaattga aaaaaggaag atataaactg cgaaaagaaa aagaggtgta
     6601 ctatttgatt gagaaagaat gaatgaagat gtccaaaaaa gcagagggaa cagactaaat
     6661 gaaaaaagag agaagctgac gaaatggacg tgtaaactgc tatttttggt ttactatctg
     6721 gaacgccagc ttctattttt ttatgtttca gaagaatttt attattatag tataactttt
     6781 gaattagttt ccataacatt tggagcaagg ggttaaacca cgagcttgag cttcgcttaa
     6841 agtggcaggg gtatacgtac catttccaca tttatgtgta tggtattttg aaccggtcgg
     6901 tgtgacatag acagtttgtt cattattttg ctcaggagct gcggcttgtg cctgctgaga
     6961 agcctgttca gctgctgctt gctgtcgcgc ctgttctgct tgttgctgtg cgattgcttc
     7021 ttgcctcgcc tgttctgctt gttgctgtgc gattgcttct tgttgcgctt gttctgcttg
     7081 ttgtcgtgca atttcttctt gctttttccg ttcttcttct gcttcttttg ctttgattgt
     7141 ggaatcaatt gcagcaagac ggcttgtcaa ctcttgtttg ttttcaggta gcgcttggat
     7201 agctgctgaa gcagttgcat agttttcttt tgtaggattt gcttcagctt gtgacaaagc
     7261 agattccgct tctgtcactt ttttttctag tgcagctttt tccgcttttt gtttctcttc
     7321 ttcttttttc ttttcaattt gttctttcga tgaatctgcc gtactttcgg agatcgtggt
     7381 actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag tagcaccaca
     7441 cccactcaac aaacttcctg cgaccaaaaa caacgcaact aattttttca ctaaatttcc
     7501 tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt acttacttgt
     7561 tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg tgttgataca
     7621 cgggattttt
//
LOCUS       DADJYG010000120         7532 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.124, whole genome shotgun sequence.
ACCESSION   DADJYG010000120 DADJYG010000000
VERSION     DADJYG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.124"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            145..1536
                     /locus_tag="JZ382_002102"
     CDS             145..1536
                     /locus_tag="JZ382_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY2428272.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1609..2256)
                     /locus_tag="JZ382_002103"
     CDS             complement(1609..2256)
                     /locus_tag="JZ382_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2428273.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2264..2950)
                     /locus_tag="JZ382_002104"
     CDS             complement(2264..2950)
                     /locus_tag="JZ382_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428274.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2956..4029)
                     /locus_tag="JZ382_002105"
     CDS             complement(2956..4029)
                     /locus_tag="JZ382_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAY2428275.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4251..4925
                     /locus_tag="JZ382_002106"
     CDS             4251..4925
                     /locus_tag="JZ382_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2428276.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4930..6270
                     /locus_tag="JZ382_002107"
     CDS             4930..6270
                     /locus_tag="JZ382_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2428277.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6419..7405)
                     /locus_tag="JZ382_002108"
     CDS             complement(6419..7405)
                     /locus_tag="JZ382_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAY2428278.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2340 a         1389 c         1324 g         2479 t
ORIGIN      
        1 gcgctaaagg gaatcacacc aattttcata ccaattggtt tcatttatga agttgattcc
       61 ttcgattaat gagcgtttca ttgaaagaaa agggttgagg gagaaaaaca tatgatcagg
      121 ttgttggaat gaaactatag aaagatggaa aaaatcattc gaattctgtc catttctgac
      181 aagtggatga ctaaaaaaga acttgccaaa cgaattggta gttcagaatc tacatttgta
      241 aggtatatcg aagagatcaa aatccgctgg ggaacggatg taacgattca atcttcacaa
      301 aaattggggt atcgtcttga acatttgaat gtaccagctt atttaaatgt tctgatggaa
      361 atgtcgcaaa gttctacaaa cagtcgttta ttaaatgaga ttattgtcaa tccgggtaaa
      421 acgattgagt attattgtga aaaaatttcc atcagccggt cttcttttac acgtaagttg
      481 aaacaatgca atcaaatttt agaaagctat ttattaaaag tgatcgttga ccaaggtttt
      541 cagttaacta gccttaaaag tgagctccag ttgcgtatct ttgttacctt tttcttttta
      601 agttactacg gaagacgcac gttgccattt ccaatcgaaa aaaagaagat caaagaaatt
      661 atggtcaaga ataattgtca gatgaatttg tttcaggaag aaagcccata tgagcaatct
      721 ttctttatta tgtattttgt tgtgcatctc atgagagaat cccaaggaac tccatttgaa
      781 tggaaagctg gagactctta tgtacagttg ataaatatcg ataaaaaaga cttcttaatc
      841 ttgaaaaatc tcttaggaaa attgaagtac agcagctata taaaagtagt gaactatttt
      901 gtcaatacga ttttttcaga attttcggag aaagaaaaaa atgaaatcag tgagcagata
      961 cgaaaaaata tgtcctactg ttcgttttca tccgctgatt gcaaagaaaa aaacgaagaa
     1021 tatgttgttc aattattaac gcaaatttat tttttctccc gtattttccc atacgatacg
     1081 actcatttga cgatccgtgc aacttacttt gcacagcaga tgcaacaccg ccagatacgt
     1141 ctatatgctg gatttcgtgc agaacttcaa ttttattcca ctctactgaa tcagaatctg
     1201 atttattatt tatctagcta tatcttttgg atgcttatcg gtaatcctga aattcaccat
     1261 tatacttctg ataaaaaaat actgattatc agcgacttaa gtcttcgaca tagtcaatat
     1321 attgaagaat atatcagtga tattcttgct gtgcataaaa ttcactctga gacaactgct
     1381 attactgaag atcagctgtc gaaatacaat cttctcgagt atgatctgat cgttacgaat
     1441 caacccattt tgaatgccca tgttcctcat atattgattg atgatagtgt gtcattcgca
     1501 aatgaagaag aactcatccg cttattcgag ttatgaataa tcaaaaaaca gctatgtata
     1561 aaccaccctg ataaaaattt caggacaaat acacatagct gtttcttttt attgtattcc
     1621 ttcagcaatc caacgagatt tatagcgaaa gtcttttaac aactcttctt ttgtccaatt
     1681 atttgcaatg agatccgtca aagtgttcat tacgagggtc atggaaaagt ataggaaact
     1741 aactgcttct tccgacgatt gaatggagaa atgattgatt cttaataatt ccagccattt
     1801 ttcgttcatt ggagaatcag ccgatagttc tctacgtttg gttgtctgct gatcatttcg
     1861 ctgggttaac aagtataacc tttgccaata gatactttcg tgatctaagt cgcagcacca
     1921 ctctaaatat ttttcgatcc ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa
     1981 gatatcttga tggactatgt gagaactgct tttgatcgta tacaaataag catcttccaa
     2041 atcttgaaaa tatttataaa aagcaccgcg cgacatcccc atcttgtcaa cgatttctga
     2101 gactttgact tggctgatgt gctgtgagca aaatatctcc agtaataggt catcgattct
     2161 ctgtttcttt gcgttcgata aattgaaaaa agtttctttt ggcatccaca tctctccttt
     2221 atctctgatt atacgtggat agcccttcat tgtcatgaag gattcattcc ttctttttct
     2281 cttcttctgt caagtaccca tcttccattc gataaacgcg atcactccat tgaatcatac
     2341 ggacatcatg agtaaccata atagtcgctt tttgtttctc atgagcttct ttaactagta
     2401 atttgacgac ttcatatgca tgatctgtat ccaaactagc tgttggttcg tctgctaaga
     2461 tcaagcttgg ctcattatat aaagcgcgag caattgccac tctctgtctt tctccaccag
     2521 aaaggtcttt aggataacta tttttaagtg actggatatc taaagattgc agcaaggaat
     2581 cgatttttgt tgaatcttct ttttttgaag taacttggtc aactagttca aattgttcct
     2641 ttaccgttaa aaaaggaatc aaattagagc tttggagaat aaatcctatt tctttaaagc
     2701 gtaaatctgc tcgttttttt tctggaagct cagttagtga gtgaccgttg atcactattt
     2761 ctccggaagt cggtgtttgc aatcctcctg aaatggtaag gaaagtactt ttacctgaac
     2821 cagaaggacc aatgatgctg acgaattctc cttcctttac ataaaaatca attgctttca
     2881 aagctgagac ctttgtatga ccagaaccaa atgttttggt aacttttttc atcgttaata
     2941 tttttttcat ttttattaac ctcctattgc tgtaattggg tctactttgg ttatcgtacg
     3001 tatcgaaaac aatccgccta gcattgcgac aatgatcaaa atcccactat atagcaacca
     3061 ttgtgaccaa aaaacagcaa aaggcatcgc gcttggtaaa atcagactag tcaaataagc
     3121 aaacaaaaga gcaagcagaa cgcctaccac cccaacaata aaggactgtg ctactaaaga
     3181 tttagcgatg aagaaatttt tgatcccttg tgctttcatt actccaaaaa tagcagtctt
     3241 ctgaagtgtc atcacgtaca tgaaaatacc gaccacggca gcaaccacta agaacaaaaa
     3301 gtatatcatc gcatttaagg tcatattttg ggcagaatag ccaggaatac tttcaatgaa
     3361 tgtcccaata gttaataact gtagaccctt cgatgctttt tctttcgaaa ttttagctga
     3421 atcctttatt acgatcccat tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc
     3481 atacttcaaa gcagtccacg tatctaggga cgcatagatg acgggagaaa ctgtgtaata
     3541 agtttcagga aagataccga caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt
     3601 atctccaatt ttatagcctg aatcagccag attttgagag atgatgattt cattctttgt
     3661 tttaaattca tgccctttcg tgagttttgg taataaaaat gcttctttcg ttgtgccgaa
     3721 aactgtaaca ttttctttgt tttttccttt gattgctccg ctgtacaatc cgattggtgc
     3781 tttctctcct ccttcaatgt atttcagatc tcctcttgtt aattgtgaag ctgcaaatac
     3841 ttgatttgca tcctccgaca aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt
     3901 aaactcttct gaaagtccat tcgctagtcc tgacaacatg aatacgacat acgcaatcaa
     3961 gaacataata ccgacgatca agccatatcg taatttagaa tagcgaattt ctttgatagc
     4021 tagaaacatt tgtttttcct ccctatgata gattgatagt gacaaaatgt cacttgttga
     4081 atagtgtaac tctaaagtga caatttgtca cttgtgttgt ttttttctta tcaataaatt
     4141 gtctaagaca tctttcgata aaaccttaac atgagtcata aaaaatttat ttaagcctta
     4201 tttatgcttt ccatgataaa ctaaagtgac caaatcaatg aggtgggaaa atgattaaac
     4261 tattagtagt agaagacgat aaagtattat ccaataatat aaaagaaata ctcgctgcaa
     4321 tcggcgagat aacacaagtt tatgatggta gtgaaggact gttcgagttg gaaagtggag
     4381 tttatgatct tgcaatcttg gatttgatga tgccggaaat gaatggatat gaagtgttag
     4441 caaaaatacg gaaagaaaaa atacagactc cagtattgat tttgactgca aaagatagtt
     4501 tggaagataa gattgaagga tttgaacgag gtgcagatga ttatctaaca aaacctttct
     4561 atcgagaaga actgatcatg cgcgtgaaag cattattgaa acgctcgcta ggtctctttg
     4621 atgaaaatgt tctgaagtat aaggatatca gttgtgattt aaaaagcaat gaagtcttgt
     4681 ataaagaaca agttttgccc atacaaggaa aagagtttga attgctcgtt tattttttac
     4741 aaaataaggg aatcattttg acaaaagagc agatatttga ccggatctgg ggatttgatt
     4801 ctgatacgac gattacagtt attgaagttt atatgagcaa cttacgaaaa catctgcgaa
     4861 gtactggatt ggataaagaa atcaaaacct tacgtaatgt cggttatata ctgcaaaggg
     4921 tgtgatgcaa tgaatggaga tattacacga aagcaacgta gtcgcttttt tgcgacaaat
     4981 gtagcagcat ttgcttttat cttttttctt ttgggattca ttactttaca gatattaaac
     5041 caatctgcat acagagaaac agatctgtca ttagagaaaa taaattttga ttctcgaaca
     5101 gtacaattag agatacaaag atatcagcaa ggcgatccct ttttaaaccc gactcaaacg
     5161 gaacaaaaca gaacaaacat ggaacagcca ttaatggaac catcaaatga ccgttttaac
     5221 actcaagtga tcttgtggtc taaaaatggt gagatattga ataaagcagc tattggtggg
     5281 cgtttcaacc aaatcaaaga attaaaaatg gatacgaaaa atctgaatac catccaaaat
     5341 attgaattaa atgaagacag tggctcatct gccctctctt tccgttcgat tacgaaaaaa
     5401 gcggagacga atcaagatgg cgttgcctac atccaaattt tagaaaatac aaatcaaatc
     5461 aagaattcat tagcgacttt tcgcacgatt atgattttgt gtatgattgt tttttggatt
     5521 ctctctattg gtatcagtta ttacttgtct agtctaagca tgcgccctat tattctttct
     5581 tggagaagac aaaaagaatt tgttgagaat gcttctcatg aattgcgcac accgttaacg
     5641 attattcaaa atagtttgga acatcttttt accaagccta atcatactat tattgaagaa
     5701 tccgaaagca tcgctcaagc attgtcagag acgaggcgcc tcactggatt gacttcggat
     5761 ttgctgacaa tcgctcggaa tgattctgat cagcagctgc tttcaaagca aatgatcaat
     5821 acacaagaat atatcgaaca acttgttaaa ccatttcaag agatggccat catagatgga
     5881 aaagagttta ttctccataa cgatgcaaca ttagaagtac caattgatga aaaaaaaatc
     5941 catcaagttt tggttatttt gctggataat gcattaaaat atacgaatcc agaagataag
     6001 atcattttgg attcatctgt taaccataaa gaatggctga ttcacgtaaa gaatactggc
     6061 ccttctatca gtgaagaaga tcaagcaagg atttttgatc gattttatcg ggaagatcaa
     6121 tctcgggcga aagagactgg cggatacggg cttggcttgg caattgcaaa acaggtaatc
     6181 gaacagcatc atggacagat ttctgtatcc aattatcttc cacaaggagt cgattttaaa
     6241 gtccaattgc cgttgaagcc taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa
     6301 aatcttgtca cagccctttt ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg
     6361 aaataagccg aaatttcttt aagctgacca cttctgtcac aacctcttta gcttacgctt
     6421 acgatttttt attgcggtct tcaatgtgta catcaccgct tgtgtcaata tccaattctt
     6481 ctcggctaac atcttctgtc actttttcaa catcttcatg ttcttcttta tgaatcccaa
     6541 cttcttcacg aataactgta tgtttgctta catctacttg ctcttctttg attgggatag
     6601 taatcgtgtc atcttccaat ttttcgtctg ttccctgtga agattgatct gttactggtt
     6661 tgcgttcgat gacgatttct tcacgtttga ccggtacttc taccgtttca gtatcgttga
     6721 ccacatgttt gtggatgtcg acttctccag ttgtcacatc atgtgtattt acatctaatt
     6781 tttcttcttt tagttgaatt gtatcatcat ctagatttgg tttgcgatcg atgtcttcag
     6841 tcttgcttgt acccattgta tcagtctcgc caggtacgtc catcggatca gttcctggtg
     6901 tcatattcaa tggatccgtt gtattttgaa agcctgtcgt gccacctacg tccattggat
     6961 ctgttccagt gccagtttct aatccagtcc ccattgtgct tgctgggcta acagtatctt
     7021 gtgtatccat tgtatcttct gttgtttctg gctgacgata gccttttact gtgatgacaa
     7081 attttccatc agaaatatct cctctatatt gatataaagg atcattttct ggtgtagccg
     7141 tctcagaatc ataggtatca aaagaaaaag tatcttttat tttttcccat aaagaacggt
     7201 cttcttcacc atccatccga ttttcatctg tttcaacatc tataccagac aatccgtcaa
     7261 atcctagtct agatgctgct tcatcagtag tataaataac gatatcatct cgttcatacc
     7321 cttcgttacg caaccgttca attactgata atgcttcttc cctcgtacta tagcttccga
     7381 tgaccgttaa atttttctct tccatgtaaa ttcctcctag caaatagatt tatgattggt
     7441 atattgttac ttataaattt attctatagg agacgattgt tttctacaaa tatttagatt
     7501 ggaatttgat taaaacaatt taattgaaat tt
//
LOCUS       DADJYG010000121         7443 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.125, whole genome shotgun sequence.
ACCESSION   DADJYG010000121 DADJYG010000000
VERSION     DADJYG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.125"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(216..1664)
                     /gene="cls"
                     /locus_tag="JZ382_002109"
     CDS             complement(216..1664)
                     /gene="cls"
                     /locus_tag="JZ382_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY2428279.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1816..2709)
                     /locus_tag="JZ382_002110"
     CDS             complement(1816..2709)
                     /locus_tag="JZ382_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2428280.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2693..3379)
                     /gene="ftsE"
                     /locus_tag="JZ382_002111"
     CDS             complement(2693..3379)
                     /gene="ftsE"
                     /locus_tag="JZ382_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY2428281.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3484..4585)
                     /gene="prfB"
                     /locus_tag="JZ382_002112"
     CDS             complement(join(3484..4521,4523..4585))
                     /gene="prfB"
                     /locus_tag="JZ382_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY2428282.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4711..7245)
                     /gene="secA"
                     /locus_tag="JZ382_002113"
     CDS             complement(4711..7245)
                     /gene="secA"
                     /locus_tag="JZ382_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY2428283.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1997 a         1649 c         1296 g         2501 t
ORIGIN      
        1 aaaagtttct atgctatact ggctttgaaa tgtgtctctt ttaatagcat atattagctt
       61 atttcttgct ttccccaaaa aacaagagat accccaaagt taatttcgct catgcgagac
      121 gctttctcca tttctgacgt actctcagaa atggccccgt tagaaaagga cacgagatct
      181 ccatctgttg atggttctca tgtccttttt atgttttaaa ggagcggcga aatcaatctt
      241 gctaatcctt ctttcatctt gatgattatt cctcggtttt catacatttc ttttgtgaga
      301 tgcgtagaaa ttttcgagtc ttcaaaaaaa gcttttctta cacgtgaagc cattcgttca
      361 tcataaacca gtgtattgac ctcaaagtcc aagcgaaatg aacgaacatc gatatttgct
      421 gagccaactg aaacaatgcc accatcgata atcatcgtct ttgcatgaat aaatccattc
      481 tcatatgttt cgatccttgc tccatactcg attagctctg cagcgaaaga atacgttgcc
      541 cagtaaacga gaagatgatc tggtttgtta ggtatttgga tatgaacttt tacgcctgaa
      601 agaagagcca acttcagtgc ttcatgaatc gatccatccg gtatgtaata aggcgtttgg
      661 atcaatatct cccgtttggc taacgaaatc attttcaaat aagtcatttt gatttgttca
      721 tgttcggtat ctggtccgct tgtcacgatt tgaacagcta tatcaccagt agagtgaatg
      781 gacggaaaaa atttgggaga atagcctact tcttttgtat gctgagaatt ccagtccatc
      841 aaaaaacggt tttgcaaggt atacaccgct tctccgtaaa tacgcaaatg attgtctcgc
      901 caataaccaa atttctttac caatcctaga tattcatttc caacattgaa cccacctgta
      961 tatccgattt tcccatcaat aactacgatt ttacgatgat tgcgataatt gatccgagga
     1021 ttaatatacg gaacaaatag aggaaagaaa aatgcgactt ctccgcctgc ttttttcaaa
     1081 ttctgaaaaa agctggaaga aacttgtgtg gaaccccatg catcaagaag gactctgact
     1141 tttacccctc gtcttgccgc ctctgtcaat gcatctcgaa cttcttcacc taatgcatca
     1201 ctgcgataaa tataatactg gaagtggata tggtcttctg cctgcttgat atcttcaatc
     1261 agcgcatcga atttttcacg accatctgta aataaagtga tttcattttt agtcgtatag
     1321 agcgtacttt caaataccgt gagcatatag atcaattgtt tcacatctac ttgtccagta
     1381 ggcggatgag gataaagccc tcgcatcaat gcctgtttgt cttcttcaat ttccaggttc
     1441 ttaccgattt tcccttggat cttcaaatca aagattttct ctttggaaat ccctcttcct
     1501 aaaaaaatat ataaaataaa tcctacaact ggaatgaaca tcaaaacgag cacccatgcc
     1561 catgtttgag cagtttcctt tctttctcga aaaacgatga taaaggaaag caaaatattc
     1621 ataatcaaaa ggatcgtaaa aaaattgtca acgataattt tcacggcttc acctcttctt
     1681 ctaccaactt aagaataccc aagttgtttt ttaaagtaaa gggaaaaaag ccgcttcctt
     1741 ctattctcct agccaataaa aaaagaaagc tggaacgaaa ctgtccaact ttctcatttt
     1801 atttatattg tggtattaga atttcaagaa acgtctcata gaaatgatcg agccaagtga
     1861 accaatgatc acaccaatgc cgaccattaa aagattgatt ttccaaacga tattttgtgg
     1921 atgaatcaat gaataatggg aacgtaataa tgatggattg atcaattgga aaaaccatgc
     1981 ataaccaaat gtgatgatca aaatcggaag aatcgcaccg atcagaccga tccagccacc
     2041 ttcaaggaaa aacggccagc gaatatatcc attttttgca ccgactaaac gcatgatttg
     2101 aatttcacgc tgtctagaca aaatcgtgat ccgaatcgtg ttcgaaatga gaaacatcgc
     2161 tacgaacagc agcaatgccg ctgcaactaa tccccatgtc cgaactgcac cagcgatttg
     2221 gaaaatccga tctgaggaag ctccaccgta atcagaacga gaaacattct tcaaatctgc
     2281 tgcttcttct gctacttgtt ttgtataaga aggatcagta gcacttacta catacacatc
     2341 atataacggg ttattgtcac cttcaaacaa tttccaggaa tcacccattt cgttttggat
     2401 ttttttcaat tcctgttctt tgcttgaaaa ttcaactttt ttaacatgat ctaagtcatt
     2461 caattctttt tctagctttt tcatctcttc gtcttttgta ccgatatcta cgaatacaga
     2521 gacatcaacg tttccttcta tatcttgagc taatttagtt gcgttcatga taactgccat
     2581 gaatacaccg actaaagtca atgtgatcgt taccgcactg actgaagcaa ctgtcatcca
     2641 gccgttacgt ttcaaactct tgatactttc taataaatgg cggaaaaagg ttctaatcat
     2701 cgtatccata ttccccttcc gcttgatctc gaataatgcg gccgttttcg atagcaatta
     2761 ctcggtgccg aatcgtattt acgatcgtac tgttatgtgt agccatcacg acagtagttc
     2821 cttgggcatt gatccgatct aataatttca tgatttccca tgagttttct ggatccagat
     2881 ttcctgtcgg ttcatccgcg ataagtactt taggcgtatt cactattgcc cgagcaatag
     2941 agacacgttg ctgttcccca ccggataatt cgctaggaaa gactcgaact ttgtgtttca
     3001 aaccaacaag gtccagcact tccattactc gttttttgat atcccgaggt tttctgccga
     3061 taacttgcat agcgtacgca acattttcat atactgtttt tcttggtaat aatttatagt
     3121 cttggaacac cacgccgatt tctctgcgta gataaggaac ttcgcggttt ttgattgcta
     3181 ttagatcatg acctgcgacg ttcaaagttc cttttgaagc tttttcttct cgatacatca
     3241 atttgatgaa tgtagattta ccggctccag acggaccaac cacatagaca aactctcctt
     3301 gttcgatgac aattgagaga ttgcggatag cggtcgtacc atttgagtac ttcttcatta
     3361 catccttcat ttcaatcatg gcgttctctc catatctttt tcagtcttta gctattatag
     3421 catggcaatg tttcaacatg cttgcaaaat cttacaattt tatttcaatt taacaatggg
     3481 ttcttattgt agatgcattt ttaaatagcc gtcaataaat aaatcaaggt caccgtccat
     3541 aactgcttgg acatttcctg tctctacgtt tgtccgatgg tcttttacca tagaatatgg
     3601 atggaaaaca taagaacgga tctgtgaacc ccagccgatt tctttttgtt ctcctcgaag
     3661 tgcagctgct tcttgttctt tcttttccac ttctaattga taaagcttag cttttaacat
     3721 ccccatcgct tgctcacggt ttttcaattg tgaacgttgt gcctgactag ctactactgt
     3781 tcctgtaggc aagtgagtga tccgtacagc agattctgtt ttattgatgt gctgaccacc
     3841 tgcaccactt gctcggtacg tatcgatttt caaatcatct ggattgattt ctatatcgat
     3901 cgtttcatca agctctggca tcacatctac cgaacaaaaa gaagtatgac gtcgtttcgc
     3961 tgagtcaaaa ggcgagatac gtacaaggcg atgaacacct ttttctgatt tcagataacc
     4021 aaatgcgttg tgtcctttga tcagtagggt cacactcttg attcctgctt catcacctgc
     4081 ttgataatct agtgtctcta cttgataccc atgctgctct gcccagcgtg tatacatacg
     4141 taaaagcata ctgccccaat cttgggactc cgttccgcca gcccctggat gcaattcaac
     4201 gatggcgttg tttcgatcat aaggaccatc taacaacata gataattcat atgtcttcat
     4261 tttttcatct agcgcattga tacgtgtttc tgcttcttct tgcatggctg catcaggttc
     4321 ttcctgcaac atctccacca tcaattcgat ttcttccagt tcttcagcaa gagaatggaa
     4381 ttgatcataa gtttctttat ttgcattgtt ttcactgatc actttttgag cagcttcgct
     4441 atcatcccaa aaaccaggtt cagccatttt tgcttctgct tccgcaatgt cttcttccag
     4501 ctggtctaag tcaaagagac ctcctgaagc ttgtgacctt ttcctgcatt tctgtcagga
     4561 gatttcgaat ttcggatatt tccatgtttt ttccttcttt cactatactt ttttcattat
     4621 atctagtcta acagaatcag caaataacag aaagaaggtg agacgaattc ttaaaaaaag
     4681 aatttgtctc accaatcctg ctgtcagaaa ttagtttgca ttttttccat gacagttctt
     4741 atattttttc ccgcttccac aaggacaagg atcatttctt ccgaccttgt tcacatggat
     4801 tggctgtttt ttcgcgctag tattactatt ttctttggca tctccgtctt ctgagtgaga
     4861 agctgttcct tgagaaacct gttcacgctg tacgttttga cggatctcag ctttcataaa
     4921 gattcgagtg acttcatact cgatggcgcc taccatatct tcaaacatct tatagccttc
     4981 tgtttgatat tcgactaatg gattgttctg tccataagca cgtaagccga ttgactgacg
     5041 taattgatcc attgcatcaa tatggtctgt ccatttagag tccacaacac gcaagatcac
     5101 gactttttca aattccaaca gttgctctgg accatttagt tgagccgctt tggtattaaa
     5161 tacttcttgt gctcgttcgt ttagatactc tttgatctcc tgagctgttt ttccttcgat
     5221 atctgcaaga gtaatactat cttcatggac aagagtactg ccggcaaaat cgatgatact
     5281 ttccaaattc caattttctt tctctaattg tgtatgcgca tctactacac gactgattgt
     5341 ccgtttgacc atgttcatta gtggcttgga taagcttttt tcttccatga ttacttcttg
     5401 tcgctgttta tagattactt cacgttgttc acgcatcaca tcatcgtatt gtaagacgtt
     5461 tttacgtgtg tcatagttat ttccttctac acgtttttgt gcagattcta cttgtttcgt
     5521 cagcatcttg ctttggataa cagcatcttc ctcaccgatc ttcatccgat ctaagaaaga
     5581 tttgattcgt tctgaaccga atcgtttcat caaatcatct tccaatgaca agtagaactg
     5641 tgacatacca gggtctcctt gacgtcctgc acgtcctcga agctggttat cgatacgacg
     5701 agattcatgt cgttctgtcc cgataactgc tagaccacca agttctctta ccccaagtcc
     5761 taacttgata tctgttcccc gaccagccat gttggtagca atcgtcacag cacctttttg
     5821 accagcattc atgatgatct ctgcttcttt aaagtgattt ttcgcattca gcacttcatg
     5881 aggaatacgt tcttgattca gcatatctga taataactca gatgtttcaa ccgcaacagt
     5941 tccgactaac acaggttgac ctttgcggta gcgatctttg atatcttgca cgacagcgtg
     6001 gaacttgctt tgtaatgttg ggtaaagcaa atctggacga tcatcacgga taaccggacg
     6061 atttgtcgga atctggtata cttggatgtt ataaatttct ctgaattctt cttcttctgt
     6121 tttagctgta ccagtcattc ccgcaagttt cttgtacata cggaaatagt tctggaaagt
     6181 gatcgtcgcc atggttttcg tttcgtcttc gatctctacg ccttctttag cttcgattgc
     6241 ttggtgaagt ccatctgaat aacggcgtcc atccatgatc cgaccagtga actggtcaac
     6301 aatcagtact tttccgtctt ggacaacata atcgatatct aaaagcatga tatagttggc
     6361 tctcaatgct tgatccaagt gatgggtcaa tgcagtattt tcaatgtcgt ataagttatc
     6421 caaaccgaat gtttcttctg ctttttcgat ccctgcttcg gttaaactga ttgtttttga
     6481 ttgtacatcg attttataat cttcttcttc tttcaaacgt ttcacgaagt tgtctgctct
     6541 tgtatataaa gctgtggatt tttctgcttg ccctgaaatg atcagcggcg ttctcgcttc
     6601 atcaatcaag attgagtcaa cttcatcgac aatggcataa ttcagcggac gttgtaccat
     6661 ttgatggcgg taaacgacca tattgtctct caagtagtca aatcctaatt cattattcgt
     6721 actataagtg atatcacaat tataagcctc gcgtttttca tccgaagatt ttgagttgat
     6781 attcaacccc actgttaatc ctaagaaatt ataaagttcg cccatttccg tcgagtcacg
     6841 ggttgctaaa tattcattga cggtaactac gtgtacccct tcgccagtca gagcatttag
     6901 atatactggc atcgttgcgg tcaaggtttt accttcacca gtacgcattt cagggatatt
     6961 tccatcgtga agcacgattc cacccatcaa ttgtacgtgg tgtggatata accccaatac
     7021 acgttttgct gcttctcgga cgactgcaaa agcttccgga agtaattgat ctaatgtttc
     7081 tcctttttgg tatctgccct tgaattcagc agtttttgcc tgtaattgtt catcagttaa
     7141 tgctgccatt tggtcagcgt atgcttcaac tttttttgca atgctatcta aacgttttag
     7201 ttctttctta tcgttttcga taagtgaacg aatcaaattg gccatgcgtt gtgtaatctc
     7261 cttttccttc tgtgtattat ccagactatg tagactttac ttttcatttt atcattagtt
     7321 atagataatt gaaatatctt aagcgtatta aagtaagaac ttcataattt attcatattt
     7381 catctaaaag aggaggaaac tcaaaaaaac ttgcggcagc tgtcagtagc tgctgcaagt
     7441 ttt
//
LOCUS       DADJYG010000122         7083 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.126, whole genome shotgun sequence.
ACCESSION   DADJYG010000122 DADJYG010000000
VERSION     DADJYG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.126"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..561)
                     /locus_tag="JZ382_002114"
     CDS             complement(<1..561)
                     /locus_tag="JZ382_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAY2428284.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="JZ382_002115"
     CDS             complement(748..1398)
                     /locus_tag="JZ382_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428285.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="JZ382_002116"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="JZ382_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAY2428286.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="JZ382_002117"
     CDS             complement(2910..3908)
                     /locus_tag="JZ382_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAY2428287.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="JZ382_002118"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="JZ382_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY2428288.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="JZ382_002119"
     CDS             complement(4924..5655)
                     /locus_tag="JZ382_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAY2428289.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="JZ382_002120"
     CDS             complement(5659..6924)
                     /locus_tag="JZ382_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAY2428290.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         2132 a         1505 c         1097 g         2349 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtg
//
LOCUS       DADJYG010000123         6991 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.127, whole genome shotgun sequence.
ACCESSION   DADJYG010000123 DADJYG010000000
VERSION     DADJYG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.127"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            5..190
                     /locus_tag="JZ382_002121"
     CDS             5..190
                     /locus_tag="JZ382_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428291.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            695..889
                     /locus_tag="JZ382_002122"
     CDS             695..889
                     /locus_tag="JZ382_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428292.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSGF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1080..1329
                     /locus_tag="JZ382_002123"
                     /pseudo
     CDS             1080..1329
                     /locus_tag="JZ382_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1573..2403)
                     /locus_tag="JZ382_002124"
     CDS             complement(1573..2403)
                     /locus_tag="JZ382_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAY2428293.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2611..3945
                     /locus_tag="JZ382_002125"
     CDS             2611..3945
                     /locus_tag="JZ382_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY2428294.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTYTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3986..4897
                     /locus_tag="JZ382_002126"
     CDS             3986..4897
                     /locus_tag="JZ382_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY2428295.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4894..5748
                     /locus_tag="JZ382_002127"
     CDS             4894..5748
                     /locus_tag="JZ382_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY2428296.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5745..6362
                     /locus_tag="JZ382_002128"
     CDS             5745..6362
                     /locus_tag="JZ382_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAY2428297.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6364..6882
                     /locus_tag="JZ382_002129"
     CDS             6364..6882
                     /locus_tag="JZ382_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY2428298.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2099 a         1203 c         1395 g         2294 t
ORIGIN      
        1 atgaatgagt aaaaattcta aaggattcct aaccatctta ttagctttta tagggtatat
       61 gttggttggg ctgctaaaat catacagtaa tgagttgctg aatttttcaa catttattaa
      121 tgatacatta gttccaagtt tgtttttcat tgtatttttt gctgttggtt attttataat
      181 aaagatatga tgactaattt agctcaacta ttccagcgtc agctgaagat attgcaattc
      241 ccgattcctt aatcatttat ctggatgaga cagaaagtga agttgcaaca gatgtgcatg
      301 tagttgagga taaaaatgat agtttaaaaa ttaatagttc agatacttta ctaagagtaa
      361 gtcctatctt ttcagctgtt atttagatgc aagtagcaac taagttaggt agtactcctg
      421 caatggatag gtatttgtgt gttcgatata taagtggtcg aagtatcaag gttatgtata
      481 ctggatggga gatagaagag ttaaaaattg gaggtcaccg ccacaaatgg aatttagatt
      541 tagaggtacc ttagatttgg tatagttatt aaacacaaga tggcctggtt tgtagggaaa
      601 gtcgaagagt atttttccac ttctgcgccc ctttaatttt tctagtaatg ccgtgaattt
      661 gtgattaatt ggaaatatga agaaaggaga gtctatgaaa acaacactag ttctattcta
      721 taaaaagcat ccttatttta cacttctgat aaatatattg ttggctagtg ttatcgggat
      781 ttctgttgaa tatctaataa ataaagattt cataggttct ggtttttata cggctttatt
      841 tcttggactt cttgaagcat tttccattta taaaaaatca aaaaaataaa atagattgcc
      901 gtaagagatc tttttcattt catttttttc tgaaaacccg ttgtttctgt cagtgaagca
      961 gcgggttttt agatttattg tctttagacc taattgagcg cgccgagcga ttgaaataca
     1021 aaaccacttg ctatgcgggt ggagcaataa agggaaatag tttagcccgt acaaagtaga
     1081 tgtgtaaata ccacattgta ttcacaccaa agtatagaag aaaaataatt tataatcaat
     1141 atcgagccaa tattcaggag atcatcaaga gattgtgcaa atctaaaata taaatttggg
     1201 tatcgacact tttgggtgga aggatactat gtgacaaaag taggacaaaa tgaagcgacc
     1261 atcaaaaagt acattcaaga acaagaaaaa catgatagtg cgttagataa gctgagtgta
     1321 cgagagtaag acgatccttt taagggctaa ggagtagtac ataaaccttt taagaggtgg
     1381 gcaaaactgg caaaggcaat atggcttgaa cataaaatat gaaagccagc gtctttaggc
     1441 gctggccggt gatttgagct tatagctcta gagaaaacca cccttttgaa gggtggttat
     1501 gattaatgac tttagtcagt tttgctcaca aagcgtgtgg aacaaaaaac gggtgtttct
     1561 acgggtagat atttatttct tgaatggagg tgtcccatac atttttggtg ttactggttc
     1621 taattttggt gcagcgcctt cgatcttagg ttctttcaac cattgatatt cgccttcgcc
     1681 atccagtgct ttgccactag cccaagaacc ttcactactt tggtctccct ctgagaagtt
     1741 atagaagtca taagcaatgt cttttcgttc tttgtcacgc gggaaagtac ttgggactaa
     1801 gattgaacct tctttttctt ctaattcttt cactgcacat tcccatagat tttgatgata
     1861 gctatctctt gcaaggagaa cagacagcat atcttttacg ccacgatcat cgatcatttc
     1921 gtaaatacgt gctacttgaa gtcttccttg tgattcggca tttaaattag ctctaaagtc
     1981 tgctaacaag ttgccactcg caatgatata ggcaccgctc catggattgc ccacgctgtc
     2041 agcaggacga ggtcctaagc cagctacgat cgcgtgctgt ggattcattc cagagataat
     2101 ggcaccgatt gttggatctt tttgcatcgc tgcttcttga tcttcaacag gcgcaccctc
     2161 taataaacga gcaaccattg tcgccaacat ttccacgtgg ccgatttctt ctgttgcaat
     2221 gtccatcagc atatctttat atttttcttc tcctcggcag ctccagcctt ggaacaggta
     2281 ctgcatagct acactcatct cgccatattg gccgccgatc agctcctgca gatatttcgc
     2341 gattaatgga ttcggacgat ctggttttgc ttcaaactgc aattctttct ggtgtctaaa
     2401 catcaaaatt cctcctaatc tatttttaat acattctcat tctgctacga aagattaaaa
     2461 aattcaaaga aaacgcttgc gaattatata aagtattggt atcattaata atttttttag
     2521 aatcatacct tataaattgt tattttcaga acaaaatgat atcgttatat taaaaccgac
     2581 atcggttaca ttaaaatttg gaggtaaaat atgaaaaaat tattgtttac agggttgctg
     2641 gcaagttcag cattgttttt attaggagga tgcggatcaa gtaatgcaag cgatgccagt
     2701 tcaagtgcag caaattcttc tgaaaaaaca agtgatggaa gaaaaaatat tgatttctgg
     2761 tccttttggg gatccggtgc acgaaaagaa gctatcgaag aaatcattga ggatttcaat
     2821 caatcacaaa acgagatcca tgtaaattat aagtatcagc catggggtga catctggact
     2881 aaatctttat ctgcaataac agctggaaat gcacccgatg ttattgtaca ggacataaat
     2941 tccgttgccc aaagagcaga agcacagcaa gcaactaatc tctccaaata tgtagaaaaa
     3001 gacacttcta aggattttta tccgcagctt tgggatacag tagaatatga aggtgatcct
     3061 tatgcgatcc cattcaatac atatacacaa gtcatttttt ataataaaaa gttatttaaa
     3121 gaagctggaa tcagtgaaca agatctaccg acaacttggg atgaattaga agaaacagca
     3181 cgtaaactag ataaaaaaga aggaaaagaa tttacacgca tcggttttta tccattatgg
     3241 aatttagggg ctgatgtatg ggcgctaaac gcagataacg ggactagttg gtttgacaaa
     3301 gatgaaaaag tcaagattga tactaaaaat aaagtggaag ccttggagtg gatccaagac
     3361 tggcaagatt attatgggaa aaatacgatc aataaattag aagccgaatt tgggtctggt
     3421 gtatctgatc catttatttc cggattagta gccatgcgtg cccaaaatat caattattac
     3481 tctagtttga tggaaaatgc accggatgat tttgattttg gcgtgatgca gctcccagaa
     3541 aaagaagaag gttcaggcca ttggtcgtgg ggtggcggat tcgtcttgga agtaccatac
     3601 ggagcaaaag atcctgaggc ctcttatgaa ttcatcaaat atctttcttc tccagaagta
     3661 caagaaaaat tcggcgaaaa aagttttgat atcatggcga gcaagacagc caatgagaat
     3721 ttagtgaata atgacaacct aacggataaa ggaaaaatga tctatcaaat ggcggacgaa
     3781 aactttaaaa atacggtgat cacaccagtt cctttagatg ctccggatta cacgacatta
     3841 gtgaatgaac agattgacca gattctttta ggaacaaaat caccaaaaga aggattggca
     3901 gatgcacaaa aagcagtaga aaacttagtc aagcaaaatc aataatccat ttttgactgt
     3961 ctaggtacag aggaggcaag aaaatttgaa gcgtttatct atccgaaaga aaaaagaagc
     4021 aagatgggcc tatttattta tctcgccttt tattatcggg ttcttggtat ttatgctagg
     4081 gcctatgctt ttttcagtta ttggtagttt caccgattat aatttgacat ccaagatgaa
     4141 tttcattggt ctagcaaatt tcaaaagaat gttcgttcac gatgatttat tttggaaatc
     4201 gttatacaac accgtttatt tcgtcatctt caatgtaccg ctgacgacga ttttcagtgt
     4261 gtttttagcc acagtactca atcaaaaaat caaaggaatc agtgtattcc gtacagcttt
     4321 ctatttaccg gcagtcttat caggagtagc agtctacatc ttatggatgc agctgctttc
     4381 tccatctgca ggattgatca acacatttct gggctggttc catatccctg gaccagcttg
     4441 gctgtttgat ccacaatgga cgaaacctgc gttgattttg atgaaaatat ggagttctgg
     4501 aggagcaatg ctactttact tagctacatt acaaaatgtt cctagatctt tatatgaatc
     4561 agcagaaatc gatggagcag gcgtttgggg aaagttcaaa agtattactt tgccattaat
     4621 cactcctatt attttttacg atgtgatcac ctctacgatc ggttctttcc aaatttttca
     4681 agaagcttat gtcatgacga aaaatggaac aggcggacct gcaaattctt tgctttttta
     4741 taatctgcat atgtggaaca aggcgttcgt agcttttgat atgggttatg cgatggctat
     4801 gtcaatgatc ctgtttctga tcgtgttggt tctgacgttt gtcaatttga agcttgcacc
     4861 taagtgggtc tattattctg gaggtgacgg caaatgatgg attatttcaa aaagaccagt
     4921 gtgaatcggc aaacaaaaaa aatactaagt tatgtcctca tgacagtaat aggaatcatt
     4981 ttgatcattc cattgctttg gatggtgttt acttcattga aaccaatgga agaaatcgtt
     5041 cgttatcctc cgaccttttt cccagaaaaa atcgtatggg aaaactatct ggatacgatt
     5101 gccgcctttc ctttttggcg ctatgcaaga aatacattat tcatcactgt gctagtagtg
     5161 atcggtaatg tcctttcaaa ttcttttatt gcttatggat ttgcgaagct tgattttcct
     5221 gggaaaaagc tgatgtttgc tttggttctt tctacgatga tgatcccggg gtttgtaacg
     5281 atgatccctc aatatgtatt attctccaag atcggctggg taggtactta tttgcccctg
     5341 atcgttccat cgttcttcgg caatgcgttc aatatatttc tgatgcgtca attttactta
     5401 tcgatcaata atgaattgat cgaagctgct gaaatcgacg gagcgaatca tttgtatatt
     5461 tggagtcatt tgatgcttcc attgacgaaa cctgccttga ttaccattgc gatcaattcc
     5521 ttcaacgcag cttggaatga tttcttaggt cctctgttat atatccaaga tcaagaaaaa
     5581 tacacgttac agattggctt gcaagttttt cagaaccaag caacgacaca atggaattat
     5641 ctgatggcag gagcaacact tgtcttgatc ccgacgattt tgctgttctt ttttgcacaa
     5701 cgttatttca ttgaagggat ggatttgaca ggaggttcaa aaggatgatg aatcgatcga
     5761 ttggtaaaaa agccaatgat tatttcttag aaatcattga atggattcca cgtcttatcc
     5821 atttacagct actatggttc ctgtgcgtct tgcctgtttt cacattagga actgcaagtc
     5881 gaacagttct gtatatgatt tatcattaca aaaaaaatga agagaaaaag ctcggttcac
     5941 tattctggaa aaagtttcgt gagaacttct ctctttatgg aaagcaagat agtttagcaa
     6001 gtgtttattt tcttcttcta ttgatcgatt atcagatttt ccgctattgg ggtgggggaa
     6061 ttggctatac attgatgtat actacgttgt ttttgcttct attaagcgcg cttctcttta
     6121 gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc ttggattact gcatttttct
     6181 tgttttttaa tgatttcaaa agcgcgcttt tctatttggc aggcacgttg atccttcttg
     6241 tcgcattctg gtttgcagga ccaatctatt ttgctttatt aggtttcagt ttcttgtttt
     6301 attttcaagt cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa aaggaggaat
     6361 aacatgttgc gtttgattgc taaaatcgat gatagtatta tcaacaagtt cgccaagcaa
     6421 cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta tcagtaatat ctctaatggt
     6481 ggttttattt ggttactcct tgctgctgtt ttagtcttga tacctgatac tagagaatat
     6541 ggtctagcca ttcttatcgg cagtttgatc tatttttttg ttggtttgct ttttatcaaa
     6601 aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct ttacacaagt
     6661 aaaccgaaga cttcttcttt tccttcagga catacagcgc tagccttttt atcagtagct
     6721 gcgttgttcg aattaagtcc agttgcagga atattagcct ttttatttgc ttgtttgatc
     6781 gcttattcga ggattttttt acttttacat tatcctactg acattttagg aggagctatt
     6841 ttaggaagtg ttattggcat cctatgcgct gccgttatct gaattaattg tacgaataaa
     6901 gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa agcgtagttg
     6961 tttattcatt tgtcttaata acttaagttt t
//
LOCUS       DADJYG010000124         6892 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.128, whole genome shotgun sequence.
ACCESSION   DADJYG010000124 DADJYG010000000
VERSION     DADJYG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.128"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            134..409
                     /gene="rpsP"
                     /locus_tag="JZ382_002130"
     CDS             134..409
                     /gene="rpsP"
                     /locus_tag="JZ382_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY2428299.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            421..672
                     /locus_tag="JZ382_002131"
     CDS             421..672
                     /locus_tag="JZ382_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAY2428300.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            797..1588
                     /locus_tag="JZ382_002132"
     CDS             797..1588
                     /locus_tag="JZ382_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428301.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1758..2279
                     /gene="rimM"
                     /locus_tag="JZ382_002133"
     CDS             1758..2279
                     /gene="rimM"
                     /locus_tag="JZ382_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY2428302.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2269..3030
                     /gene="trmD"
                     /locus_tag="JZ382_002134"
     CDS             2269..3030
                     /gene="trmD"
                     /locus_tag="JZ382_002134"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY2428303.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3166..3513
                     /gene="rplS"
                     /locus_tag="JZ382_002135"
     CDS             3166..3513
                     /gene="rplS"
                     /locus_tag="JZ382_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY2428304.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3645..4781)
                     /locus_tag="JZ382_002136"
     CDS             complement(3645..4781)
                     /locus_tag="JZ382_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428305.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(4897..5811)
                     /locus_tag="JZ382_002137"
     CDS             complement(4897..5811)
                     /locus_tag="JZ382_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAY2428306.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(5902..6330)
                     /locus_tag="JZ382_002138"
     CDS             complement(5902..6330)
                     /locus_tag="JZ382_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAY2428307.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(6335..6709)
                     /locus_tag="JZ382_002139"
     CDS             complement(6335..6709)
                     /locus_tag="JZ382_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428308.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         2198 a         1203 c         1251 g         2240 t
ORIGIN      
        1 aagaagtcta aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa
       61 aaaagcttta cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag
      121 gtggaaataa ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc
      181 gttttaccgt atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac
      241 tgtaggtact tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt
      301 tcttgactgg ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa
      361 agaaggcgtt atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat
      421 atgaaagatt taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat
      481 caagtcaagt tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct
      541 cctgaagata ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca
      601 atcgtttacg gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat
      661 aaggaaaagt aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt
      721 tattaattga agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg
      781 agggagtgaa cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag
      841 cgcttgtagt tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga
      901 aactgttacc agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat
      961 ggctacctca aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa
     1021 tgttcccatg cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca
     1081 tacaaagcaa ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc
     1141 tgacgaattg aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc
     1201 tagtcaagaa acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt
     1261 ggacttaggt tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga
     1321 atggcaagaa ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc
     1381 cttactatta gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga
     1441 acaatttggt aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag
     1501 ctggcaagaa ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa
     1561 gatggcagtt tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa
     1621 ctccaagtca caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta
     1681 ataccttaaa agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa
     1741 agaaatgggg atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag
     1801 gtatcaaagg cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa
     1861 aaggaacggt gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa
     1921 gtcatcgcaa acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg
     1981 atgtggaaaa atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag
     2041 ctgaaaatga attctattac catgagatca tcggtctgca tgtattagaa gatggaactg
     2101 agttaggtaa agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac
     2161 gtccgaaaaa agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt
     2221 tagaagctgg agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat
     2281 gtattgactt tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag
     2341 gctgtagata aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac
     2401 aaacatcaga cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc
     2461 cagcccatct atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga
     2521 gtaattcttc ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca
     2581 caagaagaac acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc
     2641 acattagtga cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt
     2701 gcgatggtca tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact
     2761 tcagctcaaa cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg
     2821 gcagaatata aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt
     2881 gaagaatggc aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg
     2941 gaaacacttg aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa
     3001 caagaaaaaa cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt
     3061 gataatatgt tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct
     3121 gtgagaaaag ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc
     3181 gaagaattaa caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact
     3241 gtgcgtgttc acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt
     3301 gttgttatca aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct
     3361 aacggagtgg gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa
     3421 gtagttcgct acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga
     3481 aaagctgctc gtatcaaaga aatccgtcgt taatcatcgt tatcagactg agaaagaacc
     3541 cttggtattc aagggttctt tttttgttct ctgttttttt atattaacga tttctatttg
     3601 ggacattttt gattaataaa aaggggcaaa aaaggggcaa caagttacag tttttctata
     3661 tcttctttca atttttcttt tactcccttt gtcacatgca aataaatctt ttcagtgata
     3721 tcactatttt cgtgcccaac gcgatcctga atagcataca gaggagttcc tagttccgct
     3781 aattttgaaa tatgggtatg tctaaaaata tgtgaactaa gttttttatc aatcttcatg
     3841 tcagctttat gatttctcaa ataggtgtta attgctgtta gttggaaagg agttcctttc
     3901 gttgtttgaa atagaaattg accattcgga tttaattcta gaagctcatt gtaaatagct
     3961 atcgcttttt ttggtaaatc aatttctcgc attccagcag cagtttttgt agaatcagat
     4021 tttttcatat cagctattga acgttctcta tacatcatcg tcccatttat aactactgat
     4081 gcattattat tggtaatgtg tacatcgtct ttacttaatg cgattgcttc tccaggcctc
     4141 attccagtca aatatagcca ctggaaaagg agagaatatc ttttattgtg tgaagttgtg
     4201 aaatttacta gtcttttata ttcatcatct tctaaaaact tatctttaat ttttatcgtt
     4261 tttgactctc gtttataatc aataactact tcgttgatag gatttttttc tacatagcct
     4321 tttttcatag cgtatgaaaa aagaagattc aatttggatt taattacact gacgtactta
     4381 tttgacaagt catctttata tatcatatct tcaaaaatat tatttaaatc tactgtatta
     4441 ataccagaaa cgatgtaagt ctcaggtatc tttttcttta tagtgtttaa gatattattc
     4501 gtaggataat atgtagactc cttaacttgt cttttgtaaa taactaacca ttcttcgaca
     4561 agctcatgaa atgttagatc aggcttctga agtgtttttt cgtttaattt tatatcaatt
     4621 tttttattta actctagcaa tgccactttc tgagtttctc gagatttatt tttataagta
     4681 atgctcactt tttttctttt tctagttttt ggatcaatat atcgctcatt atatttatat
     4741 actttttttc cgtttttatc agttttggtt tcaatccaca ttgttttcat ctcctatttg
     4801 ttgctataat aggaatggat aagtaagcca attatagcag gtttattttt catcacgtcc
     4861 acaaactttg gcgagggagg gggcgtgttt ttttgtttag tagctacctt tccatgaagt
     4921 atctgcgaag aacacttcaa atgttgttcc atcatttttc acttcaaaat atgctgttcc
     4981 ttgtgcggat ttacccggtt ggatggtttc cgaaaagtaa tctctactag ctaaatcaga
     5041 tttaatatct gatgaatcat aaaattctaa ataacttgaa cttgcatcaa aaggtgtagt
     5101 ccctgtgttt tctacaagaa aatctacctt tgcgtaaaat aggccttctg gtttatagaa
     5161 atcatcaccg taatcttttg atacagactg gacagttacg ttaatagagt taccattttc
     5221 agaagtaaaa ggaactgctt ctcctatact atgtgcaact gatgccgtct ggggttctga
     5281 agacgaagtt gtagttgtag ttgtagtaga gcttgaatct ttggattttc catctttatc
     5341 taagaaagtt cgttgattat aatatacaac ctgttcagtt tttggactaa cccaaattaa
     5401 cattgtttca tcatcagaat cagttgaata agtatattgg agcatctcta atttttttat
     5461 atcttttgca ctatttccat gttcaattaa ttcagagtgc tctttataat aatctccttg
     5521 tgcatccttt gaaatagata caattaattc atcccgtgtg atagcattgt ctaattcttt
     5581 gtaaacaaaa tcatttgtcc atttacgtaa cggtgaacca agtgtctgat aaacttgagt
     5641 taaatcatca ccaactttta ttttatgaac gtctgatgaa gttagttttt ttgtttcata
     5701 ttcagatgtt gatgaaacag tattttcttt agtgctagca gaattaggac tagtggagca
     5761 tccgcttaat accaagccta aaaaaagcaa cccaaaaaca atttttttca tttttaattc
     5821 ctcacttcta tgatatgatt tttttgtagg atcttagaaa taaggttttt agtccgtgtt
     5881 gcagcacgga ctttttttac attatataag aaagcgataa gcgctttctg gaagtccgta
     5941 aagattcttt aattcctcaa ttcgtttagg atattgatca ttgtcttctt tgtatagaga
     6001 aacaatgaga ttagcagcaa agcaattagc ttcgctttca gatttgcttc tagatgttct
     6061 tgttgataca tagtaactgg ataagccacg atgaaaaata gcgtgaccta attcgtgagc
     6121 gcaaatgtag aatctttcct cagagtctct cagttcatca tttaagaaga ttattgcacg
     6181 acctctaatt tcttgaaact gtcctttggg attctcgata aaaggaacgt attgaatttt
     6241 aatgcccatc ttttcacaaa tataaaaagg attagcggac tggtatttcc gcttcaactc
     6301 ctcgactaaa ttaatcgtat ccatctccat aagctcacat ctttttgcct ttttctttgt
     6361 cttctttcac aatatcccag aaggttccaa taaggatatc ttttacgcgt tggacctgtt
     6421 caggcgtcag tgtttctccg ccataagaca tattgacatt tgaatctagt aatttatcaa
     6481 gttcaactac ttcatctttt gttgcccagt caggaacttg gtttctaccg agtaaataat
     6541 cagtagttac attaaaataa tctgctacaa gttgtagtct tttagtacta ggagttctat
     6601 ttttccattg ataaatcgtg tttttaggta tatttaattc ttcttccaat tgagttatac
     6661 ttatgcctct tttatgagct aattccttta ttctatctag taaattcatt ttcgcacctc
     6721 acaagctacg aaaacactaa taaaaaaatt agttaaaggt gttgacaact aataaaagtg
     6781 ttagtatact gttttcgtaa gctaaaatat tagctaaaaa gacttgataa ctaataaaca
     6841 cttcacggtc ggcaaactct gaaatgtaaa ttactaggca tttctgtgtc tt
//
LOCUS       DADJYG010000125         6838 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.130, whole genome shotgun sequence.
ACCESSION   DADJYG010000125 DADJYG010000000
VERSION     DADJYG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.130"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..578)
                     /locus_tag="JZ382_002140"
     CDS             complement(<1..578)
                     /locus_tag="JZ382_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAY2428309.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQT"
     gene            complement(578..1714)
                     /locus_tag="JZ382_002141"
     CDS             complement(578..1714)
                     /locus_tag="JZ382_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY2428310.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1746..3197)
                     /locus_tag="JZ382_002142"
     CDS             complement(1746..3197)
                     /locus_tag="JZ382_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAY2428311.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3241..4005)
                     /locus_tag="JZ382_002143"
     CDS             complement(3241..4005)
                     /locus_tag="JZ382_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAY2428312.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4033..4731)
                     /locus_tag="JZ382_002144"
     CDS             complement(4033..4731)
                     /locus_tag="JZ382_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAY2428313.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4743..5522)
                     /locus_tag="JZ382_002145"
     CDS             complement(4743..5522)
                     /locus_tag="JZ382_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAY2428314.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5538..6482)
                     /locus_tag="JZ382_002146"
     CDS             complement(5538..6482)
                     /locus_tag="JZ382_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2428315.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6528..>6838)
                     /locus_tag="JZ382_002147"
     CDS             complement(6528..>6838)
                     /locus_tag="JZ382_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428316.1"
                     /translation="KVTSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLD
                     QLFDTMLKDGQVTATATYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY
                     "
BASE COUNT         1892 a         1484 c         1052 g         2410 t
ORIGIN      
        1 aacgtctgac ttgaaattcc taatttcatt cgtacatcat ttctaatact ttcatcataa
       61 taaaattttt tagcatccac tgcatttgga atcaaagtga atttatcatt tcgaaacaaa
      121 aaatcacctg ctacttttcc acatgaaaat tcatgtgtca aaacagacgt taacttacat
      181 cgataatatt gctttaaaag atatttaaaa tctctatcta tcgatgtatt atggctatgt
      241 gctatccgta tcggaatccc agcttttttt gcagctttta aaggtaaata actcatcgca
      301 tcaatatgag aatgaacaat ctcatattct ggatgatcct taaaaaattg ttccatccat
      361 ttaaaatatt taggataatt ttgtggataa agtctcggtg cataatagac ctttcctcca
      421 agacttatta tttcctcatc ataatcactt ttatatggac gatgtgtcaa aaaatcaaac
      481 tgaattttag ttcgatctat attcctataa tagttcatta gcatcgtctc caaccctgca
      541 cgatgcatat cattcacaca ctgtaatatt cgaatcatta tatatttccc ctatacaaat
      601 ttaatgtttc ttgtacgatt ttatcccaat tatatttact acaaataaac tcagtagctt
      661 gattcttcaa aactttaacc atttccgatt gattgcatgc ctcttctaat cttatcttta
      721 aatcacttac atccgatttc ttaaaaatca tagccttgtc ttctactact tccgtacact
      781 ctgagatatt tgaaactata caacagttcc cataactcat tgcttctaac aaacttaatg
      841 gcattccttc taaatcactt ggaagtgtat agatatacgc attactatat aattcttcta
      901 attcctgtcc ttgtacaaat ccagtaaata taattcgctc atcacctttt gctaattcct
      961 ttagttcatt tgcaaattca tctgtatcag agcttcctcc ggctattata agttttttat
     1021 ctgtctgaac atctttaaat gcctcaatta gataccgaat tcctttttcg ggaaccaatc
     1081 gccccaaaaa caaaatataa gaatcttttg ttaatccata cttttctgta atcaatcctg
     1141 ctgattttat ttcaattttt ttaacgccat ttggtataaa ccttgttttt cttccatata
     1201 tatcttcaaa ataattttgg acgctttcac taagtacaat tatttcatct gcaaatttta
     1261 cagcacactt ttcccctaac atgatgtatg tactagcaag cttgttccat ttttctcttt
     1321 gatgatctag accatgaatt gttgcaacac atcttttccc aaaaagtttt ggcaaccaca
     1381 acatagcaca tggtccttct gcatgaaaat gaacaacatc atattttccg aatgctgctc
     1441 taattccacc aaatactgaa gaactcattg ctgcaattcc ttttatattt aatgtaaaaa
     1501 tagtctttaa ttttacacct ttatattctt tatttttatg ctggtcaaat tcctttccgc
     1561 taacatgatg accacttcga ttatagcaag tcacttcatg cccaagtgca accattcgcg
     1621 ttgccaattc ttctaccact atctcaattc caccttctct tgatggaatt cttttatgac
     1681 ctagcataca gatttttaat ttttttcttg ccatcacgtt ttactccttc tatagtatct
     1741 ttatcttaca tggaaccgtt tctatgaagc acagttttga ctgtcctaaa caaaattcta
     1801 aaatctaatc ccatactcca attcctgata tattctgcat caagttttac aacctcttcg
     1861 aagtctgtga tatcacttct accactcacc tgccacattc cagtaatccc aggtttcatc
     1921 gcaattctcg cacgatgatg aaaatcatac ttctcatatt ctgacttcaa gcaaggtcta
     1981 aaccccacca ggctcatttt tcccattagg acattataaa attgtggaaa ctcatccaat
     2041 gatgtagatc gaataaattg tccaattcct gtcttctttg ttccatcagg aagtatttta
     2101 ttgccaatga ttcttgggtc aaaatccatc ttaaacatga gattattact catcttattt
     2161 tctttcatca actctgcttt gcgttcttct gcatccatat acatactacg gaacttatac
     2221 atcttaaatg tcttaccatt ttgaccaata cgttcttgtg caaagaaaat cggccctggt
     2281 gatttgatgt aaataactgg tgctacgaag ataaaaataa cacctgttgc aatacatcca
     2341 ataattccag caagaatgtc aatcgttctc tttataaatg cttgcctcat cgtcataaca
     2401 ttaatactag atgtaataac agtataatca ccaactcggc caataaattg ttttccacgt
     2461 gtactatcag acacttttgt aagattcata tgtacagcaa gtcccatttc cgaacatctg
     2521 gcgattaatt ctttgggata tggaacactt tcgtcaacat tgatgaatac ttcatctacc
     2581 caattctgta aaagatattc cgctgcatta tcagcatttg caactactgg gacacctccg
     2641 atggtagttc catacatatc tctgtctact acaatcacac cactaagttt atacatgcca
     2701 taattcttct ctttaagatt acgaactaca ttttttgcaa tcttttgaga tgttacaata
     2761 actaacgata catcttcacc gtttttcatg cggtgatgca acaacccttt ccaaagtata
     2821 cgaattatat atgttaataa tccatacacc acccccatta taaataaaga aactctggaa
     2881 taatcatttc catcctgcat tgtaataagg tataaactgc acacaagtaa cagcataaaa
     2941 gattgcttta tagaagctac agcctcttta taatatcctc tttttaaaac atttttatat
     3001 gcctcaaata aaaatataat cgtaagattc tcaagttcta taaaaattgc catgtggcga
     3061 taaataggaa tcacatacgg attccagtca ccctgtcgca ttacatatga tatataaaaa
     3121 gcaagctgga cacaaaataa gtctaataaa ataaaatcaa tatgtttaga ccaaccactt
     3181 gagttttttc tatacatctc tcactctctc ctcatttcct ttttatctac gaataaagaa
     3241 ctaaaataaa ccaaacttct tcttttttac ctcttcatag attggataat tgatctcatc
     3301 cccgttgatc agatcctttg cgacttgttt catctgtact actttttcct taccaaactc
     3361 cttggcaatc tgctcatagc actcttttaa gtaaaaggtg cgcttagaga ccccgtgtgc
     3421 atcagaagca gccatctgca cgagattatg tttgaccatt gttttggctg ttttctggat
     3481 atctttgcca aatacaccaa cataacttgg cgcagtaagt tgggccaaac atcccatatc
     3541 taggaacgga agcaaatgat ttgggtcttc tcgaaaatgc gcgttgcgtt ctggatggac
     3601 gatcactggt atctttccta gttctaacaa ctcaaaaaaa agccgttctg catataacgg
     3661 cacttccagt gtcggaaatt cgatcagaag atatgtatca tctaaatctg taaataaaat
     3721 ttcatctcgg tggatgtctt cgatgagcgt tccggtaata cgtacttctt gtccttccaa
     3781 taacgtcaaa ggcaactgtc ttttctctaa ttcggcttgt aacgaagcga ctagagaaat
     3841 gacttgtgat ttttcattgc tgtattttcc attattgtgg tgtggtgtgc ataaaatatg
     3901 cgtaatgcct tgctgaatcg ctttttcagc cattgcaata cttgcttcta agttttctgc
     3961 cccgtcatcg attcctggta ggatatggca atgtagatcg atcatttctc ttgtctcctc
     4021 tcttcttatt ccttaatttc cgtagtaata accagcatct ttgctgtgtt ctgctccgtt
     4081 gtatacgaca cctaagactc gtgcttgtac catatctaac agttcttttg ctttagtcaa
     4141 tgattctttt cgtgacgtat tttcccgtac gaccaataag gtgccatccg tttttgaaga
     4201 catgatctgt gcatctgtta cggcaacgac tggcggcatg tcgaagatca ctacatcaaa
     4261 tagttgtctt gcttctgcta aaatctgatc cattcttggc gaacttagta gttctgatgg
     4321 atttggtggt ttaggtccac ttggcagaat cgacaaattg tcgactggtg ttcgttggat
     4381 ctcatctgct acacttcctg atgaactaag aacagtactt aatccgcttg cgttgttcaa
     4441 cttgaacgtt ttatagatga cgggcttacg taagtctgca tcgactagca gcacccgttg
     4501 accagatttg gcaaaaacaa cggcaatgtt tgctgcagtg gtcgatttcc cttctccagg
     4561 tcctgatgaa gtcacaacaa tcgttttgat ttgctgacca gctgcggagg caaactggat
     4621 attcgtccga atcgtacgat attgttcagc gattggtgaa gagggattgg tgagtgtgac
     4681 caaactgact gcatgagttt gggctgcttt ttgtttctgt gttcgtgcca ttttctttcc
     4741 tcctagactc ttgagcggct tctgcgtgat ggcgctgttt tatcgtttgt agcttttggt
     4801 tgtgtctgag ctggtccttt tttacggata ccagcattca attctttcgc tgacatttct
     4861 ggaactgttc ctaagattgg gaatcctaat gtatctgtca catatttttc atctttcact
     4921 gtccgatcga gtaattccaa taagaatgcc agacctacac ctaccatcat tcctaacatt
     4981 aagccagtca ttaaattcaa tttattattt ggcgaaacag gtgtcgtatt ggcaactgca
     5041 cctgaaatga tcgagatttt atcaacattc atcacatctt ttgcgttctc ttggaaaact
     5101 tgtgaagttg tgttagcgat ctgttgagca gccactgcat tcgtacttat tgcctgaatc
     5161 gagaacattt gtgaattttc tgcctgatta actgagatgg catctttgat ttgttcagca
     5221 gtcattttca cattgtcttc tgtttctaaa cgatctttga cttcattgag cactaaatca
     5281 ctgacgatca tatctttata cgtattgatc atttgcaggt tcgtattgac atcattgaca
     5341 tttgcagtgt ctgattgtgg cagtgtcacg atcaattgtg cttgtgaact gtattttggt
     5401 gtcaagatga aaaatgttac gattcctgag atggccaagc cagcaaacat tgagatgatg
     5461 attattacca agtgtttgcg taatgtttca aaaatttctt tcaagctgat tgtctcttcc
     5521 attttttctc cactttccta tttattctct agattcattt gatcttttaa ttcttttttc
     5581 acacgatcaa gttcttgatc actgactttt tgataggaga tattatcttg catgaagcct
     5641 tctccttgca gctgatcttg cttaatcgta ccaaatgcct cccgataatc taaagcaatt
     5701 tttttcattt catcaaagtt catgtctgtt ttcatattcg actctagagc attcaaaacg
     5761 gattgatagt tgctgacacc gtttagactc aatacttttt tcgctacgcc ttctacgatc
     5821 tttctttggc gcccttgtct accgtaatca ccatttgggt cttcataacg catacgagaa
     5881 taagataatg cctgatcccc gttcaatgta attttaccga ttggaaagtc atagccatct
     5941 tgtgagaaag tgaggtcatt attgacttcg ataccaccaa ctgcatcaac gagttccttt
     6001 agccctttca tattgataga gacatagtga tcgattggga tgtctacata tttttgtacg
     6061 gtatccatcg ccatggcagc tccgccgaat gcgtaagcgt ggttgatctt gtcttttgtg
     6121 tcacgaccta cgatgtcgac atacgtatca cgaggaatgc tgacgatcgt tgtctgtttg
     6181 tcttcaggac tgactgtggc aaccatcatc gtatctgatc ttccttgatc gacccgacct
     6241 aaatctccag tatcaatccc catcaataga acagagaaag attcttgttt gtccagatca
     6301 actggtttcg ttcgttctcc ttgggttcgt tccactgatt cgtaagtttt ctcaatagaa
     6361 tgtgagagat ccagatataa ttttgctcct actcctacaa ttgctaatac taatacgata
     6421 agaactgcta aaataatcag aaatattttt ttccctcttg attttcttct tcgtcttgcc
     6481 atagtttcct ccgtttgcta accttgcttg aatcaacgtt ttaactgtca atagcttata
     6541 ttgctatagc ccatttgctc agcaacagtt tgtgcttttg ttgcgtatgc tgcgcttatt
     6601 ttttcattcc ggatctgctc tatttgactg agcaccgtca aatattgatc gtaagtagcg
     6661 gtggcagtca cttgaccatc ctttaacatc gtatcgaata actgatctag ttgcgtgact
     6721 tgcgcgatct gaatatcagc gaatcctaga tattgcataa caaccgtttt ccaaggacta
     6781 tctttaaaaa agctcatatt ttcacgtaca cgagcgacat ctgcacttgt cacctttt
//
LOCUS       DADJYG010000126         6739 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.131, whole genome shotgun sequence.
ACCESSION   DADJYG010000126 DADJYG010000000
VERSION     DADJYG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.131"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(65..523)
                     /locus_tag="JZ382_002148"
     CDS             complement(65..523)
                     /locus_tag="JZ382_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY2428317.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(536..1861)
                     /locus_tag="JZ382_002149"
     CDS             complement(536..1861)
                     /locus_tag="JZ382_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAY2428318.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1878..3290)
                     /locus_tag="JZ382_002150"
     CDS             complement(1878..3290)
                     /locus_tag="JZ382_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428319.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(3307..4071)
                     /locus_tag="JZ382_002151"
     CDS             complement(3307..4071)
                     /locus_tag="JZ382_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAY2428320.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4175..5050)
                     /locus_tag="JZ382_002152"
     CDS             complement(4175..5050)
                     /locus_tag="JZ382_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2428321.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5305..5389)
                     /locus_tag="JZ382_002153"
     tRNA            complement(5305..5389)
                     /locus_tag="JZ382_002153"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5353..5355),aa:Leu,seq:caa)
     gene            complement(5407..5478)
                     /locus_tag="JZ382_002154"
     tRNA            complement(5407..5478)
                     /locus_tag="JZ382_002154"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5444..5446),aa:Gln,seq:ttg)
     gene            complement(5488..5560)
                     /locus_tag="JZ382_002155"
     tRNA            complement(5488..5560)
                     /locus_tag="JZ382_002155"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5525..5527),aa:His,seq:gtg)
     gene            complement(5622..5706)
                     /locus_tag="JZ382_002156"
     tRNA            complement(5622..5706)
                     /locus_tag="JZ382_002156"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5670..5672),aa:Leu,seq:caa)
     gene            complement(5727..5797)
                     /locus_tag="JZ382_002157"
     tRNA            complement(5727..5797)
                     /locus_tag="JZ382_002157"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Cys,seq:gca)
     gene            complement(5843..5914)
                     /locus_tag="JZ382_002158"
     tRNA            complement(5843..5914)
                     /locus_tag="JZ382_002158"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5880..5882),aa:Gln,seq:ttg)
     gene            complement(5926..5998)
                     /locus_tag="JZ382_002159"
     tRNA            complement(5926..5998)
                     /locus_tag="JZ382_002159"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5963..5965),aa:His,seq:gtg)
     gene            complement(6001..6074)
                     /locus_tag="JZ382_002160"
     tRNA            complement(6001..6074)
                     /locus_tag="JZ382_002160"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6040..6042),aa:Trp,seq:cca)
     gene            complement(6081..6161)
                     /locus_tag="JZ382_002161"
     tRNA            complement(6081..6161)
                     /locus_tag="JZ382_002161"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6125..6127),aa:Tyr,seq:gta)
     gene            complement(6163..6238)
                     /locus_tag="JZ382_002162"
     tRNA            complement(6163..6238)
                     /locus_tag="JZ382_002162"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6203..6205),aa:Phe,seq:gaa)
     gene            complement(6274..6346)
                     /locus_tag="JZ382_002163"
     tRNA            complement(6274..6346)
                     /locus_tag="JZ382_002163"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6311..6313),aa:Asp,seq:gtc)
     gene            complement(6356..6428)
                     /locus_tag="JZ382_002164"
     tRNA            complement(6356..6428)
                     /locus_tag="JZ382_002164"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6393..6395),aa:Val,seq:tac)
     gene            complement(6444..6515)
                     /locus_tag="JZ382_002165"
     tRNA            complement(6444..6515)
                     /locus_tag="JZ382_002165"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6480..6482),aa:Glu,seq:ttc)
     gene            complement(6530..6619)
                     /locus_tag="JZ382_002166"
     tRNA            complement(6530..6619)
                     /locus_tag="JZ382_002166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6583..6585),aa:Ser,seq:gga)
     gene            complement(6659..6732)
                     /locus_tag="JZ382_002167"
     tRNA            complement(6659..6732)
                     /locus_tag="JZ382_002167"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6696..6698),aa:Asn,seq:gtt)
BASE COUNT         2026 a         1493 c         1100 g         2120 t
ORIGIN      
        1 atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact tttttcatcg
       61 tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt tttaataaat
      121 ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt gcttgctcca
      181 acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg ttactttcaa
      241 cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata tggatcagta
      301 tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat atattatcga
      361 ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg tatccaactc
      421 ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata atttgaccac
      481 tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat cacctctact
      541 ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt ttttttgcga
      601 ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt tgataagaac
      661 cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc atcaatcctt
      721 tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt gcaccaacat
      781 atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct tgattaggaa
      841 cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata tcgacgatat
      901 attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg cggcgtattt
      961 tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta gcacgtgtat
     1021 actctttgtc agtatgctca acgaccatat caggatataa gtaatactct aagtaattat
     1081 tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag gttttttgcc
     1141 aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt agtttttcaa
     1201 taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta taccagccaa
     1261 agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg taattcactg
     1321 caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt acattcggat
     1381 atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc aaaatccatg
     1441 ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg attgagcgca
     1501 tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca tgtttaagtg
     1561 gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt gagaaaacaa
     1621 aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga atatttgaaa
     1681 aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt ctttcttcat
     1741 caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc aaaatggctt
     1801 gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc tgttttttca
     1861 tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat aacataagtc
     1921 acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat cccgcttatc
     1981 cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc tacccgtagg
     2041 cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc ttgtagcatt
     2101 tttgttgcca tatattcata atttttactt gctaacccta actctgtttc ttctaaggtt
     2161 acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt tctcttattg
     2221 atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat tggaagaatc
     2281 aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca tgctccgaat
     2341 gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc ggcaaaaagt
     2401 gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac aggctcagtg
     2461 atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac catctcttta
     2521 ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc aaacatcatt
     2581 actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat aaagcgtgtc
     2641 atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc gtaataaata
     2701 tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa aaacataaat
     2761 atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc aatcccaatt
     2821 tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag aatcatcacc
     2881 atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa tttttgttct
     2941 ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc aaaaatcccc
     3001 atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat cgctaaaaaa
     3061 ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc aactcctata
     3121 gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat tactgggatg
     3181 ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat cgctgcaact
     3241 ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat ttttattcct
     3301 cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt tcactttttg
     3361 agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca caaaacttga
     3421 accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga tcgttttatc
     3481 ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat tgatttgtcg
     3541 ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat cgacatgacg
     3601 aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt ttgctaatgt
     3661 aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt ctaaatgagt
     3721 tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct gatacagttg
     3781 atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact ctccgctttc
     3841 gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta aatggactcc
     3901 tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac ccggcatatt
     3961 tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa tcccataatt
     4021 cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca tttcattctc
     4081 cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta ctatgtgtca
     4141 tttggttgcg cctatagata gttacgccct tattttaata gattcttgct agatgaattg
     4201 ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg atgatatctt
     4261 taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta aaaaaaccaa
     4321 tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat ttgatatgaa
     4381 ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag gatttttcta
     4441 gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa ctattaggct
     4501 ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt gaccctaact
     4561 cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca tattcatttg
     4621 gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct tctctttttc
     4681 gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa tctaatggag
     4741 aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata aattcgattg
     4801 gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct aatacaaata
     4861 atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag ccaatccctt
     4921 tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta tgattcagct
     4981 ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa caacttgcag
     5041 actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac gacctaagaa
     5101 agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat ttacaaaaat
     5161 agtttaacac ataataaaag cgttatcaat atctttttta ctcgttttat gataatactt
     5221 gtatcgaatt gatttggagg taattacata aaaacaaaaa aaatatagca actctctata
     5281 agaaagttgc tatattgata attataccgg cggccggggt cgaaccggca cgtcctcaac
     5341 ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa
     5401 taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta
     5461 ccgcttggct ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg
     5521 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt
     5581 aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga
     5641 accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac
     5701 gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt
     5761 tttgcagacc tctgccttac cacttggcta taccgccatt atactattat caatctataa
     5821 aataaagaat aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc
     5881 aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa
     5941 tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca
     6001 tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt
     6061 taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg
     6121 gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa
     6181 tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat
     6241 attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga
     6301 tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag
     6361 gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct
     6421 aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg
     6481 aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag
     6541 ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca tgctccttcg
     6601 gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa aatgagaact
     6661 ccggcagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
     6721 agctatgccg gaataacat
//
LOCUS       DADJYG010000127         6713 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.132, whole genome shotgun sequence.
ACCESSION   DADJYG010000127 DADJYG010000000
VERSION     DADJYG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.132"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..1062
                     /locus_tag="JZ382_002168"
     CDS             82..1062
                     /locus_tag="JZ382_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2428322.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            1184..2602
                     /locus_tag="JZ382_002169"
     CDS             1184..2602
                     /locus_tag="JZ382_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAY2428323.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            2673..4142
                     /locus_tag="JZ382_002170"
     CDS             2673..4142
                     /locus_tag="JZ382_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428324.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            4152..4619
                     /locus_tag="JZ382_002171"
     CDS             4152..4619
                     /locus_tag="JZ382_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY2428325.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            4655..5527
                     /locus_tag="JZ382_002172"
     CDS             4655..5527
                     /locus_tag="JZ382_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2428326.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            5608..>6713
                     /locus_tag="JZ382_002173"
     CDS             5608..>6713
                     /locus_tag="JZ382_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY2428327.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEES"
BASE COUNT         2294 a          945 c         1374 g         2100 t
ORIGIN      
        1 ggggaatgag agagtcaagg ttcttggaat gtcgaatagt cgagtaggta gattttctat
       61 ctacaacctt gttgtttaaa aatgaaaaaa attggaatta gagaagttgc gaaagaggca
      121 aatgtttctc ctacgactgt gtcgagggta ttgaatgatc gtggatattt gagtgaggaa
      181 acaaaaagaa aagtttttga tgccatgaaa aaattagaat actatcctaa tgaactggcg
      241 agagcacttt ttaaaaacga aacatatacc gtaggtttga tttttcctac aattactaat
      301 ccatttcacg ctgagttaat tcaagatatc gagttcgagc tttccagcag gggatataaa
      361 gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa aagagtactt gacgatgttg
      421 agaaagaatc aggttgatgg aattatagta ggaacgcaca ataaagatat tgcggattat
      481 gatattccag ggctacctat tgtagcgatt gatagaagtc ttggtgaaaa tacagttaca
      541 gtatcttgtg ataattacag gggtggggaa ttagctactc aactacttgt aaatcatggc
      601 tgtcagaata tactgtgtat tcggggagat agtaagataa agctacccgc gaatagtagg
      661 actttagcat ataaagatgt tttgaataaa ttcatgctgg aaccgttagt cctagaagta
      721 ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta agacatatct ggaaactcat
      781 ccaaatattg acggggtttt tgcaggagat gatttattgg cttctttggc gattaacaac
      841 ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag tgattggatt tgatggagct
      901 caacaaacat tgatttataa tccagaatta tcaactattc aacagcctat agatcaaata
      961 tctaaagtag cagtaaaaaa attattgaat atgattaagg gcgaaaagga gacggatgaa
     1021 ctttcacttc cagtaaagct cataaaaaaa aatacagcat aagttaaatc ggcgaaaaat
     1081 tccttcgtcg atttaaaaat atttttatga aacgggttga catatgaaac gggttgacat
     1141 atattagttg tgaagataaa aaccttttca ctggaggaag aaaatggaga aattattaat
     1201 taaaagcaat aatcatcaac tttactacca acctaaggat gtttgggtcg gagatgttat
     1261 gccttacggg aaagaaggaa aattctttat ttatcatcaa cgtgatacta gaaatccagt
     1321 tccttttgga gaaccgtttg gctgggcttt agcaactacg aaagattttg ttcattttga
     1381 agactttggg gaatctcttt taagagggga tgacaatgag agagaccagt ttatctacgc
     1441 tggttctgta tttgaggcaa atggaaaggt tcacgcattt tatacagggt ataatcgtga
     1501 attcttagcg cagggaaaga catctcaagt gttgcttcat gctgagagtg atgattttgt
     1561 tcattgggaa aaatcagaaa cagcattgga gttagaacca caagaagggt atgatattcg
     1621 aaactggaga gatccatttg tcgtttggga tgatgaaaaa gaagaatatt tgctaatttt
     1681 aggggcgcga aagggtcagg ataaacatct acaaaccgga cgtttagtgg aatttacatc
     1741 aaaagattta actaattggg aattcaaagg tgatttttgg gctccgggat tgtataccat
     1801 gtttgaaatg ccagaacttt tcaagattgg tgactggtgg tatcttgtct attcagaata
     1861 ctcagatgga aataaaatac tctatcgaat gagtcaaagt ttggaaggtc cttggattaa
     1921 gcctaaagat gaagcttttg atggacgggc ttattatgca ggtagaacag cttatgatgg
     1981 aaaacgtcga gtcctatttg gatgggtacc aacaaaagat caagaaaatg ataaaaacaa
     2041 ttatttatgg ggaggtactt tggttcctca tgaagtctat caaaaagagg atttttcact
     2101 tggggtaaaa cccatagacg aagtgtggaa tgcattcata aatttacatg atattgccga
     2161 tgtagagata gaaacagcag acacaagaga agaagtaacg cttgtcaaaa atagtggaac
     2221 tttgtttaat ttagaaacaa caatttcatt tgatgagaat cttagtgact tttcactgag
     2281 attatataaa aaccttgaga cagatgaatc ttacgaatat cgcttcaatt tagatgaggg
     2341 gcgtcttaca tttgataaaa acccttgtta cccttggttt caatatatgg ataaaggact
     2401 tattagacct atatctttaa agcctaacca atcttataaa ctaaaagtga ttgttgatga
     2461 caatataatg acactttata ttgatggaat cgctcttaac gctcgtgtac atggttgttt
     2521 tggtcaagca attactatta cagcaacaaa tggatcaatg actgcaataa ataccaagtt
     2581 ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg caggtctgaa aatcaaacat
     2641 gacctgtcac atttaaaagg gaggataaat gaatggacta caaaaaaatt gccgaagatg
     2701 tattggaaaa cgttggagga aaagagaata tctcaagcgc tacacactgc attactcgtt
     2761 tacgtttaat tcttaaagac aaaagcaaag cgaatatgga aatgcttgag gaaatagaag
     2821 gtgcaaaagg tgttcttttc aatagtggac agttacaagt aattttcgga acaggtataa
     2881 ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc taaggaagta tccgtttctg
     2941 aaatgaaaga cgaaggagtc aataattcag gaaatagact tcaacgagct tttaaagttt
     3001 tctcggatat ctttattccg atcattccag cttttattgg ggcagcaatg gttttaggaa
     3061 ttcgttcgtt gattaccact cctggaatgt ttggaatgga tggctcattc gctgatcaca
     3121 atgtattcct aaatgattta tccaaatttc tgggtattat tgcaaccaca tttgattatt
     3181 taccagtttt agttatgtat tctgcaacaa aacgttttgg cggaaaccct attttaggat
     3241 tacttgtagg atttgttatg attcatccag gactagccaa tcggaatgat ttcgtcatgg
     3301 gaactataaa gcctgattat tggcatttat ttggattaag cattccagca gttgcgtttc
     3361 aagggggtgt ttttccagca attttgactt cttggttttt agcaaaagtt gagaaaatta
     3421 caaataaata ttctcctcaa gtacttgctt acattttagt cccaactgtg acaattgttc
     3481 ttgccaactt tgcactattt ttagtgtttg gtccattggg gaattttatc ggtaatgttt
     3541 taggtggaat cattgatttc ctatatatga agatgggtgc ctttggtgct tttgtttttg
     3601 caggcgcgtt acaaccatta gttgtaacag gaacacaaca tgctattcaa ggtattgagg
     3661 caaacttagt cgcaacaact ggattcaatt atatccaacc tatttggtca gtttcaatta
     3721 ttgcccaagg tggcggttgt attggtatgt atttactctt taagaaaaaa tctaaagata
     3781 gagatattgc gatttcaagc tttattccaa cactggtagg tattactgaa ccggctatct
     3841 ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg ctcgtttatt ggtgctggtt
     3901 ttggtggagc atttatgaaa atttttgatg taaaagccat tggacaagga ttgactgtat
     3961 tgccaggtct gactattgct aatccagttc ttccttatat atttggtaat ctcatagcat
     4021 tcattttacc aatcattttt attctcgctt atgataaata taaagtgttt atccctgaag
     4081 ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa attgccagtt caagtaaagt
     4141 gagatgagag catgttaaat ttttttagaa aaaatgaaat aagcgttgta gcacctgcaa
     4201 ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt cttttcaaaa ggaatgatgg
     4261 gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt atctccaatc gatggttttg
     4321 tttcaagtat ttttcctaca aaacatgcct taggaataaa aacaaaatca ggaattgaga
     4381 tacttgttca tgtaggaata gatacagttg agctaaatgg tgagggattt gatattaaag
     4441 ttcaagaagg acaaaaagtt aaggctgggg atattcttat gaccattaat tttgtagttt
     4501 ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt tccagactac caagacgaaa
     4561 ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga attgtttgtt cttggctaat
     4621 gataagtaat taataataga atcgaggaaa atgtatggtt tttggggcta ttgaagctgg
     4681 tggaacaaaa tttgtttgtg ccattggaga taaagatttg aacatatttg aaaaagtcag
     4741 ctttcccacg actacaccca aagaaacaat gaatttagtt ttagattttt ttaatcatca
     4801 taaagatgat ttagaaggta tcggagtggg atcgttcggt cctattgata ttcattgtga
     4861 atctaaaacg tatggcttca ttacatcaac gccaaaactt gcttggcagg attttgattt
     4921 tattggaagt ttaaaaaagc aacttaatat tccaactgct tggacgactg atgtaaatgc
     4981 ctcttgctat ggtgaatatg tttctggaaa tggcagaggc ctttcaagtg ttgtttacta
     5041 taccgtagga acgggcatag gaggtggcgc tatccaaaac ggaaacttca ttgaaggttt
     5101 tagtcatcca gaaatgggac atatgcttgt aaaacgacac aataaagaca acttttctgg
     5161 gacatgtcca ttccacggag actgtttaga gggcttagct gctggtccgg caatcgaaag
     5221 aagatcaggg aaaaagggac aagaagtgac tgaaaatgac gttacttggg aaatagaagc
     5281 ttattacatt gctcagtgtg catataatac aacattgatg ttatcacctg atgtcattat
     5341 atttggtggc ggagtaatga aacaaaaaca cttaaaaaag aaagtacaag ataagtttat
     5401 tgagttgatc aacggatatg tcgctattcc cgatgttgac aattatatta tgacaccaaa
     5461 attgttagat aattctggta ttgtaggttg tttagcttta gcaagagacg ttaaagaaaa
     5521 atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg gttgatgcga tctttttttc
     5581 aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa tgaaagatga agtattagag
     5641 tatatcaaaa ataatttaaa gtattacgat ttttctgctc aagatattgc aatgaaattt
     5701 tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt tattttcaga tggaaaatta
     5761 ctaaagaatg attctgttag accagtaatg ttcaagtata atcaacaaaa gccaaaagac
     5821 tgcttttcta agtttattgg agcagacata tctttgaaat ccactataga taaatgcaaa
     5881 gctactgtaa tgtatccccc taatggcctc ccattaataa taaaaggaaa cagtggcgtt
     5941 ggaaaaagtt ttttagcaag tttgatctat cagtatgctc ttgatagaaa agtaattcat
     6001 aatgacgcaa aatttgttgt tgtgaattgt gcagattatg caaataatcc agagctattg
     6061 tcagcagttt tatttggata taaaaaagga gcctttactg gggctgaaaa agatactgca
     6121 gggatgctat cttctgctaa tggaggatac ctttttttag atgaggttca caatctaagt
     6181 gcagaaaacc aagagaaatt gtttcttctt atggattctc aaaagtatcg taaacttggt
     6241 gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct tagccacaac agaagataaa
     6301 aatacctctt tgttagcaac ttttagaaga cgcatcccag ctgaaatcac tctgccagat
     6361 tacaataata gaagtacatc tgaaaaaatt caattattat ttgaatttct taaagatgaa
     6421 gcaattacga ttgattcaaa aatattttgt tcgattgaac tttttactga tcttttatcg
     6481 aaaacatttg aaggaaatgt tggagaattg aggaatgaaa tcaagcttct ttgtgcagaa
     6541 ggttatttag gaaataagag aaagtcttca atttatttag gggacaaact agacagcggt
     6601 ttttggatag atccagaaat tagtatagat ttaaaaaaaa tgtttcttaa atcactttct
     6661 gaaattgatt tgattgaact ttttcaaaat cacatttcaa ttgaagaaag cat
//
LOCUS       DADJYG010000128         6706 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.133, whole genome shotgun sequence.
ACCESSION   DADJYG010000128 DADJYG010000000
VERSION     DADJYG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.133"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(7..1164)
                     /locus_tag="JZ382_002174"
     CDS             complement(7..1164)
                     /locus_tag="JZ382_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428328.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="JZ382_002175"
     CDS             complement(1167..1523)
                     /locus_tag="JZ382_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428329.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="JZ382_002176"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="JZ382_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAY2428330.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="JZ382_002177"
     CDS             complement(2991..3830)
                     /locus_tag="JZ382_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAY2428331.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="JZ382_002178"
     CDS             4015..4902
                     /locus_tag="JZ382_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAY2428332.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="JZ382_002179"
     CDS             complement(5486..6181)
                     /locus_tag="JZ382_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY2428333.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="JZ382_002180"
     CDS             complement(6165..6563)
                     /locus_tag="JZ382_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY2428334.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DADJYG010000129         6619 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.134, whole genome shotgun sequence.
ACCESSION   DADJYG010000129 DADJYG010000000
VERSION     DADJYG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.134"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            51..491
                     /locus_tag="JZ382_002181"
     CDS             51..491
                     /locus_tag="JZ382_002181"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY2428335.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            619..1302
                     /locus_tag="JZ382_002182"
     CDS             619..1302
                     /locus_tag="JZ382_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAY2428336.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1611..1684
                     /locus_tag="JZ382_002183"
     tRNA            1611..1684
                     /locus_tag="JZ382_002183"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1645..1647,aa:Arg,seq:tct)
     gene            1843..2202
                     /locus_tag="JZ382_002184"
     CDS             1843..2202
                     /locus_tag="JZ382_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAY2428337.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2325..3956
                     /locus_tag="JZ382_002185"
     CDS             2325..3956
                     /locus_tag="JZ382_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAY2428338.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4018..4659)
                     /locus_tag="JZ382_002186"
     CDS             complement(4018..4659)
                     /locus_tag="JZ382_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY2428339.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4941..5249
                     /gene="rplU"
                     /locus_tag="JZ382_002187"
     CDS             4941..5249
                     /gene="rplU"
                     /locus_tag="JZ382_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY2428340.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5276..5620
                     /locus_tag="JZ382_002188"
     CDS             5276..5620
                     /locus_tag="JZ382_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAY2428341.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5633..5926
                     /gene="rpmA"
                     /locus_tag="JZ382_002189"
     CDS             5633..5926
                     /gene="rpmA"
                     /locus_tag="JZ382_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY2428342.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6019..>6619)
                     /locus_tag="JZ382_002190"
     CDS             complement(6019..>6619)
                     /locus_tag="JZ382_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428343.1"
                     /translation="LFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEILALTWDNIN
                     FKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLEQRKYLFQLG
                     FTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASMLYEAGADHK
                     EVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
BASE COUNT         2017 a         1102 c         1386 g         2114 t
ORIGIN      
        1 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
       61 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
      121 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
      181 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
      241 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
      301 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
      361 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
      421 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
      481 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
      541 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
      601 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
      661 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
      721 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
      781 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
      841 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
      901 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
      961 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
     1021 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
     1081 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
     1141 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
     1201 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
     1261 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
     1321 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
     1381 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
     1441 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
     1501 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
     1561 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
     1621 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
     1681 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
     1741 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
     1801 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
     1861 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
     1921 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
     1981 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
     2041 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
     2101 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
     2161 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
     2221 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
     2281 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
     2341 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
     2401 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
     2461 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
     2521 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
     2581 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
     2641 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
     2701 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
     2761 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
     2821 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
     2881 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
     2941 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
     3001 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
     3061 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
     3121 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
     3181 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
     3241 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
     3301 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
     3361 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
     3421 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
     3481 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
     3541 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
     3601 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
     3661 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
     3721 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
     3781 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
     3841 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
     3901 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
     3961 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
     4021 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
     4081 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
     4141 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
     4201 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
     4261 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
     4321 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
     4381 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
     4441 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
     4501 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
     4561 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
     4621 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
     4681 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
     4741 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
     4801 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
     4861 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
     4921 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
     4981 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
     5041 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
     5101 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
     5161 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
     5221 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
     5281 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
     5341 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
     5401 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
     5461 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
     5521 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
     5581 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
     5641 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
     5701 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
     5761 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
     5821 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
     5881 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaataaa taaactgctc
     5941 tatctcttgt gtagcaaggg atggagcttt tttgtaaatt atgggatttt ttttgaccat
     6001 acttttgacc atactttctt agaagttaat ataatttgtt agtttttgtg ttgtttcttc
     6061 ttttttgctg ttagtaatgt gtgtatatgt gtccatagta gtttttatat ttgcgtggcc
     6121 cattctttct tggacttctt tgtgatctgc gcctgcctca tatagcattg atgcatgtgt
     6181 atgtctaaaa ccatgcagac cgatataagg taagccggca cgctcacgat atatattgta
     6241 tctttctgcc actgactgat taattgtgaa attattttcg tcattggtaa acaaataatt
     6301 agctttagta aatcctaatt gaaataagta ctttctttgt tcgagcttcc aaaattttag
     6361 ttgctttata gttttttcat ctaaataaat tattcgattt gattttttgg ttttaggagt
     6421 ttcagatata taatatttat ctgaacgagc tacagttttt ttaatggcca tttcatttgt
     6481 tttaaaatta atattgtccc aagtgagtgc taatatttcg ccgattctac atccgctgaa
     6541 tgctaatagt ctaaagagcg tatagtctct gtttttaaaa tatggattct gttcttgaga
     6601 tacttcattt agaaataat
//
LOCUS       DADJYG010000130         6511 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.135, whole genome shotgun sequence.
ACCESSION   DADJYG010000130 DADJYG010000000
VERSION     DADJYG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.135"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(716..3865)
                     /locus_tag="JZ382_002191"
     CDS             complement(716..3865)
                     /locus_tag="JZ382_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAY2428344.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3878..5077)
                     /locus_tag="JZ382_002192"
     CDS             complement(3878..5077)
                     /locus_tag="JZ382_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY2428345.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5067..>6511)
                     /locus_tag="JZ382_002193"
     CDS             complement(5067..>6511)
                     /locus_tag="JZ382_002193"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAY2428346.1"
                     /translation="ESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPE
                     YLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVT
                     ITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVT
                     LDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHG
                     VDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLA
                     PKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMP
                     ANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAER
                     KDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEK
                     ELLEAISSLQTTPENEMWLQGALEVFKHEQ"
BASE COUNT         1802 a         1343 c         1072 g         2294 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttgggcact tgaatctcgg ctattttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cgtccttcac gaatgagctg attgggaaac tcttttgtta
     2041 accaatcagg ctctttcgtc atctctttga tggcccgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatt gcagatttcg ttggcctgcc atttcgaaaa tcaaattttg
     2161 tataggcatt atctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagc tgggtccaaa taactttctt ttttgacagg ttccatttga tcaatcatac
     2281 gattgatctg ttctgatgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc tagtgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaga
     2401 cagagccatc ttctagcgca ttcttaatgg tataggtatg caagacctct ccatactgat
     2461 catgggttgt acgtgccaat tgccctttgg cttgtttccg attctcagga aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgg cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttctt tgcatatttc aacgccgcat
     2701 ctagtttttg acgggttgta atgattacaa catttgcatt ggcatctgcc aacagggttt
     2761 cacttaattc tttggcactt cccgttccaa cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa attttgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg ttcgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgtct ttcctgaacc agtcgcatgc cacacatacc
     3001 ctgattgatg tttatttgcg gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gagggtttta ttatcttgat cttcactgac gattgtataa tccgcaatca
     3121 aacgatgagc atccgggata tttaacactt gtttacaaaa ctcatataag ttttctactt
     3181 tttcattgtc tttcgttcgc caactaaata aaaacttagg gtgcaagtct ttcggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aatgcctgat
     3361 aaacaccatc ttttgcagtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 ttaataaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcataa gtagagattc ctccaccaat gtcttgattg gaataaagga
     3541 taagtgagac agaacccaat tgcgaatctt cccgttcaat cgtgatccga gccatcccat
     3601 tttcaccttt taaccatttc gcagcatcaa atggggtttt cgttcgcagc agtaattccg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagataat tttttggcgt aaattgaccc aaaggtcttc ttctgatttt agatccggac
     3781 gatacgtcca ctgattatgc ccttctccaa gtacacgaat caactgttct tcgacttctg
     3841 cttcctcacg gtgactaatt ttcattcttc ttccctccta gacaaacatc ttttgtaaga
     3901 agcttttttt cgtttctttt agtaaatcta acttacgttg atgaagagcg atcgtatcat
     3961 ctagctgttt gaagaaagta ccgatttttt gttgttcatc ctttacagga accttaacac
     4021 tgatattctc aatatcagaa ctgttataag aaggcaatgc ccctacttgg gagtatcttg
     4081 gcaaatttat agtttcaaac aatattttac caaaatagat atcccaagta ttattaaata
     4141 aataagccat ggtattgtta tctattaaga aagggcttgt gaccaaacgt tttctattaa
     4201 gcattattgc tgcgccaact ttagcaaata taatcgctgg cacatctttt attggcttcc
     4261 attttttacg ctcaatttgc tcattagata cataatgatt tgcatttctc atttcatact
     4321 cattaccaat gttattcatg tcggaaactt taaaaaatgg agtaccttca gaaccacctt
     4381 gctcggcatc aggaaatcca attcccgatt gagttttacc taattctccc aacttacgct
     4441 cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg cacttttgct ccgttttcag
     4501 ggaacatttt ttgtaagaat ccttttttcg tttccttcag caaatctaac ttacgttgat
     4561 gaagagcgat agtgtcgtcg agttttttga agaatatgcc gattttttgt tgttctttta
     4621 aatcattggg aatatagaat actgtatctg aaactttttt ccagtctgaa cgaggcattt
     4681 ttgttccaga tgtatcattt gcagcctttt gatatctatc agcttgaata agagaataaa
     4741 taaagtccga gtcgctcttc ttagctctaa aaacccagaa atccccaatc gctacgcctg
     4801 taaaagttgc cttgagccaa ttttttaaat atggacgtaa ctttccatat agaatatcat
     4861 ttggtaaaaa ttcaatccct tttctattat caaatttctg gctcacatct ttattcagtc
     4921 taccttctcc tgccacgata tcttcaaatt caacttttgg aagtctactg gagtttgtac
     4981 ttttattaag tctaacaact aaatcaatta acttacgctc ttcccaatct tccgtaaatc
     5041 ctgggaaacg gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct
     5101 tgtaaccaca tttcgttttc aggtgttgtt tgcagggaag agatagcttc taaaagttct
     5161 ttttctagca cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata
     5221 tcaattggtt cttcttcttc gaaagtatct acatagcgtg ggatatttaa gttatagtca
     5281 ttttctttga tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca
     5341 gcgtaggttt cgaggatttt ttgaatgttt tcttccgata gtttattttg atttttccct
     5401 ttaacaaatt cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc
     5461 aacacaatca ccgtcgttgg aatcgatgtc ccaaagaaca agttcgcagg cataccgatc
     5521 acagcataaa tactgccatc ttctagtaat ttttgacgga tgaccccttc tgcagcgcca
     5581 cggaataaaa ctccgtgtgg taagacgatg gccatggtac ctgtttcctt caaatgatag
     5641 aacccatgaa gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccataacga
     5701 ttaaaccgag aatcatctaa gaaagtcgta tctgctgacc aattagccga ataaggtggg
     5761 ttcattacta ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta
     5821 tccccattgc ggagattcat ttcttcggcg tcgacaccat gtaaaatcaa gttcattttt
     5881 gctagattgt atgtcgttgt atttagttct tgcccatgat atttaacgtt atctgggtgt
     5941 gttaagtagt tacgtacgtt taacatcaat gaacctgaac ccatcgttgg atcaaaaaca
     6001 ctaaagaatc gtcgttcctt ttgatcgagc gttacgattt gcgccatcat atcagaaacc
     6061 atatgaggtg tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc
     6121 agaaactcat acgcatcccc gatcacgtcc ccatcgtgtt ctaaaacatc tacatcattc
     6181 aatttcttga tgacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt
     6241 tttgattgga gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat
     6301 tgcacaaagg ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct
     6361 aagacgttga aaagatactc tggtgcaatt gcatatccca aaatatcaac gattgtggca
     6421 atcagatctt cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg
     6481 cttactgtgt catattcctc taaggattca t
//
LOCUS       DADJYG010000131         6375 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.136, whole genome shotgun sequence.
ACCESSION   DADJYG010000131 DADJYG010000000
VERSION     DADJYG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.136"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..1283)
                     /locus_tag="JZ382_002194"
     CDS             complement(<1..1283)
                     /locus_tag="JZ382_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAY2428347.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
     gene            complement(1442..1882)
                     /locus_tag="JZ382_002195"
     CDS             complement(1442..1882)
                     /locus_tag="JZ382_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAY2428348.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2042..2548
                     /locus_tag="JZ382_002196"
     CDS             2042..2548
                     /locus_tag="JZ382_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY2428349.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2716..4629
                     /locus_tag="JZ382_002197"
     CDS             2716..4629
                     /locus_tag="JZ382_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAY2428350.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4634..5635
                     /locus_tag="JZ382_002198"
     CDS             4634..5635
                     /locus_tag="JZ382_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2428351.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5837..6043)
                     /locus_tag="JZ382_002199"
     CDS             complement(5837..6043)
                     /locus_tag="JZ382_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAY2428352.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2225 a         1037 c         1306 g         1807 t
ORIGIN      
        1 ttcttagttt tcttgaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
       61 aagtttctaa agccataggc aattcgtttt agtactttga tatggttgtt caaacattct
      121 atagggccgt tggaataggg aactcgcaac gctcgtttga tttctgttcg atatttcttg
      181 aaggtcgtga acactggttg atagtgctct ggcaactcct tgtagtcttc ttttaggagc
      241 gcgacgaatt caggaacatc ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg
      301 tagcctcgct ttaaggagtc atcataatat aatagccgat cgactatctc cgcttctgtc
      361 agatgatctc gaaatgacgg acgccagtag cgatgctgac cattcaattc ccacgcattc
      421 ttttgaagta gtctccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
      481 gtcttcatta cttgaattcg gtggttggta aaggcccgac cgatgtgttg gctaatatga
      541 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
      601 gtatagaaat ctgaaaccac tagacgtact tgttcacgga cttttctagg gtagcgcaag
      661 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
      721 ttgccgtcca tcatgacaaa gctcatggct cccgagacgt ttttcaccga tttgaattca
      781 tcgaaacaca ccacttctgg taacggctgg ttcaaagaaa cggttttagg ccgaaggctg
      841 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcaat caaagacata
      901 gaaataggct cacttaatag ctcaagaatg gcttgtttga ctctgcgggc aatggagcag
      961 cgacgatcgc taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     1021 cgctgtttgt taatccgcac aatggtcttg tagttactga catcattcaa taagatggat
     1081 acttttttcc agccccattt aatgatcgtt tgcttatttt gatagtggca atggaagcat
     1141 tcctctggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc
     1201 tctttgcgaa aggcatcttc atgaaaggta agatttaggt ctagaatatc tagtaactct
     1261 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     1321 ttctaaagga tttcaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     1381 cagaaggcgt caccaacact agatattata gatccagaaa agcagattgt ttatgccaaa
     1441 attaagacat tgcaaaaaga ttattttctt taaaatcgac aagatgacca gaaagcaaga
     1501 gttcgttgag ctcttgcttt cttttagttt tagaaaggaa catcctgatt ttatcttcgc
     1561 taaatccagt caaaagtttt gcagattggt cgtagataat cggttttttc aataaagaag
     1621 gggatgtacg aatctcaata tatagttcac tcaaactgag ttcatctatt ctgtgaacaa
     1681 gatttttata actaatagaa tgagtagaga gcatttcggt cacgccgttg tttgtcaatt
     1741 ttagtaagtg gataaattca tttttggtta gtggttgacg aattaagttc tgttcgataa
     1801 acggtatatc atggacgata agccaacgtt ttgcgcgtct tgaagacgag cagccagaac
     1861 ctgtatacaa tttaatcatc atacattcct ccaatagcta agtatttatg aaataataac
     1921 atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa taagaaaatg ttgtcacgta
     1981 taatggaact agctagataa gaaatactat taaatcaaga aaaaagaaag aaggcacaag
     2041 tatgtcaaac acagccgacg caatagtagt gatcgattta caaaaagggg tatgttttga
     2101 ttcttcgccg atacacgaat tagaaacagt gacagcatta gtgaataaaa gaatcaaaac
     2161 atacgaaacg attgggaaac cagttatttt cgttcaacac gaagatgctc aattagtgcc
     2221 acaaacaaaa ccatgggagc tattgtcgga attatctatt cctgagcaca gttattttgt
     2281 gagaaaaacc catgcgaatt cattttataa gactaatttg agtgttttat taactgaatt
     2341 ggactgtcat tcgatcgaat tatgtggggc acaagcagag tattgtatag atacgactgt
     2401 gaagtttgct catggattag gataccagct tatgatgtgt agaggagcaa cgacaactta
     2461 tgataatcct tttatgaaag caaaggaaac aatcgctttt tacgagaaaa tttgggataa
     2521 acgatttctg acatttttgg agcaataagt tgtttcttcg tttaaaaaaa gcagaatgga
     2581 tactggttta tccattctgc tttattacgt aaaatgagaa aataatcctg ttctattttt
     2641 atgggataaa ccatttattt tttgtataaa aaatgtttgc gtttacatat aattctccta
     2701 gaaacataaa aaggagtgaa tagcttgaga acaaaacaag tattaaaggg agcgttggtc
     2761 atcttatttg ctgtaagtac aagcgtcccg caatggacag tatatggaga gagcatttct
     2821 tccgaatcgg aatctcggga ggtagaaacc acagaaacta ctgatgctgg aaatgaaacg
     2881 aaatcgagta cttcacaaac agaaacaaat actgaaacaa cggttgagac atctgttact
     2941 agcacagatc aagaaacgac cgctaccaca gataaccaac aaacagaaga agcaacagct
     3001 acaaaaatgt caaacgacga atcctcttct gctaaagtaa gtgacaaaga gaatgggaca
     3061 gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta aagaatattt gaattacttc
     3121 ggtaaacaga tgacagaaat acttaatgga ccaacagaag acatcaagtc aaaattaata
     3181 gagttattag tcactgagct tgctgttaat gattatggtg tagataaaga gattatcaaa
     3241 catctgattg ctacttatct attgtacgat atcgcttttg caaaaaacag tgtgttgatg
     3301 tcgatgcttg gaggtacaga tgatgggaca acgcctacag aaaacacagg cttctatcca
     3361 aatacaaaac aacttcatca aactgtacta aacaaacaag atggcaaaat gatggatgta
     3421 tatgcatatt atgtcgatca aggttcagat aaaacagtca tgatccatgg cggatttcga
     3481 gggaattgga ataacgggat cgttacagaa gaatacaatg atttttataa ggccgggtat
     3541 aatttattat ttgttgattc cagagcgact ggtaatagcg gaggcgatta tgtgacctat
     3601 ggacaatatg agtcagatga cgtcctttac tggataaatc aagaagttcg agagcggcca
     3661 tctcaaaaga ttttacttta tggtggttcg atgggagcag ctacgatgat gtctgtatta
     3721 gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa attgcggatt tgcatcaatt
     3781 gatgaacaac tacgttttac gtacagccag acagtagccc ccgcactacc ggatgccata
     3841 aaaaatcagt tagacatcat tggggatcaa gagcatgaag atttatttat gggccttttg
     3901 aaacaatact attttgatca ggagatgcat ttggatacaa aagctgctct acctacgatt
     3961 ggaatgtcag attcattgcc taaattgatt atccatggaa cagcagatga tgtagtacct
     4021 gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt ataaagatct tttattagta
     4081 gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg ctgcctacac gaaacatgta
     4141 acggactttt tgaaagtagt gttccatgat cagataaacg taaagtatgt ggatgaaaac
     4201 aataaaagtc tactcaaaga ccaggacgaa atccagcttt acggagctta tggagaaaat
     4261 tatatgacag agcaaaagac attcgaagga tacgaattag caaatgttga aggaccgact
     4321 gaagggattt ttaatgagac gataccaact attattttct attacaaaaa aatcccagta
     4381 gctccaccaa aaaagcaaga tccgacagag aatgcagaca ataagggaaa gaatgaacct
     4441 cgagaggatc aatcaagtga tgatacgcaa ccgattcaga tgacagtgaa agtacaaaaa
     4501 aagacagatg caaaaaaaag aatgttgccg aaaactggag aaaagaaaca ttcgcttctc
     4561 atttattcag gtggattttt atctgttagc acgattatca catggatttg gcgaagaaag
     4621 agagggtaaa tgaatgaaat ggttcaagcg aatagtgatt gcagtaccag ttatcatcgt
     4681 agttatttta gtcactgtat ttttgattaa tcaaatcaca ccaaagccga ttagcttgct
     4741 tgttagaaaa caatttgaca caagtgaaaa agaacaagta tatgcccgac cagatgattt
     4801 ccagcaaaaa atcgaacaaa tcgatataaa aaaagaaata tcctatcctt caaagtacaa
     4861 aaataataat atggatatct acacaccgaa agtggaaaag aaaaaattac cgattctttt
     4921 ctggatgcat ggaggcgcat atgtaggagg ggataaaaat gattgtcgtg attatttgga
     4981 atatctatgt tcagacacgc agcaaatcat cgtaaatata gattatgaac gatcaccgga
     5041 agcaaaacat cctacaccag ttatccaatt gaacgaagcc attcaagcag ctaaaaaaga
     5101 gataaaagat caagcagatt ggtcgaatat ttcgattggt ggaaattcgg caggtgcaca
     5161 aattagtgga gaatatttgt tagcacttca aaatgaagaa attaaaaaag ctgatcatct
     5221 tgatccgact ttggagaaca agcagattac gaagtttatt tctttgtctg gtctgttgga
     5281 tccttctgca cttactcaag taagtgataa aatctctagc tttctttatg aaaaatgtgg
     5341 atgggcttac tttgataata aggactttga acaatcagag aaggtcaaaa gtttagcctt
     5401 ggtcagacat gcggatcgtt ggacgcaaaa cacattctta acggatggaa atacaaatac
     5461 gtttacgaaa caaatggaag aagtaacttc tgtatttgag aaagcggggg tgaaagtcac
     5521 aaaagtagat tatgcggaga aaaatgctgt tttgaaccat gaatatcagt ttgatttcag
     5581 caataaacaa gcgcaagaaa catatcaaaa gttagtaatg tttttcaagg agtaaagtct
     5641 tatgatgcaa aagaggttgt aatctgtcac ttagctccaa gtcacaagga gcaatttcaa
     5701 taaacaagct tttcatggtt tatgaatatt gtaacttgtg actgaggagt tagagatggc
     5761 aaggggcacc ttttattcga aatttgggaa tatcacacga ttattgtcac gatctctttt
     5821 acgtaaattt tataagtcag atttgttgtt ttgatcgttt aaaaaaagca acaaatccga
     5881 agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa gcaaaagaat gctggttcca
     5941 ctaatattcc gttgttattg atataaggct gggaacaaag atgaatagca aataaaatca
     6001 atgcactagc gaaaaaaagc aaagaaatat ggatagcttt cataagcagc acctcctttt
     6061 ttataattgt atgataaata gattaagaat ctataaatgt gacaagtttg ttaggatgtg
     6121 aataaaatga tcatctagcg aacttgaaac aaagatggct aatgataagc aattaatgat
     6181 tcagcaatca gataggtaca aagtttacca tcatacgtct agaccaataa tagaaaatat
     6241 gggaatataa tagaaaagat ttaggatacc cccaaaagaa tttccccaaa aattcgatgt
     6301 atcctaaact tttttatttt acataacaat catatcaact gtgaaataaa attacaaatg
     6361 ataacctaaa atgag
//
LOCUS       DADJYG010000132         6303 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.137, whole genome shotgun sequence.
ACCESSION   DADJYG010000132 DADJYG010000000
VERSION     DADJYG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.137"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            334..714
                     /locus_tag="JZ382_002200"
     CDS             334..714
                     /locus_tag="JZ382_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY2428353.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="JZ382_002201"
     CDS             696..1610
                     /locus_tag="JZ382_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY2428354.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1570..1848
                     /locus_tag="JZ382_002202"
     CDS             1570..1848
                     /locus_tag="JZ382_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428355.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            1855..2544
                     /locus_tag="JZ382_002203"
     CDS             1855..2544
                     /locus_tag="JZ382_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428356.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="JZ382_002204"
     CDS             complement(2665..2751)
                     /locus_tag="JZ382_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAY2428357.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="JZ382_002205"
     CDS             3427..4377
                     /locus_tag="JZ382_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAY2428358.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="JZ382_002206"
     CDS             4398..4931
                     /locus_tag="JZ382_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAY2428359.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="JZ382_002207"
     CDS             5567..6100
                     /locus_tag="JZ382_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428360.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DADJYG010000133         6303 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.138, whole genome shotgun sequence.
ACCESSION   DADJYG010000133 DADJYG010000000
VERSION     DADJYG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.138"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(90..278)
                     /locus_tag="JZ382_002208"
     CDS             complement(90..278)
                     /locus_tag="JZ382_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY2428361.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            548..910
                     /locus_tag="JZ382_002209"
     CDS             548..910
                     /locus_tag="JZ382_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAY2428362.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            962..2644
                     /locus_tag="JZ382_002210"
     CDS             962..2644
                     /locus_tag="JZ382_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAY2428363.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2761..4797
                     /gene="recG"
                     /locus_tag="JZ382_002211"
     CDS             2761..4797
                     /gene="recG"
                     /locus_tag="JZ382_002211"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY2428364.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4846..5847
                     /gene="plsX"
                     /locus_tag="JZ382_002212"
     CDS             4846..5847
                     /gene="plsX"
                     /locus_tag="JZ382_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY2428365.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5969..6211
                     /gene="acpP"
                     /locus_tag="JZ382_002213"
     CDS             5969..6211
                     /gene="acpP"
                     /locus_tag="JZ382_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY2428366.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2099 a         1096 c         1433 g         1675 t
ORIGIN      
        1 aaaaagggta ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc
       61 actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa
      121 gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt
      181 aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt
      241 tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg
      301 atttggagca atagaataat tcagctcata ccagactaac atatcatata gaatgggaat
      361 ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt aaattaaaca
      421 aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca
      481 agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg
      541 ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt
      601 aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag
      661 caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg
      721 agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta
      781 tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga
      841 ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact
      901 gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta
      961 ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa
     1021 tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg
     1081 cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc
     1141 aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc
     1201 gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc
     1261 agatgttgtc tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac
     1321 tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc
     1381 agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc
     1441 tgttgtaaaa ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa
     1501 agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt
     1561 aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat
     1621 ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga
     1681 tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga
     1741 tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag attctttact
     1801 tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt
     1861 cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt
     1921 gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat
     1981 accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt
     2041 tgaaagttta ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga
     2101 agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa
     2161 taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt
     2221 tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc
     2281 ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca
     2341 agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt
     2401 tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt
     2461 aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga
     2521 tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga
     2581 tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga
     2641 ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt
     2701 agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt
     2761 atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct
     2821 agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc
     2881 tacgaagata ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg
     2941 aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg
     3001 caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat
     3061 ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag
     3121 cgtaaagcct tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg
     3181 atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg
     3241 tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat
     3301 cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa
     3361 aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt
     3421 caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc
     3481 caaaaattga aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt
     3541 gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag
     3601 ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca
     3661 gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa
     3721 agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg
     3781 aaagcaaaag aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg
     3841 atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt
     3901 acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg
     3961 cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc tattacagca
     4021 tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa
     4081 acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg
     4141 gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat
     4201 gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa
     4261 gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc
     4321 aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg
     4381 ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac
     4441 caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat
     4501 cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt
     4561 gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa
     4621 tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta
     4681 gccagagaag aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat
     4741 cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag
     4801 actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta
     4861 gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag
     4921 caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac
     4981 ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat
     5041 gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca
     5101 gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca
     5161 gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc
     5221 ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca
     5281 gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa
     5341 gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa
     5401 ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt
     5461 attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat
     5521 ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat
     5581 ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta
     5641 aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt
     5701 ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt
     5761 cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc
     5821 gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga
     5881 aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac
     5941 aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc
     6001 gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat
     6061 caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga
     6121 tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc
     6181 agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa
     6241 gctgcatgca gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt
     6301 ctg
//
LOCUS       DADJYG010000134         6173 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.139, whole genome shotgun sequence.
ACCESSION   DADJYG010000134 DADJYG010000000
VERSION     DADJYG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.139"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            725..811
                     /locus_tag="JZ382_002214"
     CDS             725..811
                     /locus_tag="JZ382_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAY2428367.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="JZ382_002215"
     CDS             complement(932..1621)
                     /locus_tag="JZ382_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428368.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="JZ382_002216"
     CDS             complement(1628..1906)
                     /locus_tag="JZ382_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428369.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="JZ382_002217"
     CDS             complement(1866..2780)
                     /locus_tag="JZ382_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY2428370.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="JZ382_002218"
     CDS             complement(2762..3142)
                     /locus_tag="JZ382_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY2428371.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="JZ382_002219"
     CDS             complement(3592..3879)
                     /locus_tag="JZ382_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAY2428372.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="JZ382_002220"
     CDS             complement(3899..4123)
                     /locus_tag="JZ382_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAY2428373.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4781)
                     /locus_tag="JZ382_002221"
     CDS             complement(4578..4781)
                     /locus_tag="JZ382_002221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428374.1"
                     /translation="MAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKGNKKG
                     ELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="JZ382_002222"
     CDS             complement(4763..5296)
                     /locus_tag="JZ382_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAY2428375.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="JZ382_002223"
     CDS             complement(5317..6159)
                     /locus_tag="JZ382_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAY2428376.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DADJYG010000135         5984 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.141, whole genome shotgun sequence.
ACCESSION   DADJYG010000135 DADJYG010000000
VERSION     DADJYG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.141"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(170..3319)
                     /locus_tag="JZ382_002224"
     CDS             complement(170..3319)
                     /locus_tag="JZ382_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAY2428377.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="JZ382_002225"
     CDS             complement(3333..4550)
                     /locus_tag="JZ382_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY2428378.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5984)
                     /locus_tag="JZ382_002226"
     CDS             complement(4540..>5984)
                     /locus_tag="JZ382_002226"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAY2428379.1"
                     /translation="ESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPE
                     YLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVT
                     ITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVT
                     LDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHG
                     VDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLA
                     PKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMP
                     ANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAER
                     KDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEK
                     ELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1634 a         1270 c         1000 g         2080 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcat
//
LOCUS       DADJYG010000136         5904 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.142, whole genome shotgun sequence.
ACCESSION   DADJYG010000136 DADJYG010000000
VERSION     DADJYG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.142"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..986)
                     /locus_tag="JZ382_002227"
     CDS             complement(<1..986)
                     /locus_tag="JZ382_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAY2428380.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="JZ382_002228"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="JZ382_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAY2428381.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="JZ382_002229"
     CDS             1811..2965
                     /locus_tag="JZ382_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAY2428382.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="JZ382_002230"
     CDS             3180..4148
                     /locus_tag="JZ382_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAY2428383.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="JZ382_002231"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="JZ382_002231"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAY2428384.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="JZ382_002232"
     CDS             5178..5786
                     /locus_tag="JZ382_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAY2428385.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa ggca
//
LOCUS       DADJYG010000137         5887 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.143, whole genome shotgun sequence.
ACCESSION   DADJYG010000137 DADJYG010000000
VERSION     DADJYG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.143"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(98..583)
                     /locus_tag="JZ382_002233"
     CDS             complement(98..583)
                     /locus_tag="JZ382_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428386.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(722..937)
                     /locus_tag="JZ382_002234"
     CDS             complement(722..937)
                     /locus_tag="JZ382_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAY2428387.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1161..1961
                     /locus_tag="JZ382_002235"
     CDS             1161..1961
                     /locus_tag="JZ382_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2428388.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1980..3848
                     /locus_tag="JZ382_002236"
     CDS             1980..3848
                     /locus_tag="JZ382_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAY2428389.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3841..4332
                     /locus_tag="JZ382_002237"
     CDS             3841..4332
                     /locus_tag="JZ382_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY2428390.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4320..4874
                     /gene="srlA"
                     /locus_tag="JZ382_002238"
     CDS             4320..4874
                     /gene="srlA"
                     /locus_tag="JZ382_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY2428391.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4892..5887
                     /locus_tag="JZ382_002239"
     CDS             4892..5887
                     /locus_tag="JZ382_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY2428392.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2057 a          943 c         1186 g         1701 t
ORIGIN      
        1 taatgcaaat gcttatttat actccgtata actttctcag aaaaaatgat accaataatg
       61 acttgctagt actgtaaatc cgtataatat catctaatca aaatggtgtt ccatttgcat
      121 ctgtcatcgt aatcgatcct tcgggtgaca ctcgataaaa accaacgctt ccagatcctc
      181 cttgggaacg gatttgtttg ctggaagctg tgaacaaata atctcgatca attttttgat
      241 aaaagctaat aacaatatcg tcactttgta attcggaacg gtgggcaata agaaatagaa
      301 gtgctttgtc agagttttct aatggaagag aaggtacagt gctttcttgc atttcagatg
      361 ttgaagaaat gctaaacgac tgaatacttg attggctatg ttgcgaatct acattaccag
      421 tagaagtatt agtaatggaa gtagtagaag tacttttagt tgaaccaacc tcagttgttg
      481 aaatggctgc tgaagattct ttaacagaat tctttgtttt atatccacag gcagaaaaaa
      541 gtattacagt tcccaacaag cctgcgtaaa tccctttatg catgatagct cctcctaaaa
      601 tagattatct ttattcagta tatcacttaa acaaagaaaa gcagatggtt taaaatgaaa
      661 aaaccttccc aataaaggga aggttcatga agataataac ttttttaata attatagctt
      721 tctaatatct ttcatcaact tttctagtaa tcattgggaa aaatttcaag cagtagatcg
      781 cacaaatacc tacgacgata taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa
      841 tcatagctac taaatcaagt tcgaacaagc ctaccaacag ccagttcaac cctcccacaa
      901 tcaataaagt taatgcaata gcatctaaag ttttcattag aattctcctt tcttaaatga
      961 taaaacaatt atatatcaaa aagagaaaca ttgcttatat aacgctcagg aattttagca
     1021 atctatgcaa aaagatgaaa attttttttg ctcttccatt agtacacatt ttttagctga
     1081 agctgtaaat ttcatgtctt aatttttttt agtgagcttt tatactggat ttgtaagaaa
     1141 aaagaaggag aggatcacat atgtcggatt ggttggatat tgcaggaaaa gtagtaattg
     1201 taactggagg ttcctcagga attggaagaa gtatcgtcga aaatttgttg aagcagaatg
     1261 cacaagtagc gaattttgat gtaactgaat gccgtataca gcatgaaaac ctactatctt
     1321 taaaagtcga tgtgtcttct aaaacggaca taaaagaagg gatatataaa gtaatgaaac
     1381 attttaagac gatcgatggt ctagtcaata atgcaggaat aaatattcct tctcttctaa
     1441 tagatagaaa tcatccaaaa agcaagtatg aattatcaga acaagttttt gataagatga
     1501 tcgctgtcaa tcaaaaaagt gtatatttga tgagccaagc agttggaaga atcttggtgc
     1561 aaaagggatc aggagtgatc gtgaatcttt cttctgaatc aggattggaa ggatcagaag
     1621 gacagagctg ttatgctgct acaaaagcag cgatgaacag ttttactcgt tcttgggcaa
     1681 aagagttagg aaagaggaat gttcgtgtgg taggtgttgc accagggata ctggaagaaa
     1741 ccggtttgag aacacaagaa tatgaagaag ctctttctta tacgagaggt ataagcgtcg
     1801 aacagctgag aaacggttac agtcgaacaa gtacgatccc tctaggaagg agtggtaagc
     1861 tgcaagaagt ggcggatcta gtctgctatt atctttcaga aagatcaagt tatatcactg
     1921 gagtcacgac taatatttca ggaggtaaaa caagaggata aaggataggg gggacgcacg
     1981 tgacgctagt aaacagatgg taccaattat tgaaattact agtcactcac aagaaagttt
     2041 caatccagga agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa aaagatattg
     2101 gggagttgaa tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac aaacattttg
     2161 aacttgaaat ttatgatttt gatttttttg atgagatcat gcaaggaaaa ttgaaaacag
     2221 ccagtgattt caattcggca tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa
     2281 cagaggattt cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc agaggaacag
     2341 tggtcaagga tctaaagaca ttaaaagaga tgatcaatga ttttgatgta aagattacag
     2401 gcacaccaaa taaaggacta agaataatag gagatgagct agaattacga ctacttttat
     2461 tttattttgt ttctccctac ttttcagaga cgaaaacaga acagtttcta gaagaaaaac
     2521 aagcaatcaa acattttcag aagaagacga atgctagtaa acaagaaatc acccttttaa
     2581 cgaaagttat cggtatttct ttgaatagaa tctctagtaa ctatctgttg aagaagccca
     2641 tcaatcgtta cagtgggtgt tttgaatata tccaagaatt caatgaactg atcaatcagt
     2701 tggaagaaat ctatgaacta actttgagcc aatatgaaaa agattttatc agttttcctt
     2761 tgaacatcat caacacaagt ataacgatga taaggaaagc taactctgat ttgcgcttat
     2821 atttcgaccc catgatgatg cgtattcgac gattagatct cattgatctg gatgagtcct
     2881 tcttatatca agagatgaaa cagcacctct ctttattgat aaatcgggta atttttcgtt
     2941 atcgattgac tgatttattt tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa
     3001 tcgctagcga atgcatacaa gcattagaag ctatcttgca acataagatc tctaaagtag
     3061 agatcagcta cctaacgttg tatttggagt tagcatttcg atatcaaaaa gagcattttt
     3121 cctctaaaaa agtagccatt gttcatacga caggaaaagg aacagcatta atgatccaaa
     3181 gacaaatcaa aaggatattg gggagggata tacaaataac acatgtttca gaagaacaat
     3241 atgaaaaagt agatctaaac aaatatttcg ctgtttttac aacgattccg ttaaaaaatg
     3301 taaatgaaga gacaccggtg atccatttga ctaatctttt tgatgactct tggttaaggg
     3361 cacaatggca aaaagcaaaa aaaaagcgat tagccgggtt tcattctact gtttttttct
     3421 atcaaatatg gaaagaaaaa aagacgtatc aagaatattt aaacaaatta gtcgctcctt
     3481 tgatcagagg ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc
     3541 agcaaagcac atttgttaac aaagaatttg cttttcctca cacaaccaac cagctatctc
     3601 aacacattgt tttatcagta gggctaataa ctgatcaagg tttagcagaa acaggaggaa
     3661 atggagttat ttttttattt gccatacctg aagaaatgac ggctgaagtt gagaatgaat
     3721 tatttgagct atacgatatc ctatttgaac tcataaaaat gggaatagat gagtttattg
     3781 aattagcacc gacacaagaa tcatttttaa aactaatcaa aagtaaagga gagagacttc
     3841 atgaatgata tgtatctatt catagttttc gcagcttcag cttatgtgct gcaagcagtt
     3901 atcagttgga agcaactaaa gcatttcaat caaacatata gcgaactgcg aagaaaagga
     3961 aaagtagcga ttggtaaacg acctgggaaa atccgttcag gcacgattgt tatgctagct
     4021 atagacgatc atgatcaagt tttagaagtc agaaaaatgc aaggggttac atctttggca
     4081 aaatttcatt cattacctgt ctatgcgggt gaagatatcc attacatcga tcgttaccat
     4141 ccctcagttc gagcggaaga tcgtttgaca caagcagctt tagaaaatgc cagagaagta
     4201 tacttacgaa tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc ttttattgct
     4261 atgctcaata attttcggat atggaaaagt acgatcaaag aaaaaaggag tgacaaacga
     4321 tggatatcat aacaaaattt gctgagggat ttatgaggct gttccaaaca ggtgcagaaa
     4381 cattcataag ctggatgaca ggaatcgtac cagttgtctt gatgctgatg gttgcaatga
     4441 atacgctgat tgctttttta ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga
     4501 aaaatccgct tactcgttac cttgtgttac cgttcttatc tgcttttatg ttgggaaatc
     4561 ctatgtcatt tacaatggct cgtttccttc cagaatacta caaaccaagc tattatgctg
     4621 ctcaagctca attttgtcat acgagcaatg gtgtattccc ccatattaat cctggagaat
     4681 tgtttgtatg gatggggatt gctcaaggga tcgaaacctt agggctaaat tcgatggagc
     4741 tggctatccg atacttgctt gtgggactgc tgatgaactt tattggtggt tggataactg
     4801 attttactac tggatttgta tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc
     4861 tttcagttga ttagagaaag gagagtttta aatgtatggt gatattaaga tcgtaagagg
     4921 aaacggcggg tatggaggtc ctttgattat taggccgaca aaagataaac acaagttcat
     4981 ctatgtcaca ggtggaggtg agaggccaat gatcgtagat aaaatagaag aactgacagg
     5041 tatggagtct atcaatgggt tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat
     5101 cgttgactgt ggagggactc ttagatgtgg aatttatcct aaaaaaggga taccaaccat
     5161 caatatcgtt cccacaggaa aaagtggccc attagcacaa tatatcactg aagatattta
     5221 tgtttcagca gtagggttag aacaaataca gccactaatg gaaactgatg ataacaaaga
     5281 aacagtaaat actgcatctc cagataaaaa gtacaaatat gatacttcta aaaagataac
     5341 cgagcaaaaa gcagagcagc aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt
     5401 agttgctaca ttcaatcaat cagcaagaga agcagtagat acgatgatcc ggacagtgat
     5461 cccttttatg gctttcgttt cccttttgat tggtgtgata caaggttctg ggatcggtaa
     5521 tgtatttgct aacattatgg cacctttagc aggaaatgca gttggattaa caatcattgg
     5581 ctttatttgt tcactgccat ttttatctcc tttgcttggt ccaggggcag ttattagcca
     5641 agtgattgga acattgatcg gtgtagagat tgggaaagga aatattcctc cccaattagc
     5701 attaccggca ctatttgcta tcaatactca aaatgcatgt gattttattc cagtcggtct
     5761 aggtttggaa gaagcaaaag ctgagacagt agaagtaggt gtaccatcag ttctttattc
     5821 aagatttctt aacggcatcc cacgagttat cattgcttgg gtagcaagtt ttggattata
     5881 tagctga
//
LOCUS       DADJYG010000138         5884 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.144, whole genome shotgun sequence.
ACCESSION   DADJYG010000138 DADJYG010000000
VERSION     DADJYG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.144"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            16..933
                     /locus_tag="JZ382_002240"
     CDS             16..933
                     /locus_tag="JZ382_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2428393.1"
                     /translation="MPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILT
                     FLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTD
                     YDFAHLLFSNGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVIS
                     TFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDD
                     LLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLY
                     GRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            936..3113
                     /locus_tag="JZ382_002241"
     CDS             936..3113
                     /locus_tag="JZ382_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAY2428394.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3110..4111
                     /locus_tag="JZ382_002242"
     CDS             3110..4111
                     /locus_tag="JZ382_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAY2428395.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4108..5040
                     /locus_tag="JZ382_002243"
     CDS             4108..5040
                     /locus_tag="JZ382_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428396.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5285..5401
                     /locus_tag="JZ382_002244"
     CDS             5285..5401
                     /locus_tag="JZ382_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAY2428397.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5417..>5884
                     /locus_tag="JZ382_002245"
     CDS             5417..>5884
                     /locus_tag="JZ382_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY2428398.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ"
BASE COUNT         1884 a         1115 c         1236 g         1649 t
ORIGIN      
        1 agatggaaag aaaccttgcc agagatttcc catgaaatca atatcgtcac tctgacttct
       61 gatgagaaaa acaaaggctt acttgaccct tatgtgatta tgaaaaatcc caaagattct
      121 gaatcactgg ctattgatat tctgacattc cttacgggga tttcctctcg tgatggggaa
      181 cgcttcccaa tccttagaaa agccattcgt gcagtaacca atagtgaagt acgagggttg
      241 atgaaagtga ttgaggaatt acgggttgag aatacgccac taagtaccag tatagccgac
      301 catatcgaaa gttttacaga ctatgacttt gcacatttat tattcagtaa tggttatgtg
      361 gagcagtcta tcagcttaga aaaacaactg aacattatac aggttgcgga cttggtactt
      421 cccgacaagg aaacttcctt tgaggaatat accactatgg agcttttatc cgttgctatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccata cagaccgaag cattttcaag
      541 attgtagatt tagacgaagc atggagcttt ttacaggtag cacaaggaaa aacactatct
      601 atgaagctgg ttcgggctgg tcgtgctatg aacgctgggg tatatttcgt gacccaaaat
      661 acagacgacc tcttagatga aaaactgaaa aataacctcg gcttaaaatt tgcatttcgt
      721 tccactgacc ttaacgagat taaaaagacc ttagcctttt ttggtgtaga cccagaggac
      781 gaaaacaatc agaagcgatt gcgtgatttg gaaaacgggc aatgccttat cagtgattta
      841 tatggtcgtg tcggtgtgat acagttccac cctgtatttg aagaactgct ccatgccttt
      901 gataccagac cacctgtgcg aaaagaggtg taaatgtgaa accatcaata gtaaacagaa
      961 taaaatcaaa ctggacgctg aaacgtctag gtaaagtggc aatgacagtg gctttcacac
     1021 ttgtgattgc catttttctt ttagccatgc tgggaacggt ggttcaagct gcgggcttgg
     1081 tagatgatac ggtcaatgtg gcaaatgaat acagccgata cccacttgaa aactatcaac
     1141 tggattttta tgtggataat agctggggct ggcttccgtg gaactggtcg gacgggattg
     1201 gaaaacaggt catgtatgga ctatatgcca ttaccaattt tatttggaca atcagtttgt
     1261 atgtttccaa tgcgacaggt tacttagtac aggaagccta ttccttagac ttcatttccg
     1321 ctacagcaga ttccattggt aagaatatgc agaccttagc tggtgtgagt gcaaacggat
     1381 tttcaacaga gggtttctat gttggattcc tcttactctt gattttggtt cttggggttt
     1441 atgttgccta tacgggactg ataaagagag aaaccacaaa ggcaattcat gccattatga
     1501 attttgtgct ggtgtttatc ctatcggctt cctttattgc ctacgctccc gactacatta
     1561 aaaaaatcaa tgacttttca tcagacatca gtaatgccag tttatcactt ggcacgaaga
     1621 ttgtcatgcc ccattccgat agtcaaggca aggacagcgt ggacttaatc agagatagcc
     1681 tgttttccat acaggttcag caaccgtggc tactgcttca atacaacagt tcagacattg
     1741 aaagtatcgg tattgaccgt gtggaaagcc tgctctccac cagcccagat tccaacaatg
     1801 gcgaagacag agaaaaaatt gttgcggaag aaattgaaga cagaagcaat accaatctaa
     1861 ccattacaaa gaccattaac cgtttaggta cagtcttctt cctatttgtc ttcaatattg
     1921 ggatttccat atttgtattc ctattaacag gaatcatgat tttctcgcag gtacttttta
     1981 tcatctatgc tatgtttctg cctgtgagct ttattttaag catgattcca tcatttgatg
     2041 gtatgtcaaa acgagccata acaaagctct ttaataccat tttgacacga gctggaatca
     2101 cattgattat tacgacagca tttagtattt caaccatgct ctatacctta tcggctggtt
     2161 atccgttctt tttgattgct tttctacaga ttgtgacctt tgcaggaatc tacttcaagc
     2221 tgggcgattt aatgagtatg ttttctctac agagtaacga ttctcaaagt gtgggaagtc
     2281 gtgtgatgag aaaacctcgt atgcttatgc acgctcacat gcaccgtcta cagcggaaac
     2341 ttggacgttc catgactact ctaggggctg ggtctgccat tgttacaggt aaaaaaggac
     2401 agtcgggttc ggggagttct gcaaggacac aagcagatca ctcccgacca gacggaaagg
     2461 aaaaatcaac acttggaaaa cgtatcggtc aaaccatcgg tacagtagct gataccaaag
     2521 acagaatggt agacactgct agtggtttga aagaacaggt taaagatttg ccgaccaatg
     2581 caagatatgc agtatatcaa ggaaaatcca aagtaaaaga gaatgtccgt gatttaacca
     2641 gtagtatttc tcaaaccaaa gcggacagag ccagtggacg caaggaacag caggaacaaa
     2701 ggcgaaaaac cattgcgaag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc
     2761 ctgcttcttc tgttcatgaa agaccgacta caagacaaga acaatatcat gatgaacaga
     2821 cctcaaaaca gtctaatatt cagacttcat ataaggaatc tcaacaagcc aaacaagagc
     2881 gtccagcagt taagtccgat ttttcaagtc caaaagtgga acgccaaggc aataccgttc
     2941 aagaaaaaac cgttcaaaag ccagcaactt caaccactac agcagataga acttcacaac
     3001 gtccaatcac aaaagaacgt ccgtctactg ttcaaagagt accactacaa aatacaagaa
     3061 gtagaccacc aatcaaaacc gccaccatta agaaagtcgg taagaaacca tgaagttgaa
     3121 aactttagtg attggtggtt ctggattatt cttgatggtc ttctcactgc ttctgtttgt
     3181 tgccatttta ttttcagatg aacaggacag cggaatttcc aatattcatt atggaggtgt
     3241 gaatgtttcc gcagaagtgc tggctcataa gcctatggta gaaaaatatg ccaaagaata
     3301 tggcgttgaa gaatatgtca acatacttct tgcgattata caggtggaat cgggcggtac
     3361 tgcggaagat gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac
     3421 agaagaatcc attaagcaag gtgtgaagta tttcagtgaa ttattagcca gtagcgaaag
     3481 gctcagtgta gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg
     3541 gtatgtggct aatcgtggaa ataaatatac ctttgaactg gctcaaagtt tctcaaaaga
     3601 gtattcaggt ggcgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg
     3661 ctggcgatac aactatggca atatgtttta tgtgcaactg gtaacgcagt atcttgtcac
     3721 aacagagttt gatgatgata cggtacaagc catcatggac gaagcactga aatatgaggg
     3781 ctggcgatac gtttacggtg gagcttcccc gactacttct tttgattgta gcggactgac
     3841 acaatggacg tatggaaaag ctggaattaa cttaccacga accgcacaac agcaatatga
     3901 tgtgacccag catatcccac tatcggaagc acaagctggc gatttggttt tctttcattc
     3961 tacctataac gctggctctt atattactca tgttgggata taccttggca ataaccgtat
     4021 gtttcatgca ggcgacccaa tcggttatgc cgacttaaca agcccctact ggcaacagca
     4081 tttagtggga gcaggacgaa tcaaacaatg agaaaggaag atttaatgat gaaatttaga
     4141 aaaaatcaga ataaagaaaa acagatacca aaggaaaaga aacctcgtgt ctataaggtc
     4201 aatcctcata aaaaggttgt gattgccttg tgggtacttt tagggcttag tttcagcttt
     4261 gcgatattca agcactttac agctatagat actcatacta ttcacgaaac aactatcata
     4321 gaaaaggaat acgttgatac tcatcatgta gaaaattttg tagagaactt tgcgaaagtc
     4381 tactattcat gggagcaatc cgataagtcc attgataatc gaatggaaag tctaaaaggc
     4441 tatctgacag atgaacttca agctctcaat gttgatacag tacgcaaaga tattcctgta
     4501 tcgtcttctg taagaggatt tcagatatgg acggtagagc caactggcga caatgagttt
     4561 aatgtaacct acagtgtaga ccagctcatt acagagggag aaaatacaaa gaccgtccac
     4621 tctgcttata tagtgagtgt ctatgtagat ggttctggaa atatggtact ggttaagaat
     4681 ccgaccatta ccaacatacc taagaaatca agttataaac caaaagccat tgaaagtgag
     4741 gggacggttg attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag
     4801 ctctatccta cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa
     4861 ccaatcggaa aagagtacat ctttcaagaa ctggtaaatc ctattcacaa tcgtaaggat
     4921 aatcaagtca cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag
     4981 gtatctcaat ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa
     5041 caaatattgg tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt
     5101 ggagattcct ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag
     5161 ttaataaata tgcggcaagg tattattaaa taaactgtca atttgatagc gggaacaaat
     5221 aattggatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
     5281 tcatgtgatt ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca
     5341 taaaaatccc agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg
     5401 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
     5461 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
     5521 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
     5581 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
     5641 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
     5701 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
     5761 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
     5821 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
     5881 acag
//
LOCUS       DADJYG010000139         5756 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.145, whole genome shotgun sequence.
ACCESSION   DADJYG010000139 DADJYG010000000
VERSION     DADJYG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.145"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            83..979
                     /locus_tag="JZ382_002246"
     CDS             83..979
                     /locus_tag="JZ382_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAY2428399.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1129..2070)
                     /locus_tag="JZ382_002247"
     CDS             complement(1129..2070)
                     /locus_tag="JZ382_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY2428400.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2196..3317
                     /locus_tag="JZ382_002248"
     CDS             2196..3317
                     /locus_tag="JZ382_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAY2428401.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3575..5077
                     /locus_tag="JZ382_002249"
     CDS             3575..5077
                     /locus_tag="JZ382_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAY2428402.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5144..5671
                     /locus_tag="JZ382_002250"
     CDS             5144..5671
                     /locus_tag="JZ382_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY2428403.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1878 a         1021 c         1218 g         1639 t
ORIGIN      
        1 atataggaaa cgttttactg gctttttact atttaaacag gctatcatag gaaaaagtga
       61 cagagaaagt gaggcggaaa agatgaacaa aaatgacgta ctgctagttg caaaaaataa
      121 atcactttat tttttgcctt ccatccgctc tttagatgtt tggtccggaa aagatttact
      181 acagtcggaa ttcattaaat cagctagaca acagttggca aattatcagc tgatcgtttt
      241 tttagattat ggatttgata tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa
      301 aattgtgtta tttttttgga atcacttcaa agcagaacac gaggcgatgc tgaagcaggc
      361 acaagatgaa ccggcaatcg atgagattta tcattttgat tttttggaag caaaagaact
      421 tgggctgaaa cataatagtt cattttattc gaaacatatg aatttttctt ctagcgaacc
      481 aaagacggat ctattttttg gtgcaacgaa taatgggaga aaagaacgag cagaaagcta
      541 taagcaggaa tttataaaaa gagatgttac gaccaactat ttcattcttc cacatcgagg
      601 gaatgaacaa cctggatatc ttacttatga agagtacctg aaaaaaacag gggaatcgcg
      661 cgggattctg gaactgttgc gggagggtca gcaaggagtg actttgcgga catttgaatc
      721 catgtatttt caaaagaaac tagtgacgga taatcaagcg attgctcatt atcattttta
      781 tcgtcctgaa aatatttttt tgcttcgaga acgtgattta gatgaattac cggaatttct
      841 gcatacacca tttaagccaa tcctatcaga gctattggaa ttttatgatg cagaaaattg
      901 ggcaaaacga tttatcacta acgattccac tatttatgag caatacgaat atgacctctc
      961 ctctgttgaa aacaaataaa aaacctacat tttgaagaga ttatgtactg aaacaaacaa
     1021 gcacaattct tatggcatca tgagagttgt gttttctttt tgatctttta ttgggcttaa
     1081 aaaaagagaa ccagctgctg attcagctga ttctcatcag agagcctttt attctgcgcc
     1141 aaggattcct tcttttgcat ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat
     1201 atcatatttt tgtccttttc gccaaatggc accattgatg tagtcgattt cagtcaaacg
     1261 atggtttttg atcaggtctt ggtacataga tgggtaatgc aacccgatgt tgtcaggatc
     1321 ataagtagaa gcaatatgtt ggtacacttc ttcttgatct agtatgattc cttcttttgc
     1381 tgcgatagaa gcaaattcag atacgattgt tcgaaccatg ctctctgccg ctttttgcgc
     1441 gcctagttca gcaatattac aatccaaaat cgtgcataaa ccattcaatg ttccatttac
     1501 acaagctttt cgccaaatgg aataacgaac gttatggcta tagatcggat ttaaatttgc
     1561 ttcttgaaag acttctacaa ctttttttgt aaatgcttcg ccttctggtt ccaagttctc
     1621 tagttcgatt tctccatcac ctaacaatgt gacttttccg ggaccagcta gaccagctgt
     1681 ccacatcgtg atcccaaata agatattttt ctttggcaca tatttttcca gtacatcttc
     1741 atgacctaat ccatttagta aacacaaaac ataagtattg tcagtaataa tggattggat
     1801 cgaacaaaac atatcatcta attgattggc ttttgttaat gctacgatca agtccacgtg
     1861 ttcgttgctt tcgatgattt cttctggtga atagattggc agttttgcga ccacttcttt
     1921 accgttaaag tcagcaatca acccatttgt acgaatggct tcaatgtgag caggccaacg
     1981 atcgatcaag aggacctcat ttccgctttg atgtagcatc aatccgattc ggctgcccat
     2041 tgctccggcc ccagctattg cgattttcat gttttttctc tcctttattc aatgagttcc
     2101 cttttagtat atagaaaaat ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa
     2161 aagtgtctta cttaagaaga aagtgaggga tacagatgga tattttggcg gcaattgatt
     2221 catttaaagg aagtgcttct tcatttgaac taaatcaggc agttctaaat ggctgggaag
     2281 atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg cggagaaaat actgcagaag
     2341 caatctatta cgcgctcagg ggaacatggc gtaccattga tgggatggat ctcttattcc
     2401 gtcagcgaaa aatcagctat ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg
     2461 aatctgcaca agcattgggg ttagatctac ttgataaacc aacggatcaa actatccgac
     2521 ttgcttccag ttatgcctta ggagaactta ttaaagatgc actgcttcaa gaagtagaac
     2581 aaattattgt cactttagga ggaagcgggt gtagcgatgg tggtctagga ttactccaat
     2641 ccttgggtgc ttctttagaa gggtacacag aaggaaatcc tttattatct actaaaaaac
     2701 ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa attgattatc gctgcagatg
     2761 ttaccaatcc atatacaggt caacaaggag ctgcttggca atttggaaga caaaaaggag
     2821 gaatagatac gacgctagct ttcttagata aacaagcagg aaagattgct cagcaaataa
     2881 aagatttctg ggaggtggat ttagatgata ttccaggaag tggagcagca ggcggtattg
     2941 gtggagccct ttttttgttg ggagcgaaga tggtttctgg tttcgattta gtcagccaaa
     3001 tcactggatt ggaaaaaaag atcgaacaag cagatgtcat cattacagga gaagggcgga
     3061 tcgatcagca aacgatccac ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat
     3121 atcgaaaacc aataatcgct atttgcggaa gtagagacca agagctagaa ggactttctg
     3181 attttctccc aattgtctta agtattcatt tgggtccaat aggtttagaa gaagcaatgg
     3241 aacacaggca aacattagaa aaaacaaaaa tcttaggaca gacacttagc agatttttca
     3301 ccatcctccc gaagtagttg acaaaatcat tttctttatg ctatcgtaat ttcaaatata
     3361 ggacaaagtg agggatccgt gcattgaaaa attaagtcga ttgatgaaag ctgcattcag
     3421 actgtacctt gtggagacaa ggaagcagtg tgcagatttt cgggattggc ttggtttttt
     3481 cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc ctctttagcc agttctgaaa
     3541 ataggactgg tcttttttta cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct
     3601 gacattcggc tacgacagcc aaggcacatt attatttgaa caagcaaatc taaattttga
     3661 cacacaatgg aaactaggac ttatcggacg aaacggtcga ggaaagacaa ctttactgaa
     3721 tattctacaa aacaaactac cttatcaagg gcaagtaatc catcagcaag aatttgccta
     3781 tttcccgcaa cagacaaaag ataaagaacg tttaacctat tacgtgttaa atgatattac
     3841 ggattttgag atatgggaaa tcgaaagaga gctccaattg atgcaaacag atcctgaaat
     3901 cttatggaga gaattcagca cactatcggg gggagagaag acaaaagtcc tactggcact
     3961 tttatttgtg gatgacactc atttcccgtt aatcgatgaa ccaacgaatc atttggatat
     4021 ctctggtaga aaacaagtag cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt
     4081 cagccatgac cggggattta tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag
     4141 tcaactggaa ctttatcaag ggaatttctc tatctatgaa gaacagaaaa aacttcgtga
     4201 tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac
     4261 agcagctgaa aaagccgaat ggtctcgttc ccgagaagga gataaaacaa agaaacaagt
     4321 cggattcatc gatactgaat ctagacgagt gaataaagga gcagtgggtg ctgatgctgc
     4381 acggacgatg aaacgatcca aagcaatcgt gaatcggatg gagacccaga tcagcgagaa
     4441 agaaaaacta ttaaaagata tcgaatatat cgattctttg acgatgaata gccaagcgtc
     4501 tcaccataag cgacttttaa gcgtagaaga tcttcaatta gggtatgaaa atctgttatt
     4561 cgagccaatt cattttacaa tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg
     4621 tgcaggaaag tcatccatta tccattatct tctgggggca ttcaacggca aggttatagg
     4681 agaaaaatac cagccaaaac atctgagcat tagttatgca agccaaaatt atgaagacaa
     4741 tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta gactaccaag catttttgaa
     4801 caacctccga aagcttggga tggaaagaga tgtttttcat aacaagatcg agcagatgag
     4861 tatgggccaa cggaaaaaag tggaattggc taaatcttta tcacagccag ctgaactata
     4921 tacatgggat gaaccattga attatttgga tgtcttcaat caagaacaat tagaacaact
     4981 gatcttgaac gtgaaacctg ccatgttact agtggaacat gatcaaacct tcctggataa
     5041 agtatctact gagattattt ctcttgagag aatctaaccc ttgtaatctc aagaacaaca
     5101 aggtatggtt aaggggaact ttgccaacga aggagagaat cacatgcttt atgtaacgag
     5161 acacggagaa acaacttgga acgcacaagg attagtttgc ggacgggcag atgtgccgct
     5221 gactgaaaaa ggacagatgc aggcacagaa actagcagaa aaggtagtag atttgcctgt
     5281 tccaatcacg aaaatcatcc attcccctct tcaacgagca agagatacag cacaagcagt
     5341 tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga ttggtagaaa tggattttgg
     5401 ggattatgat ggactgccaa gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc
     5461 tgtacgattc ccaaatgggg agtcagtctt agatgtgtat gcacggatcg ttcctttatt
     5521 gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta gtttgccata atgccttgat
     5581 aagagtgatc aatgcttatt ttcatccgat gcctaatgag gggttcttta cattcatggt
     5641 agataataca gaattggttt cttatgagta attatgtttt tgtgttattt aaataaaata
     5701 tttcaaggca ctgcttttgc ttagcagtgc cttttttagg ttatgacaat tctttt
//
LOCUS       DADJYG010000140         5744 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.146, whole genome shotgun sequence.
ACCESSION   DADJYG010000140 DADJYG010000000
VERSION     DADJYG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.146"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(52..522)
                     /locus_tag="JZ382_002251"
     CDS             complement(52..522)
                     /locus_tag="JZ382_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAY2428404.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="JZ382_002252"
     CDS             complement(681..1139)
                     /locus_tag="JZ382_002252"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY2428405.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="JZ382_002253"
     CDS             complement(1310..2455)
                     /locus_tag="JZ382_002253"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY2428406.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="JZ382_002254"
     CDS             complement(2468..3385)
                     /locus_tag="JZ382_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAY2428407.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="JZ382_002255"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="JZ382_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY2428408.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            complement(4516..5688)
                     /locus_tag="JZ382_002256"
     CDS             complement(4516..5688)
                     /locus_tag="JZ382_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428409.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRIGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LIASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWITFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         1722 a         1059 c         1084 g         1879 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtagtcaa gtccacaatc ctgtgtaaaa tactatacaa
     4501 tgctctcact attttttact tggcaaattt aatatatttt cttgatgaag aaagccactc
     4561 atcgtgttga tcaataagga ctgctccgat taaacgattg gctgaaacgc agtttggaaa
     4621 gatacgaata attttttctc ttctgcgcac ttcttgattc agtcgttcaa ttagattggt
     4681 actctttagt cgattgtggg aatttccttg tacggtatat tgaaaggcgt cttcgaatcc
     4741 atcatccaat gatgcgcaag cttttgaata ttttggttga tcgatataat catgaatcaa
     4801 tcgattttta gcctcacgag ctaagttaat atctgtgaac ttaaaaattc ctttaacagc
     4861 ttctctgaaa gattttgaat tttttttagg aatggtggta aagatatttc ttaggaagtg
     4921 aacttggcat ctttgccaac ttgcgttggt gaaggatttt ctaatggcag agactaatcc
     4981 tttgtgcgca tcagaaataa cgagttctgt accttgtaaa ccgcgttctt ttaggtattc
     5041 aaaaaatgtt atccaggtct cttcgctttc gccactttga atcatgaagc cgataatttc
     5101 acggtcgcca tctttggtta ttccaatcgc tatatgacag ctttttgaga gtactcgatt
     5161 ttcttctcgt acttttatat agagtacatc ggtcattaag taaggataat ttttttctga
     5221 taataaacga ttctgccact cgttaaccat tggttctagc tgttctgtta agctagaaac
     5281 gaaggactta gagacggatt taccacaaag ttcttccaca atttttgata ctttacgagt
     5341 tgaaacgcct gatacataca tttccaacat tgaagcaatg agggcttttt cgtttcgttg
     5401 ataacgttca aacactgtgg gtgaaaaatg gccatcacgt gttctgggta cttttaattc
     5461 tagcgtgcct atacgtgtcg taaagctgcg ctcataatag ccatttcgtt gactttgtcg
     5521 gttttctgtt cgttcatatt cttttgcttg aatatattct gttcgttgat tttccattag
     5581 ttgattaaat accgttgtta aaatattttt agaaacgtca tcctttacag aatattcaat
     5641 aatgctttga atctcttcgc ttttcagtgt aaaatgtact tgggtcatgt aaaagtcctc
     5701 ctgggtatgt ttttgtcgtt aaaaacattg taccgtaaaa ggac
//
LOCUS       DADJYG010000141         5741 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.147, whole genome shotgun sequence.
ACCESSION   DADJYG010000141 DADJYG010000000
VERSION     DADJYG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.147"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(244..951)
                     /locus_tag="JZ382_002257"
     CDS             complement(244..951)
                     /locus_tag="JZ382_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428410.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1016..1852)
                     /locus_tag="JZ382_002258"
     CDS             complement(1016..1852)
                     /locus_tag="JZ382_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2428411.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1870..3279)
                     /locus_tag="JZ382_002259"
     CDS             complement(1870..3279)
                     /locus_tag="JZ382_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2428412.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3293..5116)
                     /locus_tag="JZ382_002260"
     CDS             complement(3293..5116)
                     /locus_tag="JZ382_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428413.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5396..5623)
                     /locus_tag="JZ382_002261"
     CDS             complement(5396..5623)
                     /locus_tag="JZ382_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428414.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1752 a         1251 c          945 g         1793 t
ORIGIN      
        1 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
       61 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
      121 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
      181 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
      241 tttttaccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
      301 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
      361 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
      421 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
      481 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
      541 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
      601 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
      661 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
      721 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
      781 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
      841 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
      901 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
      961 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
     1021 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
     1081 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
     1141 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
     1201 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
     1261 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
     1321 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
     1381 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
     1441 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
     1501 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
     1561 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
     1621 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
     1681 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
     1741 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
     1801 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
     1861 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
     1921 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
     1981 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
     2041 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
     2101 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
     2161 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
     2221 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
     2281 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
     2341 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
     2401 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
     2461 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
     2521 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
     2581 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
     2641 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
     2701 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
     2761 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
     2821 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
     2881 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
     2941 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
     3001 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
     3061 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
     3121 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
     3181 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
     3241 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
     3301 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
     3361 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
     3421 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
     3481 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
     3541 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
     3601 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
     3661 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
     3721 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
     3781 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
     3841 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
     3901 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
     3961 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
     4021 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
     4081 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
     4141 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
     4201 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
     4261 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
     4321 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
     4381 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
     4441 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
     4501 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
     4561 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
     4621 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
     4681 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
     4741 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
     4801 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
     4861 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
     4921 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
     4981 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
     5041 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
     5101 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
     5161 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
     5221 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
     5281 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
     5341 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
     5401 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
     5461 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
     5521 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
     5581 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
     5641 tcaatagaat gatttgggct agtattctta aaaattaact agcaaatgtt taaactgatt
     5701 tatttctaat ctacatgtag taagtatttc aattccacca a
//
LOCUS       DADJYG010000142         5622 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.148, whole genome shotgun sequence.
ACCESSION   DADJYG010000142 DADJYG010000000
VERSION     DADJYG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.148"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..288)
                     /locus_tag="JZ382_002262"
     CDS             complement(<1..288)
                     /locus_tag="JZ382_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428415.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="JZ382_002263"
     CDS             430..1548
                     /locus_tag="JZ382_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY2428416.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="JZ382_002264"
     CDS             1676..3052
                     /locus_tag="JZ382_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY2428417.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="JZ382_002265"
     CDS             3453..5033
                     /locus_tag="JZ382_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY2428418.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="JZ382_002266"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="JZ382_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
BASE COUNT         1881 a          922 c         1148 g         1671 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt tt
//
LOCUS       DADJYG010000143         5616 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.149, whole genome shotgun sequence.
ACCESSION   DADJYG010000143 DADJYG010000000
VERSION     DADJYG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.149"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(270..773)
                     /locus_tag="JZ382_002267"
     CDS             complement(270..773)
                     /locus_tag="JZ382_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAY2428419.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1132..2157
                     /locus_tag="JZ382_002268"
     CDS             1132..2157
                     /locus_tag="JZ382_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAY2428420.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2480..3358
                     /locus_tag="JZ382_002269"
     CDS             2480..3358
                     /locus_tag="JZ382_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428421.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3834..3950
                     /locus_tag="JZ382_002270"
     CDS             3834..3950
                     /locus_tag="JZ382_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAY2428422.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4878..5216
                     /locus_tag="JZ382_002271"
     CDS             4878..5216
                     /locus_tag="JZ382_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428423.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5310..5540
                     /locus_tag="JZ382_002272"
     CDS             5310..5540
                     /locus_tag="JZ382_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428424.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1889 a          845 c         1042 g         1840 t
ORIGIN      
        1 gtagggtata aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt
       61 taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc
      121 ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa
      181 tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg
      241 tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat
      301 aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct
      361 aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct
      421 cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga
      481 atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt
      541 ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg
      601 gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca
      661 ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac
      721 cataatagaa agaataaata aatccctaat aaaaattttg atacataccg catagacatc
      781 tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt
      841 ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt
      901 ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa
      961 aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact
     1021 aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg
     1081 catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag
     1141 acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta
     1201 cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt
     1261 tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg
     1321 aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg
     1381 acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc
     1441 gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc
     1501 atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt
     1561 atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct
     1621 gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc
     1681 attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg
     1741 aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac
     1801 ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt
     1861 gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact
     1921 tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt
     1981 cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg
     2041 atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg
     2101 gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag
     2161 gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag
     2221 gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac
     2281 tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
     2341 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
     2401 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
     2461 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
     2521 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
     2581 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
     2641 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
     2701 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
     2761 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
     2821 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
     2881 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
     2941 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
     3001 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
     3061 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
     3121 actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc
     3181 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
     3241 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
     3301 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
     3361 ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca
     3421 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat
     3481 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
     3541 tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact
     3601 agctataaca atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac
     3661 gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt
     3721 taacaagttg actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt
     3781 cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa
     3841 aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac
     3901 gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa
     3961 acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt
     4021 gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag
     4081 aatctttctt ttcaaattga taccattccc taattccagt tatatagtta actacagaac
     4141 cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac
     4201 ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga
     4261 aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc
     4321 atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa
     4381 gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa
     4441 aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc
     4501 cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat
     4561 aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt
     4621 aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat
     4681 taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt
     4741 gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat
     4801 gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta
     4861 gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc
     4921 gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta
     4981 gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta
     5041 tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg
     5101 aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca
     5161 ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac
     5221 tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat
     5281 cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt
     5341 attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc
     5401 gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa
     5461 ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt
     5521 atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt
     5581 attgtttttt cgatatcttt gctgttaata ttacta
//
LOCUS       DADJYG010000144         5599 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.150, whole genome shotgun sequence.
ACCESSION   DADJYG010000144 DADJYG010000000
VERSION     DADJYG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.150"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(59..2008)
                     /locus_tag="JZ382_002273"
     CDS             complement(59..2008)
                     /locus_tag="JZ382_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAY2428425.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="JZ382_002274"
     CDS             complement(2050..3108)
                     /locus_tag="JZ382_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAY2428426.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="JZ382_002275"
     CDS             complement(3227..3682)
                     /locus_tag="JZ382_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428427.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="JZ382_002276"
     CDS             complement(4266..5069)
                     /locus_tag="JZ382_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428428.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1650 a         1107 c          777 g         2065 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttccc
//
LOCUS       DADJYG010000145         5572 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.151, whole genome shotgun sequence.
ACCESSION   DADJYG010000145 DADJYG010000000
VERSION     DADJYG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.151"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            66..767
                     /locus_tag="JZ382_002277"
     CDS             66..767
                     /locus_tag="JZ382_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAY2428429.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="JZ382_002278"
     CDS             764..1885
                     /locus_tag="JZ382_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAY2428430.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="JZ382_002279"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="JZ382_002279"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY2428431.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="JZ382_002280"
     CDS             3264..4172
                     /locus_tag="JZ382_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAY2428432.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="JZ382_002281"
     CDS             4207..4479
                     /locus_tag="JZ382_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAY2428433.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="JZ382_002282"
     CDS             4490..5536
                     /locus_tag="JZ382_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAY2428434.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         1808 a          969 c         1210 g         1585 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aa
//
LOCUS       DADJYG010000146         5443 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.152, whole genome shotgun sequence.
ACCESSION   DADJYG010000146 DADJYG010000000
VERSION     DADJYG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.152"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            367..555
                     /locus_tag="JZ382_002283"
     CDS             367..555
                     /locus_tag="JZ382_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428435.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="JZ382_002284"
     CDS             545..805
                     /locus_tag="JZ382_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428436.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="JZ382_002285"
     CDS             846..1073
                     /locus_tag="JZ382_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428437.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="JZ382_002286"
     CDS             1138..1710
                     /locus_tag="JZ382_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428438.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="JZ382_002287"
     CDS             1711..2022
                     /locus_tag="JZ382_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY2428439.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="JZ382_002288"
     CDS             2013..2273
                     /locus_tag="JZ382_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428440.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="JZ382_002289"
     CDS             2434..2793
                     /locus_tag="JZ382_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428441.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="JZ382_002290"
     CDS             2790..4433
                     /locus_tag="JZ382_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY2428442.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..>5443
                     /locus_tag="JZ382_002291"
     CDS             4468..>5443
                     /locus_tag="JZ382_002291"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428443.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSI"
BASE COUNT         1941 a          749 c         1132 g         1621 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taa
//
LOCUS       DADJYG010000147         5375 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.153, whole genome shotgun sequence.
ACCESSION   DADJYG010000147 DADJYG010000000
VERSION     DADJYG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.153"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            154..1086
                     /locus_tag="JZ382_002292"
     CDS             154..1086
                     /locus_tag="JZ382_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428444.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1307..3070
                     /locus_tag="JZ382_002293"
     CDS             1307..3070
                     /locus_tag="JZ382_002293"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY2428445.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3154..3612
                     /locus_tag="JZ382_002294"
     CDS             3154..3612
                     /locus_tag="JZ382_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY2428446.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3721..4404
                     /locus_tag="JZ382_002295"
     CDS             3721..4404
                     /locus_tag="JZ382_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY2428447.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4551..5165
                     /locus_tag="JZ382_002296"
     CDS             4551..5165
                     /locus_tag="JZ382_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAY2428448.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5181..>5375
                     /locus_tag="JZ382_002297"
     CDS             5181..>5375
                     /locus_tag="JZ382_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428449.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1909 a          844 c         1108 g         1514 t
ORIGIN      
        1 ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat cccagaattt
       61 gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg ctatgaaaat
      121 aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga gactcatttt
      181 tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgaggaat agagatcgca
      241 atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag cgcagaaacg
      301 aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat tctagactta
      361 caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta taccaatgag
      421 aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt tctcaatgac
      481 ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga cgatgcagaa
      541 aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc agatggacaa
      601 gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct gtacggaaaa
      661 attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag atggaatgaa
      721 aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca agcggggaga
      781 caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt ttatcgaacc
      841 caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt atttcgttca
      901 ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt gtgcgatgct
      961 gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat ctcattaaaa
     1021 ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa aaaacatatt
     1081 tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc tgtagaaggt
     1141 ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt ctctgaaatt
     1201 tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata aagtgtaaca
     1261 aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga gtacagtatt
     1321 acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg ttgaggaaat
     1381 gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg atgtaggtgg
     1441 gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt atgtatttga
     1501 ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc ataagctttt
     1561 aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg gatattccaa
     1621 ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat ctatcagctt
     1681 agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg tggtagcatt
     1741 tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca atggtggtgt
     1801 tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg atgagaatca
     1861 tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa gcagcaataa
     1921 cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg atctggaaac
     1981 ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac ttcacgtcca
     2041 tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat atcattggca
     2101 tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag aaacatttaa
     2161 tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc ctattgccgc
     2221 agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa aacatccaga
     2281 tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt ctgctttggc
     2341 ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac gggcatatga
     2401 tcaattatca catgatttag cgatcaacga gaatccagtg attattttag tcggtggcgg
     2461 aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt acttatcaag
     2521 tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct ctatgctaga
     2581 ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac atggtgtaga
     2641 acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag taaataatgg
     2701 aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag aaacagtaaa
     2761 agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga tcacagaaaa
     2821 tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca cattagaaga
     2881 tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg attcttcaat
     2941 gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg tagggcagct
     3001 ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc gtgtcttgaa
     3061 tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta tctggatggt
     3121 tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga agaatatgtc
     3181 aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt cttttcattt
     3241 ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa gataaagact
     3301 ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc aaaagttggt
     3361 caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa aaatataaat
     3421 gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga aggagaaggg
     3481 gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca tgcttataaa
     3541 gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga atttgaagta
     3601 attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc tcctgaatag
     3661 aaaagggtta catttttata tgaagctgta tttttatata acattactgg aggagatatt
     3721 gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc tttggaaaac
     3781 gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa agaaggcgga
     3841 gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg aatcgttgat
     3901 ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt tattactgcg
     3961 acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc actggatgca
     4021 acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat gattcacgaa
     4081 cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca agcgcaagaa
     4141 gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc acgaaagacg
     4201 cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat cccagtaatt
     4261 atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt aggtgctttt
     4321 tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag atatgtcgaa
     4381 gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga caaaaaagaa
     4441 aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta cttttcattt
     4501 tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc atgtttgaac
     4561 ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt tcagtcatcc
     4621 ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat atcgcaccct
     4681 tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg atccagagaa
     4741 aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa gctgttatcc
     4801 atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat ctgcctaatg
     4861 aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc atttcagtac
     4921 cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat gtcgagatac
     4981 ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa tatcatttaa
     5041 atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg aatgctgtaa
     5101 gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt gcacgtcccg
     5161 aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca gattcaaaag
     5221 aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac ttagaagttc
     5281 cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag gaaatagaaa
     5341 tgccagatca aacgattagc ggattgttta attac
//
LOCUS       DADJYG010000148         5364 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.154, whole genome shotgun sequence.
ACCESSION   DADJYG010000148 DADJYG010000000
VERSION     DADJYG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.154"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(73..294)
                     /locus_tag="JZ382_002298"
     CDS             complement(73..294)
                     /locus_tag="JZ382_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428450.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(291..581)
                     /locus_tag="JZ382_002299"
     CDS             complement(291..581)
                     /locus_tag="JZ382_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428451.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(578..1762)
                     /locus_tag="JZ382_002300"
     CDS             complement(578..1762)
                     /locus_tag="JZ382_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAY2428452.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1944..3347)
                     /locus_tag="JZ382_002301"
     CDS             complement(1944..3347)
                     /locus_tag="JZ382_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAY2428453.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3369..4142)
                     /locus_tag="JZ382_002302"
     CDS             complement(3369..4142)
                     /locus_tag="JZ382_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428454.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4152..4529)
                     /locus_tag="JZ382_002303"
     CDS             complement(4152..4529)
                     /locus_tag="JZ382_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428455.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4550..4864)
                     /locus_tag="JZ382_002304"
     CDS             complement(4550..4864)
                     /locus_tag="JZ382_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428456.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5064..>5364)
                     /locus_tag="JZ382_002305"
     CDS             complement(5064..>5364)
                     /locus_tag="JZ382_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HAY2428457.1"
                     /translation="KKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFN
                     VSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1577 a         1067 c          916 g         1804 t
ORIGIN      
        1 catttgtaaa catattcata aatcctttca taacaaaata tatacttcct attttatcat
       61 gaaaaatatc tgttaagcac caatgattcg gttaaataag tttaataaca catctttcac
      121 gccagctgca ttgaacacaa gtccgacggc gattagagca ataatcaaga agccaatgag
      181 ttttgaaaat tctcgtttaa aacctaaaaa cagtccaatg acaacaatcg ctaacaatac
      241 caatgactgt gcattatcca agaaccagtt gtacaaattc tgcccaaaat tcatgtgatt
      301 gtcctccttt ccttaagata tttttctcga taatagatat agctttttac acgccttaat
      361 aattgaaaag gggttaaatg ctctccatag acggttactc gattggtata attattataa
      421 aatctcacct tgtaaaaatc ataggtatag atggtatcac cagcttgccg ttttccctca
      481 ataatgagag acgtatattt ccctgtattt tgtatatacg cttgaaactc aatgccacac
      541 tgaaacccta acttctttaa ttcttttgtt aaaaggctca tgtaatcaaa tcctccatgg
      601 ctagatgttg ttgttcaatt aatttctcat gtttttctgt cagtttggct tcctgaatca
      661 tggtggagat aatggttgta ccgttgagaa catctaaaat gcttgcgatt tttaatgtag
      721 gggcgacttg atgatgtaac cagttcaatg ttcgttcaaa agaataaggc tctggctgtg
      781 tggttaggcg taatgctcca cgatttttac cgataaacca ttcccaacgt tcattagttt
      841 tccaatcact ccgacgttta gtgtcgtctt tatctgcaaa gcggatatag cgattgataa
      901 tttcaaaggc tgttttttcc gcctgttgat gtgccaataa atctttcatg gcttgggtgg
      961 ctcggtcatt ttttaaccga atttcaaacc gattctttac ttctgcgtct tctaagggaa
     1021 catcattttt cacgtattgc tcataatctt tctcatatat acagaaatag acttcacttt
     1081 ttaatgagcc aatataaagt gtgtttccca tgtatggttt ttcatcacgg tgaaccaatt
     1141 caccactgcg ataattttta aagcttcgaa agacggaaat gcattcttcc tgtttacatt
     1201 tctttgccaa atctggaata ttcaaaatcc ctgttttatc gttgatagct aaatccaagc
     1261 gtttgaaaat acctttatgt tccatgcaat cttgaaagaa atcataccag ctccgatgtt
     1321 gagccaatag aaagttttca aattgccgac agccttttcc tttcagttct aataaaacac
     1381 ctttacttac atcatgagag actaggacaa caatatcacc gaaacgatag tgttcgggat
     1441 aagaataata gccaaagtct tcgtgtagca tataatccat attcaaacgt aaaacctcac
     1501 cgataatttg agccacatca ttggtaggga atcgaatccg tacatagtcg aataacatct
     1561 ccaatgggtt atcgggattg aagcgttcca aagcgttcag taaatcttct ttcacctgtt
     1621 gagtagggac tgccttgcct gtttcaatat ccgataggta aggtctggta atatgagaag
     1681 caaccgccag tttattttga gaaacgccat aagccagtcg cttttctttc aattccttaa
     1741 tccaaatttt ttcgttttcc attcgcaaac ctccaatcta aaaaagatgt aaactatgac
     1801 ccttgagttt acacctttca tattttccgc aatcctttgt accatcaaca ttggttgatg
     1861 agaattgcct gttttcagtt gtatttgtgc ccccctgtta gatagagggg gctaaaggat
     1921 tggcgtggct aacgccacac caatcaaagc aagtctgaac ctgtaccttt cacttcgtgc
     1981 ggtacaggct cagattgctt tttttgaatg acttttccta tttctcctaa gaaatcgtac
     2041 ctttttggta caaggggtgt ataaaactca ctaatcacac tttttcctgt atccacgtaa
     2101 cctcttccct tgatgggttt taagaaaaat tgtttttgtg tatcgcttcc gaacatcata
     2161 ccaaagccca tttcagacat acgacccaaa gccacacgga agttaaagtt gtcccgaatc
     2221 ccatcactaa aatattttgc gtctggacgt tgacaggcaa gaattaaaaa gaagcctgct
     2281 tgtcgtccta acattacaat ttgtttgagt ttatgaataa cggtggtact ttcacgacct
     2341 agcatatcca tcattgccac atattcatca aaaataagaa aattggcaga caaaccaaga
     2401 taagcataat tttctcctgt tttatagtta gggtgttgtt tcatggtttc actgcgatca
     2461 atcatatctt cgtaaaattg attgatacat gccaacatat cttctttacg gtaatagaca
     2521 ttattcatga ccgcccctaa gtctgccaaa tcagcatttt tagggtcaag aatataaagc
     2581 tttgcatttg tatgcaggag agattcaatc aatgtgagaa taaaatagga ttttccacta
     2641 ccagtgcccc cagcaattaa catgtgaggg agagaatcga actcccaata gaccgattcc
     2701 atcaatttga cacgtccatc ttgagctacc acgtcttcaa tgccaattcg attgccaata
     2761 gtatcataaa gtaggacata ctctacataa gaatctttca acgttttttc cactaattca
     2821 caatacaatc ctgtttctag tttgttttct aggtgcaaaa gctgatcttg atatttccct
     2881 aaggtgattt ctacatgaat atggattagt ccatctttca ttcgataata tattttagga
     2941 aaatggcgga ttcgttgttt ggttttcttg ctgggtaaat ctttaaagaa tccctctgat
     3001 tgagtttctt cggcttcata ccaatgattt tctaaaatca tacgagctaa cttttgacgg
     3061 tgctctaact gttttataga atctatacga tagtgataat aaagccagct gataagtccg
     3121 catacaaaaa gagcagtgag aatactgctc caaaagtatg gcgtatgaag ttgatagaaa
     3181 aatgtatcta actgaactgc ttgccagttc accgattgta acgctctcca atgaaaaggg
     3241 agtaggacga ctagaaaaac taccagcaat gaaattagta caaaatgata gaccaagtgc
     3301 ttatcgttgg ctttgattcg ctctcctctg tcatgaagca atggcataaa aatcctcctt
     3361 tccacctatc aaaataattg tgtattgtag atacgagcct gtttatcaaa agttggttga
     3421 gaaatggctt catatttctg ataagcgatt cgcttttcaa agcgtaaatt tttttctttc
     3481 ggataataat gacgtcgata gagagtgtgt cctaacagtt ttttcgcttc ttttgtcaac
     3541 ggatagagat agcgaaacat gcgtccattg attttctcaa tccctttgta ttcgcaaaat
     3601 gcctgggaaa gccaatgctt tttctctacc tgttcaaagc gagcattttc ctctaataaa
     3661 aggcgagcac ttcgagggtg tatcttttcc catgactgct tatcacgata gacacttgtc
     3721 ttgaaatacc cgcagtaaaa gaagttggac gcttgataga catagccaca ttttccctca
     3781 attccatctg ccagtgtata taaaaaatga cagtccgtat gttctttcaa ccacttaatt
     3841 agagctgaaa gagcttgact gccaaaataa ttggtttgat tcatttctgg aagaaaacac
     3901 atctttccaa tttctaaata atctgtggtt tgaagggaac tgtcgggaaa gagtttgcgt
     3961 atggtttgta ggggttgcgt accccagcct aattccacaa cgcctaataa tttttcttct
     4021 gaaaaaatcc ctagaaagta tttacacaat ctagggagta tctttgaata atggtattgc
     4081 cgaataaaag aaatggcttc atccttcgga atttctttca aaatcaatgg tgtgtttgtc
     4141 ataagcaaca cctatttttc actttcggac gcttcttttt tatatttctg atgaggtttt
     4201 gtctgtcctt ttaatacaag atcgtctgct ttcacgtacc aatcaacgtc tgcccctcga
     4261 aaagtagccg tcgctacggt atctgccaca ggattgatta attccacttc tgcgttgtag
     4321 tcaaattctt tcaaaggaac agaagcgggg atactgactt gtatcatgcg tccttgtcct
     4381 tttgatttta agtcataggt acgttcttta atttcttcgg aaaccgtacc atcttcattc
     4441 tggatacgaa cctcacgacg caaggcggaa aatttcaatg tgccaaatgt ctggtctttt
     4501 tcaatcacaa taccttctgc taatctcata ttttttcacc tttcctttct tatgctttca
     4561 ccatatcgtc tgcgtgcata atgtaattgg taaatcctcg attgccaatt ttataacctt
     4621 ctgcggtaat atgtgggttg atgagtttga ctttttcgtc ggattcaaaa tgtttctcgc
     4681 cagcttcgga gggaagaatg acaacaatat catctgctcg ttgaatcgtt gagtataaat
     4741 tgtaactacg agagagaatg gttggtctgc catttattcg acgttgttca atgttccctt
     4801 caccagcata ttctaattgt ccaaatgttt tttccatatt gggaatgaca tatttaagtt
     4861 ccatattttt acctatcctt tctgttttta tgtttgaatg aatcttattt gtgtgatggt
     4921 tttaaagggg ggagagcgac cttttgattc ttgatttttt attttcataa gttcacttcc
     4981 tttcaaaatt gggtaaaaaa atagacgctc cgtactgagc gtccgattcc tactatattt
     5041 aaaacaacga ctacctaggt attttaaata ttataatcaa attctatttt atctaataat
     5101 tgagtgggat aaagcaagtc tcctttactc gtaaaataac ccacctctga gaaaagaaat
     5161 aaattatttt ttgctcttga acaaattacg tatagtagtt ttctcgctga actaattcct
     5221 ttatcagaaa cattaaaaat ttcattccaa tgaggaattt tcccgtttag aagattaata
     5281 gcgataacag tatcatactc atttccttta actttatgtg ctgtagtaag tgtgatacca
     5341 tctcgagatt taaacatctt ctta
//
LOCUS       DADJYG010000149         5311 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.155, whole genome shotgun sequence.
ACCESSION   DADJYG010000149 DADJYG010000000
VERSION     DADJYG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.155"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(41..361)
                     /locus_tag="JZ382_002306"
     CDS             complement(41..361)
                     /locus_tag="JZ382_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAY2428458.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(509..2743)
                     /locus_tag="JZ382_002307"
     CDS             complement(509..2743)
                     /locus_tag="JZ382_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY2428459.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3222..3488
                     /locus_tag="JZ382_002308"
     CDS             3222..3488
                     /locus_tag="JZ382_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAY2428460.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3488..5215
                     /gene="ptsP"
                     /locus_tag="JZ382_002309"
     CDS             3488..5215
                     /gene="ptsP"
                     /locus_tag="JZ382_002309"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY2428461.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         1598 a          990 c         1068 g         1655 t
ORIGIN      
        1 ttttgtccca cgctctttaa ttattatgtt caaaatttgt ttacacggct aatgtagcgc
       61 ctaattcatg ttttggaatc gagatttcaa caaggcctgc caattcgatt actttgattt
      121 catcctcgtg gtaaacggcg tctttgattt tatctaagaa ataattttta gcatcttcga
      181 tttctttcgg ctggatttta cgtttgtcat tgatcagcag aatttcatta tgttcagggg
      241 cttcagtgta gacaatagtg gtatttccgg ctgtgtagac cttgactaga tgggcatcag
      301 tgttttcaag ctggtttaat acaagacggg aatggctgtt tgttacattt accaatttca
      361 tggcgctcac ctcttttatg tatttttaat tgcttgtttc tcttattata atttcttttt
      421 tataaaatga acagtatttt gacttttctg aaaaaagaat gagacggcat ttctcaaaga
      481 aataccgtct cacgctataa aactggattt attcagccgt ttcttcggct gattctttag
      541 ccaaacgttc tggtttagaa gtaacgatga tttttccatc tttatccagt tttgctttca
      601 actcatggat agaagggtga tcaagataaa attcagcgat accatcttcg atttgttctt
      661 ggatcgtgcg tcgaagagga cgagcaccca tagatggatc atagcctaag tcaactaatt
      721 tttctttgac attcgtagga acttcgatgt gaagttgctg tgctgccagc atttggttga
      781 catcgtctag cattagtgtg acgattgtca tcaagttttc ttttgataat gcactgaatt
      841 cgatgatccc gtcaaaacgg ttcagaaatt ctggtgtaaa gtagttgttt aattggttca
      901 atacagaacg agtaacacct tcgcgtgccg caccaaaacc aacgtttgct tctactttac
      961 cagttccggc attacttgtc ataatgatga tcgtatcttt aaagctgacc gttcttcctt
     1021 gtgcatctgt caatcgacca tcatctaaga tttgcaagaa catatgcaaa acatctggat
     1081 gagctttttc tacttcatct aacaagacaa gactgtaagg atttctccgt actttttctg
     1141 ttaattgacc agcttcttcg tatccgacat aacctggagg agaaccaatc aatttagaga
     1201 cactatgttt ttccatatat tcagacatat cgaaacgaat cattgaatct tgtgaaccga
     1261 atagttcgta ggcaagttgt ttcgcaagct ctgttttacc tacaccagtt ggtccaacaa
     1321 ataagaagga gccgattgga cgattctgtt tgtttaatcc aacacggttt cggcgaatcg
     1381 ctttcgctac tcgatctaca gcgttatctt gtccgatgac atgagctttc aagtcatctg
     1441 ctaagttttt cagttgtgtc tgttcttttt cttttaattc acccactgga atacctgtcc
     1501 gttgttcaac gattttttcc atatcttttt cggtaatcac aggtgtttcc tcttcagtga
     1561 gctgacgttc tttcattttt tgaagtttat tgatctgatc gcgataataa gctgcttttt
     1621 cgaaatcttc ttctcgagaa gctaaaactt tttgttcttc tgcttcttgc agttttttct
     1681 cgattgtttt aggatcaacg atttggatcg tcaagttttt cttagaacct gtttcatcta
     1741 gcaaatcgat ggctttatcc ggcaagaagc gatcttggac gtaacgattg gataaagttg
     1801 cggctgcttc gatggcctca tcagtatatt ttacatgatg atagtcttca taacgttttt
     1861 gcaaaccttt caaaatactg atcgtttctt cgacagttgg ttcatctacc cgtacaggtt
     1921 gcatacgacg ttctaaagca gcgtcttttt cgatgatgcg gtactcattt aaggtagtag
     1981 caccaaccat ttgcaattct cctcgagcta aagctggttt taaaatatta ccagcatcca
     2041 tatttccatc accagcagaa ccagcaccga cgatttcgtg aacttcatct atgaatagga
     2101 tcacattttc tgcttgacgg atctcttcaa tcaatttttg catacgttct tcaaattgtc
     2161 ctcgtatacc agtaccttgg actaaagata cgacatctaa acgaatgact tctttgtcca
     2221 ttaatttttg cggtacatca ccatctacga ttttttgtgc taaaccttca acaactgctg
     2281 ttttaccaac acctggttct ccaatcaaga caggattgtt cttggttcgg cgatttaaaa
     2341 tttcaatgac tcgtttgatt tcgtcatcgc gtccgatcac aggatcgata tcaccttgtc
     2401 gtgcttgttc tgtgatattg attccgtatt ctcccaataa accttctgta ttgccttgag
     2461 gtggctgacc accattgaag ttgttgtttc cgccaaattg tgttggtggt gtttgctcat
     2521 aatttccttg ctgttgcatt tggcgagaca tggaacggaa aagatcgtcc aaactgccaa
     2581 atccaaatgg atcattttgg gccatattag tcagacctcc attttgttga tttttgattt
     2641 tttgataaca gctctgacag taatcgagtt gtgtccgctg accattgaca gtggcataca
     2701 agtgaatcgt tgcctcattc ttgccgcagt tttgacaaag catgaatcat tcacgtcctt
     2761 tcacaaaaat gggtacatcc atattttaca tctcgttttt catgaggtaa aagaataacc
     2821 cctagttttt taagagattt atgaggtaga aaaggtgaaa tctctattga ccattcctga
     2881 cctttgactc ttattatact aaaaataaaa agggaagcaa gtaattcgtt tcatatgaaa
     2941 acaaagaggg tcaaaggaaa gaatgataaa cgtcaggaga agagaaagcg acaagaaaaa
     3001 ggaaataaac acgtttgtta taaaatgtga agaaaaggat gaaaaagaat ccggaataat
     3061 ttgaatactt ttatctgaaa ttgaatgaaa actgttgtat ctaacgaaaa aaaatgttaa
     3121 tctttacaga tgaaagggtg gcatttgtta ggattctctg aattactaaa aaagaaaacc
     3181 ctcacataaa aacaacactc acttaaagga gaccgattaa tatggaaaag aaagaatttc
     3241 acgtagtagc agaaactggg atccacgctc gtccagctac actattagta caaacagcaa
     3301 gcaaatttaa ctctgatgta aacttagagt ataaaggtaa atcagtaaac ttgaaatcaa
     3361 tcatgggcgt tatgtcttta ggtgtaggtc aaggttctga tgttactatt actgcagaag
     3421 gtgctgacga agctgatgca atggcagcaa tcgttgaaac aatgaagaaa gaaggcttat
     3481 ctgaataatg gttgaaatgc taaaagggat tgccgctagt gacggtgtag ccgttgcaaa
     3541 agcttacctg ctagttcaac cggatttaac attcagtaag gcaacagtag aagatactgc
     3601 ggctgaagaa gctcgtttgg atgctgcatt agctaaatct actgaagaat tgcagcaaat
     3661 tcgtgaaaaa gcagcgcaaa gcttaggtga agcagaagcg caagtatttg atgcccattt
     3721 gatggttctt tcagatccag aaatggtagg tcaaatcaag caaaatatta aagacaacag
     3781 cgtaaatgct gaatcagcgc taaaagaagt aacggatatg tatatcggta tgttcgaagc
     3841 aatggaagat aatgcttata tgcaagagcg tgctgcagat attcgtgacg ttgctaaacg
     3901 tattttagcg cacttattag gcgttacttt accaaatcca tcaatgatca acgaagaagt
     3961 ggtcgttgtt gcacatgact tgactccaag tgatacagca caattagatc gcaattttgt
     4021 aaaagcgttt gtaacagaca ttggtggacg tacttcacat tctgctatca tggcacgttc
     4081 tttagaaatt ccagcaatcg ttgggacaaa agaaattact gctaaagtca aagaaggcga
     4141 tattttggct gtcaacggga tcgaaggcga tgtaatcatc gatcctacag atgaacaaaa
     4201 agctgaattt gaaaaagcag gtgcagatta cgcagcacaa aaagcagaat gggaaaaatt
     4261 aaaaaatgca gaaactgtca cagctgacgg caagcatttt gaattagctg caaacatcgg
     4321 aacaccaaaa gatctagttg gtgtccataa taatggtgga gaagcagttg gtctgtaccg
     4381 tactgaattc ctgtatatgg attctccaga cttcccaaca gaagatgacc aatatgaagc
     4441 ttacaaagct gtattagaag gtatggaagg aaaacctgta gttgttcgta caatggacat
     4501 cggtggagat aaagaactac catatctgca attacctcat gaaatgaatc cattcttagg
     4561 atatcgtgca ttgcgtatca gtttatctga acaaggcgac gaaatgttcc gtacacaaat
     4621 gcgtgcattg cttcgtgcat ctgttcatgg taatttacgt atcatgttcc ctatggtagc
     4681 tacactcaaa gaattccgcg cagcaaaagc gatctttgaa gaagaaaaac aaaaattgat
     4741 aagtgaaggc aaggaagtat ctgatacgat ccaagtcggc atcatgatcg agattccagc
     4801 agcagcagtc ttagcagaca agtttgcaaa agaagtagat ttcttctctg taggtacgaa
     4861 cgatttgatt caatacacaa tggcagcaga ccgtatgaac gaacgcgttt cttacttgta
     4921 ccaaccatat aacccatcaa ttttacgttt gatcaaaaat gtaattgatg cagcacacgc
     4981 tgaaggaaaa tgggcaggta tgtgtggaga aatggctgga gatcaaacag ctgttccatt
     5041 gctagtaggt atgggtctag atgagttctc aatgagtgcg acatctattc ttaaaacgcg
     5101 tagtttaatg aaacgcttgg acacagcgaa aatggctgaa cttgctgatc gtgcattgaa
     5161 agaatgcgat atgatggaag aagtcgttga acttgttcat gaatatgtaa aataaacaac
     5221 gtatcaaaag ctggtgtggc ttgtaaaaga gctacgccag tttttttgtt cttttttaag
     5281 aaaacgtttt tgtcagtaag aatagacaaa a
//
LOCUS       DADJYG010000150         5160 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.156, whole genome shotgun sequence.
ACCESSION   DADJYG010000150 DADJYG010000000
VERSION     DADJYG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.156"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..152
                     /locus_tag="JZ382_002310"
     CDS             <1..152
                     /locus_tag="JZ382_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428462.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="JZ382_002311"
     CDS             complement(230..763)
                     /locus_tag="JZ382_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAY2428463.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="JZ382_002312"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="JZ382_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY2428464.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="JZ382_002313"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="JZ382_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY2428465.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="JZ382_002314"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="JZ382_002314"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY2428466.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="JZ382_002315"
     CDS             complement(4488..5066)
                     /locus_tag="JZ382_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAY2428467.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1490 a         1053 c          923 g         1694 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
//
LOCUS       DADJYG010000151         5105 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.157, whole genome shotgun sequence.
ACCESSION   DADJYG010000151 DADJYG010000000
VERSION     DADJYG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.157"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            68..2002
                     /locus_tag="JZ382_002316"
     CDS             68..2002
                     /locus_tag="JZ382_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY2428468.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2459..2875
                     /locus_tag="JZ382_002317"
     CDS             2459..2875
                     /locus_tag="JZ382_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAY2428469.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2889..3455
                     /locus_tag="JZ382_002318"
     CDS             2889..3455
                     /locus_tag="JZ382_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAY2428470.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3478..4464
                     /locus_tag="JZ382_002319"
     CDS             3478..4464
                     /locus_tag="JZ382_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAY2428471.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4619..>5105)
                     /gene="scm"
                     /locus_tag="JZ382_002320"
     CDS             complement(4619..>5105)
                     /gene="scm"
                     /locus_tag="JZ382_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAY2428472.1"
                     /translation="TSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSD
                     SSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSS
                     DNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSK
                     R"
BASE COUNT         1544 a          945 c         1124 g         1492 t
ORIGIN      
        1 taagtatcaa actaaaagaa ttatcatata ttcattatat agatagtcta taaggagcgg
       61 atgatcaatg gcaaagcaaa atattgtcgt tgtaggagca ggatatgccg gtgtttcagc
      121 tacaaaatac ttggcaaaga aactgaaaaa agaagatgta gcaattactc tgatcgatcg
      181 tcattcgtac catactatga tgaccgagtt gcatgaagta gctggcggac gtgtagaacc
      241 agaagcaatc caatacgatc tgcagcattt gtttgctcgt caaaagaatg tacaacttgt
      301 cacagacact gtaatcggta tcgataaaga acgaaaaatc gtcaagacca aattaggttc
      361 ctatcctttt gatcagctga tccttggaat gggcggagag ccgaatgact ttggtacacc
      421 tggtgtcaaa gcacacggat tcaccctttg gtcatttgaa gattctgtaa ggattcggga
      481 acatattgag aagacagtag caaaagctgc attggaacca gatgatgaac tgcgtaaagc
      541 catgctgact ttcgttgtct gtggttctgg atttaccggt attgaaatga ttggggaact
      601 gatcgactgg aaaaatcgcc tagccaaaga ttataaatta gatccaaacg aaatcacact
      661 gatggtcgta gaggcaatgc cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa
      721 agctgaacgc tatttgcgta agcacaacgt ggaattatta ttgaatgcac caatcgtaga
      781 agtggctgct gatcatatca aattgaaaga cggacgcaac attccgactc acacactgat
      841 ttggacagct ggtgtcaaag gaacttctga cgctgctgac tttggattag aatcagcacg
      901 cggacagcgg ttgatcgcca atgaatatat gcaggcaaaa ggctacgaag ataaaaacat
      961 ctatatcatt ggtgatttag tcttttatga agaattccca ggaacaccga caccgcagat
     1021 cgttcaagct gctgaacaaa caggacacac agccgcagcg aatatcgtgg cttcgatcaa
     1081 aggcggagaa aagcataaat ttaaaggaaa ctatcaagga tttatggttt ctattggtgc
     1141 caaatggggg gtagctaacc tatttgataa gatccattta agcggatttt tagctatcgt
     1201 aatgaaacat atcgtgaatt tgaaatactt ctttgatatt cgttctggtt attatatgtt
     1261 ccaatattta atgcatgagt ttttccatat caaagatgat cgcaacgtga ctcgaggaca
     1321 ttcttctcgt tacggcaatg tcttatggag tgtgccactt cgtattttct atggtttagt
     1381 ttggttcgtt gaagcttcca aaaaaatcat tgggaatggt gaagtgttca aaccaagtac
     1441 ttggtttggt gaaggatcat ggttcactgg aaacagtgtg ttcccttgga catgggaata
     1501 caatgtaaca gatgttgcca gtggtgcttc tgctgctggt gatgcaacca cagctgccag
     1561 cggagcaggg gcaagtgccg gagaagcagc gactcaagct gcacattttg gtttgagtta
     1621 tgcatacggt gaagaaccaa tggctgtgat tgagaagtca ccagactggt tcacaaagat
     1681 catgcaagtg atcatgccaa acaatgatgt agcaatgttc ttccaaaaat tcatggtatt
     1741 ctttgaaatt gccttggcat tagcattgat tgctggatta ttcacatggc tatgcagtgc
     1801 gacaacgatt gtattggtga tcacgttctg cttatcaggc atgttttact gggtaaacat
     1861 ttggttcatt tttgttgctt tcgcattgat gaacggttcc ggacgtgcga ttgggttgga
     1921 tcgctgggta attccatggc tacaacgtac actaggtaat tggtggtacg gtagaccaag
     1981 ttcccagtac ggcaagaaat aaatagataa agaacgttat ttgaatgcaa gatttcaata
     2041 gaaaggggct gagacaaacc gtcacagcct cctttttatg taaaattaaa ggaataagat
     2101 gcccgagata agcctccatg agcaagacac aggtgtcaca acctctgtta caacctcttg
     2161 ttataaacgg tgttccagaa gtagcttctt cgaaaaataa gccgaaattt cacaaaaact
     2221 tgaaaagtaa ttttcgtaaa ttccttctta tttcttgaag ttgcccactt ctgtcacaac
     2281 ctcttgttat aaacggtgtt ccacctgctg cttctcggta ataagcccaa agaattagaa
     2341 aacatggaaa gctgttttct attcttcgtt cttattactc aaagcaagac acaggtgtca
     2401 caacctcttg tttttatgct aaaattgggt gagttaatct ggaaaaagga gaacaaaaat
     2461 gagccttaaa gaatttgctg aaaaaagtct gctcaaacct tgggatgttt ttattatttt
     2521 gcttttgact gttctttctt ttttaccagt catgattttt ggtcttcagc aaaacaccgc
     2581 aacagcagaa aaagaagcgg tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt
     2641 gatggctgga gaagagagca acacatatac ttacaaagat gaacacggag attataactt
     2701 gatcgagata gatggcgatc gagtacggat aaaagaagca gattgcgatg accagatctg
     2761 cgtacgtcga ggctgggcaa cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt
     2821 agtgatcgaa gtccgttcaa cagatggagg tgacgaagac agtctgattt attagacctt
     2881 cacacaccat gagtaaatta caaaaaatga tttatatttc gctacttgtt gcacaaggag
     2941 tgatcattgg tttattggaa aatatgatcc cttatccttt ttcttttgcg cctggtgcaa
     3001 aactaggctt agccaatcta atcacgatcg ttgccatttt taccatgaaa aaacaagaca
     3061 gcttcttact actttggcta cgactattcc tgactacttt gctaggcgga acgatttcca
     3121 cattccttta tagtatgagt ggtgctttgc ttagttattt tggaatgtta ttggtcaagc
     3181 aattaggacc aaaacgtgtc agtatcatcg gaattagtgc ggccggcggt ttcatgcata
     3241 atgtcggaca gcttttgatg gcttctttca ttgcacaatc atggacagtc atgttgtact
     3301 tgccagtcct ttcagtttta gggattttat cagggattgc aatcgggatc gcagcgaatt
     3361 atcttttacg acatgtgcag acacttcaac gattccaaaa agattatgag cagggaacca
     3421 aacaaaaatg gagcccaatg tttaccaaaa aataatcgtc acacaaggag tttttttatg
     3481 aagctacatc caatgtggga aaactatcct gctttaagta aagaattgac agaaacacta
     3541 aaacggatgg aagaaactgt ccgcctgaaa aacaaacctg tcgaacaagc catcaaagaa
     3601 acgatccatg ccggcggaaa gctgttgcgt cctgcctatc agctattgtt ctcacaattt
     3661 ggaccagagc aagatcgaga caaagccatc gctttggcag cttctatcga aatgctccat
     3721 acggccacac tgatccatga tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct
     3781 aatatccgta gtcagtttgg taatgtcgtt tctgtctatg caggagacta tttatttgtc
     3841 tgctgtttta aactattgtc tgattactcc acttccttga aaagtctcca attgaattct
     3901 cgcagtatgg aaaaagtatt gaatggcgaa ttagggcaga tggatgatcg ctataattat
     3961 gaactttctg taaaagaata tttggaaaat atttcaggta agacagctga actgttccaa
     4021 ttaagttgtt ctgttggcgc ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca
     4081 ggagatatcg gactttctat cgggatggcc tttcagatta tcgatgatat tttagattat
     4141 acaaaagaga gccaggaaat cggcaaacct gtactagaag acatgcgtca aggggtctat
     4201 tctttaccgt tgatctactc tttgcaaaaa aacaaaccaa agctactacc ttatttagag
     4261 aagaaagcgg caatgacaga agaagatgtc gacgccgtac gcaaaatcgt tgaacataca
     4321 ggaggcgtgg aagaagctag gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa
     4381 ataaagaaat tacctgccac ctctcttcga acaaaagaga atctcttttc ccttacccaa
     4441 ctgattttgg atcgcaaaga ctgaataggg aaaggttcga tacgtaaata aatttctcag
     4501 aaggaaatct acgtgttttt tgacaaattg aattgtagtt cttgtattta tgataaaaag
     4561 caaaaataaa gagcaacgtc cgttagcgac gttgctcttt tttatcttga atattttttt
     4621 accttttcga tttcttataa ctcataaaag tgattcttaa accgattaca agtaatataa
     4681 cgcctgcgag tgttatccaa ttattcgatt gacttccagt tttaggaagt gctctttttg
     4741 atttgtttcc attgtcattg cttgttttat tagttaagtt aaagttcgtc ctgttatccg
     4801 aacttgtttt tgagaaggag acacttgcat gacgtgtatc gcttgtggta gacgacgtgt
     4861 ctgtactatc gcttgaagtt gatgaatctg agctatcact tgtggtagag gatgtatctg
     4921 tactgtcact tgaagctgac gtgtccgtgc tatcacttgt agtggtagat gaatctgagc
     4981 tatcacttgt ggtagaggta gtatctgtac tgtcacttga agctgacgtg tccgtgctat
     5041 cacttgttgt ggtagatgta tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac
     5101 ttgtg
//
LOCUS       DADJYG010000152         5075 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.158, whole genome shotgun sequence.
ACCESSION   DADJYG010000152 DADJYG010000000
VERSION     DADJYG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.158"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            49..1217
                     /locus_tag="JZ382_002321"
                     /pseudo
     CDS             49..1217
                     /locus_tag="JZ382_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1343..2422)
                     /locus_tag="JZ382_002322"
     CDS             complement(1343..2422)
                     /locus_tag="JZ382_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY2428473.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2525..2965)
                     /locus_tag="JZ382_002323"
     CDS             complement(2525..2965)
                     /locus_tag="JZ382_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2428474.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3022..3432)
                     /locus_tag="JZ382_002324"
     CDS             complement(3022..3432)
                     /locus_tag="JZ382_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAY2428475.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3612..4046)
                     /locus_tag="JZ382_002325"
     CDS             complement(3612..4046)
                     /locus_tag="JZ382_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY2428476.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4146..4994)
                     /locus_tag="JZ382_002326"
     CDS             complement(4146..4994)
                     /locus_tag="JZ382_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAY2428477.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         1848 a          976 c          849 g         1402 t
ORIGIN      
        1 ctactatctt atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca
       61 accatcttaa tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc
      121 cacacagagg agatagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga
      181 attacctttc aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga
      241 caaacagcga ccatcaaagc aaaaattgac tattcaggag acttagcacc tatactacaa
      301 tcagctgtta catttgagaa tgttgatccc gctttgtcga ttggctatga cgagaacact
      361 ttgactatcc aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg
      421 ttaaaaccag cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcatcat
      481 ttaacgccgt atagtcagcg gcagacaatt gagcaagatt cagctatcga ctcaggagga
      541 gatcttgttg aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct
      601 acagaaaatc cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca
      661 gaaactcaac cagagccaga caatgaatca aaacctgaaa taacccctgg agaaaaacca
      721 gatgtagacc cagaagaaaa accagatgtg actccagaac cagatacgga ttctggaaac
      781 caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc agaaaaacca
      841 gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga cgcaagagac
      901 caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa aaaagagagt
      961 aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa aaaatcacga
     1021 atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac aacctttgct
     1081 gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac aaagttatac
     1141 tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa agatttaaac
     1201 aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa ataaaaagaa
     1261 atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg tcgttcatac
     1321 tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga tggttcggga
     1381 tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg acaatcaagc
     1441 cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa aaaatcggca
     1501 gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt agatggacaa
     1561 tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa taagctatga
     1621 aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt aaaatgaaaa
     1681 tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg attgtagtga
     1741 gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca aatgtattga
     1801 caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca ctttttaaaa
     1861 acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa cttaagatga
     1921 atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca agcgcaatcg
     1981 aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta tggatatatt
     2041 gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg gactggatca
     2101 tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga tagataaggt
     2161 aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg acaaatcgct
     2221 gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc aaaatggttg
     2281 taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca gtgagacggc
     2341 gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc tccaaataac
     2401 aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag agcaaaaaga
     2461 aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg ttcatctagc
     2521 agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg gtcagatgtt
     2581 gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt tgtggaatac
     2641 cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt acgattcttt
     2701 catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt tttagtaagg
     2761 gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact gaagtctttt
     2821 tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc agcggcttga
     2881 ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga aaacatactt
     2941 gattaactaa atatagatct tccatttttc cacctccgct gttttactat agtgtatcat
     3001 agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt ctatatttcc
     3061 agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt cttgcgtttc
     3121 ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa agctcgtttc
     3181 actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga tatttttttg
     3241 ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg caaacaattg
     3301 ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca attcgaatgt
     3361 acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt tcgtagaata
     3421 catttttttc ataaaattac cacctttaat tatattttat acaattgaat tttacacaat
     3481 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
     3541 cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct
     3601 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca
     3661 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
     3721 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
     3781 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
     3841 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta
     3901 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
     3961 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
     4021 aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
     4081 tagattcttt tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta
     4141 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
     4201 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
     4261 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
     4321 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
     4381 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
     4441 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
     4501 ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
     4561 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
     4621 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
     4681 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag
     4741 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
     4801 ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt
     4861 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
     4921 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
     4981 ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat cttattataa
     5041 cttacttttc accttaagtt aactttaatt aataa
//
LOCUS       DADJYG010000153         5005 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.159, whole genome shotgun sequence.
ACCESSION   DADJYG010000153 DADJYG010000000
VERSION     DADJYG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.159"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..182)
                     /locus_tag="JZ382_002327"
     CDS             complement(<1..182)
                     /locus_tag="JZ382_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2428478.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="JZ382_002328"
     CDS             517..951
                     /locus_tag="JZ382_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAY2428479.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="JZ382_002329"
     CDS             974..1294
                     /locus_tag="JZ382_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428480.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="JZ382_002330"
     CDS             1435..1626
                     /locus_tag="JZ382_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428481.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="JZ382_002331"
     CDS             complement(1697..1954)
                     /locus_tag="JZ382_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAY2428482.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="JZ382_002332"
     CDS             2112..2375
                     /locus_tag="JZ382_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428483.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="JZ382_002333"
     CDS             2399..2587
                     /locus_tag="JZ382_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428484.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="JZ382_002334"
     CDS             complement(2670..2855)
                     /locus_tag="JZ382_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428485.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="JZ382_002335"
     CDS             3070..3441
                     /locus_tag="JZ382_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAY2428486.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="JZ382_002336"
     tRNA            complement(3591..3663)
                     /locus_tag="JZ382_002336"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="JZ382_002337"
     CDS             complement(3709..4137)
                     /locus_tag="JZ382_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428487.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="JZ382_002338"
     CDS             complement(4197..4955)
                     /locus_tag="JZ382_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAY2428488.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1743 a          784 c          978 g         1500 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttt
//
LOCUS       DADJYG010000154         5005 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.160, whole genome shotgun sequence.
ACCESSION   DADJYG010000154 DADJYG010000000
VERSION     DADJYG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.160"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(4..1029)
                     /locus_tag="JZ382_002339"
     CDS             complement(4..1029)
                     /locus_tag="JZ382_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2428489.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="JZ382_002340"
     CDS             complement(1274..2545)
                     /locus_tag="JZ382_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAY2428490.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="JZ382_002341"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="JZ382_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="JZ382_002342"
     CDS             3788..4261
                     /locus_tag="JZ382_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2428491.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="JZ382_002343"
     CDS             complement(4434..4988)
                     /locus_tag="JZ382_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2428492.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1581 a          954 c          743 g         1727 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tctta
//
LOCUS       DADJYG010000155         4973 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.161, whole genome shotgun sequence.
ACCESSION   DADJYG010000155 DADJYG010000000
VERSION     DADJYG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.161"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..939
                     /locus_tag="JZ382_002344"
     CDS             <1..939
                     /locus_tag="JZ382_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2428493.1"
                     /translation="EWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLA
                     IDILTFLTGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHI
                     ESFVDYDFAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAM
                     LIVISTFALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVT
                     QNADDLLDEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCL
                     VSDIYGRVGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            936..2963
                     /locus_tag="JZ382_002345"
     CDS             936..2963
                     /locus_tag="JZ382_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428494.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            2960..3982
                     /locus_tag="JZ382_002346"
     CDS             2960..3982
                     /locus_tag="JZ382_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAY2428495.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            3999..4928
                     /locus_tag="JZ382_002347"
     CDS             3999..4928
                     /locus_tag="JZ382_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428496.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         1636 a          903 c         1023 g         1411 t
ORIGIN      
        1 gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa tctcacgagt
       61 gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc gaaagattca
      121 gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg agatagcgag
      181 aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca acgagggttg
      241 ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag tattgctgac
      301 catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga tggcagtgtc
      361 acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga tttggtctta
      421 cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag tgtggcaatg
      481 ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc catttttaaa
      541 attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa agctctatct
      601 atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt tactcaaaac
      661 gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt tgcctttcgt
      721 tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga caaggaagat
      781 gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt ttcagacatc
      841 tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct tcatgccttt
      901 gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa aagaaatttc
      961 ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta ttaggcacgc
     1021 tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca agcaatgaat
     1081 actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat agctggggct
     1141 ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga ttgtatgcca
     1201 ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt tatttggtga
     1261 aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc gaaaacatgc
     1321 agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat gtcggtttcc
     1381 tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg gttaagcgag
     1441 aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt ctatcggctt
     1501 cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc aaagatgtca
     1561 gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat agtcaaggga
     1621 aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag caaccatggc
     1681 tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt gtggaaagtt
     1741 tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta gtgattgaag
     1801 aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc agattaggaa
     1861 cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc cttctaacag
     1921 gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg ccaatcagtt
     1981 tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt accaagttat
     2041 ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc tttagtattt
     2101 ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg tttttacaaa
     2161 ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta ttcactttac
     2221 aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt caaatgatga
     2281 atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga aaagcagggt
     2341 caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct aaccatactc
     2401 gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg aaaaagcaac
     2461 aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac acgaaaaatc
     2521 gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca acccatgtca
     2581 aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac tttaaacaat
     2641 ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag gagcaaaaac
     2701 gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa agacgaaaag
     2761 aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt cagcaaaccg
     2821 tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca ttgaaaaaag
     2881 agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat aaaagaacgg
     2941 ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg tggtcttctc
     3001 tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata cggacagtgg
     3061 cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac cagaagtgtt
     3121 ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag actatgtttc
     3181 ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg ttatgcaaag
     3241 ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca tcaaacaagg
     3301 ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg atatagattc
     3361 tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga gtcatgggaa
     3421 aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg gtcaaaaagc
     3481 agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata actatggaaa
     3541 tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa cagaatttga
     3601 tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt ttccctatgt
     3661 ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac aatgggtgta
     3721 tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg ctacgcaaga
     3781 aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa cctataatgc
     3841 aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt atcatgcagg
     3901 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     3961 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     4021 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     4081 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     4141 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     4201 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     4261 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     4321 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     4381 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     4441 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     4501 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     4561 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     4621 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     4681 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     4741 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     4801 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     4861 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     4921 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tct
//
LOCUS       DADJYG010000156         4931 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.162, whole genome shotgun sequence.
ACCESSION   DADJYG010000156 DADJYG010000000
VERSION     DADJYG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.162"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(92..1240)
                     /gene="nagA"
                     /locus_tag="JZ382_002348"
     CDS             complement(92..1240)
                     /gene="nagA"
                     /locus_tag="JZ382_002348"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY2428497.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1316..2152)
                     /locus_tag="JZ382_002349"
     CDS             complement(1316..2152)
                     /locus_tag="JZ382_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428498.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2336..3553)
                     /locus_tag="JZ382_002350"
     CDS             complement(2336..3553)
                     /locus_tag="JZ382_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY2428499.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3936..4019
                     /locus_tag="JZ382_002351"
     tRNA            3936..4019
                     /locus_tag="JZ382_002351"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3970..3972,aa:Leu,seq:gag)
     gene            complement(4259..4837)
                     /locus_tag="JZ382_002352"
     CDS             complement(4259..4837)
                     /locus_tag="JZ382_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428500.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1514 a         1016 c          789 g         1612 t
ORIGIN      
        1 tgaaacttat gtaaagtgat ttttactaga acacaaaaaa accttaaaac caaacttaag
       61 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
      121 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      181 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      241 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
      301 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      361 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      421 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      481 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      541 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      601 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
      661 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      721 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
      781 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      841 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      901 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      961 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
     1021 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1081 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1141 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1201 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1261 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1321 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1381 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1441 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1501 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1561 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
     1621 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
     1681 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1741 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1801 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1861 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1921 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1981 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     2041 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
     2101 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
     2161 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2221 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2281 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2341 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2401 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2461 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2521 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2581 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2641 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2701 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2761 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2821 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
     2881 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2941 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     3001 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3061 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3121 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3181 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3241 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3301 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3361 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3421 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3481 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3541 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3601 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3661 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
     3721 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3781 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3841 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3901 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3961 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     4021 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4081 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4141 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4201 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
     4261 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4321 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
     4381 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4441 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4501 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4561 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4621 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4681 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4741 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4801 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4861 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4921 atttattaat t
//
LOCUS       DADJYG010000157         4895 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.163, whole genome shotgun sequence.
ACCESSION   DADJYG010000157 DADJYG010000000
VERSION     DADJYG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.163"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(406..780)
                     /locus_tag="JZ382_002353"
     CDS             complement(406..780)
                     /locus_tag="JZ382_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY2428501.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="JZ382_002354"
     CDS             complement(803..2203)
                     /locus_tag="JZ382_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAY2428502.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="JZ382_002355"
     CDS             complement(2207..2617)
                     /locus_tag="JZ382_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY2428503.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="JZ382_002356"
     CDS             complement(2635..3126)
                     /locus_tag="JZ382_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY2428504.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="JZ382_002357"
     CDS             complement(3136..3927)
                     /locus_tag="JZ382_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY2428505.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="JZ382_002358"
     CDS             complement(3920..4693)
                     /locus_tag="JZ382_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2428506.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1556 a         1000 c          647 g         1692 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctc
//
LOCUS       DADJYG010000158         4892 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.164, whole genome shotgun sequence.
ACCESSION   DADJYG010000158 DADJYG010000000
VERSION     DADJYG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.164"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1022..1954
                     /locus_tag="JZ382_002359"
     CDS             1022..1954
                     /locus_tag="JZ382_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428507.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            1971..2486
                     /locus_tag="JZ382_002360"
     CDS             1971..2486
                     /locus_tag="JZ382_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAY2428508.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            2782..3063
                     /locus_tag="JZ382_002361"
     CDS             2782..3063
                     /locus_tag="JZ382_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428509.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            3065..3481
                     /locus_tag="JZ382_002362"
     CDS             3065..3481
                     /locus_tag="JZ382_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428510.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            4138..>4892
                     /locus_tag="JZ382_002363"
     CDS             4138..>4892
                     /locus_tag="JZ382_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAY2428511.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVR"
BASE COUNT         1881 a          813 c          785 g         1413 t
ORIGIN      
        1 ggagcgtgag ttttgagggg gattcaaggg ggagtttctc ccctttgaca agcggttatt
       61 tgcaccaaat acgaaggtat ttggctagca aatatagagc ttgccaaacc ctttagtgtg
      121 ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc ctatggacgt
      181 atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa tccttctaaa
      241 aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga atccgaattt
      301 gcaaatctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag gtaacattag
      361 tcgatatata cataaggctc taatttgcgt tttaaggcgt tttaaacgtt ctggtatgta
      421 atttatacca aagcgacttt aaaacgcctt aaaacgaatt ttataggtct tgtaggacgt
      481 ttcagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac aagataaggg
      541 attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc ttaaaggact
      601 ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttc aatgtgagag
      661 cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta ggaaatttcg
      721 cgaacctgtt tggagaaaaa aagtaggaaa tttttaggcg ggaaattttg cccccctctt
      781 ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta tccttactct
      841 cccaaggcct gtagcttttt tagggcatga aattatctgt ttataggcat gaaattatct
      901 gtttataggc atgaaattat ctgtttatag gcatgaaatt atctgtttta ggtattgttt
      961 taagacataa tataccttaa aattatgtca ataaaattag ttagactgga gtatcaaata
     1021 aatggcaaat gaaatcgtaa aatataaacc agaattaaat accattcctt taaggaaatt
     1081 tactccaata gaaatgaact tgtttttttc tattgtttct agaatgcgtg ataaaggtaa
     1141 tcaaaaaata cgcttttatt tcgatcagct taaagattta agtgcttata agcaaactgc
     1201 tggagaagtc tttataaaag atttaacaag aacatacgat catttaatgg atttacgttt
     1261 tggaaaaaaa agcaatggtg gattaaccat agaacgattt acaatgttta ctgattttga
     1321 aattcatgga gaagccgata acccttatgt ggatataaaa gttcacgaaa aagctttgcc
     1381 tttacttaac aatttagaaa gttgggtacg tttctcttta acagaatttc gagatttaaa
     1441 tagcagttat gctaaaacaa tgtttagatt actaaaacaa taccgaacac aaggatgggc
     1501 tgaattttcc aaggaagatt ttttagaact attagatatt cctaaaagct atagacaaac
     1561 aaatataaat cagtttgtac tcaaacccat aaaagaagaa ctcacaccat tatttaaagg
     1621 attaacgatt cgtaaaaaat atggtaaagg ccgaggcaaa cctgttattg gttatcgttt
     1681 tacttggaaa gctgaaatta atcatgctga cgatttttca aaagggaagc aagaagattt
     1741 acgtacaaaa ttatttaata ttgaacacaa tggagaatta acgcaagaag aaaaatggcg
     1801 cgctaaagat cgaatactag atcttccgtt aggtactcat gaagctaatt ttaaaaaaca
     1861 acaacaaact gaaaaagaag aagtggaaaa acaaacactt tcaaacgaac ttaaacaaga
     1921 tttattagga aatttacaaa acttattcga ttaaagtata aggagtattt atggcagata
     1981 aaacgatcaa agaccttgct gaagaattag gagttagtaa gacagctatt aacaaaaaag
     2041 tttctgacga aaatcgaaaa ctttggtttg cgaaaatcgg aaacaagttt gtgattaacg
     2101 aaatcggtca aaaagctata aaatcaatgt tttttaccga aaacgaaaac tcaaatcaac
     2161 aaccagttgg cgaaaaatcg aaaaccgaaa accataaaaa cgccaaccaa gtttccgaat
     2221 tagaactctt ttttttccta gaaaaacaat taaatcaaaa agatatacag ctacaagaaa
     2281 aagacgaaca attaaaacaa atgcaaaaat tactcgacca gcaacaacaa ctcaccttac
     2341 aagccaatca acaaatcaaa aaacttcacg aacaactcca gctaacctat gcagaagaat
     2401 cttcagaaaa agaacccctt tctgttactg aagaaactca aaaaggacaa aacaaggaga
     2461 aaaaatggtg gcaattctgg caataaccca acaattttta ttcataaaaa ttgttgggtt
     2521 actttctttt tttgctttta caagcatagc gacatgatct cacgtcgcta ttttttaaag
     2581 agcataagga aacgcggtat cactttctta cgaaaggtgc taggtaggct gtgcctacca
     2641 cgtttctaag aaaaaaattt gctatcactt tttcgccatt gtggctagct tatgtatagt
     2701 atttcggtaa aaaacagcta tacaaatact atatttgtat gatatacata aattatacac
     2761 acataagaaa ggacgttttc tatggacatt ttagttcgaa atatcaatcc aaaaattatt
     2821 acaagagtag ataaacgttg tcaggaactt acaaccaaaa caggagaaaa atggagtcga
     2881 aataaatatt taaaagtgct aatcgaaagt gattttgacc gtcaacttat gaattacaaa
     2941 aaagagcaat tcgatttatt actcgaaaaa tttatcgata ttcaaacaaa aaatactgaa
     3001 acattgaacg actatgttca tacaaacaac caactcattg aaacacttat cggaggaata
     3061 taacatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acattgacgt
     3121 tttagattcc atcatcgaaa aaaatccaga tatcaataac tactcggaag ccattcgctt
     3181 tttatgtatg agttatgaac ctgataccaa tacaacaact accagtaacc aagccaaact
     3241 aaatgcgatg ggaaaagaaa tttctatcat gaccgaaatt atcgcagaat ttgctgattt
     3301 gtatttaaaa gatacaggcg ttcttatcgg tacaaactcg aaagtatatc aagaagtaaa
     3361 agaaaaaatt gaaaaacagc taaatccctc ccaacgaaat caaccaaaga atcgtcaaaa
     3421 agaaacgagt caaacattca cttctaaaaa tttcgaccca accaactttc taaaaagata
     3481 aaaaaataaa ccaggaaatt tttaatttcc tggtttattt tctagagagc ggtcttccaa
     3541 taagatttgc aaatcgatcc caatttgcgt aaaaaaaaag ctgctcgtac gagcagctaa
     3601 acaagaaaaa gtcagcttgt tttaagggta aaataagtgt aacaaatttt gcgaatgcaa
     3661 cgatcttttt tattatacgc aaacaatcaa aaaaaaagac tgctcgaaag agcagtctca
     3721 agcaaaaaat aaacgaacaa acgcaattac tcacgaacac aaacaaatac aataaaatac
     3781 ctaaataatg aaaataagtg ataaaaaaca agggtattta tagtttaaca agcgctcata
     3841 aaaataacaa gtctttaaaa aatgacagat aaaaatgcac aaagaatcgg acacagataa
     3901 ccgcgaaaaa tcaagcttta gaaagttgtt tttagctaga ccaatgaaaa atcacaaagt
     3961 ccgcccctgt atccgttggt agaggggcta cttggtttag tatacattat gttaactagc
     4021 tcgttaaata acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt
     4081 atcgcatatt gtatatttta attataggtg tatatcactt aattttaaca caaaggcgtg
     4141 attattttga aacccaaaaa taaaatttat ctaggtctcc tcttttcctt gatgattgga
     4201 atttttagta taggaattac aaatacctat gcttcagaaa attctaattt caatatcaac
     4261 tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa ctgattttga acactccata
     4321 acttcaattc ttttaatgga aaataacact gaaactgata ttaactatac tcttggttcg
     4381 ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt ctattttctt tccagtattt
     4441 tcagacgctg ataatacgat taaatatgtt tataccgtca aaaaaaatac aaatggagaa
     4501 tattcaggaa gtatttcaca atttttagca aaagaacttc aaaatctagt agatagcaat
     4561 ataacagata caaccttatt ttttcaagat aatagcattt attattccga taatggccaa
     4621 attaaactac tttggaaatc acctttaagt acagaaaaaa atggtgtctc ttcctttaca
     4681 gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa ttaaaaattt ctctaaaaaa
     4741 caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct ccggagataa ctttattatg
     4801 attaattggc gtatagcaga aacccaaaca agttttccgt ggtgcgctgc ttacgtagaa
     4861 gctgctatct tatgtaataa aacagatgta cg
//
LOCUS       DADJYG010000159         4846 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.165, whole genome shotgun sequence.
ACCESSION   DADJYG010000159 DADJYG010000000
VERSION     DADJYG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.165"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(2..262)
                     /locus_tag="JZ382_002364"
     CDS             complement(2..262)
                     /locus_tag="JZ382_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428512.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            337..540
                     /locus_tag="JZ382_002365"
     CDS             337..540
                     /locus_tag="JZ382_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAY2428513.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(681..1166)
                     /gene="ssb"
                     /locus_tag="JZ382_002366"
     CDS             complement(681..1166)
                     /gene="ssb"
                     /locus_tag="JZ382_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY2428514.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1169..2068)
                     /locus_tag="JZ382_002367"
     CDS             complement(1169..2068)
                     /locus_tag="JZ382_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAY2428515.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2081..2674)
                     /locus_tag="JZ382_002368"
     CDS             complement(2081..2674)
                     /locus_tag="JZ382_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAY2428516.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2915..>4846)
                     /locus_tag="JZ382_002369"
     CDS             complement(2915..>4846)
                     /locus_tag="JZ382_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY2428517.1"
                     /translation="VVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADHGKIFKRL
                     WINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIP
                     GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQ
                     EAFEDYLKEKGVQLGKQPGTIHQVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTL
                     EAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYV
                     NNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILT
                     QVQQLQLKTVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKET
                     KPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKV
                     EKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGEQKGTQATFLANIQKFV
                     SHLLEVVPGQIQSTDFGSTLQEVKAASEKQEATRHLGVCPKCREQEVLLYQNVAACTS
                     EACDFKLWTTIAKKKLTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGF
                     EFSEKKKTNYKKRTRRTTK"
BASE COUNT         1204 a          983 c          847 g         1812 t
ORIGIN      
        1 tttatatgcc catcgatcgc gtaaaatgat cggggtttct aatttgcatc gattcaatga
       61 ctaattgatt ccgtttattg aaatgacctg tgacggcaat attgtatttt ccatcatcca
      121 taattaacaa tttatcgata atttcaatat ctgcaactac acaattaatc gattcatgaa
      181 ttgtttgtaa agtaaagcga accaataatg gttgtgtttt tattactcgg attttatcaa
      241 gcaaaccaac atatttgttc atcacgatca cctcgctaaa attatagcaa aaacagctaa
      301 aattgagaat ttttattcga ttttagatat gctataatga tcaagacagt aaagtttgtg
      361 acggtggcta tccgattgaa agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca
      421 gaaaggagaa gccttttgcc tgttgaaacg gcgctaggat taatgattag tttcggttct
      481 ttgatcgcaa caatcatttt tggaatctta gcagttgtaa acaacaatca aaaaaaataa
      541 ccgtcagctt tagccggtcg acgattattt ttcaaaaata atattaagct ggtcaccgtc
      601 ttaaacggtg ctgttagggg agttgttagc gcaactcctc ttttcgtatc cattataaca
      661 actttattga tcttttacaa tcagaatggt aaatcatcat cactaatatc gattgattgt
      721 ccgccaaatg gattttgtga agcgttcaat ccattgttgt taggcgtagc tacatcccgt
      781 tgttcaaagg tggtagggct tggactgctg gacgattgat cagcgtgcgc acggctttct
      841 gtcgctgctt tactttctaa tagttggaaa ttatcggcca caacttctgt cacatacaca
      901 cgttgtcctt gttggttatc gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa
      961 ctgccctttt tagctaatgt cgccatcgtt tctgctggtt tgcgccaaat cacacagtta
     1021 ataaaatcag cttctcgatt gccgttttga ttcgtgaaat ttcggttgac agctagggta
     1081 aaagtcgcta ctgcagtacc actcgcggta taacgtaaat ctggatcttt ggttaagcgt
     1141 cctactaaag ttacattatt gatcatcttt atctctccat ttcttgattc atttgttgtg
     1201 atcttttaaa ctgccgtttt ctttcttgat tttcaatttt gatcacactt ttcattaggt
     1261 gatcaatttc gttgactgta tctgtaaatg aaatggaatt cgcaaacgtg ttaactgagc
     1321 gatatttttc ccactgcttt tccaaatagt catttcctct gatcttatcc atttctcttt
     1381 cataataaaa ggacaacggt tttgtatccc cacgttttgt aaatgtattt cgaacacttt
     1441 tgtataactc tttcgtatcg atattttcta gtttcggaat ggtaaataag tcgtagtaat
     1501 cttttgctct cgaattatct gtgccaaagc tgaacgtagc acttagcttt tctgcaataa
     1561 tttgttctgt tggataagct ggaatctcca cttttttatc ttcaaacatt aattggtgat
     1621 agtgtgtatc tacttcagga taaatagaat ctcctgttgt cacgtccatt ttaaacggta
     1681 cacgtacttt ctctaaatta gccaccaatt taatttgaaa tccaggataa aaatcggctt
     1741 ctcttgtttc cttcattcct tgaatagaga aaacaatgcc ttcttttgtg ggttttctag
     1801 ttaaatcatt aaaaatgctc ttcaattttt cttcagttaa tttgctattt ctaaatgtcg
     1861 tatcgatatc aattgttgtc cttctatcga ttccatattt tgatcctaat aaaaagccgc
     1921 ctttcaaaat gaagtcattt ttgtactttg attcagaaat ttgttcaatt agacgatcca
     1981 tcataaaatg ccgttgcact aactgtgcat ttaatcctgt actctttgct aaatttcttg
     2041 tttttgctgc aaattgtgtc ggtgtcatct ttttcctcca tcaatataag ctttgcatgt
     2101 ggtatttcat ttctttttct acatgtagtt ttgtcatgta atctcttaat ttacttggat
     2161 ctctcaatgg gtcttccatg tactctttta atgcttctaa tttcatttca tattcaaatt
     2221 catatctcgg attccaaata tcacaaagtg tacgctcttt atcatacata gtaataggat
     2281 ttccttgaaa ggaagttccc tctacttttc ctagttcata aacttctttc ttcacgaaat
     2341 gaaattgtaa ctgctgctct tttgtaattg aacgatcaac gttatagcca tggggaaaag
     2401 ttaagtgaat gaatttcggc atattcgtag aaagctggta aatatctaaa gtcgtttcat
     2461 gagagaagat tcccctggaa aatcgttgct gaagccaaaa taaatcatct attccatgac
     2521 catcctctac ataataacca cgatcaactt tgatcaattt tccttgattc accaatcttt
     2581 gtacagtggt tgctgcgatt ccaaatttcc tggcttcctt catagataaa tttccatcgc
     2641 gaatagccaa caattctttc agtttatctt gcatttactt cccctccctt cgttacaaat
     2701 acactacatc aaatatatca tagtagttta tttgtaacaa gattaacgtc tatcgatcta
     2761 attcgatttc tttttgaatg cgctgctggt tctttaattg ttgcagtctt cttttatttg
     2821 cacgtaattt ctcttgttct aaacgaattt ttgctttttg attttcgatt tcttgttgtt
     2881 tgtgtgttgt atttcgcata ggataacccc ttcattattt tgtagtgcgg cgtgttcttt
     2941 tcttatagtt cgtttttttc ttttcagaaa attcaaagcc tgttgtaaag tcttctttta
     3001 aaacgatcgt tgcttcaaac gaacctttct gaccttttag tcctctgact gattggcttg
     3061 ttcggccgtt ttgaatgatc tctttcagtt gtgtggctgt gagtttcttt ttggcaatcg
     3121 ttgtccataa tttaaaatca caggcttcag acgtacatgc cgccacattt tgataaagca
     3181 agacttcttg ttcccgacat tttggacaaa cacctaaatg acgcgttgct tcctgttttt
     3241 cactggctgc tttcacctct tgtaacgttg atccaaaatc tgttgactgg atttgtcccg
     3301 gtacgacttc caacaagtga gagacaaatt tttggatatt agctaagaaa gtagcttgtg
     3361 ttcctttctg ctcaccaatc tttaataagt aactttccca tttggcggtc atttcggcac
     3421 ttgttagcag cggctctttt tccacggctt ggcaaagtaa aatcccttta tttgtcacca
     3481 caagcttatt tttttctacc ttgatatatt ccttttgttt caaagtttca ataatatttg
     3541 ctcgtgttgc ttctgtaccg attccttcta cttctttcaa tatggattgt gcttcttcgc
     3601 tatccacggt ttttccagct gttttcatcg ctgtgatcaa cgtaccttcg gtataaggtt
     3661 gtggtggctt cgtttctttt tcaaggactt gtagatctac cgtcaccgct tctccttttg
     3721 tcactttagg aagtagcggt tcttcttcct tttcttttct gtcgctctct ttgccaaaca
     3781 gctttttcca acctagatct actggtactt ttccgactgt ctttagctga agctgctgaa
     3841 cttgcgtcaa gatcgttgtt tcttcatagg tatacttttc cgcaaacatg gccaccgttg
     3901 tttttaacac ttgttcataa atagcctgct gcagtggact taattgttca aaagccgctg
     3961 ccgtcatgac cgtctttgta ggaatgatcg cgtaatgttc ctggactttc ttattattca
     4021 catatcgact attcggtgta tgaataggag taggaatggc ttcggccttt aaaaagtgtt
     4081 tgtattcgtc taagtgatcc aacagatacg catactcccc ttctgtaata taaggggtat
     4141 ctgttcgtgg gtagcttaga tatttccctt catacaaccc ttgcatggct tccaaagtat
     4201 ctttggcact agctttcatg agctgattca ttttagattg aaggctggat agagaaaaaa
     4261 gacgtggact tgccgcactt tttttctcgg tttcaacttg gtggatcgta cctggctgtt
     4321 ttcccaattg aacccccttt tcttttaaat aatcttcaaa tgcttcttgc gtcgcttggg
     4381 tttcattcgg atcaagcttc gcatcaaatg cgccattttg agcgattact tgggcttttc
     4441 cttcaaaata tggttctttt tggaagtttc taatcttttc ctgtaattga taaatcatgt
     4501 aaagcgttgg ggtttggact ctgcctaagg aaaaacttcc tggtatgcct ttttgctgca
     4561 agagtaaact atacagcggg ctaccgttca tgccgatcaa ccaatcggcg atctggcgtg
     4621 cttgtgcttc ttcaaatttt gggtaggtct cttctgcatc tcgtaactgc tggaatccct
     4681 gataaatggc ttctttttct aaggagttga tccaaagtct tttaaatatc tttccatgat
     4741 cggcgcctgc ttgttgaatg atcgtccaag cgatcagttc cccttcacga tcgctatctg
     4801 tcgcaataat aatggtgtcg gccgattgaa gttgctgttt caccac
//
LOCUS       DADJYG010000160         4767 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.166, whole genome shotgun sequence.
ACCESSION   DADJYG010000160 DADJYG010000000
VERSION     DADJYG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.166"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            38..1117
                     /locus_tag="JZ382_002370"
     CDS             38..1117
                     /locus_tag="JZ382_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY2428518.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            1134..2357
                     /locus_tag="JZ382_002371"
     CDS             1134..2357
                     /locus_tag="JZ382_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY2428519.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2446..3333
                     /locus_tag="JZ382_002372"
     CDS             2446..3333
                     /locus_tag="JZ382_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY2428520.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3419..3649
                     /locus_tag="JZ382_002373"
     CDS             3419..3649
                     /locus_tag="JZ382_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAY2428521.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3706..4671)
                     /locus_tag="JZ382_002374"
     CDS             complement(3706..4671)
                     /locus_tag="JZ382_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAY2428522.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1612 a          792 c          969 g         1394 t
ORIGIN      
        1 aaaatagggc ttaagtttga gtgacagttt gtaggctatg ttatactata taagtgaagg
       61 gggacgtaag gtgaaggtat tattatattt tgaaagtgaa aagatgcttg caaaatctgg
      121 aatcgggcgt gcgcttgatc atcaaaaacg tgctctgacg gaagtcggta tctcttatac
      181 actagatgaa aaagaagatt atgacatcct gcacatcaat acatacggaa tcaatagtca
      241 taatatggtc aataaagcca gaagagaagg aaaaaaagtt gtctatcatg cgcattctac
      301 agaggaagat ttccgaaatt cctttatcgg atcgaaccag ctttctccta tcgtaaaaaa
      361 atatctggta ggattgtacc aaaaagcgga ctatctgatc acgccaacac cttattcaaa
      421 acagttgttg gaaagctatg gtatccgtgt gccgattcaa gctatttcca atgggatcga
      481 cttagagaaa tatcaccctg atccaatgaa agaacaaaag ttcagagaat atttcaaact
      541 gtctccggat caaaaagtaa tcatttgtgt cggtttgttt ttcgaacgta aaggaatcat
      601 tgatttcgta gaaattgcta agaagatgcc cgaatacaca tttatttggt ttggtcatgt
      661 tcccatgtac tcgattcctc ggaacatccg gaaaattgta aaagaagatc atcctgaaaa
      721 tgtcctgttt cctggttaca ttcgtggaga gatcatagaa ggtgcgtatt caggtgcaga
      781 tctattcttt ttcccttctt atgaagagac agaagggatc gttgttttag aagctttagc
      841 aagcaaacaa aacgtacttg ttcgagatat tccagtgtat aatggctggt tagaagatag
      901 caagaactgc tatatgggga agacaaacga agaatttatc cgactgatcc aacagattac
      961 caatcaagaa ctaccgagta cgacacaagc cggttatcaa acagcaaaag agcgaagtat
     1021 caataaaatc ggtgaagaac ttaaaaatgt atacgaaagt gtactgaatg aaaaagtctc
     1081 tagcaaatta aaaaaagtaa atcaggtcaa ggactagttt ggaggcgaaa aacatgaaaa
     1141 tcggcttttt taccgatacc tactttccgc aagtcagcgg agttgctaca tcgatcaaaa
     1201 cattaaaaca agaattagaa agacatgggc atgaagtcta tatttttaca acgaccgatc
     1261 ctaatgcgga taagttggaa aaagatgtga ttcgaatgcc aagtgtacca tttatttctt
     1321 tcaaggatcg aagagttgtt gttagaggca tgtggtatgc ttatttggtt gccaaagaac
     1381 tagaactaga tcttattcat acccatacag aattcggagc agggatactt gggaagatgg
     1441 tagctaagaa gttgaaaata cctctgatcc atacttacca tacgatgtat gaagactacc
     1501 ttcattatat cggtaaagga aaagtgatca ggcaatccca tgtaaaatac ttgtctagat
     1561 tatttgcaaa tcatacgaca ggtgtagttt gtccaagtga acgtgttatt gatacattac
     1621 gcagctatgg tgtaatagca ccgcttcgcg tgattcctac tggtatcgac attgaaaaat
     1681 tcaaacgttc tgatatcact cagcaggata tcagcaaatt aagggagtca ctaggtcttc
     1741 aggaaaacca tttgatgatt ctttctttaa gcaggatttc ttatgagaaa aatatccaag
     1801 ccttgattaa tgggtttcct caggtcatct ccgcttatcc taacgccaga ttagttatcg
     1861 taggaaaagg accttatgta gaagagttag aggaattgat ctcagaactg cagttagaag
     1921 aatttgtgca gttcacagga gaagtggaca acaatgatgt agcactttat tataaagcgg
     1981 cagattattt tgtcagtgct tctacttcag aaacacaagg gcttacttat acagaagcga
     2041 tggcagccgg aacaccttgt gtcgtggaag gaaatgctta tttgaaccga ttgttcgatc
     2101 atccttcatt agggaaaact tttgaaaccg acgaagactt tgcaccagca cttattgatt
     2161 atatttccag tgaagttcct aaagatccaa aagttttaga agctaaactt tatgatattt
     2221 catctacaca tttcggcgaa gctatgatcg aattttatca agacatggtt gtttatcatg
     2281 aagaagagat gcgaagaaaa gaagaagaag actcgattga acgaatcaaa ataaaattaa
     2341 attcatttaa aagataaaat tcaaacattt tttatgaaaa aatccttgtt tctagcgtat
     2401 actagcatag aagcaaggat ttttgatttt taggaggtcg aaaaaatgaa acttggtttt
     2461 atcggaacag gcgtcatggg aagtgctgtc gcacgccatc tactagaagc aggacacgaa
     2521 gtagctgtct ataatcggac aaaagcaaaa gctgatcctt tagtaacaga aggagctatc
     2581 tgggcagata caccaaaagc ggtagcagaa caaagtaata ttctctttac aatggtaggt
     2641 taccccaaag atgtagaaga aatctactat ggacagtcgg ggattttttc tgcagatatc
     2701 tcaggacata ttttagttga tttgactaca agtacaccaa gcctggcaga aaaaattgca
     2761 aaaacagcaa aagaaaaagg cgcagatgcg ttagatgcac cggtatccgg tggtgactta
     2821 ggagcaaaga atggtacact gacgatcatg gtcggtggag aagaagccgt ttatgatcaa
     2881 gtactgcctt tattcaaaga atttggtacg acatttacat tgcatggttc tgctgggaaa
     2941 ggtcagcata caaaaatggc taatcagatc atgattgcag gtacgatgac aggaatgacg
     3001 gaaatgcttg tctatgcaca aaaaaacgga cttgatttaa agaaagtgat tgagacgctt
     3061 tcaggtggaa gtgcagctaa ctggtcttta agcaattatt ctcctcgtat tttaaaagaa
     3121 gattacacac ctggtttttt tgtcaaacat tttatcaaag atttaaagat tgcattagaa
     3181 gaagcagaaa aaatggatct tgtattgcca gcaactaccc aagcattgaa actatatgaa
     3241 gagttggcag ataaaggttt tgagaatgac ggaacccaag ctttgatcaa actttggtgg
     3301 cctgaaggaa aaataccaga aaaaaaatca taaaaaacga gtttttttag agaaaatcaa
     3361 tgcaagtagg ctatgctttt gatacaatat aacagaaata agaaaaggag gacctccgat
     3421 gacacattca caattagtcg caatcataaa acgactggag gctatggtcg agtcagcaga
     3481 taatgaactg caagttcgcc gttttgaaaa agaaggcgta gagaagtgta cagtaactta
     3541 tgataaagcg accgatacat ttgaattaac tgaaacagat actcatcagc aatacgaatt
     3601 tgataatatc gatatcgtag cgatggaaat ctatgactta atccaataaa ttttaggata
     3661 attgacattt gaagttgaag atgcctaaaa gaagccgaga agcaactaat ttagttgctt
     3721 ttcagcttct tttttgttta acagttcaat ttcaaactgt tctttttcta attttttgcg
     3781 gtaactgatc aagatttgta caggatcgat ttcttctttt gacaattgac gtttcaaaga
     3841 taaatgagct tctttctttt cataatagaa gtcaggagaa acgtcaccag aggtaatcat
     3901 tgtttctgca agttgaccat aacaaacgag gtagtgcttt ttctgactct catctgctgc
     3961 taatggcatg ctgaaggtga gcgatggtgt tcttaaaaac tgcttttgtt cctggatccg
     4021 tctaataaaa taagtaacta attcgatatc tttttgaagc ataccggctt tttcgaaatt
     4081 caactgatag atgaaatgtt gttgtctttt tttcaagtaa gtcagtagct ttttatttcg
     4141 gccttgaaac agcccttgga gttccttttt tttctgttca aaagtcattt cctgtaggtc
     4201 aggaagctgg actcgcaaag ctaacaaact aatttcagac aaccaaggca tttgataagt
     4261 ttcttccatt atacgcaaaa tagacggcat ttttttatag agacgaaaag gcccgtatgt
     4321 tcgagccgta ggagtatctg ttaatacaaa tcccttattg gtaatatgta cataattata
     4381 attactgaaa taattcattt gtcggttata aaaaggacgg taatgttgga tcaattgaca
     4441 ctcgagcaat aaagcatcta attccgtgtc tactacgacg aaatcaaaat ctgttatatg
     4501 atgaatcata cgtaatactt tttttgaatg ctgtttattc tgatgaaaat agctgcttac
     4561 tctatttttg agccgttttg ctttaccaac atagatgacc ttaccatctc ggtctttcat
     4621 aagatagaca cccggcagca tggggagagt tttcattttt tcttttaaca taggatctcc
     4681 ttttacgctt tgaataaaaa gactataaca aattttgaaa taatggacaa gttttaggga
     4741 attctatagt tctgttcaca gaaaata
//
LOCUS       DADJYG010000161         4754 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.167, whole genome shotgun sequence.
ACCESSION   DADJYG010000161 DADJYG010000000
VERSION     DADJYG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.167"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            149..400
                     /locus_tag="JZ382_002375"
                     /pseudo
     CDS             149..400
                     /locus_tag="JZ382_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            493..1287
                     /locus_tag="JZ382_002376"
                     /note="aph(3')-IIIa"
     CDS             493..1287
                     /locus_tag="JZ382_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAY2428523.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1697..1808
                     /locus_tag="JZ382_002377"
                     /pseudo
     CDS             1697..1808
                     /locus_tag="JZ382_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1829..1912
                     /locus_tag="JZ382_002378"
     CDS             1829..1912
                     /locus_tag="JZ382_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAY2428524.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            2037..2774
                     /gene="erm(B)"
                     /locus_tag="JZ382_002379"
     CDS             2037..2774
                     /gene="erm(B)"
                     /locus_tag="JZ382_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY2428525.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(3037..3900)
                     /locus_tag="JZ382_002380"
     CDS             complement(3037..3900)
                     /locus_tag="JZ382_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAY2428526.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3902..4174)
                     /locus_tag="JZ382_002381"
     CDS             complement(3902..4174)
                     /locus_tag="JZ382_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY2428527.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4191..4406)
                     /locus_tag="JZ382_002382"
     CDS             complement(4191..4406)
                     /locus_tag="JZ382_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAY2428528.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4498..>4754)
                     /locus_tag="JZ382_002383"
     CDS             complement(4498..>4754)
                     /locus_tag="JZ382_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAY2428529.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1461 a          806 c          870 g         1617 t
ORIGIN      
        1 gaatcaattc tgattcatca gtcccataaa ttatagagcc catttcttgg gagttttgag
       61 actactatat gcgttttttt gttgatttac caacatgttt agacattttc ctctaggaaa
      121 aaattaacta gcaccacggg ttatctttta tatagaagat atcgccgtat gtaaggattt
      181 cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa
      241 aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta
      301 tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
      361 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
      421 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga
      481 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
      541 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
      601 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
      661 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
      721 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
      781 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
      841 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
      901 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
      961 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
     1021 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
     1081 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
     1141 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
     1201 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
     1261 tatattttac tggatgaatt gttttagtac ctagatttag atgtctaaaa agctttaact
     1321 acaagctttt tagacatcta atcttttctg aagtacatcc gcaactgtcc atactctgat
     1381 gttttatatc ttttctaaaa gttcgctaga taggggtccc gagcgcctac gaggaatttg
     1441 tatcgataag aaatagattt aaaaatttcg ctgttatttt gtacatttaa cttgacggtg
     1501 acatctctct attgtgagtt attagtggta cagttttcaa ccgttttaat tataaaaaag
     1561 tggtgcattt ttaaattggc acaaacaggt aacggttatt gcaggtgtat ttcttatcta
     1621 tgggtttaac atggatttta tcattaaaat catgagtatt gtccgagagt gattggtctt
     1681 gcgtatggtt aaccctaaag ttatggaaat aagacttaga agcaaactta agagtgtgtt
     1741 gatagtgcat tatcttaaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
     1801 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atgtagataa
     1861 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
     1921 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
     1981 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt gataacatga
     2041 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
     2101 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
     2161 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
     2221 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
     2281 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggaatattc
     2341 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccgt gcgtctgaca
     2401 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
     2461 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
     2521 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
     2581 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
     2641 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     2701 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta
     2761 acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa
     2821 gggaatgtag ataaattatt aggtataggg cacctctaat aactaccaat taattcacct
     2881 caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct atttcttatc gatacaaatt
     2941 cctcgtaggc gctcgggacc ccaacatgag gaaatgtttt tataaaaaaa gagtagttac
     3001 cgttttttgg taactactct tttccttttt taaggtttaa atacctggaa gtttaggtgt
     3061 tttgggtatt ggtggtgtag gtggctgcaa gctttccaat ttttgttgaa ttgctttaaa
     3121 ttcaggtgtc tcttggtgtt tatttagaac cattttttgc tctattcgtt ctaaagtcgg
     3181 ttgaatttct ttccctgata ctttacgatt caattctttt tctaaggttt cttttggact
     3241 aatagaaggt gtttccaaac ttgaatagag ttttactcct tctctgttat atagccttat
     3301 atcgctaaat aagcccgttt tatgaagggt ttctaaattg gtcggtaagt ttttgacaac
     3361 aatatcatgc gcttgttttg gtgttgccct ggctgtcatt ggatcatctg catacatggt
     3421 ttcatatcgt tcaattgttc ctaaatatga gttgatttta ggtactgcca tgacatacat
     3481 ttttgtttca taacctttgg cttgaagcat tgttgcggtt tgaataggaa cgtctgttgt
     3541 tcgtcctgta ccttcgatca ccaaattata cccttgatcg ctcaaacggc ttatgatcgc
     3601 ttctgtcatg cgattagaat aaggggtaac gtgttttact acgtcttttt cataaagttt
     3661 cactagttca tcaaaattag ggtgctgttg tttaaaggta tcattatcaa tgacaataac
     3721 attcccttgt gtttcttcaa aaattgctga tcgcaaactg gttttccctg accctggttg
     3781 cccaccaagt aaaaaagcgg ttggcgattc aaccgctttt tttccttgaa tcaattcttc
     3841 taaattatca tttaagcgat tctcaaattg tttgtcagta aaattgacta tatttgccat
     3901 attaagccac tttctcttta ttcaaaactt gttttgagta acgattcatt gaccgccaat
     3961 actcatgaac ggcttgtaat tcttctaaag aataatcaaa aagatttaca cgttttgcaa
     4021 gctttaattg gtttaataaa ttgacttcca ataattgaga gtcattttta tttaattcat
     4081 ggttcaaaac atagtttaat actcgattat aaacgcttgc cgataattcg ttaatgatct
     4141 ctatttcaaa tgttttttca tacgtaactg ccatttcatt ttcactcctt ttaaagtttg
     4201 tcaggtaaat atttccgtat atattcttct aaggcttgat ccataacttc ttttacgttt
     4261 ccgccattct ttgctgtttc gatttttatg atatggtgca agtcagcacg aacacgaacc
     4321 gtcttatctc ccattatatc tttttttgca ctgattggtg tatcatttcg ttttgctttt
     4381 tgtgcgccta aatttcccac aatcactcac ttctttctat ttcttcttac tcttatttta
     4441 tcatcaacaa tcacaaatca caagtgattt gtgattaatc acttgtgatt tgtgattcta
     4501 ttctttttcg ttttctaatt gaataattcg ctcaagcatt tcaaaaaata cgttcttata
     4561 catggataaa acttttttgt catagccttt atgctctgta atgccgtttt tagaccaggt
     4621 acttacttta ttacttcgct tgataatatt ttggaaaacc aagttatctt ctttatgttg
     4681 cttatacagt tcttccaggt ttgattttat cgttgcgctg tccgtatcaa ctaaataagg
     4741 aacaaaaccg atca
//
LOCUS       DADJYG010000162         4642 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.168, whole genome shotgun sequence.
ACCESSION   DADJYG010000162 DADJYG010000000
VERSION     DADJYG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.168"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..945)
                     /locus_tag="JZ382_002384"
     CDS             complement(<1..945)
                     /locus_tag="JZ382_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2428530.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISIL"
     gene            complement(947..2386)
                     /locus_tag="JZ382_002385"
     CDS             complement(947..2386)
                     /locus_tag="JZ382_002385"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAY2428531.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2571..4517
                     /locus_tag="JZ382_002386"
     CDS             2571..4517
                     /locus_tag="JZ382_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAY2428532.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1478 a          788 c          764 g         1612 t
ORIGIN      
        1 aagtattgag attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag
       61 aagaatatca acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg
      121 tgggaaaaat ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg
      181 gatattaagt ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga
      241 aacaaaaata ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg
      301 gatagtagac agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa
      361 acctagtgca cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt
      421 attttgacaa ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc
      481 tgaagaaaca atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct
      541 cacacgctca taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg
      601 gttggcttca agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca
      661 aataatcgtt ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga
      721 gtaaaagata ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg
      781 aagtccacgt gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt
      841 ttgttttgtt ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg
      901 gctaactcca gctaattcag cgacatcagt taattttgct accattttaa tattttaatt
      961 caaagtattt tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag
     1021 ggaatacacg actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt
     1081 tatctacaaa aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac
     1141 caaagtcagt ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt
     1201 tggtatctac aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg
     1261 atttctgatt tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg
     1321 ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa
     1381 gtttaccatc ttttattgaa agttctttta caagactcat tgctccctga taatcatact
     1441 tatcagttgg ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat
     1501 caggagtatt gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt
     1561 ctgaagcgcc cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat
     1621 tcggatagat attcccataa tttagctcag atttatccaa cccttgagga caataaatga
     1681 gaacaggttt tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag
     1741 tgccgccaaa atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt
     1801 tgtaaagttt gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt
     1861 gtccttcata attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat
     1921 gaataagaac attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt
     1981 gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat
     2041 tcccctctat ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg
     2101 gaagaagttc tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc
     2161 attctttcaa cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc
     2221 cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat
     2281 agctagcacg ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag
     2341 tccaatcaga ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca
     2401 atttatcttt ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt
     2461 taaaaaaata aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt
     2521 tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca
     2581 gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac
     2641 actgcgctac acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt
     2701 tagacgaaaa tccagatgtg aaaagcacat ttaaaattaa tggacaatat caagtgatta
     2761 tcggtgcagg tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg
     2821 aaatttcaac agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta
     2881 tggcattaat taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag
     2941 gtggtttatt aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat
     3001 caattgaaga aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg
     3061 ctgcaccatt catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag
     3121 caaaccaatt tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct
     3181 caactgaata ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag
     3241 ttattccagt tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa
     3301 aattgccatc atctattgac tttacattca cgccattatt atcagtaatg attactggat
     3361 ttttgacttt cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca
     3421 ttattgtttg gctatataac actactggat ttattggaat gggtattttt ggaggaacat
     3481 attcacttat agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac
     3541 tttcagcgtg gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg
     3601 caaacgttgc tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga
     3661 caaaaagtct tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct
     3721 tgttcggagt taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa
     3781 ttgctacagc agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt
     3841 tcttgggatt tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga
     3901 ttagctttgt agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta
     3961 tctttaaagc agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa
     4021 cagatccaat atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg
     4081 ttagtcccat ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat
     4141 cagaagctat gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag
     4201 tagatggtac tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata
     4261 atggtgctga agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat
     4321 ttacacaaaa agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg
     4381 accgttcaat gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata
     4441 cttcggatta ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt
     4501 tacttgaaat caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca
     4561 aataatatag attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca
     4621 attgtatgaa aaattattag aa
//
LOCUS       DADJYG010000163         4622 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.169, whole genome shotgun sequence.
ACCESSION   DADJYG010000163 DADJYG010000000
VERSION     DADJYG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.169"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(585..1397)
                     /locus_tag="JZ382_002387"
     CDS             complement(585..1397)
                     /locus_tag="JZ382_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428533.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            1560..1667
                     /locus_tag="JZ382_002388"
     CDS             1560..1667
                     /locus_tag="JZ382_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428534.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            1725..2792
                     /locus_tag="JZ382_002389"
     CDS             1725..2792
                     /locus_tag="JZ382_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAY2428535.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            2934..3137
                     /locus_tag="JZ382_002390"
     CDS             2934..3137
                     /locus_tag="JZ382_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAY2428536.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            3197..4399
                     /locus_tag="JZ382_002391"
     CDS             3197..4399
                     /locus_tag="JZ382_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428537.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
BASE COUNT         1542 a          735 c          967 g         1378 t
ORIGIN      
        1 gaagtaatta tgtatatttt tgaaacaaaa atttttcatt gcaataattg caacaaagaa
       61 atttcatcgg gggaagaggt ttggacaaaa atggaaattt ccgccaaaag taactgctcc
      121 tcaactgaaa tcaattaaag aactagaatt tgagaatgca ctggtaattt gtatgacatg
      181 tactgatgaa gtatttcgaa aacattttta aaggttgaga tgtatataga tttaaccaat
      241 catttatctc tctcggaaaa ggaatattaa ctaaccagcg ggttatcaaa aataggtagg
      301 aaaactgtac ttaatggctt atttcgcgct gattatgaga atatcagtct gtttgattaa
      361 tttttagggt tcaaaataca gagacaaact ccaggtcggg tttcgccaca agcgaattac
      421 ccgatctgga gttttgtcta ttgcaggaag gcgtagccgg actgcaggaa taaagccccc
      481 ggttctaaca gggggctaac aaagaacagg aaactgtgac aactacccat tggggagtgt
      541 cctagcagta ttttctttgt cacgtgctta tcaattttgt cacctcattt tgatgatgga
      601 tacgctgcat ttaacaggcg ttctttctcg acttccatca cgaatggtag ataaagatat
      661 tgctgttcta gtagatattg attgtcctct gtgtactgat aagaagcggt ttcttctgaa
      721 tagaccaacg gggcaattcg cacatcgctg gtcaattgat taattgagta tagaaggatg
      781 gctttctcac ctagttcctt gggtaaatgt ttcccaaaat aggaatagga tttttgattt
      841 gctaaataga cgagtatgcg atccacacta aagttggaga aggtaaaggg catcatgtgc
      901 agaatatgat tttgattatc atgttgcaaa tagagctggg cttccttacc aaaattagtt
      961 gaaccaagat aaagctgctt tttttcatcg atacattgga aagtaaactg gtcttgttca
     1021 gttggtggaa tgagatttgt aataaaaaat tcaatcattc gatccatttc tttttcacta
     1081 acaaggtcag cgtgtttgat ttcgtctact atgagacctt caatcttagg atcaagctta
     1141 cctgcctttt tttggagcat ttctaaaatg tattgattct cttgatcacc atacaaaaga
     1201 tattcattgg tgacatgacc tatctctgcg atttggtgaa gggtttcggt ttttggaagg
     1261 ttgatcccac gttcccagtt attgactgta cttcgcggta aatcaccaat tagtttgcca
     1321 aatttctcca tactcaactt taattcttgg cgaatctgcc gaattcgatt gccgactgct
     1381 tgcttgtcta tctccatgaa aactcctcct caggataagt tcttaaattt agtatacgtg
     1441 tgtacttaaa aaaagtcaaa acatattgac ttaaaataaa tacaggcgta caataaaaat
     1501 gtacttaaaa taagttcaat aggttctttg acaagtaaat agcagaagga ggtttgtcga
     1561 tgaaccaagt taatcaacat gatctcggtg aagccattcg agtttctcga gaagaaaggg
     1621 ggtggacaca acgatattta gcagaaaaag tggggatttc gagatagtta ttatcgaaag
     1681 tagaaaaagg aacgagacaa ttaagtgaag aaaaattgaa tcttgtgttg gatagtttgc
     1741 aagaagaggt cgttccagtt aaccgtgttt tgattgatta tctaaccatt cactttttct
     1801 cgaatcaaca tttaaagcta attgaagaga ttattgaaat gcccattgaa cgttttgagg
     1861 aactggatta tgcgccaaag ggctatattg gccaatacgt gtggaatcaa gtaatcacga
     1921 ttcggtattc cattgacgat gcggttaaag gaactgtgat ggagttttca ggtcaaggct
     1981 gtaaacactt agcaagatgg ctaaaaacac gaaaaacaaa ttggcaacag ttttttcgta
     2041 aggtattaga ttatcagggg aactttacac ggattgattt tactttagat gattttgtgg
     2101 ggagtctcag cattccagaa cttaagcata aggtaatact aggccatgtg tggaccactt
     2161 ttcaagtgag tgaatctcat ggcggtacgg atatcataaa caatgaatca aatggtgaga
     2221 ccttgtattt aggctcaaag aagagtcagt gtcgtttttg cttttatcag aaggaccatg
     2281 aacaacggaa gcgacgagga attcctttag aagaggcaaa agtaaagaat cgctttgaac
     2341 tacggtatcg aaaagagaaa gcgcaaagct tggcaaagat tatttcaaaa acccatgatt
     2401 taaccaaact gttctttgag ttacttaatg gagcaatttg tttttatgac cgtgatccaa
     2461 atgatctagg tgcaaaagtt gatgccaaat gggcagcctt tattagcaat tatggcgcaa
     2521 tcaccatttc tttagataca attcctcaaa gttttgagaa aactatgagt tggttaattc
     2581 atggtgtttc accatcatta gtttttattg cacaggtcga tcaaacgttt cattcttcct
     2641 tgttgccttt gattattgaa caaggagaaa tgaatccaag acagatcaaa cttctccaaa
     2701 atatgaaggc agacccggag tattatcagg aggaggttga gtattatgaa cgaaaattac
     2761 aaagtatatc aaatgagaaa acaagctact aaaaaagcaa agtactgctt acacaacaag
     2821 ttgtgcaacg tactccgcct cgacaagacg agtataacac aaccatcgaa tgagaaacaa
     2881 tagattataa agaggattat tttaaaagga gtaaagaatc aggaggtgag agaatggaaa
     2941 caaaatgtga tttcatggta aatcgtgcaa ttttgataga gatgggcttt aaacctagtc
     3001 aagctgcacg aatgattaaa gagagcaagg catatcttgc aaggattgaa ggtattgatt
     3061 tttataataa tcgccaagtt ggtgtggtgc catcccgagt aattgaacat ctttttcaca
     3121 ttcaagttgc ggaataaaca gtacaagcgc ggtactatta aagtagcttt tctcaagtac
     3181 agggaggaat ggaacgatgc caaaaattaa aaatatttac caagataaaa agacaaaaaa
     3241 atggttcttc cgagcgtatc taggtataga tgaaaatgga agaaaggttc agaagactaa
     3301 acgaggttat gcttcacaaa aagatgccaa aattgcttat gacaagtaca tggaaacaca
     3361 tgattttaac aagacaattc ctaatcaact gtgttctacc aatcaaatga ctttcgaaga
     3421 gttttacaag gataggtttg taaaatggta cgagagacag gtcaaaagtc aaacatatga
     3481 gaatgcgcaa ttcattttcg aaaagcgtat gcagtttttc tatcattttc gtgttcgaga
     3541 tattacaagt cacgatattg aggattggtt atttgaacta agccagacag agtcacgtaa
     3601 ttcacggaaa aaagttaatg gcgaaacgtt atcaaaatct tatatcaatc ggattcgagg
     3661 gcacctaaaa attgtattgg atcgggctgt taaagagggg gtgattgcta aaaatcccgt
     3721 tgaagatgtt tcttcgcttc cttctgagaa taagaaagtt gagttttggg aacacagtga
     3781 gtttcaaaaa gtgatgaatg aattgaagga tgataagatt caaacccacc atcggaagat
     3841 tgtgtatgaa atgctctttt atactggcat tcggattgga gaacttgaag ctttgtcttg
     3901 gtcgaatgta gattttgaga aaaacacgat aacgattgag aagacattga tttataatac
     3961 aaaagatgat tggtactttt cgactcccaa aactaaatgt gcttatcgaa cgattgggtt
     4021 ggggaagaag ctttccttaa aactcagaca gtggtatgac ttgcaaaaac gtattggaaa
     4081 ttttgaatat gttgctcagt tggacggaac atttacgcca ccttattctt ttgcgaactg
     4141 gttaaaagaa gctgctaaaa aagcgggtgt caaaccgatt aagcttcacg cgctacgaca
     4201 ttctcatgtt gcatttctaa ttgagcagaa tattcaaccg ctatctattc aagaacgatt
     4261 gggccatgcg aatattcaaa tcactttagg aacttacggt cacttatatg caaagtcaga
     4321 tcaacaagtg gtaagtgcga ttgataatgt tcaagagggc gctattcttt caaaaaatga
     4381 caatttatta ccagtctgag ttttccacgg ttctaccata agacaaataa atgcttttgc
     4441 accagtgttt tcagtcgaat tgctcctgtt caagatgtat cggaaatagc gagaaaataa
     4501 gcaaatttgc ataactataa taaaaaggac ttagttttga tgggaaattt ttcaaaatta
     4561 agtccttttt tatgtggttg aaacgggtat gatcatttta aggaagtgat ctaagatgaa
     4621 ag
//
LOCUS       DADJYG010000164         4611 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.170, whole genome shotgun sequence.
ACCESSION   DADJYG010000164 DADJYG010000000
VERSION     DADJYG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.170"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(86..310)
                     /locus_tag="JZ382_002392"
     CDS             complement(86..310)
                     /locus_tag="JZ382_002392"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY2428538.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQL
                     NLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="JZ382_002393"
     CDS             complement(451..705)
                     /locus_tag="JZ382_002393"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2428539.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="JZ382_002394"
     CDS             complement(898..1173)
                     /locus_tag="JZ382_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAY2428540.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="JZ382_002395"
     CDS             complement(1145..2098)
                     /locus_tag="JZ382_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY2428541.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="JZ382_002396"
     CDS             2710..4203
                     /locus_tag="JZ382_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAY2428542.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="JZ382_002397"
     CDS             4338..>4611
                     /locus_tag="JZ382_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAY2428543.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DADJYG010000165         4500 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.171, whole genome shotgun sequence.
ACCESSION   DADJYG010000165 DADJYG010000000
VERSION     DADJYG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.171"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(48..659)
                     /locus_tag="JZ382_002398"
     CDS             complement(48..659)
                     /locus_tag="JZ382_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY2428544.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(782..907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(989..1705)
                     /locus_tag="JZ382_002399"
     CDS             complement(989..1705)
                     /locus_tag="JZ382_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAY2428545.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="JZ382_002400"
     CDS             complement(1711..2280)
                     /gene="scpB"
                     /locus_tag="JZ382_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAY2428546.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2264..3061)
                     /locus_tag="JZ382_002401"
     CDS             complement(2264..3061)
                     /locus_tag="JZ382_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAY2428547.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3031..3402)
                     /locus_tag="JZ382_002402"
     CDS             complement(3031..3402)
                     /locus_tag="JZ382_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY2428548.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="JZ382_002403"
     CDS             complement(3421..4308)
                     /gene="xerD"
                     /locus_tag="JZ382_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY2428549.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1223 a          981 c          786 g         1510 t
ORIGIN      
        1 ttttttccaa aaaaaaagac caagaaattt cttgatcttt ttgatattta tttgactgct
       61 gtatgacgtt gttcgaactg atgttgtttt cgacttaacc atggaagaag ttgcgcatgc
      121 agcactaaaa agacagcact cacgatgaat cctttgatca agttgaaagg aacgatccca
      181 atcagtacat agtttgctaa agacatgccc agcatctgtg ttgcgttcag ccctaaaaac
      241 attaagtaaa gaggcgtaat aacgaaataa tttgcgatac tcataaaaat agttagagcg
      301 atggttcctg taatgacacc taaaaatttg tttcttcctt tatgctgttt ctttacacta
      361 aagaaataat aaatcgggaa tgtaaagatc gttgttgata agaaactagc agtgtctcct
      421 accaaattat ccggagaaaa acctgtagtc agcagatgta atactgaacg caagaacgct
      481 gttgcaactc cggccgctgg accgaataag aacatgctga tcaataccga gatatcacta
      541 aaatcgatct tcaaaaaact aaaagccggc atgatcggaa aagcaaaata ctgtaaaact
      601 aatgcgatcg cagcaaacat cgctactgca accattttct gtacacgggt gttcttcatt
      661 tgaaatcctc ctgttaggac tcctcttcag cgaacttttt gacgtttctc tccaaatgaa
      721 aaaactctat tttccaggga aaatagagtt agagttattt gatcaagcgt cgtcaaataa
      781 gctctatctt ctttatccag actatactgt cggtatcgga atttcaccga ttcagctatt
      841 tttttgcaaa ataggtcgtg gactataacc accggtcggg aatttcaccc tgcccctgaa
      901 gacgaacctt atataatttt tgtttacata ttcattatac atatctattc aaaaaacgca
      961 acatctaaca attataaaaa gtaagtgttc attttgagtg gttcagcaaa ctgctgactt
     1021 cttttttgtt cagttcacgg taatccccag gacgtaggcc ttgtaaagtc agatctccgt
     1081 atttttcacg ttttagtttt tgcacaggaa gaccaactgc ttgaagcata tttttcactt
     1141 gatgattacg tccttcgtgg atcgtcaatt ccacgatact gtggtttgtc ttaggatcca
     1201 ctgataaaat ttggaattta gctggagatg tttttcgtcc ttcgatcttt attccttttg
     1261 tcaaaggtgc aagcatcgat tttgtagcga tcccttttac ctttgcaacg taaactttat
     1321 ccacttcatg ttttggatgt gtcaggcgct gagcaaaatc tccatcattt gtcagtagca
     1381 agatccccga agtatcgtaa tccagtcgac ctacaggata gatacgttct tttaccatcg
     1441 gcaaaagatc cgtaacaact tttcttcctt tgtcatctgc aacagcagaa ataactcctt
     1501 taggtttgta caataaatag taaccgtatt cttcttgata aattgggact tcatccactt
     1561 ccacagtgtc atgtttccca acttttatcc ctaattccct tacaacttgt ccgtttactt
     1621 ttacacgtcc tgcaaggatg aattcttctg cttttcgacg agaagtgatg ccagcgtggg
     1681 caattacttt ctgcaatctc tccattacaa ttattctcct ttattcattc catcaaagaa
     1741 tagatctggt gacacgtctt cttcaagtga aatttccatt tcttgtgtat caggcagttc
     1801 ttctaatgat ttcaaaccaa aataatctaa gaagtagcca gttgttccat ataatatcgg
     1861 ccgacctggt ccttctactc tacctttttc ttcaatcagt ttatgggcag ctagacgctg
     1921 taccgctcct gaaacctgaa ctcctcgaat ctgctcgatt tcagctcttg tgattggctg
     1981 tttataagcg atgattgcta aggtttcgac ggcggcttga gataatgtgt ttgatgtccc
     2041 ttgcgcatat cgcttaagta agggagccag ctctttttta gtcgttaata cataatggct
     2101 ttccgtttca aaaatatgca aagctgagtg cgagttttga gtgtaagatt cttttaattc
     2161 tttcactaaa cttgtgactt ctgcttcgct gagatttaac agtccgctta attcttcatt
     2221 actgatccct tcctcgccaa caataaaaag aagggcttca agttcactta atttcgtcat
     2281 gttcggtatc tcctactgct ggataaagca taattgattc atagttttct ttttgttgcg
     2341 caatgatctg cccttttttc atcaattcta gcaaagccat aaaagtagtg acaacttcac
     2401 ttttagaagg tgactcaaaa aaagaatcga aagtacagcc atgcgcattt ctttttatac
     2461 gctcttcgat aaataccatt cgttcttcga tcgttgtgtc atcgggcgtg atcgtttttt
     2521 ccactttttg agaaaatttc ttacgttcta agattttctg catggcacgg atcaaatcga
     2581 tttttttgac ttgtcctcgc ttcaacggct tttcagcatc cgctaaccca tcgatgttca
     2641 taggttcttt tgtgtaataa agacttcgtt cctctgcttt ttctgtcagt ctttcagctg
     2701 catatttata tttacggtat tctaaaagct gagtgaccaa ttcttctcga ggatcttctt
     2761 cccataattc ttcatcgtct gtgccgaatt cttggacagg aagcaatgtc ttgcttttga
     2821 ttgccatcaa tgtagcagcc atcactaagt attctcctgc aacagctagc tcgagtgttt
     2881 tcattgtatg gatataattc atatattgct ccgtgatcgt agcgatcggt atatcatata
     2941 tatcgatttc catttgctga atcagatgaa gcagcagatc gagaggaccc tcgaatacat
     3001 ctaatttaat attgatctcc gtcaactcat tcactctttc ttctttttct ttgctagcca
     3061 ttttccaatg taaggctctg tgatttcagt tgcacggatg gagacatccg ggtacttttc
     3121 ctgtaattca tctaacattt gaaagcccat attctcaccg cgataagacg gattcaaaga
     3181 aatgtcatgg atgatcagct gatcgttcgt tgcccagtaa ataccaataa tcccttgaat
     3241 gttggttgaa ccttctggcg taaagaaaaa aagttcataa tcgtcttcag attcgtacgt
     3301 atcaatctcc ttcaacaaag aagttttacc tgtcagtttc ttatggaaac tcaatagtcc
     3361 catagcaatt ttacggtttt ctttccggta atgtgttaac atcttctcac cttcctatga
     3421 ctaagccctt ggaaaatgct gcttataaac atccgtcatt cttttcttcg tgatatgtgt
     3481 ataaatctga gtcgtagaga tatccgcgtg acctaacaat tcctgtactg tccgcaaatc
     3541 agctccattt tctagcagat gtgtggcaaa gctatgccgc agcgtatgtg gtgttacttc
     3601 tttgtaaatc ccagcctctc gtactaactg cttcagattt ttccagattc cttgccgaga
     3661 aagacctttc ccatgattat tgacaaatac atgcatttca gatgcgtcct ttacaagtaa
     3721 tggacgtgct tcgtctaagt agcgttccag ccattggatc gcataatcac ctaaaggaat
     3781 gatccgctct ttatctcctt ttccaactgt ctgtaccagt ccaagagaaa gatgaagatc
     3841 tcccaatttc aatccaatca attcactgac gcgcatccca gtagcataca tgacttccaa
     3901 gattgcacga tctcttatcc ctaacgtttt tgtcgtatca ggtgcttcaa tcagcttttc
     3961 tacttctgtc agagtcaatg tacttggtaa tttctgtgct ttcttcggcg tatcgatatg
     4021 ttgcatcgga tcatgatcag tgatacgctc ttgccgtaaa aactgatgga atcttctgag
     4081 acttgagatc atccgagtaa cagttgctgt cgcttttttt tcttcatgca acttctccag
     4141 aaaacggatg actaaaaagc gatcgacatc ctgccatgaa gcaattttct gctcctgtag
     4201 aaaatgcaag tactgttcca aatcacgttc atagctttgt cgtgtattgg cggacaatcc
     4261 ccgttcaata ttcaagtagt ggagataatc gttgatttgc tctttcataa taatccccct
     4321 catgcctctc tatcatatca gataccgctt cattctgata gtctttttcc ataatctgag
     4381 caaagaaaaa gccgtctaga cactcagacg gactggtttc ttttttcggc tctataattt
     4441 atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg tattaaaaaa
//
LOCUS       DADJYG010000166         4487 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.172, whole genome shotgun sequence.
ACCESSION   DADJYG010000166 DADJYG010000000
VERSION     DADJYG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.172"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..940)
                     /locus_tag="JZ382_002404"
     CDS             complement(<1..940)
                     /locus_tag="JZ382_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HAY2428550.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLL"
     gene            complement(937..1395)
                     /locus_tag="JZ382_002405"
     CDS             complement(937..1395)
                     /locus_tag="JZ382_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY2428551.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1392..2855)
                     /locus_tag="JZ382_002406"
     CDS             complement(1392..2855)
                     /locus_tag="JZ382_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428552.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2837..3769)
                     /gene="pfkB"
                     /locus_tag="JZ382_002407"
     CDS             complement(2837..3769)
                     /gene="pfkB"
                     /locus_tag="JZ382_002407"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY2428553.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3911..>4487
                     /locus_tag="JZ382_002408"
     CDS             3911..>4487
                     /locus_tag="JZ382_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2428554.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1344 a         1012 c          719 g         1412 t
ORIGIN      
        1 ctaatagtgt acttaattct ttaatatttt gttttccttc ttctaacatc caactactcg
       61 ctttttcttc gccttcggta acaaaaggtg ctactccctt cgcccatgtt gctcgcccac
      121 ataagacacc attgaatgtc gaacctgatt gttttgcaaa acgcaatgta tcttgaaata
      181 gtgcagcgct gactcccgca cttaaaaaaa taaacggttg ttcagtcact ttcgattgtt
      241 catagaaata cgcggatgcc tctgcttgac tataacaagt atcttgccca tatccttcca
      301 caaatctcat attcactggt acttctactt tcaatacatc cacttgataa cgttcttttg
      361 aaaattctcg catggcttca atcactttat gcggttttac ttttgcatag tcaagagaag
      421 acggatcgcc aatagttgca tcgtaagaaa ggagctctaa aaagaaaggt atgccttctg
      481 cttcgcattc tgaaccaatc cgttctataa ctatttgttt ttgcacatta atctctactg
      541 gttcatcaac gtcatagtac aataaaaatt tacaagcatc cgcacccact tcttttaacc
      601 ttttcactga ccaactattc agtaaatctg gtagtcggcc tggcattttc gcatcatagc
      661 cggttttttc ataagctagc aacaaaccac attctttctc tcgaagcgct gctgcaggca
      721 gaccatattc tggatcgagt aaaatagcgg aagcatacgg agtcaggtac tgagaaacca
      781 tctgtttaaa ttggatgata tcttctgatg tagcttcttt atattggttg atcattttct
      841 tcaaagcacc acgttggtcg atagctagcg cgctaataat tccagcttca tccgtcaatc
      901 gcttcaaacg ttcctgtttt tctatactca ttttcttcat atatcttcac ctatttcttt
      961 agttaatatt gtagctattt ctatttttga ttttgcagct ctcaactgtt ctgtaacctc
     1021 ttttcgcatc aatgatcgtg caacgagaga caataatttc aaatgagtcg ttcctgcttc
     1081 tgaatctggg ataaataacg caaaaataaa atcaactgac tgtctatcta aactgttcca
     1141 ttcaattcct tttttcagtg tgacgataat caaactaggt tcattgattg tttgagactt
     1201 cgcatgaggg atagcaaaac ctgacattat tcctgtagta ccttcttttt ctctttccag
     1261 aagtttttga tatacttctt tttcgtcatg agcgattcct aaacagaatg tttgctttgc
     1321 taaaaactta aatgcttctt cttgattatc taatggctgt tgtaaaaaaa tgttctcttc
     1381 atgaatgatt gtcatgataa acctcctgct cttccttctt cgataactgt acgatttgtt
     1441 gctttattat ccctcttctt gtctttatca gttactcttt tcttttggat caaacctagt
     1501 atcactccac caacgatcga accaataaga atcgctgtaa cccattggat ttttcccgtc
     1561 actacaggta acactaaaaa accgccatgt ggggctggta cttggacacc aaacagatag
     1621 gtcaagacag cagcaattga tgagcctaac atcatcgttg gtaatactcg gatcgggtct
     1681 ttcgcagcaa acggaatagc tccttccgta atatgagttg atcctaaaat aaaattaacc
     1741 aaacctgcat tacgatcatt agtatcaaaa tatttaccaa acaataaagt agcaaaacca
     1801 gtaatcaacg gtggtgcaat gcaagctgct gacgcaccag ccataaaagt cgtgtttcct
     1861 tgtgtcaaaa gagctgttcc tgttacatat gccgccttat taaccggtcc tcccatatca
     1921 aacgcacaca tacagccaac aatcacgcca agaacaagtg gattcgaata ttcaaatcca
     1981 gctaaaaagc tcatcattcc ttcatttata gccttcattg gacctgccag caaagacatc
     2041 acgatgccaa taaatgctac accaataact ggatataaga cgattgcctt caagccatct
     2101 agttgtttag gtaaaattat cagtagcttt tgcaaaaata agataaaata acctgctaag
     2161 aacccagcta aaatacctcc taaaaatcca gtaccgccat tcatggctac gattccacca
     2221 acaaatccaa cgactaaccc tggacgtttc gcgatccctt ctgcaatata agctgctaaa
     2281 actggaacca tcattcccat tgctgcatcg tcaactgctt ttaccattgc cgctgtagca
     2341 ttgtattgtg catgatctgg atcagaagaa tagattcccc aaacggcaaa tgaaatagca
     2401 atcaatactc cgccagctac cacgaaaggc aacatatgag agacaccgtt catcaaattt
     2461 ttatagatgc tacgaccaaa attagcacca cttatcgctt ctggttctaa agtatcagca
     2521 gcttcattat ctgctaagac agtacctttt cctgccaatg cttcgtcaat cagccgttgt
     2581 gcatccttga ttcctgtact gacagaaaca tcaatgactc gttttcccac aaatctttct
     2641 gaatggacat ctttgtctgc ggcaataatg acagcatctg cctgtttgat ttcttcagcc
     2701 gttaattcat tctcgatccc aatttgtcca tgggtctcca ctttgatcgt gatgcctttt
     2761 ttcctagctg cttgctctaa tgcctcttgc gccatgtagg tgtgcgcaat tcctgtcgga
     2821 caaccggttg ctgcaattag ttgatatttt cccatctttc gaacctctct tccttgattt
     2881 tgatttgttt acttaattcc tccacatcca aaaaatcagt cagtccttta cgaaaagcag
     2941 tagagctgcc tgctgctatg cttttacgta acgtttcatc aagcgatcgt cgattcatgt
     3001 atcctgctaa aaaagtgcct aacatggcat cacctgcaca tgctgtattg acaacttttc
     3061 cttttggcga gtttcctcga atcaccctgt ctttcgtaaa aagaatcgct cctttctcac
     3121 cgagggataa caaaacattt tctgcgcctg actctactag tcgttgtcca taaacaaagt
     3181 aatcttcatc tgtaaccatt tttcgtccga accaagaaga aagttcttcc tcatttggtt
     3241 tcagcaaaaa cggtcgatag ggtaaacaat ccatgacttc ttgatcacta ctatcaataa
     3301 tcaaacaaat ccgactgatt tctattaaaa cctttggaga cagccctctt ggtaaactcc
     3361 cagaaacaca aagataatcc cctgcttgta atgctcttat ttgatttaaa aaattatgaa
     3421 cagctgtctg cggtatttca ggtccttgat tcactaattt gtattcttct cccgtttgat
     3481 tgacttgtgt aaatacattg atacgcgtca taccaggcac ttcaataaac tctgtttgga
     3541 tttgtttctg ctgtaaatag tcttctatat actttcctgt gaacccagca gaaaaaccta
     3601 atgccgtact tgggaaatct agcatcttta atactagtga cacattcacc ccttttccgt
     3661 ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa aggatgcatc tcatctgttt
     3721 caataaatag atcaattgcc aaattcatcg tgcatgtgta gatagacata accaacgacc
     3781 tccttgattc tattatgaaa tacgcgaaat agaaaatgtt ttcttaatcg tcccaacaca
     3841 aagaaaacct ttacatttat aaactcgtcg tgcttttttt aactaacttt tggtgaaaga
     3901 aggaaagaat atgaatcaca aattaagtga aacagagcag tacctctgga actttatcga
     3961 acacctcatc ctagagatcc ctaactattc catcgtgaaa ctaagtgaac aagcaaatgt
     4021 ctcaacagcc accattgtgc ggacaatgaa aaagaaaggc tatgaagggt ttacttcgtt
     4081 taagcatcat ctaaaagaag gagaaaacaa gaacatcaat ttttcatttt tagataaagt
     4141 agacaaagga atccgccgag ccattctaaa aaatgaacaa gaagtggtac gtacaatcaa
     4201 tatgttggaa atcgggaaca ttgaagatgc aatacaaaga atcaaatttg ctgaccgggt
     4261 ttttgtcttt gcccgaggat tttcagaaat gattggccaa gaaatgctgg taaaacttca
     4321 attaacggga aaaaattgtc agatgcatac tgatccagaa attatcaaaa gcatcagtcg
     4381 gaaactaact aagaaagaca gcgttatttt tgtttctttg aacggtgaga aaacaaataa
     4441 aaacatcaaa aataagattt atgtctttat ttttcaattt tttaaat
//
LOCUS       DADJYG010000167         4475 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.173, whole genome shotgun sequence.
ACCESSION   DADJYG010000167 DADJYG010000000
VERSION     DADJYG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.173"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            323..1054
                     /locus_tag="JZ382_002409"
     CDS             323..1054
                     /locus_tag="JZ382_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAY2428555.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="JZ382_002410"
     CDS             1199..3226
                     /locus_tag="JZ382_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAY2428556.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="JZ382_002411"
     CDS             3334..4431
                     /locus_tag="JZ382_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAY2428557.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DADJYG010000168         4461 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.174, whole genome shotgun sequence.
ACCESSION   DADJYG010000168 DADJYG010000000
VERSION     DADJYG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.174"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            574..1008
                     /locus_tag="JZ382_002412"
     CDS             574..1008
                     /locus_tag="JZ382_002412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428558.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="JZ382_002413"
     CDS             2298..3221
                     /locus_tag="JZ382_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428559.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="JZ382_002414"
     CDS             3237..3776
                     /locus_tag="JZ382_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAY2428560.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DADJYG010000169         4405 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.175, whole genome shotgun sequence.
ACCESSION   DADJYG010000169 DADJYG010000000
VERSION     DADJYG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.175"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            157..1863
                     /locus_tag="JZ382_002415"
     CDS             157..1863
                     /locus_tag="JZ382_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAY2428561.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2021..2923)
                     /locus_tag="JZ382_002416"
     CDS             complement(2021..2923)
                     /locus_tag="JZ382_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2428562.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2920..3624)
                     /locus_tag="JZ382_002417"
     CDS             complement(2920..3624)
                     /locus_tag="JZ382_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAY2428563.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3655..4296)
                     /locus_tag="JZ382_002418"
     CDS             complement(3655..4296)
                     /locus_tag="JZ382_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAY2428564.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1351 a          947 c          761 g         1346 t
ORIGIN      
        1 tacctaaaaa tttggatttc aacgatagaa acactgtttt ttatgtaatt agtccaagat
       61 ttttctagac ttttgcttta ctattcggcc aaattggttt ttcttctttt tttcgttata
      121 atgaatatac ttatgaaaag aaatgaggaa tgaaccatgt cctttgatgg tgtatttacc
      181 cacgcgatgg tcaacgaatt gcgtgaaacg ttactatctg gccggatctc gaaaatccat
      241 cagccttatg aaaatgaaat cgtgctggtg attcgttcac gcggcaaaaa ccatcgatta
      301 ttattatctg cgcacccaag ttatgctcgt gtacagatta cccaaatcga ttatcagaat
      361 cctgataatc cgcctaactt cgtgatgatg cttcgaaaat atttggatgg cgctatttta
      421 gaagacatcg aacaaatcga aaatgatcga gtgatccatt tccactttgc taaacgaaac
      481 gagttgggtg atctacaaaa tatcatattg atcgttgaat taatgggacg tcatagtacg
      541 atcgtcttag ttaatcgaga aactggaaaa atactggatg cgatcaaaca cattggaagc
      601 tctcaaaaca cttatcgttc tttgcttcct ggtgtagaat atgttgctcc gccaaaacaa
      661 gaggtcttga acccatttag ctccgagaaa gaaaaaatct ttcagcggct ttcccaaact
      721 gaactagatc caaaagcaat ccaacgtcag tttcagggaa tcggtttcga tatcgctcaa
      781 gagctgacca aaagattact ggaacgtcct aatgaaaaga tggttgtatg ggacgaattt
      841 ttctcagcca tcagccatca gcctattcct actttttacg aaacggaaaa taaagatttt
      901 ttcacaccga ttgcttatca ggtattgtcc gaacaggcat ctgctgtaac agcttatcca
      961 acactcagtc aattattaga tagctattat cacgaaaaag cagagaaaga tcgggcaaaa
     1021 caacaaggcg gagaactgat tcgaaaaatc gaaaatgaac taaaacgaaa taaaaataaa
     1081 ttgaaaaaac gtgaacaaac actcaaagaa tccgaaaacg ctgagaatta ccgtagaaat
     1141 ggcgaactac ttactacttt tctgacacaa gtaccacgcg gcgcaaaaga agttgtttta
     1201 cctaattatt atgaagaaga tcggccaatc aaaatagcac tagatccggc attgacacca
     1261 aatcaaaatg cacagaaata tttccatcga taccaaaagc tgaagaacgc tgttaagctg
     1321 attggcgaac aaatccaaga agccaaagat gaaatccaat atcttgaatc tgtcttatct
     1381 caactggaaa ttgccggccc aatggatatc gaagcaatta aagaagaatt gacttcagaa
     1441 ggatacctga aaaagaagaa cttaaaaaaa caaaaacgta agaaaccatc tcagcctgat
     1501 caatactttt cttctgacgg aacgctcatt cttgttggta aaaacaatct tcaaaatgac
     1561 cagctgacaa tgaagacagc taaaaaaacc gactattggc ttcatgcaaa aaatataccc
     1621 ggttcgcacg tcatcattaa aagtgacaag ccctcagatg aaacaattac agaagctgcc
     1681 gagttagctg cctatttttc aaaataccga tactctgccc aagtgccggt tgatcttgta
     1741 caggtaaagc atatccgaaa accaaatggc gccaagcctg gttatgttat ttatgaaaac
     1801 caaaagacga ttatcgtgac accagaagaa gaaaaaatta cagctatgaa acaaaacagg
     1861 taatcaatga gtgtttctag aatcatattt gcgtactttc ccacaacagc aattagttat
     1921 aaattgtttg ctacaaaaag aggagttgtg acaaaattct gtcacaaccc ctcttttttg
     1981 atcaacggtg ttcaataaac agcgttctca tctgcttttc ttagctctgc tggtccagta
     2041 accgagtaag ctctttgata aatctttgag cgatttcact gagcggacgt ttcgccgaat
     2101 aaatgatata aaattcattt agttcgcctt ctgccaaagg gattaaccgc aagtgctcgc
     2161 gaatccctcc aatcacaata ccagatcctg aagcgtaagc atcagtttca agaacgatat
     2221 tcatcaaagt acctcgatca ctcgtataaa tcactgtttc ttgatcaggg atctcgataa
     2281 ggtcttcata aaaatacggg aaattatttc catcctgtgt aaaacgaact tgaggataag
     2341 ataacaattc ttctttggtg atctctgcgt gttcagctaa tggatgtcct tgccttaaaa
     2401 aaatatgcgt tttgaatttt ccaatgctgc ggccacttag ctctccttgt tgtaaatatc
     2461 ttctgatacc agcttgatta tgctcattca tataaataat gccgatctca ctgtgctgct
     2521 ctttgacttc ttcgatcact ctcgctgttg tcgtctccat gatccgaaat tgtttgatct
     2581 cgtctttgaa ttcctcgagt agctgtgcgg tcaaaggacc taagaaatca taatgctggg
     2641 cagcgatcga aaaattgtct ctcttttcac tcgttagata gtgagtttct aatgtatcaa
     2701 gctggaccaa tattttctcg gcacgattta agaagtcttg cccttcttct gttaaatatg
     2761 cccctcgatt cgttcgagta aacagcgtga tccctaactc ggtttctaat tctttgatac
     2821 cagctgaaag acttggctgg gagacaaaca ttttctttgc cgcttctctg aaactaccat
     2881 tgtttgctac agcgaccacg tatttcatct gctgaatatt cattgctcaa actcctcgaa
     2941 ataataaatt tctcttccat tcaagggctg gattggtcgg ttgttcggtc gagcatactg
     3001 accatcgaag gggagaagaa actcttggtt cagttttcct acatgatcca ttacaaacca
     3061 ttggattggc ccgctggtag gcggagttgt caatgagcca atataatgga aatggctttt
     3121 ttgctccgga aagaacacat ccggatttac ccaatgctca tgtttttcta agttccaact
     3181 ttccccattt tcttgattat acagccaacc ttcctgtacc attagctggt acatgacacc
     3241 cacaactaga ttctcttttt tgctgttcat atgaacaaaa tgaaattcaa tatcttcctg
     3301 ttttccgttg atgatatgtt cagaaggcaa gtggaaatga atatctgtca aataatagcg
     3361 ttctttcttg aactctacat aactgagttg gtcaaaaggt actaaatgaa tcgtgttttt
     3421 gaattccttg tcagtaaacc gttgcttttg ataatgaaag gaaagcgtgt ctccttctat
     3481 tttggtttct tcccccttgg tcaaatgaat aggcgactgc aatggaaatt ccgcacctct
     3541 cgcaaaccaa tcacataaac tcgcccaatt ttccggacct ttgtctccag aatagcccca
     3601 ttcgacatcc atattctttt tcaactatac ttccccctgc atctcatatc atttttattt
     3661 ttcttctttc tttacaatcg ttgagacggt ttctaaaaac gcctcttttc cataaaattc
     3721 ctggttcaat agttccggaa atatctcttt aaagaaatac tgaacagaag gccgatgttc
     3781 aaagctttta atcgtagtca ctgcctcaat gaacatctcc ttataaactt tttccgggtt
     3841 atttttgatg atttcatcaa aagattcata aagaaggtca accttatctg aaatggctaa
     3901 tagttctcct tctacggttt catcttttcc ttcgaacaac cgacggcgat aaacttcttg
     3961 gaactcagct ggaatctctt ggttgatgaa ttcgtccgtc atcttttctt caactgtttg
     4021 cagcatccct cttaactctt tgcttgcgta tttcaccggc gttttgatat caccaataaa
     4081 acgctctgtg tagtcatgat tcaatgattt ttcatagagc agtttccaat cgatcggttg
     4141 tccattcgct tcttcaatgt ctcctagaac ttgcgcaata gatgctacac gatacgaatg
     4201 tgctgcaact gtatgctctg tatatttaaa aaatccaggt gcccttgtga tcgtctctaa
     4261 attgttcaat cctaaaataa attcattcaa ccccataaaa gttacctccc tttgagattt
     4321 ctttttccga atatcatatc attaaataac tgatattcgc aacatttccg ctctttacca
     4381 tttttgaaac agctccctta ccaaa
//
LOCUS       DADJYG010000170         4383 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.176, whole genome shotgun sequence.
ACCESSION   DADJYG010000170 DADJYG010000000
VERSION     DADJYG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.176"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..310)
                     /locus_tag="JZ382_002419"
     CDS             complement(<1..310)
                     /locus_tag="JZ382_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428565.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     T"
     gene            complement(518..1444)
                     /gene="glsA"
                     /locus_tag="JZ382_002420"
     CDS             complement(518..1444)
                     /gene="glsA"
                     /locus_tag="JZ382_002420"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY2428566.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1467..2894)
                     /locus_tag="JZ382_002421"
     CDS             complement(1467..2894)
                     /locus_tag="JZ382_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2428567.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2925..4139)
                     /locus_tag="JZ382_002422"
     CDS             complement(2925..4139)
                     /locus_tag="JZ382_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAY2428568.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1380 a          923 c          775 g         1305 t
ORIGIN      
        1 tcgtgaatat accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca
       61 gaaactgtgt gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct
      121 tctctcttac ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt
      181 gaaggatcgt tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt
      241 ggaattcaaa taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat
      301 tttccagcat tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg
      361 taaatcgtat ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa
      421 gaaactggga caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag
      481 ccgtttggac ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta
      541 gttctttgac tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta
      601 gtgccgggct gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc
      661 ctacaccgct ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca
      721 aaccagcagt tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt
      781 tgccattcca aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg
      841 tagcggtgac attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc
      901 cttcgatcat ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc
      961 ttgattcaga atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt
     1021 tcataaatga caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg
     1081 ttgttgcaat cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt
     1141 ccatattcat gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga
     1201 ctttttctat tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt
     1261 cgatggcaaa gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat
     1321 aaatcgccat acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca
     1381 cttgtcctgt ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt
     1441 ccatttgaat aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat
     1501 gcgtagatcg taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca
     1561 taaatcccat gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca
     1621 aaaagtgaag tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga
     1681 acctgatagg ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa
     1741 aacataaagt acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa
     1801 agattattcc ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca
     1861 tttgcgatca tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga
     1921 agtaatcctt cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca
     1981 atttctccga tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga
     2041 tttggatcaa tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac
     2101 aaaccatgtt gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt
     2161 gccagaataa ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca
     2221 tttgcatgac tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca
     2281 accagcgtag gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt
     2341 agtggagcgc ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac
     2401 aagataccag ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac
     2461 tgtgagagta aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg
     2521 ttttgtatag caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg
     2581 gtcaagtagc caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt
     2641 tttcccaatg tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt
     2701 gccatctctg ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag
     2761 aaaaatacta agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc
     2821 attgagatgg tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt
     2881 ttgctttgtt gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg
     2941 ccaaagaaga aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta
     3001 aagtgcatcg ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg
     3061 atgacaccaa tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg
     3121 ctgttagatg caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca
     3181 ccccacacat caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga
     3241 cagacgatac ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca
     3301 caaggcgtaa tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag
     3361 ccatttcctt ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg
     3421 aatgttgaac caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta
     3481 aaaccgaacc agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta
     3541 tgctgacttc tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa
     3601 gagccgaatc cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc
     3661 tgatccaaaa atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc
     3721 caaaaaataa caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc
     3781 atcaaaggaa cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga
     3841 atcgttgcac catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct
     3901 gccgggtttc caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat
     3961 acccacataa tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata
     4021 ttattcttcc gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc
     4081 attgctgtac ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt
     4141 tttttcgcct cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct
     4201 gtttttttga attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg
     4261 ttgtgctctt tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat
     4321 agaaaaccgt ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
     4381 gaa
//
LOCUS       DADJYG010000171         4364 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.177, whole genome shotgun sequence.
ACCESSION   DADJYG010000171 DADJYG010000000
VERSION     DADJYG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.177"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(39..935)
                     /locus_tag="JZ382_002423"
     CDS             complement(39..935)
                     /locus_tag="JZ382_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2428569.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(928..1608)
                     /locus_tag="JZ382_002424"
     CDS             complement(928..1608)
                     /locus_tag="JZ382_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2428570.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1609..2391)
                     /locus_tag="JZ382_002425"
     CDS             complement(1609..2391)
                     /locus_tag="JZ382_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2428571.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2384..3301)
                     /locus_tag="JZ382_002426"
     CDS             complement(2384..3301)
                     /locus_tag="JZ382_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2428572.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3465..3788)
                     /locus_tag="JZ382_002427"
     CDS             complement(3465..3788)
                     /locus_tag="JZ382_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2428573.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1281 a          951 c          760 g         1372 t
ORIGIN      
        1 aatacatttt tctactagtt ctcactaatc ttgtataatt aaaccctccg caacttgacg
       61 gagatggaga agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc
      121 aatttttttg acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt
      181 tcggtataaa aacgctggaa gactttttca acagaggttt gcgttcctgg tttgatagga
      241 ttggccactg ttaatcgaat gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca
      301 tctgcataac gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga
      361 tctgctaata cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca
      421 gaaaattgtt cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta
      481 tggagatcca tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc
      541 atactgagta atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc
      601 gtatcctcta cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg
      661 agatcatgag aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct
      721 tgtccttttt gttcattttc atccaaccat gtattcagtg catctactag gtccattaga
      781 ttcttttctc gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg
      841 gcgattttct tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata
      901 aagataacaa cgagaagcaa cagccattta atcaaatcta aaccccactc cccacaccgt
      961 atgaatatac acagcatgtg cctttgctag cttccctcga agattggaaa tatgcatatt
     1021 cactgttttc tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg
     1081 cgaaaatact tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt
     1141 caaatggaca gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc
     1201 cttgtaatag atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg
     1261 gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt ttcgtcacat agtcatccgc
     1321 tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat
     1381 cggaacattc gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag
     1441 catgagatct agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt
     1501 acctgaataa gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaggaatg
     1561 aacagcttca tcgtcttcga taatcaaaat agtttccagc aggaccacct acttaatgtc
     1621 agatttttgg aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac
     1681 cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag
     1741 ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc
     1801 gggaatgaag gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact
     1861 tctagtgatg aatgtcaaag ccactggaac agataatgtc cctaatgtca gcagaagcaa
     1921 tacaggaaat acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa
     1981 tccgaaagca agtgtgtaag caagcatggc tagtcccgtg atcaaaacca cgccaagtgt
     2041 tgtgatcaaa agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa
     2101 agataacgta ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa
     2161 tgctgcgaaa gccagaagaa tccatgcagc cattttttct accattaaaa tccctgttgc
     2221 accgttgatg actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata
     2281 ccattcgttc caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat
     2341 tttacggctc ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc
     2401 ctccagttaa ttgtaagaaa taatcttcca gtttttgccc aactaggtga ataccatcca
     2461 caggaacttt tgcttctacc aatgcttgat tgatggctgc tacttggtca attccttcaa
     2521 aaagctggat aacacggcta gattgtacaa aataatccct ataaccaaga gagtcgagaa
     2581 ccgttactgc ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg
     2641 ccaattcttc tttcgataat tctttgatca agtgtccttc atgcaaaata ccatagtgag
     2701 tggcgatctg tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt
     2761 cttttaccaa acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat
     2821 tcgtcggttc atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta
     2881 aacgctggcg catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc
     2941 gcatcatttt cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga
     3001 tacgtaggtt atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac
     3061 ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat
     3121 caatcgagat caaacccata atagttctca taaaggtaga cttacccgct ccattttccc
     3181 caatcaatcc atagatcatt ccacgtttga tttcgattga tactttatct aatgctttgg
     3241 cagcaccgta ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca
     3301 tacttatcct tctttcttat tgatgagtga agtatagttg acgggtataa agaaagcagc
     3361 cagaaatgta taaagaaagt gtaaattttc caattgaatg tcaaaaaaaa caatcggaca
     3421 atacgtgtct ttcctattat caggcagttt gttatacgct tgtatcagtt tcctgattct
     3481 ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac tgaaaatttt tgcttctttc
     3541 agatcaagct tgagtaattt taccagaccg gttataagga taactatagc aagactccct
     3601 cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca
     3661 ccaaacagtg ctaatccaag tggactggcc accatggtaa tcgttgttat cgttgacata
     3721 agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc agaccatata aaaagcaaaa
     3781 gaaaacatta atccttaaaa acggacttat gaaaaataat acatgccgtt tgggatttac
     3841 aattagctac caaataagag gttatgacaa aagacttttc acaatccctt cttccgaata
     3901 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatttatcaa acatgagaag
     3961 caatttttgt aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcagaa
     4021 gcggataaat aatattgttt gatgatactt tatctttttc ccgtttgttt atagatcgaa
     4081 tgatattata gaccaagaat cctacctatt aacatagcta aaaacacaaa aaaggcactt
     4141 tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt aactgcgctc agcattgctt
     4201 cgcccagtac gattctcgta actcgtgaac tcgaacgata atcgcaatta acgttttgag
     4261 aactgtgaag ctttacgggc tttcttaaga cctggttttt tcttcgtatc attgaataag
     4321 tttgaaaaac cttgatttat aaaggttttc ccgtaaatat attt
//
LOCUS       DADJYG010000172         4324 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.178, whole genome shotgun sequence.
ACCESSION   DADJYG010000172 DADJYG010000000
VERSION     DADJYG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.178"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..281)
                     /locus_tag="JZ382_002428"
     CDS             complement(<1..281)
                     /locus_tag="JZ382_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAY2428574.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="JZ382_002429"
     CDS             complement(730..1272)
                     /locus_tag="JZ382_002429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428575.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="JZ382_002430"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="JZ382_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="JZ382_002431"
     CDS             complement(2359..2721)
                     /locus_tag="JZ382_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428576.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="JZ382_002432"
     CDS             complement(2725..3033)
                     /locus_tag="JZ382_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428577.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="JZ382_002433"
     CDS             complement(3042..3461)
                     /locus_tag="JZ382_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428578.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="JZ382_002434"
     CDS             complement(3454..3687)
                     /locus_tag="JZ382_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428579.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="JZ382_002435"
     CDS             complement(3684..>4324)
                     /locus_tag="JZ382_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAY2428580.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DADJYG010000173         4282 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.179, whole genome shotgun sequence.
ACCESSION   DADJYG010000173 DADJYG010000000
VERSION     DADJYG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.179"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(37..1257)
                     /locus_tag="JZ382_002436"
     CDS             complement(37..1257)
                     /locus_tag="JZ382_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAY2428581.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="JZ382_002437"
     CDS             complement(1277..1882)
                     /locus_tag="JZ382_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAY2428582.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="JZ382_002438"
     CDS             complement(1931..2863)
                     /locus_tag="JZ382_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAY2428583.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="JZ382_002439"
     CDS             complement(3284..4144)
                     /locus_tag="JZ382_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428584.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DADJYG010000174         4239 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.180, whole genome shotgun sequence.
ACCESSION   DADJYG010000174 DADJYG010000000
VERSION     DADJYG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.180"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..130)
                     /locus_tag="JZ382_002440"
     CDS             complement(<1..130)
                     /locus_tag="JZ382_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY2428585.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMF"
     gene            complement(260..844)
                     /locus_tag="JZ382_002441"
     CDS             complement(260..844)
                     /locus_tag="JZ382_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2428586.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(1175..1408)
                     /locus_tag="JZ382_002442"
     CDS             complement(1175..1408)
                     /locus_tag="JZ382_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAY2428587.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(1423..2373)
                     /locus_tag="JZ382_002443"
     CDS             complement(1423..2373)
                     /locus_tag="JZ382_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAY2428588.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(2370..3212)
                     /locus_tag="JZ382_002444"
     CDS             complement(2370..3212)
                     /locus_tag="JZ382_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAY2428589.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(3214..3933)
                     /locus_tag="JZ382_002445"
     CDS             complement(3214..3933)
                     /locus_tag="JZ382_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAY2428590.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1433 a          770 c          523 g         1513 t
ORIGIN      
        1 gaaacatcaa tactaaaact gtagcaataa tgaaacaccc cacttgaaat atatttccct
       61 ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga
      121 tttttgacac tctttcacct ccaaaaaata agatcgcact gttatcttaa gctgataaaa
      181 atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa gcataactga gaacaattct
      241 caatgcaagc tttttttaat taactcagta attcaatcca cccaggaaca gaaaattgat
      301 acaatcttct ggttaatagc atagtctttt cttttgaata gctatgcttc aatatatcat
      361 agaatgtagc gtagaatgag taaatgtaga agtggatttc ttcttcatta aagactaaat
      421 tttgattgat cattccgata gtctttaact tatctagaca acagactgtc tttttcgtca
      481 caaatgatgt aattttttca acaatatctt gatatttggt tccagctgat tggaataaaa
      541 tcaaatcgaa tatacgttta ttctcaaaaa tataattaat aaatggatct aattcattga
      601 agttctctct ccaacttttc tctatatcta tattttctaa gtttaagagg tatttggtaa
      661 actcttcatt atacatatta ataagcgcat taatatgcgg ctgaataatt tcttcaaata
      721 acgcttcctt agaagtaaaa tgtttataaa aagcacccgt agttacgcct gcggtcaggc
      781 acagttgcct tatcttagtt tgctgaaatc cttctttttc aaaatggctt agtccacttt
      841 ctattaactt ttggtgagtt ataatataat ctctggtttc tatgggtact tcctcctaat
      901 aagatgaatt aactttatta tcttacacag gacttgaagt ttcaatcgtt aacgtttatt
      961 actgaactca ttccttagag ctaaactaat gagaaagagc agaataactg atacaaaaag
     1021 caatcctaca agaattaata gactcatcta aatcattcac cttttctaaa ataatccgag
     1081 aaagatttaa aaaaattata tccagctatt aggataagtc ctatcataat cactccaata
     1141 gccattttct tatcctccct aaaaataaag tcttttactt aaaacctttt ttgtaatcgt
     1201 caataaattt tttcccttta tcagttaatt gaaatcctat atctaaatag tcctcatatc
     1261 taagtttagc cagcatctca cttacagatg ctggactaac acttagaact tttgctaaat
     1321 cattattttt taatttcttt tttctttttg ataattcctg aattgctagt aaataaatta
     1381 gtatagtagt cgttttaatt tgtaccatac ctaccctcca aattatttcg aaaggccttc
     1441 agcaatttta tccaggttcc atttcatcat cgcatagtaa ctatcaccat cttgtccttt
     1501 ttctgcaatt gaatcagtaa aaatcgttga atagatagga atattggtat cttttgatac
     1561 tgttttcatt ggtctatcat ccacactgct ttctacgaat aaggagggaa cttttgttgt
     1621 gcgtaatttt tcaactaagt gttttatttg atctggtgtt ccttcttctt cagtgttgat
     1681 ttcccaaatg taagcagaag gcacattata cgctttcgag aaatatttaa agcatccttc
     1741 acttgtaaca atcatttttt tatcattcgg aattgaagca aatttagatt tagcttcttt
     1801 gtctagagaa tccaattttt caatatactt atctagattt tctttataga attttttatt
     1861 atcaggatct ttttccgcta actgtttttc aatattttta gcgtaaataa taccgttttc
     1921 taaatttaac caagcatgag gatcttcctt ccctttctca ctctgaccct ctaagtaaat
     1981 aacatctatg ccatcacttg ctgcaaaata gtctttgttt tcctctttgt tcgcattttt
     2041 aactaatttt gtaaaccaag catttcctcc agtttccaag ttaacaccgt tataaaaaat
     2101 caaatctgct tttgaagttt tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc
     2161 tttcccaata ggtacgatac tgtgtaaatc aattttatct tttgctatat tttcggtaat
     2221 atctgctaaa atcgaattag taactactac ttttaatttt tcgttcgaag ccactttatc
     2281 tttgtcttta ctatttattg aacatgcccc gaatataaaa agagcaatca aaatccccag
     2341 acttcccagt actgctttcc attttctcat cataacgatc tctccttctt tcttaaaaat
     2401 ttttgttttg gtgagattag aaaactaatt acaaataaga atgccgctgt taaaacaata
     2461 cttgatcctg ctgctatgtt aaaactataa ccaataaaca atccaagcaa tgatgaactt
     2521 gctccgactg ttgcagaaat aaatatcata gttttcaagc tatttgcata taaatatgct
     2581 gtagctgctg gtgtaattaa cattgcaaca attaatatag ttcccacact ttgcatagct
     2641 gttactgcta ctaaagttaa tagaaccatc aatagataat gataaaaact gacattcatc
     2701 cctattgctt ttgccattaa tggatcaaaa gaagtgatta ataattcttt aaaaaataga
     2761 actactataa ccaacactaa aaccgaaacg ccaattgtaa tccacttatc aatatcttgt
     2821 acagctaaaa tatttccaaa taaaatatga aataaatccg ttgagctatt tgcaactcct
     2881 ataaggataa ctcctagtgc taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct
     2941 tttattacac tattgctttt tataaaagta ataatgatag aagctaatag accaaataca
     3001 atcgcaccaa taaaaaaatt aatacctaaa atataagaaa ttgctactcc tggcaaaaca
     3061 gcatgagaaa tagcatcacc cataagagac attcctctta aaataataaa acaacctact
     3121 gttccagaaa caatccctat aactacagat gtaattagag cattctgtaa aaagtgaaag
     3181 tcatacagac cattaataaa attttgaagc atattattcc ccctctccaa taaatatagt
     3241 atctccatag gcttttttta aattttcttc attaaataca gattctacac ttccgtgagc
     3301 aattagattt cgattcaaaa gaataatatt atcaaaatac ttctttacct tgcttaaatc
     3361 atgatgcaca attagcactg tttttccctg atgtttcaat ttttttaacg tatccataat
     3421 tattcgttca ctaactaagt ctatccctac aaatggttca tctaaaaata tgaaatccgc
     3481 attttgagca agacatcttg ctaaaagtac acgttgaaat tgtcctccag ataattctcc
     3541 tatttgatga ttagaatatt ttcccatatc gaccttttcc aaagcctttg aaactctttg
     3601 ccattctgca tttttaattc tttgaaaaac tttcattccg gcatatgttc ccatagatac
     3661 acattctttt acttttattg gaaaagtaaa atcaatatca gctttttgct ctacatatgc
     3721 aatcttcacc agttttttat tgatatcttc attattaatg gagacctctc cctgatgagg
     3781 gataatattt aacattgctt taagcaatgt ggattttcca gcaccatttg gaccaataat
     3841 acctgttaaa gcaccttctt gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt
     3901 ataacaaaca ttaagctttt tcacttctaa cataactttc tccttctaaa tgataatgat
     3961 tatcacgatc atatggtata caaaaattat attttagtca accctaaatt ttatagttta
     4021 gatttttttc tgttgctgaa gattagaatg acaaaactat ttgaaactac gcgtatagta
     4081 agtagctttc aaaatatata aaaaaggagc gttctcaaat gagaacatgc tcctaaaact
     4141 actttattgg cgtatggaaa aattatactt gaagtggata gcgggttgaa taagttgata
     4201 agcatcaaaa catttataaa aattattcaa gactatgtc
//
LOCUS       DADJYG010000175         4203 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.181, whole genome shotgun sequence.
ACCESSION   DADJYG010000175 DADJYG010000000
VERSION     DADJYG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.181"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..354)
                     /locus_tag="JZ382_002446"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="JZ382_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="JZ382_002447"
     CDS             complement(614..1162)
                     /locus_tag="JZ382_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428591.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="JZ382_002448"
     CDS             complement(1163..2020)
                     /locus_tag="JZ382_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY2428592.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="JZ382_002449"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="JZ382_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="JZ382_002450"
     CDS             complement(2582..2911)
                     /locus_tag="JZ382_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY2428593.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="JZ382_002451"
     CDS             complement(2988..3260)
                     /locus_tag="JZ382_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAY2428594.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="JZ382_002452"
     CDS             complement(3260..3523)
                     /locus_tag="JZ382_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY2428595.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="JZ382_002453"
     CDS             complement(3629..4045)
                     /locus_tag="JZ382_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428596.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="JZ382_002454"
     CDS             complement(4051..>4203)
                     /locus_tag="JZ382_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAY2428597.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DADJYG010000176         4183 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.182, whole genome shotgun sequence.
ACCESSION   DADJYG010000176 DADJYG010000000
VERSION     DADJYG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.182"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            448..1416
                     /locus_tag="JZ382_002455"
     CDS             448..1416
                     /locus_tag="JZ382_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY2428598.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1427..2242
                     /locus_tag="JZ382_002456"
     CDS             1427..2242
                     /locus_tag="JZ382_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY2428599.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2310..3020
                     /locus_tag="JZ382_002457"
     CDS             2310..3020
                     /locus_tag="JZ382_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY2428600.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3056..>4183
                     /locus_tag="JZ382_002458"
     CDS             3056..>4183
                     /locus_tag="JZ382_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY2428601.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1398 a          628 c          890 g         1267 t
ORIGIN      
        1 tttaatcagt tttaagacca taaattgcgt actaaaagaa attctgcaat tcaaaataaa
       61 gcattttaag ccattttcaa atttaagtgc acccatctac ccgtcaaatc actttatttt
      121 ccactccagt agtttcctaa ttcaactgtg tgcaaaaacg atttcttcta aacaaatcaa
      181 gacaaactta taaaccttca tcccagcacc ataattattt aaatcatagg catttttcga
      241 aattagtata tgtactcaag tttcactgca gattatttat attttttaga gaaataaatt
      301 tttcctattg acaaacacaa agaaagcgga tacaatgcaa ttatgtcaca tgacgttata
      361 tgacgttatg tgatatcttt acatttaatc agttactttt attttggata aaattaacat
      421 ttttaaaatt ttaaggagga aatgttgatg gatgtagcaa aaacggttaa cgcaattgta
      481 gaaaaaaatg atggagagat ttctggaatt tatttggtgg cttgtggcgg ttcgctggta
      541 gatatgtatg tctcagatta ttttcttaaa gccgaagcat cgaagtgtat tacaggctta
      601 tatacagcga acgaatttaa ccatgtaacc ccaaagcaat tagatgataa atctgttgtc
      661 attctatgtt ctcatggagg aaatacagca gaaacggtaa aagcaggaga agtcgctcaa
      721 gctagaggag ctgtgactgt tggtctgact cataataaaa ctgcggattt attaaaggtt
      781 tctgatcata gtttccttta cgaatgggga gatgatgcaa aaatagtcaa taatcctatg
      841 gccatcattc ttgatttaac tgtatctttg gtcaatgtta ccgaaacatt tgaacattac
      901 ggtaaattca gagaaggcat gaaggttatc gataccgtaa ttgaaaatgg cattaagcag
      961 gtgcaggatc gtattaaagt gtttgctgat aagtacaaag atgaaacaat gttttatatc
     1021 ttaggtagtg gaccatcata tggacatgct tatggttttt caatttgctc tttgatggaa
     1081 atgcaatggc ttgattcaac ttctgttcat tctggagaat tcttccatgg accttttgaa
     1141 gtaacagaca aagatacgaa ttttattctc ttgatgagca ttggacgcac tcgtgcttta
     1201 gatgaacggg caaaacgatt ccttgataaa tatgcagaaa gagtagaaat cgttgatgca
     1261 aaggaactag gattggatat gatatcagat gaagtttctg aattcttcaa tcccatttta
     1321 ttctacagta ttctttcaga atatcgttca gctttagcag atgtacgcaa tcatgattta
     1381 gatgttcgtc gctatatggg gaaagtggag tactaggagg attaaaatga aggtattagg
     1441 cttaggtgat aacgtcgtcg ataaatatga aaatctggga attatgtatc ctggaggaaa
     1501 ttcattgaat tttgcagttt ttgcaaaaaa actgggacat gaaagtagct ttatgggagt
     1561 ttttggctca gatgaggagg cagctcatgt tttatcagca ataaaagaaa taggactgga
     1621 caacagtcac tctcgctttg aaaaaggcga gaatggctgt gccagagtga agattgatga
     1681 gggcgaccgg attttccttg gcagcaattc gggaggtgtt actcgtgaat acccgattca
     1741 gcttactgaa gaagacagag aatatttaaa ccagtttgaa ttgattcata tgggattata
     1801 tagccatgtc aatcatctat tagaagagtt acaaaatgtt tctggaaaga tttcttatga
     1861 tttttctgac gactttacgg aagaagaggt tcagcgggca attgataaag ttgattttgg
     1921 cttcttttct atagaagact tttccgatga agaagtaaag cagtttttga tgaagcattt
     1981 ttgtgagcga aatgatgtat tgattgcaac ccgaggagaa aaaacggcta ttggttatga
     2041 tggagcaaag ttttatgaag tgaaccccgt gctagaagaa ccagttgata caatggcagc
     2101 gggagattct ttcttaacag ctttcctact ttattttctc gaaggggacg atatagtcac
     2161 cgcgatgaaa aaggggaatg aatttgctgg gaagtcttgt ctggttgacg gttcgttcgg
     2221 ctttggtatt cattacgatt gagcttgttt taaattcctg catttgataa cctaaagaaa
     2281 gaaatcccgg taaagtggag gttaaaaaaa tgctggatac aaatgcacaa ttaccattgt
     2341 acttacaatt aaaagaaata ttaaaaaata aaatcaatac aggggagtat cccgagaata
     2401 cgaaaattcc cgctgaatca gagctgatag aaagttatga ggtcagccga atcacagtga
     2461 gaaaagcagt agaagaactg gtagaagaag ggtacctact taaaaaacaa gggaaaggaa
     2521 cttttgtcaa ccggcataaa gtttttcgta aaattgaata tattatgggt ttcagtgaga
     2581 gttgtaaatc taatggtttt acaccgacta gcattgtttt ggaacgcaag atcattccag
     2641 caactgaagg gatggcaaaa aaactcgata tcgctcaagg tgatgatgtt ttatatatcc
     2701 agagaaagcg aatggcggat aacatcccca ttatgttaga aaacaattat ttttcttatg
     2761 aaagatatag tttcttaatg gaggaagagc tgactgattc tttgtatgaa gtacttaaag
     2821 tccaaggtac cttagccatc aatcctgggg aaactgcact tgaaattgtc acagctgatg
     2881 accaaattgc caaaataatg gaagtagctg ttgggacgcc ctttttcttt atggatactt
     2941 taatcaacga tcaaaacaac ataccaattc atgtaggtaa tcaatattac ctgggagaag
     3001 tatatcgttt ttacttatga tttcctgttt ggatttcaag gagtagattt tatttatgaa
     3061 gcaaatattg cggtcaaaaa tgatttttga tggagagcag ccacctttca aaggttatat
     3121 tgtaatcaat gaagggatca ttgaaaaggt ggaaaacaac tggaattaca gtgatctatt
     3181 aaatgaaaac acggaattaa ttgcttatga tgatcaattc gttatgccag caattcatga
     3241 taatcatgta tttttcagtg gttatttggc tatgaatgct ggcatagatt taagcgatac
     3301 atcttcagct aacgaggctg tagagcgtat taaagctgta atgaaccata aaaaagcaga
     3361 tgaaccaatt tatgctcatg gctgggatgc tgccctgtgg aaaactgacc catcagaaga
     3421 gctattgaat gaaataaacc actttggtcc aatcacagcg atcgacaaaa atcgcagcca
     3481 ttgttggatg aatgctattg ctaagcagac ttatggtttt acagaaaaag aaaccagtgc
     3541 tgaaagtcga gtattattaa ttaaggaaat gctagcaaac aaaaagcttt tagcagaggt
     3601 ttacgctgaa tttgaagcat tactgctttc aaaaggtgtt atatcaatga aagaaatcgt
     3661 ttttgatgac gcagaatttc tttctaatat acctcagagg aaaatactta gtaacctata
     3721 tgtgcaggca gtccaagaac ctctacagca tgaacttcta tatgagtata aggaaaaagt
     3781 tttccctgaa aaggtccgct ttggtggagt gaagatcatg gtagatggtg ttgttgcgga
     3841 tagaagtgga gatatttacg gtgtttatca gtcaggagta gcttcacccg agattgacta
     3901 tgaggcaatt aaagcagaag ttgctgcatt caatgaagta gagattcctt gctgtttgac
     3961 aacggaagga gataaagcgg gttataaagc tgcgtcaatt cttgtcgaat ttggaaagcg
     4021 gttacctgaa ggcgttttta attcgattag tgatttggaa atggttactc aaaagacagc
     4081 agaaaaaatg aacgaaggac aagtagtagc agaggtatat ccacaaattc tcggcttgaa
     4141 tcaaacgtta gaagaggctt acatgtcaac tgttataaaa aat
//
LOCUS       DADJYG010000177         4182 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.183, whole genome shotgun sequence.
ACCESSION   DADJYG010000177 DADJYG010000000
VERSION     DADJYG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.183"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..280
                     /locus_tag="JZ382_002459"
     CDS             17..280
                     /locus_tag="JZ382_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAY2428602.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            274..624
                     /locus_tag="JZ382_002460"
     CDS             274..624
                     /locus_tag="JZ382_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY2428603.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(648..1262)
                     /locus_tag="JZ382_002461"
     CDS             complement(648..1262)
                     /locus_tag="JZ382_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428604.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1711..3036
                     /locus_tag="JZ382_002462"
     CDS             1711..3036
                     /locus_tag="JZ382_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY2428605.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTERTRSQRRKKLAQMIQEKIKEELGLIA
                     TVGIGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYSIKSRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRNVGITYSKLVYTDSLQLDLFSDPQKQIDEAN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            3029..3379
                     /locus_tag="JZ382_002463"
     CDS             3029..3379
                     /locus_tag="JZ382_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428606.1"
                     /translation="MINYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3376..3555
                     /locus_tag="JZ382_002464"
     CDS             3376..3555
                     /locus_tag="JZ382_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428607.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3691..3984
                     /locus_tag="JZ382_002465"
     CDS             3691..3984
                     /locus_tag="JZ382_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAY2428608.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFIYDLSLASSCNMSDVYEKLLILENEYDQFNSNQKKKKKKHID"
BASE COUNT         1475 a          634 c          800 g         1273 t
ORIGIN      
        1 gaaagaggtg tctactatgg aaatgttacc tataaaaaaa tggggaaata gtaatggcct
       61 tagattgcca aagtatatta tggaatatct tggaattcat acagaagaca aagtgaagat
      121 aatacaagaa gaagtaaacg gtcagaagcg gctaattatc gaagcggcta atatagaaaa
      181 tgaattaaca attgaacagt tatttgaaaa ttacactgat gaaagaaatc atgtgacgat
      241 tcaaaattta ggtgaagcag taggtaatga aaaatggtga gaaaacctag acaaggcgat
      301 attctattat tgaatacagc acctcgatct ggacacgaac agacaggtaa aagaccttat
      361 attgttttaa gtcatgatat tattgcggat tatagtaacg ttgttacagt tgctccaatt
      421 tctagtacaa ctagaaacta tccgctatat gtaaatataa actcagaata tgaaatgaag
      481 acaacaggaa aagtattatt agatcaacta acgacaattg attatgaagc cagagagtgt
      541 atttttttgg aaaaagcgca tgatagttta gttgaggagt tattgatgaa agttagaaca
      601 gtttttcaaa aagtaagcaa ataaaataga aacagcagca tgatcaacta ttcgtgattg
      661 accatgctgt ctttttttat tctatatacg gtttgtctcg taacattgta gtcttttgca
      721 atcttagcga tcgcaacacc tttttttaga tcctcaacga tgtttttata aacaagacga
      781 cgttggggat cttttgcatt ggcactgtat agcttaggac gtcctttata aacaccgttc
      841 gcttttgcaa tcttaatgcc ttgtgcctga cgtcgtttag attcggtacg ttcttgttca
      901 gcgatcatgg ccaaaatttg aatgatcaaa tctttaatga atttatccag gagaggattg
      961 ccaattgctt cggccatgat cggtagactt gtgatgatca gctgcacatt tttcttcttc
     1021 aagtaattga ctgaagcgat gatctcgtcg taatttcttc caagtcgatc aatagcttcc
     1081 acaataaaaa tatcttgatc tcggacgaaa tccagtgctt cttgaaaaac aggtcgatga
     1141 gtaatcgttg cgccagattg tttctcaatg aaaattctct ctgcaccgaa ttttttcatt
     1201 tcttcaatct gccgttgttc attttgatcg atggatgaga cacgtatgta tgcaactctc
     1261 aaaattttta cctactttct gcaactattt aaaaaacacc ctaatcatac acgagaaaac
     1321 cagatttttc aatgtcgcgg tcgtgtctag ttagggtata ccttatatag acaaaaacta
     1381 atgatggagg aaatagtatg agaatttttt gtaagtgtct actgataact tattctcaaa
     1441 tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa aaagtccgta
     1501 ctctaatttg cggtctctca ctcttttaga gtgagagttt ttattagatt ttcaaattaa
     1561 ttagtaaagt gatagaataa attgatcata caaatcaaaa gaagaagcag ggtgatagca
     1621 tacctatgct tcttttttat gtatttagct atttcttctt tacaatatcg aacatatgtt
     1681 tgtataatgt gaaagaatgg agtgttattt atgatggatt attcaaaaga gccagtaaat
     1741 gattactttt taatcgatat gaaaagcttt tacgcaagtg tcgaatgtat agaaagaaat
     1801 ttagatccat taacaacaga acttgttgtt atgagtcgag ctgataatac tggttcagga
     1861 ttgatattag cttcttctcc tgaagcaaaa aagcggtatg gtattacaaa tgtgagtaga
     1921 ccacgtgact taccacaacc atttcctaaa acactacacg ttgttccacc acgtatgaac
     1981 ctatatatca agcgaaatat gcaggtaaat aatattttca gaaggtatgt agcagacgaa
     2041 gatctattga tctattcaat cgatgaatca atcttaaaag tgaccagatc actgaacctt
     2101 tttacgaccg aaagaacacg aagtcaacgg agaaaaaaac ttgctcaaat gatccaggag
     2161 aagattaaag aagagctagg tttgattgct acggtaggca tcggcgataa tccccttttg
     2221 gctaaattag ccttagacaa tgaagcaaag cataatgaag gatttattgc ggaatggacg
     2281 tacgaaaacg ttcctgagaa agtatggaat attcccgaaa tgacggactt ttggggcatt
     2341 ggttcgcgaa tgaaaaaaag gctcaaccaa atggggatta tgagtattag agatcttgct
     2401 aactggaatc cttactcgat caaaagtcgt ttaggtgtaa ttggcttgca gctttatttt
     2461 catgcaaatg gtattgatcg aactgacata gcaatcccac cagaaccgac aaaggaaaaa
     2521 tcatatggaa attcacaagt gttaccacga gattatacta gaagaaatga aatcgagttg
     2581 gtggtaaaag aaatggcgga acaagtagcg atacgcatta ggcagcataa ctgtaaaaca
     2641 ggttgtgtcc atttacacat tggcacgtcg atcttagaaa cgagaccagg gttttcacac
     2701 cagatgaaga tccctataac agataataca aaggaattgc agaattattg tcttttcttg
     2761 tttgataagt attatgaagg acaagaagta aggaacgttg gaataaccta tagcaagctt
     2821 gtgtacaccg attcgcttca gctagattta tttagtgatc cacaaaagca gattgatgaa
     2881 gcgaacttgg ataaaattat tgataaaatc agacaaaagt atggttttac gtcgattgta
     2941 catgcttctt caatgttaga aggcgctaga agcattacta gaagcacact tgttggtggt
     3001 catgcgggcg gaaatggggg aattaaaaat gattaattac aatgatcgtg gacgtttaaa
     3061 gtgggcaggg ttttatttat ctgatcatac tgaagaaatt gatgccgaca atgaacaacg
     3121 aaccagtcaa aatttagcaa aagaacaaat gacgactgaa gagatttcgg aagtattgaa
     3181 cgctgcagtc ataaaaaata gattagtatg tattcaaaaa gaagaacgaa atgctgaagg
     3241 atactatcca gacgatataa ttggcaaaat attaggtagt gatgagctgg ggatctacgt
     3301 tggcaaagac aaaattcact acgacgaaat acgtcatatc gaattttacc aggataataa
     3361 atggagtcga ttagaatgaa cattatccag caatatgagc taaagtatat aacctttgat
     3421 cagctatcag aagagatttg gggttatggc caacgtttga taaatgaggt tggtgttgag
     3481 cgcttctctt tctatgtaga agctgcagca ggttatcata attttaggtt ttatatcttc
     3541 ccgttatata tataaggtat tttagggatt ctatagagtt atctctatga atcctttttt
     3601 gtttttacaa ataggagtag atactagaat aatgcgtgtg ttaaactatt attgcaaacg
     3661 caataataaa agaaagaagg cttacgcacc atgtctatca atacatttgt ttactcgcac
     3721 ccgatcaatg tttacattat caagaattta ggaatcactg tagaacaatt ttgtgagtta
     3781 tatgcatacc cacaaggaac ggttgcaagc tggattactc gacaacgtag aatcaaatca
     3841 ctacctgctt catttatata tgatctgagt cttgcttcta gttgcaacat gtctgatgtc
     3901 tatgaaaaat tattgatact agagaatgag tacgatcaat ttaattccaa tcaaaaaaag
     3961 aaaaagaaaa aacacataga ttaatattga aacgaaaaat agtatttatt ttaaaaaaag
     4021 agaaagtaga aaaagatatt tttccattat ttgattaaag ttacttttaa tcaaaactat
     4081 tgtaaaatag tacttgtttt atgtatctta aaatcaaaaa actttgatta aagattcttc
     4141 aatcaaaaaa acttaaaaac attgatataa tagcatttaa ta
//
LOCUS       DADJYG010000178         4168 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.184, whole genome shotgun sequence.
ACCESSION   DADJYG010000178 DADJYG010000000
VERSION     DADJYG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.184"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(22..1794)
                     /locus_tag="JZ382_002466"
     CDS             complement(22..1794)
                     /locus_tag="JZ382_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428609.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1748..3502)
                     /locus_tag="JZ382_002467"
     CDS             complement(1748..3502)
                     /locus_tag="JZ382_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428610.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3612..>4168)
                     /locus_tag="JZ382_002468"
     CDS             complement(3612..>4168)
                     /locus_tag="JZ382_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAY2428611.1"
                     /translation="YIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGII
                     FVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRA
                     IPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIIS
                     VLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1409 a          794 c          588 g         1377 t
ORIGIN      
        1 gttaatttaa agcctatttc atcatcgtga ttgagcttct ttttcataga cgaatttctc
       61 agatagttta gttttcttta tgagattttt gtatatttct gaccttcgat acaactcatt
      121 atgagtacct tgatcaacta gttttccttg atccatcact acaattttat ttaccttttc
      181 aatagacttc aagcgatgcg atataattag tactgtctta tcttgtatta aacgatttaa
      241 actttcctga atacgttttt cattttccgc atctaaactg gcagtaattt catctaaaat
      301 taaaattggc gcatttttca aaaaagcccg tgctatggat aggcgttgtc gctctcctcc
      361 agaaagtgaa cttccgtttt ctccgaccaa tgtatgataa ccgtcaggta gtttttcaat
      421 aaagtcttcg cagttagcta aacgagctgc ttttttcact tcctcatctg acgcttgact
      481 atttccaata cgtatatttt ccaaaatact tgtattaaac aaagttacct cttgaaaaac
      541 aatagcgatt ttgctaaaca aagaagctgg agatacacgt ttaatatcat acccatcaat
      601 taaaatacag ccttcatcat aatcatacag tctagacact aatttcaaga tactagtttt
      661 cccggaacca ctagcaccta ctaacgcagt cacttctcct tgtttagccg taaaagagat
      721 atgatctaaa attggggtat tgttatcata tgagaaagag acatctcgca actcaatgtc
      781 aaaagatttc agaggactat ctgatccctc ttggatactg gtttccttca tagctctaat
      841 tcgctgtatt ttaggagcta aataaaatat ttcaagcact gcttctttca tagaatcgaa
      901 ggaatccttt atttttattg cggctagtaa gtaaccaacg acgtagagta tagtcacttc
      961 acccgttaac agtaagtgaa cacctactag aatgactact gctaaactaa catatgagaa
     1021 aatagaggat aaggccatga ctgaaaaagt acttaattct acctttaaat gaatcctttc
     1081 actctcttcc atttttttga aaagtctgtc ttgaactttt ttagatagat taaagctatt
     1141 aatctcttgc tgcaattcta tagtttcttg aaattcttcc gagttttctc tcaaagtatc
     1201 gtaatattta gtattggctt tcgtttggga tttctttgat aacaagatta agacaaaact
     1261 aaataacgtt ggcaaaataa cagctaatcc cattttgaca ttaccaacca aaagcatcac
     1321 tgaaataaaa gggaaaaaca gagccatacc gccggcttta ggtattgcat gactcatcgc
     1381 atgctcaata ccttcaacgt cagacatgat agtttgagat aaatctgata aattatgttt
     1441 tgaaaaatag gataatggta aatttgacaa atcgtctgca atttgcactc ttaaatgcgc
     1501 actttcttta taagtactat tatataagtt ttcatactcc ctatctaaca accaatacat
     1561 gaaaagcaaa gtaacaaacg atacggcaaa ataaagccaa cggggctttg tattttctaa
     1621 aactaattcg tctatcagca tcattataat gaatgctggt accatattaa tgcagtatac
     1681 aaaaaaacta gaaatagaag ctttagttaa agcttctgcg ccctgatcac ttaaagcaaa
     1741 tttgttttta taaaaacttt tcattgtcaa gcctccaatc atttgcattc tcataattag
     1801 tgaccaattg tttatacact tgatgtttag ccattaattc attatgattt ccacgttcta
     1861 ctattttccc ttctgacaag acaagaattt catctacaga tttaatgctg gataatcgat
     1921 gagctatcat aataactgtt ttttctttca tcagatgttg aaaagccttt tgtagttcat
     1981 actcattatc tggatcaata gatgcactgg cttcatccat aataacaatc tttgcatctt
     2041 ttaaaattgc tctggcaata gctatacgtt gtttttctcc tcctgacaga taaactcctt
     2101 tactaccaat taaggtatgc tctctttcgg gaaatttttc taaaatcgat tcgcaaccgg
     2161 ctaatcttag cgcgtcaaat actttacttg ctggcgcttg aggatttgct atagaaacgt
     2221 tatcgtaaat actctgattg aatagtttcg tatcttgaaa gacaaaagca attgtgttaa
     2281 taattgtgtc ttttgaatag tgatttaaag gcttaccacc aattttaatc tgtcccttat
     2341 ccacattgta gaaacctgaa attagtttag ctatcgtact ctttccgctt cctgattttc
     2401 ctactaatgc ataaattctt ttttgttcta aaactaagtt gaaattttct aaaacacact
     2461 cttctttgta tgaaaagctg acattttcaa atgcaatgtc acaattttta atctgtgtct
     2521 cgttcccata ttttagttgg tcttgcttca tttctaaata aagggagtct agtgtttcga
     2581 ctgcatatct tgcttcaaac aagtgcatac ttacatacat tattctcatg aaagaaacaa
     2641 aaataattcc acttaaaaaa aacaacatca acaagtcaac agctaatatc ttgggagtgg
     2701 taagagaagt tataaaaaag gttataggaa ctatcaaaat gcaaactatg ccaaaaaata
     2761 gccattgaaa aagaacaaaa ggtgttttcc cactcaaaga atacttatat gcaaacttag
     2821 cataatcgtt aattgcttta ttcaaggctt taaatgatgt tactttagca ccaaatatct
     2881 ttataacttg aattccgcgg atatattcta ccgtttcaga gcttaacaca tctaacgatt
     2941 cttgatattt ttgaataaaa tttgtattct ctgtcatttt ctttaacaag aatacaccaa
     3001 ttactaacat ggcgattaga accaatccaa ttttaaagtt aatgaaaaag cctaaggcaa
     3061 ctgataaaac aggtgttact atggcttgag cactatctgg aatcaagtga gcaattgcca
     3121 tatgtgtttt agccgcgttg tcatcaattg tctttcgaat atatcccgaa gaatttaaat
     3181 caaaaaaacg aaaacttgct tctgttaaac cgctaattcc cttttttctc aaattagtct
     3241 ctaagcgaaa cccaaggata tgagaaaata agccagaaaa aatataaaat aaagctccta
     3301 aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca tctcctacaa
     3361 taattagaga ctgaagaaat ttgtaaatac tgtaatatcc atagactgtt aatacgacag
     3421 aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg acataactaa
     3481 ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc tcttattcgt
     3541 ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac agataaaagg
     3601 taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa tacaccaatc
     3661 atagataaac ttgctacaac taacactgaa atgattcccc atatacctac cgcatagtgg
     3721 atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg acgaaaacga
     3781 tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg cattagagcc
     3841 caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat aatatcagct
     3901 aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc cattaacgta
     3961 aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg gtagttatag
     4021 ctaatataaa caaatatgat tccagataat aatccaaaaa tcccggggga tatgctgtga
     4081 ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac tagggaaata
     4141 actgtagcta aaccaataaa aatataat
//
LOCUS       DADJYG010000179         4087 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.185, whole genome shotgun sequence.
ACCESSION   DADJYG010000179 DADJYG010000000
VERSION     DADJYG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.185"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            52..1353
                     /locus_tag="JZ382_002469"
     CDS             52..1353
                     /locus_tag="JZ382_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2428612.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="JZ382_002470"
     CDS             1380..1640
                     /locus_tag="JZ382_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY2428613.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="JZ382_002471"
     CDS             complement(2639..3874)
                     /locus_tag="JZ382_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAY2428614.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1161 a          663 c          857 g         1406 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagata
//
LOCUS       DADJYG010000180         4059 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.186, whole genome shotgun sequence.
ACCESSION   DADJYG010000180 DADJYG010000000
VERSION     DADJYG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.186"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..406
                     /locus_tag="JZ382_002472"
     CDS             <1..406
                     /locus_tag="JZ382_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAY2428615.1"
                     /translation="EIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEI
                     PKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAE
                     LNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            409..1305
                     /locus_tag="JZ382_002473"
     CDS             409..1305
                     /locus_tag="JZ382_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2428616.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1355..1744
                     /locus_tag="JZ382_002474"
     CDS             1355..1744
                     /locus_tag="JZ382_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428617.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1731..>4059
                     /locus_tag="JZ382_002475"
     CDS             1731..>4059
                     /locus_tag="JZ382_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2428618.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1377 a          639 c          853 g         1190 t
ORIGIN      
        1 tgaaattgaa gatttaaaaa aacaagtaga aaagctatct cataagagtt ataaagatgt
       61 ttatgatgag tattcaaaag agacagatat ttatagggaa ctgaaacgtg atgtggaggc
      121 acttaaggaa attcccaaaa atctaactga tagtgttgac aaaattgact ttggcaatgg
      181 tataaaagtt acagacgaaa ttgatgaaca ccgcggagaa ttgattaaga atttagtgac
      241 caatcgggct aagattcaaa caataattaa tgaaatgtca gatgaaattg atagttatag
      301 agcagagctg aataaatcag attggaaagt taactataat gaggtttcaa agaggtatca
      361 aagaggtatc aaaaacttag tgaagagtta tctcaagatg aattaaaaat gttacaaaac
      421 cttgattctt tatatcaatc tttatcaaaa actgaagagc gattggaaga aattattcaa
      481 caagaaataa agcttaaaga ttaccaagtt gaggttgact cagcaatgtc aagaatgaat
      541 aaatcatatc aaaatctcta ttctgagaga agtagttcct cgcgcgaaat tttttctgat
      601 attacaggaa tagaggctac tattcaacca aatagggatt tcaataatta tgttaaagag
      661 ctcagagctt gtattaataa ggaggacact tttgacagtg agtttggatc tcttattgat
      721 aagctgacaa aaaattccat tactaatcaa gatatatata atgacatact tgatataaga
      781 gttagtgggg aaagtagtat tttttcagat aaaaaattaa tcaattctat aagaaaattg
      841 aatgatgcac aagttcttga attacaaacc cttaagccaa atgatttagt agttatccga
      901 cttgaactaa atggaaaaaa agttagttta tcgaatgcta gtgctggtca aaagacatct
      961 gcaattcttt ctatgatttt ggcgtatgga aattcaccac tcatattgga tcagcctgaa
     1021 gatgatttgg atagtcaatt aattaataac ttaatcgtaa gaagtatcat tacaaaaaag
     1081 gaaaacagac aaattttaat tgtaactcat aatgcaaata tcccagttaa tggtgattca
     1141 gaatggttga ttagcatggg agattcaaaa gaaatatcgg ctgatcagtg cgggtcggta
     1201 gatgaaccca atatcaagcc aagaatatgt actgtgatgg aaggtggcga ggatgctttt
     1261 acaaatagag cgaaacgcta tggttttaaa caaattgtag aataacacta gagggtgaga
     1321 catattatta tgtccgccct ttttaggagg aaaaatgaaa aaaataaaaa gctataccag
     1381 tatctgggca gttgaaaaag tcatttacgc aatcaatgat tttcagttgc cttttcctgt
     1441 gacttttaac caaatggctt ggttcgtttt atctttactg tttgtcatcg tatttgccca
     1501 tgtgcctcca ctatctatga tagaggaggt ctttttaaaa tacttaggca taccggtggc
     1561 tgtcacttgg tttatgtcgc aaaagacgtt tgatggcaag aaaccactgg gatttttacg
     1621 atcctttatc agctatcact tgcgatttaa ggtgactttt gcaggaaaga aagtcaaaga
     1681 acagaagaaa cgatgggatg aacccagtac tcttgtaagg agtgtgaact atgtatccga
     1741 ttaagtatat tgaaaataat ctcgtcttta accaagaagg cgagtgtttt gcctactatg
     1801 aattagtgcc ttacaattac tcctttttgt cacccgaaca gaaatttcaa gtgcatgata
     1861 actttcgcca actaattgcg caaaatcgtg aaggcaagat ccatgcgtta caaattgcca
     1921 cagaaagtag tattcgcgcc acacaagaac gatcaaaaaa agaaattacc ggtagactca
     1981 aagaagtagc gaaacaacga attgatatac agacagatgc attagtatcc atgattggcg
     2041 atagtcagat tgattatcgc ttttttattg gctttaaact aattgcgaca gatgaagaag
     2101 tcaatctaaa gagtctcaag aaatctttct tttctgggct tcaagaattt gtctatggag
     2161 tgaatcatca tttgatgggc gattttgttt ccttatccaa tgaagaaatt cgctgctatt
     2221 ctaagcttga aaaattaatg gaaagcaaat tggctcgaag atttaaagtc aggcgagtca
     2281 caccgagtga cctgacgtat ttgattgaac atatttatgg cgaaaaaggg ataccgtttg
     2341 aagaatacga atttcagcta ccaaagaaaa agctaaaatc agaaacactg gtgaaacgtt
     2401 atgacttatt acgtccaaat cgttgtttaa ttgaagaaaa accaaggtgc ttacggatgg
     2461 aacatgagaa tcatgagtcg tacgtagcgt atttaacaat taatacaatt gtgggggaga
     2521 tggaatttcc ttcctcagaa cttttctatt accagcaaca acaatttaca ttcccaatcg
     2581 acacaagtat gaatgtagag attgtgacga ataaaaaggc tttagctacc gttcgtaata
     2641 agaaaaaaga actgaaagat ttagacaatc atgcttacca atctgacaat gaaacgaatt
     2701 ccaatgtctt agatgctttg gattccgtgg atgaattgga aaccaccttg gatcagtcaa
     2761 aagaatctat gtacaaactg agttatgtgg ttcgagtgag tgccgaatca gtggatgaac
     2821 taaagcgacg ctgtgatgaa gtgctcgatt tttatgatga taccaatgta aaactagtcc
     2881 ggccctttgg cgatatgatg gggctacatg aagaattctt accctcaagt aaacgatata
     2941 tgaacgacta tattcagtat gttacgtctg attttttggc ggggcttggt tttggggcta
     3001 ctcagatgct aggagaatta gaagggattt actttggcta caatgtggat acaggtcgta
     3061 atgtttacct gaaaccggca ttggcttcac aaggagtcaa gggttcagta accaatgcgc
     3121 tggccgccgc tttcctgggt tctcttggtg gaggtaaatc ctttagtaac aatctcttag
     3181 tctactatgc tgttttgttc ggtggtcaag ctgtgattgt cgatccaaaa ggggagcgag
     3241 gagggtggaa agaaaccttg ccagaaattg cggatgaaat cagtatcctc aacttgacga
     3301 gtaagtcaga aaatcaaggt ctgcttgatc cttatgtgat tatgaagaaa aagaaagact
     3361 cggaaagttt agcaattgat attctgactt ttttaacagg aatttcaagt cgagacgggg
     3421 agaagtttcc tgtgttacgt aaagccattc gaacggtgac tcaaagtgat caacgggggt
     3481 tattacaagt tattaacgaa ttgcgaaaag acgggtcacc aatcgcggaa aatatcgccg
     3541 atcatatcga gtcctttacc gattatgatt ttgctcattt gcttttctca gacggctcga
     3601 ttacccaatc gattagttta gataaacagc taaatatcat tcaagtggcg gatttggttt
     3661 taccggatgc agaaacgact tttgaagaat ataccaccat ggaattgctt agtgtttcga
     3721 tgctaatcgt catttccact tttgccttgg actttatcca tagtaaccga gaaatcttca
     3781 aaattgtcga tttggatgag gcttggagtt ttcttcaagt ggctcaagga aaaacgttat
     3841 cgaataagct tgttcgtgct ggtcgggcca tgaatgctgg ggtctatttc gtcactcaaa
     3901 atagcgacga tttattagat gaacgtttaa aaaataatat cgggttgaag tttgcttttc
     3961 gcagtaccga tattcatgaa atcaagaaaa ccttggaatt ctttggtttg gatcaggagg
     4021 atcaaagcaa tcaaaaacgc ctgcgagatt tggaaaacg
//
LOCUS       DADJYG010000181         3988 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.187, whole genome shotgun sequence.
ACCESSION   DADJYG010000181 DADJYG010000000
VERSION     DADJYG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.187"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(11..553)
                     /locus_tag="JZ382_002476"
     CDS             complement(11..553)
                     /locus_tag="JZ382_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAY2428619.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            688..1077
                     /locus_tag="JZ382_002477"
     CDS             688..1077
                     /locus_tag="JZ382_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428620.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1081..1845
                     /locus_tag="JZ382_002478"
     CDS             1081..1845
                     /locus_tag="JZ382_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY2428621.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1974..2921
                     /locus_tag="JZ382_002479"
     CDS             1974..2921
                     /locus_tag="JZ382_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2428622.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3051..3791
                     /locus_tag="JZ382_002480"
     CDS             3051..3791
                     /locus_tag="JZ382_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428623.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1322 a          683 c          811 g         1172 t
ORIGIN      
        1 agtttgtgag ttattttcct tgtcgttttc tcactttttt agtccagaaa ccacctgaaa
       61 tatattttgg ctcataagaa atgataaaag cacgctcttc gatttctttg atcaggcgat
      121 acagttcacg ttcgcttttt ctaggagaaa gtatttcgag aactagccgc tcaccttcca
      181 gccccattgc agaagtctgt gtcacaccat aaccgtgttc tctcagtata cccggcaaac
      241 ttttatcttc tgcgctattt gctggcaaaa tcgttgtgac catgatgtat cctaatgcca
      301 atttgtcttc aatcaaaatt ccgacactga tacctactgc gtaacctaac gcatagacaa
      361 ataaattcag tggattatct aaacgatcta aaacaaggga taagccgacg atataaatcg
      421 tgatctcgac catactgaca agtggagcaa tcagtcgata gcctttcatt gtcaacatga
      481 aacgtaacgt gttcagagtg atataagcaa aattaatgag gaagatcatc cccaacattt
      541 ttggatcaat catttgtaaa actcctttca tttatataac aacaatatca aaaaaaatcg
      601 taagtgaaga aaaattgaaa ctttatacat acaaaaaaat gataaaatga ttataataga
      661 attataaaga taggttaggt gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc
      721 cacgtgctaa gtctccttct cagtatatac ttaggtgtct attcgattca acagccaatt
      781 gaggtagaga acattgtttt tcagatgtcg ctgacagatg gtttaatttt aagcgttttt
      841 ttctttttga tactgtttat tggtaatatt ttcggtagcg ttgccagttt tttgaatttt
      901 tctatttatc cactcctttc gctagcatta ggagtggtgg gactttgttc tctgtgctta
      961 tttcccgagc cattcagtcc tgcatttata ttatttggta cactgaacct gttccagaca
     1021 actgttggag catggttgct atggcgatca ggaaatttaa tgaaaatagg tgagtaacaa
     1081 ttgaatccaa ttatattgaa acgttctgtc catcatattc cagtgttaga agttgttctg
     1141 aaagaattaa gagaggaacg tattcccgtt gtcatctatt atcacggatg gcagacaagt
     1201 aaggagttag ttttgacgca aggaagaaaa attgcgcagc agggcattcg tgtgatcctg
     1261 ccagatgcga tgaatcatgg ggagcgcaaa cagccggttt cttctattcc atcctataca
     1321 ttttgggata gtatctacgg aaatctgttt gaatttgaca cacttatcaa gcatttagaa
     1381 aaaagaggtc ttctacatga tcagcttgca gttggcgggg tttcgatggg gggaatgaca
     1441 acatgcgcct tgttggctaa acatcctcat atagtagcgg gcgtgtgttt gatgggttcc
     1501 cctgcacctt tggaatatgg aaaagaaatc gtaaggcgcg catcggaata taattatgtc
     1561 gtaccaaaag actattttga tttgatttca tgggttaaga gctatgattt gtcattgcag
     1621 ccggaaaagt tagctggacg tcctctgttc ttttggcatg gacaagctga cgaaaaaatc
     1681 ccttatatac aaacggaacg atttgtagaa aaaaatcggc ataagtctta cggaaaagaa
     1741 attgtttttc gttcctcaaa aacacagcga catatggtac aaataccact aatggaagaa
     1801 gcttccactt ttttagcaaa aaatttgata aaaaagtata aataattgaa acggaaaatc
     1861 actcatgacg atagaggatt ttccgttttt tattttgggc tataataaac tctgttagga
     1921 aaatttggta taattgatag taagaatgac aacatgttag aaaggaagag agcatgccca
     1981 ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat gacggtctct cgagtcatca
     2041 atcatccgga acaggtgact gacgaattga aagagttagt tttctcagca atggctgaac
     2101 tggattaccg tccaaatatt gctgcaaaag cacttgtcac gaatcggtcg cagatcatca
     2161 aattatttat actggaagaa atcgacacaa ctgagcccta ttacatgaat ttgctgatgg
     2221 gaatcgctaa acggatcggt aaagcgcatt attctctcca attagttaca aatgatgcct
     2281 tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt
     2341 ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga aaattctgaa ggatatgatt
     2401 atgtagacag cgataatgaa tatggtacag caaaagcaac gcaatatgcc ttagaaagag
     2461 attatcaaac gattatttat atcggtatcg aagtggacga gccttttgaa atttcaaggg
     2521 aaactggtta tttatctgtg atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc
     2581 caaatcattc gacagaagta gaaagatata tgacagaaaa ctgggcagat tttacagata
     2641 aaacttgttt tgtatgtagt tcagatcggc tagcactagg agcagtaaga gggattataa
     2701 atgctggagg acacttgccc gaagaatttg gtgtgattgg atttgatggg gtatttttag
     2761 accaaatcag ttcacctaaa ttgacaacga tcaagcaaga tattatacgt atgggtgaag
     2821 tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc
     2881 gtttttttcc tgaactagtt atcagagaga caacgaatta agtcttatta ctatatatat
     2941 atttatgaaa ttcatcgcta ttttcttaaa ataatcagcg cattgctact gaaaacaatg
     3001 aatctgtgct attattgaga aaagttccat ttttgagaag gatggtttga atgaaagaaa
     3061 tcagacgaaa acatttccgt tttcctgcgt acgatgatga gttaggcgta aaattaacca
     3121 acgacaagaa gcgagtatta tttgacggac aagaagatct tttgactgaa cagaatcagt
     3181 ccccgtttga acagatgaaa gaatttcatt tcacgaacac gaacaccgag aaaatccgtc
     3241 gcaagcgtcg tgcaacaaat ggattgaagt cagctccgcg taaggaaaat ttggaaaagc
     3301 ataaaggaaa attaccggat tataccggcc attaccaatc agaaatcaca tcgacaggac
     3361 gaaaaaaact ttttggtgat tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt
     3421 ccaagcatac tacacgaaac gcatctgttg acacgaaggt gcgggagcgt tcttattttg
     3481 ttcctaaata tgtacccgca tcgattatcc cagaccctag agaaagcaaa atttctgagg
     3541 atcatttaat cgaatcgatg aaaaaaacgg atgactctta tctgttgttc gatacagaac
     3601 cgacgccgta tcaagaaaaa agaaatggtg agccgtctgt acgaacattc aatcgaacaa
     3661 aaacgttaga ggaaacaaag cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct
     3721 tagaacgctc attacaagga ttgatcgatg atcaaggaaa ctctttggga gaaaatagct
     3781 attttaaata agtgatatac aaatttatat tcggctacag ttggaaagga aacgctgcgg
     3841 cgtttcctta ttttatactc tatccgaaag tttatatcgt atcctcaaat ttttttgagt
     3901 ttaagcgaat tttttctccc aataagtaag aaaataatgg caaaataaaa aaacaaacta
     3961 ttttcgcaga tcgtttgcaa aaatagtt
//
LOCUS       DADJYG010000182         3984 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.188, whole genome shotgun sequence.
ACCESSION   DADJYG010000182 DADJYG010000000
VERSION     DADJYG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.188"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(93..1598)
                     /locus_tag="JZ382_002481"
     CDS             complement(93..1598)
                     /locus_tag="JZ382_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAY2428624.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1595..2281)
                     /locus_tag="JZ382_002482"
     CDS             complement(1595..2281)
                     /locus_tag="JZ382_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2428625.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2474..3895)
                     /gene="gndA"
                     /locus_tag="JZ382_002483"
     CDS             complement(2474..3895)
                     /gene="gndA"
                     /locus_tag="JZ382_002483"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY2428626.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1096 a          848 c          684 g         1356 t
ORIGIN      
        1 tttttataat gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt
       61 ggatatataa aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga
      121 ataaagattc taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta
      181 tagttttcaa ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta
      241 gctcttgctt tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt
      301 attccttcgc caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag
      361 atatggactt cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt
      421 aattgttcaa agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct
      481 aaagtgatca catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact
      541 tcttttcctt tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag
      601 tctagcatct cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat
      661 gattcttcta atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct
      721 tcaataattg caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt
      781 tgttcaatat acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc
      841 aattcatcat ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata
      901 gtatccattg tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa
      961 cctaaaatac tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc
     1021 ttatttcgaa tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt
     1081 tctcttgtac ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt
     1141 gtaacgatcg ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt
     1201 ttaaaaatca agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc
     1261 tcagctataa aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg
     1321 acctcatctc cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt
     1381 tcttcatccg agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt
     1441 ctttcttttg ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta
     1501 aacacaacga atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct
     1561 ttcagttctc ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc
     1621 cgcgtacagt ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac
     1681 gaatatatac gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta
     1741 gtaaaacgtc acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt
     1801 attctctttt tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt
     1861 cgatagtcaa atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa
     1921 tatcgattcg acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt
     1981 tgacgatgta gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc
     2041 ttgcagtcat cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta
     2101 aaccgttcaa ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg
     2161 ccgcatctaa accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca
     2221 actctagctc gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca
     2281 tttattattt tatcctttct attctactgg ctttttaaaa actctatcat tataccgata
     2341 atatgcttaa aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag
     2401 tctactcata acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac
     2461 agccgaaaga ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga
     2521 cacgctcata ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca
     2581 gacgttctgc tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta
     2641 cgccagcttg gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga
     2701 tttcaacgaa gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat
     2761 ccgtaatttt ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga
     2821 tttcaccaaa tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc
     2881 cttgggcata actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt
     2941 cttttttgtc tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta
     3001 cgcgttcttc tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa
     3061 gtggcacacc taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag
     3121 ctgcatcaag gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca
     3181 gaatgtcagc agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa
     3241 agatatcagc catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt
     3301 cagcaatcag ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc
     3361 cggctccatt tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa
     3421 tttgctctaa gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg
     3481 aaggtccttt taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc
     3541 cagagttggc taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt
     3601 caatcaaaat atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg
     3661 ccggtcctgc tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt
     3721 cttcgattgt atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg
     3781 ttttagcccc tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg
     3841 ctagattttt acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa
     3901 ctcctcctac atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat
     3961 tgcaaactaa agaacaacat attt
//
LOCUS       DADJYG010000183         3980 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.189, whole genome shotgun sequence.
ACCESSION   DADJYG010000183 DADJYG010000000
VERSION     DADJYG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.189"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            120..1046
                     /gene="cysK"
                     /locus_tag="JZ382_002484"
     CDS             120..1046
                     /gene="cysK"
                     /locus_tag="JZ382_002484"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY2428627.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1282..1728
                     /locus_tag="JZ382_002485"
     CDS             1282..1728
                     /locus_tag="JZ382_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAY2428628.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2001..3353
                     /locus_tag="JZ382_002486"
     CDS             2001..3353
                     /locus_tag="JZ382_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAY2428629.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3524..3811
                     /locus_tag="JZ382_002487"
     CDS             3524..3811
                     /locus_tag="JZ382_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428630.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1384 a          639 c          829 g         1128 t
ORIGIN      
        1 aattaccata tatggtatat ctttgtcttt tttttgctag tcaaagacga atcactcgcc
       61 ataaatgaac agattgtatt atgataagta agaagagcaa agacaaggag gaattgaaga
      121 tgacagctat ctataactca gtcaccgaat tgattggcaa aacgccaatc gtcaaattaa
      181 ataaaatcgt accagaagat tcagcagatg tttttgtaaa gcttgagttt tttaatccag
      241 gaggaagtgt aaaagatcgt attgctttaa gtatgataga aaaagcagaa cacgatggtc
      301 tactgaaacc aggtgatact atcattgaac ctacttctgg aaatacagga atcggattgt
      361 cgatggtagg agttgcaaaa ggatataaag taatcatcgt gatgcctgag acgatgagta
      421 tcgaaagacg tttattgatg aaagggtacg gcgcagagtt gattttgact cctggtgcgg
      481 atggtatttc tggatcaatc agagaagcag aacgtttggc aaaagaaaac ggctactttt
      541 taccattaca atttgaaaat gaagcaaatc ctcttgtgca tgagaagacg actggtcctg
      601 agatacatca agcatttgga gtaaatggct tggacgcgtt tgttgcaggt atcggtacag
      661 gaggaacgat cactggcgct ggccgagaat taaaacgtgt gtatccgaaa atcgaattaa
      721 tcggggtaga accagcagaa tctgctattt tagaagggaa agaagcggga cctcacaaaa
      781 tccaaggaat cggtacaggt tttgttccta aaacactgga tacttctgtg tatgataaag
      841 tcctttctat tagtggagac gaagcgatgg aaacagctag agaagttggg cgaaaagaag
      901 ggattttagt aggcatctca tctggcgctg caattgctgc cgcgctaaaa gtcgcaaaag
      961 aattaggaaa aggcaagaag gtattagctg tggttcccga caatggtgaa cgttatttat
     1021 ctacagccct ttaccaagag atataaggca cttggttttt ctccagatag gtttatgtaa
     1081 aaaactttta aagatgaaaa gaacacacaa aaatttatga tgagaaaaat tatttgaata
     1141 gagacgtaga atagtgaacg cgactgtttt acgtcttttt tcattaaaat acaataaaat
     1201 tgaattttct tttcatgtta agtggatttt aaaatgtaaa aatagtatga taagacgaga
     1261 ataacaacgt ggggtgttta catggaaaat aagttagtcg aagaggttat cgaaaagtta
     1321 aaaaaagaga acattcgaat cacacctcaa cggtacgcgg ttttagaata tttgattgaa
     1381 catcattccc atccaacggc tgatgagatt tataaagcac ttgaacatcg gtttccaaat
     1441 atgagtgttg cgactgttta caataacctt cgcttattta cttcgattgg atttgttcaa
     1501 gaaatgaagt acggcgatgc ttctagccga tttgatttca gttcaaagcg tcattatcat
     1561 gtaatttgtg aaaactgcgg taagattgtt gattttcatt acccagggct tgaagatata
     1621 gaaatggctg caagcagact gactgatttt gagattaatg aacatcggtt ggaattgtat
     1681 ggactctgtc cggattgtaa agagtcagca aaaaaggcgc aaatgtaaaa acagtttcta
     1741 caagtgttat acctaaaaaa agctagctgt gtaaaaaaaa cgctagcttt tttatttttt
     1801 tgtcttttat tcacaatctt agatataaaa gtgaatatta ttaaaataag aatcttgtag
     1861 atattgggtt cttatgcttg aaaaggaatg aaactacaaa caaacctttg tgaattcaca
     1921 agcattaaac tgttgtatcc gttttatttc tgttatagta gttttgtcga aggacaaaat
     1981 gttaatcgga gggaccataa atgaaagtag tagtaattgg ctgtactcat gcaggtactg
     2041 cagctgtaaa aagtatttta aaaaatcatc cagaagcaga agtgacagta tatgaaagaa
     2101 atgacaatat ttctttctta tcttgcggta tcgcacttta tgtcggcgga gtagtaaagg
     2161 acccagcagg cttgttctac tcaaatcctg aagaattatc ttcattagga gcaacagtta
     2221 aaatggaaca cgatgtaaca gacgtcgata cagaaaataa aacagtaacg gcaaaagatc
     2281 tgaaaacagg agaaacagaa acggtttctt atgataaatt agtgatgaca actggttcat
     2341 ggcctattat cccaccgatt agtggaatcg atgcgaaaaa tgtactccta tgcaaaaact
     2401 acaatcaagc aaatgaaatt attgcacaag cgaaagaagc aaaacgtgta gtaattgttg
     2461 gtggtggata tatcggtatt gaattagtag aagcttttgt cgaatctgga aaacaagtga
     2521 cattgattga tggattagat cgcatcttga ataaatatct agataaacca ttcacagata
     2581 tcttagaaaa agaattgaca gaccgcggtg ttactcttgc gctaggcgaa aatgttactg
     2641 aatttatcac agatgaagaa ggaaaagtga aacaagtagc tacaccaaca gatacatttg
     2701 atacagatat ggtcatttta tgtgtgggct tccgtccaaa tacaaaactg atcgaaggaa
     2761 aagtagatac actgccaaac ggggcaatca aagtgaatga atacatgcag acaagtaatc
     2821 cagacatttt tgcagctgga gactctgcag tagtcaacta caatccaagt ggcacgcaaa
     2881 actatattcc tttggcaaca aatgccgttc gtcaaggttt gcttgtggga aataacttga
     2941 cagatcataa aatggcgtat cgtggtactc aaggaacttc aggactttac ctatttggct
     3001 ggacgatcgg ttcgacaggt gtgaccaaag aaagcgcaac tttgaacgat ctagaagtcc
     3061 aagcgactgt atttgaagat aattatcgtc cagagttcat gccaactact gaaaaagtaa
     3121 tgatggagct agtctatgaa aaaggaacga atcgtatcgt aggtgctcag ttcatgtcca
     3181 aatacgacat tacacaatca gcaaatacca tgtctttagc tgttcaaaat aaaatgactg
     3241 tagaagatct cgcactttca gatttcttct tccaaccaca ttttgaccgc ccatggaatt
     3301 acttgaacct acttgcacaa gcagcgctag gcgaaatcga aactgcacaa taaagaaaaa
     3361 gggttgtgac acaattttgt cacgaccctc tgtctttttt tgtgaaaagt acaattagtt
     3421 gtctttcttc gaattttttg tcacaataag cagcaaagat aaagctgtta cataaaagaa
     3481 caagcagctt tatttgattt tgaaaagaaa ggtgagaaag aagatgaata aagaatattt
     3541 tgtccaacaa gatgcatttt tagaagatta taaaagtgct tcgaagaaat taacgatcaa
     3601 tattgatgag ttggcagatc aagtactctc tcagttgaat gaaacgcaag aggaatatta
     3661 cacgatgcca gcaaatgacg ccaaagataa tgtttctcat cgttttcctt tcagtagacg
     3721 catctactca gatgaaaatg acggcacatt gaatacagag tacacatatg aaggtcagcc
     3781 atatgtgcta gaaactgatg atgatcttta aagacagttt ttgaaaaggc cgctcatcct
     3841 taagtgatga caaaaaatca tcacaattac cgatgactgt ctctttacta tcttttaatc
     3901 agaaaaaggg gttatgacaa gctttgtcaa cccctttttt tcttggtttc agattgggtt
     3961 ttcctacaaa atatttttat
//
LOCUS       DADJYG010000184         3896 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.190, whole genome shotgun sequence.
ACCESSION   DADJYG010000184 DADJYG010000000
VERSION     DADJYG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.190"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..686
                     /locus_tag="JZ382_002488"
     CDS             <1..686
                     /locus_tag="JZ382_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAY2428631.1"
                     /translation="GNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLKIRVPF
                     DDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVVSQA
                     IEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEK
                     EIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVT
                     DYISGYLDK"
     gene            753..1247
                     /locus_tag="JZ382_002489"
     CDS             753..1247
                     /locus_tag="JZ382_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAY2428632.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            1303..1941
                     /locus_tag="JZ382_002490"
     CDS             1303..1941
                     /locus_tag="JZ382_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428633.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2013..2408
                     /locus_tag="JZ382_002491"
     CDS             2013..2408
                     /locus_tag="JZ382_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428634.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2392..>3896
                     /locus_tag="JZ382_002492"
     CDS             2392..>3896
                     /locus_tag="JZ382_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAY2428635.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA"
BASE COUNT         1382 a          588 c          746 g         1180 t
ORIGIN      
        1 gaggtaacga gattgtttct aaaatgaaaa tatcagaggt aactttagat gagactgacg
       61 attttactta ttataaagga tataaaaagg gctggaatac gctttgcaca tatcttaaaa
      121 taagagttcc ttttgatgat ttagactttt ttgaaagtca taaagatttg attacccaga
      181 cagcaagctc catatatgat aaacaaggtg ataatgtatt agtggatact attttagtac
      241 cacttccaga gaattacgaa gtaattaact ttagtcagtt gaaaattagt gatgtggttt
      301 cacaagctat tgaagacgct gagtctttta tgagtaatgg tgaatatcaa cgtgcctttg
      361 acagagttca cactgctttt catggatatt taattgaaat actaaaaaaa tatgaaatta
      421 cagttcctag agacgaaaat ctatctaaat tatatagtag gatacaacaa ttaattgaaa
      481 aagaaattca acctactgaa cttgctgata tagtgaagac aacgataagg tcatcaaatg
      541 gaatgattag ctctcttaat gaagcaagaa ataggcattc acttgcacac cctaatacaa
      601 atattattgg aaagagagaa gcaaaactaa taataggtat ttcaagtaca gttacagatt
      661 atattagcgg ataccttgat aagtagcgtc attaactgat tgctactttt tttatgacta
      721 tttttataaa aaggatgtga taagaattgg aaatgaaagt ctatgtggca aatttaggac
      781 ggtataatga aggtgaatta gtcggagcat ggtttactcc accaattgat gaggaagaaa
      841 tggcagaaag aatcggttta aatgaggact acgaagaata tgcaatacat gattttgagc
      901 ttccttttga tgtggacgaa tacacgccga ttagtgaaat caaccgtcta tgtgaagcca
      961 ttcaagaaat cgaaggtaca cctatttata atgaactcaa agaaattcaa ggcatgtggt
     1021 ttagtagctt ggaagaatta ctggaaaaca aagaggatat tcattgttat agtgattgtg
     1081 attcgatgga agatgtcgcc cgttattacg tggaagaaac aggacagctt ggtgaagtcc
     1141 cttccaattt acaaaattat attgactacc aatctttggg acgtgatatg gaaatagagg
     1201 gtaattacct agttacctct catggggtat ttgaatattg tcagtaagtt taacaatccg
     1261 tgttataatt aaattaataa atagcacgga gaggtataaa ttatggtaaa tgtatttgaa
     1321 aatcttaaac tattaaaact agacatgaaa gataaaggct gggtgataga ttccttttac
     1381 tttcgttaca aacagcaaaa ttatattgta cttgttaagc tatttgaaaa agaggaaaaa
     1441 gttccagaat atgctctgtt aaaactagaa tttttaaagg aaaacgattt ttcagatatg
     1501 ctagctgtct atgctaattc ggtaaaatta tatacagaca cgaaaacaat aagagaatat
     1561 tttggtatcg aatacagtag taacttggga gatgttttat ttcaatttag ccaaactctt
     1621 gctcgattta ttccgacaga agttagtgag aagaaaaatg aagaccaaaa agaagccatg
     1681 tgtttctcat taagtcaaag tgattcagaa gatcccagaa aaaaatattg tttttcagtt
     1741 agaagaaatc ctttaaaggg aaatggtgag ctgggaaaac gaagtccttt taatgacaat
     1801 aaaacgagat tgtatagacc cagcttatat gaacgattag ggtcagatac caatttaagt
     1861 ttcagatatt caatgaatcc agatgatgaa gaaactgatg aaggtattat tgcaaaatgg
     1921 acaaaaaata aaattgaata gttaaagcat tgaaagagca atcacctatt ttgattgttc
     1981 tttttctatt tttacggaaa ggacgtgaag acatgaaaaa aataagaagt tacaccagta
     2041 tttggtcagt agaaaaagtg atttatgcga ttaatgattt acaactccct ttcccaatta
     2101 cctttacgca aatgacatgg tttatcgttt ctttgtttgt cgtcatactc tttggcagtt
     2161 taccaccact ttctctgatt gacggagcac tactcaaata cttagggatt cccattgttc
     2221 tcactgggtt catgtgccaa aagacctttg acggaaagaa accctatgga tttctaaagt
     2281 ctatcattac ctatgctcta cgccctaaag taacctacgc tggcaaacaa atacgattaa
     2341 aaaaagaaac aatgaatgaa tcgattacga ttgtaaggag tgaacaacta gatggcatat
     2401 ccgattaaat atatggaaga taatctcgta tttaatcatg atggcgaagt attcgcttat
     2461 tatgaattaa ttccctataa ttacagtttt ttaagttccg atgaaaaaat tcaagttcat
     2521 gataatttct gtcaattgat tgcccaaaat cgtgatggca agattcatgc tctgcaacta
     2581 gcaaccgaat caagtattcg agccacacag gaacaatcga aaaaagaaat cactggacga
     2641 ttaaaagaga tagcttacga aaaaatagat gaacagaccg aagcgttaat ttctatgatt
     2701 ggtgacaatc aagtagacta tcgctttttt attggtttta aactctacct caatgaacag
     2761 gaagtatccg taaaaaatgt tgggaaagaa attcaaaaag catttgcgga attctttcat
     2821 ggtgtgaatc acaaattaat gggcgatttt gtttctatgc ctatggcgga aatcgaacgc
     2881 ttttctaaga tggaacagtt gttagaaagt aaattatcca gacgctttca gattcgtaaa
     2941 ttggagaaag atgattttgg ttatattatt gaacatcttt acgggcaaac aggtgtccct
     3001 tttgatgact acgactacca tttaccgaaa cgatacgaag ataaaaaagt gttggtaaag
     3061 aaatacgata ttctcaaacc cactcgctgt ttaatcgaag aaaatcaacg ctatttacgg
     3121 attgaacagg aagataccac tacttatgtg gcgtatttta caattaatag tattgttggt
     3181 gaattggatt tccctaatag tgaaattttt tattaccagc aacagcagtt tactttcccc
     3241 gttgatacca gtatgaatgt tgaaattgtc gccaataaaa aagccttatc taccgttcgc
     3301 aataaaaaga aagaactgaa agatttagat agccacgcat gggaaagcga caacgaaacc
     3361 agtactaatg tggtggacgc tttagaatct gtcgatgaat tagaaagtaa tttagaccaa
     3421 accaaagaat ctatgtacaa actaagctat gtggtacggg tatcagcaaa gaatttggac
     3481 gagctgaaac aacgttgtga tgaagtaaaa gatttttatg atgatctgtc tatcaaatta
     3541 gtacgacctt ttggcgatat gctaggcttg cataatgaat ttattccctc aagcaagcga
     3601 tatatcaatg actatattca atatgtcacg tcggattttt tggctggtct tggtttcggt
     3661 gctacacaaa tgattggtga aaattctggt atttacgtcg gttacaatct cgatacaggc
     3721 agaaatattt atctccaacc tagccttgca agtcaaggaa ttaaaggctc tgtaacaaat
     3781 gcattagcgt ccgcttttat tggttcatta ggtggcggaa aatcattctc taacaatatg
     3841 ctggtctatt attcagtact ctttggcgga caggcaacca ttgttgaccc aaaagc
//
LOCUS       DADJYG010000185         3874 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.191, whole genome shotgun sequence.
ACCESSION   DADJYG010000185 DADJYG010000000
VERSION     DADJYG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.191"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            299..613
                     /locus_tag="JZ382_002493"
     CDS             299..613
                     /locus_tag="JZ382_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428636.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            629..1015
                     /locus_tag="JZ382_002494"
     CDS             629..1015
                     /locus_tag="JZ382_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAY2428637.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1044..2429
                     /locus_tag="JZ382_002495"
     CDS             1044..2429
                     /locus_tag="JZ382_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY2428638.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2432..2584
                     /locus_tag="JZ382_002496"
     CDS             2432..2584
                     /locus_tag="JZ382_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428639.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            2607..3812
                     /locus_tag="JZ382_002497"
     CDS             2607..3812
                     /locus_tag="JZ382_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAY2428640.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         1278 a          707 c          833 g         1056 t
ORIGIN      
        1 ggacttatca cactttatca aggtcaaaac cactcaattt actactaatt tactacttat
       61 gaatgagctt tgatacgacg atttatcctt gaaaagtgaa gatataaaga tacttccaat
      121 aaaatttgaa tatttaatag gtagacactt caaaaaatga ggtgtctatt tttttacccg
      181 attttgaaag gaagtgaact tatgaaaaca aaaaatcaag aatcaaaagg tcgttcccca
      241 ctctttaaga ccatcaaaca ttcattcagc caataaaaaa gaaaggatag gtaaaaatat
      301 ggaacttaaa tttgtgattc ccaacatgga aaaaacattc ggcaatttag aatttgctgg
      361 cgaggataaa gtcgttcagc gaagaatcaa cggacggcta actgtcttat caagaagcta
      421 taatctctat tctgatgttc aaagagcaga tgatattgtg gtggtgcttc ctgctgaagc
      481 tggcgaaaaa catttcggct ttgaggaacg tgtgaagtta gtcaatccac gtattaccgc
      541 agagggctac aaaatcggca ctcgtggttt tacaaattac cttttacatg ctgacgacat
      601 gataaaagaa taaagaaaga gaggaaaaat gatgagatta gcaaatggca ttgtattaga
      661 taaagacacg acttttggag aattgaaatt ctctgctcta cgtcgtgaag tgagaatcca
      721 aaatgaagac gggtcggttt cagatgaaat caaggaacgt acctatgact taaaatccaa
      781 aggacaagga cgcatgattc aagtaagtat tcctgccagc gtgcctttga aagagtttga
      841 ttataacgca cgggtggaac ttatcaatcc cattgcggac accgttgcta ctgccaccta
      901 tcaaggagca gatgttgact ggtatatcaa ggcagacgat attgtgctga caaaggattc
      961 tagttcattc aaagctcaac cacaagcaaa gaaagaaccg acacaagaca aatagtcgct
     1021 aggtagaaag gagacttttt cgcatgaaac agcgtggtaa aaggattcgc ccatctggta
     1081 aagatttagt ctttcatttt acgatagcgt cactcctgcc tgttttcctg ctggttgtcg
     1141 gactgtttca tgtgaagaca atccagcaga tcaactggca ggattttaac ctatcacaag
     1201 cagataagat tgacattccc tatttaatta tcagtttcag tgtcgcaatt cttatctgct
     1261 tgctggtagc gtttgtattc aaacgggttc gctatgatac ggttaaacaa ctttaccacc
     1321 gtcaaaaact ggcaaagatg atacttgaaa acaagtggta tgaatctgaa caggtcaaaa
     1381 cagagggttt ctttaaagat agtgctggtc gtacaaagga aaagataacc tacttcccta
     1441 aaatgtatta tcgacttaaa aatggcttga tacagatacg ggtggaaatc acgctgggaa
     1501 aatatcaaga ccaactctta cacttggaaa agaaattaga gagtggcttg tactgtgagc
     1561 tgacggataa agagttaaag gattcctatg tggaatatac tttgctctat gacaccatag
     1621 ccagtcgtat ttctattgat gaagtagaag ctaaagatgg taaacttcgc ttaatgaaaa
     1681 acgtatggtg ggaatatgat aagctccctc atatgttgat tgctggtggt acaggtggcg
     1741 gtaaaactta ctttatactg acactgattg aagccttgct tcatacagat tcaaaactgt
     1801 atattcttga cccgaaaaat gctgaccttg cggacttagg ttctgtgatg gcaaatgtct
     1861 actatagaaa agaagacttg ctttcttgca ttgaaacatt ctatgaagaa atgatgaaac
     1921 gtagtgagga aatgaagcag atgaagaact ataagactgg caaaaattat gcttacttag
     1981 gtctcccggc acacttctta atctttgatg aatacgtcgc tttcatggaa atgctgggaa
     2041 caaaagaaaa caccgcagtt atgaataagc tgaaacagat tgtcatgtta ggtcgtcaag
     2101 ctggcttctt tctaatactg gcttgtcaac gtccagacgc aaaatattta ggcgacggaa
     2161 tccgtgatca gtttaatttc agagtggctt taggtcgtat gtctgaaatg ggctatggca
     2221 tgatgtttgg cagtgacgta caaaaggatt tcttcttaaa gcgaatcaaa ggtcgtggct
     2281 atgttgatgt aggaacaagt gtcatatcag agttttatac tccccttgta ccaaaaggat
     2341 atgatttctt ggaggaaatt aaaaagttat ccaacagcag acagtccacg caggcgacgt
     2401 gcgaagcgga agtcgcaggt gtggactgat cttgctggct ggtgtggcaa tagccacgcc
     2461 agcacttaac cccccgtatc taacaggggg gtacaaatcg acaggaaaca gtcaaaaaaa
     2521 cattagaaaa tcctttggtt acaagggatt tacaaaattt cagcgtatgt caaatgggct
     2581 ttaaaagttg acatacgcct ttttgattgg agggattttt actgaatgaa caaacttggt
     2641 tacagcattt aaaagaaaaa cgcttggctt atggactatc tcaaaaccgt ttagctgttg
     2701 cgactggtat tacaaggcag tatctaagcg atattgaaac aggaaaagtc aagccatcag
     2761 aggatttaca gcagtccctt tgggaagctc tggaacgctt caatcccgac gctccccttg
     2821 aaatgctgtt tgattatgta aggattcgct ttccgacaac agacgtacag caggtggtcg
     2881 aaaacatctt acaactgaaa ctgtcctatt ttcttcatga ggactatggt ttctattctt
     2941 attcagagca ttatgcttta ggcgacatat tcgtcctttg ctcccatgaa ctggacaaag
     3001 gagttctggt ggaattgaaa ggtcgtgggt gcagacaatt tgaaagctat cttctggcac
     3061 aacaaagaag ctggtatgag ttctttatgg acgttttggt ggctggcggt gtgatgaaac
     3121 gccttgacct tgccattaac gataagacag ggattttgaa tatccctgta ctcactgaaa
     3181 agtgccaaca ggaagaatgt atctccgtct tccgcagttt taaaagctat cgcagtggcg
     3241 aactggtacg caaagaggaa aaggaatgta tgggaaacac cctctatatc ggttcattac
     3301 aaagtgaagt ttatttctgt atctatgaaa aggactacga gcagtacaag aaaaatgata
     3361 ttcccattga agacgcagaa gtaaaaaacc gttttgagat tcgattgaaa aatgagcgtg
     3421 cctattatgc agtccgtgat ttactcgtct atgacaatcc agagcatacc gcctttaaaa
     3481 ttatcaatcg gtatatccgt tttgtagata aagacgattc caaacctcgt tctgattgga
     3541 aactgaatga agaatgggct tggtttattg ggaacaatcg tgaacgatta aaactaacca
     3601 caaaaccaga gccttactcc ttccaaagga cgctgaactg gctatctcat caagttgccc
     3661 cgaccttaaa ggttgcgatt aaacttgatg aaatcaacca gacgcaggtt gtaaaagaca
     3721 ttctcgacca tgcgaaactg acagaccgac acaagcagat tttgaagcaa cagtcagtaa
     3781 aagaacagga cgtgataaca acaaaaaaat aactcaaata caaattcatt gaatatagag
     3841 aggagaacat ttttatgaat tttggacaaa acct
//
LOCUS       DADJYG010000186         3864 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.192, whole genome shotgun sequence.
ACCESSION   DADJYG010000186 DADJYG010000000
VERSION     DADJYG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.192"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..135
                     /locus_tag="JZ382_002498"
     CDS             <1..135
                     /locus_tag="JZ382_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2428641.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="JZ382_002499"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="JZ382_002499"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY2428642.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="JZ382_002500"
     CDS             complement(2412..2663)
                     /locus_tag="JZ382_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAY2428643.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="JZ382_002501"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="JZ382_002501"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAY2428644.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="JZ382_002502"
     CDS             3613..3831
                     /locus_tag="JZ382_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428645.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1080 a          827 c          707 g         1250 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caag
//
LOCUS       DADJYG010000187         3809 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.193, whole genome shotgun sequence.
ACCESSION   DADJYG010000187 DADJYG010000000
VERSION     DADJYG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.193"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..258
                     /locus_tag="JZ382_002503"
     CDS             <1..258
                     /locus_tag="JZ382_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAY2428646.1"
                     /translation="NGWKVKLQERFELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKV
                     VDVKGMQTKDFKIKAKLFCSQYRVPLILAKKYRNTFKEERF"
     gene            273..434
                     /locus_tag="JZ382_002504"
                     /pseudo
     CDS             273..434
                     /locus_tag="JZ382_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            431..736
                     /locus_tag="JZ382_002505"
     CDS             431..736
                     /locus_tag="JZ382_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428647.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            736..1047
                     /locus_tag="JZ382_002506"
     CDS             736..1047
                     /locus_tag="JZ382_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428648.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            1214..1630
                     /locus_tag="JZ382_002507"
     CDS             1214..1630
                     /locus_tag="JZ382_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAY2428649.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            1630..2298
                     /locus_tag="JZ382_002508"
     CDS             1630..2298
                     /locus_tag="JZ382_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAY2428650.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            2295..2672
                     /locus_tag="JZ382_002509"
     CDS             2295..2672
                     /locus_tag="JZ382_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428651.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            2669..2968
                     /locus_tag="JZ382_002510"
     CDS             2669..2968
                     /locus_tag="JZ382_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428652.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            3210..3626
                     /locus_tag="JZ382_002511"
     CDS             3210..3626
                     /locus_tag="JZ382_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAY2428653.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         1371 a          566 c          930 g          942 t
ORIGIN      
        1 aacggctgga aagtaaaact tcaggaaaga tttgagctga tgccgaaatt tgaactagac
       61 ggaaagaagt atcgcaagat cgagtatatt cccgacttca cattttataa aaacggcaaa
      121 ctagtcaaag tcgtagatgt taaagggatg cagacaaaag atttcaaaat caaagcgaaa
      181 ctcttctgca gtcaatatcg agtaccactg atcttagcga agaaatatcg gaatacgttc
      241 aaggaagaac ggttttaacg aggtgatcca tcatgacaac agaagaagtg attcaaatgc
      301 gtattcgaag ccttcagcgt gagattgacg atctgtaacg gacaaaggca gtgatggtca
      361 atgaaacggc gaggaaggca atcgatttgc acatagaaaa tttaagaagg aaaatccatc
      421 gattggagga atgagcgtgg ataagaaagc agcaatgaaa cgaatcatcg aactgacaca
      481 ttctgagaat tggcaagaag acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc
      541 aatgtgggct gaaaagccta aacggaaaac gccgagaaaa attgcaatct ggcatggtga
      601 tcgaattcta gtaacaggta ctgctgaaca gttatctgaa attactggat taagcaaaaa
      661 cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac agtttaggta
      721 tgtggaggag aaataatgaa cgaactaatc acaaaagtag agcagtgggc taaagacaag
      781 ggactggatc aagcggatcc aaaagcacaa tttttgaaag tagctgagga attcggagaa
      841 attgcatcgg caatggcaag aagtaatgat gagctattta aagatagcgt aggagacgtt
      901 atcgtcacgc tgattattct ttccatgcaa aaaggggcaa acatacaaga gtgtttagaa
      961 atggcgtata acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt attcgtgaag
     1021 tcgagtgatt tggaggacag cgaatgatga tgccaaaaga aatctattca gatgcattaa
     1081 gaggtgctag aaatcaattg aaaatggcaa aaagagcata cgaaattcgg cccacaatcg
     1141 aaaatgaacg cagagtaaaa gcaattcgcc gtagatgttc aatttacggc gagttacaga
     1201 aggaggacag caaatgatac cgagatttcg agcttgggat aaacgaaaga acgtaatgag
     1261 agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat
     1321 aaatcctacc gaattgaaat catatcacgt gcgaaacatc gagctcatgc aatcaacagg
     1381 gctgaaagat aagaatggca ttgagatttt cgagtgggat attgtatcag tcagcgtgcg
     1441 aaatggattc gattatttag ataataaagt ttgtgttgtc aaaaattcaa taggacattc
     1501 gggattagtg tgtgccactg ttgatgaaga tttagagtat caaattttta acacagagct
     1561 gtttgaagaa tacacgtatg aagtcatcgg aaatatttgg gagaatagcg agttattgga
     1621 ggaacagtga tgaataaaca ggaattgatt gatgaattag ctaaatatgt aaagcgttat
     1681 gagaacgtta tggatgagca tggccaagga aggtacggcg cttatgaagt atctttaaag
     1741 ttggtgaaaa gactaaatga atcaaaaatt acggacgaac aagcttggaa taaggtagct
     1801 gaggcttatc ctgaatcggc acaaagcttg agaaacactt tagataatgc tgtatttggt
     1861 aagactggtg aaccgcagaa gcacgttatg ccgaagtttg tggcggagtg gatagaatac
     1921 gctaaaaaga aaggtgatag tctagctatt tcattcaagc cgtggaacct ctatggtgtt
     1981 gagtatagca aggctgatag atggattgga gataatcaag aaacgtttgc tcgtgcttgg
     2041 ctagacggct acgaggtcga gaaagagccg ttgtattatg tcccgctacc atatgaagtt
     2101 tgggatgaag aggcagcgga gcttaaaaca gagtatttgt atctacatta tgagatcaca
     2161 agtgatgaaa ctagaatatt tcctacaaaa gaacctagaa aaggttttgt cgcaaaatta
     2221 gatgaactta cgattaaatc agcagatgaa aactactggc cgtttgctgt accagtggaa
     2281 gaggtggtag aaggatgaaa aaattgcacc aactcatatc tgagaaagaa agcgaactcc
     2341 aaaatttaga agatagtttg ggattggggt ttccaattgt tgagcaagtt aaaatggttc
     2401 aaatcagtca tctacaactt gaattggaag atttaagaca aatagaagat ccgtatcaac
     2461 tcaacgataa ccagcaaatc gtgttggagt ggttgaaact aactgcgtca acaggaaagc
     2521 caatgcaagt tgtattttgg atgatgaata atgcagcatg ggggcattta gacgaattga
     2581 gggatccttt gatggaatta actgacaaac agcaattcga agtcctaaca gcattcgctc
     2641 aatggggatt agaacaggag gaaaaagaat gaaactaaaa gacggatttt acgctagtag
     2701 ccacggtatc ggcggtttaa tgctagacat gccgacaaag aaccctaaaa cacgtaagaa
     2761 gccaaaagtc aaagtcggtg acatggttcg ctgtgaagca gaaggattca tctatccatt
     2821 tcgtggatat gtagaacacg tctataatca ctcagcgatc attcgcattg aaaacacgat
     2881 ggaatgcgac aagttattag cgaaaagcaa agataattta gcagtggtga gattggtgga
     2941 tatggaggtt ataaacaatg aaatttaaaa tctttgaaga ggacactcgc tataaattag
     3001 aaaaaaaatt aaatgaattt gcaaagaata atgaaattca gcatatatct ttaacggctt
     3061 ctaagtcagg ttatacaact tactatgcag ctgttgtgag ctacgtaatt caataaaagg
     3121 aactcggcaa ataaaaaagc cggatcactc cgactgatgt aataaatccg acaagtttat
     3181 tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta gatttcaaac
     3241 agacaagatg taatgcgaga gatgtgctga agaactttcg gcgcttggag cggatggcag
     3301 gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag gcaccgaagc
     3361 acggcaataa ggcagaagac gcgatcattc agatgatgga tatagaagcg gagagagacg
     3421 cgattttagc ggctttgatg gcgcttagtc tgattagccg tcagatactc tactacagct
     3481 tctgtgacgt aaacaagcac tctaattatg aaatagggca attgatacga ggatacggag
     3541 agaagaatgt agagaagctg aaatccatcg cattgatcga atttgctgaa gcatacaaaa
     3601 aaggcgtgtt agttcagtat cgttaatttt gtagggtttt tgtagggata gtgtagggtt
     3661 tttgagcggt ttaacgtgat attatgatag tgtcgaaaga ttagtgatag gtctaagaca
     3721 aaataataat aaaaggaaca tcgttttatt attgtttcac aattaagctt cgatagacag
     3781 cagcggaaat attaagaata aggatgtga
//
LOCUS       DADJYG010000188         3774 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.194, whole genome shotgun sequence.
ACCESSION   DADJYG010000188 DADJYG010000000
VERSION     DADJYG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.194"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            492..605
                     /locus_tag="JZ382_002512"
     CDS             492..605
                     /locus_tag="JZ382_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HAY2428654.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(693..986)
                     /locus_tag="JZ382_002513"
     CDS             complement(693..986)
                     /locus_tag="JZ382_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAY2428655.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1389..2567)
                     /locus_tag="JZ382_002514"
     CDS             complement(1389..2567)
                     /locus_tag="JZ382_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428656.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2671..2985)
                     /locus_tag="JZ382_002515"
     CDS             complement(2671..2985)
                     /locus_tag="JZ382_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428657.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(3305..3700)
                     /locus_tag="JZ382_002516"
                     /pseudo
     CDS             complement(3305..3700)
                     /locus_tag="JZ382_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1207 a          721 c          553 g         1293 t
ORIGIN      
        1 tatttttaaa taaaaaatac tcttttttta cgattattga aaaaaaaaga taaaacttga
       61 ttaattgaag tattgcttaa atttggaagc tatgagggaa gaataaaaga gttaggacgg
      121 gattatctac tatttttctt tgttttaaat atttgttatc tttgaacata aaagagaaat
      181 gtgtcgactg cagtgttgtg tccctcatca cgaataaacg gtgttcaatt agctgatctt
      241 gtattcactt aaaaaatggt aaagtacgag ttgctattcc ccattttcaa ttttctaacg
      301 ctcaggacaa aatactgttt tcacaacctc attggaataa aagcatatat attgtttcaa
      361 gaacgaaatc ttgttatctt tttaaagggc atctgtccaa aatctaattt taggaggtac
      421 gttttggtat ttattcgtac aaataaaaga gaattatctt tattcatgta acgcatccaa
      481 ttgatttttt catgtacgct cctggaacga ttttactgac gattgcttat ttaactgcga
      541 atcgctcatt agcttttatc atagctatcc atattttgaa taacgttctt ggctttgtat
      601 tatagataca gatgacaaaa aaagttcaag tatgcggtgt agaaccaata tcgcatactt
      661 gaactttttt caatgagtat aaattaaaac ttctaatttt caattcccat catctgatat
      721 gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga ttagataacg tctttgttcg
      781 acataatcgc cactagaaaa tacgttcagc agtatcgaat taacctctgt ttctgtataa
      841 gtcactccat ggtcaaagcg tttcaagact tctttcaata ggatgtattt cttttttgtt
      901 gaacttggta cttgttttac ttttccatca ttcatgaatg tgtctaaaac tttcatcttt
      961 tcttgctccg tataagtata aaccatcttc tttctccttt tgattctaag aatactccat
     1021 ttatgttaga atattctgga ttttttatta aagcggttac tcttaagaaa gatattatca
     1081 tagtgctctt ttttttacta ctacttatag gtagatttat tctaatccat tattctcccc
     1141 taccaataat taaagaaaaa actggatcag ggtgttcaaa ttacatgcta agaaaaaaga
     1201 acccttgaat accaagggtt ctttctcagt ctgataacaa tgattaacga cggatttctt
     1261 tgatacttgt gaaaagtcgt taaagtattg atataaaagg atttttatta cctctgacta
     1321 catattgact aatttgttaa aaaatcgttt ttcaagaatc ctgtaatacc agtgctttca
     1381 ccgttttttt aactctctaa agaactaaaa gcaatcagag gttttacctt ttcatttgac
     1441 gttaagtggc gataaatttt cgaagttgtt tggtcagtat ggcctaagcg atctttcaag
     1501 acttcccagc tacagtgttc cgctactaag cttgccattg tatgacgagc taaatgagta
     1561 tgaaatggct tattaattcc tgagcgattc acgcaccgtt tgacgtgatc atttacatac
     1621 tctcgacgat aaggaacacc acgtttacta gaatcaagat acacaaacaa atatgtttct
     1681 tctataataa acctgtaatg ttgcctcatc tcacccaaag ccttaatcaa ctgttgtagc
     1741 ttttccataa ctagaggagt tactagaact cgtctagcag aactaactgt cttagtagtt
     1801 tcaattcgaa aatccttact cttaccttta actgccgttt gcttatagaa cagaatttca
     1861 tttcttttga aatctatatc tccaggctgt agagcaagcg cctcgctgac tcggcagcca
     1921 gtgtaaaata gaacatggaa aagtacagac atcggtaatt cctcatgaac aatgccataa
     1981 ttcaaaaaag ttctcaattc atcgacagta agaaacttgt cctcaatatt atttatggct
     2041 tcttttaaat cagttaccgt taactgatat tttggcattc tcacagactt cattggattt
     2101 tcacttatca aattctttct ttgacaatat ttaaaaatca tatttagaac ttgaataagt
     2161 gattgtaaac tatttttggc atagccaaca tttgttccat taacgccaca ttcctttaag
     2221 gtaaaaagca attcttgaag gcactctggt gtcaaatcag acaacttttt atcaccaatc
     2281 catcgaacaa taatttttaa ttgttcctct cgcttatcat aggttatttc cttttgttca
     2341 tttaacgaac ggtaatattg gaaccattgc tcacctacaa cactaaactt gtcattgctt
     2401 gcaacaattt ttttcttaag cctttgttgt ttctccattt ccagataagc gagttgcgcc
     2461 tcacgttttg aattaaagcc acgtcttgtt gttcgatatg cttgaaagta ccatgtgatc
     2521 ctaccagtag aactgatttg cttcttgaat cgatctgttt tttccattta ttgattcctc
     2581 ctattttgaa ggaacccaac aagcagaaac cctttggaaa tctttctatc tatgtcatca
     2641 ttgtaccgca tcttagaaag aaaatgtaac ttataaagat cgaagtagtc gcaatttttc
     2701 tcgatagtca ttcgaatcac tctttctcat ccattctaaa acatcatcac gaaaatagtg
     2761 tcctcgaatc ccgtaccaac agggaggaaa ctcaccaagt tttactaaac ggttcagtgt
     2821 actatcactt atctgaaata gtcgctttaa atcagtattt gttaatacca gaggtaaatg
     2881 actgactgca agcaattttt ccgtgtaatc ttccagtttt ttttgtaaaa ttgaatagaa
     2941 ctctatttcc agagaattaa catttgtttt aacttccaac tccatcaccc ttctctctca
     3001 aaatcaaaac gaccaccacg gtacccctta aaattgtaaa aaaacactcc agtactccat
     3061 caaaacggac tagaaaagag gaatttttaa tatgaaacaa ggaattgcag acataaaaat
     3121 aattaaagaa attcttgaaa aatctacggc taatgcaata gcattcggaa caggaatcaa
     3181 cttgagtaca gtaaaaaaac ttaaaagtgg agagagagct gaagaaaaat taaatcttgc
     3241 cgatgctatt aaaatcacag aatttggcat gaaaaatatg ccaaccaaaa tcgaaatatg
     3301 gaaatcctag ctcttccaaa gccatttgtc gttggtacca aacactgccg agacttttcc
     3361 caataatatc gccaatttca cgatcattaa gaccgatgaa gaaggacata aaaatgattt
     3421 cttgtctttg actaggtaat gctgcaatcg catttgccaa ctcctcattg gaaataaaga
     3481 ttttcatacc aaggacaata aaagtaccac tactattcaa atttggttcc aaataataga
     3541 actctgtacc taaccctttt tgttccaata tttccattga tatttgccgt tggcgaaatc
     3601 tagcgtactt cttttgaata tttaacgcct cattgcagac tactttctta caaaaactgt
     3661 caaacgtatg ttgaatgtgg gtttcctctg agtcgcccat aatattctcc ccctcaaaaa
     3721 attcattcaa ctcgttttac ttctatccct ttccattatt actacaatct agga
//
LOCUS       DADJYG010000189         3752 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.195, whole genome shotgun sequence.
ACCESSION   DADJYG010000189 DADJYG010000000
VERSION     DADJYG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.195"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(573..1130)
                     /locus_tag="JZ382_002517"
     CDS             complement(573..1130)
                     /locus_tag="JZ382_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428658.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="JZ382_002518"
     CDS             complement(1309..1920)
                     /locus_tag="JZ382_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428659.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="JZ382_002519"
     CDS             complement(2033..2290)
                     /locus_tag="JZ382_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428660.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="JZ382_002520"
     CDS             complement(2887..3165)
                     /locus_tag="JZ382_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428661.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="JZ382_002521"
     CDS             complement(3199..3450)
                     /locus_tag="JZ382_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428662.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1130 a          767 c          508 g         1347 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta at
//
LOCUS       DADJYG010000190         3745 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.196, whole genome shotgun sequence.
ACCESSION   DADJYG010000190 DADJYG010000000
VERSION     DADJYG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.196"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            641..1969
                     /locus_tag="JZ382_002522"
     CDS             641..1969
                     /locus_tag="JZ382_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY2428663.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2171..3004
                     /locus_tag="JZ382_002523"
     CDS             2171..3004
                     /locus_tag="JZ382_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY2428664.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3020..>3745
                     /locus_tag="JZ382_002524"
     CDS             3020..>3745
                     /locus_tag="JZ382_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAY2428665.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIW"
BASE COUNT         1241 a          592 c          782 g         1130 t
ORIGIN      
        1 gactgttaat catgatgtcg taggttcgag tcctactgcc ggagtaaata aagcttatgc
       61 aaagaaaaag aaatccaatc acttgggttt ctttttcttt tattattatg atgaatcaag
      121 ctttctgttt ttgtcacgta tgttgtaatc tttacgaaag agtagagttg gctactcata
      181 atcaaccata cttcccatca tcgctaaata cgaaataaat cagcattcct acaccaatca
      241 aagcacaagc tgcaccaaga aaagggatga cattcccaaa gttatctgta ttcataagta
      301 tgccaccacc cacaaacaaa aagccgccag caatcaggtt tttcatttta ttcatctcct
      361 ttcttacgtt atagtatttc cttctttcaa tgagtatttt gaaagctatc ttattgtatg
      421 atgttcttct aggcctcttc tttattatac aggccatttt cgttatctca tagaaatatt
      481 cctttttatc tgaaaactat tgtataccgt atgacatttt tcttcatgaa gttgtaagta
      541 tattctcatt caaatgaaaa tttcatcata gaagagattg tatttctaaa ttcttaagag
      601 taaaatacga agtataactt ggatttggaa ggatgattgg atgaaagcta ttacgcctga
      661 tatgacaaca aaatttaaca aacagtttca agcaaatatc aaacagcagg cattgcagcg
      721 ggcagttgtg aagaacggaa ttactgcttc tgctgaaaat gtttcagcac atgtggagaa
      781 tacaccggta ttttctatag atttggctac ggggaaagtg gctaatcaaa tgcaatcggg
      841 gcgttgctgg atgtttgctg cactgaacac attccgtcat aagatgctga atcaattcca
      901 tttaaaagag tttgaattat cacaaaatta cacttttttc tgggataaat atgaaaaggc
      961 aaattatttc tatgaaaaca tcattgcaac aggaaaagag gcaatcgata gccggaaagt
     1021 agctttctta ctcgctactc ctcaacaaga tggcggccaa tgggatatga tcgtttcatt
     1081 attccaaaaa tatggcgttg tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa
     1141 ttctcgagat ttgaataact acttgaacaa attactgcgt aaagatgcag tagtgttacg
     1201 gaaaatggtt gctcaaggag aaacattaga cgctattgaa gaaaagaaag aagaaatgct
     1261 agaaagcatt tataacttct tagctatctc attaggaaca cctccaaaag aattcgattt
     1321 tgagtaccgc gatgaagaaa agaactatca tcttgaccga ggactgacac cacaaatttt
     1381 ttatgataaa tacatcggtg taaaattaga tgattatgtt agcgtgatca atgcaccaac
     1441 aaaagacaaa ccattcaatc gttcttatac agtagagatg ctaggaaatg tcgttggcgg
     1501 gaaagaagtc aaatatctga atgttgatat ggaaactttc aaaaaattag cgattgctca
     1561 gcttgaagaa ggagaatctg tttggtttgg ttgtgatgta ggtcaatctt ctactagaac
     1621 tagtggcatc atggccttag atgcttattc aatggatgat ctatttgata cggattttac
     1681 aatgactaaa gctgaacgtt tagattttgg agaaagtctg atgacccatg cgatggtatt
     1741 gaccggtgtt gacttagtag acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg
     1801 cgaaaaagtt ggaaagaatg gcttctttgt tatgagtgat gcctggatgg acgaatatac
     1861 gtaccaaatc gttgtaagaa aagatttatt gacgaaagag caattaaatg cgttcaatga
     1921 agaaccaatt gttctatctc cgtgggatcc aatgggtgct ttagcataga cgagtatttc
     1981 aatgaagagt atgtagtaaa gataaaaaga gctggcttaa gccagctctt tttatcttta
     2041 aagtccattc cctgtttttt atggtccctg tgctataatt ctaaataaag gataaggatt
     2101 ggaaataatc cttttatcat taaaaatatg atgacgaagt agaataaaat tgggagtagg
     2161 ggggaaagtc atggatatcc aaactgtttt atctgatctg aaattaaatg gtaaagtgat
     2221 tccagttgtt ggtggcgatg ttaatcagac ctatcgtata aaaaccgaac atcgtgctta
     2281 ttttttaaaa atccatccga atgtaaaaaa aggatttttt gaagcagaag tagacggatt
     2341 aaaagaactg tcagcattcg tacgtgtacc ggatacgtat atgctaggtg aaacaagtga
     2401 aggcgcttat ttgttaatgg aatggatcga gccgggaaaa ggggatcaac gagatcttgc
     2461 tgcagcttta gctaatctcc atcagcaaac agcgccacaa tttggttttc gtaaagataa
     2521 ctatttgggt acacttgttc agaaaaattc ttttgaagaa gattggtgga cttttttctt
     2581 taaagatcgg cttgaatccc agatttcttt ggcagaagaa acgaatcgat ggaatgtaca
     2641 gcgtcaagaa aagtatctgc gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa
     2701 gatcactcca cgcctcttgc acggagacct gtggagtggt aatgtgttct ttgaccagca
     2761 agggcatcca gtttttgtcg acccagctgt ttcttatggg aatcgggaac aagatattgc
     2821 aatgagccag ttattcggtg gtttccgacc ggaattttta gatgcttatc aaactatatt
     2881 tccattagaa aagggatgga aagaccgact gccgatttac cagttatatt atcttttggc
     2941 acatttgaat atgtttggag aaagttacgg ttcccaagta gatcaattac tagaaaattt
     3001 ttaagaggag ggcgtgtttt tggcaaaaaa atataccaaa aagcatgaag tttcatatta
     3061 tgagtgtgac atcaaccaaa cgatgacatt tccttcgatg ttaggagtag ttatcaaaac
     3121 gtctgaagat caaagcgacc agttgggaag aggcagtgaa ttcgtcaata gctttggact
     3181 tacttgggtc atcacgaatt attccatgga gattaatcgt ctccctagag tcggtgaagt
     3241 aatctcagta acgactcaag ccatggagta caataagttt ttctgttatc ggaacttctg
     3301 gatccatgat gaagatggga aagaattagt caaaatagaa tctgttttcg tattgatgga
     3361 ttttgtgaat cgaaaaatga gcagtgttaa tgaagagatc attgctcctt ttgagagtga
     3421 gaagatcaag aaaatcaagc gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt
     3481 gccatatcgt gttcgttttt atgatatcga tagtaatcag catgttaata atgcgatgta
     3541 ttttaattgg atcattgacg tattaggata tgactttttg actacgcatg ttccagaaaa
     3601 agtaattatt cgatttgata aagaagtaga gtacgggaat gagatcgaaa gccattttga
     3661 acaaatccct atcgaaaatg gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct
     3721 gtattgtgaa gcaaatatta tatgg
//
LOCUS       DADJYG010000191         3718 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.197, whole genome shotgun sequence.
ACCESSION   DADJYG010000191 DADJYG010000000
VERSION     DADJYG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.197"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            102..2543
                     /locus_tag="JZ382_002525"
     CDS             102..2543
                     /locus_tag="JZ382_002525"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAY2428666.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="JZ382_002526"
     CDS             complement(2873..3577)
                     /locus_tag="JZ382_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428667.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1058 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DADJYG010000192         3700 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.198, whole genome shotgun sequence.
ACCESSION   DADJYG010000192 DADJYG010000000
VERSION     DADJYG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.198"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(4..915)
                     /locus_tag="JZ382_002527"
     CDS             complement(4..915)
                     /locus_tag="JZ382_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY2428668.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1372..1800)
                     /locus_tag="JZ382_002528"
     CDS             complement(1372..1800)
                     /locus_tag="JZ382_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428669.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(1828..2466)
                     /locus_tag="JZ382_002529"
     CDS             complement(1828..2466)
                     /locus_tag="JZ382_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428670.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2470..3240)
                     /locus_tag="JZ382_002530"
     CDS             complement(2470..3240)
                     /locus_tag="JZ382_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAY2428671.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
BASE COUNT         1106 a          707 c          555 g         1332 t
ORIGIN      
        1 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
       61 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
      121 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
      181 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
      241 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
      301 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
      361 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
      421 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
      481 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
      541 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
      601 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
      661 ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc
      721 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
      781 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
      841 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
      901 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
      961 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     1021 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     1081 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     1141 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc
     1201 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
     1261 attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt
     1321 taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata cttaatacca
     1381 accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg cacttgcata
     1441 ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt tcgcttttgc
     1501 accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc tcgtacaagt
     1561 tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc tagaaatttc
     1621 ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat gaatctctcc
     1681 tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca cttcagatga
     1741 catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg cttttttcat
     1801 aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc ctattttggt
     1861 cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt gttgcgataa
     1921 gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt catcggctaa
     1981 aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc
     2041 agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc
     2101 atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa
     2161 attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagatatggt
     2221 atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt gatcgaacaa
     2281 atagaattgt cctgaactga actgatcatt caatcctaat atattcaata acgtactttt
     2341 acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag tgaaacgatc
     2401 caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg
     2461 ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat
     2521 aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt
     2581 aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt
     2641 catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc
     2701 gattttccga caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa
     2761 agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt
     2821 gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg
     2881 actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa
     2941 cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt ttttcaatgt
     3001 cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa atgtataaat
     3061 gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt cttgataata
     3121 ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt cagggatcaa
     3181 tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga catcaatcaa
     3241 aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact gttcattttc
     3301 ttcattcaag taatctgata cattgactaa tatacttccg tttttattca aagtttcata
     3361 taattcctga tctgctgctg atgcaaaacg attggaataa ataaaatcca cgtggttttt
     3421 cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt ttggtggttc
     3481 atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga cattggcaac
     3541 ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga tatagttgaa
     3601 cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag agataccata
     3661 tattccgctg aacaagaaaa cgtacatctg gaacaaataa
//
LOCUS       DADJYG010000193         3659 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.199, whole genome shotgun sequence.
ACCESSION   DADJYG010000193 DADJYG010000000
VERSION     DADJYG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.199"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..579
                     /locus_tag="JZ382_002531"
     CDS             127..579
                     /locus_tag="JZ382_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428672.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="JZ382_002532"
     CDS             593..1282
                     /locus_tag="JZ382_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428673.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3659
                     /locus_tag="JZ382_002533"
     CDS             1407..>3659
                     /locus_tag="JZ382_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAY2428674.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTD"
BASE COUNT         1237 a          485 c          758 g         1179 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagat
//
LOCUS       DADJYG010000194         3624 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.200, whole genome shotgun sequence.
ACCESSION   DADJYG010000194 DADJYG010000000
VERSION     DADJYG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.200"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            41..2038
                     /locus_tag="JZ382_002534"
     CDS             41..2038
                     /locus_tag="JZ382_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428675.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2114..2449)
                     /locus_tag="JZ382_002535"
     CDS             complement(2114..2449)
                     /locus_tag="JZ382_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428676.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2576..2791
                     /locus_tag="JZ382_002536"
     CDS             2576..2791
                     /locus_tag="JZ382_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428677.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2828..3190
                     /locus_tag="JZ382_002537"
     CDS             2828..3190
                     /locus_tag="JZ382_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAY2428678.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3236..3562
                     /locus_tag="JZ382_002538"
     CDS             3236..3562
                     /locus_tag="JZ382_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428679.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1311 a          474 c          725 g         1114 t
ORIGIN      
        1 gttttagtct ttaacagaat tttataagga ggaaacacaa atggaaacat caatgattaa
       61 tattccaatt tatgtagatc aagaaaaatt attagagaag ccagttacaa aaaaagaaaa
      121 aactgatgta agagataggt gtttagataa ctatagagaa gttacaagag atgagtttgt
      181 agagatacta aattcatcac aatcatttat tccaagcaag acaaagaata aaggcaatga
      241 caaggagagt tttgtggaaa ccagagtaat tattctggat gtcgataaca ctgttaaaga
      301 tgagaagggg aaggtagtag atttgagtaa agatgatagt agatacttaa gcatagaaaa
      361 ggttttatct atcaatttag ttcataattc tgcgtttgcg atacaaaaaa gtattcggta
      421 ctctgaaaat ctagaaaaat acaagattgt ctttctatta aaagaagcaa ttactgacta
      481 tggtaaaatg gttgctgttt acgaatactt gaaaaagaac atccccgggt gtgatgacaa
      541 tgtaaatgct tcgaatagaa tgttttttgg aggatataag tcagatgcag tgataataat
      601 taatgaagac aacatgttag atattactga gcttccgatt gattttacca tgacatgttc
      661 taaaaattgt caaagtgatt cagggagaga gtatgaacta gtcaatgaaa ccgattttgt
      721 taagttgata aaaaatgacg acaaagaaga aatgaaacag tggtttaaag actgcctgtt
      781 tgattcctcc gacatgtctt ttaatgagat ttatgaaaga ttgttaacca ttgatatgaa
      841 taaattactt aagtcaagta aaaacttaag atgtctgttt cacaacgatc ataatccctc
      901 tgctagtatt tttaccagta aaactgggca ttccattttt tattgtcatt ccagtaattg
      961 tgatatcagt gaaaatttta ttggagttgt gatgctaatt ttaaagaaag acagtcgggt
     1021 agaaacattt aattggctat tagagaacct agacttgtat cctacacatt ttcgaaaatt
     1081 gaaagaggaa tcaaaagtac tttttgacac tcttgaatca atgaaagaca aacttttcag
     1141 gacaataagg aattatctag aggaaatgcg acgtgtctat gatgtcttgc tttctgatgt
     1201 taattttttt gatgatagta aggaaagtgt gacttgtatt ttgtcggggg aacaattagc
     1261 taaaaaaatg agttcttact atggtaaaac ttatgacata gataaatgta ataagttact
     1321 atcacttatg acttttatag gattgataaa aaaattagat gatgaagatg ttcctttgaa
     1381 gatgctcgaa tacctaaata ggttaaagga agagaaagcg ttaggaaact ttaatcataa
     1441 gagaagcaat gtctatcgat tagacgtttt taatgcaact tctgtgattc aaaaattgaa
     1501 tgaagatatc cttccacttc tggaaaaaat tcattttaca tatcaatatt tttcttatga
     1561 atgggttaag atatgtttta acgaaaaaga ggctaaaaga gtatttcctc aaaattcccg
     1621 atcagaactc agcaaggaaa aacaattgat tttagatgaa gcacatgaag ttttacaggg
     1681 cttatcgtgg caagggacat atctaatgac agagaaagaa cttattaaca ctgtttgtga
     1741 acgcctacgt aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa acagaggcta
     1801 ctttgttaaa caaggattca ctcttgaacg agcttctaag gatattaaga aactatacaa
     1861 tgtgaaagca agtagtagtt ttttaatata tttagcgaaa ggggcaggga tagatgaaac
     1921 aataaaagaa ttgatattat caggagctgg gatagttcct ctgtatcaat ttacaagtgt
     1981 aacaagagat gatggacgga gagtaaggaa gagaaaagac ccaattgtat taaactagac
     2041 aggttatcca tagctcttaa tcttatttca tctcaataca cccaccgtta aagttcggtg
     2101 ggttatttat ttttcaatct ttcaatagct gattgcgttc attttcttgc ctttttaata
     2161 gcttcatata gtcaattgtt cgaactgcat cttcagtgct aagagaacga tagagagcca
     2221 aaaactcttc ctcataatct acgacaacat tgtctagaaa atggcttaca ggggttccta
     2281 gggctctaga aagagctgtt aagctatcga ggttaggctc tgttacagaa ttttcccatc
     2341 ttgctactgc ctgttgtgta gagaagattt tttcagctaa ttctttttgt gtccacccga
     2401 gagcttttcg cttttctcta atgacctttg catattgttt ctgtttcatt aatacacccc
     2461 catctcagta tagtacaaca agttgttgta tttgtaatgg tatttgaagt tttttatatt
     2521 ttatgagttg aaatacatca tattgttgta tataataaaa caggagggga gaatcatgaa
     2581 aaaagtaagt aatctcagcc gatacagaaa agaaaaagga ctttctcaaa cggagttagc
     2641 aaaaaaaatg aatgttacac agcaatgtgt aagttcgtgg caaacaggga gaacgattcc
     2701 taaaccctat caaatgaaga tgctttctga gatattaagt gtaccaatga atgagctgtt
     2761 ttctgatgta tttaataaag taaacagtta actaataagc aattgaatga ttttaggagg
     2821 agaagacatg tattgtagaa aatgtggcaa agaaataaca gatgaaagta agtattgcca
     2881 gttttgtgga gcactacaag aaacagatga gacaaccgga acagacacag taaagaaaag
     2941 tactgagcag acaattaatg aaatgaagga acaaatcgta catggacaag agacagggaa
     3001 gctgtatttg attattggtt ggatttctat ggtagtatcg ttagcattta ttcctgttgt
     3061 atttggtgct gttgctgtta ttatgggcta cttatatcga gaaaaagatg agaaaatggg
     3121 tacgattttg atgatagcag gggtagctgg agcaatcttt ggtgtacttt tagggatgtc
     3181 aatgtactga taagagggat aagaacaaat tattttggag ataggagata aaaagttgaa
     3241 taaagtaaat atgaacaaga agtttgtcat tataggtatt gttgtcttgg ctattatagg
     3301 tattattttt tcaaaaatga caggtacatc tggattagag aagtcatcaa aaccattggt
     3361 aacacagatt ttacaagaac agtacaactt aaatcgatca tgtgatgatg tgacgattac
     3421 tcatgaaaat ggagataaca cataccgggc taaagctata ttagacaatg gaagtgctat
     3481 caacataaat attgagtact atccaaagaa agaccgtata tatgtggaga taccatatgc
     3541 tgaggtgtta atgttaaatt gatttaaatg tattatgcat accgtccaaa caggaactcc
     3601 caaaggtgta aattcactag ggag
//
LOCUS       DADJYG010000195         3569 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.201, whole genome shotgun sequence.
ACCESSION   DADJYG010000195 DADJYG010000000
VERSION     DADJYG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.201"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            155..2344
                     /locus_tag="JZ382_002539"
     CDS             155..2344
                     /locus_tag="JZ382_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY2428680.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2341..>3569
                     /locus_tag="JZ382_002540"
     CDS             2341..>3569
                     /locus_tag="JZ382_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAY2428681.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1351 a          481 c          593 g         1144 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataaccttta tgtaaatgca tgtcctggga gtgggaaaac aagggtactt
     2461 acaaggaaaa ttgcttacca agcaattaaa cacgatggat cactaaaaag aataattgct
     2521 atcacataca ctaatagagc tgctgaagaa attaaggaac gcctaagttt acttagtata
     2581 gatgatgata ttaatatttg ggtggggaca atacatcagt tttgtcttga atttattatt
     2641 tatcctttta aaatgaatct ctcaagagta tcaaaaggat ttacaattat tgatgattat
     2701 actcaaagag cttacgtcag ccaaattaat aatatgctga atttaaatct caaaacgtat
     2761 gaactgaaca aaattgattt gtcactttct catgatttta aaattaatga agttcagtat
     2821 actcaagttt ctgaaatgta tcacactatg ttggaaaaaa ataaagaaat tgattttgat
     2881 atgattttac tagtagcgta taaaatttta actgtaaaca gtaacgttgc acgtaatatt
     2941 gctagtaata taagggcgat ttatgtggat gaatttcaag atactagaga attacaatac
     3001 aatgtaatag ctaaattact tcaaaacaac cctcaaattc agtcaatgtt tgttggagat
     3061 attgaccaag caatttatgg atcattagat ggtatagcta aatcagttga agaactagaa
     3121 atgctaacta gtcacacttt tcaatcaaag acgctacatg gctgttacag gtctaatcag
     3181 agattagtag acttctactc taattttcag agttgtcctt acgagattga aagcagaggg
     3241 aataataaaa acgaccgtgg ttttataagt tatgattgca aagttactaa ggaggattta
     3301 cccaacataa taaaaaatat aataaaggaa aagattgaaa gcggcattca tgaaaaagat
     3361 atttgtgtac ttgctcctca acactatcct ctgttttcac tgggtgagta tttaaagaaa
     3421 gaattaccgt attgcaattt tgatgctata aatatatcac caattaaggt aaataatcat
     3481 agtttatttt tcaagttagc aattttgtac tttactgaat caggtgaaaa ggttcgaaga
     3541 agaaagctaa ttgcaaacga tattttaat
//
LOCUS       DADJYG010000196         3483 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.202, whole genome shotgun sequence.
ACCESSION   DADJYG010000196 DADJYG010000000
VERSION     DADJYG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.202"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(206..883)
                     /gene="nth"
                     /locus_tag="JZ382_002541"
     CDS             complement(206..883)
                     /gene="nth"
                     /locus_tag="JZ382_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY2428682.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(890..1588)
                     /locus_tag="JZ382_002542"
     CDS             complement(890..1588)
                     /locus_tag="JZ382_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAY2428683.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1856..3040
                     /locus_tag="JZ382_002543"
     CDS             1856..3040
                     /locus_tag="JZ382_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAY2428684.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3046..>3483)
                     /locus_tag="JZ382_002544"
     CDS             complement(3046..>3483)
                     /locus_tag="JZ382_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2428685.1"
                     /translation="KRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGK
                     IPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRN
                     RHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          890 a          701 c          594 g         1298 t
ORIGIN      
        1 tattggaagg cattattttc tacatcgggt attttcccaa taaaaataac aacttttatt
       61 tttatcgcaa taaattgtaa taagttacca tcctagctac tgagtatgta catttacttt
      121 ttcatgcaaa aaagataaac gaaacagctt ttctccataa taacaagata attcatacat
      181 ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt cttttttagc gccttttctt
      241 ttagacgcat ccgatttttc ccatcttgac atattgatag caaaggacat acctcgcact
      301 tgggatttct tgccgtgcaa tgatatctac caaaaaaaat cagtgtatga tgtgtcttta
      361 cccataattc ttgtggtact ttccgcatca gtgtttcttc tacttccatg accgtagcat
      421 ctagtttgca gatcctcagg cgttttgaga cacgttccac gtgggtatct actgcgattg
      481 ctggtatccc gaatgcatcc cccaatacga catttgctgt ctttctgcct acaccaggaa
      541 gagacatcaa ttcttctctt gacgttggca cttgcccatc gaaacgttca atcagctgct
      601 gtgcacacgc tttgatgttt tttgctttat ttcgatacaa accaatcgtt tttattttaa
      661 gaatgatttc atcgattgaa gcttctgcta acgcatcggg tgtcggaaaa gaggcaaaca
      721 aatctggcgt tgctttattc actgatacat ctgtcgcctg ggcacttaag atcactgcaa
      781 tcaataactc aaatggatta ttgtgcttga gttctccatg tgcttcggga aacattccat
      841 acatcgtttc tagagcttcc attgtttttt gtttgcttaa catattccgc tactcctcca
      901 accaattttt cagtgagact ttcggcaatt cttcttgttg agctgactgt tctacttctc
      961 tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc cacttgttgt tttgtacgta
     1021 gattttttct ttcccacgat agcaaaatgc ggtcaacgta attaaaacta taagcctgat
     1081 ttaatacggc ttcccgcagt gctagcttca aaatttccgg ctgataatgg tcttcttcta
     1141 gccattgtcc gatccgctgg aactcgatcg aagaaagcgg gcgtccaaat tcttgttcaa
     1201 ataattgata cactgactgg atttcttttt cttgtgtttt ttcttcgtat tttttttctt
     1261 gtgtttttaa atactcacct aatagatcat agatggggta aagattatat cgatctgctt
     1321 ttttaccgtt gacaatcgtc gtttctatct tgataaatcc atttgttacg agacgatcca
     1381 gtatttgaaa aatctgatct tgtttgattc ccatatccat agcaatcatc tgcaaatcag
     1441 ggaaagtatc tccttcaagc tgtgccatat gcaactgtag aacaaataaa aattcttcag
     1501 attgtagacc gatcctatga taatttttaa gtaaaagatt agatatgact gtttgtccag
     1561 cgcttaagta attttctaaa ttcaacatga tttctcctcc gctcaatcac agtatacgta
     1621 aaaaaaacat gggaagcaag aacaggtttc ttttctattt acttcactga tcggtcagat
     1681 ttacaatgtc gtctacttga tttttgtttg ttcatgtttt tgatgcgtat cttcactttt
     1741 cggaataaat aaattaagta gatttcgctg ttccttagaa tgttgacaag gaaacaaaat
     1801 tactatacac tatttttgtt tccaagttaa cattattgca taaaggagtt tttacatgta
     1861 cagtttcaca aataaacatt ctaaagtttt tggatacgtc tctgtcttat tcactggatt
     1921 aggatttact atcgtcagtc ctgtacttcc gtttttaacg ctgccttatt ctcactccat
     1981 acatcagcaa gctttctata tcactttgct catgtctgtt tatgctctag ctgccttctt
     2041 atctgccccc attctcggta gcttaagtga tcattttggg cgtcatccga tccttattat
     2101 cagtttatta ggatcaagta tcggatattt gctttttggt ttaggaaatt cgatatggat
     2161 gttgttcctt ggtcgtatca ttgaaggatt gacggcagga gaaatctcca ccttatatgc
     2221 ttactttgcc gacatcacgg aacctaatga aagaaccaaa gtttttggat ggatgggtgc
     2281 tttagtcggt ataggaacaa cgctgggtcc tattataggg ggtctattag ctgagttggg
     2341 gaattctgtt cctatattta ttggagcact ctttactttc ttaaatgccg tttatggcta
     2401 cacatttatg ctagaaagcc ttccaataaa aaaacgttca gtagatttaa gtttctccca
     2461 tgtaagacct tttcaccaat tacaacaatt attcaagatt tcatccgtta ttcctttatt
     2521 gacagcaggt tttgctgttt ggttggcagc tggctctctt caatctatct tttctcaatt
     2581 ttcgattgac gcctttcagt ggaaggctgg gttggttggt ttaagcttct ctttgattgg
     2641 aatcttggat actgtctcgc aattgttcat tatgcctcga ttgctcaaaa aattttcaga
     2701 acagcagatc acaagaatag ggatgttcag tgaaatcctc gcctatctat tcatcacatt
     2761 atctggaatt cttctactgc cgattttgtt tttatttggg attatctgtt atggtttcgg
     2821 tgattctatt tttactcctg tattcaatgg ccaactatcc aattctgtct cagataacca
     2881 gcaaggtctt gtaatgggcg gtacccaaag tatacaatct ctatcgcgcg tcatcggccc
     2941 gctaatcgca ggacagcttt atgctgcagc accatttctt ccgacggtat tcggattttt
     3001 gcttatgatt tttgcctatt atcactacaa taaaaaatag tagcttcatt tgctacttcg
     3061 tataaattct ctgtatcgac cgataaaacg aaatactgtg tcaaattctt cttttgacat
     3121 attttcaaat acagtagcat ctcgctgtac ccatttttga tggagttgct cgtgtctatt
     3181 ccttattttt tgtccgattg gcgtcaattg aaaatagatt tcttttttat tctcttcgct
     3241 ttgataagtg tttatgatcc ctctagctat cagttttttt gttagcttac tgatggctcc
     3301 tctagtcatg taactagcat tcgctaattt tgtcacgtta ggatcaggga ttttcccgat
     3361 atgttcaatc acttctagtt catttagagt aaatcctgca aattcttttt ccatttctgg
     3421 tttattacga tgaataatct cattagctaa atccagatat tcttctatta gcagttgtct
     3481 ttt
//
LOCUS       DADJYG010000197         3447 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.203, whole genome shotgun sequence.
ACCESSION   DADJYG010000197 DADJYG010000000
VERSION     DADJYG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.203"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..168)
                     /locus_tag="JZ382_002545"
     CDS             complement(<1..168)
                     /locus_tag="JZ382_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAY2428686.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHD"
     gene            complement(204..548)
                     /locus_tag="JZ382_002546"
     CDS             complement(204..548)
                     /locus_tag="JZ382_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428687.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            849..1070
                     /locus_tag="JZ382_002547"
     CDS             849..1070
                     /locus_tag="JZ382_002547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428688.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            1197..1973
                     /locus_tag="JZ382_002548"
     CDS             1197..1973
                     /locus_tag="JZ382_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAY2428689.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1970..2164
                     /locus_tag="JZ382_002549"
     CDS             1970..2164
                     /locus_tag="JZ382_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428690.1"
                     /translation="MTMSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRR
                     KPDKQIKPIEAALAELEKEK"
     gene            2166..2450
                     /locus_tag="JZ382_002550"
     CDS             2166..2450
                     /locus_tag="JZ382_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428691.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            2471..2659
                     /locus_tag="JZ382_002551"
     CDS             2471..2659
                     /locus_tag="JZ382_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428692.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2985..3224
                     /locus_tag="JZ382_002552"
     CDS             2985..3224
                     /locus_tag="JZ382_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428693.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            3230..>3447
                     /locus_tag="JZ382_002553"
     CDS             3230..>3447
                     /locus_tag="JZ382_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428694.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDI"
BASE COUNT         1262 a          572 c          632 g          981 t
ORIGIN      
        1 atcatgatgt ttagaagcgt gtcctaattc atgtaataag gttctttgtc ttctatactt
       61 agataaatta gcttttatta ctatggtatt catacaagca acataatggc catcggcatc
      121 aaggttttca cgctcttcta ccttaacgcc gagttcatta attatcattt caatttgact
      181 gtccaaatga ctcacctact tatttagtta tcttttttct ctaaatatgc ttcaatcata
      241 gctgatagta tttccctatc attatcggtg agcggttttc cgtcgctact cataacagat
      301 gctaaagctt cttccacggt tagttttctt tcctctggtg caactcccgc gttaggatca
      361 tctgtgcgtc ctagcaaata atctgttgat acatggaagt agtcagcaac tttttgcaaa
      421 gtttctattt taggactttg tgtcttccat ttataaatag agttttttcc aaatcctatt
      481 ctattttcta attcagaaat agaaatacct ctttttttac agagctcttt aaccctttcg
      541 aaaagcatgt caaatcaacc ttcctagagc gtacgataat aatttataca aaaagataga
      601 aaggtagttg acaaatctat ccaattgtct tatatttaac tcgtaagcta atttaataag
      661 ctaacaagca acaagaaata cacctaataa aataaacgaa ttcgaggtcg gcaaactaag
      721 aatgttaatt tttagggttt ttctaggctt atttaactgt atataaatat aagacagttg
      781 gatagaaatg tcaataaaaa ttagctaatt ttattagctt acatatttaa agaaaaggag
      841 gaaacatcat gaaacaaaat aaaaaaactc cttccgcaaa aggagcttta ataactttaa
      901 attttaaaga ccaaaagttt agtgctgttg ttgatgaaat tcatccacaa gagcatcaaa
      961 aaaattcttt tgcaactgac gagaaaaaga ctcagaaacg ctctgagaaa gttcttcgaa
     1021 taattcaatt tctgttggaa aagattcgtc attttggaag tctattttga attctttatt
     1081 aacagtcttg aatacggttt gtttgaataa ggcgtcgaca tccatattaa attcttccat
     1141 actattttca cctcgctttc tattcaaaat tatatcaaag aaaggaatga gcaaaaatga
     1201 acacaccgca aatttttaac tttgaacaac atgaagtaag aacagtaaca atacatgatg
     1261 aaccattttt tgttggaaaa gatgtggcta aagttctagg gtaccaaaat ggtagtagag
     1321 atattaacag acacgtcgat gtagaagatc ggcaaaacta ccaaaacggt acttttgaat
     1381 cgcctagagg gttaactatt attaacgaat caggtttgta ctccttaatt ttaggaagca
     1441 aacaaccaaa tgctaagaaa tttaaacgtt gggtgactag cgaagtgttg ccagcaatta
     1501 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
     1561 caatcatcca attagcaact aagttgaaag aagaacgtac tggaagacta atcgcagaac
     1621 aaaaaattgc agaatacgaa cccaaaattt cgtatttgga tagtatctta tcttctacag
     1681 attcagtaac tattagtcaa atcgcggctg attatgggat gtctccacaa cagatgaaca
     1741 aactacttca taagctaggt attcagaaaa aagtaggcaa ccaatggtta ttatgcaaaa
     1801 aacacatgag acagggttac acaaaatctc atacaactga gattccgaaa tctgatggcg
     1861 gaacaaaagt tgtgatgaat acaaaatgga ctcaaaaagg gcgtctgttc atttatgagt
     1921 cgctgaaaaa agaaggatat attccagaaa tcgatttatt agaggaggaa tgacgatgtc
     1981 acaagaattt attttgaaag tcagaattca attagcaaaa tacggaaagt ctcagaactg
     2041 gttagctgat actatcggaa tttctagacc atatatgtcg gacattatga atgggcgtag
     2101 aaaaccagat aaacaaatca agccaattga ggcagcacta gcagagttag agaaggagaa
     2161 ataacatgca aataacaatt ccagataatt tagtagtttc cgaattaact acacagatta
     2221 cgaatgctgt tcttaattca ttggacgaac gattacacct tatgaacaaa tcagtagagc
     2281 ttcctccata tccaaacaaa tcagaggtaa aaaaagtttt aggcattggt gatgacaaat
     2341 taacacattg gataagccta ggcttaaaaa cacagcagtg gagcaagtta gacatcagaa
     2401 ttgaacgatc ggaactccaa agatttttga aagaaaactt tgagttctaa aggcaaagga
     2461 gaatgatttt atgtcctaca cattgcaaca agaacatcaa attctaggtc tgattaaaca
     2521 gcgtagaaaa caattacaag atgatcgtgc agcgcttaga aaatccgatg agctatcaga
     2581 tagacaagct gaactaattg cttctgaact tgaggatttg agaatgctag aaataaaaaa
     2641 tagggagatt agattatgaa gaagacagac acacttttta taggattcat tttggggtta
     2701 ttagtgattg tagcgcacca aagtattatc ggaggaagct tgttcgcagc attgatggtt
     2761 ttaatcaatc tgcttgactc aaaagaaagg agcaactatg gcacgagaag aagcgctaaa
     2821 aatcggtaaa gtgattgctg ataattggtg ggcaaatagc cgttctatta ttttaagcaa
     2881 gcaacatatc gaaaagaaaa aagcatggca acaaataaaa aagtgactcc gccgaccaaa
     2941 gcaatgagtc actcaaaaaa tcataactaa ggagatttta acatatggaa aatgaacttt
     3001 ccactctaga tcaatatttg gccgatcctg attggggcaa atcgaattta caactggttg
     3061 ctaacaagta cgaaaaatca attgatgaag caattgttgc attgactgat gcagcttcca
     3121 taaccgctga tgacagtaaa tggaccaaga gagacttggg cattctaaaa cgaggcgtta
     3181 attggcttga agatcagtac agagaagaaa caaaagagaa gtgatatgag tgtttaaacc
     3241 actaatagat tcatactcgg cggtactaaa gaaatttaaa ggtaacgaca ttggtgcaac
     3301 aatcaacgaa gaagtaaata tcgaacggct gaaaacgatg tatgacggat atgatggcga
     3361 tcgagtcatt gaaattcgct ttattgatcc acgacggttc acagtacagc aaagaaactt
     3421 catctatgcg ctcataggcg atatttt
//
LOCUS       DADJYG010000198         3407 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.204, whole genome shotgun sequence.
ACCESSION   DADJYG010000198 DADJYG010000000
VERSION     DADJYG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.204"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            215..997
                     /gene="rpsB"
                     /locus_tag="JZ382_002554"
     CDS             215..997
                     /gene="rpsB"
                     /locus_tag="JZ382_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY2428695.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1096..1977
                     /locus_tag="JZ382_002555"
     CDS             1096..1977
                     /locus_tag="JZ382_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY2428696.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2113..2835
                     /locus_tag="JZ382_002556"
     CDS             2113..2835
                     /locus_tag="JZ382_002556"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY2428697.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2838..3395
                     /gene="frr"
                     /locus_tag="JZ382_002557"
     CDS             2838..3395
                     /gene="frr"
                     /locus_tag="JZ382_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY2428698.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1250 a          593 c          759 g          805 t
ORIGIN      
        1 attcttttta tctttcaggt aaactattga atgttgagca gaaaagtata tttgctcttg
       61 cgatacggat cttttgtctg tataatagct gagggtgctt tatgcatcag caaatccaca
      121 ttcttattgg attgtcggta ggtgcttgaa aaagtttgcc ggcaaggtag aagataagaa
      181 gaggaaaaat aaaaccaaat cggaggaaac aaacatggca gtaatttcta tgaaacaatt
      241 actagaagcc ggcgtacact ttggtcacca aactcgtcgc tggaatccaa aaatgaagaa
      301 atatatcttc acagaaagaa acggaatcta catcattgac ttgcaaaaaa cagttaaatt
      361 agtagatgca gcttatgatt acatgaagag cgttgctgaa gaaggcggcg tagcattatt
      421 cgtaggtaca aaaaaacaag cacaagaagc aatcaaagaa gaagcaactc gtgcaggaca
      481 atacttcgtt aaccatcgtt ggttaggcgg aacattgaca aactgggata ctatccaaaa
      541 acgtatcagc cgtttgaaac aaatcaatgc aatggaagaa gatggaactt ttgaagttct
      601 acctaaaaaa gaagttgctg gtttgaacaa agaacgtgaa cgtcttgaaa aattcttagg
      661 cggtatcgct gatatgccaa gaatcccaga tgtaatgtac atcgttgacc cacgtaaaga
      721 acgtattgct gttcaagaag cacacaaatt aaatatccca atcgtagcta tggttgatac
      781 aaactgcgat ccagatgaaa tcgacgtagt aatcccttca aacgacgatg caattcgtgc
      841 cgttaaattg attacttcaa aaatggctga tgctttcatc gaaggaaacc aaggtgaaga
      901 tcaagtagtt gaagaagatt tcacagaaga aaacacagca acatcaatcg aagaaatcgt
      961 agatgttgtt gaaggcgaca attcttcagc agaataattt tgaatgatgg agctgtttca
     1021 aaggctgggc gacaagcctc actctccttt gaagcagctt tttttaaaaa gaaaaacaca
     1081 caggaggagt taaacatggc agacgttaca gctaaaatgg taaaagaact acgcgaaatg
     1141 actggcgtag gaatgatgga tgcgaaaaaa gcattagttg aagtagaagg cgacatggaa
     1201 aaagcagttg atcttttacg tgaaaaagga atggcaaagg ctgcgaagaa aaatgaccgc
     1261 attgcagctg aaggattggc ttcagttgct gtgaaaggta acactgcagc aatcgttgaa
     1321 gtaaactcag aaacagactt tgtttctaaa aacgaaatgt tccaagattt agtaaaagaa
     1381 attgcagaac ttgttgctga aaacaaacca gcagacatgg aagctgcaat gaaaatcaaa
     1441 acagataaag gtacaattga atctgacttg atcgaagcaa ctcaagttat cggagaaaaa
     1501 atcagcttcc gtcgctttga agttgtagaa aaagacgaca atgctgcatt tggtggatac
     1561 ttgcatatgg gcggacgtat tgccgtattg actgttctag atggaacaac tgacgaaaca
     1621 gttgcaagag acgtagctat gcacgtagca gctatcaatc ctcgttatgt aaacgaatca
     1681 caaattccag aagctgaatt agaacatgaa aaaactgttc ttacagaaca agcattgaac
     1741 gaaggcaaac cagcgaatat cgttgaaaaa atggtagaag gacgcttgaa gaaattcaaa
     1801 gcagaaatcg ctttggtgga ccaaccattc gttaaagatc ctgatatgac tgttgaaaaa
     1861 tacgttgcat caaaaggcgc aactgttaaa acattcgtac gtttcgaagt tggcgaaggt
     1921 atcgaaaaac gcgaagataa ctttgtagaa gaagttatga gccaagtgaa aaaataattc
     1981 tgttgaataa cagacgggaa cgcacggttt gtgcgctccc tttttttaag caaaacaaga
     2041 aaaaccagtc ttagagtgtc gaaatcatct aagtatggta aaatggcaat agaaaatatt
     2101 ggaggaatga acatggtcac accgaaatat cagcgtgtag tcttaaaatt gagtggagaa
     2161 gctttagctg gggacgcagg atttggaatc aaaccaccag taatcaaaga aatcatccaa
     2221 gaaatcaaag aagttcacga gctgggcgtt gaaatggcaa tcgttgtggg cggcggaaat
     2281 atctggcgcg gacaaatcgg agcacaaatg ggcatggaac gtgctcaagc tgattacatg
     2341 ggtatgttag ctacagttat gaatgcctta gctttacagg atacgttgga aaacgtgggt
     2401 gtaccgacac gcgtgcaaac ttcgattgaa atgcgtcaaa tcgcagagcc atatattcgc
     2461 cgtaaagcag aacgtcattt agaaaaagga cggatcgtta tctttgcagg cggtaccggc
     2521 aacccgtact tctcgacaga cacaacagca gcattacgcg cggcagaaat cggcgcagat
     2581 gttatcctga tggctaaaaa caatgtagac ggtgtttatt ctgctgatcc gaaagtagat
     2641 gcaaatgcag taaaatttga agaactgact catctggaag ttattgccaa aggattacaa
     2701 gtcatggatt caactgcaag ttcactaagc atggataatg atattccact gcttgtcttc
     2761 aatttaaatg aacacggaaa tattcgccgt gcaatcttag gcgaaaatat cggaacaact
     2821 gtaaggggga aataaaaatg gcagatgcaa ttatgacaga agcaaaagaa aaaatgaaaa
     2881 aagcagcaca aaatcttcaa cgtgaacttg ggcaaatccg cgcaggccgt gcgaacgcta
     2941 gccttttgga ccgtattact gtgaactact acggtgcgcc aacaccattg aaccaaatgg
     3001 catcaatcca aattccagaa gcacgtgttt taatgatcac accttttgac aagagcattc
     3061 ttcaagatgt tgaaaaagct attatggcaa gtgatatcgg aatcagtcca accaatgatg
     3121 gaaatgtcat tcgcttagtt atcccgcaac tgacagaaga acgcagaaaa gaattagcta
     3181 aagatgttaa aaaagaagca gaaaatgcaa aaatcgcgat tcgtaacatc cgtcgtgatg
     3241 cgatcgatga atataaaaaa caacaaaaaa atggtgatat taccgaagat gatttgcgcg
     3301 gattagaaaa agacgtacaa acattgacag ataacagcat caaagaagta gataagatcg
     3361 ctgctgataa agaaaaagaa ttattggaag tttaataaaa tcataaa
//
LOCUS       DADJYG010000199         3306 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.205, whole genome shotgun sequence.
ACCESSION   DADJYG010000199 DADJYG010000000
VERSION     DADJYG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.205"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..84)
                     /locus_tag="JZ382_002558"
     CDS             complement(<1..84)
                     /locus_tag="JZ382_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2428699.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNS"
     gene            245..934
                     /locus_tag="JZ382_002559"
     CDS             245..934
                     /locus_tag="JZ382_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY2428700.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            948..2450
                     /locus_tag="JZ382_002560"
     CDS             948..2450
                     /locus_tag="JZ382_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2428701.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2463..2939
                     /locus_tag="JZ382_002561"
     CDS             2463..2939
                     /locus_tag="JZ382_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY2428702.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(3036..3146)
                     /locus_tag="JZ382_002562"
                     /pseudo
     CDS             complement(3036..3146)
                     /locus_tag="JZ382_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1099 a          448 c          626 g         1133 t
ORIGIN      
        1 agaatttaaa ataaaatcag cgactttttt ttcagacttc gttaaaaatg gataattttc
       61 tttaattaaa tagatataac tcatacaaac tcctctacaa tttcaacttt tctccattat
      121 actccaaaat aataaaaaaa actcctaaat actccatttt tatctttact tttatgaaag
      181 cggatgctat attgtttttg gagttgttat ccaaaaataa taaaaaattg gagtgtttag
      241 gataatggat aaaaagaggt ttttagatct tatttcaaaa ggtttgattg tttcctgtca
      301 agcattacct ggtgaaccat tttacaaaga ggaagaaagt ttaatgcctt attttgcttt
      361 ggctgctcaa agggctggag cagttggtat acgtgcaaat tctgttcgag atattattga
      421 aattagaaag tgtgtatcat taccaatgat tggaattatt aaaagagatt atccaccaga
      481 atctccattc attactgcca ctatgagaga agtagatgaa ttagtagaaa ccggtgttga
      541 ggtaatcgct cttgattgca ctcttagaaa tcgacatgat ggactatcta taaattcttt
      601 tattactaaa ataaaagaaa aatatcctca tcagttattt atggcagata tttctacatt
      661 tgaagaaggc aaaaatgcat ataaagcagg aatagatttt gtaggaacta ctttaagtgg
      721 atatactgag gaaagtaaaa aacaagatgg gccagatata gatttgatta aaaaattggt
      781 agaagaaggg atttgtgtaa ttgctgaggg aaggattcat actccagaac aagcaaaaga
      841 gataaaaaaa ataggagttg caggtattgt tgtgggtggt gctattactc gaccacagga
      901 aattgcatca agatttgtta aagcattaag ttagggggag aaataaaatg tttaaaaaat
      961 tttcacaatt aggacgcgct ttcatgttac caattgctat attaccagta gccggattac
     1021 tattgggact gggaggagcg ttaacaaatg agagtgctat aaatgcttat ccatttttag
     1081 atcaaccgtg gttgcataca attttaagta ttatgagtta tgctggaaat gcagtttttg
     1141 caaacctagc actaattttt gcaattggta ttgctgtagg acttgcaaat ggagataaag
     1201 gaacagctgg tttagctggt ggagtagctt atctagtata tactgcaact attagtgggt
     1261 ttcttgaact tttttcagca aaagatgcaa cactagatac aggtgtagtg ggatcaattg
     1321 ttgttggtgg tactgttgca tttttacaca atcgttatag aaaaattgaa ttaccacaat
     1381 tcttaggttt ttttggagga tcaagattta ttccgattgt ttctagttta gctgcaatca
     1441 ttattggttc tttcttttat ttaatttggc caccaattca aaatggttta gtagttgctg
     1501 gtgaacaaat tgcccaaatg ggtagtttag gaactttttt atatggtttt ttattgcgtt
     1561 taacaggtgc tgttggacta caccacacca tttacccttt gttctggtat acatctttag
     1621 gtggtaccga aactgtagct ggatcaacaa ttgctggagc ccaaaatatt ttctttgccc
     1681 aactagctga tccaaaccac actggtctgt ttacttatgg tactagattt tttgcaggaa
     1741 gatttgcaac aatgatgttt ggattaccag cagcatgtta tgcaatgtat cgtgcaattc
     1801 ctaagaaaaa tcgtaaaaag aatggtgggc tttattttag cggggccctt acatcatttt
     1861 taactggaat tactgaacca gttgaatata tgtttttatt tgttgctcct tggctttatg
     1921 tgattcatgc attcttagat ggattatctt tttattttgc tgatatctta aatattcgtg
     1981 tagggaattc cttttcaggt ggtttgatcg actttttatt attcggtgta cttcaaggga
     2041 atgacaaaac taattggtta aaattaattc cttttggtat tgcatgggca tttatatatt
     2101 tctttgtttt tacattcttt atcaaaaaat ttaaggtagc tattcctgga atggaagaag
     2161 aatcagaagt tcattctgaa aaagtaataa acaaaaatac atcattacat gaagagtctc
     2221 ttatcattat tgaagctctt ggtggaaaag aaaatattga gtctgttgaa gcttgtgcaa
     2281 ctcgtttgcg tgttgctgtt acgaatgggg agattattca aaaacaaaca ataaaagaca
     2341 ctggtgcaac tgctgtcttc gaagtgaagg gtggcatcca agctgttttt ggagggaaag
     2401 ctgacttatt aagtcaagaa atcaatcaaa tattaggaaa agataattag gaggttttta
     2461 atatgtttga aattttcaaa aagaaaaaac aagaaacttt ttatgcacct tgttccggaa
     2521 aaatcatttc catcacagaa gtaaatgatc ctgtcttttc acaaaaatta ctaggtgatg
     2581 gttttgccgt catacctgat ggttcaaaaa tttatgctcc attggctggt cgagtaacaa
     2641 atatatttcc tactaaacat gctattaata taaaaagcaa ctcaggcatt gattatttga
     2701 ttcatattgg aattgataca gttgaattac aaggagagcc tttttcgatt tgtgttcaag
     2761 aaaatgatga ggttactcct gaaacctcgt tggttgatgt ggattttgac aaaataataa
     2821 aatctggtaa agatacttct gttatagttg tttttccgga gaaaaatcag ttcaatgatt
     2881 taataataga taatggtcga attgctcatg ggaattcatg tggaaaaatc actttttaaa
     2941 aaagtgtata taaaaagcta taaaataagc taaataggat atttgtcaaa tgaagttggt
     3001 gaacagaaag aactgttttc tttctgttca tttttttatt ttattagtaa tgattcccca
     3061 tgccaaatca tgatccgtaa tttaaagtta taaaagcttc gaaatccatt ggcatttcgt
     3121 tggaatacct tgatatgatt attcaaatat aaaatataga gagaagagag agagggaaga
     3181 tatttataaa gggcaaagtt atgtttactc aacttaggaa atagaaatag atcaatttat
     3241 attctcttgt caatcgaata tgaaccttag cactatatac caaatatagt aaaatgctga
     3301 aagatg
//
LOCUS       DADJYG010000200         3226 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.206, whole genome shotgun sequence.
ACCESSION   DADJYG010000200 DADJYG010000000
VERSION     DADJYG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.206"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            43..255
                     /locus_tag="JZ382_002563"
     CDS             43..255
                     /locus_tag="JZ382_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428703.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(533..2332)
                     /locus_tag="JZ382_002564"
     CDS             complement(533..2332)
                     /locus_tag="JZ382_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY2428704.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2456..3052)
                     /locus_tag="JZ382_002565"
     CDS             complement(2456..3052)
                     /locus_tag="JZ382_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY2428705.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1155 a          590 c          574 g          907 t
ORIGIN      
        1 taaagaaaaa acaaaataaa aataaagaaa agagggaatg aaatggtaaa agcagaagta
       61 ggaagatctt atatatgcaa accgatcgga gtatctcatg aactaatcgg ctgtattgaa
      121 cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt atgaccaacg agactatcaa
      181 cttatcaatg aaagcaagta tcgcatgctc gtaaaattct gtgatattag cgcacctgct
      241 gaattggttt cttaaacagt atttttgaga attcgcaaga actcccttat aatataaata
      301 aagacagaag ggagcgagaa gatatgccaa aaagaaaaag ttcagggaaa atgggaatta
      361 ttcttgcagt gatctctgca attcctgcta ttttagctat tgttcgtgcg atgaaggaat
      421 caagagaaag ttaagcacac attatgagcg tcaaacaaaa aacgcatata cctgcggtgg
      481 caggttatgc gttttttgtt gtataaagac ttttttcatt aacgattttt gattagccga
      541 ctcccaaaac aaaatttagc agtttatctg aataagtgat ttcttcatct gtttgtttca
      601 cactttcatt tagtagtccc atctcatcgg ttatgatacc atccacacca taaaacatca
      661 tgcgactcat agcatctgaa tcgttgattg tccatacata tacttctttt ccgtcttggt
      721 gggcagaatt aatgaagtta cggttgatcg tactatattc cattgtcaga aaatcagcag
      781 gcgtcacagg gggcccaaca atattgaaag gtaagatgta gcccacataa aagtttggtt
      841 ctgcttcttt taaatctgaa acggtttggt acgtcaaact ttgcaaaata tgatggttct
      901 ctaagataac tttgcggtat ttatggacaa atcgttcgac aaggtcatcc gaatcttttt
      961 tagaggtctt gatctcaata agtaattttt ggtttagctt atgtgctttt tccaaataag
     1021 aatcaaaaga acaaacaggg gcttgcactc cgttttcagt aactgtaaga tttgtcaatt
     1081 ctttcaatgt aagatcctgt ggtgttccat ttacccctgt taagtgacga agatttgcat
     1141 catgcatcac tacgaaatga ccatcttttg tttcctgaac atccatctct acataatcag
     1201 gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt attttgcgcc ccattttggg
     1261 tactcacacc tctgtgagag acagtcactg gttttgtctg aacagctgaa gtcagataat
     1321 ccatattgta aagacagacg ccaataccga aaaaaactgc aaaaagagtc aaaccagtgt
     1381 ttttcagcgc agagaaacgc aagttggggg cttgtgggat aaaccactta gggatctcag
     1441 gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata aaaaatccct acggtagaca
     1501 tcacgatatt gaataataaa atcccttgta aaagcgtcat agcaaacaca gcgctgatca
     1561 atgcatagtc tgggaaaaat tgttcaacca tactttgtgc caaaataaca acgatatatc
     1621 ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat aaattggccc gtgatggcca
     1681 aaagacgcga tttggtcaat gaccagcttt cccgaatggc tgctcgaaaa ggacgatctc
     1741 gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc aatatagcca aggaaaatat
     1801 aaacgagtaa aaaggaactg acgatgatcc aacgattagc aaaaataaaa tccataatga
     1861 aagcggggat ttttattttt gacagtagat cagaattaaa acttaatccg ctgatcggta
     1921 gaatcaataa aaaataagca aggaaaaaca aaaaagtaat gggtctcact ttcttcagct
     1981 gaagtatcgt caagtgtaag agttgcttca aagagattgg ttctttcttt ttaataaaaa
     2041 aaacactcat caaaagaaaa gtaaattcga aataaactaa cagtaaaatc gtcatcaaaa
     2101 ctacgattaa cgagagtaga accccaggat gttgagaaaa aattgaagga atattgtcat
     2161 aagacaaata gtcaattgcc cctcggtgta aaataaaacg ggtcgtactg ctcaataagg
     2221 gtaaacaaat aaataaaata aagccatgca tcagcaagac atcacggaaa taagcttgtg
     2281 ttccttttaa aaagtcccaa gcattaccta agctacgttt caaataattc atcctatcac
     2341 ctgctcttca cattgtagcg caaatctaat gaaaaaggaa agggtgcagt gtgttttgca
     2401 gaccctttcc cagatttaac attgtcaatc aatgactaat tttacaaaat aaatattatc
     2461 caagaagacc acttagatat tgacctccat aaatcaaggc catactatat aagaaaatcg
     2521 acagtggttt agcaatcaaa atgattagtg tatatcgttt cactgacatg tttgtcaatc
     2581 cagccatcaa acataacgca tcgtcaggag caactggaag aaaaatagct agagcaaaca
     2641 gcttctcaaa acggttttga ttatccagcc agccaatata tttgttatat gtcttatcgc
     2701 taatcatgga aagtatgaat ggctttccat aacgacgccc tagcagaaaa atgataatcg
     2761 aaccaatgca gattccaata tagttataga taaatccagc ataagggcca aatatcagga
     2821 caccagctgc agtactgatc cctccaggaa tgattggaat aactacttgc aggatttgga
     2881 tgaagacaaa aataatcggt cctaaaatga tggaatcacc tactaatcct cttaacgcat
     2941 tcaaatcttt gaaaacacct aaattaataa aataaatcgt cagcgcgatt gataggccta
     3001 atccgataat ggatatgaaa tttatcagtt ttcgagaggc agcaatattc attattcatc
     3061 aactccttag aggttctttg ttaactatac tttactagaa aagcaactaa ctgcgccatt
     3121 ttttgatatg acagaaaaca aacaattttg taagttcatc ataagtataa aagattgatc
     3181 tcaaaaaaac atccaactaa agacttattt aataacttgt tttttt
//
LOCUS       DADJYG010000201         3221 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.207, whole genome shotgun sequence.
ACCESSION   DADJYG010000201 DADJYG010000000
VERSION     DADJYG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.207"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            301..585
                     /locus_tag="JZ382_002566"
     CDS             301..585
                     /locus_tag="JZ382_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428706.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="JZ382_002567"
     CDS             588..1004
                     /locus_tag="JZ382_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428707.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="JZ382_002568"
     CDS             complement(1141..1860)
                     /locus_tag="JZ382_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428708.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="JZ382_002569"
     CDS             2535..2630
                     /locus_tag="JZ382_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAY2428709.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="JZ382_002570"
     CDS             complement(2932..3177)
                     /locus_tag="JZ382_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428710.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1073 a          596 c          506 g         1046 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaggtcgga ttctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DADJYG010000202         3221 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.208, whole genome shotgun sequence.
ACCESSION   DADJYG010000202 DADJYG010000000
VERSION     DADJYG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.208"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(14..706)
                     /locus_tag="JZ382_002571"
     CDS             complement(14..706)
                     /locus_tag="JZ382_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY2428711.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="JZ382_002572"
                     /pseudo
     CDS             791..967
                     /locus_tag="JZ382_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="JZ382_002573"
     CDS             complement(1143..1712)
                     /locus_tag="JZ382_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY2428712.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="JZ382_002574"
     CDS             complement(1786..2109)
                     /locus_tag="JZ382_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428713.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="JZ382_002575"
     CDS             complement(2263..3075)
                     /locus_tag="JZ382_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428714.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          894 a          648 c          553 g         1126 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca t
//
LOCUS       DADJYG010000203         3183 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.209, whole genome shotgun sequence.
ACCESSION   DADJYG010000203 DADJYG010000000
VERSION     DADJYG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.209"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(906..1424)
                     /locus_tag="JZ382_002576"
     CDS             complement(906..1424)
                     /locus_tag="JZ382_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAY2428715.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2542..3183
                     /locus_tag="JZ382_002577"
                     /pseudo
     CDS             2542..3183
                     /locus_tag="JZ382_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1098 a          570 c          489 g         1026 t
ORIGIN      
        1 tagattaata aaaatactaa agttatttgt tcttttatta aatacaccta caacttataa
       61 attgatggta tttagataac acgaaagaat aaaaataaca gctgatagta aagtttgttg
      121 acgattgcta ctcaatcgaa agtgagttag tgcgtatgaa cttgcttctg aaaacttatg
      181 aaatgaaaga ccttttacat cttgcagaaa tcggtactgg gattaaacta agcaataaaa
      241 actaatgttc ccctcattct ttgaacttct actatttgat ttatttattc tgataaatga
      301 cgatttcacc ttcaggatct tcaaatcccg tatagaattt atttaagatc tcttcagtat
      361 cagtaatagg ataggcgata ggccacatac cctttgatgg ttagaataaa ctgctcaaaa
      421 tctgtatcgt atgttggctt tagcatatta acaaaggcta tctccaaatt ataaaccata
      481 agcgttgaat cgttcttaac aatttcaatt cttcctaacc caaagtcaga agatataatt
      541 accggacttg ccttaccctt catgtgggtt gttgagattc catatttaaa gggaattgtc
      601 ggtactatcg gcggtctgaa aaacatccca aaaataaaaa aatctttccc ctgctagtct
      661 cctaagtccc tttgtagcgg atatggcacc ataatctcat tttatcgaag tgagtcatgg
      721 tttatctgtt ggccaaccaa aattgataca atctaacgat gcaccctcta cggcattcta
      781 ggggagtgca cttgcattca cccaataaaa accggcagtt agcaaagaac ttatgtagtc
      841 gtatgttctt actgttaaca tacaagaggt ctcctttcca caattgctta gcagtaaacg
      901 ggaatttaca aagctaatct cttccatttt cttatctttg ttctaaaagt cccaaatgga
      961 gcaactgtat ttacatgaat aaatttatat acttcccata cagccgtttt ggttgcctcg
     1021 tctgcccatt ttcttttgtg tgcagtgaat agatcttcat cactcattga atcaatcatc
     1081 atgttaatat catcaatatt acgattgaga atttcctcta attctttcaa tggtagatgt
     1141 gcataggtat ttgtaaacca cttatataac tctcctaact gattccattt aaattgatca
     1201 gaaggcgttt taacagataa tccatttctt tcatcatttt cccattttag aattaaattc
     1261 gtccaaccaa cttgatatgc taaattttca gcaggtgttc tatccacttc ctcaactctt
     1321 ttatccttca attcttcagg aatagtttca aattctaaaa tatatttagt ataagtccta
     1381 tttatttctt tttttaattc ttctttattt ttatatgttc tcattatatt acctccataa
     1441 attcctaatt gtctccttta attcatctat aatttcatcc acgatatttt aatccgttct
     1501 acaatgcata tctgatatgc attgactgtc taactcatat gtgaatgatt tataaaatat
     1561 cccaacgtct catctaaaga aatgttgatc gtcagcaagg agctaaaaaa tacgaatata
     1621 tttcttttgt agtaaaatcg gactggtttg catatcattt tcttcgtaaa tatcaaaatc
     1681 atgttaacta ctatcaatta ctaataaaaa ctaccacttt aagcgttaag aactgttaag
     1741 tttagttaag tgaggatagt tcacaaaatc cattgttaaa cttcacgaaa gtggtaaaac
     1801 tcaaaatgct ttatatagga ttacagcatc gctctttctt cgattgcgcg ttgggtaaaa
     1861 aatattatga agtaaaaatt gacgatgaca cgttccttac ggctcatcag atgaaacagc
     1921 tacaaaaacg aaatgatcaa ctcgaagaaa atttaattct aaaaaagcga ttgccatatt
     1981 cacgcctcgc tcgacgaact attaaaggtc aagatttacc tgaagatcat cctttttagg
     2041 aaattgaagc tttttatgtt gcacaatgca tttataatac tacaatgatg ttttcaccag
     2101 atattcttct agggtgcggt gtaatgaaac agatacactt gcaaaagaaa attcaccgcg
     2161 aatttactaa aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg
     2221 gatcaagctg gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa
     2281 ttatagaaaa ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct
     2341 tattctgaaa cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag
     2401 tactatttta tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac
     2461 ttagcttgga taggctacaa ttaactagac agaaaaatta aggtgtgtag actagaagaa
     2521 aacataccag gaggaatttt tatgtctaag agaacacgaa gaactttttc acaagaattc
     2581 aagcaacaaa tcgtcaatct ttacttagct ggaaagccac gtgtagaaat cattcgagaa
     2641 tatgaactaa cggcttcagc atttgacaaa tgggtaaagc aatctaaaac gagtggttca
     2701 ttcaaagaaa aagataatct tacgcctgaa caaaaagaat tgttagaact acgtaaaaga
     2761 aaccagcaat tagaaatgga aaatgatatt ttaaagcaag cagcgctgat attcggacga
     2821 agagacaagt aatcgatgcg aataagcatc tttaccctat atcagcgatg tgcagaatat
     2881 taggtctatc acgtcagtcc tattattatc aatcaaaacc aaagaaagac gaatcagaac
     2941 ttgaagaagt agtcgctgaa gaatttatcc gcagccgaaa ggcctacggc tcaagaaaaa
     3001 taaaaaaagc cttatcaaaa cgaggcattc agatcagccg acgaaaaatt agtagaatca
     3061 tgaaaaatag aggattaaaa tcgagctata ctgttgctta ttttaaagta catcattcta
     3121 cttgcaatga agccaaaacg acaaacgtat tgaatcgtaa attcttaaga gacaacccat
     3181 tag
//
LOCUS       DADJYG010000204         3128 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.211, whole genome shotgun sequence.
ACCESSION   DADJYG010000204 DADJYG010000000
VERSION     DADJYG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.211"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            103..1347
                     /locus_tag="JZ382_002578"
                     /pseudo
     CDS             103..1347
                     /locus_tag="JZ382_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="JZ382_002579"
     CDS             1360..1992
                     /locus_tag="JZ382_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAY2428716.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="JZ382_002580"
     CDS             2197..2424
                     /locus_tag="JZ382_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAY2428717.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="JZ382_002581"
     CDS             2493..2759
                     /locus_tag="JZ382_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY2428718.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="JZ382_002582"
     CDS             2749..3018
                     /locus_tag="JZ382_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY2428719.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DADJYG010000205         3119 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.212, whole genome shotgun sequence.
ACCESSION   DADJYG010000205 DADJYG010000000
VERSION     DADJYG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.212"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            42..797
                     /gene="fabI"
                     /locus_tag="JZ382_002583"
     CDS             42..797
                     /gene="fabI"
                     /locus_tag="JZ382_002583"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY2428720.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(907..1920)
                     /locus_tag="JZ382_002584"
     CDS             complement(907..1920)
                     /locus_tag="JZ382_002584"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAY2428721.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            2085..3005
                     /locus_tag="JZ382_002585"
     CDS             2085..3005
                     /locus_tag="JZ382_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY2428722.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          911 a          636 c          598 g          974 t
ORIGIN      
        1 tacgactatc aaatttttat cagaaacaaa ggagagtcat tatgtcattt ctagaaggaa
       61 aaaaaatcgt cattatgggc gtcgctaaca aaaaaagcat tgcttggggt tgtgctgaag
      121 caatgttgaa acaaggggca gaaattatct atacttacca aaatgaacga atgaaaaaat
      181 cattagtcaa attggtcggc gaagaagcat tcactgtaga atgtgatgtt tctagcgacg
      241 aaagcatcga aaaagcaatg aatgccatcg gtgaatatgc tggggagatc cacggccttg
      301 tccatgcagt tgcttacgcc aacaaagaag aattgtccgg aaatgtgtct gatatttcca
      361 gagacggcta tcaattagct caagatatca gcagctactc tttaattgct gtatcaaaat
      421 acgcacaacc gtttcttgca ccaaacagcg ggatcgttag catgacttat ctaggttcag
      481 aacgcgcaat cccgaattat aacatgatgg gtattgcgaa agcttctctt gaagctgcta
      541 tccgttattt ggcagctgag ttctcgccta aaggaattcg ggtcaatgga atctcagctg
      601 gggcaatcaa aacattagct gtcacaggtg ttaaagacta tcaaaaactg atccagttat
      661 ctgaatctcg tacgcctgat cagcaaggtg tgacgatcga agaagtcggt aatgcttgtg
      721 ctttcttaat cagcccactt tccagcggaa tcatcggtga tattatctac gttgacaaag
      781 gtgtccatct ttcataaact tttgttgcat tctttaaagt aaaacatata aaaatcagga
      841 aaaaactttt taaagtctct tcctgatttt ttctttttac gtttttttca actcattact
      901 ttgaaattat tccacgactt caatccagcc ttctggtgct tctatgtctc caaattgaat
      961 acctgtcaat tcatcataca accgttttgt tactggtcct acttccgttt cactataaaa
     1021 tacatgaaga ttgttattgt gataaatccc accgattgga gaaataacag ccgctgtacc
     1081 acatgcacca gcttctgcaa attgatctag atcatcaatg aaaatatctc cttcaattgc
     1141 ttgcagtcct agtcgttctt cagctaactg caacaaggaa tatttcgtga tacttggtaa
     1201 gatagagggc gattgcggag tgatgaattt tccgtctttt gtaattccaa agaaatttgc
     1261 tgcacctact tcttcgattt tcgtatgtgt ggctggatca agataaacac aatctgagta
     1321 atccagttct tttgcttctg ttcctggaag taaactcgct gcgtaatttc cgccgacttt
     1381 tgctgcacca gtccccttag gagcagcacg atcgtaatca gatacaataa aattcgttgg
     1441 ttttagtcca cctttgaaat acgctcctac tggcgtacag aagacgacaa ataaatattc
     1501 tggagctgga tggacaccaa tgttttcacc gaccccaatc agtaaagggc gcaagtataa
     1561 tgtagcgcct gttccatagg gcggcacaaa atcttcattt gcttttacca cttgcttgac
     1621 agcatcaacg aacatctttt cagggaccat tggcatcagc aatcgtctag cactttgatt
     1681 catccgtcga ctattttgtt caggtcgaaa gagattgacc ttgccgtctt ttcgacgata
     1741 agcttttaga ccttcaaaac attgctgacc ataatgaagc gctggagagc cttcatgaat
     1801 cgtcaggtaa ttatcttctg tcaattctcc ttcttcccat tgtccgtctt tccatttggc
     1861 aataaaacgc caaggagttt tgatataaga aaaacctaga ttgttccaat cgatttgcat
     1921 atacttacca ccttcgttat tttctccaca ttttatcatt atccatatta tttgtcattt
     1981 tattttcagc ttttttcctt cattatagtt atttttgcta tttccttttt tgaaaacttc
     2041 ttggtacaat acagaaagaa tcaaatttta gaaaagagga ttttatgttt tcattattag
     2101 caactgccgg tacgacagac tcttctggcg agacattgac agatcaagct gtcagcaaag
     2161 taaatgcgtt tcagcggttt tgggacggga ttgattggga cgccattttg gctgccttta
     2221 tccaaaaaag tttatatctg atttttttga tcgtcctttt ttggattctt caccgtgtgg
     2281 gaaaatatct gattgataag tcttttaatc agtattctaa aaaagccatt ctaaacgaga
     2341 gccgcttaaa gactttacat agtcttttaa atactatttt tcattatacc cttggtttct
     2401 tttttatcta tgcaatttta tctgctatcg gtgtgccgat cggatcactt ttggctggtg
     2461 ccggaattgc aggtgtggca atcggtcttg gtgcccaagg gttcatgagt gatgttatta
     2521 ccggattttt tattatcatg gaacaacaga tggatgttgg ggactacatc aagctagcaa
     2581 acttaacgat tgaaggaacc gtcgtatctg ttggtatccg tacccttcag ctgaaagcaa
     2641 cggatggtac tgtccatttc atcccgaatc gaaacatcac cacgatcagc aatctctctc
     2701 gtgcaaatat gcaagtactt ttagatattc ggattgttcc tgaagaagga tatgacaaaa
     2761 tctatgagat cattgatcga gtaaatcgta cattagctga aaaataccaa gaagagctcc
     2821 aaactgaacc ttctatcttt ggattagtgg atcttggaaa cagcaatttt gcgattcgta
     2881 cggtctgcta cgcattgaac ggaaaacaat atgccttaaa agaagagttc ttgagctcct
     2941 atgtcaaaga attgacagct tctggtttca cgattccgaa cagtccaatt gctgtcggaa
     3001 aataatttgg acaaacgatg caacgaatct cactaaaaag agcgtgggac aaaagtaaag
     3061 aacacttttg tcccacgctc taaacacgta taaacggcgg aagcagaagc aactccttc
//
LOCUS       DADJYG010000206         3112 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.213, whole genome shotgun sequence.
ACCESSION   DADJYG010000206 DADJYG010000000
VERSION     DADJYG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.213"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(31..594)
                     /locus_tag="JZ382_002586"
     CDS             complement(31..594)
                     /locus_tag="JZ382_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY2428723.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(609..1652)
                     /locus_tag="JZ382_002587"
     CDS             complement(609..1652)
                     /locus_tag="JZ382_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428724.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1785..2189)
                     /locus_tag="JZ382_002588"
     CDS             complement(1785..2189)
                     /locus_tag="JZ382_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAY2428725.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2422..3057)
                     /locus_tag="JZ382_002589"
     CDS             complement(2422..3057)
                     /locus_tag="JZ382_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAY2428726.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT          941 a          654 c          531 g          986 t
ORIGIN      
        1 ggtataaaat ggatgctagg tccgttatat ttactcaaaa ttgattctac gaatagatct
       61 tcttatcatt tctattttag tagtcatgcg ttgttgttct tctaaaggtg gtaatggtat
      121 aagtagttta gataaatttg taccatttac atttggttgc ccagttccgt aagaaatttt
      181 ttctaataac ttccaatata atggcgagtc taagaagcag tctacgtatt ctgataataa
      241 tttctcaact aacctaattc gaatcaagta agaagcaaaa acagattcct cacttatttc
      301 cttaaccaag aaactttttc ccatagttcc tcctgtcctt gctatcaaaa tgtcattttc
      361 ttccaatctt aaatcaacta gtttagaatt actaatatca cagtaaggca cactggacca
      421 atttacacgt ccctcttgta tatctgtgat tctcaaaaac ttaacattgc cttgtttttt
      481 cgccgaatct gtatagccat attgaatctt agtagaaata cttcctaaat aaacccatag
      541 tcgaacatcg ctagatgatt tttcttgtcg ttctttatga acaacagtga tcatcttgag
      601 ttgtccgact attttttagt tgcagcaatc tttttgggac gcttatttgc atgaagcaac
      661 gttgctaagt ctagtaataa aacattggct aacacaggat tggctttctc gcgatacgct
      721 gcggcgccac tttcaaacaa tactgctaga cgatcttcta caacaacgat ttgttccaaa
      781 tacggtggaa atttgattcg ttttaggatc cggctaggag agtcccgctc ggacactaag
      841 agttccgagg tataaggacc ataagaacga ctggctaaga gataatggcc ataaaaagcc
      901 acaccttgaa tcttatcaga tgtttttctg ctcattgtca cggttggttg gattttgtct
      961 ttgcggcgaa ttgctgtttt catttcagct atcgtcggca atagatagaa agtcaactgt
     1021 ccttcacctt ttagtgtgaa gttgccagat accagttgat cattgttttg agtcaaatac
     1081 gatgcttgtg gaatctctga gagatcagtt gtcagccaat aagcgattgg ctgtcccgtc
     1141 tcttcagatg tatctgctaa gatatcgatc aaacgtaaag cggctactcg tccagtagca
     1201 gatcctgtga ttgtcaacca taaaagttcc cgttttgtat catatactaa gccccctaca
     1261 tgtggccgtt taggtaaaat cagcagtttg atcggctgtc ctgtttctct atccagtaca
     1321 tgaataatag aatgatgctg atggtcatag cagtaggcag aaataaacac aaaatcttga
     1381 gaaaaagcca acccttgcgg cgtcatccat tgacaaacct cattttcgcc ggtttccggc
     1441 accaccattc ttgtttctgt taatccagga atgttatatt gttttgcttc agccttttcc
     1501 caagctgctt gaacaatagg ataccgtttt aacttttttt caatatcctg atgtatccgt
     1561 tgctgcttcc aatgcttaaa ctttaggaca tagtaaacaa gaaggccaat gaccagcaac
     1621 actaaaatca ttattgctac ccgctgtgtc atttttttgc ctccttgttc tattcgatca
     1681 atcgtccata taccgctatt aattaccgta actacatgtt tcttttacta gcgtttcttt
     1741 ataggatctt tcataatctc atttatagta acattaagaa tcccttattc tactatagtc
     1801 acttccacat caatattgcc tcttgttgct ttagagtatg ggcaaaatgc gtgggcttct
     1861 tctgcgattt tctctgcttg gtccttctca agtcctggca aagaaacttc taaaacgacc
     1921 ccgatttggt agcccccatc ttctaattga aataaagata cccgtgcttt gacttcagaa
     1981 tcagactcaa gattattttc ttccagcatg tgttgaaccg cgccgttgaa gcaagatgcg
     2041 tatccagccg caaaaagttg ttccggattt gtcccttcac tatggattcc tgggcggtcg
     2101 atcttcatgt gcatacttcc gtttggtgcc tcaacttcgc catcacggcc tccacgattg
     2161 atcattgctg tttcgtacat ttttttcatt gagatcatcc tttctttcgt cgttctatat
     2221 taagtgtaac gagtattttt taatctgaca aaggtaatgc ttgcattcgg cgacatcagt
     2281 tagacaacat cgatgatcag tgatagaagc agtttatcta tacaaaaaaa gaagggttct
     2341 atgatatttg gtgtccttac tcgccttgag taacaacacc aaatatcata gaaccactta
     2401 atcagcttca cactgctttt tctactttcg tagttgaaaa aaacttaaac aaatcatcgg
     2461 caaatagtta aagaaaaaac ttaatcgttg aaagaagccc aattgcttaa agaaggcaaa
     2521 ctgactaatc gacggaatcc gtgctaatcc gtaaagacct gcaattagta aactaataac
     2581 tgttaaaaca agataaaaat ggctaagcgt accttgacca ctttgacgat aaagcagaac
     2641 aagtaacaat gggaaaagta gaaagcctgc atagcctagc ccagaaccta tattatggat
     2701 ccaagtcgat agattacaag agctttcatt cgtattgata ctgaataagc cagtaaaaat
     2761 acaatcacct atcccatata aaccaactgt tgcaacaaca agtattgcaa gtgtttttga
     2821 tgttccaaca aataggtgat acaacgcagg caacgaaaga acaaaaaaaa gtccagatac
     2881 aactgaccag accaagaaag cacgtctgac tggactatct acgtcaccaa acacactaat
     2941 tacggtagtc atttgattca gtttgggata aaaaaggcca aggatatacg gtgtcgataa
     3001 atcactcatc acacctaata ataaaaaata aaaaccatat ttttgcaaaa aaatcatttt
     3061 cttcccctac tttttagtga ttattcgata catccaacaa tcaaattttt tc
//
LOCUS       DADJYG010000207         3074 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.214, whole genome shotgun sequence.
ACCESSION   DADJYG010000207 DADJYG010000000
VERSION     DADJYG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.214"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..473
                     /locus_tag="JZ382_002590"
     CDS             177..473
                     /locus_tag="JZ382_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428727.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="JZ382_002591"
     CDS             480..2432
                     /locus_tag="JZ382_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAY2428728.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="JZ382_002592"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="JZ382_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAY2428729.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1086 a          511 c          545 g          932 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcc
//
LOCUS       DADJYG010000208         3059 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.215, whole genome shotgun sequence.
ACCESSION   DADJYG010000208 DADJYG010000000
VERSION     DADJYG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.215"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..132)
                     /locus_tag="JZ382_002593"
     CDS             complement(<1..132)
                     /locus_tag="JZ382_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY2428730.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..2914
                     /locus_tag="JZ382_002594"
     CDS             542..2914
                     /locus_tag="JZ382_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428731.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1205 a          494 c          653 g          707 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacgga
      541 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
      601 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      661 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      721 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      781 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      841 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      901 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      961 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
     1021 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
     1081 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
     1141 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
     1201 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
     1261 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
     1321 tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata aattctattt
     1381 tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt
     1441 caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgaaaa
     1501 ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga
     1561 aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta agcaagaagt
     1621 caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgaaga
     1681 cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta gaaatcaatt
     1741 attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg cgttgaaaaa
     1801 agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt
     1861 caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga
     1921 taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga
     1981 aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc
     2041 gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat
     2101 tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta
     2161 tcaaagagaa ttaggacaag cacggggggc atatgttcgt aacgaattta ctggcgaaga
     2221 agaaatcgtc atgaatactc gaaatactcc tacgcagcat ttagctacaa gtattcatga
     2281 attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca
     2341 ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt cggagaaagc
     2401 tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagctgc
     2461 ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat
     2521 ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa
     2581 gcaggagcat aaaaatactg tagaaacggc agaacaaaag aatattgttc ctgttggttc
     2641 actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca
     2701 tttgaaggat gcggcgttta ttgaagatcc acagcataat caagtaattt taaaaggaaa
     2761 aagttatgga aaagagatcc aaacggcgtt accttcagaa gaatttgcgc taggaaattc
     2821 attccgattt tcacttgaga cagtgtcaga agtggcaaaa cgaaaaaatg aaatctatta
     2881 tgaacaacaa ttgtcacaaa aagaacgtgt ataaaacaag taaaaaagac tatctgaaaa
     2941 acggatagtc ttttttcttt tgggaggttt taaaatgacc gagaaaaaaa tgagtcttat
     3001 tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtag
//
LOCUS       DADJYG010000209         3022 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.216, whole genome shotgun sequence.
ACCESSION   DADJYG010000209 DADJYG010000000
VERSION     DADJYG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.216"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..296
                     /locus_tag="JZ382_002595"
     CDS             <1..296
                     /locus_tag="JZ382_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2428732.1"
                     /translation="DCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(382..1476)
                     /locus_tag="JZ382_002596"
     CDS             complement(382..1476)
                     /locus_tag="JZ382_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAY2428733.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1665..2426
                     /locus_tag="JZ382_002597"
     CDS             1665..2426
                     /locus_tag="JZ382_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY2428734.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2541..2981)
                     /gene="fabZ"
                     /locus_tag="JZ382_002598"
     CDS             complement(2541..2981)
                     /gene="fabZ"
                     /locus_tag="JZ382_002598"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY2428735.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          968 a          600 c          513 g          941 t
ORIGIN      
        1 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
       61 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
      121 ttctgcgtcg taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta
      181 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
      241 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
      301 tcataaccta taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt
      361 atttctgcaa taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg
      421 tttcaaaaag tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg
      481 tccgtcgttt ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc
      541 ttcattgtca atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc
      601 tttatctttg tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc
      661 tcgataacta tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc
      721 cgtatctact ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc
      781 ttcttcactt gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa
      841 atgcttcact tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc
      901 ttgcgtaggc caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa
      961 attatatttt ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta
     1021 tcgatgagga taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat
     1081 gatgttgtca acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg
     1141 attgcgcgtc atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta
     1201 ggcacccatc ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa
     1261 cgctggattg atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc
     1321 tttaaattct gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac
     1381 ccgtttatac ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga
     1441 ccgttctgga taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt
     1501 tcgtttttgt atccacttac attatagcaa acacaaagta aatggtctac accttttctt
     1561 tttcttttaa aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg
     1621 ttatcattaa agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag
     1681 ttattttaag tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca
     1741 acacgacttc ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca
     1801 cgactgccag tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg
     1861 ccgaaaatga agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat
     1921 ttcccgaaca cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact
     1981 acaacggact gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta
     2041 aaggcgatgg cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca
     2101 accaccttta taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta
     2161 acggcagcca gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag
     2221 acgactatcc tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg
     2281 gccattatac tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa
     2341 atatcgaaac agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata
     2401 tactgcaaga agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac
     2461 gtatgctagg attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt
     2521 tgctatatca ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca
     2581 cagacttttt tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt
     2641 ttggtgattt caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg
     2701 gccttattga tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata
     2761 agtatcgaac ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg
     2821 tcaggaaagt gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc
     2881 ttttttcctg gttctaaagt atctacataa tcaatataac aaataggata acgattagga
     2941 atcaattcca ttacttcagt agcagataat acgtaagaca taattaaact ccttctttca
     3001 ggcaattgcc attttcatta ct
//
LOCUS       DADJYG010000210         3013 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.217, whole genome shotgun sequence.
ACCESSION   DADJYG010000210 DADJYG010000000
VERSION     DADJYG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.217"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..359
                     /locus_tag="JZ382_002599"
     CDS             177..359
                     /locus_tag="JZ382_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAY2428736.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="JZ382_002600"
     CDS             462..746
                     /locus_tag="JZ382_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428737.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="JZ382_002601"
     CDS             762..998
                     /locus_tag="JZ382_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428738.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="JZ382_002602"
     CDS             1020..1274
                     /locus_tag="JZ382_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428739.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="JZ382_002603"
     CDS             1356..1541
                     /locus_tag="JZ382_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY2428740.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="JZ382_002604"
     CDS             1608..2003
                     /locus_tag="JZ382_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAY2428741.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="JZ382_002605"
     CDS             1993..2370
                     /locus_tag="JZ382_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428742.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="JZ382_002606"
     CDS             2371..2766
                     /locus_tag="JZ382_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428743.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DADJYG010000211         3012 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.218, whole genome shotgun sequence.
ACCESSION   DADJYG010000211 DADJYG010000000
VERSION     DADJYG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.218"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..750
                     /locus_tag="JZ382_002607"
     CDS             <1..750
                     /locus_tag="JZ382_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2428744.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
     gene            2301..3012
                     /locus_tag="JZ382_002608"
                     /pseudo
     CDS             2301..3012
                     /locus_tag="JZ382_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1085 a          437 c          592 g          898 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     2341 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     2401 ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact gctcttgatt
     2461 attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg
     2521 ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta
     2581 tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac actttatcgt ttattttcta
     2641 aaggaatatt tgatattgac acactaccga tgaaaggtaa aagaaaaccc aatggccatc
     2701 aggaaaaacg gggaaaacaa cagtatcagc gctcaatcca tgatcgacct gataattatc
     2761 ctcatttcaa ttctgagttt ggtcaccttg aaggtgatac gatcgttggc attcatcata
     2821 aaagtgccgt cattacttta gttgaaagat tatctaaagt cattatcacg attaaaccca
     2881 acggccgtaa ggcattagat attgaaactg cccttaatca atggttttct cgcttcccta
     2941 aaaacttctt taaatctatt acgctattac gtttgactgt ggaaaagaat tttctaactg
     3001 gaaagccatt ag
//
LOCUS       DADJYG010000212         2947 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.220, whole genome shotgun sequence.
ACCESSION   DADJYG010000212 DADJYG010000000
VERSION     DADJYG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.220"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(449..865)
                     /locus_tag="JZ382_002609"
     CDS             complement(449..865)
                     /locus_tag="JZ382_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428745.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="JZ382_002610"
     CDS             complement(867..1148)
                     /locus_tag="JZ382_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428746.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="JZ382_002611"
     CDS             1948..2922
                     /locus_tag="JZ382_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAY2428747.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DADJYG010000213         2945 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.221, whole genome shotgun sequence.
ACCESSION   DADJYG010000213 DADJYG010000000
VERSION     DADJYG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.221"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            136..1083
                     /locus_tag="JZ382_002612"
     CDS             136..1083
                     /locus_tag="JZ382_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY2428748.1"
                     /translation="MTDRISLEKEAIAFSDANAPHPRIYELAPEEGRALLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKINIRFVRPEGNIDKLPVIFYIHGAGWVFGSTQTHDKLIR
                     ELSVRTNSVVVFPEYSRSPEAKYPIAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMRWFWD
                     QYTTNEEERAEITASPLRAELNELKDLPPAMILTGEADVLREEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIMEKE"
     gene            1219..1848
                     /gene="upp"
                     /locus_tag="JZ382_002613"
     CDS             1219..1848
                     /gene="upp"
                     /locus_tag="JZ382_002613"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY2428749.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETFEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(1986..2228)
                     /locus_tag="JZ382_002614"
     CDS             complement(1986..2228)
                     /locus_tag="JZ382_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428750.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKPQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            2349..2421
                     /locus_tag="JZ382_002615"
     tRNA            2349..2421
                     /locus_tag="JZ382_002615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Thr,seq:cgt)
     gene            2560..2859
                     /locus_tag="JZ382_002616"
     CDS             2560..2859
                     /locus_tag="JZ382_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAY2428751.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEINEKERILLIEAKTGLEKGQYY
                     PKVMNNLERSLRPLAIKGELSKAVSPFYMEISTIGKFEKELGRGMASASITFGHL"
BASE COUNT          963 a          493 c          618 g          871 t
ORIGIN      
        1 ttcaataccg gacttttctg tttttttcta ttttattttt ttatggaaaa cggattcttt
       61 gacaatattg atgatgctct ctagtatatg aggtgtaatt ttagtcttca cattaaatct
      121 cagggaggaa aagtcatgac tgatcgtatc tcgttagaaa aggaagccat agcattcagt
      181 gatgcaaatg cacctcatcc gcgaatctat gaactagcac cggaagaagg aagagcatta
      241 ctggaagaag tacaagaatc acctgtgaac aaatattccg ttgatattga agacttgatg
      301 gtagatactg gcgaatgggg aaaaatcaat attcgttttg tacgaccgga aggaaatata
      361 gataaattac cagttatttt ttatatccat ggagctggtt gggtctttgg cagtactcaa
      421 acccatgata aattgatcag agaactttcg gtccgcacga attctgtcgt tgtctttcca
      481 gaatatagtc gatcacctga agcgaaatat ccaatagcaa ttgaacaaaa ttatgctgtg
      541 ttgcaacagc tgaataaatt agccaagtca aaaaatctgg atctcgagaa tctaatcgtt
      601 gcaggagatt cagttggtgg taacatggct acagtcatga cgatcttaac aaaagaacga
      661 aaagggctcc caatatctaa acaattgctg tattatccag ttaccaacgc tgattttgat
      721 acagattcct atcatgaatt cggagaaaac tattttttga caagagaagg aatgagatgg
      781 ttttgggatc aatacacgac gaatgaggaa gagcgtgctg agatcacagc ttcgcctttg
      841 agagcagaac tgaatgaact aaaagatcta cctccggcaa tgattttgac aggagaagcg
      901 gatgtattaa gagaggaagg cgaggcttat gcacgcaaac ttcgtgatgc aggggttcct
      961 gttacacaag tacgcttcca aggaatcatc catgatttcg tcatggtaaa tgctatggat
     1021 cagacaaagg caacgcgtgc agcaatggca ttatctactt cttggatcat ggaaaaagaa
     1081 taagctttgc ttgtcagcag gaaaacttct tttcaaaaaa ggattttctt aggaaaaatg
     1141 gcgagtttga ctagtcaatc acttgatttt gttttacaat taaaaagaaa cgacagaaca
     1201 aaggagagct taaaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac
     1261 aaactaacta ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat
     1321 gaaatcgcta tgttgatggc atatgaagta tcacgggata tgccgttgga agacatcgtg
     1381 atcgaaacac cgatcactga aacaacacaa aaaacgttgt ccggtaaaaa agtagcgatt
     1441 attccaatct tgcgtgcagg tatcggaatg gttgatggta tcttggaatt gattccagct
     1501 gctaaagtgg gccatatcgg tctttatcga gatgaagaaa cgtttgagcc acatgaatat
     1561 ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt tcgtagtaga tccaatgttg
     1621 gcaacaggtg gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac
     1681 atcaaatttg tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat
     1741 cctgatattg atatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc
     1801 gttcctggac taggggatgc gggtgaccgt ttgtttggta caaaataagt aaaatgaaaa
     1861 aggatgtgac acaacttttg tcacatcctt ttttggtcat ttttaatgtt tgaggcagag
     1921 cagctttttc acagctttcg tttgtatata aaaaaaccat cccaaaaatg cagagatggt
     1981 caaattcaaa tctttttaag ttctttgtag ttaacgatta ctacaccttt gctttgcgtc
     2041 atcaagttcg cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgt
     2101 tttgaaatcg ttccagtttg cggcttgccg tgaatcgtga acattacgtg agtattgatc
     2161 ttgaattttt caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc
     2221 attttcataa aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa
     2281 attaaactaa ttttccatcg aactctttac aatcagccaa atagagtata taatttcttt
     2341 tggataaggc cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag
     2401 gttcgatccc tatagctggc ataaagcaaa agacagatac cctggttggg tatctgtctt
     2461 ttttctttat gtctgtgtga tttaaaaacc actaaaaatg gcgtatactt tgtgtataga
     2521 atgtagtggg gagaaatata tttctgggag gaaatcgtga tgtctaaaag tgaagaaata
     2581 gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa tgaaaaggaa
     2641 cgtatacttc tgatagaagc aaaaacaggc ttagaaaaag gtcagtatta tcctaaagtc
     2701 atgaataatt tggaacgtag tttacgtccg ttagcaatca agggagaatt gtctaaagca
     2761 gtctcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga attaggtcga
     2821 ggaatggctt cggcatcgat tacttttggg catttataaa aatggattat tcatctcaat
     2881 gaattaaaaa gtagcgtcaa tcacagaata caggctaggt tcgtttattt gaacaaagtc
     2941 tgtat
//
LOCUS       DADJYG010000214         2914 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.222, whole genome shotgun sequence.
ACCESSION   DADJYG010000214 DADJYG010000000
VERSION     DADJYG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.222"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            588..>2914
                     /gene="esp"
                     /locus_tag="JZ382_002617"
     CDS             588..>2914
                     /gene="esp"
                     /locus_tag="JZ382_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAY2428752.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1061 a          443 c          569 g          841 t
ORIGIN      
        1 atatggacaa ttcaattata aagaaaaaga atcaataatt taatgaggct gtgacaaatg
       61 tcattttttg tttcgctaaa aacgagtgaa aaaatgttga taactccaat cattcgttta
      121 agattctagg aatcattcag aaatgctgtg aaaccgatac tttataatta taaagaaggt
      181 gatttcaaat tccgaagaag gaatttgaag tcaccttttt acttatgtca cagtctcatt
      241 cagtaatgcg ggataaggct ccttattccg ttatttgaat actctgaagt ttaatctaag
      301 aaagggtaat attttgaaaa catgcttcat cgcatagaaa tgaacttaat ttcagaaaga
      361 atacatacat atctttttat atgttttcag ctgctgaatg gttaaaaagc acaaatatcc
      421 gaaaaaaaca tgaagaaatg tacttttttt gttgtaatgt tcatgaaatt ctctaagtaa
      481 attagttaac ataaggttag taagattcct attctgtgaa taatacgtat cagatttatc
      541 ttttattctt actaggaaag taattattaa gggagttgat ttgaataatg gttagcaaga
      601 ataataagag agtatttcta gaaaaaacaa agaaacgagt attgaagtac agtatcaaaa
      661 aattaagtgt tggggttgca tctgttttga ttggtgttgg tcttgtcttg ggaacaactg
      721 aactagttaa agcacaagat gaaatttctc caagcagccc acttgaaaca gcgataagct
      781 ccgtacaagt aggtgacaaa gtagcttcgg ggaatacttt tcaggagaat cctggttata
      841 cgaaaaatta taacttttcg gatttacaat ttagtcctca agaactaact ggagacacac
      901 taaaaggcaa tacaattgga tttgaagttt acggaaaaca taatattgca gcatcaacta
      961 aaaactggga aatccgtctt caattagatg aacgattagc taaatatgta gaaaaaattc
     1021 aggtcgatcc aaagaaagga attggatctt ctagacggac ctttgtaaga attaatgatt
     1081 cgctgggtag acctacaaat atttggaagg tcaattatat tcgtgcaagt gatggattat
     1141 ttgctggggc agaaacaacc gatacgcaaa ccgctcctaa tggggtaatt acgtttgaaa
     1201 agagcctaga tgaaattttt aaagagatag gcatagacaa tctcaagacc gatcgtttaa
     1261 tgtatcgaat ctacttggta agtcatcaag acgatgataa aattgtaccg ggtattgata
     1321 gtacgggcta ctttttaaca gattcagatg atttctataa tagcttagat gtatctgaaa
     1381 ataatcctga ccagtttaaa catggatcag taagtgcaaa atatgagaaa cctaatactc
     1441 aaacaaaaga tggttcagga tccacaggtg ctaatggagc tataatctta gatcataagt
     1501 taacgaaaaa ttataatttc tcatattcat cttccgcaaa gggcacgcct tggtatgcta
     1561 attacaaaat tgatgaacga ttagttccat atgtagcggg tatacagatg cacatggttc
     1621 aggctgacaa agtaacatat gatgtttcgt ttgaatccgg aacaaaagta gctgatttag
     1681 caatcgagcg acgggaaggt catgaaaatt atggcatagg ttcaataact aataatgatt
     1741 taactaaact tatcgatttt gctaatgcaa gtccacgtcc agtcgttatt cgatatgttt
     1801 tacaattgac taagccatta gatgaaatct tagaagatat gaaagcaaca gcacaagttg
     1861 aagaaaataa accatttggt gaagatttca tctttgattc ttggttgtcg gatacgaata
     1921 aaaaattaat ccagaacact tatggaacag gttattatta tttgcaagat attgatggtg
     1981 atggaaaccc tgacgataaa gaagagagcg gagacacgaa tccatatatc gggaaacctg
     2041 aattagaaga agtatatgat gttgacacaa cagttaaggg gaaagtattc atccacgagt
     2101 tagcgggaac aggtcacaaa gcccaacttg ttgataaaga aggtactgta ttagcagaaa
     2161 aaactatcgc tccaaatgaa aaagatgggg ctccaatttc agatactgta gaatttgaat
     2221 ttacgggtgt agattcaagt aaattaatcg cgaaagatga attaaaaatc caaatcgttt
     2281 ctccaggttt tgataaacca gaagaaggtt caaccgttat taaggaatca ccaaaagcgg
     2341 ttgataaaca aaccgtggta gttggattta aaccagatgc taaagaatca attcggaata
     2401 ataaaaactt acctgaagat gcagagtatt catggaaaac agagcctgat acttctaacg
     2461 ttactgatag tacgaaaggt attgtaactg ttaagatcgg aaatcgaact ttcgacgtgg
     2521 atgtagagtt tgctgtaaaa gcttctcaag ctatggaaaa tgatgcaaca tacgtaccta
     2581 taacaacaac tccagaaacg acagttcaaa gtggaaaacc tacatttgat aaaccagatg
     2641 ttcctttagc taaagatgcc ttttcaattt cagatgtcta taataagtac ttcggtaatg
     2701 caagtgttga tgcaaacact ggtattgtta cattcactcc agctaaaggt gtaggagaat
     2761 cggagccgat tactggaaca attcctatta aaattgttta ccaagatggt tctgtaggca
     2821 cgaccgattt agcagtaact gtaagtaaag atatttatga aaatccagga gaaaacattc
     2881 ctgcaggcta ccacaaagta accttcaccg cagg
//
LOCUS       DADJYG010000215         2910 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.223, whole genome shotgun sequence.
ACCESSION   DADJYG010000215 DADJYG010000000
VERSION     DADJYG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.223"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(13..351)
                     /locus_tag="JZ382_002618"
     CDS             complement(13..351)
                     /locus_tag="JZ382_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAY2428753.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(367..570)
                     /locus_tag="JZ382_002619"
     CDS             complement(367..570)
                     /locus_tag="JZ382_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428754.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(585..1361)
                     /locus_tag="JZ382_002620"
     CDS             complement(585..1361)
                     /locus_tag="JZ382_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAY2428755.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1660..1980
                     /locus_tag="JZ382_002621"
     CDS             1660..1980
                     /locus_tag="JZ382_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428756.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1998..2429
                     /locus_tag="JZ382_002622"
     CDS             1998..2429
                     /locus_tag="JZ382_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAY2428757.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            2558..>2910
                     /locus_tag="JZ382_002623"
     CDS             2558..>2910
                     /locus_tag="JZ382_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAY2428758.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKET"
BASE COUNT          945 a          476 c          455 g         1034 t
ORIGIN      
        1 actagcgctc tattaaattt tcccatccgc ttgcctcctt aagatttccg aaaaatttgt
       61 ttctaaaaat tcaagtgttt tacttcttaa aaatagatat gtgtctcttc cttcaactgg
      121 ataatagaca aatccatttt tgtttttctc gatatcgata atatttctat atcttgggtt
      181 ctttaaaact ctagaagtaa accaatcata ttttctgtta atccgttcta gcacttctgg
      241 caacgtcatc catctaccag tatcatcagc tttttttaac tcctcataat ccacttggga
      301 gataattaca tagccttctg gaattgggat ttttgcttct agatattgca ttagctgttc
      361 ctccctttac aattcgtaca tagtgataat cgaatctata attctgtttg cttctgcaga
      421 agtcttttta ccgtttaaaa ttaaagataa gtagcttttg ctaattccaa atctttcagc
      481 aagcatggtg taagttaaga actttgaact ttcgacatat tctttgattt tttctctatc
      541 tcgttgagtg atttctgcaa tgtcagtcat actaaaactc ctttctaacc aatttcctct
      601 aaatccattt gagggtaata tccttctttt tttagtaatt cgtagataaa tagacgccct
      661 ttctgtgtcc atttggtatt cattacaatt ttagtgccac catcggcttt cgggatctca
      721 gttgtatgag attttgtgta tccttggttc atgtgttttt tgcacaataa ccattggtta
      781 ccgacttttt tctgaatacc tagtttatga agtaatttat tcatctgttg tggagacatc
      841 ccataatctg ctgcaatctg actaattgtt actgaatctg tagaagataa tatgctatct
      901 aaataggaga ttttaggttc gtactctgca atcttttgtt ctgcgattaa tcttccagtt
      961 ctttcttctt ttagttgagt tgctaattga atgattgtat ctggattaag caaagcttct
     1021 tctacttttt ctggagttag ataacctcca tgttttctaa ttgctggcaa cacttcactt
     1081 gttacccaac gtttaaattt tttggcagag ggaagttttg attttaagat taaactgtac
     1141 aaaccagatt cgttgatgat tgtcatctct cttgattgac ctgaggtcgt gattcgcgac
     1201 cccatcttat cttccaaatc tacatgccgc gacaaagcat ctttagtgtt tgaataaccc
     1261 aaaacactcg caacgtcttt gcctacaaaa tatggttcat catttactaa aattgtccga
     1321 acttcgtttt gttcgaaatt aaaaatttgc ggtgtgttca tatttctcat tcctttcttt
     1381 tatattcgta aacaaattta acaacttttt tctaaattca attgacagta tctgagtaat
     1441 actctatact aagagcataa ttaaataaga cataaaaaca ttgattttaa agctttcttg
     1501 gcggtctgca tttacatatc aatagtgttt tttgttgtct ttttagttgt taaatctgtt
     1561 tacaagatat agtatagctc attactcaga attagtcaaa ttatttctgc gtatttttct
     1621 aaactttttt tgtaaacatt tagaaaggtt gttttatcga tgaataccta tgaaataata
     1681 aaagacttgg cgaagaggaa aaagatatct atacggcagt tagaaattaa ttttggatat
     1741 tctaacggtt atctagcaaa gtggaaaaat aatactccta attcagatga attaagtcgg
     1801 attgctgact atttcgatgt ctctgtagac taccttctag gtagagaaga aagagaaacc
     1861 cctaaacatg tggatttatc agaagacgat actgtttttt cttttgatgg aaaagaaata
     1921 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     1981 ttggagtgat ggtttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     2041 aagtagttta tatggagatg aaagagcctg gtttctacta tccaaaaccc agaatcattt
     2101 ttttgaatga aaatctatac ggtgaaactg cagaagcctt tcatttatct catgaacttg
     2161 cacatttcag cgcctctcat tttgaatttt cagttttgta cgatacttct acaacttttc
     2221 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     2281 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     2341 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     2401 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     2461 cagcaaaaca ttaaaaaagc ccgtgctgca acacgggctc tttcctcata tatgagcttc
     2521 tacaaaaaaa tcatatcaca gaaatgagga agaaaaaatg gaaaaagaga ttttaataaa
     2581 atctccagga aaaaccttga ttaaagtaac tgatgattct atttcaatta ttagaaaagg
     2641 atttataaat ttagttaatc aaggtattaa aggagaaaaa actatcccct ttaaaaatat
     2701 atcagccgta caattaaaaa aaccaggtat gagtaatggt tatatacaat ttaccttgct
     2761 aggcggaaac gaaagtcgcg gaggtatatt agcggccacg aaagatgaaa acaccgttat
     2821 gtttacaaaa aaatattgga atgaaatgga aaatttaaaa aaatatatag agaagcaaca
     2881 atctatatta gataataatt ccaaagaaac
//
LOCUS       DADJYG010000216         2870 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.224, whole genome shotgun sequence.
ACCESSION   DADJYG010000216 DADJYG010000000
VERSION     DADJYG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.224"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..718
                     /locus_tag="JZ382_002624"
     CDS             <1..718
                     /locus_tag="JZ382_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAY2428759.1"
                     /translation="INPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIA
                     SPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSND
                     ARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNV
                     RAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLE
                     EDENVISLAEIAFPNEKIFR"
     gene            734..1774
                     /locus_tag="JZ382_002625"
     CDS             734..1774
                     /locus_tag="JZ382_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY2428760.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1793..>2870
                     /locus_tag="JZ382_002626"
     CDS             1793..>2870
                     /locus_tag="JZ382_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAY2428761.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSR"
BASE COUNT         1044 a          416 c          546 g          864 t
ORIGIN      
        1 gatcaatcca aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag
       61 aatccaacct gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt
      121 attagttatt gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc
      181 gggaccaatc atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta
      241 taaattccgc tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag
      301 caatgatgca cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt
      361 accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg
      421 ttctttcttc gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt
      481 tcgtgctggt atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag
      541 taaattacgt tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat
      601 cttgctgcaa accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga
      661 cgaaaatgtg atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt
      721 gggaggcgta aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga
      781 agcaactatt ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt
      841 cgttttagta aatgatggat caacagatca aacacaggaa atcattcaag aactgattga
      901 aaatgatgaa cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa
      961 cgaaggaata agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc
     1021 agtaaaaacg atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct
     1081 tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga
     1141 aaacttagtt acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca
     1201 gttaatgaat gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct
     1261 gaaaaataac caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt
     1321 acattattat gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga
     1381 atttgaaggt gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa
     1441 agaattctat tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc
     1501 gcttctttta aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac
     1561 ttcaaaagag aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc
     1621 caagcaaatt gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat
     1681 gccagctcca ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc
     1741 agggttaatg gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag
     1801 aattttgcat tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt
     1861 atatcgtaaa attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc
     1921 agatgttcaa gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc
     1981 taatcctaag aaaaatattt ttaaatattt aatagaagtg aataagttac taaaaaaaga
     2041 atctttttct atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc
     2101 caaaaagaat ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc
     2161 tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc
     2221 aacagactac cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accatccctt
     2281 tgagatagtt cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt
     2341 taataagaga aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg
     2401 attagagaaa caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat
     2461 gaattctgat tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc
     2521 caaagcagta gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat
     2581 tcccgagtta ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc
     2641 aatggttgga ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc
     2701 caaagataca aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga
     2761 atgggcaaaa aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag
     2821 ttatgatgtt tcgtacacta ctgaacaaat ggagagtata tatagtagag
//
LOCUS       DADJYG010000217         2865 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.225, whole genome shotgun sequence.
ACCESSION   DADJYG010000217 DADJYG010000000
VERSION     DADJYG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.225"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1932
                     /locus_tag="JZ382_002627"
     CDS             <1..1932
                     /locus_tag="JZ382_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY2428762.1"
                     /translation="VVKQQLQSADTIIIATDSDREGELIAWTIIQQAGADQGKTFKRL
                     WINSLEKEAIYQGFQQLRDAEETYPKFEEAQARQIADWLIGMNGSPLYSLLLQQKGIP
                     GSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQ
                     EAFEAYLKEKGVQVGKQPGTILEVETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTL
                     EAMQGLYEGKYLSYPRTDTPYITEGEHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYV
                     NNKKVQEHYAIIPTKTVMTEATYEKLTPLQQAIYDQVLKTTVAMFAEKYTYEETTILT
                     QVQQLQLKAVGKVPVDLGWKKLFGKESDRKEKEEEPLLPKVTKGETVTVDLQIFEKET
                     KPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANIIETLKQKEYIKV
                     EKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLTKIGEQKGTQATFLANIQKFV
                     SHLLEVVPGQIQATDFGATLQEVKVASEKQEAARHLGSCPKCQEKEVILYQKVATCTS
                     EACDFKLWVTIAKKKLTATQLKEIIQNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGF
                     EFSEKKKTTYKRKVRRKTS"
     gene            2178..2771
                     /locus_tag="JZ382_002628"
     CDS             2178..2771
                     /locus_tag="JZ382_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY2428763.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
BASE COUNT         1095 a          510 c          605 g          655 t
ORIGIN      
        1 gtggtgaaac agcaacttca atcggccgac accattatta ttgcgacaga tagcgatcgt
       61 gaaggggaac tgatcgcttg gacgatcatt caacaagcag gcgctgatca aggaaaaacc
      121 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccagcaa
      181 ttacgagacg cagaagagac ctatcccaaa tttgaagaag cacaagcacg ccagatcgct
      241 gattggttga tcggcatgaa cggcagccca ctgtatagtt tactcttgca gcaaaagggc
      301 ataccaggaa gtttttcctt aggcagagtc caaaccccaa cgctttacat gatttatcag
      361 ctgcaggaaa agattagaaa cttccaaaaa gagccttatt ttgaagggaa agcccaagta
      421 accgctcaaa atggcatatt tgatgcgaag cttgatccca atgaaaccca agcgacgcaa
      481 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaag taggaaagca gccaggcacg
      541 attcttgaag tcgaaaccga gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc
      601 agccttcagt ctaaaatgaa tcagctcatg aaagctagtg ccaaagatac tttggaagcc
      661 atgcaagggt tgtatgaagg gaaatatcta agctatccac gaacagacac cccttatatt
      721 acagaaggag agcatgcgta tctgttggat catttagatg aatacaagca ttttttaaag
      781 gctgaatcat tccctactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
      841 gtccaggaac attacgcgat cattcctaca aaaacagtta tgacagaagc aacgtatgaa
      901 aagctgacac ctttacaaca agcgatttat gatcaagttt taaagacgac ggtggccatg
      961 tttgcggaaa aatataccta tgaagaaact acgattttga cacaagtcca gcagcttcag
     1021 ctaaaggcag tcggaaaagt accagtagat ctaggttgga aaaagctatt tggcaaagag
     1081 agcgacagaa aagagaagga agaagaaccg ctacttccta aagtaacaaa aggagaaacg
     1141 gtgacggtag atctacaaat ctttgaaaaa gaaaccaagc caccacaacc ttacacggaa
     1201 ggtacactaa tcaccgccat gaagacagct ggtaaaaccg tggatagtga agaagcacaa
     1261 tccatattga aagaagtgga gggaattggt acagaagcga cacgagcaaa tattattgaa
     1321 accttgaagc aaaaggaata tatcaaggta gagaaaaata aacttgtggt gacgaataaa
     1381 ggaattttgc tttgtcaggc tgttgaaaaa gaaccgctgt taacaagtgc cgaaatgacc
     1441 gccaaatggg aaagctatct tacaaagatt ggtgagcaaa aagggacaca agctactttt
     1501 ctagcaaata tccaaaaatt tgtttcccac ttgttggaag tcgtcccagg acaaatccaa
     1561 gccactgatt ttggagcaac gttacaagag gtgaaagtag ccagtgaaaa gcaagaagcg
     1621 gcgcgtcatt taggatcttg tccaaagtgc caggaaaaag aagtcatact ttatcagaag
     1681 gtggcgactt gcacgtctga agcctgtgat tttaaattgt gggtaacgat tgccaaaaag
     1741 aaactcacag cgacacaact gaaagagatc attcagaacg gccgaacaag tcaaccagtc
     1801 agaggactaa aaggtcagaa aggttcgttt gaagcaacga tcgttttaaa agaagacttt
     1861 acaacaggat ttgagttttc tgaaaagaag aagaccacct acaaaagaaa agtgagacgt
     1921 aaaacgagtt agaattgacg gagggaaaaa cgatgagaaa tacaacgcat aaagaacaag
     1981 aaatcgaaaa tcaaaaagcc aaaatacgtt cagagcagga gaaaatgcgt gccagcaaac
     2041 gaagactaca gcaattgaag aatcaacaac ggattcaaaa agaacttgaa ttagatcgat
     2101 aaatcctcct gttgttaata ttaaactaac atgttatagt tatgttagtt taatattaac
     2161 attcgagagg gaaagcgatg aaagataaag tcaagtgcat ttttgagaaa tacaatggta
     2221 atctatcctt gaaggatgct agaaatgaag ggttatcacc tatgacgtta gatcgtttag
     2281 ccgaaaagga caaaatagaa aaaatagcat caggttttta taccttaaaa gatagttttg
     2341 tggatgaatt atatctcgct caaagtatct atactagagg aatcttctct cacgagacag
     2401 cgcttgatct ttttcaagta ggaacatata tccctaaaaa aattaatatg atgtttccta
     2461 aaggatacaa tgtttcgaaa gagaatttag atcagtatgc cattcaaccg cattacacga
     2521 ataaagaaaa ctttgaactg ggaatcacag aggcagaaag cttttacggc aataaaattt
     2581 tcgtctatga tttagagcga acgttatgtg atatgtggaa tcccagatat aaagcaagcg
     2641 tagaagtaaa acaagaagca ttaaaagaat atatgacaag taccagcaga aatacaaata
     2701 agctaagaag atacatgaat attttaaaat caccaaaaga aatgactttg tatatgttgc
     2761 cactttattg agaggagtaa aggtgaatga caccgacaca atttaaagcg aaaacaagaa
     2821 atgttgcagc tgagaaacat ttaaacccac aaattgttca aagat
//
LOCUS       DADJYG010000218         2860 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.226, whole genome shotgun sequence.
ACCESSION   DADJYG010000218 DADJYG010000000
VERSION     DADJYG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.226"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..499
                     /locus_tag="JZ382_002629"
     CDS             <1..499
                     /locus_tag="JZ382_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAY2428764.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCAN
                     ASEAV"
     gene            600..1172
                     /locus_tag="JZ382_002630"
     CDS             600..1172
                     /locus_tag="JZ382_002630"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY2428765.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGW
                     CYLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAR
                     EVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYI
                     EGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1528..2100)
                     /locus_tag="JZ382_002631"
     CDS             complement(1528..2100)
                     /locus_tag="JZ382_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428766.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2116..2721)
                     /locus_tag="JZ382_002632"
     CDS             complement(2116..2721)
                     /locus_tag="JZ382_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAY2428767.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          893 a          570 c          471 g          926 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg agttatttga
      361 ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg agacaccggt
      421 tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg gatgtgctaa
      481 cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc acatccgagg
      541 aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca ccttataaaa
      601 tttgtcaaaa aaactgttgc cattccatca tttcaaaaga gaatctctta aatcaggact
      661 tttctactga aacgatctgt gagaaatggg cagctgacat tacgtacatt cctactaaga
      721 aaaatggttg gtgttactta tcttcaatca tggatttaca cacgaaaaga attattagtt
      781 atacattttc aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa
      841 tacattatca cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag
      901 caagagaagt ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag
      961 gaacacctta tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag
     1021 tctacacgac tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg
     1081 aaggatttta taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat
     1141 ttgaagaatt agcaaaagaa aaaatggctt aaccgtctct actaaagaca atcaacaccg
     1201 tagggatatg gagataaaat caatagagtc aatagattat ttatcgataa taacactaaa
     1261 aaagccacgt tcctaaacgt gacttacgct gaacgatatg tgttgtttgg tagacgaaca
     1321 aatcgttcat tataaatata tgatggtatc ctaatatagt aagcgtggct agcatacctt
     1381 taattacttt accttttaca gttaccatac acattttttg agcagaaatc aagtaaataa
     1441 aaaaatatta tatttcacaa catgctaata gactcatatc tagaacgcat taagtattta
     1501 cctgtctggt tcgctgagac ttctttttta actttcttca attttcttct ttattctaaa
     1561 aagcgtactg cgactgaaac cggttatagc ttcaacttcc ttatacgatt tacctgaagt
     1621 aagcagttca tatgcgtgcc tcttcttcgg tgtgattgtt gctttcggac gtccttctct
     1681 gaaattagga ttattctttc ttgcaaacat ttttccttct tgggtacgtt ccacaatcat
     1741 atcacgttcc atttctgcta cactcaacaa cgtccgtgtc accattcgcc ccatggacgt
     1801 attttcaatc gttcctagat tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc
     1861 aatgatctct aatgcttctc tcgtgttacg agcaaatcga tcaagcttcg taacaactat
     1921 ttcatctcca ggcttcagac cagccaacag ctgattgaat tttggccgat cccgttttgt
     1981 acccgtaaat ttttctgaaa aaatattctt cttctctacg cagttctcaa gcaactgttg
     2041 aatttgttct tttgtacttt gagaagcggt tgagacacgt gcatatccat atttcgtcat
     2101 ttggtccacc tacttttaca aatcatctat cgtatactca gaataccctt catagaaata
     2161 gctgacatcg attcctttca tatataactc gcgatcgtca attttatcag tcagagcatt
     2221 ggaaattaga tagcgtattt ccaaatcgtt tatgggactt cgctccattg cagaaagata
     2281 gtctgcttta tcgattaagt tccaatctac aactttttgc aattcttcct ttaaaatgag
     2341 atccagccaa attctagtac ttcggccatt tccttctcga aatgggtgag cgatattcat
     2401 ctccacatat ttcttgataa tctcttcaaa agaagtttgt ggcatctgat caatatgctg
     2461 taacgaatgt tctaaataca taactggtgc aaatcgaaaa tttccttttg caatattgac
     2521 cgatcggatt tttccggcaa aatcgtaaat atccgaaaat agaaattcat gaatttcaga
     2581 taaaccttta aatgtaccaa cttcgatttc atttattttt ccacaatcgt acaattcttt
     2641 cgctttttgt ttactgagtt tttcttcaac cttagccaac tcaacttgat tggttaatcc
     2701 caatttattc tctaaaatca tgcgaaatct ccttaacttt atgacataag tttatgaaac
     2761 actctcaacc ccattatatc gcgattttgt ttatgtgtca aaaactatta agtttctgac
     2821 ccccgataga tttttcaagt gtctctattt tgcacagtct
//
LOCUS       DADJYG010000219         2857 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.227, whole genome shotgun sequence.
ACCESSION   DADJYG010000219 DADJYG010000000
VERSION     DADJYG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.227"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            562..843
                     /locus_tag="JZ382_002633"
     CDS             562..843
                     /locus_tag="JZ382_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428768.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="JZ382_002634"
     CDS             845..1261
                     /locus_tag="JZ382_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428769.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="JZ382_002635"
     CDS             complement(1422..1688)
                     /locus_tag="JZ382_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428770.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="JZ382_002636"
     CDS             complement(1646..2620)
                     /locus_tag="JZ382_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAY2428771.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DADJYG010000220         2831 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.228, whole genome shotgun sequence.
ACCESSION   DADJYG010000220 DADJYG010000000
VERSION     DADJYG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.228"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            274..2169
                     /gene="asnB"
                     /locus_tag="JZ382_002637"
     CDS             274..2169
                     /gene="asnB"
                     /locus_tag="JZ382_002637"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAY2428772.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2364..2810)
                     /locus_tag="JZ382_002638"
     CDS             complement(2364..2810)
                     /locus_tag="JZ382_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAY2428773.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          921 a          485 c          534 g          891 t
ORIGIN      
        1 acacgatctt taggtcgtgt ctttttttgc tggttttttt ggaaatagca tccactaata
       61 tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat
      121 tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt
      181 gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata
      241 aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac
      301 gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga
      361 cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag aagattaagt
      421 attattgact tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt
      481 atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc
      541 cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga
      601 acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag
      661 aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg
      721 aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac
      781 aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac
      841 ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa
      901 gatggaaaaa tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat
      961 tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat
     1021 acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta
     1081 acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat
     1141 gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg
     1201 atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct
     1261 gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg
     1321 cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga
     1381 ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa
     1441 ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaacttcca tggacggatc
     1501 catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc
     1561 gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat
     1621 gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg
     1681 caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt
     1741 tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg
     1801 gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga
     1861 caagctgcga acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct
     1921 gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc
     1981 gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac
     2041 catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca
     2101 tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg
     2161 actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg
     2221 agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg
     2281 gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt
     2341 cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt
     2401 ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct
     2461 tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat
     2521 tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc acgaggattc
     2581 gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat
     2641 tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta
     2701 tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat
     2761 ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat attagtatcc
     2821 ccctttatta a
//
LOCUS       DADJYG010000221         2830 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.229, whole genome shotgun sequence.
ACCESSION   DADJYG010000221 DADJYG010000000
VERSION     DADJYG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.229"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            802..1068
                     /locus_tag="JZ382_002639"
     CDS             802..1068
                     /locus_tag="JZ382_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428774.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="JZ382_002640"
     CDS             1126..1638
                     /locus_tag="JZ382_002640"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2428775.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="JZ382_002641"
     CDS             1635..2672
                     /locus_tag="JZ382_002641"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY2428776.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          747 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac
//
LOCUS       DADJYG010000222         2812 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.230, whole genome shotgun sequence.
ACCESSION   DADJYG010000222 DADJYG010000000
VERSION     DADJYG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.230"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            174..518
                     /locus_tag="JZ382_002642"
     CDS             174..518
                     /locus_tag="JZ382_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY2428777.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="JZ382_002643"
     CDS             519..938
                     /locus_tag="JZ382_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY2428778.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="JZ382_002644"
     CDS             complement(1030..1380)
                     /locus_tag="JZ382_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428779.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="JZ382_002645"
     CDS             complement(1346..2566)
                     /locus_tag="JZ382_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAY2428780.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT         1048 a          502 c          537 g          725 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gt
//
LOCUS       DADJYG010000223         2790 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.231, whole genome shotgun sequence.
ACCESSION   DADJYG010000223 DADJYG010000000
VERSION     DADJYG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.231"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            45..1463
                     /locus_tag="JZ382_002646"
     CDS             45..1463
                     /locus_tag="JZ382_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2428781.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKLGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKVAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            1475..2785
                     /locus_tag="JZ382_002647"
     CDS             1475..2785
                     /locus_tag="JZ382_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAY2428782.1"
                     /translation="MQTKKEFINALDFKTNDIQQDDTVMLQKAINQGAKEHSPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGKTEIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGEHWWELYWGKDQKSGTRAEYDRKGLRWIADYAIKRPRACLLYHA
                     EHVVIRDLIFQKSGFWNLQITYSNDVLVEKIIIRHNDGPSTDGIDIDSSTDVRIYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEIARCNIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSRERGGVIENIRVENLNMMDVQFPFSWIMDWHNEYNRKNSNDLE
                     RMPESWQAVAEEIPESKQMTKVRNIHVKNVQASLSPGYPLPSRAFDLVAFPEKTIEDV
                     CFTHCTITAKEFGRIEAVQNLCFESCILSIETGNTVANNTFDNR"
BASE COUNT          890 a          435 c          599 g          866 t
ORIGIN      
        1 tataattttg aaaacgttta ctataagagg taggaggaat tcaaatgctt tcacaaaatg
       61 ctgttacagc aaatacagta aaaaaagaaa aaaaggaatt tgggatttcc gataagttag
      121 gctacatgtt cggtgattta ggtaactgtt ttattttagg tttggtcaat agtttcttga
      181 tgatttatta tacgaatgtt cttggtgttt caggagcaat cgtaggtacg ttattcttga
      241 tttcaagaat tttggatgcg tttgctgatg tgatggttgg aagattatgc gatgtctcag
      301 agttaacaga aactggtcga tttattcctt ggatcagaaa aatgaaatat ccattttgct
      361 tagtgacagt cattttgttt ttaccattcg tccaacatct tccaatgacg gtgaaggttg
      421 catatatttt tgtttcatat attgtttatg gtatttttct gtcaacaatc aatatccctt
      481 acggagcaat ggcagcagcc attagttcaa atcctaatga ccgcacctcg ttatccacat
      541 tccgaagcat tggttctgcg attggcgggt caactacagg gttctttatc ccgattttga
      601 tgtacaccca taacaagagt gggcagcaag tcgtttcggg gaatcatttc ttttggattt
      661 ccatcggatg tgcggcaatt gcatttgtcg catacatgct gacttgtaaa ttgactactg
      721 aacgagtaag agttgtcaaa aacgaaaaag tgtctgcttc tcagttggta aaaggtttgt
      781 tagctaaccg agcactgatt attttagtca ttgttgacat tttcattgtg atcaatcaga
      841 ttttagctgg gacgaatatg acttatttat ttaatgatta cttccataat aaacaagcaa
      901 tgtctgtcgc tcttttattt acttacggaa cagtagtagt tttagcacca tttgctacaa
      961 agctgacaaa attatttggt aaaaaggaag caagcgtatt tgcgttactc gcttcttcat
     1021 gtatgtactt gctgatgtat ttccttcata taacaaatgc atgggtatat ctgattcttt
     1081 tatttattgc tactttagga tctggtttat tcaatttgat ggtctgggca ttcattactg
     1141 atgtgattga ctatcatcaa tataagacag gcttaagaga agatggaaca gtatacggag
     1201 tgaattcttt tgcgagaaaa gtaggacaag catgcgcagg agcagtcgga ggatttatgc
     1261 tagcactcat tggttaccaa tcgtctacaa caggtggagc catccaatca gttttagtac
     1321 aagaaagaat ttatacgatg gcaaacttgc taccagcatt ctgtttattt gtatccgcaa
     1381 tcattttatt agtagggtat ccgctgaatc gttcgaaaac tgtgtttatt ggtgaagaat
     1441 taaaaaaaat caatgaaaga taaggagaaa agtattgcag acaaaaaaag agttcatcaa
     1501 tgctttagac tttaaaacaa acgacattca acaagatgat acagtaatgc tgcaaaaggc
     1561 aatcaatcaa ggtgcaaaag aacactcgcc cgtatttatt ccaaaaggta tttatttagt
     1621 cggcgcactt tttttaaaag ataagagcca tctcattttc gaagaagggg ctgttctcaa
     1681 gggaaaaaca gagatcgaag catttccaga gattgataca cgagttgctg gagtagaaat
     1741 gaaatggcca gcagcgattt tgaatgttct ctcagcaaaa gatattctta tagaaggaaa
     1801 aggaataatc gacggtcaag gagagcattg gtgggaacta tattggggga aagaccaaaa
     1861 aagcggaaca agagctgaat atgatcgaaa gggactgcgt tggattgcag attatgctat
     1921 taagcgtccg cgggcgtgtt tactgtatca tgcagaacat gttgtcattc gtgatttgat
     1981 ttttcaaaaa tcaggttttt ggaacctgca aatcacttat tctaacgatg tattagtaga
     2041 aaaaataatc atccgtcata atgatgggcc aagtacagat gggattgata ttgattcttc
     2101 aacagatgtg cgtatctatg aatgtgattt ggcctgtggt gatgattgta tcgctattaa
     2161 atctggcagg gacggtaacg gtgcaagagt caatcgaaaa tcatcaagaa tcgagattgc
     2221 gagatgtaac attcgttcag gttacggtgt aactattggt agcgaagtta gtgctggagt
     2281 tagtgatgtg tatattcacg atatcgattt ttttcagtca gactgtggta ttcgaatgaa
     2341 atcttcaaga gaaagagggg gagtgattga aaatatcagg gtagaaaacc tgaatatgat
     2401 ggatgtacaa tttccgtttt cttggataat ggactggcat aatgaatata atagaaaaaa
     2461 tagcaatgat cttgaacgaa tgccagaaag ctggcaagca gtggctgaag aaatacctga
     2521 atcaaaacaa atgacaaaag tcagaaacat acatgtcaaa aatgtacagg caagtctttc
     2581 tccaggctat ccacttcctt cccgggcttt tgacttggta gcatttcctg aaaaaactat
     2641 agaagatgtc tgctttactc attgtacaat aactgcaaaa gaatttggac gaatcgaagc
     2701 agttcaaaac ttatgtttcg aaagctgtat tctatcaata gaaacaggga acacagttgc
     2761 aaacaatact tttgacaatc gttgaaatta
//
LOCUS       DADJYG010000224         2787 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.232, whole genome shotgun sequence.
ACCESSION   DADJYG010000224 DADJYG010000000
VERSION     DADJYG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.232"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..443)
                     /locus_tag="JZ382_002648"
     CDS             complement(<1..443)
                     /locus_tag="JZ382_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2428783.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="JZ382_002649"
     CDS             513..863
                     /locus_tag="JZ382_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY2428784.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="JZ382_002650"
     CDS             1277..2095
                     /locus_tag="JZ382_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAY2428785.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="JZ382_002651"
     CDS             complement(2168..2527)
                     /locus_tag="JZ382_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAY2428786.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          890 a          427 c          519 g          951 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc tacaatattt
     2761 aaaacgcttc cttagtctat tatatat
//
LOCUS       DADJYG010000225         2780 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.233, whole genome shotgun sequence.
ACCESSION   DADJYG010000225 DADJYG010000000
VERSION     DADJYG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.233"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..197
                     /locus_tag="JZ382_002652"
     CDS             <1..197
                     /locus_tag="JZ382_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428787.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="JZ382_002653"
     CDS             314..811
                     /locus_tag="JZ382_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAY2428788.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="JZ382_002654"
     CDS             786..1292
                     /locus_tag="JZ382_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2428789.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..>2780
                     /locus_tag="JZ382_002655"
     CDS             1276..>2780
                     /locus_tag="JZ382_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAY2428790.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          515 c          600 g          826 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc
//
LOCUS       DADJYG010000226         2764 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.234, whole genome shotgun sequence.
ACCESSION   DADJYG010000226 DADJYG010000000
VERSION     DADJYG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.234"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(122..499)
                     /locus_tag="JZ382_002656"
     CDS             complement(122..499)
                     /locus_tag="JZ382_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428791.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWVFVLPLLLISLFIMFKMVRK
                     KERFLIFIWYAASVAVASLMIELALGSWEACIFALFFAVLYLIIYIPYRKRKSRIGKK
                     GRHAATFSSTDSPEQSRRARRRR"
     gene            complement(800..1681)
                     /gene="galU"
                     /locus_tag="JZ382_002657"
     CDS             complement(800..1681)
                     /gene="galU"
                     /locus_tag="JZ382_002657"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY2428792.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LNSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTHVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQSSAVAVMRV
                     PENETSKYGIIDPKTDNEKGRCRVNGFVEKPELGKAPSNLAIIGRYLLTPKIFEILAT
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLHHPEIGA
                     SLSAYIIQLGKELRTEN"
     gene            complement(1724..2743)
                     /locus_tag="JZ382_002658"
     CDS             complement(1724..2743)
                     /locus_tag="JZ382_002658"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY2428793.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINTY
                     HTNQHFLPDLTIPESIIGYKELAEAVDDADAILFVVPTKAIRSVAQELIGKMNTKPVI
                     IHASKGLEQDSHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQELFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVMYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          757 a          622 c          437 g          948 t
ORIGIN      
        1 attccttttt gaaaagattc ttttaaataa gtaaaaaaag tttttttcac caattaaata
       61 aatctctcaa ctcaataatt tatccattct tttatctttt tttgcgtata catactaaat
      121 attaccgtct tcttctcgct cttctgcttt gttctggaga atctgtacta gaaaaagtag
      181 cggcatgtct accttttttt ccgatccgag actttctttt cctataagga atataaatga
      241 tcagatacaa tacagcaaaa aataaggcaa atatgcaagc ttcccaacta cctaacgcca
      301 gttcaatcat caagctagca actgcaacag acgctgcata ccagataaaa atcaggaatc
      361 tttctttttt tctgaccatt ttgaacatga taaatagact aatgagcaat agcggtaaca
      421 caaataccca gttacttcct tccatcagcc acttgagtat atcaagtagc gactgcgcaa
      481 cagcgactaa ttttcccata gtggacacac acctttttta taattaaaaa aattcgcctt
      541 tttattctac tatgaatctt ctggattggc tttcatttct cttatgtaat cattttcttt
      601 tatgtaactt aaaaaaaaat taaaccatta gcagaaaatt tcctaaatat attcacgaaa
      661 gtcttttttt atctttcatt tttcctcgtc ttcattcact taacgtcaaa aaataaaaac
      721 gacagttgcg caaatgacgc acaagctgcc gctttaacac ccatttgatt tttattctca
      781 gcaggtcact tatttccagt taattttctg ttcttagctc tttgcccaac tggataatat
      841 atgcgcttaa cgatgcaccg atttccggat gatgaagtcc atattcaata tttgtcttca
      901 tatatcctag cttatcgcct acatcatatc tttttccttt aaattctctc gcatagaccg
      961 cttctgtttg gttaagggtc tggagcgcat ccgttagttg tatctcgttc cctgctcctg
     1021 gttcttgagt tgctaatatc tcaaatattt tgggagtcag cagataacgt ccgataatgg
     1081 ctaaattgct tggtgctttt ccaagttcag gtttttctac aaaaccattt actctgcaac
     1141 gtcccttttc gttatccgtt ttaggatcaa taattccata tttagaggtt tcattttctg
     1201 gtacacgcat gacggcaaca gcagaagatt gtgtttcttc ataatccatc atcaattgtt
     1261 gagtcagtgg gatatcatct ttcatcaaat catccccgag catgacaaca aatggttcat
     1321 tccctatgaa tgcttttgct tgaagaacgg catcccccag tcctcttgga tgagattgac
     1381 ggatgaagtg aagattgaca tgagtcgttt cttcaactaa agcgagtagt tctgtttttt
     1441 ccttttctcg caaattgttt tccaattcga tatttgcatc aaaatgatct tcgatgggtc
     1501 tcttttcttt tcccgttacg atcaagatat cttcaattcc cgaattcaat gcttcttcca
     1561 caataaattg gatcgttggc ttatccacga ttggaagcat ttcttttgcc atggcttttg
     1621 ttgccggcaa aaaacgtgta cctagcccag cggcaggaat aactgctttt ttgaccttca
     1681 cttgctgttc ctcctaaacg acgattacct gttaatcatc tatttaacga agagaaaatt
     1741 cattctccat ttttccgtca cgtagcatga tttcttttgc ggctttttta atatcttctc
     1801 cattatacat aacattatag atggtttgag taattggcat ctcaacacct agctgttgtg
     1861 ctagctcgac tgctgctttt gtcgttgcca caccttcaac gaccattccc atgttttcca
     1921 aaacttcttc cagtttatgc ccttgtccta gtaagttacc ggcacgccaa ttacgggaat
     1981 gaacacttgt acaagtaaca atcaaatccc caacgccgct caaaccgata aacgtcagcg
     2041 gattggcacc cattgctaca ccaagacgac taatttctgc taatccgcga gtcatgattg
     2101 ccgcttttgc atcgtctccg aaccctagcc catggatagc accggcacca atagcaatga
     2161 tattttttaa tgctgcgcca gtttctacac caatcacatc attatttgta taaatcctga
     2221 aataatcatt catgaataat tcttgtacat aagcagctgc tttttgatca atacttgcag
     2281 ctgtaatcgt tgtgatatca tgaactgcta cttcttccgc atgactaggg ccagataata
     2341 cgacgattgc ctgtcgtttt tcttctggaa tttcttcagc caaaacttct gagattcgtt
     2401 tatgcgaatc ttgttctagg cctttacttg catggataat gacgggctta gtattcattt
     2461 taccaataag ttcctgagcg actgaacgga tcgcttttgt cggaacgaca aacaaaatag
     2521 catctgcgtc atccactgct tctgccaatt ctttatatcc gataatcgat tctggaatcg
     2581 tcaaatcagg taaaaaatgt tggtttgtat gatacgtatt gatttcgtca atctgttcag
     2641 gtacatttcc ccaaatacgg acatcgtgcc cgttttctgc caatgtctgt gctaaagcag
     2701 tgccccatga tccaggacca agaactgcga ttttttgttt catggatatt cctccaaata
     2761 aata
//
LOCUS       DADJYG010000227         2755 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.235, whole genome shotgun sequence.
ACCESSION   DADJYG010000227 DADJYG010000000
VERSION     DADJYG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.235"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(149..1201)
                     /locus_tag="JZ382_002659"
     CDS             complement(149..1201)
                     /locus_tag="JZ382_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2428794.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1214..2152)
                     /locus_tag="JZ382_002660"
     CDS             complement(1214..2152)
                     /locus_tag="JZ382_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY2428795.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          897 a          588 c          412 g          858 t
ORIGIN      
        1 ttcttctaca ttttaaaaaa tcgaatggca ctgaagagtt cagttgtcag catcaatctc
       61 ctactcaaaa aggcaaaaaa acgtaagtca gatgttgttg acatctcact tacgcatcca
      121 ttgattgaaa atttcttagt aaaatctact agcctaaaaa tttgaagact tcttctcgat
      181 catacagttt aaatactttc tcgcataaaa attgagtaat tagtgccgca aaaacaggga
      241 tgactaacca acaaagaaca acaagaaagc catttagccc tgcatctaaa gaagccaacg
      301 gaccaacaag tccgaccacg ccgaaaccag cagaagcagg cgtacctgaa atagaaaata
      361 gagcgacagg aatagctgta atcactgcac taactaaaca aggaataaga atcacaggtt
      421 ttctaaatag attaggcatc atcatcttca tagcgcctaa agcaatcgca attgtcacac
      481 ctgacttatt aaccttccaa gagttcacta ctaaaacaaa agtggttgca gctacgccca
      541 tcgcagccgc accagcagaa acaccatcta gttggattgc cattccgatt gctactgttg
      601 aaataggaga gataatcaaa aaggcaaagg aacaaccaat caagatagac attaacagcg
      661 gttgtaaggt agtgaagtta ttaatcaatg atccaattgc agaagtaatt gctgttacaa
      721 aagggaataa ataaaaacca agtaaacctg cacctacacc tacaacaatc ggtgttaaca
      781 caatcgacac tgacccaaaa ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg
      841 ctgtcagcat cgtattaata atatcgcctg taccagcacc aacgtaaaca cccgtttcac
      901 ccaaatcagg tgtgaatttg actacaccag aaccaacaaa agaagctccg gctacaacca
      961 tcattctcat cggatcaaat ccaaattgta aggcaatcaa accaccaatg atcaaaggtg
     1021 ttgctagttg aaaaataact gctaactgtg cgatcgttac cgcaaaggga tacgcactaa
     1081 aatattttaa aatggcacct aatactgcat taggaatcaa tccaataatg attcctgttg
     1141 ctgtaccagc gaggacttta ttcagaaaaa ttttcggtga tattttttgt gaagtttcca
     1201 ttcaatctct cctttatttt acgttcaaac tatcttcttt tgcatggaca agctcagtca
     1261 agaaatcaca ataaggcgtc gcaacaccat atttcttccc ttttctagaa attgcgccat
     1321 tgatataatc gatttctgtt aatcgatggt tcttaatcaa gtcttgatac attgaaggat
     1381 aatgtaaccc aattgtttcc gggtcaaaac aagattcaca atgtgcaatg acttctggga
     1441 catctagttc aatcttctct actgctgcta ctttggcaaa ctcattgaca atcgtcgcca
     1501 ccattttatg agcagtcgat gtttttccta gctcagccat attaacgtct aagattgtgc
     1561 atagtccgtt cattgttccg ttcacacatg ctttgcgata aatagaatag tgaatattgt
     1621 cagaaaaatg agcatttaat cctgattcag acaatttgtc tgctaatttt tttgcagctg
     1681 cttcttttcc atcacctaaa ttttgtaatt ctactgaacc acttccaaat aatttgacct
     1741 gaccaggacc ttcaagacct gccgtccaca tggtatttcc aatatagata ttttccattg
     1801 gtacaaattt ctcaataata tcttcatgcc cgataccatt taatagacat aagacctctg
     1861 tatctttttt gattaatgat tggatatcct gcagcatttt ttccagctgc atcgctttgg
     1921 taaatagaat aatcagatca acttgatctt ctttttctac ttcggattga agaacgattg
     1981 gtagttttgc ttctacctct ttaccattaa aatttgcttg taatccatgt tctttgattt
     2041 gttggacatg ttctgcccag ccatcgatta ataatacttc gttcccactt tggtgaagca
     2101 tcaacccaaa gcgacttccc attgcgccag cgcctgcaat tgctattttc attgattctt
     2161 cctccttttt ctttctatta tactcatttg agaatctttt cacaacctcg ttttgttatt
     2221 cttctcttat ttatcatgtt tatatccacc attcaagtta ttttatcagc aaatcaataa
     2281 aaactggaat agaatcccaa tttcatcgta agagaaacta gactagcaat aagaaaatag
     2341 aaacattcat agaaaaaatc tcactcctct gattataaaa gagggggcat taacctcgtg
     2401 tatactaaaa ccttccattc attcgatgga tgattttctt tttgtgaaaa aatattcatt
     2461 gtagtacgat gttgcgcaac acagacggat ggtattgctt attatttatt aaaatcgaac
     2521 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
     2581 tattatcatg aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt
     2641 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
     2701 ccggaattat ccaagcccag tctaaataat tttttaaaat ctcatcatat tttgc
//
LOCUS       DADJYG010000228         2712 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.236, whole genome shotgun sequence.
ACCESSION   DADJYG010000228 DADJYG010000000
VERSION     DADJYG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.236"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..375
                     /locus_tag="JZ382_002661"
     CDS             <1..375
                     /locus_tag="JZ382_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428796.1"
                     /translation="EWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANTP
                     EEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYIV
                     KAKVSKRFIFNPKLEIYSVVIE"
     gene            700..1317
                     /locus_tag="JZ382_002662"
     CDS             700..1317
                     /locus_tag="JZ382_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428797.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1358..1657
                     /locus_tag="JZ382_002663"
     CDS             1358..1657
                     /locus_tag="JZ382_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428798.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1691..1858
                     /locus_tag="JZ382_002664"
     CDS             1691..1858
                     /locus_tag="JZ382_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY2428799.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1903..2508)
                     /locus_tag="JZ382_002665"
     CDS             complement(1903..2508)
                     /locus_tag="JZ382_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428800.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          949 a          409 c          495 g          859 t
ORIGIN      
        1 gaatggaata atctgactca aaataataaa aataattctg gatgtggaat tatctttatg
       61 tttgttatag ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt
      121 gcgaatactc ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct
      181 tatgatccaa gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg
      241 tggtttgaag ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat
      301 attgtaaagg caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt
      361 gttgtaatag aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg
      421 tgaaaaataa aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa
      481 tggcagaccg ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg
      541 ggagtgtaaa cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt
      601 tatgtatggc ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt
      661 tttgaaacaa ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc
      721 cgggtcagta gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga
      781 atccctgaag aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa
      841 tatcaattaa tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat
      901 agactaggca gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag
      961 cagattgata tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta
     1021 gatgggttag agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa
     1081 gacgagcgga aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag
     1141 ggtgtaaaat tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct
     1201 gactggaaag caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga
     1261 aatacctttt atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag
     1321 atataacagc tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt
     1381 cgtttttact ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc
     1441 tttaaacggc cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca
     1501 gtattctaag tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga
     1561 cttgattgcg ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat
     1621 tagtcaacag ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag
     1681 gaaaattttt atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa
     1741 tcgaaatatt gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca
     1801 aaaaaattac ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa
     1861 acgaaatagt gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt
     1921 ctttatttta taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg
     1981 aatgccttgc tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt
     2041 tgagttaata gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa
     2101 tactccttgc cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag
     2161 cagtgataaa aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc
     2221 taaaaactgt gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt
     2281 attttttata tcttcataat ctcttcctaa acgatctagt gatgtaacga tgacgcaatc
     2341 accttcacgg atataattta gtaatttttg aaactcagga cgctcagtat ctttaccact
     2401 ggctttttct tggaataatt tatcaatgtt aagtccttcc atcattgcca attgtcgatc
     2461 caaattttga tcttgtgaag atactcgaat atagccaatt tttcccataa aaaactcctt
     2521 tttaaaagtg atataattta catgtaaaat ttacttgtag ttttactgct atcatagcat
     2581 ttttaaaaat aaattttatg tagacttaaa atttacttca tggagggttt aaatggatga
     2641 aatcaaaagt aacaaaaaag catttggcat ttatttagga gaacctagta cagaacaatt
     2701 aaaccaattt tt
//
LOCUS       DADJYG010000229         2652 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.237, whole genome shotgun sequence.
ACCESSION   DADJYG010000229 DADJYG010000000
VERSION     DADJYG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.237"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            15..1022
                     /locus_tag="JZ382_002666"
     CDS             15..1022
                     /locus_tag="JZ382_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAY2428801.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1034..2038
                     /gene="neuB"
                     /locus_tag="JZ382_002667"
     CDS             1034..2038
                     /gene="neuB"
                     /locus_tag="JZ382_002667"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAY2428802.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2035..>2652
                     /gene="neuC"
                     /locus_tag="JZ382_002668"
     CDS             2035..>2652
                     /gene="neuC"
                     /locus_tag="JZ382_002668"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAY2428803.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQ"
BASE COUNT          980 a          338 c          526 g          808 t
ORIGIN      
        1 aggaggagat ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca
       61 ttggaagtga aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata
      121 gtagaggtga agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga
      181 gatttttaat tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg
      241 atattatttt tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg
      301 aagctgtaaa aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata
      361 atgtaaaaca ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg
      421 gtgcgacaaa gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa
      481 cagaaacaat attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta
      541 ttcctctttt tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata
      601 tgacacgatt tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga
      661 attcaaaatt tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag
      721 ctcaatcatt aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa
      781 aagttgtagg tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa
      841 ttgaacgctc atatgaaaat gataaaatgt atatcattcc accacactat catgctactg
      901 acgaactgta caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag
      961 aattaatgag tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt
     1021 aaggagtttt atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa
     1081 tggaagtcta gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc
     1141 ggttaaattt caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa
     1201 atatcagaag gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga
     1261 attatctcaa tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt
     1321 tttgtctact ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg
     1381 atttaaaatt ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta
     1441 tgggttgcca gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt
     1501 agattgtttc atagatagtc cagttactat tttacattgt acttcaaatt atccggctag
     1561 ttctgaagat attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt
     1621 tgggtactcc gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc
     1681 taaggtaatt gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc
     1741 ttctttagat cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt
     1801 tttaggtgat ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag
     1861 aaaaagccta gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct
     1921 tacaataaaa cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa
     1981 aaaagttaaa agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa
     2041 aaaatactta tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag
     2101 aatttactac aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat
     2161 aaatatggat ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt
     2221 caaagtttgt ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata
     2281 aatggtttca caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat
     2341 agaggagaaa tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat
     2401 attcacggag gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa
     2461 atagcatctg ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg
     2521 gaagattctt ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat
     2581 agattaccta gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa
     2641 tacgttttgc aa
//
LOCUS       DADJYG010000230         2646 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.238, whole genome shotgun sequence.
ACCESSION   DADJYG010000230 DADJYG010000000
VERSION     DADJYG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.238"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="JZ382_002669"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="JZ382_002669"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY2428804.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          953 a          461 c          585 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
     2641 aaaaag
//
LOCUS       DADJYG010000231         2646 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.239, whole genome shotgun sequence.
ACCESSION   DADJYG010000231 DADJYG010000000
VERSION     DADJYG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.239"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            441..647
                     /locus_tag="JZ382_002670"
     CDS             441..647
                     /locus_tag="JZ382_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAY2428805.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            647..898
                     /locus_tag="JZ382_002671"
     CDS             647..898
                     /locus_tag="JZ382_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428806.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            913..1350
                     /locus_tag="JZ382_002672"
     CDS             913..1350
                     /locus_tag="JZ382_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428807.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1343..2050
                     /locus_tag="JZ382_002673"
     CDS             1343..2050
                     /locus_tag="JZ382_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428808.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2431..2610
                     /locus_tag="JZ382_002674"
     CDS             2431..2610
                     /locus_tag="JZ382_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428809.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          959 a          447 c          552 g          688 t
ORIGIN      
        1 ataaaagaag agctgcagtc ctaaatgagg attgcagctc tttttgtttt acatagaaac
       61 aggaaaagac ctttttagga agacacaagt gccttctcaa ttgaaaaacc tttctctcac
      121 tccaaccgcc accgccgttc gagaagaaag gaagtgaacg ttgcacgttc actaagatat
      181 ggtgtcttgc aaagcaagac aaaatagaaa aaacttcctc tcaccaaaag gttcgttcct
      241 gttcgctatc tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa
      301 gcgacaagga caagggtaaa tggcacgctg cgcgcccttg tccttgtcgc ttttcgttga
      361 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaaataa aacctttcct
      421 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
      481 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
      541 aatgtatcaa aagctatcgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
      601 cttatggcga ttatgttttg attgatgaga aagtaaagaa acggtgatga aaacacaaca
      661 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
      721 aaaagtagca gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
      781 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
      841 tcaagaaaac atggaaactt atgaaatcga atattggtta accaaattag agaactaaaa
      901 aggagaaaaa atatggctca aaattactat gacgaatttg tcaaattacc tttggataaa
      961 atggcacaaa aaatggagga tatgacgttc ctataccatg aaacacgtgt acccaaaaaa
     1021 cattacaagg aaaaattgtc agtcgcagtg gaagaaatga ttgaatcagg tgtcgaaatg
     1081 aatctcattg cgacgtacta tcgaacactg gaagaactga aaaaacaaaa tggaaaatgg
     1141 tttttccaag ccttactatg cttggaagta ggtgtcaaac caagcactat caaaccttcc
     1201 gaatatcaag ccttagaact gacttatgca aaattcgcac agattaagaa agctaaagca
     1261 atggctagcg actggctaga tacttttgaa aatatcgaaa aaaatggtgc attttatacg
     1321 attgaatcgg aggagaaaga taatgaataa ggtggtttat cagctaacaa cagaagatgt
     1381 ttcgcaattt ttaacaaaca aagaacactt gaatgaagta atcagacgga ttcaaaaaga
     1441 ctttagttgt atggaagaaa tgaagtactt tattcaaaga gtggaagaac agttgtatat
     1501 ggaaagtgaa acgagtcaaa cgaaggttac agtcagccat aatcgtttag acgtgtataa
     1561 acgtcgtcct gacacattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata
     1621 tatcatgaat gtttacctag aaaggagtga gctggtcgaa aactttgtca gctacacgat
     1681 ttgcaggaaa cactattact cagaatcaga atattattca atttttgaga gcatggaatt
     1741 aatggaagaa gaaatggaag agctaaacag caaagatgcg acggtagacg attattacct
     1801 tgtatttact agatttttga cgtgtgggac ggaatctatc gtgttaatgg gaatggatta
     1861 tgaactcact gtacccgaag catttttgag ggaatatgta gaagaagtag ataacccagg
     1921 cgacgactgg tacacgactt taactgattt tttagacacc tacacttacg acgatacaga
     1981 agaaatccaa aatgaacttg accgtaaacg aatgccatat caacgatttg aaaggaaaaa
     2041 aggtcaataa ccatttatac cgaaaagtga ggatgtgtga aataagaaca aaaaaccttg
     2101 aaattaaggg aatggttctt atttcgcacc aatcgatcca accgcccccg ccgcttaggt
     2161 ggtgcgaaat aagcgggcaa agcccgataa cctaagagaa agaaggagat ccatatgaat
     2221 aaaatcattc ctctattgtt cgtactgttt gttggcgaga tacttatgcg tcatggtgca
     2281 gctctacatt taaacttcgt agtattctta ggaatactgg tattttgttt gatcttttgg
     2341 cgctttgtgt taacccttgt gagtctaata ttcaagttga ttgcagccat atttttaata
     2401 gggttatttc gatgaaagga gagagtacta atgccaattg caataggaaa taaacggtta
     2461 cccgtgacgt tggatgaaaa gagacaaaaa gaattgcagc aactaaagca gaagtacggc
     2521 aaaagtgaat ccaggattat gtgtattgcg ttagatttat tgattgccca agaaaaagca
     2581 ggatttgagg taccagcact caaaaagtga cgtcactttt tatcctaaaa actaaaagtg
     2641 atagca
//
LOCUS       DADJYG010000232         2639 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.240, whole genome shotgun sequence.
ACCESSION   DADJYG010000232 DADJYG010000000
VERSION     DADJYG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.240"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1237..1902)
                     /locus_tag="JZ382_002675"
     CDS             complement(1237..1902)
                     /locus_tag="JZ382_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428810.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..>2639
                     /locus_tag="JZ382_002676"
     CDS             2028..>2639
                     /locus_tag="JZ382_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAY2428811.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIG"
BASE COUNT          852 a          417 c          473 g          897 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattgga
//
LOCUS       DADJYG010000233         2632 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.241, whole genome shotgun sequence.
ACCESSION   DADJYG010000233 DADJYG010000000
VERSION     DADJYG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.241"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..1149
                     /gene="xerS"
                     /locus_tag="JZ382_002677"
     CDS             64..1149
                     /gene="xerS"
                     /locus_tag="JZ382_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAY2428812.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1433..2217)
                     /locus_tag="JZ382_002678"
                     /pseudo
     CDS             complement(1433..2217)
                     /locus_tag="JZ382_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2252..2401)
                     /locus_tag="JZ382_002679"
     CDS             complement(2252..2401)
                     /locus_tag="JZ382_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY2428813.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(2394..2603)
                     /locus_tag="JZ382_002680"
     CDS             complement(2394..2603)
                     /locus_tag="JZ382_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAY2428814.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          898 a          467 c          372 g          895 t
ORIGIN      
        1 ataattggta tagtccaatt atagagatac tcttctcccc tacttattga aaggatgaat
       61 gacatgaaaa atacagaaat atatcaaaaa atagatacaa tcatacttca tatgcccgat
      121 tatataaaag attttgttca agatcgtgag gataaagatc aatcacctcg aaccacttta
      181 gaatatctaa aaaattataa gctcttttat gaatggcttc tgagtgagtc tatcgttcct
      241 gatacttcta tcacctcgat aagacagtta actgcctatg acctaaactt gtacaaatct
      301 catttgaaac gtcgcacaaa agaaaatgta aaaaaagaga caacaaaaac taagctcaaa
      361 gataataata acttaggtct atctacttct actatcaata gaaatatcac ggcattaaag
      421 gttctattta aatatctttc gaaaagttcc aataatcctc taggaaaacc atacttagac
      481 aataatccta tggatcaagt agcaaccatc acagataaaa ctacgttagc tgcacgtgcc
      541 aatgccattg agaaaaaatt atttctagat aaggatacac aaaattatct agattatatt
      601 gccaatgaat ataaaaatac tttgagtaaa cgagctttga tctattatca acgagatgta
      661 gaaagagacc tggcgatcaa tgcactgatt ttaggatctg gattgagact ctccgaggtt
      721 ataaatatta atttagatga cctatcttta gataagaata atgtggtagt aactagaaaa
      781 ggaaataaaa gagatgctgt caatatagca gcattcgcaa tggaatattt agctaattat
      841 ctagctattc ggaaagaacg atataaagta acagaaaacg aaaaagcttt atttctagca
      901 atttaccaag gagaagccaa gcgtatatcc ggtattgcaa tcgaacggat ggtagcaaaa
      961 tattcaaaag gctttagaat tcaagtcagc cctcacaaac tgcgtcatac attagctact
     1021 agactctacc aacagacaaa ttcattggta ttaactgccc aacaattggg acattcaagt
     1081 accaatacaa caactttata tacccatata gacaatgcgg ctacgatcga tgcattaaat
     1141 gctttatgag tataatgtat aaataggcgg aaataagaaa caacacgcta gaaaagcctg
     1201 tattatagtt tttctagtgt gtttcttttg gtgtgatcct acttttgttt atcatttatt
     1261 ttttattatc gtaaataggt gtcggtttga ttttagaaaa attagtttgg atataatctg
     1321 tgggatcatt tgttatctct ctcaatgaat ttagatacaa atgaccataa gttcctaaag
     1381 tcgtttgtac atcagcatgt tttaaccttt ctttaattcg aagtgcatct ttttattgtg
     1441 atataagcaa agaagcatga ctatgcctta atccgtgtat gcggattata tgaacaccaa
     1501 cgatcgttgc atgtcttctc ataatatgac ggattgtata tttctgagta ggtgttccat
     1561 tataagaaag tacaaacgac atgcctccta ttgaaacttg acgctccttc caagctttta
     1621 aaaaggtaat tgtattgtcg tctagttcga ttgtacgaat actagaagcc gtttcgggtt
     1681 ctacaaactc ataggttttg gctctcttat aatagagtgt tttattcaca cgcaagtttt
     1741 tattttcaaa atcaatatct ttccaagtta atgcagtggc ttctcccaaa cgcataacgg
     1801 tcataaaaag taaccacata caaataaaac caaaatgttg gtacaaatca tcaatataaa
     1861 aagttgaaat aacttttgta aattcttctt ttgtccaaaa atcaactgta gttttccttt
     1921 tcttcacatt tccaataact tttgcaggat ttttttcaag tagtcccaaa acaactgctc
     1981 gatctaatga catctgaaaa atcccataca tgtttcttac ataagaattt ttatatttgc
     2041 tcaataaatt attttgccac ttttgaatgt caattggacg tatttctctt aactccattt
     2101 taaagaaata agaaaagttt ttttgattgt agataaccga tttttatatg ttctttcttc
     2161 tactttccct tgataataag gtaggaataa tttataaaaa aactcctcaa acaacatagt
     2221 actttgagaa gtttctgctt cttttccata attagaaaca agttcggtat acgctttttt
     2281 tcttctttaa ctgttttaac ccctctacgt ttttttgtac tcttttccct gttcgtttgt
     2341 cgaatcccaa actagcagta aaatgatatt tcccattttt atctttttta aaattaacca
     2401 tgattctgaa aatcctcctc tacctctatc ttcataccta aaacttcttc tacagattca
     2461 acaggtacta gtcctaatct tctattttcg taataggtat agctttttga aaccataatt
     2521 gcttttgcct gtcgtatgat tctctcagac tgatacttcc cataacctaa tttcattaat
     2581 tcattttttg ttataaccac catttaacca ccacattttt tattcaaata ta
//
LOCUS       DADJYG010000234         2585 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.242, whole genome shotgun sequence.
ACCESSION   DADJYG010000234 DADJYG010000000
VERSION     DADJYG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.242"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1069..2037
                     /locus_tag="JZ382_002681"
     CDS             1069..2037
                     /locus_tag="JZ382_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428815.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            2054..2578
                     /locus_tag="JZ382_002682"
     CDS             2054..2578
                     /locus_tag="JZ382_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAY2428816.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT          973 a          439 c          463 g          710 t
ORIGIN      
        1 ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa aagcggttat
       61 ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac actttagtga
      121 gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt ccgtaggacg
      181 tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca aaaaaagtta
      241 aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg aatccgccat
      301 gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac ttttaaaaaa
      361 gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga cgtttgaaac
      421 gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa ttttagaggg
      481 cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg cgctttaaga
      541 acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag gctcatagct
      601 tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact gcgcagtata
      661 atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga ttcccagttg
      721 agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg ggaaattttg
      781 tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc gtcggctata
      841 tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt ttatctacat
      901 cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttgt
      961 acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg cattttgtat
     1021 ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat gtctaatgaa
     1081 atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac accagtcgaa
     1141 atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa aaaagtacaa
     1201 tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa tgttcgattc
     1261 atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg tagaagaagt
     1321 gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat cgacggaaaa
     1381 tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt acttaataat
     1441 cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag cagttattca
     1501 aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta tttttcaaaa
     1561 gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac gattgatcaa
     1621 cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt aacaatcaaa
     1681 aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac atttaaacct
     1741 gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga acaaaacaaa
     1801 gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc attacaagaa
     1861 aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa gttagaaaga
     1921 gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa ttccgatatt
     1981 ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa agattgaggg
     2041 gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat taggagttag
     2101 taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt tttcgaaaat
     2161 cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa tgttttttaa
     2221 cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg aaaaccacaa
     2281 aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac agttaaatca
     2341 aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa aattactcga
     2401 ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc aggaacaact
     2461 acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt ctgaagaaac
     2521 agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct ggcaataaaa
     2581 ccagc
//
LOCUS       DADJYG010000235         2557 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.243, whole genome shotgun sequence.
ACCESSION   DADJYG010000235 DADJYG010000000
VERSION     DADJYG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.243"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..270
                     /locus_tag="JZ382_002683"
     CDS             <1..270
                     /locus_tag="JZ382_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAY2428817.1"
                     /translation="ELVKLEMAETGAGVRSSRHWKAAANIEFYYNEIQKGFDQMRELD
                     RQTNWSKKLHQDRFKFVEKYKEILEEYLRRTANDKKTRSIQHGFI"
     gene            230..475
                     /locus_tag="JZ382_002684"
     CDS             230..475
                     /locus_tag="JZ382_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428818.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            472..891
                     /locus_tag="JZ382_002685"
     CDS             472..891
                     /locus_tag="JZ382_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAY2428819.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            888..1445
                     /locus_tag="JZ382_002686"
     CDS             888..1445
                     /locus_tag="JZ382_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAY2428820.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            1442..1738
                     /locus_tag="JZ382_002687"
     CDS             1442..1738
                     /locus_tag="JZ382_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428821.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            1815..2228
                     /locus_tag="JZ382_002688"
     CDS             1815..2228
                     /locus_tag="JZ382_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAY2428822.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT          904 a          378 c          593 g          682 t
ORIGIN      
        1 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
       61 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
      121 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
      181 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
      241 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
      301 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
      361 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
      421 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg
      481 aagtttagag cgtgggataa acgaaagaac gtaatgagag atgtagccgt cttgcatttt
      541 actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga attgaaatca
      601 tatcacgtgc gaaacatcga cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg
      661 gatatatttg aaggggatat agtattagtc agcgtgcgaa atggcttcga ttacttagat
      721 aataaagtct gtattgtcaa aaattcaata gattattccg gattagtttg tgccactgtt
      781 gatgaagact tagagtatca aatttttaac acagagctgt ttgaagaata cacgtacgaa
      841 gtcaccggaa atatatacga gaatagcgag ttattggagg aacagcgatg aataaacagg
      901 aagcaatcaa aaagttagaa agtattaaag cgataggaaa tgatgcaata gctgcttgct
      961 ataacgagag tataaattca ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac
     1021 cagttattcc acaactcgtg gccggttggc ttgagaaatc tacggaccct tttacaaaag
     1081 ctgaaaaaat agcgtattta atcaaatcta aagatggtga ttcatattat ttctgtgatt
     1141 ggtttgtacg agatggcata gtgacgcaag agcaaggaga agaattactc gcttgggcaa
     1201 caagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagagc
     1261 cgttatgggc aataaagaat gccgatggaa actatcttac taaatgtgct ttatggggaa
     1321 aagatggagt aaattatagt tttgaatgca atccatctca tcgattgctt ttcactgata
     1381 aagcaacagc ggatgctgca gcattgttgg tgaatggaac agtggaagag gtggtagaaa
     1441 gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt taatgctaga
     1501 tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt
     1561 ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa agatactgtc
     1621 aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca
     1681 caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga gcaaataaaa
     1741 aaagccggat cgctccgact gattcaataa atccaacaca tttattatat cacataaagg
     1801 agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg
     1861 cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag
     1921 atattaagtc gccgattatt acggatatgc cgaaggcacc aaagcacggc aataaggcag
     1981 aagacgcgat cattcagatg atggatatag aagcggagag agatgcgatt ctagcggctt
     2041 tgatggctct tagtctaatt agtcgccaga tactctacta cagcttctgt gtgccagata
     2101 gcttctcaaa ctacaggatt agccgtgaag tgggttattc agaaagaagt atacaacgga
     2161 tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaga ataattgctt
     2221 ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagctatt taccagtgat
     2281 attatggtag tgtcgaaaga ttagtgatag gtctaagaca aaataaaatg taagggagga
     2341 aatctccctc atcgtttaat taagcttcga taggcagcag caaataaact aaaggatgtg
     2401 ggggtcagct cctacagtta gttcatgtgt tgctgtctat taattgcaac tctttcggtt
     2461 ttattgagta tttactcaac ttaaaaaaac tgttgtttgt agatgtaagc tgttcctatt
     2521 tgtacatagt aaaaaatgat ataataaaaa tattacg
//
LOCUS       DADJYG010000236         2547 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.244, whole genome shotgun sequence.
ACCESSION   DADJYG010000236 DADJYG010000000
VERSION     DADJYG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.244"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(136..714)
                     /locus_tag="JZ382_002689"
     CDS             complement(136..714)
                     /locus_tag="JZ382_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2428823.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(734..1402)
                     /locus_tag="JZ382_002690"
     CDS             complement(734..1402)
                     /locus_tag="JZ382_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428824.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1417..2487)
                     /locus_tag="JZ382_002691"
     CDS             complement(1417..2487)
                     /locus_tag="JZ382_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2428825.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          740 a          555 c          387 g          865 t
ORIGIN      
        1 aatataccct gataatcatg agagaataac aaatttgtat ttatgaaaaa ctacctatat
       61 aaaaaaaaca agatataacg atcttcgtca tatcttgctt cttcacctaa ctatttttct
      121 ccctatataa cagctttacg cttctcctaa tacaccattc aaatataaat cgatcaattg
      181 cgccacattt agcggttctt ccacaggtct tttcgaccga gtgatgtaag gtgctaacac
      241 taagaaaaaa tgccttgctg cgatttcttt tgaaatccca tttcttagca cagattggtt
      301 tgctgaaaag atttcttcaa aaggttcacg ataatttttc ttccatagaa agaaaagatg
      361 atctcttgtt tcctttgaaa gattgctttc aagatcgtga agcatcagaa acaaatcgac
      421 cagatgtgtc tcaatcaaaa actgcgccat ttttgtcaag gttacttgaa atcccgtttc
      481 gcttgtttga acgatttctc tcaaagcttt tccaacatct tttaagtggt tttctaaaac
      541 tgccgtatat agtttttctt tatcaccgaa ataatgatag agattgggtt gggtgatccc
      601 aactttttgt gcgatctctc ttgttgaagt gccgagatat cccttggaca taaacaagtc
      661 agtcgcttct ttcaaaattt tttgctgtgt atctttgttg tatcgatgga ccatttttcc
      721 acctgctttt tggttattct ttttctttca atacaccatc ttccatcata aatattctat
      781 cgcatttgtc aatcagccgt gtatcgtgag taaccatgat cgttgcttta tttttgtctt
      841 ttgattcttt tgccaagata tcgactactt ctcgtgcctt ttctgaatcc aaactagcag
      901 ttggttcatc tgctagaata atggacggat cattgtacaa tgcacgagcg attgcgaccc
      961 tttgccgttc tccaccagat agttcttctg gatacttgtt gatcaactta tccacgccaa
     1021 gctgctcaaa taattctttt gcttcttttt gacgatgttc atgtttgatc ttatctacta
     1081 ataataactg cttttttact gtcagaaaag gaaccagatt ggaagcttgc aaaataaagc
     1141 caatttcttt aaaacgtagt ttaacgcgtt cactttcttt ttgattactg aattcttgat
     1201 cattgataaa tacagagcca ctgctaggcg tctgcaatcc gcctgccaaa gtcaaaaaag
     1261 tacttttacc tgaaccagaa ggaccgatga ttgcaacaaa ttccccttta tcgatactca
     1321 tggttgtttc tttcaatgct ttaactactt ggtcgccatc ttggaaactt ttttctactt
     1381 ctttaaattc tattgctttc atgctcttcc tccttcttat ccaattgcct tcaacggatc
     1441 gatttttacg attgtccgaa cagaaaatag tgcgcctaac acagctacta ccagcatcaa
     1501 caagctaatg acgccaaaaa acagccaatt gctttggaat ggaaccgcat caggtagaac
     1561 aagggatgtt cccactgttc ctaatagacc aagaagaata ccaacaaatg ataaaatgaa
     1621 tgtttgtgcc actacagaac gcgcaataaa tcctcctgtg atcccttgtg ctttcattac
     1681 accaaagata ttaatctttt gcatcgtcag tacataaatg aaaatcccga tcacaatagc
     1741 cgcaataacg atcaaaaatc cgatcataaa tccaaaagtc aatacttgcg cattatatcc
     1801 aggtaattca ttgatgaatt tagaaatttt gatctgttct aaatcttcag gtaaattact
     1861 gattttacct ctgacaacta ttgcgttaat ccgtgcattt tcacttgtat cttctttttc
     1921 gaagcggatc tcttggtaag cattgatggt tgtatagaga actggtgaga cattgaattt
     1981 ggcatgatcc gtaaagccag ttagtttaaa cgtcttgtca ctaccagata acttaactgt
     2041 atctcctacg gctagaccat attcttcttt caaactaata tcgcctactg cctcatcatc
     2101 gttatcaaaa gcttttcctt caacaagatt gggcatgatg aattgatttt tatcgatacc
     2161 gaagaagcta acattgattt ttccttcttc tgtactgtct ttactagtca cgactccagg
     2221 tgtctgggca agatacgcca ctttcccacc ttcgacttct tctgctatct tcttagtaat
     2281 catggacatc cctagatttg aatttgcatc tttcgaaagc acaatcgaat ctgcctccca
     2341 cttgtcgact gctgttcgat tatcttgagc taagccataa gctaacccag tcaaaaaaaa
     2401 taccaggtag gcgatcaaaa acatcacccc tgcaactaac gcataacgta actttgaatg
     2461 cataatttca tttaatgcta aaaacattta actcctcctc ctgaaataat cattttatac
     2521 aacaatttta tcacttgata aattttt
//
LOCUS       DADJYG010000237         2527 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.245, whole genome shotgun sequence.
ACCESSION   DADJYG010000237 DADJYG010000000
VERSION     DADJYG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.245"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            61..696
                     /locus_tag="JZ382_002692"
     CDS             61..696
                     /locus_tag="JZ382_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAY2428826.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            809..1444
                     /locus_tag="JZ382_002693"
     CDS             809..1444
                     /locus_tag="JZ382_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428827.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            1743..>2527
                     /locus_tag="JZ382_002694"
     CDS             1743..>2527
                     /locus_tag="JZ382_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428828.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKK"
BASE COUNT          998 a          377 c          431 g          721 t
ORIGIN      
        1 aagaaaaaag aattgcttgg tttataatat tcaatatcaa aagagaaaga ggatctatta
       61 atggacggta tatttggttt tactggtatt gttttattta ttataggttt aatcatgtta
      121 attgtaagat ttatcaaaaa aacaaataag aaaacaccta tgattctcct tattatcggg
      181 attattttta ctaccgtagg tttctcgctt tcctcatcaa acgaggcaaa tgacaataac
      241 aaacaggaaa gctcatcatc tgatactcaa actactacat ctaacagcag caataaaagc
      301 ctaactcaat ttgaagaatt tttagagaca aataatcatg actggggtgc tttcttagat
      361 agttattatt ctattactcc agctactgag caaaatacag cttttactaa atatattagt
      421 ggtaaaacat atacttttga aggaactgtt atcgaatcaa tgactactag aatagccatt
      481 atagcagaca aagagtacga tgataaaagt tggaatgata tttctgcaac acctaaagtt
      541 tcatatgtaa tttttgcgaa ggacttaaac aatgctgata cctttacaaa gggcgataaa
      601 gtaactttta caggagaaat tagttcaaga ggatcgaaca tagaaaaatc ctatgctcaa
      661 tgggatatga ttaatagtaa agtaagtaaa aaataaatga accacgcccc actaagagtt
      721 agattgtgca caataataaa acctatacaa aagagtgcca tcaggagtca caatgcttgt
      781 aaaggaaatg acaaaatagg aggtaactat ggataatagc aatgaaaaac taaccaaaaa
      841 agaatatctt gaattaatag ttgaaggcgg tattggagca attccttata ttggtggcgt
      901 tgtgcagtca ctttattttg ggtctaaaaa cgagaagcgt ttcaaacgca tcgagaaatt
      961 ctacaaaaat ttaaacgaat cattagaaaa cattcgcgat caaattccag aagatgtttt
     1021 caacttagag aacaaggacc aactcattgg aatattcgaa gggataaatg acgaggtaga
     1081 aaaatcaaag gcacaaaata aaatagatat gtataaaaat ctttataaga attgtttact
     1141 tcgaattaac agcgcttcat gggataacga ggagtattat cttcttgtac tgaatcaatt
     1201 aacatcgatt gagattcaat tgttagcatg cttaatgagt cgtggaaacg aaaatttcac
     1261 tggaaatatc agcatgtctg gctattcaca agagctaata gatggtagct taaatagatt
     1321 atctgatttt ggtctgttag aaaaaacgat tagctccata gtattaggtg atgtcggcaa
     1381 acaaaatatg ggctacaaaa tatctcattt aggaattcaa tttattaact caactcttac
     1441 ttaagttttc tttctaatat gacatctaga cctatcagca atgcaacaat aaaaaaccag
     1501 acataccaat aatcaacaaa taatctaaac tctggaagtt cagttattct tttaaaaatc
     1561 actttaatca taataaacac ctctgattgg atgatctaat acacatccaa ttataccatt
     1621 aaaacgacaa atagcacatc cttgcgccgg caagcaaaaa ggatgtgcaa aaaataataa
     1681 aacccaaaca atgggcttct ttatagttcc tattgtatca gagaaagaga gctgattcaa
     1741 ttatggcaag attagtcaaa cgtggaaata gttggcaata cgaaatttca tacaaaaaag
     1801 atgacggaaa atacacgaag ataagaaaat caggatttaa gacaaaagga gaagcaaaag
     1861 atgccgccaa cgaattagaa tataacttga acaaaggcct taaaggggat cgcaaaaatc
     1921 tattattatc agattacttt gaggattgga tgcaacttta taaagaagga acagtatctc
     1981 ctatcactta tagaaaatac gaagatacgt taatgaacat aaagaaatat atgccagcgg
     2041 tattgatttc tgatttagat agagtgggat atcaacgctt tttaaataag tatgctaaag
     2101 accatgtaaa atctaccgtt attaagttta ataaccatat tagagcgtcg ttgaaagatg
     2161 ccgtagaaga aggattaatt ccgtttgatc caactagaaa agcagtaatc aaaggaaaag
     2221 attcattgaa gccaaaagaa gataaatatt tagattatga tcaatttaaa tctttaatga
     2281 aactcgtaga agaaaacctt tctgcgcagt actcttctcc tatgctcgtg ttagttgctg
     2341 gtgccactgg aatgcgattt gctgaacttc taggattaac atgggaagat atcgatttcg
     2401 aagatcaaat catcactatt aataaaacat ggaattataa attaaatgaa tggggaaaaa
     2461 caaaaaacga aacttcaaat aggaaaattt ccattgataa acatacgatt gatctcttaa
     2521 aaaagtt
//
LOCUS       DADJYG010000238         2518 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.246, whole genome shotgun sequence.
ACCESSION   DADJYG010000238 DADJYG010000000
VERSION     DADJYG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.246"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..179)
                     /locus_tag="JZ382_002695"
     CDS             complement(<1..179)
                     /locus_tag="JZ382_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY2428829.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="JZ382_002696"
     CDS             complement(192..1295)
                     /locus_tag="JZ382_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAY2428830.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="JZ382_002697"
     CDS             complement(1385..2320)
                     /locus_tag="JZ382_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAY2428831.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          634 a          520 c          450 g          914 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaag
//
LOCUS       DADJYG010000239         2483 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.247, whole genome shotgun sequence.
ACCESSION   DADJYG010000239 DADJYG010000000
VERSION     DADJYG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.247"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..485
                     /locus_tag="JZ382_002698"
     CDS             <1..485
                     /locus_tag="JZ382_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAY2428832.1"
                     /translation="LALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYIT
                     NLEPEEYLTILKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNE
                     NEIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY
                     "
     gene            458..1135
                     /locus_tag="JZ382_002699"
     CDS             458..1135
                     /locus_tag="JZ382_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY2428833.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1125..2267
                     /locus_tag="JZ382_002700"
     CDS             1125..2267
                     /locus_tag="JZ382_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY2428834.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2280..>2483
                     /locus_tag="JZ382_002701"
     CDS             2280..>2483
                     /locus_tag="JZ382_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2428835.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVC"
BASE COUNT          918 a          323 c          494 g          748 t
ORIGIN      
        1 ttttagcttt gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta
       61 atagtgatgc tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata
      121 ttatttatat tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat
      181 ttatgatcgg aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa
      241 taaatttagg aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc
      301 taaatgaaaa tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa
      361 gcttgaaaaa tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa
      421 cattgaatca aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt
      481 attaaaatat actagtgatc catacattat tattggggct ggcggacacg caaaagtcat
      541 tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa
      601 taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag
      661 caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat
      721 tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat
      781 agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa
      841 cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga
      901 ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca
      961 tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg
     1021 atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct cacattcctt
     1081 agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt
     1141 tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag
     1201 ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat
     1261 atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg
     1321 ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta
     1381 tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac
     1441 ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa
     1501 acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg
     1561 aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat
     1621 cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt
     1681 catttaacgg taataagatc atcacaacct caggtggagg agctctagtg tctagtgata
     1741 aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt
     1801 atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac
     1861 gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat
     1921 ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg
     1981 tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag
     2041 ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac
     2101 tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg
     2161 tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata
     2221 gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta
     2281 tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag
     2341 aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag
     2401 ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg
     2461 aaataaattc agtttttgtt tgt
//
LOCUS       DADJYG010000240         2473 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.248, whole genome shotgun sequence.
ACCESSION   DADJYG010000240 DADJYG010000000
VERSION     DADJYG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.248"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(400..2035)
                     /locus_tag="JZ382_002702"
                     /pseudo
     CDS             complement(400..2035)
                     /locus_tag="JZ382_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          842 a          436 c          372 g          823 t
ORIGIN      
        1 cctggcatat attgcagatt agaagcatgt aattttttct cagaaacaaa ccactcattg
       61 gaaaagacat gaatcaaatc atcacttact tgtgtaaaat agcgacgttt aatgacaaag
      121 atatcttcat tgctatctag tgtaccttca tttctttgtg cttaaattca aagataattt
      181 tctcaataaa gatttttgat tccagtaaga ttcctttact ccttttacaa catagcgttg
      241 tcctttgttt tgaaacatct tcttcccctc tttgccatta aaaatagccc aattctctat
      301 aaagaagatt aagcttcatt attataatat aaagtttaaa tttggtttat tacttacttt
      361 ttcttattct ctagtaagct aatattgaaa aaaatgtaat cagatcatta tttttatatg
      421 ttgtaacttc ctgataagaa aactaattaa aagaaagagg taactacata aactaattaa
      481 aaaagctaac ttcatagttg gaggataaat ggaagaaact tcaatgattg tttctccttg
      541 tgttccttca atcgacacta gtcctattat caatctctct tttttgtcca tttacttttg
      601 cgactgttcc ccaataatca aatacaggaa gcgttatttt ttgtccttta gattcatgta
      661 ctgtatagac tattttcgaa tccatcactt gataaggcat agtatgccat tccttgccaa
      721 ttcttacttc atgattctgc aaatgttctt tgtattgtaa tgcttccgtg ggtgcatagt
      781 cataaagtcc tccgggaagg agtgtcgtgt aacgaataag attttcttga ttcattacca
      841 agtgatctct ctgagtgaca actttatgtt catagtttgc ttttgttgca gcagatacag
      901 ccaacagtaa taaaacaatt gccgttatat taactacttt tctttgctta gctgatgtgc
      961 tcttcatgaa aacgaacgat aaaatcaacg agccaaacag tacttgaaag cctaagattc
     1021 gccaaggaaa ttgaatcatt tgaatgggtg tattttgtaa taaagaccat ggaaaaaatt
     1081 tggtggataa gagccaagag actaccgcac taaggtaaat cactttttct ttgcgattaa
     1141 atcctttaaa aaagaagagt ggacagatca aagaaataat accaatgatt cctaccgtaa
     1201 aggttgtcag gtcattattc atcgcaccaa tcaaactatc cgagacattt aatgcttggt
     1261 gttgtaaggt cgttttatct ggctcattaa tcgattgaac catgatttgt cggaacatcg
     1321 gataccaaaa gtatgctgtt aaaagaatcg tcattcctac tgcctttgac aactccacaa
     1381 tcaaccggat gctttctttt tttcttgttt cacttactac aaagccgcct aagactagta
     1441 agccaacggc tattacactc ataaaagtgg aaagaacatg tgtataaatg agtaagctca
     1501 tactgactgc taatggaatt gcacatggct ttttctcata aatcagtgcg taagcataat
     1561 acataactat cggtataacc gtaagtgcaa tcgcttcagc aatagcagaa cgatgataaa
     1621 tatcaatcgt acggtaccca taaaatgtat ataaacagct aaatataaaa gccgcatcga
     1681 ttctttttaa aaattttagt ccataatgaa acatcagcat gatagtacaa acattcaata
     1741 gccaaacata taagatataa ctaacgatta aattattact tatccaataa aaaatgactg
     1801 ccggaaaaaa ggtcaagtag ggataaaaat aattcactcc gctcccataa tgattaaacg
     1861 tcgtaaaatt gattggacca gaaaaaattg aagataaccc tttgactcta gcaaaatgga
     1921 aggcaatatc ccccgatgta gaaatagtat gaacatttct aaaataattg attgaataaa
     1981 gtccagcaat aattgctagg agaatggaca atattatttt tttctttact tgcattactt
     2041 aattatatac tcctttcatg gattgcttta tctagtataa cttaaatttt aaataaatcg
     2101 ttatttattt ttactctcac ttaaaaaagc actaaaatta taagagaggg aggtaatggt
     2161 ctgaacacca agaattgttc caacatcgaa ttgcttaact aatgagttca aagcttaatt
     2221 atgtggctct tatatttacc gagcgtggat tgcgatctct ttagcgaaac aaacatcttt
     2281 cataaaaaaa acagccatat ccgctttctt ggatatgact atttactact actttactat
     2341 ctttcattct tcttgtctca cttatgataa ggcgctgcgt atcatgagtg aatgatatta
     2401 ggaaaacttc ataaaataaa tcttttatac atttccactt ctagatggtc attcatgtta
     2461 gaagaatcaa act
//
LOCUS       DADJYG010000241         2436 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.249, whole genome shotgun sequence.
ACCESSION   DADJYG010000241 DADJYG010000000
VERSION     DADJYG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.249"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            361..642
                     /locus_tag="JZ382_002703"
     CDS             361..642
                     /locus_tag="JZ382_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428836.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            644..1060
                     /locus_tag="JZ382_002704"
     CDS             644..1060
                     /locus_tag="JZ382_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428837.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1682..>2436
                     /locus_tag="JZ382_002705"
     CDS             1682..>2436
                     /locus_tag="JZ382_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAY2428838.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVR"
BASE COUNT          931 a          430 c          357 g          718 t
ORIGIN      
        1 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
       61 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
      121 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
      181 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
      241 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
      301 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
      361 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
      421 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
      481 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
      541 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
      601 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
      661 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
      721 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
      781 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
      841 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
      901 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
      961 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     1021 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     1081 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     1141 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttgaggcg
     1201 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     1261 ccctgaaaca aaaaaagact gctctcacga gcagcccaaa atgagattat cctattcatc
     1321 aacataaaaa tattacaaag tgaagaacta gagaaagaca aaaaaagcat atcaaatcct
     1381 ctcaaaaata acaagtcttt aaaaaatgac agataaaaat gcacaaagaa ctaaaaaaga
     1441 gaaatcgcgt aaaatcaagc attagaagct agtaactagc tagaccaatt aaaaatcaca
     1501 aagtgagcgt ctctatccat tggtagaggg gctacttggc atagtgtaca ttatgttaac
     1561 taacgctata aataacgatt atgttcccaa aacaatattt tatgaactat aacaaaaatt
     1621 tgttatcgca tattgtatat tttaattata ggtgtatacc acttaaattt aacacaaagg
     1681 cgtgattatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
     1741 tggaattttt agtataggaa ttacaaatac ctatgcttca gaaaattcta atttcaatat
     1801 caactcccaa ccaattgaag aacaagcaat taattttgcg aaaactgatt ttgaacactc
     1861 cataacttca attcttttaa tggaaaataa caccgaaact gatattaact atactcttgg
     1921 ttcgccattt aaaatgctcg atggagaaaa tacggcaaat agttctattt tctttccagt
     1981 attttcagac gctgataata cgattaaata tatttatacc gtcaaaaaaa atacaaatgg
     2041 agaatattca ggaagtattt cacaattttt agcaaaagaa cttcaaaatc tagtagatag
     2101 taatataaca gatacaacct tattttttca agataatagc atttattatt ccgataatgg
     2161 acaaattaaa ctactttgga aatcaccttt aagtacagaa aaaaatggtg tttcttcctt
     2221 tacagaaaaa aatgatctta tatccgtaaa tataaaaaat aaaattaaaa atttctctaa
     2281 aaaacaaaat tttcctgcgg ttaaacgtgt aaaaagtgtc tcctccggag ataactttat
     2341 tatgattaat tggcgtatag cagaaaccca aacaagtttt ccgtggtgtg ctgcttacgt
     2401 agaagctgct atcttatgta acaaaacaga tgtacg
//
LOCUS       DADJYG010000242         2435 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.250, whole genome shotgun sequence.
ACCESSION   DADJYG010000242 DADJYG010000000
VERSION     DADJYG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.250"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            108..245
                     /locus_tag="JZ382_002706"
     CDS             108..245
                     /locus_tag="JZ382_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAY2428839.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            282..575
                     /locus_tag="JZ382_002707"
     CDS             282..575
                     /locus_tag="JZ382_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428840.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            572..796
                     /locus_tag="JZ382_002708"
     CDS             572..796
                     /locus_tag="JZ382_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY2428841.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            793..1812
                     /locus_tag="JZ382_002709"
     CDS             793..1812
                     /locus_tag="JZ382_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY2428842.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            2001..2074
                     /locus_tag="JZ382_002710"
     tRNA            2001..2074
                     /locus_tag="JZ382_002710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2035..2037,aa:Ile,seq:cat)
BASE COUNT          825 a          407 c          489 g          714 t
ORIGIN      
        1 tttagcacaa atcaattcaa aaatgatgct tgcttcgtta caaaatagca aagacatttc
       61 agaaattaaa gagcaactaa atccagcttc aaaaggaggt gagtagtatg tttagtttta
      121 gcgatgtgaa aatgatgtat gattggggct gttttactga cgatcaagtt cgactattcg
      181 ttccactatg cattacagac gaagaagcag ataaaatcat taataaagat aagagcgcat
      241 cttaattgat gcgtttttat ttaaggtaaa ggagttgtca catgattaat ttaggggaat
      301 ggggaatgat agcaggatca ataaccgcta tcgtttcttt gattttatta gtaattagac
      361 cgattgctgc atctttctcg aaaattactg agactctttc aaaagtaagc cacaatttag
      421 atttgctgac taaagattta gaatcgagca aatcagatcg attgatgatt catgaagaac
      481 taaagaaaca cgatgaaaga ttagatacac atacagaaaa attggtggaa cacacgcaac
      541 aaatcaaaac tttgtttagg gagagaagaa aatgaataat aaaacgttcg aagtactaaa
      601 atggttcgca ctggtaatta ttcccgcact agctactttc gtggggttag ttggtaaagc
      661 gctcaattgg cagtacacag atatctgtgt tgtcatcatt actggttttg gcgcgttttt
      721 agggagtgtg ttgggtgtat caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga
      781 aggaggaaac aaatgaaaaa gaaaattact attactgcga tgagcctgtt aacggctctt
      841 tttttattgc caattaacgg atttgcctat actatcaaca atgaatttaa tttgggcgca
      901 aatgaaggta gctcacaagt agcaaataat cagtatattt tactgcatga aacggctaat
      961 gaaacagcaa caggacgcaa tgaagcgcag tatatgcaac gttcatggac tagcgcttat
     1021 actgcttata ttgtgggaga cggcggaatt gtttaccaag tcggacaacc tggttatgta
     1081 cagtacggtg ctggttcata tgctaatgcc aatagtcctg tgcagattga gttacaacac
     1141 acacatgata aagcaacttt tgaaaaaaac tataaagcat acgttgaatt ggctagagat
     1201 tcagcaatga aatatggtat tccattaaca ttagacacgc cttataacca accaggaatc
     1261 aaatcacatt tatgggtaac tcaaaacatt tggggcgatc acactgatcc gtatggatac
     1321 ttatcagaaa tgggtgtaag taaggaaaaa ctggcttacg atttagctca tggatttaca
     1381 gacgaaaacc caacgacttc tgagaacaag cctgtcattg atccaacccg agcaggtgca
     1441 gcaaatccaa ctttatcaga tggaacgaac cattctcaca ttgatcagtt tggagaaatc
     1501 gaaaatgcga acttacacgt cgctggatgg cacatcgcta actataagta cgagtatatt
     1561 ttcattatgg actacaatac tgggaaagaa ttagctcgag taagagctga tggaatttat
     1621 agaccggatg taaatcaagc ttataatact tctggaaatg ttggctatca tgtatctttt
     1681 aacatgcgta acttccctaa taagaaagtc tatgtcatga tgcgggcaac gaatgatcca
     1741 gagggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta tttaaatatt
     1801 cctaaacgat aaaaatagct cctcgttgag gagcattaca taactatatt gacaactata
     1861 aaaatcattc gataaaatag tgatgttatc gcatatcttc actatcaccc ataatagtca
     1921 cactccaagc tatgcgataa caggtttgtt gccacacatt ctactggttg attgtttatg
     1981 gctttatgtg gcaacaacca gtacccttag ctcagttggt cagagcagac ggctcataac
     2041 cgtccggtcg taggttcgag tcctacaggg tacattaacg tagccatttg aatcgttctg
     2101 tgttagaatt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
     2161 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatat
     2221 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttatatt
     2281 gtgagataat aataaagaag agtttaaagc gttccccaaa aaccactgcc ccataagtgt
     2341 gttacgcttt aaactctttt atatttgaag ccattaaaaa gcataccata tttttgaaaa
     2401 aaagtgagaa aaaaggctta taattggagt ggtag
//
LOCUS       DADJYG010000243         2429 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.251, whole genome shotgun sequence.
ACCESSION   DADJYG010000243 DADJYG010000000
VERSION     DADJYG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.251"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            124..2277
                     /locus_tag="JZ382_002711"
     CDS             124..2277
                     /locus_tag="JZ382_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAY2428843.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          830 a          426 c          537 g          636 t
ORIGIN      
        1 acaaaaagtt gattttatct tgaaattagg taaagaattt cttgctccgc ccattttttc
       61 ttttttatta agaagttttt gggtacaatg aagcatgaat gtaaataggc ggaaggatag
      121 gaaatgatga aagattttat gaaaaaaata ttaaatactt cttggttaga aaaaataaga
      181 aaaggtgcac aagaacctaa gaacaaaacg tttcgccggt cccatgttcc ttttcgctta
      241 aattttcttt ttttcattat atttacgtta tttgttgcgt tgattgctag attgggttat
      301 ttgcagatcg tgaacggcga agagatggag gcgcgagtga agtctacttc tacgattacg
      361 gtccaagaaa gtactcctcg cggaatgatc tacgactcca caggaaaagc tttagttacg
      421 aataaagcga atcaggccat cacatatacg agaggaacac aagttacggc taaagatttg
      481 cttgaaatcg caacgaagct gaactcgctg atcgatgttc cggtagatga aaatctgaca
      541 gatagagata aaaaagattt ttggctggct gatcccaaac atttgaaaga agccacaaaa
      601 agactttcgg caaaagaaaa gcaattaggg aacagcgaac aatatagtgc gatggtcaat
      661 aaagtcacag ctgatgagat taatttaaac gaggagcaat taaaaattgc taccatcttt
      721 aaacggatga atagtgcata tgaattaaac actgtctata tcaaaaactc tggtgttact
      781 gatcaagagt tagctgttgt ggcagaacgc gcttctgaac ttccaggagt ttcgactggc
      841 acagactgga cacgagaata taatgcagca agttcactga aaagtattct tggcagtgtg
      901 acgactgaaa agcaaggact tccagcagat gaagcagaga aatacctagc aaaaggttat
      961 tctcggaatg accgtgtagg acaaagttat ttggaaaaac aatatgaaga tgtcctgcaa
     1021 ggaaccaaaa cccaatatga agtttccctt gataatgaag gaaatgtttc gaatcaaaaa
     1081 gaaatcttct ctggggaaaa aggttctaat ttaatgttat ctatgaatgc agaattccaa
     1141 tcaaaagtag aagaaatttt gaaaaggaat tatcaaacat tgatcaacaa cggcaaagca
     1201 cagtattcgc ctggtgctta tgcagtggct atgaatcctc agacaggaga agtgctagca
     1261 atgactggtt tcagtcatga acaaggttcc aaagaaatca ccgagaatgc tttaggcacg
     1321 attactagcg cctttgatcc agggtctgta gtcaaggctg gtactttgac agcaggctgg
     1381 gcaagtaacg cgatcagcgg aaatcaagtt ttgattgatg agccgattcg tctgcaaggt
     1441 gcttcagaaa aaagctctgt ctttaatcga agcggacaag ttgctcttga tgcagtcaaa
     1501 gcatcggaat tgtcttctaa tacgtatatg atcaaagtgg cgttaaagat gttaggacta
     1561 gactatacac ctggaatggg actacctagt ttagatgaag aagctaaagc ttatcagcaa
     1621 ttgagagact ctttcaaaga gtttggtctt ggtacaacga ctgggatcga tttgccgaat
     1681 gaatctcctg ggatctcacg ttctgttgat tatatgaaga agttcaatgc ggataatggc
     1741 aaagaatggt atgctccagg taactttacc gatttggcat ttggacagtt tgacacatat
     1801 acaccaatcc aattagctca atatgcttca acagtcgcta atggcgggaa acgagtacag
     1861 ccgcacctag tcaaagcgat ctatggcaat gacgaaaatg gaaacttggg tgaagtgaag
     1921 aaagaaattg gcacaacagt agaaaatacg gtcaatattt ctgcagaaaa tatggcgatc
     1981 ctccgtgaag gattccacca agttgtccat ggtaccgatc cttacacaac agccaaacca
     2041 ttagccagtg cgaaaatgga cctttccgca aaaacaggga ctgcagaaac ggttgcggaa
     2101 ggtcatccag atattgcgac agtcaatagt aatatcgtgg catacggacc aacagacaat
     2161 ccggaaattg ccatcagtgt tgtcttgccg aacttattag acgaacaaga ccacataaac
     2221 ttgacgattg ctaaagaaat aatggacgcg tattatgata tgttcatggc aaaataaaaa
     2281 agaggtcgtg aaaaagccgc ttagtttcga gtatgaagaa aaattttgtg aaaatagctt
     2341 ctcacatttt tgcttaaatt tgacttcata ccgagaaact ggcttttgaa caccgtttac
     2401 tcggcaaaaa aggttgtgac aaaaattgt
//
LOCUS       DADJYG010000244         2348 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.252, whole genome shotgun sequence.
ACCESSION   DADJYG010000244 DADJYG010000000
VERSION     DADJYG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.252"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            157..2019
                     /locus_tag="JZ382_002712"
     CDS             157..2019
                     /locus_tag="JZ382_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2428844.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2019..>2348
                     /locus_tag="JZ382_002713"
     CDS             2019..>2348
                     /locus_tag="JZ382_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY2428845.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          844 a          349 c          459 g          696 t
ORIGIN      
        1 aaaacgcttt ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag
       61 ttaaaaagct ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag
      121 aaaacgctta tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa
      181 aggcagcttt tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct
      241 gaaaaagaaa atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg
      301 ctgaatgagc ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt
      361 agtagctctg atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata
      421 tttgcagatc aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa
      481 gctattactg gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat
      541 gatttagatg tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg
      601 cgaaagggtt tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt
      661 cttcaaaaag agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc
      721 ggtgagactg aatactttga aatgtattct gctactgcta cacctatcca agaggtattt
      781 aatcaagtaa tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat
      841 tacgcggaat tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa
      901 gaggctacga ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt
      961 tcttatcgcg tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa
     1021 tatcattata tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta
     1081 gttgagaatg tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca
     1141 caattgttga cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt
     1201 gtcaatgaat ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat
     1261 gcgatcaagc aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct
     1321 ttcgtggcat atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga
     1381 agcgttagag ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa
     1441 aagatcaatg aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg
     1501 caagaaatga ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc
     1561 atcgatcgtc ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt
     1621 caacaaataa taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat
     1681 cttagagaaa cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg
     1741 aaagcttata cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa
     1801 tatcgagaag cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa
     1861 caatatatgg cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta
     1921 ggaaaaatcg aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt
     1981 gcattattct catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt
     2041 taaaaattat cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg
     2101 aagagcgatt agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta
     2161 atcacttgaa tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc
     2221 gggatttgtt caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa
     2281 gtatcggtaa tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa
     2341 aaaataat
//
LOCUS       DADJYG010000245         2293 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.253, whole genome shotgun sequence.
ACCESSION   DADJYG010000245 DADJYG010000000
VERSION     DADJYG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.253"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            202..1077
                     /locus_tag="JZ382_002714"
     CDS             202..1077
                     /locus_tag="JZ382_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428846.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="JZ382_002715"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="JZ382_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..>2293)
                     /locus_tag="JZ382_002716"
     CDS             complement(1642..>2293)
                     /locus_tag="JZ382_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2428847.1"
                     /translation="TIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDP
                     FLIQNMQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKD
                     FAKLSVTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVV
                     QIPWTTPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT          817 a          410 c          361 g          705 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtg
//
LOCUS       DADJYG010000246         2258 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.254, whole genome shotgun sequence.
ACCESSION   DADJYG010000246 DADJYG010000000
VERSION     DADJYG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.254"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            127..693
                     /locus_tag="JZ382_002717"
     CDS             127..693
                     /locus_tag="JZ382_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2428848.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            838..925
                     /locus_tag="JZ382_002718"
     tRNA            838..925
                     /locus_tag="JZ382_002718"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Ser,seq:cga)
     gene            1259..1825
                     /locus_tag="JZ382_002719"
                     /pseudo
     CDS             1259..1825
                     /locus_tag="JZ382_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            1938..>2258
                     /locus_tag="JZ382_002720"
     CDS             1938..>2258
                     /locus_tag="JZ382_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428849.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT          810 a          351 c          423 g          674 t
ORIGIN      
        1 aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta aaatgtttgc
       61 aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag aagtggggga
      121 gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc ttatgatgtt
      181 gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa gatggggatt
      241 tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa tacgttgctg
      301 gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac tggtgataaa
      361 atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag tctttacatg
      421 gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc atacaattat
      481 ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga atatgtcgat
      541 gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat gtcatcaaac
      601 atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat tgactcgatt
      661 ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa tttgatcggt
      721 aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga tttctgttat
      781 ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga
      841 aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta ataccggcgc
      901 gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa gaagctgtcc
      961 tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt cgtttgattg
     1021 ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag gggctgtgac
     1081 aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt tttgattccc
     1141 cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt tttttgataa
     1201 aaaaaatact agatgtaaga taaaagtgta aaaacgattt gttttaggag gggaaacgat
     1261 gtgtaccgca atcacttatg caacgaaaga ccattatttt ggaaggaatt ttgattatga
     1321 gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg attttagaaa
     1381 ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg tatccaacta
     1441 tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac tgaattttcc
     1501 tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac catttgagtt
     1561 tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat tattggctac
     1621 tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt tacactggtt
     1681 attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac ttcatttata
     1741 tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat tatttaatct
     1801 gaacaattat cgctcgctat caaatggaac gagagtataa aatattttgt gtaaatgaaa
     1861 aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca
     1921 gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta
     1981 aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac acactcctac
     2041 ggactgaatt aacggctttt ttagattacg aaaaatatga tcgcactggt tttaattcag
     2101 gtaattcgag aaacggttct tactttcgat caatcaaaac cgaatatggt gaattaacat
     2161 tggaaatacc tagagatcgt aatggtgagt ttaaacaaca aactttacca gcctacaaaa
     2221 gaacaaacga tacattggaa accactatta tccattta
//
LOCUS       DADJYG010000247         2252 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.255, whole genome shotgun sequence.
ACCESSION   DADJYG010000247 DADJYG010000000
VERSION     DADJYG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.255"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..1700
                     /locus_tag="JZ382_002721"
     CDS             87..1700
                     /locus_tag="JZ382_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAY2428850.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          707 a          401 c          444 g          700 t
ORIGIN      
        1 caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata acatagtacg
       61 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
      121 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
      181 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
      241 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
      301 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
      361 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
      421 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
      481 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
      541 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
      601 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
      661 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
      721 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
      781 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
      841 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
      901 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
      961 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
     1021 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
     1081 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
     1141 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
     1201 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
     1261 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
     1321 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
     1381 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
     1441 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
     1501 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
     1561 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
     1621 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
     1681 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
     1741 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
     1801 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
     1861 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
     1921 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
     1981 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
     2041 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
     2101 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
     2161 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
     2221 taatttaatt tttttgttcc tatttattta ac
//
LOCUS       DADJYG010000248         2246 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.256, whole genome shotgun sequence.
ACCESSION   DADJYG010000248 DADJYG010000000
VERSION     DADJYG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.256"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(46..2130)
                     /gene="cadA"
                     /locus_tag="JZ382_002722"
     CDS             complement(46..2130)
                     /gene="cadA"
                     /locus_tag="JZ382_002722"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAY2428851.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          647 a          511 c          355 g          733 t
ORIGIN      
        1 ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt cgtcctcttt
       61 ttttacatgc aagcttcgca tggcattcaa aacagctaat actgtcaccc caacatctgc
      121 aaatactgca tctcccattg atgcaaaacc aaaagcacct aaggcaagta ccaaaatttt
      181 tatcccaata gcaaagacaa tgttttgttt gacgattttc aatgttttct ttgctaaatg
      241 gattgcttct gcaatcttcg atggttgatc attcatgatg actacatctg ctgcttcaat
      301 tgcagcatcg cttcccaatc cacccattgc gattcctaca tctgctcttg ctagcaccgg
      361 tgcatcgttg attccatctc cgacaaatgc ggtcttttta ttgtttcttt gtaagatttc
      421 ctctaaccgc tgcactttat cttcaggaag caattcgcta tatatcttgt ccacgcctac
      481 ttgcttcccg atatgatcag caattttttt cgagtcgccc gtcaacataa ctgtgttctt
      541 cacaccagcc tgttttaatt gaaccaatgc gtctacggca tcttctttca aggtatctgc
      601 aataacaaga taaccactgt aactttgatc gatcgctaga taaagcaatg taccgatttc
      661 ttgacttaaa ggaaaagaga tttcaaatct ttccatcaat ttgtgattac ctgctaatac
      721 ttgtttgcca tcaacttcag ctaagatccc ttcacctgca aattcctgga tattagttgt
      781 tggtaaaagc ggacctgcgt agccttctaa aacagattga gcaatcggat gtgtagaaaa
      841 ctgttctgca ctcgcaatat attgcatgaa tttctttgga tcgatttttg tatcgatcgt
      901 ctggatcgaa aagtcacctt tcgttaatgt gccggtttta tcgaatacca ctgtctcggt
      961 ttgcgcaagc aactccaagt aatttgaacc ttttactaat acaccgattt tacttgcgcc
     1021 tccgataccg ccaaagaaac taagtggcac ggaaatcact aatgcacatg gacatgagat
     1081 cactaaaaat gtcaatgctc gatagatcca ttcagaaaac gccgctccag aaacaacaag
     1141 tggaggtaaa acagctaata aaacggccaa tcctacaacg actggtgtgt aatagcgcgc
     1201 gaatttagta atgaaatttt cagctggtgc ttttttactg ctggcatttt ctactaattc
     1261 taataatttg ctgatagtag aattttcata agatttttct acttgaacta aaaatggttt
     1321 atttttattg ataaatccgc ttaatactgt ttcaccaaca tatacagatt tagggactga
     1381 ttctcctgta agtgctgacg tatcgactaa tgattctcct tcgatgatcg ttccatctaa
     1441 cggtacacgt tcaccaggct tcacaagtac aaactgaccg atctgcacgt cttcagggtt
     1501 cacggtcatc aatccttccg tcgtctgcac gtgagctgtt tctggacgga tcgccatcaa
     1561 ttcgcgaatc gattttcttg aatgactgac tgccaatcct tggaaatact cgccgatttg
     1621 ataaaatagc atcactgcta cggcttctgg atattcaccg atcaacatag cgccaatcgt
     1681 agcaacagac ataagaaaat tctcatcaaa gatttttcct ttgacgatat tcatcactgc
     1741 cttcttcact acatcatatc ctatcagcag ataaatcaat aaatacccta tcaagcgaat
     1801 tggttcatta aaatcaaata atagcaataa agcgatacct gcaagggaaa ataaaatccg
     1861 gaacagcgta tttctctctt tttcatttcc atgttcagat actttttcct tatctatttc
     1921 tgttactttg acatctggtt ctaattcctt cactactttt tttgtttctt gttcaatcgt
     1981 agccaaatga tcttcctctg cgtcaatcgt tagttttcca gaagtgaagt ttaccgttgc
     2041 ttccttgact ccattgatct tctgaactcc tttttcgatt ttcattgcac agttcgcaca
     2101 atctaaacct tctaatctgt aacttttcat tttaatgacc tcctcgcctc aacatatgaa
     2161 catctcttca taactaatta taaatgaatg gttattcata tgtcaaggta tccgaaagaa
     2221 aaaatgaaaa acatctgcat cggaaa
//
LOCUS       DADJYG010000249         2219 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.257, whole genome shotgun sequence.
ACCESSION   DADJYG010000249 DADJYG010000000
VERSION     DADJYG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.257"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..198
                     /locus_tag="JZ382_002723"
     CDS             <1..198
                     /locus_tag="JZ382_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428852.1"
                     /translation="IDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKN
                     KYQIKGIKLNQKTIKKLNIGG"
     gene            201..950
                     /locus_tag="JZ382_002724"
     CDS             201..950
                     /locus_tag="JZ382_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428853.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            962..1231
                     /locus_tag="JZ382_002725"
     CDS             962..1231
                     /locus_tag="JZ382_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428854.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1393..1695
                     /locus_tag="JZ382_002726"
     CDS             1393..1695
                     /locus_tag="JZ382_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428855.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1692..1853
                     /locus_tag="JZ382_002727"
     CDS             1692..1853
                     /locus_tag="JZ382_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY2428856.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1850..2155
                     /locus_tag="JZ382_002728"
     CDS             1850..2155
                     /locus_tag="JZ382_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428857.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          869 a          354 c          504 g          492 t
ORIGIN      
        1 atcgatcact ttgacaaagc gctaggaaga cgaaagcgca aagaagttga tcatgcagag
       61 tacacatttg cagcactctg cagaatccat cacacagaga agcacaaaat aggtgtgatt
      121 aatttcaaaa ataaatatca aatcaaaggg atcaaattaa accagaaaac aatcaaaaag
      181 ttaaatattg gagggtaaaa atgacagaac atcgaagtta ttatgcgatt ataccagcca
      241 acgtaaggta cgacaaaaga cttaaaccaa atactaagtt gttatacgga gagataacgg
      301 ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga gtactttgca gatttatatg
      361 gtgtgaataa agagaccata tcgcgatggg taagtgattt gattaagttt ggatacttga
      421 atcgggaaat catttacaaa gagggtacca atcaaataat caataggtac ctacgaatta
      481 atcaataccc tattgacgaa aaacgcaata cccctattga cgaaaaagtc aaagataata
      541 atacatctat taataataca tttaataata caaaagaata tataagagag ttaccgcctt
      601 cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg agagtataaa aatgttcttt
      661 tgtcagatga gcagatggag aaactcaaaa cagaattccc taatgattac caagagcgaa
      721 tcgaacgact gtcagagtat tgtgaatcat ctggtaagac ttataaaaac tatttggcaa
      781 ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc agtggataca
      841 agcgcacagg aagacgagag aagcttcctg aatgggcaat cgaccaagaa gcctatctca
      901 agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa gaggtggaaa
      961 aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa agggcgagga
     1021 attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg aaggaattaa
     1081 gaatgatcgt tcgagttgga gtaagctcag atactatttc gcactttata tcgatacaac
     1141 acaattaaga aatttagcat ataccaaatt actatttgtt gattgtgtta aaggcttgca
     1201 aaaacatctt aatgaacttg agcaggtgta atcagatgga cctaaagaca tttacagcac
     1261 agattgaact aatgcatcaa gaagctttaa gacaaagtgt atcgtacgaa gacaagtggc
     1321 tcaacacgtt ccatggcgga cgtgagagcg cacttgatca agtactcaaa ttactgaaag
     1381 gagaatgtcg ggatggataa gaaagcggca atgcagcgaa ttatcgaatt gacttattca
     1441 gaagattggc aaaatgacaa agaagctgct tcagaagtga tgaggcttgg aagagcgatg
     1501 tgggcagaca agagcaacaa gccaagacca cgaaaaatcg caatttggca cggtgacaaa
     1561 cttctagtga tagggacagc tgaacagtta gcaagtctca caggcttgca cgagaaaatc
     1621 gtgaggaaaa gagcaaggtg tggctacaca gacgctaaga agagaacgtt tagatacgtg
     1681 gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcagcgtg
     1741 agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
     1801 tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa tgagcgtgga
     1861 taagaaagca gcaatgaaaa gaattgctga attaaccaag tcagaatctt ggcaagaaga
     1921 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcccaa
     1981 acggagaacg ccgagaaaaa ttgcaatctg gcatgatgac cgaattttag taacaggtac
     2041 tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctag
     2101 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat
     2161 ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaaga
//
LOCUS       DADJYG010000250         2180 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.258, whole genome shotgun sequence.
ACCESSION   DADJYG010000250 DADJYG010000000
VERSION     DADJYG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.258"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            700..>2180
                     /gene="ltrA"
                     /locus_tag="JZ382_002729"
     CDS             700..>2180
                     /gene="ltrA"
                     /locus_tag="JZ382_002729"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY2428858.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKP"
BASE COUNT          858 a          336 c          455 g          531 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc
//
LOCUS       DADJYG010000251         2170 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.259, whole genome shotgun sequence.
ACCESSION   DADJYG010000251 DADJYG010000000
VERSION     DADJYG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.259"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(234..494)
                     /locus_tag="JZ382_002730"
     CDS             complement(234..494)
                     /locus_tag="JZ382_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428859.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            complement(860..1345)
                     /gene="ssb"
                     /locus_tag="JZ382_002731"
     CDS             complement(860..1345)
                     /gene="ssb"
                     /locus_tag="JZ382_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY2428860.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1348..2163)
                     /locus_tag="JZ382_002732"
     CDS             complement(1348..2163)
                     /locus_tag="JZ382_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAY2428861.1"
                     /translation="MMEKLLECIVESPYHDDFVLKGGFLIGSKYGLENRSTVDIDTTY
                     RNSKLTKEKLETVFSELTAAPTKDGIRFELKGLTETREADYYPGFQAKFIAFLENARV
                     PFKLDITSGDSIIPAALIYEHKLMFEDKTIHIPAYPTEQILAEKLHATFSFGIDNSRM
                     KDFYDLYMIPKLERIDEINLYKSVQGTFKERNNQSVFKNLYNQEMPQLMKNEGLREKW
                     KRYQQENYFAKDISFESTLTSVDSLMQSIVEQEEVERKQNFKRSQQMNQEMER"
BASE COUNT          628 a          419 c          375 g          748 t
ORIGIN      
        1 aaccttttgg tgagaggaag ttttgtctat tttgtcttgc tttgcaagac accatatctt
       61 agtgaacgtg cagcgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag
      121 aaaggttttt caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta
      181 tgtaaaacaa aaagagctgc aatcctcatt taggactaca actcttcttt tatttatatg
      241 cccatcgatc gcgtaaaatg atcgggattt ctaacataca tgctagcaat aactagctga
      301 tttcgtttat tgaagtgacc tgtgacggca atattgtatt ttccatcatc cataattagc
      361 aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg aatcgtttgt
      421 aaagtaaagc gaaccaataa cggttgcgtt tttattactc ggattttatc aagcaaacca
      481 acatatttgt tcatcacaat cacctcgcta aaattatagc aaaaatccaa tcatttggat
      541 atttcaatta tgctataata gtcttgctgt ctgctacccc ggcctaatct ggcaagggga
      601 ggaggttata ggtttgaaaa ttctttttga ttttgttttt gcaatcatgg cagaagtagc
      661 cgcgcactat atctgcaaat ggcttgattg taaatttgcc aagaaagaca gtaagcctac
      721 ccgttaattc ttataataga aacgtaaaag aaagccacta gcccgccagc tagtggtttt
      781 tggttataga ttgaaaattc tttttgttgt ctaaattata acataaaccg tagcaaatgc
      841 aaccaataga aaatgctact tagaacggta gatcatcatc gctaatatcg attgattgtc
      901 caccaaatgg attctgtgaa gcgttcaatc cattgttgtt aggcgtcgct gtatcccgtt
      961 gttcaaaggt ggtagggctt ggactgctgg acgattgatc agcgtgcata cggctttctg
     1021 tcgctgcttt actttctaat agttggaaat tatcggccac aacttcagtc acatagacac
     1081 gctgtccttg ctggttgtca taagtccgcg tttgaatccg tccgacgacg ccgatcaagc
     1141 tgcctttttt agctaatgtc gctatcgttt cagctggttt gcgccaaatc acacaattga
     1201 taaagtcagc ttctcgattg ccactttgat tagtaaaatt acggttaacg gctaaggtaa
     1261 aagtggtcac tgcagtgcca cttgcggtgt aacgtaaatc gggatctttg gttaatcgac
     1321 ctactaaagt tacattattg atcatcttta tctctccatt tcttgattca tttgttgtga
     1381 tctcttaaaa ttttgttttc gttctacttc ctcttgctcg acaattgatt gcatcagcga
     1441 atcaacagaa gttaaggtag attcgaaaga aatatctttg gcaaaatagt tttcttgctg
     1501 atatcttttc catttctccc tcaatccctc attcttcatc aattgaggca tttcctgatt
     1561 atacaaattc ttaaacactg attgattatt tctttcctta aatgttcctt gaacactctt
     1621 atacaaattt atctcatcga tacgttctag tttagggatc atatacagat catagaaatc
     1681 tttcatacgt gaattatcta ttccgaaact aaacgtggca tgtaattttt cagctaaaat
     1741 ttgttccgta ggataagctg gaatatgaat tgttttatct tcaaacatca atttatgttc
     1801 atatatcaat gctgctggaa taatagaatc acctgaagta atatctaatt taaaaggtac
     1861 acgtgcattt tcaagaaaag caataaactt tgcttgaaaa cctgggtaat aatcggcttc
     1921 cctcgtctct gttaagccct ttaattcaaa ccgaatgcca tcttttgtag gagcagctgt
     1981 taattcagaa aaaacagttt ctaatttttc ttttgttagt ttcgaatttc gataagtcgt
     2041 atcaatatca acggttgaac ggttttctaa cccgtatttt gatccgatca gaaatccccc
     2101 ttttaataca aaatcatcat gataaggcga ttcaacaata cattctaata atttttccat
     2161 catataatat
//
LOCUS       DADJYG010000252         2104 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.260, whole genome shotgun sequence.
ACCESSION   DADJYG010000252 DADJYG010000000
VERSION     DADJYG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.260"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            169..1539
                     /locus_tag="JZ382_002733"
     CDS             169..1539
                     /locus_tag="JZ382_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAY2428862.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="JZ382_002734"
     CDS             1560..1844
                     /locus_tag="JZ382_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428863.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DADJYG010000253         2081 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.261, whole genome shotgun sequence.
ACCESSION   DADJYG010000253 DADJYG010000000
VERSION     DADJYG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.261"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(544..909)
                     /locus_tag="JZ382_002735"
     CDS             complement(544..909)
                     /locus_tag="JZ382_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428864.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(985..1230)
                     /locus_tag="JZ382_002736"
     CDS             complement(985..1230)
                     /locus_tag="JZ382_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428865.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1300..1554)
                     /locus_tag="JZ382_002737"
     CDS             complement(1300..1554)
                     /locus_tag="JZ382_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428866.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1718..2041)
                     /locus_tag="JZ382_002738"
     CDS             complement(1718..2041)
                     /locus_tag="JZ382_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428867.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          774 a          358 c          368 g          581 t
ORIGIN      
        1 aaaaaagaaa gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa
       61 ctatttttac tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca
      121 actgaactga tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat
      181 cacagtgact acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt
      241 aagcatcgat gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag
      301 atcaaaagaa gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta
      361 aaccacgtca ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag
      421 tacgctttta gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct
      481 tgatctaaga tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt
      541 atattaggta agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac
      601 ttcatcgtgt tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc
      661 ttcgcatgat ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg
      721 tttttccatt tcatccatga caagctgatg atattggaat aatctataag gagaatagtg
      781 aaaaacataa tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca
      841 ttcccgatgt tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca
      901 taatcgcata gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa
      961 aaccagcacc aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat
     1021 taggtaatca acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg
     1081 aatttcttct ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga
     1141 aacagctact tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt
     1201 atccagtaaa atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt
     1261 ataatgacta tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg
     1321 tatcaaataa agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa
     1381 aacactatcg ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg
     1441 aagagtcaaa atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact
     1501 tgcgaccagt gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg
     1561 gactcctcaa cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa
     1621 tgctaggaaa ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg
     1681 ctgataagca ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat
     1741 tgatatctaa gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa
     1801 gaatgatgat cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg
     1861 catatctttt tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt
     1921 gaaaaatcat tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag
     1981 ctgctgccgc aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca
     2041 ttgagaataa ataaaagaat aacatttttt ctaaaataag c
//
LOCUS       DADJYG010000254         2080 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.262, whole genome shotgun sequence.
ACCESSION   DADJYG010000254 DADJYG010000000
VERSION     DADJYG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.262"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..177
                     /locus_tag="JZ382_002739"
     CDS             <1..177
                     /locus_tag="JZ382_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428868.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="JZ382_002740"
     CDS             190..690
                     /locus_tag="JZ382_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAY2428869.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2080
                     /locus_tag="JZ382_002741"
     CDS             787..>2080
                     /locus_tag="JZ382_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAY2428870.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQT"
BASE COUNT          677 a          283 c          464 g          656 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta
//
LOCUS       DADJYG010000255         2080 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.263, whole genome shotgun sequence.
ACCESSION   DADJYG010000255 DADJYG010000000
VERSION     DADJYG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.263"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            64..1365
                     /locus_tag="JZ382_002742"
     CDS             64..1365
                     /locus_tag="JZ382_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY2428871.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1434..2033)
                     /locus_tag="JZ382_002743"
     CDS             complement(1434..2033)
                     /locus_tag="JZ382_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY2428872.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          679 a          403 c          360 g          638 t
ORIGIN      
        1 tctttcttcg ttacaataag gaatgggaca agtaaaaaaa tgaaaaagca agggacgagg
       61 tatatggaaa aaaagcaaaa aattatttta ggagccactt ctgctttaac tatcgttatt
      121 ttaattctaa gtttgttttt tgtcatccga tcatctacaa aaaaagaacc aatcattcct
      181 gaaaaaaaaa taacagaaaa agcaaaagaa ccgacagatg ataagcaaca actagttact
      241 caagcaaaaa atctggctca aagctacgat tatgaccaag cgattgcgtt attggaaaaa
      301 gatagtaaga acggatctac tgaaacacaa cagcttcttc aaacactaaa aaaggagaaa
      361 gaacaactga tcgactggtc tgatcctact cagatctctc atgtcttttt ccacagcttg
      421 atcgtggacc cgcaaaaagc cttccactct caacaggcac aaggatacaa agattatatg
      481 gtgactgttg aagaattcaa ccgttcgatc gaacaacttt atcaaaatga ttttgtttta
      541 gttaatttga atgatttgat ccaaaaagac gaacaaggaa atctttcctt taacgggctt
      601 gcgctacctg aaggaaaaaa accattggtt ctttcacaag atgatgtcag ctattatgaa
      661 tatatggata attccggttt tcctagccga ttggtcgtat caaataacaa tgaaatcaaa
      721 aatgtctata aagagaaaga taaagaagaa atcggtgact ttgacatggt tcctctgatc
      781 gatgcctttg tcaagaaaca tcctgatttt tcttatcgag gcgcaaaagg taccttggcc
      841 ctgacaggtt acaacggagt tttaggttat cgaacatcaa aatctcaata tggagacaat
      901 gaaaaaacaa aaaaagagat taaagaagct aaaaaagtgg cggaacagtt gaagaaagac
      961 ggctggagct ttgcttccca tacatggggt cacttgaata tgactcaagc ttcactagaa
     1021 gatgtaaaaa aagacaatga attgtggcag caagaagttg cacctatcct aggaaaaaca
     1081 aatattctga tttatccatt tggtgcagac atcagcgact ggcagcctta ttcagcagaa
     1141 aatcaaaaat ttgcttactt gaagagtcaa ggtttcgata ttttctgtaa tgtcgatgct
     1201 tctacaccag cttggggaca attaggtacg gatttctatc gaaatgctcg gatcaatatc
     1261 gatggcattc gttttcaatc tgatttagac ggaaaaaatc ctattcttga tccattcatc
     1321 aatgtccatg atgtctatga caaagaagca cgcgaatctg cttgatttta cgtcatttat
     1381 gaaccaaaaa agccgaactg acattttatc gtcagtttcg gcttttttat gctttatttc
     1441 ttcttcggtg cgattccctt gatcaggact tctgtccatt ctgctgtata tggaagcagt
     1501 tttgcgtata tctccggctt agaaattcct tctaccattg cttgaaaata gaggatcaag
     1561 aactcatccg aatatttcaa atccactttt ccttctttcc gccctcgatg gaaaagatca
     1621 agcatgatac tgaaactttc ttgcgtatac gcatccatcg cgtcagaaaa ttcttttttt
     1681 tctttttttg tatagtatgt catcaaatcg atataaaatt gtttgttcac tcgattgaga
     1741 taacggattt tgttttcact catggcaatc agtgtttctt caaatggaag attttccgca
     1801 atcaacgctt tcgcttcttg ccctaaatca ttcataaatg caaaaaatac ttcataaatc
     1861 agcccttcct tgctgccaaa atatttaaaa atcgtcgctt tacttacctg agcgttctca
     1921 gctaccttat ccattgtcac tccagcggtt cctatgtctg tgttcatcaa gtcaaaagct
     1981 gcttgtaaaa tctctttctt tttctcttgt gctctctttt caaaaccgtt catttgtaga
     2041 gcctcccgta attttaaact tttataatat gtatagttca
//
LOCUS       DADJYG010000256         2078 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.264, whole genome shotgun sequence.
ACCESSION   DADJYG010000256 DADJYG010000000
VERSION     DADJYG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.264"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(243..464)
                     /locus_tag="JZ382_002744"
     CDS             complement(243..464)
                     /locus_tag="JZ382_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428873.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="JZ382_002745"
     CDS             complement(562..807)
                     /locus_tag="JZ382_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428874.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="JZ382_002746"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="JZ382_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          707 a          361 c          300 g          710 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctg
//
LOCUS       DADJYG010000257         2003 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.266, whole genome shotgun sequence.
ACCESSION   DADJYG010000257 DADJYG010000000
VERSION     DADJYG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.266"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..378
                     /locus_tag="JZ382_002747"
     CDS             <1..378
                     /locus_tag="JZ382_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY2428875.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="JZ382_002748"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="JZ382_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY2428876.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="JZ382_002749"
     CDS             1859..>2003
                     /locus_tag="JZ382_002749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY2428877.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DADJYG010000258         1999 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.267, whole genome shotgun sequence.
ACCESSION   DADJYG010000258 DADJYG010000000
VERSION     DADJYG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.267"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..272)
                     /locus_tag="JZ382_002750"
     CDS             complement(<1..272)
                     /locus_tag="JZ382_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2428878.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="JZ382_002751"
     CDS             complement(244..1443)
                     /locus_tag="JZ382_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428879.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..>1999)
                     /locus_tag="JZ382_002752"
     CDS             complement(1573..>1999)
                     /locus_tag="JZ382_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAY2428880.1"
                     /translation="LLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVG
                     SYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVL
                     PKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT          629 a          399 c          333 g          638 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacct actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataat
//
LOCUS       DADJYG010000259         1976 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.268, whole genome shotgun sequence.
ACCESSION   DADJYG010000259 DADJYG010000000
VERSION     DADJYG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.268"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..425)
                     /locus_tag="JZ382_002753"
     CDS             complement(<1..425)
                     /locus_tag="JZ382_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAY2428881.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="JZ382_002754"
     CDS             complement(534..1565)
                     /locus_tag="JZ382_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY2428882.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          609 a          326 c          341 g          700 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agattt
//
LOCUS       DADJYG010000260         1948 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.269, whole genome shotgun sequence.
ACCESSION   DADJYG010000260 DADJYG010000000
VERSION     DADJYG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.269"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            239..805
                     /gene="amaP"
                     /locus_tag="JZ382_002755"
     CDS             239..805
                     /gene="amaP"
                     /locus_tag="JZ382_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAY2428883.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            818..1006
                     /locus_tag="JZ382_002756"
     CDS             818..1006
                     /locus_tag="JZ382_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAY2428884.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1019..1576
                     /locus_tag="JZ382_002757"
     CDS             1019..1576
                     /locus_tag="JZ382_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAY2428885.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1608..1850
                     /locus_tag="JZ382_002758"
     CDS             1608..1850
                     /locus_tag="JZ382_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY2428886.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          706 a          302 c          402 g          538 t
ORIGIN      
        1 atctttgcaa tttttaatct tgaataatat cctttaagta tagccagcaa tcaaaagtaa
       61 atagagaata agtaagcaat tgttatttca gtatcagact ggcaataaaa cgccgttatg
      121 atccagcaat gtttggtagc gctttattta ctgatgctat gctaactgtt cgctatctat
      181 actgtgattg tcggtagagt tatttacagg gtaaaaataa gtttatagga ggtggaaaat
      241 gaataagggg ttaaaaataa tcggtgttat catgagttta ctgctacttt cagtatttct
      301 agcgattgcg ttgatcaatg cgccgtatat tatgcctgaa caattggaac gatttcgctt
      361 ctttacttta acgaattact acatgcaaca atatattttc tgggcagctg tagtatttgc
      421 cgttcttgct attatcatct tgctagtcgt tcttttctat ccaaagtcaa gaggaacttt
      481 cgttatgaaa cgggaagatg gcaaactaac aatcgacaaa aaagcgatcg aagggttggt
      541 acgttcccat ttacacgaag aagagttcat tcattcacct aaagtacgga tacgttcaac
      601 gaaaaatcga atccatatcc atgtcaacgg agacttgaaa cgaacttctt cattagtagg
      661 gaaaacaggt gtattgatgc aagatatcga agaagaagta actaaagtac tcggaacaaa
      721 agaaacggtc aaagtggcag tcacatacag cggttatgaa gaacaagaag atacaagaga
      781 ctacaagcac tctcgagtgg aataggaggg ccaaataatg aaagaactat tttcagcata
      841 caagctccct attatctttg ggggtcttgg actactgcta gctgttttat ggttaacgat
      901 tggatttttc aagacattgt tgatcataat catgacagca attggcgcag gaatcggttt
      961 ttatttgaaa cagtcaggaa tagttgatca attttttaaa tcataggagg aaacaaagat
     1021 ggaaaacaaa aacatgaata caacaaatgg atcagttttg gcaaatgaaa aaggagaaaa
     1081 ccgcatgatg aatggtgaat tgacttatga agacaaagtc atccaaaaaa tcatcggtta
     1141 tgcattgaaa gatgtagatg gtctattgac attagatggc ggatttttct caaatatggc
     1201 tgaaaaatta gtcaacacag ataacgtaac agcaggtatc aatacagaag taggtaagaa
     1261 acaagtagct gtggatctag atgtgatcgt agaatacggt aaagacatcg aagatattta
     1321 caaccaaatc aaagaattga tcagtactga agtaaacaat atgacacatt tagatgttat
     1381 cgaagtgaat gtgaatgttg cagacatcaa aacacaagaa gaataccaaa aagatcaaga
     1441 aacagtacaa gacaaagtaa cagaagcagc aaaatccaca ggacaatttg cttcacgcca
     1501 aacagataaa gcaaaatctg ctgtaggcaa aggtgcacaa aaagtcaaag aaaataacga
     1561 accacgtgta caataagaag taagaagtca gttaggagga atttaaaatg ggatttattt
     1621 gggcattaat cgtcggcggt atcattggag caattgcagg agcaatcacg aataaaggat
     1681 catcaatggg gatcatcgca aacatcattg ctggtttagt aggttcagca attggacaag
     1741 ctttactagg aacttgggga ccaacaattg caggaatggc tatcattcca tcattgatcg
     1801 gtgcaatcat tttagtatta gtcgtttcat tctttgcacg taaattataa gcaaattgaa
     1861 gaaaaactta tgttgaaatg aagaacaaat ttgagaaccg tagcgacact cgctgcggtt
     1921 cttttttata tattttatat gaagtatt
//
LOCUS       DADJYG010000261         1937 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.270, whole genome shotgun sequence.
ACCESSION   DADJYG010000261 DADJYG010000000
VERSION     DADJYG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.270"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            160..774
                     /locus_tag="JZ382_002759"
     CDS             160..774
                     /locus_tag="JZ382_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428887.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(939..1763)
                     /locus_tag="JZ382_002760"
     CDS             complement(939..1763)
                     /locus_tag="JZ382_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428888.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          648 a          322 c          286 g          681 t
ORIGIN      
        1 aaattcataa aatatatttt taatgtcata atcgtttaaa ttttcttgat tgacatttat
       61 ttacaaaaaa tattgccatt taaaaagaaa agtgacatat ggcttcctcg tgtatgatta
      121 gggtgtattt taaatagttg cagaaagtag gtaaaagttt tgagagttgc atacatacgt
      181 gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag aaatgaaaaa attcggtgca
      241 gagagaattt tcatcgagaa acaatctggc gcaacgatta ctcatcgacc tgttttccaa
      301 gaagcattag attacgtccg agatcaagat atttttattg tggaagctat tgatcgactt
      361 ggaagaaatt atgacgagat catcgcttca gtcaattact tgaagaagaa aaatgtgaag
      421 ctgatcatta ctagtttacc gatcatggct gaagcaattg gcaatcctct cctggataaa
      481 ttcattaagg atctgatcat ccaaattttg gccatgatcg ctgaacaaga acggactgaa
      541 tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag caaacggcgt ctacaaaggg
      601 cggcccaaat tatacagtgc agacgcaaaa gatccccaac gtcgccttgt ttacaaaagc
      661 atcgttgaag atcttaaaaa tggtgttgcg attgctaaga ttgctaaaga ctacaatgtt
      721 acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg tcaatgacaa atagttgatc
      781 atgctgcttt tattatcttc tcatctttaa tttcctacat ataattacta tctataaaat
      841 taagtcttac tcagaacaga taaagcataa aattccaaca aaccattttt tacttaacta
      901 aataaagagt ctgttaatat aatcttcact tatttacgtt aatcccctac tgtatttgtt
      961 ggttccttat ctatattttc tacaaaatcc ttattatctt gtgatttagc ttcgtataat
     1021 tctttgtatg tcgacacatt ggttatacta attcctatag ctagttcttg agactctact
     1081 tttctctcta gctcctttga caaagtgatt tgagatttat ttggttccgt tggagtcaat
     1141 tctatagatt cttcataaaa atctctatac ataaagctat cattaatttt ataaaaaaaa
     1201 ttaaatgttt ttagcactgc catataacat tcaaaacatt cttctatagt tggtttactt
     1261 ccttgaggag aatgaaccaa tttattacgt ttttctatga aatttttaat ttcctttctt
     1321 atttctttcg gaaactcaat attaaagtac cctttaaaca tgctccctaa taatttgtct
     1381 acagggggag attgtaaatt ctcaaatatt agagttaaat caggtttctc ttgttgataa
     1441 aattccttta cccctatttc acaggctgta attatcgaaa taaacctatc tctagtattt
     1501 gatatatttt tacttctttt aaggagtata agagaaaagg ggatactttc gtgtccatgc
     1561 atgtaattaa ctaattgttg agcttcagaa gttatatctc taattatata gttaacggaa
     1621 ttaggaagtg tctttaactc taatgaaatt tctatccaat tactatcttc taatctgtat
     1681 atgctcgaag tataatttga ttcgtcagca agcatcgcta catcccaaac actgatcata
     1741 aaatctttta aacgttcaat catttcagca atattgctaa aatcagctaa tttttcttta
     1801 gaataaaaaa aacatttata tcagaatcaa taaattctat tttttttatt actatatcgt
     1861 catcattatc ttgaccattt atgtttattg ttccctgagt ggggagtact tctttcaaat
     1921 tcaagttaaa tttatat
//
LOCUS       DADJYG010000262         1931 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.271, whole genome shotgun sequence.
ACCESSION   DADJYG010000262 DADJYG010000000
VERSION     DADJYG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.271"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            79..1545
                     /locus_tag="JZ382_002761"
     CDS             79..1545
                     /locus_tag="JZ382_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2428889.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          593 a          331 c          396 g          611 t
ORIGIN      
        1 acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa caaattttta
       61 tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt tgcgttaatt
      121 gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca gcaaggagta
      181 tcagttgtca cctcttggct attgattttg gccatttatt ttattccata cgcattgatc
      241 gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc atgggtcgaa
      301 aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg ggtcgtccat
      361 attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg ggcagtccaa
      421 ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat ttcacttatt
      481 atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa agtgattggc
      541 ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt agctgtaacg
      601 gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca agtgaaaaca
      661 tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt atttgcagtt
      721 ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc aaaggaattt
      781 cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt aggctcagtc
      841 gcaatgggta tgctgtttgc tagtgataat attcctaaag acttgatggc aaatggggca
      901 tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt gatgatcatt
      961 tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat tgatgcacca
     1021 ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct acgtaaacgt
     1081 aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt aggaatcatt
     1141 attttgttgc cgatttttgg tatcaaagat atgaatgaat tagtaaaatg gttgacgaac
     1201 ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata tatgatgttg
     1261 aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc aaaaatcggt
     1321 tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct aggaatggtg
     1381 ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt tacgaatatc
     1441 atcacaccaa tcgtcttgat cttattaggt atgatcttgc cagcaattgc tcgtcgagac
     1501 aaaaacaaaa aaatcactga accagaaaca gtggttcagc cttaaaatag aaatacttgt
     1561 tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
     1621 gcttctcaaa tttttgtgaa gtttcggctt gtttttcgaa gaagctactt ctggaacacc
     1681 gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
     1741 atagcccctc atatttttcg tttggctggg cttattaccg cagatgcaag ctttggaaca
     1801 ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag aaacaaacgg
     1861 aaaaatagct cctcatattt tgccgctttt tgagctaatg ctgaaggtca gctgattgaa
     1921 caccatttat t
//
LOCUS       DADJYG010000263         1900 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.272, whole genome shotgun sequence.
ACCESSION   DADJYG010000263 DADJYG010000000
VERSION     DADJYG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.272"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(618..908)
                     /locus_tag="JZ382_002762"
     CDS             complement(618..908)
                     /locus_tag="JZ382_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428890.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1116..1808)
                     /locus_tag="JZ382_002763"
     CDS             complement(1116..1808)
                     /locus_tag="JZ382_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY2428891.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          514 a          365 c          296 g          725 t
ORIGIN      
        1 taaatacctt atttgatgca aaagaaatct acgatagtca acaaaaaaat tttcattata
       61 tccagaaaaa tttttcttag ttaaggacct atggattgca gtaatgcaga attcgtcgaa
      121 taaactgcca aagacataca atcatattgc ttttaaaatt gacgaacaag atatagattc
      181 gtttgtctca aaaatccaaa tgttaggttt aactgtcgaa ccaggacgtt ctcgtgtgaa
      241 aggcgaggct tcttcaattt acttttacga ctatgataat cacctcttcg agttacatac
      301 cggtactctc caggaacgtt taaaatccta caattccact acctgagact agtaatgact
      361 agtctctttt tatggcctct gatcccccca ttgagcgaat actaataggg cttcgaattg
      421 atctgttcaa tctttaagca atatccctca aatgagtttg gaaagtttag tgattcatca
      481 caaatactta aatacatgta gttatatata gattgaggtt cttgtgagcg tatgctcgtg
      541 tgggttcaaa tcccatcgtc tgcatccatc tttccaaaac aagcggctag gacatttttg
      601 tctcagccgc ttgttttcta gtttattctt tcatcttgtc ggaccttttt tcttgggtat
      661 agacttttct catattctac cacaattttg ggtttcccta acacatgata ggatctcaca
      721 acaaattgct ttctattatt ataataacct gtaacgacta acttcatccc ttcgtctact
      781 tcagctagga agttcaaact atgggaagca atcaaacaat tgaccccatt taaactgaaa
      841 cggatcaacg gtctggcact aacttgcaaa gtacggatct tatctactgt tccatatttt
      901 ttctccataa ccttcacctc aataccatta tacgaacata cattcccttt gtaaagaaga
      961 aactctgttt tatctttttg actttttgtc tattaccttt cttaaaatca tattggcact
     1021 tcctgcgaag atgaaggaca tgtactaaaa aaaagaggca gcagtataga ttactgttgc
     1081 ttctttacta atttaggttt acttgttaag aaagattatt tacgcaaatg ttcttcaaca
     1141 atttgctgag atttatcagc tgtatcttcc acttcatctt ttgctttttc taacttttga
     1201 atatattgtt tcaagcttga gttttcagca gttgcttcgt ctaatttctt ttgtgtatct
     1261 gttaactgag tttctacatt tgttttttct tgtgtcagtg ttgcgatttg ttgattcaaa
     1321 ccatttacag tattttgtaa ttctgaaagt tttgcttgtg cttcttgtag tcttgctgtc
     1381 caatctgcat tcagatgatt gatttcatca tttttttggt tgatctcatc tagtttgaca
     1441 ttgatttcat tttgtttgct ttgtagttgt gcatccttgc tgactaattg actttctaag
     1501 ttttgttttt ctgctgccga ttgatttttt tggttttcta attcatttac ttttgtttgg
     1561 tattcttgga catctgcctt tgaatcttgc aatgtctgtt ctttttgtgc tagtcgagcg
     1621 gtaagatctg taagcgcttg ctctttatct gtcaatgtat gatcaagctg tgtcagattc
     1681 tcattgattg tatcaatatc tgtctctcca ccccacaaaa taatttcatt tgcgtaccgc
     1741 gtgcctgtgt agccaattag acatagaaat gcaacgacaa tcccaccagt aataaatttc
     1801 tttttcattt gcatagctcc ttaatccaat acttttacat gctaaaagta agtgatctca
     1861 aacacaatgt caagcgtttt taaattacat gtgtaatttt
//
LOCUS       DADJYG010000264         1899 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.273, whole genome shotgun sequence.
ACCESSION   DADJYG010000264 DADJYG010000000
VERSION     DADJYG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.273"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            28..507
                     /locus_tag="JZ382_002764"
     CDS             28..507
                     /locus_tag="JZ382_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2428892.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            600..1762
                     /locus_tag="JZ382_002765"
     CDS             join(600..876,876..1762)
                     /locus_tag="JZ382_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY2428893.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
BASE COUNT          671 a          347 c          379 g          502 t
ORIGIN      
        1 gtacaaggaa tagaaagagg tgtttatgtg gttgataaaa ttcagtttaa tcaagttata
       61 gaagaattca tcaaagtaaa taatttaatg acccatattc aaaaacgacc aatcagaata
      121 actaatgatc taaaactcag tacgagtatg attcatttga ttgatgtgat tggaaattat
      181 cctgaaacta gtattacgga attagctgat cgattaggcg ttaccaaagg agctgtatcc
      241 caacagattc ccactttaga gaaaatgaat cttataagca tttctcaaaa aaaaggaaat
      301 aagaaaacta aacttgtatc gttaacaaaa tatggtcgaa aggtgtacga atcacataat
      361 tatttacatg aagagcttta tagctcaata aatgaagcgt tagaatcatt ctcaccagac
      421 caaatttcaa ccattaaaca gattctagaa aaagtatctt atacaatcaa tgaatatcaa
      481 gtaagtctga caagaaagga agattgacat gaaaaaaatg atttttgaat cggctacact
      541 agactagaca gaaaaaataa ggtgtgtaaa ataaatgaaa acacactgga ggaattttta
      601 tgtcaagacg tcaacgtaga acctattcaa aagaattcaa acaacaagtc gtcgatcttt
      661 atctcgcagg aaagccccga gctgaaatca ttcgagatta tgagctgact acctcggcct
      721 ttgataagtg ggtcaagcaa gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga
      781 tccctgaaca agtagaacta attgcattac gaaagaaaaa taaacagctt gaaatggaga
      841 atgacatttt aaagcaagcg gcgctgatat tgggacgaaa agacaagtaa ttgataccaa
      901 taagcacaaa tactcaatat cagcgatgtg taaagtgtta aacatctctt gccaaaccta
      961 ttattatcaa gccaaaccag ttgaagatga atcagaactg gaagaactcg ttcaagaaga
     1021 atttattcgc aatcgaaaag cttatggtac caggaagctg aagcagtgct tggccaatct
     1081 aggtcttcaa gtgagtcaac gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc
     1141 aacatacaca ttggctcatt tcaaattcca ttcttctact tgtaacgaag ccaagaccgc
     1201 caatattcta gacggaacct tcctacagga aaagtcatta gaagcgattg taaccgacct
     1261 tacctatgtt cgtgttggga agaaatggca gtatatctgc ctgattcttg atttgtttaa
     1321 tcgagaaatc atcggctatt catgcgggga gaagaaagat gcgacgttag tcaaggaagc
     1381 ttttgcaaag attccgtact cgttgaataa tgtacaactt tttcacacgg atcgtggaaa
     1441 agaattcgat aaccagacga ttgacgaaat tttatgggcc tttaatatca cgcgttcctt
     1501 aagcaaaaaa ggctgcccct atgataacgc cgttgcagaa tccacctata aatctgtcaa
     1561 agtagaattc gttcatcaat accgctttga gacattggct aatttgcgat tagagctatt
     1621 tgattacgtc cattggtgga ataccatccg gctgcacggc acgttaggct atgagacacc
     1681 gatcagtttt cgacagcaga gattggcgca gcgaatcctt gataatgagc tcggatgtga
     1741 taccggtggc gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg
     1801 aggaggctcg ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata
     1861 atttttgtca aaaaaagtgt tgccattcca catatatct
//
LOCUS       DADJYG010000265         1895 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.274, whole genome shotgun sequence.
ACCESSION   DADJYG010000265 DADJYG010000000
VERSION     DADJYG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.274"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(1..859)
                     /locus_tag="JZ382_002766"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="JZ382_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="JZ382_002767"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="JZ382_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY2428894.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1625..1882
                     /locus_tag="JZ382_002768"
     CDS             1625..1882
                     /locus_tag="JZ382_002768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428895.1"
                     /translation="MSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEILAIYG
                     YSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
BASE COUNT          570 a          356 c          368 g          601 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtat
//
LOCUS       DADJYG010000266         1839 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.275, whole genome shotgun sequence.
ACCESSION   DADJYG010000266 DADJYG010000000
VERSION     DADJYG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.275"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            31..840
                     /locus_tag="JZ382_002769"
     CDS             31..840
                     /locus_tag="JZ382_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAY2428896.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            880..1377
                     /locus_tag="JZ382_002770"
     CDS             880..1377
                     /locus_tag="JZ382_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY2428897.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1762..1835)
                     /locus_tag="JZ382_002771"
     tRNA            complement(1762..1835)
                     /locus_tag="JZ382_002771"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1799..1801),aa:Ile,seq:cat)
BASE COUNT          638 a          333 c          351 g          517 t
ORIGIN      
        1 agatatgtaa aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt
       61 accttgcttg atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt
      121 gatgaaaatg gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat
      181 attttttccg aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct
      241 gaaatgaagg atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca
      301 ctgagaaaag ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa
      361 acccaagatg atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat
      421 gaacatttca atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct
      481 tctcttagct atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta
      541 caaagagatc tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa
      601 atcacgccta caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga
      661 atcgaatcga gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat
      721 tacagcgggt tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac
      781 caagtaacac tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa
      841 aaagaataaa atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg
      901 caaaatcata ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca
      961 gaacgtattg ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca
     1021 cgggatatac cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc
     1081 cctcttcttt ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca
     1141 cctgaaactt tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag
     1201 atcgaagtag tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag
     1261 agtgcttttc ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac
     1321 ggagaatccg ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa
     1381 tatctatctt catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag
     1441 tcctacgtgc tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca
     1501 ggctcacgac ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca
     1561 aacggcaaaa tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc
     1621 tgatttcaca acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac
     1681 agccaagtaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
     1741 cagctttttc acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat
     1801 gagccgtctg ctctaaccaa ctgagctaaa ggtacatca
//
LOCUS       DADJYG010000267         1796 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.276, whole genome shotgun sequence.
ACCESSION   DADJYG010000267 DADJYG010000000
VERSION     DADJYG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.276"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            581..913
                     /locus_tag="JZ382_002772"
     CDS             581..913
                     /locus_tag="JZ382_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAY2428898.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1532..>1796
                     /locus_tag="JZ382_002773"
     CDS             1532..>1796
                     /locus_tag="JZ382_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAY2428899.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSV"
BASE COUNT          618 a          252 c          380 g          546 t
ORIGIN      
        1 aatacgcgaa gagagataat ttcattattt aaacaagcta aaaatcttga tattacttct
       61 tttcaggcaa tacgaatatt attcgcttac attttttgta taaatttaat tgtctgctat
      121 gcttgtgatg aattgatttt cgttctgcac ttttggatga taaatttgga caattgaaaa
      181 tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc agcgttcata
      241 cacggcatta agatttggcc cttaatgcgt taacctttca gtgttacggt attacccgca
      301 atggatttag tataacagat attcaaaagt gataccactt tttttggctg tgttaaggtt
      361 aaaaatacgt gatattacga tgaatctatg cctaagcaca atgttataaa aatgtccgtg
      421 tggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc
      481 ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat aacaagaaaa
      541 acttgttaaa aatatttaaa gaaattgggt ggtttatttt atgtcatata caggaatttt
      601 gagtctcaaa gatatttgtc actacggtaa acggtgtacc gctacagaga aaatcacgaa
      661 gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa
      721 agacaaagtc tcagaggaaa tgatttatta cattgggaaa caaaagcaaa taattttaaa
      781 agatcctatc cccttaaaag agctatatcc aacaatcaaa catgtttatg accaaaacgg
      841 tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag ggatggggcg
      901 tttgatcggc tgaattgatc attctcataa cttgtatttt atcgtttttc gcttgcttga
      961 aaatattgtc gagacttcta aaggaactta gagtcactag aaaaataagg agtagacttg
     1021 atctatcaac aattcccttc ggggagtgac ggggtaggtt ttaacgattg gtaacgtaaa
     1081 aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat ttgtgtaaat
     1141 aataagcgca gagtaaacaa gagggaattt agaagttaga caagttagaa tagcgtctag
     1201 attaatcatg agcagctact aagggatcta gactttgacg caaactcatg gaataaggag
     1261 taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt
     1321 agatacttgc gaggacgttg attattggat caatcgagca tttaccgctg tctactaggg
     1381 tttctcgttt cggacgccat aaacgagtcg aaatttttta tgtattttta agtaaacgaa
     1441 aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa attgatataa aacatagaga
     1501 aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat tatgatccga atatacgatt
     1561 gggcacacat actattaagg tttctttcca acgttgggat tacaaaggat ttgtgacgtt
     1621 tagaagaggc ggaaattgta aaggattaga tgttttagca ttagacgagg atgatttata
     1681 cgatcaaaca ttgactgata atccaattgg attcggcttg ctacctgaag atgatgaagg
     1741 taatgaatgg tttaaaatga cgcttatgaa tgataatgga gacgaattat ctgttg
//
LOCUS       DADJYG010000268         1777 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.277, whole genome shotgun sequence.
ACCESSION   DADJYG010000268 DADJYG010000000
VERSION     DADJYG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.277"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            178..375
                     /locus_tag="JZ382_002774"
     CDS             178..375
                     /locus_tag="JZ382_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428900.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            481..963
                     /locus_tag="JZ382_002775"
     CDS             481..963
                     /locus_tag="JZ382_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428901.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1021..1617)
                     /locus_tag="JZ382_002776"
     CDS             complement(1021..1617)
                     /locus_tag="JZ382_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAY2428902.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          556 a          272 c          356 g          593 t
ORIGIN      
        1 ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa cgaaaacagt
       61 acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg agttaaatct
      121 ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag gattttgatg
      181 acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg ttttacaaaa
      241 acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa agttgcccaa
      301 tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt tgcaaaatat
      361 gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc aatttctttg
      421 agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag gcgagttgtc
      481 atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt tttatttctt
      541 tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt ctacttggtg
      601 atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc tacagcagat
      661 gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt ggacagtaga
      721 ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt tcctttttct
      781 gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa aatgaatttt
      841 ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga agaattagtt
      901 ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat tgatccagaa
      961 taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt tcaaataaaa
     1021 ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc cagccggtgt
     1081 ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga tattccattc
     1141 ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc ccaatccttt
     1201 atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt ctgaccaatc
     1261 tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca cgtcagtatc
     1321 tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt tttgtgtatt
     1381 tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt cccaaagttt
     1441 ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga aagcagttgg
     1501 gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa gtgaaacatt
     1561 acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag ttgtcataat
     1621 aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa tgaagtatag
     1681 caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag ttcaaaaaac
     1741 aattttaata agaaaaatcg ttatgtaagg attttct
//
LOCUS       DADJYG010000269         1726 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.278, whole genome shotgun sequence.
ACCESSION   DADJYG010000269 DADJYG010000000
VERSION     DADJYG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.278"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..836
                     /locus_tag="JZ382_002777"
     CDS             24..836
                     /locus_tag="JZ382_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAY2428903.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            833..1633
                     /locus_tag="JZ382_002778"
     CDS             833..1633
                     /locus_tag="JZ382_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY2428904.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          525 a          273 c          396 g          532 t
ORIGIN      
        1 ttttaagtat aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac aaatatattc
       61 aagaaaaaag cgaatttcaa tttaataccg atggaccaat tcctcagcac attgccatca
      121 tcatggacgg caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt gctggccata
      181 aagaaggcat ggaaacagta aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag
      241 tgttgacgct ttatgccttt tcgactgaaa actggaagcg accaaaagat gaagtaagtt
      301 ttctcatgca actacctgtc gacttctttg atacgttcgt accagaattg attaaagaaa
      361 atgtcaaagt tcatgtcatg ggttatgaaa acgtattgcc agaacatacg caagatgctg
      421 taagacgtgc aattgagcaa acaaaaaaca atactggaat ggtattgaat tttgctttga
      481 attatggaag cagagcagag atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa
      541 aaggagaaat acacgcagaa gaaatcgatg atgaattgat tgctaagcat ttaatgacag
      601 gtttcttgcc taaagagtta caagatcctg agctgatgat ccgtacaagt ggagaagagc
      661 gaattagtaa ttttctgtta tggcagatcg catatagtga gctgtatttt acaaaagcgc
      721 tttggcctga ttttgatggt gcgcatcttg aagaagcaat cgcctcttat caaaatcgag
      781 accgtcgatt cggcggtgta aaaaaaacag aagatgagaa aggggatcag tcatgaaaca
      841 acgtgtggtc acagcagccg tggcgctagt cttgtttatt ccgattatat ggatcggagg
      901 catagcggtt gagcttacag cagcaatctt agcagtaata ggagtttacg agctgtttcg
      961 gatgaaagga ttgacactct taagttttga aggagtttta tccgccgttg gtgcagtgtt
     1021 acttgttttg ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa gtacatttat
     1081 gttattttat cttatagtta tgattctttt aggaacatca gttatttcta aaaatacgta
     1141 tacgattgat gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca
     1201 gaattttgtc aatgcacgaa cagaaagctt ggctgttttg atatttggtc tatttattgt
     1261 ttgggcaact gatatcggag catatatggt tggtcgacgt tatggtcagc ataagttaag
     1321 tcctgatatt tcgcccaaca aaacgattga aggtgcgctc ggtggtattg gtagtgctgt
     1381 tgtagtcgca tttctttatt tattgctata cccagcaaaa gaactattta atcatggtat
     1441 gtttgtcatg cttctgatga cgattatttt ttcagcagtc ggacaatttg gtgatttggt
     1501 cgaatctgca atcaaacgtc attatgatgt gaaagactcg ggaaatatct taccaggcca
     1561 tggtggaatc ttagaccgtt tcgacagttt gctgtttgta tttcctatca tgcatttatt
     1621 tggcattatt tagtaagtat acctgcgttt ctttgtgaat ttcggctctt tgtcaaatga
     1681 tgttggtaaa gagaaatctt agaataattt ggacagaaca gaaaga
//
LOCUS       DADJYG010000270         1722 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.279, whole genome shotgun sequence.
ACCESSION   DADJYG010000270 DADJYG010000000
VERSION     DADJYG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.279"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            476..1606
                     /locus_tag="JZ382_002779"
     CDS             476..1606
                     /locus_tag="JZ382_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAY2428905.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          495 a          280 c          359 g          588 t
ORIGIN      
        1 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
       61 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
      121 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
      181 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
      241 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      301 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      361 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      421 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
      481 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
      541 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
      601 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
      661 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
      721 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
      781 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
      841 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
      901 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
      961 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
     1021 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
     1081 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
     1141 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
     1201 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
     1261 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
     1321 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
     1381 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
     1441 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
     1501 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
     1561 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
     1621 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
     1681 agaaagagga attcgcaatg aaagtatctc aaaaaatcat at
//
LOCUS       DADJYG010000271         1707 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.280, whole genome shotgun sequence.
ACCESSION   DADJYG010000271 DADJYG010000000
VERSION     DADJYG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.280"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="JZ382_002780"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="JZ382_002780"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY2428906.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          520 a          354 c          365 g          468 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
     1681 tttattcact acctttattt tcctatt
//
LOCUS       DADJYG010000272         1706 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.281, whole genome shotgun sequence.
ACCESSION   DADJYG010000272 DADJYG010000000
VERSION     DADJYG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.281"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..408
                     /locus_tag="JZ382_002781"
     CDS             <1..408
                     /locus_tag="JZ382_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY2428907.1"
                     /translation="MLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIMLSITVVIRFF
                     PTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMTANAITKGIE
                     SPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
     gene            410..>1706
                     /locus_tag="JZ382_002782"
     CDS             410..>1706
                     /locus_tag="JZ382_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428908.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHD"
BASE COUNT          624 a          219 c          299 g          564 t
ORIGIN      
        1 atgttgccgg tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta
       61 ttatcctctt tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg
      121 atcagattct ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg
      181 agaaacattc gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc
      241 gttcttatac gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg
      301 atagaatctc ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat
      361 atcatcctgt tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt
      421 gtttgaattt aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga
      481 ccttaatttt tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg
      541 gaaatccaca ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt
      601 ggttggggaa attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc
      661 aaaaaatatt ggctacgtaa gtcaagatcc tagaggacaa ttctttacaa caaatacaac
      721 gagtgagtta gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag
      781 aatcaatgaa ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc
      841 gctatcaagt ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa
      901 attaattata ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc
      961 tgacttactt tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt
     1021 ttactatttg aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac
     1081 tacaactatt cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt
     1141 taatagttcg attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga
     1201 agaaattact tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt
     1261 tattggtttg gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa
     1321 gattattcgc cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt
     1381 tttagtaatg caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc
     1441 cctggggaat gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact
     1501 ttataaaatg aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt
     1561 ggtttctata gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg
     1621 gttggattat attaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg
     1681 tgcagtagtt atagctactc atgatc
//
LOCUS       DADJYG010000273         1663 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.282, whole genome shotgun sequence.
ACCESSION   DADJYG010000273 DADJYG010000000
VERSION     DADJYG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.282"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1412
                     /locus_tag="JZ382_002783"
     CDS             <1..1412
                     /locus_tag="JZ382_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAY2428909.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1663
                     /locus_tag="JZ382_002784"
     CDS             1546..>1663
                     /locus_tag="JZ382_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HAY2428910.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEIS"
BASE COUNT          659 a          251 c          356 g          397 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gtt
//
LOCUS       DADJYG010000274         1613 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.283, whole genome shotgun sequence.
ACCESSION   DADJYG010000274 DADJYG010000000
VERSION     DADJYG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.283"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            146..805
                     /locus_tag="JZ382_002785"
     CDS             146..805
                     /locus_tag="JZ382_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAY2428911.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            824..>1613
                     /locus_tag="JZ382_002786"
     CDS             824..>1613
                     /locus_tag="JZ382_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAY2428912.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEI"
BASE COUNT          628 a          192 c          308 g          485 t
ORIGIN      
        1 atagaggaag aaacattatt tcaatgatta agagtcgaga gcacaaacgg tagacgatgt
       61 tatttttata aaaagatata atttattaat ggtcgataat tatgaaagga aaaatattct
      121 taaaaaaaag agtataggat gtcgtatgaa aatagtcgct atagtaccaa tgaagttaaa
      181 taacaaaaga ttgccacaaa aaaacacgaa agcttttacc aatggaaaac cattgtgtta
      241 ttatatactt tcaacacttt taaaagtaaa acagattgat aaagtgtatg tctattgtag
      301 taacccagat attaaagaat ttataccaga aggtgtaaca tttttaaaga gatctagttc
      361 cttagatcag gatacaacga gtattacaca ggtattagcc ggtttcacaa aagaagtaga
      421 ggctgatgta tatgtaatga cacacacaac agcaccattt attagcgaaa aatctattga
      481 aaaaggacta gatgccgtgc tgacaggaga atatgattct gcatttgctg caaaaaaatt
      541 gcaagatttc ttatggaaag acggtgaacc atttaattat gaattggaca atattcctag
      601 aacacaggat ttgccgcctt tatacgaaga aactagtgga ttttatattt ataatcgcaa
      661 tgtgatgacc gagttgcata gaagaattgg gaaaaaacct tgcatagttg aagtaggtga
      721 gattgagtca gttgatattg atgaagccga ggattttatg attgctgatg cgatttataa
      781 tcatcttatt ttacaaggta gttaagacag ggggaaggtt aggatgaaca atgtacagat
      841 tttagattgc acacttagag atgggggcta ttgtaacaat tgggaatttg gatttgataa
      901 tacaaaaaaa attatttctg gacttataga atcaggaatt gagattatag aatgtgggtt
      961 tctcacaaat aaaacagtat acaataaaaa tgttacaaaa tatacgacct tagaagaatt
     1021 agcacagata attcctgaaa aaagagaagg aaaattattt gtagcaatga tgaactatgg
     1081 cgaatataat atagaggatc ttccagatta tgatggttca tctattgatg gaattaggat
     1141 tgcgtttcat aaaaaaaatt gcaatgaagc gttgaaatta tgtcgtgaga taaagaaaaa
     1201 aggctactta gtttttgttc aagctatggt atctctcaat tatacagatg aagagttttt
     1261 agcagtaata aaaaatgtga atcagtttaa accatacgct ttttatattg ttgatagttt
     1321 tggaatgatg aaaggaaaag atttaactcg tcttttttac atgattgagc ataatctaga
     1381 aaaagatata cagataggtt ttcactcaca taataacatg cagcttgcat attctaatgc
     1441 acaaaaactt ataaccgtac aaacaaatcg aagcctgatt attgattctt cggtgtacgg
     1501 aatgggaaga ggagcaggta atcttaacac agaactattt gtagaatatt tgaatgataa
     1561 tgcaggaaaa aaatatcaat taaaaccatt attatatata attgatgaaa tac
//
LOCUS       DADJYG010000275         1586 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.284, whole genome shotgun sequence.
ACCESSION   DADJYG010000275 DADJYG010000000
VERSION     DADJYG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.284"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            108..950
                     /locus_tag="JZ382_002787"
     CDS             108..950
                     /locus_tag="JZ382_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY2428913.1"
                     /translation="MNANSIVVGNFKGGTGKSTSTQMLGFVNAFYKKRKTLLIDLDPQ
                     ANASEVMSFTADNLGNHFYQENGFPKTIWNVLYDGSLDGAVLPLIENLDLIAGNIAMS
                     DYSDFMSAKFPNDKLAQFEYFANILAPLKEEYDEIFIDVPPSLGTIVQSAMYFAEYVA
                     IMLQTQPKSIRGAADYIDYMEFFTERYNTQLGIAGIIPFMLDTKTSTESYMYNEAKKL
                     YGDNLINTIVFRSARLIRYDETGITLETKKNGELKKVDANPQNTFISILDELNEHISW
                     FEEE"
     gene            953..1507
                     /locus_tag="JZ382_002788"
     CDS             953..1507
                     /locus_tag="JZ382_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428914.1"
                     /translation="MAERQSKLFPNKKARPRTNGMKDMEEFTKSLSISSADNSAPKVN
                     TTEKHSDDNKNLKNPNKSHKGKAGRKAEYKDPRLKKDLNTKISLSTKLRIQRLISRKF
                     DNKSEGDVIDIALDYLVSSFDRDDRDSLYKAYKEDMESMIPIIQEKNEKAKKQGKLTL
                     DITDEINQQTLKEQKEKWVSAKFE"
BASE COUNT          641 a          226 c          258 g          461 t
ORIGIN      
        1 cgtaatcaaa aaaataattt gattacgaga tacttattta catttacaac agcaagttgt
       61 atacttaatt tgaataaaag atacttaatc aaaaggagcg taataatatg aatgcaaatt
      121 caatagtagt tggtaatttt aaaggaggaa caggaaaatc tacttctact caaatgctag
      181 gctttgtaaa tgcattttac aaaaaaagaa aaacattgct tatagatctt gatccacaag
      241 caaatgcttc agaagttatg agtttcactg ctgacaactt agggaatcat ttttatcaag
      301 aaaatgggtt tcctaaaact atatggaatg ttctttacga cggtagttta gacggcgctg
      361 tccttcccct aattgaaaat ttagatttaa ttgccggtaa tattgctatg agtgactact
      421 ctgattttat gagcgctaaa tttccaaacg ataaattagc tcaatttgag tactttgcga
      481 acattctagc acctttaaaa gaagaatatg acgaaatttt cattgatgta ccaccttcac
      541 tcggaactat tgtacagagt gcaatgtact ttgctgaata tgtagctatt atgcttcaaa
      601 cacagccaaa atcaattaga ggggctgctg attatataga ttatatggaa tttttcactg
      661 aaagatataa tacacaacta ggtattgctg gaattattcc atttatgtta gacacaaaaa
      721 caagtacaga aagctatatg tacaatgaag ctaaaaagtt atatggcgat aaccttatca
      781 acacaatcgt tttcagaagt gctagattga ttagatacga tgaaactggt attacattag
      841 aaacaaagaa aaatggggag cttaaaaaag tagacgcaaa tccacagaat actttcatat
      901 ctattttaga cgaactaaat gaacatatta gttggtttga ggaggaataa agatggctga
      961 aagacaatca aaactatttc ctaacaagaa agcacgtcca agaacgaatg gtatgaaaga
     1021 tatggaagaa tttacaaaat ctttatctat atcttcagct gataattccg ctcctaaagt
     1081 taatacaaca gaaaaacatt ctgatgataa caagaatcta aaaaatccaa ataaaagtca
     1141 taaaggtaaa gctggcagaa aagctgaata caaagatcca agactaaaaa aggatttgaa
     1201 tacaaaaatc tctttaagta ccaaattaag aatccaacgc cttatttcaa ggaaatttga
     1261 taataaatct gaaggtgatg taattgatat tgctcttgat tatttggtat cttcatttga
     1321 tagagacgat agagattcat tgtataaagc atacaaggaa gacatggagt caatgatacc
     1381 aattattcaa gaaaaaaatg aaaaagctaa aaaacaaggt aaactaacac tagatatcac
     1441 cgatgaaata aatcaacaaa ctttaaaaga acaaaaagaa aaatgggtat cagctaagtt
     1501 tgaatagaag atacttaaaa caacataaac tattgattga agtatctggt aatattgatt
     1561 ttaagatacc tcaatcaata gttttt
//
LOCUS       DADJYG010000276         1577 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.285, whole genome shotgun sequence.
ACCESSION   DADJYG010000276 DADJYG010000000
VERSION     DADJYG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.285"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            62..718
                     /locus_tag="JZ382_002789"
     CDS             62..718
                     /locus_tag="JZ382_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY2428915.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            718..>1577
                     /locus_tag="JZ382_002790"
     CDS             718..>1577
                     /locus_tag="JZ382_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY2428916.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYP"
BASE COUNT          678 a          165 c          278 g          456 t
ORIGIN      
        1 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
       61 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
      121 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
      181 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
      241 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
      301 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
      361 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
      421 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
      481 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
      541 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
      601 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
      661 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
      721 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
      781 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
      841 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
      901 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
      961 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     1021 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     1081 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     1141 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     1201 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     1261 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     1321 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     1381 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     1441 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     1501 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     1561 agttcatcaa attaccc
//
LOCUS       DADJYG010000277         1576 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.286, whole genome shotgun sequence.
ACCESSION   DADJYG010000277 DADJYG010000000
VERSION     DADJYG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.286"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            83..319
                     /locus_tag="JZ382_002791"
     CDS             83..319
                     /locus_tag="JZ382_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428917.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            343..684
                     /locus_tag="JZ382_002792"
     CDS             343..684
                     /locus_tag="JZ382_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428918.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(677..856)
                     /locus_tag="JZ382_002793"
     CDS             complement(677..856)
                     /locus_tag="JZ382_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY2428919.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDMTK"
     gene            1078..>1576
                     /locus_tag="JZ382_002794"
     CDS             1078..>1576
                     /locus_tag="JZ382_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428920.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNS"
BASE COUNT          588 a          206 c          290 g          492 t
ORIGIN      
        1 gaaatattaa gaataaggat gtgaatttca actccttcta aattgttctt attatctatc
       61 atccgttgct gtctattaat ttatgtattg gagggtatat aaaatgaaga tcgcttccga
      121 aaaaattgat caactaacaa ttttagtaaa ccaaagaatc gaagatatta tcaaagaatt
      181 cgaaggtgaa aagattaaga acataaagct tactttaatg gatcataagt tcgaattaaa
      241 ttcgacaaaa aggaagatcg ttgagcttat agaagaagaa atagctctcc agatcaatga
      301 gcaacaatta gctaattaat ttatgtattg gaggggaaat gaatggaagt cgaacaaata
      361 aagtatcaag gtatggatcg ggaacggttg attgatatta ttgagcaaca acgtttagaa
      421 ttgatccaaa aagaagctgt atgtaaagag tacaaaaaac atttggaaca agtaattgag
      481 tatcattcag tagaaaagta cagatcagtg gtgcaaaaaa atagagcaac gagtcctgat
      541 tctcgtcaag atgtatcgat tagtatcaag acgccagtat ttaattctgt taaatcagaa
      601 aagctagaaa gtatctcaac tgctttggta gaaaaagcag ctgaaatgaa ctccctagca
      661 aatgaagtta gtcgtcttat ttagtcatat cgataacttg agagtctttg gtgattccgt
      721 tttttataga attaatggtt tgttcagcgg aatatttagt caagtatgtt tcgctagttg
      781 ctactacttc attgttattg gacttaataa cgaagtaata ttggccatta gttgcttctc
      841 ttataacaaa gtacaaattt ttcaccacct ataatttatt tcagcggacc actcgctgat
      901 aattaaaatt ataagctatg tataaaattt cacaatatca atttgtcgct gtggcggaaa
      961 gggtagacgc aataaaggat gaatgggaaa agtcaatgag taattatctg aaatttacaa
     1021 gtcccctgtt tagttatcta agattatttg ataatattat ttctgaggtg atagaaaatg
     1081 gtgggcgtag ttaaatttct tgggtttaca tttgatggat atggtacgtt aggcgaatgg
     1141 gtaggtatta ttttgatagt gattacaatt caacaaacat caaagtacta ctggtattct
     1201 aatagttcta aaattgtatc aagaataact gaaggaataa ccaaaaaaga tattactgtc
     1261 gtcggtaaat atgggtttac tataacttta ttaaatgaag ggaaatctga aaccgcaata
     1321 aagttttgcg ggattatagg taaatataac attttccaac gttttatcag aaagcattgg
     1381 tacaaatata tttacaaata ccctgtagtt aaaaagctta ttgaaaaata tgtggaccac
     1441 gaaatatctt atctcaatcc aacagacctt attaatcaag aattccagaa tattggatac
     1501 ttagaaaaag gtctaaaaat accaattgta aatgaaagtt tagttactgc aatggtagct
     1561 aacataaaga acagca
//
LOCUS       DADJYG010000278         1574 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.287, whole genome shotgun sequence.
ACCESSION   DADJYG010000278 DADJYG010000000
VERSION     DADJYG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.287"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            246..>1574
                     /locus_tag="JZ382_002795"
     CDS             246..>1574
                     /locus_tag="JZ382_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAY2428921.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSR"
BASE COUNT          571 a          219 c          281 g          503 t
ORIGIN      
        1 cttttttgtg gttgtttacg caaatggcgc aaatcttgta cattttaaca caataaaaca
       61 aaaaatatag aatatttcct cataaatttt tccatttttt gcaatattct caaaaaaaag
      121 ataaaaaatg agaataactg atgaaaaaac aagcataatc catcataaaa tcagtatagg
      181 tgaaaagatg aactttcatc tgcatatcaa gtaattggta tgaggcattt tttgaggtgt
      241 aggatatgaa gttggattac cttatagaag aggaagatag agaaaaactg ctgctagcta
      301 gatatttaga atttgagaaa aagaattatg tagtgctgcc taagttatat gaaacattag
      361 gtttgtctaa atttaaaaca agaaattata tcatagaatt aaataaagaa tttactgaat
      421 tcaaaacaaa gccagagatc caaatttcag aagaagcaga gatccatgtc gaacgtctga
      481 atttgacgga tatcaaaaat ttaagaagaa tctactttca acgatcaaaa attgctcgac
      541 tgttaacgga gatcgtggat tcaaaagtaa cgatcgaaaa atttgcagag aatcattttt
      601 taagcatatc aagagcctat gtaaaaagaa gagaattagt ctcttttctt gaacagcatc
      661 aaataaagtt aaagaaaaac atattagttg gggaagaaat aaacatacgg aacgtattat
      721 ttggggtata tttcggggtc tttaatggtt tttctatgcc atttagagaa agcgtcatga
      781 atgaagcaaa gagtatcgtg caatattttg aatatctgtt caatttaaaa ctgtctgcaa
      841 caaaacgaat caaattcaat ttgctaacca ctattagttt gttgagggcc tatgggaatc
      901 aaccaatcgg aaaacgtttc ttttcagatg aatactttaa gagctcagaa ggaatgaaga
      961 tcatcaagga tttatcacat atttcttttc ttcatatccc taaagagcag atcattgatg
     1021 agatttctta tatcatgttg tttttgatca cggaagaaga atttgaatta gatggcgcat
     1081 ttggacttgc ggaagctcat tttcatgata tccacatgat tagtcaaaaa atgtcagtgg
     1141 aattgattga tcaattacca ttttcgattg aaatctctga agaagaaaga ctatatatac
     1201 gaaaagaact tcgtatgggt atgatgcatg tcaatcgaaa aaattggata ttcgactttg
     1261 agatgactag ctttacgtct aaaaagcagc tacagttttt cttcgagacg tatccaatat
     1321 tcagtacagc aatccaaaag gtcatacaag agaataaacg ctatttcttg ggaaaagatg
     1381 aacgtttcat gattcgctta ttttatgatt atctcttttt attggtcggt atttgtccgg
     1441 tatcatattt tgaagcaccg attcatgtct gtattgattt ttcgcaagga tctgcttata
     1501 ccgattacat tatttctcaa gtgaaaggat ttaaaaatta caatattgtg attgagagtc
     1561 gaatcacttc tcgt
//
LOCUS       DADJYG010000279         1562 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.288, whole genome shotgun sequence.
ACCESSION   DADJYG010000279 DADJYG010000000
VERSION     DADJYG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.288"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..403
                     /locus_tag="JZ382_002796"
     CDS             29..403
                     /locus_tag="JZ382_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAY2428922.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(517..1146)
                     /locus_tag="JZ382_002797"
     CDS             complement(517..1146)
                     /locus_tag="JZ382_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAY2428923.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1280..1352)
                     /locus_tag="JZ382_002798"
     tRNA            complement(1280..1352)
                     /locus_tag="JZ382_002798"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1317..1319),aa:Thr,seq:ggt)
BASE COUNT          553 a          227 c          268 g          514 t
ORIGIN      
        1 taagaaaaag aaagagggcg tattagttat gtatcttaaa attacggaac aagcacaaga
       61 aagattacaa aaatatattg atgaaggcgc aacagttata ttggatttag atgatggcgt
      121 tggggaatat tctaaaatgg gtgtttgctc tttagatact agttttcgtt tacttcttct
      181 tgataaagaa cagagaaaaa atgattataa actccaatta gatagtgata ttggggatgt
      241 ttatataaaa gattattcaa aaatgtacat ggatgaacat atgacccttt ctttagatcc
      301 acgtctaggt gtatttactc ttgaaagccc aagtggttct ttagacagtc atgttcaatt
      361 agtagatcta cgaccagaaa aattctctgg aataaaacaa taactttctc tctattgtcc
      421 ataaaactag tctcaatcaa gctgtattga aaaaacaaga caacattctt accatggtaa
      481 ataaaaagac gacacatttt gtcgtctttt tttaaattaa ctattttcga taaaagatcg
      541 catagcttgt gccattattt ctggcttttc tctattcaat tcatgttttc cgtttttaat
      601 aaagagtagc cggccattcg ttgaaagttt atataaacgt tttgctgctg gtttattcgc
      661 atgatctttg gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa gctgtttttt
      721 gcagtctaat gtttcaagtg aacgaagtag ggtaagggtt tgttttttag atagacctaa
      781 tttttcaaat atcttagaag gtacgcagta aaaataaata ttctggaaag taagcaataa
      841 gcgattagga gactcaaatt gtggagcgac taaaacaacc ttttttaaat atggagaagg
      901 agttgcaaga tagtttaaag caataacagc gcctaaagat aatccacata aaataaaagg
      961 ttcctttatc tgattcaatt gtacttcgat gtatttggtt aagtcgtcta aagtagcagt
     1021 gtctgtgctg tttaaatgat tgaacaatga aagacttttg caagaataac tattgggtag
     1081 ataagagatt acatctttcc atgattgttc gtcttggcct agaccatgca aaaaaacaat
     1141 tttcatattg ctctcctttt tatagttatc atatagactt tctttgtact attaaaatat
     1201 aaacattcat ttcttacgtc ataaaataaa aaaacagcgc agacacattg ttaaaccaac
     1261 atgttgcact gtttaaggaa gccacttgcc gggcttgaac cggcgacctc ttccttacca
     1321 tggaagcgct ctaccagctg agctaaagcg gcattatcat taatacaaat aataggataa
     1381 cgaatattaa tgtatttgtc aacagaattt tttcgaaaaa atgtgtttct ctaataggaa
     1441 aacagatttt agaaaaaata aaaataacaa aaaatgcgaa cggtttcttt gataatataa
     1501 taaaaaaatt caaaaaaagt attgcgttat ttagaagaaa ttgatattat attcatgtac
     1561 tt
//
LOCUS       DADJYG010000280         1534 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.289, whole genome shotgun sequence.
ACCESSION   DADJYG010000280 DADJYG010000000
VERSION     DADJYG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.289"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..1490
                     /locus_tag="JZ382_002799"
     CDS             24..1490
                     /locus_tag="JZ382_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAY2428924.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          498 t
ORIGIN      
        1 tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc
       61 cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta
      121 tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga cttggattac
      181 cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta
      241 tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg
      301 gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt
      361 ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca
      421 tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt
      481 acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag
      541 aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac
      601 aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt
      661 tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg
      721 ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata
      781 caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa
      841 aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag
      901 caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg
      961 aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga
     1021 atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg
     1081 aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt
     1141 acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt
     1201 ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt
     1261 tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt
     1321 tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg
     1381 gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc
     1441 tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca
     1501 taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DADJYG010000281         1529 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.290, whole genome shotgun sequence.
ACCESSION   DADJYG010000281 DADJYG010000000
VERSION     DADJYG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.290"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(99..1229)
                     /locus_tag="JZ382_002800"
     CDS             complement(99..1229)
                     /locus_tag="JZ382_002800"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY2428925.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT          434 a          309 c          240 g          546 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatt
//
LOCUS       DADJYG010000282         1508 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.291, whole genome shotgun sequence.
ACCESSION   DADJYG010000282 DADJYG010000000
VERSION     DADJYG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.291"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            100..867
                     /locus_tag="JZ382_002801"
     CDS             100..867
                     /locus_tag="JZ382_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY2428926.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1059..1457
                     /locus_tag="JZ382_002802"
     CDS             1059..1457
                     /locus_tag="JZ382_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAY2428927.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          560 a          189 c          282 g          477 t
ORIGIN      
        1 atactgttaa atatctcgaa aaatcaatta cgagttattt gccttatgtg aacctttatg
       61 attaacaact gaaaaaatgc gtgaaggaag gaaaaacttt tgaaaaaaga tgaactgaca
      121 aagtacaatg aggagttctt agcaacaatt gacatgaaaa tggatttaaa gtcgtatgaa
      181 ctttatcaat tgctgttcag ttatatctca cccacaaagc taaagaaaac aggcgtagta
      241 gatttacctg ttaaagcgat tattaattac atggatgttg atcaaaatct tgaaacggaa
      301 ctaaaaacca tagttaacac tttgcaaaga caagcagttt atgaaatttc gaatctagac
      361 aaaaaatata agacgtatac tactgtatct gctattgatc ggattcattg ggatttagaa
      421 gagcatagtg tatctttcag tttttcagaa gatgtaattc ccttaattgt ctcaatcaaa
      481 aagaagtatc cgttctttgt ttttgaagac ttagcatctt ttcaaaaaaa atatagcatc
      541 gtgttataca aatatttggc gatgtattat aaaaaatatc ttatcgccaa aaataaagga
      601 gattttgaga aagctgaaat atataagaat cccctgattg aaatagttaa aattcgtgag
      661 attacgaaaa cagaaaatga gtataaaaca atatcgaatt ttgatagacg tgttttaagg
      721 gaatcagttg aggaaattaa tgaaaaatca atgctgaata ttgattttgc taaagttaga
      781 aaaggcagaa attacaattt tgctgtccag tttaatattt ctagtgagtt tgatgaaaaa
      841 acttatagag aaaaaaaatt aggttaatgc aaaatatatc ttactcgttt gaagatgctt
      901 cagacgtatg ctaagatata tagtgccttg ggagcaaggt attaatctcc tttagaggtt
      961 aattagcttg tgatcgccta tcaatttttt tggtaggcga ttcttttttt tgaaacaaaa
     1021 aagcaaaaat tattttttaa ttagagaggt gcaatgcgat gaaatacttt ttttgtgcag
     1081 tattcgaatg gaaaggaaat gaatatgcag ttaaattccc agattttcca gacttatata
     1141 cacatggtga aactttagaa gatgcttttg agatggcgca agaagtattg gaaggatact
     1201 tggtgctttt agagttgcaa gaaaaagaaa ttccatcgcc tacgcctgtt caagagttaa
     1261 atgcaagaat caagcgatct gagaatgtta atttatttgt cgtaaaagct aatacgaaag
     1321 ttgctaaagc tcgaaacaag caaaattatg atcgaaaatc agtgactgtg gaacactatt
     1381 taaatgtttt agcggctgaa gagaatatta atctgtcgaa agctttgaat gatttgttga
     1441 gagaaaagtt gatttgatgt ctctcagatg atcatattaa gcaaaaacag cttgttttag
     1501 gacgtatg
//
LOCUS       DADJYG010000283         1497 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.293, whole genome shotgun sequence.
ACCESSION   DADJYG010000283 DADJYG010000000
VERSION     DADJYG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.293"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..359
                     /locus_tag="JZ382_002803"
     CDS             <1..359
                     /locus_tag="JZ382_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428928.1"
                     /translation="IPTFADSTNEPTTVVDNNQELTPLDRDGIAIGNPTNDLTVTENY
                     LIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKS
                     AGNNWIATYSGTLTRK"
     gene            369..572
                     /locus_tag="JZ382_002804"
     CDS             369..572
                     /locus_tag="JZ382_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428929.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            783..1367
                     /locus_tag="JZ382_002805"
     CDS             783..1367
                     /locus_tag="JZ382_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428930.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          528 a          219 c          278 g          472 t
ORIGIN      
        1 caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat aataaccaag
       61 aattaactcc tttagatagg gacggaatag ccattggtaa cccaactaac gacttaactg
      121 ttactgaaaa ttatttaata gtttctccat tacttgctct agcagcttct aaaacgatac
      181 gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac
      241 ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt
      301 ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg
      361 gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa
      421 gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga
      481 cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa
      541 gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga
      601 cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac actttctttt
      661 tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta
      721 tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt
      781 ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag
      841 acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa
      901 gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg
      961 tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac
     1021 tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa
     1081 gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt
     1141 tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg
     1201 gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg
     1261 gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt
     1321 atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta
     1381 atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa ttttttgtaa
     1441 gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg ttaatat
//
LOCUS       DADJYG010000284         1495 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.294, whole genome shotgun sequence.
ACCESSION   DADJYG010000284 DADJYG010000000
VERSION     DADJYG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.294"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(332..1465)
                     /locus_tag="JZ382_002806"
     CDS             complement(332..1465)
                     /locus_tag="JZ382_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2428931.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT          461 a          300 c          243 g          491 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaagagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acatttttga
      121 tcctttttat ttgattaatt atatccagct ggataataat tttgggggca acatttctat
      181 cttactatta tcttcctaaa gtagtagact ttcttattgg gaaaaattac aacaaaaagg
      241 agtggcttag ttgacaactc ctttttgttt acaaatcaat tggatatcta taaattgtat
      301 tttctaacga attttataga catctgattt gtcaaattaa gctagacaat atgtctttat
      361 ctatgcagct caaaaatact tccaatggtg ttcgataatt taatgatttt cttggaatat
      421 ggtttctttt cgaggcaacg gattgaataa aggtttcatc cgtgtcccta aagtccattg
      481 ttttaggtaa gccatcctgc ttgagcccat gcactcggta ctgatgagaa aaaattgatg
      541 cctgtttctc ctgaggaatg ccaattccct gatcttttac ttcaataatc acgctaccct
      601 tttggtttga aatggtgaca attaccagtt ttccttcacc atatttgatt gcattgtcaa
      661 ctaaattcga aaccgcccga taaatccaac gatctcgtcc atgaatcagg gttggttgat
      721 cttcagtaaa cgcaaacaaa agattcggat aattcatttt gtaggcatca caaacttccg
      781 cacagatcag tgaaacgtct acgacttccg tgctaagatc ctctgagtta ttggataatg
      841 tcaaaatatc gtcaatactg tcggtcatcc gatctaaaag cattaaattt ccttgatcgg
      901 catgttgccg ttctatttcc agattcgtac gcaaaatagc gatggcattt ttctgctcgt
      961 gggtcagata actatttaat cgtcgataat cctccaaatg accatcgaac ttatccttta
     1021 gcttattaaa agccgcattt aatgcctcgt ccttcacagt atttttagga acttgatcaa
     1081 taaacgttaa atcacgcgta atcgctaaga tttgattact gtaaattttg cgtaaaacca
     1141 accaaagaat aaacgttaat aacatgacgg cagaacaaat aaccagcacc atcgctggta
     1201 aatatttaaa tagttcgtcc tggtaagctt tatctatcat cgaaatagtc ataaattgat
     1261 tgttggaaga tttcgaagct tccatctgcg tctctgcttg aaccgtctta ccctcttcca
     1321 taaaataaac atcttcacct aaggtagatg cgatactgat tccctccaaa ttatttctca
     1381 gaaagaagaa actcactaaa atcacgcctg tcaccaacag tgaaaaagcc agcatcgtta
     1441 acgtcatttt aaaatttaat agcatagctg gtaccccttt gctcgaatgg ttttt
//
LOCUS       DADJYG010000285         1477 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.295, whole genome shotgun sequence.
ACCESSION   DADJYG010000285 DADJYG010000000
VERSION     DADJYG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.295"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(138..428)
                     /locus_tag="JZ382_002807"
     CDS             complement(138..428)
                     /locus_tag="JZ382_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428932.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="JZ382_002808"
     CDS             complement(546..806)
                     /locus_tag="JZ382_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428933.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="JZ382_002809"
     CDS             complement(943..1356)
                     /locus_tag="JZ382_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAY2428934.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          543 a          242 c          218 g          474 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaag
//
LOCUS       DADJYG010000286         1470 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.296, whole genome shotgun sequence.
ACCESSION   DADJYG010000286 DADJYG010000000
VERSION     DADJYG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.296"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(141..1433)
                     /locus_tag="JZ382_002810"
     CDS             complement(141..1433)
                     /locus_tag="JZ382_002810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY2428935.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          400 a          329 c          264 g          477 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca
//
LOCUS       DADJYG010000287         1456 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.297, whole genome shotgun sequence.
ACCESSION   DADJYG010000287 DADJYG010000000
VERSION     DADJYG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.297"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            34..546
                     /locus_tag="JZ382_002811"
     CDS             34..546
                     /locus_tag="JZ382_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428936.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            817..1440
                     /locus_tag="JZ382_002812"
     CDS             817..1440
                     /locus_tag="JZ382_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY2428937.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          571 a          178 c          250 g          457 t
ORIGIN      
        1 atgataagta aagttctaag tttgtgatga taaatgaaat taaatagtat aaatgattta
       61 tttgctatta taggtgttat tgctaacata cttttttcgt gtttgaattt tagatttact
      121 caaaagaaca aaaagaacaa actcagtata gaacaaatga aaaaagacat tgaaactgat
      181 ttaaaaaatg accataaaga aagaagaaaa attgaagaat atacttcttt aatggaaaag
      241 tgtaaagcaa ctgatgcaat ttttagcttt ttaagaaaaa gtgatgagat aatcacacgt
      301 gaaaatatag atttagaaaa tgagattagt aaacttgaat ctatcttttc cgatataaat
      361 tctcttaagt atttaatcgt cgaagaaaat tcggatagct ttttagaatt tgaaaaatat
      421 ctaacagatc ttatagataa tagaaataat gaattaatga gacaaaacat tgcaagattc
      481 cttactaaat tagtttctga atggaaaagt attatttctc aaaatatgga taaattaaga
      541 tattaaggat gttagtttta aaataaacta atgtccttat ttttgtgttc acatttctgt
      601 tacctaagaa atacttaaag gaggtgatgt agccagcaac gactacaact tgaacccgct
      661 atctacagtt tatttgttgt ttcttttggg tatactctaa tggtacaaat ccatatttgc
      721 ttaaagcgtt gatttttcag cggtttttct ttattgcaaa gtgtacccta agacctgtat
      781 actaatagta aatagattgg ataggaatga tggcaaatgt atttagctta tttaagagtt
      841 tcttccgacg atcaaagttt agctcggcaa cataagctaa ttgaaaattg gtcagtcaaa
      901 aataatattg ctgaagacga tttaaaaatt ttcgaggaaa aagtctctgg aaaaaatatt
      961 gaaaacagac cagagctgca agagcttatt tctttcatac gtgaacgaga tacgattatt
     1021 attcctagtc ttgatcgatt agcaagaaat agtaaagata caaaagactt acttcaattg
     1081 tttcgtagta aaggtgcttc tattgaaata ttagacctac cgtcgtttaa tggggtaact
     1141 gaccctgcat taagagactt gctaacaaat ttagtcatag aagtgtttag ctatgttgct
     1201 gaaaacgaac ggaaaaaaat caaagagcgt caaaaacaag gcattgaaat tgcaaagaaa
     1261 aatggtcgtt atactggtaa aaagattcaa taccatgcca atgcaacagg aaaaaacaaa
     1321 ttgatttacg atgaaattgt tgatggtctg aagaaaaaag aaactatttc taaaatagca
     1381 aagaacgcag gagttacaag aaaaaccgtt tatcgagtaa aaagagattt aggaatgtaa
     1441 tatgttccag ttagaa
//
LOCUS       DADJYG010000288         1452 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.298, whole genome shotgun sequence.
ACCESSION   DADJYG010000288 DADJYG010000000
VERSION     DADJYG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.298"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..625)
                     /locus_tag="JZ382_002813"
     CDS             complement(<1..625)
                     /locus_tag="JZ382_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAY2428938.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="JZ382_002814"
     CDS             complement(784..1050)
                     /locus_tag="JZ382_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2428939.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="JZ382_002815"
     CDS             complement(1040..1396)
                     /locus_tag="JZ382_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY2428940.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aa
//
LOCUS       DADJYG010000289         1440 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.299, whole genome shotgun sequence.
ACCESSION   DADJYG010000289 DADJYG010000000
VERSION     DADJYG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.299"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            105..1406
                     /locus_tag="JZ382_002816"
     CDS             105..1406
                     /locus_tag="JZ382_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAY2428941.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DADJYG010000290         1438 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.300, whole genome shotgun sequence.
ACCESSION   DADJYG010000290 DADJYG010000000
VERSION     DADJYG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.300"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(337..951)
                     /locus_tag="JZ382_002817"
     CDS             complement(337..951)
                     /locus_tag="JZ382_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAY2428942.1"
                     /translation="MSLCYFDYYYQLEEKLRIEEPLMVQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDELSVKPEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQETRGK"
BASE COUNT          470 a          285 c          237 g          446 t
ORIGIN      
        1 gtaaagagtt ttggggaaga ctctttcatc aacgtgtatt aagagacaag aaagaatccg
       61 aatggattct ttcttgtctc tttttccgct atttttttgt ctatctagca ttacatattt
      121 ttgtagctct ttttttctca ttgtgaaaca acttataaaa ataacacact ttcgaaaaaa
      181 aaggctgacc tctggagcta aacaaactta aaataacaaa aaaataaaaa atgatcatta
      241 ctgctcttct ttaaaaatcg ttttctttgt ttcaaacact ccatatttac gtggaacacc
      301 atttttttaa agaaaatttg gtcattatgt ggaaaactat tttcctcttg tttcctgaac
      361 tagctgtttt gctgtaaaat acaaaacttg ttgaagcaaa aagatcgttg caagagaggc
      421 tgttccacag attgctggat ctaaattcgg caaagtcaag actgcatcat gcagattggc
      481 atggttatcc aaaactaaat cagcaaattc aaataatctc aaaccactgc tatgctggga
      541 cttgatgctt cgtgacaaat caaacccagt aacaatgatc aaaggatgtc cattttcttt
      601 gatccattga gccaactcaa tgattgctgg atttcttcct tcattcgaaa gaagaagaaa
      661 aatgtcttct ggtttgacag aaagctcatc aataatcgct tgtcctgccc cttctagcgt
      721 ttcatagaca cctccggctg ggttctcaat caatatactt ggcacaaggt ccggtgtttg
      781 atcccaaaat tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctgcc
      841 gccttgattg atttgtttcg caatccaagt tcctgcttga accatcaacg gctcttctat
      901 ccttaatttc tcttccaatt ggtaataata gtcaaaataa cataaactca tttttctctc
      961 ccgcctagct atacttgaca tgaatagaaa cagctgccta atctaaggaa ctacataaaa
     1021 aacagaagat aaacagtaac acatgcggtc tttgctgttt caaacgcaaa aaaggtattg
     1081 aacgaatacg cccaatacct tgacatagca aataaaacaa cgtgcagacc atagaaaatc
     1141 tctacatagg ggtagaattt tccccttaga ttaggtacaa taaataaggt cgatgcactg
     1201 cattgactat ggcaaaatga tcggtcaggg tgtatgagtg tgactggtca ttccctgatt
     1261 cttagcgcca actaagggtc agggagcctt tttttattca gctgtttcta tctgaactca
     1321 ttgtaagtaa tctcttattc aagtgcaagt acgaaaatac aaaaaaacat tggtatgatt
     1381 tttttacaaa taaataacat aaaaaaaagg aatgacttgt ccctatgtag caagcatt
//
LOCUS       DADJYG010000291         1437 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.301, whole genome shotgun sequence.
ACCESSION   DADJYG010000291 DADJYG010000000
VERSION     DADJYG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.301"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            39..1208
                     /locus_tag="JZ382_002818"
     CDS             39..1208
                     /locus_tag="JZ382_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAY2428943.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          516 a          262 c          233 g          426 t
ORIGIN      
        1 tctttctgta aaaaagaaag gagactaaat aaactattat gaaaaaagga aaacatgcta
       61 aaaaaagaaa atattcattt ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct
      121 ctacaggaac tattcttgga tggcaatttt acaataatga acactctcag atagttgccc
      181 atgctcaatc agctcaaaaa aagttgatac tgactaccaa tgaacaacag ctcgttgaaa
      241 aaatcgatca actattaaga aaaaatgatt atattggctc aatttacgta aagaaaaata
      301 atcgcatcat catggaaaaa ggctatggct atgcgaataa actcacgcaa caaccaaatg
      361 atccttctct ttattatcag attggttcgg ttcaaaaagc attgactact cttttgattt
      421 taaaacaagt cgagcgtggg cacctttctt tagatactaa gctggctcaa ttctatccac
      481 aaattgctgg cagccagcaa attactatcc gtgacttgct gtatatgcgc tcaggcttgc
      541 accgaactgc ttctccaacc ataccgatga ctgatgaaga agtcgttcaa tttgcgattc
      601 agcacttaaa atttacagat tatcagacct ctcattatga ttcattgaac tttaccatat
      661 tgactggaat tttgatcaaa ttaacaaatc aaccgtatga aaaattattg accgatgata
      721 tcatcgaacc tttaggatta cagcatacaa aattttatga ccaagtcaaa aattcatcca
      781 atcatgcact atcctatcaa atgtctaaag aggatgatta ctttaaacca ctgacagaat
      841 ctgaaacaga gatccataac gaattaggga ctggtaatat cagtatgtcc gtctatgatt
      901 taaatacgct gtttacacaa gttttatctg gaaaactagt ttccaaagac ttactcttct
      961 cattatggcg tgaaaatgcg aatggacatc catatagtgg tggggtatat agcggagata
     1021 attatatcct gtcgcaagga aatatcgact cttttgatac atctgctata tttagcaaag
     1081 attcacagga tgctatcatc atggaaagca atataaaagc tgacaaaact atcaaactgc
     1141 ctactgctga tctaagaaac caaatctatg acctgatgga aggaactact aagttggcaa
     1201 atagctgata tgatgaaaag atagcaaata ctacttcttt gaatagcatt tgcttaaaaa
     1261 aagtactcga ctaaacgtag gaaagagtgt gggacaaatc taaaaatcaa tttttgctac
     1321 agactttaat tccaaataaa cagaacagaa agaggtgttc ttctttctgt tctgtccaaa
     1381 ttattctaag atttctcttt accaacatta tttgacaaag ggcctattaa atctaaa
//
LOCUS       DADJYG010000292         1428 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.302, whole genome shotgun sequence.
ACCESSION   DADJYG010000292 DADJYG010000000
VERSION     DADJYG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.302"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            282..1322
                     /locus_tag="JZ382_002819"
     CDS             282..1322
                     /locus_tag="JZ382_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY2428944.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT          489 a          159 c          329 g          451 t
ORIGIN      
        1 aaccagttta ggggtatata ttttacatga aaatcattat ttcattgatg gtatgaagtc
       61 tagaggctta atatgggatg ggatatttgg attaggcaaa aggtttttag atagggattt
      121 ttggatttgg tttataattg cagttgtagt tgtgtgcata atttgcatgg ctatcgatgt
      181 tgtaagagaa aaatttgaaa gacagatttt taaaattaag agtgtaaaga acctgtgtaa
      241 acttatgaat catgttataa atatataccc ataattaata aatgaggaga aaagaagtga
      301 aagttatatc attcggagaa ttattattga gacttgcagc acccggttat tcaaagttat
      361 ttcaaagaga tagtttggag actacattct gcggagggga agctaatgta gcggtatcct
      421 tagcaatatt tggatatcaa tcagaattta taacaaagct tccggattca gatgttggat
      481 atgcggcagc taatgctttg cgttattttg gagtaaatgt tgataatatt atatttggag
      541 agggacgcat gggactgtat tatttagaaa aaggagcagc tcagagacca tctaaagtaa
      601 tttatgacag aaaattttct gcaatttcgt tagctgatag tgatgaattt aagtggaagg
      661 agatcctaaa cgatgcaaat tggtttcatt ggacagggat aaatccagca ttgtctgaga
      721 atctctttga gacgtcctta gatgcatgtc aggctgccaa aaaaagaggt ataacaattt
      781 cttgtgattt aaattatcga tccaagcttt ggacatcaga ggaagcaaaa gaaaagatgg
      841 aacagatact accattggtt gatatttgta tttgtaatga agaagatgca gaaaaagtgc
      901 taggaattaa agctgagcat acaaatatta atagtggaga attaaataaa gatggctatg
      961 tgcaggtggc tagggagatt cacaagaagt ttggatgtag gtatgtagct acaactctgc
     1021 gtagaagtta ttcggcaagt agaaatggtt ggagtgcaat gttatacgaa gcagaaaaag
     1081 ataaagcgta tttttccaca gaatacaaca tacaaattgt tgatcgcgtt ggtggtggag
     1141 acagcttttc tgctggtata atatatgcgt tatgtgaaaa tatgtccgga caaaaggcta
     1201 ttgaatttgc gactgcatct agttgtttaa aacatactat agaaggtgat tttaatcgaa
     1261 ctacaattga ggatgtatat aatttaatga atagcggtgg aaatggtcgc gtgttgcgtt
     1321 agtcaacata taaagataaa tcacagtaat ttttatggtg attatctgtc ttagtagtga
     1381 ttagaataac gtgaaaataa attgttgagc gtttatactg ttttgaaa
//
LOCUS       DADJYG010000293         1417 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.303, whole genome shotgun sequence.
ACCESSION   DADJYG010000293 DADJYG010000000
VERSION     DADJYG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.303"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(150..1337)
                     /locus_tag="JZ382_002820"
     CDS             complement(150..1337)
                     /locus_tag="JZ382_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAY2428945.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DADJYG010000294         1413 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.304, whole genome shotgun sequence.
ACCESSION   DADJYG010000294 DADJYG010000000
VERSION     DADJYG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.304"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            104..1273
                     /locus_tag="JZ382_002821"
     CDS             104..1273
                     /locus_tag="JZ382_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428946.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          515 a          207 c          229 g          462 t
ORIGIN      
        1 cgtaaaatat tttgtgtaaa tagatattta gggtagaaaa aaagaaagtc cattctgtaa
       61 aattaaagtt accacaaccg aaattttata ggaggacttt ctcatgccca attttactac
      121 agaaattatg gaaacactaa tcaataaagg tgatttagat gaattatttc gatgtcattt
      181 agaattagct gttaattcat tattgcaggc tgaattaaca gcttttcttg attatgaaaa
      241 atatgatcgt gctgggttca attcaggtaa ttcccgtaat gggaattatt cacgttcatt
      301 cagaacagag tatggagaat taaatttggt aattcctaga gatagaaatg gtaagttttc
      361 tcaacaaact ttacctgctt ataaaagaac caatgactct ttagaaacca ctattattca
      421 gctatttcaa aaaggaatta ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg
      481 gcatcactat acgccacaaa ccatttcaaa tatgactaaa atagtttctg aagatattat
      541 tgcctttaaa gaaaggtcct tagaatccag atactctgtc atttttatgg atgctactca
      601 cattccttta aaaagacaaa ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg
      661 attagatgga accaaagagg ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt
      721 ttggaaagag attcttcaag acctaaaaga ccgtggttta aaagaggttt tattagtcgt
      781 aactgatggt ttaagtggca ttaacgatag tattcatagt gtttatccaa atgctcaatt
      841 tcaacaatgt tgtgtccata tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg
      901 acaagaaatc tgtagcgatt tcaaattagt ttatcaagca agttcaaaag aagaagcaaa
      961 taatcaaata aggtttatga tagataaatg gaaaaagcag tatccacgag tggttaaatt
     1021 gctcatgaat cctgctatat taacctttta taatttccct ccgtcaatca gaagaactat
     1081 ttattcaact aatttgattg aaggttttaa caaacaatta aagaaatata ccaaaagaaa
     1141 agagcaattt cctaatgaag aatctctgga gagatttcta gtatctcaat tcaacaacta
     1201 taatcaaaag tttctatgcc gagttcataa aggttttaaa gaaatacatg atacattaga
     1261 atcaatgttt taacttgatc aacagaatgg gcttttccat ttacacaaaa ttattgacgc
     1321 tcccgacaaa attatgatct tgcgtaaagt tataaaatat taataaaaat attttattaa
     1381 tgaaaagctt ggtagcacgg ttattcatat gct
//
LOCUS       DADJYG010000295         1397 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.305, whole genome shotgun sequence.
ACCESSION   DADJYG010000295 DADJYG010000000
VERSION     DADJYG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.305"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(252..1277)
                     /locus_tag="JZ382_002822"
     CDS             complement(252..1277)
                     /locus_tag="JZ382_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY2428947.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          416 a          231 c          275 g          475 t
ORIGIN      
        1 aataagggta aatgtgttta aaaaaacgaa ctataaaaac ttatacacaa cgtggttaaa
       61 aatagttttt tcttccctgt ggaaaaaata gtttaacaca aacattatag ttatccacag
      121 ctatctaaaa cagatggaaa ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag
      181 ggatgagtga ttgaaaaaag aacagagtaa ctgacttatc ctcagtgttt ctgttctttt
      241 ataattctgt tctacatttc ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa
      301 agaatgattc ttttttcttc agggtattgg tggtggatta gttggaataa aagttctgtg
      361 gctttgattc ctaactgatt tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg
      421 gaggacaata agggatcact gaatcctgcg atcgaaagat catcaggtat ctgctttcca
      481 gattgttctg ccactttgta aatactcaat gattttaatg catcggtaga aaaaatcgca
      541 gttggtcttt ctcgtagctt cattaaatca ttcattgctg ccatgctatc ttctaagcga
      601 aagccgccgt ctataatcca ttcattacgg atatctaggt ggtgatcaaa taagcattga
      661 cggtaaccat tgagacgatc attggataca taaacttctg tcggagcatg gagacagccg
      721 atatgacggt gtccattctt gatcagtgtt tccaccaaat cgtaactatc ctggaaatta
      781 tccgtgtcca ctgagtagat gttcgtgtag tcgtttccga cttttccgat taccacaata
      841 ggaatattat attgatccaa ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata
      901 ataattcctc ggatcatttt ttcttggatc tttttcgcgc atttttctag ctcttcttct
      961 ggtgtttttg ccgtttgtag aatcaagtcg aagttatttt gttcagaaac ggcagaaatg
     1021 gattgaataa catctgaaaa aaaaggattg ccataggtcg tatgagaagt acgatgagat
     1081 aatgctagaa tcgcgtcaaa accatttcct gacagtcctc ttgcaatttt gctcggttca
     1141 taattcaatt ctttgattgc ttgaagtaca cttgcttttg ctttatcaga gatatttggc
     1201 tggttattta atactctgga gacagttgat tttgagacat tagctttttt cgcaatgtca
     1261 taaatagtag gtttcaattt tatatgcacc tcctttttgg gagcgtttta gaataattcc
     1321 atagtacaat agacattctt ttttgtctat gattttgaaa atttaggaat agcttgcatg
     1381 atcatgagag accatat
//
LOCUS       DADJYG010000296         1385 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.306, whole genome shotgun sequence.
ACCESSION   DADJYG010000296 DADJYG010000000
VERSION     DADJYG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.306"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(737..1078)
                     /locus_tag="JZ382_002823"
     CDS             complement(737..1078)
                     /locus_tag="JZ382_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY2428948.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1078..1308)
                     /locus_tag="JZ382_002824"
     CDS             complement(1078..1308)
                     /locus_tag="JZ382_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY2428949.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          431 a          234 c          200 g          520 t
ORIGIN      
        1 tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa gtaattttat
       61 taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta cttttacatt
      121 cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg cacattttcg
      181 atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt agatttttca
      241 gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct tttcattgtt
      301 tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa attctttcct
      361 agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta aaatgacatt
      421 actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt ctttttcttc
      481 tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa
      541 gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat
      601 gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac tagtagaatg
      661 aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa aatattatag
      721 agtttactta caatgcttaa aacatatact gtataatctg atcaatttta gccatgtctt
      781 gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga gattttaatt
      841 gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa cgtgtcggta
      901 aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta cggttaaatt
      961 cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct acggagggat
     1021 caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct tctgacacta
     1081 taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat aatactcggc
     1141 ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg ttccatcatc
     1201 agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat cagaaggtaa
     1261 agttaccact acagaacttc cttgtaatct agattttgta acaagcatta tcatcactcc
     1321 cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg taaactatct
     1381 agaaa
//
LOCUS       DADJYG010000297         1373 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.307, whole genome shotgun sequence.
ACCESSION   DADJYG010000297 DADJYG010000000
VERSION     DADJYG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.307"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            191..445
                     /locus_tag="JZ382_002825"
     CDS             191..445
                     /locus_tag="JZ382_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY2428950.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="JZ382_002826"
     CDS             446..763
                     /locus_tag="JZ382_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY2428951.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DADJYG010000298         1372 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.308, whole genome shotgun sequence.
ACCESSION   DADJYG010000298 DADJYG010000000
VERSION     DADJYG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.308"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            122..>1372
                     /locus_tag="JZ382_002827"
     CDS             122..>1372
                     /locus_tag="JZ382_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAY2428952.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DADJYG010000299         1352 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.309, whole genome shotgun sequence.
ACCESSION   DADJYG010000299 DADJYG010000000
VERSION     DADJYG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.309"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            82..>1352
                     /locus_tag="JZ382_002828"
     CDS             82..>1352
                     /locus_tag="JZ382_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAY2428953.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          457 a          253 c          259 g          383 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg at
//
LOCUS       DADJYG010000300         1341 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.311, whole genome shotgun sequence.
ACCESSION   DADJYG010000300 DADJYG010000000
VERSION     DADJYG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.311"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            79..951
                     /locus_tag="JZ382_002829"
     CDS             79..951
                     /locus_tag="JZ382_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY2428954.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          413 a          255 c          304 g          369 t
ORIGIN      
        1 taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa
       61 ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg
      121 tgtgccattg gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca
      181 cctgaggaaa caatgccttt agtcattgac ttcttcaaac aataccaagc agatttagca
      241 gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg
      301 tacattacct cgaccccgaa attggcatgg caaaattttg atttcatcgg taccatgaaa
      361 aaagaatttc cgatcccgat ttcttggaca acagatgtca atgcggctgc ctatggtgag
      421 tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt actatacgat cggtacgggt
      481 gttggcggcg gtgccttaca agaagggcgc tttatcgaag ggtttagtca tccagaaatg
      541 gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg tccatttcat
      601 ggcaactgtt tagaagggat ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa
      661 ggacaagagg ttgcagaaga tgatccttat tgggaaatcg aagcctccta tattgctcaa
      721 tgtgcctaca atacaacctt gatgttttct ccagatgtga tcatttttgg cggtggtgtg
      781 atgaaacaac gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg
      841 tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc cagcattaga agacaacgct
      901 ggaacgctcg gttgtttggc attggctcga gaagcggtct tacattcata aacgaaaaaa
      961 ggctcttcga catttagtgt tggtagagaa agatacaact gaaaatttag tcataaccag
     1021 ttaggcagag tgcttaactg gtttttgttt tacttggaag atcaagcctt gaatgagata
     1081 aattcttgct cgaaagttat ggaaatttcg atagccgtag gccacttgta ttgtgcaata
     1141 gttttttaca cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca
     1201 ctcaaatcgt aatacccgct aaagcataca aggagagtga acataaatga cttataccca
     1261 tcttacacca aacgagcttg taatgataga agcatatttt catcaagaaa ctccggttgc
     1321 tatcgttgcg aagcagctta a
//
LOCUS       DADJYG010000301         1336 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.312, whole genome shotgun sequence.
ACCESSION   DADJYG010000301 DADJYG010000000
VERSION     DADJYG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.312"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..412)
                     /locus_tag="JZ382_002830"
     CDS             complement(<1..412)
                     /locus_tag="JZ382_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAY2428955.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            491..922
                     /locus_tag="JZ382_002831"
     CDS             491..922
                     /locus_tag="JZ382_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAY2428956.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(1021..1254)
                     /locus_tag="JZ382_002832"
     CDS             complement(1021..1254)
                     /locus_tag="JZ382_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAY2428957.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT          397 a          233 c          238 g          468 t
ORIGIN      
        1 aaagagtctt tttttctttt aattgtttat ggagagcctg acgtgcaaga ttatctgctc
       61 ctttattttt gttttcgggc aaccattgca ggatcaataa agaaaattcg gatagaagtt
      121 cttggattct ttgaaggtag ggaaggaaga gctcgttctt cgttgcgttt ttgtcgaccg
      181 tagaaactag tgttttgctg tctgaaaaac agaaaatcgt ttcattctgt aagtcgtttt
      241 tctttagaaa ttctaaagtt tttaaaaaga cttcgaactc tgcttgatgg tttgtcccta
      301 tagaaagcgg gatagatagc tgttcttgtt taccttgata gacaatcaag attccacctc
      361 cactaggacc agggttgcct tttgtagagg catcaatgta tacttttaac ataaaaagag
      421 attctccttt ttgttcaaac taatgttatg gtaagattat atcattactg aatcggtgag
      481 aggttagaga atgaaaaaaa atattcgatg gcagcctgaa ctggcacata cgatcatcta
      541 ttggtcattg accttgatca ttctgttttt tagtctgatt ctttctttag aaaacacagg
      601 tccttattgg aaaagcaatt ttgttttagg tatttttctt gtttttctat ttctaggttt
      661 gcggcgttct tttattttaa aatcaaactc tgtgattata cgttatgctg ctttttggaa
      721 agaccgagaa atatctgtcg gacagattcg atctttgacg ccaactaata ctggggtgca
      781 aatgatgttg caagatgaga aagccccatc tgttttcatg atgcgaaaaa aagatcaagt
      841 agtccttttt gatcattttg aaaaaaacgg acccaaatca gaatcaattc cttatcctta
      901 tacgaaaaaa agaaaaagct aattttaaaa atcagaacct aaaaaaacga atttttacag
      961 gaaaagaaaa gagtgagacg tacggttcat cccgtttagc gtctcactct atttgtttat
     1021 ctattcatgc atactagtat cggtagtctg aacgttgcct ttcttgacat gggcagcttg
     1081 tgttcctcga ttcccttcga tgatctcaaa ttcgaccact tgattttctt ccaaagtttt
     1141 gaatccctct tcttcaatcg cagtaaaatg tacaaaaatt tcttctgtgt catcataact
     1201 gataaagccg tatccttttt tgttgtcaaa ccattttaca attcctgtgg tcaaagcact
     1261 cacgaccttt ctaaagtctg atcatagcct tcattataaa gagtatttat aagtacgtca
     1321 aataaattaa aaacct
//
LOCUS       DADJYG010000302         1333 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.313, whole genome shotgun sequence.
ACCESSION   DADJYG010000302 DADJYG010000000
VERSION     DADJYG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.313"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(175..645)
                     /locus_tag="JZ382_002833"
     CDS             complement(175..645)
                     /locus_tag="JZ382_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428958.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            732..>1333
                     /locus_tag="JZ382_002834"
     CDS             732..>1333
                     /locus_tag="JZ382_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAY2428959.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAV"
BASE COUNT          481 a          176 c          264 g          412 t
ORIGIN      
        1 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
       61 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
      121 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
      181 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
      241 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
      301 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
      361 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
      421 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
      481 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
      541 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
      601 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
      661 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
      721 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
      781 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
      841 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
      901 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
      961 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
     1021 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
     1081 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
     1141 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
     1201 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
     1261 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
     1321 tcgatgatgc tgt
//
LOCUS       DADJYG010000303         1314 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.314, whole genome shotgun sequence.
ACCESSION   DADJYG010000303 DADJYG010000000
VERSION     DADJYG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.314"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..848
                     /locus_tag="JZ382_002835"
     CDS             <1..848
                     /locus_tag="JZ382_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428960.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DADJYG010000304         1311 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.315, whole genome shotgun sequence.
ACCESSION   DADJYG010000304 DADJYG010000000
VERSION     DADJYG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.315"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..243)
                     /locus_tag="JZ382_002836"
     CDS             complement(<1..243)
                     /locus_tag="JZ382_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428961.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALE"
     gene            complement(691..1311)
                     /locus_tag="JZ382_002837"
                     /pseudo
     CDS             complement(691..1311)
                     /locus_tag="JZ382_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          331 a          282 c          243 g          455 t
ORIGIN      
        1 ttcaagagcc tcgtacatcg ctttgagatt gtttaaatcg ttgtgatcta acgcgttcgc
       61 taaaataatc cattggttca atttaggatc aaacatctga tcagcaatcg ttagcggttc
      121 gccatctgaa cgaacccctt cgagcggtgg ctgatctgtg taaggaacgg atacaagcat
      181 gtcgtccaat acttttcctg catactctcc gccagtacgg ccatatttcc aaatgttttt
      241 catttatttc cctcccagta attgaatttc ggtttccaat ttggaatcgg tggttcgact
      301 tctgtgcatt cttccggtaa atgttcttcg tcattcacaa tgatttgctc gaatccgtaa
      361 ggcttgattg atttgtaaac tactttcatt agatttcctc ccttaaattg aataagtgat
      421 aacaaatgaa taagctgatc cataacttga gtttcttcgc catttaatgg ctccatctgc
      481 accaatagat aactgagcac tgttcaaagt agaacggtct atcgagccaa ccagttgctc
      541 aaaactaatt ggtggccgat agccttctgg aattgttagt atcgttgaat catttccacc
      601 actgcttttc ccattcaacg caaaaaaata aatagtgacg gtttttcctt cacgataaag
      661 ctttgctgat ccggtattcc cgtttgtaac tgttaaggtt acgacttcat atttgttgtc
      721 gtctgatgtc aatacagatt ttccttttga ttgaatacca tcttcgaaat ttttcaatcc
      781 ttcgatggat tgcggttcag tcaaactaac cgtatcattc aagccttttt cagtatattc
      841 aggcgtgatg tcccagctgt agtcgttcgg attgttgcta tctttcaatc cttcaccaaa
      901 gtatttaaac tgactaatat tcggggttcg ggtgtcgcct ttttcaattt tgagccagtc
      961 aatttggcat gcgccggccg tttctttagg caactgaaaa atgcgaaggt ctttaggcaa
     1021 acccggttca agtttcgttg gtgtgaatgt tagagaccat acgtctgtca atccctcaac
     1081 tggttttagt tctccaaaat taacgttcca ataattatat gctacaaagg tttgacttgc
     1141 gggttttgtt cctttaagcg tgatagtata cgtttgacct attataagct cttcttccgt
     1201 gttaccttta tatatttcgt acgcgctaga tttaattggg aacgtcgtac ctttatttgc
     1261 aatattctca cccaacgatg ttttacctac ccagtaaggg tcatctagta a
//
LOCUS       DADJYG010000305         1301 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.316, whole genome shotgun sequence.
ACCESSION   DADJYG010000305 DADJYG010000000
VERSION     DADJYG010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.316"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(28..>1301)
                     /locus_tag="JZ382_002838"
     CDS             complement(28..>1301)
                     /locus_tag="JZ382_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAY2428962.1"
                     /translation="ETDQTKKLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYL
                     LEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFG
                     GNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEIT
                     ELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFL
                     FSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFST
                     LCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLL
                     IHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLD
                     TTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          418 a          180 c          236 g          467 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct t
//
LOCUS       DADJYG010000306         1274 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.317, whole genome shotgun sequence.
ACCESSION   DADJYG010000306 DADJYG010000000
VERSION     DADJYG010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.317"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..702
                     /locus_tag="JZ382_002839"
     CDS             <1..702
                     /locus_tag="JZ382_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY2428963.1"
                     /translation="IKKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPT
                     SVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLEN
                     MFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKII
                     AITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVE
                     LREKVLRSIIDKSIE"
     gene            932..1162
                     /locus_tag="JZ382_002840"
     CDS             932..1162
                     /locus_tag="JZ382_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAY2428964.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          447 a          191 c          208 g          428 t
ORIGIN      
        1 attaagaaaa acacaaaagt tggcgatgct actattattc gcttttgtca aaaaattggt
       61 ttttctggtt tttctgattt aaaaattgaa attgcaaagg aagactttag tcaaaaaaaa
      121 gaacatccga ctagtgtaaa gtactatgat gaaattgcta atagtttaac agaagctctc
      181 tattccacta ttaggctttt agacgaagaa aaattgaatg aagccattaa aatgatcact
      241 caatcaaaaa acatatatat atttggagtg ggatctagtg gaaatactag tcttgattta
      301 gaaaatatgt ttcttcgcgt aggagttcaa gcaaaagctg tactagaccc tcattttcaa
      361 gcccaagtcg cttctttatt aacagttaat gatttagtaa tcattttttc tttatctggg
      421 aaaacgaaag atacttatga ttcattgaaa atagctaaaa aaaatggcgc aaaaattatt
      481 gctataacta actatattca ttcacctata ggaaagtctg cagacttagt tttacaaaca
      541 gccattgaag aatttcttaa tggtggatct ttagctggaa aaatatcaca actttatatt
      601 tgtgatctat tagttcatgg ttatgaacaa aacaataata ttgattcagt tgaattaaga
      661 gaaaaagttt tgcgctctat tattgataaa agtattgagt gaactacccc cacttagcta
      721 aacctaacgg tttttaacga ttgaagtagg caattcttgg gatgagagca tacttgatag
      781 cttatttttt actaatagcg gtggctctta ttgaccaaat atccctgtag ttcctacggt
      841 tcttgatttt atctaaagat atttttatcg tggaagctat tgatcaactt gaaagaaaaa
      901 cgtgtagctc attatcacta gtttatcgag tatggatgaa gcaatggatc atcctctctt
      961 aaataaattt attaaggatc tgatccttca aattttttcc atgatcgatg agcaggaacc
     1021 tactgaatct aaacgacatc gggcacaagg aattattaag attgctaagc gaatgttttc
     1081 tataaagggt gttctaagct atacagtgtt aattcaaaag attcccaatc atcttgttta
     1141 taagaacaac gttgaagatt aaaaaagtgt ttcaatgcta aaattgcaaa atacttcaat
     1201 gttatgagac aaactgtcta taagaatcaa tttccaccca tttcatcact tgttaaaagt
     1261 ttatgatcct tttg
//
LOCUS       DADJYG010000307         1245 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.318, whole genome shotgun sequence.
ACCESSION   DADJYG010000307 DADJYG010000000
VERSION     DADJYG010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.318"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1244
                     /locus_tag="JZ382_002841"
     CDS             <1..1244
                     /locus_tag="JZ382_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY2428965.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DADJYG010000308         1235 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.319, whole genome shotgun sequence.
ACCESSION   DADJYG010000308 DADJYG010000000
VERSION     DADJYG010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.319"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..613)
                     /locus_tag="JZ382_002842"
     CDS             complement(<1..613)
                     /locus_tag="JZ382_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2428966.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..>1235
                     /locus_tag="JZ382_002843"
     CDS             901..>1235
                     /locus_tag="JZ382_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY2428967.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDS"
BASE COUNT          408 a          198 c          235 g          394 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattc
//
LOCUS       DADJYG010000309         1219 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.320, whole genome shotgun sequence.
ACCESSION   DADJYG010000309 DADJYG010000000
VERSION     DADJYG010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.320"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..492
                     /locus_tag="JZ382_002844"
                     /pseudo
     CDS             1..492
                     /locus_tag="JZ382_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="JZ382_002845"
                     /pseudo
     CDS             712..1008
                     /locus_tag="JZ382_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          442 a          226 c          258 g          293 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaa
//
LOCUS       DADJYG010000310         1195 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.321, whole genome shotgun sequence.
ACCESSION   DADJYG010000310 DADJYG010000000
VERSION     DADJYG010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.321"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..1076
                     /locus_tag="JZ382_002846"
     CDS             <1..1076
                     /locus_tag="JZ382_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY2428968.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DADJYG010000311         1158 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.323, whole genome shotgun sequence.
ACCESSION   DADJYG010000311 DADJYG010000000
VERSION     DADJYG010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.323"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..251)
                     /locus_tag="JZ382_002847"
     CDS             complement(<1..251)
                     /locus_tag="JZ382_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HAY2428969.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVP"
     gene            complement(262..>1158)
                     /locus_tag="JZ382_002848"
     CDS             complement(262..>1158)
                     /locus_tag="JZ382_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAY2428970.1"
                     /translation="QGTFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFES
                     SIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDN
                     DRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEA
                     IMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCER
                     SNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIH
                     QQEIRKQKRAMLFQQGRQQERE"
BASE COUNT          318 a          245 c          190 g          405 t
ORIGIN      
        1 ttaggaacat gatagtgata atgtgtaggg aaaataggca aatgctctaa tgaccattgt
       61 ttccaatttt catcgtacat atcgggcgaa tctaactcga ctaaatgacc aaaaccatac
      121 gtgatcacag tttcacccga aaacaatcga tcttggattt caaaataccc gtcttttcgg
      181 gtggctttag aaaagctatc ggcataggcc ttcgcttgtg acggtttttc tgctaaaatg
      241 acagttttca tcgtgacgat ctcactccct ttcttgttgt ctgccttgtt gaaacaacat
      301 cgcacgtttt tgctttctaa tttcctgttg atggatctca tgcttcaatt gatcgttcca
      361 atcctttttc acttcaggta caaattcatg ttcatgattt ttatagcctt tgtagtattg
      421 ttctgcttta taagagaaaa gaatctgaga attaatatcc ccttttcctt gataaaaacg
      481 atcaaaatca tacgtaccat tcgagcgttc acaatctttt aattcttttg cgacttctct
      541 acaaatatct ttcaaactaa tgacttctat tgaatcgtta acagaaagct gctgaccaaa
      601 agacgcattg actttgtgag cggaaaccag ttggttcgtt aattgcagat tgttttctgt
      661 cttgtgtatg gccataatag cttctggtgt gaccttttct tcttttgcga cttcttcata
      721 aatgactttg tattctcttg gcacttgcca atcattagcg attcctcgtt cacaacgtac
      781 tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt agaaaatatg
      841 tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt gtttctttct
      901 caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta acccttccat
      961 agagactaac cacgtatcgt ttaactgatc acgattcagt gcagcgtagc taagtaaatc
     1021 aatactactc tcaaaaaatt ttaactgttt aggatcacca atctttaggt taaagccatg
     1081 attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa aacgttcata
     1141 atcaatgaac gttccttg
//
LOCUS       DADJYG010000312         1152 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.324, whole genome shotgun sequence.
ACCESSION   DADJYG010000312 DADJYG010000000
VERSION     DADJYG010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.324"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            48..995
                     /locus_tag="JZ382_002849"
     CDS             48..995
                     /locus_tag="JZ382_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY2428971.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          377 a          173 c          250 g          352 t
ORIGIN      
        1 ttcggagctt caagatatct ataattattt atatcggagg taaaaggatg agctgttatc
       61 ttatcaccgg tggggctggc ttcattggtt ctaccttagc caatcattta ggagaagaac
      121 atgtggtaat cgtcgtcgat gacttatcaa tgggaaaagt agaaaatctt gatatgacga
      181 aaaatatcac ttttattgag ggagatgtcg ctgagaaatc cttgatggaa gagatcatga
      241 aagctaataa atttgattat atctttcatt tagcagcagt tgctagtgtg gcagattcag
      301 ttgcccgacc agtagaaaca catcgtgtga attttgaaag tgtattaatg attttagaat
      361 tgatccgcaa gtatcaacct gatttaaaac ggatcgtgtt tagttcttca gcggctgttt
      421 atggggatga accgacttta ccaaaaaaag aagagtccgt gattcgccca ttgacgcctt
      481 atgcggttga taaattcgct gcagaacaat atgtgctaga ttattgtcat ttatatgatg
      541 tacccggtag cgcagttcgt tttttcaatg tctatggtcc aaatcaaaat ccgaattctc
      601 catacagtgg tgtgatttct attctcgtag atcgctataa gaaacaaatc gtaggagaag
      661 caacttcatt tacattattt ggtgatggaa gccagtctag agactttgtt tatatagatg
      721 atgtaatcca agcattactt ttagttgcga atgaggaagc tgcactgggg aaacaattca
      781 atgttggaac gggtaaagca accacacttt tggaattgat tcatgcaatc aatgaaattc
      841 tcggtgtaga attgacactt gaatacaaag aagagcgttc gggtgatatc catgactctc
      901 tagcagatat tactaagatc cagtcaattg gctatcaacc gaaatatggt attttaagtg
      961 gaatgactaa ttatttgaaa actgaaatca attaaaacat actaagataa gattagaggg
     1021 taaattcagg ctctttgtca ataaggactg atgagttttg taaaatcaaa tctgggatag
     1081 aagggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattt ggacagaaca
     1141 gaaagaagaa aa
//
LOCUS       DADJYG010000313         1144 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.325, whole genome shotgun sequence.
ACCESSION   DADJYG010000313 DADJYG010000000
VERSION     DADJYG010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.325"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="JZ382_002850"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="JZ382_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY2428972.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          380 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccattttcat
     1141 ttta
//
LOCUS       DADJYG010000314         1128 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.326, whole genome shotgun sequence.
ACCESSION   DADJYG010000314 DADJYG010000000
VERSION     DADJYG010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.326"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..784)
                     /locus_tag="JZ382_002851"
     CDS             complement(<1..784)
                     /locus_tag="JZ382_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAY2428973.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYM"
     gene            complement(790..>1128)
                     /locus_tag="JZ382_002852"
     CDS             complement(790..>1128)
                     /locus_tag="JZ382_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAY2428974.1"
                     /translation="RNDIEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVP
                     TEVAFSDKTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKY
                     YELFELIQRG"
BASE COUNT          349 a          211 c          136 g          432 t
ORIGIN      
        1 tcatataatc tatcatcaaa taatccacca tcatatcatt atttttccaa taatttaaaa
       61 aaaagtctct aattatagca aacatccatc tagactcttg attacatgct aatgaataac
      121 ccgcaaaata tcctgatgct acggatgcgt gagcataatc aggcctccta atagaccaaa
      181 tatcgatatc aaaatactca tctggtattg gttttgtaca ataaaatgtg gcgtctaacc
      241 acaaacctcc atgttttgct agcaaagata atcttaataa atcagaataa tttgttctcg
      301 taatgattcc tttgtttttc ttttcttcca cccattttgg tatatcaaca tattgtctat
      361 aattatcttc tgttaaaatt ataacagtat gtttccctgc atttcgtttt attgatttta
      421 cacaagcttt tacaatttcc ggcgattgat ccaatccctg ccaccaacaa acccaaatgc
      481 agttagaata tggtgatttt gattgattaa ttatttgatt ttgactataa tcatatgttt
      541 tcaaaaaatc tgaaaaagtc ttttcataat acttcagcat tacctcatgt ttttttaata
      601 aatgttcctt ctgtttttta ctttcattat atccattacg attcatgact tgaatatcaa
      661 ttttagcttg taacgtggca aatgcttcct tccaagatgt tatctttgca attcgtcttg
      721 ttgcattcaa ttcttcggat atgcgatgta taattctttt tatacttgaa tgtttctgtg
      781 tcatattctt taacctcttt gtattaattc aaataactca taatattttg attctaagat
      841 tttgtatgct gtttgaatat tataatctgc ctttattaaa atcgaacgtt ctgaatctcg
      901 atgagagata tctacatttg caatctctct cgcccattct tttgccgatt gatttaaact
      961 tatgaaattg gtcttatcac tgaaagcaac ttctgtagga actttctccg aaaagaaaca
     1021 cggtaaatct gcaaattgag cttcaatacc cactaacgga accccttcaa ataatgatgg
     1081 caacattaat acgtcaaatg cttgataaaa tttttctata tcatttct
//
LOCUS       DADJYG010000315         1122 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.327, whole genome shotgun sequence.
ACCESSION   DADJYG010000315 DADJYG010000000
VERSION     DADJYG010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.327"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            99..884
                     /locus_tag="JZ382_002853"
     CDS             99..884
                     /locus_tag="JZ382_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAY2428975.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          436 a          170 c          180 g          336 t
ORIGIN      
        1 aagttgctat ataccctctt attttagcat actccctttt agaaaaagca aaaaaaaaag
       61 aaaaaatctt attcttgata acaagctacg tgacgtatat gcctaacaaa aatagaactt
      121 ttgaatcaat cttaaatgaa ggtttaacca cagaaccggc atccattatt tttgatgaag
      181 ttatcgatga cgttgaagat agtaagtatc ataaaaattt aaaagaactt ttttattttg
      241 cactcaacaa tggaccaagt ttgttcactg gtatatcagt acctgatagt gataatccat
      301 taattatggc aaaaatttac ttaacaaaat ggtgtaataa atacatcagg gataggaata
      361 acccagcatt aaaaaaacct cttaaaacat ttggcgaaaa agatgctgct ttaactacta
      421 gagtagcagc aaatgctaat attgatgatc aaaaagtatt gaatgactac ttgcgtggac
      481 attttttata tatgagcgct gagaacatga acggaagtat tttagaggaa tatttagccg
      541 aagtgttaga acctgaaggt tggatttggt gcgccggctc tgtatatcga gctgttgatt
      601 tctgttattt aggaacctca ccaattcttt tacaagtgaa aaataaatac aatacagaaa
      661 gcagttcgag ttcagctata agagtaggaa caacaattag aaaatggaat cgtttaaata
      721 agtcgacaaa aatttctggt ttggatagcc ctatccccaa ttggaaagca ttaatagaaa
      781 tgactgaagc tagtaaggaa ctggcagcta agttaactga aaatagttat ttagcttata
      841 ttaatgaaaa atccacacga gaattatgga cactcgatga ctaacaatat ccttattagt
      901 atctatccac gatacatgga aaaaattata aacaaacaaa aaaactttga atttagaaat
      961 ttctatctta cctcagatcc ttataatatc ttttggattt acgaaacaat ccccaccaaa
     1021 gctttaaagt ataaattgat tgtaaaaaat ccaattacta aacttaagaa aaatcaacac
     1081 tactttttag gagacgatga gtttgaacaa ttagtttaaa ag
//
LOCUS       DADJYG010000316         1115 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.328, whole genome shotgun sequence.
ACCESSION   DADJYG010000316 DADJYG010000000
VERSION     DADJYG010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.328"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..898)
                     /gene="vanY"
                     /locus_tag="JZ382_002854"
     CDS             complement(<1..898)
                     /gene="vanY"
                     /locus_tag="JZ382_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAY2428976.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          400 t
ORIGIN      
        1 tagtatgtgt tgatccggga aacactgtca caattacacc gtctatattg tttcctgata
       61 tctcataacg aagattagtc ggcacatgaa tggtggtatt tttagtaaca ggataataaa
      121 agatctcata tttttcccca tttacactaa cagaaatggt tttttcttct tttaggtaat
      181 ccatatattc ctcgagaacg aaattctttt ctttcataat cgcactatgt ggtaaaccaa
      241 catagcgaat atgccatggt tcatattgaa ttcctgttaa ctctgttttg tcctctggat
      301 aacgtaaaat gaacccgtat ttccaagcat tttcttctat ccactttcct tcaggggctc
      361 gttccatttt cgtcaagctt gatcctacat ctagtgataa acctgaatta tgctcactat
      421 aacctgctgg taaggcatac tcagccccca tttcttggta aagcacactt tgctcatcaa
      481 agtctcgata gccactatta ataataaaat gactaacgcc accctttaca gcatcattga
      541 ccatctctga aaatttttgt gctatttctt ttgacatata aatattacta tcaagcaacc
      601 cgtatccatt tattaattcg tcatgtttag ataaattcac gatatctgac ttcacacttt
      661 cttggcgaac aggatattta ctattgatta atagcagatt tccttgataa acctgttctt
      721 ctgtaattgt tttctcttgg gtattttcag aagtcccact attttctaaa tgttctttgg
      781 gattttgatc ataattttga aattcgactt tttcctgaga aaacagtgct tcattaacgt
      841 agtcataacc taagtatatt aagaataaca ataacaataa aaaaaacaac ttcttcattt
      901 tcagtctcct ccttaactat atagttggaa gtatgaaata tttctagaat cttcccctta
      961 aacggcacaa tctcgtttga gattgtgccg ttttgccccg taaattacag tcaattattt
     1021 cgccctactg gatttactat tatcaccaat gtagatgaat tttcataagt gatacccatg
     1081 ctgaatttaa cgaggctgaa ctgcctgttg acaat
//
LOCUS       DADJYG010000317         1110 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.329, whole genome shotgun sequence.
ACCESSION   DADJYG010000317 DADJYG010000000
VERSION     DADJYG010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.329"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(85..1041)
                     /locus_tag="JZ382_002855"
     CDS             complement(85..1041)
                     /locus_tag="JZ382_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428977.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          362 a          182 c          167 g          399 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taatattttt agaaaaagca
       61 gtcggacttt ctaaaagaag ttcactattc gatcctgtcg atgatttctt caggaaaatc
      121 gtctatttgc ttgtcaattt tgctgtcttt gtatttctct tcttggatag tcaacaaaat
      181 ctttgtaaga ttcccccagt ctactgctct tggcggatcg agccaaacca ctacttgaga
      241 tgcactttct tcatatagat tattcctatt cgtgattata acatccggga actcaggctt
      301 tcctgcctct atcacttccg tgttcagatt aatgtacaag cttaaatttt tcttgataaa
      361 ttgattatag gcagcaccat aagaaaaatc gatgatgatc tttactggtt cagaaatcag
      421 ttgaatgggt atgtgattga tcaataataa taaataactg aaaaaaagaa aaaatttatc
      481 ttgaagcaac tctttgcgtt ttgacgttag atcatttata tactctatca aatagcaata
      541 aaatgctgga tagttttctc taaaaaaaga aacatcaagt ggaaattcat cagctttatt
      601 aatagtgtaa tgcaaaagtt taaaatgtat tttggttaac tctcttcgga tcaattggct
      661 cgtctcttct cgcaacagat taaactgatt ttcaaaactt tcaatgaatc gttcattcaa
      721 ccattcgatt gtttcatctt cattttttct caagtatttt tgatccaatt tatcatgaag
      781 ataaagccaa gagagtgtct tattgataaa aataacggat ttttctccta atactccttt
      841 taagtggtaa aatcctatat ccgaaaaaaa tagcgactga ttgctagcaa tatctaaaga
      901 atcagcttgt tcagccaaca ggttgattgc taaaaagtgg aataatttct ttttttcata
      961 agcagacaga ggataatact gttttaaatc ttttaacatt ttttctgttt ttataattat
     1021 aggtagcgta tacaattcca caaaacttat ctctgttgag ttaaaaagat tatataaaaa
     1081 aagccgaatg tttttaggat tccctgtcaa
//
LOCUS       DADJYG010000318         1103 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.330, whole genome shotgun sequence.
ACCESSION   DADJYG010000318 DADJYG010000000
VERSION     DADJYG010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.330"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..1052
                     /locus_tag="JZ382_002856"
     CDS             12..1052
                     /locus_tag="JZ382_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAY2428978.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          452 a          165 c          188 g          298 t
ORIGIN      
        1 gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat
       61 tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa
      121 ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa
      181 tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa
      241 actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat
      301 tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc
      361 tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga
      421 atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat
      481 cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg
      541 ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta gacactaata
      601 gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg
      661 agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga
      721 taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg
      781 caaagaaaaa agctgaaata gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt
      841 attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa
      901 aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta
      961 tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc
     1021 caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt
     1081 catggagtaa cattaaaatt aaa
//
LOCUS       DADJYG010000319         1085 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.331, whole genome shotgun sequence.
ACCESSION   DADJYG010000319 DADJYG010000000
VERSION     DADJYG010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.331"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            17..277
                     /locus_tag="JZ382_002857"
     CDS             17..277
                     /locus_tag="JZ382_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428979.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(639..878)
                     /locus_tag="JZ382_002858"
     CDS             complement(639..878)
                     /locus_tag="JZ382_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428980.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          383 a          160 c          198 g          344 t
ORIGIN      
        1 taaggatgga tgaaatatga ataacgaaac tatcatttta ggaaaaaaat atatttgcca
       61 acctattggc ttgaaacaaa aagtggttgg agaagtaatc agtaaattag aaaattgtgt
      121 tgtgatctgc gtagacgaat atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg
      181 aaaagtagtg gcaaaatata ctcacgttta tgagttggaa acaacagaat atctatataa
      241 ctatagcaac aaatatgaac atactttagt ttcataaaga tttaaataaa gtgccttcaa
      301 aagcgagaag accaagcttt tgaaggcact ttatttagtt ggcagtgatt gttcatgatt
      361 tttgatttat tcaaaaggtt cgcctagaga atctgtctct gtaacgaggc ctggtggaga
      421 aactcgataa aaacctatac tgccagaact gtcctatttt cggatagaca aactagtagt
      481 tttaaaaaga tcatccgatc caatcatttc aaaaaatgca aaactgatat caggatcatt
      541 tagctcaggc ttgttatcgt aaagcatttg taaaggttgc atttagttag gagtaaacgc
      601 gaaactatca tcattatttg ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa
      661 taaaggagac gtgctgacag tttctttacc ctgttttttg cagaggtaga aacttttata
      721 tcggaagttt ctaccggaga atttttctca tttgtagaaa ttgttgaatc gatactattc
      781 gaaatttgcg tatctgaagg tatgttcttt gatgcagaag tcccacaagc agtaagtaac
      841 aataaatttg ataaactgat aaacagaatt cttttcatta tgtacacact ccttatagta
      901 tcgtttagca tacacctatg ctcagtatta ggcaaaatca atgcaaaact atcaagtctt
      961 ttatagtatc tattagtctt agtaatacat atatggagag ggtgatatcc cagttcaaac
     1021 cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt ttttatgaaa
     1081 aaatt
//
LOCUS       DADJYG010000320         1069 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.332, whole genome shotgun sequence.
ACCESSION   DADJYG010000320 DADJYG010000000
VERSION     DADJYG010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.332"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..969
                     /locus_tag="JZ382_002859"
     CDS             <1..969
                     /locus_tag="JZ382_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY2428981.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DADJYG010000321         1056 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.333, whole genome shotgun sequence.
ACCESSION   DADJYG010000321 DADJYG010000000
VERSION     DADJYG010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.333"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            423..1056
                     /locus_tag="JZ382_002860"
                     /pseudo
     CDS             423..1056
                     /locus_tag="JZ382_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          365 a          183 c          188 g          320 t
ORIGIN      
        1 aaatgtgatt aattttatta ttaataaaat attttttata ttacaaaagt agtgtttcaa
       61 agttagtaga gaaaatgtag ttatatattt aaatttttac cagaaagaaa tgaacacctt
      121 caattatcta aatctcgaaa atggttagat gtaatcatca ggaaaaacaa tcttaaacga
      181 attactattc atggtcttag acacacacat gctagcttgc ttttagcaag ctagtgcaaa
      241 tattaaagat gtgcaagagc gtttaggcca ctcttctatt caaatcatta tggatctata
      301 tattcatatt acagacaaaa gaaaagaaga aaccgtagcg caattcgcaa aatataggct
      361 ctataattta tgggactgat gaatcaaaat tgattcttca gtcccattgt tattttaggt
      421 atatgattcg tacattgaaa gagttgaaaa actatcttcc gaagccgtca aaaaggaccc
      481 tgccaaaagt attgatggtg gatgaatttc gttcgcacgc ttcaattgtg gataaaatga
      541 gctttatttg tgcggatggc gagactggaa aactcattga tattttgcct acacgaaaat
      601 tgtctcgatt aacacactat tttagtcgct gtgcaaatcc tgaagcggtg gaatatttag
      661 tgacagatat gaacgccgcc tacttccaac tgacaaaacg tgtcttacca aatgcgaaag
      721 tcgtcattga ttgttttcat atcatcaaac atttgaatca ggcgttcaat gattttcgtg
      781 tacaagaaat gaaaagatta caccagttag ggcaacgacg ggaagccgag aaagtgaaaa
      841 agaactggcg ctttcttcta aaaaaccgtc aaaaaatcaa tcactatgat tacaaaacat
      901 ggcgcagttt tcgtgcacct aaatacccgc tactcactga agcaatgatg attgatcgat
      961 tgctggcatt ttcaacgcct ttaaaggacg cctatacttt tttccatgag ctgaccgaag
     1021 cctttagaga caaggaatca gacttatttt ttacct
//
LOCUS       DADJYG010000322          995 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.334, whole genome shotgun sequence.
ACCESSION   DADJYG010000322 DADJYG010000000
VERSION     DADJYG010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.334"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(26..913)
                     /locus_tag="JZ382_002861"
     CDS             complement(26..913)
                     /locus_tag="JZ382_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAY2428982.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DADJYG010000323          991 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.335, whole genome shotgun sequence.
ACCESSION   DADJYG010000323 DADJYG010000000
VERSION     DADJYG010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.335"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(21..94)
                     /locus_tag="JZ382_002862"
     tRNA            complement(21..94)
                     /locus_tag="JZ382_002862"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="JZ382_002863"
     tRNA            complement(108..181)
                     /locus_tag="JZ382_002863"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="JZ382_002864"
     tRNA            complement(209..281)
                     /locus_tag="JZ382_002864"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="JZ382_002865"
     tRNA            complement(301..374)
                     /locus_tag="JZ382_002865"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="JZ382_002866"
     tRNA            complement(384..457)
                     /locus_tag="JZ382_002866"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="JZ382_002867"
     tRNA            complement(468..553)
                     /locus_tag="JZ382_002867"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="JZ382_002868"
     tRNA            complement(570..641)
                     /locus_tag="JZ382_002868"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="JZ382_002869"
     tRNA            complement(653..725)
                     /locus_tag="JZ382_002869"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="JZ382_002870"
     tRNA            complement(743..824)
                     /locus_tag="JZ382_002870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="JZ382_002871"
     tRNA            complement(832..904)
                     /locus_tag="JZ382_002871"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="JZ382_002872"
     tRNA            complement(908..980)
                     /locus_tag="JZ382_002872"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          284 c          235 g          212 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgcttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DADJYG010000324          977 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.336, whole genome shotgun sequence.
ACCESSION   DADJYG010000324 DADJYG010000000
VERSION     DADJYG010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.336"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(55..789)
                     /locus_tag="JZ382_002873"
     CDS             complement(55..789)
                     /locus_tag="JZ382_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAY2428983.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          339 a          201 c          181 g          256 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaa
//
LOCUS       DADJYG010000325          965 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.337, whole genome shotgun sequence.
ACCESSION   DADJYG010000325 DADJYG010000000
VERSION     DADJYG010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.337"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(61..279)
                     /locus_tag="JZ382_002874"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="JZ382_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="JZ382_002875"
     CDS             complement(545..739)
                     /locus_tag="JZ382_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428984.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          329 a          166 c          151 g          319 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaag
//
LOCUS       DADJYG010000326          959 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.338, whole genome shotgun sequence.
ACCESSION   DADJYG010000326 DADJYG010000000
VERSION     DADJYG010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.338"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(107..511)
                     /locus_tag="JZ382_002876"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="JZ382_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="JZ382_002877"
     CDS             complement(570..>959)
                     /locus_tag="JZ382_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2428985.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DADJYG010000327          956 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.339, whole genome shotgun sequence.
ACCESSION   DADJYG010000327 DADJYG010000000
VERSION     DADJYG010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.339"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            62..>956
                     /locus_tag="JZ382_002878"
     CDS             62..>956
                     /locus_tag="JZ382_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAY2428986.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          321 a          181 c          190 g          264 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcaccac aaaataaaca actgagagga cgatacgcta
       61 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
      121 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      361 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      481 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      541 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      601 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      721 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      781 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatg
//
LOCUS       DADJYG010000328          952 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.340, whole genome shotgun sequence.
ACCESSION   DADJYG010000328 DADJYG010000000
VERSION     DADJYG010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.340"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            218..529
                     /locus_tag="JZ382_002879"
     CDS             218..529
                     /locus_tag="JZ382_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428987.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            541..>952
                     /locus_tag="JZ382_002880"
     CDS             541..>952
                     /locus_tag="JZ382_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2428988.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFI"
BASE COUNT          428 a          128 c          155 g          241 t
ORIGIN      
        1 ttcatgattg aaaaattatt agatagatat attgcaaagt ctgaattaga accacacaat
       61 atcaattaca tgaaatttat agaagataac aatttaagcg ttcgcttcga accacttgtg
      121 aaagaattat taaaagctcg catctattgt tatgcagctc tctaaaattt tttaagcaaa
      181 aaagaacata cgttcaaaaa tagaaaggtg aacaaaaatg atatatacag aattcaaaga
      241 atggttagaa aaaagcacaa ccggatacga aacatttatc atcaaagcta ctaattatca
      301 aattgaaaaa aacaaaaata gaccccaaaa aaaacgctgg gatgataaga aaatagataa
      361 agctgtatta gaaatgtgga aacaagtcgt gactaacttg tatcaaacaa ttcgtaaaga
      421 aaaaggagtt ccattaatta acgggaagga aatatggctt gaatttatag aggaacaagg
      481 actgatcgaa tttttcaatg atagcatggc agaattagaa tttgaatagg ggtaatattg
      541 atggcaatga taaaacaata taaaaagaaa aatggcgaaa aagcatggta ctttaaaact
      601 tatctcggta ttgatccgct aactggaaag aaaaaatata ctactaaacg aggatttaga
      661 acacaaaaag aagcaaaaac agcactttct aggttagaac tagaattaca aaaaacagga
      721 atgcccacaa gtacaaatac tactttcaaa gaagcagcag aattatggct agaaagctac
      781 aaaaaaactg taaaagaaag ttcatattca aggactaaaa taatctttaa taaacatata
      841 tatcccaaat ttggaaatat taagctttct aaaattaata cggcatattg tcaaaaggta
      901 gtaaatgatt ggagtgaaaa aggaacttca aagcagtacc ctcttttcat aa
//
LOCUS       DADJYG010000329          942 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.341, whole genome shotgun sequence.
ACCESSION   DADJYG010000329 DADJYG010000000
VERSION     DADJYG010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.341"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            12..422
                     /locus_tag="JZ382_002881"
     CDS             12..422
                     /locus_tag="JZ382_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAY2428989.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="JZ382_002882"
     CDS             436..792
                     /locus_tag="JZ382_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAY2428990.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          318 a          153 c          186 g          285 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta gg
//
LOCUS       DADJYG010000330          932 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.342, whole genome shotgun sequence.
ACCESSION   DADJYG010000330 DADJYG010000000
VERSION     DADJYG010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.342"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..162)
                     /locus_tag="JZ382_002883"
     CDS             complement(<1..162)
                     /locus_tag="JZ382_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAY2428991.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEY"
     gene            371..583
                     /locus_tag="JZ382_002884"
     CDS             371..583
                     /locus_tag="JZ382_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428992.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            745..>932
                     /locus_tag="JZ382_002885"
     CDS             745..>932
                     /locus_tag="JZ382_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2428993.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          322 a          155 c          154 g          301 t
ORIGIN      
        1 atattcttgc acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa
       61 gatttcttga acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat
      121 aatcacatcc tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgctat
      181 ctttttctat tattctacct tatttgatag tagatttaaa actttgcaac agaaccctaa
      241 aaacactata tattccgaaa agtgagtatc tccattcata aaataatcaa catatttaaa
      301 aaatctaatt agctttttta gtcaatctaa agttctttat tctatgatat aatgttactg
      361 tagaaagttt gtggcggttt ggcaaatcca attgaaggag cgtggtgcct atgggcttat
      421 tagcttatcg gacgtactct gtagaaagga aatgcctgat ttgttctcat tggatcaaat
      481 cattgctatt atcggagcag cagcagtagt tattactgcc gtagcgaaag ctagcgattc
      541 gattttagct tggttaaagt tcaaaaaaga acaaaaaaaa tagagccgtc tcaacttttg
      601 gcgaggtaga cgagctctaa agctttattt acatgccatc cgctttgaac ggactacact
      661 gggcatgtga cagcatgccc tttcatttat attatatctc attcttttat ataatgaaag
      721 taaataaatc atggaggata aaatatggat aaatttaata gattaacatt aataaatcag
      781 tttgaaattt taaaagcttt aaatcctaac gaagaaaaat actatgctga aaaaatagaa
      841 atactaacag aaggttacga atatcactat agtgaaatat ttgaaaatat ttctgatcca
      901 ttgccagaag aagattcgcg gtttgttcta ga
//
LOCUS       DADJYG010000331          922 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.343, whole genome shotgun sequence.
ACCESSION   DADJYG010000331 DADJYG010000000
VERSION     DADJYG010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.343"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            24..>922
                     /locus_tag="JZ382_002886"
     CDS             24..>922
                     /locus_tag="JZ382_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAY2428994.1"
                     /translation="MEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAK
                     SNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLHLRDS
                     VRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKK
                     IENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQM
                     EVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPL
                     GSGMQYESSVSLGYLNQSFQNAV"
BASE COUNT          328 a          142 c          180 g          272 t
ORIGIN      
        1 aatctgaaca atgggatacg gtaatagagg gaaacgatga ccttttagag aaatatatgt
       61 ccggtaaatc attagaagca ttggaactcg aacaagagga aagcataaga tttcagaatt
      121 gttctctgtt ccctctttat catggaagtg caaaaagtaa tatagggatt gataacctta
      181 tagaagttat tactaataaa ttttattcat caacacatcg aggtccgtct gaactttgcg
      241 gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg tcttgcatat atacgccttt
      301 atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa aaaataaaag
      361 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
      421 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      481 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      541 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      601 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      661 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      721 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      781 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      841 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      901 aatcatttca aaatgcagtt at
//
LOCUS       DADJYG010000332          912 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.344, whole genome shotgun sequence.
ACCESSION   DADJYG010000332 DADJYG010000000
VERSION     DADJYG010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.344"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            157..543
                     /locus_tag="JZ382_002887"
     CDS             157..543
                     /locus_tag="JZ382_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY2428995.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            637..828
                     /locus_tag="JZ382_002888"
     CDS             637..828
                     /locus_tag="JZ382_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2428996.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          339 a          140 c          142 g          291 t
ORIGIN      
        1 atcaactaaa aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct
       61 tatagaagtt attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc
      121 ttttcccttt aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta
      181 aatgcccagt tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat
      241 aaaaaacaag aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt
      301 actaaagata ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt
      361 gaagatataa aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt
      421 atcgaaaaat ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca
      481 agacagggtg cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt
      541 taatatctgc ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag
      601 aaatagatta taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc
      661 attttagcaa aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagctct
      721 ttgattcata agcaatcctg gagaactgga aaatatgatc aagattgtta tcaagaatgt
      781 atgcttgcaa tatatctagc tatctcgaaa ttcgaaatta aagaataact caactaaggg
      841 agtctgtgac ataagtaaaa agatagtttc aaatttcgaa caataaattt gaaactatct
      901 tctttataat ta
//
LOCUS       DADJYG010000333          912 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.345, whole genome shotgun sequence.
ACCESSION   DADJYG010000333 DADJYG010000000
VERSION     DADJYG010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.345"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>912
                     /locus_tag="JZ382_002889"
     CDS             <1..>912
                     /locus_tag="JZ382_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAY2428997.1"
                     /translation="YISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLI
                     LKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVV
                     GWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNE
                     TAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCA
                     SGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVV
                     RKRAVGKDIFIKHYHIVVESWGLLFVQ"
BASE COUNT          285 a          115 c          178 g          334 t
ORIGIN      
        1 attatatatc ctatgtatgg aatcaatttc taatacaatt tgctaaaggg ttagaaaaag
       61 ttaaagctat gggcatagca ggtattatca gcacaattgt aacaataatt gccaatattt
      121 tcttcttgct tattctgaaa tggggattaa ttggtttttt cattgcaaat attttatcac
      181 agattatttc agctacttat ctgtcagtat tagtgagaat atgggaatat atcgattttg
      241 aggctaaaga tcctcagcta aagagagaaa tgctaaatta ctcgattcca ttaattgcaa
      301 ctgttgttgg atggtggatt aatagtacag ctgataggta tgtggtttcc tttatagtag
      361 gtgtctcggc gaatggctta ttgtctgtgg catataaaat acctcaaata attaatactt
      421 tgcagggaat atttatacag gcctggcaaa tttctgcaat taaagaatat ggagataatg
      481 agactgcaaa attttatgga aatacatttg ttatgattaa tttgttaatg tgcataacct
      541 gttcaagtct tatcatatta actagaccgt tttcacatat tctttatgcg aaagactttt
      601 atgaagcgtg gaaatatgtt ccatttttat taatagctaa tgttttaaat tgtgcatcag
      661 gtctgttagg acctatctta tcagcaaaaa aagattctaa atccatgatg tggtctgcaa
      721 tcataggggc attagttaat gtgattatga gtgtagtcct tgtttataag ataggggttc
      781 aaggtgccac aatagccact gttatatgtt catatgtaat atatgttgtt cgtaaacgag
      841 ctgtgggaaa agatattttt ataaagcatt atcatattgt tgtggaatct tggggattat
      901 tgtttgttca gg
//
LOCUS       DADJYG010000334          907 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.346, whole genome shotgun sequence.
ACCESSION   DADJYG010000334 DADJYG010000000
VERSION     DADJYG010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.346"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..888
                     /locus_tag="JZ382_002890"
     CDS             <1..888
                     /locus_tag="JZ382_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAY2428998.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          119 c          149 g          250 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcag
//
LOCUS       DADJYG010000335          877 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.347, whole genome shotgun sequence.
ACCESSION   DADJYG010000335 DADJYG010000000
VERSION     DADJYG010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.347"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            87..356
                     /locus_tag="JZ382_002891"
     CDS             87..356
                     /locus_tag="JZ382_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY2428999.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="JZ382_002892"
     CDS             349..606
                     /locus_tag="JZ382_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAY2429000.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          333 a           97 c          168 g          279 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgctt
//
LOCUS       DADJYG010000336          876 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.348, whole genome shotgun sequence.
ACCESSION   DADJYG010000336 DADJYG010000000
VERSION     DADJYG010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.348"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            217..>876
                     /locus_tag="JZ382_002893"
     CDS             217..>876
                     /locus_tag="JZ382_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HAY2429001.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLD
                     SK"
BASE COUNT          318 a          105 c          164 g          289 t
ORIGIN      
        1 caaaaattat tggcaaatga gatttggagt agtttagaag tcgaagaaag taaacaagaa
       61 cttttgttga aatggatact ttttcttgta aagaagttct atgtgaaaat accagaaacc
      121 cattgtctga tagttatgca cggtaaaata acagcttctg cgatcgcaag ggaaacaaat
      181 aagttgctga atacatatgt ttatgaggct ttcgatatgc caattgaagg tgagactagt
      241 gacttaatta acctaatcaa caatttttgt aaaagtatag atacgacgaa tggactcatt
      301 ctgctagttg atatgggatc attggaacaa atgtatgaaa aaattgaatc caatgtaata
      361 ggtgatttgg taattttaaa caatgtttct acagctttag cgattgaatg tgggattcaa
      421 gtttgccaaa aaaagccaat tagtcatttt taccagatgg attttgattc gtttcaagtt
      481 aaagttcagt attataaagg attatcacaa aagaaaaatg taatagtatc ttgtctctct
      541 ggtgagggga tttctgaaaa agttaaggat attttaaaac ggtatctcga taatcaagtg
      601 gagattttga tttttgattt taacgcatta aagaaaatag caaaggaaaa agatactatg
      661 atttttaaga atacaatttg tgttctttca accactgaat tttacattca aggaatcgat
      721 tgtttaaatt tagaaaatct aattaatggg aatcaaacct tagaaaaatt aaataagtat
      781 atggactcgg atagctgtga gttctgctta aatgaattag ttaaactctt tactattgaa
      841 ggcgctagta ctaagcttcg ctttttagat tccaaa
//
LOCUS       DADJYG010000337          876 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.349, whole genome shotgun sequence.
ACCESSION   DADJYG010000337 DADJYG010000000
VERSION     DADJYG010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.349"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..75
                     /locus_tag="JZ382_002894"
     CDS             <1..75
                     /locus_tag="JZ382_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAY2429002.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            253..594
                     /locus_tag="JZ382_002895"
     CDS             253..594
                     /locus_tag="JZ382_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429003.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            587..>876
                     /locus_tag="JZ382_002896"
     CDS             587..>876
                     /locus_tag="JZ382_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAY2429004.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          325 a          142 c          185 g          224 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacggaccag gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaatcaa
      601 agggacaacg aactttgaaa aactttttag tcgtaagtta aataaaattc tcaagaaaaa
      661 aggaaatttt gattatttat cttgggctca cgcgtgggag attatgaaaa agaatgatcc
      721 acaggcaacg gtaactatta atgagtacaa acactacagg gttgtttctg gaactcatca
      781 agactttctt gttgaggaat ataaaccttt tcttatggat gaaactggga cttatgtatc
      841 tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DADJYG010000338          872 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.350, whole genome shotgun sequence.
ACCESSION   DADJYG010000338 DADJYG010000000
VERSION     DADJYG010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.350"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..75
                     /locus_tag="JZ382_002897"
     CDS             <1..75
                     /locus_tag="JZ382_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAY2429005.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..590
                     /locus_tag="JZ382_002898"
     CDS             249..590
                     /locus_tag="JZ382_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429006.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            583..>872
                     /locus_tag="JZ382_002899"
     CDS             583..>872
                     /locus_tag="JZ382_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAY2429007.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          335 a          138 c          176 g          223 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg
      601 acaacgaact ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga
      661 aattttgatt atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag
      721 gcaacggtaa ctattaatga gtataaacac tacagggttg tttctggaac tcatcaagac
      781 tttcttgttg aggaatataa accttttctt atggatgaaa cagggactta tgtatctgtc
      841 tcagtaacgg ttaaaggaca tacggaaaca ga
//
LOCUS       DADJYG010000339          859 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.351, whole genome shotgun sequence.
ACCESSION   DADJYG010000339 DADJYG010000000
VERSION     DADJYG010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.351"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>859
                     /locus_tag="JZ382_002900"
     CDS             <1..>859
                     /locus_tag="JZ382_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAY2429008.1"
                     /translation="RNFDYEISYNEVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGIA
                     DYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKK
                     LLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFD
                     YQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLN
                     SVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAIDMNK"
BASE COUNT          296 a          116 c          158 g          289 t
ORIGIN      
        1 aggaattttg attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat
       61 aagttgaatt ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc
      121 gctggtatag ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg
      181 gctgggctaa atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat
      241 gtatctcctt ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct
      301 aaaaaattgt taaaaaatat caatttagca aatataaatt atagtgacga acttccttta
      361 tcccctttac attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa
      421 gatggacttc atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac
      481 tatcaattat ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat
      541 ttttcaaatc aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct
      601 ggagatttat cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta
      661 tctggagatt cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa
      721 caacaaaaag gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct
      781 tgttgcaatg ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact
      841 gcaattgata tgaataaag
//
LOCUS       DADJYG010000340          858 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.352, whole genome shotgun sequence.
ACCESSION   DADJYG010000340 DADJYG010000000
VERSION     DADJYG010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.352"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            146..379
                     /locus_tag="JZ382_002901"
     CDS             146..379
                     /locus_tag="JZ382_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAY2429009.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            376..783
                     /locus_tag="JZ382_002902"
     CDS             376..783
                     /locus_tag="JZ382_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAY2429010.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          322 a          118 c          154 g          264 t
ORIGIN      
        1 agcaatctaa attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata
       61 gagttagaca tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat
      121 acaaagaaaa aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg
      181 gaactcagta gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg
      241 agatacggtt tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt
      301 atctgatgaa attgatttag caatatctca agcattaact gaatatgata ctacttttag
      361 agagcttgtc gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat
      421 gtaatccaaa taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct
      481 ttagaaatgt gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg
      541 atttatgaaa aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat
      601 gcaaataaac gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt
      661 ttagctattg atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag
      721 gatttcgatc aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt
      781 taaggagtta ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag
      841 taaattacaa atagctat
//
LOCUS       DADJYG010000341          847 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.353, whole genome shotgun sequence.
ACCESSION   DADJYG010000341 DADJYG010000000
VERSION     DADJYG010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.353"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..329)
                     /locus_tag="JZ382_002903"
     CDS             complement(<1..329)
                     /locus_tag="JZ382_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429011.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALG"
     gene            complement(464..>847)
                     /locus_tag="JZ382_002904"
     CDS             complement(464..>847)
                     /locus_tag="JZ382_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAY2429012.1"
                     /translation="SNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVV
                     RDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMR
                     DELSELAKKVKVTFLTDPRWVRIDK"
BASE COUNT          293 a          172 c           77 g          305 t
ORIGIN      
        1 cctagagcaa atgataatat ttcttttaca aagctgttca atgaaaatgt ttctattcca
       61 catgcacaaa gaattccata aactaataca ctataaaaaa tacattctaa tacaattccc
      121 aaaacatttt caaagtgaat gtttcttttc tcacttaaaa aatatgccga aataaatata
      181 aaacaattaa ctcctagctg accccaaata ccaatataat tcaagaaaat ctggttagca
      241 gttaatgaac catgtactat aattccgtct gcattataat ttaaacgcaa attagcatgg
      301 taagcaacaa tacacaatat agaaatgatt cttaaaagtt caaaattaga gtttcatctc
      361 gccccatcgt cccttaaata tttttcttct tttttattta agactttacc catttttatt
      421 ctctcactat ctctttatca aaattttaaa aacattatta ctactactta tcaattcgaa
      481 cccaacgtgg atccgttaaa aatgtcacct ttactttttt agctaattct gacaattcat
      541 ctctcatttg cacgttcatt gcatctaata atgcaaattt tgttataatt tccatggatt
      601 gctcaccata attctcatct tcattatgtg aatatccatc aaatccagct aagaatattt
      661 catccacatt catatcggct aaaaatctaa ttgccattaa tcctgcattg tctcttacaa
      721 cttccctttc agataacaac tttttataac tcgtccgtaa atatacatcc gatgcttgta
      781 tatttgatgt aacaatacat ttagttctgc tttctttcgg taattcatga aaacgtctta
      841 aattact
//
LOCUS       DADJYG010000342          811 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.354, whole genome shotgun sequence.
ACCESSION   DADJYG010000342 DADJYG010000000
VERSION     DADJYG010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.354"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="JZ382_002905"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="JZ382_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAY2429013.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DADJYG010000343          809 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.355, whole genome shotgun sequence.
ACCESSION   DADJYG010000343 DADJYG010000000
VERSION     DADJYG010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.355"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..805
                     /locus_tag="JZ382_002906"
     CDS             <1..805
                     /locus_tag="JZ382_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429014.1"
                     /translation="LFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLF
                     NHTPVKSLQFVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGV
                     SNSIHANKDSLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPT
                     THFMSNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPG
                     INKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT          272 a          130 c          103 g          304 t
ORIGIN      
        1 attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat taactgctga
       61 tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca atttatttcc
      121 ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc ggttaaatgg
      181 tttttcttct ttatttatag cctatacgat gagtatagtg atctattata ttttttctac
      241 taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat tgcatctctc
      301 aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag cacctgaaga
      361 cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag agtcaatcta
      421 tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa taaatccaac
      481 aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata ataaaaatac
      541 tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta atggcaaact
      601 agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc caggtataaa
      661 taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat atttatctat
      721 cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag
      781 aagagaagta caacattctc actagattt
//
LOCUS       DADJYG010000344          808 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.356, whole genome shotgun sequence.
ACCESSION   DADJYG010000344 DADJYG010000000
VERSION     DADJYG010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.356"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..153
                     /locus_tag="JZ382_002907"
     CDS             <1..153
                     /locus_tag="JZ382_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAY2429015.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            215..>808
                     /locus_tag="JZ382_002908"
     CDS             215..>808
                     /locus_tag="JZ382_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429016.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRC"
BASE COUNT          287 a          121 c          158 g          242 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgag aaccttgggt tttcaagtgc tttttggttc
      241 attaatctta accatagtgt ttttaaagtg gaaaacaagc aacaaaaaaa gtatgtggag
      301 ccttgtagga atagttctta ttatttttac tgtgactgca gcaacagaag caaattattc
      361 gcataaaatt cagtcgtata aaggtcgttt agttatgacc aaaaaagatg ttacacgtta
      421 tacatcctca agaactggag gcctatatga ctatgcacca ttagatgcat tgaaatataa
      481 agatcattta aagaaacatg aagtaaaagt tggtgatcgg tgggagaatg catctcataa
      541 agcatataat tccaaaatag tctatacagt agaagatgcc gatgagcaaa atatcacatt
      601 acctgtattc aattattggg gaacagttgt gaaagtaaat gggaaaaaga gcactataga
      661 aaatgacaat ggtttagtgt ctattaaagg tacggggaaa agaactatag ttgaggtttc
      721 atctacgtat ccaactacga tgattctatc tttattaatt agttcaataa cttttatatt
      781 cttatttaca cgttatttaa taagatgc
//
LOCUS       DADJYG010000345          800 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.357, whole genome shotgun sequence.
ACCESSION   DADJYG010000345 DADJYG010000000
VERSION     DADJYG010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.357"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..258
                     /locus_tag="JZ382_002909"
     CDS             <1..258
                     /locus_tag="JZ382_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAY2429017.1"
                     /translation="NGWKVKLQERFELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKV
                     VDVKGMQTKDFKIKAKLFCHQYQAPLILAKKYRNTFKEERF"
     gene            273..434
                     /locus_tag="JZ382_002910"
     CDS             273..434
                     /locus_tag="JZ382_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAY2429018.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            431..736
                     /locus_tag="JZ382_002911"
     CDS             431..736
                     /locus_tag="JZ382_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429019.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
BASE COUNT          309 a          121 c          190 g          180 t
ORIGIN      
        1 aatggctgga aagtaaaact tcaggaaaga tttgagctga tgccgaaatt tgaactagac
       61 ggaaagaagt atcgcaagat cgagtatatt cccgacttca cattttataa aaacggcaaa
      121 cttgtcaaag tcgtagatgt caaaggaatg cagacaaaag actttaagat caaggcaaag
      181 ttgttctgtc atcaatatca agcgccgttg atattagcta aaaaatatcg gaatacgttc
      241 aaggaagagc gtttttaacg aggtggtcca tcatgacaac agaagaagtg attcaaatgc
      301 gtattcgaaa cattcagcgt gaaattgacg atctagaacg aacaaaggca gtgatggtca
      361 atgaaacggc gagaaaggca atcgatttgc acatagagaa cttaagaagg gaaattcgta
      421 gattggagga atgagcgtgg ataagaaagc gacaatgaaa cgaattgctg aattaaccaa
      481 gtcagaatct tggcaggaag acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc
      541 aatgtggact gaaaagccta aacggaaaac gccgagaaaa attgcaatct ggcatggtga
      601 tcgaattcta gtaacaggta cagctgaaca gttatctgaa attactggat taagcaaaaa
      661 cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac agtttaggta
      721 tgtggaggag agataatgga tctcattaca caatacagtg acatcatcct caagaaaatc
      781 atgatgaaga ttcagaaaga
//
LOCUS       DADJYG010000346          791 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.358, whole genome shotgun sequence.
ACCESSION   DADJYG010000346 DADJYG010000000
VERSION     DADJYG010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.358"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..590
                     /locus_tag="JZ382_002912"
     CDS             <1..590
                     /locus_tag="JZ382_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAY2429020.1"
                     /translation="SNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQS
                     RDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKR
                     ITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGL
                     GFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          285 a           88 c          148 g          270 t
ORIGIN      
        1 tgagtaatgg aataattcca agtgttagtg gagatacgat tcggtctgaa aaaaattatt
       61 ctatcattgt ttttgaaaag ttagcacaaa cgtctataac attaggaatg gatataatag
      121 aagcgtatca aagtagagat gctttgatac aagaaaacga attagctgta tcgttacctg
      181 aggtcttaaa agtgagggat tctgggattg tttattatac taaggaaatc ggaaaaacta
      241 agatagagca tctttctccc ttaatttctt ctgtcgttca attcatcggc ttaaatatct
      301 ataaaagaat tactgtaaag gaaatagcta attatttttc tgtaagtgaa actaaattaa
      361 gagaaagctt taagaatgaa atgcatatta cgatttataa ttatatctct aagcgaaaaa
      421 tttctacggc taaaataatg ttgaaatcaa accatactat cagtgaagtt tctttaggtt
      481 taggattttc agattcttca cacttttcga gagtattcaa aaagtacgca ggtgtatctc
      541 ctaagcaata tcaattaggt ttagtggaca atttcaacaa cattagctga gaggttttat
      601 tttattatga gactatatca tttacgtagt ttggaggagt tgaggaaatt ggattttaac
      661 aaaaaatttt tttgggaaat ttctgatttg agttgtttcg tagaatctga gcgtctaagt
      721 aatggtaata atagtgtgtt aaatgaatat gacaaagtag taattaataa agtaaaaagt
      781 ttatgtgaag a
//
LOCUS       DADJYG010000347          783 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.359, whole genome shotgun sequence.
ACCESSION   DADJYG010000347 DADJYG010000000
VERSION     DADJYG010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.359"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>783
                     /locus_tag="JZ382_002913"
     CDS             <1..>783
                     /locus_tag="JZ382_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY2429021.1"
                     /translation="KKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGNNLANIFSI
                     KNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYY
                     DRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLS
                     NSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFSISNALR
                     TAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTAR"
BASE COUNT          296 a          100 c          139 g          248 t
ORIGIN      
        1 taaaaagatt agttgtgtaa aaaaagatgg tacaagattc tctcaagaat atgacaaagt
       61 tatattagct acaggttcaa agcaaattat gctagatata ccggggaata atttagctaa
      121 tattttttcg ataaagaatt ttccaaatgc aaaagagatt tttaaagctt tggatgatag
      181 tactattaaa aaagtaggaa ttattggagc aggatatatt ggagttgaaa ttgctgaagc
      241 cataagaaaa agagggaaag aagtatatct tttcgatgtt gcagatagag tgttgtcgac
      301 ttattacgac cgtagttttt cagataaagt tgaagaaata ctatcaaaaa atggaataca
      361 tctttgtcta tcagaaagtc ctaaaaaata tttaggaaga aaaaagattg aaaaaatagt
      421 cactacaagt ggtacccaat actctatgga tatggtcgta caagctatag gatttctatc
      481 taatagtcca attggggaaa aggaactctt gagagatgtt agtggagcat atctaactaa
      541 tgaataccag caaacaaata taaaagatat atatgctatt ggggattgtg ctacaacttt
      601 ttttacgtcc attaatagaa gtagtgttga tttttctatt tctaatgctc taagaacagc
      661 ttatattgca actcaacata ttaacaatca agattttaca ccttctggtt cccaatttac
      721 aaatggcttt tctatattta attacaattt ttacgcagca ggtttgaatt taaaaacagc
      781 acg
//
LOCUS       DADJYG010000348          777 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.360, whole genome shotgun sequence.
ACCESSION   DADJYG010000348 DADJYG010000000
VERSION     DADJYG010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.360"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>777
                     /locus_tag="JZ382_002914"
     CDS             <1..>777
                     /locus_tag="JZ382_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAY2429022.1"
                     /translation="LFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYL
                     ILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFD
                     KKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIW
                     MSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYY
                     SGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTV"
BASE COUNT          234 a           94 c          136 g          313 t
ORIGIN      
        1 ttttgttttc aaatgctttt tttgatatga accaacttgt atttaatttg atgttattac
       61 aaagttttgc tagttttgta attagttata tccagtctta ttttacgatt aactttaacc
      121 attttaaata tttgatttta tcttttatat ctactttttt gaatattgca atctctgtag
      181 ctttgatgca gacattatat tttgatgata aatatgttgg cagagtagtc ggcggaacac
      241 tgtcatttgt gttgataggt ttagtaattt atttaagaat aatgataaag ggtgctcatt
      301 tttttgataa aaaacagtgg agatacgctt taaaaatctc tttgccgcta atcccacatt
      361 ccttagcaaa tatagtttta gcacaattcg atagaattat gattaattct tatttaggtc
      421 ctgctgcggg gggaatatat tcctacatta gtaacatagg tattattcta agcgtaatat
      481 ggatgtctac taataacgcc tgggttccat ggttctatgg tgagatgagt aaaaataatg
      541 aaaaaaaagt gaagaaagtt agtaactatt atttagtagt ctttactttg attacgatga
      601 ttgtaatggt agtttcgatt gatttagcaa aatttatggc tccgaaagac tattattctg
      661 ggttatcttt agtggttccg ataaccttag gctatttctt tcagttttta tatagtttac
      721 cagttaatgc tgaattttat gagaaaaaaa catcgtttat tgcgattggt acagtag
//
LOCUS       DADJYG010000349          772 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.361, whole genome shotgun sequence.
ACCESSION   DADJYG010000349 DADJYG010000000
VERSION     DADJYG010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.361"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          258 a          130 c          101 g          283 t
ORIGIN      
        1 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
       61 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      121 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      181 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      241 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      301 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      361 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      421 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      481 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      541 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      601 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      661 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      721 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gt
//
LOCUS       DADJYG010000350          756 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.362, whole genome shotgun sequence.
ACCESSION   DADJYG010000350 DADJYG010000000
VERSION     DADJYG010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.362"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..704
                     /locus_tag="JZ382_002915"
     CDS             <1..704
                     /locus_tag="JZ382_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAY2429023.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          255 a          123 c          145 g          233 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctacca
//
LOCUS       DADJYG010000351          752 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.363, whole genome shotgun sequence.
ACCESSION   DADJYG010000351 DADJYG010000000
VERSION     DADJYG010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.363"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(91..>752)
                     /locus_tag="JZ382_002916"
     CDS             complement(91..>752)
                     /locus_tag="JZ382_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAY2429024.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DADJYG010000352          731 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.364, whole genome shotgun sequence.
ACCESSION   DADJYG010000352 DADJYG010000000
VERSION     DADJYG010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.364"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>731
                     /locus_tag="JZ382_002917"
     CDS             <1..>731
                     /locus_tag="JZ382_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAY2429025.1"
                     /translation="SDAIIVIWIRLLVLAGKTNDDGLIYIQRNMPYTEEMLATLFGKN
                     VNTVRLALTTLANFNMIDLSSDGLIAISNWEKHQNIEGMDKVRLKNAERNRKYRERKR
                     QERLKLENDVSVTSRDGTDKDIEEDKDIDKEEKKGKYSDEHLRLAKKMQRNLTEDFPK
                     EMNKVDIEKWADTIRLMEERDKASIEAIEYVINWLPTNEFWFGNIRSAKKLREKFEKL
                     KFEIKADKKNHKKQSQKLQYSNPSE"
BASE COUNT          305 a          101 c          157 g          168 t
ORIGIN      
        1 tcggatgcca tcatcgttat atggattcga ttactagttt tagcaggaaa gactaacgac
       61 gatggtctga tatatatcca gaggaacatg ccttataccg aagaaatgct tgctacattg
      121 tttggcaaaa acgtaaatac ggttcgctta gcgctaacta cattggcaaa tttcaacatg
      181 attgatctaa gcagtgatgg actaattgcc atcagtaatt gggaaaaaca tcaaaatatc
      241 gagggtatgg ataaagtaag gctaaaaaat gctgaaagaa accgtaaata cagggaaaga
      301 aagagacagg aacgtctcaa attggaaaat gacgttagcg tgacgtcacg tgacggtaca
      361 gataaagata tagaagaaga taaagatata gataaagaag aaaagaaagg taagtattct
      421 gacgaacact tacgccttgc taaaaaaatg caaaggaatt taactgaaga ttttccaaaa
      481 gaaatgaaca aagtagatat cgaaaaatgg gcagacacaa tcaggttgat ggaagaaaga
      541 gataaagcat ctatagaagc gattgagtat gtgatcaatt ggctacctac aaatgaattt
      601 tggtttggaa atattagaag tgctaagaaa ttgagagaaa aatttgagaa gctcaaattc
      661 gaaatcaaag cagacaagaa gaatcataaa aagcaaagtc aaaaactaca gtacagcaat
      721 cctagtgaat a
//
LOCUS       DADJYG010000353          730 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.365, whole genome shotgun sequence.
ACCESSION   DADJYG010000353 DADJYG010000000
VERSION     DADJYG010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.365"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..717)
                     /locus_tag="JZ382_002918"
     CDS             complement(<1..717)
                     /locus_tag="JZ382_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2429026.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKV"
BASE COUNT          249 a           83 c          136 g          262 t
ORIGIN      
        1 cacttttcta aaatgttttt ttaagctggc tggactgata aaaaaaattt gggctaattg
       61 ttcaagcaaa aaattttctg aaagatgttc ttctaaatat cgtatgattg gatcaactaa
      121 ttcatctgaa taaagaatat gctttttttt aggaatgact ttctcatttg aaggtattgt
      181 caataaagca agttcctgtt gataatgcct tggtaaggaa gacaattgaa ttttttgcag
      241 acaataagta actaataagg aaagcaaatg aatgctcatc tgttggcata gtatgaaaaa
      301 agaattgatt tcctgatcat aggaaatagc tggcttacta tgttttagat aatcaatttt
      361 gaactgttca tttttcagaa aagtaatgat agatgttatt tcttgatcta tagaaaaaga
      421 ttgtcccgta atatgtgcta ataattgctc atcaaaataa aactggataa tgataaaagt
      481 ttgtgtttcc ttttgagtag aaaagatttg gacttcttgc tgtttatcaa tgaataaaag
      541 ggaaggatta tctagttcta tagatttatg tgcaaattca gcatataaat tagcgctttc
      601 atttattagt agtcgaaaat agggagcagc agataaatga agatgggtat ctatggaata
      661 ggttagtgtt tttttgacaa gattggagga ttggttgttt gttaacaaaa gaaccacttt
      721 cctttctctt
//
LOCUS       DADJYG010000354          721 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.366, whole genome shotgun sequence.
ACCESSION   DADJYG010000354 DADJYG010000000
VERSION     DADJYG010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.366"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            235..>721
                     /locus_tag="JZ382_002919"
     CDS             235..>721
                     /locus_tag="JZ382_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAY2429027.1"
                     /translation="MYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILP
                     LILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEI
                     FLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSID
                     SE"
BASE COUNT          225 a          103 c          109 g          284 t
ORIGIN      
        1 ggcttttatg atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc
       61 tcccctatta attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca
      121 tggttaactc tatatccttt atttttattg atcaagttta ctaatttagc aataggctat
      181 aacctttttc tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat
      241 gaaattacca aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct
      301 tttaggagtg tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata
      361 cttcctctta ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt
      421 atgttggcga ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact
      481 atggtaatga ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc
      541 ttcttaatta ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt
      601 ataggtccta tgattgagca gacattgtat caagatctga atagacctta tgtggatata
      661 ttacaaaaaa gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa
      721 t
//
LOCUS       DADJYG010000355          687 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.367, whole genome shotgun sequence.
ACCESSION   DADJYG010000355 DADJYG010000000
VERSION     DADJYG010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.367"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..421)
                     /locus_tag="JZ382_002920"
     CDS             complement(<1..421)
                     /locus_tag="JZ382_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAY2429028.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKY"
BASE COUNT          206 a          119 c           95 g          267 t
ORIGIN      
        1 gatatttttt tagatttctt tttagtcttt gtaatacttt ataagccaca gaataactca
       61 cgtgatgctg tttagcaaaa ttttctactg agatatattt ttcctgaaag aaagccaata
      121 ccattttcat agataaagaa tccgttatat aacgagataa cagagctttg ctagaacatt
      181 ttccattaat ttccagacat atatccaatt cactgacggt tactcgcaaa tttttctcta
      241 gatcgaactt ttgtaaatca tgtttcagct tatcgatcgt ttttttcaag agaaatgtcg
      301 atagcgacaa ctcttcttga acattcgata ttgactctga taaatttgag cttgcttcta
      361 aatatttaag taatgaataa cttctgtgat cttctttgct taaaaaaaac tcataattca
      421 ttagtcatca gctctttcta cgtatattac tttccatgca ttcaccaact agctggggat
      481 aaaactgatt tcctggatcc gagtcactgt tgtcagcgat tcttgtttgt ataatataca
      541 tcgtcctgtc ttttttttaa cattttggag agcgttttat ttggcctttc tcgcgatcaa
      601 acctgttatt agaattatat catttactag tatgcatttt tattaataaa atggtatatt
      661 ttatattgtt ataaaaagag cccaacc
//
LOCUS       DADJYG010000356          681 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.368, whole genome shotgun sequence.
ACCESSION   DADJYG010000356 DADJYG010000000
VERSION     DADJYG010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.368"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            200..>681
                     /locus_tag="JZ382_002921"
     CDS             200..>681
                     /locus_tag="JZ382_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429029.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     "
BASE COUNT          280 a           78 c          127 g          196 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa g
//
LOCUS       DADJYG010000357          670 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.369, whole genome shotgun sequence.
ACCESSION   DADJYG010000357 DADJYG010000000
VERSION     DADJYG010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.369"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            93..>670
                     /locus_tag="JZ382_002922"
     CDS             93..>670
                     /locus_tag="JZ382_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY2429030.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILS"
BASE COUNT          266 a           63 c          144 g          197 t
ORIGIN      
        1 aatcttttaa aaagcaaaaa gatgataaag ttacttttta tgggatgttg gtagaaaaca
       61 aattattata aatattagag gtataaaaag ttatgaaaca caaggtaagt gtgatagtac
      121 caatttacaa tgttgaaaaa tatttaagaa aatgtgtgga tagtttattg agtcagacat
      181 taaaagagat agaaatcgta ttagttgatg atggttcgcc agatgcttcg ggggaaatag
      241 cagatgagta tcaaaaaaaa tattctaatg taaaaacaat acatagagaa aatggggggt
      301 taggaccggc aagaaataca ggaattgaga atgcgacagg agaatatgtg gcttttcttg
      361 attcagatga ttgggtagaa agtgatatgt atgaaaaatt atacttagtt gcgtccaaag
      421 aaaatgctga tatagtagtt agtggacatt gtgattttgc gaatgacaaa gctatggtga
      481 aaaagcaaca tcctttgagt gggaatgtat atgatacgcc taatagtatt aaaaatgtac
      541 gaaaaaattt atttggacat agtccaaaag atcaagaagt agaggccttc ccgatgtcag
      601 tatgtatgtc tttgtataat aaaaatctgc tagataaata tagactaaga tttgaaaaaa
      661 ttttatctga
//
LOCUS       DADJYG010000358          669 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.370, whole genome shotgun sequence.
ACCESSION   DADJYG010000358 DADJYG010000000
VERSION     DADJYG010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.370"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            45..>669
                     /locus_tag="JZ382_002923"
     CDS             45..>669
                     /locus_tag="JZ382_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAY2429031.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFA"
BASE COUNT          217 a          100 c          132 g          220 t
ORIGIN      
        1 gtggaaaaag taatcgatta caataagcat aaaggagaaa aaatatgggc aaaagtagca
       61 ggaaggacat tgtatttgaa aaatttggta tagttgcgac taagttgggg aatcagatcc
      121 atttaagaac tttgcgagat gcgtttgcta catttatgcc atttatgatg ttagcaggat
      181 ttgtgacact tattaattca gtgatcttag aaccaacagg attcatgggg gaaattgtcg
      241 atactagtac attgaccacg ttacaacaaa ttggtacttc aattgccaat ggtacattaa
      301 gcataactac attattagtt gtagcagcag tatcttatca tatgtgtgca agcagaaatt
      361 atacaaatca tattgcagcg gtattagttg ctatttcgac atttgttgta ttaacaccta
      421 tgcagatgat gtttactcct gaaaatgcgg ataaagctgt tgaagtgacg ggtgtgatac
      481 ctatatccta tacaggagct tcaggaatgt ttgtaggtat atttgttgga ttagcggcta
      541 cagaattatt tattaaactt tctaataata agaaactaca gattcagcta tccggaaata
      601 ttcctcccgc tgttttaaag tcttttaatg tattaatccc aatcattatt acgattacct
      661 cgtttgctt
//
LOCUS       DADJYG010000359          657 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.371, whole genome shotgun sequence.
ACCESSION   DADJYG010000359 DADJYG010000000
VERSION     DADJYG010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.371"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>657
                     /locus_tag="JZ382_002924"
     rRNA            <1..>657
                     /locus_tag="JZ382_002924"
                     /product="23S ribosomal RNA"
BASE COUNT          164 a          141 c          211 g          141 t
ORIGIN      
        1 agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg cccaaaggtt
       61 ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc ttgactgcga
      121 gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt ccgcatggaa
      181 gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct cccccaagag
      241 tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct ggggctgtag
      301 tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg gttcagaacg
      361 tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag gagctgtcct
      421 tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg ccaagggcat
      481 tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt gaagcccacc
      541 tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg atcaggtaga
      601 taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc gatcgag
//
LOCUS       DADJYG010000360          657 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.372, whole genome shotgun sequence.
ACCESSION   DADJYG010000360 DADJYG010000000
VERSION     DADJYG010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.372"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..644)
                     /locus_tag="JZ382_002925"
     CDS             complement(<1..644)
                     /locus_tag="JZ382_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2429032.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSR"
BASE COUNT          171 a          145 c           92 g          249 t
ORIGIN      
        1 tgcctactat ttgatacccc actacctaat tcagctccaa tatcgtcaaa aaccaccaaa
       61 tcagttgttt taatatcggc tataagcgat ccttcaattt cttttctcag ttcagtattg
      121 ttataagaaa actttatttg ctctaataat tcttgatagc ttataaaaag tattttcttg
      181 tcataatttg agcgctcaag tatttcccaa gctgtggcca ttgataagtg gctttttccg
      241 cttcctgatt tccctgatag aatgaaatgt gcaggatggt tcagcagaac ctcattcgta
      301 tagctttttg ctttttctaa agcgattttt gtttcttgat ccactacgtg ataattttcc
      361 attttgcatt taaacaaagt tttatctgtt aataccgaac cattttgaaa aaaactcaac
      421 gctcgtgctt ttaagctatc gttatatatc cgttcggtct gtatatcctc tttcacacgt
      481 aacgctttat aaccacaact catgcatgtt ggtttacaac gttctgaacc atccttattt
      541 ttagctcgcc aactatacaa aggttcgcta cattctggac attttccgct ttgcactaat
      601 actcttctta ttagtttctc catagcattt gctaggcttt ccatgtgatg catctcc
//
LOCUS       DADJYG010000361          656 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.373, whole genome shotgun sequence.
ACCESSION   DADJYG010000361 DADJYG010000000
VERSION     DADJYG010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.373"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..644)
                     /locus_tag="JZ382_002926"
     CDS             complement(<1..644)
                     /locus_tag="JZ382_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2429033.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSR"
BASE COUNT          171 a          148 c           93 g          244 t
ORIGIN      
        1 tgcctactat ttgatacccc gctacctaat tcagctccaa tatcgtcaaa aaccaccaaa
       61 tcagttgttt tgatatcggc tataagcgat ccttcaattt cttttctcag ttcagcattg
      121 ttataagaaa actttatttg ctctaataac tcttgatagc ttataaaaag tattttcttg
      181 tcataatttg agcgctcaag tatttcccaa gctgtcgcca ttgataagtg gctttttccg
      241 cttcctgatt tccctgatag aatgaaatgt gcaggatggt tcagtaggac atcatttaca
      301 tagcttttag ctctttctaa agcaattttc gtttcttggt ccactacgtg ataattctcc
      361 attttgcatt taaacaaagt tttatctgtt aataccgaac cattttgaaa aaaactcaat
      421 gctcgtgctt ttaagctgtc gttatatatc cgttcggtct gtatatcctc tttcacacgt
      481 aacgctttat aaccacaact catacatgtt ggtttacaac gttctgaacc atccttattt
      541 ttagctcgcc aactatacaa aggttcgcta cattctggac attttccgct ttgcactaat
      601 actcttctta ttagcttctc catagcattt gctaggcttt ccatgtgatg catctc
//
LOCUS       DADJYG010000362          649 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.374, whole genome shotgun sequence.
ACCESSION   DADJYG010000362 DADJYG010000000
VERSION     DADJYG010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.374"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>649
                     /locus_tag="JZ382_002927"
     rRNA            <1..>649
                     /locus_tag="JZ382_002927"
                     /product="23S ribosomal RNA"
BASE COUNT          186 a          139 c          192 g          132 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatg
//
LOCUS       DADJYG010000363          646 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.375, whole genome shotgun sequence.
ACCESSION   DADJYG010000363 DADJYG010000000
VERSION     DADJYG010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.375"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..198
                     /locus_tag="JZ382_002928"
     CDS             <1..198
                     /locus_tag="JZ382_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429034.1"
                     /translation="NGDLKQINAKLMLSDVAIKQENTALKEKADSLAQINSKMMLASL
                     QNSKDISEIKEQLNPASKGGE"
     gene            200..337
                     /locus_tag="JZ382_002929"
     CDS             200..337
                     /locus_tag="JZ382_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAY2429035.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            375..>646
                     /locus_tag="JZ382_002930"
     CDS             375..>646
                     /locus_tag="JZ382_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429036.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          245 a           93 c          127 g          181 t
ORIGIN      
        1 aacggcgatt taaaacagat caatgccaaa ctcatgctaa gcgatgtagc aattaaacag
       61 gaaaatactg cattgaaaga aaaagcggat agtttagcac aaatcaattc aaaaatgatg
      121 cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact aaatccagct
      181 tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt atgattgggg
      241 ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg aagaaaaagc
      301 agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt tattttgatt
      361 caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga tcaataaccg
      421 ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc tcgaagatta
      481 ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat ttagaatcga
      541 gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata
      601 cacatgcaga aaaattggta gaacacacgc aacaaattaa aacttt
//
LOCUS       DADJYG010000364          591 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.377, whole genome shotgun sequence.
ACCESSION   DADJYG010000364 DADJYG010000000
VERSION     DADJYG010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.377"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..338
                     /locus_tag="JZ382_002931"
     CDS             <1..338
                     /locus_tag="JZ382_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429037.1"
                     /translation="KKDIENQINGFIQKLENANSYLINKELNKAYDQWKEVYDELKLI
                     QNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDNIDNALAELRYYVM
                     QIKDVRITR"
BASE COUNT          258 a           64 c          118 g          151 t
ORIGIN      
        1 caaaaaaaga tattgaaaat caaattaatg gatttataca gaaattagaa aatgcaaatt
       61 cttatttgat aaataaggaa ctaaataaag cttatgacca gtggaaagaa gtatatgatg
      121 agttgaaatt gattcaaaat gaaacaaaat tagtacgaac ggagaaaaag aatgaaaata
      181 gtttcttttt tgatggatat gctatattga tgttggaaac tgtagcaaaa caaaatataa
      241 aagctcccaa aaaagaacta tctgacaata ttgataacgc attagccgag ttaaggtatt
      301 acgttatgca aataaaagat gttaggatca ctcgttgagt ggtcttttta ttttgcacaa
      361 aggaggtaac aacaatgtat agaccacaat acttagaaca gaagtatgaa gtaatcactg
      421 tgcaaaatgg taacggagag atagtacgaa agtatagaag accaataaag agcgatacat
      481 ataaacgaaa ggaaagcaat gaagttattc cattgtatgg caaaagaata gctaagcatt
      541 aaataagatt gtgaaaggag acgggacatg aacgaggaat tctatagatg g
//
LOCUS       DADJYG010000365          583 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.378, whole genome shotgun sequence.
ACCESSION   DADJYG010000365 DADJYG010000000
VERSION     DADJYG010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.378"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..360
                     /locus_tag="JZ382_002932"
     CDS             <1..360
                     /locus_tag="JZ382_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAY2429038.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
     gene            366..>583
                     /locus_tag="JZ382_002933"
     CDS             366..>583
                     /locus_tag="JZ382_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429039.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDI"
BASE COUNT          213 a           97 c          124 g          149 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tggtgatcga gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg
      541 tacagcaacg aaacttcatc tatgcgctga taggcgatat ttt
//
LOCUS       DADJYG010000366          574 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.379, whole genome shotgun sequence.
ACCESSION   DADJYG010000366 DADJYG010000000
VERSION     DADJYG010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.379"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            339..422
                     /locus_tag="JZ382_002934"
     tRNA            339..422
                     /locus_tag="JZ382_002934"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:373..375,aa:Leu,seq:cag)
BASE COUNT          236 a           79 c          121 g          138 t
ORIGIN      
        1 aaaacgtgaa ttacttcagc gtctagaatt ccagaaggat agtaatcaac agaagaaata
       61 atcaatatcc aaatgaaaga agatagctga atcgagaaaa agaggaaaaa agaaatgaag
      121 aaaaaaataa aatagttact aatcccagag tcgttcgttt aacattgaaa tcgatcatat
      181 ggataaaaca gattttttca ccaaccctca atagatgatg gagttgctcg gatgaattat
      241 ttgtgtagat ggcataaggg agggcgtaca gaaatgaaaa agaacaaaaa tatgttcttg
      301 acaaaaaaca catgtaatcc taaactaaaa gatgtgatgc ggctatggcg gaattggcag
      361 acgcgctagc ttcaggcgct agtgagggtg acctcgtgga agttcgagtc ttcttagccg
      421 cataatttgt ttttttataa agagaaggta cacctggagc gatgagaaag aaaataacat
      481 attatctaaa agaatatagg aaaaatttta aacaacggaa ccagattcaa ataaatcaaa
      541 aaaaatttgt gtgaaggtgg gacagtttaa aacc
//
LOCUS       DADJYG010000367          564 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.380, whole genome shotgun sequence.
ACCESSION   DADJYG010000367 DADJYG010000000
VERSION     DADJYG010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.380"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..558
                     /locus_tag="JZ382_002935"
     CDS             <1..558
                     /locus_tag="JZ382_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429040.1"
                     /translation="NIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFF
                     TSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPW
                     EAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIE
                     RLLKQKTMKNILKNVKIAESLDDIL"
BASE COUNT          224 a           68 c          107 g          165 t
ORIGIN      
        1 aatatatata caggaagaga gaatacaata caaattccat tagttacagc tgggtcaaga
       61 accgcattgc aactttcgga tatttttaac cctatgatag atatttataa agaaaaaaaa
      121 tatatatttt ttacgtctgt ttatgatttt gagggcggag aacctgtcgg agaatatgaa
      181 ttagtatgca aaatagcaga cttagtagga aaagataatc taattgttaa gacgcatcct
      241 agagatgtaa gaacaattta taaagataat ggatttatcg aggataaaaa ttcatcaata
      301 ccttgggagg cgattcaatt gtcaggcgat tttaacgata aaatatttat gacaattaat
      361 tcaggaagtg ttatttcagg tagtacaatg tctgcaaaac ctgtacgcac gtattatatg
      421 tataaattgt gtaacataga aggaaatccg tcttgcaaaa agaatgcgta tgatattgag
      481 cgattgctta agcaaaaaac gatgaaaaac attttaaaga atgtgaaaat agcagaaagc
      541 ttagatgata tactatgagc aaaa
//
LOCUS       DADJYG010000368          563 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.381, whole genome shotgun sequence.
ACCESSION   DADJYG010000368 DADJYG010000000
VERSION     DADJYG010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.381"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..435
                     /locus_tag="JZ382_002936"
     CDS             <1..435
                     /locus_tag="JZ382_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAY2429041.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          181 a          129 c          109 g          144 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttc
//
LOCUS       DADJYG010000369          559 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.382, whole genome shotgun sequence.
ACCESSION   DADJYG010000369 DADJYG010000000
VERSION     DADJYG010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.382"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            54..559
                     /locus_tag="JZ382_002937"
                     /pseudo
     CDS             54..559
                     /locus_tag="JZ382_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
BASE COUNT          197 a           91 c          113 g          158 t
ORIGIN      
        1 caaaaaatct attttagtgt aaaattgaga aaaaagacag agaggacaga gtaatgaatc
       61 attttaaagg caaacaattc aaaaaagacg tcattattgt cgctgttggt tactacctgc
      121 gttacaatct aagctatcgt gaagttcagg aattgttata tgatcgtgga ataaatgttt
      181 gtcatactac gatttatcgt tgggtataag agtacagcaa agtcctctat tatctttgga
      241 agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat atcaaaatta
      301 agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc ttagatatct
      361 ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga ctccataaac
      421 agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc tccgccttta
      481 gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg aagtatctta
      541 acaatttaat agaacaaga
//
LOCUS       DADJYG010000370          547 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.384, whole genome shotgun sequence.
ACCESSION   DADJYG010000370 DADJYG010000000
VERSION     DADJYG010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.384"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>547
                     /locus_tag="JZ382_002938"
     rRNA            <1..>547
                     /locus_tag="JZ382_002938"
                     /product="23S ribosomal RNA"
BASE COUNT          165 a          112 c          166 g          104 t
ORIGIN      
        1 acttggaaaa gtcagtcaaa gagggtaaaa accccgtaga cgaaatgtgg aagacaccta
       61 ggaggatcct gagtacggcg gaacacgaga aattccgtcg gaatccggga ggaccatctc
      121 ccaaggctaa atactcccta gtgaccgata gtgaaccagt accgtgaggg aaaggtgaaa
      181 agcaccccgg aaggggagtg aaatagaacc tgaaaccgtg tgcctacaac aagtcaaagc
      241 ccgttaatgg gtgatggcgt gccttttgta gaatgaaccg gcgagttacg attgcatgcg
      301 aggttaagtt gaagagacgg agccgcagcg aaagcgagtc tgaatagggc gtttgagtat
      361 gtagtcgtag acccgaaacc atgtgatcta cccatgtcca ggttgaaggt gcggtaaaac
      421 gcactggagg accgaaccca cgtacgttga aaagtgcggg gatgaggtgt gggtagcgga
      481 gaaattccaa acgaacttgg agatagctgg ttctctccga aatagcttta gggctagcct
      541 cggaatt
//
LOCUS       DADJYG010000371          535 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.385, whole genome shotgun sequence.
ACCESSION   DADJYG010000371 DADJYG010000000
VERSION     DADJYG010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.385"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            63..>535
                     /locus_tag="JZ382_002939"
     CDS             63..>535
                     /locus_tag="JZ382_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429042.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKE"
BASE COUNT          201 a           97 c           95 g          142 t
ORIGIN      
        1 tcatttttat gtaagtgaat tatatcttaa tttatctatc atcaatactt aggagtgaat
       61 ctatggaaaa gccattcgtc aagtcattga ctgctgtggc ggtaggtgca ggtgctattg
      121 cgatctgttt gtttggatac catatcaata atcagcggca acatcagcaa cggatcaatt
      181 atgcagaatc tgccatcacc aatcaaaaag acaccttgac ttctttatca aaagaagtag
      241 acaagctgta tagtacaaaa gaaaaaattt ttctaaaccc tgaaataaca gaagaaacaa
      301 tcacaagttt gtcaaataag ctatcctctg tcaaactttc agcagatgat tacgacatca
      361 aagaatcaga attgcctaaa gaagctgttg caatccaaga tgagaagaaa gcaatcgtga
      421 cacagttaga tagtgcagaa tcaaaactga agatccagac agctgtcaac gatttattta
      481 ctaaaaatgt atcaaactgg cagcaagctg tagacgatgt catcatcaaa gaaaa
//
LOCUS       DADJYG010000372          530 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.386, whole genome shotgun sequence.
ACCESSION   DADJYG010000372 DADJYG010000000
VERSION     DADJYG010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.386"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            29..214
                     /locus_tag="JZ382_002940"
     CDS             29..214
                     /locus_tag="JZ382_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2429043.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            226..474
                     /locus_tag="JZ382_002941"
     CDS             226..474
                     /locus_tag="JZ382_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429044.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT          207 a           74 c          105 g          144 t
ORIGIN      
        1 attatttttt tagaaaggag ctatttttat gtctgagaat ttagacttaa aaattcgagc
       61 ggagatgaga aaaagaagaa tgactttcaa agaactagct gcgcttatcg gtatttcagg
      121 agcttattta tcagatattc taaacggcaa tcgtgatgga aagaaagcac aacagcatat
      181 cgaaacagtg aaaaaaatat tggacatccg ataggggggt aggcgatgac aaaactaaaa
      241 aaacaagatt ttgtaaaaaa atacaattat tctccatcta cttatcaacg tcgaatgtcg
      301 gaactaaaaa atacagcaat tttctcagcg gcgtatgaac gggtcacagg acaagaagtt
      361 tggatcaata cagaattata cgataaattt ttgtctttca aatcctataa caggttacgc
      421 acaagaaagg taacacctaa agaatttatc gagaagcatt tagttgattt ataaaaaaca
      481 gaacattttg agaggtgaag gttaatgggt aaattcaaca gagcattggt
//
LOCUS       DADJYG010000373          524 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.387, whole genome shotgun sequence.
ACCESSION   DADJYG010000373 DADJYG010000000
VERSION     DADJYG010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.387"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(4..357)
                     /gene="tnpB"
                     /locus_tag="JZ382_002942"
     CDS             complement(4..357)
                     /gene="tnpB"
                     /locus_tag="JZ382_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY2429045.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          114 c           90 g          167 t
ORIGIN      
        1 agcttaaaaa actccttttt ttcctggcaa aatagctcga ggttgatcga tacttaatcc
       61 ttctaacaac catcgaacct gttggttcgt taaggttcga acttcgtctt tcgtccgagg
      121 ccattttaac ttgccgtttt ctatccgctt ataacagaga agaaaaccat caccgtccca
      181 atagagaagt ttgaaacggt cttgacggtt cccgcaaaat aaaaaaacgg catcttcgta
      241 aagctcgagt tgatactctt gttgaataag actggcaaga ccatcaattc cttttcgcaa
      301 atctgttttt ccgcacacaa tataaatatg tttggtttga agtaaatcat aaagcattgg
      361 acaacagttc tttcaaaata gcttgaagaa tataattgtt aataccgtta tagatcaaaa
      421 cttcagaacc attctctctt ttgatgcgca tcgctaactt ggcagccgcg ggtttacctt
      481 tttctggtgc actcttttcg tttcctaatt ggacaggaac aatc
//
LOCUS       DADJYG010000374          515 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.388, whole genome shotgun sequence.
ACCESSION   DADJYG010000374 DADJYG010000000
VERSION     DADJYG010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.388"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..220
                     /locus_tag="JZ382_002943"
     CDS             <1..220
                     /locus_tag="JZ382_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY2429046.1"
                     /translation="KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            221..>515
                     /locus_tag="JZ382_002944"
     CDS             221..>515
                     /locus_tag="JZ382_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAY2429047.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQL"
BASE COUNT          199 a           59 c           99 g          158 t
ORIGIN      
        1 aaaatttcaa ttgtttactt tagatgaatg tatcaatgtt gacttattaa ataataaaat
       61 aagttttgaa gaaaacattg ttaaagaaat gcctgaatct gctaatgata tgtatgagaa
      121 ggagttgaaa tatttctttg aaaacgtagt aacaggcaaa gaaaactgga atgatttata
      181 tcatgcaaac gaagtattga aaatagctga ggagaaataa atggaaattt tatttactat
      241 ttgtggtaga gctgggtcta aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa
      301 gcccctagtc catttcgctt tagcaatgat agatatctat caacaaaaac atcctgaaca
      361 taattgcacg acttgtttga atacggacag taatgagtta gtagaattga cgactaagtt
      421 aaatccgtct gtgaaaattg tagaacgtaa acctgaatta gctacagata ctgtagggaa
      481 aattgaagtg atacgtgatt cattcttaca actag
//
LOCUS       DADJYG010000375          506 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.389, whole genome shotgun sequence.
ACCESSION   DADJYG010000375 DADJYG010000000
VERSION     DADJYG010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.389"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            92..>506
                     /locus_tag="JZ382_002945"
     CDS             92..>506
                     /locus_tag="JZ382_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAY2429048.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          204 a           59 c          103 g          140 t
ORIGIN      
        1 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
       61 aagcacaaat agcggagctg aaagtaatct tctggatgaa ttaaaaagaa ctgagattca
      121 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
      181 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
      241 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
      301 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
      361 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
      421 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
      481 ggcttcatat cctttcttaa tgccag
//
LOCUS       DADJYG010000376          506 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.390, whole genome shotgun sequence.
ACCESSION   DADJYG010000376 DADJYG010000000
VERSION     DADJYG010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.390"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>506)
                     /locus_tag="JZ382_002946"
     CDS             complement(<1..>506)
                     /locus_tag="JZ382_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAY2429049.1"
                     /translation="AQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLL
                     VYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGR
                     FTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFL
                     QVLVSSGI"
BASE COUNT          174 a           94 c           64 g          174 t
ORIGIN      
        1 attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt
       61 tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta
      121 ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc
      181 gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt
      241 aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct
      301 acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca
      361 aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt
      421 agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact
      481 aaaaaagaac aaattactac ctgggc
//
LOCUS       DADJYG010000377          499 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.391, whole genome shotgun sequence.
ACCESSION   DADJYG010000377 DADJYG010000000
VERSION     DADJYG010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.391"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>499)
                     /locus_tag="JZ382_002947"
     rRNA            complement(<1..>499)
                     /locus_tag="JZ382_002947"
                     /product="16S ribosomal RNA"
BASE COUNT          100 a          163 c          107 g          129 t
ORIGIN      
        1 gcatttcacc gctacacatg gaattccact ctcctcttct gcactcaagt ctcccagttt
       61 ccaatgaccc tccccggttg agccgggggc tttcacatca gacttaagaa accgcctgcg
      121 ctcgctttac gcccaataaa tccggacaac gcttgccacc tacgtattac cgcggctgct
      181 ggcacgtagt tagccgtggc tttctggtta gataccgtca agggatgaac agttactctc
      241 atccttgttc ttctctaaca acagagtttt acgatccgaa aaccttcttc actcacgcgg
      301 cgttgctcgg tcagactttc gtccattgcc gaagattccc tactgctgcc tcccgtagga
      361 gtttgggccg tgtctcagtc ccaatgtggc cgatcaccct ctcaggtcgg ctatgcatcg
      421 tggccttggt gagccgttac ctcaccaact agctaatgca ccgcgggtcc atccatcagc
      481 gacacccgaa agcgccttt
//
LOCUS       DADJYG010000378          496 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.392, whole genome shotgun sequence.
ACCESSION   DADJYG010000378 DADJYG010000000
VERSION     DADJYG010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.392"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..387
                     /locus_tag="JZ382_002948"
     CDS             <1..387
                     /locus_tag="JZ382_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY2429050.1"
                     /translation="RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGK
                     CAITLEEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIAL
                     LKLNKVQLIRLNKYRVLAGNQELNLI"
BASE COUNT          193 a           78 c           89 g          136 t
ORIGIN      
        1 agaaaattta tccacaccaa tctaagtgtt gataaattac tgatgtacca gttaatgaaa
       61 ttatctttgg aaaatcgcac aatagaatac gcagacaatc gaatttcgct attttctgct
      121 caacatggaa aatgtgctat aactctggaa gaatttcaac aagctaatga aatacattgt
      181 catcatattg taccaactgg agctggtgga aatgacgatt ataaaaatct tattttggta
      241 aaagaggcgg tacaccgtct aatacacgct aaaacagaag aaacaataca aaaatatata
      301 gctctattga agctaaataa agtccagctc ataagactaa ataaatatag agtattagct
      361 ggcaatcaag aattaaactt gatataggtc tactacataa atacttttca atataaacac
      421 gatggaacgc ggagtgcggt gaaagtcgca cgctccgtgt gaatgggggg aaaagtttga
      481 gatatttata atatca
//
LOCUS       DADJYG010000379          493 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.393, whole genome shotgun sequence.
ACCESSION   DADJYG010000379 DADJYG010000000
VERSION     DADJYG010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.393"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..465
                     /locus_tag="JZ382_002949"
     CDS             <1..465
                     /locus_tag="JZ382_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429051.1"
                     /translation="EQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLK
                     KAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQ
                     EYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          155 a           79 c          100 g          159 t
ORIGIN      
        1 gaacaaggat tgtatggttg gaatgtgacg gactgtaaaa tctgttttaa gtatggctta
       61 tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat tgtattggaa
      121 caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt taaaatttat
      181 gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg tgcgaacatc
      241 gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc tgctcggtgt
      301 attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt ttgtttaaca
      361 gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg tcgtccaaat
      421 agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat tttatgttgt
      481 tatataaata tgg
//
LOCUS       DADJYG010000380          485 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.394, whole genome shotgun sequence.
ACCESSION   DADJYG010000380 DADJYG010000000
VERSION     DADJYG010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.394"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(62..259)
                     /locus_tag="JZ382_002950"
     CDS             complement(62..259)
                     /locus_tag="JZ382_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY2429052.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DADJYG010000381          485 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.395, whole genome shotgun sequence.
ACCESSION   DADJYG010000381 DADJYG010000000
VERSION     DADJYG010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.395"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          158 a           74 c           78 g          175 t
ORIGIN      
        1 ttaatttgac aattaagatt atattaaata ttatacagat aataacggaa tattatcatc
       61 gaaacagcta acaaaattgt cataaaaaac gagatggttg atattagaac ttgtgttttt
      121 gtttaggtct agagataaag agagacaatt tccaaatata tgtaatgaat acaaaaagac
      181 gtttgtcgcg gattaccgtt acaaacgtct tttattgatt ttccgagatc tcatcagtcg
      241 ccatttggaa tggttcactc cttatctaga ttcgtttaat gctcggactg cttgttcgat
      301 tgtaatacca aacgcaacgt tattttcgtt ttgtgcatca atcgccataa aacggttaga
      361 agttgtatca aaagctacgc ggtattgcat atttgttttt gtaaacgtca taaaattcat
      421 ctcctttgta atgtaattta gttcttgaaa gtttccttta aactttcgaa cttataccaa
      481 gttta
//
LOCUS       DADJYG010000382          472 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.396, whole genome shotgun sequence.
ACCESSION   DADJYG010000382 DADJYG010000000
VERSION     DADJYG010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.396"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>472)
                     /locus_tag="JZ382_002951"
     CDS             complement(<1..>472)
                     /locus_tag="JZ382_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAY2429053.1"
                     /translation="YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALT
                     FLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQ
                     KIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTH"
BASE COUNT          182 a           82 c           81 g          127 t
ORIGIN      
        1 atatgtgtca gcatatcact aggagccata ccacttaatt cttttcccag tgggactttc
       61 aattcttgtg ctctagattg ttgaataaaa ggaattaaga atcccgcact taaaaaaaag
      121 gtgaggacgg tagcttttaa aagacttaag agtctagcga ttttttggtc ccaaaaataa
      181 aaagaaagaa acaacgttcc tccaataaat acagaaacca tagcgacaga aagtaaatga
      241 gtatacacta ctagcgtcat gccaatagac aaccagtacc atttttggta atctctaata
      301 taaatttcgt aacaccccat tagtatcaaa ggcaaaaatg ttaaagcgac agcttctccc
      361 aatgaagcac gatggaatat gtcaatggca cgataggcgg aaaatgtata aataatcgaa
      421 aataacagcg aaatataacg attgtttttt atttgtttca tagaaaaata ag
//
LOCUS       DADJYG010000383          467 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.397, whole genome shotgun sequence.
ACCESSION   DADJYG010000383 DADJYG010000000
VERSION     DADJYG010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.397"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>467
                     /locus_tag="JZ382_002952"
     CDS             <1..>467
                     /locus_tag="JZ382_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY2429054.1"
                     /translation="TFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFF
                     VSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGI
                     VGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEV"
BASE COUNT          168 a           57 c           86 g          156 t
ORIGIN      
        1 acatttttga agagaaaaga aattcaatat attaagtcta aagtgacgca taataaaaaa
       61 atttcttatc aataccatgt aggacagtat ttacccgact ggcatccttg ggaagattat
      121 cgcaattttt ttgtttcaaa caaaaaaact aatgggtgta gagaaatatt cgctattgaa
      181 ttaccttgga ttatagatac atttgggaaa gttaaatcgt ttactgtaat gacccagaaa
      241 ttatccaatc ttgatattga ttattcggac agtttcactc ttattattga gcatgaaact
      301 ggtatagttg gaactttaag tgtagatgtt gtttcaagag tagctaaaag aaatttaact
      361 ataattggtg aaaagatttt aatagaatgg gaaggtaacc cggattctct attttcatgg
      421 aatgaagaaa cttctagtat gacagatatt caattgtacg atgaggt
//
LOCUS       DADJYG010000384          457 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.399, whole genome shotgun sequence.
ACCESSION   DADJYG010000384 DADJYG010000000
VERSION     DADJYG010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.399"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>457
                     /locus_tag="JZ382_002953"
     CDS             <1..>457
                     /locus_tag="JZ382_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429055.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          185 a           68 c          106 g           98 t
ORIGIN      
        1 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
       61 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DADJYG010000385          456 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.400, whole genome shotgun sequence.
ACCESSION   DADJYG010000385 DADJYG010000000
VERSION     DADJYG010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.400"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..395
                     /locus_tag="JZ382_002954"
     CDS             <1..395
                     /locus_tag="JZ382_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429056.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
BASE COUNT          181 a           71 c           86 g          118 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacg
//
LOCUS       DADJYG010000386          452 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.401, whole genome shotgun sequence.
ACCESSION   DADJYG010000386 DADJYG010000000
VERSION     DADJYG010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.401"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>452
                     /locus_tag="JZ382_002955"
     CDS             <1..>452
                     /locus_tag="JZ382_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429057.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DADJYG010000387          448 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.402, whole genome shotgun sequence.
ACCESSION   DADJYG010000387 DADJYG010000000
VERSION     DADJYG010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.402"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..302)
                     /locus_tag="JZ382_002956"
     CDS             complement(<1..302)
                     /locus_tag="JZ382_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAY2429058.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          140 a           79 c           78 g          151 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgac
//
LOCUS       DADJYG010000388          430 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.403, whole genome shotgun sequence.
ACCESSION   DADJYG010000388 DADJYG010000000
VERSION     DADJYG010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.403"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          188 a           60 c           70 g          112 t
ORIGIN      
        1 agcaataaaa tagtatttta aaaaacatat aaaatagttg ttgacatgtt agaaatgaca
       61 tgataagata actgagttgt taaaaaagaa ccacttgtga agcttgattc tatcgaaaaa
      121 aacttcaaaa aaagttcttg acaaataaca aatgataagt tatgataaat gagttgctgt
      181 aagatttcga aaaacgaaat caaaaaaact tttaaaaaag agttgacaat tacttgtctc
      241 tctgatatga tataaaagtt gctacggcag ccaaaaacga atcggggaac actcctgatg
      301 aagtagacct ttgaaaactg aacaaagtaa gacaaaccaa atgtgtaggg cgtcttgatt
      361 caattcaaga caacaaacat tttttaacaa gcaagcaata tgctagcaaa caaattgagc
      421 ttaacaatcg
//
LOCUS       DADJYG010000389          429 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.404, whole genome shotgun sequence.
ACCESSION   DADJYG010000389 DADJYG010000000
VERSION     DADJYG010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.404"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>429
                     /locus_tag="JZ382_002957"
     CDS             <1..>429
                     /locus_tag="JZ382_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2429059.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DADJYG010000390          423 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.405, whole genome shotgun sequence.
ACCESSION   DADJYG010000390 DADJYG010000000
VERSION     DADJYG010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.405"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            37..231
                     /locus_tag="JZ382_002958"
     CDS             37..231
                     /locus_tag="JZ382_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429060.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          183 a           55 c           78 g          107 t
ORIGIN      
        1 atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga agtaaaaaca
       61 aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc tttacttggt
      121 tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa gaacgagcta
      181 gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta aatccaatgt
      241 atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta tagaattaga
      301 agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc atatgattat
      361 ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga atcaaatata
      421 ctt
//
LOCUS       DADJYG010000391          415 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.406, whole genome shotgun sequence.
ACCESSION   DADJYG010000391 DADJYG010000000
VERSION     DADJYG010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.406"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..339)
                     /locus_tag="JZ382_002959"
     CDS             complement(<1..339)
                     /locus_tag="JZ382_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2429061.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DADJYG010000392          409 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.407, whole genome shotgun sequence.
ACCESSION   DADJYG010000392 DADJYG010000000
VERSION     DADJYG010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.407"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..379
                     /locus_tag="JZ382_002960"
     CDS             <1..379
                     /locus_tag="JZ382_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAY2429062.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DADJYG010000393          400 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.408, whole genome shotgun sequence.
ACCESSION   DADJYG010000393 DADJYG010000000
VERSION     DADJYG010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.408"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          111 a           98 c           76 g          115 t
ORIGIN      
        1 ttataaataa ctcataaaaa atattttgac tttcaaaatc acttttgcta ccagaagttg
       61 aggcgaagtg ttttaagcat agttgtaccc tagtttgacc gttaaaaata aataacgatc
      121 gctagagtgc aactatgttt gtttgcccta ccgtcagcgt cggcttggcc tctcacatcc
      181 attaccgtcg ggattatctc cgttacatac ccttacaaaa tacgccctgt aagcattgca
      241 tgaaaccacc gtgcatttgc accgtatttc cgccctagcc gttcaaatcg gtcgctacca
      301 ccattcagaa acacgtgcgg taatggaagg tttggaggaa gctatccaga ccactaacga
      361 ttgtccctat cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DADJYG010000394          385 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.409, whole genome shotgun sequence.
ACCESSION   DADJYG010000394 DADJYG010000000
VERSION     DADJYG010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.409"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(21..92)
                     /locus_tag="JZ382_002961"
     tRNA            complement(21..92)
                     /locus_tag="JZ382_002961"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Glu,seq:ttc)
     gene            complement(114..201)
                     /locus_tag="JZ382_002962"
     tRNA            complement(114..201)
                     /locus_tag="JZ382_002962"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Ser,seq:gct)
     gene            complement(226..299)
                     /locus_tag="JZ382_002963"
     tRNA            complement(226..299)
                     /locus_tag="JZ382_002963"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(263..265),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="JZ382_002964"
     tRNA            complement(312..382)
                     /locus_tag="JZ382_002964"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
BASE COUNT          112 a          101 c           89 g           83 t
ORIGIN      
        1 taaaattata ttgaatttaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
       61 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaaa caaaatattg aaacggagaa
      121 ggagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
      181 cagccacttg agtacttccc caaaaccaat aagattttgt actaatgggc ctaaatggac
      241 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
      301 taaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatg
//
LOCUS       DADJYG010000395          368 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.411, whole genome shotgun sequence.
ACCESSION   DADJYG010000395 DADJYG010000000
VERSION     DADJYG010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.411"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>368
                     /locus_tag="JZ382_002965"
     rRNA            <1..>368
                     /locus_tag="JZ382_002965"
                     /product="16S ribosomal RNA"
BASE COUNT           88 a           87 c          113 g           80 t
ORIGIN      
        1 tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta
       61 gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag
      121 ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt
      181 gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct
      241 taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag
      301 tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca
      361 acgagcgc
//
LOCUS       DADJYG010000396          363 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.412, whole genome shotgun sequence.
ACCESSION   DADJYG010000396 DADJYG010000000
VERSION     DADJYG010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.412"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(130..285)
                     /locus_tag="JZ382_002966"
                     /pseudo
     CDS             complement(130..285)
                     /locus_tag="JZ382_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
BASE COUNT          125 a           69 c           39 g          130 t
ORIGIN      
        1 cattattgtt aaacaagaat acaagcaaga aagaactata gttaatatga taaaaattat
       61 taaagttcta tttttctttc ttgtaatata tgctatagca ttttttatca ctttcctaac
      121 tactcctctc tattttctta ctccataact aattgcatcc actggacaag catttttaca
      181 tcgtccacat ctgatacact ccagatgatt gcaattctca actgaatctt tattcatttg
      241 gcaaactttt ttacattttc cacaattaat acacttatcc ttatcctgaa tcggctacac
      301 tagactagac agaaaaaata aggtgtgtag aataataaaa aacacactgg aggatttttc
      361 atg
//
LOCUS       DADJYG010000397          340 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.413, whole genome shotgun sequence.
ACCESSION   DADJYG010000397 DADJYG010000000
VERSION     DADJYG010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.413"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT           90 a           77 c           42 g          131 t
ORIGIN      
        1 ccatctatag aattcctcgg tcatgtccca tctcctttcg caatcttatt taatacttag
       61 ctattctttt gccatacaat ggaataactt cattgctttc cttccgttta tatgtatcgc
      121 tctttattgg tcttctatac tttcgtacta tctcaccgtt accgttttgc acagtgatta
      181 cttcatgctt ctgttctaag tattgtggtc tatacatcgt cgtgcctcct ttacgcaaaa
      241 taaaaagacc actcaaagag tgatctacga attttaaaaa atctcatact gaaatcttat
      301 ttttaatgtc tttatataag ttcgagcctc gcaaaacata
//
LOCUS       DADJYG010000398          340 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.414, whole genome shotgun sequence.
ACCESSION   DADJYG010000398 DADJYG010000000
VERSION     DADJYG010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.414"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>340
                     /locus_tag="JZ382_002967"
     CDS             <1..>340
                     /locus_tag="JZ382_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429063.1"
                     /translation="LALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDT
                     GILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLN
                     GGRIAASLHSD"
BASE COUNT           98 a           40 c           70 g          132 t
ORIGIN      
        1 ttagctttaa gtggtgcaat tctagtgtat tatgttttat taaagtgggg aaaaaagatt
       61 caaatccgtg cattagtgtg tactatttgt gcaactgtta tttcgttagt gtttatttac
      121 attatagata caggaatttt ggcattgctt atgtcaaatt tgggcattac agatgacggt
      181 gcgaggcttt cattttatct atattcatct aaattttatg aacttggttt aggatatgta
      241 ggaacaggat ggacatggtt ttcaaaatac ttttttaatc tttatttatc gcactttagg
      301 ttaaatggtg gaagaattgc agcaagccta cacagtgata
//
LOCUS       DADJYG010000399          333 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.415, whole genome shotgun sequence.
ACCESSION   DADJYG010000399 DADJYG010000000
VERSION     DADJYG010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.415"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          113 a           56 c           55 g          109 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtcatgaa tcggctacac tagactagac agaaaaaata aggtgtgtag
      301 aataataaaa aacacactgg aggatttttc atg
//
LOCUS       DADJYG010000400          329 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.416, whole genome shotgun sequence.
ACCESSION   DADJYG010000400 DADJYG010000000
VERSION     DADJYG010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.416"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          122 a           58 c           55 g           94 t
ORIGIN      
        1 aataggaaaa aaactatttt tgatcattga caattaaata caatttttga ataacgatcc
       61 ctgctattcg agctagaaac tcaagtacgc catgacctga ttagcgacaa atacttagag
      121 atttcttttt gttctgggaa aacaaaatgc catgacaata gcagcaaata atgatacttc
      181 cgtaaggctc gccgtaaact ggggagaggt gaaattccta tgtgcttacc caaagcaatt
      241 caaaaatttc ataactacta ttttaggaga tgattttata gaatccagag tctacctgaa
      301 taaaaaacaa acctgtgcat ttctaggaa
//
LOCUS       DADJYG010000401          325 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.417, whole genome shotgun sequence.
ACCESSION   DADJYG010000401 DADJYG010000000
VERSION     DADJYG010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.417"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            63..>325
                     /locus_tag="JZ382_002968"
     CDS             63..>325
                     /locus_tag="JZ382_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429064.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYV"
BASE COUNT          149 a           47 c           50 g           79 t
ORIGIN      
        1 gttttcatat aaccaaaggc gctatgattt ccatatgaaa aaaataaagt caggtgaaaa
       61 aaatgaaact tgaggataaa aaagtcattt tgaagaaaat caaacgacta ccagaccaaa
      121 tagatggcta tacgatctgt tctttcaaaa aagacaggag tataaaagct tatacgcaaa
      181 acacagaaaa aggaataatc tatttcctac atgaagaagg ttacgatacc caaaccttta
      241 tgtttactga aaaaaaagaa tttcataaac agatgaaaaa actgattgaa aaagaatttc
      301 caagaagtca taaactctat gtgaa
//
LOCUS       DADJYG010000402          321 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.418, whole genome shotgun sequence.
ACCESSION   DADJYG010000402 DADJYG010000000
VERSION     DADJYG010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.418"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>321)
                     /locus_tag="JZ382_002969"
     CDS             complement(<1..>321)
                     /locus_tag="JZ382_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY2429065.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DADJYG010000403          311 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.419, whole genome shotgun sequence.
ACCESSION   DADJYG010000403 DADJYG010000000
VERSION     DADJYG010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.419"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..270
                     /locus_tag="JZ382_002970"
     CDS             <1..270
                     /locus_tag="JZ382_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429066.1"
                     /translation="NLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          138 a           42 c           36 g           95 t
ORIGIN      
        1 aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga gaacaatcat
       61 aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat ttacaaagca
      121 acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac ttttagtttg
      181 gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga agggacagct
      241 aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta caaaacaata
      301 atttcaccga a
//
LOCUS       DADJYG010000404          299 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.421, whole genome shotgun sequence.
ACCESSION   DADJYG010000404 DADJYG010000000
VERSION     DADJYG010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.421"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            1..299
                     /locus_tag="JZ382_002971"
                     /pseudo
     CDS             1..299
                     /locus_tag="JZ382_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          119 a           59 c           56 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcag
//
LOCUS       DADJYG010000405          293 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.422, whole genome shotgun sequence.
ACCESSION   DADJYG010000405 DADJYG010000000
VERSION     DADJYG010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.422"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>293
                     /locus_tag="JZ382_002972"
     CDS             <1..>293
                     /locus_tag="JZ382_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAY2429067.1"
                     /translation="MHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLS
                     EVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPT"
BASE COUNT          110 a           50 c           49 g           84 t
ORIGIN      
        1 atgcattata cctatcctaa tttaaacaac agtcaaattg aatcaaaaag tcttaatcaa
       61 gatcaaataa taaaatatgc aaattactgt ggtgtttatc ctaaaaaagt agcacgaata
      121 ctaaaccttt cagaggtcca aacacaacta agaaaaggaa atgccattta tatgggaact
      181 gctggttatg gaaattatag taacagccgt catgcacttg cattatttgg gtgggtcaat
      241 caaggtggtt atcaaactta ctatgtgtgg aatccttggt ataactaccc aac
//
LOCUS       DADJYG010000406          291 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.423, whole genome shotgun sequence.
ACCESSION   DADJYG010000406 DADJYG010000000
VERSION     DADJYG010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.423"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>291
                     /locus_tag="JZ382_002973"
     CDS             <1..>291
                     /locus_tag="JZ382_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAY2429068.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           86 a           55 c           58 g           92 t
ORIGIN      
        1 agctcgtcaa acaatcgaag agactttttc ctgaaatcaa ttgtgttgtc gctgatgctg
       61 ggtataagac acctaagttt gttcactttt tgactcattt gaaccttcga ccttgtttgc
      121 cttattcaag gcccaaaggg aaaaaggggc tattatcaaa aaatgagttt ctctatgacg
      181 agtattttga ttgttatatt tgtccgcaag atcaaatgct cgctttttca acggttactc
      241 gagaagggta ccgagaatac aaatccaatc caaaagaatg tgtgaactgt c
//
LOCUS       DADJYG010000407          286 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.424, whole genome shotgun sequence.
ACCESSION   DADJYG010000407 DADJYG010000000
VERSION     DADJYG010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.424"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
BASE COUNT          106 a           31 c           53 g           96 t
ORIGIN      
        1 tctattagga aatataagaa ggagtataaa aatgaacaaa cataatagaa aaataacgaa
       61 taacaaagaa ctgatcttgt tactaatagg gatttggggt atctttttac tctttaatat
      121 gtatatgccg actatgagag cggatgacgt agtttatgca agtagattag atgaacttgg
      181 ctatttgggt gcttcgatag aacattataa gacatggagt tcaagaatta ttatagaact
      241 attcttaatg ttcttttcta aacactttat gctatggaaa ttatta
//
LOCUS       DADJYG010000408          273 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.425, whole genome shotgun sequence.
ACCESSION   DADJYG010000408 DADJYG010000000
VERSION     DADJYG010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.425"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(<1..>273)
                     /locus_tag="JZ382_002974"
     CDS             complement(<1..>273)
                     /locus_tag="JZ382_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429069.1"
                     /translation="LEDHDSFVFDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPK
                     QQFKAATDFILKNEDKTERVENLHFETEKYKDHPVDYQPLTEKGRN"
BASE COUNT           73 a           45 c           45 g          110 t
ORIGIN      
        1 taattccttc ctttttctgt gagtggttga taatcaacgg ggtgatcttt gtacttttct
       61 gtctcaaaat gaagattttc cactcgttct gttttgtctt cattctttag aataaaatca
      121 gtggctgctt taaattgttg cttagggtct tggatctctt tgtctatgaa aaaaagttta
      181 gctaattcaa taatgtcgcc actaatattt tgactgttcc aataaaaacg ttgttttctt
      241 cgatcaaaaa caaatgaatc atggtcttct aag
//
LOCUS       DADJYG010000409          270 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.426, whole genome shotgun sequence.
ACCESSION   DADJYG010000409 DADJYG010000000
VERSION     DADJYG010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.426"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..>270
                     /locus_tag="JZ382_002975"
     CDS             <1..>270
                     /locus_tag="JZ382_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAY2429070.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DADJYG010000410          262 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.427, whole genome shotgun sequence.
ACCESSION   DADJYG010000410 DADJYG010000000
VERSION     DADJYG010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.427"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            <1..186
                     /locus_tag="JZ382_002976"
     CDS             <1..186
                     /locus_tag="JZ382_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY2429071.1"
                     /translation="TLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALK
                     FQQTADKRIKPVKGSIA"
     gene            183..>262
                     /locus_tag="JZ382_002977"
     CDS             183..>262
                     /locus_tag="JZ382_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAY2429072.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDL"
BASE COUNT          101 a           49 c           52 g           60 t
ORIGIN      
        1 acgctcttag aagccagaca gaacaaggca acgatcatca caacaaactt atctggtcct
       61 gaactaagag aagcctacgg tgaaagaatt gtttctagga tatttaagaa ttcagaaggt
      121 tatgcgctga aattccaaca aacagcagac aagcgcataa aaccagtgaa aggtagtatc
      181 gcatgaataa ataccgtaat aaaaaaactg ttcatcgagg tatcaagttt gattctatcg
      241 cagaagcaga gtattacgat ct
//
LOCUS       DADJYG010000411          260 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.denovo.428, whole genome shotgun sequence.
ACCESSION   DADJYG010000411 DADJYG010000000
VERSION     DADJYG010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.denovo.428"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            100..>260
                     /locus_tag="JZ382_002978"
     CDS             100..>260
                     /locus_tag="JZ382_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAY2429073.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          100 a           48 c           39 g           73 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata
//
LOCUS       DADJYG010000412        14828 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.063, whole genome shotgun sequence.
ACCESSION   DADJYG010000412 DADJYG010000000
VERSION     DADJYG010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.063"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            177..1073
                     /locus_tag="JZ382_002979"
     CDS             177..1073
                     /locus_tag="JZ382_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAY2429074.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1223..2164)
                     /locus_tag="JZ382_002980"
     CDS             complement(1223..2164)
                     /locus_tag="JZ382_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY2429075.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2290..3411
                     /locus_tag="JZ382_002981"
     CDS             2290..3411
                     /locus_tag="JZ382_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAY2429076.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3669..5171
                     /locus_tag="JZ382_002982"
     CDS             3669..5171
                     /locus_tag="JZ382_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAY2429077.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5238..5765
                     /locus_tag="JZ382_002983"
     CDS             5238..5765
                     /locus_tag="JZ382_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY2429078.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5976..7037
                     /locus_tag="JZ382_002984"
     CDS             5976..7037
                     /locus_tag="JZ382_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAY2429079.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7040..7936
                     /gene="murQ"
                     /locus_tag="JZ382_002985"
     CDS             7040..7936
                     /gene="murQ"
                     /locus_tag="JZ382_002985"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY2429080.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7950..9932
                     /locus_tag="JZ382_002986"
     CDS             7950..9932
                     /locus_tag="JZ382_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAY2429081.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9997..10443)
                     /gene="nrdI"
                     /locus_tag="JZ382_002987"
     CDS             complement(9997..10443)
                     /gene="nrdI"
                     /locus_tag="JZ382_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY2429082.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10654..12078
                     /locus_tag="JZ382_002988"
     CDS             10654..12078
                     /locus_tag="JZ382_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY2429083.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12259..13032)
                     /locus_tag="JZ382_002989"
     CDS             complement(12259..13032)
                     /locus_tag="JZ382_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY2429084.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13029..13934)
                     /locus_tag="JZ382_002990"
     CDS             complement(13029..13934)
                     /locus_tag="JZ382_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY2429085.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(14088..14408)
                     /locus_tag="JZ382_002991"
     CDS             complement(14088..14408)
                     /locus_tag="JZ382_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAY2429086.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4760 a         2629 c         3118 g         4321 t
ORIGIN      
        1 caattacaac tattcagaaa acttttaggc gaattttaat agaagaatga tttatttcat
       61 catattttca tacaaatgtt aaattttcat acaaatatag gaaacgtttt actggctttt
      121 tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg gaaaagatga
      181 acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg ccttccatcc
      241 gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt aaatcagcta
      301 gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt gatatggaga
      361 tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt tggaatcact
      421 tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca atcgatgaga
      481 tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat agttcatttt
      541 attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt tttggtgcaa
      601 cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata aaaagagatg
      661 ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga tatcttactt
      721 atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg ttgcgggagg
      781 gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag aaactagtga
      841 cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt tttttgcttc
      901 gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag ccaatcctat
      961 cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc actaacgatt
     1021 ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa taaaaaacct
     1081 acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc atcatgagag
     1141 ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct gctgattcag
     1201 ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt gcatggacta
     1261 attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct tttcgccaaa
     1321 tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg tcttggtaca
     1381 tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata tgttggtaca
     1441 cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat tcagatacga
     1501 ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata ttacaatcca
     1561 aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa atggaataac
     1621 gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct acaacttttt
     1681 ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca tcacctaaca
     1741 atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca aataagatat
     1801 ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt agtaaacaca
     1861 aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca tctaattgat
     1921 tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg atttcttctg
     1981 gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca atcaacccat
     2041 ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc tcatttccgc
     2101 tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct attgcgattt
     2161 tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa aaatggaaga
     2221 aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag aagaaagtga
     2281 gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc ttcttcattt
     2341 gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa aatcaatgtg
     2401 ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct caggggaaca
     2461 tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag ctatttattg
     2521 acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt ggggttagat
     2581 ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc cttaggagaa
     2641 cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt aggaggaagc
     2701 gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt agaagggtac
     2761 acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag aaacgttctt
     2821 gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac aggtcaacaa
     2881 ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct agctttctta
     2941 gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt ggatttagat
     3001 gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt gttgggagcg
     3061 aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa aaagatcgaa
     3121 caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat ccacggaaaa
     3181 gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat cgctatttgc
     3241 ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt cttaagtatt
     3301 catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt agaaaaaaca
     3361 aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta gttgacaaaa
     3421 tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat ccgtgcattg
     3481 aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag acaaggaagc
     3541 agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt ttgtgtgtat
     3601 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     3661 gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac agccaaggca
     3721 cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta ggacttatcg
     3781 gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa ctaccttatc
     3841 aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca aaagataaag
     3901 aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg gaaatcgaaa
     3961 gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc agcacactat
     4021 cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac actcatttcc
     4081 cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa gtagcggctt
     4141 atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga tttatcgatg
     4201 aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat caagggaatt
     4261 tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct caaaatgaaa
     4321 aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc gaatggtctc
     4381 gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact gaatctagac
     4441 gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga tccaaagcaa
     4501 tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa gatatcgaat
     4561 atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt ttaagcgtag
     4621 aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt acaatcgagc
     4681 ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc attatccatt
     4741 atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca aaacatctga
     4801 gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg gaatttgcag
     4861 agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt gggatggaaa
     4921 gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa aaagtggaat
     4981 tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca ttgaattatt
     5041 tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa cctgccatgt
     5101 tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt atttctcttg
     5161 agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg aactttgcca
     5221 acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact tggaacgcac
     5281 aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag atgcaggcac
     5341 agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc atccattccc
     5401 ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc ttgccgctga
     5461 ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg ccaagcaaag
     5521 atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat ggggagtcag
     5581 tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa gacgaagaaa
     5641 atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct tattttcatc
     5701 cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg gtttcttatg
     5761 agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt ttgcttagca
     5821 gtgccttttt taggttatga caattctttt gataatataa actcaacctt tagaaatgaa
     5881 atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat
     5941 agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga
     6001 acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg
     6061 tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat
     6121 tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag
     6181 gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg
     6241 gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac
     6301 atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga
     6361 aagaagcaga agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg
     6421 aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc
     6481 tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat
     6541 tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag
     6601 caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc
     6661 aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag
     6721 aattttacga atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca
     6781 tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta
     6841 cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga
     6901 tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta
     6961 tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa
     7021 gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag
     7081 aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa
     7141 caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt
     7201 gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg
     7261 aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga
     7321 accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga
     7381 aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa
     7441 aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt
     7501 agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga
     7561 aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg
     7621 ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat
     7681 cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa
     7741 tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga
     7801 aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat
     7861 tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt
     7921 aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac
     7981 tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact
     8041 gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa
     8101 aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac
     8161 ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag
     8221 aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg
     8281 taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa
     8341 tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg
     8401 gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac
     8461 ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt
     8521 ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa
     8581 ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac
     8641 tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca
     8701 tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg
     8761 cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt
     8821 tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg
     8881 tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg
     8941 ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg
     9001 gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat
     9061 tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt
     9121 taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag
     9181 gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga
     9241 tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct
     9301 ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg
     9361 ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg
     9421 aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg
     9481 tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc
     9541 cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata
     9601 tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga
     9661 tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag
     9721 gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg
     9781 tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct
     9841 tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag
     9901 caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa
     9961 gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat
    10021 ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg
    10081 aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt
    10141 atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc
    10201 acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa
    10261 ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg
    10321 gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata
    10381 ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt
    10441 catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg
    10501 tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt
    10561 atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt
    10621 gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga
    10681 attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt
    10741 ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc
    10801 gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca
    10861 gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt
    10921 atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg
    10981 ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg
    11041 atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa
    11101 aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt
    11161 attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac
    11221 attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc
    11281 atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca
    11341 aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt
    11401 tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc
    11461 aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc
    11521 gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta
    11581 ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa
    11641 ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc
    11701 tttttacttt cattattatt cactgacagt gcttataact ttgcttacac acttggtaca
    11761 gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag
    11821 cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct
    11881 atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt
    11941 tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat
    12001 aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact
    12061 gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt
    12121 tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca
    12181 gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc
    12241 ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa
    12301 gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa
    12361 ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt
    12421 ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga
    12481 agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc
    12541 aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca aagtataagt
    12601 gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg
    12661 gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga
    12721 ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata
    12781 aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc
    12841 aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg
    12901 atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa
    12961 tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac
    13021 aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat
    13081 tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc
    13141 tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct
    13201 tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag
    13261 ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc
    13321 tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga
    13381 ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct
    13441 cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg
    13501 ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct
    13561 tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca
    13621 tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc
    13681 gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca
    13741 ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg
    13801 atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag
    13861 ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc
    13921 gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg
    13981 agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata
    14041 ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta
    14101 gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga
    14161 ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct
    14221 gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa
    14281 aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca
    14341 ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc
    14401 catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc
    14461 ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat
    14521 ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag
    14581 tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc
    14641 atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct
    14701 atatattatc gggcaataaa ttaaaaaaag ctttacctta caataagtaa gataattttt
    14761 gtgataaaaa aataacgaaa aatatttaaa aatagtgtct atttcgtgag aaaacaacta
    14821 tatttttt
//
LOCUS       DADJYG010000413        11329 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.083, whole genome shotgun sequence.
ACCESSION   DADJYG010000413 DADJYG010000000
VERSION     DADJYG010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.083"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            292..567
                     /locus_tag="JZ382_002992"
     CDS             292..567
                     /locus_tag="JZ382_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429087.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2652
                     /locus_tag="JZ382_002993"
     CDS             580..2652
                     /locus_tag="JZ382_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAY2429088.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2817..4703
                     /locus_tag="JZ382_002994"
     CDS             2817..4703
                     /locus_tag="JZ382_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAY2429089.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4715..5662
                     /locus_tag="JZ382_002995"
     CDS             4715..5662
                     /locus_tag="JZ382_002995"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY2429090.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5682..6209
                     /locus_tag="JZ382_002996"
     CDS             5682..6209
                     /locus_tag="JZ382_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAY2429091.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6272..6925)
                     /locus_tag="JZ382_002997"
     CDS             complement(6272..6925)
                     /locus_tag="JZ382_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY2429092.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7057..7899
                     /locus_tag="JZ382_002998"
     CDS             7057..7899
                     /locus_tag="JZ382_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAY2429093.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8057..8953
                     /locus_tag="JZ382_002999"
     CDS             8057..8953
                     /locus_tag="JZ382_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAY2429094.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8956..9669
                     /locus_tag="JZ382_003000"
     CDS             8956..9669
                     /locus_tag="JZ382_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAY2429095.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9689..10207
                     /gene="msrA"
                     /locus_tag="JZ382_003001"
     CDS             9689..10207
                     /gene="msrA"
                     /locus_tag="JZ382_003001"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY2429096.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10204..10431
                     /locus_tag="JZ382_003002"
     CDS             10204..10431
                     /locus_tag="JZ382_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAY2429097.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10583..11134
                     /gene="lepB"
                     /locus_tag="JZ382_003003"
     CDS             10583..11134
                     /gene="lepB"
                     /locus_tag="JZ382_003003"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY2429098.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11217..>11329)
                     /locus_tag="JZ382_003004"
     CDS             complement(11217..>11329)
                     /locus_tag="JZ382_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAY2429099.1"
                     /translation="FLGRFIRNMIQIPIQIMVVYSFIKTLEARKVFKRAV"
BASE COUNT         3928 a         1917 c         2324 g         3160 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca aaacatatca
     2701 gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca aataccaaaa
     2761 aaattttcct gaagttcact atttttatga tacaatatcc aagataagga gggaacatgt
     2821 tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat ttgtttgacc
     2881 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
     2941 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
     3001 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
     3061 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
     3121 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
     3181 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
     3241 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
     3301 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
     3361 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
     3421 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
     3481 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
     3541 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
     3601 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
     3661 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
     3721 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
     3781 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
     3841 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     3901 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     3961 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     4021 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     4081 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     4141 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     4201 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     4261 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     4321 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     4381 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     4441 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     4501 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     4561 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     4621 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     4681 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     4741 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     4801 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     4861 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     4921 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     4981 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     5041 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     5101 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     5161 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     5221 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     5281 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     5341 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     5401 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     5461 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     5521 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     5581 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     5641 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     5701 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     5761 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     5821 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     5881 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     5941 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     6001 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     6061 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     6121 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     6181 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     6241 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     6301 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     6361 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     6421 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agccacccca
     6481 taaaaatata gatgatcgta gatacttttg atactctctc ccgtttatgc aacgtcaacg
     6541 ctttatatat aatgccaaga atcgcaaaca accagattaa tacaaataaa gtccaaccta
     6601 gccatcctcg gatactcagc aaacaaaaag gagtataact tcctgcaatc aaaagaaaaa
     6661 tcgaactatg atcgaatact tggaacacct tttttgcttt tgtaaaaatc aatgaatgaa
     6721 acaaagtaga cgttaaaaat aaaagaatca tcatcgatcc atagatagca taagagacaa
     6781 tatggaggga ggaatgcaag tgggcacctt ttactaaaag aaccactagt cccgcgatac
     6841 tcaatccagt cccaattcca tgggtgactg cattaaacac ttcatttaat atttcatacg
     6901 ttcgattaaa tttcgttttt tccatgaaaa cggctccttt actaggtgta attaatgaaa
     6961 aatcattatt tgtctgttat actaaaaata gaaattgtct tacatgtagt ttaacatgaa
     7021 taaggcatct tgttaaagaa agagagagag aaaataatga caaatgtaaa aatcgtaaca
     7081 gattcttcat gtacgatgga aaaaaatgta cgcgatgctt taggcattcg tgtcatgccg
     7141 ctatccatca tgatcgacgg aacagtatat cccgatgatg accaactaga cggcgaaaga
     7201 tttatggaaa tgatggcgaa agcaaaaaat ctgcccaaaa caagccaacc accaattgga
     7261 gaattcatct cgatgtatga tgagctagga gaagacggca gtgaagtgat ttcgatccat
     7321 atgacaaaag gcttgagtgg aacagtagaa gcagcaagac aagccagtaa tctaacaaaa
     7381 acgaaagtaa cggttatcga cagtgatacc actgaccaag gattatcttt tcaggtaatt
     7441 cgagcagctg aattagcaaa agaaggtaaa acagtagaga aaatccttga agaagtcaaa
     7501 aaagtacaag acaatacaaa attatacatc ggaatatcca cactagataa cttggtaaaa
     7561 ggtggaagaa tcagccgaac aactggtttg ctgtctagtg ttttgaacat acgcgtggtg
     7621 atgaatttcg cccattctga attaatccct gtaactaagg ggcgaggaaa gaaaacgttc
     7681 gataaatggt ttaataattt gaaaaatgaa ctgaaagaat tgccaaatgt gcgacaaatc
     7741 ggtatttcac atgcggatgc aaaagaattg gcgcaagaat tcaaagaggg tttgcaagaa
     7801 ttattcccgg acatgcatat ccctgtactg catacgaatc cggttatcgc tacgcatact
     7861 ggtaaagacg cgtttgcaat catgtattat gtagattagt cgaaaagagg ataagccctc
     7921 agcctgaaag gcttgcagct tattctcttt ttctttataa aattaggaaa ataattgttt
     7981 tcttttttca taaagactac ctttttgata aaatttttga taacattaga accaatcgaa
     8041 tacaagaggt gaaaaaatga aattaacgaa acgacatttg atcctcgttt tgttcccatt
     8101 attaattgcg atcatcatct atgcaggttt agctgcttct ataccgaaag ctggcccact
     8161 aatgaaacca gtaactactg tgagagaaga tccaaataaa aaagaaaaac ttcactacat
     8221 agctatcgga gattcgctga ctgaaggagt aggagatcaa accaagcaag gaggattcgt
     8281 tccccttgtc gcaaatgatc taaaagaccg ttataatttg accgcggttg aaacagaaaa
     8341 ctatggtgta aatggtgaac gaagtgacca gatcttaaag cgagtaaaaa caaaagaaga
     8401 aattcgaaac aatatcaaaa cagctgattt tatcacactg actgttggcg gtaatgattt
     8461 gatgaaagtt atccaaaata atttattcgg gttaacggtc aattccttta aaaagccact
     8521 gaaaaactat caggaaaatg tcacagaact attggaagaa tttcgatccc tcaataaaac
     8581 cgcacctatt tacattttag gcatctacaa tccattttat ttgaactttc ctgagatcac
     8641 tgacatgcag acaattgtta acaattggaa caaaggaaca gaaacggtga caaaaaaaat
     8701 gccgaactgc tactttatcc caatcaatga tttgctttat caaggattga atcaggaagt
     8761 aggtgtcaca gaagaaggaa cagatcaaac aactagcacg acagaaaatc taaatggcat
     8821 caaaaataat gtgctctatg aagaagatca tttccatcca aataatttag gttatcaatt
     8881 gatggcaaat gcagtgagag atcagctgat cgagacacaa aaccaatggt tagagaagga
     8941 ggatgatcaa taaacatgaa aacaagatct gacgtatctt caaacaaaac aaagaaaaca
     9001 aattactgga aattcgcctt ccttatcctt cttggggtag tacttggttc gaccgtattt
     9061 ttaggcagcc gtatttttgc taatcgggaa ccagaactcc cagaaatacc agctcttacg
     9121 gaaagacaag gagatcctgt cttaacgatc aattcaaata aagaaaaagt caatcaaatc
     9181 atcagctttt tccttagtga gtatcaaaaa gatagtgata ttgaatataa attttatttg
     9241 gaaaatgaag cactactcaa tggtactttc gaagtactag gttttcctat cgatttctat
     9301 ttatattttg atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc tagaagtctt
     9361 tctatcggaa cattgagttt gccaatcaaa gatgtgatga atatgatcaa gagaaactat
     9421 aaattaccag attggatcga agtcaataca gatgatctga ctatcatgct taggttggat
     9481 caattccgaa tgcaaaacgg gatgtatatt aaagcagata aaatcgactt agtaaatgat
     9541 gacattcggt ttagtctata tttgccaaaa gatacctctg atgcttcaaa caactcaaat
     9601 accaacaaaa ctagcaccag cagtactgaa agcaagacaa aagaaaataa aaaatcatcg
     9661 gatgagtagt attagagggg gaatcataat ggaacgagca atatttgcag gaggctgctt
     9721 ctggtgtatg atccagcctt tcgacacgct tcctggtatc catacagtga tgtcaggata
     9781 tactggaggg catgtaccag atccgactta tgaacaagta aaaagccaaa cgactggaca
     9841 tacagaagca gtggagatta tctatgatcc aacattgatt tcatatgaac aattagtaga
     9901 actttactgg cagcaaactg atccaactga cgctttcgga caattcgagg atcgcggtga
     9961 caattatcgc ccggtgatct tttatttgac agaagaacag cggatcattg ctgaaaaaag
    10021 taagaaacag ttagctgaaa gtggacgttt cgaacagcca atcgttacaa aaatcgaacc
    10081 agcccaacca ttttatctag cagaagaaga ccatcaagag tattataaaa agcatcctga
    10141 aaattttaaa agaaaccatg cacgccgggc tgcctttatc gaagaaaatt ggaggaacga
    10201 agggtgaatc gaagcttttt tcattattta atgacactgc gaggcggtgg cacacatgat
    10261 gctttatctg ttttcgctac agaagccggt aaagacatcc aatttcctaa gcacagctct
    10321 agctacgaag aaatttctaa ttatctggaa ctgaatgtcg actatttgcc gtccatggat
    10381 atctttgacg aagcatggga aaaatattta gaaaataatc atgacatata agagatagtt
    10441 ttgtacttta tgatcaaaaa agttttttgt ccaatagcct aattgaaaga attattttta
    10501 tttcagctat ctagtcgcat ttttttgata tttttcttat aataaagaaa gaatgaaaaa
    10561 aagagggatg gattgataga gattgacaaa aaaacaacgc tatattaatc gcttctggct
    10621 tgtattcaaa tacttacttg tttctgtatt ccttgcgttc atgttgcggg gatttttatt
    10681 catcccggta ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag gtgatatggt
    10741 tgtgatggaa aaattttcgg agatcagacg atttgatatt gttgtttttc aattagcaga
    10801 tggcacgatt tatatcaaac gagtaatcgg tcttccagga gaaaatgtca gctaccaaaa
    10861 tgatcaattg aagatcaatg ggaaagtagt taaggaacct tatctaacta aaaacctgaa
    10921 atcagatcat gcaaatgcat cctatacgac ggactttaca ctacaagagc taacgggaca
    10981 atccaagctt ccggaagata attattttgt acttggagat aatcgaagag tttcaaaaga
    11041 tagtcgttcg tttggcacga tcaataaaac tgatattcta ggtaaagcaa gatttgtcta
    11101 ttatccactt gatgaaataa aatggatcca gtgagaaaca ataaagcgtg cagtctgttg
    11161 agactgcacg ctttattgtt taatccaaaa tcagtttact accttagtac gtaaaatcaa
    11221 acagctcgtt taaacacttt tctagcctct aaagttttga taaatgaata aacaaccatt
    11281 atttgaatag ggatttggat catattcctg atgaatctac ctagaaaaa
//
LOCUS       DADJYG010000414        10027 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.098, whole genome shotgun sequence.
ACCESSION   DADJYG010000414 DADJYG010000000
VERSION     DADJYG010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.098"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(187..1059)
                     /locus_tag="JZ382_003005"
     CDS             complement(187..1059)
                     /locus_tag="JZ382_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY2429100.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1187..1756
                     /locus_tag="JZ382_003006"
     CDS             1187..1756
                     /locus_tag="JZ382_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAY2429101.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1823..2560
                     /locus_tag="JZ382_003007"
     CDS             1823..2560
                     /locus_tag="JZ382_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY2429102.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2791..4059
                     /locus_tag="JZ382_003008"
     CDS             2791..4059
                     /locus_tag="JZ382_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAY2429103.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4546..5010
                     /locus_tag="JZ382_003009"
     CDS             4546..5010
                     /locus_tag="JZ382_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY2429104.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4994..5623
                     /locus_tag="JZ382_003010"
     CDS             4994..5623
                     /locus_tag="JZ382_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAY2429105.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5977..7455
                     /gene="msr"
                     /locus_tag="JZ382_003011"
     CDS             5977..7455
                     /gene="msr"
                     /locus_tag="JZ382_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAY2429106.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7502..7948
                     /locus_tag="JZ382_003012"
     CDS             7502..7948
                     /locus_tag="JZ382_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAY2429107.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8147..8662)
                     /locus_tag="JZ382_003013"
     CDS             complement(8147..8662)
                     /locus_tag="JZ382_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY2429108.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8779..9981
                     /locus_tag="JZ382_003014"
     CDS             8779..9981
                     /locus_tag="JZ382_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY2429109.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3201 a         1753 c         2078 g         2995 t
ORIGIN      
        1 aacgtaatca attttatata gacagttatg ttttgtcaat tcttttttta taattatagt
       61 aatttactgg aattatcaga aataaaagga aggaacaaat taagaaactt atgttattta
      121 atcattggat cttttgctta acttattcgt tgttaaaaag ctgaaagcga aggaaaaaaa
      181 ctctacttaa ctgtatttct ccgttaggac atctaaaaat ttacgagcag caggagaaag
      241 aggctggtct tttttccaaa tgagattcag tgaagcttgt aatggcggat cgagtgggat
      301 gaatactttt tccgtcccat aagtattgat gatcccatct agagctaatg caccaaaagt
      361 cccgttatcg accatcaaag aagcattgta caataagtta tatgttccga cgatcgataa
      421 ctgatctatg ctgaaaccta gccattcagc tagttgactg tccactgacg tttgccggga
      481 aatgatcaaa ggaacagttt tcagttcctg cggatggatg actgtttgct ttgctaaagg
      541 atgattccga tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa atgactggta
      601 tttttgtttg tttgtcggtt cgattacgag tccaaaatca agtaatccgc tatccagttt
      661 gtctatgata tcatctgcat tcccgctata taaatgaaac tggatatcag gatgcgcctt
      721 gatcaacgaa tgaaggctat cagaaatgaa agagattgct ttagtttcgc ctccgccaat
      781 gtagatttct ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa
      841 cgacaagatt tcttttcctt ttgaataaag atatcgacca gaatccgtca actgtatctc
      901 tcgggagcct cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt tcagttgttt
      961 tgagagagta ggttgcgtga tatgcagaga ttttgctgct cggctgatat ttttttctct
     1021 agctactgct aaaaaatacg tcagtatacg aatatccatt ttttccctcc tgtttttaaa
     1081 aaagcatacc actaagtata gttaaaagga atagttaagt atgttttata agtattagac
     1141 atcataagcc gtccgttata aagtaaggat aaaaggaggg agcaaaatga caagcctcat
     1201 cgaaaaacaa atcgtcaata aagagattcg agtaacggat ccgctctttg aagtgatcca
     1261 tcagatccaa gcggaaaacg aagagccttt agcagcgctg aatactgggt accatgaacc
     1321 agcagatatc cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac
     1381 tgttctgctg cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt
     1441 tatcaatcgt caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt
     1501 gattggtcct cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag
     1561 aggcttgtca gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac
     1621 tattctgcca ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac
     1681 gaaagatgtc ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt
     1741 cgaacggatt atttgaacag taagatgaaa taagagatag tgagtataag caagagattt
     1801 ttcaaaacag gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg
     1861 cgcttcaagc ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact
     1921 agttatagct gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc
     1981 tgagctatat attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga
     2041 tttagcgatg gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc
     2101 aaccgctcca ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat
     2161 catgggtgtg ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg
     2221 gcatatcatt tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta
     2281 ttgcggaaca aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca
     2341 aaataacatc aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac
     2401 catcagcgac aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattaac
     2461 atctgaagat atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt
     2521 cagcaacatg attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca
     2581 gagagaatta cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga
     2641 agaatattct tcatttacgt ctttttatgc agaaaaagca aatttattaa aaagaaacaa
     2701 atgttattta aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg
     2761 aaaagtttca taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt
     2821 attcttttag gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa
     2881 actgtcgcaa ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagaaagt
     2941 actttgcagt cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa
     3001 acaacagaac aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag
     3061 aatgcagaag aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa
     3121 gcagatttgt ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct
     3181 aattctcaac aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc
     3241 aatgacttat atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac
     3301 agtactttgg cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc
     3361 caatctgtta cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc
     3421 gcttttagaa aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag
     3481 acgacttctt ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac
     3541 tcttacaaag atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt
     3601 tctacattga acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact
     3661 tcaacttctc cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg
     3721 aatgccggag aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg
     3781 caagcaccaa acagcggaca acctgtctac cgtttataca atccttacag tggagaccat
     3841 cattacacaa tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa
     3901 ggtctaagtt tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa
     3961 aaaactggta cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg
     4021 ggctggagat atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg
     4081 ccctgcatca agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt
     4141 tgaacttatt accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca
     4201 acatcttgtc ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg
     4261 acgggattct tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt atctactgca
     4321 gaagagctat cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt
     4381 ctttatcatt ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga
     4441 gagcgttttt tttattacac agctgattct ttgacttttt tcttttttta ttctattatt
     4501 gacaagtcaa tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag
     4561 attagtcacc ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa
     4621 gaaaatatcg gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat
     4681 ccattgctac aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt
     4741 catctgaagg tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac
     4801 cgggaagtgt atgtagcgct aacagaaaaa gcccagaaga aaatcaagca ttgtgaaatg
     4861 gaccataaag atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa
     4921 tttctaacac ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa
     4981 gaggttaata ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc
     5041 cgttcgcgta tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa
     5101 agaagcgacg actgcacctt cttctgtcaa tatgaaacca tggcgctttg tggtcgctga
     5161 aagcgaggaa gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga
     5221 tacttcttct gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga
     5281 aatctacaac caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt
     5341 agctgcgatc attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa
     5401 agtcgattct agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga
     5461 aacaaatcca attggtggat tcgaagcaga tcagttggca gaggcatttg gtttggataa
     5521 agaccgttat gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga
     5581 gtctgttcgt ttagcaccag aaaagatcac tatgttcgaa taaaaggttt tcagttacta
     5641 ttcagtagct attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga
     5701 aaaatgactg catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca
     5761 aaagacatgt ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt
     5821 agaataggtg aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta
     5881 ggaaaagatg tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat
     5941 aattgttgtt ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata
     6001 acaaatctac aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc
     6061 tatcagcaag atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc
     6121 aaactaatag ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc
     6181 aactacctgc ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc
     6241 agtcatttcc agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa
     6301 cgaaagatcg agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag
     6361 ccaacaaccc atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat
     6421 tatggtacgc tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa
     6481 atctgggaag taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc
     6541 actcaaaagg aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag
     6601 aaaaaacgat tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa
     6661 gtgtccagta aaaagaaaaa gcaacaaatc agactggatc ggttgtcttc ctctaaacaa
     6721 aaagacagtg tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa
     6781 aaaataggag aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa
     6841 tctcttgaac tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt
     6901 gggagaacat tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt
     6961 gtcggtgaaa atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa
     7021 ggaatccttc tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg
     7081 acatctgaag aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt
     7141 gtccgtagtt tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt
     7201 acattaagtg ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt
     7261 aatgtgttgc tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta
     7321 gagaagctaa tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt
     7381 gtcgagcgta cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt
     7441 acgagaaatt tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag
     7501 attgcctaca aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt
     7561 cttggggatg agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt
     7621 gattggtcta gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt
     7681 gctggcgaaa aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat
     7741 cttaactatt tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt
     7801 gatcatggaa caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat
     7861 gaatgtgatt gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt
     7921 gctaaaagca attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc
     7981 atgctttttg tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg
     8041 ttttggcctg tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga
     8101 taatttgctc ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact
     8161 gctgcatttg ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc
     8221 tgcgctggat cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca
     8281 attttttttg atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc
     8341 cagtagctgt tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt
     8401 tttgcgtagg agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat
     8461 cagtcaagtg gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac
     8521 cctgctggtc taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa
     8581 ttgtgatcag ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata
     8641 atatataggc taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc
     8701 acagtaaagt ttccacgttt tacatgtgca tattgacatg taaaataaat catgtatgat
     8761 gctaatagag agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca
     8821 aatgaagaaa caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct
     8881 tttatgggag tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct
     8941 acgccagctg aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg
     9001 ctatttacag gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg
     9061 tttatcatcg ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt
     9121 gggttacgtg ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg
     9181 atcgtcagta ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt
     9241 ggctgcggaa tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt
     9301 tatccatttt ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt
     9361 ttacctgaaa ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta
     9421 agcaatcata aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt
     9481 actttacttg cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt
     9541 gtattttttg gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt
     9601 gagcggaagt ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt
     9661 ttgctgttat taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta
     9721 gcaggatttt tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca
     9781 ggtctgcaac gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct
     9841 ttagcaccat ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat
     9901 tttgcaggtg ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt
     9961 gctacaaatg aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc
    10021 ataagaa
//
LOCUS       DADJYG010000415         9226 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.103, whole genome shotgun sequence.
ACCESSION   DADJYG010000415 DADJYG010000000
VERSION     DADJYG010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.103"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="JZ382_003015"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="JZ382_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAY2429110.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="JZ382_003016"
     CDS             2039..2227
                     /locus_tag="JZ382_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAY2429111.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="JZ382_003017"
     CDS             complement(2285..2638)
                     /locus_tag="JZ382_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY2429112.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="JZ382_003018"
     CDS             3169..3591
                     /locus_tag="JZ382_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAY2429113.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="JZ382_003019"
     CDS             3588..3818
                     /locus_tag="JZ382_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY2429114.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="JZ382_003020"
     CDS             4316..4516
                     /locus_tag="JZ382_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAY2429115.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..5736
                     /locus_tag="JZ382_003021"
     CDS             4543..5736
                     /locus_tag="JZ382_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY2429116.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="JZ382_003022"
     CDS             complement(5804..7369)
                     /gene="guaA"
                     /locus_tag="JZ382_003022"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY2429117.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7428..8351)
                     /locus_tag="JZ382_003023"
     CDS             complement(7428..8351)
                     /locus_tag="JZ382_003023"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY2429118.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8547..9152
                     /locus_tag="JZ382_003024"
     CDS             8547..9152
                     /locus_tag="JZ382_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY2429119.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
BASE COUNT         3037 a         1642 c         1764 g         2783 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat gtgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5341 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5401 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5461 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5521 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5581 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5641 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5701 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5761 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5821 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5881 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5941 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     6001 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6061 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6121 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6181 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6241 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6301 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6361 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6421 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6481 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6541 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6601 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6661 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6721 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6781 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6841 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6901 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6961 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     7021 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7081 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7141 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7201 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7261 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7321 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7381 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7441 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7501 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7561 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7621 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7681 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7741 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7801 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7861 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7921 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7981 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     8041 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8101 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8161 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8221 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8281 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8341 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8401 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8461 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8521 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8581 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8641 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8701 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8761 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8821 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8881 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8941 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     9001 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9061 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9121 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9181 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttt
//
LOCUS       DADJYG010000416         6892 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.129, whole genome shotgun sequence.
ACCESSION   DADJYG010000416 DADJYG010000000
VERSION     DADJYG010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.129"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            137..1573
                     /locus_tag="JZ382_003025"
     CDS             137..1573
                     /locus_tag="JZ382_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY2429120.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENGIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTNYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIVAGDEELLESAKPDFLGINYYQTATNAFNPLDGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRIHYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1762..4209
                     /locus_tag="JZ382_003026"
     CDS             1762..4209
                     /locus_tag="JZ382_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY2429121.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLVAILLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQAQKQAA
                     KEERMKQRAEAKEARRLAAQEAAEKEAVEYEKKKAVNQQRKNTPDEWQEQENAEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGYANELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERIVQFVTDQQEAHYEEKMMPTDEVETAGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIDQEETSEPLPEDNDM"
     gene            4443..5510
                     /gene="rlmN"
                     /locus_tag="JZ382_003027"
     CDS             4443..5510
                     /gene="rlmN"
                     /locus_tag="JZ382_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAY2429122.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSAHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTAAK"
     gene            complement(5704..6029)
                     /locus_tag="JZ382_003028"
                     /pseudo
     CDS             complement(5704..6029)
                     /locus_tag="JZ382_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
     gene            6209..6757
                     /gene="aac(6')"
                     /locus_tag="JZ382_003029"
     CDS             6209..6757
                     /gene="aac(6')"
                     /locus_tag="JZ382_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAY2429123.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPDSQ"
BASE COUNT         2324 a         1161 c         1542 g         1865 t
ORIGIN      
        1 cgtaaattcc ttcttatttc ttgaagctga ccacttcttt cacaacttcc tttacataat
       61 tttattttgt tgtggaaggg gtttcgtgtt attctaaaag aggaacgatt gaaggttata
      121 ggaaaggaag agatgaatgg aacacaaaga acttacctca tttccaaaag attttctgtg
      181 gggctctgca tctgcagcgt atcaagtaga aggagcttgg caagaagatg ggaaaggcga
      241 atcagtctgg gatcgatttg ttcggattcc aggaaagaca tttaaaggaa caaacggtga
      301 tctggcagta gaccactatc atcgttataa agaagatatt gctttaatga aagaacaagg
      361 tctgaaagct taccgcttct ctgtagcttg gacacggatt tttcctaatg gaagaggaga
      421 agtcaatcaa gcagggctcg ctttttatga gagattgatc gacgaattga tcgaaaacgg
      481 tattgagcct atgttgactt tgtatcattg ggatcttcca caagcattgc aagatgaata
      541 taatggttgg gaatcacgtc aaatcattga agatttcact aattatgcag aaacattatt
      601 tgaggcattc cgcgggaaag tccattactg gattagtttg aatgaacaaa atatctttac
      661 ttcattaggc tatttattag cagcacatcc gccaggcgtg acggatccaa aacgaatgta
      721 tgaagtcaac catatcgcaa atctagcaaa tgcatctgtc atcaacaaat tccatgagtt
      781 agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg ccaaattatc caatcgacag
      841 taatcctgaa aatgttctgg cagccgaaaa tgcagaagat ctgatggcca attattggat
      901 ggatgtctac atgtggggta aatatcctat tgctgctatg agattcttag aagaaaaagg
      961 atgggcgcca acgattgtag ctggcgatga agaattgcta gaatcagcca agccagattt
     1021 ccttgggatc aattactatc aaacagcgac gaatgcattc aatccattag atggagttgg
     1081 agcaggaaaa atgaatacga ctgggaaaaa aggttcatca gaagaaacag gggttcctgg
     1141 aatgtataaa aaagttgaga atccatttgt cgaacgaaca aattgggact gggagatcga
     1201 cccagaaggc ttaaggatcg gattgcgtcg tatcacaagc cgttaccgca tgccggtact
     1261 gatcacggaa aatggcttag gagaatatga caagttgaca gaagacaaac aaatccatga
     1321 cgactatcga atccattact tacaaagcca tgtcaaagca atcaaagaag caatttcgga
     1381 tggcgcagaa gtattaggct attgtacatg gtcttataca gatttgctga gttggctgaa
     1441 cggttatcaa aagcgatatg gatttgtata tgtcgatcaa gatgaaacac aagaaggatc
     1501 tttggaacga tataagaaag acagttatta ctggtaccaa aaactgattg aagaaaatgg
     1561 agagaacctt tagttctcat gcttaaaaca agaaggtacg gctggatatg gccgtgcctt
     1621 tttttgataa aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga
     1681 aagcgtcagt tctttcaaac aaaactttag ttcccttgct tttttttgta taatgaaact
     1741 tgatgaaatg agggataata aatggcacaa aagaaacggc ctactaagaa aaggaaaaag
     1801 acgaaaaaaa cgaaaaagca aaaacagcag caagaacaaa tgctgacgat tggtgttgga
     1861 tttgccttga ttctatttgc tgtattcggc tttttaaaat taggtttttt aggaatattg
     1921 attgcaaacg gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta
     1981 gccgtattag gattttggat tgtcattaag aatacagagt tttcaattgg caaaaaccga
     2041 cgctggtttg gcggtatact cttttatttc ggtatcttat tattgctaca tgcccattta
     2101 ttcggcaaac tccatactgg ggaaccaaat atcatgggga cgacatggga cttattggct
     2161 agcgatataa aacaaagtca agtagataat aacgttggcg gagggatgat cggagcaatt
     2221 ctttatcatt tcacttattt cttgattgca cagcctggaa gttatttagt agccattcta
     2281 ttactggcgg gtggagcatt cttgttcagt aatttggaag gctatcagct gctgaatggt
     2341 atccaatcaa ttggagaacg ggtacaggaa ttattggaag gtgatcctgc caagcaagct
     2401 caaaagcaag cggcaaagga agaaagaatg aaacagcgtg cagaagcgaa agaagcgaga
     2461 cgcttagcgg cacaagaagc agcagaaaaa gaagcagtag aatatgagaa gaaaaaagct
     2521 gtgaaccagc aaaggaaaaa tactcctgat gaatggcagg aacaagaaaa tgccgaacct
     2581 gagcagttaa gttttgttcc aattgacagt tttcaagaaa atatccatcc tgcgaatcta
     2641 gaaaaaccag tcccagatac accaaaacag accaatacag cagaaggtta tgctaatgag
     2701 ttgccagagg atgacggtac ctcattagaa tttgagatcg aagcagaaca ggaaaatcaa
     2761 gattatgaat tgccgacagt tgacttgctt gattctatac caactgtgga tcaaagtgat
     2821 gagtataaaa aaattgaaaa aaatatcggt gtattggaac aaactttcca aagtttcggt
     2881 gtggatgcta aggtcgtcaa agctagttta ggcccatccg tcacaaaatt cgaagtccaa
     2941 ccagctgtag gggtaaaagt aagtaaaatc gtgaacttaa cagatgatat cgcacttgct
     3001 ttagcagcga aagatgttcg gatggaagcg ccgatcccag gtaaatcact gattggtatt
     3061 gaagtaccaa atggaaagat cagtatggtt tcctttagag aaatcatcga agctcagcca
     3121 aaccatccag ataaattact ggaagtaccc ttaggacgtg atgtttctgg acgagtacaa
     3181 acggctgatc tttcgaaaat gcctcactta cttgtagctg gttcgactgg tagtgggaag
     3241 tcagtagcaa tcaatgggat cattacaagt atattgatgc gtgctaagcc ccatgaagta
     3301 aaactgatga tgatcgatcc gaaaatggtt gaattaaata tgtacaacgg gatacctcat
     3361 ctgttgacgc cggtcgtaac gaaccctaga aaagcggcac aagccttaca aaaagtagta
     3421 caagaaatgg aagaacgata tgaaaaattt gcggcaactg gtgtaagaaa tatttctggt
     3481 tacaatgaat ttgttcagca gaaaaactta gaaaacggaa cgaaacatcc aaccttacca
     3541 ttcatcgtag taatcgtaga tgaattagct gacttgatga tggtagctag caacgaagtc
     3601 gaagatgcga ttattcgttt agcgcaaatg gctcgggcag cgggcatcca catgatccta
     3661 gctactcaac gtccaagtgt ggatgttatc acagggatca tcaaagcaaa tgtaccttct
     3721 agaatggcat ttgctgtatc aagtggtaca gattctcgga cgatcatcga ctctaatgga
     3781 gcagaaaagc tcttagggcg aggagatatg ctgttcttgc caatgggtga gaataaacct
     3841 atccgtgtgc aaggagcgtt catctctgac catgaagtgg agcggatcgt tcaatttgtt
     3901 acagatcagc aagaagctca ttatgaagag aaaatgatgc cgacagatga agttgaaacg
     3961 gcaggtgccc cagaacagcc acaagatgag ctgtttgagg aagcaaaagc acttgttgtt
     4021 gaaatgcaaa cagcaagtat ctcccttctc caaagaagat ttcgaatcgg ttacaaccga
     4081 gccgctcgac tcgtagatga actggaagct catggagttg ttggcccatc agaaggaagt
     4141 aagcctcgga aagtattcat tgatcaagag gaaacatcag aaccactgcc ggaagataat
     4201 gatatgtaaa acttgttcaa tgaacggttg aaaaacgaat aactatataa aaaagacaag
     4261 acatgatgat accaagagaa aaaatggctt ttttcccatc ttttctaata cggtacgatg
     4321 ttttgtcttt tttgattttt atgagatgaa agttctattc gatttatttg tgaagtattg
     4381 cattaactaa acgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
     4441 ctttggaaaa acaatccatc tatggcttga cgaatgaaga attaatcaat tggtttatag
     4501 aaaacggtga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
     4561 taagtaactt cacagaaatg acgaatctat caaaacaatt gatagaaaaa ttatcggcac
     4621 attttatcat caatcctctg aaacaaatgg tcgtccaaga agcttcggac ggtacagtaa
     4681 aatatttgtt tgaacttcct gataaaaata tgatcgagac cgtactaatg agacaagaat
     4741 acggcttgtc cgtttgcgtg accacacaag taggatgtaa tatcggctgt acattttgtg
     4801 caagcgggtt gcttaaaaag aaccgtgatt tgaccgctgg agaaatcgtc gcgcagatca
     4861 tgatggttca acattatttt gatgagcgca atcttggcga acgtgtttcc catgtcgtcg
     4921 taatgggaat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatta
     4981 ttaatgatgc caaaggattg gcgattggtg cgcgacatat tacagtttct actagtggct
     5041 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtaaatctg gctatttcct
     5101 tgcacgcgcc gaataatgag gtgcgaacat cgattatgcg gatcaatcga agtttcccaa
     5161 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
     5221 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
     5281 atctattaaa agataagaaa aagttagcat atgtcaactt gattccatat aatccagtca
     5341 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
     5401 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
     5461 cttgcggaca attgcgaagc aagcaaatga aaaaaacagc tgccaaataa tcgtagcctg
     5521 ctttgttttt tcaattacca taaaaggagc agttttatta attaaggaac tgtccaactt
     5581 aaataagtga aaagattcta tagcgaaagt tataaagcgt tttttgacaa gaaaagccct
     5641 catcaagcaa agaaatgtcg tcaatttgat gacagcctgc ttgatggggg ttttagcaat
     5701 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
     5761 gtcaagaatg gcctgtttaa atttttctcg ggagatttca taaggcacgt gtttgatatc
     5821 atttgtggtc atggctttat ctaagattgc ttctaaatcg gtcgtgatat tgagattcat
     5881 gtcggttaac gatactggaa gttgtttttt tcataaacac atagatttta tcaaacaaat
     5941 cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa
     6001 ggtatccctc catttttttt agaggtcgta ctgcaataga atatgtgtac cttagcagaa
     6061 atagtatttc tggagttttt ttttgcttta taagcagggc agccgattag tttgctagag
     6121 aaataattga ctgctaaaga atttgagcga gaacatttcg ttttttcaaa aggactgtta
     6181 gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc
     6241 agtattgaaa gaccagcttt ctgatttact gagactgact tggccggaag aatatggaga
     6301 cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt
     6361 agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg
     6421 ggaattgcat ccattagttg tagaaagctc tcgacgaaag aaccaaatag gtactcgatt
     6481 agtcaattac ttagaaaaag aagtagcttc cagaggagga ataacgattt atttaggtac
     6541 ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacgtttga
     6601 taaagtggct tccatccaga accttcgtga acatccgtat gagttctatg aaaaattagg
     6661 ttataaaatc gtaggtgtct taccaaatgc aaatggttgg gacaaaccgg atatttggat
     6721 ggcaaaaacg attattcctc gaccagattc tcaataataa aaagaggttg tgaaatcagc
     6781 gtcctgacct gagtattagc tcaacaaacg gaaaaatagt tcttcatatt ttgccgtttg
     6841 ttgagcgaat atcgagggtc agctgattga acaccgttta gtggagaggt tg
//
LOCUS       DADJYG010000417         5986 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.140, whole genome shotgun sequence.
ACCESSION   DADJYG010000417 DADJYG010000000
VERSION     DADJYG010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.140"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            complement(41..1027)
                     /locus_tag="JZ382_003030"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="JZ382_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="JZ382_003031"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="JZ382_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAY2429124.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="JZ382_003032"
     CDS             1852..3006
                     /locus_tag="JZ382_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAY2429125.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="JZ382_003033"
     CDS             3221..4189
                     /locus_tag="JZ382_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAY2429126.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="JZ382_003034"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="JZ382_003034"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAY2429127.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="JZ382_003035"
     CDS             5219..5827
                     /locus_tag="JZ382_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAY2429128.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatccc
//
LOCUS       DADJYG010000418         3158 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.210, whole genome shotgun sequence.
ACCESSION   DADJYG010000418 DADJYG010000000
VERSION     DADJYG010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.210"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            117..614
                     /gene="dfrG"
                     /locus_tag="JZ382_003036"
     CDS             117..614
                     /gene="dfrG"
                     /locus_tag="JZ382_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAY2429129.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(686..2638)
                     /locus_tag="JZ382_003037"
     CDS             complement(686..2638)
                     /locus_tag="JZ382_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAY2429130.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2645..2941)
                     /locus_tag="JZ382_003038"
     CDS             complement(2645..2941)
                     /locus_tag="JZ382_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY2429131.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          961 a          555 c          522 g         1120 t
ORIGIN      
        1 taatataact tatagaaaaa attgcttatt caactaacgg gcaggataat gagaaaaggt
       61 tttttgatag aaataagaaa ttagatggta aattagttaa aaaggctgtg ttataaatga
      121 aagtttcttt gattgctgcg atggataaga atagagtgat tggcaaagag aatgacattc
      181 cttggaggat tcccaaggac tgggaatatg ttaaaaatac tacaaaggga catccgataa
      241 tattaggtag gaagaacctt gaatcaatcg gaagagcctt acctgacaga agaaatatta
      301 ttctgacgag agataagggg tttaccttta atggttgtga aattgttcat tcaatagaag
      361 atgtttttga gttatgtaaa aacgaagaag aaatttttat tttcggagga gaacagattt
      421 ataatttgtt tttcccttat gttgagaaaa tgtacatcac aaaaatacat catgaattcg
      481 aaggagatac tttttttcca gaagtgaatt atgaggaatg gaatgaggta tttgcccaaa
      541 aagggataaa gaatgataaa aatccgtata actactattt tcatgtatat gaaagaaaaa
      601 acttattgag ttaacgggtg cgttaattga agaagaacag taaagataaa gcacaagagc
      661 ttgggattac tccaagctct ttttgtcatt tgaaataaat tatatctttc caatcaaatt
      721 gtttatctgt aatccctaat ctttgagcag gtgtcaaaac ctttttatcc gctgatttaa
      781 aagggcgaca aaagttgtag taagttctta gaattgtaat cgcaaattga gcatacttcg
      841 gattgaagtt agcgtaaata tagctcttct tatcaccacg agctgttgtt aatggtcttt
      901 caagagatga tatataacgt cttatctgtt gaataaagct attggtcgaa tggtcattaa
      961 catttaatag catctttgcg atttcgtttg gttccagagc agataaatcc gttctacaat
     1021 caactgaata aaatccttta tccttagtgg ctaatggatg tttgataggg ttatcggcat
     1081 atttgcgata tctttctcca tctttactaa tagcctcttc gtggaattga tgacttgtta
     1141 gtaattcagt tagataatga gatgccaaag tccgtagaaa ctttgttttg aatcctctga
     1201 tatctcccca atcgagcagt tctgcttttg cttgtccgaa ttcttttaag cattgttttc
     1261 tgcttttcgt cttgttaact tggcaaataa aatgatgtgc atcgctcaac cttaattctt
     1321 tcgtgaatac tctataaaag gcgtttctaa tagagaagtc atcatcacta atcattcgcc
     1381 attcgtcggc attaaccatt tgtttaatta accaatagtg agcagttgta gtataaggag
     1441 catcaacatt taaaccatcg acaaatctac tcctattagt aattttccct aattcgtgaa
     1501 gataagcatt cctattttcg ctatcgttag cggatggttc ttgtggataa taagaccaat
     1561 ctaacctatc atttttacga ctaaaagtat tgagatggtc ttctttgaat tttcttgtat
     1621 cttcatttaa ttcatccatt gaaatattcc aatcataggc aacatcagaa cggaacacat
     1681 atcttgataa gacctcagca gatacaacta tatacgtttg catattaagg tcttcaaacc
     1741 ccgtatattt ctttgaacct tgtcctttct tacggacgtt atttaggtag tagtgcatct
     1801 tgtccgtatt aagccacatc tcgttaaatt tctttgtttg aaggggttgt gtctcatatc
     1861 gttctaaaaa ctccaaacaa cgcctataca gccactccag cttatgatag taggttccaa
     1921 ctccaattcc taaaatatca caagtacgac tgactgaaac ccttccaaca accatcttag
     1981 cgaacattgg aagaatggtg ttcttttgtt ggtgataagt agtggtttct tctctttttg
     2041 gtagaacatt agtgaacttc ttacacgcct tacattgata gcgttgagat ttgcccctac
     2101 ttttccccct tttgtaaaat tgcttcggct cattgaatgg ggttgattct tcttccgagc
     2161 agccttcttt atgaaattga taatctggct caacatcttg gatgctattg atttctataa
     2221 ggcgtttaat ttcttcaaca acagaccaat tagataaagg ggttactgta cagttttgcg
     2281 atacacctct gccaatagga ttgtcattgc agtacatacc tttatgacct ttatctttgg
     2341 aactaccagt cattttatat cgacttggtt tcccttttac atctttgaat ttgtgctgtt
     2401 ctttcccaaa attacaacaa aacggattga tgcagtaatt atattgaatg ggatattcag
     2461 aaccgttcca tttaaaagac gttggcaaga acaacatttt gttatacctt gttgcaaatc
     2521 gttttggagt taataaatca tattgtttag ccctatcttt taattcttcg tttgaaatcg
     2581 gaattttaac atctacaatt tcatcttcct tcgtggctaa tcttttaagc ttggtcattg
     2641 tctatcaacc cactctatct ttgataccca ttttttcaac aatccgctta ttacttcgct
     2701 tgttggcaat ccattcaata aacttcttat cgtctaaaat gttcaataag aattcatcca
     2761 ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg cttaactatc acccttactt
     2821 gctctgatat ttcccaatcc acagacttag cgttcttatt ttttggctca agaaattgca
     2881 taggaaaatg cacccctttt tgcttagtaa attgtaacca ttttaacaca ccaatttgca
     2941 ttcctacaat aattatttca cgttcgcatt tttatataca ttttaactta cattttgtgg
     3001 tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg agtatcaaat tcagttgcaa
     3061 tttgatactc atttaggttt acattttcac tttttgtact ccaaaactga actacttaat
     3121 atgcaagacg ttgtcttttt tttgtatatt caattttg
//
LOCUS       DADJYG010000419         2951 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.219, whole genome shotgun sequence.
ACCESSION   DADJYG010000419 DADJYG010000000
VERSION     DADJYG010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.219"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            10..792
                     /locus_tag="JZ382_003039"
     CDS             10..792
                     /locus_tag="JZ382_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAY2429132.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(918..1649)
                     /gene="erm(A)"
                     /locus_tag="JZ382_003040"
     CDS             complement(918..1649)
                     /gene="erm(A)"
                     /locus_tag="JZ382_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAY2429133.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1707..1794)
                     /locus_tag="JZ382_003041"
                     /pseudo
     CDS             complement(1707..1794)
                     /locus_tag="JZ382_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2158..2820
                     /locus_tag="JZ382_003042"
     CDS             2158..2820
                     /locus_tag="JZ382_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY2429134.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT          972 a          431 c          550 g          998 t
ORIGIN      
        1 ggtgaaatca tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta
       61 aaaatcgtaa aagatttatt tggaagttca atagttggag tatatctatt tggttcagca
      121 gtaaatggtg gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt
      181 ttacctcaat taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt
      241 ggaaatacgg attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc
      301 ccttggcaat atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt
      361 gagaatggac aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa
      421 gcaagaaaga atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta
      481 cctttgacag atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata
      541 aaaggtgatg agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact
      601 ggtgaaatta cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag
      661 catgtaactt tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg
      721 gaaggactat attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact
      781 tctctcaatt aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt
      841 attgataaat attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa
      901 cgctttatta ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt
      961 tccttcgata gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg
     1021 tttttagtga aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac
     1081 tttttgtagt ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag
     1141 tctacacttg gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc
     1201 atctccacca ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat
     1261 cccttctcaa cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca
     1321 atatccgtac tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg
     1381 aaggaaaatt ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct
     1441 tctttagtca cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt
     1501 ttgactagct ctttggtaaa atgtcctttt cctgatccga tttctattac gttgtcttgt
     1561 ttactgatat tcgtgtgatt caatatttct tttacatgct ttttagaagt aataaaattt
     1621 tgcgtgtctt tagggttttt ctggttcatt ataaccttct ccttactggt tataatgaac
     1681 tgacattaag cgttcattat aaccaattaa ttttgatttg gttgataacg aactttatta
     1741 ataacaaata tagaaaaagt acccatactt ttatctcctt tccatttttt tcattatgaa
     1801 tgagataaag taatttctac tactgcgata ctggtgcaca taattgaaac ctccttatgt
     1861 aactgaatat gattgtatca tcttttcatt ttagaataaa cctttatatg taattttgca
     1921 cttgacactt cagatggacg ttttcttcaa aataatatgt tttgaaactt gataagagaa
     1981 gccactgtct ttactaaaaa gtaaaggctg cgataaagga cagtgacttt aataaagttg
     2041 cactgttgta ttgtctttgg ataaaattgt ctaaagggaa gttttatccc caacgaaaaa
     2101 acatctaaag tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg gatctttttg
     2161 aatacaatag aatcacagtt tgataaggtt gcagaagatt acgatttcgt gaatgagctt
     2221 ttgaatgatt attcattctt tgtgtctaat atgtctccaa agaaaggcag agcattagat
     2281 atcggatgtg gctcgggttt gttagtggag aaattagcaa gttattatga tgaagtggta
     2341 gggattgata tttctaatca aatgctcgat cttgccaaat ctaaacgtca actaacaaat
     2401 acggtctatc tgaatatgaa tgcagaacaa cttaatttta atgagaagtt tgattttatt
     2461 gtaagccgaa caacctttca tcatttggat gatatagcca gcgtaataca gcaaatgaag
     2521 gaactgttga atgaggaagg aagaatagtt attcttgata atgtatctga agtcgagaca
     2581 ccgcctacct atgtttataa attaggggca atccaagagt ttttaccgca ttgtttcaaa
     2641 tttggaataa aaaatgcgat tagaatttat aatcacaaca catctaaatc atggcttgag
     2701 catttagctt cagataagta cctatctgaa caaaattatt acgatttata tgaaaagttg
     2761 ttgcctggat gccaattcca taaaatgggt tgggcaatgg gggttgtctg gacaaaataa
     2821 cttaaagctg tggctttgaa aagttgaatt tgtgattgtt ttcagagcta tcgggtgctt
     2881 tagtataaat aacaatgaat aaaaaatcag cctgtatctt atgttactat taagtaattt
     2941 aagatacagg t
//
LOCUS       DADJYG010000420         2036 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.265, whole genome shotgun sequence.
ACCESSION   DADJYG010000420 DADJYG010000000
VERSION     DADJYG010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.265"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="JZ382_003043"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="JZ382_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAY2429135.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          706 a          304 c          413 g          613 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat gtgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg
      661 ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt
      721 attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt
      781 ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga
      841 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      901 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      961 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatag
//
LOCUS       DADJYG010000421         1504 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.292, whole genome shotgun sequence.
ACCESSION   DADJYG010000421 DADJYG010000000
VERSION     DADJYG010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.292"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            262..373
                     /locus_tag="JZ382_003044"
                     /pseudo
     CDS             262..373
                     /locus_tag="JZ382_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="JZ382_003045"
     CDS             394..477
                     /locus_tag="JZ382_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAY2429136.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="JZ382_003046"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="JZ382_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAY2429137.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DADJYG010000422         1349 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.310, whole genome shotgun sequence.
ACCESSION   DADJYG010000422 DADJYG010000000
VERSION     DADJYG010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.310"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            46..297
                     /locus_tag="JZ382_003047"
                     /pseudo
     CDS             46..297
                     /locus_tag="JZ382_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="JZ382_003048"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="JZ382_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAY2429138.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DADJYG010000423         1194 bp    DNA     linear   BCT 01-MAR-2021
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00017934
            SAMN18085465-rid11676463.guided.322, whole genome shotgun sequence.
ACCESSION   DADJYG010000423 DADJYG010000000
VERSION     DADJYG010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN18085465
            Sequence Read Archive: SRR13803177
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-FEB-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000974455.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000974455.1
            Genome Coverage       :: 180x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/28/2021 08:40:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,049
            CDSs (total)                      :: 2,992
            Genes (coding)                    :: 2,924
            CDSs (with protein)               :: 2,924
            Genes (RNA)                       :: 57
            rRNAs                             :: 2, 3 (16S, 23S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 28 of 68
            Pseudo Genes (incomplete)         :: 33 of 68
            Pseudo Genes (internal stop)      :: 16 of 68
            Pseudo Genes (multiple problems)  :: 8 of 68
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN18085465-rid11676463.guided.322"
                     /strain="AUSMDU00017934"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2018"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="JZ382_003049"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="JZ382_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAY2429139.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
